LOCUS       DAZKMB010000001        79650 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000001 DAZKMB010000000
VERSION     DAZKMB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.001"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..914
                     /locus_tag="ACG8V3_000001"
     CDS             33..914
                     /locus_tag="ACG8V3_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0298579.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            927..2537
                     /locus_tag="ACG8V3_000002"
     CDS             927..2537
                     /locus_tag="ACG8V3_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0298580.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2614..3273)
                     /locus_tag="ACG8V3_000003"
     CDS             complement(2614..3273)
                     /locus_tag="ACG8V3_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0298581.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3556..4221)
                     /locus_tag="ACG8V3_000004"
     CDS             complement(3556..4221)
                     /locus_tag="ACG8V3_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0298582.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4368..4973)
                     /gene="dhaL"
                     /locus_tag="ACG8V3_000005"
     CDS             complement(4368..4973)
                     /gene="dhaL"
                     /locus_tag="ACG8V3_000005"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0298583.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5001..6011)
                     /gene="dhaK"
                     /locus_tag="ACG8V3_000006"
     CDS             complement(5001..6011)
                     /gene="dhaK"
                     /locus_tag="ACG8V3_000006"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0298584.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5986..6381)
                     /gene="dhaM"
                     /locus_tag="ACG8V3_000007"
     CDS             complement(5986..6381)
                     /gene="dhaM"
                     /locus_tag="ACG8V3_000007"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0298585.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6535..6855
                     /locus_tag="ACG8V3_000008"
     CDS             6535..6855
                     /locus_tag="ACG8V3_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0298586.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6967..7824)
                     /locus_tag="ACG8V3_000009"
     CDS             complement(6967..7824)
                     /locus_tag="ACG8V3_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0298587.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7932..9236)
                     /locus_tag="ACG8V3_000010"
     CDS             complement(7932..9236)
                     /locus_tag="ACG8V3_000010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0298588.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9233..9742)
                     /locus_tag="ACG8V3_000011"
     CDS             complement(9233..9742)
                     /locus_tag="ACG8V3_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298589.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9917..12052)
                     /locus_tag="ACG8V3_000012"
     CDS             complement(9917..12052)
                     /locus_tag="ACG8V3_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0298590.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12135..13790)
                     /locus_tag="ACG8V3_000013"
     CDS             complement(12135..13790)
                     /locus_tag="ACG8V3_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0298591.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13792..14106)
                     /locus_tag="ACG8V3_000014"
     CDS             complement(13792..14106)
                     /locus_tag="ACG8V3_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0298592.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14276..14536
                     /locus_tag="ACG8V3_000015"
     CDS             14276..14536
                     /locus_tag="ACG8V3_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0298593.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14550..14864
                     /locus_tag="ACG8V3_000016"
     CDS             14550..14864
                     /locus_tag="ACG8V3_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0298594.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15594..16148)
                     /locus_tag="ACG8V3_000017"
     CDS             complement(15594..16148)
                     /locus_tag="ACG8V3_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0298595.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16274..18203)
                     /locus_tag="ACG8V3_000018"
                     /pseudo
     CDS             complement(16274..18203)
                     /locus_tag="ACG8V3_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18509..19225)
                     /locus_tag="ACG8V3_000019"
     CDS             complement(18509..19225)
                     /locus_tag="ACG8V3_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0298596.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19226..21049)
                     /gene="glpO"
                     /locus_tag="ACG8V3_000020"
     CDS             complement(19226..21049)
                     /gene="glpO"
                     /locus_tag="ACG8V3_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0298597.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21063..22559)
                     /gene="glpK"
                     /locus_tag="ACG8V3_000021"
     CDS             complement(21063..22559)
                     /gene="glpK"
                     /locus_tag="ACG8V3_000021"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0298598.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22695..24155)
                     /locus_tag="ACG8V3_000022"
     CDS             complement(22695..24155)
                     /locus_tag="ACG8V3_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0298599.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24145..25440)
                     /locus_tag="ACG8V3_000023"
     CDS             complement(24145..25440)
                     /locus_tag="ACG8V3_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0298600.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25772..26449)
                     /locus_tag="ACG8V3_000024"
     CDS             complement(25772..26449)
                     /locus_tag="ACG8V3_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0298601.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26479..27570)
                     /locus_tag="ACG8V3_000025"
     CDS             complement(26479..27570)
                     /locus_tag="ACG8V3_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0298602.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27671..28105)
                     /locus_tag="ACG8V3_000026"
     CDS             complement(27671..28105)
                     /locus_tag="ACG8V3_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0298603.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28689..30146)
                     /locus_tag="ACG8V3_000027"
     CDS             complement(28689..30146)
                     /locus_tag="ACG8V3_000027"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0298604.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30143..32038)
                     /locus_tag="ACG8V3_000028"
     CDS             complement(30143..32038)
                     /locus_tag="ACG8V3_000028"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0298605.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32096..33871)
                     /locus_tag="ACG8V3_000029"
     CDS             complement(32096..33871)
                     /locus_tag="ACG8V3_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0298606.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33921..35417)
                     /locus_tag="ACG8V3_000030"
     CDS             complement(33921..35417)
                     /locus_tag="ACG8V3_000030"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0298607.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35611..37233)
                     /locus_tag="ACG8V3_000031"
     CDS             complement(35611..37233)
                     /locus_tag="ACG8V3_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0298608.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37561..38874)
                     /locus_tag="ACG8V3_000032"
     CDS             complement(37561..38874)
                     /locus_tag="ACG8V3_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0298609.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39119..39343)
                     /locus_tag="ACG8V3_000033"
     CDS             complement(39119..39343)
                     /locus_tag="ACG8V3_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298610.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39363..39785)
                     /locus_tag="ACG8V3_000034"
     CDS             complement(39363..39785)
                     /locus_tag="ACG8V3_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0298611.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39927..40637)
                     /locus_tag="ACG8V3_000035"
     CDS             complement(39927..40637)
                     /locus_tag="ACG8V3_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0298612.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40655..41152)
                     /locus_tag="ACG8V3_000036"
     CDS             complement(40655..41152)
                     /locus_tag="ACG8V3_000036"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0298613.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41189..41641)
                     /locus_tag="ACG8V3_000037"
     CDS             complement(41189..41641)
                     /locus_tag="ACG8V3_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0298614.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41776..42381)
                     /locus_tag="ACG8V3_000038"
     CDS             complement(41776..42381)
                     /locus_tag="ACG8V3_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0298615.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42831..43451)
                     /locus_tag="ACG8V3_000039"
     CDS             complement(42831..43451)
                     /locus_tag="ACG8V3_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0298616.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43453..44322)
                     /locus_tag="ACG8V3_000040"
     CDS             complement(43453..44322)
                     /locus_tag="ACG8V3_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0298617.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44345..44752)
                     /locus_tag="ACG8V3_000041"
     CDS             complement(44345..44752)
                     /locus_tag="ACG8V3_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0298618.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44771..46060)
                     /locus_tag="ACG8V3_000042"
     CDS             complement(44771..46060)
                     /locus_tag="ACG8V3_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0298619.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            46118..47457
                     /locus_tag="ACG8V3_000043"
     CDS             join(46118..46559,46559..47457)
                     /locus_tag="ACG8V3_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0298620.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            47526..48128
                     /locus_tag="ACG8V3_000044"
     CDS             47526..48128
                     /locus_tag="ACG8V3_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0298621.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            48150..48638
                     /locus_tag="ACG8V3_000045"
     CDS             48150..48638
                     /locus_tag="ACG8V3_000045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0298622.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            48960..50333
                     /locus_tag="ACG8V3_000046"
     CDS             48960..50333
                     /locus_tag="ACG8V3_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298623.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            50317..50754
                     /locus_tag="ACG8V3_000047"
     CDS             50317..50754
                     /locus_tag="ACG8V3_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0298624.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(51034..52779)
                     /locus_tag="ACG8V3_000048"
     CDS             complement(51034..52779)
                     /locus_tag="ACG8V3_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0298625.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            52975..54051
                     /locus_tag="ACG8V3_000049"
     CDS             52975..54051
                     /locus_tag="ACG8V3_000049"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0298626.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54204..55556
                     /gene="murF"
                     /locus_tag="ACG8V3_000050"
     CDS             54204..55556
                     /gene="murF"
                     /locus_tag="ACG8V3_000050"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0298627.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            55878..57389
                     /locus_tag="ACG8V3_000051"
     CDS             55878..57389
                     /locus_tag="ACG8V3_000051"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0298628.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            57519..57869
                     /gene="acpS"
                     /locus_tag="ACG8V3_000052"
     CDS             57519..57869
                     /gene="acpS"
                     /locus_tag="ACG8V3_000052"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0298629.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            57885..59006
                     /gene="alr"
                     /locus_tag="ACG8V3_000053"
     CDS             57885..59006
                     /gene="alr"
                     /locus_tag="ACG8V3_000053"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0298630.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            59017..59379
                     /locus_tag="ACG8V3_000054"
     CDS             59017..59379
                     /locus_tag="ACG8V3_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0298631.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(59555..59824)
                     /gene="rpsN"
                     /locus_tag="ACG8V3_000055"
     CDS             complement(59555..59824)
                     /gene="rpsN"
                     /locus_tag="ACG8V3_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0298632.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            60014..60961
                     /locus_tag="ACG8V3_000056"
     CDS             60014..60961
                     /locus_tag="ACG8V3_000056"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0298633.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            61267..63972
                     /locus_tag="ACG8V3_000057"
     CDS             61267..63972
                     /locus_tag="ACG8V3_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0298634.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            64570..66393
                     /locus_tag="ACG8V3_000058"
     CDS             64570..66393
                     /locus_tag="ACG8V3_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0298635.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(66535..66675)
                     /locus_tag="ACG8V3_000059"
                     /pseudo
     CDS             complement(66535..66675)
                     /locus_tag="ACG8V3_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            67196..67459
                     /locus_tag="ACG8V3_000060"
     CDS             67196..67459
                     /locus_tag="ACG8V3_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0298636.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            67459..67725
                     /locus_tag="ACG8V3_000061"
     CDS             67459..67725
                     /locus_tag="ACG8V3_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0298637.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            67926..68414
                     /locus_tag="ACG8V3_000062"
     CDS             67926..68414
                     /locus_tag="ACG8V3_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0298638.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(68955..69626)
                     /locus_tag="ACG8V3_000063"
     CDS             complement(68955..69626)
                     /locus_tag="ACG8V3_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0298639.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(69623..70540)
                     /locus_tag="ACG8V3_000064"
     CDS             complement(69623..70540)
                     /locus_tag="ACG8V3_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0298640.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            complement(70537..71178)
                     /locus_tag="ACG8V3_000065"
     CDS             complement(70537..71178)
                     /locus_tag="ACG8V3_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0298641.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            complement(71182..72356)
                     /locus_tag="ACG8V3_000066"
                     /pseudo
     CDS             complement(71182..72356)
                     /locus_tag="ACG8V3_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            72549..73580
                     /gene="queA"
                     /locus_tag="ACG8V3_000067"
     CDS             72549..73580
                     /gene="queA"
                     /locus_tag="ACG8V3_000067"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0298642.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            74199..76838
                     /locus_tag="ACG8V3_000068"
     CDS             74199..76838
                     /locus_tag="ACG8V3_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0298643.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(77006..77704)
                     /locus_tag="ACG8V3_000069"
     CDS             complement(77006..77704)
                     /locus_tag="ACG8V3_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0298644.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            77922..79067
                     /gene="tgt"
                     /locus_tag="ACG8V3_000070"
     CDS             77922..79067
                     /gene="tgt"
                     /locus_tag="ACG8V3_000070"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0298645.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            79164..79544
                     /gene="yajC"
                     /locus_tag="ACG8V3_000071"
     CDS             79164..79544
                     /gene="yajC"
                     /locus_tag="ACG8V3_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0298646.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
BASE COUNT        23668 a        15963 c        15014 g        25005 t
ORIGIN      
        1 tatatatttt tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt
       61 tgaaaaagag ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag
      121 gtgaagtcgt tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac
      181 ttttagggat tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg
      241 aagatagttt atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg
      301 gaaatttgac tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg
      361 ctcaaaaaaa agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa
      421 actattcaaa aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg
      481 atctctattt atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc
      541 aagaagaagt tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc
      601 tcagtgaagt cgagcgacta gcggataaag tcgtgatcat acgcgaagga acactcgatg
      661 aagaaggaac actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag
      721 aagcagagaa tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc
      781 acctcgctca atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca
      841 aattaggtgt gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac
      901 attatgaagg atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt
      961 tttctcaaaa ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg
     1021 ctttttctgg tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg
     1081 gattatacga aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt
     1141 cgaatgctgc tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca
     1201 gtttgtttgc tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag
     1261 aacttggtct gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag
     1321 ctgtcatcgc tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga
     1381 tgattttttt ccaagcggat tcgatcactg cagaaggtgc ttggcttttt gggctatctg
     1441 taggtagtat ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa
     1501 gttcagccag ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag
     1561 caataacaga tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt
     1621 tgacctatcc atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc
     1681 taatcttgat aatcagttca tttgttttag aggaacatcg agatttgaac accggctata
     1741 tccccgaacg tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat
     1801 tattctatct aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg
     1861 cggcatacgg ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga
     1921 aacagatgtt tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg
     1981 tagtcttgat ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag
     2041 aagaaaccca cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt
     2101 attggaccaa tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg
     2161 gtgcactagg gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga
     2221 tgtttcttaa agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg
     2281 gctttctatt cggatggctt cctcgaatcg gtaaacttgc ttatatctac ttagcttact
     2341 cctttgcact caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag
     2401 ctgtccctag ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta
     2461 cgttatcatg tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag
     2521 acttactaga aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt
     2581 caaaaatcca acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc
     2641 aatgatccag acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa
     2701 ttttctagat aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat
     2761 tggcaggaaa aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac
     2821 tcgttcttct actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt
     2881 ctcaacgttg attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga
     2941 gaaatctttt tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga
     3001 ctcatttgga acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg
     3061 taaaagctgt tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac
     3121 gatcactaat tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg
     3181 atgtttgaca agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg
     3241 aacaaatgaa acacataaat gatcatgact cataatctct cctccttcta aacagctatt
     3301 tctaataaag aaacataact tcccttattc caactgataa ttttctttta tcatagcata
     3361 aacgtcaagc gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat
     3421 tttccttctt ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt
     3481 tgaataatca aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg
     3541 cttatttcgt gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag
     3601 ttcttgtaag gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa
     3661 tagtttaccc atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt
     3721 tgtatcggac aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg
     3781 ccatatgtaa taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa
     3841 ctggttatac gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa
     3901 atagatttgg tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg
     3961 agattcgctt cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct
     4021 ttcgatttta ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg
     4081 aatctctttc catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc
     4141 tgttggatgg ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata
     4201 atctgatttt ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga
     4261 attttgcgat atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa
     4321 cagccaaaca aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg
     4381 atcacatcta aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt
     4441 ccgattgcac gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg
     4501 tgttcttttg cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg
     4561 actggcagcc aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga
     4621 atacccgctg ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg
     4681 acctcttttg tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc
     4741 ccaccgactt tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca
     4801 ggatgatcgg cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca
     4861 tggtctccat cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt
     4921 gcctctgcaa attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca
     4981 cccttttatc ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct
     5041 ttccattgtg tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag
     5101 gtcataaaat taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct
     5161 aaaacatcat tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact
     5221 agtacacctg cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc
     5281 tttgccagta ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata
     5341 ccaacaccat actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt
     5401 acagttgctg ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc
     5461 agttctttca actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt
     5521 acaaggacag tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg
     5581 atatcatcat ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt
     5641 gccatatcga agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt
     5701 ccactatcgg cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca
     5761 tcaccaagca ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca
     5821 tgtccgcttc ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca
     5881 acacgggaag attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc
     5941 atttcttcga caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt
     6001 ctttccggca ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt
     6061 gatggctgct gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg
     6121 ctgctcttct tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc
     6181 cgcaaaaaca aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat
     6241 cgggtctgat ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga
     6301 tttttgcatc tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat
     6361 aagcaaaata ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg
     6421 tttcgctttc attaatgaac aattcaagta tataataaat ttctaatttt gggaaaatat
     6481 gtgtagtgct tcttgaagtt tgttatactt taattaaaag agaggagtga tcgaatgatt
     6541 acaacaacaa cgaacagtgt agagggaaaa gccgtagtag cttataaagg gatcgttttt
     6601 ggtgaggtca ttactgggat ccatgcattt aaagacttgg aggcggggtt gcgaaatatc
     6661 tttggcggcc gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga agaagcactt
     6721 gccgaaatgg ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg
     6781 gactatgaag tattaggcag cgacaatggc atgctgatgg tcacatgtag tggaacagct
     6841 gtctccacga gataagtagg ggacatctgt tttaataact aaagagagat gggacatgta
     6901 gtggcactca agaattagat ttttcagtct aacttcgagg tactctacct tcccatcctc
     6961 tcttttttat ttctttttgc ggatgattcc gtaagctgct gcgactaaca cacccgcccc
     7021 tgctaaggaa taactgacag aagctgtttt ttcatttgtt tttggcaaag tctgacttgt
     7081 tgatttggat ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag aggtcactga
     7141 tttttctcct gccgaatgca aaatatcttt tattcgttgg aaactagctg ctaagtcagc
     7201 tttttttgct tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc
     7261 ttgactggcc gctgtgatat ccgcttgagt ttgacttagc tttccagaag ctgtttgagc
     7321 aacctgattt aattgctgat tgaattcttt tgcttgttgg atcttcccgt caagatctgt
     7381 ttggacagct tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc
     7441 atttcccact ttatttttaa gatcagctaa atcttgatcg attgctgctt ggtcttcttt
     7501 caatttgttt tttgcagctt ctaatgcttc ttcgagttct tttcttttat tttccaagtc
     7561 ttgcaaatga tcgatttggt cagaagaggt agaactattt attgatgtat cttctgaaga
     7621 tgaaatcgtt tctcctgaat tatctgtcgg tacattggaa gtcatttctg tttccgaggc
     7681 accgttttca gcaccagaag agcttatggt tgttgaaggt tctgtttgag caggttcaga
     7741 agtttccgca aaagctgtct gtcccaataa gaaaatactg gaaacagcaa ttggccagat
     7801 taacggcttc attctttttt tcatcacttt tcccatcctt actacttttt aactaatttc
     7861 ttattataag caaaaatgtt tggaagaacc ataaacacaa caggctgttt gtctaaaaaa
     7921 cggtgcatct tttatttttt tgtacgttcc gggcttttcc atgtcccgct tccataacct
     7981 aaaatcgttt tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag
     8041 aacaaaagca tatatagtgg tgcgaaaaga aggtatttca attttcttcg tcgatcatct
     8101 acaatcagcg aagctagcaa ctggaaaaat cctgcgacca tttcaaagca gatgaacaca
     8161 aaactgaatg taaaagtaat ataaatctgt tcgtagtttc cttgatatac ataatgcccg
     8221 attaatccag caaaaagaac tacggaaata caaaagaata ttgaccagac aatactcaat
     8281 gtttgatcaa tgaagatgat cgttcttcca atatgtttaa aaggatggag catcactttt
     8341 ttgaaattcg tcagccagac ttctgttcca ccttttgccc aacgttttct gtgtcgatat
     8401 agcatcttca acgtcacagg tacttccatg aaaaagataa tctcaggagc aaaaacagat
     8461 aaccagtcat ttaactgatg atcccaagca atactgatgt cttctgtcgc tcgatcttgt
     8521 ctgaatccgc ccacatcgat caaggcttct tttcgataca tcgtatttgc tccactgtac
     8581 gcataaagac caccgaaaac agctgactgt gttcgtttaa tgatgcccac gatacttgag
     8641 aattcaaccg tttgagattt tgcaatcaat ttggtacgat tttggacatc catatttgca
     8701 gtgaccgcag caatatgtct tgcaccaggg cgaatgaaat aattcacata cctaatcagt
     8761 gcatctggct ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag
     8821 gcaagtccga tattaaacgc gtgggctttt cctttatttt tttcgatccg aaccacacga
     8881 agattagcgt attttttcat cagtctagct aatatttctg gtgtttggtc cgttgaacca
     8941 tcgtctgtca ctaatacctc atagttgtga taattcaatt tcgtcatcaa atactcgatt
     9001 gtatattcga tcacaatttc ttcattatgg gctggcacca tgatcgtgat gaacggttcg
     9061 acactcaagg gaacatctac ccattctttt tgcttatgtt tgtataaaaa cacataacac
     9121 cagacaccaa taaaccatac aaatccgcct aaaatcggat aagaaacgag aaaaaaatac
     9181 ataatagaca aacaagtatc taagaataaa ttacctgtat gggtgattga gatcatttca
     9241 gttccacccc attttcttta tacaaatctg caatgaatgt cgtatctaga tttttctctt
     9301 cggagacaga ataaaaacgg accgtttctc tctcttcttt tgttccaaaa cgttccgtat
     9361 acacttcttc aagtaattct tgtctttttt tcaatttttc atcttcatat tcaaaagatt
     9421 tatttacttt tttgtataga tttcgattgt tccacctagt caatacaaat gaggtaatca
     9481 ggaaactgaa aaaaatcaat acaaaaaagc cgattaagaa atacagcatt ttcttttctg
     9541 ataaaaaagc gtacaaggaa aaatccttgg ctctttctgg tctcgttaat gggaaaaaga
     9601 cccatataaa aggaagaaca acgatcatcc atcctaaaag agccactaac gtttgccaaa
     9661 tctttaacca gtaattacct tttgaaaaat acttatcttt caccaaaatt gatgcaactt
     9721 cttctcttgt tacttttttc atatcctcac ctcacgtttt tttgttacga cgtaggagtt
     9781 taccatacat ttcatttctt ttcacataaa gtgttcctaa atttttccta agaaaaagaa
     9841 caaaacattt ctcatcagca aatgaaaaat attttgttct ttttatcagt tgttcttttt
     9901 attttttatc tcttaattat gttgctcttt ccaatacgac aatgcttttc attcctctag
     9961 atatcgccgt gtacatttgt ctgcgtcttc tttgctcatc tccatcgttt tcaaatggat
    10021 ataagattac atgatcaaat tccagtcctt ttgctagact caaaggaatg atttggcagt
    10081 tttctaatcc atatgaattt tttttaatcg attcttgtag cgtttgggtt tcctcttctg
    10141 tttgggtgat aactgccgct ttttctcctt ttagagaaac caaaatctca gctagtattt
    10201 tcaacaattc ttcagaggat cgaaaagagc ggtattcggg ttcttttcct tgcggtcgga
    10261 tcgatacgat gtcgatcttc tcttgatcca cagcataggt tttgaaaagc tctgtgattg
    10321 ctccgctaga acggtaacta tttctaagat cataccgatg gatcggcaga tggtagtcgt
    10381 cgaaacaacg cataatatca gcaaaagtag tggaagaatt aaagattgcc tgattctcgt
    10441 cacctactaa agtaaagtga gtttttggaa ataattcgat caatagacct aattgtgcca
    10501 gggtataatc ttgtacttca tcaatcaata gatagcgaaa tgctggtaca tttacctttt
    10561 caacaagtaa atgacgaata gctaatataa tgactgcttc atcaacagta cgtgtgggtt
    10621 gccaagcata agctgagcca tatcgttttt cataaattct ttcaaacaac tgatgctcat
    10681 ccacccatgc catttcttcg atttgtctgg ttatttttcg atattttttt tgtaaaagtt
    10741 tccttgcata agctacaata ctttgttccg aatcatcaga aatcaattta ccgaataatt
    10801 ccaactgcct atcttctgtc aacgagagta cctgattctg cacagcagga ctttttgctt
    10861 gtttcaaaag atgattcttc cattcgctca tcagtgcctt tttcgttgct tgaagcctat
    10921 cgatcaattt aggatgagca ggaactttct catatatttt ttcgatcgtt tttttagaaa
    10981 ggactgtttt tccattttta gtaagtccac ggataaaatt cggatgatct aaaaacaaag
    11041 gatctgattg ttttagatta tcgataaact ttttgctacg caaacgttct gtctgctccg
    11101 atacattttc tcccgtgata cgtttgaaat aagcctcttc accttctatt tcctctgctg
    11161 aaagctgtga aaccagctgt atgatggtta gattcagtgg atttctttca cctaatgatg
    11221 gtaggacatt cgaaatatag tcgataaacc gatgattagg tgatagaatc agcaagtcat
    11281 cggatgtcat cgtatctcga tataaataga gcagatacgc aatacgctgc atgatagcag
    11341 aggttttacc acttcctgct acgccattta ccaagatatt ttgacttttc acatcccgaa
    11401 tgattgtgtt ttgttccgat tggatcgtag cagtgatagc cttcatttca ttggtctcat
    11461 cttggcctaa gacttctaat aatacgtcat cttgaatagc tacagacgta tcaaaaaact
    11521 tgaccaaacg atctctttcc aaaagaaatt gtcgtctttc tttaataacc gtatcgattt
    11581 tattttgacg aaccgtataa gaagatggac caatcatgtt attgtaaaaa agttcggcaa
    11641 ttggtgaacg ccaatcataa acaagatttt catctgcatc atctgtgaaa tttgctgtac
    11701 cgatataaaa ggattctttt ggctcattat ccagaaagtc tacaacgact tttccaaagt
    11761 agggactttt caataaacgt tcgatctttg ctagctgatt ttctgcgttt ttctgtttga
    11821 tattcatctg atcgatttct ctatttttca tttcaatcat cgaaaaagta tccaggttat
    11881 ctaagtaact gtcaaaattc aatcgaagct cgccagccat ttctttcaac gactggtttc
    11941 cttctatttt tgccgtatcc atcgattccc gcagttcgtt ttttttatca attagctttt
    12001 gatacacgtt cttcagatat gcttcttcta actgtttttg tgtgttgacc atacacattc
    12061 tcctcttttt ttaaagtggt ggaaccgatt agtttacctc ttttaagagt cgaatgcaag
    12121 ctttttaacg agtatcattt gttaggatat atcagttctt caggtcggac agtttgatat
    12181 aaagaaaatg caccatcaag gttttttacg tggtagccag actgcttcaa gattctttcc
    12241 gctagatagc tacgtaatcc gctatgacag ctgacgatat attcttgttg gctgtctaat
    12301 tcatttaatc ggtcccgaag ttcattcaaa gggatatgtt tggcttcctt gaatttgccc
    12361 ttttccaatt cttctgctgt tcgcacatct aataaaattt ttccttttgc tagttctgtc
    12421 ggcaattcat gccattgtac ggtttcacta agcccttctg caatgttcat tgctgcatat
    12481 cctagcatgt ttactggatc tttggctgag ccaaatggcg gtgcatacgt caattctaat
    12541 tccggtaaat cgaagatcgt caaatgccct ttgattgctg ttgctaaaat atcgatccgt
    12601 ttatccacac cttttgcccc aacaccttgt gcaccataaa tctcgccagt ctctggatgg
    12661 aagatcaact tcagtaaaat atctgttgct tctggataat agcttgcatg atcttttcct
    12721 gatacatgaa tgacttcaaa agacagttcg ttttctttgg ctgttctttc acttaaaccg
    12781 gtggatgcgg cagtcattcc aaacgttcgt ataattgctg ttccaatacc gccttgattt
    12841 cttcgagaaa ttccggaaat cgtgtccgca acttgtctac cttgacgatt ggctggtgaa
    12901 gcaagagaaa taagtgcatc ttttccagtg atttgctgtt ttacaacgat agcatccccc
    12961 accgcataaa tatcaggaat attcgtttga taacgttcgt ctactaggat acccccgcgt
    13021 atgcctaatt cgattccagc ggtcactgct agctggttat caggacgaac accaatggat
    13081 aaaataacta aatcagcagg gatggtacgc ccatcatcca gtcgcaatcg tttcccgtgg
    13141 tcttcaaatc cagcgacagc atgagaagta atcacttcta ctccttgatg agacaattct
    13201 ttttctataa aagctgccat ttcttcatcc agtgttggaa gaatatgtgg tgcttgttcg
    13261 atcaccatga cttctaaacc tcttcttttt aggttttctg ccatttccaa tccgatgaat
    13321 cctgcaccaa cgatcacggc acgctttggc tgtttttcta atgcatgcat cactttatcg
    13381 atatctggta catttctaat agaaaaaaca ttgtcggctt ctgttatccc tggaatcgat
    13441 ggaacaactg gttttgcacc aggagataaa accaatgcat cgtactgttc aatgtgtttt
    13501 tgaccctcat gtacgacttc taccgttttg ttttcgggaa agatggctgt cacttcatga
    13561 tttggacgca catctaaaca aaaacgagcg gataatgatt ctggcgtttg gacaagaaga
    13621 ttttcccgtt cactgatttc tcccgaaaga taataaggca agccgcaatt tgcaaaagaa
    13681 acatacggtc ctttttcaaa aacaacgatt tctgcatctt ccattaatcg gcgaagccgt
    13741 gtagcagcag acattccacc tgctacacct ccgacaataa cgattttcat cttatttccc
    13801 tcctactgtt cgatcaaacc attgattcat gccaccacgt acattcacta catcataacc
    13861 agatttcttt aattctttag ctgcttgttt actcctcatc cctgattgac aaatcacata
    13921 gacggtcttt tctttgtctc catcatatct gtttatactt tgtaatggaa catttttagc
    13981 tccttttata tgcccgcccc ggtattctgc aggtgttcta acatcaagta acgctggatt
    14041 ttccaaaagc ttttccttta attctgggac tgttatcgat tttattgatt gaaatagcaa
    14101 attcattttc tgcacctcat ttacattttt ataaatacct catggggtat attaaacagg
    14161 agctttatta atatgtcaac cagaatgact cttcattttt ttcactactt tttatatttg
    14221 cataaaatac cgataagggg tattatctaa ttataaaaat agaggagaat gtgttatgga
    14281 gattgataat aaaaatatcc tgaaccgttt gaaacgtaca gagggacaaa ttcgtggtat
    14341 ccaaaaaatg atcgaagacg aaaaagaatg tatggatgtc atcactcaat taagcgctgt
    14401 tcgttctagt attgatcgcg taatgggcat gatcgttgca gataatctga aaaattgctt
    14461 tgagaaacct gatagtaatc cagaagaaca agcggctaaa ctagaacaag cgatcaagat
    14521 gattattaaa aagtaaagga atgatttcaa tgaaagtcgt tttccatatt gatgaattag
    14581 aaaaatggtc agaaacaggg aaaaacgtaa aaaacctgat aaaagcttct ccagaaacga
    14641 cgattgtcgt atctgtaaat ggcatcgcga ttaccggtta tctagactct gccaatgcag
    14701 aattcttaga tttgcaagga gtcgtttttc acgcttgcgc caatgctatg cgtgctaatc
    14761 atatttcaga aagttcttta cctgaacaag taatcgttgt accagcaggt gtccttgatt
    14821 tagttgaact gcaatcccaa ggttatgctt atatcaagcc ttagtcagta gaggttgcga
    14881 cagaagtgat cagcttcaag aaatttcggc ttatttttcg aagaagctac ttctggaaaa
    14941 ccgtgaatca aaggttgtga aaacgctgtc ctgcatcaag taataagaac agccaaacaa
    15001 aaatagcttc tcatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa
    15061 caccgtttaa ggaagaggtt gtgaaatcag cggtttgacc taagtattag agaaaaaaac
    15121 gggaaaatag ctcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg
    15181 aacaccgttt aaggaagaga ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt
    15241 ttgtgaaaat ggatttctac atttttgcat aaatttggct taataccgag aaactggctt
    15301 ttgaataccg tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagaaa
    15361 caaacggaaa aatagctgct catattttgc cgttttttga gctaataccg aaggtcagct
    15421 gattgaacac cgtttaccaa gaggttgtga gcctaccatt taactccgaa tcacaagaaa
    15481 taatttcaaa aacaacttct cctgtttttg gttcggttgg cttattatcg agaagtagct
    15541 actgggagtc tatttatcaa aaaaaggaat gcgacatagg tcacacccct tttttatttt
    15601 ctttgaaaca tctttcggta ctcgacagca tacaagacaa aagattgtaa aagaccgtaa
    15661 gtgatgacgg caaaaatgac tctcacccac agcgttggta attctgggaa aagcagcaaa
    15721 aggatcatgc caacatagat gacagcagtc gctactttgc cataccactt cgctccactt
    15781 aatttgatat ttttagatag cccgattaat ccagctacaa acataaagcc ttcttttatc
    15841 aagaatagac caaacaacag ccaaagccac ggtcgatacc atgccataga caaaatcaga
    15901 aaaagttgtg tcaatttatc tgcaaacgga tcaagcacct tccctaagtc tgttacctga
    15961 ttatatctcc gagcaatgac cccgtctaga taatcagaca aacctgagac aacaatgacc
    16021 gctcctgcta tataaaatga ctgtctcagc acaaaataaa gataaatcgg cagcaaaaaa
    16081 atacggaata tcgataaaag attcggaatc gtttgtattt ctttacgcca gtctgtgctt
    16141 aatttcattt tatcgcccta cctcctcagc actcttttct ttttatttta gcttacacgg
    16201 ggaataaaac caatgatttc actgttttta tttttcttta cagacactga tattatgagc
    16261 taatttgaga taatcacttg ctgttttcat cgtttactct tctttcttcc tgtcgtttgc
    16321 gcatttcttt tactagtact ttatgagctt tttttgatcc tcgctctagg tcacgctgcc
    16381 gtttacgtgt tttataatcg ataaacagac tgtctaaatc gtaacctgtc gggtacagat
    16441 cttctgctga acgcaccaga cgaacacgct gacgaggcac gtggattatt tttttgtcta
    16501 aatatacatc aatcgtatct tcacctatat cttcataaac taacgcttct ttttggtatt
    16561 cattgacaaa tatccgatca ccttttgcaa agagctgacg ttctttttta ttctccttcc
    16621 tatttgtaac tgctgtctta ggaagaaatt cttttttctg tgtccgatag gctttttccg
    16681 ccaagtaacg ctctgcttga tggatcaatt tcatcgacat atccatttta tgagcaatcc
    16741 acaaagcttg gctttctcct gtttcttcca acccgcaact gatatttagg acgaagtgct
    16801 tcccgatcga aagccatagc agccggcaca aaatcttcat gatctccggc gaatttcttg
    16861 atttctccat aatgggtcgt ggcaatgatc aatgcccctt gctcatacat agattccatg
    16921 attgcgatcg ctagtgcagc cccttcattt ggctctgttc cactgccaat ctcatctaaa
    16981 agtaccaacg tattcctttt tatctttttc aaaatagcag cgatgttttg catatgacca
    17041 gaaaatgtac tcaacgcatt ttctaaattt tggtggtcac cgacatcgac gaaaatctca
    17101 tcaaagacag caagctctgt cccttctttt gccggaactt gcataccgaa catcgccatc
    17161 aaagtcaata aaccgactgt cttcaacacg actgttttgc ctccggcatt tgctccggtg
    17221 ataatcaagc ctcgatagtc tttaccgatt tcaacatcca atggtaccgc atgctcaggt
    17281 aaaaatggat gtctgccttg cttgataatg atatgttccg atttattgat tctaggtgtg
    17341 cttccattga tttctcggct aaattttgcc cgagcaaaga tgatatctaa caccgtgatc
    17401 gtttcgatca ataaatcgat cacttcttcg ttttctgcga tagcaccagt caatgccgcc
    17461 aggacttgat attcttcact gatttcttca gcttttaaca actgatagtg ttcattcaat
    17521 ttagaaatca cagttggttc aataaaaacc gtcgttcttt tatttgattc atcgataatc
    17581 gttccatcta ttttattttt atagctggct ttgataggaa tcgtatagta ttcgcctttt
    17641 tgaacaatca ttgcttcttg gatcatttct ttgtttttcg gatgacgcaa gaatttcagt
    17701 aattttgact ggatttcttt ttcgattagt tgtaattgtt tgcgtacttt acgcaaatta
    17761 cgtgacgcgt cgtcactgac tttattattt tttattttct ggtcaatcaa ctcttctaca
    17821 tttatcaaat caggaagatg tttgctataa gcaaacaaca agggtgtctg gtattgattc
    17881 ttatcaaaaa actttgtgat catccgactg cttctaagaa aatccgcata ttcgatcaaa
    17941 tcggttggct gcagaatcaa cccttttttg acttgttctg tcagtgcatc gatcctcggc
    18001 aaccccataa atggaacatg ctgactgctt tctaagatca agcgtgcttc tcttgtttct
    18061 tcttgacgat ccaagacagt ttgtaggttt gtcgagaccg tcatatcgct gattcgtttt
    18121 ttgctatagt ttccaattgc tcttgcttgg atttcctttt tgatccgatc aaattgtaat
    18181 tgactaatca tttcgttgtc catattctct ccttttcttg aagagatacc tgtcccaaaa
    18241 taaataaggg cctagagata aataaaagga tagaacacag cttaaaaaag tcatgtccta
    18301 tccttattag aatacaaaga tattgttttc ctgacaggta ccgtttcata agcacaccaa
    18361 ataaactttg tttattggct attcgactta ctgattagat accagctaca ctcacaaaac
    18421 ataccccatt cttctaggtc ttatttattt tccacttaac tatagcaaga cacattttgg
    18481 ttgtcaaact ttagtatgtt ttatagattt acaccattaa tgaatagaca cctaccgcga
    18541 taattgctcc gatcattgga cctgcgattg gtacccatga atatgcccaa tctgaagttc
    18601 ctttattagc tattggcaat accgcatgtg ctaatcgagg accaaagtcc cgagcagggt
    18661 tgattgcata tcctgttggc cctcctaatg aaagaccgat ggctaagatc aatccaccta
    18721 cagcgaatac gttcgttcca ggtgcaaatt cgttttgtcc aaaagctagc aacccaagaa
    18781 ctaatacaaa tgtaccaatt aattctgtga tcacatttgc tggataattc cgtatagcag
    18841 gacctgttgc aaacgttcct aaaatcgcgc tttcatcttt cgttgcattc caatgtggca
    18901 aataagaaag ccaaacaaga atggcccctg caaaagctcc taaaacttgc gcaatgatga
    18961 aaggaacgac aagattccaa ctaattgccc ctgtcatcgc catagctaga gagactgcag
    19021 gattcaaatg agctgggccc atgaatccag aaatatagac agccattgtt acagcgagtc
    19081 cccaaccaaa tgcgatgaca atccagccag aagcctgtgc tttacttttg tttaaattca
    19141 cagccgcaca cacaccatct ccaagcaaaa ctaggatcag tgtcccaata aactccccca
    19201 tgatttgagt catatcactg ttcatttatt tctccccctt tagttcccgc agatctgatt
    19261 ctgcaatcaa tgcttctaaa cgtttagtct gttgcgcctt ctcctcttct gtccattcaa
    19321 aatatccagc aattgcatcg atcacaggtt gcttgatctc atcaagctga tctcgttcaa
    19381 acaacagatg atttgtacga cgaatgagat aatcgcctgg ggccaaaacc atttcttctt
    19441 ctaaaccata acgtaaccga gctgactcag cgagactcaa accaggataa ggtgccattt
    19501 cttttgctaa ttcaaagata cgtcgggcat tagtcccgta aaaatcagcg atataagtag
    19561 catcttcttc ctctaaaccg gctgctactc cttcttttgc taattctgtc actgtttctt
    19621 ctaatttagt tggatcgaag tttccacctg aaatctgata ttttttagag tcgatttctt
    19681 tcgtctctat tccgtattct tcttttaata attggcgtat caatcgcaaa gctccttctg
    19741 ccatcttacg gtaatcggtg attttcccac cagataaagt cagcaaacca tctggttctc
    19801 tttctaaaga actacctctg gaaatcgtag aaggtgcttt ttcatcacgg ctgttttcca
    19861 aatggttcaa cacgtcttct acttcagcac gagaaacttt gttttcctta tattcactta
    19921 ctgtatcgac aactttattg aagctcttgt ctgaaatcga tccattatct ccaccattgt
    19981 aatcagaacc agaattacca atcaacagtg gacgaagtcc tgcccagctt gcttcgatat
    20041 cattcaatgt gatatttgct tctggatagc gatgattgat cacatccaat agataatcta
    20101 catcttcttg tgtgactttc ggatccgtaa aatctccttg gtaatccgta tctgtcgtac
    20161 caaagtaagt cttgttttct cttggaatag caaaaaccat ccgcccatct tgctttcctg
    20221 tatcgaagta tgtgggttgc ggtacaggca gttttttcgc atctacaact aaatggatcc
    20281 ctttagttgg acgcatttta ggagagactg gacgcgtaaa attcaagttt cgtactttat
    20341 ctacccaagg accactcgta ttgatcacta attttgcttt gatttcgatc acttcatctg
    20401 tcagcaggtc acgagctttg acgccaacga tttgatcgcc atcatataaa aagccaaccg
    20461 ctttcatttt acttactaga taagccccat cttcggcagc ctttttgata ttatcaatca
    20521 ctaaacgggc atcattgttg cggaaatcta gatagacacc cgcgcctttt agcccttctt
    20581 ttttcaaaaa cggttctctt tccaatactt cttctggtgt cagcgtataa ttctcatatt
    20641 tggttcctgt cacatttgcc agtttgtcat aaaggtccat tgctactttg accgagaaca
    20701 tattgaaggt tgttgctcct tcgccctcat agattggcaa aagcattgga tctggttttg
    20761 ggatgtgtgg ggcaatactt tgtacgactg cacgttcacc aactgtgtca gctactactt
    20821 ccacatcaaa tgtcttcaga taacgaatac cgccatgaac aagtttggtc gagcgagagg
    20881 aagtcccttc tgcaaaatcc tgcatttcga tcaatcctgt tttgattcct gatgctgctg
    20941 actggactgc cacaccggca ccagtgattc cgccaccgat gatcaacaga tccaattctt
    21001 cttgctgcat tttttgaatg ctatcttgtc ttgtcttgtt tgaaaacata tggatttcct
    21061 ccttattcaa atgcttgggt agcttttact gcttttttcc aaccgttgta aagtttttct
    21121 cggcgctctt cctccatttg aacttcaaat agcttgccag cttcataaaa ttcgcggatc
    21181 tcgtctgtat ctttccaata accgacagct aatcctgcta aaaatgctgc ccctagcgcc
    21241 gttgtttcca agttttctgc tcgttggatc ggtacattca agatatctgt ttggaattgc
    21301 atcagatatt cattgtttgc tgcaccgcca tctactttca atacaggaat cttgattcca
    21361 gtgtcatctt gcatcgtatc gatgatatct ctcacttgat aagcaatcga ctgtaaagta
    21421 gctttaatga tatcttcttt tgtcgtacca cgtgtcaagc caaacatagc ccctcgtgct
    21481 gcctgatccc aataaggagc acctagccca acgaatgcag gaacaacata aacctcatct
    21541 tcgcttgtag agcgtttcgc tgcgtcttct gaatcgctag ctttttgcaa catttgaagt
    21601 ccatctctca gccattgaat cgaagaacca gccacaaaaa tacttccttc taaagcatag
    21661 taaacctttc catttatgcc gtaaccaatt gtcgtcaata aattattttt ggataattgc
    21721 ggtttttcac cggtattcat gacaataaat gaacctgtac cataagtatt cttgaccatg
    21781 cctggttcga atgccatttg tccgaataat gcggcttgct ggtctcctgc catccctgaa
    21841 attggtgctt cgcttccata aaaatgataa ccttgcgtat aaccataaac ttctgagtta
    21901 gaagtgactt ttggtaacat cactcgagga atatttagta aatctaaaat ctcctgatcc
    21961 caatctagct gatggatatt gaacaacatc gtccgactgg cattcgagta atcagtaaca
    22021 tgtgcttgtc catcggtcag tttccatact aaccaagaat cgatcgttcc aaacatcaat
    22081 tcgccttttt cagctcgttc ttgcgcgcct tctacatgat caagaatcca gcgtatcttc
    22141 gttgctgaga aataggcatc gatcactaac cctgtttttt cgtggaaaaa ttcttgatag
    22201 ccttcatttt ttaagttatc tgcaattcca gctgactgtc tggattgcca aacaactgca
    22261 tgatagattg gacgtcctgt atctttttcc catattactg ttgtttcacg ctggttcgtt
    22321 atgccaatcc cagcaatctg atttggtttg atccctgatt cgatgaacgc tccggcaata
    22381 actgactgta cagaattcca gatttcattt gcatcatgtt ccacccaact ttcttgcggg
    22441 aaatattgcg tgaactcttt ttgagaactg ccgatgtgtc ttcctttctt gtcaaagata
    22501 attgctcgtg agctagttgt accttggtca atcgccatga tatacgttga ttctgtcatt
    22561 gtgattccct ccattatatg caaacgcttt ctttaactac tcttatcata ctactgtttt
    22621 aaaaaaagaa actgtcaaaa ttttattctt tttttctttt tttgacagtt ttttattatg
    22681 aagctatttt ttaatcaatt cttttctttt ttcagcgtct ctattcttct tttcaatcga
    22741 ataatgtcgt aaggtgacgc aaattctgac agataatatt cacactgttt tttgtttctc
    22801 tggcaaaatg tgatcaccaa atcatattct ttaccttctt cataacttcc aatctcaata
    22861 ccgccgattc ccctcagttc tctgatcaga tattgttgaa aagcaatacg ataaatcggc
    22921 agactttcta aatcgattcc aatcactact ggttttgcaa tataaaaatc gatcatcaaa
    22981 agaacattgg catactggat catcaggtaa ctaagctcac tatcttcagc tcttttcctc
    23041 ggcaattgct gcaccgctgt ttcctgtaat ttcgccagta actgtgttaa ggaatgcccg
    23101 agcattttct gctgctgttc caaaagccga tcacgctcat agacttcgat ctttccaaca
    23161 aagaaaaacc attcattgtt gatctgggaa atttggtagc ctactgcctt ttcttctttg
    23221 attttcaaac gacgaggacg ataatgccat aacatcgttt cccgaatgta agtatctaaa
    23281 acagcggttg gtaacttttt gctccgtgtc aaatcatact gataaaatat ttcttcgtct
    23341 actatcgaaa aagaaacaaa aaagctgtaa aacatcattg tttcgtatac attcagttct
    23401 gctgctgttc gactaagatg catcacgatg attggatcga ttgcttgata aagccggtcc
    23461 gactgataga gtaatttctt ttctttcaaa gttgcgtacg tacttttttc attcaatagt
    23521 ctttttttcg taatcccgag ccagcaagca atctgtgctt ctgctgatgc cgtaaacgtt
    23581 gtttctaata cacggtccaa tcctagaatg aacggtcttt tttctggtgt attttgcaaa
    23641 aaaagcggtc gctgatcttc cgggatatat tggaatagtt cataataaaa atatcgtatc
    23701 tgtagctctt ctccgtataa ttgaccgttt ttgatttgta atccaaattc ctctaagagc
    23761 tggttcaatt ctttgatctt tcgaaaaaca gaagactcac tgatattaaa cgctgtcgct
    23821 aatcgaacga tggatacttg ctgatgttct aataaataga cgagcatctt aaacttcaag
    23881 ctttcctgca ataaaaacaa tagaattttt tccaaactta catcttcgtc caacacaagc
    23941 agcgcctgat actcatttcg ctgtacttct actttttttt ccatcattcg gccaatcaat
    24001 tcaatatctt ccaaatattg atcaaacgag gcttttgaca gttggacatg ctctctcaaa
    24061 ccattgatag aggctgttcc cccttgcaga acaaggtttc gcaaaatcag gatctgtaac
    24121 tgctcttttc tttctaataa tgcttcaatc ttcataggca gtattcgcca cctttgacca
    24181 caccatatct ggcacacggt attctggctg gaaaaatagc tcatgttctt ctaactgttc
    24241 aaacgtccat ccttcttcta ctgcaattgt cagttggtca agaaggtgga acatctcttt
    24301 tttgctttgg agctgtgctc caagaatctt tttttcagaa tcaaatagaa atttgagttt
    24361 aactttgctt tgatttattg ctgtaagctt gtagtctacg tatcctgttt caattttttg
    24421 gggataaaaa atgctctctt cttctgtcaa acctacgctt cctaagaaat agccaaacca
    24481 ctgattgccg actggacgat aggttccctg atcctttgtc tgtgagccac taattgtctt
    24541 gctgacattt cgtgctgtac ggacagcgtt atttactaga gaaacataca ttttttcatt
    24601 tgttggacga aaagttactt ggatcgcatc gccaatcgca taaacgtctt tttgagacgt
    24661 ttgcaagtag tcatctaccc agatcgttcc atcatcattc aatgttaatt gttcttccca
    24721 catctgattg tcgggacgag aatgattagc cagcaaaaca agatcatagt tctcctttct
    24781 tttttccgtc tctacaatag cggtttcatt ttcggtcaaa gacacaactt gttcttctaa
    24841 aaaaagtgaa agcccttgat ttttcatttc ctttactaac ggttctgtca tttcaggatc
    24901 tagataacga aaaagaattg actttcggct ttcgtaaaga tggatttgtt tttttcttga
    24961 tgccaaccct tcagctattt cgattcctgc ttgtcctgca ccaataattg caattttctt
    25021 tgcattcatg atcttttgct gaagagccgt tacgttttgt atctttttaa ctgggtgaat
    25081 aagcgttgat tctgcagaaa tatttcgaaa cttttgattt gaaccagtcg ctagaatcaa
    25141 acgatcaaaa tccaattttt ttcctcctgc taaaacgact tttttttctt tcaatcctat
    25201 tactgtcttt tctgtgtaaa catggattcg atagcggtca gtcagttctt ctttcgtgat
    25261 ccaagaaagt tgtttttcac tggtaacctt tccttttaat atcaaattga acccgctggg
    25321 aatccatcct aacttcgatt gcttttcaat caaatatatt tcacttgttg gcatcaattg
    25381 gcgaagataa atagccgcat gaatccctgc aaacgacccg ccgacaatta caatgttcat
    25441 ctgcattcct ctttcttata aggtgttcta ctgctaactt ctcaatcaga aaacacagta
    25501 ttcataactc gctttcaacc ttatgataca tagcgatgca gcagaatcaa ccctctccat
    25561 cataccgtgt tctaggtctg cttctcaata atgtactagt ggacctaatt gaagagtatc
    25621 atttctgaag agatgtttca aaaaatgtac tcttataaaa gaaaaaaact cgccttttac
    25681 gtacctctgt ccaaatagga aacagaaaag aacgaaaaga cgagttttag cgtgtgtaat
    25741 gtaatagtta gaatagacac atcatttatt tttaattctt taatgcttca acaactgctt
    25801 cgcctaattg atgagcagat tgaggatttt gccctgtaag aagccgccca tcaatttgaa
    25861 ccacttctga aaaagctgct ccagactgat agtttgctcc tttttctttc aaagcatctt
    25921 ctgctaagaa tggtacttca ttcgttgtac catttgcttc ctcttcttca ttactgaacc
    25981 cagtcactgt acgtccagct aaaatagatt ttccagactc atctgtaact ggcaacaatc
    26041 ctacaacacc gtgacaaaca gctgagacca gcccttcatt tcgatagatt tgtgaagcga
    26101 ctttcccaag atctattgct tctggaaaat cccacattgt tccgtggcca ccagcataat
    26161 aaaccgctgc ataatcattt gcatccacat catcaggacg taaggtattt cccaaggccc
    26221 gatttctgaa atcttcatct tcataatagg cccaatcgat ttcatccata ttcaaaacac
    26281 tttgcggttc taaaggtaca tatccgcctt taggactgac aaaatctgca tcgatcccat
    26341 tttttactaa tacatcataa aaatgagtca gttcacttaa ccacaaacct gtaggacgct
    26401 cgatcttttc gtacttttct acatttgtta atacgatcaa tactttcatt ttcccatcct
    26461 ctttctataa aaattgcgtt attgataacg tgctttcaag aaattatata atgggcttgg
    26521 caaataaagc tctttatatg ctttttcaaa aggtttcgtt acaaattcat cctcacggtt
    26581 ttccaataat ttctctgccc agtcaggatc gacgatcatt tgctgtccaa ttgctgcaag
    26641 ttctgcatat ttcaatacct gctctacatc ttcgcgattg cggatcccac cgacaccaac
    26701 taaagggatc tttccttgaa tcgtgttatg gaccacttca agaatcgatt tatcgttgta
    26761 tttatcagaa cgagctattc gatcatacat atttaatgaa acatgcagat aatctagttt
    26821 tgactcacgt aatcgttcaa gaagccagat cgtgtcttca aaacggatac ctggtgtttc
    26881 atattcttct ggtgagaaac gataccctac gatgaatggt tttttagcat attgttcaac
    26941 cgctgagaaa accgttgtta ataattcttc aataaaacga taacgatttt ccaagctacc
    27001 gccccattga tcctctcgtc tattcgaatg cggagagaaa aattgttgga tcaaatacgt
    27061 atttgctcca tgcaactcga ttccatcaaa tcctgattca atcgctctac gtgtggcttt
    27121 gccaaatgct tggattgttt caacgatttc ttcttcactc attgctcgtg gtgtttccgc
    27181 atcaggacgt tttgctgcaa ctgcacttgc agatacaggc tgttctccag ataaagttgc
    27241 tcgacttgtc attcgtccac cgtggaaaat ctgaataatc gcttttgcgc cttctccttg
    27301 gattgctttc gctagctcac ttaatcgagg aatcatttca tcatgagcga tgctgagttc
    27361 acctggccag ccttttccga tatcttggac atttgcagca ccagtaataa cagcaccaag
    27421 acctttagaa cgttgtttat aataagtgat ctcatcttcg gtgatcactc cattgaaaaa
    27481 gctgagttgt gtcgtcatag gcgacagcat caatcggttc ttcaattgaa ctcctgaagg
    27541 taaagtaatt gattgatcaa attgtgacat tgtcattcct cccataattc gctctgatta
    27601 acctatcgag aatcatctat cattaaacca ctcgattagt tgtacatgca actaatttgc
    27661 ttaaaaaatt ttattttttt tgtaacttat ctgttgcatc attcattgtc tgggctaatt
    27721 ctttacttgc ttcatttccc aaaactgctt tgtatgactg atgaagttct tcccagctac
    27781 catcgacttc cttagcaaat tggcgtcctt gttctgtaag ataaactaat gacaagcgac
    27841 cttcgctaac tgttttgacg tatcctttat attctaattt ttcaacaaaa cgagcaatcg
    27901 ttgatggggc ggaatagagc atttgtgcaa tatctttttg tggtattcca tctttttctt
    27961 ttaatatcaa taaaataaaa ccataagttg gtgccagacc tgtccttcta aacgcttcat
    28021 cagctaattt attcaacatg cgatctagct ttttcacgct aaagaaaaga cattcatcta
    28081 acgctttttc aattggcttt tccatttcat cccctccatt ggcgtacata tcatagtatg
    28141 atttttattt tttgtcaaaa aacacggaat aatgtccgcc tcctctgtct agatttgaaa
    28201 aaaagaagta gccatcttca aagaatttcg gcttgttttc gacaaaactc cttctggaac
    28261 accgtgaatc agaggttgtg aaaaagtcgt ttagttttga gtatgaagaa aaattttgtg
    28321 aaaatggctt tccacatttt tgcataaatt tggcttaata ccgaaaaact ggcttttgaa
    28381 caccgtttat atcagaggtt gtgaaaaagc cgtcctgcat caagtaataa gaacagccaa
    28441 acaaaaatag ttcctcatat tttgtcgttt tttgagctaa tgccgatggt cagctgattg
    28501 tacaccgttt attgagaggt cgtgagcctg ccgttgaact ccgagtcaca aggagcaatt
    28561 tcaaaaaaca gcttctcatg ttttatgact atggtgactt gtgatcgagg agttggcagg
    28621 agaatactgt ttattcgtaa agaaggggct gtgacaagac ttttgtcaca gtcccttatt
    28681 tccttttatt ataggatttc gccatttgat tggatgactt gtctgtacca atcaaatgat
    28741 tttttcttta accgtttctt tgtgccattc ccttcgtcat cttgatcaac atagatatat
    28801 ccataacgtt ttgacatttg tgaagtagaa gcgctgacga tatcaatgca gccccacgac
    28861 gtataaccaa ataaatctac tccatctttg atagcttcct tcatctgctc aatatgctgc
    28921 ttcaaatagt caatgcggta atcgtcttga acggttccat cttcttctac tttgtctata
    28981 gctccgagtc cgttttcagc tatgaacagt ggtttttgat acctatcata taattgattc
    29041 aaagcaattc tcaaaccctt aggatcaatc tgccagcccc attcacttga tggaagatac
    29101 ggatttttaa ttcctccagt tattaagttt ccttggaccc cttcccaatt ttctggatga
    29161 gcactgactg cactagacat gtagtagcta aaagaaacaa aatcaacagg gtaggctgct
    29221 aagatttctt catcacctac ttctattttt aagttgacac ctttttcttc aaacattcgt
    29281 tgcgtatacg ctggataata tcctcttgct tgaacatcac taaagaacaa agaagttctt
    29341 tgctgctcaa ttgttttttg aatatcatcc ggatcacaag aatacggaac aagaagctga
    29401 tagctgatca tacaaccgac ttgtgaacct ttgataattt catgacaagc ttttgtagct
    29461 aaagcacttg ctataaactg atggtgtgca ccttggtatt tcgcttgttc caaatgggga
    29521 tggttttctt caattactcc tacgctaaca aaagggtgat gttttacaca gttgatctca
    29581 ttgaaagtca tccaatattt tactttattt ttatagcgtt tgaatactgt ttcagcgaaa
    29641 cgaacgtaaa actgaataag ctttcgatta ggccatccac catattctgt aaccaaatat
    29701 aacggcattt cataatggga aagagaaacg actggctcaa tcccatattt tagacattcg
    29761 tcaaacactt gatcataaaa aaataatccc gcttcattgg gtgtttcttc atcaccatga
    29821 ggaaaaattc ttgtccatgc gatagacatc cgatagcatt taaaacccat ttcagcaaat
    29881 aaacggatgt cttctttaaa gtgatgatag aaatcatttc cgtgtctttt aggataataa
    29941 tacttgctgt tgggatcctg cgccgctttt acttgctgat gcgtcatcat atgccattga
    30001 tctgtccatt tttctttagg taaatcgctg tcgaatgtat acacatcaga cacagataat
    30061 cctttttcat ctacatccca tgcaccttca cattgattgg ccgctgtcgc tccgccccat
    30121 aaaaatcctt ctggaaattt tttcatatcg atccccctaa acttgctagt tttcttgttt
    30181 gattattttt cttatgacta gtaatgatat ttttgaatct actactattt gtaacaataa
    30241 ccattgtggt cggatcatat ccctctttga ttaattcttg caaatctact tccatcaata
    30301 aatcgccttt acgtactttt tttccatcag atactgcact ttgaaagtat ttgccattta
    30361 aattcactgt atcaataccg atatggatca gtatctctgc tccttcttca ctgactaccc
    30421 caattgcatg tttcgttgga aaaactgttg aaacttcccc gtttacagaa ctgtagattt
    30481 ttccgtccgt tggaatgatc gcaaaccctt ctcctaaaga tccactagca aatgttggat
    30541 cgtttacttc agataaatca attatttcac ctgctgcagg agtacttaac actatatcct
    30601 ttgaggattc ttcttcgatt tcatccttgt agccgaaaag aaaagttagc atacaggaac
    30661 tgatcatcgc taagaaataa ctagcaacta gccaaacaaa tccttcacca atataaatcg
    30721 ggaaagacaa aatacttctt gtagcaactg ccaaagcacc ggctcctgcg gcaccagcta
    30781 ctccaccaaa tatagctcca catatacaag caatgtaaaa tggtcgttta tatttaagag
    30841 tcacaccata aacagcaggt tcagtgattc caaataatgc tgtgataaat gcagataaag
    30901 ataaactttt tactttctta tttttcgttt tcaaaaatac cccaagtact gctcctgctt
    30961 gcgcagctac ggcagtcata catgctggac ctagcgtatc acgaccgtag aaagaaagat
    31021 tgttggtcat tactggtaca agtccccaat gtaacccaaa aatcactaac acttgccaaa
    31081 atccgccgat aacagcacca gataaaacag ggctaaagtt ataaacagct aaatatccag
    31141 ctgcaatcag ctgacttgca taatctgcaa ttggacctac aactaataaa ataattggga
    31201 ccatcacgat gatacaaatg aatggtgtca ataaatttcg aatactttcg tgcaagcttt
    31261 tgcgaatctt aggctcaata taagaaagaa cccatacagc taaaattgct ggaataaccg
    31321 tacttgtata ccttgctaga atcacgggca tccctagaaa tcgcaaggtt atgctatcac
    31381 tatatgcact gacaatcgta ggataaacga gagctgctgc aataaccata gcgatgaaac
    31441 gatctgtatt gaactttttt gcagcagtat atgccaaaaa tataggcaag aaatagaaaa
    31501 atgcatcagc tgctgcattc aaaatgatat aagttcctga ttcagtagtt aataaattca
    31561 aactcgtaca taaggcgagt actcctttga tcaaaccact tcctgccaag acgcctaaag
    31621 tcggggcaaa aacagcggcg atcatgtcta taaaagagtt taaaatgcct tttttctcag
    31681 ttcctctctt tccactagat gcatcatctt ctagtttcat ttgtttggat aatacgtcat
    31741 acacttcatt tactgcattt cctactacga cttgaaacat tcctccagct tcgactacag
    31801 tgatcacacc ttctagttgc tctaccttct ccttatctgc cttcttatta tcttttaggg
    31861 taaagcgtaa ccttgttgca caatggtaaa cattgctgac attttcttcg cctccgacaa
    31921 atcggacaat atctttcgct aattgtgaat agtccatttt tacttccccc ttgttaatta
    31981 atgccgtagc aatccttctt acaagataag tatagttctt agaaaacggt ttctccattc
    32041 aattagtacc ccttctaata gcgaacgaaa ctgtacaata gtgtgtatgg tttacttagc
    32101 cgagcaaatc actcactgct tgaaagaaaa caagtatatc gtttctctta tagtcaaagg
    32161 agacaaaaaa aacacgcttg atcttccttt gctgatgtat aagtggtatc tcaagctgat
    32221 actctgtgat ttttgtttct acttggcttt tgataagaca gcacaacaaa gtctcggcat
    32281 ttaacggaag cataactagg tcagaactag gagagattag ttgctgaatg gaatctatct
    32341 ttttatggat ttttatttga ttttcttctt tgtagtaatg atcaagcagt tttgttttct
    32401 ttttttcttc aactatggtc agcttttcac gcaaaatctg ataaatctcc caagattcaa
    32461 actcatctag aacactaggt agataaatca aatcatccca ctcaaaaata agctcttgct
    32521 cagtagtcaa aattgcatca tattccgtta ttttgtttga ttgatttttg aaaagataca
    32581 ctggttctat ccggcattct ttgatccaca tgttcatact ttgttgaatc tgttttttta
    32641 gtgtattcaa aatactaatt ggctggttac taacaatcag tatctcccct tgttttcgaa
    32701 aaggattcaa cgcttcggca agatacaaaa caaaataagc gctttcagct aaattgggac
    32761 gtaattgaaa atgtattggg aagaaacgcc gaattaaata aatttctaaa ggataacggg
    32821 agatgatctt ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc ctcgttttca
    32881 tttgtttgat atgggttagc aaaaattctg tttcatggaa caataacgaa acaggaagat
    32941 ctaaaaaacg aataatcgtt tgctcaaatg ctcttagatt cttctctatt tctttgtttt
    33001 tttcatcatt aatcatcaaa taatttaact ctaatagtcg attttcaatt gcttgttttt
    33061 cttgattgga aaaagaatag ggtagttctg atgataactt ttttgtgagg gtctctacca
    33121 tcggaataaa taatgtatac ctgtttgttt tttctaaaag ttcttctctt cgaagaatgg
    33181 tcaaaacgag ataccgagaa aatcgtatga agtcctctcc tgaaacaata taattatatg
    33241 atataagaac ctcttttaat gtcttttgga cagtcggcat gatattggaa aaaattgtac
    33301 tgatttgttg aggaaacctt gcgtgttgga tcaccgtttc tgttccaaca agagataaga
    33361 aaattctttt tgaattttct tcagcaatga tttttagtcc ttttgtcgac gaaacttcca
    33421 gttctaattt aggattgcgc tcgatccaac gcaaaatcgt ttttatttgt aatgatacgg
    33481 ctgtttttga aagaaagaaa atgtctgcta tcttttgcat actgacatac cctttttgaa
    33541 acaataagta cgtcacaata gttgaagctc ggaaatccat gctggaatag attttatgtt
    33601 tcgtcacctc ttgtatgatt tgctgtttag tttcagattc aattgattct aaaaaataac
    33661 ctgaacgttt atttgactgg ataatgattt tcttctcgta gatagcaagc atatttttga
    33721 tttcagtttg gatcgttttc ttgttggtac ctaaagcttc agctaattct tcgcttgtta
    33781 tccaatgatt cttatttctt aatactacaa gaatttggag ttgtcgttta tttaagtcca
    33841 tttgcttaca cctcctcttt gttataaaca tacaggaaaa acctctcaaa tgacaatagt
    33901 cacttgcaga ggtttaaaaa tcatttgaag aaagatctat attcaaggtt tttaccatta
    33961 gactggataa tttgtttata atattcaaaa ctatcttttt tcaatctcct aaagcttccg
    34021 ctcccatcat tttgtctatc cacataaata aagccatatc gcttcctcat ttcccctgtt
    34081 cctgccgata cgatatcgat tggtccccac cataagtaac caatgatttg gcaaccatct
    34141 tttattgcct cagcaatttc ctgaagatgt atttttagat actcacaacg ttttggatcg
    34201 tggattcgat tcaattcatc tggtgcttca tctagtccaa tgccattttc aactatgaat
    34261 aatggtaatt cataacggtc tgtcaattca ttcatgacat aacgaaaccc tagaggatca
    34321 attggccaac tccaaggttc gggagatttt tccttcaaaa agggattatc gaaaccattt
    34381 gctccgccag tatctacgcg catttctgct tcttttttat agacagcgct acgataataa
    34441 ctaaatgcaa tataatctgc cgtgttagct tgcaagcact gttcgtctcc atctaacatt
    34501 actggtgcac agtcttcttc cttccatgtc ctgtaaatat aagctggata atgccctcgg
    34561 cacatgacat cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc aaatacattc
    34621 tctggatcac agtcatatgc ataagtcgct aatgcagaaa agctacacat aatacccatc
    34681 ttagcatttg gatcgatttt ctttaataat tgagtagcac gggcgttcgc tagaaacata
    34741 tgatgacagg cttgataaat cacttttttt gttagtgtag caatgggttc aagtttatct
    34801 ctagcaggga atgtaaccaa tccagcagct gcataaggca tacgaaaagc attattgatc
    34861 tcattaaagg tcatccaata tttcactttc ccttggtagc gttcaaagac ggtctgcgca
    34921 taaaattcaa aaaagtcgat taattttcga ttttgccacc ccccatactc tcctaccaat
    34981 gctaaggggg tttcataatg actaagagta attactggct cgatatcata tcgatttaat
    35041 gtattgatta gtctgtcata aaatacgaga ccagcctcat ttggcttctg ttcgtctcct
    35101 cttggaaata ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc taactccttc
    35161 agtaaacgaa tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg agaaagataa
    35221 tggagtgatt cgtctaaagc aatctcccaa atcttttttt cttcattcca ctgaatacta
    35281 ggatgctggt tcatgatccc gataagcgta tctgtcacat taggtaattt gccgtcttcc
    35341 aaccatgcac cttcacattg attagcagct actgctccgc cccataaaaa attttctgga
    35401 aatatttcct gatccataac tatctctcct cgctatcaac aatttcttac ttgaagtata
    35461 gctcgattca tcaaaacaag atattctaac tatttcggca gaagagaaga aaaaaattga
    35521 atgctactct tcatttttaa tatttatata taacaaatac ataagacgat ccccgaaaga
    35581 aaacactcgt taaccatcta agcaaacgat ttattttact tccaacacaa atgcttcata
    35641 aggtcgtaaa tcaactttgc cgtctggtaa ctgtttcaga ggataatttg acacaataac
    35701 ccttaaagct tcacgtggaa tagtgaacgt ctgcttttca ctggacatat tacaaataac
    35761 gagccattgc tggtcttcat atatgcgaga ataggcaaag acatgctcag gcgtcttttc
    35821 cactaattca tagtctcccc agacgatgat gtcatgtttt ctacgcaaat cgatcaattt
    35881 cttgtacata taaaaaacag aatcttgatc tgctaacgct tcttctgcat tgatttcttt
    35941 ataatttgga ttgactcgaa gccatggaat tccagtagta aacccagcat gttcacgggc
    36001 gttccattgc ataggcgtcc tagcattgtc tcttcctttc gccttgattg attccaagat
    36061 ttcctctttt gtaaatccag aagaaatcct ttcgttatac atattgattg tctcaatatc
    36121 ttttgcttcc gcgatatcat cgattgggca attcgtcatg cctaactctt ctccttgata
    36181 gatataaggg gtgcctttca tcatatggag caagatagca aacatcttgg cactttcttt
    36241 tcgatacaca tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt gattattcca
    36301 aaataaacta ttccatcctt catcacctaa agatgtttgc catttggata atactttttt
    36361 cagttcagag atactcaagg gtttcaaatc ccatttgtct ttgccttctt gctgatctaa
    36421 tcctatgtgt tcgaattgga agaccatgga taattcattt cttttaggat tggaataaag
    36481 tttagcgatt tcggtagttg ctccccatgt ttcgccaaca gtcatcagat ttttgtcacc
    36541 aaaagttgct tgattcattt cctgaagata ctcatgcagt ttaggtccat tacttgtgat
    36601 ttctttatcc ggttctttgc caatcatatc gatcacgtcc atacgaaagc ccccaatacc
    36661 tttatcgatc cagaaattca tcatttgata aatctcttgg cgaacctgtt tatttttcca
    36721 attcaaatca ggttgacgct tgctatacaa gtgcagaaaa tattgccccg ttttttcatc
    36781 caactgccaa gcagaaccac tgaatattga gcgcaatcta ttaggctctt ttccattgac
    36841 cggatcacgc caaatataat agtcccgata aggattttct ttatttttac gggcctcaat
    36901 gaaccacgga tgttcatcag aagtatgatt caccaccaga tccatgatga tacggatatt
    36961 tcttttcttt gcttcttcaa tcagttcttc catttcttcc atcgtaccga attctgccat
    37021 gattgcttga taattactga tatcataccc attgtcatca ttcggagact cgtatactgg
    37081 agaaagccaa attgcatcaa tacctaaata cgccagataa tccaacctgg aaataatccc
    37141 ccgcaaatct ccaataccat cttgatttga atcttgaaaa cttcttggat agatttgata
    37201 aacaactgaa tggtgccacc atttttgttc catgattttc ctcctataaa tggatgtttc
    37261 tattttttgc ttaatcattt attttagtct ctctcactaa aaattgtaac gctttcaaat
    37321 agctattgca ccttgttatc ggtaacagaa taaaacaaaa aaacagctga ggaaattccc
    37381 tcaactgttt tttgaaagga ttgatttatg cagggaagaa tgctgatttc acaacttctt
    37441 tataaacggt gttcactcag ctgtcccttg gtattagctc aaaaaacggc aaaatataag
    37501 aaactatttt ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct
    37561 ttatttcaca ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat
    37621 tggagatact tcttttcctc ttttagctgc taacatggta attggataga agataaaacc
    37681 taatgcaatc ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga
    37741 agggatcgca atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac
    37801 tcctaccata atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga
    37861 aaagaacaag cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc
    37921 tccaacggcg ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa
    37981 ggaacccgca agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt
    38041 gttgtctttc atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa
    38101 gaaagtcaca agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc
    38161 tacatgtttt aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc
    38221 agaaggcatt ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ccatcccgat
    38281 gaagatgcca cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat
    38341 cgtcaaccaa gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga
    38401 aacgatgatc ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga
    38461 aatcgcatat ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat
    38521 tgtgatcaaa ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg
    38581 aatccccatt cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc
    38641 cgttccaatt ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa
    38701 tgcagtcgca gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt
    38761 tacaaataag atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt
    38821 taccgtggtg ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca
    38881 tccccctaaa tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac
    38941 aacgtgtttt atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc
    39001 aaaatccgaa cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt
    39061 tggattacaa gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc
    39121 aatagtgtga tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat
    39181 cgacaggatt cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt
    39241 ttgtcttctt ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc
    39301 gccgatactt caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt
    39361 attcaatcag acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa
    39421 ttgaaagtca agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag
    39481 atagtttcaa attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt
    39541 gaaaacttct gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg
    39601 agtacaatac tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt
    39661 ttttctacaa agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt
    39721 ttttgtatca tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat
    39781 tccattatat cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc
    39841 aatagtttct aggattgctt tcctccacaa acttttttta caaaaaaaaa acggcaatga
    39901 atgccgctct tcttatttcg attcttttat aattctgctt ctgattcatc tttggttagt
    39961 gtgtgtctaa cactagttac gccaaatgtt tgatcgattt tttccttcaa cgcttcgatt
    40021 gcctgtgcgc cacgtgccgc ttgttcagca ttgatggatt caatgaccag caccgctgct
    40081 tgtgtttctt ccgtcagcat cgttctttgg gcttcacgcc agttgagaca gcgacagatc
    40141 gctccttgtt catcatagta gatgatttct tccggaagtg ccggagcatc ggaatctgca
    40201 cctaaaggca ggaatgattc gccaccttgg gcttttccta aatgcagatt tcctgcgatc
    40261 ttttcgatat cttctccgcc gcaaggtacg ccaaaagaca aggaaatact attatagata
    40321 tctacaagcg gattgattgg gaaaaattcc cgttcttgat ttgcacgttt cagtaaagct
    40381 tcaatagaag aacgtgcacc ttttttagtc ttgaatgtgg taaatgcctg acgccactga
    40441 gcaatgactt cgttttggct gaatgtttct tctgtaaggt aatttcttgc ttcttttgct
    40501 gcctcattca ataattgatg atacgtttct tcactttctt ttgttacgtg gttatcgata
    40561 ccatttacgg ataagatttc gatggttgct tcaggaaata cttcccagaa attggtatcc
    40621 actattactt ttttcatctg ttcacacctc ttgattagaa taattgcttg atgaatagat
    40681 agtctgtttg gggatcatct cccatataaa aaacatgttc gctgtatttg ataaatcctt
    40741 ttttacgata aaaagcgagc gcaacatcgt tgtgttccca aacaccgagc caaatggctg
    40801 ttttttgctt ttcttttgct ttttcaatag ctgtatctag taaaaatgta ccgatacctt
    40861 gatgtttaaa tttttttcgt acatagattc gctcgatttc taaaaaatga tcagccattt
    40921 cctcagattg actcgttcct gtatttagtt tcaggtaacc cgcaggctct tgatcgatac
    40981 tcactaggaa aaaatcggtt tctttatctt ttagctcttc tgtaagtttt tctagtgcat
    41041 aagcttgtgt caagtaatca gctaaatgct ccggtgaatt atcttttcca aatgtatctg
    41101 taaatgtttc gatactaatt tgttgtagta agtctacatc tgaaggcgtc atttttcgaa
    41161 tttcgtattt attcacggct tcttcctttc aatagatcct tttattgcct tttttgacaa
    41221 aattccaatc ttcgctaaca ttcttgcata ctttatttaa aagatattcc aattgttttg
    41281 cttcttcgtc agaaagacca tttaatgcga ctgcattgga gtagttgttt tctcgaatga
    41341 tgaaaggata gatttctgct ccttttttag tcggatagag tttcttgatt tttttatttt
    41401 ctttatcttc caaacgttcg atcatcccat tggattctaa tttcttgatt gctcgggcag
    41461 tcgttgtacg atctactttg atcatttctg ctagtttttc ttggatgatt ccaggatttt
    41521 cgcaaatacg tactaaataa agatactgtc ctcttgttaa atcgacttct ttgaattcga
    41581 tattgctaat agaatctagc gctcgggcaa tcattccgat gtctcgaagg atttcagcca
    41641 tttcatccca cctttatttt gtttgtaaga ctagttcacc tctttttgtt gcaaatgcaa
    41701 ctattttcaa taaaaaaatc aagttctttt ttaataacta gtaaagtata ttcttttctt
    41761 cttttacgaa aaagctcaat cacaatagat ctggtttttg ttttctaaaa taagtaactg
    41821 tttcttcgtt gttcgctttt ctgatagctt tggcaactct tctatactaa aaaatcccat
    41881 gttggaagtt tcattattct caataaactt ggcctcttca ccgtggatcg cacaagcaaa
    41941 aacaagttta taatactgaa aaagctgtgg aatatctttt tgtttattcg tatcaaaaac
    42001 tgctcgcaac tgagtagttt ctactgttaa tcctgtttct tcatagactt ctttacgcac
    42061 attttcttca ggagacaatc ctatttcagc aaatccacca ggaagtgacc attctttcgt
    42121 ccgtttatct tcgattagta ggaccttgcc atcttttttg atataggcac gaacatctac
    42181 ttttggtgtt ggatgtcctt ctccaatcgt tgtgagcgct tttatttctt cgaaagtttc
    42241 ttttcctatt tcactgatca gttccaatga aatcgtccgg atctcctcaa aacgctcttt
    42301 atcaaataca tcttttccat aatatagacc agcttgagaa agtgcgagta gtcttttgta
    42361 ttgagccaac ttcttgttca ttctcttccc ttctttttta gcaaatgtat gactatgtaa
    42421 tcaagtatag aagactggtt taattacggc aaataaatat aatgaaatct gcaataaata
    42481 aaagaatgtt aaaaaactcg agaacaaaag acattcgact tctctggggc tgtgaataaa
    42541 tggcgttcaa aagcttgcat ctgcggtaat aagccccacc aaacgaaaaa tatgacgagc
    42601 tattcttgtc tggctgttct tattaattga ggcaggacag ctttttcaca acctccccac
    42661 taaacggtgt tctcctacca actcctcggt cacaagtcac aatattcatc aaacatgaga
    42721 agctgttttt ttgaaattgc tccttgtgac tcggagctga acgacaggct cacgacctct
    42781 cggtaaacag cgttcaaaag ccaatttttc gatattaagc catttctttg ttacttattg
    42841 tcattgattt ctttttctaa tttatccaat agtggagtgt aatccacttg agtatagtct
    42901 gttacatcaa attcatatag tgttcctaac tggaatttgt catattttcg gtcggcgatg
    42961 atctttttga atgcttcttt cgtaatatga ttggtccctt tcgttcgatc ttccaatacg
    43021 tcaccatagt gataatgatc catgatatag ctcaaatgac gatctgattc acgatcacgt
    43081 ttgtaccgac gttgccataa cacttcaaag tctgctgtta ggcgaatcgt gatcacctga
    43141 tatccgtatc tatctgctaa ttcttggagt cgtgcttttt gcttatcact aaaaggatac
    43201 tcggacacaa tcactttctt tcctgcttcc atatacaaat caagtgcttt gtaatagaaa
    43261 ggataaactt tcgtcctttc ttgttccgcc cgttcttcca aattgttaaa cccgatggat
    43321 tctgcatagt attccttgat ttcatcgagc gtgatggcat acatatctgg aaatcgttcc
    43381 cgtagtttat tcataagata tgtttttcct gtaccaggac taccagccag aagaatgaaa
    43441 tactttttca tctcatttat ttcctaagtt aattggactt tccaagttca taaacagtcc
    43501 aatcttatgc ttgacaattt cttttactgc ttcatttgct ggaatcacat tatggcgaag
    43561 agacttgttg atttctgttt cactaaaggt ttctttagct actttcgtcc agttgactaa
    43621 aagctccgtt ccaacattga atttgcggat cccgttattg atcaattcag gataatcttc
    43681 ttctttcaca cctgttcctc catggataac tagggggaca tcaactactg catcgatttc
    43741 tttcagcaag ggaatgttta cttctgtttt tgatttgaat tgtccatgat tcgtaccgat
    43801 agcaatagct aatgcatcca cgcctgtagc ttctacaaat ttcactgcgt cttccggtct
    43861 tgtatatacc ttgtcatttt cagcgacatg gattccttct tctgtaccgc cgattgttcc
    43921 aagttctcct tctacggaaa caccgtgcgc atgggcatat tctactactg cttttgtctt
    43981 caggatattt tctttgaaag gcagatggga cccatcatac atgacagaag tatatccgct
    44041 gtcgattgct tctttgatat catcaaagtc tcgggcgtgg tctaagtgca agactgcatc
    44101 aaccatttct tcttctgcaa tagctgtcat tacattgact aacacatgca tgccgatata
    44161 tttagcggta tcgacacttg tttgaataat gatcggcgaa cccatttctt ttgctgcgcc
    44221 tagcatagca ggaagcattt ctaggttatg cgtattaaaa gctccaactg taaaattcaa
    44281 ctcttctgct gtttttgtta cttctttcaa tgttgtatac atcttttatt gccaccttat
    44341 ctttttattt gtttcctttg atcgttactt gttttgcaat ttgtcgcatc tcatccattt
    44401 tgcccatgta gtaatcgatc ccttgatcat caccagcacg agcagcatct gcacgcttct
    44461 ggttatagag gctcatcagt tttttatacc catctccgat cgtctgttta ttttccaaac
    44521 tcttttctaa atattggatg gcttgatcgt tcacatacaa ttcagaaaat ttaagtccaa
    44581 cttgttcata aagtttcgct aatttatgct tatcttgggt agtggaaatc tcttgtttca
    44641 attgtgtaat ctctgattga atcttttcgg tcgcttctgg cgatagggct ttttcttctt
    44701 ttgccagaac ttttttcggt tctttttctt ttttcttaaa aaatccaaac atcattttcc
    44761 ctactttcaa ttaaaatgct ttcaaaactg ggcttaagat ccaggcatat aagagtgctg
    44821 cggctactgt atctacatcg gttgctgtag cattgttgaa tcccatcgaa ttaaagatcg
    44881 ttactagcaa agcgggcaat aatgtaataa agaaaccatg agcaataccg ccaatgatcg
    44941 ctcctcggcg tccgccaact gcgttgccaa atatccctgc tgtccctccg gcaaagaaat
    45001 tggttagcat accaggtaaa atcatcgcta agccgaacat gggcaaagta aacatggcaa
    45061 taatcgtacc aatcgttgtc gtaatgaaac ctaaaatgac agcatttggg ctataaggaa
    45121 agaaaactgg acaatctaag gcagggatcg catcaggaac taatttcata gctatccctc
    45181 gaaatgccgg cacgatttca cctaacagta gacgtacacc agctagcaat acatacacac
    45241 ccactacgaa ttggatggct tgtaaaaagg caaacatgat atagttttga tctcctgata
    45301 attcagaagc gaatggttcg cctgcaaata aaacagtgat gatatatagc gggaccatca
    45361 cgaccatgac agacaaataa gtatcttgca gaaattcgaa atgtgtcggc aacttgatat
    45421 cttcgatcga ctttttattt tcgcctttct caccaaaaag cttagcaaca ccggcttcaa
    45481 ataagtaccc gatcgtacaa aaatgaccta aagcaatatc atttgatcca gtgattttac
    45541 gaatgatcgg ttgcgctact gccggcatag caatggcgaa acaagctccg ataaaaccac
    45601 cgaccaagat caatacgatc cctcgcagac cggcaaaata gccaaagact gtcgtcatcg
    45661 ttgccatcca taaaattgct tgaccagtta aaaaaatgta cttccatttt gtgaaacgag
    45721 ccaaaataat attgaataca aaaatggcta agaacgtcag tgcaatatct ctacctaaac
    45781 ccaaatcatt cattgcttga ccattgatag attcaataga agggatgatc ccttgcatat
    45841 gaaatccttc agtaaagatt ttcccgaaat aagttaaact tccaacgatg atgctcgaac
    45901 cggcactcaa cacttgaaat cctaggagtg tctttaacgt gccagagatg acttgtcctg
    45961 ctgatttctt ctgcagtagt agacccaaca tagcaatcaa tgcaatggtg atcgatgcct
    46021 gagtcaaaat attttcgatg ataaaattga taacgcccat gttttgatgt gcaccccaaa
    46081 agttagacta gaaatctaat taaaaggggt gctttttttg cggaaatata catttgtttt
    46141 taagaaaaaa gtagtttcag actatttaaa taacgaaggc ggctacaaat atcttgcaca
    46201 taaatatcaa ataaatcgta cactggttag acattgggta aggatttata actatcatgg
    46261 ttgggaaggc ttggttggag gtggcaaaag ctacactaca aaatttaaac ttgatgttat
    46321 agaatatatg gaaacaaatg gtctttctat ccaagaaact gctaaaaaat ttaatatcgg
    46381 ttcaaataga actctaagta aatggataga gcaatatgaa gaaggcggtg cttcttcact
    46441 tgagagccaa aaaaggggca gaaaaattag tatgaattcc aagctaaaca ttcctaaaaa
    46501 acttaaagat gagtctcttg aagaagaagt tattcgttta agagcagaga acgcatattt
    46561 aaaaaagtta gaaaccttga ttcaagaaca agatttgagc aagaagaaat caaggttaaa
    46621 acaatccttc agctcaaaaa agaatttaaa ttgaatctat tactttcaat tgcacaatta
    46681 gcgaagtcaa cttattatta ctgggtaaaa aaattagata agccagataa atatagcaag
    46741 ataaagcaag aaattacagc gattgtaaaa gaatcaagaa actcttatgg ttatcgtaga
    46801 gtcactttag cgttaaagat gaaaggatat acaatcaatc ataaaacagt tagaaaatta
    46861 atgtcccaaa tgggacttac ctgtcaaatc cgaataaagc gatataaatc ttataaagga
    46921 acagtaggaa aaattgccaa gaatgtgtta aaacgaaatt tttcagtaga tacacccaat
    46981 aaaaaatggg tgacagacgt cactgaattc aagataaaag gaagaaaaat ctatctatct
    47041 cctatacttg atttatttaa cggagaaata attagttata gtatatcaac tagtccaaca
    47101 tataaactga ttgaagaaat gcttcaacaa gcaattaaaa aaaagggaac tgagggttca
    47161 ctgattctac attcagatca aggatggcag tatcagatgc cacaatatca aaaaaaatta
    47221 aaagagaata acattattca aagcatgtct agaaaaggaa attgcttaga taattctgta
    47281 atagaaaatt tttttggtgt gcttaaatca gagttttttt atcgagaaaa attccgatca
    47341 attgagatat ttcaaagtaa attaaatgag tatattaggt ggtataataa caaaaggata
    47401 aaactaaagt taaatggact atctccagta gaatatcgga aacagtccat taaatagttc
    47461 aacttttggg gttcagttca taaggaggca gttttaataa atgaaaaaag atgtggggaa
    47521 tatagatgtc taaaaaagga tcagttttac aaaaatgtct tcagatcatg cccattattg
    47581 gtctgttttt tttctttgtg ttgattcttt atgcttatca tcatgggatt tttcgatcga
    47641 gcacgtcttt gcaagcgttt atccagcaat ttggcaagta tgcagtgatg atatttgttc
    47701 tgttgcaagt gatccaagtg atcattccta ttttgccggg agggatctcc tcagtagcgg
    47761 gcatgctgat gtttggcaat ggatttgggc ttttatatag ttgtattggc ctagtgattg
    47821 gggaagcaat cggatttttg cttgtccgtt actatggcgt agcctttgtc cagcttattt
    47881 tatcgccaaa aaagtatcag aaatttgatg aattgctgac aagaaaaacg aaagatatta
    47941 aaaaagtact agttgttacc ttgctgcttc cctttgcgcc agacgacttg atctgtctag
    48001 tagctggttt gacgaaatta tcttttaggg aatttatgca gattgtgatt ttcttgaaac
    48061 cgtggtcggt gggtgtgtat agcatgatca tgctcttttt attccatcaa gcgcaaggtc
    48121 atttataatg taaaaaagga cgtgggtgta tggaaaaaat aaaagtaaca gaaaatgaac
    48181 tggatgagct gatggcagtc atacaagaag tatggccgga agtttttgtg ccgatcattg
    48241 gtcaaaaaca agtggattac atgctgaaaa cttatcagtc aaaaaaacag attcaaaaag
    48301 aactgacaga aggtgtgtcc tactttttat taaaaagcga agaaaagact gtcggttaca
    48361 ctgcttatga agagataaat ggaaaaattt atttaagtaa attatatttg ctaaataaag
    48421 tacgtggtca aggattgact tcttcagttt ttcgctggta tgaagaactt gctgcgcaaa
    48481 caaaggaaag agagatcttt ttgcgagtga atcaggggaa tcatcaagca atcgaggttt
    48541 ataagcatga aggatttgag attacagaag aacttatttc agatatcggt caaggatttc
    48601 aaatggtcga ttataaaatg aaaaaagctc tcgcataaac ataggaactg tgttaaagta
    48661 aatttatgcg acgggtcgag aaggactctg gtttacagag ctcctaattg attttggagg
    48721 cacaggataa ttgatttcta tctttatcac ctgccggatg caggggtgtc gggcaagact
    48781 tttgtggaga agggcttgtc tgcttgagta atcaagtacc gtaaaaatgc agaaagaagc
    48841 ttatgcttct ttctgtattt ttttttacaa gaatatattc tgtaaaattg gtttatttcc
    48901 cctttaatgc taaaatcgaa gagcagatta gttataagac tgtttaagga gccattgtca
    48961 tgttcgaaaa actcaaacga tttgggcgta attttacaca aacaacgccc tttatcttaa
    49021 gtaacacggt tatttttatt ccttatcttt tatttttggg actaacagac tcgcataatt
    49081 ggctgacgat tttgccattt acgctgtttt atgcctttcg gatgactgga ctatttttag
    49141 tcaacagtct acgatttggt ctagatagtt acacgttgct gatgatcgct ctgctgattg
    49201 gtggtgctgg ttcattgctt ggaatattag gcgtatttta ttttccttta ttttattttt
    49261 caagtgtttt gctagggctg agtgcagcat ggcttgtacc tgctaatacc acggtcaatt
    49321 ttcacgaaaa gacgcaaggt tttatcaata tggatgggaa aaagtactta tttgcagcta
    49381 tctggctatt tctcctgtat caagcgattg agttgcctgc accgactcag attgcagcaa
    49441 gctttactat ttatacactt ttttacgtca tggcctacca tacggttagt cattatccgc
    49501 gttacgaact ggatttcaaa gacatcaaaa gaaatctggt agctacgcga gagcttgttt
    49561 tatttttgat attctttggc ttattgtttc ttttgagaaa tgcccgattg ttattcgatg
    49621 aaaggctttt tgatcttgct atttatggtt tcttgctgct gttcatcttg ttcgtgtttg
    49681 ttttaggaca ccagaagaag gaatggaagt tgccaagctg gctaaatctg tttacttttc
    49741 taaatggaat gctagggaat ttcctttttt tatttagcag tttttatgtt ggcatactgt
    49801 acggattaga aaaactttcg atttttatgt atttgcctta tgcagcaggc ttgattgcag
    49861 caaaaatagt cggaccatgg attttgaaac gtttaacatg gtctccattg gctatgcaat
    49921 tgcttggact gagttcttct ttattggtcc tcttgattcc aaatggtttt gcgataggtg
    49981 tctttctgct tagtttctgg cacgtagtaa caggcagctg gctaaataag gaatattatc
    50041 aaactgatac agctattcca atggatcgcc ggatcgttgt caagtatact actcaaaata
    50101 aagggagtgt gatccaccag tttttattga tgacgctttt gtttgtttta gcaaaagaaa
    50161 tgggagagcc aatccatcta ttattgatca cgctgaacca tgaatcagtg ccgatagaaa
    50221 gtgtacaact gcttatcttg gcaaaatgga tcaatgttgg tcttttactg agcggattag
    50281 taactgttta tcatttatgg aagaaggcga aaaagaatga ggctattcat tgatggagat
    50341 ggttctccag taaaagaaga tgtcataaaa ttaggagaat cctatggtct tgacgtggtt
    50401 atcgtgacca gtgtggacca ttatacgacc aaagaatacg ctaatcatgt ccaaatcgtc
    50461 tacgtagacc gcggagctga tagtgcggat tataaaatcg tcggtatggt taagaagaat
    50521 gactttttga tcacgcaaga ttatggttta gcttcacttg tattgccgaa gggtgtgcga
    50581 gtgttccatc actctggaaa agaatttgac gaagcgaata tcggcacgct tctagaacaa
    50641 cgttatcata gtggaaagtt aagaaaagct ggcataagga caaagggtcc aaaacccttt
    50701 acggagaaag atcatatgtc ttttcgtcaa gcgttaaaaa aggcaattga ctaaggttta
    50761 ccaagtacag aagatagtag gtaacgaagg tattttattt atgctcgaat acatcaaaaa
    50821 gggaacaggg aaacaggata aatgtgttcc atgttccctt tttgattgtt cttattactg
    50881 ggtgcagaac agctttttca cattttttat tctcggtgtt tcagaagtag cctcttcgaa
    50941 aaataagccg aattttcaca aaaatttgag aaacaattta tgtaaaaccc cttcttattt
    51001 cttgaagctg attctttctg tcacaacctt tttctacttt ttcctcttct tatacatttg
    51061 gatcaatgca tcgattgcca aaaaaataat gacgaatgcg gcaatagcat acgtaaatat
    51121 ccctaataag gaaacgacct ctgcgttttc tacaggtgct gcttggacaa gcagggagga
    51181 ccctacgatc aatgcagcta aaatcatacc gaaaacaagc cgattgatca tactttcgat
    51241 cctgctcaat aatttgtctt gctttttcaa ttccaaattg acttttcctt gtcccgaagt
    51301 ccacatttct aatagatggt ggagctgttg agggatctta ggcgcagcct tcatcccttc
    51361 caataagtct aatccgcttt gtttcaatgt atcttctaca tccagatgag tgagaaaata
    51421 atgttgagca aaaggcgaag cgacttccat gagggaaact tcaggatcga ggacgcggat
    51481 caccccttct aatgtcccaa atgctttcag caaaagcgtg atatcacgat cgaactgcag
    51541 attattttga tgacaaatcg tcacgacatg tgtgaatact tcttgcaagt tgatttcatc
    51601 aataggagaa tcataatact gttcaagaaa acttgtcagt tcttggtgga accgactttc
    51661 atcaaacgag ccttcttgct gacaaagtcg aaggaccgct ttttcgattc gataagcatc
    51721 tttcgtatac agcgctaaaa cggcttgtgt gagtttttgc ctaagtcctg cagaaagatg
    51781 ccccatcata ccaaaatcga tgtagttgat ggtatatggc gcaacagggt cttctgcaga
    51841 agtggaggct cgaaaagcaa aggaaccgaa ctctttttca tggactgttt cagtttttcg
    51901 gctagcttgt gtctgttctt cttttgttag tacatggaat agcagattcc ctggatgagg
    51961 atcagcatgg aaaaaaccat catcaaacac ctgtttcata aagttctcaa ctaaaagctt
    52021 tgcaacttct tgtttcagct gcttgttttg aattccagtg ataaaagttt cagttttgtt
    52081 ttcggcgttc attaaatgat tcaagttttt accagacatt tcttccatca cgatcacttt
    52141 tttcgaacaa aaagcgtcat aaatcttggg gctgcggatc tctttccagc cattgttctt
    52201 ttgatagaat tgttcgccat tttgactttc ttttaagaag tccatttcat tgatcaagga
    52261 acgttttact tcttggagta ccccttttag atcaactacg ctagtttcag gaatgtattt
    52321 gatcaaaggg atcgctcgtt caaatagttg aagatcaagt cggatctctt cggcgatgtt
    52381 agggtgttgg atcttaacga ctaccgcgtc accgttcttc agcttggcac gatgagcctg
    52441 ccccatagaa gcagaggcaa aaggcgattt tgaaaaatca tcgaatatat ctgataatga
    52501 aagagaaagt tctgtttcga tcgtcttttt gaccgttgaa aatgtgtcgc taggtacact
    52561 atcttgtaat gttttgaatg tttgagtgaa ggcagaagaa agcaaatctt ctcgaacaga
    52621 aagcatctgt cctatcttga tgaaggtggg gcctagttct tcaaaggctt tttttacctg
    52681 ttcgggattt ttttgctgta caaagtttgg cactacattg taattgatga acactcgaat
    52741 gatttctgcc aagcggctgc gtggatttgt ttgcgccatc atcatccctc cttttcttta
    52801 tcataacttt ttataaaaaa cgggacaaat aatctagctg acaaaaaata aatgacgctc
    52861 gttcttttag aagctggctc gtttttttat aaataagtta ttcgtttatt tttgttttgt
    52921 gctaaaatat gagagtaaat cactgaacga tttagaatac aggaggacaa tcttttgaag
    52981 attactttac tatatggcgg acgcagcgca gagcatgaag tgtccattct ttccgcattt
    53041 tcagttttaa atgccattta ttataattat taccaagttc aactcgtatt tattacaaaa
    53101 gaaggacaat gggtcaaagg tccattacta acagaaaaac ctgccagcaa agatgtcttg
    53161 catctttcat gggacccaag tggacagaca gaggaaggct ttacaggaaa agtgatcaat
    53221 ccgggcgaaa tcaaagaaga aggagccatc gtttttccag ttttacatgg gccaaacggg
    53281 gaagatggaa cgatccaagg cttcttagag acattgaata tgccttatgt cggcgcaggc
    53341 gtattgacca gtgcatgtgc catggataaa atcatgacca agtatatttt acaagctgct
    53401 ggtgtgccgc aagttcctta tgtaccagta cttaagaatc aatggaaaga aaatcctaaa
    53461 aaagtatttg atcaatgtga aggttctttg ctttatccga tgtttgtcaa accggcgaat
    53521 atgggttcta gtgtcggcat tacaaaagca gaaaaccgag aagagctgca aaatgcttta
    53581 gcaacagcct atcagtatga ttctcgagca atcgttgaac aaggaattga agcgcgcgaa
    53641 atcgaagttg ctgtattagg aaatgaagac gttcggacga ctttgcctgg tgaagtcgta
    53701 aaagacgtag cattctatga ttatgaagca aaatatatca ataataaaat cgaaatgcag
    53761 attccagccg aagtgccaga agaagtttat caaaaagcgc aagagtacgc gaagttagct
    53821 tacacgatgt taggtggaag cggattgagc cggtgcgatt tctttttgac aaataaaaat
    53881 gaattattcc tgaatgaatt aaactctatg ccaggattta cggagttcag tatgtaccca
    53941 ctcttatggg aaaatatggg cttgaaatac ggtgatttga ttgaagaact gatccagtta
    54001 ggaatgaatc gataccatca gcgtcaatct ttttttgaaa aaaatgaata aagagaaata
    54061 aagaagaggc tggagtgatt gcgtaaccgc gttcattcta gcttctaaat acgaatagag
    54121 aaatttactt ggaagtgaga gtcatgcgaa cccgccgttt aggccaaacg cttttttcac
    54181 aacctttttt aggagagatc tcaatgaatc taacaataca agaaattgca caagccgtgg
    54241 gcagcgaatg ctctgcactg acaaagatta ccaatgtcga atttgacagc cggaagataa
    54301 ccgaaggaag tttattcgtt cctcttgctg gtacgcgaga tggacatgaa tttatccagc
    54361 aagcgatcac aaacggagca tctgcttcat tttggagctt gcctgttgaa caagctccaa
    54421 aaaattttcc agttattcct gtagcagatc ctttgaaagc catgcagcaa ttggcaacct
    54481 attacttgga aaaagtaggc gcagaagtcg ttgcaattac aggaagcaat ggtaaaacca
    54541 ccacgaaaga tctaaccgcg tctgtcttgt ctgaaaaata caaaacatat aagacgcaag
    54601 gaaattacaa caataatatt ggtcttcctt acacgatttt gcacatgccc caagatacag
    54661 aaaagatcgt tttggaaatg ggcatggatc acgccaatga aatcacggaa ctttcactta
    54721 tggctcaacc aaaagctgca gcaatcacaa tgatcggtga agcacacata gaaaatcttg
    54781 ggtcgcgaga aggaatcgct aaagccaaaa tggaaatcac tgacggactt gtatcagacg
    54841 gattactcgt tgtgcccgca gatgaaccgt tgctgacacc tttaacggca acattagtac
    54901 aaacagtcac aacattcggt attggaaatg gcgatgttca tgcggaagtg acagcaaaag
    54961 gaaaagcaca aaccgatttt gtagtagaag gagaaacatt tacgatcccg ctgcctggaa
    55021 gttataatgt aacgaatgct ttgattgcgt atacgatcgg gcgcttcttt ggactgtcca
    55081 tacctgagat acgccaaggg ctcgcccatg tttctatcac tcaaaacaga accgaatggc
    55141 taacggcagg aaatggtgca gctattttga gcgatgtcta caatgcaaat ccaacagcca
    55201 tgggactggt attagatact tttaaaaatc tgccaacaaa aggacgcaga atcgctgtgt
    55261 tagcagatat gctggaatta gggaaagagt ctttaaatat gcatgcgcaa atgagtgaac
    55321 atatcgatcc tgagaaattc gccatgattt tcctttatgg ggaacaaatg catgcactaa
    55381 aagaagaatt gaaccaaaaa tacccccaac tacctgtgtt ctattttgaa aaagaaaaga
    55441 ataaattgat tgatgctatc aaacaaaatc ttgaagcaac agatagtatt atgctaaaag
    55501 gcagcaacag catgggctta tttgaagtgg tagaacaact tcagcaaatg aaataaaaat
    55561 ctgtaaactt gcagaaaacg aaaaactatg ttagaataac ctattgccga aagatgaaga
    55621 gaaattggac tggttttgcc atagaaacaa cttgttgaag ccagatgtca taataatgat
    55681 aaagctgaac gcgacccgtt tggcttttct aatgcctaaa cttcgcaaag tgatttttcg
    55741 gtggtgccac aggggctaag aatggataaa aacgtgcgtg gccactgtca aaaagaaaca
    55801 atacactttg accgaacaaa tcgacggggt agcaaaaaag ctacatcctc agttaacaac
    55861 catataggag gattcatttg aaatttaaag aactagaatt atcaccagaa ttgttgaaat
    55921 cagtcgaacg cgctggtttt gaagaagcaa cgcctatcca agcagaaacg attccactag
    55981 cattagctgg aaaagacgtt attggacaag cacagacagg tacagggaaa acagctgcat
    56041 ttggattacc aatgttagaa aaaatcgatc cagatcgtca cgaattacaa ggtttagtta
    56101 tcgcaccaac acgtgaatta gctatccaaa cacaagaaga attatatcgt ttagggcgcg
    56161 acaaaaaagt acgtgtacaa gccgtttacg gcggagcaga tattggccgt cagatccgtg
    56221 gcttgaaaga tcgtccacat atcgttgtgg gtacaccagg acgtatgctt gaccacatta
    56281 accgccacac attgaaactt ggaaccgttc aaacattagt tttggatgaa gcagatgaaa
    56341 tgttgaatat gggcttcttg gaagatatcg aaaaaatcat ttcacaagtt cctgatcaaa
    56401 gacaaacatt acttttctca gcaacgatgc cgccagctat caagaatatt ggcgttaaat
    56461 tcatgaaaaa tccacatcat gtgaagatca aagcgaaaga aatgacagct gacttgatcg
    56521 atcaatacta tgtccgcgca aaagaatacg aaaaattcga tatcatgact cgcttgtttg
    56581 atgttcaaac acctgaatta acaatcgtct tcggacgtac aaaacgccgt gtagacgaat
    56641 tggcacgcgg attagaagca cgcggttacc gtgcagaagg aatccatggc gatttgtctc
    56701 aacaaaaacg aatgagcgta ttgcgttcat ttaagagcgg tcatttagat atccttgtag
    56761 cgacagacgt tgctgcacgt ggattagata tttcaggtgt cactcatgtg tacaactatg
    56821 atatccctca agatccagaa agctacgttc accgtatcgg acgtacaggc cgtgccggaa
    56881 aaggcggtat gtctgtgact ttcgttacac caaatgaaat ggactacttg catgtgattg
    56941 aaaacttgac gaaaaaacgg atgacgactt tacgtccacc aaccgaaaaa gaagcattca
    57001 aaggtcaatt aggtgctgca gttgaacaaa tcgaaacaaa acttgcagaa aatggtctag
    57061 ataaatacct gcaaacagca gataaattat tagaagaata ttctgcacaa gatttagttg
    57121 cacttttgct aaaaacgaca gcaaaagatc cagctgatgc agtgccagtg aagatcacac
    57181 cagaacgccc attgccaatg cagaaaaaag gatacaataa aaatggcaaa cgtggtggcg
    57241 gaaacaaccg taatcgtcgt gacggcggta attatcgtgg aaacaaatca aaaggcggtt
    57301 atagcaaaaa taataacagc caaaaagatg gcggtaaacg tcacaatgat aaaaaacgcg
    57361 gatttgtaat ccgttcaaat aacgattaaa cataaaatga aagtttctct tttttaagag
    57421 aaactttcat ttttgttatt tgtggactta tttgtggcaa aatggttata atttggtaga
    57481 atgagagtgt tctaaattta ttgaaaagag aaaagcatat gataaaagga attgggatcg
    57541 atgcagtcga acttcctagg atcactcggc tgatcgaaga aaaaccaaaa ttcattgcac
    57601 gaatcttgac ctctgatgaa atgaaactat tccagtcctt gccttttcat cgccaagtag
    57661 aatttttagg cgggcgttac gcttgcaaag aggctttctc taaagcttgg ggaactggga
    57721 tcggaaaagt tacctttcaa gatgtcgaga ttttaaaaaa tgaaaatggt cagcctgttg
    57781 tgacgcgttc acctcatgaa ggcaatgtat gggtcagtat tactcataca aatgaaacag
    57841 cttttgcaca aattattttg gaagactaga aagaaggaaa aacgatggtt gtcgcttggc
    57901 accgcccaac gaaagcagtg atacataaaa aagctataac tgaaaatgta gcaaatgaag
    57961 ttgcaagact tccgcaagga aaagaattgt ttgcagtagt aaaagcgaac ggatacggtc
    58021 atggagctat tgaaacagct gaggcagcag ttgcaggtgg agcttcgggc ttttgtgttt
    58081 caaatttaga tgaaggagtc gagttgagag aagctggatt tactcagccc atccttatct
    58141 tgaatatggt tccttacgac gcactgactg tagctgttgc tcatgattta tcagtaactg
    58201 caggtacacg ggaatggttg caagcagcag ctgctgtttt agagaaaagt aagctagaga
    58261 cgcctctttc gattcatcta aaagcagaca ctgggatggg tcgtatcgga ttttgtacac
    58321 ctgaagaagt aaaagaggca gctgcattca tcaaagaaag tcgtgtatta gaatgggaag
    58381 gtttatttac ccatttttct acagcagatc aggcagatga tacatattgg aatctgcaaa
    58441 aagaacgctt tatagaagtg ctgaaaaaac ttcccgaact tcctcgatat gtacatgtca
    58501 gcaatagtgc aacggcttta tggcatgatg aaacaatcgg caatatgatt cgttatggag
    58561 tagcaatgta cggacttaat ccctctgggc acgcactacc ggaagtttat ccattgcagc
    58621 cagcacttga gctggtttct gaattgattc aagtcaaaaa actcccagca ggtgaaggaa
    58681 tcggctatgg agaaacatat atcactcctg aagcagagtg gatcggaacg ataccgatcg
    58741 gttatgcaga tggctggcca cgaaagatgc agggtttttc tctattggta gaagggaact
    58801 actgtgaaac gattgggcgt gtatgtatgg atcaactgat gatccgattg cctcaagaat
    58861 ttcctgtcgg aacaaaagtg acgcttatcg gcaaaaatgc agacaaagaa atcactatgc
    58921 aggatatagc agatcaatta ggtacgatcc actatgaagt ggcttgcgga ttaggacagc
    58981 ggataccaag agaatatcaa gaataaggag gatcctatgg taaaaagagg agatatttat
    59041 tttgctgatc tttctccagt cgtgggatcg gagcaaggcg gaacacgccc agttttggtt
    59101 attcaaaata atctaggtaa ccattttagc ccgacgatca ttgttgcggc cataacggca
    59161 aaaatggcta aacctaagct tccgactcac attgggatca aagctactgg aacaggaata
    59221 gaaagagact ccgttatcct gttggagcag atcagaacga tcgataaatc aagattgaaa
    59281 gaaaaggtct gccatttgga tcgacccatt atggcagaag ttgaccgagc attgaaaatt
    59341 agtgtgggta tcgaagcact gactcctgtt actaaataaa tatagcgaag taaccatgca
    59401 gcctttacat ataaaaaagg accagctgtg cgtaaaaaaa gatacgtctg cttctagtac
    59461 cagcatttct gctagaagaa gcgaacgtat cttttttgat gatccaagga gtggtccatg
    59521 cgccctcctc ctttacatta cagccagact tggattacca gctggctttt tttactcctg
    59581 gaatcaaacc tttatgtgct aattcacgga attttatacg ggacatcccg aatttacgca
    59641 tgtatccgcg agggcgtcca tcgattaaat cacgattttt caatcgttct ggacgagcat
    59701 ctaaaggaag tttacgcaag ccttcgtaat ctttttgcgc ttttaattcc tggcgtaaag
    59761 cagcatagcg ttcaatcgtt tcttgctgtt tttttgcttt ggcaatcttt gattttttag
    59821 ccatcaacaa attcctccaa ttatttttta ttagcgtaaa tcgtaatctt tacgctttta
    59881 gataaataat ttaacacgtt acgcgaaaaa agacaactat tttgttttga atcatgcgcc
    59941 aaaacgaaaa gacatttttt ttgtcaaaat aaaggcataa acgtaaactt aaagaaaaag
    60001 ggagatggtg accatgcgaa gatttgcaat taacggttac ggagaagcac aagatgtttt
    60061 tgaagaaatc gatgctcatc ccagagaagt cggttctgga catgttcgtg tagaaataaa
    60121 agcattcagt gtgaatcctt atgatgttgc cttgcgttta ggagaaatga aagagatacg
    60181 aacattgaaa ttcccttatg taccaggtaa tgatggcgct ggtattgtga cagagattgg
    60241 ttcagatgtc acaactgttc atgtaggtga tcgagtagct gtccatgcag tcggcgggac
    60301 ttacggagaa gaagtcgtgt tgccatcagc aaaagtagca aaaattccag ataaaatgag
    60361 ttgggaagaa gcagccggaa tggtcacgcc aggaattacg gcttataatc taatcaacca
    60421 tttgacggaa atccagccga cagatactgt aatgatacta ggagcatcag gtgcggtcgg
    60481 gtcaagtctg atccagcttc tccatgaaaa gggaatccga atcttaacca gtgcatccag
    60541 taaaaatgag gaaaaggtaa aaaaattagg agcttcagca tttgctgctt atgataaaac
    60601 aaatccaggt ctgcaatttg ctgatcaggc agaccttgta atcgatgcga cgaaaggaag
    60661 tatcaaagga gaaaccggca tccagatcat gaaaccaggc ggcaggtatg ttgcattaaa
    60721 tgacctccca gatctagatt tgcggcaaaa aaaggaaggc ttttatgaat cgtttgttcc
    60781 aagaaaggaa tatctagatg cagaagcctt tgctggcata atcaaagcct accaaaaagg
    60841 agcctttcat gtttttattt caatgaatct gtctgcttca cttaagcatg tcatccaagc
    60901 acatcagcta gttgaaggac atcctcctgc tgggaaaatc atcctgtcat tcgaaaaata
    60961 agaaatacag gaataaaaaa cgaaaaaagc tgaaacattt ttgtaggggc aaagacaaaa
    61021 aatgttttag cttttttgcg tatacgtata atagataaaa actttggcat aaaattatct
    61081 ttccctttta tttcatgata aagtaaaggc tgtaatcctt aattatttct caatatgtaa
    61141 gagattacaa tgagacaatt gccgtgatat gtccaaaaat tcgacttttt tgaaaaaagc
    61201 cgattgaaaa aagtatgaaa tacatggtaa agtaattcag ctgaaaaaag aagaaggggg
    61261 agaactatgc agtcaatcaa aaatacgctt cgattatatt tgcttgactg gaaacggatt
    61321 ttcaaaaacc cgattgcaac acttctgatc atcgcgatca tgatcatccc ttcgctatat
    61381 gcgtggttta acatcaaagc attatgggat ccatatggaa atactggaga gctgccaatt
    61441 gctgtttaca gtgcagacaa gccagcagag tttcaaggta aagaagtggc cattggggag
    61501 caagtcatcg aatcgcttca taaaaataaa caattaggat ggcaattcgt tgattcaaaa
    61561 gaacaattag aagacggagt ccgttccgga aagtattacg caggtattta cttgccaaag
    61621 gatttctcag aagatctgct gagttttact agtggtgata tcaagaaacc aaaaatcgaa
    61681 tatacggtaa atgaaaaaat caatgcgatt gcaccgaaaa tcaccgataa aggagcaagt
    61741 tcgatccaat cgcaaatcac aaatgaattt atcaaaacag ccagttcgac tttattgaaa
    61801 gtattcaatg agataggtta cgacatcgat acgaacctag taagtatcaa taaagttaaa
    61861 gatatgattt tatcaacaga tgaaaatttg gacacgatcg acggctacac aaaacaagtc
    61921 ctcgaattac aaagtcaact acctgaaatc aaggaaaaac tgaataaagc aaatgaattc
    61981 gtggattaca tccctaaagt agatgagatg ggtgaaaaag tcgttgcatt gaatgacaaa
    62041 atgcctgagt tgaaagatca agcaaaaatc atccttgatt tgcaagaaaa aatcccagag
    62101 atccaaaatg ccggtaagca actagcagag atcgacggcg attttgcttc gatccaagag
    62161 acaatgaacc aaggaatcga tgaagcaaaa caaggcttga cgatcatcca acaagtacaa
    62221 gatatcttgc cagatgtgaa aaatttagaa aatcaggcaa gtgatctagc aaaccaaaca
    62281 aaatcaggtg cctctcagct gaaagatgcg ataccaagca tcacaaacag tgtagatgtt
    62341 acattgaagt caattggaca agtggcaaca acgacaggtt cgttaataga tactgtaaga
    62401 acagctattg aagatggtaa gctgactgat gatgaaaaga aaaacatcag caatgtattg
    62461 aacggcttta ttgacggaat atctcaacaa caaaatgcaa tcgataaact ggtcgagttc
    62521 ctgactaaat tgcaaacaag tgctggaaat acgaaattac agccaattat tgatcagcta
    62581 aatcaattaa aacccttgct aagcaatctg caaaaccgcc tatctgcttt gaacacagct
    62641 gttcaaaatg aagacacgaa ggctattgat gaattgctag atcaaattga tgccgttgtt
    62701 aataatatca atggcatcat tgctggtatc aatacaggtc aaatcagttc cactgtcact
    62761 tctctactag accaagtcat cgatacgatt gggactgcac aaggcgcact gagcaaagcg
    62821 gaccagatcg actttgattc gcttctcagc tcaacgaaag caactgtaac gaatgcagtg
    62881 gcaattttag aaaaatatca aaaagaaatg cctgcaattg gacaagaaat ccatgatgcc
    62941 aatacaatgc tgaatggaaa tatggatacc atcgtcaatg gcatcaatga aggggcagac
    63001 ctatacaaca atgaattacc gatcattcag caaaaattag gattggccgc tgattttatc
    63061 caaaatgatt ggccagcact aaaaaatgaa atcaccggta cattgacaac agtaaatgaa
    63121 aaaatgccag aagtcgaaac agctgtgaac gctgctgcgg acttgatcaa caatgactgg
    63181 ccaaatatca aagctggtat ccaaaaagca gcagacgcga tccgaaaagg cgaacaagaa
    63241 gtcgatctag gcgaagtcat caaattattg aaactagacg caacaaaaga aagtgacttc
    63301 tttacacaac ctgttgagct acaaaccaat gcgctttatc caatcgcaaa taacggttca
    63361 gctagcaccc cgttttatac tgcactatgt ctgtgggtcg gcgctgttct tctttcaagt
    63421 gttgctacga cagaatacta tttaggaaag aaagaccgaa acaattttac gaaacgagaa
    63481 caattcgttg ctcgtatgct gacatttttg acaatggcga ttgcgcaaag cttgatcgtt
    63541 acattaggga atattttctt actaggagta gatgtccaaa atccggtcta cagcgtgtca
    63601 tttgctgtct tgatcgcctt agcgttcatg atgatcgtct atgtcttggc tgcattattc
    63661 gataatgtcg gtaaagggat cgctattatc atcttagtac tgtcgatctc tggcggaggc
    63721 gggaactatc cgatccaagt ctcagggaaa ttcttccaaa tgatcaatcc gttcttgcca
    63781 tttactcatg cggtaaactt gttacgggaa tctgcaggag gaatttattg gccaaatgct
    63841 acgaaagcaa tctggatcat gatcggtttg tttgttgtct ttggaatcgt aggaacggca
    63901 atctatccgt tcatcgaaga aaaaatgacg aaattcaaag aagcggcgca tgagagccat
    63961 atttttcatt aaaaaaagag gttgtgaaat cagtgttttg acctgagtat tagagaaaaa
    64021 aatggaaaaa tagttcctca tattttgccg tttttttgag ctaataccga gggtcagctg
    64081 attgaacacc gtgtaccaag aggttgtaaa aaagctgctc tgcatcaagt aataagaacg
    64141 accaaataaa aacagcttct tatgttttta atttggttgg gcttattacc gcagatgcaa
    64201 gcttttaaac accgtttatc aagaggttgt gacaactgcg ctatgcttcg agtagtaaga
    64261 atgatgggta gaaagcagcc ttacatctct cttccaaaag ttaagatatc aaaaaggata
    64321 gaacttgatt ttctaagtca agttctatcc ttttattata gaattcaaag aaaaagccca
    64381 taaaaaaatg ttagattttt gctgtattaa agcgtttgaa aaagcgcttg atatttttct
    64441 gtttttttcc aattattttc ttaaagttta ttgactttta aggtcagtga gcgtactata
    64501 acatcttgtt tactataaat tttcgactat aaaacttgaa gtaaaattgt gtgtaaagga
    64561 gtttagaaat tggattactt aaaaaggttg ttagtaggta agcctttaaa atctgctgaa
    64621 aatgatgagc acaaattaac ccgatttgct gctttggcat tattgtcgtc tgatgcgtta
    64681 tcatctattg catatggtac ggaacaaatc gttgtcgttt tagtcgcttt gtctgctgca
    64741 gccatctggt attcgctacc cattgccgct tttgttatta ttttattgat ttcattaacg
    64801 ctatcatatc gtcaaatcat ccatgcttat cctcatggcg ggggagcata tgtcgtcagc
    64861 agtgagaatc ttggaaagaa tgctggcttg atttccggtg gctctttatt gatcgactac
    64921 atgttgacag tagcagtatc tgtctcagca ggtgctgaag cgatcacatc agcggtgcca
    64981 gcactctacg gtcatcaagt cgctatttct gtgacaatcg ttttgctact gatgatgctg
    65041 aatcttcgtg ggctgaggga atctgcatcg ttcctattat ttccggtgta cacatttatc
    65101 cttgtgatct cgttactgat cgttgttggt ttatataata tcgtgacagg tgctgtgcca
    65161 ttgcaagcta ctgcacttcc aggagcggct gtgccgggtg tttctatcgc tttgatttta
    65221 cgagcatttt cttctggttc ttcctcttta actggggtgg aggcgattag taatgctgta
    65281 ccattcttta agaagccaag agcaaaaaat gccgctgcta cattgacgat gatggcattg
    65341 atcttaggtt tcttcttcgt ggggatcaca tttatcaact attggtatgg gatcgttcct
    65401 gaaaaagaag tcactgtttt gtctcaaatt ggtaaagctg tctttggaca tggtattttg
    65461 tattacgtcc ttcaatttgc tacagcgctg attttagcag ttgctgctaa caccggtttt
    65521 tctgcttttc cagttcttgc atacaatctg gcaaaagata aattcatgcc tcacatgtat
    65581 caagatcgtg gggatcgact aggctattcc aatgggatta tcacactagc gctaggttcg
    65641 atcgtattgc tgtttatttt ccatggctca acagaacgct tgattccgtt atactctatc
    65701 ggcgtgttca ttccatttgc tttatctcaa acagggatgg tcgtcaaatg gaaaaaagaa
    65761 ggaaaaagat ggttaagcaa atcgattgcc aatattacgg gtgcgttcat ctcatatgca
    65821 atcattgcga ttttgtttgt ctatcggtta ggggacattt ggcctttctt catcattatg
    65881 ccaatcgtta tgttcatttt ttataagatc catgatcatt ataaaaaagt agcagaacag
    65941 cttcgtttag aaaatgatgc aaaactacat gattatgacg ggaataccgt tcttgtatta
    66001 gtcgggaatg tgacacgggt aaatatcggt gcgttgaatt acgcacgttc gatcggtgat
    66061 tatgtcgttg cgatgcatgt ttcgctagac gaagatgtcg aaaaagaaaa agaaatcgaa
    66121 gcagaattta aaaagcattt cccagatgtc cgtttttcaa tcgtccactc ttcttatcgc
    66181 tcgattgaaa atccaattat ccgttatgta gatatcgtaa gtaaaaatgc agcgaaacaa
    66241 aactatacaa ctactgtgtt gattcctcaa tttgtaccaa acagacgctg gcagaatatt
    66301 ctccataacc aaacaagttt gcggttaaga ttgcgattgt cttggcgtga aaatatcgtt
    66361 gtcagcacct atagctacca tttgaagaaa taaaccaagt tttaaaatca aaaacagacc
    66421 ctctagctct taattgaagc tagagggtct gttttgtctt attctcagct aaaggtgccc
    66481 tctataagcc attaaagatg ggaacagcgt atgaaatgtt attattcttc ctatttagct
    66541 gtaaaacatt ttgattttga tgtcttgatc ataatttccg aatcctttgc caaataatgt
    66601 acagcctcct atattttcag cttcttcctt tacctcgact cttaagtgcc atgtgtcttc
    66661 ataagtagga atctcagcct cgtcaagtga ccattttttg ttaccataac tcagtgccat
    66721 ttttaattgg tgattcacaa acccaacggt ctaagcaagt atcaaatcga tagtgaacat
    66781 gaatgacagg agacgaaaga ggcaatcatt tttgaggcag tagaagactt gacggccaaa
    66841 atcagttttg aataaccgtt acaaatcttt cgtgcgtatc ctcaggttat tcaagatatc
    66901 aatcaagtca cactgcaacg aggaaccttc gtgtattggt gttgttgcat agactcacag
    66961 gctttggatt cgacctttgc taaagatgat acgttgttac caattacttt acgtttggct
    67021 accgctagtc gtaaaggagt aagaagtgag cgtggacaaa gatcctccct aaaaaggggt
    67081 ctttttctct atgtaaaaaa gaggaaaagt ttgctgaatt taagtgcttt attgtatttt
    67141 tttgacgtac ttaatcaagt acgctataat tatatcagaa aaaggaggag agacaatggc
    67201 taatacaacg atgaatccta gcactgctcg taaaaatttt tatcagttat tgaaagaggt
    67261 aaatgaaaat catactgaaa tcgagattat tagtgatcgt agtggaaaca atgctgtatt
    67321 gattgggctg gaagactgga gagcgattca agaaaccctt tttcttgagc aaacaggtac
    67381 tttagataaa gtacgagacc gcgaaaaaga cgacagtagc tttacgaata tcgatgatat
    67441 tgattgggaa gctctttgat gagtaactat acagtcgcaa tcaaaaactc tgcgaaagtg
    67501 gatttaagaa aaatcaagca aacgaattta aagaaacaat ttgaagaagt gattcagaca
    67561 ttgaaggaag atccatatat gcccacgcag tcttttgaaa agctgagacc tacacatgaa
    67621 ggcagatact cacgcaggct gaatagacag catcgagtgg tttacaaagt tgatgaggaa
    67681 aataaagttg tagaaattta ttctgcctgg acacattatg agtgaagtga gaagagccaa
    67741 cctttttaga tagttaacaa agagtctccc caaaaaggag actctttttt tgttctaaaa
    67801 ccaaccttct tggatttatt gcatgatttc tttaatttgt tcctatatct ccacctttga
    67861 agggtcattg tttgagttta ggaatggaat gttgacgggt ttatgtaaaa caaggaggaa
    67921 gaagtgtgac aaggaaaatc agtaaaattg gagcatcgtt gttagtgttg gcagtggcaa
    67981 cgaccatcga ttatcgccaa agtgaggcga agacaattgg aaatgtcgtt tatcgagaaa
    68041 cagccaaaag tatgttgaaa aaagcaatgg acaatcaacc ggagtcgtct tattggttcc
    68101 cagaggattc gttggcatgg tcttttggaa aagatacaga tacgaaatac aataccagtg
    68161 tcgttccttt agcagagcgg gtatccaaag cgacattgcc gaacatgaat gtcactcaaa
    68221 cggaagaagt taaagtcgtg gcgctttcgg ccatgaatag cagtatcaac ggcaatgcat
    68281 cacgagggat cgatatcttt tacgccaatg tcttttctta ttggcaatat atcgatcaat
    68341 tggtttacgg gggtgctctt ctggtgaagg atcatcgtat cgccaagtct agttgcgacg
    68401 gatgcagtcc ataagaatcg tgtaccggtt ttaggaaccg caaacctccc atggcgagaa
    68461 gctggaatgg ctagatatgt ttttagccaa ggatgaggat ggcaagttcc cgattgtata
    68521 aaagatgatc gaagttaccg atacggcagt caccagtttt gatgatgcag ctgaaggcat
    68581 cgaacaatcg aggactgatg gaaaaggctt aaatgaaagc cacgccaaag gatgtaagaa
    68641 ttgatgccca attcaaagaa caagcagacg agtatttaga gatcatgtgg tatgaattcc
    68701 atgacgagtg atgggaaaat ggattggcaa aatgccttag cggataagaa aaaaacttag
    68761 tcggtggatg cagatatgaa cccgttggca gtatgaagtt ggagtatacg gaatggataa
    68821 atagctaaga aaagcaactc tttgcccata aggagggaag aatcagagtg gattattgcc
    68881 ttaaaaatga cagaactaaa aaagcctttg cgaccaacaa atggtcgcag aagcttttgt
    68941 tttttacaat aacatcagcg tgatgtacgg ctggctggat ctagccgtct ttccaaaata
    69001 cctaaaagcc agtccgtgat gatcgccatt aatgcagttg gcaatgcacc tgctaaaata
    69061 atcgatgtgc cctctgttgc attggttcct cggataatga tatctccaag accaccagca
    69121 ccgacgaatg caccgatcgc ggttatcccg atggcaacaa ctaacgcgtt tcttatgcct
    69181 gccatgataa cagaaacaga gagtggtaac tctaccatga atagtcgttg tctagcagtc
    69241 atccccatgc ctttgccgac atcaaggatg tttttgtcta cttggatcat cccggtatac
    69301 gtattcttga tgatcggtaa gagggaatac aaaaatacag taacaatcac gacattgact
    69361 cccaggccta atccaatgat taaaatggag atcatggcta gagaagggat cgtctggatg
    69421 atattcgcca aacgaatcac ccagtttgct aatttacgtc tcctagaaat catgatccca
    69481 accggaatcc cgacaactgc tgcaaagagg acaccataga tagaaatcag gaagtgacgg
    69541 ataaattgtg caaagatata ggagccattt tcctggaaat aatagaaaaa ttgttgaaag
    69601 acattcattt tatccaattg cttcacttcc cttcaaaata gtggttttct tgtaagaaac
    69661 gttgtgctac tactgatggt tccactagat tattatctgc ttcgtagttc aattcctgca
    69721 ttttttccgt actgatggta tgacctaact tatttaaggc ttcttttaaa tgaggcgtat
    69781 cagctaatat cttgttgtct acaactggag cagcatcata tggtgggaag aattgacgat
    69841 catcttccaa catgactaga tcataactgg cgatccgtcc atccgtagag tagccaagaa
    69901 caatatccat ttttcctgct tcgactgcgt cataaacaag tccaatctgc ataggtaata
    69961 tcgaattgaa ggagaagcca taagtttctt ggaagccgtc ataaccgtct cctttacgat
    70021 tgatccaaga agtatctaca ccagctacta attcatccgc tacatttttc agatcgctga
    70081 ctttagatag atggtatttt tgtgcggtgt cttttcgaac aagaaagaca tacgtatttt
    70141 caaaactata ggacgggaac cacgtttgac cgtatctttt ctcaaattca tctttcacgg
    70201 tagcaaacgc ttttttcgga tctttttcag gaggaaggtt caatgtagtc gttaaatctg
    70261 ttcctgtgta gcgagccgct gaaattgaag catctccgtt catcatcgct tgatggttga
    70321 tagttgtagt agccagattg ttgatgattg cggtattttt atctgtataa tgttcaacca
    70381 ttccggcaac tagacttgct aagatctgtg cttctgaagt gattccacct gtgatcgaaa
    70441 tggtgtcttc atctgtattg ctggcaagac cagggaagga acaactcgca agtataagaa
    70501 gagagaaaag aaccatggtc atttttagta tacgtttcat tcttcattct cctctctaag
    70561 tgcgatcggt gtcagccggt gttccaagag acctagaagc caatcagcca atagtgctaa
    70621 aattgttaca gggatcgtac ctgccaaaat gagatctggt tggtaattat tcagtccact
    70681 aaaaatcaaa tcgcctaaac caccagcgcc gatataagag gcaagtgtag cccatgcaat
    70741 cacgtaaacg gcagctaaac gaataccagc catgatcgtt ggcatagcaa tgggcaattc
    70801 aacggaaaaa atggattgga actcagtcat ccccatgcct tttgccacat cccgataatt
    70861 ccaatcgaca tttttcatcc caatgtacgt attacgtaaa atcggcaata atgaataaat
    70921 aaagagtgcg ataatcgcag gtaatttacc tacgccaaat agcgggatca tcaatgccaa
    70981 taaagcaaga gaagggattg tctgcaaggc actggttaag ccaatcacaa tcgtggcagc
    71041 ttttggaaaa cgtgtcagta agacaccaag cggtacagca atgatgatac caatgatcaa
    71101 agcaagtcct gaaatataaa gatgttcaaa accttttgag agaatttctg aaccgtgctg
    71161 atgaaaaaac tgctgcatag gtcagacctc cttagattct tcaggagtag ctgactctgc
    71221 aacatcagta gttgcgggat cttctcccca aatcacatcg tagacgatat ccactaatga
    71281 tgcacgagtc aggataccta cgactttgtt ctgttcatca acgaccggca cgtatttcag
    71341 cccgcgtttt aaaatgcgtt gcaaggtatc acgaagatag gcggattctt ttacgtaaaa
    71401 aacatgttta ttcaaaatgt cgccgacact ggatactttt cctcgttttt tattcaacgt
    71461 ttctacatcg ataaatcctt ttaatacatt cgaattatcg gtaactaaca acgtatctac
    71521 acgtttttcc ctcatcagtt tgattgcttc ttgtaatgat ttttccggtg taatcgtgat
    71581 tgctgtattt agcataactt cgccgactct tgtcgcattt ggcttggctt gtagtaggcg
    71641 atcttctcca atcaactctt ctacgaaatc gtttgcagga tgacgtaaaa tattttctgg
    71701 tgtatcaaat tgatcacacg gccttcactc atgatggcga tacggcttgc tagtttaatc
    71761 gcttcatcca tgtcatgggt tacaaatacg atcgtttttc ccaaacgctc ttgtaaatct
    71821 ttgacaaggt cttgcaagga atcacgagtg attggatcaa gcgcaccgaa tggctcgtcc
    71881 atcaagatga tatcttgatt cgctgcaagg gcacgaacga ctccaatacg ctgctgctga
    71941 ccaccagata attcatgtgg gtagcgatct aacatctcac ggggcaattc cacaagatca
    72001 atcatttttt cagctgtttt attccgttct tcttgactga ctttcaacaa tttttggaca
    72061 agagtgatat tttcacggat cgtcatgtga ggcatcaatc caatactttg gataacatag
    72121 ccgatctgac gtcgcaattc cactggatca atttcttgga tggattttcc atcaatcaaa
    72181 attttccctt gtgttggatc agtcattcgg ttgatcatcc gcatcgtcgt cgtttttccg
    72241 cttccgctcg taccgataaa acaaatgaac tcaccctttt caaatgaaag attgacgtcc
    72301 tctacagcga cttttccacc tttataaatt tttgacacat tttgaaattc aatcatggtg
    72361 ccacctcttt ttctattttc aatgaagaac tatctattac tagtatgcct aaaacaatga
    72421 aaaaatacaa atagggtagt ttttttattt aggaaaacaa aaaatagaag tgaaaaagtg
    72481 ctgattttgc caacagaacg aaaaacatga tagaatgatg aagcacatga tagaaagtga
    72541 gtgacgatat gttaacaaca gaagattttg attttgactt gccggaagaa ttaatcgcac
    72601 aaacaccatt aaaagaccgc gatcattctc gtttacttgt tttggatcgg aaaactggag
    72661 agatggaaga taaacatttc catgaaatca ttgaagagtt acagcctggt gatgcgctag
    72721 ttatgaataa tacacgagtt ctgcctgctc gtttatatgg agaaaaacca gatactggtg
    72781 cgcatttaga agtccttctt ctaacgaata cacaaggaga tacgtgggag acattgatca
    72841 aacctgcgaa acgtgccaaa gttgggacaa gaatctcttt tggtgatggc agattgcaag
    72901 cagtagtgaa agaagaattg gaacacggcg gacggatcat tgactttgag tatgaaggaa
    72961 tttttctaga gattcttgaa tcattgggag aaatgccatt gcctccatat atcaaagaac
    73021 gtctggaaga cccagatcgc tatcaaactg tctatgcgaa agaaaatggt tctgctgcag
    73081 cgccaacagc tggattgcat tttacgaaag aattacttga tgagatcgaa gcaaagggtg
    73141 ttgaattagt ctatctgaca ctacatgtag ggttaggtac attccgacct gtcagcgtag
    73201 ataaaattga agaacaccat atgcatagtg aattctaccg tttgacggaa gaagcggctg
    73261 agcgattgaa ccaagtacgt aaaaatggcg ggaaaatcgt ggctgtcgga acaacttcaa
    73321 ttcgaacatt agaaacgatt ggaacgaaat ttaatggaga aatcaaagca gacagcggat
    73381 ggactgatat tttcattacg cctggctatg aattcaaagt ggtagaagct ttctcaacta
    73441 atttccattt gccaaaatcg actttagtca tgttagttag tgcctttgca ggaagagaat
    73501 tgactttatc tgcttaccag catgcgattg aagagcgcta tcgtttcttt agttttggag
    73561 atgcgatgtt cgtgaaataa ggttatgaaa agccgctagt tttgaataac aaaaaagctt
    73621 tacaaagatt ttatgacaaa gaaaaagccg agctctcgat aagctcggct ttcttgtacc
    73681 tcggaaggat tcaaataaac agaaatgatt gtaactttcc atcatacatg caaagcacgg
    73741 tgttgaattt cttcatgtta aacaaaaatt ttgttttcgt tacatcttca acatttgaaa
    73801 catctcttgt tcctattcaa ttttcagacg tttacgttat tcaattgtta cgtatcgcat
    73861 tacttcttgt ttcggatact tcgtgatcca aacatccata cgcattcact atttataggt
    73921 ttgtactcta cacgcatgtc tgggtgaaac ctgtacttct aacaactttg tttgcttaca
    73981 ctgttctatc gagaaattct tctgtctatt tgatggtatc ctccttcctt acatctatat
    74041 aatagcatcc tttggtagcg tttgcaaata taagcacttt aaattttacg ctttcttaac
    74101 aaataatcga taagcccttt atttgaagta aataaaaaaa ggatgttata gtaaaataag
    74161 tacctttttt tgtacgaaga ttttaaagga ggaataggat ggaagaatac aagcagacaa
    74221 tagaagaagt aaagaaggta acagaagcag atccagatac tgggttgtct tcacaagaag
    74281 tggatgaacg gcgaaaaaag cagggattga ataaattcga tgaggcgccg aaagaatcga
    74341 tgatcaaaaa attctttcga agcttgtccg attttacaac gatcatcctt cttgtagcag
    74401 cggtgatttc cttttatacc gcatttgcta cagaacatgg ggatttattt gaaggactgc
    74461 tgatcatcgc tatcgttgtg atcaactcgg tattggcgat tgtccaagaa ggaaacgcag
    74521 aaaaagcact ggaatccttg caagatatga ataaacaaac ggcaactgtc atacgagacg
    74581 gcaaagtgac cactgttgag tcagagcaac tggtagtggg tgatattctt gttttagagt
    74641 ccggtgatgc tatttctgcc gatgcccgtt tgatcgaagc atcacaatta cgtgtagaag
    74701 aatcggcgtt gactggagaa agtgaagcag ttgaaaaaga tgcagcattt attgctgaag
    74761 aagatgagtc attaggtgac caacttaata tggtatttaa aggatgtacg gttgctgcag
    74821 gtcgtgggaa agcgatcgtc actgccattg gcatggctac agaaatgggt aagatcgcgg
    74881 atcttttgaa cgaaaatact atgcaaaaaa cacctttaca ggtccgattg aatcagctag
    74941 gcaaacgaat cagtatgatc gctttagcgg ctgcggcttt agtatttgtg attggtgaac
    75001 tgcaaggaga accactcctt gagatgttca tgacttcgat ttccttggct gttgcagctg
    75061 taccggaaac cttgactgtt attgttacgt tgacacttgc atacggtgtg cagaagatgg
    75121 cgaggaaaca tgcgatcatc cgacagttgc cagcagttga aacgctagga accgctaatg
    75181 ttatctgctc agacaaaaca ggaacgctga cacaaaatga aatgcgcgtc cgccgggtat
    75241 ggagcaaaga agatgaagta acgaatatcg aagattcaat gacgaacagc gcgatggaaa
    75301 tattgaaaat ggctgcacta tgtacagatg tgacggtcga gcaagaagat gacgaccttg
    75361 ttatcaaagg taatccaaca gaagtagcca tcgtaagggc tgttgaagag aattatcata
    75421 caaaggcaga attagaagaa aaatatccac gagtaaacga gcttcctttt gattctgaac
    75481 gaaaaatgat gacaacagtt caccaaatgg gcgaaaaata catctcaatc accaaaggag
    75541 cattcgatgt tcttgctccg cgctttagtt caggcgatgt agaacaagcg gcgatcgtta
    75601 atgatcgctt tggtaaacgg gcactgagag tgattgctgt tggttatgct acttatgacg
    75661 aagaaccgca agatatttct tctgaagcac tggaaaaaga cttacgtttg attggtctga
    75721 tcggaatgat cgatccacca cgtccagaaa gtaaaggagc aatcaagcga gcgaaaaagg
    75781 ctggcatcaa aacagtcatg atcactggag atcatgtggt gactgccagt gccatagcga
    75841 aagaattagg tatcttgaat gatccgtcag aagcactttc aggatcagaa ctgcatcaat
    75901 tatcagacga agaattagat gcgcgtgtca aagctctctc tgtctatgct cgggtaacgc
    75961 cagaagacaa gatccggatc gtcaaatctt ggcaacgtac gggtgcagtc gtagcgatga
    76021 ctggggatgg tgtgaatgat gcaccagcgt tgaaagcaag taacgttggg tgtgccatgg
    76081 ggatcacagg aacagatgtg gcgcaaagtg ccgcagatat gattttgacg gatgacaact
    76141 ttgcgacgat cgtagacgct gtcagtcaag gacgttcggt ttatcagaat attcgtaaag
    76201 ctatcaactt tttgctaagc tgtaatattt cggagatctt tattgttttg attgcgatgc
    76261 tgttaggatg gggagcacca tttacagctg tgcaactact gtttgtcaat gtagttgctg
    76321 atggacttcc aggatttgct ttaggaagag agccagcgga acacggcatc atggatcagc
    76381 cacccattcc gaaaaatgaa gggatctttg ctcgaggttt attgcaaaaa attgccataa
    76441 atgcaggggt cttcaccatc gtaaccttgt ttggttatta tttaggtagt tatgtcgata
    76501 ctatcagccc atgggtagac gcaagtcagc atgtcggtca aactgttgca ttcttgatct
    76561 tagcttattc atcgattctg catgtgttca atgtacgcag cagccaatcg atcttcaaag
    76621 tcaatttagc gaccaataaa gctttggtag aaatggcgtt actggcatta gctattacaa
    76681 cggctgttgc tttgctgcca tttacgcaag aactattcgg cctagtacac atcagtttga
    76741 atcactggtt cttggtaggg attttatcta tcgtaccaat cgcggtgaat gaactgatca
    76801 agttccatcg attaccggaa gcagaagaag aggaatgatt aactgtttct aagttgattt
    76861 tctttttact ggaactatac aaaaaggagg tgtgacatga atgttgtcac acctcctttt
    76921 tgctaatggg ctgaatgttt cttttactgc ctctcgataa acggtgttct cctgccaccc
    76981 cctcggtcac aagtcacact ctcttttaaa tatcttttgg caattgttca taaaatgctg
    77041 cttttgttgc agaaatgtat gggctgagcc atagataacc aataccagca gttgctaatg
    77101 ctaagatatg ccagccgata aatgaaagat ctaaccagaa aagttgtcct ttataaccat
    77161 ccatcaattt tcgacttgca gtgatcgtat ccagtacttt aggtgtttgt cctgtttctt
    77221 ggacgatatc gtagtaaata aaatagcttt gagaataaga atagctttta atgattccag
    77281 gaataactaa tagcaaaccc cataatgttg taaagatcga gatcagcaca gctaagctga
    77341 tcactccgcc gataaacaaa cctttgaatc cacgaaatgc atctttgaat ggttcgatct
    77401 gatctttttg tccacgtaaa atatcaagat aagtccaaga aataccgctc atgaacaaag
    77461 cgctaatgat gctggaaata attccgccac cgcctcctga actaccgcct cctgaagttg
    77521 tcgcttctga gacgctcgct gatccatcat atatggtggt ggcaaacaag ataataggaa
    77581 tcataataag aagaatagta gcaataccaa tcaatgtagg gattagattt agtaaaacag
    77641 cttgtcccca acgtcctttt agactctctt ttgcttggcg ttttaattct gcagaagatt
    77701 gcatgatttt tccctccttt tctttaataa caccaatata ccatgtatca aaaaatattt
    77761 ctgtcataat gtttttcttt tttttgttat tcataaaaaa aggataaaaa aaagaatatt
    77821 aaataaagta gaaggaagcc gagggaactt gcaaaaagca cttgaaacga ttatgatggt
    77881 gggagttgtc aagatatttt atatagaaag tagggaaatg aatgacgcaa cctgccattc
    77941 gctatcgttt aatcaagaaa gaaaaacata ctggcgcgcg tttaggggaa ttgatcacgc
    78001 cacacggaac tttccccaca ccaatgttca tgccagtagg aacattagct acagtaaaaa
    78061 caatgtcccc agaagagttg aaagaaatgg gtgctggcgt tattttaagt aatacatatc
    78121 atttatggct acgccctggt gaagacttag tagaagaagc tggcggactg cataaattta
    78181 tgaactggga tcaaccgatt ttaacagatt caggcggctt tcaagttttt tcattaagtg
    78241 atatgcggaa tatcgaagaa gaaggcgtgc atttcaaaaa tcacttgaac ggttcgcgta
    78301 tgtttttatc accagaaaaa gcaatcaaca tccaaaataa attgggctct gatatcatga
    78361 tgagttttga cgaatgtcca ccattcgatg aaagttacga ctacgtgaaa aaatcagtag
    78421 aacgtacgtc tcgatgggca gaacgaggat tgaaagctca tgctaatcca gaccgtcaag
    78481 gattatttgg aatcatccaa ggagcagggt ttgaagatct tcgtcgtcaa agcgcgaaag
    78541 atctagtcgg gatggatttc cctggttact caatcggcgg gttatctgtc ggggaaccta
    78601 aatcagaaat gaatcgtgta ttagaattca ctacaccgtt gatccccgaa aacaaaccac
    78661 gttacttgat gggtgtcggt gctgctgatt cattgatcga tggtgttatc cgtggagtag
    78721 acatgtttga ctgtgtcttg cctactcgaa tcgctcgaaa tggcacatgt atgacttctc
    78781 aaggccgtct agtagtgaaa aatgcgaaat atgcccatga tttccgtcca attgatgaga
    78841 aatgtgattg ttatacatgt aaaaactata cacgtgccta tatccgccat ttgatcaaat
    78901 gtgatgaaac atttgggatt cgtttgactt cttaccacaa tttatacttc ttgttgaatc
    78961 taatgaaaca agtacgtcaa gcaatcatgg atgataattt gcttgaattt agagaagcct
    79021 tttttgaaga atacgggttc aataaagaaa acgcaaaaag cttctaaagg aaaatctttt
    79081 aaaaattaag taaaaaacta gtgttatcag catttttctg ctaaagtgtt ttttgtagat
    79141 acagaattag tgaggtgaaa aaaatgggaa cattaccaat gcttgtgatg tttatcgcgc
    79201 tattagcaat gtggtttttt atgtcacgtt cgcaaaagaa acaacagcaa gaacgtcaaa
    79261 acttgttaga tagcatgaaa ccaggtgatt cagtcgtaac gatcggcgga ttacatggag
    79321 ttgtttcaga aatcgacaat gacaaacgga cagttgtttt agactgcgaa gggatcttcc
    79381 ttgagtacga tcgtgcagca atcaaaacgg tcaaaccagg gactgtcgta acaaatgatg
    79441 caacgactac tgttgaaaca acagaagaac cagctgagac agttgaagta aaaactgaaa
    79501 caacagaaac gctggaagag acagaagaga aggaagaaaa ataagtatca aagcttattt
    79561 aaagacgagg tagggcagaa agatgtccta cctcattttt tattcaaaaa agaaagagat
    79621 aagagcatgg gatatattgc tcaatattga
//
LOCUS       DAZKMB010000002        68135 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000002 DAZKMB010000000
VERSION     DAZKMB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.002"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..2316)
                     /locus_tag="ACG8V3_000072"
     CDS             complement(130..2316)
                     /locus_tag="ACG8V3_000072"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0298647.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2534..3217
                     /locus_tag="ACG8V3_000073"
     CDS             2534..3217
                     /locus_tag="ACG8V3_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0298648.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3298..4017)
                     /locus_tag="ACG8V3_000074"
     CDS             complement(3298..4017)
                     /locus_tag="ACG8V3_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0298649.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4329..4535)
                     /locus_tag="ACG8V3_000075"
     CDS             complement(4329..4535)
                     /locus_tag="ACG8V3_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298650.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4780..5382
                     /locus_tag="ACG8V3_000076"
     CDS             4780..5382
                     /locus_tag="ACG8V3_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0298651.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5523..6803
                     /locus_tag="ACG8V3_000077"
     CDS             5523..6803
                     /locus_tag="ACG8V3_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0298652.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6816..7544
                     /locus_tag="ACG8V3_000078"
     CDS             6816..7544
                     /locus_tag="ACG8V3_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0298653.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7613..9136)
                     /locus_tag="ACG8V3_000079"
     CDS             complement(7613..9136)
                     /locus_tag="ACG8V3_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0298654.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9388..10569
                     /locus_tag="ACG8V3_000080"
     CDS             9388..10569
                     /locus_tag="ACG8V3_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0298655.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10629..12257)
                     /gene="treC"
                     /locus_tag="ACG8V3_000081"
     CDS             complement(10629..12257)
                     /gene="treC"
                     /locus_tag="ACG8V3_000081"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0298656.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12708..15419)
                     /locus_tag="ACG8V3_000082"
     CDS             complement(12708..15419)
                     /locus_tag="ACG8V3_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0298657.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15429..16130)
                     /locus_tag="ACG8V3_000083"
     CDS             complement(15429..16130)
                     /locus_tag="ACG8V3_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0298658.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16316..16915
                     /locus_tag="ACG8V3_000084"
     CDS             16316..16915
                     /locus_tag="ACG8V3_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0298659.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            16996..18009
                     /locus_tag="ACG8V3_000085"
     CDS             16996..18009
                     /locus_tag="ACG8V3_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0298660.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18117..19034
                     /gene="rbsK"
                     /locus_tag="ACG8V3_000086"
     CDS             18117..19034
                     /gene="rbsK"
                     /locus_tag="ACG8V3_000086"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0298661.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19062..19457
                     /gene="rbsD"
                     /locus_tag="ACG8V3_000087"
     CDS             19062..19457
                     /gene="rbsD"
                     /locus_tag="ACG8V3_000087"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0298662.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19457..20830
                     /locus_tag="ACG8V3_000088"
     CDS             19457..20830
                     /locus_tag="ACG8V3_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0298663.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21187..23331
                     /locus_tag="ACG8V3_000089"
     CDS             21187..23331
                     /locus_tag="ACG8V3_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0298664.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23383..24423)
                     /locus_tag="ACG8V3_000090"
     CDS             complement(23383..24423)
                     /locus_tag="ACG8V3_000090"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0298665.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24700..26502)
                     /locus_tag="ACG8V3_000091"
     CDS             complement(24700..26502)
                     /locus_tag="ACG8V3_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0298666.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26516..27268)
                     /locus_tag="ACG8V3_000092"
     CDS             complement(26516..27268)
                     /locus_tag="ACG8V3_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0298667.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27277..27639)
                     /locus_tag="ACG8V3_000093"
     CDS             complement(27277..27639)
                     /locus_tag="ACG8V3_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0298668.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27815..28687)
                     /locus_tag="ACG8V3_000094"
     CDS             complement(27815..28687)
                     /locus_tag="ACG8V3_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0298669.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28738..29226)
                     /locus_tag="ACG8V3_000095"
     CDS             complement(28738..29226)
                     /locus_tag="ACG8V3_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0298670.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29364..30155)
                     /locus_tag="ACG8V3_000096"
     CDS             complement(29364..30155)
                     /locus_tag="ACG8V3_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0298671.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30210..30818)
                     /locus_tag="ACG8V3_000097"
     CDS             complement(30210..30818)
                     /locus_tag="ACG8V3_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0298672.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30811..31128)
                     /locus_tag="ACG8V3_000098"
     CDS             complement(30811..31128)
                     /locus_tag="ACG8V3_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0298673.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31284..32012)
                     /locus_tag="ACG8V3_000099"
     CDS             complement(31284..32012)
                     /locus_tag="ACG8V3_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0298674.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32171..33052
                     /locus_tag="ACG8V3_000100"
     CDS             32171..33052
                     /locus_tag="ACG8V3_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0298675.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33289..34332)
                     /locus_tag="ACG8V3_000101"
     CDS             complement(33289..34332)
                     /locus_tag="ACG8V3_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0298676.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34335..35003)
                     /locus_tag="ACG8V3_000102"
     CDS             complement(34335..35003)
                     /locus_tag="ACG8V3_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0298677.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35145..37481
                     /locus_tag="ACG8V3_000103"
     CDS             35145..37481
                     /locus_tag="ACG8V3_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0298678.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37596..37907)
                     /locus_tag="ACG8V3_000104"
     CDS             complement(37596..37907)
                     /locus_tag="ACG8V3_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298679.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38088..39563
                     /locus_tag="ACG8V3_000105"
     CDS             38088..39563
                     /locus_tag="ACG8V3_000105"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0298680.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39610..40194
                     /locus_tag="ACG8V3_000106"
     CDS             39610..40194
                     /locus_tag="ACG8V3_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0298681.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40196..40615
                     /locus_tag="ACG8V3_000107"
     CDS             40196..40615
                     /locus_tag="ACG8V3_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0298682.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40896..41180)
                     /locus_tag="ACG8V3_000108"
     CDS             complement(40896..41180)
                     /locus_tag="ACG8V3_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0298683.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41532..42242)
                     /locus_tag="ACG8V3_000109"
     CDS             complement(41532..42242)
                     /locus_tag="ACG8V3_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0298684.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42239..43711)
                     /locus_tag="ACG8V3_000110"
     CDS             complement(42239..43711)
                     /locus_tag="ACG8V3_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0298685.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43849..44322
                     /locus_tag="ACG8V3_000111"
     CDS             43849..44322
                     /locus_tag="ACG8V3_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0298686.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44475..45026)
                     /locus_tag="ACG8V3_000112"
     CDS             complement(44475..45026)
                     /locus_tag="ACG8V3_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0298687.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45131..45661
                     /locus_tag="ACG8V3_000113"
     CDS             45131..45661
                     /locus_tag="ACG8V3_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0298688.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46144..46587)
                     /locus_tag="ACG8V3_000114"
     CDS             complement(46144..46587)
                     /locus_tag="ACG8V3_000114"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0298689.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46603..47763)
                     /locus_tag="ACG8V3_000115"
     CDS             complement(46603..47763)
                     /locus_tag="ACG8V3_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0298690.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48270..49970
                     /locus_tag="ACG8V3_000116"
     CDS             48270..49970
                     /locus_tag="ACG8V3_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298691.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50072..50905)
                     /locus_tag="ACG8V3_000117"
     CDS             complement(50072..50905)
                     /locus_tag="ACG8V3_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0298692.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51209..53374)
                     /locus_tag="ACG8V3_000118"
     CDS             complement(51209..53374)
                     /locus_tag="ACG8V3_000118"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0298693.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53634..55928
                     /locus_tag="ACG8V3_000119"
     CDS             53634..55928
                     /locus_tag="ACG8V3_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0298694.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55921..56586
                     /gene="pgmB"
                     /locus_tag="ACG8V3_000120"
     CDS             55921..56586
                     /gene="pgmB"
                     /locus_tag="ACG8V3_000120"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0298695.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56579..57586
                     /locus_tag="ACG8V3_000121"
     CDS             56579..57586
                     /locus_tag="ACG8V3_000121"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0298696.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57608..58627
                     /locus_tag="ACG8V3_000122"
     CDS             57608..58627
                     /locus_tag="ACG8V3_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0298697.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58908..60275)
                     /gene="nhaC"
                     /locus_tag="ACG8V3_000123"
     CDS             complement(58908..60275)
                     /gene="nhaC"
                     /locus_tag="ACG8V3_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0298698.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60384..61805)
                     /gene="tyrP"
                     /locus_tag="ACG8V3_000124"
     CDS             complement(60384..61805)
                     /gene="tyrP"
                     /locus_tag="ACG8V3_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0298699.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(62048..63925)
                     /gene="tdc"
                     /locus_tag="ACG8V3_000125"
     CDS             complement(62048..63925)
                     /gene="tdc"
                     /locus_tag="ACG8V3_000125"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0298700.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64216..65472)
                     /gene="tyrS"
                     /locus_tag="ACG8V3_000126"
     CDS             complement(64216..65472)
                     /gene="tyrS"
                     /locus_tag="ACG8V3_000126"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0298701.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65584..65789)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            66052..67353
                     /locus_tag="ACG8V3_000127"
     CDS             66052..67353
                     /locus_tag="ACG8V3_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0298702.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67422..68021)
                     /locus_tag="ACG8V3_000128"
     CDS             complement(67422..68021)
                     /locus_tag="ACG8V3_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0298703.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        20436 a        13811 c        12127 g        21761 t
ORIGIN      
        1 gaataaactg tgttccagaa atagcttctt cgaaaataag ctgaaatttc acaaaaattt
       61 gagaagcaat tttcgtacat tccttcttct ttcttgaaac tgaccacttc tgtcacaccc
      121 tctcttctat cactttcctt ttttcacacg actcccggtt tctttgataa atctcgatac
      181 tttcaatttt cttgaatttt tcatttgagg tacacataga tacagacgat ttcttgctct
      241 agtcatcgca acataaaata gtcttcgttc ttcttctatc gtcgttactg gttctgcttt
      301 tatttcatta aagtcgatta ctttcttttc tgtcgctccg ttataaggca aacttccttc
      361 gttacaacca atcaaaaaga catttcggta actaagacct ttacttttgt gaatgctctg
      421 gatacatagt gcatcaagtt ttccttgtct gtaagcaatc aagcgggttt ttaattcttt
      481 agctgtcgct tgggtttctt gcgataaggc ttggagtctt ctgaaagtag catgctgctt
      541 tacttcttct cgtatccatt cttgatcact ctggtcgatt tccttagcta tacgtggcag
      601 atgaagatag ctttgaacaa gcgaatagat ttcttgatgc tggtctgttt tacgcaacgc
      661 ttcctcaaac tctcggattt ctgcagattt cgattttaaa ttggggctta tcgacaaaat
      721 ataatctgca acggctatat tttgatattt aggagaaaag tattgatcgc attcactttt
      781 tttcaggtag cagtctgcca tcagctgccg gagcaattgc tggtaggcct gatttttctt
      841 ttgcagattc agtgctgttt gccaatgaac aattttttga tagaaatgtt ggaaagactt
      901 gataccaaag ataccttcat atggactatc tgcttccagt acaaaaggaa tattcgtttt
      961 cagcagagat tggacaagct gctgcgtttg tgaagctgat cggtataata tgattgattc
     1021 cttatatttc ccattttttt gaataaatgt ctgaatttgt ttgattagcc actgactttc
     1081 tgctttagta tcagaaaaca aataggcttc cataacacct gcttcttttt gtgctggaat
     1141 catcgccttt ggtatccgat catttttatt atagctgatc aatctatttg ctccttggac
     1201 aatagctgcc cgagaacgat aattagttaa taagaaaaga gttttggtag gatattcttt
     1261 ttcaaagttc ttgatgtatg ctggctctga tcctctgaat ccatagatac tctgatcatc
     1321 atctccaaca acaatcaact tgatcctgtt ttcctttgtc atccgtttca cgatttccca
     1381 ttgaagtgga ttagtatctt gaaattcatc aatgaataga taatcatatt tttggccgat
     1441 ataatgctgg atctcctcat tctccaaagc gcttttcaga ttttggagta catcattgaa
     1501 ctctagcaat tgctggcttc ttttttgttc ttggagttcc gtgagcaatc catacagtcg
     1561 ttctgatatc ggctgtcgtg cattcttatc aatggaatca tatttttctt taactaactg
     1621 aaccatttct tctactgaaa ggttcatatt ctgccaatag tctaaccgag aagcaatcgt
     1681 tttttctcca tattgatcaa atatataaag attatattta cgtatcagct gttgcaaaac
     1741 agcattcact ttttgtgcag tatcctttga aaagtctcct tgtattcctt gcattgctac
     1801 ttcaggaaat tttcgacaaa tttcattgta tatcctaaaa aagatactat ggaatgttcc
     1861 gatatggaca gcggtttttc tctggctgaa attatctact cgttttttta gttcatttgc
     1921 agcgttcctt gtaaaagtga tcattagtat gcgttcttcg tctacttgtt tttgcgtcat
     1981 cagataaaca gcttttgcac agattgtttg cgttttgccg ctcccagctc ctgctatgat
     2041 tcgaagatgt ttcttcatat catatgtgat agctgctttt tgttcttgat tgaagcgatt
     2101 ataggaacgt ttctccatca ttttaaagaa agagctgttt ccttcacttt cttttacttg
     2161 tacttcttcc tcaataagtc cgggtgcttc gccataagcc gcttcatagg tagcaatgat
     2221 ttcttcttgt agccgaaaga cttgttgaag ctttttatac ttgttctccc agcgtttcaa
     2281 ttctttttgt aatcggagaa tttctttttc attcaactga ttgatttcct tcccatcttt
     2341 cttctttgaa tttgtttatt tcaagttggt tatttaaagt ttcgttgtct attttttctt
     2401 tatactaaca gacctttcta aaaatgaaaa tgggaagcga tatgatttta gttttctgaa
     2461 aatttttcct ttttttcaat cgtttatgct aagataagga agacaaaaat attcataaaa
     2521 ggtgatgaaa aaaatgcaga cttcctttca cgaggggcgt acaataggac ttttgctgac
     2581 atttatcgga ggagccatgg acagttatac ttatatccaa tatggtgctt ttgcctctgc
     2641 tcaaactggc aatatcattc tatcgatcat ccaagcattt gacggagaat ggctaagtgt
     2701 tggtaaaaaa gtattgtcta cccttttctt tttcttaggg attcttctaa caaagtatct
     2761 aattgattat tttagaaaaa aagaaaaaca tttctggcgc ttgtttgttc tatattatga
     2821 agcagtgatt tttttctttg ttagtttgac acccgtccat gagcatcccg cattagtaac
     2881 gattttgatt gcttttactg catcgatcca gtgggtagct tttgataaaa tcaacgggcg
     2941 tgcctacact aatttattta caactggaaa tttaaaaggg gtagcaacga acttatatga
     3001 ttattgtatt tctaaagaaa aagcagattt tgatcgattt ttccactatc taagtgtcgt
     3061 tcttgccttt atcgcaggag ctattgtcct tgttttctgt catcatctgt ttggcgccca
     3121 ttcgattttg atcgtttcca ttttattttt gatcatggcc ctcactcaga catttcattt
     3181 atggcaattt tatcatacga atcggttcac tatttgatct caatgaatca aaaaaactaa
     3241 gcaagatgct gctcacagag aaatatctgc gagtgagcaa tttgcttagt ttttatatta
     3301 tttagttgta aagattccct ttgctttcgg gttatttgta gtgtagcgga tcgactgggc
     3361 atctgcctct tctgtcattt tctttgcccg catcttgatt tcttgtacga tttgttctcc
     3421 acatgccgga tcggctacga aatcatattt gtccccatcg atcactattt tgggacatat
     3481 atcaaactgt tcataccact cttcgtatcg tcgattgagt tctttatagt aggaatagag
     3541 ttcaggatct tgttctagct gttcatattc ccggcctctt tttttgattc tcgctaacat
     3601 agtttcaaag gatacacgaa tataaaccag tagatctggt ttcttttgtg gcgaaatttc
     3661 ttctaactcc ttcagcatcg tttctaaaag accttcatac tgctttactt ctacttcaga
     3721 aacacgccct aaatctgcat tcaaatgaaa cagcatacta tcttcgtaga tggagcggtc
     3781 tagtacgttg tcatcatggc tgaaagcatc tttgattgcc aaaaagcgtt tgttcaaaaa
     3841 gaaaatctgt aataaaaaag tatacttttc tggattagaa taaaatagcg gcaatacttc
     3901 attatcttcc acgttctcat aaaaaggttt tgtttctaaa tcttgggcaa gcatttcagt
     3961 tagtgtgctt ttccctgcac cgatcgttcc tgcaacaatg atcacaatta tcggcatcct
     4021 tcctgtaata aaatctgttc ttcattttac tacttcattt atagaaataa aagccttgac
     4081 tttatctgct ccttaaaagc tacttctcta aaagtagcca aaagtaactc ctattcatga
     4141 aaataaaggc tgtgacaaaa gtcttgtcgc agcccttttt tcaaataaac agtattctcc
     4201 tgccaacacc tcggtcacaa ggcaatattc ataaaacatg aaaagctgtt ttttaacatt
     4261 attccttgta actcagagct aaacgggaga ctcacaacct ctttatgctc tcaacatttt
     4321 atctgttttt attcttctat tgtacttgat ttgcgtacaa ctaactgcca gtgcagatta
     4381 tgagcggtat cattgtcttc tactgcgcat ttcatgacac gtatcatcac actattttca
     4441 tagatttttt ctatttttcc ttttattggt gattcaaata aatcagattt aaatgcatat
     4501 gtttcgccga cttcaaacaa ttgttcattt tccataagaa tccctccatc accttttata
     4561 aggtcagtaa ttttttaaca taaatcactc gaaaaagcac aaacttgtca taaaaagtac
     4621 ttttcgtatc aaaactgtca ctttttttgg taaggttctt ggaaaacgaa gattttttta
     4681 ggagatcatg acggttttga tgtcaaaacc actttttatg aaacaaaaga cgttttttat
     4741 tttttttagc ataaaatttg tggtggaggt acgtatgaga tgaaagataa agttgtgaac
     4801 aaattattag aagccttatt cagcggagac aaatcggaag aagatatggt gtatgtccaa
     4861 gtaaaatttg tcttagaagt tttattgaat aatcttggaa aactagcggt cgttctagtc
     4921 ttttctctgg tgacaggctc atgggcggaa acaggaatca cctttctttc ttatatctgc
     4981 atccgcagat atgcttatgg attgcattcc gactctgaat tcgtttgtct gctgtggaca
     5041 ctactttatt tatggggcgt tcccttggtg atgaaacatc tgcaattgac tatctccttc
     5101 ccaatgatgg tctttttgtt aatcagctgc tttttgttac tgcttcgcta tggatcaaga
     5161 ggtacagcaa tcaatcccat tgaacccgag aaacgtcccc cattattaaa gaaggcgatc
     5221 agcatgtttc taattttttc tctcattacg ttatttttct cagcttctta cttttctacc
     5281 tatttgctac ttgggattgt tttagaaata gctacgttgc ttcctattac aaactacttg
     5341 atgaattttg gaggaatctt tcatgaaaaa aacaatcatt aaacaattaa ctaacttatc
     5401 tgactttttt gtatctggct gctgctttgg tatgagttct gaattcaaaa tccctgaaaa
     5461 attaaaaaaa taatacgcca attgtgctat aatttggtaa aaatcaatgg gagctgtact
     5521 ttatgttttt atggtccata gcgttgcaat atttgattta cacaatttgt atcttattga
     5581 ttgaccatca catccgatac aaatatctgt ttgcgggagt cattctgatt tcttgtattt
     5641 tgattggatg ggtctattcg attttaggga tttttactgc tctaattgct ataggaatgc
     5701 tgatgcttac ttttttcttc ttttcgactt cgcgtcaaca attggtttac gcgcttcctt
     5761 ttgggttatt gacgtcgctt ttaggagatc acttaacaag catcggcgat ttttttgttt
     5821 tgaaatcttc ggttattgaa ccaggagatt cattgcagta ttttcacatt gtcttatccg
     5881 cttttttatc tgctttatta gctttttgtt taaaaaaatc gttagagact tggtttcaca
     5941 ctgtggaccg ccgtatgatc ggtacgatcg ggacattgat attatttaca tactatatca
     6001 ttatttttta tacccgtttt tctggtgaaa cacctgatgt cttagctttg aatacgagtt
     6061 ttttcattct atatctgtgt gttggcctga tcatcttggt ttattattgg aaaatctcta
     6121 ataagaaatt agctgatcag ttattagagc aacaagtcag gatgcaacag gattatatca
     6181 aagatctaga aaaaaactat caagacttga gagaattcaa acatgattac caaaatctgc
     6241 ttttttctct tcacagctat atttcagaag gcgacttaga aggattacgg tcttattatg
     6301 atcaaacgat attacctaca cgccagatga tgaacctttt tccggccaat ctaagtatgt
     6361 tggataatat gaaagttcct gagatccgta gtttactgtc attaaaactc atgatggcac
     6421 aggaaaaagg gttgatcgtc cagcttcatt ttcctgagcc aatcgaactg gatacgaaac
     6481 atacggtaaa ccttgtacgt atgcttggta ttatcttgga caatgcgatt gaaggagcta
     6541 gtgctgcaaa gaagaaaaaa atcgaattga ccatactcaa gaagaatcat tccgtgatga
     6601 ttcgtgtgat caatacaaca acaaatacat taccgttaaa ccagttgaaa caaaaagggt
     6661 tctcaacaaa aacaaatcca aaaaacgaag gccttggctt gtttatcttg gatgaattga
     6721 caaagaccaa tccttatctt ttccttgaaa catcaattga aaaccagcgt ttttctcaaa
     6781 cgatttatat acaaataaaa taaaggagta atgatatgat tcccatttat atttgtgaag
     6841 acgatccaaa acaattagaa gttttatcaa cggtgatcaa aaatagaatc atgattgaaa
     6901 acttagacag ttatgtccat atttctacct ctgatccgga agaacttctc aatgcagcca
     6961 agatcagaac ttcttctttt ggtatttact ttttagatat cgaactagaa gacagtgaaa
     7021 tggatggtgt agcgatcggt aaattgatcc gtgaattaga tcctttagga aaaataatct
     7081 atgtgacttc ccatacggaa atgtcgatga aaatcttaaa aagcaacatc gaaccgactg
     7141 attatatcgt caaagaagat ctttatgatt taaaagaaaa tgtcgagcat atcctgagca
     7201 aaattttcga agattttcaa atgtcccaaa tccaaaaaga tatcttcaaa atagagttta
     7261 atgatgagat caaattttta ccgatcaaag acatccaata tttttctact tcgctaggag
     7321 cgccacataa actggaagtc catttgactc atgcccaatt acaattctat gagaagatca
     7381 aagaaatcga gaaaatgcat cggaatttta tccgttgtca taaatcctat gttatcaata
     7441 cacaaaatgt gcggtcgatt aataaaaaaa ccagagaagt cacgttggca aacggcgaga
     7501 ccatcccagc ctctatccgt ggattaaaga aattgatttc ataaaaagaa ccgaatagtt
     7561 tggaccgact aaatgaccag ccgatccaaa cccttcggtt cttttttgcc tatcagtgca
     7621 aaagcatttc ctgtgcaatt tcacgtccat ccattgtatc tggataaaat gttggccaat
     7681 tctccatttc tttcagcaat acttcttgac tttcatttcc tgcatataaa tgaaaatgtt
     7741 cggagtcagc cggtgcaatc gtgtgatcac tgaattgaat atatctaaaa gcgccacttt
     7801 ccttatcttc tgcttcgaat agatacctta cgccacggtt tcctgcctcg taggtcaaga
     7861 tttctttccc tacataacga taatttgaac gtttcgttgt tccattctta aacgtaaatg
     7921 ccatctcttt ctcactgatc ttgattgaag caatatctgt ttgatagccc ttcgtataat
     7981 attctttata ttcttgtgct gtcttatcct gattcaattt ttctttatat tcaaaaacag
     8041 gatcaaaagt cccatcttct acatacgtat ctactgtttg ccaagttcct gcccaatctg
     8101 aaagagagcg atcttgtaca tcctcgtctt caaaataacc gttgtagacg ctcttttctt
     8161 ttttgggggt caaagtatct tctagcgggt tttctgtctc tgtcgttttt ttcaacgctt
     8221 cgaggtttgc ttccatgata gaaagatagt tttctccatt ttctatttgt tcattcgtca
     8281 atccttccaa aggattcaat acttctaaag atacacctgc ttcgtttgct aacgttctgg
     8341 caatactatc tttggcgttt tcttcaaaat aaatatagtt gattccatgt tctttaacaa
     8401 agtgtttcag ttcagcaatt ctcgccgcag acggttcttc actagaagaa agaccagcaa
     8461 tcgatacttg attcaatcca tattctaacg ctagatagtt gaatgcagca tgttgcgtga
     8521 caaaagatct ttgttttgct tgcttaaagg tttcttgata aagctgatgg agtgcttgaa
     8581 gtttctcggt ataggctgct gcattttttg tccatacttc ctgtttttct gggtaagctt
     8641 caatcagctg atcacgtatg tttgctacct gcttgattgc taaacttgga gctagccata
     8701 catgtgggtc tagctcatgt gtatggcctt cttccccgtg ttcatggtca tgatcatgtc
     8761 cttcttcact tcccggaagt aaaagcatac cttctgtcgc ttttatcgta tggacattgc
     8821 cttcttgtaa tgttttttgg attgcgggga cccatgtttc catgttttca tcattataaa
     8881 caaagatatc tgtatcttgg atctttgcca tgtctttagc agaaggttca taatcgtggg
     8941 aatcagtccc tgctggaatc aataactcta cttctccttc atccccgacg attgcttttg
     9001 taaaatcata catgggataa aaggtcgtca tcacgctcaa tttttcttta ctgtcctcgg
     9061 ctttcccact tgccccgcaa gcagttaaca tacctgctaa aaccagcatg gccaatatac
     9121 ctatcaatcg tttcatttat gacactcctc ttctcatctt ctttcatttg aatgttccga
     9181 ttcgcttttc gcaaaccgta atagttacgt tttagcaaca ggatttaatt tatcatatgg
     9241 ttaaaagaac tgtcaagcgt tttcttattt taattgatga gaaagtttat atgaatgatt
     9301 gacgatattt ttgcaatcaa cctctatcat tttcctttta tacccgcttt tgtctataat
     9361 gaatttatag ataaaggagg aaaaagagtg aaaaaagaaa cagatacaac gaatcgtttg
     9421 atccgctttt taggaggaaa aacctcttac tatgttttag gaatagttat cttacttgcg
     9481 ctgatcattt attttttcca tcagatctcg tttatattca agcctgtcgg aatcattatc
     9541 gctactatct tgcccccatt attgtttgca ctgatcttgt attatctttt ggaaccagtc
     9601 atcaaacgtt tgagcaagca cctttcgagg aacttgtcag tcattatcgt ttatgtgatt
     9661 attttatttt tattggggct tggaagcata tggcttattc cttttcttga agaacaggca
     9721 aagacattga tcagtagttt gccagatttg tttcaagatt ttcaagagag tctccgacaa
     9781 tttttggaga aaacaccttt tgccgattca tttaatcaat ttttcgcttc tattgacgac
     9841 gtgacgtctg atatagctgg atttattgga aattattggg aaagtggcgc acaacggatc
     9901 agcaacattt tttcaactgt tacagctatt tttattactt tatttactgg tccaattgtc
     9961 gcttttttcc ttttgagaga tcctcaaaaa ttttatcagt cagttttagc gattgtccca
    10021 ccagcatttc gtacagattt taaaaatttg acaaagattg ctaatcaaca attgggttca
    10081 tttctaaagg ggcaaatcat tgcttcattt atccttggag cagtctactg ggtttgtttc
    10141 ttactgatcg gattagaatt tgctagtgtt ttagctattt ctgctgggtt gttatgtatc
    10201 attccatata ttgggccgtt tatcgccttc tttccaggtt tgatcattgc attccaagac
    10261 tctactttta tggctgtcaa atttgtcatt gtctggtttg ccgtacaact tctccacggt
    10321 gacttggtga ttcctcgagt aatgggtgat cgtttacaga tccatccgat tacgatctta
    10381 atcgttttac ttgtcatggg tgatctaatg ggaattgtgg gcgttatttt tggtattcct
    10441 atctatacgt tagtcaaatt actcgttact tttattttcc gtaaattcaa acaacgctac
    10501 aataaatttt atggcgataa aggagagtat gaaaactctt cattttctga agaagattat
    10561 tttaaataaa acacaaaacg tcgatcttac gagaatagct cgtaggaacg acgttttgtg
    10621 tttttgtttt aatttttcag caaagcaatt gcctggtatg gttttagctc cagactttga
    10681 tgaagaacag tatcggaata attatcgata agaatattgg cttctgtgaa tccttctggg
    10741 atggcgatcc ttgtatcttt atcatagaaa tttgttaata caagcagctg ctgtccttca
    10801 aattggcgaa gaaagccata aacttccgga tgatttgctt cataagcatg ataatctcct
    10861 tgtgcaacga ttggatatgt ttttctcagc cgaatcaatt tcttgtaata ggacaaaata
    10921 gaatcttcag aagatagttc tttttctaca ttaatctctt ctagcttgcc tccaactttt
    10981 aaccaaggtt tccctgtagt aaatcctgct tgttctgtgt ctgaccattg catgggtgtt
    11041 cgagagttat cgcgagattt tgctttgatc cgacgaaacg cttctttttc tgtgaatcct
    11101 tgttcaagga gtaacttata agcattcaaa ctttctacgt caatatagtc ggaaatcgat
    11161 gaatagtctg ggtcgatcat gccgatttct tctcccatat aaatataggg cgtcccacgg
    11221 ttcaaatgga tcatggttgc tagcatctct gctccttgtt ttcgatactg atcgatagct
    11281 acaaatcgat tgagcgcacg tggctgatca tgattattaa aaaatagagc attccagccg
    11341 ttaccctcac tcatcttttc tccccatgta tgaaatagtc gctttaattc tgcaaaatca
    11401 aatggaggag tagaccattt attcccattt tcataatcga ctttcaaatg atggaaatta
    11461 aagaccattg ataattcttc tctactaggt tctgtataca agatacaatt ttcgatcgtc
    11521 gtagagctca tttcaccgac tgtgatgata gtggaatccg tcccaaacgt tgcttggttc
    11581 aattctttta gatacgtatg ggtgattggt ttgtccgtat attcaggttt gccatcattt
    11641 tctttattat ttttcaattg ttcgtcttta ccgataacat tgataacatc gaaacggaat
    11701 ccttttaccc ctttttccat ccaaaaacga acaactttga atagttcctc tcttactgct
    11761 tggttccgcc agttcaagtc agcttgtgtc cgatcatata agtgcaaata atatttcccc
    11821 gtatctccaa atggcgacca agcacttcca ccaaattttg atacccaatc cgttggctct
    11881 tctcttaaaa tgaagaagtc ctgataatgc tggtcccctg ctagtgcttt tttgaaccat
    11941 tcatgttctg tcgaaacgtg attcagcacc atatcgagca tgatttctat ccggtatttc
    12001 tttgcttcgg ctattagctg ttcgaattcc tccatcgtac caaagatagg atcgatggct
    12061 gtgtaatcag agatatcata accgttgtct ttttgagggc ttggataaat agggtttagc
    12121 caaatcatgt cgatgcctag atcattcaga tacgggagtt tttctttgat tccttttaaa
    12181 tctccaattc catcaccatt actgtctttg taagatttgg gatagatttg gtaaatgact
    12241 ttgtctttaa atgtcatgga tatttacctc cattcaactg atttattacc taccacaact
    12301 tatatataca agttatcatt tattattata actgaaccga aagcgtttgt aaaatttatc
    12361 tttatacatt ctaagaggtc tgttgcccaa taaaaaacaa gagatcagac atgtatttgt
    12421 ctatcctctt gtttaaaata gcaaaatatt cttgtttctt ctataaatgg tgttcaaaag
    12481 cttttatcta cggcaatcag tcttctcaaa tgtaaaacat gaggagctaa ttttatttga
    12541 gtattctttt tgctcgatgc aggacggctt ttttacaacc tctgattcat ggtgttcaat
    12601 cagctgtcct tcggtattag ctcaaaaaac ggcaaaatat gaaaaactat tttgccgttt
    12661 gtttctctaa tactcaggtc aaaccgctga tttcacaacc tctgttttta ttcattcgat
    12721 ttcaacgcat caatcatatc gatatgcttt aatttgaaat gggtaacaat catcacaata
    12781 accgtaaata cgatagttaa tcctgctgat aagacataag cccaccatgt gatcactaat
    12841 ggaaagatga cattctccat agacgcttgt tgcaagatga agtcagtcaa tatatatcct
    12901 acaccaaatc ctccgatgat tcctaaaaga gtaaagatga tattttctcg aacaatgtac
    12961 atcgtgactt ctttatcaaa aaatccaagg acttttattg tagataattc gcgtacacgc
    13021 tcggaaatat tgatattagt tagattatac aagacgataa aagccagcag tcctgataat
    13081 acgacaaaga ttaagacaac cgagtctaag ttcgccattg attcttcttg cgtttcgatt
    13141 tgtttggaca tgaaagacgt atttgtcaca gcgtctgtgg caagcagttt ctcggaaagc
    13201 atttcttctt cttttgttgt catcttatct gtacgcagca aaaatgcatt atccatcatc
    13261 gattgatcag taactgaagc ataatattca ggggacatat aaataaagct acctagatag
    13321 tttttcacga tttcttttac ttcgactttc cattcttttt gatcttcatc ataaagcgaa
    13381 atatgatctc ctgtagataa gtcaaatagt tctgctactt tttgcgtaat agcgattcct
    13441 gttttaggca aggagcgtgg gttctcttca agatcagtta aatggataaa acgatccaaa
    13501 tcagctgttt tacttggtac gatcactgtc agactttgcg aagcatgtcc tttttttctc
    13561 aattctacgg tttgagtatg aatagctagg atctcattta ctttttcttg cttctgcagc
    13621 atatccgctg ctttttgacg actatcttcc gtggatgaat ccaatgtaac gattgcttga
    13681 tagtcaatga tcggtccaaa ttgtttggct gaaacagaac ttagagaatc tttcaatccc
    13741 acgcctgcaa ccatcagccc ggcacatcca gcaattccga taatcgccat aagcattctt
    13801 gacttatatc ggaagagatt tcggtaactg attttttgat taaagctaag gcgtgaccac
    13861 agcggagtga tgtattcaag aaaaatgcgt ttccctggct tcggtgcttt cggttgaagc
    13921 aatgcagcag gccgttctct taaatctttg aataaaacga tcattgcagc gccaaatgcg
    13981 gcaatgaaga atgcggcagc tgcttgtaaa atcggcggcc agtcataaaa aataagcgca
    14041 ctcccaatat cataccgctc gttagacagc tcaaaaatga tccttggcaa aagattcgtt
    14101 cctaatatcg ttcctaaaac aatacctatc cctgaggcta gacttgcata gattgcatat
    14161 ttttttgcga tttcaacttt cgtataacct aacgccttta atgtcccgat ttccctgcgg
    14221 ttttcttcta ccatacgtgt cattgtcgta aaggtgatca atgcggcaat aaagaaaaag
    14281 aaaacaggaa aaacattggc aatcgcagcg atacgatcag acagatcacc aaattcttga
    14341 aaaccgacat tttctgaacg ttcattgtat aggtatgatg gtttagttaa attttcaatt
    14401 ttttctgcat ttcgattcaa ttcttcctct gcagaggcca gttcacgttc tttctcaact
    14461 aattgccttt cttgttcagc aagttgagct tcttgttgag ataatgtttg aacagcgatt
    14521 gttcgttgcg cttcaggaag ctgctggata gttgctgact gttgcgctaa ctgtttttta
    14581 gcatcttcta accgtgttct tgcttgcgtt gtttgtgctt tgccatccgc tattttttgc
    14641 tgtgccggtt ctaattcttc gttggccgat tttttcagct cttctaagcg ttcttcgggt
    14701 cgatcttgta aatacttttc tgccttttct tgattttgct ccattttatc tttgtaagct
    14761 ttgctgtacg tattcaaatt ctttacgttt gtaaaatcaa gatataagac agaataaata
    14821 tctgttttaa aaacggattc aggtaaatag acaaaataat cgacgctacc atttccgacg
    14881 ttcgcgtatc ctctctccaa attgttgatg aacaatggag aacggacaaa gcccgtgatg
    14941 gtatactctt tttctttcaa ttcatctgaa tcaatctgat aaatatcacc gattttatac
    15001 ccttcatctt ccgctttttc atccaataca agctggtttg gtttttccgg gagttttcca
    15061 tcagttaatt gtagcttgtt ctgcttgtta tttttgttat aagaaaatat ttgaatcact
    15121 tcgtttttgc cagtattgga ataatagatc tgttttcctg tttctacttt tgcatctggg
    15181 atcttttctg cttgagcaat atctttttgt gtcaagccac cagtagaaac gatttgcaaa
    15241 tcagaaagtc ctgctgtacc aacatagtga tccatcgttt tttgcatgac aggtccagca
    15301 gttttgacac ctacaaataa aagtgttcct agcatgacaa taaggatgat tgcaatgaat
    15361 cgtcctttag attgccgaat ttcgcgaaaa cctgctttaa gatatgttct tgttttcata
    15421 aaatccccct accatacaat ctcatcaatg gatgaaggat gatcatttaa ttcaacagac
    15481 cttactttag catcattaat gtgaatgact ctatcagcga tgggcgctaa agcagaatta
    15541 tgtgtaatga tcaaaacggt attgccgtgt tgtctgctag cgttttgcaa taatttcaat
    15601 acttgcttgc ccgtttcaaa atccagcgcg cctgtcggtt catcacataa aagaagtttg
    15661 ggatttttag ctagtgcacg agcaattgaa accctctgct gttcgcctcc tgataattgg
    15721 gacggaaagt tattcaatcg atgagctagg cccacttgcc tcaatacctc tacaggatca
    15781 agcgcatcag gagaaacttc cgttgccaat tccacatttt cttttgctgt tagattagga
    15841 acgagattgt agaattggaa aacgaaccct acatctgttc gtcggtaagc agtaagctgt
    15901 ttatctgaaa attgcgcaat atctgtgtca tcaataatga tctgaccttc atcaggtgta
    15961 tccatcccac ctaaaatatt aagaatcgtt gactttcctg ctccacttgg accaagtata
    16021 atcactaatt cgcctttttc aattgaaaaa gaaatatcgt catttgctgt aatggtcgtt
    16081 tctcccattt tgtatcgttt gtattcattt tttacttcga catacttcac tctgattcct
    16141 ccattttcac gatatttctc gctatatcta tgcatcgaat gcgttttctc acacttattt
    16201 attttaccac tgaatttctc tagactaaag aaaggaatcc tttaatttag ttgacaaata
    16261 aacgatttat gatttactta cataaaaaac attgttattt gaaagaagga acatcatgaa
    16321 tgatttaaag gaaaatatga tttttgattt atcaaaaact gcttttgtcg cgatcgacat
    16381 gcaaaaagag atcgtaaata acggtacact ctctccacac actgcagcat ctattctgac
    16441 aaacaatgat ttgttagtaa gaacattgaa acgtaccgct gccttgaaag ttttagtgaa
    16501 cgtagacatc tccactttcc cttatttgag tcaacaaact gatatgggtg gaatgactgc
    16561 ttctgtacct ccagaattca ctgatttact tttgaaggat tctttgaaag atacagataa
    16621 tatgttgact atcacaaaat acaatccaag tgcttttttt ggtacttctc ttgatcttca
    16681 acttcgccgg cgtggaatcg aaactatcat tttaagcggt gtagcgacaa caaacggtgt
    16741 atatgctact gcattagatg cctttcaaca cgggtaccat atcgtgcttg ctgaagatgc
    16801 ttgcagtgat cgtgacaaag aatcccatca attgttcata aaaaagatat ttccgaaaac
    16861 tgcacgagta cgctcaacga aacaaataat cgaagctatc caacaaagca aataagtact
    16921 ggcacacgtg attttcattt acttcttttt gatttcatgg gaaaataacc acaggattta
    16981 gataggagta tgaacatggt gaaaaaaaag atcacaatca aagaagtcgc taaactttcc
    17041 ggcgtttcaa tcacaactgt ctccttgata ttaaatggaa atacggagaa attcagtccg
    17101 aaaactgttc agaaagtttt agctgccaaa gaagaattag gctatcagcc tgactatttt
    17161 gctagacaaa tgattaccaa ggaaacgaaa acaattggtg tactcgttcc tgatatcacg
    17221 aatccattct tcagtacact gatgcgagga attgaggata tcttgtataa gcagaatttc
    17281 gtcacgatat tatgtaatgc tgattctgat catcaaaaag aaatcgaata tctagccgag
    17341 ctcacacgtc gaggtgtgga tggattcatt atcgcgactt ctgctgtctc tacagatgca
    17401 atcaacgaaa atttgaaaaa acaaggccgg cccttcattg ttttggatca gaaaaaatct
    17461 gaaggattta gtgatgccgt acgaacagac gattttcgcg gtggttacct agcgggcatg
    17521 cacttgctta gtctaggaca tcaaacgatc gcccttgtct atcctgaaaa tccaccggaa
    17581 aatgtccacg ctcggattga ggggtttaaa tctgctcttg atgtttacca aatccctcac
    17641 gaccaactga tcctcttacc tacacagttc tcaaaacaag gtggctacca gatcacagca
    17701 gaacttcttg attctgctgc aactggtgtg tttgctttaa atgatgaatt agcttttgga
    17761 ttatatcgcg gattagagga agctggaaaa agcatccctg aagactacag cattattggc
    17821 tatgataata tagatatgtg cgagtatatc aagccgaaac tgacaacgat cgcacaacca
    17881 atcttcgaat tgggacaaac atctgccaag ttattgttag atcggatcca attcccagaa
    17941 aaagaatggg aagaaaaaag attacctgta cgattagaaa aaagattttc aactgcaccc
    18001 ttgaaatagc tttcaaggtg tgctatattt ttatcgaact attaaaacgt tttactaaaa
    18061 cgttttaaaa aataattttc ccatcaaaat aaatgagata gttggaggtt tgtaaaatga
    18121 acacggttac agtaattgga agtattaata tggacactac ccttcgttta accaacatgc
    18181 ccaagccagg cgaaacgatg catgcgcatg agatttttca cgctggcgga ggtaaaggtg
    18241 ccaaccaagc agtggcagcc aaacgttcag gcgcacggac atcttttatt ggaggcgttg
    18301 gggctgacag cgaaggacaa cagttattag atctactgac aaaagaaaac atcgatacat
    18361 ctgggatagc tgaaatccaa ggcgccacaa ccggacaagc aatgattatg gtagacgcag
    18421 caggagaaaa tagtatcttg atccatgcag gggcaaataa tgctttccat gaacaagaag
    18481 tcctaaaaaa caagcaactg attacaaaca gtgactttat tattgctcag tttgaaagta
    18541 gcttagatgc gacgatccta gctttttcta ttgcaaaaga cgcaggaaaa acaacgatcc
    18601 tgaatcctgc accagctcgt gaaacgattc ctactgaact acttgaaaag acagatatca
    18661 ttataccaaa tgaaacagaa acagaaatca ttacaggaat cagagtgaca gatcataaca
    18721 gtttggtcgc agcagccgaa aaactccatg aattaggtat tggaacagta atcattacac
    18781 taggcagtgc cggcgctttc tatcatacag aaaaagaaca cggtatcgtc cctgctttta
    18841 aagtggatgc cgttgacaca acagccgcag gtgatacatt tattggcgca ttaagcagca
    18901 cacttcagcc agaccttagt aacttgaaag aagcgatcct ttatggaaat ctagcctctt
    18961 ctgttgctgt gcagtcttat ggtgcgcaac cttctatccc ttaccgcgaa gctttagaac
    19021 aatcagcagt ctaaaattaa caatgagagg aagaaacaaa catgaaaaaa ggaaaagtaa
    19081 tcaattcaga tatttcacga gtaatcgcac aaatggggca ctttgataaa ttaagtatcg
    19141 gggatgcagg catgcctgtc ccctttggta ctgaaaaaat agatttggct gtctcaaacg
    19201 gtcttccaag ttttttatct gttttagaaa acgttttaga agaacttgag gtacaatcga
    19261 ttgaattggc ggaagaaata aagacaatga acccggaaat attggaaaaa atcaaattat
    19321 tgttaccaga tacaccgatt agctttatcc cacatgaaga aatgaaaaaa gagttgaata
    19381 actgtcatgc atttattcgt acaggcgaaa tgacacctta cgccaatatt atattagtga
    19441 gtggagtggt cttctaatgg aaacgacaat gaatcctgaa cagatggtac tgtccgagca
    19501 gattcatatg ctgattgaac agttgggagg aaaagacaat attgattctg tccttccatg
    19561 tgcgactcgg cttcgtatca aattgaaaaa acagccagta gccaatgcag aaaaattaaa
    19621 acagcaaatc ccttccatta agtatttcat tttactcgat agatatgcag aaataatcat
    19681 ggatcaggga ttggctcctg cttacaagga attgacgcgt caaatcgatc attcagctcc
    19741 gaaagaaaag ctaaatccga tcgaaaaact atttggtttg atatcggaaa tctttacgcc
    19801 gatcattccg ctgtttgccg gttctggtat attaaaaggt ctggttgttt tagcgactac
    19861 gattggctgg cttagtgaaa caagcggaac ttatcatatt ttgtctgctg cgtcgaatgc
    19921 cgtcttttat tttctgccaa tctttcttgc tttttctgct gccaaaacgt ttaaagtgaa
    19981 tggatatatt gcggctgtca ttgctgcagc actgattgaa ccgaatctga cctctttact
    20041 ttcagacagt ggcaaagcag ctactgattt tctggggatt cctgtagtac tgatgcagta
    20101 tacgtctact gtcatgccgg cgattttagg tattttcttt tattcttttg ttgaaaaatt
    20161 cctcaaaaaa tatattcgcg aaaatatgca attagtattt gtacctttac tttcattagt
    20221 tatcacggtt cctttgacgt taatggtatt cgggcctgcg ggtgtttacc ttggcgaagg
    20281 tttagctgcc gcaattactt ggatgatgga tattaacgga ccgatcagtg gcgcaatcat
    20341 cgctggtggt tggaatattt tggtaatatt tggtatccaa tgggctgtaa accctgtcat
    20401 gatcagtaat atctctgctt atgggttcga ccgaatcgtt ccgttgacag gagcagccaa
    20461 ttttggaatg gctggtgcag ctttaggtgt atttctgcga agtaaacgtt caaaaacccg
    20521 ttcgatctcc ggttctgctt ttgcttcgat tttacttgcg ggagtgaccg aaccaactgt
    20581 ttacggaatt gcgatcccat tgaaaaaacc atttgttgct gcttgcattg gtgcagctgc
    20641 tggtggagct gtaatgggat ttgcccaagt gaaagcgatc gcttttgtat ttggtagtct
    20701 gaccacattg cctgcattca tttccggtac ttttttctgg tacttagcag gactggctgt
    20761 tagtctagtc gtagcaatga tcactactct cgttagcggg tttgatgaag acctaatgtc
    20821 ttatgaatag ttaatgaaaa agactgaagt atgagatgtt tatcacatat ttcagtcttt
    20881 ttctttttag gatcattatt tttataaatt tttgtgatac tttccaaact ttggtataat
    20941 ttacatatgc ataaccattt ttatccccac ttgtttatcc ccaaacaaag gtggtctatg
    21001 caataaaatg aacgtcaaag aaaaacgaat gaatgctcgt tgcttattta caaattgttt
    21061 tatttagaat acacagacag atttatgaaa agaaaaagct tttcgacacg attatctgtt
    21121 ccaaaaaatg tctaatgaat gctgaataag acagaaaatc aatgaaagga aaaggactag
    21181 tttattatga aaaataaaaa aacgatacgt ttgctagctg ctacttggct gattgctccg
    21241 tctgttttgt cgaccaatac cgtatttgcc gtagataatc aaatagctga tcaaacagtc
    21301 acaacagata cgactacatc cagctcagct actagtgaaa caacggattc ttttacagaa
    21361 gacccaactg gaacaactaa ctcaacaaca ggatcgaccg agccaactga aattgatccg
    21421 gctgatccat cagaaaatga aaagccagcc gagacatatt cccttacaat tgctccttct
    21481 ttgattggta agatccaact gactgcttcg acagcggatt cccctgaaca gcaacctttg
    21541 gatattacgg aaagtggaac ggtagaaggt ttattagcgg aaacgaaggt aacttacact
    21601 attacgccag ctgaaggtga acaaattgct tcagtcgctg ttaatggtgc caaagaagta
    21661 aattatgatg caaagacctt tacagtaggt acgaacaatt tatctattac tgcagaattt
    21721 aaagctggac cggatacttc aacagagaaa ccaactgatc ctagtgaaga agtccctagt
    21781 acaccagatc cggaaacgcc aaacaaacca gatgtgggta ataacaacaa taataatggt
    21841 tcaaataatt caaacaataa taacaataat tcgaacagcg ggaattcttc gaatcaaaca
    21901 aacccatctg gatcgaataa caatggaaat actcagagag aaccaaacca tccaaacaat
    21961 ggttcatcga atggcggaac gaacaatgct tcatctcgtc caaatacagc aaatcaaagc
    22021 ggtatagaga atccatcttc tgcttcttct gattttgttg taaaatcacc aattgatgct
    22081 gtattgccta caaatacaac gaatgtccaa caagcaatcg ttcgagaagc attcaagcat
    22141 cttggaaaac catatgtatg gggagcaaaa ggtccaaata cttttgactg ttctggtttg
    22201 acttattacg tatatatgaa agcaaccggt cattatatcg gtggctggac aggcgaacag
    22261 caatatgcag gtacgcagat tccagtaagt caggcccaac ctggcgacct cgtcttctgg
    22321 ggaccttctt ccggtgttac tcaccatgta ggtatctata ttgggaacgg acaatttatc
    22381 catgcgcctc aaccaggaga taaagttcga gtaacttcta tttcagattt cacgcctgat
    22441 tttgctgtcc gagtaaactt agcaggttta cctgctgctt caggttcgct gacaggcggc
    22501 tcatctattt tagatggatt agatggctcg ttccatttca gtcaaaacca aacaactgat
    22561 cagttcttga aaaagattgc agatgatgca caggaaatcg ggcaaaaaga aggaatctac
    22621 gcatcagtca tgatggctca agctatcctt gaaagtggat ctggaaacag cttattatca
    22681 agcgaaccaa atcataacct ttttggtatc aaaggatcat ataaaggaag cagcgtgacc
    22741 ttcaatacac ttgaacaaga tagttccgga caaagctacc aaatccgcgc acaattccgt
    22801 aaatatcctt cttacaagga aagtttggaa gactatgccg acttgatcaa aaatggactg
    22861 actggcaatc ctgatttcta caaaccaact tggaagtcgg aaacaaaaga ctacaaagaa
    22921 gcaaccaaat atttggaagg acgttatgct acagatcgcc aatattcaca aaaattgaac
    22981 gcgatcatcg aagcgtacga cctgacaaaa tatgatgaac cgaaaaaaga agcagcaaca
    23041 actgaagaag caaacgctga aatcctcaat atgtctaagc gattcgatgt gccaatcaaa
    23101 tggacaacag atggcgtgcc aatcacagga ttagaatttt caacgataaa acgcgtcaac
    23161 aacgcttctt ccatcttccg tgtcgcttca atctgggatt tgtggaacaa ttttacggca
    23221 agacagatac cagaaacaac tactcgtaca gttaaagctg caactgttcc tgtactaacc
    23281 ttgttaagtt tagacaaatc attctggctt gatctgcctt taaagaaata gaataaatta
    23341 aaaaggggat gtgacactgt cacactccct ttttaattta ttctatttct ttatatttat
    23401 tttgtattcc gttacaaact cgcgtacttg gttaggttcc agagtgacat ctccaaaact
    23461 ttcaagtcgc tccgctcctg gcagttcttg agtctcaaat gtgataccgc catgatttac
    23521 taatttattc ccgcgcattt ccggtccatc ttctccaaaa tttgctgtaa aaatcacgat
    23581 tcctgaagca tctgttttta cttggatcga tactttttca tctgggcttg tcaattccgc
    23641 agcatttagt aaaccagatt gttttttcaa caaaaatgga tgatcgatgc cgcccactat
    23701 cattgattgg cgaaaatcac tggcaaagac ctcacctaat tttttaggac gttggaagtc
    23761 aaatgctgtg ccacttactg aaactacttc tcctgtaggc gttgtatctt tattgagcgt
    23821 agcaaataat cggctattca accaaagtat atgttcgtcg attgactgtg ctggatcacc
    23881 ggttaaattg aaatagacat gatttgtagg attgaatact gttttgcggt cactgatccc
    23941 ttggaccgtc actcgccatg tatcatcgtt cgtcagcgta taccgtactt ctacatctaa
    24001 agttccagga aatccgtttt ccatatctgg gctagtctga tagaaaatga ccgatgcttc
    24061 gttttcacca ttgatgactg tataatgcca ctgcttggct tcaaagctag gtgcaccacc
    24121 atgtaaacaa tgaccagtgt ccgggtctgt ttttacttcg aacgtttcat catctaattt
    24181 gaatgtgcca ttcgtaatcc gtccagccat acgtccgatt gaagcaccga tataagggtc
    24241 tttttctaga tattcttttg cttcatcaaa acctaagatc aactctcgtt tttcattatc
    24301 gattattgtt tgaaaatgaa caacccgtgc cccaagatca ctgacagaca aagatgtccc
    24361 attttgattc gtgatcgtaa tcaagtgata gttttcgcca aaattttgtt cattgatttc
    24421 catgttcctt gcaccctctt ttctttcatt tgcactgatt ccagtataca tggaaaccgt
    24481 tttcttaaca gcttattccg tgagtaacga gaaaaaattt actatattct aataaaagac
    24541 cgtgaaaaaa aggttgtgac aaaaatattg tcacaacctc tttttccgaa caaacggtgt
    24601 tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatatgag gagctatttt
    24661 tgcttggctg ttcttattac ttgatgcagg acagcttttt cacaatctct ttttcaatac
    24721 gattccagta tataaccatg tcgtcagtac atagtagatg aaatacaata cgaagaatat
    24781 cgtaaacgga acccaaatat ctttgtatgg attttgcatg aatagaccaa acatttgtaa
    24841 tccgaataag acatggacta cacctaagat tcctggtgct aagaataaca cgcctacttc
    24901 tctacggatc gattgtttaa gcaacccgcg tcgtgttcct atcttttcaa gcatttcata
    24961 acgagcaatg tcactcttgc ttcctgacaa gatcttgaac atcaaacagc tagcaagcat
    25021 ggtcaagaaa gctaaaccta ggaagaatcc cataaattca aaaccagaga atagaccatt
    25081 atacacttca tacatttcat atttctggtt gctgtactct actttttgca aagatggatt
    25141 gttcatttta ttttgagcaa caagtgtttc gattggtttc aggtttgctt ggaagtcttt
    25201 tacctgaact agctgtaaag tggattgtgg caagttcagt tgatcaaatt ctgattgact
    25261 gactaccttg atttcttttt cttgctgttc cggcaattca tagcttcgaa gttcctcttg
    25321 tacagtcagt tcattcattt ttttagcagg aactttttct ttcgtgatct tcggcggtgt
    25381 tccatcttca tgttttatca tgatcaatgg ttgagccgca aattcacttg cattatagta
    25441 aatcgttccc gcatcttctt tttgagtata agaagcagaa agtgttggtg aaagttcttt
    25501 gattttttgc tgatcttctg cccgaggatt attcatcact agatcatatc cagaattgtt
    25561 ttctgtcaag tctgcaattt catttttaaa acctaatcct accgtcaaag cgcccagtgc
    25621 caaggcaaac agcatagcga ccattgagag catttgtgta tattcacgaa tacgaaagtt
    25681 taactgtgac aatgtgaaat tgttcaattt tttcaatgaa atctgttctg atttctttag
    25741 taatgataaa aagaaaatga tcacagaatg gaaaatgaaa taagttccta atacgatcgt
    25801 aactaaggct acccctatac caaagacttg ataggtcccc acgtgtttca tcatatagta
    25861 accgcagcct agaaaagcaa ttcccagtac tacttcaagg aaaaacaaga atttatgctg
    25921 cttgattctt gttggtgtct gatccgcacg aatcaatgct aaaatcggtt ttttgacaat
    25981 cgaaaaagca ttgacaacag cagctaataa aaacaaaaca gcaaaaaaca aaacagtgat
    26041 caacaacccg tgaatactaa aaggtgtaaa atgcgaaata ttgagatcta actgatccac
    26101 taataatcgg tggacgatac ttgtcaatcc tacaccgaca gctgaaccca ctaaagtagc
    26161 tgcgatccct actaagaatg tttcagaaaa aatcatctgt gcgatttttc tgccttttgc
    26221 tcccagcatc ataaacattg catagtcttt ttgccgcatc gtcatcaaaa aggaatttgc
    26281 atataaaata taaacgaatg tgatgatccc tagtaaaaca gaaccgaatc ggaaaatgat
    26341 aactgtagcc gcaacgatcg tattactttt caaaaaagct tcgttgcttg ccattgattc
    26401 aaacatgtaa aaaatagcag atgccataac taatcctgag aataacacca agtagtctct
    26461 taaacggctc ttgatcccag taagtgataa tttccaaagc atagtcaatc tccccctact
    26521 gttccaagtt tccaagaata gatagtattt ctcgatagaa ggaatcacgg ctttgatctc
    26581 cacgtttcac ttcttgatgg ataccgccat ccttgatgaa taaaatacgt tggcaataac
    26641 ttgcagaaaa cggatcatgc gtcacaagaa gaatcgatac atgatctttt gtattcaatt
    26701 cgtccatcgt atctaaaaga tcccgggcac ttttggaatc cagcgcacct gtaggctcat
    26761 cccctaatag aatcgttggt tgtgtgatca atgcacgtgc ggcggcgacc cgttgttttt
    26821 gtccaccgga aatctcagaa ggatagcttt ccaaaatatg agagatggat aaacgttctg
    26881 caattttttg gacacgttgc ttgatttctt ttggtctgac gccttgtaaa gaaagaggaa
    26941 cagcgatatt ttcttgtgct gtcaagcttt ctaataaatt gaaatcttgg aaaataaagc
    27001 caatctcatt cgcacgaaaa tctgctagtt gatttccttt taatgtggtc acatcttttt
    27061 gattgattcg gatatgccca tctgttggtt tatctaatgt ggataaaata ttcaaaagtg
    27121 tcgacttacc agaaccagaa gcgcccatga tcccaataaa ttcgcctttt tctacatcga
    27181 agctgatgcc gcttaatgct tgcgtctttt tctcattgtt ttttccgtat acttttgtta
    27241 cattctgtac ttcgactatt ttgttcattt ctttccctac cctttaatta gtttttccag
    27301 tgctgttttc ggcacgtctt ttttttcgac ttcttcatac gaagtgacac ctttattttt
    27361 gttccaggtg atttttaaat atgctccttt acgaagcggt cgagtcaatt gtcccttgaa
    27421 gtccagttct ttttccttac cctctttcgt atagccaggc aggatatatc tatagttgac
    27481 tgtttcttgt ccagagtctg attttgaaac tgtttttgta ccatctgtcg taatttgtac
    27541 atagtattct tctccaccca taactacgta atctgcgatt ttcacccctg caagtaccac
    27601 aacgatgaaa acaagtagac cgatcagaag ttttttcact ttttcccctt ctctcttact
    27661 ttatatactg attatagaaa aaagcgaaca attctgcctt cgaatcaact atcattcttc
    27721 tttcaatttt gtcattttct ttaaaacaac taaaaaaaga gttcaagaca ataatccgtc
    27781 ctaaactcct tcttcctatc ttacaaaaag tatgtcactt acggtctttc atgtcgcctg
    27841 tattgataac aggttgcgtt cctttgtctg taatgattga gacaagtaaa ttattgatca
    27901 attggacttt ccgatcatcg gtgaaattga tttcttgtcc atcttcgatt tgctctaagg
    27961 ccatttgagt gattgtgaca gctccttcaa caatggtttg tcgagcagac aaaatggctt
    28021 ttgcttgttg acgttggagc atggcactag cgatttctgt tgcataggct aaatggttca
    28081 agcgtgtttc gattacttcg acacctgcta ctgctaaacg ctcttgcagc tctttttgca
    28141 actcgtcaga cactgccgtc gtgtttcctc gaagcgtcaa atcattctca ttgaatgtat
    28201 catacgggta ttggctagcg atatgccgaa tggctgtctc actttggatt tctacaaaat
    28261 cttgataata cgctacatcg aataatgcct ttgctgtatc aacgacttta aagactacta
    28321 ctgctgagat ttcgatgggg tttccatcca aatcattgac ttttaaaaca gaactattga
    28381 aattccgaac tttcaacgaa acagtcattt tttgtgccaa aggtatcgta aggaaaaagc
    28441 cattttctcg aatcgtacct aaatattgtc caaaaaacaa aattactttt gcttgattag
    28501 gactgactac agttgccgaa cttaacaaca ataacgtgat caaccaaaga aaaatactta
    28561 aaaaaagtgc ccaaactttc gcttgccaca tacctaaata gaagaaaaac aatccaacta
    28621 aagctaatac gaccaatccg acaatccctg cataaccatt gatataaaat gctctttttt
    28681 ctctcatgct acttcctgcg ctcctttatc cttcaataaa tatctaatgt tactttttta
    28741 attttttttt cgaatacgcg ccattacacc ttctagttct aactctttgt cttcagtgat
    28801 gtaccaaccg cttgcctgtg tccctccggc tgcctgcggt aaataatcca attttgttcg
    28861 aacccagtgc ttttcttctg taaaatagtc ttctaccaag acctctagat ccatccctgc
    28921 tgaaagaggc aaatcttccc gaacagattt aaagataaat cgttccttct tcccatcata
    28981 cttcaatatt cctgtatcga taatcggtaa agaataataa tccagctctt tcttcaccgt
    29041 caataaatgt ttgaaaactt catggtacac ttgctctaat tggcgacctt ctgaattgtt
    29101 cgggtaatta accatcactt tttctttgtc tggatcatag aaggtttggc ttagttgagt
    29161 gatataagta tgtaatcttt ccatttctgt ttttaacttt tctctttgac cttcttctac
    29221 cttcatatac tctttcaccc tgttttcttt ttagatattt tctttcttat tttactatag
    29281 ttatgtgcgt taagcgatga aaaacagcaa agattggaag gatctttgct gttttttctt
    29341 aaaatatcca gttgagtaca ggatcaatct gtttcattgt cctcttgatc cgtcgtaata
    29401 ttgatcgatg tgtctttagc agtgatcttt agctttttct gtccttgttc taacaaagta
    29461 tttgttattg gctggtagtc tttttcaata taagattcac cagacgtttg gatagtatag
    29521 ccactgtttt ccatctcatg caaagataaa ttgccaccgt ttaggatgac ttttccttct
    29581 ttgctgatca aactgtcttc tagatggaag ttgctgtttt cacctgtgac gatcaagtta
    29641 tccacattgg cctcatctaa tcgaagtgag cctaattcat ctttcgcagt tattgttcct
    29701 cctgtaaatt cattgaacga tacccagcca tcatgtgtct tagtattcac ttttttgatt
    29761 gatacttcgt tgatatcaat tgcactatta ttcccttcga ttgtcaattc agataattca
    29821 tgatctgcag ggacaacaat agtgatttgt gcacttgtgt cttctaaact aatgattgca
    29881 cctgttgatt ttttgactgc attcacagtc aacacgccat ttttctcctt tatttctggc
    29941 ttttctgtct tatcataagt cgtttttatc tcaaaatcca atccgcgtat gaccgaaact
    30001 gtttgatttt ctgcatggat gacgattttg tttgcatcct tgattttttt tgtctcagta
    30061 accatatcga tcaaccgagg accattatcc caagtcagac tcttttgagc gcctaatgta
    30121 taagttgcac ctgctaggag gccaccagta agcatcagac caataccggt aatcaaaaaa
    30181 tgtttcaatc tcaatttatg tgtcctcctt caaacgcccg ctgaatgcag atctcttttt
    30241 gtaataaaac gtcgtcccat ccatttaaca aagttcatca tgacataaaa aagccctttt
    30301 gttcctttca gcaacagtgg cgaaataaat actgcgcttc ctgtggcgaa cagaccgaca
    30361 ccgataaaga ataatccact cagaatcgat tgtccaaaca cactgattcc tgagatgatc
    30421 atgaataggc caacacctaa gatactgata cataaaacat agatactaaa aatgagcgct
    30481 agtaaagcaa ccaatagtcc cccaataacc aatataaacc ccagtgctag tggaatgagg
    30541 acaggagaag caaataaacc gataatgatt aaccaaatca ggcgaatttg tctttttgga
    30601 tttttcacta actcgttttc tgcttgttct tcttctgaaa agtaactgat ttttaatttt
    30661 aaggcaaact gctttggtgt accatattca tcaatgatct gatctaatgt ttttccagaa
    30721 tctaaaaaat attcctcata atactggatc aaatcaaaac gatcgtcttc aggaatttct
    30781 tgaagatgat ccacaacttc attcaaatat tcattcattt gtgctctcct ccaataaccg
    30841 gtctaaagaa tctcgaaaaa caatccattc atcaataatt tcttctaaat gacgacttcc
    30901 cattggtgtc aacgagtaat atcgacggtt tctgccttca aaaggttcat cataggtctc
    30961 aagatatcct tcttttttca atcttctcaa aaccggatat agtgtagatt cactgacaga
    31021 gagactttct tgtacgattt ttgttaaggc atacccatac atatcttcct tgcttaatgc
    31081 tgctagcaca gtaccatcaa gaagaacagt tggaatctgg atgtgcatta cgtcaccttc
    31141 tattctcaag tgtaatattt tatttcaatc tatattatac aacatataat atagtttcaa
    31201 aatcagtttg tcaaatacaa aaaaactgac taaaagattt gatccttttt agtcagcaaa
    31261 aaatggcttc tctttgattt ttattataac agtggcataa tccaatttgt ccacatccag
    31321 ctcagtggca tgataacgat cattgcaggt atcatgtcag ctactgggaa cattttcacg
    31381 ttgatcattc taaaaccggt agcgaccatc aagaatccgc cgcaagcttt aaaatctaag
    31441 atcatgtctg gtgtagtcaa tggataaata aattttgcca ataagaacaa aatataaaaa
    31501 ataatgaatt ggggaattgc tatcacagaa acgacatacc ctaaattaca cgcaaaaata
    31561 gctgctgtga aaaaatcaag gacagattta gaaatcaaaa tcgtactgtc tcctgtcatc
    31621 ccagaatcta aacttccata gatccccgta ccacttgcac aaaatagaac aatgaccgtc
    31681 actaatgtgc tgataaattc atcgtgggtc ataccaagct tttcactagg gaagatttta
    31741 gaaataggta cttgcatcag cgcagccccc ttatttatcc cattacctaa atgaacaatc
    31801 aaaccaatcg ctgttcctat cactaaagca aaaataacag ccggcatatt tttcatcggc
    31861 gcaatggaat agattcccat ccccatcgaa cacacaccaa aaaccatcgt cagctgtgct
    31921 ttaaaatgtt cacttaattt atttcctact aatccgccaa ttagtccgcc aaacaaaacg
    31981 gaacctacat tgatcaaaac tccagttggc atcaaaaaac tcctctcttt ttctacgaag
    32041 cgaaaaaaat cagaaaaatt tctgtaatta ttgctttcgc tgcaagaaag attgtaacag
    32101 acagagaaga ttacaccaat tcattttttg tgttaaagta ttctttattg gaataagtag
    32161 gaggaatatg atggatatcc ataaattcga agtttttctt gatttagccg aaacaatgaa
    32221 tttcaccaaa actgctgatc gacaattcac cacccaagga aatatttcta aacagattct
    32281 ttcgttagaa acagaattag acgtcaaatt attcgaacgc gcccatagaa aaatcgaatt
    32341 gaccgaagct ggaagcttat tgctcccata tgtaaaaaat gtcgtagaac aataccatgc
    32401 tatgcaagaa gtgcttgaaa cctatacgaa agacaacaat ctttcattaa atatcttaac
    32461 gataccgaca atgatcaatt ataaaggttt ttccaaaatc accgagtttt tgaaacgcca
    32521 tcctgaattt acagtccagt tgaaagaagt ggaaagtgtc gaattaactt acaatgacgc
    32581 caatgaacat agtgacacga tccactttgc tagaagtttc caggccccac caagttcgat
    32641 tgagtggttg cctactgaaa cagacgattt tgtagcagtt ctacctaaaa atcatccttt
    32701 agctagtaaa aagaaattgg atctaagcga actgaaaaag gagaactttt tgctccttgg
    32761 ccccactacg aacttgtatc aacctgtact agatctgtgt cgcgaagctg gatttgaacc
    32821 aaaaatctct tataaaggag caagaatcga cttgatcatc aatatgattg caaatgacat
    32881 ggggattgct attgttatgg aaaaaacagt caagaatcta ttaaaagaaa atactgtgat
    32941 tctccctatt tctccaacaa cagagagtta tcttgcattt actcgtaaag tcggtgagca
    33001 ttctttggct tcagatacat tttgggagta tttaaatcaa gaagaaaact aagccctaca
    33061 ttttctgtcc atctatccac ccaaaaaaag agagcgacgc atgactttcg ctctctttct
    33121 tgcatcatgt ccttgtttca atgacacctc taacatttct cgtagttcaa taagaaagca
    33181 gcgattttta tgccaaaaga accgtaagaa catttcccta atcacaaaaa aacgactaaa
    33241 aacccctaaa aaccttcaat agaattttta ggggttttat tctttttcct attgtttact
    33301 taaccgtatc attgctgtcg ttcctactcc ttcgatactg ctataatcaa tacgtccatc
    33361 atgcagttct agaatttttt taaccagtga aagtcctagc ccattcccag aaatttgacg
    33421 acttgtatct ccttgataaa atttttcaaa cattcgatct ctggtctctt cattcattcc
    33481 tacacctgaa tcggtcactt ccagtacaat ctctgtggct gtctcaaata gtttgatatg
    33541 aatctgtcca ttgatttgat tgtactttat ggcgttatcc atgatattca gccacacttg
    33601 gtacaaaaac tcttcattgc ctgtgtaatt tactgcagca agttcgatat ccaattctag
    33661 ctgttctttc tcccatttag gctgaaaaaa aacgattact tctctgattt gttcatctaa
    33721 tcgatactct ttcttttcca gttggatacg ctggttttcc agtttgttca attttaagat
    33781 gttctctgtt aattgtgaaa gctgcgtgat actttcgata atccgatata aaaatatttg
    33841 gcgttcttcg tcagagagat ttggggcttg taacaattga acatatcctt ggattgttgc
    33901 tagtggtgtt ttgaattcat gggatacact ggacacaaag tcattcctca gtgtctcgat
    33961 actattgagt tcttggacca taacgttgaa atctttatac agttgctgta cttctgctac
    34021 tttctgctgt tcatccattc gaatcgaaaa atctcccgtt gctaccagac tcatatttgt
    34081 ccctagcgca ctgattggat gcaggatacg ccgcccaaca aaaatcgata ttcctgtacc
    34141 cacgaccata ctgaaccccg caaagacgaa taacgggtat aacgttggtt ttccagccga
    34201 ttcagcaggc gagatagaat gtcgagacaa taagaaaata aaaccgagaa aacacagcag
    34261 tgtcactaag atcgaaatga agacaatact agcaaaatag atccataatt gtgaaaatag
    34321 cttttttttc atctttaaat aaccgcctta tagcctagtc cccgtactgt gatgatttga
    34381 aaatcttccg tcttttcaaa acgggtcctt aaacgtttga tatgtacatc gatcgttcgt
    34441 tcttctgtat tcgtatctaa tccccaaata tcatccatca actgttggcg agtgaagatc
    34501 ttgttgggat aagacagtaa tttatacaga agcagaaatt ctttttgagg cagaatcaac
    34561 gaatgctgat cttgtaacac tgtataagca tctagctcta attttgtttg tccgattatc
    34621 aagctactgc tgtaattgat ctttgaacgt cgaagcaatg cttccacacg taggatcatc
    34681 tcattgacat cgatgggttt tgtcatataa tcatctgctc ctaatttgaa cccgcgtttc
    34741 ttatcctcaa aatcggcctt tgccgtaatc attagaacag gtgtgtcaaa tctggtgcta
    34801 cgtaatgagt ctagcaagtc atagccatcc attttcggca tcatgatatc tgtaatgatt
    34861 aattctatac gtgtcgcttc tagttttttc agcgcatcct ctccattcaa tgcggtgatg
    34921 actgaaaagc ccactcgttc caacacagaa cggtaaagca actgtaattg cggatcatct
    34981 tctactacta aaatacgatt cattgatctc cctctgtttt ttgaaaaatt tctagatttt
    35041 ttcttgtagt tcatcttcag ttaacattta tttcgtatac tacgatcatt gttctgatat
    35101 acttattagt ataccgaata tttcaatacg ggagaagggt gattatgctt caattaaaag
    35161 atataaaaaa atattataaa gtcggcgaaa ccaccaccaa agcattggat ggtgtgtctg
    35221 tcgctttcag acaaaaagaa ttcgtagcga ttttaggtcc tagtggttct ggtaaaacga
    35281 ctatgctgaa tgtgattggt ggactagata attatgattc cggtgatatg gtaatcaacg
    35341 gaaagtctac taaagacttt aaggacagtg actgggatgc ctaccgaaac aactctatcg
    35401 gtttcatttt tcaaagttac aacttgattg gtcatctagg cattattgaa aacgttgaac
    35461 ttgggatgac attaagtggt gtttctaaag acgaaaaacg aaaacgggca gaagatgcat
    35521 tgcaccgagt cggcttgaca aatcatatgc ataaaaagcc aaatcagtta tctggtggac
    35581 aaatgcagcg agtggccatc gctcgagcac tagccaatga cccagatatc cttttgtgcg
    35641 atgaaccaac cggagcgtta gatacagaaa caagtatcca gatcatggaa ttgatccaag
    35701 agttatcgaa agaaaaatta gtgatcatgg tcacacataa tcctgaactg gcccatcaat
    35761 acgcagatcg aatcattgaa ttttctgatg gtaagatctt aacagattcc catcctcaca
    35821 tcgaacgacc aaaagatgat caatttaact tacgcagaac aaagatgagt ttttggacag
    35881 cgttaaagct ttccttcaac aatatccgaa caaaaaaagg acgtactttc ttgacctcct
    35941 ttgcttccag tattgggatc atcggaatcg cgatcgttct gtccctttct tctggattcc
    36001 aaaaacaaat cgataacacg caagctgaaa cgatggcgaa atttccgatt actatttcta
    36061 aggtaacgac taaccagact cgtgacgatg ccggattggg tgcaagcaaa gcggattatc
    36121 cagacagcaa aacgattact gcaaaagtca gcgaagaaga taaagcacag cacacgaata
    36181 agattgatca aacatacgtc gattacgtaa cagacatcga tcctaactta agtaataata
    36241 tcggatttac ccgtactaca ggaatcaatc ttttacgtga tgtaaatggc aaagtacagc
    36301 ctgtcagctt ctcgaatcaa aatcctgata cggaatctct ttctttatct agtacgatgt
    36361 cagcaatgac aggtgtggga gtgtcaagtt tccctacaca gcttgacact agcaaagaaa
    36421 actttttaaa agacaattat tctttacttg ccggaagtta tccagcctca gcaacagatg
    36481 tcgtgctgat cgtagacggc aataataata ctaatatcaa cgcattgaag aatcttggat
    36541 ttgatgtgaa agaagacgaa aaacttgatt ttgatgatat cgttggtaca acattcaaat
    36601 tagtaaacaa caatacttac tatacaaaac ttccaactgg gaattttatc cctaatacag
    36661 attatgatgc gatgtatcag aatgcttctg atgaattgaa gatctcgggg atcctacgcg
    36721 tgaaatcttc ttctacaatg aatctcttat ctccaggaat cgcttatagc gatcaattaa
    36781 cgactcaaat cgtcaacgaa aacaaagaat cagaaatcgt caaagctcaa agagacagtg
    36841 gtgtaaatgt cttaactact gaaaaagttg acgaaaatgc caaacaaaca ttgcttagtt
    36901 atttaggtgg agacagcttg ccttcaagta tcatgattta tccgaacaat tttgaagaca
    36961 aagaaaagat cttggattat ctagatgatt acaataaagg caaatcagat gaagataaaa
    37021 tcatttatac tgatttagct ggaacgatga ctgaactgac tggcggattg atggatgcta
    37081 tcacttatgt ccttattgct tttgcaggga tctctttagt aacaagtatg atcatgatca
    37141 gtatcattac ttatacatct gtgattgaac ggacaaaaga aatcggtgta ctaaaagctt
    37201 taggtgcacg taaaaaagac atcactcgcg tatttgacgc agaaacgtgt attttaggta
    37261 tttcttctgg gatattaggt gtgtttattg catggctagc tacgttccct atcaatagca
    37321 tcttatacaa catgacggat ctaaaaaatg ttgctcaact aaatcctgtc catgcgatta
    37381 ttctagtgat cgtctctact atattgacga tgcttggcgg tcatcttcct gcacgaatgg
    37441 cagcgaaaaa agacgccgct atcgctttgc gtgcagaata aggttgtgac aaccacattt
    37501 agatttgagt cataagagcg ctgaaatagt agaaaacagc aagaaacgag gaagtaatat
    37561 gttccttgtt tcgcgctgtt ttttttaatt attttttata attctggtat acttttcttt
    37621 cctttgtacc acaactcttc tggcgtatac ctgtcttttc catttatgaa tactgttcca
    37681 tctgcttcaa tcgttacttc agtcccaatc cacatattac gagaaagtgc gagaagattt
    37741 tttctttttc ctcttccttt ttttccaaat tgcaaggcat cggttatctc ttctgtccaa
    37801 tgagatttca cgtattttac ttctccgttt tcaaatgtaa ctttcaaaat attcgctata
    37861 ttttccactt ttactgcttt aggaaatgac tcctccattt ttttcacttt ccttcctcca
    37921 acactaagta tgcgctttct tttagtgata agtatatcat agtttattct attatcaaaa
    37981 aaacgcatcc attccattgt agaatacttt accgaacaag ttgaattgtt ttcctctttt
    38041 tcacctttta ctatagatag gtaaagacaa ctaaggagga atctctcatg acagatcaac
    38101 cgtatgattt aagaaaagtc atcgaagaac taaaaacact tccaggtcaa tatcacgaaa
    38161 cagatattga aatcgatcct gaagcagaat tatctggtgt ttaccgttat atcggtgctg
    38221 gcggtacagt aaaacgtccg actcaagaag gaccagctat gactttcaac aatatcaaag
    38281 gtttccccgg cacacgtgtg aacatcggaa caatggctag tcgtaaacgt gtaggtgaaa
    38341 ttttgcacca tgattatcga acacttggtc gtttcttgaa agacgctgta gaaaatccaa
    38401 tcaaacctgt gaaagtagac aaagcagacg ctccagcaca agaagtcgtt catctagcta
    38461 ccgatgcaga cttcgatatt cgcaacattt tgcctgcacc tacaaatact cctgaagatg
    38521 ctggtccata catcacaatg ggtgtagtct taggatcaga tccagaaaaa acaatgagcg
    38581 atgtaacgat ccatcgtatg gttctagaag ataaagatac gatcggtatg tacatcatgc
    38641 ctggcggacg ccacattggt cacttccaaa aacaattcga agccttgaat aaaccaatgc
    38701 ctatcactat caatattggt ttagatccag caattgccat tggcacgact ttcgaaccac
    38761 ctaccactcc acttggctac aatgaactat ggtgtgcagg agctttgcgt aacgaaccta
    38821 ttcaaatcgt tgatggtata gcagtcgatg aagtgggtat tgctcgtgct gaattcatca
    38881 ttgaagcaga aatcatgcca aatgaaacaa tgcaagaaga tatcaacacg aactcaggaa
    38941 aagcaatgcc agaatttcct ggctatactg gggatgctaa tccagctgta caagtagtga
    39001 aagtcaaagc agttactcac cgaaaagaca atccaatcat gcaaacaacg attggtccca
    39061 gtgaagaaca cgtatctatg gccggtattc ctacagaagc aagtattctt gccttggtcg
    39121 ataaagctat tccaggaaaa gtagtaaatg tctacaatcc tcctgcaggt ggtggtaaat
    39181 tgatgaccat catgcaaatc cgtaaagaaa acgaagcgga tgaaggtatt cagcgtcaag
    39241 cagctattct tgctttatca gccttcaaag aattgaagac agtcatttta gtcgatgaag
    39301 atgttgatat tttcgacatg aacgatgtag tttggacttt caacactcgt ttccaaggag
    39361 ataaagatat catcgtgatc ccaggaatga ggaaccatcc attggaccca tcagaattac
    39421 cagaatataa tcctggctct attcgtttcc gtgggataag tgcaaaaact atctttgacg
    39481 gtacagttcc tttcgatatg aaagaacatt ttgtacgtgc aaaattccaa gaagtagaag
    39541 attgggaaaa atatttgaaa taatgaatga tctgcaactg ttttgacatc ataaaaagga
    39601 gtgcggaaaa tggaacaatt ggcacaacgg aagaaaaaaa tcgtgattgg tgtaagtggc
    39661 gcttcaggca ccatttatgc catcaaccta gtgaagaaac taaaagaata tccgatgata
    39721 gaaacacacg gaatcatcag tccatgggca aaacagaact taaagctgga atccgactta
    39781 tcactaactg aattcgaaag cctgtttgat taccattatt caaacaaaga tttaggtgct
    39841 gctgtcgcca gcggatcatt tttgaccgat ggaatgatca tcgtaccggc aagtatgaaa
    39901 acagtcgcag gtatctctgt tggtttaggc gacaacctga ttgcacgtgc tgctgatgta
    39961 acaatgaagg agcaacgaaa gttagtcatt gttcctcgtg aaacgccatt aaatgccatt
    40021 cacttggaaa acttgactaa attagcacga ctaggtgtgc aaatcatacc accaattcct
    40081 gctttttata atcagcctca aacgatccaa gatcttgtta atcaccatac aatgaaactt
    40141 ttggatcaat ttgaaattgt aaatgagcat tcaaaacgtt ggcagggtga ttaatatgga
    40201 aaaagaaacg atagttgaag taaccaaact aggtttcacg atccaagctg ctgttaaagt
    40261 aatcggtaaa gacttattga tcactttaac aggcggagat acaccacata tcggaacagt
    40321 cactacattt tctcgtgata cagagatgca aactgtacgt ttccctagcc acgatggtcg
    40381 gttccataaa gacgatgtct tagccgaaaa aatagctgtg atcatcaaaa ctgccgttcc
    40441 gggaagttgt acgatcactt ccggtatcca tgttgaccat atctccaatg cacaaatcaa
    40501 cgcctctttt ccgatgtcag aagagttagg ttacaagatt ttagattggt tggaaaaaat
    40561 agatttccat atcaaagaac cagtttatta taaagatggt gaaaaaccat tatgaaagaa
    40621 gaggttgtga aatcagcgtt atgacctgag tatgagagaa aaaaatggaa aaatagcttc
    40681 tcatattttg ccgtttttga gctaataccg agggacagct gattgaacgc cgtttatcaa
    40741 gaggttgtga taaagccgct tcgtttcgag tagtaagaaa aattttgtaa acatgggttt
    40801 ccacattttt gcataaattt gacttactac cgagaaactg gcttttgaac accgtttatc
    40861 aagaggttgt gacacgtctt ttgtcacaac ctcttttatt tttgcttcac tgtaactgct
    40921 acaatatcac cgatttcttt tccgatgttt ttacgaatat cttttcgtag tccaaggatg
    40981 tagcagactg aaccatctgg atttttcacg cccatattca cgatactgcc ttcatacggc
    41041 acattgtcaa aagttgcttg gactttaact cttccagcac cgaattcttt tcttatatca
    41101 tacggaaaaa taatatatgc cccgcccttg ccgacttcgg aagcatagat ttttgattca
    41161 aaagaataaa tttccctcat tttatttctc ctatttttta catctagtat agcatagcaa
    41221 aaaaaagaag gaactgtgac aaaagtcttg tcgcagttcc ttttttcgaa taaacggtgt
    41281 tctcctgcca actcctcggt cacaagtcac aatagtcaac aaacatgaga agctgttttt
    41341 tgaaattgct ccttgtgact cagagttaaa cggcaggctc acaacctctt ccttaaacgg
    41401 tgttcaaaag cttgcatcta cggcactaag cccaatcaga tgaaaaatat ggagtgctat
    41461 ttttatctga ttgttcttac tgcttgatgc agagcagctt tttcacaacc tcctacatgg
    41521 tgttcaaaat attattcgcc ttttgcttct ttcactaatt ttgcgaatgc ttgttgttgg
    41581 aattcagcag ctaaggcttt cgtacgttgt tcttgctctg ctttgctcat ttttggattt
    41641 tcagtcattg cttttaccat accgtcttga acataagctt ttgcttcact ttccatcgta
    41701 ctttgctgtt tcttcactaa ttcagctaac ttggctgctt ggtccgtact taattctacc
    41761 catgtctctg ggattttgaa tgtattcact cggctgtcat attcgtgatt gttatcagca
    41821 atattgaacc agaatttata catgtcgttt ttgtattccc aataagtagt tttcgtcact
    41881 ttttcagcat cgccgtctgt cttttctact ttatttgttt cgttatccgt accagttgct
    41941 tttggttttt cttgtttttc atttgttcgg taaagataca ctttttcttt tccattccct
    42001 aaagctttat acaaaagcat atctgctcct tgggaatcag cactggaaac aattttttct
    42061 gtttttgttt ccgttacttt ttccattcca aagtgattgg acaaattagc gacgatcaaa
    42121 cttagtgaag cgacgaagat tagtccgaaa atcaaagata aaaaggtttg ccatgttttt
    42181 ttagcgaaca cgttcgttaa agcaaaagca agcaagctga caatcaaaac tccaataatc
    42241 atgcattatc ccccgctttc attagaacag gttctttttt cttcaataaa aaggcaataa
    42301 taaagccaag aatcccaaag atgatcgcta cgataaacgc ggcatggtaa cctgacagtg
    42361 tggcattgat tgcctgatct ttataagcta atggcgtatt atccaataca gatttgcttg
    42421 ggatatttgc ttttgtcaca ttcgtcaaca cactgatcaa aatagctgta ccaatcgagc
    42481 ttgcgatttg acggaatgta ttatttaccg ctgtcccatg actgatcaag ttatttggca
    42541 atgcattcat accagaagtc gttaccggca tcatgaccat ggagatccca aacatacgga
    42601 tcgcataaag gatcacgata tataaaactg gagtcgtttt tgtcaagaaa gcaaatggtg
    42661 cagttgccgc tgtcaaaatg aacatccctg tgatggctaa acgtttcgcg ccgtatttat
    42721 cgaaaatcgc acccgtcaca ggcatcatag cccccatgat caatgcaccc ggcaacaaca
    42781 tcaagccaga atggaatgct gattctccac gaatattttg aatatacaat ggtacgacca
    42841 tttctgcccc taccatcgcc atatttgtta caccagctaa aacagctgca atcgtgaatg
    42901 tagatgattt gaatacacga agttccaaga atggatgttc caaatgtaat tgacgccatg
    42961 agaacaaggc gatgaaaatc acaccaacaa ctaagaaacc aattactttc aagcttcccc
    43021 agccgtcatc tcctacacta gaaaatccgt aaagtaaact accgaatcca atcgttgata
    43081 aaatagcgga taaaatatca atgctaggat ttgacaatgg aataacactt ttcattagga
    43141 agaatgctaa aaccaagaca agaacaacga ttggcagtac cataccaaac aagtcacgcc
    43201 aatggtagtt atcaatgata taaccagata atgtagggcc taaagcagga gcaagtccaa
    43261 taacgatccc tgacatcccc attgctgatc ctcttttttc tggcgggaaa atagatagca
    43321 tgatattttg caataatggc atcgatactc ccacaccgca agcttggatc aaacgaccga
    43381 ttagtaatgt actaaaatta ggtgctgtaa agcaagtaat cgttccgatc aaaaagatcg
    43441 tcatggcaga aagatataaa tttcttgaac tgaatttatt gatcagccat gctgttattg
    43501 ggatcatgat cccgttgact agcaagaaac cagtcgttaa ccattgcaca tcagacgctg
    43561 aaatatcgaa agctttcatc aatgtaggaa aagctgtcgt caataaggtc tgattcaaaa
    43621 cggtacaaaa tgtaccaatt aataagacaa caactaaaag tgtccgatta tatggttttc
    43681 cgtaaatatc tacgggttgg ttgttactca tatttctcct tctttctgaa ttttttctga
    43741 aactactaga atactttagt cctaaattcg ctatttgtca acctacttga cacataagtt
    43801 aatacggata taattaaggg aagcaagagg acaaaaggag tgacaaaaat ggttggggct
    43861 tcccttaaac gtttgttgga aaatgatcaa ttggttgttg gcatcagtga attaagtgat
    43921 atcgtaggtg tgtctccacg tcaattaagg tattgggaac aaaagggatt cattcgttcg
    43981 atcgaagctg atgcgaattg tcctagaaaa tatcgtttac ctacagtcat aaaagtagaa
    44041 ctgatcaaaa aatatttaga tgacggattt actttgtcaa aagctgcaga aaaggcagaa
    44101 atccgccaaa agaaaatgca ccatgtccgt aaagtttttt caaaagcaat caaagatatc
    44161 gaattgatcg acgaacgcta tacgatttta caaatcggta acttcaaaga agagaatgct
    44221 aagatgtaca tcattcatga caacgagaca gacgaattaa cttacaagct ccttcctgtc
    44281 gatgaaaaag ctgactacga acagttgtta aaagaactct aaacgccact aatctcctaa
    44341 gtaccaagct ttttaataag tagatgctac ataaatactt gaaaaaaggc ctgtttgaac
    44401 ataagaatat ccgaacggtg atctgcgttt ctttcatata gaaaggaatt ctccatcact
    44461 gttcggatat ttttttattt ttttcggtta ctttcttcta atacatctgt tagccaattt
    44521 gcatattctg tttcccgatt gatcgcatgc gctaaaatca ggtagtgtcc gtaattttca
    44581 tgtttttctt tttcagtagg aaagatcgtt ttttttcgct ctaatagatg aagtcgtttt
    44641 tcttcatgaa ggtcacgctg ctgctgaata atttctacta atgccggatc attcacgtct
    44701 ttgacgaaat acaatttcag aacaaattcg tctcgattaa ccggtagttc attggtgggt
    44761 gtatgaatcc actctgccaa ttgttccttc ccttcttctg ttaattcata aactttcttt
    44821 tccaattttt cacctgtgat ctctacttga tgctgaatga ttccttgctc ttccattttt
    44881 gcaagttcag gataaatctg actatgtttt gcttgccaaa attctcctat ttcatttttg
    44941 aacgattgat tcaattcata tcctgtcatc ttcttcttac ttaataaccc taacaaaata
    45001 tatcgaagtg tattttttct tgccatcagt tttccctcct tgacaaatct ttaaatccat
    45061 tctatcataa atgagtaatc gaaaacatgt agtttattac atgttttaat ttataaggag
    45121 atgcttaaac atgaaagaat ttaaagaatt ggaagatttt attggaactc actttatcta
    45181 tacgtatgac aatggttggg aatatgaatg gtatgccaaa aatgaccata ctgttgatta
    45241 ccgtatccat ggcggaatgg tcaaaggccg ttgggtaaaa gaccaagaag ctgatatcgt
    45301 taaattgacg gacggcgtat tcaaaatcac ttggacagag ccaacaggga cagacgtggc
    45361 attggacttt atgccaaatg aaaagaaact tcacggaatg atctttttcc ctaaatgggt
    45421 tgaagaacat ccagaaatca ctgttacttt ccaaaacgaa catatcgcag aaatggaagc
    45481 agctcgtgaa aaatatgaaa cttatccaaa attacttgtt cctgaatttg ctacgatcac
    45541 ttatatgggt gatgcaggta tagacaatga agatgttatt agcgaagaac catacgatgg
    45601 aatgccagat gatatccgta gcggaaaata cttcgatgaa aattataaac gagtaaaata
    45661 aggttgtgaa agagaggttg tgaaatcagc gttctgacct gagtattaga gaaagaaacg
    45721 gaaaaatagt tcctcatgtt ttgccgtttt ttgagctaat accgagggtc agctgattga
    45781 acaccgttta tcaagaggtt gtgaaatcag cgttatgctt tgagtaataa gaacgctagt
    45841 tagaaaatag cttttcatat tttctaggct agtgggctta ttaccgagaa gcagccgttt
    45901 gaacaccgtt tatcaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    45961 atggaaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    46021 tgaacaccgt ttatcaaaga ttgtaacaac tgcatattca cacagttaaa ttagtgactt
    46081 caaaatgaaa taatgctccc agcaaaaaat taggctggga gcattatttc gttattctgt
    46141 tcatcaaaaa tcagcgtttt gattccattt catcacaagt tcctcctcat ttactgcttc
    46201 atctttttgc tgtgtttgaa ttgtaaaacc ttgtgaaaga tagaaatgaa tagctgattg
    46261 atttttttga tagactgtca aagtcagtgt tttattggtt tcttttattt ttttcattag
    46321 ttgcgtaccg attctttgtt gccggtattc ccttttcaca aagattcctg cgataaactg
    46381 atcaatcacc ccagcaaaac caataatttc ttgatctatt tctgctaagt aaatcactgc
    46441 atctggtaaa gctttcctaa cagattctac ttgattctcc caataagttg atggaatgaa
    46501 ttcatgagaa tccaagttag catgcagcca aatcgataga acagattcca gttctttggc
    46561 agacaatttt gttgttgtgc ggatcatttt ttctctcctt acttattcat tgattttaaa
    46621 ataaaaacca tttttttgaa gctcttttac aagttttcgc ccctcaaaaa agtatatctt
    46681 ctcttgtttt acttttctaa taccaaaaga aacagcaact ttaggatcat cagctgaaac
    46741 gattcgataa gttccaccag gcaattgatc cagcttgact ttcccatgat ggtcgctttg
    46801 tactgaaaac ggcacaccat ttctagtaag tgtttttttc ttatgtttca aaatgaatga
    46861 actaccattt accggtttcc ctgacgaatc actaataata aatgaaaaat caagcacttt
    46921 tttacttaat ctatactgat agattagtcg tccgataaac actaacagta tgactacagc
    46981 tattatcgtt cctgcaagaa taaaccattg ccgcatggtt ctttcctgat ttccttttcc
    47041 ttcttctttt tttaccgatt cgttataagg aatccgatgt cctgtcacta gcaaacgatg
    47101 cgtgttgatc atatacggcg tacatgtaag caaggtaact aagtcttctc cttctgaaat
    47161 cgttagaaca gacgtgtcat ctggcagtac cacttgtata tttttaactt gataagccaa
    47221 tttctccccc aaaacagtca aaataaacgt gtctccgttt tctaaacgat ctaattcggt
    47281 aaataaccgt cgattaggta aaccgctgtg agcagagatc actgaatgcg tcgaatttcc
    47341 accaagcggg aaagaggttc cttgcaagac gcctgctcca ttttcaagga tcctaccatt
    47401 gagccgatca taaagtgaaa tatttaattg gatcttaggt atattgatac ttccgatcag
    47461 ccattcctct aattggctgg atgttgtact ttcatttctg ttcttttcat tgaaaggatc
    47521 agtaccagga ttgaatcctt ggtcagttaa tcgttggttt ttctcctcca ttttctttcg
    47581 ctgcacttca atttcagctg cggtccgttt ttgtacttgt tcgatacgtt tttgatcaat
    47641 aaaactattt aaagaatcca cataaaatgg atataaagcg atcagtaacc ctacgacgat
    47701 acatattaat aaaaacaaac gaaacaaaag tcgacgagac tgtttttgct tactctgctt
    47761 cacatgtgtc ctcccttgca agtttttttg ttctttcttt tattatatgg gttcaggaaa
    47821 aaaatagaaa atgaaagccg atgaggttgt gaaaaagctg tcctgcatca agtaataagt
    47881 acagcccaaa aaaatagcta ctcatatttt ttgttgggtt gggcttatta ccgcagatgc
    47941 aagcttttga acaccgttta aggaagaggt tgtgaaaact gcgttatgct ttgagtaata
    48001 agagcgctag tcagaaaata gcttctcata ttttctaggc tagtgagctt attaccgaga
    48061 agcagctgat tgaacaccgt ttaccgagag gtcatgaaaa agctactttg tttcgagtat
    48121 taagaaaaat tctgtttcaa aacaaggaga tgcaacaaaa cttatgtcgc atcctctctc
    48181 cttagaactt tcacttatgt ttttcttgta aaattcataa atattttcat gaattttcta
    48241 atttcttgcc tacctctcgc ataggatata tgatgaattt atctaaagtt tgcaaaggag
    48301 ttcgacaatt gaaaaaatat ggcccatttt tagcaatttt tttcattgct ttggcatttc
    48361 tcatgcctca tttattcaca cataaactaa ttttgggtgc tgatgcccca ttccattaca
    48421 atcggttcta tgatgccgcg caacaaatta aatatgggga tttccattat tttccttccc
    48481 tttatggatt ccagcaaagt ggcaggatcg tgaatgccgt ttacggccca tttttcgctt
    48541 atttacaagg attgatcgtg ttgcttgcag gtagctggtt tcattatcag attctttcaa
    48601 atttcattct ttatcttatt tcaggatttt ctatgctgat tttactgaga tatgtcaatg
    48661 ttcgaaattg ggtaagtgta ccagtagccg ttttattcat gagtacgtat tctattcaat
    48721 actggtctat caaccaagga ttcactagct gggggacttg tttttatcct ttatgtatga
    48781 tccctattgc tgatttcgtc cttcataaaa agttccctgt tatcaaagtc gctgtctcag
    48841 tgggacttat gacacagatc catttactgt cttcgatcat gttagctttg atgtatatcc
    48901 ctttttatct ttattacttt tttaaccaga ctgaaaaagg aaaagcactg cttcaactga
    48961 ttaagtcggt cttgctttat tttgttctga cagcaaatat ttgggcagga ttagctattg
    49021 tttacagtgg gaataagatt cttcctccct ttgttaacag agaaatgagt caaaaggcga
    49081 tagatctgga tggcagttat tggctccatt accctaaggc ctttcctata ttgatattat
    49141 caggtttttt gctttttatt ttctttcaac aaaagaacac cttattccaa aaaatcgtgt
    49201 tgtacacaag tcttgttttc ttttttcttt ctactagctt gattccatgg acgacattga
    49261 tcgaaaaaaa tgtgcctttt attagtttga tccagtttcc atttcgattt ttcgtgccat
    49321 ttacgctatt gcttctactt taccttgccc tttcactgaa ccagtggtct gaacaaaagt
    49381 ggttccgtct tttaagtata tttggcattc ttattttttc agcacagacg atccagaact
    49441 tataccagca tctggaaaag tgggaaaatg aaacatttgt cagtcgacat acttatctat
    49501 tcgatacgcc ggaagaagca aggaaaacat tcttcagcaa agatatgtct accagcttgt
    49561 tcgttttcca aaaaaccaca cctgactatc tgccaatcta taaggagaca aaagacaata
    49621 aatacgaccg gtataacgaa gaaatactag aaaacgaaac aacttttata aaaacacatc
    49681 caggcggtct tttgacaatc aagtggaaaa acactgggga caaaactgtt catatccctg
    49741 ttatcgtcta cgatcggact gtgctacagc aaaatggaaa aacattgact gattatgaag
    49801 taacagatat cgggacccca atcgtgaagc agcaaaaagg aatcaacgag ctcacgcttc
    49861 attatcaaac gccaatctat ttctatttca ttctttctct tactttgatt ggctggttta
    49921 ccttgctatg tttatttatt tatcatagat acaagctttt acgtgcttag gcaaaaggga
    49981 taaatacatc cgatcaaaaa actctccaaa cggtataatc gtttggagag ttttatttta
    50041 atcagttttt ttatgttata tgttatattt tttattcgtt cacatctaat tcgatttcca
    50101 ccccgtagtg atcactgatg attggactat cgatcccatc aaaaatccgt tgatattttt
    50161 taacctgcat tccttcaggg acgaaaataa aatctatacg caatttttct gtattttgtt
    50221 cccaaccatc tattttcttt tccacagtag ctgcttttcc tttttcattc gcgacaacat
    50281 acgaatcttg aagaagaagg caactttctc ttacagtata ataaccttga gagtctactg
    50341 gattattgaa atcacctagg aaaaaaagtg gtacgcgagt ttccaaaaag tagttttcca
    50401 agtttttcca ctcaaaataa aagccacttt ttttatctgt ccaccaagaa aagtggcagc
    50461 tcgagacaaa aatgtcctga tcatctatct ttgttttccc aatcaagatc ttgcgtgtct
    50521 gataatcttc tttcttttga aattgggaga cggtaaggac ctgactttcc aatgtttttt
    50581 tcgtaagcag tgcatttcct tcttcatatt tatcataacc gatgtggctc atttcccaac
    50641 tccaaaaata ctcttttccg tgttccttta aaagctgaac taaacggtag gcaaaattat
    50701 ctttacggat aggtacttga ttctttaccg gacagaactt cattagcttc gcaggttcta
    50761 cttcttcaga ttccagtaat tggttgattt cttgtaaagc aatcacttca taatcttcta
    50821 acaggatctt atctgctaat tcttgcaatt ttttctctgg ttctggctca agccagctat
    50881 gcgtgttgag tgtcatgaat ttcattggtt ttctccttgt cttcatttcg aaacaaaagg
    50941 gttgtgacaa aatttttgtc acaacctctc cagcaaatag tgttctcctg ctaactcctc
    51001 agtaataagc tcaatggacc aggagctatt ttttgtttgg ctgtttttat cacttggtgc
    51061 agggcagctt tttcacgacc tctccactaa acagtattca aactgctgct tctcggtaat
    51121 aagcccaacc aaacgaaaaa taggaggagc tatttttgtt tggctgttct tatgacttga
    51181 tgcaggacag ctttttcaca acctctcctt acattttaat ggagccgatc actttaccag
    51241 ctgtcactgt acctaattgc ttgatcacta cttcttctac tttatcgttt gtaaatacga
    51301 tcatgatcgt tggatctttt tcttcgtttt ggatcacatc aagatccatc tcagctaatt
    51361 tcgttcctgt tcctacttct tggccttctt tgacagcaat ctcaaaagct ggctgattca
    51421 tttggactgt atcgattccc atatggatca aaacttcgat accatctttc gtttccagcc
    51481 caattgcatg tttgctaggg aaaatacttg tcacttttcc agcgatcggc gcaacaactt
    51541 ctcttgatgt tggaataaca gcaaagccgt cacccatcat tttttgtgag aataccggat
    51601 cttttacttc agataaagga atcacttctc cagaagctgc ggaaactagt tcttgttcta
    51661 tacctaagta ttgcttacta cctgcttgtc catttcctgt agaaaccatt tctggctctg
    51721 gaatctctgc tcctgattgc agcagatctt cgatatctga tttcaagaca tctgcttttg
    51781 gtccataaac tgcttggact ccattgtctt tgacgatcaa tcccatcgcc cctgctcgtt
    51841 tccaggcttc ttcagtccct actttttctc gatcatttac acttacgcgt aaccgagtca
    51901 tacaagcatc gacatctttg atattttctt ttcctcccag caaatgaatg atctggacca
    51961 cttgctgatc tgctgttcct gttccgctgt tagatacaac ggtttcttca ctgtcattgt
    52021 cgtaatttcc gttacgtccc ggtgtagcaa agttgaattt tttgatcaag aaatttgcta
    52081 agaaatacgt aacaacacca aaagcgatca cacacaacac gaagttgata agatctccac
    52141 ctagacccgc tttgatagct aacggtgtcc gagtcagcaa ttcgatattc ccaaatgaat
    52201 ggacccgcaa tggcaagata tctgccatcg caaatgcagc cccttggatc actgaataaa
    52261 tgacatataa tggcacagca gcaaacataa acatgaactc caatggttct gttacacctg
    52321 ttagaaagac agccaacgct gctgaaaaat acattgattt atatcttgct ttcttatcag
    52381 gatcgacatt acgatacatc gcaaaagcca ttcccatcaa gatacctgaa gaaccaatca
    52441 tttgtcctac tttgaaacga gccggcgtcc agttatgcaa cacaaactca tattgcgaca
    52501 tatcccctgc acctttaaga ttcactaaat cagtggccca agccaaccat aacggatctt
    52561 gcccaaatac ttgtgtcccc gcctgtgcgc ctgataaaat ctcatacgat ccgccaagct
    52621 gtgtatagtt gataggaatc gtcagcatat gatgcaatcc aaatggcaat agtaaacgtt
    52681 ctaatgttcc aaataaaaac ggtgctagaa tcggtgcgct ttcttgtgat tctgcaatcc
    52741 ataaaccaaa attattgatt ccagcctgta cgtttggcca caccacagca agaatcaatg
    52801 ccacgattgt tgaccataag ataacaacaa acggaacaaa gcgctttcca ttgaaaaatg
    52861 acaaagcatc cggcaatttt ctataattat agtacttatt ataagccatt gccccgacaa
    52921 atccagcgat gatccctacg aatactccca tattcaaagc tggtgcttcc aatacacttg
    52981 taaaaaatcc tttaaccatg atcttcgtac cgaacagagt gtgggtaaat gcatcttcat
    53041 tcgcaagcat gtcacttgtg acaccaaaga tagccccagt gatccgattg attaaaataa
    53101 atgagatccc agctgcaaat gctccgcctg ctctttcttt tgcccaactt cctcctattg
    53161 ctaaagcaaa taaaatatga agattaccaa taattgccca accaatattt tcaattactc
    53221 cgcccgttgt gacaagcggc gtccaatcag gattgatcaa gggaattgat ttccctatac
    53281 tgatcatcaa cccagctgcc ggcataactg ctaccacaac cattaaagcc tttccaaatt
    53341 tttgccaaaa ctcaaaacta aatatctttt tcattccgtt tagctccttt acgcaatcgg
    53401 tttcgttaaa cgtattatag cgtgcgtttt cttttttgta aatgctttat tttttatttt
    53461 tctgtctata ccattctata aaacctataa tacgtgtgtt cccttctata attttctcct
    53521 aaaaatttat ttttttaagc ttttttctat gaaatatttt cttttgtcta ttgacttgtg
    53581 caatcggttg cgtttattat gaacgtataa aacagctcat taggaggaga aaaatgaaac
    53641 agatcaaacg actatttgct attgatccat ggaaaatcag cacgacacaa ttagataaaa
    53701 aaaatcttcg actgcaagaa tctttaacaa gtattggaaa cggttatatg gggatgcgcg
    53761 ggaactttga agaaacttac tcaggggacc accatcaagg tacttatttg gcgggtgtct
    53821 ggtatcccga taagacacgt gtcggctggt ggaaaaatgg ttatccagaa tactttggaa
    53881 aagtgatcaa cgccttgaac tttatcgcga tagatatttc gatcaacgat cagctgatcg
    53941 acttggcttc tttggaacca gaagactttt actgggaatt agatatgaaa aatggtgtat
    54001 tatctcgtac attcacaatc actacttcta ctaataaagt acgcatttcc ttcgaacgtt
    54061 ttctgagcat tgtcaaaaaa gaatcagctt atatctattt aactatagaa atgcttgaag
    54121 gtagcggaac agtcaaactc gtatcaaaat tggatggaaa tgttcaaaac gaagacagca
    54181 actatgatga acatttttgg aatgagattg atcgtggaac tatagaagaa atcggctatt
    54241 tgacgactaa aactattcca aacaactttg ggattgaaca atttactgtt actgcagcaa
    54301 tgcgccattt cgttaatgga aaatcagtca tccctgacta tcacgaagat actttagcat
    54361 tacatggttg cttgtctgca gaattatctc aaggtgagac atggtcagtg gaaaaagaaa
    54421 ttatcgttct tactagtaga gatattccag aagcgcagca agcagaaaaa gctgtctcag
    54481 aattaacgaa ccagacaaac aattatatcc aagcaaaaga agagcaaaca gcggcttggg
    54541 cagaacgatg gaagcttgcc gatgtggtca tcgaaggaga cgaagaagca caacaaggta
    54601 ttcgattcaa tttgttccaa ctattctcta cttactacgg cgaagatgaa cgattgaata
    54661 tcggccccaa aggattcaca ggagaaaaat acggcggagc cacttactgg gatacagaag
    54721 cgtatgctgt ccctctttat cttgctttag ccaaacctga agtaactaag aatctgctga
    54781 aatatcggca taatcaactg ccgcaagcaa tccacaacgc tcagcaacaa ggattaaaag
    54841 gcgcgcttta tcctatggtc accttcactg gtgtcgaatg ccataacgag tgggaaatca
    54901 cattcgagga gattcaccga aatggcgcga ttgcctatgc aatctacaat tatacgaatt
    54961 acaccggcga tacggaatat ttgaaaaatg aaggactgga agttttagca gaaatcgcac
    55021 gcttctgggc agaccgtgtc catttttcaa aacgagcaaa taaatacatg atccacggtg
    55081 tgaccggccc aaacgaatat gagaacaata tcaataacaa ttggtataca aatacgatcg
    55141 cagcttgggt cttgcgttac acgcgagaaa attatttaac ttatcaaaaa gaagctgcta
    55201 tcactatcag cgaagaagaa ctagccaaat ggaaagatat cgcagagaac atgtacttcc
    55261 cagttgacga agaattagga atctttgtcc agcatgacac attcttagat aaagatctca
    55321 tgcctgtatc cgatattccc acaagcgatc ttcctctgaa tcagcattgg tcttgggaca
    55381 aaatccttag atcttgcttc atcaagcagg cagatgtact acaggggatt tatttcttca
    55441 atgatacttt tacaaaagaa gaaaagcaaa aaaactttga tttctatgaa ccaatgactg
    55501 tccatgagtc ttccctttcg ccaagtatcc attcgatttt agcagcagaa ttaggaatgg
    55561 aaagcaaagc tgtagaaatg taccagcgaa cagctcgctt ggatttggat aattacaata
    55621 acgacacaga agacggcttg cacatcactt ccatgactgg tagctggttg tcgattgtcc
    55681 aaggatttgc tcaaatgaaa actgcccatg atcagttgag attcgcacca tttctgccaa
    55741 aaacttggaa agcttattct ttccatatca attatcgcgg acgtttattg ttcatctcag
    55801 tcacagatga acaagttact ttcgagttat tagaaggttc tcctctagaa atttacgtct
    55861 atgaacagtc ttattcttta gcggatcgtc tagaaataac gctaagaaag gaggaagcaa
    55921 atgtttaaag gggtattgtt tgatttagac ggtgtcatta ccgatactgc tgaattccat
    55981 tatcgtgctt ggcgaaagct tggtcaggaa atcggcatta cgatcgacca cacattcaac
    56041 gaacagctaa aaggcgtcag ccgagaagac tcactctctt tgcttttagc ttatggcgga
    56101 aaagaacact ctttttcaaa ggaagaattt gcagaattag cgaaaagaaa aaacgattat
    56161 tatttagaga tgatccagac gatcgaacca aaagatgtct tccccggggt cgtgccttta
    56221 ctggattctt taaaagaaga aaaaatcaag attgctcttg cttctgcaag taaaaacggg
    56281 ccattcctac ttgaaaaaat gggtcttaca ccttactttg atgctattgc cgacccggca
    56341 gaagcaacaa acggaaaacc tgcgccagat atcttcctcc ttgcagcaaa agctgtcgga
    56401 ctaaaagcag aagaatgtat cgggatcgaa gacgcgcaag ccggtatcca agccatcctc
    56461 tcaagtggcg ctcaaccaat tggcgtggga cgtgctgaag accttggaga ggagatcccg
    56521 cttgtccctg atacgacttt tcttacgatc gattacttga aagaaaagtg gcatgaccat
    56581 ggataatcca gagatctccg tcgtaccagt tccagagact gaaataaaga aaatcacatt
    56641 gaagaacgac tacttagaag tcgttcttct caattacggt gcccgcctgc accaaatctt
    56701 cgcaccagac aaagaaggaa aatcagaaaa tgttttattg tcttacgatc gtttcgaaga
    56761 tgttctaaca gacaaaagct tttttggagc aactgtcgga cctgtagccg gaaggatccg
    56821 ggatgcttct tggggtgagc atagattgga gaaaaactgc ggatcacacc atatccacgg
    56881 tggcacgaaa ggctggtctt tccaattttg ggaagtagaa gttttcaaaa ctccatcatc
    56941 aataggtgtg gtcttttact taaaagatga attttctacc tatccaggac caatcactgc
    57001 tacgatcact taccgtttaa caaaaaacga attagagatg acaacctctg ctagtagcct
    57061 tgttgaaacc atctgcaatc cgaccaacca tgcctacttc aacctttcag gaaatggaac
    57121 aagagacatc tatgaacatc agttaacggt ctttttagat ggtgtgcttg aattagatca
    57181 agaaaaactg cctactggaa attggacaaa aaaagaaaaa ctgccaatcg actttagaaa
    57241 atccccaaca ctgcaagaga tccttgcatg ttatccagat ggactagatg atgtatttct
    57301 tttgcatcat cctaggcttt ccaaaacttc cttacaatta ttcgagaagc attctggtcg
    57361 ccaaatgact atagcaactt ctaataaaag tatggtatta ttttctacga ctggattcga
    57421 agctgatttc tctgtcaatg gaaaacagat gcattcaaac tacggattag caatcgaacc
    57481 gcaagaaatc cctgatatcg ttcatttccc caaatttgga tcgatcaacc tccatcctgg
    57541 tcaagaacgg atcagtcaga cgatttatcg gttctctgca caataacaag agaaaggtgt
    57601 ttttatcatg gctattaccg taaaagatgt ggcgaaaaaa gccggtgttg ccacctcgac
    57661 cgtctctcgt gtcatcaacg atcatcctag tatttccgag acaacaaaga agaaagtccg
    57721 aaaaattatg gatgaactag gttatgttcc taacatgact gcccgcaatc taggaaaacg
    57781 catttccagt gccatcggcg taatcttacc ccctcttgat tcaaaagagc ggatcggtaa
    57841 cccattttat cttgagatca tggaagcaat caacgaagaa gcgcggaatt atgatatgac
    57901 tacagcaatc gctactgcta aaaattttga attgctgtta gagaacgtcc agcgtatgca
    57961 tttacaaaaa caagtagatg gttttattct agtgtattca gatagtcaag atactgtcat
    58021 tgattattta taccaaaacc acatcccttt cactttgatc ggacagcctt atcatcatga
    58081 gaacgaaatc gtttatgtag ataatgataa ccagcttcta ggtaaacaag caacagaatt
    58141 tttgatccag aatggacata agaatatcct gtttgtgaca aacacgactc atgaaagttt
    58201 gtatttcgaa cgatatttcg gttatcaaaa agcaatgatg atggctggtc ttcctgcgtt
    58261 atccagtgta gtactagagc atccggagga ttatctgtcc tttgagaaaa cgttgaaaga
    58321 aaacgaagcc acagcattgg tggtcattga cgatatcttc gcccttcgca cgatgcagtt
    58381 ggcgcaaatg tatggctatg tcattccaga gacactctcc gtcattagct tcaacaactc
    58441 gatcttctca acattaacac acccttacct tacaagtatc gatatcgata tctccgaatt
    58501 aggacgtgtc gctatgcaaa agctgcgcga attgatccat gaagaattga gcagtggcgt
    58561 acgtttggtc atccctcatc gcttgatcaa aagagagaca gtcgttcctt tagaaaaccc
    58621 atactaaata gctgactcac taaatacgct gaccatttat cctatggtca gctttttatg
    58681 taaaagaaga tactacttct aacacattgt ttatctgtaa aaaagagcgt gggacaaaag
    58741 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc
    58801 cttcggaaat aagctgaaat tcacaaaaat ttaaaaagca attttcttga gtttcttctt
    58861 atttcttgga gttaaacgct tctgtcccag cctctttttt ccttccttca attttttaca
    58921 cgtcgacgat tcaataaaaa accgcctaat attgtaaaga caggtgcaag aatcgagaaa
    58981 aaggcgaatg gcaagtaatg caacgcgcta actttcatcg ttcccataat gaatgtgccg
    59041 ctgactcccc acggtacgag agaattgacg gtcgcacctg aatccgctag tgttcgtgtc
    59101 aaataggttt ttgataagcc aacgtcatca aaagaacgct tgaatgtttc tcctggaaga
    59161 atgatggaca gatactgttc tccaacgatc agattgattc cgatacagct caaggcagta
    59221 aacccgacta aacgagaagg tcggtccatc ttttctttta attcttgaac gatggtatca
    59281 acgatcagat atttgatcag taacccacct aatgccaatg caagtaaaat caaactagca
    59341 gaacctaaca tactcatgat cccgcctcga gaaagcagtt catctagctt tggatcaggt
    59401 gtatttgcga cataaccatt catcaagatg tctgctgctt ttccgaccgt caaacttttc
    59461 tcatggataa aagaaagaac gagagcagct gtcgatcccg ctagtaatgt agggatagcc
    59521 ggaatttttt tgattgcccc cccaaataaa ataactagtg gaatcaatgt ccaaggagag
    59581 atccaaaagc tcgctttcat gttttggatc atttcattga tactagctag atcggctgaa
    59641 tgactgttat gtcctaatat cgtataaaaa atcaacgaaa ggacgaatgc tgggaaatcc
    59701 gtataacgca taccagcgat atgttcgaat agattaactc ctccaatacc cgctgccaag
    59761 ttggtcgtat ctgataaagg cgagcagtta ttccctaaaa aggcaccgga aacgactgcg
    59821 cctgtagtca ttgcatcatt gaatccaagt aaatgaccga tccccaaaaa agcaattcca
    59881 atcgtcgaaa tcgttgtaaa tgaactgccg acagtcactc cgacaattgc acaaacaaga
    59941 aatgctgtcg gtaaaaagaa acgcacagaa acaagtttaa atccataaac cataatcgta
    60001 gggatggttc cagataaaat ccatgaagca actaagacac caatcaaaag aaaaatcagg
    60061 attggaatga tccctggagt gattcctttg atgatcccgt catggatctc atcccatgtg
    60121 aactttttaa atctgccata aaacaacaaa cacatgaaag ctgcaaggat cgggatatgt
    60181 ggcgagagct ggaatccgat gatcagtact cctaggagtg ccaacaacac gatcaataca
    60241 gcgatacttt cttttaaaga aatattttta ttcatcgtcg atctcctatt tcaaatgaaa
    60301 aggccctgcc atgaaaaaac tattcacttg gcaggacctc ctactaaatc agcgacaaaa
    60361 cgtcggctga aagacttatt tttttatttt tcttctaaac ttttttctac ctcaatcccc
    60421 caagaatctt tcttgaagtg attgatgatc aatggaataa cgaacatgac cactgtgata
    60481 gctgctacta ataatgcatc actcaatgtc gctgtaccta atgtagcaaa tacgacaacg
    60541 atgatagaga agatcagcat gcagctgact acccaagcta atccgtttcc gtttttacca
    60601 attcggtaag gacgttcgaa ttctggatgt ttcttacgca ttttaagcaa tgaaacagca
    60661 atcaaaatgt acacgataca ataaataaca gttgtagcat ttgtaagtgt caagaataca
    60721 gcacttacgt catccattac accataaagc aaagcaaata atgaaataac gattgattgt
    60781 gttagaacga catttctaga tacgccgtat ttgttttctt tatggaatcc gaattttggt
    60841 ggaaggaatc cttcacgagc tacttggatg attgtttttg atggtccagt aacccaagct
    60901 gataattgta aaagcacacc aataaatacc atgaagctga agatatttcc gatcacttct
    60961 ggtaagccta ggatttgaca gtcaatcaag ataggctgcg tgatatttgc taattccatt
    61021 tttccatctg gtacgacatt tgctacaagc atcgcattga tgacattcaa taaaactaat
    61081 ccgatcaaag cgataaatac gccttttgta taattttttg ttgcgtcttt caaacgaggc
    61141 atgtaaacag aactcatttc gatcccaaca aaaataaatg tgataccagc taagtatttc
    61201 aatgaatcaa tatgttccaa gtcagggaaa gcttttgacc atgaaaagtc acctagatag
    61261 ccgtttgaga ctagtccgac tttgaaggct gccaaaacac ctaaaacgaa catgatgact
    61321 actgggatat atacccctag ccatacaccg atgttcccgc cgacttttgc catatcgaat
    61381 ttcaagttca agatcgtgat gatccagtag ataactaaaa tacaacctaa aacgaaccag
    61441 tgattattgc caagttctac attaccaaat gtgtttccta acaatggtcc taaagtagaa
    61501 gcgaccatta ccatacctgg gaacatttgc acccataata gccatgcaac gacaaatccc
    61561 catttgctgc ccagtgctgt ttttacccat aactgaggac cgccttcttg aggaagcatc
    61621 gtggaaagct caccggaaac cattgatatc ggaccggcaa aaaacaaaac tgcaaaaatt
    61681 gaataaaaaa tcaatgtcca accgactgcg gctaacgtag gaatactccg aaccgttgca
    61741 cacagtgcca ttgtcagacc gataaaggtc ccaagaccca ttttacttac ttcagattta
    61801 ctcaccgttg acactctcct tgattttttg gataaaataa cggtgtcttt gaagtagttt
    61861 cagcaataaa cgaaacaatt cagcgaatat ttgaggggat tctttgagtt tattgcttga
    61921 agggtttctt caaagacaac ctcaaaacaa agggatcgaa gagcttaggt ctccccttgc
    61981 tcttcagatc caaacatgcg tcagaaacag ttagtattca agctgttttc taacatgatt
    62041 accttgctta ttttgcttcg cttgccaata attggtctgc gtagattttt tccaattttt
    62101 cttgaagagc tgctttgatt ttttcagcat attcttcaaa gtgttcttct ttgttcatgt
    62161 atggtgtcat tacgctggca cgcaatacag ttacttttcc ggcacggttc cattcttcat
    62221 ctgagaatcc tagttggttc acaaattgta atgggctgtt gccatagtct gggatagcaa
    62281 agtcagtatg tgaagttaag aactcattac cgtaaatgct tcctttcacg tatgaagaat
    62341 aatcatatac gtcatggttc aatttattca ttgcaactaa gtcatcgttg cctttttctt
    62401 tgaatacata gtctaccata ttgaaatctg gataagttaa tggatgaact tcgatttctt
    62461 tgtctcctac tttaaatgac aagtcattca agaagttgta gaaacgatgt gcaccttcga
    62521 ttgatgcacc cattaattta ccatatcctg tgacattcaa aggtaagaca tggtgtgctg
    62581 cccaaacgct ggctgctgtt gcccctgctt ttgaaccttc caagatataa gccccaagta
    62641 atgctgggat atctgcgcct ttttcaaata catatgtcgc aaagtaagag ataacgtctc
    62701 tcatgcggat atctttgatg acgataccac cggcagaata tggaacatag cccattttat
    62761 gtgggtcaat cgttacagat tctgcttctt cgattgcttt atatgcactg tgtacttctt
    62821 ctaagatata atctttgttt tctgtaaaga cattgtattt gtaatgaaca tcttttaaat
    62881 cttcaaatgg aatgaagttg ttgtcttcgt ctaagaaaat cgcacggccg tatccgccat
    62941 aagcagcatc tacgtgtaag tagaagtaaa taccgtcttt ttctaatacg cgacgcaatg
    63001 caacgatttt atcgataccg tcgattgctc cttcttctgt tgatcctaca acaccgacta
    63061 cacctaggat tggtgttttt tcagctgcta gaccacgaac gattttttca agttcgttga
    63121 tgtccatgcg atagttgtgg tcaactggca ctggaatcac ttggtctaaa ccgataccga
    63181 tgatgtcggc agcttttagc catgaatagt gtttagtttg tggaaccaac cattttccta
    63241 atttttctaa atgttttccg ctacgtgctg agtgagcttt gatttcgtcg attttttctg
    63301 ggacgctgtc taacagattc atgatttctt ctgttgacaa gttcattaat tcccaatcgc
    63361 tttttccagc aactagttca ggtgtcactt ctttcattgc aagcggcaat gatttgatgt
    63421 tgcgggcata ccaaagtcct tctaagtttg ctaaagaacc atcagcaacg atgtggcccc
    63481 agccatcttt atagctcatt aattttgcaa attccattcc tacttcttct tccatttggc
    63541 ttgttgcagg agaagattca taggcaacgt tattaccatt ccatagcata gcaaaattgt
    63601 acgctagtaa agatggcatc aatgtttcag agttcatatg accccagtaa cgtccggcat
    63661 tatgccaagg aacggaatgg gtacgcatcc gcgctgaaat ttctgataga acatcctttg
    63721 ttttattcac agtgtgttca aaactagcac tgcttttttc ttctggcgta ataataggca
    63781 tgtcttgagg catgtagttt tgacgccagc ctaaatgttc atccaccaat tcattcaaca
    63841 aagctttata gatttggccg ttctcagctt tgtccccaat gaatagggca tttaggttta
    63901 gatctttcga caatgattca ctcatagtga aaccctccta aaaaaatatt taaattttgt
    63961 cgagccctac attacacctg ttcttctatt ctgtaaacta agttttggcc tgatttttaa
    64021 tcatatttta atatgtatca attatccgaa ttgttcacac ttttgaaata attaaaaaaa
    64081 acgagggaat ttacaaatgt aaactccctc gcaaagattg tattaacgac aaccagcgtt
    64141 ctattttagg atgggtttca aaattggaat gttgtaatag tatttccctt tttcaaggta
    64201 aaaacttact aaattctaat caattacttt ggccaaaaag taatttttct taccttttcg
    64261 aataaccaca aacttaccat cgaattcatc ggatggattt atgacaaaat ttaaatcggt
    64321 aacgcggtca ccgttaatac ttatagctcc gtttgaaaca tcttcacgtg cttgacgttt
    64381 agatggttca atttttgtgg aaacaagcag ttccacgata ttttcaggtg tgcttgaaat
    64441 ttcaacgttc ggcattttac cgaatccttg cgcgatttct tccgcattta gttctttaat
    64501 atttccagag aacaatgctt gcgttatttt ttgcgcttct ttcaagtctt cttcactatg
    64561 cacaaatctt gtaacttctt ctgccaaacg gcgttgtgct tcacgtttct caggttctgt
    64621 ctctactttt ttcgctaagt cttcgatttc ttcttgagac aagaaagtaa agaatttcaa
    64681 gtatttcact acatcgcgat catcttgatt caaccagaat tggtagaact caaatggtga
    64741 agttttcttc ggatcaagcc aaacggcacc gcctgcagtt tttccgaatt tcgttccgtc
    64801 agccttcagc atcaatggga tcgttaaacc gaacgcttct gcttctggtc cttctaattt
    64861 tcggattagg tctaatccag ctgtgatatt tccccattgg tctgctccac cgatttgcaa
    64921 ttgcacatcg tatgttttat gcagatgcaa aaagtcgata gattgcagga tttggtaagt
    64981 aaactctgta aatgagattc ctacttccaa acggctggca acgatatctt ttgctaacat
    65041 cgtgttgatg ttgaagtttt taccgtagtc tctcaaaaat tcaagtaaag agatttttga
    65101 caaccagtcg tagttgttca caaatgtgat atttgcatct ttaccaaaca attttctcat
    65161 ttgattggaa agtgcgtcca cgttatgttg cacttgttcc atcgtttgca acacacgttc
    65221 tgttttgcgg ccgcttgggt cgccgatcgt tcctgttccg ccgccaatca gaatatatgg
    65281 atgatggcct gccaattgga atcttttcat catcataaat ggaatcaaat gtccgatatg
    65341 catcgaatca ccagttggat caacgccgca ataaagtgaa atgctctttt cttcaacgag
    65401 ctcgcgaagt ctttcttcgt ttgtttgctg attaatggcg tcgcgccaag ttaattcatc
    65461 aataatattc attttactga cctccgtgta gtttttttat gaaagcagga acctgctagc
    65521 atatgtaatt aattatttct tcaggatagg ataggaaata aaaaaagtcc cttggcatat
    65581 aagccaaggg acgaaatctc gcggtaccac ccaatttgta tgtctagata tccaacatac
    65641 ctctcgaaca tttaacgctg tcgcgtggca atcactgcca aagactcctg aaatgtaatt
    65701 cgctttaaag catgtactgg tttgcagcga ccaccagctt tctgaaacat tggctttaac
    65761 aactacttcg tttcgatctt cgtcttgtta ttattaatat gtgtctatca tagagcgcaa
    65821 ctttttcttt gtcaacagaa aaaggatatt ttttttaaat tcctcatttt ttcttttttg
    65881 aatccgctct aattccgaac attttatttt caattattgt taaaagaaat aactttttct
    65941 gtattttttc tgcaatttat cgagaagtca ctttactttg cctcttattc tttcttcgtt
    66001 acaataagga atgggacaag taaaaaaatg aaaaagcaag ggacgaggta tatggaaaaa
    66061 aagcaaaaaa ttattttagg agccacttct gctttaacta tcgttatttt aattctaagt
    66121 ttgttttttg tcatccgatc atctacaaaa aaagaaccaa tcattcctga aaaaaaaata
    66181 acagaaaaag caaaagaacc gacagatgat aagcaacaac tagttactca agcaaaaaat
    66241 ctggctcaaa gctacgatta tgaccaagcg attgcgttat tggaaaaaga tagtaagaac
    66301 ggatctactg aaacacaaca gcttcttcaa acactaaaaa aggagaaaga acaactgatc
    66361 gactggtctg atcctactca gatctctcat gtctttttcc acagcttgat cgtggacccg
    66421 caaaaagcct tccactctca acaggcacaa ggatacaaag attatatggt gactgttgaa
    66481 gaattcaacc gttcgatcga acaactttat caaaatgatt ttgttttagt taatttgaat
    66541 gatttgatcc aaaaagacga acaaggaaat ctttccttta acgggcttgc gctacctgaa
    66601 ggaaaaaaac cattggttct ttcacaagat gatgtcagct attatgaata tatggataat
    66661 tccggttttc ctagccgatt ggtcgtatca aataacaatg aaatcaaaaa tgtctataaa
    66721 gagaaagata aagaagaaat cggtgacttt gacatggttc ctctgatcga tgcctttgtc
    66781 aagaaacatc ctgatttttc ttatcgaggc gcaaaaggta ccttggccct gacaggttac
    66841 aacggagttt taggttatcg aacatcaaaa tctcaatatg gagacaatga aaaaacaaaa
    66901 aaagagatta aagaagctaa aaaagtggcg gaacagttga agaaagacgg ctggagcttt
    66961 gcttcccata catggggtca cttgaatatg actcaagctt cactagaaga tgtaaaaaaa
    67021 gacaatgaat tgtggcagca agaagttgca cctatcctag gaaaaacaaa tattctgatt
    67081 tatccatttg gtgcagacat cagcgactgg cagccttatt cagcagaaaa tcaaaaattt
    67141 gcttacttga agagtcaagg tttcgatatt ttctgtaatg tcgatgcttc tacaccagct
    67201 tggggacaat taggtacgga tttctatcga aatgctcgga tcaatatcga tggcattcgt
    67261 tttcaatctg atttagacgg aaaaaatcct attcttgatc cattcatcaa tgtccatgat
    67321 gtctatgaca aagaagcacg cgaatctgct tgattttacg tcatttatga accaaaaaag
    67381 ccgaactgac attttatcgt cagtttcggc ttttttatgc tttatttctt cttcggtgcg
    67441 attcccttga tcaggacttc tgtccattct gctgtatatg gaagcagttt tgcgtatatc
    67501 tccggcttag aaattccttc taccattgct tgaaaataga ggatcaagaa ctcatccgaa
    67561 tatttcaaat ccacttttcc ttctttccgc cctcgatgga aaagatcaag catgatactg
    67621 aaactttctt gcgtatacgc atccatcgcg tcagaaaatt cttttttttc tttttttgta
    67681 tagtatgtca tcaaatcgat ataaaattgt ttgttcactc gattgagata acggattttg
    67741 ttttcactca tggcaatcag tgtttcttca aatggaagat tttccgcaat caacgctttc
    67801 gcttcttgcc ctaaatcatt cataaatgca aaaaatactt cataaatcag cccttccttg
    67861 ctgccaaaat atttaaaaat cgtcgcttta cttacctgag cgttctcagc taccttatcc
    67921 attgtcactc cagcggttcc tatgtctgtg ttcatcaagt caaaagctgc ttgtaaaatc
    67981 tctttctttt tctcttgtgc tctcttttca aaaccgttca tttgtagagc ctcccgtaat
    68041 tttaaacttt tataatatat atagttcaaa tttattctga ggtttattat aactatctat
    68101 tgagaaagta tcaagctagc aattccttgt ttatg
//
LOCUS       DAZKMB010000003        60238 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000003 DAZKMB010000000
VERSION     DAZKMB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.003"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..288
                     /locus_tag="ACG8V3_000129"
     CDS             <1..288
                     /locus_tag="ACG8V3_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298704.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="ACG8V3_000130"
     CDS             347..787
                     /locus_tag="ACG8V3_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298705.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="ACG8V3_000131"
     CDS             799..1467
                     /locus_tag="ACG8V3_000131"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0298706.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="ACG8V3_000132"
     CDS             1531..2154
                     /locus_tag="ACG8V3_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0298707.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="ACG8V3_000133"
     CDS             2210..2596
                     /locus_tag="ACG8V3_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0298708.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="ACG8V3_000134"
     CDS             2809..4200
                     /locus_tag="ACG8V3_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0298709.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4811..5179
                     /locus_tag="ACG8V3_000135"
     CDS             4811..5179
                     /locus_tag="ACG8V3_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0298710.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5359..6300
                     /locus_tag="ACG8V3_000136"
     CDS             5359..6300
                     /locus_tag="ACG8V3_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0298711.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6369..7100
                     /locus_tag="ACG8V3_000137"
     CDS             6369..7100
                     /locus_tag="ACG8V3_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0298712.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7244..9424
                     /locus_tag="ACG8V3_000138"
     CDS             7244..9424
                     /locus_tag="ACG8V3_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0298713.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9444..9926
                     /locus_tag="ACG8V3_000139"
     CDS             9444..9926
                     /locus_tag="ACG8V3_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0298714.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9942..10703
                     /locus_tag="ACG8V3_000140"
     CDS             9942..10703
                     /locus_tag="ACG8V3_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0298715.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10834..12168
                     /gene="murC"
                     /locus_tag="ACG8V3_000141"
     CDS             10834..12168
                     /gene="murC"
                     /locus_tag="ACG8V3_000141"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0298716.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12475..12960
                     /locus_tag="ACG8V3_000142"
     CDS             12475..12960
                     /locus_tag="ACG8V3_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0298717.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12953..13636
                     /locus_tag="ACG8V3_000143"
     CDS             12953..13636
                     /locus_tag="ACG8V3_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0298718.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13762..16407
                     /gene="polA"
                     /locus_tag="ACG8V3_000144"
     CDS             13762..16407
                     /gene="polA"
                     /locus_tag="ACG8V3_000144"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0298719.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16452..17288
                     /gene="mutM"
                     /locus_tag="ACG8V3_000145"
     CDS             16452..17288
                     /gene="mutM"
                     /locus_tag="ACG8V3_000145"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0298720.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17252..17881
                     /gene="coaE"
                     /locus_tag="ACG8V3_000146"
     CDS             17252..17881
                     /gene="coaE"
                     /locus_tag="ACG8V3_000146"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0298721.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17933..18265
                     /locus_tag="ACG8V3_000147"
     CDS             17933..18265
                     /locus_tag="ACG8V3_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0298722.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18577..19068
                     /gene="nrdR"
                     /locus_tag="ACG8V3_000148"
     CDS             18577..19068
                     /gene="nrdR"
                     /locus_tag="ACG8V3_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0298723.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19091..20464
                     /locus_tag="ACG8V3_000149"
     CDS             19091..20464
                     /locus_tag="ACG8V3_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0298724.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20464..21390
                     /gene="dnaI"
                     /locus_tag="ACG8V3_000150"
     CDS             20464..21390
                     /gene="dnaI"
                     /locus_tag="ACG8V3_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0298725.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21519..21959
                     /locus_tag="ACG8V3_000151"
     CDS             21519..21959
                     /locus_tag="ACG8V3_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0298726.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22103..22804
                     /locus_tag="ACG8V3_000152"
     CDS             22103..22804
                     /locus_tag="ACG8V3_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0298727.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            22801..23922
                     /locus_tag="ACG8V3_000153"
     CDS             22801..23922
                     /locus_tag="ACG8V3_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0298728.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            23937..25169
                     /gene="pepT"
                     /locus_tag="ACG8V3_000154"
     CDS             23937..25169
                     /gene="pepT"
                     /locus_tag="ACG8V3_000154"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0298729.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25301..26209
                     /locus_tag="ACG8V3_000155"
     CDS             25301..26209
                     /locus_tag="ACG8V3_000155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0298730.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26244..26516
                     /locus_tag="ACG8V3_000156"
     CDS             26244..26516
                     /locus_tag="ACG8V3_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0298731.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26527..27573
                     /locus_tag="ACG8V3_000157"
     CDS             26527..27573
                     /locus_tag="ACG8V3_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0298732.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            27824..30433
                     /gene="clpB"
                     /locus_tag="ACG8V3_000158"
     CDS             27824..30433
                     /gene="clpB"
                     /locus_tag="ACG8V3_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0298733.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30584..31270)
                     /locus_tag="ACG8V3_000159"
     CDS             complement(30584..31270)
                     /locus_tag="ACG8V3_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298734.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31248..31742)
                     /locus_tag="ACG8V3_000160"
     CDS             complement(31248..31742)
                     /locus_tag="ACG8V3_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0298735.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(31762..32982)
                     /locus_tag="ACG8V3_000161"
     CDS             complement(31762..32982)
                     /locus_tag="ACG8V3_000161"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0298736.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33129..34964
                     /gene="lepA"
                     /locus_tag="ACG8V3_000162"
     CDS             33129..34964
                     /gene="lepA"
                     /locus_tag="ACG8V3_000162"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0298737.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35160..36071
                     /locus_tag="ACG8V3_000163"
     CDS             35160..36071
                     /locus_tag="ACG8V3_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0298738.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(37011..37970)
                     /locus_tag="ACG8V3_000164"
     CDS             complement(37011..37970)
                     /locus_tag="ACG8V3_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0298739.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            38148..38384
                     /locus_tag="ACG8V3_000165"
     CDS             38148..38384
                     /locus_tag="ACG8V3_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298740.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            38497..38739
                     /locus_tag="ACG8V3_000166"
                     /pseudo
     CDS             38497..38739
                     /locus_tag="ACG8V3_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            38829..39068
                     /locus_tag="ACG8V3_000167"
     CDS             38829..39068
                     /locus_tag="ACG8V3_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298741.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            39215..43498
                     /locus_tag="ACG8V3_000168"
     CDS             39215..43498
                     /locus_tag="ACG8V3_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0298742.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            43597..43962
                     /locus_tag="ACG8V3_000169"
     CDS             43597..43962
                     /locus_tag="ACG8V3_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298743.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            43964..44416
                     /locus_tag="ACG8V3_000170"
     CDS             43964..44416
                     /locus_tag="ACG8V3_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298744.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            44865..46340
                     /locus_tag="ACG8V3_000171"
     CDS             44865..46340
                     /locus_tag="ACG8V3_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0298745.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            46363..46674
                     /locus_tag="ACG8V3_000172"
     CDS             46363..46674
                     /locus_tag="ACG8V3_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298746.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            47035..48291
                     /locus_tag="ACG8V3_000173"
     CDS             47035..48291
                     /locus_tag="ACG8V3_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0298747.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            48288..48833
                     /locus_tag="ACG8V3_000174"
     CDS             48288..48833
                     /locus_tag="ACG8V3_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298748.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            48834..49334
                     /locus_tag="ACG8V3_000175"
     CDS             48834..49334
                     /locus_tag="ACG8V3_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298749.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            49693..49971
                     /locus_tag="ACG8V3_000176"
     CDS             49693..49971
                     /locus_tag="ACG8V3_000176"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298750.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            49991..50335
                     /locus_tag="ACG8V3_000177"
     CDS             49991..50335
                     /locus_tag="ACG8V3_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298751.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            50354..51265
                     /locus_tag="ACG8V3_000178"
     CDS             50354..51265
                     /locus_tag="ACG8V3_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0298752.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            51344..52336
                     /locus_tag="ACG8V3_000179"
     CDS             51344..52336
                     /locus_tag="ACG8V3_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0298753.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            52333..52551
                     /locus_tag="ACG8V3_000180"
     CDS             52333..52551
                     /locus_tag="ACG8V3_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0298754.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            52564..52974
                     /locus_tag="ACG8V3_000181"
     CDS             52564..52974
                     /locus_tag="ACG8V3_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0298755.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            53026..55554
                     /locus_tag="ACG8V3_000182"
     CDS             53026..55554
                     /locus_tag="ACG8V3_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0298756.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            55570..57780
                     /locus_tag="ACG8V3_000183"
     CDS             55570..57780
                     /locus_tag="ACG8V3_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0298757.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            57788..58879
                     /locus_tag="ACG8V3_000184"
     CDS             57788..58879
                     /locus_tag="ACG8V3_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0298758.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            58900..59442
                     /locus_tag="ACG8V3_000185"
     CDS             58900..59442
                     /locus_tag="ACG8V3_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0298759.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            59637..59894
                     /locus_tag="ACG8V3_000186"
     CDS             59637..59894
                     /locus_tag="ACG8V3_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298760.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            59908..>60238
                     /locus_tag="ACG8V3_000187"
     CDS             59908..>60238
                     /locus_tag="ACG8V3_000187"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298761.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
BASE COUNT        20351 a         9907 c        12914 g        17066 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4381 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4441 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4501 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4561 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4621 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     4681 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     4741 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gatcgtttag
     4801 gaggaaacag atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa
     4861 aaatgcaacg ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg
     4921 gacgcttata attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg
     4981 agcatttgtt gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta
     5041 cagtaaagat atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga
     5101 tatggtacag cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt
     5161 tgcggttaca ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc
     5221 gaagtctttt taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta
     5281 tacacaagca aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa
     5341 gaaggaagga agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc
     5401 gaagaaccag aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa
     5461 ggtcgtattc tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac
     5521 acgactgcct atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag
     5581 atcgatccaa gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt
     5641 gtaagtttaa agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca
     5701 ccgcaagaat ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat
     5761 gattatgaat atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt
     5821 cgagagcttc cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg
     5881 acctactgta ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga
     5941 ttcaaagatg tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc
     6001 caaggagaat tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc
     6061 aaccaagtag ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga
     6121 tatatcaatt gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa
     6181 gaaaaacatt taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt
     6241 caagaccatg gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag
     6301 aaaacaatag ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg
     6361 tgatccacat gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt
     6421 ttgaaaaact tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc
     6481 tcaatgatcg aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag
     6541 aaacaggagc catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat
     6601 caccggagga ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc
     6661 tgattcaccc aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat
     6721 cgagatattc agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa
     6781 attatctttt tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc
     6841 taagagactt tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga
     6901 caattgtatt tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc
     6961 tctggattca ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc
     7021 atggggttgt ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag
     7081 gaccttggca tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct
     7141 ctttttattt ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat
     7201 gatagaataa agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa
     7261 ccagacgatc attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac
     7321 tactgtactt aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa
     7381 agaaatgact ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta
     7441 tcttgaaaac ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa
     7501 actgactccc gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga
     7561 tttgtatcgt ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg
     7621 acttgaacca ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc
     7681 agaaaaatat attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc
     7741 tcatgagatt attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc
     7801 ttatacgtat aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa
     7861 aggcgtatat gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg
     7921 aatattagct actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga
     7981 tgaagcagca attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc
     8041 ttcttcattc gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat
     8101 cgagcgagaa ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt
     8161 cggcgaaaac ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg
     8221 atttgatccg gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt
     8281 tttagcaatc gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc
     8341 tggaccagct tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa
     8401 tggtacagcc agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca
     8461 taaaacctat tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat
     8521 tgcacgaaaa gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg
     8581 tcgcctgcaa gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg
     8641 ccagtaccaa catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga
     8701 aacagcggtt aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca
     8761 tatatcagga ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg
     8821 agaattcacc gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga
     8881 acaagcaatc ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat
     8941 ccatcccgaa agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa
     9001 agagcttggt acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc
     9061 tgaaagccta tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc
     9121 tggacgagat atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat
     9181 ggaagacttg aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg
     9241 cgcatttgtt gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa
     9301 aaaattcgtc aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat
     9361 agaacaagtc gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga
     9421 gtaacaatga ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga
     9481 tatcattaaa aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac
     9541 gtacaactgg cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat
     9601 ttgaagagtc agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc
     9661 atcttcatct agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga
     9721 aaaaaacagg agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt
     9781 attatcgatg ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca
     9841 aatatcgttg tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact
     9901 ttaatgaagc acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga
     9961 ggaatctaaa agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt
    10021 ttgaggaact gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg
    10081 ataaagcagc acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg
    10141 tggggcatgc gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag
    10201 atggcatgag tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg
    10261 ataaggcttt tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac
    10321 tggagattct tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga
    10381 cactgcaacg caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt
    10441 tcgcgaatgc tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta
    10501 cagcaaaaga actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag
    10561 aaatggaacg tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc
    10621 ctcacattga tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa
    10681 aaacggattt ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt
    10741 tggttgaaat tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa
    10801 agaatggacg ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat
    10861 tttgttggta tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt
    10921 ctaaatgtcc aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa
    10981 gcaaatatca cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt
    11041 gcaggaaatg catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg
    11101 gaagtgattc gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct
    11161 gtaacaggct ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt
    11221 gtccgtccaa caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa
    11281 ttcttttcat ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat
    11341 gcaatcatga ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta
    11401 tttacagctt tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac
    11461 gatgcttatt tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa
    11521 aacgatgata tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc
    11581 tatcatgggg atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc
    11641 ttaaatgctt taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt
    11701 gcagaagaaa tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca
    11761 gacatgaccg tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat
    11821 ggcgcacgcc aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt
    11881 acgcgtacga ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt
    11941 tacttatgcg atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac
    12001 cttggagcga aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg
    12061 ttagattatc atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa
    12121 caagcgtatg aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc
    12181 agtgtcctta taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt
    12241 tgcaacttat gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat
    12301 caaaaacatg agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt
    12361 ttcacgacct ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta
    12421 cgctactttt ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac
    12481 ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta
    12541 tccttaacag aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat
    12601 gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc
    12661 gttccatcag tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga
    12721 ccaggatcac atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa
    12781 acactgactt ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt
    12841 tctgtagaag cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa
    12901 ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa
    12961 tccaagtaca tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg
    13021 tatgggactt gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca
    13081 attagcaact tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact
    13141 gattttagta atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg
    13201 atttattgcc tactctcttt tgaatggcat aacattagcg gttactcttg catactacga
    13261 tattggaaca gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt
    13321 tgtcggagta acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt
    13381 aatcggagtg atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga
    13441 gctgttcatc tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca
    13501 aagcatccgt aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt
    13561 catggcattg aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt
    13621 tggacggaac aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg
    13681 tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact
    13741 ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt
    13801 gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac
    13861 ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa
    13921 gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa
    13981 atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg
    14041 ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat
    14101 gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc
    14161 gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat
    14221 atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa
    14281 tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct
    14341 gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa
    14401 tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa
    14461 gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat
    14521 acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat
    14581 aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc
    14641 gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag
    14701 gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca
    14761 gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta
    14821 tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa
    14881 gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga
    14941 atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat
    15001 atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac
    15061 ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg
    15121 cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa
    15181 aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg
    15241 gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct
    15301 gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg
    15361 aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa
    15421 aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca
    15481 ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc
    15541 gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca
    15601 ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga
    15661 ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt
    15721 tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa
    15781 catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta
    15841 tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg
    15901 aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc
    15961 cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa
    16021 tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac
    16081 cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa
    16141 cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg
    16201 atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt
    16261 catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg
    16321 aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg
    16381 ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt
    16441 gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt
    16501 tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac
    16561 cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta
    16621 gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg
    16681 aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt
    16741 tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga
    16801 caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc
    16861 ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag
    16921 caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag
    16981 atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca
    17041 aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg
    17101 gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag
    17161 cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga
    17221 aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg
    17281 caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc
    17341 caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac
    17401 tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt
    17461 gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga
    17521 aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca
    17581 aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga
    17641 aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca
    17701 gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag
    17761 tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg
    17821 aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg
    17881 agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc
    17941 attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct
    18001 caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga
    18061 aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa
    18121 gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct
    18181 cccagaaagt gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta
    18241 tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat
    18301 gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta
    18361 agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg
    18421 ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg
    18481 acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat
    18541 cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat
    18601 aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt
    18661 cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga agctgctccg
    18721 ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga
    18781 ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc
    18841 aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt
    18901 ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca
    18961 agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg
    19021 gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg
    19081 aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag
    19141 aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact
    19201 aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc
    19261 tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc
    19321 gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa
    19381 agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa
    19441 agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg
    19501 attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa
    19561 aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca
    19621 agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga
    19681 agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc
    19741 aagtcttgat gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga
    19801 tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc
    19861 tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa
    19921 agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa
    19981 cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc
    20041 aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct
    20101 tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt
    20161 gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc
    20221 acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca
    20281 agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg
    20341 acgggaaaag cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga
    20401 gaaaaaagca gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat
    20461 ctagtggaag acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa
    20521 aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc
    20581 gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa
    20641 ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat
    20701 gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca
    20761 ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca
    20821 aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg
    20881 agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa
    20941 ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg
    21001 agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt
    21061 gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt
    21121 tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac
    21181 gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca
    21241 tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa
    21301 gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa
    21361 atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc
    21421 tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc
    21481 gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta
    21541 tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag
    21601 gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga
    21661 gacaaagcat atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa
    21721 cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt
    21781 cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa
    21841 atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga
    21901 tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac
    21961 caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt
    22021 taaggagata tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata
    22081 attatctaga aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt
    22141 gggtgacttt gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt
    22201 acccgttgca ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca
    22261 aggtccttat gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac
    22321 cgtacgcctt gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac
    22381 gattgcgggt atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca
    22441 acgcttaagc ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg
    22501 atcttggctg caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa
    22561 aaaaatatat gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat
    22621 ggagctgatg ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg
    22681 gacaagagag cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca
    22741 agaggcacgt caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc
    22801 atgacaatca gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta
    22861 gctgaagaag gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga
    22921 gtaatgatga cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc
    22981 gatttgctga ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat
    23041 cagccacaag aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca
    23101 cacacgaaca tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt
    23161 attgaagtgg agagttattt agtaaagact catgagattc attataaaaa actggccgtt
    23221 tatgtacctg tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt
    23281 acacaaggtg attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg
    23341 gaagctggtg cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca
    23401 aaagttgagg ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca
    23461 gctcatcctt atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg
    23521 tttgggttag gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa
    23581 caagtaaaag aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa
    23641 tcctctgtga agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca
    23701 atcgcccaaa gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat
    23761 atgcaaagcg ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag
    23821 caacgattta tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt
    23881 ttcgtttctg aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt
    23941 acgaaaatct tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa
    24001 caagcaaaac gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag
    24061 aattagaaga gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg
    24121 gaacattacc aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg
    24181 atacagcaga ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg
    24241 agtctacgat cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa
    24301 atttgaaaaa ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag
    24361 cagatgacaa atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc
    24421 cttctatccc tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg
    24481 gagcggataa attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg
    24541 gtcctttagg tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc
    24601 aagggaaaaa tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag
    24661 caatcgattt tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg
    24721 aaggtttctt ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata
    24781 tcattcgcga tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc
    24841 aagaagagtt gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat
    24901 attacaacat gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag
    24961 caatgatcga gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt
    25021 caaaaatctc ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc
    25081 acggacgatt cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa
    25141 aaattgcaga attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt
    25201 tcgtcccaat gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt
    25261 gtcccgccct cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt
    25321 ttataacaaa agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta
    25381 tgtaaaagaa aagcagccag aattatatgt agaactagct gaaactggcc catcgattga
    25441 tgatgcggaa ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg
    25501 tggagacggc acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca
    25561 tgtcggatta cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct
    25621 caaaaaagaa gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg
    25681 tcaagtcaat gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc
    25741 agtttggatc agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt
    25801 ttttaagtta ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca
    25861 ttggaaagga aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac
    25921 aaattttgac gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc
    25981 cttagatatt ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac
    26041 gatccccggc attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc
    26101 tgtgggcact cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt
    26161 atcaaaagga ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt
    26221 aacagaaagt tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc
    26281 ttttcattgt gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa
    26341 ctagcggctg gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt
    26401 tgggaggatt gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta
    26461 taggattcgc tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag
    26521 caggagatgt cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt
    26581 gtgatttttg ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa
    26641 ttgctgtcta ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa
    26701 atcgctgaag ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg
    26761 aaagatgggt tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca
    26821 ggaacgctga tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga
    26881 gcaggggtga cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta
    26941 gctggcttgg tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt
    27001 atcatcgctg gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc
    27061 agcttgaatg ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt
    27121 ttgcggaaat gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa
    27181 accattatcc aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt
    27241 gtattttggt atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt
    27301 ctactgacca tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct
    27361 gctggtattg gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca
    27421 gttgacgcag tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc
    27481 tatggatttg tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa
    27541 gtaaaaaaag agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat
    27601 caaatcaaag aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat
    27661 atacatacaa tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa
    27721 actatgtgta tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca
    27781 aagaaataag tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat
    27841 gactacgacg cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca
    27901 tcaagatatc gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg
    27961 aaatttctat acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct
    28021 actggatgag tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca
    28081 gaatttcttt cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt
    28141 tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt
    28201 ttatttgaat aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag
    28261 aggaggagaa agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata
    28321 cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga
    28381 tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct
    28441 gataggagaa cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt
    28501 acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc
    28561 attgatcgct ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa
    28621 agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt
    28681 aggggcagga aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc
    28741 tcgcggtgaa ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga
    28801 aaaagacaaa gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga
    28861 agatacgatc agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa
    28921 catccatgac aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg
    28981 gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga
    29041 aatgaactct atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat
    29101 cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt
    29161 gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac
    29221 agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca
    29281 cgaattagaa gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg
    29341 tacgatacct caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga
    29401 gattaagatg gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt
    29461 aacgggtatc cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa
    29521 tgaaacactt cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc
    29581 ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt
    29641 cctaggacca actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt
    29701 tgactcagaa gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt
    29761 gtctcgtcta gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac
    29821 ggaagctgtc agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca
    29881 tcccgatgtc ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa
    29941 agggcgagta gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca
    30001 gttactgctg gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt
    30061 caacacgcta ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat
    30121 tttatttaca ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca
    30181 attggcacaa cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac
    30241 gtggattgct gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat
    30301 cactaaggaa gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa
    30361 atcaaaagta acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga
    30421 agaaatcgtc taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt
    30481 gtcagacctc cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc
    30541 cgaaatttct tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt
    30601 tttctaattt ataactttca aagtaacgct catctttttt actttgagac catgcaaaca
    30661 atcccattaa ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg
    30721 ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta
    30781 agaactggtc gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg
    30841 caaagccgaa taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca
    30901 agtaaccaat caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga
    30961 ttttgtccca aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga
    31021 tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt
    31081 tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg
    31141 tgattccttg agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa
    31201 cgaatacacc aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa
    31261 gtccctccat ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact
    31321 tttccgttca atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca
    31381 gctgtttctt gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca
    31441 gtatataagc cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc
    31501 ccggcatcga tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt
    31561 tctgtgtcag caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct
    31621 tcattttctt gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact
    31681 aactgttggt tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc
    31741 atctaaacat catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat
    31801 gatacgctct agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa
    31861 ataagaagca cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac
    31921 ggattttcct ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc
    31981 gaagacacca ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc
    32041 tgttccaaca aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa
    32101 atatggttcg atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt
    32161 tttataaata tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca
    32221 taagccattt cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc
    32281 agctacccag tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc
    32341 tgtgcgctca tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa
    32401 taaagcacca tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc
    32461 aactggtaat cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct
    32521 gattttaaag gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat
    32581 actgtaagga tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac
    32641 gataattaga tcttgcatca atccatggtt agcatattct ggataagttc caactccagt
    32701 cacaccaccc attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat
    32761 ttgagagatc agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg
    32821 ttttttggaa cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc
    32881 agtcacttgg aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac
    32941 cggttttaat tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat
    33001 tagcttgata acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa
    33061 aactctttaa attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag
    33121 gagacgctat ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga
    33181 tcatcgctca tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc
    33241 acactgtgac gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg
    33301 aacgtggaat cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag
    33361 aaacatatat ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct
    33421 ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg
    33481 aagcacaaac attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag
    33541 tcatcaataa aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag
    33601 acgtaatcgg aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga
    33661 ttgaagatat tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg
    33721 caccgctaaa agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa
    33781 atgtccggat cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg
    33841 gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact
    33901 ttttgatggt cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac
    33961 gagtagggga tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc
    34021 gaaaaatgaa tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg
    34081 atttgcggga agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag
    34141 aaacctctca agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg
    34201 atgtcgtcca agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat
    34261 ccgttattta ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag
    34321 attttcctga acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca
    34381 tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat
    34441 tcattacgat ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt
    34501 ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat
    34561 tggattatga aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga
    34621 atggagaaaa agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg
    34681 gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa
    34741 tccaagcagc tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa
    34801 acgtcttggc taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac
    34861 aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct
    34921 tcatggccgt tctgaaaatg gatgagcaag atagtgtgaa ataacttata atcaatcgaa
    34981 aaacctatta aataaacctt tttgagtaga tgcaacactg aggattatct gtataaaaga
    35041 ttttaaactg tcatttggtt atcacggcga tactaaaatt aacaaaattg gaatattttt
    35101 cttgaagatt tttagctgtc agtatatgat gaaagcatct cataggaggt gcttttttca
    35161 tggttgataa ttcaagtgga agggttctta aatctctaag aaaagagaaa aaactatctc
    35221 aaaaaaagtt agctgattta gcaggtattt cacaaagtac tttagttaaa tatgaaaaag
    35281 ggtcaagaaa aatccctaaa gacgtagata atactctaag caagattctg aatatagaaa
    35341 ctctaataaa agatgaggaa gataagattg agattctcat cggtaaacta attgcataca
    35401 gagatatgaa taagttactt aacaaagagt tagctgataa tataggaatt agtgaagtat
    35461 tactcagtta tgtgttaaat agaaagagaa atccgtcaaa agaaatgcaa aaaaaaatcg
    35521 atatttttct attaagtaat gaaaaagaaa ttttgaagga gattaatcgt gatagtgaga
    35581 tatttagttt atcaaaagac gataaaattg ttatgggaaa aagaatacga gaagtccgaa
    35641 agaaccggga agagacgctt gaaaaatttg gtaaaaactt taccatctat acaggcaaaa
    35701 atgttataag tagatgggaa aaaggtatta atattccaga tattgaaaaa ctaatgaata
    35761 ttgcctatct tggtaaggtc actgttcctt accttatgta cggagaagat tataagaata
    35821 tacttccaaa agatgaaaga gttagcgatt ttaaaaagat aaattctttt agtatgggat
    35881 taaggatgag gaaaattagg aaagattatt atctagaaag ggaggagttt ggaaagcttt
    35941 tttctccttc aatttcaaaa tggtccatag ataggtatga gaatggtaga gatataccca
    36001 atactaatcg aattatccaa tatgcttata taggaaatct ttctttagaa tttttgattt
    36061 acgggatata aaaatagttt acgtcgatac ttattaagtt atcgacgttt tttattatag
    36121 aaaaaattta taaaaggagg tttcttgata tgataagaaa caaaaagggt ttgttgaaag
    36181 ttgctttact ggcagctttt ttctaagtaa ctttttgaca agctcaatgg caacacaagc
    36241 ggaagaagta atacaagaac taactgttat gaataacagc tagataaaat gggctatgta
    36301 tgttatccaa caatttagta atcactagaa gcctagcgaa agttcgcttg ataagttata
    36361 gatgagtatt gatttgtagt gtttccaaat caaaagggta cagagccaca tgaaatatgt
    36421 tgtcctctgc agcgtcagga tgggaatcca taccatggag attcggagat ctgtgaggga
    36481 atacttacgt ggttaatatg tcagttgtgt tggacgtagt aatggtcata gtgggtagat
    36541 tgactgaatt cttatatgag aaaatgaaac caacaagaag atagggttat ttatacagaa
    36601 ttggggatct tttctggaaa agtaatcgga taatgcgaga ttcgtacggt cttttttaaa
    36661 tttctctaaa aaaaacggca tcgaatgggt tgggtgattg ataagcaatg aatcatagac
    36721 tgatatagaa taaaaaatga ttctatttat ctttttacat ctatcgtttt ttcaagtagc
    36781 cgcaaattat atcagcattc aacaattttt gtatacaagt tctagtaaaa ggatctgaag
    36841 aaaaagttgg ttgttgtgaa gataaaaaaa gaatcaggtt ttactgagtt tttcttcaaa
    36901 atttgtgtta ttaggttagg acaaagataa gaagaagtat gaagatttaa aaaaggaatg
    36961 aggaaaagat agtaatttcc gattattgga gtttttttat ttttcgtttg tcaaattaag
    37021 ttagacaaat catcttttgt gacgtaactc aaaaatactt ccattggtgt ccgatagtgt
    37081 aatgattttc taggaatatg atttcgtttc gatgccaccg attggataaa ggtttcagat
    37141 acaccattaa aatccatagc tttatacaaa ccatctttac gtaataagcc atttgaatgt
    37201 tcattcaatc ctcgttgaga aggtgttcct ggatccgcaa aataaattga aatatcgttg
    37261 gtattactaa tctttttcca atttgagaat tcttttccac aatcaaatgt gatggattta
    37321 aataagtttt ttgggacaca acgaaaccat tgattcagcc tattttcaat gtctttagcc
    37381 tgtctgcctt cgggtttcaa ggtaatgatg actttagata gccgttcaac taatgtgatg
    37441 accgcgcttt tatgttttcc acccacgata gtgtcacctt ccaggtgacc aaattcatga
    37501 tcaaactggg tatagtctgt ctttctgtct cggattgacc ggcgaaaccc ttgttttcct
    37561 cttttttctt tatgaccgtt tggttttctt tttcccttca ttggtaaatc gtgagtgtca
    37621 aaggttttat ctttaaacat tcgatacagt gtgcgtaccg aacaagcaat aggaaactct
    37681 gctcgaccaa taaggacatc aggagcccac ccttgaacaa cctttttttg aatgtattcc
    37741 tgttgctcat caggtaaaac aatcggatgt cttccgcagt tacgtttgtt tttcttatat
    37801 tgtttatagt agctcattgc gcttccacca gatttaagaa aacgatagac cttataaata
    37861 gtttgtcgag aacgttgtaa cagattggct acttttgaaa caggttgtga ttgattgaaa
    37921 taagcttcta tcattacgag ttcagttggt gtaagatggt tataggtcat ttgtactcac
    37981 tccttataat tctttcgtcg gaactatttt gagtgtagca caaatgactt tttttagttg
    38041 tctagcttaa ttttacaatc ggcgatttat aaagaagaag cgaataagtt gtgattccta
    38101 gatttcgcgc gtgattaatt aatgagcaaa aaatgcggaa agttttagtg tttaatacac
    38161 gaggacaaag tttcttaggt attgaaggca cacgtgtaac ggttgatggg tggtgtgatt
    38221 gtttcgaggt tggaaaacat gttgaactta tggcatttac tgagctaaaa gataagaaca
    38281 acgtggaatt tttcgagagt gatattggtt gggacgatca tgaagaagtt tatggaaaat
    38341 ttattttgcg gaagggaaat tcttatacga atggggaagc ataagtagcg atttgttaga
    38401 gatcattgat aggattgaaa ttgtcgggaa tatttatgag aatctagagt tatttgaggt
    38461 gatagaatga gtgatggttt tgaacaagta tcaataatta aaaatcaagt tcgggagatt
    38521 cttagaaaaa aaggctattt ggtagatagt tattttgaag gagattacga aacatgggta
    38581 ggtgtctatg ctcgtccaga gaataaacct acctatttag atcctacgac gtcagaagat
    38641 gcctatttac aaaataggta tcgagtagat ggttttaagc aagactttgc ggagtggttt
    38701 gaatgggaaa ttgaaaacgg agaagtaaaa gaagaataag agtacttgtt gtatcaaaat
    38761 aagccagtag aacaaccaaa ccagtagcta gtaatgacaa taatactgag gaatcggatc
    38821 attatggtat gattgatatt tctcttccag aagatcttaa gccagatcct gtaacaattg
    38881 gcaatagtgt catgattttt gattcattag atgaagctat gtcatatggt tggaaagaaa
    38941 ttaaggatga aggtagtaat tggtatggca tgagatttgg tgatggtgaa gttgccaaaa
    39001 aagctggtat tccaattggg aaatggacag ttgatttcca cgaagcaggt tctttttttg
    39061 ttctgtaatt atagtgggag ggtaaaatca ttgatgaaat gtgttcagat aagagcgcca
    39121 gataaaaata gaagagacag ttcacgtcgt tatcactttt ggtaacggcg ttttgtttta
    39181 ttatataaaa ttctcaggaa aaggaggaat tcttgtgtat gcaaaaacga aaatgaattg
    39241 gttgaaagca ctcgcattgg tggctttttc tcaattctgt ttcttggtgg gaactgctca
    39301 agcacatgca gaagaaacga cggctataca acctacgatg gaagccattg cccaagtcgc
    39361 ttctgaagtg acagaagtcc aaccagcaga agccggggtc ccagctggtg aagccactgt
    39421 tgaacaggcc cctgtgacag aagcgccaac ggtcaattcc aaaacgactc tcgatcagac
    39481 gattggggaa acacaacacc cagggaatag tcctggtact tcacaaccag ttgaagaaac
    39541 tgcctcaatt acaacccctg cttcagaaac gtccactcta gaaggagaaa aagctgccga
    39601 agcaacgcct tctgtgcagc cagagtcttc tgagaaacaa gcggaagaaa agaaacgaat
    39661 agaagaaaca gtggaacaac cagcggctac tgctgacaaa gaaggaaaaa atgaggaaaa
    39721 cgttgaaaaa acaatctctt caaccgcagc tagtcaaaat caaccaacga aaccaaacaa
    39781 agaatctacg gctagtattg aaaatcaaaa gaaaaaagag cagaacattc caaaaacagg
    39841 tacacctaca ctgttagtaa cagtaccaac caatcgttcg gttcgtgttc gccgtgcaac
    39901 caatgcagct gttttaggtg gtcggaaaat tgattggggc gaaaatattg gtgttacttc
    39961 tagtccgaac aatctaaact tcactttaaa ttatatggtt cgttttgaga ttaatggaca
    40021 agtggcgttt tgtattgagc ctggtgtctc agccgcagaa ggtcaagttt atacagctac
    40081 tgctttgaaa aactatttga aaaattcaaa tgtacggaaa aaaacaagct tgatctctta
    40141 tttaggatat gtcacaaata aagacaaatc cgatgaacaa tacattgcgg cacaatttat
    40201 gatttgggaa gctttgggca caaaaatccc aatcaaaaat atccgtgttg attatgatcg
    40261 tcgaaaagca gaaattcaac gtttgattaa taactttggt gaaaaagcaa cttttaataa
    40321 tcaaacacat acggtaaaaa ttggcgaaac gttgaaagta aagaataaaa atgatttttt
    40381 gaaatatgtg aaagacattc aaaaaccaaa aggtgtcaat gtatctattc acggcaatga
    40441 agtatggatt acagtcacaa aagaggcacc ggataaagca aaaattcaat tgaataaaat
    40501 taaagttcaa ggtgcgccat tagcctatcg tcgtccagga gctcaaacaa ttggtgtgtt
    40561 acatccaaca gaacctaatg cgtctgttat taatttacaa attctaaaac aaggaaacat
    40621 tcgtgtgttt aaagaagatg cggaaactgg tgtgaaagca caaggtgcag cttctttaga
    40681 aaatgcggtc tatggtttgt atcaagcaga tgggaaaaag attaaagaaa ttaccttgaa
    40741 aaaaattaac ggcaaagtac aggcagaaat taaagatctt gatcctggca aatatgtttt
    40801 aaaagaaatc aaagcgccaa aaggttatgt gctttctgat gaagcaattg aaattgttgt
    40861 tgaacctgga aaaactgtca atgcgaccgc aaaagatcaa gtgatcaaag gaaccattga
    40921 tttagtgaaa gttgctaata aagatttagt cgattcaacg aatccagata acaaaccaaa
    40981 attagctggc attgaaatca ctttgacgtc aaaaacaact ggcaaagtgg tgaaaaccgt
    41041 tgtgacagac aatgatggtt atgctacttt tggtaaaaat accgttgtct ttgatacgta
    41101 cattctttct gaaaccaaag gaaaagaagg gtataaacta tttgaaccac ttgaagtcac
    41161 aatttctgaa aatgggcaaa ctttccatta tgttttagaa gataaagtaa ttgaacaacg
    41221 tcttaaagtg gtgaaagtgg atcaagaaac aggaaaagtg attccacttg ctaatacaga
    41281 attcaagatt tttgatactt tagccaatcg ttatgtcaca atggagattc caaatgatac
    41341 agaaacaacg gatatcttta aaaccaatga aaaaggcttt ttcctaacta acggtacact
    41401 tacttatgga aaagatcgct atcgtttgga agaaatcaaa gcccccgtaa attacgtttt
    41461 gaatcaaaac ccattagtgt tctcgattac caatgataca gaaccgatca aagtgatcca
    41521 ttttgcgaac cgtcaagcac ggaaaagtgt aaaattattc aagtatgaag aatggaacaa
    41581 gaagaaaacg ccgctcgcag gtgtgaactt taccttgtat aacggtaaaa atcaagcaat
    41641 tggtgaatat accactgatc aaaacgggga aattttagtg aaaggtctag ttgccgatga
    41701 ctattacttc ttggaaaatg ccccattaga aagtttccaa ccaaatacag ataaacaagc
    41761 attcacggtt gcattcaaac aaaatgcgcc agatgatggg gaaacgttag tggtttctgt
    41821 acgtaattac ttgattccac caaaactaga aacacaagcg tcaaataaag ccgatggtga
    41881 aaaagtggtt gatccattag aaacgatcgt cattaaagac agtgtttctt ataccaactt
    41941 gtttgtcgga aaagaataca ccatttcagg agtattgatg aacaaggcaa cgaatgcgcc
    42001 aattttacaa gatggcaaag aaatcacaag taccttggcc tttattgcag acaaagtcaa
    42061 tggcacaaaa gaccttgaat ttgtggttaa tgctagtgtt ctgaaaggtc aaacgatcgt
    42121 tgtttttgaa aaacttttcc gtgatggcat tgaagttgca gcacatgcgg atatcaacga
    42181 tgaagcccaa agtgtgaaag tgaataatcc aaccgttcat accacggctg tctttgaaga
    42241 cggattgaca gtcgcagacc caacaggatt gtttacctta aacgataccg ttgaaattac
    42301 cgagattctt ccaggaaaag catacttggt caaagggatc ttaatggata aagcaaccgg
    42361 tgaacccttg cttgtgcatg gaaatacggt agaaacgagt gttggcttta ttgccaaagc
    42421 agaaaatgaa acggtgatcg taccatttac ctttgatttg acaggtataa atggtcgtga
    42481 aatcgtcgtg tttgaaagtc tttttagaaa tgatgaagaa ctagcaaacc ataaagacat
    42541 caacgacaaa gggcaaacgg tacgagtaac taacccaagt gtacgtacaa cggctacaca
    42601 taaacccact ggcttgaaag aggttaatcc attaacgaaa gtcaccatta cagacgctgt
    42661 gaaataccac gatttggtcg ttggaaaaac atacacagtc aacggtgttc taatggacaa
    42721 agtaacggaa aaaccagtgc tagtagatgg aaaagaagtc acaagtacct taacgtttgt
    42781 ggcagaaaca acggatggta cagtcaatct agactttatt ctagacgcac gtgcgttgcg
    42841 aggcaaagaa gtcgtggtct ttgaagatct gtttagagaa ggcgtattat tggcttctca
    42901 cgcagatatt accgacaatg accaaaccat caaaatcagt aatccaacca ttacgacgaa
    42961 agcgacgaat aaaaatggcg gaaaagagat cttggccttg ccaaatcaaa ctgtgcttga
    43021 atgggtcaaa gtagacggtc ttgcgattgg tcaaatctat cgcttaattg ttcaaggcta
    43081 cttaacacca gacggcacac catttgaagg cactcatgcg gaaaaagtct ttgcggccga
    43141 taaagaaatg atggaattct tatttgaatt cttagtggac ggtcgcaatc ttgcaggaaa
    43201 aggcttgagt tttgcggaat ggctccaagc gaaaacggaa gatgagaaag aaacgtttga
    43261 agaagtggcg aaacacaatg tagatctaac aaacaaagat caaacggttc aaattgtgga
    43321 agcgccaaaa ccaccgcaac cagtgaaacc agaacaacgg gttaaaccag ctactgttgt
    43381 accaatgttg ccaaaaacag gtgaacaaac aagtagtata cttatggttc ttggattagt
    43441 agtattagtc attgctttag gtataacact ttataaagta cgtaaagcca aagattagtc
    43501 atgaggaaca agcggttagg atttttaacc gcttgtttga ttgtaatgta gaaatattta
    43561 gaatttattt tggtttttaa gaggaggaaa aactttatgg cagaaaaagc attgcgtatg
    43621 aagaaagagt ttttaggtca agtattttta ttgggttcac gggaacctaa tccaccaaag
    43681 gaaattaatg gtgtgtctgg gcgatatgtt caattacaag cagagtgttc gcgagaagaa
    43741 cagcctgctt tttttgttta tctcacagct gaagaggata aaagattacg attaccagaa
    43801 aaagaggtgc gtatttcatt tgaagaagct tattttgttt ttcgtaaaga acgagtgaat
    43861 agtcgtgaaa caaatgatat gggacaaaca gagcgtcgaa caactggcta tcaagatgtt
    43921 ggtgtctttt taactggtgt aaaaattggg gaggttttgt aagatgggat taaaatttcc
    43981 aaataattat gacattacag aagcattaga tgaaacttgt ttaggagccg tacgctttgg
    44041 cgcattagaa gagtctgatc gtgtatttga taatgaaaca ggcgattata cgggagaaat
    44101 tcgatcacag cgagcacaat tagtttttgg aaatccagat gaaccaaaga ttgcacgacg
    44161 agttaaattt ccagcagatg tagatttgac tggttttaaa cgacgtgatc ctgtgaaatt
    44221 aattgatcca gaatatcatg tggtatatat tccagcacaa gatggcaatc gtgaatattc
    44281 agatttagtg atttatgcga aaggattaga gaaagtagaa gctccaacaa ataatcctaa
    44341 tatgaaacaa tcacaagaaa aaggtttaga tcaaaaacaa ccaagtgttg attctaagga
    44401 aaaagagaaa aaataaggtt tttttgaaag aataggaagg agtcttccta ttctttttat
    44461 ctaagataga aggaagggga gattttgaat tcagataaaa ccaatcaaat actttgagag
    44521 attgtgcttg ttatcaaaat atccaacaat tgattgagta ttttggagac aatccaacga
    44581 tttttgtaca aacaaatgca tatatttgtg cggttattgg gcagtgtatg tttttacgtg
    44641 atcttgttcg tttttcatta ccgtaccagt ggaaacaaga tcatcaggaa gtgatttgga
    44701 ttcgtatagt acgaatatgt aatatgtgtg cacaccctta taaaaatcga ttaaatcttt
    44761 ttcgattatg tttttttact aaatgtgata ttcctgtatt agttgctttt tgtcaaaagt
    44821 ttttagcaca ggaaaagtaa aaacagaaag gaggaatgtg acaattgaaa gctcattttt
    44881 tcaagtatcg tggtcggcga attcgctatt tcaatcgtta tttatggttt tgctattatg
    44941 gtttcttttt gtttccattt ttgattctgt taggttggtt tagttggaaa gtattttatc
    45001 cacgtttaag tacgttttcg aattggcaaa tgtatcttat tcctggaata agtattgtgt
    45061 tgtatttgtt gctggttagt gggttaattt tagggttgat gcattggtcg cgactaaaag
    45121 aaggatattt tgcctctgtt tattggcgtc agttacttgt tcgaatgtta attgacaatc
    45181 ggtttgttta tacaaagaaa caagttagtg agaaacaaac aagggaaaaa atgagattac
    45241 caaaagttta tttttatcaa aagaaacaag aattagtggt ttcttttcct ttagatggtg
    45301 ggcgttttca cgaacgattt ttaaaaatgg gtcatttgtt atctgaagta tttttgcgtg
    45361 atttaattcg tgaagaacga gaaaaagccc gaatcaatta tgtattcctt tcagattctg
    45421 gtcggattcc tattgatcaa tgtatagcag aaaatggacg tattcattta atgaaaactt
    45481 tggattgggt atatgatcaa tcaccaaata tgttgattgc tggtgcaatc ggtggtggga
    45541 aaacctattt attgtattct ttaattcaag cttgtttaag tgttgggaca gtagatattt
    45601 gcgatggaaa agcggcagat ttagcagcat taggtgacgt cgatgtattc aaaggacatg
    45661 tgttttacaa aataaatgaa gaaatgatta tttgtttacg aaatgcatta aaagaaatga
    45721 atcaacggta tgcctatatg aaaaccatga aagatcctaa atatgaacca gggaaaaatt
    45781 atgcttatta tgggttacca ccgcatttta tcttttttga tgaatgggca gctttttatg
    45841 gttcgttgga tatggaagcg agaaatagag tcgataaatt gattcagcca ttagtattaa
    45901 agggtcgtca agcagggatg tacattattt tagcaatgca acgaccagat gcagaatatt
    45961 ttccaagtgg tgttcgggat aatttaacat tccgtatttc tgttggtcga ttgtctccca
    46021 ttggttattt aatgactttt ggagaagatt ttaaaaataa gccattttac aacaaagatc
    46081 aacctggtcg tggatatgct tttccaggat ttgatgtacc tgtagagttt tttgcgccat
    46141 ttgtttacaa agattatcac tttatcaaag aatttgagaa atttactgaa atgattgtct
    46201 atgattttac agtaaaagag gatagtcaag atcaaacgat gggagatgat aatcagttgt
    46261 tacaagaaca actagagcgc caaatgtatt tagcaaaaga tccaaaagga acaagttttt
    46321 ctgatgcgca tattttgtaa tcaactaagg aggtgaagtt aaatggcaga gagttgttat
    46381 tatagagtag ctacagcaat acgaatgatt aatccttctc tttcttccag aacgttttat
    46441 gattggttga atcgtattga acaagtaact gattaccgct ttttgcgcaa agaacgtgtt
    46501 tttacaggaa aagttattaa tcaagtattg ctaacgaaaa aagatataga acgattgact
    46561 cgattgtatc attatcgagt agatttagaa gaggatttaa ctttgtcaat ttatcgcgtt
    46621 ttttctcctg aaaaatatag tgaaataaca aaattagatc atttaatttt gtagttatta
    46681 tctatcggga gggctctaga cgtagtctgg tagccgcact ctgcctggcg gtgcgcatta
    46741 aggctgcgaa gcatgggcct ataatgcgca ctcgctagca gcgtgtgcag ctcacaaaga
    46801 ggtcgtgcgc ttgcgcacgg cctctttgtg taaagccccc cgatatataa tatggggggc
    46861 agaaataaca taacacatag aaaaaagttt tataaaccct tttgtatcaa gtgattatct
    46921 aaggtcagca gttgtactac ttttcccaaa aaacgagggg gactagttga tatatcggtg
    46981 tttctaaagg gttaaaaaaa atcgattttg ttattttata aaaggagcga gaaaatgaca
    47041 ccgattgagc taaaaagcta cagaaagaag ttaagtgcaa ttcataatat aagtggagat
    47101 aaaaagccgc tatcacaagc aagatttgcg caattgatgg gctgctcaaa agattacatt
    47161 agaaagatgg aacaagggaa aagaaaaatc ccagaggatt ttgaacaacg aatcaaggaa
    47221 cgctttcgat tgtatatgaa aagcggaata gatcttcgag caatgattga ttatttgcgt
    47281 ctttcctttt ttgggacaga ctacaaaaca gtgattcaac gtgtattacg aatggatctg
    47341 acagaatttg aaacacgtcc aacgggcttg tatctttttg actgtctttc tgttcgtggg
    47401 aatatctggg tgttctggca ttcaaaacga actgtaacag aaaatgtact tgtgcaattt
    47461 tcaggacaag gttgccggga atatgaggaa gtcatgcgtg aacaacaaac agattggcga
    47521 acatggctat attccatttg gaaaaatcaa ggaaagttgg aagctggtta tacacgtgtg
    47581 caatgtagcc gattcgatat tgcgttagat gaactttggg gattggaaac ggaagaacat
    47641 ttcgatctct ttgaattact agaaaaacaa aaagcaggtt tattagaaat ggactttaag
    47701 agattccaga atattggcgg ttgtttttta gatgatggct attttcgaag tgaaggatta
    47761 tctttgtatt ttggatcgcg aaagagtcct ctctttttta atttttatga gaaacgtttt
    47821 gaaattgcga accgagaaaa aatttcagaa ttagaagcat taacgaaata tggtatttat
    47881 aatcgatatg agttacggtt agccaatgag aaagcaacac aagcagtaga agcatttatt
    47941 ttaggtgatt caccgaaacg tttaggagag attggcgttg gtttaattaa tgcaaaactt
    48001 attgtttggg atagactgga aagtgatgca cggattgttg ataaacgatg ggcaaaatta
    48061 tttcgttcga tggaagaatt ggattttaca gtaaagccag aaaaattctc attagatcgt
    48121 tcagaacgct ggtttttgaa acaagtagct ccgacattaa agttgttaca agaagcagat
    48181 cgtattttag gaagagactt tgtacaagaa gggattgata atgccgaact aaatgacgag
    48241 caacaaaact atctagaatg gatgggaacg tcaggaggga aaattgcatg aatgcatata
    48301 cgacaacaga atggctgcgc ctgttggatt taaaggctgc aatcgaagca ttaaatgaaa
    48361 agatggttga tttgtcttat tttcgttttc gagttcctta tattgaacaa gcggtaaaag
    48421 ctggacgtta tcaggaaaaa gagaactggc aagaaattgc acgcttatta gaagttcgta
    48481 agggatacga acaagaactc gaagagttgg aatttagccg aaggaaagga aggctagaat
    48541 tcattcgctt ttatcgattt tcattgccaa tacctgcgat tttagcggtt aaaaaaggct
    48601 gcgataaaat gaagatatat gaaaactgtg tggcagcact tagcagcgaa aaacctctta
    48661 tggaagaaat ttcattggtg actgtcactt gggaaatgag tcgccagcca acagaagaac
    48721 agacatatct ttctttagaa gagattgaga tagaattaga ggaaattggt cgctatgcca
    48781 cttgttctac ctattgtgga tcggtcatta gtattgctgg tgtgattgtc taaatgaaaa
    48841 aaattatgat ttatgttggt gcgtattggt cacgtgatcc aacagtatta gaaaatccag
    48901 aggcggtaca ctattgcttg cgccaattat tctatctgta taaagaacga ttagaatcgt
    48961 tgattcgtca gttaccatat actgacagac gtttagatga acttcttcta aggtatccag
    49021 caatgtataa acgacgaaaa aatagattgt taccagaaga gtatcccatt gaaaaacatg
    49081 aacttgaagg gcgatttgtg gcttatttct atgatgatgt tcgtatgcgc ttagtagaac
    49141 aacgaatgga aatagaaaat gatcgctact attttatcaa ttattgtaag aagcgtaagt
    49201 atcaagtaac tgatgatttt tatgattgta ttttacaaga tggggaagtg attctatcca
    49261 aaattgctcc aagttttcgt gaacttgtgc cgattgattt tttgaaaaaa tgtcatttgc
    49321 ggattcttcc gtaaaaaaga gctataaaag gaaagaaaag tagaagccaa atcggtttct
    49381 gcttttttat tttttttagg aaggagaaga tggtatgtca ctggaatttt taggagagcc
    49441 aattgaaaag gaagagttcg tttcgcatta tatggatttg tttattcagg tgataaaaaa
    49501 ctttgtgcga ttgatgaatt ttatccacgc gaacaagact atctagccgc tgaacgaata
    49561 aaaatcagtt tattatggga aaaattcaca gatgaaaata accgaccgcc agattatcgc
    49621 tatttatcag attgtttaac gaataatgtg ctttctaaag aatacctatt tcacaaacgt
    49681 gagaaaaatc aaatgtcggt tgaacatttt tgtgattgtt atattgcatt attgcgtagt
    49741 caacagttat taacagtaga acgatatggt gcagagtttg gttatgtcat gcaagctgag
    49801 cgtcatagag ctacaagatg ctatgaaaaa gaagatcaag tgattgatgg ctatgaagaa
    49861 ttatgtattc gaaacaatcg ctatttgcaa caacgtttat atgaacaatt gatgatggat
    49921 ttttcaaatc tctatagagg gtatgaatgt tcgatgattc aagaaaggta gaagaaaagg
    49981 gggaattttt atgtggcgat tattattttt ggtggtatgt ggtatggcat tgttctatgt
    50041 gtatcgtgtc tatctctatc cacgaatgga acgatggaca agttggtata gagatccttt
    50101 aaaacaacga aaaggattga agcggtatcg ttatgaatta cttgttgaat tttcggatgg
    50161 gcgacaagca cagtattatt tttttgcaaa ctatccaaaa tacaacacca gtgaactatt
    50221 gattcaatta ttcaaaaata atccatatgt tgggttagaa gaaaacggac gggagttctt
    50281 ttatcgttca gataatatta gtgcggcgtc tatgaacaaa acacgaattg cataaaaaat
    50341 tggaggaata atcatgaaaa aaacaacatt agcagtcgta acaacactag gaatttcatt
    50401 attttcgttt caattaggga gttcattggt ttatgcagat gaaactgtag aaacacctaa
    50461 gtcagaaatt gtagaaacat tgccagggga aggattacca gatgttgaga cagagctaaa
    50521 tggaggaaca acggaaaaac cagcagaacc tgaacaattg cagtcagaga aacctgaaaa
    50581 agagccagtg acaccagaac cagagtttcc tgaatcaact ttaccgactg attcgatcga
    50641 agaagaaggt gtgcatagtt caacaacaac ggattcaagt atagtcacaa ctccggaaca
    50701 atcaggaaca accagttctt ctgaaaagaa tactgattcg tcaagtacgg aacagccaac
    50761 gatgtcgtct accagtgaag aacaaaaaga agaaccgaaa caacctgttg aaaaatcaga
    50821 agcaattcca aaagaaaata aacctattgt tccagcaaaa gaagtcacag tatcggtaac
    50881 cccaagcggt gaaattacga cgaatacttc tcaaggaatg agtgtaccga ttgtcactag
    50941 taatgtagag gaattgaccc atattccaac accaaccacc ccattaaaag tagcaggtga
    51001 tcaaacaatt gttggtgtaa aagatggtat tccattaatc caagatagtg aagggaattt
    51061 ggtaaaggat ttgtcgattc ctgtaaagaa acttccaagt ggcaatattg aaatcaaaac
    51121 agcagatgga aaaacaaaag tcttaccaaa gactggtgaa gaaatacatg ttgcactttc
    51181 tgttctagga ggagtgttga caagtattgc tggatttgtt ggatataaaa gaaagagaaa
    51241 aggaatagaa aatgagggta attgaatgga ctatttttca gaaaaacaag cttatcgttg
    51301 tcaatcgtac gtactagtaa aaacatacta gaaggagtag cttatgaaaa aacgacaact
    51361 acggcggaaa gaaaaagttt atacagagaa agtaccgaag caaaagaaaa tccattcaaa
    51421 aaaagcagtg aaatggattt ggtgtttggt tttatttctt tgtttttcag ggggcttggc
    51481 attttttcaa tccatgaaaa cgaaggttca agtgtcgttt ttgaaagatg aaatccaaca
    51541 attacaaaag aaagaagtac aaagtcggtc agaattaacg tacacacctg aaattgaatc
    51601 ctttatgaat cgttttgttg ctttgtatat gaatacattc gataatcatg aacaagaaca
    51661 acaacgtcaa gaaacattaa aaaaagagtt ttatgcacaa aactttattc aagaaccagg
    51721 aagtgtttca ggtgttcgaa aattaactgc tgcttcgttt attggattaa aagaaattga
    51781 aaccattcca actgctagtt ataaagtaac ttatgatatt cgctcgatga atccagagag
    51841 taagaaagaa acaagtgtga caatcacaca agtactcaat cttccctttt ctatttcaaa
    51901 caagggctgt cgtattattg cttatcctta ttttacggca ctaccaaaga atacagaaaa
    51961 acaacgaatg attacttatg atgacacatt atacgaaacg gttgatgcgt cggaagcgaa
    52021 agatgttgaa ggatttgtgg aagatttctt agataagtat gcgaaaaaca atgcaaaaga
    52081 tatgtcatac atgatgaaag aacctgaagg actcaatcaa acagctaaag ttgatcaagt
    52141 agagatgaaa gtattgcgtt ataaagaaac gaaaaaacct tttattgtaa aagcaacggt
    52201 cgtctttcga gatggtgata cgtcactttt acaaaaagaa caaatgacga ttgtcattac
    52261 gaaaaaaaca aatcagtatt atgttgaaaa attaacacat aattggttgg ataggaaagg
    52321 agaagaacgg aaatgacact gaagggaatt gtagatttta tattaacgca aggtggctat
    52381 tttttaataa tggtggttgt ttatctggga ttgcgaaatt ttatgaaatc aaaagtagga
    52441 aatgcattaa tatctatcgc agcaggtgcc gcagtttatt tcttcttaaa tgatccaaca
    52501 cgtgtatttg atgcgtttgg taaaatcatt gaaaaattat ttatggatta ggaggatagt
    52561 gaagtggatt gggaacctta tgattattcg attgtttatg cggaacctta tcgcgtacaa
    52621 cagattacaa gcaattggcg tcttccattt ccagttgcat tagtcaatgc agcaactttt
    52681 ttgttggtcg taatctttga atatttattt ttacgttcat ttattggttg ggtcatttct
    52741 ttttttccac cagcaggagt tgttttttat ataggactcc cctatttatg tgtgttagca
    52801 ctggcaaaac taaaacctga tggaaaaaaa gtgcattttt atttatggga ttggttttgt
    52861 tatatgattc gtatgatgaa tggtgattgt gtgtattata actgggacaa ggtatcaaac
    52921 aatgcattgg aagaattact ttttgtgaat aagtatgttt atttagagga ttagaaaaag
    52981 ggacaatcaa ctggttgttc cttttttatt gggaggtgaa attcattggt gcttgtaaaa
    53041 gaaccagtaa aagcattcaa gaaaaattgt ttgttaaatg gagaggattg tatctgggcc
    53101 tactatcatg tttttccacg aacaatttca aaaaacacac caaaaaagac acaggaaata
    53161 aaagaaatga tggctcgttt actgttgctt ttagcagatt atgaagatgt ggagttggtg
    53221 atgtatccac gaacaatgaa tttacggacg aagtttgaca cattaggaaa gggattttct
    53281 cccgatagtc aagcaattgg tgcgtattat gcaaaagaaa cagtgacatt gttggaacga
    53341 gaaggtcaag tctatcgtcc ttcatttatt ttaggaatca aagtcaaaga aaaagcaacg
    53401 gtggagagtt ttcatgatgt aataaatcta atctggttag atttagcagg aagtttagca
    53461 cgcttatttg gattagaagt agatgatttg gaaactttgt tttctgaaag tgatccttat
    53521 gtaaaagaag tgacacaatt actttatagc ttttctattc gtcccttagt aaaaaaagag
    53581 ctgttgtatc tcaatcgtct gcaatatatt cgaggaatgg ctcacaaagt agaagaacaa
    53641 aataattggg aagtactgaa agacaatcaa ggaacgtatg tacgtatacc gaaggaacat
    53701 aaaggccttc ttcaattaga agatgagtta gggagtagtt atttagcatt gttgcctatt
    53761 ggggaaacac ctgaaaatat gagaaattgt gatctatttc aatttgctca ggatctttct
    53821 ttcccagcag aattgcgaat gaaaattcac tatccacgct tgaaaggaca gggaggtatt
    53881 ggtttaaaac tgaattggct atcggaacgg ttcaaaacag agcataagga attggttcaa
    53941 aatgatgaag aacctagtga acgcttatta actgttcgag gattagtaaa acatttaaag
    54001 caacgagtag ataatgggca acctgtcgtc gattggatcg catgttttat tgtatacggt
    54061 caaacggttg aagaagttcg tcaaaatagt gcaacggtta ttcaacaact tcatacgatt
    54121 atccatatcc accgtgcaaa aaatgatcaa cgagaattgt tctataaatg tttattaggg
    54181 caaccattgg gacggcagtt acattggaag caccgaacaa ctgtagaagc agttggcgaa
    54241 tggttgtttt cagcagtatc agaattaggt atggaatccg gctggtatat tggtcggcaa
    54301 gatacattag gggatggtgg ttctcgagga gaaaaaacac cattagaaga attaatttat
    54361 gcgtcaaatc gttttgtctt tttgaatctt ctagccattg cggaaggaat caaaggtgcg
    54421 ttgtttgatg cgcctcatgt agcagtaact ggaaaaaccg gtaaagggaa gaccttttta
    54481 gttggcttgc tctttttgta tagttcgttt atgaatgtac aaagtttatt tgtggatccc
    54541 aaaggagaaa agcgcaaatg gtttacgaaa ctcattcaag acccgtatta tcagaaacat
    54601 taccctttgt ttgtagaaca tttgcaaaaa atgagttatg tcacgttaga tgctacaaat
    54661 ccccgtaatt atggtgtatt agatccaatt gtttgtttat cccgagtaga tgcgaaacaa
    54721 gtagcccagg atatgattga tgagttaagt ccgttaggta ccaatcacct tttaaaaggg
    54781 gcggtattaa agggaatcca agaagtttta gttcaaaaag aagccggaca acaagttggc
    54841 ttgatgcatg tggtcgacta tttagaacaa atggaacaaa aagaggttcg agaatatggt
    54901 gagtttttac gcttgacggt agaagattcg attttacgtt taggattttc ttatggagaa
    54961 aacccgggat tagactttaa tgccaaaaca acgattttag agatccaaga tttgaaatta
    55021 ccaaatgaca atgtccgacc agaactatat acagatgcgg atcgaaagtc tttgtgtttg
    55081 atgattagtt taggtcgttt ttgtgaaatg tttggaaaac gagatagtag taagaaaaca
    55141 gcgatttatt ttacagaagc atgggtattt aataattcga acgcaggacg tagtattatt
    55201 gcagcaatgg cacgtgtggg gcgttcccag atgaatcaat tagtgttaga tacacaattt
    55261 attggtgact taggttcaga taaagaaaaa gggaattttg gtgtcgtctt tgcttttgat
    55321 gaagacagtg agcgagaaaa gattttgaat catttacgtt tggaagtaaa tgaagcgaac
    55381 ttagctgaaa tgagccacat gattaaagga cagtgttgga tgttagatcc ctatggacgt
    55441 gtaggaaaac tcaatgtaca cagtttgttt gaagaaatga ccgctgcatt acaaacaacg
    55501 gaaaaaacaa gtcactctca agtagaagaa caggatacgt taatcatgca atagaaatga
    55561 ggtgaaacaa tggcttatat aaagcataag gaaaagaaat ggtgcgtttt gtttttttta
    55621 acgattggat tcggactgct ttttgttggt ggattggtgt atgcggcaga aaagcaggtg
    55681 gatcaatcga tctatgagat tgggaaattt catattgata ccttttcttc ttatttatct
    55741 gatagtgaat tttcattagg caaaatgtta tttggggcaa atggcacaga ttttctaaaa
    55801 atgatgacga atatgttctt tagtttaagt aaattgattt ggcaaggcct tgattttgtc
    55861 attgagaagc tctatgaagg agctgcaatg aacgagttaa ttcaccagtt tttcactttt
    55921 tcggatacta tttattcaaa aatatacgat cctattggtg cggggattgt cgtcttgtat
    55981 gtgttatatc tatttactct tcgtatattt agagggaaac attacgcaaa aatggtgttt
    56041 attcgatttc tcttcgttgt cattttttca atggtttggt ttggctatgg tagtaacgta
    56101 cctagtaaag gagaagcttt taccaaaggg ttaaatgatt tgtccgtgga ggtagaaggc
    56161 tttatttttt ctgctacaaa tagtctagat gaattggcga caaaagatgt caatggtgtt
    56221 ttagtgccaa cgacacaaaa ggaagccatt caaaaagtgc gccaacttta ttataaagcg
    56281 gctgttgttg atccgtatat attgttaaat tatgggacaa caaatttaga agaattgtat
    56341 caagcagaga ttgaaccaca agaatttcta gccaaggaag cgtcaagtga agaattatct
    56401 gatattgaga caaaagtgac cactgctgca ggagaagata gagggaaaaa gaaggcttat
    56461 cgtgtctata ttcaaccaac taaagctgtt tataaagcag tcgttggttc tatggtgcca
    56521 ttgcttaatc taagtattgg attaccactt gtagtgattg gcatgattcg ttttatcttt
    56581 caaatgttgg tgttgtgttt attacttgtg attccttttt cattactcgc ttcttttgtt
    56641 ccagtatttg attatatgct catgaatacc ttaaagtcct ttttgggctt gatgtttcaa
    56701 aaatcttttt acagtgtgat tattttggtg gcatttttga tctataacat agtagacgat
    56761 ttaattccaa tgaatactgt ttttggtttt ttaggaaata tgtttgtcaa agcactccta
    56821 tcaattttac tcattgtaaa acggaaagat ttattaaaga aactagggtt aggagctgca
    56881 gatcaaacta taaacaacac gaaaaacata gcaagtcatt ctttgtctca aatgaaacaa
    56941 ctgcctggtc aactatcaac tgctggacaa aatttggcaa tcaaaggtgc aggattagca
    57001 gggaatgtgt atcgagcaac gaatccactc gcttctgcag cgatacatgg atttcaaaag
    57061 tttcaacaaa aacgtacacc acaagaatcg gtaaaaggga ggcaagtggt taatcccttt
    57121 gaactaggtg ggcgttcaaa tcaagccttg ccttatagtg gtcgtcatgt tcgttatcat
    57181 ggggtagagc ctgaaatgtc ttctaccaaa tcgaaaacag agtggaattc tggtctttca
    57241 aatggtttga ttccaaattc gtccatgaat tcagtaagac caaaacaaca ggtacaatca
    57301 ttggcacaat tgagtaagca acaagggaat aatcaaagaa aaccattgtt aaataaaggt
    57361 atcgcttttg ttggtcaatc gattccgttg ccttctggaa aagtacccgt gccacaatta
    57421 acagctgctg gttctctttc acaacagatt cgccaagtga aaaatcaggc gaaaaatggg
    57481 agttatccag caatgcagca gttattgaac caatactctg ttcctaattc gtcggtagcc
    57541 actctatcag gaaatcgttc tgtgcaacaa ccggggcaac gttcgtctga aagtaaaccg
    57601 tcgatttccg caccaaaatc gttacaacct gcattttctt ctttagataa gccaacagag
    57661 caatcggaaa aaatagaaaa ggatcctaaa acactgccga ttttggacgc aaaaccagga
    57721 actgatttta ctttaccaaa aggacgaaca gtccaaaaac cattatcgaa gaaaggatga
    57781 ggaagaaatg aagaaagggt gtctaggttg tcttcttttc tttattcttc ccttttttct
    57841 attgccattc tttttttcag gagaactttc tgagcatgga ggagaagcta caggtctagc
    57901 tgtcgctttt aatggggaat ataccgaagg attaccgatt ttttcagaga ttaaaggacg
    57961 tggtccaatc acagatgaac atgcgcaagc agcagttggt gcagctgtaa agtatcagtt
    58021 attaccgagt gtgattcttt ctcaattagg ctatgaatca gcttatggac agtcacaatc
    58081 tggaagaaac gatacgaact tttttgggat tacctggttt cctggttgtc cctacccaca
    58141 aggttctgca cgtggaattg gtggttctga aggtggtttc tatatgaagt ttccgaactt
    58201 aaaagcttgt tatgactatt atggctttat ggtggcgagc caaagcaact ttaatcaatg
    58261 tgtgggcaat aaagatcctg gaagttgttt attgattcta ggtcgtggag gctatgcggc
    58321 agcaggtatt accgaaggga gtccgtatta tcgaggagca atgagtatca taacgtccta
    58381 taacttaacg gaatatgata cttttgcgat caagcattgg aatagcctac caacaaaaaa
    58441 aacttcgctt attgcaggaa aaagtaaagg caatgcgaag gttttggatc ggatcattgg
    58501 acgatccgta aataatgggg aatgttatgg tggtacggca tggtatgtat ctcaattagg
    58561 tggcccacag ttaatgggtt ctggacacat gtttgcgatg ttaatagggg aagattataa
    58621 ctggacaacc tatggttggc aagtaatcat gaacccaaaa ccaagtgatt tacaagcagg
    58681 agatgtggtg aattggcaag caggtggtgc attgtctcct ggaatctatg gtcacaccgg
    58741 aattataaca gatgtttcat atggcggaca acatttctct agtgtggaac agaatgccga
    58801 gcaaggacgg attatggctc ggtatcaacg cacgtacaat caaacaaaaa ttcgtagtct
    58861 ggttcgtaag gtgaaataag aaaggcagtg actagaaacg tgaaaaaagg gataatttta
    58921 gtatttagtt ggttatgttt gataggtctt actggttgtc attggttttc ttctgaaaaa
    58981 caaaaaacac aattgagaac gacagaagaa acaatcgaag cagtaaaaga aacaagtgga
    59041 actcttcaaa gtactgaaag taacagtcaa attggttcat tagaagacga gacagcgcaa
    59101 caaatgcaaa tagatgaaat gacaaaaaat ttcttaaaag attttgctag aaaatggagt
    59161 aatttcacag atgtttataa acgcaatcaa gctgtgaggg actatatgac agatcgttgt
    59221 attaaagaca atagtattga tgtagacccg catgtacaaa tgaaaacaac tgggaaactt
    59281 taccaggtga cacaagatat agagaatccg gatcaatatt tgttatttgg agaagaaaca
    59341 atgaataagg ttactcaatt tgtattactt caattgaaag tagatcaaga agctcaaaag
    59401 attgatgcga tcaagattta ttatgtacgg agtgcgtatt aaaataaaaa atgaacactc
    59461 atatttatta tacaccgaag aaatagaaaa aaacagactg tttttttaag aaaaactatg
    59521 tcattcttta cttgaaaaaa gttgttatag gacagaaagg ataaaacaaa aacggaaaaa
    59581 atatgtctta tgaataacta taagaactat tctatttttt aggagatgga tacgagatga
    59641 aaaaactaat tggctatagt gtgatgtgta ttggatttgt tggtgtggga ttgtttgtat
    59701 cgactgaatc tgcttctgcg gctgaatggt ataatcctat ctggtataat ccgaacattg
    59761 aatgtcaaaa aggctatccg tatggaccga caaatctgaa aaatggaaaa tgtagagtaa
    59821 attggaatgg cgttgtagat agtgtaatta ctggttcaat ggactctgct ctacatggct
    59881 ttacgggtca ctagaaggag gaagaaaatg tctgaagaga aaaaagaaat cgttgaatca
    59941 ttagtagctt taaaagattc tctttctaag attgaatatg ttgataggca agaaattcaa
    60001 aaactaattg atgatactat tattgaaatc caagatgcgc gttgtgaagg tataaaaatt
    60061 tctgtagctt tatctaaagt aatagaaaaa atgaaccgtt cattagcgtt taatggattg
    60121 aagctagata gacaaactag tctgatatgg gatcatttaa aagacttgta tgacaagtct
    60181 aaaagaagtg aacggactgc tgtatcaatc ttaaaaggcc tttggggaat gaattcat
//
LOCUS       DAZKMB010000004        59690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000004 DAZKMB010000000
VERSION     DAZKMB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.004"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..646
                     /locus_tag="ACG8V3_000188"
     CDS             14..646
                     /locus_tag="ACG8V3_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298762.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(780..905)
                     /locus_tag="ACG8V3_000189"
     CDS             complement(780..905)
                     /locus_tag="ACG8V3_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0298763.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1039..1932)
                     /locus_tag="ACG8V3_000190"
     CDS             complement(1039..1932)
                     /locus_tag="ACG8V3_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0298764.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2114..3040)
                     /locus_tag="ACG8V3_000191"
     CDS             complement(2114..3040)
                     /locus_tag="ACG8V3_000191"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0298765.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3125..3886)
                     /gene="pflA"
                     /locus_tag="ACG8V3_000192"
     CDS             complement(3125..3886)
                     /gene="pflA"
                     /locus_tag="ACG8V3_000192"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0298766.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4130..6361)
                     /gene="pflB"
                     /locus_tag="ACG8V3_000193"
     CDS             complement(4130..6361)
                     /gene="pflB"
                     /locus_tag="ACG8V3_000193"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0298767.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6638..9088)
                     /gene="parC"
                     /locus_tag="ACG8V3_000194"
     CDS             complement(6638..9088)
                     /gene="parC"
                     /locus_tag="ACG8V3_000194"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0298768.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9100..11145)
                     /gene="parE"
                     /locus_tag="ACG8V3_000195"
     CDS             complement(9100..11145)
                     /gene="parE"
                     /locus_tag="ACG8V3_000195"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0298769.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11311..11745)
                     /locus_tag="ACG8V3_000196"
     CDS             complement(11311..11745)
                     /locus_tag="ACG8V3_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0298770.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11872..12510
                     /gene="plsY"
                     /locus_tag="ACG8V3_000197"
     CDS             11872..12510
                     /gene="plsY"
                     /locus_tag="ACG8V3_000197"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0298771.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12642..13517)
                     /locus_tag="ACG8V3_000198"
     CDS             complement(12642..13517)
                     /locus_tag="ACG8V3_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0298772.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13599..14390)
                     /gene="codY"
                     /locus_tag="ACG8V3_000199"
     CDS             complement(13599..14390)
                     /gene="codY"
                     /locus_tag="ACG8V3_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0298773.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14408..15808)
                     /gene="hslU"
                     /locus_tag="ACG8V3_000200"
     CDS             complement(14408..15808)
                     /gene="hslU"
                     /locus_tag="ACG8V3_000200"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0298774.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15822..16370)
                     /gene="hslV"
                     /locus_tag="ACG8V3_000201"
     CDS             complement(15822..16370)
                     /gene="hslV"
                     /locus_tag="ACG8V3_000201"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0298775.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16407..17312)
                     /gene="xerC"
                     /locus_tag="ACG8V3_000202"
     CDS             complement(16407..17312)
                     /gene="xerC"
                     /locus_tag="ACG8V3_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0298776.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17510..18886)
                     /gene="trmFO"
                     /locus_tag="ACG8V3_000203"
     CDS             complement(17510..18886)
                     /gene="trmFO"
                     /locus_tag="ACG8V3_000203"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0298777.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18855..20933)
                     /gene="topA"
                     /locus_tag="ACG8V3_000204"
     CDS             complement(18855..20933)
                     /gene="topA"
                     /locus_tag="ACG8V3_000204"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0298778.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21055..21912)
                     /gene="dprA"
                     /locus_tag="ACG8V3_000205"
     CDS             complement(21055..21912)
                     /gene="dprA"
                     /locus_tag="ACG8V3_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0298779.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21968..22735)
                     /locus_tag="ACG8V3_000206"
     CDS             complement(21968..22735)
                     /locus_tag="ACG8V3_000206"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0298780.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22728..23588)
                     /gene="ylqF"
                     /locus_tag="ACG8V3_000207"
     CDS             complement(22728..23588)
                     /gene="ylqF"
                     /locus_tag="ACG8V3_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0298781.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23848..24246
                     /locus_tag="ACG8V3_000208"
     CDS             23848..24246
                     /locus_tag="ACG8V3_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0298782.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24483..25025
                     /locus_tag="ACG8V3_000209"
     CDS             24483..25025
                     /locus_tag="ACG8V3_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0298783.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25108..25659)
                     /gene="lepB"
                     /locus_tag="ACG8V3_000210"
     CDS             complement(25108..25659)
                     /gene="lepB"
                     /locus_tag="ACG8V3_000210"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0298784.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25811..26038)
                     /locus_tag="ACG8V3_000211"
     CDS             complement(25811..26038)
                     /locus_tag="ACG8V3_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0298785.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26035..26553)
                     /gene="msrA"
                     /locus_tag="ACG8V3_000212"
     CDS             complement(26035..26553)
                     /gene="msrA"
                     /locus_tag="ACG8V3_000212"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0298786.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26573..27286)
                     /locus_tag="ACG8V3_000213"
     CDS             complement(26573..27286)
                     /locus_tag="ACG8V3_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0298787.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27289..28185)
                     /locus_tag="ACG8V3_000214"
     CDS             complement(27289..28185)
                     /locus_tag="ACG8V3_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0298788.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28343..29185)
                     /locus_tag="ACG8V3_000215"
     CDS             complement(28343..29185)
                     /locus_tag="ACG8V3_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0298789.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29317..29970
                     /locus_tag="ACG8V3_000216"
     CDS             29317..29970
                     /locus_tag="ACG8V3_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0298790.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30033..30560)
                     /locus_tag="ACG8V3_000217"
     CDS             complement(30033..30560)
                     /locus_tag="ACG8V3_000217"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0298791.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30580..31527)
                     /locus_tag="ACG8V3_000218"
     CDS             complement(30580..31527)
                     /locus_tag="ACG8V3_000218"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0298792.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31539..33422)
                     /locus_tag="ACG8V3_000219"
     CDS             complement(31539..33422)
                     /locus_tag="ACG8V3_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0298793.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(33585..35663)
                     /locus_tag="ACG8V3_000220"
     CDS             complement(33585..35663)
                     /locus_tag="ACG8V3_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0298794.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35676..35951)
                     /locus_tag="ACG8V3_000221"
     CDS             complement(35676..35951)
                     /locus_tag="ACG8V3_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298795.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36313..36768)
                     /locus_tag="ACG8V3_000222"
     CDS             complement(36313..36768)
                     /locus_tag="ACG8V3_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298796.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36922..38130)
                     /locus_tag="ACG8V3_000223"
     CDS             complement(36922..38130)
                     /locus_tag="ACG8V3_000223"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0298797.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38242..38574)
                     /locus_tag="ACG8V3_000224"
     CDS             complement(38242..38574)
                     /locus_tag="ACG8V3_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0298798.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38722..39597
                     /locus_tag="ACG8V3_000225"
     CDS             38722..39597
                     /locus_tag="ACG8V3_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0298799.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39679..40260)
                     /locus_tag="ACG8V3_000226"
     CDS             complement(39679..40260)
                     /locus_tag="ACG8V3_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0298800.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40266..41525)
                     /locus_tag="ACG8V3_000227"
     CDS             complement(40266..41525)
                     /locus_tag="ACG8V3_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0298801.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41527..42573)
                     /locus_tag="ACG8V3_000228"
     CDS             complement(41527..42573)
                     /locus_tag="ACG8V3_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298802.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42808..43083)
                     /locus_tag="ACG8V3_000229"
     CDS             complement(42808..43083)
                     /locus_tag="ACG8V3_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0298803.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43326..44636)
                     /gene="der"
                     /locus_tag="ACG8V3_000230"
     CDS             complement(43326..44636)
                     /gene="der"
                     /locus_tag="ACG8V3_000230"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0298804.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44818..46047)
                     /gene="rpsA"
                     /locus_tag="ACG8V3_000231"
     CDS             complement(44818..46047)
                     /gene="rpsA"
                     /locus_tag="ACG8V3_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0298805.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46182..46862)
                     /gene="cmk"
                     /locus_tag="ACG8V3_000232"
     CDS             complement(46182..46862)
                     /gene="cmk"
                     /locus_tag="ACG8V3_000232"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0298806.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46911..47546)
                     /locus_tag="ACG8V3_000233"
     CDS             complement(46911..47546)
                     /locus_tag="ACG8V3_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0298807.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47612..49015)
                     /locus_tag="ACG8V3_000234"
     CDS             complement(47612..49015)
                     /locus_tag="ACG8V3_000234"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0298808.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(49002..50033)
                     /locus_tag="ACG8V3_000235"
     CDS             complement(49002..50033)
                     /locus_tag="ACG8V3_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0298809.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50064..50285
                     /locus_tag="ACG8V3_000236"
     CDS             50064..50285
                     /locus_tag="ACG8V3_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0298810.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50450..51187)
                     /locus_tag="ACG8V3_000237"
     CDS             complement(50450..51187)
                     /locus_tag="ACG8V3_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0298811.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51340..52680)
                     /locus_tag="ACG8V3_000238"
     CDS             complement(51340..52680)
                     /locus_tag="ACG8V3_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0298812.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52917..54440)
                     /locus_tag="ACG8V3_000239"
     CDS             complement(52917..54440)
                     /locus_tag="ACG8V3_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0298813.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54818..55429)
                     /locus_tag="ACG8V3_000240"
     CDS             complement(54818..55429)
                     /locus_tag="ACG8V3_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0298814.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(55552..55677)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55759..56475)
                     /locus_tag="ACG8V3_000241"
     CDS             complement(55759..56475)
                     /locus_tag="ACG8V3_000241"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0298815.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(56481..57050)
                     /gene="scpB"
                     /locus_tag="ACG8V3_000242"
     CDS             complement(56481..57050)
                     /gene="scpB"
                     /locus_tag="ACG8V3_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0298816.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(57034..57804)
                     /locus_tag="ACG8V3_000243"
     CDS             complement(57034..57804)
                     /locus_tag="ACG8V3_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0298817.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57801..58172)
                     /locus_tag="ACG8V3_000244"
     CDS             complement(57801..58172)
                     /locus_tag="ACG8V3_000244"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0298818.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(58191..59078)
                     /gene="xerD"
                     /locus_tag="ACG8V3_000245"
     CDS             complement(58191..59078)
                     /gene="xerD"
                     /locus_tag="ACG8V3_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0298819.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(59174..59647)
                     /locus_tag="ACG8V3_000246"
     CDS             complement(59174..59647)
                     /locus_tag="ACG8V3_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0298820.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
BASE COUNT        16279 a        12640 c        10175 g        20596 t
ORIGIN      
        1 atttggagat attatggaaa ttcaacgtag aaccactaat tttttacatt taggctcttt
       61 ttttgcaact ttgacgatct tattttttct atgttattca taccttaaga cctcacatgc
      121 gccttctagt gcattaggct ggttgctttt ggatcattct gatatcacag aagccgaaga
      181 aaatccgttt tttttgattt tgtcttctct ttgcggctta ctgttttctg tttatttcgg
      241 tgcctatttt tttcatcgac atcctggtac gttaaacgaa gcttcaagcg tattgattgc
      301 atttaaaacg ttttcttttt tagcaattct tttttattcg tttaatcaca atacgctttt
      361 cttctttatc atgaatggtt tagtcgtgat tttatccatt tttacttatt ttattttttc
      421 actcattttt agccaattga aatgtccgct cttctttcga ttagttcctg tcacctgttt
      481 tatttacagc ttgttttcct tttttgtcct tgctttaatg ccactgacca acccttcgac
      541 gatccaagtc gcaggaaatc tgttggatat gctcttcatc gtcagtctag gtgtatttat
      601 gtggcaaaga aaaaacaaac aaaataaaaa gcattttgaa gcctgactat tacgacttca
      661 taaatgcttt tttagtttta aacacttcaa tctaaaagaa taacccaaga ttggataaat
      721 tattaatgac tcgccattcg tttcttagtg attctggatt atcaaatgga cttcctaatc
      781 taatcagcta gttcttccca taaaatgcca tacttgactg aatcataata ttgattattt
      841 aaaaatctaa cttttcttat cactacttcc tttgtcattc cacatttctc accatttttt
      901 tgcattcctt tgtttccaga ccatgtagtg aatctaatag gtggtagata ggcaaaatac
      961 tcaaataatt cttttaacca taaaggaaga gaaaggctcc tcctgcaaga gtcagtataa
     1021 tttttgctta ctgctctctt aggttttact cactaaaact gttccatatt ctttgatata
     1081 agaaaatgca tgcggtataa atttcttgct cagctcagaa aagatttgtc gtttaagaaa
     1141 ggctatttcc tccattgtca atgaaggatc caatgttggt aattcaatct caaaattgtt
     1201 tttttcgaca ataggtaatt ctaatgtgaa tgattcttct tcctttatag tattcgtcac
     1261 aagtagagac tgatggaaaa tactaggccg gcgattttta tatttattct tacgtatcct
     1321 gtcaacgata acttctattt gatcaaaaaa gtacgattca tttacgtctc caataagttc
     1381 ggctaattct ttgaatgcct tcgttacagg aagctgattc ttctgtacaa aaaagtaatt
     1441 agccaaagta agcgggaagt gttctataga ggaaagtgtg ataaaacggt acttctcatt
     1501 tcctgcagtt gtcctcagca attctgtctc tcggctgttt cttactccat aaaaatacgc
     1561 ttctttccgt tcatcttcga aacaatacgc ccaaataact tctcccatcg ctaatttttg
     1621 ttcatcaaca gagagtctct tcaattgttc agctatggtt tcagcagccg aagtggcttg
     1681 ttgcaagtaa tctttaggat caaatattgg aaagttcaaa tgatatcttc gttcttccct
     1741 cttgattaat cctgcatcga tcaataaatc aagaaaatgg gaaaatttat tttgcgaaaa
     1801 ttcttttttc aattctctta gaatcgtttc ttgttctcga tgttcatata gataatcggt
     1861 caatgaagaa aaaagcggat catcggtcag tttttgataa aagttttcct tattttttga
     1921 ataatagttc atatatgttc tcttttctat ccgttttatt ttacttaaaa ggcttttacg
     1981 taaaaaggag gaagaacaag agtcataatt gactgttatt ctcctccatt tcttcggcct
     2041 ttagtatgtt ctatgtttct ttttatatca atcgcttatc cattcatttt gaatgcttca
     2101 tactaccatt tgattagcta aatacagccg tcaattgagg tacgacttgt tttttacgtg
     2161 aaacgacacc tttaagcaaa gcacggttgt tggtcaaagt tgtttggaaa gcttcttcta
     2221 ctttttcttt tcctttacca gcaacaagta attctgaatc actgtctaag atgtttgtga
     2281 caactaatac gaacagatcg tagccatttg ctttcatttc atcttccatt gctgcttcta
     2341 gctctgcttg tcgatccatc acttcattaa ggtccactgt attgacttga gcgatacgaa
     2401 cgttggaact gcccattggg aaactttttg catccaaatc aagaagaacc gcagctgatt
     2461 tatcacttag atttgttcct gctttcaaca tttcaagtcc ataagtgttt agatcgactt
     2521 cagcgattgc tgcaagttct tttgcagctg cttcatcttc aggcgtacat gttggtgatt
     2581 tgaacaacaa tgtatctgaa atgatagctg agagcatgat tcctgcgatt tctttaggaa
     2641 ttgttacttg gttttcttta tatagtttta aaataatcgt acttgtacat ccaactggtt
     2701 ctgcacgata gtacaatgga ttagctgttt caaagtttgc aatacgatgg tggtctacta
     2761 cagctaaaat atttacttcg ctgatatctg aaatactttg ttggaattca ttatgatcca
     2821 caagcatgac atctgcattt tctgctttag ctgactctac tactcgtggt gcagatagct
     2881 gaaagtggtc taatgcgtat tgtgtttctt cattaggtgt acctagagca actgcttctg
     2941 tttctttacc taattcattt tgcaagtgtg aaaacgcaat ggctgccccg attgcatctg
     3001 tatctggatt ttggtgacca aaaactaaaa tttttgacat gtcattcgct cctttgtgat
     3061 ttctcctatt taataatagc aaaaaaacgg aaagaagcaa tacttatctt ctttccgatg
     3121 cgacctatcg cgttaaatat tttgtatact cttctgtatg aagcaatcgt ttagcatttt
     3181 cgacacgttc ttttgtaggt ggttcgatcc cttctaatgg atatttcaaa ccaagtgttt
     3241 cccacttgta ttttcccatc gtatgataag gaagaatctc tactctgtct acatttgaca
     3301 aggtttgaat aaagttgtct agtcggatca aatattcatc ataatcactt cgctcaggaa
     3361 ctagtacatg gcgaatccaa accggtttgt tgatttttga caaatattga gccatttcta
     3421 aaatattttc attgtcccaa tgcgttagtt ttttatgctc ttcattatcg atgtgtttga
     3481 tgtcaaataa cagtaaatct gtatacttca tcaattcttg aaaacgtgaa aagaaaggtt
     3541 cttcatacgt aaaaggtttt ccgcatgtat ccaatgtagt atgcatcttc cgctcttttg
     3601 cttttttaaa tagttcgatc aaaaaatcga tctgcaatag aggttctcca ccactaacag
     3661 taataccacc tttatctccc cagtaaggtc tgaagcgttc tgctttatct aaaagttcat
     3721 ctgctgtgta ttctttccct ccgccaatat tccaagtatc tggattgtga caaaattggc
     3781 aacgcatgcg gcatccttgc ataaacacga caaagcgcag tccaggtccg tctactgaac
     3841 cgaaagtttc gatagaatga acatagccga ttgttttttc ttccatttaa gttcacatct
     3901 tttctttgat ttcattttaa gtacaagtta gctgtcgaag cgaagctttc tgaacaaaat
     3961 ttttcgcaat tgccgcttcg ttatgtgtaa aatttacagg gacggaacat acaagtcctg
     4021 cccctgtaaa tcgtttcttt caaataagca tactgattat tcgattttga tgcgatggct
     4081 attcaacgtc ctttcgacgc taagtcgaaa aacttttttc acgaactttt tacattctgt
     4141 cgtgtgaagt tctcatcaag acatctaatt gttgttctct tgtcaagtca cggaatttaa
     4201 ctgcataacc agatacccgg atcgtcaagt tcggatattt cattggattt tccattgcat
     4261 cacgcaataa atcattactg aatacattga cattcaaatg ataagctcct tttgagaagt
     4321 atccatctaa gacattacgc aagttttcga ttcttgtatc ttgttctttt ccaagacctg
     4381 aaggattgat tgtttgggta tttgaaattc catccaatgc acagccgtaa ttcattttag
     4441 ctgttgagtt caggctagct aataacccac tttgttccgc attgtaactt ggatttgctc
     4501 ctggcgcaag tggttttcca gcttcacggc catcaggagt atttcctgta tttttaccgt
     4561 aaacaacatt tgaagtaatc gtcaataatg aagttgttgg ttttgagttg cggtaagttt
     4621 tatgacgttt gatttgtccc atgaagtatt ccaagatcca tgaaactaat tcatctgcac
     4681 ggtcatcatc atttccgtat ttagggaagt cgccatccac ttcaaaatca actgctattc
     4741 ctttttcatt acggattgct tttacttttg catatttgat cgctgacaag ctgtctgccg
     4801 catgagagat cccagcaata cctgtagcaa atgtgcgttg caagtcagaa tccatcaaag
     4861 ccagctgagc tgcttcataa gaatatttat catgcatata atgaatgatg ttcaacgtat
     4921 tgacatatag ttctgctaac caatccatca tattcatgaa gttatgtttc acttcttcaa
     4981 agtctagtat ttctgatgtg attggacgat attctggtcc aacttgggcg ccagtcactt
     5041 catcgactcc gccattgatg gcatataata gtgttttagc caagttagca cgagcgccaa
     5101 agaattgcat gtcttttccg acaactgttc ctgatacaca gcaagcaatc gcacaatcat
     5161 cagaacccca ttgttcacga agctgattat cattttcgaa ttgaatcgaa gaactttcga
     5221 tagcgattct gctagcatat gttctgaatc cttctggaag gtgttcagaa tacaaaacag
     5281 tcaagtttgg ttctggtgaa ggtcccatat ttgttaatgt atgaagcaga cggaaatcat
     5341 tctttgtaac taatgaacga ccatctaatc ccatacctgc aatggataaa gtagcccaga
     5401 tagggaatcc tgagaataat tggttatatt caggtgtacg cgcgaatttg accatacgta
     5461 atttcattac taagtggtcg atcaattctt gtgcttcttt ttctgtgatt actccattat
     5521 ccaaatcacg ttggatatag atatcaagga atgctgagat acgtccgatt gacatcgcag
     5581 caccattttg tgatttgatt gctgctaaat atccgaagta tagccattgg acagcttctt
     5641 ttgcatttgc agctggttta gaaatatcat agccatattt cgcagccatt tctttcattt
     5701 gcgctaatgc acgatattgt tcagtgattt cttcacgtaa acgaatcact tcttcactca
     5761 ttgtatgata accagtcata tcatgatctt ttttcttttg ttccatcaaa tagtcgattc
     5821 catacaatgc cacacgacga tagtcaccga tgatacgtcc gcgaccgtaa gcgtctggaa
     5881 gccctgtaat gattttgttt ttacgagcag cacgcatttc agcagtataa gcgtcaaata
     5941 caccttgatt atgtgttttg cgccagtcag tgaaaatatg tgtcaattca tcgtttggtg
     6001 tatagccatt gctttttagc gcattgttag ccatattgat tccgccgaac ggcatgaatg
     6061 cttgtttcaa aggtttgtct gtctgtagac cgacgatttt ttccaaatct ttatccaaat
     6121 agcctgcatc gtgtgaagta atagttgata cgatttctgt gtccatatca tagacaccgt
     6181 tacgctcgaa ttggatatca tttaactttt gcaattcatc ccataatttt tctgttgctt
     6241 ctgttgggcc ttccaaaaag ctgtcatcac cgcgatattc agtgtagtta ttttgaataa
     6301 agtctctagt gtctacgcct tctttccatt tggtaccttt aaatccattc cattgctcca
     6361 ttgtatggac ctcctaaatt tctgttttat gtaacagttt ttacgtgatt agtataacac
     6421 agcaccctat ctttgcaagg gttttcttgt tttttattta acaattttta tgttttattt
     6481 acatatattt aaatcaacgt tgacatacca acatttaagc attgtgaaca ctttcatctt
     6541 tttttgcaaa cgtataagga tttattaaaa atgaatgaaa aagagacgcc acctgtatta
     6601 atacagatgg cgtctcttca ttaaaatact acaacagtta cgactcgatg aaaatctcag
     6661 gagcatcgat aacttcgatg acttctccac cttttttctc atccatcgca aatgatccat
     6721 tcgatgttcg atcacctata gggaaatcag ttgggtcaat cgttaactgc tgcccttttt
     6781 gattcatcaa ggttatttct tttgatcttc cttccgacat atagataata cggtgcggat
     6841 tttttttcag ctcacgcatg accgtcactc cccgcttcgc acgtgaagtt tggctgattt
     6901 cttgagcaag catgcgttta acagctcctc gctgagtaac gattacgatc ggcgtatcac
     6961 cttctgctaa cacaagcagc ccgttgataa tcctatctcc atctttcaaa ttcatagact
     7021 tcactccagc tgcctttgct ccaacaacgg gaacatctgt taaaggatag cgcaaaccca
     7081 ttccttgatg cgttactaag aaaacatcta agcctggctg atcttctttc agatacacag
     7141 caatcagttc atcttcttcc gcctttagct tcatacagct gagcggacgg ctcttatagg
     7201 tgcgccatgg ttcgaactct gtcattcgtg tttgcttgat caatccattc ttgctgatga
     7261 atacaaatgt tttctctgct tgaatttttt gatatgggaa aacagctaaa atcgattcgt
     7321 ctacagacag attggagatt gtttgagaaa tgtgctcacc agcatctttc catttcaaat
     7381 cgggtagttc atggacaggg cggtagatca catttgcttt atttgtgatc agaagaacat
     7441 ggtccaatgt actcaattca cctgagtata gcaagtagtc gccttctcgc atcccgattt
     7501 cttctggttt ggaagcacta taagaacgaa ggcttgttcg cttcacatag ccttctcgcg
     7561 tcacactgac aattacgtct tcttgtgcca ctaagacttg cgtatcaatt ttgatttctt
     7621 cgatttcgtc ttcgattgtc gttagacgag cggaagaata attttttttc acttctcgca
     7681 attctttttt cataacagag aaaagctctt tgtcatttga aaggatttta ttcaactctg
     7741 tgatttgcgc aatcaagctt tcggattcct tttgaagctc tgtgatatcc gtgttcgtca
     7801 aacggtataa ttgcaatgtg acaattgctt ctgcttgttc ttctgtaaat tgaaagacgt
     7861 cgactaaatt ctttttggcg tcttttttgt ctttactttc acgaatcgtt gcgattactt
     7921 catccagaat cgataaagct ttcatcaatc cttctacgat atgctgtctt ttttgagctt
     7981 tggctaaatc gaattgacta cgttttgtta tgacttgctt tcgatgctcg atataacttc
     8041 ttaaaatgtc tttcagtcca acttgatgcg gagtcatatg gtcgatagcg accatattga
     8101 aattgtaatt gatctgtagc tcagtgtttt tgaacaaata attcaaaatt ccttgagcat
     8161 tggcgtcttt tttcaattcg acgacaattt gcagacctgt tctatcactt tcatctcgta
     8221 cttcagctat tccatcgatt ttcttattca aacggatctc atccattttt ttcaccaagg
     8281 tcgctttatt tacttcatat gggatttctg tgatcacgat ttgctgttta ttacccttca
     8341 ttgcctctat ctttgttttc gaacgaagaa taacttttcc tcgtcctgtt tcataagctt
     8401 ttttgatttc atcttttcct tgcaagatcc ctcctgttgg aaagtcaggt ccaggaatat
     8461 attccatcaa tttttcaagg ctcgcttgtg gatgatcgat catatagatt gtgccatcga
     8521 tcacttcagc taagttatga gtcgggattt ctgttgcata tccagcagat atacctgtcg
     8581 atccattgac gagaaggttt gggtatcgag ctggaaggac tgttggttct ttttctgtat
     8641 catcaaaatt ccacacgaga tcgactgttt ctttctcgat atctgctagc atctctccac
     8701 ttaacttgga taatctggct tcagtataac gcatcgcagc aggtggatca ccgtccatac
     8761 tcccgttatt cccgtgcatt tcgatcagta cttcccgcaa cttccagtct tggctcatgc
     8821 ggaccatcgc ttcataaata ctgatgtcac catgaggatg ataattcccc atgatatttc
     8881 cgacagattt tgctgattta cggaatcctt tgtcatacgt attgccatct ttgttcatgg
     8941 caaataatat ccggcgttgc actggcttca atccatcacg gatatcaggc agtgcacgtt
     9001 cttggataat atatttggaa taacgtccga agcggtcacc catcacttct tctaaagtta
     9061 attcttgtac ttcttgtcga ttttccatca ggtgacctcc tattctactt caaataactg
     9121 attgttttca tttttatctg ctcgttcttc atctaataaa tcattagaaa ctgaaggaga
     9181 gatttcagta tcctcttttt tatctaagat actgccatct tcttcgagcg taaattgcac
     9241 gtgattttcg atccattttc ttcgaggttc tactttgtca cccatcagtg tggttactcg
     9301 acgttcagct tgcgcagcat catcgatacg gacgcggatc aacgtacggg aagtcggatc
     9361 catcgttgtt tcccacaatt gttccgcatt catctctcca agtcctttat atcgctgcag
     9421 catatatcct ttgccgactt ttttaatcat tgctgccaat tcgtcatctg tccacgcata
     9481 ttcaatgact tgcttctttc cttgtccttt ggatacttta tataaaggcg gtaacgcgat
     9541 atatactttc ccagcttcaa tcagcggttt catataacga taaaagaaag taagcagtaa
     9601 tacttgaata tgggcgccgt ctgtatccgc atcggtcatg atgattactt tgtcgtaatt
     9661 acagtcttcg atagaaaact caggaccgac tcctgcacca atcgtataga tcatcgtatt
     9721 gatttcttca tttttcaaga tatcttgcat tttagctttt tcggtattga tgacttttcc
     9781 tcgtaaaggt agaattgctt ggaactttct gtctcttcct tgtttagcag atccgccagc
     9841 agaatcccct tcgactaaat acaattcatt tttcttaggg tttcgggatt gtgcgggtgt
     9901 caattttcct gaaagaagag attcgccttt tttacgcttc ttcccatttc ggctttcttc
     9961 tctggcttta cgtgctgctt ctctcgcttc tctggctttg attgctttac ggaccaacat
    10021 ctggctcatt tcgctatttt cctgcaaata aaagcccatt tgttcactga tgacgttatc
    10081 tactacggta cgagcaactg gcgtaccaag tttttctttt gtttgccctt caaattgcag
    10141 aagattttca ggtacacgga tagaaagtac agcagctaac ccttctctga aatcactacc
    10201 ttccagattc ttgtcccgtt ctttcagcaa accaactttt ctcgcatatt cattataaga
    10261 tttcgtcatc gccgctttca taccagcttc atgggttccc ccatcttttg tccgaacgtt
    10321 attgacaaaa gataaaacat tttctgaata accgtcattg tattgataag cgacctctac
    10381 ttcgatacct tctttttctc ctgaaaaata aacaactggt gtcaatgtat ctttttcttc
    10441 attcagataa tcaacaaatt ctttgattcc ttcttcgtaa tgaaaaattt ccttgactgg
    10501 ttcttctcca cgaagatcag aaagagagat tttaacgcct tttagaagaa aggcagattc
    10561 tcttaaccgt tcggctaaaa tttcatatga aaatttcgtt gtactgaata ttgtatcatc
    10621 tggtaaaaaa tggacagtcg tcccgtttgc tttttttgtt tttcctatct ttttcaaagt
    10681 accgtcaggt ttaccgccgt ttttaaattt ctgctgatac tctacgccat ctctaacgat
    10741 cgttacaatt agccatttcg ataaggcatt taccacactg gctcctacac cgtgcagtcc
    10801 accagaagtt ttgtaccctc cttgaccaaa ctttccgcca gcatgcagca cagtaaaaat
    10861 cacttcaact gttggtattc cggaagcatg cattcctaca ggcatcccac gtccgctatc
    10921 tgcaacagtt atactattgt cttcatggat ggtcacatca atctcactgc cataacctga
    10981 taatgcttcg tcaactgcat tatcgacgat ttcatagact aaatgatgca agcctcggct
    11041 gtcagtggat ccaatgtaca tccctggtct ttttctaact gcctctaatc cttccaatac
    11101 ttggatggac gagtcattgt attcgttgtt tacttttttt gccaaggcaa acgctccttc
    11161 aataatactt tgcggcaaaa ccgcaccacg attatcatac tagaaaaggt cgtgaaaaaa
    11221 aagagggtag acgagaaatc aattttaatt atctcctaac cgctttttcg acattcccct
    11281 ctatcgatca tctcttcttt gtggtcattt ttatttttgg ttttctttca acttaccaag
    11341 ttcaatctta atacaacgat ccattacgat atctgtacgt cctgcttttc ttagcatctc
    11401 agctgcttct ttgttttcaa tccctaattg tgcccagaac actttagcat cagttttcaa
    11461 aaaatcacct gctatttcag gtaaaaattc actccgtcta aatacatcta cgatatcgat
    11521 tggatcatcg atttctgtca aattcgcata gactttctcg cctagaattt cttcaccagc
    11581 caataatgga tttactggta tgattttata tccattgtct tgcaatacct gtgcaaccat
    11641 gtaacttgtt cgttctggtt tattgctcaa gcctacgacg gcaatatttt ttgcttcttc
    11701 caaatagttg aaaatcgttt cttgtgttgg attttttaca gacatttctg ccaccccttg
    11761 cttttctttt tattccatct attatacttg tatctttcat tttttcaatt gttcctgtta
    11821 cctattcatg ctaaaataaa ggatagtttt caacttgaaa gagggtaaaa aatgaaattg
    11881 atcttattac ttattttagc atatctttta ggttctattc cttccggtgt ctggattggc
    11941 aaattgtttt tcaaaaaaga tatccgacaa cacgggagcg gtaatactgg aacgaccaat
    12001 acatttcgtg tattaggcaa aacagccggg attgttgtcc tgcttatgga tattttaaaa
    12061 ggtacattgg ctacttgttt gccgatgatc ttccaccttt ctgggattaa ccctctttgg
    12121 tttggggtat gcgccattct tggccataca tttccgattt ttgctgggtt caaaggagga
    12181 aaagctgttg cgacaagtgc aggaatgctt ttaggattta atcctgtatt ttttgtttat
    12241 tcttgtatca tctttatcgt tagtttattt tgtacaagta tggtgagttt gagcagtatg
    12301 atcagtgcag tattgatcac tttatctacg atcgtccttc cttatgtagc tcctgtgatt
    12361 ttggctaagc ccaattggtt actgacgatc attgcctttt tagtttcttc ctttatcttt
    12421 tatcggcata aagacaatat caagcgtatc agaaaccata ctgaaagtcg aatcccgttt
    12481 ggtttgaata gcagcaagaa aaaatcctga tactttttca ttccaaagag ataaaacggg
    12541 agtgtgacaa atcgatgtca cactccctta ttacttacag gatagctttt ttacacacct
    12601 tggtaaacgg tgttccctgc taacttctcg gtcacaacct cttacttcac tgtgatggaa
    12661 tattttgtct tgaagatttc atgggcacct agttgattga tccctacttt ttccatcaag
    12721 tttccactac tgttcaccgt gtcagcgatt ccgcaccacg gttcgataca aacgaaaggc
    12781 gcttcttttg gataaggaga ccagatccct acataaggca tatctttata gcttaaagtt
    12841 acgctatggg aagatttttc acttcgaatg gcgtacgcat ttagacccct tgtttcaaaa
    12901 accaaggcat cttgctcaaa aagttcacga ttcaaagcca gattcgtatt tgtctgacct
    12961 aacgtctttt gatccatatc gatgaatggg cctttcaatg gtaggcgtaa tcttgatttc
    13021 aatggagaaa atgccagata gaaatcttca aatgacaatc cttcttcaag aggtacattg
    13081 aaagcaggat gtccaccgat agaaaagaac atttcttttt caccggtatt tgccacttga
    13141 taatgaaccg tgatgccgtc tcctcctaat tcatagctga tcactaattc aaaatcaaat
    13201 ggatactttt ctaatgtctc cggcgtactc cttaagatga aactagccat ttcttgtcca
    13261 tgttcaatga cttcaaaatc catatcccga gcaaatccgt gttgccccat ttgatatgtt
    13321 ttcccttgat aaacatactg atcatctttt aaacgtccaa caaaagggaa taaaaccggt
    13381 gcatgtctgc cccaatacgc tggatcacct tgccagatat actctaaacc atgttctttt
    13441 gatttcagac tgatcaattc cgcaccgtgt tcagcgattt cggcgatcag ctggtcattt
    13501 tgaataataa cagtcattgc cattcctcca acttcgactt actttttaaa atgggaaggc
    13561 tggaacaaac atttctatct gccccagtcc ccttacttct aataactgtg ttttgctaac
    13621 tcgtctttaa aacgggaatt caatactttt aaataagttc ccttcatgcc taacgaacga
    13681 gattcaatga ttccagctga ttccaatttt cgtaaagcat tgacgatcac agaacgagtg
    13741 atgccgatac tatcggcaat attagaagca gtcagacgac cttcatctcc atccaatgct
    13801 tcaaagatag cttgtacagc cttcaattcg ctataagata atgtgttgat tgccatttgg
    13861 acagccgttg cactccgtac atctgcttcg atgcttcgag acttctgata aaggatctgc
    13921 ataccgacaa ccgtagctcc atattctgct aagactagat cgtcatctgc aaacgattgt
    13981 tccatacgag ataagataat cgtacccaaa cgttcgccag ctccaaagat cggaacgaca
    14041 gtcgtcagac caaatggata tttctctctt gactctaccg gaaatgcagt aatatcacta
    14101 gtaattggga tattagcttc agttttcatc aactgtgcca ctgtatcagt ataagattca
    14161 ggaaattgtt tctcaacaaa cattgttttg attcgttcat tgttcacatc gtgacgttca
    14221 ttataaccta gtaatactcc gtcacttcct atgatatagg tgttgctgtc aagaatacca
    14281 cctagtgtga cagccatttt atcgtaaggc aaatctgctt gcatgtcaaa agtattcttt
    14341 tgctgcaaaa gtgtgtttat cttgcgggtt ttcgttagca agtctgtcat tgtcgtcctc
    14401 cgatttttta taaaatatag cgacttaaat cttcatttgc cgcaattgag ccaagtttgt
    14461 catccacata tgcttctgtg atcgttattt ctcccatttg catatctggt gcttcaaata
    14521 atagatcttc tagtagtttt tccaaaatgg tatgcagtct gcgtgcgccg atattatctg
    14581 tgtcacggtt gacatcgaaa gcgatttgag cgatccgttc aattgcttct tttgtaaacg
    14641 taactgacac attttctgta ccgatcaacg cgacgtattg ttttactaaa gcattatttg
    14701 gttcggtcag aatcttcaca aaatcttcag ctgtcaaatc atctaattct acccgtatcg
    14761 ggaagcgtcc ttgaagttct ggaattagat cacttggttt agacaaatgg aaagctcctg
    14821 aagcaatgaa taggacgtga tctgtttgga tgatcccgta ttttgtgttt acttgtgaac
    14881 cttcgacaat tggcaatatg tctctttgga cgccttctct ggatacttct ccagattgct
    14941 gtgatttaga agtgatctta tcgatttcat cgataaaaat aattccactt tcttccgcta
    15001 atttgattgc ctcactatgg atatcagcat ccttgactaa ttttgctgat tcttccttga
    15061 tcaataactc taaagcttct ttgactgtta ccgtccgttc cacttttttc ttcggtgtca
    15121 aagcacctag agtttcattt aggtcgatcc ccatttgctc caatccattg ttcatcattg
    15181 gtgcaggttt cttttggtct tcaacttcaa tggttacttc acgactatcc agtaaaccct
    15241 tttgtagttg ttccaagact gtacggcgat tgattcgaat atcttctgtc acttcttctt
    15301 gcggttcagc ggtattttgc tgactagcat taaaaagatt catcatttgt tcaaattgat
    15361 tagtggattg tttctgttct tttttgatac caggtaccaa gatttttacc aaacgttttt
    15421 ctgcattttt tttagcactt gtgtatacac ggctgtactg ctctttttct acgatttgga
    15481 tcgcattttc tactaaatcc cgaaccattg attctacatc tcttccgaca taaccgactt
    15541 cagtaaattt tgttgcttcg actttgacaa atggtgcatt gacgattttt gccaaacgtc
    15601 gtgcgatttc tgttttaccg acccctgtag gtccgatcat cagcatattt tttggtgtaa
    15661 tatcttgttg cattttttca tctaactgca aacggcgata gcggttacgc aaagcaacgg
    15721 caacagattt ttttgctgcg tgctgtccta cgatatattc atctaattca cttacgattt
    15781 gtcttggtgt ttgttttaat tcatacatat ctactcacct cttacagttc ttctacgata
    15841 atattatgat tagtaaagat acagatatct gcggcaatat ttaacgcatt ttctgcaatt
    15901 tctttcgcac tcatgtcgtt ttgagcatag tttttcattg ctcgggctgc agccaacgca
    15961 aaattccctc ctgatccgat agctaagata ccatcatctg gtgtgataac ttctcctgta
    16021 cctgaaacaa gaagcatttc tttatcattc attacgatca gcatcgcttc aagtttttgc
    16081 atagattgct gtgtccgcca ttcttgagct aattcgacag ctgcgcgttg tagattcccg
    16141 ttatattcgt ttagtttgcc ttcaaatttt tcttctaaag taaacgcatc tgcaacactt
    16201 ccggcgaaac ctacaacaac ttctccattg tagatacgtc gtactttctt tgcagtgcct
    16261 ttcatgacaa cagattcacc cattgttact tggccatctc cagccatagc aaatttcccg
    16321 tctttttcga cggcacagat agtggttgaa tgaaatggtg attcgaccat gtttgtttcc
    16381 tcctaaattt gttcttttcc ttatttttac gctcttggat ggaaagaacg atagtttttt
    16441 tgtaaacttt cttttgttac atgggcatag atctgggtag tcgataaatt ggcatgtcct
    16501 agcaattctt ggactgtccg caaatctgcc ccattattta ataaatgagt agcaaatgta
    16561 tggcgcagca tgtgaggatg gatatcacta tccaaactgc tttttttgat catttgattc
    16621 aagacatatt caattccagt aggcgtgatt ggttttccat gatgattcac aaaaacgata
    16681 tcatgctctt cgtgatattt tgtcatcaat acctggcggc cttccctgaa atattcttgc
    16741 agagcatctt gagcaaaaga acctagagga gcatatctct ctttgttccc tttaccatgg
    16801 atcaacatga cctctgaatc aaaatcaatg tctgataccg acaaactgct acactcactt
    16861 aaacggatgc ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa atccaaagga
    16921 ctatctcctg ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc
    16981 agtctcaaat ttttcttttt gagatgaacg taagaaaatg gattttcttt tatcacttct
    17041 tctttgagaa gatactgata aaacgaacgt aggctggcta tcttgcgact gatagaattt
    17101 ctgctatagc taggacgatg atcagctaat gcactcaaat agacacgaac cgtaaaatat
    17161 tccgccttca gcagatcagc gtctccagat tcctccaaaa aatgtttgaa atggagaatg
    17221 tcttcttcat atgcttcttt cgttttttct gaatatcctc tttcgacaat caaataacgc
    17281 aaaaattttt gttcccacgt ggcttctgtc attttctcac cttccttttg tcccagagaa
    17341 taatttagca cagcgtttat aaaaagacaa ataaattgtc agaatttaac caatatttct
    17401 aagtttgttc acaattggcg caattcctca cattttgttt gagaaattct taagaaaaac
    17461 gcaagaaaaa gagcgagact atccgtctca ccctcttcat ggagtttcct tactcatttg
    17521 ggattgcagt tttttctaat tctgttttta ttgcctcatt agcttttaaa gctcgttgtg
    17581 caattgcttc atatcttgct tttttgtctc gaatgcgctc tggcagttca ggaaacagac
    17641 catagttggc attcatcggt tggaaatgtt ttccttccgc atgagtaatg tagtaagcca
    17701 tacttccaat agctgtttct tgtggaaact cgactggttc catttctttt gctaagcgtg
    17761 cggcgttgat tccagcaagt agtccgctcg ctgcactttc aacatagcct tctacaccag
    17821 tcatctgacc tgcaaagaac agatcttcgc gtttcgttga ttggtaagtc ggtttcagca
    17881 attctggcga attcataaag ctgtttcggt gcatcacacc atagcgaacg aattcagcat
    17941 tttctaaccc aggaatcatc cggaaaacac gtttttgctc gccccatttc aagtgagtct
    18001 gaaagcccac gatgttgtat aaagaagctg cagcattgtc ttgacgcagc tggataacag
    18061 cataaggacg tttgccagtt ttaggatctt ccaatccaac tggtttcata ggtccgaaaa
    18121 gcatcgtttt gaatcctcgc tgtgccatta cttcaattgg catacatcct tcaaaatatt
    18181 tttctttctc aaaagattta agttctgcca cttcggcatt gattagtgcc tcatggaaag
    18241 ctttgaattc ctcttctgtc atcggacagt tcaagtaagc tgcttctcct ttgtcatagc
    18301 gggacttcaa atatacttta tccatatcga tcgtagcttt gtctacgatt ggtgcagctg
    18361 catcatagaa ataaaaacca tctgaaccat tgaatgcttt gatgctttct gctaatggat
    18421 gagaagtcaa aggtcctgtt gctactacgg tgattccttc cggtatttca gtaatttctt
    18481 cgtttatcac tgttactaat ggatgctctt tgacttttcg ggtaattgtt tcagaaaaag
    18541 aatcgcggtc aacagctaag gcaccaccag ctggcactgc tgtttcatct gccgaagtga
    18601 ttactaccga attcaatcgg cgcatttctt ctttcaataa ccctactgca ttagccaaac
    18661 tgtttcctct taatgaattg gaacatacta gttcagcaaa attacttgtg tgatgcgcct
    18721 cagtcgattt gactggacgc atttcatata aacggacagg gactcctgct tgtgcgatct
    18781 gccaagcagc ttcacttccg gcaagccctg ctccaacaac atttaccatt ttcaatgaaa
    18841 gaacctcttt tctattattt ttggatgtct tcttcgtaat ctccatttac acagactact
    18901 tgtttaccgc ctttgacttt cttctcgacc agatatccgt cacattttgg acaatttctg
    18961 cccactggtt tatcccaaga agtgaattca cattctggga aacgggaaca accatagaat
    19021 agtctgtttt tcttagattt tcgttcaatg acttgtcctt catgacatac tgggcaagtc
    19081 acaccgattt cttttacgat tggtttcgta ttccgacaat caggaaaatt actgcaagca
    19141 taaaacttcc catatttccc taattttatc accatcggat gtccacagac atcacaatcg
    19201 aatcctgctg gttcatcttt gatttgaatt ttttcgattt tttcttccgc attcgtcagt
    19261 tctttttcaa acggtttata gaaacgatca accactttga cccacgcttc cgtgccttct
    19321 tcaattttat ccaaatctcc ttccatttct gctgtgaaat ggatatcgac gatttgaggg
    19381 aagaattcac agattaatga attgacaatt tctcctagtt cagtcggctc aaatcttttt
    19441 tgtgtcaatt ttacatagta gcgtctttgg atcgtatcaa gtgttggtgc ataagtggaa
    19501 ggtcgcccta ccccgttttc ttctagtgca cgaatcaacg tagcctcact aaaacgtgct
    19561 ggtggttgag taaaatgttg ttttggttca atatcaacag attggactac atcgccttct
    19621 tcaagttctg gaagaatatt ttctttctct tctttgccgt catctctacc ttccacatac
    19681 acttgcatga atcctttgaa tttgattttt gaaccgttcg caatgaagat gacaccattt
    19741 tgttctaatg tcactttcat tgtatctaaa atagctggtg tcatctgact agccacaaaa
    19801 cgtgaccaga tcaatgtata aagtttcaat tgatctttat ctaaatattg tttgagttca
    19861 tctggtgtac gaagcacact cgttggacgc acagcttcat gggcatcttg tgcgccttga
    19921 gcatttttga tctttcggtg atcatgtcta gaaaattctt ttccatacgt atcttcaata
    19981 aatgctgcgg cctctgcttt tgctgtatcg gcaatccggg tagaatcagt acgcatgtat
    20041 gtaatcaagc cgactgttcc ttgtttaccc aaagtgattc cttcatataa ttgctgtgct
    20101 accatcatgg tttttcttgt tcgaaagttt aacttacggg cagcttcttg ttgcaggcta
    20161 cttgtagtga aaggattcgc agggtttctt ttgcgctctt tcttttctac ttttttaact
    20221 tcgaagtctg ttccatcaat tctagcagtg acttctttta cactttgtgc atctggtaat
    20281 ttctttttct taccgtcgat tccccagaaa tttgctttga atttcttgcg agctttttta
    20341 aaattaccgt cgatgctcca atattcttct ggaacaaatt ctcggatctc tttttcacgg
    20401 tcaataataa tttttaaagc gacagattgg acacgtcctg cacttaaccc tttcttgacc
    20461 ttacgccaga gaataggact gatcgaataa ccaacgattc tgtctaaggt acgacgagct
    20521 tgctgtgcat ctactaaatc tacatcgatc gttcttggtt ccttaaaagc tgctttgact
    20581 gcgtcttttg tgatttcatt gaagaccacc cgattttttt ctttcagatc caacccaagt
    20641 aggtacgcta aatgccaagc aatcgcttct ccttctctat ccgggtcact tgccaagtaa
    20701 actttttgag ctttttttgc tgcggctttc aggcttttga tgacatcgcc ttttccgcgt
    20761 atagaaatat aatggggttc ataattgttt tcaaaatcga tacccatttt acttttcggt
    20821 aaatctctta tatggccaac actggccatt actttatagt ttcgtccaag atatttctca
    20881 attgtcttgg cttttgcagg tgattctaca atcactaaat atttatacgc cattactgaa
    20941 tggactcctt tccattgtcg tgttcctact attaaaagag gattcataca aatcgtagct
    21001 aatcatatcc attcagaaaa cgtttgtcaa gaaaagcaaa tgaaaagtat ggtgttattc
    21061 tttaaattct gccaagatat cttcacccga aaccgtgcaa actgcaccgt cttggatcaa
    21121 atgatgacat ccggtagaat gttctgttaa aatatttcct ggaacagaaa aaactgttct
    21181 tccaagatcc aatgcttgct gtgctgttat cagagagccg ctttttttct tggcttcaaa
    21241 gacacatgtt cctttagaca aagctgcgat aatccgattt ctcattggaa aacaatgctt
    21301 tttcgggccg gattgaggag gatattcact caaaagaaga tgatcatttg caatcacctc
    21361 ttgcaagtca cgattttcag caggatagac ttgctctatc cctgttccaa tgactgcgat
    21421 tgtccgtcca ccatgagaga ttgctgcttg gtgaccgtat gtatctatcc ctttcgcgca
    21481 gccactgacg atcacatatc cagcttctat cactggtttt agtaaataat aaactgtttt
    21541 tttggctaaa gcagaagtgt gtcttccacc aacaacgctt aacatgttac aagaaagtaa
    21601 agatagatca ccattataaa acagtacaat tggcggatag gtcaagtgaa gcagctgagg
    21661 gggatatttg ggatcaaagc aagtgataaa cgactgtttc gattggattt tgccccagtt
    21721 agcgtgaatt tcctgccaac ttttgcgaaa cagctcccgg tgtttggtta ttttacctat
    21781 cttaatgatg tcttcaaatc cgatttcttt ataaggatac tgcatcatcc agttagcaag
    21841 tgcccatttt ccttgactac tgattccttt tgtataagca agtttcaata aattttcttc
    21901 tatttgatac atcataaaac cgcctttcgt ctctctttct aataagatac gtaaaagcgg
    21961 ctgtttttta ttgtaaatat ttttttacag gtgcaaaact tttacgatgg attggcgtaa
    22021 ttccttgtcg ttcaagtcct tctaagtgtt cctttgttcc ataacctgca ttatgcgaga
    22081 aaccatatcc aggataaagc acgtcatatt ctttcatcaa ttggtctcga tatactttag
    22141 caatgatact tgcagcagca atcgacacag atcgtgcatc ccctttgatt attttttcct
    22201 gctgaatagg caaatccaag gtcatcgcat cgatcaacaa atagcttggt acaatggaca
    22261 atttttctac tgcttggatc atcgctaatt tacttgcttc ataaatattc acatcatcga
    22321 ttgtctgctg atcaacgatc ccgatcccga ttgccagggc tttttcttga acttgttgat
    22381 aaagaagttc tcgctttttc tctgataatt gtttggagtc attcagccct aatatgatct
    22441 catttttcgg caagataact gcagctgcca cgacaggtcc agctaatggt cctcttccta
    22501 cttcatcaat gccggcaatc cattggtgac cttgtgcgta cccttgcttt tcaaagcaaa
    22561 acatttcttc atatttttcc ttaattaatg cttccttttt caatcgtcgt tcaaaagaag
    22621 ccagtgcttg ctgcacgcct ttgcgtggat catttcttaa tgattctagt gtaggatcat
    22681 ctattctctg tatgcttttc aatcgttctt tgatactttg gatggattca gtcatcactt
    22741 ttttgctcca tttcttcgca acgatccagt gtgtatcgtc caagttttcc ttgacgaata
    22801 tcatgcacga ctaattcgct gccacgatca tagtcttctt tatacccacg ttttgctgta
    22861 atatccatca gtaattccgg tgccaatttt tcttcttgct cgattgataa accataacga
    22921 tcgctgattc ttcctgaata atatcgggca aaaaagtcta atccgtaaat cgctaaatca
    22981 tctaaatgga caagctggtc tttgatcgcg cctgtcagcg ctagtttttt gccgatttct
    23041 tcatcttcaa atttgggcca taaaatccct ggtgtgtcta acaattccaa ctcattacct
    23101 aaacgaagcc actgctgacc ttttgttacg ccaggcttat ttcctgtctg tgcaattttc
    23161 ttgcctacta gtcgattcat caacgtagat ttcccaacat ttggaatacc taagaccatt
    23221 gcccgaatag cgcgaggctt gatcccccga gcagcatctc ttgcccgttt ttctttcaat
    23281 gcttctttag ccgcaggtac tagaccttta attcctttac tttcttgtgc cgtgatagct
    23341 aatgcaagat ggccttgttt acgaaaatat tcttgccaca gctttgtctg ttgtggatct
    23401 gccagatccg ctttattcaa taaaacaagg cgaggttttt gctgaatgat ctgatctagc
    23461 attggattac gagaagataa aggtaatcgt gcgtctacca attcaaatac gatgtccaca
    23521 tatttcattt tttcagaaac ttctcgtcgg gcttttgcca tatgtcccgg aaaccattgg
    23581 atggtcattg ttctctccat tcctttcata ccggaatatt ccccggcttt acttgttttt
    23641 ttctttttca atataaattc acttttttat tataaagaaa ttccatctat tagttaagtg
    23701 aaagtgaacc agtcctcatt ctatcacatc ttcacttttt tgatgatgca aatttttatt
    23761 ttggtttcta tatatgaatc taaatttttg acagcgtttc ctaaataaga gatgatagac
    23821 gaacaaaaca gaaaggagac gaaacaaatg aatagtatgg tttttgttaa ttttcctgtt
    23881 gctgatgtca atcgttccgt tgctttttat gaaaagctgg gttttaaaaa aaatgaagaa
    23941 ttttctactg aagaagctgg ttcaatggta tgggatgaca ctttttggat aatgcttctc
    24001 tctcatgatt tttataaaaa atttataaaa gataaagaaa tagctgataa taaaaaaaca
    24061 agtggtgctt tgatttcgtt tagtgttgaa aatgcggatg aagtaaaaaa atttgctcaa
    24121 attgcaaaag aaaatggtgg tactttccac gaagtagata tgggaatgcc tgaagaagaa
    24181 atgttttcat tagaagtcca agatctagac ggaaatacat tagaacccgt atggatgaag
    24241 atgtaagcag ataactaatc tgacgttttt tataacaaaa aaacaaaggc ggatgatacg
    24301 ataagtattc tccgcctcta ttttttttat tcgtacaatc tcttttttac agtttataaa
    24361 agcagtcttc tcaccctttt tttaattgtt agatgccagt gtatgtaaat caccaatgtg
    24421 tcatgacaca ttggtgattt tatggtatcc ttttattatt aactgacaaa ggagagaata
    24481 ttttgaacac tttgattgca aaagacgttt ttagattgcc cgaaatgaat gcaagattgt
    24541 tgtcgacctc tgctctttta ttagcccttg atgtcatctt atacaagata gcgattggtc
    24601 ctgcttattt tcaaatcaca ttcggatttt taagtatggc gcttatgggt tatctatatg
    24661 gaccgatctg ggctggtttg cttgaaatgt tgtcgaacac tttaaatttc acgatatttg
    24721 gttcaggaac gtttcaagct gcttttctta tcacggcttt ccttggtgga tttattaatg
    24781 gcttattttt atatcataaa aagataaacc tgccttccgt catcgctgcc caattattga
    24841 ttatgatccc agtcagcctt tttatcaatt cttggctgat gtctgttttg tttggtgcag
    24901 atttcaaagt aatttttcta ggtagattca tcaggaatat gatccaaatc cctattcaaa
    24961 taatggttgt ttattcattt atcaaaactt tagaggctag aaaagtgttt aaacgagctg
    25021 tttgatttta cgtactaagg tagtaaactg attttggatt aaacaataaa gcgtgcagtc
    25081 tcaacagact gcacgcttta ttgtttctca ctggatccat tttatttcat caagtggata
    25141 atagacaaat cttgctttac ctagaatatc agttttattg atcgtgccaa acgaacgact
    25201 atcttttgaa actcttcgat tatctccaag tacaaaataa ttatcttccg gaagcttgga
    25261 ttgtcccgtt agctcttgta gtgtaaagtc cgtcgtatag gatgcatttg catgatctga
    25321 tttcaggttt ttagttagat aaggttcctt aactactttc ccattgatct tcaattgatc
    25381 attttggtag ctgacatttt ctcctggaag accgattact cgtttgatat aaatcgtgcc
    25441 atctgctaat tgaaaaacaa caatatcaaa tcgtctgatc tccgaaaatt tttccatcac
    25501 aaccatatca ccttgcttca acacgttttc cattgaattg ccttccactg gtaccgggat
    25561 gaataaaaat ccccgcaaca tgaacgcaag gaatacagaa acaagtaagt atttgaatac
    25621 aagccagaag cgattaatat agcgttgttt ttttgtcaat ctctatcaat ccatccctct
    25681 ttttttcatt ctttctttat tataagaaaa atatcaaaaa aatgcgacta gatagctgaa
    25741 ataaaaataa ttctttcaat taggctattg gacaaaaaac ttttttgatc ataaagtaca
    25801 aaactatctc ttatatgtca tgattatttt ctaaatattt ttcccatgct tcgtcaaaga
    25861 tatccatgga cggcaaatag tcgacattca gttccagata attagaaatt tcttcgtagc
    25921 tagagctgtg cttaggaaat tggatgtctt taccggcttc tgtagcgaaa acagataaag
    25981 catcatgtgt gccaccgcct cgcagtgtca ttaaataatg aaaaaagctt cgattcaccc
    26041 ttcgttcctc caattttctt cgataaaggc agcccggcgt gcatggtttc ttttaaaatt
    26101 ttcaggatgc tttttataat actcttgatg gtcttcttct gctagataaa atggttgggc
    26161 tggttcgatt tttgtaacga ttggctgttc gaaacgtcca ctttcagcta actgtttctt
    26221 acttttttca gcaatgatcc gctgttcttc tgtcaaataa aagatcaccg ggcgataatt
    26281 gtcaccgcga tcctcgaatt gtccgaaagc gtcagttgga tcagtttgct gccagtaaag
    26341 ttctactaat tgttcatatg aaatcaatgt tggatcatag ataatctcca ctgcttctgt
    26401 atgtccagtc gtttggcttt ttacttgttc ataagtcgga tctggtacat gccctccagt
    26461 atatcctgac atcactgtat ggataccagg aagcgtgtcg aaaggctgga tcatacacca
    26521 gaagcagcct cctgcaaata ttgctcgttc cattatgatt ccccctctaa tactactcat
    26581 ccgatgattt tttattttct tttgtcttgc tttcagtact gctggtgcta gttttgttgg
    26641 tatttgagtt gtttgaagca tcagaggtat cttttggcaa atatagacta aaccgaatgt
    26701 catcatttac taagtcgatt ttatctgctt taatatacat cccgttttgc attcggaatt
    26761 gatccaacct aagcatgata gtcagatcat ctgtattgac ttcgatccaa tctggtaatt
    26821 tatagtttct cttgatcata ttcatcacat ctttgattgg caaactcaat gttccgatag
    26881 aaagacttct agcttttagt tggacatttc cgttttccat cacatacgga tcaaaatata
    26941 aatagaaatc gataggaaaa cctagtactt cgaaagtacc attgagtagt gcttcatttt
    27001 ccaaataaaa tttatattca atatcactat ctttttgata ctcactaagg aaaaagctga
    27061 tgatttgatt gactttttct ttatttgaat tgatcgttaa gacaggatct ccttgtcttt
    27121 ccgtaagagc tggtatttct gggagttctg gttcccgatt agcaaaaata cggctgccta
    27181 aaaatacggt cgaaccaagt actaccccaa gaaggataag gaaggcgaat ttccagtaat
    27241 ttgttttctt tgttttgttt gaagatacgt cagatcttgt tttcatgttt attgatcatc
    27301 ctccttctct aaccattggt tttgtgtctc gatcagctga tctctcactg catttgccat
    27361 caattgataa cctaaattat ttggatggaa atgatcttct tcatagagca cattattttt
    27421 gatgccattt agattttctg tcgtgctagt tgtttgatct gttccttctt ctgtgacacc
    27481 tacttcctga ttcaatcctt gataaagcaa atcattgatt gggataaagt agcagttcgg
    27541 catttttttt gtcaccgttt ctgttccttt gttccaattg ttaacaattg tctgcatgtc
    27601 agtgatctca ggaaagttca aataaaatgg attgtagatg cctaaaatgt aaataggtgc
    27661 ggttttattg agggatcgaa attcttccaa tagttctgtg acattttcct gatagttttt
    27721 cagtggcttt ttaaaggaat tgaccgttaa cccgaataaa ttattttgga taactttcat
    27781 caaatcatta ccgccaacag tcagtgtgat aaaatcagct gttttgatat tgtttcgaat
    27841 ttcttctttt gtttttactc gctttaagat ctggtcactt cgttcaccat ttacaccata
    27901 gttttctgtt tcaaccgcgg tcaaattata acggtctttt agatcatttg cgacaagggg
    27961 aacgaatcct ccttgcttgg tttgatctcc tactccttca gtcagcgaat ctccgatagc
    28021 tatgtagtga agtttttctt ttttatttgg atcttctctc acagtagtta ctggtttcat
    28081 tagtgggcca gctttcggta tagaagcagc taaacctgca tagatgatga tcgcaattaa
    28141 taatgggaac aaaacgagga tcaaatgtcg tttcgttaat ttcatttttt cacctcttgt
    28201 attcgattgg ttctaatgtt atcaaaaatt ttatcaaaaa ggtagtcttt atgaaaaaag
    28261 aaaacaatta ttttcctaat tttataaaga aaaagagaat aagctgcaag cctttcaggc
    28321 tgagggctta tcctcttttc gactaatcta cataatacat gattgcaaac gcgtctttac
    28381 cagtatgcgt agcgataacc ggattcgtat gcagtacagg gatatgcatg tccgggaata
    28441 attcttgcaa accctctttg aattcttgcg ccaattcttt tgcatccgca tgtgaaatac
    28501 cgatttgtcg cacatttggc aattctttca gttcattttt caaattatta aaccatttat
    28561 cgaacgtttt ctttcctcgc cccttagtta cagggattaa ttcagaatgg gcgaaattca
    28621 tcaccacgcg tatgttcaaa acactagaca gcaaaccagt tgttcggctg attcttccac
    28681 cttttaccaa gttatctagt gtggatattc cgatgtataa ttttgtattg tcttgtactt
    28741 ttttgacttc ttcaaggatt ttctctactg ttttaccttc ttttgctaat tcagctgctc
    28801 gaattacctg aaaagataat ccttggtcag tggtatcact gtcgataacc gttactttcg
    28861 tttttgttag attactggct tgtcttgctg cttctactgt tccactcaag ccttttgtca
    28921 tatggatcga aatcacttca ctgccgtctt ctcctagctc atcatacatc gagatgaatt
    28981 ctccaattgg tggttggctt gttttgggca gattttttgc tttcgccatc atttccataa
    29041 atctttcgcc gtctagttgg tcatcatcgg gatatactgt tccgtcgatc atgatggata
    29101 gcggcatgac acgaatgcct aaagcatcgc gtacattttt ttccatcgta catgaagaat
    29161 ctgttacgat ttttacattt gtcattattt tctctctctc tttctttaac aagatgcctt
    29221 attcatgtta aactacatgt aagacaattt ctatttttag tataacagac aaataatgat
    29281 ttttcattaa ttacacctag taaaggagcc gttttcatgg aaaaaacgaa atttaatcga
    29341 acgtatgaaa tattaaatga agtgtttaat gcagtcaccc atggaattgg gactggattg
    29401 agtatcgcgg gactagtggt tcttttagta aaaggtgccc acttgcattc ctccctccat
    29461 attgtctctt atgctatcta tggatcgatg atgattcttt tatttttaac gtctactttg
    29521 tttcattcat tgatttttac aaaagcaaaa aaggtgttcc aagtattcga tcatagttcg
    29581 atttttcttt tgattgcagg aagttatact cctttttgtt tgctgagtat ccgaggatgg
    29641 ctaggttgga ctttatttgt attaatctgg ttgtttgcga ttcttggcat tatatataaa
    29701 gcgttgacgt tgcataaacg ggagagagta tcaaaagtat ctacgatcat ctatattttt
    29761 atggggtggc tctgtgtcgt ggctgctgtt cctctttatc actctcttgg aatgactggt
    29821 gttattttga tggtagcagg cgggctttct tacacgatag gcgcattctt ttatagcttg
    29881 aattctatcc gctatatgca tgttgtttgg catttgtttg ttatgttagg tgcaggtttt
    29941 atgtacttct ctgttttatt ctttacttaa taaaaaaaat aaagctatgg atgggacagc
    30001 tctaaataac agctagtcta tccatagctt ttttatatcg tcaattcgcc tgtcccgctt
    30061 tttacaggat tctcattacg ctgatacgtt tcaaattgat gcggataacg atttttttca
    30121 tccatttctc cttgactagt attgatcagt gaccaatcag accagtcaac aggtggaaaa
    30181 taagcatctc catcgaatac atgatggatg actgtacggt ataatatcgt acaaaatggc
    30241 agaaattctt gataaacagc tgaccctcca gcgatgaaaa agataccgtc tgtttctttt
    30301 tccagagcaa gcacgtcttc cacactatgc atgacatgca cgccttctgc tttataggaa
    30361 gaatctctcg tcaaaacaat cgtctgacga ttcggcaacg gacggctacc catcccttcg
    30421 aatgtttttc gtcccatcac tagtgtattt gcttctgtca tttgtttgaa gaatttcaga
    30481 tcatttggca aacgccaagg aagacggtct tcttttccaa tcaatccgtt ttcatcctgt
    30541 gcccaaattg cagctaacat tttctcatcc tttctgattc taaacagcga taggagcttt
    30601 gatcgccgga tgtgggtcat atccttctaa ttggatatct tccatctcaa aatcaaatac
    30661 tgactgcttt tcctgattca aaactaaagt cggaaatgcg cgtatttctc ggctcaattg
    30721 ttctttcatt tgttcgatat gattgatata aagatgagca tctcctaatg tgtgaacaaa
    30781 ttcgccaacc gctaaacctg tttcatgtgc aattaggtga gtcagcaacg cataacttgc
    30841 aatgttgaac ggaacgccta gaaacacatc cccactgcgt tgatacaatt ggcagcttag
    30901 ccgtccctct tgaacataaa attgaaacat cgtatgacaa ggaggcaaag ccatgctagg
    30961 aacatcttcc ggattccatg cagaaacgat caatcttcta gaatcaggag tctttttgat
    31021 tgcttcgatc acttgtttga tttgatcgat aaagccaccg tcttttgttt cccagtgacg
    31081 ccattgatag ccgtagatgt gtccaagatc gccgtatgtt tcagcaaagg aatcatcggt
    31141 taggatcttc tcgcaaaaaa gcttgctctg ctttttatat tcttcattga attcaggatc
    31201 tgtcaaacat ctatggccaa aatcagtcat atctggtcca ttatagtctg gacttttgat
    31261 gtagcgttca aatgcccatt catcccaaat atggttttta ttttgtaata gatagcggat
    31321 attcgtatct ccttttaaaa accaaagcaa ttcgcttttg atcaatccaa aaggtactcg
    31381 tttcgttgtt aacaaaggaa accctttttg cagatcaaac cgcatttggt atccgaataa
    31441 gcttaatgtg cctgtgcctg ttctgtcttc tttacgatgc ccttcttcca acaattttct
    31501 tcctaagtca agatacgctt gttccatcct ttttcctcct aatcttctac ccattcactc
    31561 aagtattccc accgtgccat tttttcttca agttgtgctt ctgtttcaga tacgtcgttt
    31621 tgcaattctt gtaatcgagt gaaattatct ccttgatgat tcatctcttc ttggagcaat
    31681 gagatttttt cttcaagttc tgcaatctca tcttcaatcg tttcccattc tttttgttcc
    31741 atgtaagata atttcttctt ttcagcaggt tccttgctct gtatttttgg tttcgctttt
    31801 tcagcaggct gctgggtctc tttttgcttc agtaaataac tggacatgtt tccgtaataa
    31861 agttcttgtt ttccgtttcc ttggaatact aataacttat ctgctacctt gtccaagaaa
    31921 taacggtcat gggaaacagt gatcacagca cctggaaaac ttctaaaata atcttccaaa
    31981 atcgtcaatg ttgctatgtc caaatcattg gtcggttcat ccaataataa aacattaggt
    32041 tgctgaatca gcagtttcaa caaaaataac cggcgttttt ctccgcctga aagtttgcct
    32101 atcagtgtcc catgcataaa tcttggaaat aaaaatcgtt caagtaattc tgctactccg
    32161 atttgactgc catctgctgt ttttgcttct tcagcagctt cttgtaagta agcaatcatc
    32221 cgtttatctg gtgccatttc ttcattttct tgtgtataat acgcaagatg aaccgtttca
    32281 ccgatagaca ttgtaccgct atccagggga attctgccag ctagaatatt aagcaatgtc
    32341 gattttcccg caccattttt accagtaata cctagtcttt cccgagattg aatcaataaa
    32401 tctaaatgtt ctagcagtgt ttggtcattt atacggtaac tgccatcttt gatttccagt
    32461 acttttttcc ctaaacgttg tgtcgctagg ttcagctcta aatcatcttc ttggtttact
    32521 tggtacaagt tctcctttag atcttcgaaa cggttgatcc gtgcttgctg cttggtgcca
    32581 cgtgcctgta cgccagccct catccaagct aattcctgtt tataaagccg cttgcgcttt
    32641 tcttcttgtt ctacttctac acgatcacgt tctgcttttt ctaagacgta agcttcataa
    32701 tttcctttat actcgtataa ttttccaaat gaaagttcga agattcggtt tgctactcta
    32761 tctagaaaat aacgatcgtg ggtaaccatc aatagtgcgc cacggtattg tttcaaatag
    32821 ttttccaacc actcgatcgc ttcataatct aaatggtttg ttggttcgtc tagtaatagt
    32881 aaatcgggtt catcaatcaa cacttgcgcc agactaactc gttttttctg accacctgat
    32941 aaagtactga tttttttatc taattcactg attcctaatt tttgcaaaat aatctttgcg
    33001 ttggtatctg tagtccaagc atcttcttta ttcatttgtt cttctgcttt agcataacgt
    33061 ttttgcacat cagggtcttc tccgttttcc cctagttcga tcaatgcttc ctcatatgct
    33121 cgaacagttt ggatcaaggg gctgtttcct tgaaagactg cttggagaac agttaattca
    33181 tcggaaaatt cagctgcttg agaaagataa ccaatacggt aatcagttgg ataaaacact
    33241 gtttgccgat caccgtcgct gctatcgata ccggcaataa tatttaagag acttgtttta
    33301 cccgtcccgt tcgtcccaat caacccgata cggtcttttt cgtggaccaa aaaagaaatc
    33361 tggtcaaaca aatctttttc accataagtt tttttgaggt cagttacttt taattctttc
    33421 aacatgttcc ctccttatct tggatattgt atcataaaaa tagtgaactt caggaaaatt
    33481 tttttggtat ttgttctttt tgtgttctca aaaaaagaac aggaccgatt cgatcctgtt
    33541 ctgatatgtt ttgtttttta taaaacttga aaatttttac ctttttattt tattggactc
    33601 gttttttttg actttgctcg aaacgcaaat cggaaagaaa agttatcaga tggtttgttc
    33661 atcgtataag attctaacaa attgatattc ctgcgatagc tgttcgcaga aaacgtcgtg
    33721 attttttcag caacttcgta ttcatcaatg atttttcttt caagataata acctcttagc
    33781 atttttttcg taaacagact atcttgctgg atcatccacg aagaaatcaa attccccgag
    33841 ttcattttta ttccttcaag tttgcgttgc tggataaaaa attgaaccat cgtttcatca
    33901 taaatatcag tcaagttatc taaactttca ataatcaact gagtattttt ttgatatgtc
    33961 tctctgattt cttggatatc ttcatcacta ataaatgtct gacgatcagc atatcttctt
    34021 tgccaaaaca ttttcggatg gagaacgatt cgcattaacc tacggacaac aatgaaccaa
    34081 aagccgataa aagacaaaat ctggcttgtg atagaatgtt cgaatcgaga aagaaaacgg
    34141 gagtaaaaac gataaccatt agaagaaagc ttgcccgaac gataactttc atccaatcca
    34201 tcccgttcga tcgacaaaat cagtgcttgt atttcttgta cttcttgctt ttcatcatcg
    34261 gttaaatctt ccaagtacag atcacgtatc cgttcttgat aattttctat cactgcttca
    34321 gtggctaaaa attcttttgt attacgatca atttccttta tttcttttcg caaaacttca
    34381 acgacctctt ctaaaatcat caaagcattc aaatccgtgc ttttcgcttc ctctgtttcc
    34441 gtcagcatag gcaagacaat gatccctatg accaaagtca ctaagatgac acccgccgtc
    34501 agaaacaaaa gcaaagaccg ctggtaaaac atcatattat tgattgaaaa tggcaaaata
    34561 aaaatcgtag ccaaactgac tgttcccttt acaccgccaa atgttaagat caagatctcg
    34621 ttcaactgtt ttttgaactt tttactgcca tctttgaata cataaaaaag actgatagaa
    34681 atgaatctga tcacaaaaag catgacagag attaatacga taatcgctaa taaaagaccg
    34741 tttggatata aaccgtctcc ccatactggc gagaacactt gcgtcaattc gattcctaaa
    34801 aatataaata ctaatgcatt cagtgtaaaa gcaatagttg tccatgtact gtgggacaag
    34861 ctcgacaatt ctgcatcaaa cacagagatt ttcctaaaac cagaagcttg gagaatacct
    34921 gctgccactg cagcaatgat tccagataca tcaaatgcct ctgccaatac ataggctaga
    34981 aaaggaagca aaagttcgat cagcagatac cctgtgacat cttgtgctga tgctttttcg
    35041 atcaaatgaa ttattttttg tttgaaccag acaagcaaaa agccgatcaa agctccccca
    35101 atactagaaa ctactagact catccccgcg tttactgctg aaaaagatcc ggtgatcagc
    35161 gcaccaagag caaactggaa ggcagtaaca ccagatgcat cattcaaaag accttctcct
    35221 tccaaaatat gcatcgcctt tttagggata ttcactcgtc cagaaagaga actgactgct
    35281 accgcatctg tcggacctaa cgctgctcca aaagcaaagc aggcagcaag tgggacactt
    35341 ggcaacagga atgataacgt cgcacctacc ccgacaaggg tcaaaatgac tcctccaaat
    35401 gccaagaaca aaattgtccc gaaatttttg agaatcgaag aaatatctgc tttttctcct
    35461 tctctaaata acaatggtgc aataatcata actaagaaca attcaggttc aaaatcgatt
    35521 gattgtcccc actctgtcag acctaatacc actccaagga aaatttggat cagtggagct
    35581 ggaatcattg gcaaaatgcg gctagctatg ttcgataagg tgatcgttac tgcaaataca
    35641 ataactaact cagcaaattc catcgtccat cccctttatt ttttattttg tgtaagcatt
    35701 tttaaaaatt gttctttttt ctttaaaatt ttggtttcga ctacttcaac ctgtgtttgg
    35761 ttcccctcta attcacactg tacacgtttt gactccaaac gaagcagatc atgtttcaga
    35821 cgttccaact gacgatacat caatatattt cttttacgca acttacaaag tatttgacgg
    35881 cggtcatcca tcgaaagggc gccatagcga tcatgcaaca gttttttttg ttgttcaaaa
    35941 ctctctctca ttttccagat ctcctataca caaacttcca tcattatagc aaagttcttt
    36001 ttggatgaaa aatatttcat tgtttcatcg tttattgcta gttagaaatt tccataaatt
    36061 tttcaacgcc tcattctatt ccaaatagta tcagatatca agtaaaactt aagaaagata
    36121 aaagtttctg acaaaaggat gtttactttc ccattcctgc ctttcttgtt cttttatcac
    36181 aacaaaaaaa tggggctgcg acaaaaatct tgtcacagtc ccttattctg aataaacggt
    36241 gtggaagaga cagcctatca gtagtaataa gacacatctc tttcacagcc tcatttttgc
    36301 gtaaaaatcg atttattcga aaaattcccc gtatacatct tctacacgtt gatctggatt
    36361 gatcacgata tacaagttac ctgttttttc gctgattttt gttgattgat attgattgtc
    36421 taatccttct gttcctttat ctagatatgg gaagtacaca agatcaggtg tgccatttcg
    36481 gtaaaggatt tccattcgct cgatatcttc gtatttcaat gcgcgattaa acatgcccat
    36541 ttccagtccg cctaggttga tatcatgtgt cgcaactgcg tccccttcat ggaagatttc
    36601 gattttaaat ccagcacacg gatggatttc aacaaattca ctgccattga ttcgtccaaa
    36661 acttgttgtg atttgtttga tccaaagatc gccgatatga cggcggttga tcgtccaggt
    36721 ttctccattt ctaaaataaa attttagcgc tgtcatttct ttactcatta aaaagactcc
    36781 tttatgatgt aatttattct tgtactacct cagtttagca caaatttatg taagtggtca
    36841 catcttatgt tttttcttct aatgacgtat ctgcccactt taagtagcca acaaagctga
    36901 gcagatacag tctagtgttt tttaaatcaa tttttctttc gataaaaaat ccaaaacctg
    36961 ttttttatca ttttttattc tttgttctac gacggctttt tcagctaatt gcaaagcttc
    37021 actaacccac ttaccaggtt tttttccagt ttcttctagc aagattttac catctactgc
    37081 taattcgctt aatgacttga taggtaaacg atcataggat tctcctacgg catccatctg
    37141 aggtgtttcg tcaaaataga tcatcaattc ttctacttgc agtgcatgtt ttttgcctaa
    37201 gaagtacaat tcgaatggtt ccaaagctcc agtttctcgt tgtcgtagtc cttggacaag
    37261 tgcttgaacg tctttaatca tttggttaga acacttccac gatttcaaaa aggaacgaat
    37321 ctcattttct tttagatcca gttggctgat caataatgcc catgcttggc tctcttcttt
    37381 caatgggacg gatggtaatt cagcaaaacg taaaagtgct tcgccttctt ctttcaaacc
    37441 gggacagcaa atgtagcact ctgtttcaat aaatgcatgg attcccgcgg cccgttcatt
    37501 tcccaagagc atcttgatga attccacatt tatacgttct acagatattt tacctaatag
    37561 ctcgtggttt tctttgatgg cgtagaaggt ttcttcctct aatttgaacc ctagttgact
    37621 gacaaaacgc aacccgcgca tcattcgtaa ggcatcttca tggaaccgct catgaggatt
    37681 gcctacagct cgcaagatct gttcttctag atcttgcaga ccatcgaaca aatcgatgat
    37741 ttctccatct tctttcaaag caaacgcatt gatcgtaaaa tctcttcttt tcaaatcttc
    37801 tgccaacgaa cgaacgaatt cgacatgatc tggtctacgg tagtcctgat aagttgattc
    37861 cgtccgaaat gtggtgatct catattgttc atcattttct aataccaaca cagttccatg
    37921 ttcgatccca atatctatcg tttttggaaa aagctgtttg atttcttctg gaaatgcgct
    37981 tgttgcgata tccacatcat ggattggctg gttcagcaag atatcacgaa cacttccacc
    38041 tacaaaataa gcttcatatc ctgcattctc taattttttc agaactggaa ttgccttttg
    38101 gtattccaga ggtaattgtt ttaatttcat ttttccaacc tttcctgcgt gtttcttgtc
    38161 ctgagataaa gactcagttg catggtgact gtttgacaag tccgtttcag agtcttcaag
    38221 gccaggacca cttcatcttg tttactcgtc cttacgctca aagcgattat ggtctctttg
    38281 attgaatttt tccatgtttt tttcaaaaac ttcagttaag tcgatattca atgaattagc
    38341 catgatcatc gtaacgaata aaacatcacc aagttcttct gctaccgagt taactggttc
    38401 agaagccttt ttgctttttt ctccataagt gtggttgact tctcttgcca attcacctac
    38461 ttcttcagtt aatcgagcca tctgagctaa tggagagaaa taccccacct tgaattgctg
    38521 gatatattca tccacttcct gctgcatact gtataaagaa cgctttttat ccatcttttt
    38581 ccctccaaaa ctatcttacc aaaactttaa cgcaaatcct agataagagc aattgagaaa
    38641 atcgaatttc catgctacat ttaggaagga atttctaaat ggaaattctg tgaaaataca
    38701 taattgaaag ctggaattca tatggtcaaa cgaaaaacag taaaagaagt tctttttatc
    38761 attataggaa caagtattta tgcatttggt cttgtttact tgaacatcgc taatcattta
    38821 gcagaagggg gagtctcagg gatcaccttg atcttaagag ctttatttgg tattgatcca
    38881 gcttatacga ctttattaat caacatccca ttgattttac taggaggaaa gatcctcggt
    38941 aaacgttcct tggcctatac tgttctaggt acactttctt tgtcattctt tttatggatc
    39001 tggcaaaggg ttccactaga aatcaattta cagcatgatc tactgatcgt ttctctactt
    39061 gcgggtttag ttgcaggtgt aggaagcggg atcgtctacc gcatgggtgg aacaacaggc
    39121 ggcagtgata ttatcgctcg gattttagaa caaaattacg gcatcactat ggggcgttcc
    39181 ctattggcat ttgatatcat cgtcttactt gcttcattaa cctatatcga cttgaaacgt
    39241 atgatgtata cgttgattgt ttcgtatgtt ttcagtcgtg tgattgattc gatcttagat
    39301 ggcggatatt ctgcaaaagg tctacttgtc gtctccaaca aaagcgaaga gattgcacca
    39361 ctactgatga ccggtctgca aagaggtgtg actttccttt ctggagaagg cgcattctct
    39421 ggtaattcaa aaaaaatcat ctatatggtc gttagctcta gtgaattaac tgaagctaag
    39481 aggatcgtcc acgagattga cgaaaaagct ttcctttcaa tcatcaatgt gcatgaagtg
    39541 gaagggcaag gctttactta tttgaagcca cagacaagat tactgaaaaa actataagaa
    39601 aaaagacaaa gataggctat gaggaaagat tccacatcca gctgaccttt gtctttttta
    39661 gtactatact atttttaact aattgttatg ttcacttacc cggttacttt tcttttgtgt
    39721 ctgcaaaagg atctttcggt taacttcatc agataatttc aaaaaagcat ccctatctcc
    39781 atcttctagt gcttgatcga tttgagcata aagcattttt agttttgcct cttcttcttt
    39841 ttcttgaaaa tatcgttcaa ttaattcagt atcttcttca ctgactgaag aatcccaaga
    39901 agaaaatgga ttgtcttcta agatagatag atattctctt gtctgccaag cattttcgaa
    39961 tatacattcc acatataaag gctttttcca attcaaacgg atttcatgaa aaatctggtc
    40021 tgtgtccgta aattcttttc cctgcaaaaa caatttcatg ggttcccctt caacagatag
    40081 atcactgatc tgcagtcctc gatccgcaaa attcgcccct tcaacaaagt ggacattgct
    40141 taaaatcgct tcatgattgg caagataatt caagatccaa aaaacttctc ttcggctaaa
    40201 agatacatga ttgaccatcc acgttaaaaa attcttcttt tcccgtacat caattagcat
    40261 ttttctcacc tctctgttag gtcatctaat aatgacagtg cctccatatc tcctggttca
    40321 agtgttaaat aataagtcaa taattcttgc gctcttttca gctgcccttc ttctcttaag
    40381 aaaaaggcat actctttcaa gaaatctggt tcatgtgata gttcatgata agcctgttca
    40441 tagtgtacag ctgcaacggt aaagtcttct aattcattat aagcatgggc taggttccat
    40501 tctgcataag gattagcagt ttcttccatc tgattgatca cttgtatcac atcttcatat
    40561 ctttcatcat ccaagtaaag attacttaaa gtaagtaatg tttcatcttg tttgtctcct
    40621 acttctaaag ctttcagtaa aagttcttct gctcgctgtc tatcatgcag acggaaagca
    40681 ttttccgagg ccaggtgata aagttcgact tggtaaggat tttctttgat tccttcttct
    40741 aacactgttt gtgcttcttc aatcatttct tcttcttgaa gagcttgtcc tagatataga
    40801 tataacgatt gataatgcgg attaacttct cgaagttctt ccagatagtt gattgcctgc
    40861 tgattttctt tcaattgaag attcgtaaaa gctagctgga acagtcgatc atctgtcctt
    40921 tcttctttca gtgcttcttc taaaggtacg atagctgctt cgaattctcc ttgcatactt
    40981 aaggattgtc ctaaacgttc ctgcatcgag attcctgaaa tttcatttat cccgcttgcc
    41041 aacaatgttt cataaaccac tgctgcctct ttgaaacgat caactgaaaa atacaattca
    41101 gctagcgcaa actggatcaa tggttcatca ggaagcaacc cagctgcttc tttcaatttt
    41161 gcttcactca cttcagggat tccgataact tggtacaaat cagctatcgc caataagctt
    41221 tgcggatagt aatcactttg cttatccact ttttccaggt attcaaaagc taagtcgatc
    41281 tcattatctt cgattgcaat ttctgctaac gggatgttca gtccgtcatt ttctgggtat
    41341 tggttgatca gttgttcaaa aatctgtttt gcttcgatca aaaagccaat agccagcaat
    41401 tcttcgccta gttcagctaa tatatctgct gggtcttctt ttaatgcttt ttctaaaaag
    41461 atttgtgctt gtgacaagtc ttcatctgat aaagcttgca acattttttc gctatttgta
    41521 ttcatttcaa ttccctctta tctcactgac gatttgttga aataccgtca ttgttctcaa
    41581 taattctttt tcgtttggat gttggtatcc gtctattata cctatttttt ctaaaacaag
    41641 tatttttttg cttttttctg ccaaaaaaag aactttttcc aaatactcca tctttgaaat
    41701 ctgttccagc cagtggattg gatagccaag caagtccagc cacacaaaaa agttttttag
    41761 ttggaaatca aaaggatatg cttggagatt ccacacgaaa tgaaacatca ttcctaaaaa
    41821 ttttttcata ctagcagaaa gtagatggcc attttcggtc agataaaaag cttgttcaaa
    41881 cagtttacca taatttgaca ggttttctgc ctctaattga taaaaataga tcatttcttc
    41941 gatcatcccg gcaaacggcc gagtcgttaa ttgcttttta tttggatagt ttttatacag
    42001 attttgcaaa aaacgatgat cacaaatcat tccgcaacag ataaaagtca aaaagtcaat
    42061 ctgctttccg tccacctgcc gctcgctgat tgtttgatca taaacgatgc gttctggcag
    42121 attgatggtt tgcagcacta cacgatttgg tagctgcaaa atcgtccgct gcggtgttaa
    42181 tgctcggctt agagaacgga tcgatacagg gatcatccat agtgatgaat caagggttgt
    42241 atgtttttga aaaaaacctg ccagatccat gatcccttca tttccaaatc cgatgataaa
    42301 catggattga ttttcgggat agcgtttgac gaaatcaagc aggttcttta attctgttaa
    42361 atgattgcat tgagtgttgg cacaaatata ccagtcattg tttgcttgat ttgcaaataa
    42421 cgggttgatc ttatctgcaa acaaatcata atagcgctga ttcgttaaaa ataagattgg
    42481 ctgatcaatg gaaagttctc ttagctgact gactagcgtt tctccgtaaa acactttcgt
    42541 caacgcatct tctacttgat aatcaagttc catacgcatc cctcctcttc ttatttttat
    42601 tttaccatta gtaagcaaat tctagaaaag ataagagcaa agctcacctc ataacttgca
    42661 aataaataaa aattaaataa aatacatgtg ttttctatgt atgacatacg gattcggaat
    42721 ctctgtctct tatttattcc ttttctgcta ggaaataaaa agaaaagcac ttgtagccct
    42781 agagccacaa gcgctttttc aactgtctta tttaacagcg tcttttaatg ctttacctgg
    42841 tttgaatgca ggtactttgc tagctggaat ttcgatttct tcaccagttt gtgggttacg
    42901 acctttgcga gctgcgcgtt cgcgaacttc aaagttacca aaaccgatta attgaacttt
    42961 ttcgccgtta gctaaagcat cttgaatagt tgaaaataca gcatctactg ctgcagttgc
    43021 atctttttta gttaaatcag tagctgaagc aactttttcg attaattctg ctttgtttgc
    43081 catggataga ttcacctcct ccacaagaga tatgatagaa atatcattta gttaaaactt
    43141 tctgagtggt ccagaagttt aaaaagaaat attgattaac ttcaatattc tgttatcacg
    43201 atatcatatg aaccgcttaa tagcaaggtt tttaccaatt ttcacatgct ttttttagtc
    43261 atagaaacgt aacaaacgaa actgtctttt tcttttgaaa aaaaaacgtc gatatggtaa
    43321 aatgcctatt ttctcctacg agggataatt cggatggacg tcccttcaaa agtaaatgct
    43381 tttctgattt gattttctaa gaaacgttca taagaaaaat gcatcaattc ctcttcattg
    43441 acaaaaacga caaatgtagg tggtttgaca gcgacttgtg tagcataaaa aatcttcagg
    43501 cgttttcctt tatctgttgg tgttggattg attgctacag catccataat gatatcattc
    43561 aacacagccg aagagatacg aagattctgg ttcatactta cttgttcgat catttcaggc
    43621 agacgttcca accgttgttt agtcactgca gaaacaaaaa cgattggagc gtaatcaaga
    43681 taccggaatt catcacgaat ctcttgttcg aaatcacgca ttgtattggt ttctttcttt
    43741 acaagatccc atttattcac gacgataata attccacgac cagcttcatg ggcgtaaccc
    43801 gctactcgct tgtcttgttc ccgaatccct tcttcagcat tcagtaccat caaaacgata
    43861 tcagaacggt caatggcacg cattgcacgc atcacgctgt atttttcagt attttcgtat
    43921 acttttccgc gtttacgcat accagctgta tctatcatta agaatttctg accactttca
    43981 gaaacgaagt gggtgtcgat tgcgtctcgg gtcgttcctt caatatccga aacaattaca
    44041 cgttcttctc ctaaaatcgc attgatcaaa gaggatttac caacatttgg gcgaccaatc
    44101 aaactaaact taatcgtgtc gtcgtcctct tcttcagtct cttcagagaa atacttcact
    44161 gcttcatcta aaatatcacc aatccctagt ccatggcttc ctgaaacagg ataaggatcg
    44221 cctagcccta aggaataaaa ttcatagata tcgtttctca tttctggatt atctacttta
    44281 ttgactgcta aaatcactgg tttattactt ctgtataaaa ttttcgcaac catctcatct
    44341 gcgtctgtca cgccttcgcg tccgctgact acacaaatga ttacatctgc ttcttcaatc
    44401 gcgatttctg cttgatgctt gatctgatcc ataaaaggtt catcgccaag atcaattccg
    44461 cctgtgtcga tcacgctgaa ttctcgacct aaccattcac cttttgcata aatacgatca
    44521 cgagttactc caggagtatc ttcgacaatc gagatacgct caccagcgat gcggttaaaa
    44581 atcgtagact taccgacgtt cgggcggcca acgattgcaa ttgttggatt tgccatatta
    44641 tttcctcctc acaagtttcc aatctcactt agtttaacga atgcattgcc aactttcaag
    44701 catagattgt caattaaacg aagtattttt attccttatt gaaatactaa tcgcttttca
    44761 aaagaaaaac tgagacaaaa gcagctgctt tcatctcagt cacagataca aaatcaatta
    44821 tttttcagaa tcatcggttg ctttttcttc tacttcttct ttcaacgcat cacctaaaat
    44881 gtctcccata gtaaatccag tattttcttc tggtaattca taagactcaa cattttttgg
    44941 ttcttcttct gaagctggtt tttcttctaa agctttgata ctcaatgcaa tacgatgttc
    45001 ttctggatgg atctctaata ctttcacttt cacttcgtca ccttcatgaa gcacttcatg
    45061 tggtgtcgca atatgtttat gtgaaatttg agagatatgg actagccctt ctactccagg
    45121 gaacacttca acaaaggcac cgaagcttgt caaacgttta actgttccgt ctaatactgt
    45181 gccaactgga gcttttgtat cgatttctga ccaaggtcca ggctgtgtgt ctttgattga
    45241 caaggaaaca cgttcttgtt caggattgat agacaacact ttgacattta catcatcatt
    45301 gacagccaat acatcacttg gttttgctac atgactatgt gaaatctctg agacatggac
    45361 tagaccgtca attccgccta ggtcgacaaa tgcgccaaaa tcagtaagac gtgcaacacg
    45421 tccactgaga acatctccct catggatagt tgaaagaatt tcttttttct tttcttctct
    45481 ttcgttttcc actaccgctt tatgcgataa gatcaaacga ttttctgatg gttcgatttc
    45541 gatgatttta aagctcaatg ttttgccttt atagtctgaa aagtctgcaa caaaatgatc
    45601 ttccaccatt gatgcaggga caaaaccacg tacaccgacg tcaacgacta aaccgccttt
    45661 gacaacattt gtaacaggtg cttcgatgac cttgccctct tggaaatctc tttcgatatc
    45721 ttcccaaact tttttagcat ctaaacggcg ttttgaaagt agatagctgc cgttttcttt
    45781 atctttgccg atcgtactga taacgactaa atctaataca tcaccgactt ttactacttc
    45841 gctgatatct tctaccggca aagttgataa ttcttttgcc ggaacgactc cttcaactcc
    45901 agccccttcg attccgacaa tcacttgttt gtcttcaatg actaaaactt cgcccttaac
    45961 aacatcgcca acttttactt cttgtacgct tttcattgcg tcttccattg actggttgtt
    46021 ctcttgattt tgatcaaatt ctgtcataac acttgtcctc ctaaccaaca ttctgccgta
    46081 agtacggtac tgtttttagt ttaattgatt attcaaaaaa aataaagcga tttcgttcgt
    46141 tttccttgaa aaaacaattg tttttcattt tttttcttaa tttaaaaaag aagataacct
    46201 tttttcaaaa caacagcttt tattgctgca acgacttctg gaatcgattt gccagtcgta
    46261 tcgatacgaa cagcatcctc tgcttgcttc agcggagaga cgtcccttgt tgaatcataa
    46321 tggtctcttt cctgtatctc tttcgtcagt gtttcaagat cagtcggaat tcctttttct
    46381 tggttctctt tgaaacgtct ttctgcacgc tcacttacgc ttgctactaa aaagattttc
    46441 acttctgcat tcggtaatac agctgtcccg atgtctcttc cgtccatgac aacgccaccg
    46501 cgttccccta tttttcgttg gacagcaact aattcttctc tgacttttcc atgtgcagag
    46561 acttcagaaa cagcttttgt gacatctggt tgacggattt ctaaagtcac atcttttgat
    46621 gctatgaata catgctgtcc atcttcttgt tgcgaaaaag agataggata ctttttgatc
    46681 agcgaaacta gcccttcttc atctgaaaaa gatacattgt tcgtaatagc taaataagtt
    46741 actgcgcgat acatggcacc ggtatctgta taaatataat tcaattcttt tgctaatatc
    46801 ttggcaacgg tacttttccc agatgaagca ggcccgtcga ttgcaatact gatttgtgtc
    46861 atgatataac tcctttttct cagaaaaaaa gccgcaaagc gcggctatta ttatttaatt
    46921 cttaattctt gaccagggta aagcataaag ttgtttggat ctaagccatt caattcaaac
    46981 aattgatcag tggtgatccc tgcacgctca gccacttgtt ttggaccttc gcctgcttga
    47041 acagtcgttg ttgaaccggc tgcagctgat gaagaaggtg ttgtttctgc tgcattgctg
    47101 cttgaatccc ctgcttgata agttgcctca cttgaggaag tcggtgttgt ctctgctgca
    47161 atactgcttt ctgacgttac acctgtatct tgagacacag tagattcttc cgcgattgta
    47221 ctagattcta ctgttgaagt ctcagttgac tcctcttttg ttgtacttgt agaactttct
    47281 actactgatg tcgaagtttc agaagaagtt gctgctgttg cattgttgct tgatccattt
    47341 ctgatccaga aataagcacc tgctggaata gcgatacata agaccagtaa aatcaaaaat
    47401 agcgaaagaa ataatgtatt tcctttcttt tgttgtctct gagaacttct tgatgaaact
    47461 tcatcatctg tatcgtagat tggttgttcc catggttctt gtgtttcgtt attttctgta
    47521 gattgatgtc tatcttttct gctcactgtt ttacctccta aactctatcg tgtgtattat
    47581 accataggaa aaagtgtctt gtcctaaaaa atcaatgttc catgtttttt ttaaataaat
    47641 ttagtaaaat cgttccccaa tgaagttgtt cagtcttaga aaaaagcttc ctctttggca
    47701 gaagttctgc ttttgctcca tcaatatcac aacaattctg cggcttttcg gagagttttt
    47761 cgccaaaata agctaagatg aattttctgc ggcagttttc ttcattaata tagcgaagca
    47821 tctgctgcag gcggaattct ttttcttttt cctgacgttt gagccgttcc acccaatttt
    47881 ctggtttttc agactgctgg atcagctcca tccatttttt ttgcagctca tcaaagggag
    47941 cttgctctgt ggcatattcc aaataaagct caaaactttg tcgctgttct ctagaaagtt
    48001 gattaaagaa ataatggata cgttcatccc cactttgata aaaaagaatt gctgcacttt
    48061 cttcctgatc tctgcctgct cgaccgattt cttgtacata gttttctaga ctgtctggca
    48121 aatcataatg gaccacataa cggatatcag atttatcgat ccccatacca aatgcattcg
    48181 tagcaatgag aaactgcagc tgatttttta caaactgctg ttgaagttgt cttctttgag
    48241 ctgcatcaag accaccgtga tagtatccaa ctgtaaaacg ttccctgaat aaatgataca
    48301 atcgttcgac ttcttttttt gttgcacaat aaatgattgc cgctccttgt gctctttcca
    48361 tgaagtgttc aagttcttgt tctttttgct gcgttttccg aacaaaaagc gagatatttt
    48421 gccgattgac tgattgtctg atctcttttg gtggtcgatc aaatagtaca tgacgtatat
    48481 ctcttgctac taaatctgtt gctgtcgccg ttagtgccag tgtgaccggg tttcctaaaa
    48541 gctttcggat ttttcctaac tgttgatatt ctggacggaa atcaactccc cattgagaca
    48601 cgcaatgagc ctcatctact acatataacg caatattttg ttttttcaga tgctctaaaa
    48661 gagaaggatt agtcaacatt tcgggactca aaaacaaaaa tttatattgc gatagatgac
    48721 ggaggacgta tcttctttca gattgcgaaa gtaaactatt gaaagcagct actcgtttct
    48781 cccctcgttt ttgtaattgc gtcacttgat cttccattag agaaagcagc ggtgacacga
    48841 tgatgaccag accttccatt aaataccccg tcaattgata gcaaaggctt tttcccgttc
    48901 ctgtaggcaa aatagcgaga gtatcttctc catcaagcaa cgcctgaata atttcttctt
    48961 ggccgggacg aaatgaatca aatccaaaat atttttttaa ttcatttatt aacattctgt
    49021 atcgcttcct ttctcaatag atagatttga tacaaacgaa attctccata atctaattgt
    49081 ccatctacat tccattcgct aaagcgccac tctcgcacgg aatccttatt tggttccaaa
    49141 cgttcgattg tttctgcaga gagaatccac tcgtatggaa aatcatcaaa aaacaaggcc
    49201 cactcaataa gatgatctgt tactgttcct ctttttaaat gacggattgc catgatctgg
    49261 tcaattgtct gaccattcat aaacatctgc ttggtgatca tcatgctttg attgaaattt
    49321 tgctgtaata atgaatcgat caaagcgtgc caaagcgaat ctggccgttt ttcgatttgt
    49381 gctaacaaaa ggtggataca tctgctttgt tgcagataaa ccgctgactc atcattgttt
    49441 tttgccagct gataaggaag cagccctgtt cgctcatttc cagataattg gttagctaaa
    49501 aaatctgctg cttcttctgg tatctccgaa aacagctgca ccaaactctc atacgcagta
    49561 tcaatcaaaa tttcacgtgg caattggctt tcactaagcc atttttttag ttggaaagta
    49621 taaaaaggac ttgtctctgc tggaagatag tcttgaatac ccgttgccag attagaaatt
    49681 acttgaacag caaatttaat gagccgccaa ctagtttccc ctgttctgcc atagcggtca
    49741 aaacgcaaac cagtatgttc tatttgatgt tcggacaaca tatcagcccc tgcaggcgtt
    49801 agctttgctt cattttcatt tatttcgatc caaccttgct gcgcaatttt ttgcaaaatt
    49861 tgataaaact tatcctgctt caattctgga aaactgttat ggataaacaa aagttcgtgg
    49921 gtaaatccat aaataagtac tgaagtcgtt cgttttccca caaggagttg gtaaagagaa
    49981 ctaatccgca gcttgtgtcc tgatgaaaat agcgataaaa taaaaacaat catattattc
    50041 tccttaagtt aggatggtta gaaatgtcac tactatgtaa acttgtacct gaacgctgta
    50101 ttgcatgcgg tctttgtcaa atcgaagcgc cagatatttt cgattatgat gaagaaggaa
    50161 tcgttttatt tgcccaagaa ccagaggctc atcaacagtt cgtgatgccg gaagaagaaa
    50221 catatgtaaa aaaagcgtat caaaaatgtc cagttcgagc gattttatta gaaaataaaa
    50281 tataaatata aacgaaactt taaaaacgta cactaagttc tctaaacttg aaaataagcc
    50341 gggatctgtt tctcttgttc cgtgaaatcc cgacttattt tgaaagattc atttgctatc
    50401 tatagttaat tgactagttt tttcacggac cctataggac ccatttatat tatttcaact
    50461 ttttcagcca gttttccaat gtattgacga tcacagtttg ctggtctgcg ttagaaattt
    50521 ctgcctctcc atctcctttt tgctcaccat agctgccaaa acctccatga tttccgccat
    50581 tgattgatac atactctgtt ctttccggta aatactgctt agcttcttga taagcttcct
    50641 gattcaatac tttatctttt gtcgccgtga tcgataaaac aggaacagct gattctgtca
    50701 atgttccttt tttgtcggga taacttgcca tgaagaatac cccttttaaa tgtttgtctg
    50761 aatgattctt attgacataa cgacttgcca ttacgccacc taatgaatgg ccaccgatca
    50821 cataatcgga atttggaaaa tcttctttga ccttatccgc tttgtttccg cccatcactg
    50881 ctagatcaag tggcattttg acaatagcga ctgaaaagcc gttttttgct aaatcttgcg
    50941 cccaaatact ataactttct ggctctacga gagcaccagg ataaaaaata agtaatggag
    51001 actttccatg attctttttc tctccttcaa aaaataagta atcttctttg ttttttcctt
    51061 cttttaccaa cacttgcgct tctgacgatg gttcgtacgt attttgctgt atatagtaat
    51121 aaccggctcc accgagtaat cctaaaatga tgactaagcc aattagtatt cgtctaaata
    51181 ttttcacttt ctcccacctt ttctatctga gtgacccctt tttcttaatc aagtatgatt
    51241 ataccttaaa aaaataagag agaaatgcaa actttccagc atcaaataaa aaaagacgga
    51301 atatccattt gaatatccgc cctttttgta tgtttatttt tatttagcac ataaacgata
    51361 cacagcttct gcataaattt ccattgcttt atacatacta cttaattccc atcgttcatt
    51421 cgcctggtgc ataaagtcag gagtatcagg gaacatcgca ccgaatgcca cacactggtt
    51481 catcgtacga gcaaaagtag cgccgcctga tacaaacggt tcagtcatgt ctccagtaat
    51541 atcgcgatat gtccccaata gatttttcac taactcactg tctagtggca cgtataatga
    51601 atcaaggaag tcaaattctt cataagtcaa cccatatttt tctgctgttt tagttaattt
    51661 agctactaaa tcatctttct tgaatgtaac agggattctc atatctacac cgatttttgt
    51721 ttgttctggt gtgatttcca aacttgcaaa gttcatcgtt agctcgccgg attgttcatc
    51781 ttctgttttg cccacgacag cttcaccagt tgcattttct tgaactaatt ttcctaagaa
    51841 attgattggg ccaaaatcaa atacttctga aagagcaatt gccagacgag aaatagcatt
    51901 cactccttgt gctgcatctt tcgcgtggat acttttacca agaacgacga tgccttcacc
    51961 ttgttcttcg aaatcgaatt cgtgtttttt caatgcttct tttacttcag aaagcttttc
    52021 tccagagtaa ggagctgcat caggaacaac attcaacgct cctccagctt ttacagaaaa
    52081 ctcgttcgtt cctggtcctg tcaagtaagc ttgcaataag cctttttctg cataaattaa
    52141 aggaaattca gcatctggcg caaatccttg cgtaattcct tcttcttttt cgttatattt
    52201 ttctaaacaa cgccataaat tttcttcatc tgtaccaaag ataaaacgaa tacgtgtctt
    52261 gaattccacg ccagcatcaa tcaaagcttt gacggcatac attgccgcaa tagaaggacc
    52321 tttgtcatct tgggatccgc gaccaactaa ccagccgtct tttatagttg gatcgaatgg
    52381 ttttgtttcc caattatttt cgtcccctgc tggaacaacg tccatatgac acaagatacc
    52441 gaataattca tctccagagc caatctctga ataaccgtaa tagccttctg gatctttaaa
    52501 tgttctaaat cctagctttt cactgatttc caatacttta tccaaagctt caatcacttt
    52561 tttaccgaag ggatgtcctt gtcctgaatc tgcttcatct agtacggaag gaattcggat
    52621 caattcgctc aatgattcta atgcttgttc atgatgttgt tctgtcacaa attttttcat
    52681 gatattatcc tcctaatatg ttaaaaaggg tcatgtcaaa ttcctccgac acaacctctt
    52741 cttctcaaat aatgacgttt taagcctgct ccatcagcaa gcagaatgtt cttaacaaat
    52801 gatgaaagaa aaaacactca ctgaatggag ctattgactt atccacgtgc ttatcgatag
    52861 ccgtttatcg gtttcttcat tcttatgata gaccttgtac ctgtatttgt ctaggtttag
    52921 aacattgctg ctacacctag gatcacacag atggctacaa ataatatcgc aattaattta
    52981 ccggtaaatt tccaccacac tgaaacatcg aaacgagcaa ttgctacagc acccattacg
    53041 atagcagatg ttggtgtaat taagtttact aaaccagatg cagattgata agcagtgatt
    53101 actaaatcct taccgacgcc ggcaaattca cccataggtc ccatgattcc cattgttgca
    53161 gcagctaagc cggaagttga aggaatcaag aatgacatag ggatatagaa aataaatgta
    53221 ataatgatga atacacttga agataatgaa cttaatcctt gttcgcccca atgtaagatg
    53281 gtgtctgtga ttaatccatt gttcataacc acttggatac cacgagcaac agctactacg
    53341 attgctacac cgatcaaatc agaagcacca gctaaaaatt cagagacaaa ttcagactct
    53401 ttcatgccat aagtgattgc tactacgatg gacattacta agaataacat agtaatttct
    53461 gggaagtacc agtcacctaa tggaagcatg cttttaccta gaaccgtacc tagtaccgga
    53521 aggtttgtta accagtttgt caatgattca aaaatcgtga aattcgcatt caaggttgtc
    53581 caaggaatca aactgatgat catgatacca aaagtaataa agaacaatac attaacatgt
    53641 ttttgttttt tcgtgattac ttcttgacga ccgattgctt caatatcgaa atgcttcata
    53701 tcttctttac gttggttgta tactaatgat tttgttggat ctttttcgat ctttgatgca
    53761 taacgataca catatacaat agaaatagct aacaaaataa cgaacatcaa gaatcgaaga
    53821 ccaatacctt cacccatcga aatcccaaca gcttgtgaag ctacgcctgt tgcaaaagga
    53881 tttaccgttg aagctagaca gcctacttga gaccctacta atacgattgc tacagctact
    53941 actgtatcaa agcctacagc catcatgact gggatcagta aagcatagaa tggaatcgtt
    54001 tcttctgcca ttccgtaagt agagccgcct aatgcaaata acagcatcaa tactggaata
    54061 agcatttttt cttttccttt gaatttcttc acaactgaag caatcccggc atctagtgcc
    54121 cctgttttgt tgataatacc taaaaatcca ccaacaacca aaatgaagaa agagattggg
    54181 attgcagcag gtgtttcttt agttagctcc cccgctggta cacctagcat cccattgatt
    54241 ggcgccatga aaatatccca cagcccttga ggattttgtt gtactcgttc atatgatcca
    54301 gcgacgatgt tgcctgcatc gtctacacta tagtgcccag ctggtataaa ccaagtaagt
    54361 acagcgatta ctgcaataat aataaataag accgtatacg ctgaaggcat ttgaaatttt
    54421 ttcttttttg tttgttccat cttcttcttc ctttcttttc cgttagagac aacaattttg
    54481 gctctaatgt tattttatca aaaagagtat cagaacaaaa ataataacac gcattttgta
    54541 aaacttttgt tcaaaaagtt cgtttttccc tattccctcc tcttttttca ggtttttaaa
    54601 gctttcttct ttttcattgt agcgtttcct tttttttata aacaaacatt ttcgtctttt
    54661 aaaaggagaa gaatttcaca tttgcaaaga attgattgat ttttatgcat aaatcaaaaa
    54721 aatactcaaa acaaacaaat aaaacgttta cattattgca tgtcttgtat ttttttccaa
    54781 aaaaaaagac caagaaattt cttgatcttt ttgatattta tttgactgct gtatgacgtt
    54841 gttcgaactg atgttgtttt cgacttaacc atggaagaag ttgcgcatgc agcactaaaa
    54901 agacagcact cacgatgaat cctttgatca agttgaaagg aacgatccca atcagtacat
    54961 agtttgctaa agacatgccc agcatctgtg ttgcgttcag ccctaaaaac attaagtaaa
    55021 gaggcgtaat aacgaaataa tttgcgatac tcataaaaat agttagagcg atggttcctg
    55081 taatgacacc taaaaatttg tttcttcctt tatgctgttt ctttacacta aagaaataat
    55141 aaatcgggaa tgtaaagatc gttgttgata agaaactagc agtgtctcct accaaattat
    55201 ccggagaaaa acctgtagtc agcagatgta atactgaacg caagaacgct gttgcaactc
    55261 cggccgctgg accgaataag aacatgctga tcaataccga gatatcacta aaatcgatct
    55321 tcaaaaaact aaaagccggc atgatcggaa aagcaaaata ctgtaaaact aatgcgatcg
    55381 cagcaaacat cgctactgca accattttct gtacacgggt gttcttcatt tgaaatcctc
    55441 ctgttaggac tcctcttcag cgaacttttt gacgtttctc tccaaatgaa aaaactctat
    55501 tttccaggga aaatagagtt agagttattt gatcaagcgt cgtcaaataa gctctatctt
    55561 ctttatccag actatactgt cggtatcgga atttcaccga ttcagctatt tttttgcaaa
    55621 ataggtcgtg gactataacc accggtcggg aatttcaccc tgcccctgaa gacgaacctt
    55681 atataatttt tgtttacata ttcattatac atatctattc aaaaaacgca acatctaaca
    55741 cttataaaaa gtaagtgttc attttgagtg gttcagcaaa ctgctgactt cttttttgtt
    55801 cagttcacgg taatccccag gacgtaggcc ttgtaaagtc agatctccgt atttttcacg
    55861 ttttagtttt tgcacaggaa gaccaactgc ttgaagcata tttttcactt gatgattacg
    55921 tccttcgtgg atcgtcaatt ccacgatact gtggtttgtc ttaggatcca ctgataaaat
    55981 ttggaattta gctggagatg tttttcgtcc ttcgatcttt attccttttg tcaaaggtgc
    56041 aagcatcgat tttgtagcga tcccttttac ctttgcaacg taaactttat ccacttcatg
    56101 ttttggatgt gtcaggcgct gagcaaaatc tccatcattt gtcagtagca agatccccga
    56161 agtatcgtaa tccagtcgac ctacaggata gatacgttct tttaccatcg gcaaaagatc
    56221 cgtaacaact tttcttcctt tgtcatctgc aacagcagaa ataactcctt taggtttgta
    56281 caataaatag taaccgtatt cttcttgata aattgggact tcatccactt ccacagtgtc
    56341 atgtttccca acttttatcc ctaattccct tacaacttgt ccgtttactt ttacacgtcc
    56401 tgcaaggatg aattcttctg cttttcgacg agaagtgatg ccagcgtggg caattacttt
    56461 ctgcaatctc tccattacaa ttattctcct ttattcattc catcaaagaa tagatctggt
    56521 gacacgtctt cttcaagtga aatttccatt tcttgtgtat caggcagttc ttctaatgat
    56581 ttcaaaccaa aataatctaa gaagtagcca gttgttccat ataatatcgg ccgacctggt
    56641 ccttctactc tacctttttc ttcaatcagt ttatgggcag ctagacgctg taccgctcct
    56701 gaaacctgaa ctcctcgaat ctgctcgatt tcagctcttg tgattggctg tttataagcg
    56761 atgattgcta aggtttcgac ggcggcttga gataatgtgt ttgatgtccc ttgcgcatat
    56821 cgcttaagta agggagccag ctctttttta gtcgttaata cataatggct ttccgtttca
    56881 aaaatatgca aagctgagtg cgagttttga gtgtaagatt cttttaattc tttcactaaa
    56941 cttgtgactt ctgcttcgct gagatttaac agtccgctta attcttcatt actgatccct
    57001 tcctcgccaa caataaaaag aagggcttca agttcactta atttcgtcat gttcggtatc
    57061 tcctactgct ggataaagca taattgattc atagttttct ttttgttgcg caatgatctg
    57121 cccttttttc atcaattcta gcaaagccat aaaagtagtg acaacttcac ttttagaagg
    57181 tgactcaaaa aaagaatcga aagtacagcc atgcgcattt ctttttatac gctcttcgat
    57241 aaataccatt cgttcttcga tcgttgtgtc atcgggcgtg atcgtttttt ccactttttg
    57301 agaaaatttc ttacgttcta agattttctg catggcacgg atcaaatcga tttttttgac
    57361 ttgtcctcgc ttcaacggct tttcagcatc cgctaaccca tcgatgttca taggttcttt
    57421 tgtgtaataa agacttcgtt cctctgcttt ttctgtcagt ctttcagctg catatttata
    57481 tttacggtat tctaaaagct gagtgaccaa ttcttctcga ggatcttctt cccataattc
    57541 ttcatcgtct gtgacgaatt cttggacagg aagcaatgtc ttgcttttga ttgccatcaa
    57601 tgtagcagcc atcactaagt attctcctgc aacagctagc tcgagtgttt tcattgtatg
    57661 gatataattc atatattgct ccgtgatcgt agcgatcggt atatcatata tatcgatttc
    57721 catttgctga atcagatgaa gcagcagatc gagaggaccc tcgaatacat ctaatttaat
    57781 attgatctcc gtcaactcat tcactctttc ttctttttct ttgctagcca ttttccaatg
    57841 taaggctctg tgatttcagt tgcacggatg gagacatccg ggtacttttc ctgtaattca
    57901 tctaacattt gaaagcccat attctcaccg cgataagacg gattcaaaga aatgtcatgg
    57961 atgatcagct gatcgttcgt tgcccagtaa ataccaataa tcccttgaat gttggttgaa
    58021 ccttctggcg taaagaaaaa aagttcataa tcgtcttcag attcgtacgt atcaatctcc
    58081 ttcaacaaag aagttttacc tgtcagtttc ttatggaaac tcaatagtcc catagcaatt
    58141 ttacggtttt ctttccggta atgtgttaac atcttctcac cttcctatga ctaagccctt
    58201 ggaaaatgct gcttataaac atccgtcatt cttttcttcg tgatatgtgt ataaatctga
    58261 gtcgtagaga tatccgcgtg acctaacaat tcctgtactg tccgcaaatc agctccattt
    58321 tctagcagat gtgtggcaaa gctatgccgc agcgtatgtg gtgttacttc tttgtaaatc
    58381 ccagcctctc gtactaactg cttcagattt ttccagattc cttgccgaga aagacctttc
    58441 ccatgattat tgacaaatac atgcatttca gatgcgtcct ttacaagtaa tggacgtgct
    58501 tcgtctaagt agcgttccag ccattggatc gcataatcac ctaaaggaat gatccgctct
    58561 ttatctcctt ttccaactgt ctgtaccagt ccaagagaaa gatgaagatc tcccaatttc
    58621 aatccaatca attcactgac gcgcatccca gtagcataca tgacttccaa gattgcacga
    58681 tctcttatcc ctaacgtttt tgtcgtatca ggtgcttcaa tcagcttttc tacttctgtc
    58741 agagtcaatg tacttggtaa tttctgtgct ttcttcggcg tatcgatatg ttgcatcgga
    58801 tcatgatcag tgatacgctc ttgccgtaaa aactgatgga atcttctgag acttgagatc
    58861 atccgagtaa cagttgctgt cgcttttttt tcttcatgca acttctccag aaaacggatg
    58921 actaaaaagc gatcgacatc ctgccatgaa gcaattttct gctcctgtag aaaatgcaag
    58981 tactgttcca aatcacgttc atagctttgt cgtgtattgg cggacaatcc ccgttcaata
    59041 ttcaagtagt ggagataatc gttgatttgc tctttcataa taatccccct catgcctctc
    59101 tatcatatca gataccgctt cattctgata gtctttttcc ataatctgag caaagaaaaa
    59161 gccgtctaga cactcagacg gactggtttc ttttttcttt ttcatagcat tcctgacaga
    59221 ttccatggaa cgttagccga tgatctttta ctaaaaaatg ataacgttct tctactactt
    59281 gctccacttc aagtaaaaga tcctcttcta cttcctcgat cgtgccgcag ttcaagcata
    59341 gtaaatgatg atggaaatgt ttggcgcctt cttttctcaa atcataacgg gctaatccat
    59401 cattaaaact tactttatcg acaattttca attctgtcaa gatttcaagg gttcgataaa
    59461 cagtagcaag tccaatttcc ggactctttt ttttcactaa aaagaagatt tcttctgcag
    59521 ataaatgctc tttctcattt tccaaaagca caagcagagt agcttctctt tgcggtgtca
    59581 gcttaaaccc cgcttcatgt agctgctgtt ttgttttttt catcgccgaa atagcattca
    59641 tgaccattta ctcaacccca acaatttata ataattacaa ataaaaaaag
//
LOCUS       DAZKMB010000005        58881 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000005 DAZKMB010000000
VERSION     DAZKMB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.005"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..545
                     /locus_tag="ACG8V3_000247"
     CDS             27..545
                     /locus_tag="ACG8V3_000247"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0298821.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            673..1356
                     /locus_tag="ACG8V3_000248"
     CDS             673..1356
                     /locus_tag="ACG8V3_000248"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0298822.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1665..1738
                     /locus_tag="ACG8V3_000249"
     tRNA            1665..1738
                     /locus_tag="ACG8V3_000249"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1699..1701,aa:Arg,seq:tct)
     gene            1897..2256
                     /locus_tag="ACG8V3_000250"
     CDS             1897..2256
                     /locus_tag="ACG8V3_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0298823.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2379..4010
                     /locus_tag="ACG8V3_000251"
     CDS             2379..4010
                     /locus_tag="ACG8V3_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0298824.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4072..4713)
                     /locus_tag="ACG8V3_000252"
     CDS             complement(4072..4713)
                     /locus_tag="ACG8V3_000252"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0298825.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4899..4975
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4995..5303
                     /gene="rplU"
                     /locus_tag="ACG8V3_000253"
     CDS             4995..5303
                     /gene="rplU"
                     /locus_tag="ACG8V3_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0298826.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5330..5674
                     /locus_tag="ACG8V3_000254"
     CDS             5330..5674
                     /locus_tag="ACG8V3_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0298827.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5687..5980
                     /gene="rpmA"
                     /locus_tag="ACG8V3_000255"
     CDS             5687..5980
                     /gene="rpmA"
                     /locus_tag="ACG8V3_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0298828.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6414..9221
                     /locus_tag="ACG8V3_000256"
     CDS             6414..9221
                     /locus_tag="ACG8V3_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0298829.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9412..9903
                     /locus_tag="ACG8V3_000257"
     CDS             9412..9903
                     /locus_tag="ACG8V3_000257"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0298830.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10006..10995
                     /locus_tag="ACG8V3_000258"
     CDS             10006..10995
                     /locus_tag="ACG8V3_000258"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0298831.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11028..11831
                     /locus_tag="ACG8V3_000259"
     CDS             11028..11831
                     /locus_tag="ACG8V3_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0298832.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11852..12766
                     /locus_tag="ACG8V3_000260"
     CDS             11852..12766
                     /locus_tag="ACG8V3_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0298833.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12849..13223
                     /locus_tag="ACG8V3_000261"
     CDS             12849..13223
                     /locus_tag="ACG8V3_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0298834.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13362..13607
                     /locus_tag="ACG8V3_000262"
     CDS             13362..13607
                     /locus_tag="ACG8V3_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0298835.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13779..14843
                     /locus_tag="ACG8V3_000263"
     CDS             13779..14843
                     /locus_tag="ACG8V3_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0298836.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15089..15514
                     /locus_tag="ACG8V3_000264"
     CDS             15089..15514
                     /locus_tag="ACG8V3_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0298837.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15507..15956
                     /gene="nusB"
                     /locus_tag="ACG8V3_000265"
     CDS             15507..15956
                     /gene="nusB"
                     /locus_tag="ACG8V3_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0298838.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16053..16907
                     /locus_tag="ACG8V3_000266"
     CDS             16053..16907
                     /locus_tag="ACG8V3_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0298839.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16908..18245
                     /gene="xseA"
                     /locus_tag="ACG8V3_000267"
     CDS             16908..18245
                     /gene="xseA"
                     /locus_tag="ACG8V3_000267"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0298840.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18250..18474
                     /locus_tag="ACG8V3_000268"
     CDS             18250..18474
                     /locus_tag="ACG8V3_000268"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0298841.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18476..19366
                     /locus_tag="ACG8V3_000269"
     CDS             18476..19366
                     /locus_tag="ACG8V3_000269"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0298842.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19375..20193
                     /locus_tag="ACG8V3_000270"
     CDS             19375..20193
                     /locus_tag="ACG8V3_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0298843.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20300..20749
                     /locus_tag="ACG8V3_000271"
     CDS             20300..20749
                     /locus_tag="ACG8V3_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0298844.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20767..22449
                     /gene="recN"
                     /locus_tag="ACG8V3_000272"
     CDS             20767..22449
                     /gene="recN"
                     /locus_tag="ACG8V3_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0298845.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22504..22620)
                     /locus_tag="ACG8V3_000273"
     CDS             complement(22504..22620)
                     /locus_tag="ACG8V3_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0298846.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22693..23649)
                     /locus_tag="ACG8V3_000274"
     CDS             complement(22693..23649)
                     /locus_tag="ACG8V3_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0298847.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23820..24188
                     /locus_tag="ACG8V3_000275"
     CDS             23820..24188
                     /locus_tag="ACG8V3_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0298848.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24358..24789
                     /gene="mraZ"
                     /locus_tag="ACG8V3_000276"
     CDS             24358..24789
                     /gene="mraZ"
                     /locus_tag="ACG8V3_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0298849.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24810..25769
                     /gene="rsmH"
                     /locus_tag="ACG8V3_000277"
     CDS             24810..25769
                     /gene="rsmH"
                     /locus_tag="ACG8V3_000277"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0298850.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25774..26175
                     /gene="ftsL"
                     /locus_tag="ACG8V3_000278"
     CDS             25774..26175
                     /gene="ftsL"
                     /locus_tag="ACG8V3_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0298851.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26176..28368
                     /locus_tag="ACG8V3_000279"
     CDS             26176..28368
                     /locus_tag="ACG8V3_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0298852.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28394..29356
                     /gene="mraY"
                     /locus_tag="ACG8V3_000280"
     CDS             28394..29356
                     /gene="mraY"
                     /locus_tag="ACG8V3_000280"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0298853.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29370..30731
                     /gene="murD"
                     /locus_tag="ACG8V3_000281"
     CDS             29370..30731
                     /gene="murD"
                     /locus_tag="ACG8V3_000281"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0298854.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30757..31845
                     /gene="murG"
                     /locus_tag="ACG8V3_000282"
     CDS             30757..31845
                     /gene="murG"
                     /locus_tag="ACG8V3_000282"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0298855.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31860..33080
                     /locus_tag="ACG8V3_000283"
     CDS             31860..33080
                     /locus_tag="ACG8V3_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0298856.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33236..34561
                     /gene="ftsA"
                     /locus_tag="ACG8V3_000284"
     CDS             33236..34561
                     /gene="ftsA"
                     /locus_tag="ACG8V3_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0298857.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34584..35825
                     /gene="ftsZ"
                     /locus_tag="ACG8V3_000285"
     CDS             34584..35825
                     /gene="ftsZ"
                     /locus_tag="ACG8V3_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0298858.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35842..36519
                     /locus_tag="ACG8V3_000286"
     CDS             35842..36519
                     /locus_tag="ACG8V3_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0298859.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36533..37132
                     /locus_tag="ACG8V3_000287"
     CDS             36533..37132
                     /locus_tag="ACG8V3_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0298860.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37213..37434
                     /locus_tag="ACG8V3_000288"
     CDS             37213..37434
                     /locus_tag="ACG8V3_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0298861.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37483..38265
                     /locus_tag="ACG8V3_000289"
     CDS             37483..38265
                     /locus_tag="ACG8V3_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0298862.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38286..38993
                     /locus_tag="ACG8V3_000290"
     CDS             38286..38993
                     /locus_tag="ACG8V3_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0298863.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39255..42053
                     /gene="ileS"
                     /locus_tag="ACG8V3_000291"
     CDS             39255..42053
                     /gene="ileS"
                     /locus_tag="ACG8V3_000291"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0298864.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42102..43628)
                     /gene="zwf"
                     /locus_tag="ACG8V3_000292"
     CDS             complement(42102..43628)
                     /gene="zwf"
                     /locus_tag="ACG8V3_000292"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0298865.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43643..44290)
                     /locus_tag="ACG8V3_000293"
     CDS             complement(43643..44290)
                     /locus_tag="ACG8V3_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0298866.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44474..44803)
                     /locus_tag="ACG8V3_000294"
     CDS             complement(44474..44803)
                     /locus_tag="ACG8V3_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0298867.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44980..45708
                     /gene="sfsA"
                     /locus_tag="ACG8V3_000295"
     CDS             44980..45708
                     /gene="sfsA"
                     /locus_tag="ACG8V3_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0298868.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45724..46737
                     /locus_tag="ACG8V3_000296"
     CDS             45724..46737
                     /locus_tag="ACG8V3_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0298869.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46737..48014
                     /locus_tag="ACG8V3_000297"
     CDS             46737..48014
                     /locus_tag="ACG8V3_000297"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0298870.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48077..50779
                     /locus_tag="ACG8V3_000298"
     CDS             48077..50779
                     /locus_tag="ACG8V3_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0298871.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50931..51248
                     /locus_tag="ACG8V3_000299"
     CDS             50931..51248
                     /locus_tag="ACG8V3_000299"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0298872.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51278..51598
                     /locus_tag="ACG8V3_000300"
     CDS             51278..51598
                     /locus_tag="ACG8V3_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0298873.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51706..53166
                     /locus_tag="ACG8V3_000301"
     CDS             51706..53166
                     /locus_tag="ACG8V3_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0298874.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53234..53455
                     /locus_tag="ACG8V3_000302"
     CDS             53234..53455
                     /locus_tag="ACG8V3_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298875.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53486..53668
                     /locus_tag="ACG8V3_000303"
     CDS             53486..53668
                     /locus_tag="ACG8V3_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0298876.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53668..54081
                     /locus_tag="ACG8V3_000304"
     CDS             53668..54081
                     /locus_tag="ACG8V3_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0298877.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54204..55385
                     /locus_tag="ACG8V3_000305"
     CDS             54204..55385
                     /locus_tag="ACG8V3_000305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0298878.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55456..55620)
                     /locus_tag="ACG8V3_000306"
     CDS             complement(55456..55620)
                     /locus_tag="ACG8V3_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298879.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55774..55847
                     /locus_tag="ACG8V3_000307"
     tRNA            55774..55847
                     /locus_tag="ACG8V3_000307"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55808..55810,aa:Arg,seq:cct)
     gene            complement(55916..57055)
                     /locus_tag="ACG8V3_000308"
     CDS             complement(55916..57055)
                     /locus_tag="ACG8V3_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0298880.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            57227..57361
                     /locus_tag="ACG8V3_000309"
     CDS             57227..57361
                     /locus_tag="ACG8V3_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298881.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(57354..57989)
                     /locus_tag="ACG8V3_000310"
     CDS             complement(57354..57989)
                     /locus_tag="ACG8V3_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298882.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58102..58737)
                     /locus_tag="ACG8V3_000311"
     CDS             complement(58102..58737)
                     /locus_tag="ACG8V3_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0298883.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58771..>58881)
                     /locus_tag="ACG8V3_000312"
     CDS             complement(58771..>58881)
                     /locus_tag="ACG8V3_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFQ0298884.1"
                     /translation="TQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT        19336 a         9946 c        12496 g        17103 t
ORIGIN      
        1 ttgaataaag aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt
       61 ctataacgtt gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt
      121 ttggtttgac tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa
      181 atctgatata tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat
      241 attatgaaaa ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc
      301 acgaagattt ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa
      361 agagaaatat tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa
      421 aaacggcatt tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa
      481 tcacagcatg gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt
      541 tgtaagaatg ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa
      601 acacgagaaa acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag
      661 aggaggagta gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg
      721 gattttatgc gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa
      781 gctcatgtag cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta
      841 cttggacagc ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca
      901 agtgtcatgg ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat
      961 gtcatgatcg agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact
     1021 ttgcttgaag gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt
     1081 tttatggata gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta
     1141 agaaaaggtg gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca
     1201 gatgaagaga ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt
     1261 gttgtgatgg atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc
     1321 ttaatgacaa aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga
     1381 taatcgattg aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt
     1441 caattaaagg atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct
     1501 gttttcaatt gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt
     1561 tagtagttgg gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag
     1621 aagcaattta attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc
     1681 tggatagagc atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct
     1741 aaaaaaccgt tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg
     1801 tttgttttca aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc
     1861 aaaaaaataa aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta
     1921 gtgactctag ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat
     1981 tctgacacga caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt
     2041 caaacattgt ttaagaacca aggagtgtac aatggactat taggagtggg actactttat
     2101 ggagcttatc tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt
     2161 tttgttgcat tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg
     2221 ctagctctgt tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc
     2281 taacaggaaa ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat
     2341 ggtattttag aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct
     2401 cactaaaaaa agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg
     2461 ggatcacagt agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag
     2521 ctgatgcggc cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat
     2581 caggcggttt ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta
     2641 ttgcggcaac aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt
     2701 tacttgcagc aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa
     2761 ttacggggtt tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat
     2821 ttggtgttca ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag
     2881 gatttccgat aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta
     2941 tctatccaaa aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa
     3001 cagcagttat gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat
     3061 tgatcagtag taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg
     3121 cagttccagc tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt
     3181 cggctggccg aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa
     3241 ttggaaatct gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta
     3301 caagcgtggc catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg
     3361 tcttactgct ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac
     3421 ttgcaggtgt tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc
     3481 tatttactaa taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag
     3541 ttatctttga tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg
     3601 ttgtcaaaag cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc
     3661 tgccagaaac ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt
     3721 ttatgtcttc agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca
     3781 ttttctccat gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat
     3841 ttcaagaaaa agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag
     3901 tagaaaaaca actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg
     3961 ctcatgcgtt gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa
     4021 aagaacagac aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg
     4081 ctgtaaaagt gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt
     4141 gtagaaaata gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc
     4201 gtaggcatcc ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt
     4261 tttggtaacc gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc
     4321 aattaatttt ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac
     4381 ttcggaacga gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt
     4441 gatccgatga gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc
     4501 tcgtgttaag ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa
     4561 aggaacatgc gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg
     4621 atgaagaggt ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga
     4681 agcttcttgt atcagttctt ttgggtattc catttatgct cctccttttt cttttattat
     4741 aagaataaaa gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc
     4801 agaagtattg cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt
     4861 gctgttctgg taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa
     4921 caagtaataa gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg
     4981 aggtgcttta tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag
     5041 tgggacaagc aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg
     5101 acgaagttat cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg
     5161 caactgttga aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat
     5221 acaaacctaa aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg
     5281 ttatcgacgc gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg
     5341 aacatttaag cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga
     5401 tgctggtcct tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac
     5461 tgtcaacggg attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga
     5521 aggcggttat ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc
     5581 tgagattcta ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga
     5641 atatatccaa attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat
     5701 gaatctacaa ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc
     5761 agaatcaaaa cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat
     5821 tctttaccgt caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga
     5881 cgacacttta tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa
     5941 aaaacaagtg tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga
     6001 ggttgtgaca agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga
     6061 ggctacgaag tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg
     6121 atgttttgat aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat
     6181 aattatatgg taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt
     6241 ttcgttcttt taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag
     6301 gtaaatagtt gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa
     6361 tagtctatag aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg
     6421 tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg
     6481 aaattgctga agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag
     6541 tgcgcgaagg aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac
     6601 tcgttacgca cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct
     6661 ctgaagaaaa aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag
     6721 ctaacggcag catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa
     6781 gaggattaaa ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg
     6841 cgatgtatca ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt
     6901 tcaattgtgc tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg
     6961 caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg
     7021 gcggtatgct gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct
     7081 tttactttat ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag
     7141 caaatgtacg gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt
     7201 ttgttcggcg tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg
     7261 agaaagtcga tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga
     7321 tttctttgac agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag
     7381 gacaattgaa gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat
     7441 cacccgaaat ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc
     7501 aattagcaag caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa
     7561 ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata
     7621 atctatatga tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag
     7681 aagcaatcaa tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag
     7741 accatggttt ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca
     7801 tagaagtgac caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc
     7861 aaaactttat ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag
     7921 gagaagaacg gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat
     7981 atgctgttgc gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag
     8041 aaagtgaaga ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg
     8101 gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag
     8161 ttgtccaaca gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg
     8221 atccgaaaac agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg
     8281 gggtcatact cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag
     8341 acactggaat tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg
     8401 tacgaaaggt ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt
     8461 ttcgaggtta cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata
     8521 agaaaaaagc tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag
     8581 aattgatcga acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt
     8641 cgattgtgga agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta
     8701 ctactgggat tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg
     8761 atcaaaatat cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg
     8821 aattcatctc cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata
     8881 acttcatctt tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa
     8941 tctctcagac gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc
     9001 acacagcagg aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg
     9061 aattgacaaa tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt
     9121 tggaagatca gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag
     9181 tccataatca gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac
     9241 ttttatagtg tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc
     9301 gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca
     9361 cagaaatgat aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt
     9421 cgattggcac gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag
     9481 caaacaggtg ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga
     9541 aagtttctac taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag
     9601 aaaatgattc aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc
     9661 accaatcccc aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat
     9721 atcggcggta tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat
     9781 caacaagata ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga
     9841 aaagtacctg ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg
     9901 tagcaagaat ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta
     9961 atatttttga atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc
    10021 ctagcaagtc atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc
    10081 gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga
    10141 gccaaattgg aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt
    10201 gatttatggg gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat
    10261 aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc
    10321 ttttcaatgg aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt
    10381 gtaaaagtaa aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac
    10441 gcacaaccta aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat
    10501 gtattagcac gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa
    10561 gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt
    10621 aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc
    10681 aaaatgatcg aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt
    10741 gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat
    10801 gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga
    10861 caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt
    10921 aaagtaccta atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa
    10981 ttagcaaaag cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt
    11041 caataatttt agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat
    11101 ttcaatttca tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg
    11161 aagctggtat cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg
    11221 gtgccgctgt agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt
    11281 taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg
    11341 ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga
    11401 tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg
    11461 tatgtttaca aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac
    11521 aaactgtgca atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg
    11581 gtgggggaat ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag
    11641 aagtatggcc attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga
    11701 ttgctctagg tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg
    11761 gtggttcatc aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga
    11821 atgactatta atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa
    11881 aaaagatcgt ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga
    11941 acgtatgcaa aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa
    12001 aacaaaagaa gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc
    12061 atacattgct tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg
    12121 cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc
    12181 tggtgttggt gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc
    12241 ttcattagct atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat
    12301 tattcgttgg gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat
    12361 tactgatgac ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg
    12421 gatgttcgta ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt
    12481 atcaactgta aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg
    12541 tgaaggaatc aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa
    12601 ggtaacaaca ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt
    12661 aacattgtta tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg
    12721 tttattcgta gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata
    12781 gctcgggcgt tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga
    12841 gggagaaaat ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt
    12901 ttacgggtct gacagattac gggcaaatca tgataggtaa taatggtttt gagttttacc
    12961 atgcgcgaga tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag
    13021 catcagtcat gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg
    13081 gtacatacac attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg
    13141 tagatgaaga tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc
    13201 gtagtatgtt taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat
    13261 ttttttactt tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat
    13321 cccatatgct aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat
    13381 tgattgttgg agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga
    13441 tgggctgtgt aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt
    13501 tcggcgaatg gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag
    13561 tgattttggt agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat
    13621 agtaaactaa aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa
    13681 taaaaagaag ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat
    13741 aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat
    13801 tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc
    13861 ttcgttactt aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag
    13921 cattttttat tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg
    13981 aaattattaa aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag
    14041 aactgcactc attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag
    14101 aagaaatcat cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag
    14161 tgaaagatga agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat
    14221 atgatcacat cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac
    14281 tagactttta tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta
    14341 gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg
    14401 acttgatcac attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta
    14461 cattcgctat tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg
    14521 ctcaattagc agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag
    14581 ttgctagaga ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac
    14641 atgggatcgg tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat
    14701 ttgtaccagg aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag
    14761 tacgtattga ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac
    14821 caaaagaatt gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt
    14881 tcacagactt tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa
    14941 gctgtaagag ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg
    15001 gcggcagttt tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc
    15061 gtgcttaggc aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc
    15121 aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat
    15181 tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac
    15241 tgaattatta ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg
    15301 cttgaaagta gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat
    15361 ggaaatgcag gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga
    15421 agtcaatatc catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga
    15481 actttttgaa gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg
    15541 aaatggcatt gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg
    15601 cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt
    15661 ttgttcctgt ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg
    15721 ataaagtgat tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg
    15781 atttggtgat cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg
    15841 ctgttgcttt aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta
    15901 aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc
    15961 tagctttttt ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa
    16021 tgaaaacaca aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat
    16081 tagctgaaaa aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta
    16141 tccatcccgg attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa
    16201 gaaataaaga acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg
    16261 agacgatttc tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt
    16321 ttcatggtat attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt
    16381 tagctatccg ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc
    16441 ttgcgggtaa accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag
    16501 cttattctat cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg
    16561 gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta
    16621 aaaccgaaaa tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac
    16681 gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga
    16741 tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta
    16801 ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga
    16861 tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat
    16921 atttaactgt cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt
    16981 tagaacgcgt atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc
    17041 aatatttcag cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat
    17101 tccaaaaatt acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt
    17161 cattatatga agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa
    17221 taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt
    17281 ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc
    17341 caagcggagc ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc
    17401 aattggtttt gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga
    17461 atatccagag agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg
    17521 gttcgattga ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag
    17581 ctaatacgcc ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag
    17641 tagcagatgt tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt
    17701 cagaagagtt gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc
    17761 agatccagcg caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc
    17821 ctgaaagatt gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc
    17881 aagctacaca gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat
    17941 tggaacaagt ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag
    18001 aaaaacgctt agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc
    18061 aagctgttca atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct
    18121 atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc
    18181 tgacgatcca ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag
    18241 aataaaaata tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc
    18301 atgcagttag aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga
    18361 atggaattaa gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa
    18421 atgatgacag aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag
    18481 attaactgac ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt
    18541 agatgagcat acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg
    18601 cgggaaaaga atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat
    18661 tgatgttccc gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat
    18721 ccacgatgat ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca
    18781 taaagtattc ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt
    18841 ccagctaatc agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt
    18901 agctgtttgt gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga
    18961 atcgaaaaag ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt
    19021 gattcgttat gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt
    19081 gctacttcaa cgattagccg aacatttagg tttagctttt cagataagag atgatctact
    19141 cgatgtgatt ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga
    19201 aaaaaatact tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga
    19261 gctgttatcc gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg
    19321 cgggctgatg cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa
    19381 aaagaacgcg tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc
    19441 aaacgttcag tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg
    19501 ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt
    19561 tctcgagggg gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga
    19621 aaaacattgt tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat
    19681 ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga
    19741 caagatccta gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac
    19801 tttaccttag gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg
    19861 atgctgcctc cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa
    19921 ccccaattcg aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag
    19981 acccatcaaa aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg
    20041 ctggaattaa gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac
    20101 ttgaggaaag tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt
    20161 tcaaatgcac acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa
    20221 gtatgcggat tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat
    20281 gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt
    20341 gagtgagcaa gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat
    20401 atctgttact caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc
    20461 ggcttcagat ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac
    20521 taaattggaa aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt
    20581 tattttgaaa acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta
    20641 caagaaagaa ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac
    20701 tgaagaccag gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg
    20761 aatgtgatgc tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt
    20821 tcttttcacg aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt
    20881 atcgatgctt taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga
    20941 gcagaaaaat gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa
    21001 ctgatggttg aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg
    21061 tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg
    21121 agaagaatcg gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc
    21181 cagcctgaat cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag
    21241 aaaaagagtt atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa
    21301 gtgcagcaaa atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa
    21361 gaaatagctg ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa
    21421 aaacttagca attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg
    21481 aataatgaac aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc
    21541 gcccatctag atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg
    21601 ctgcaagatg caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt
    21661 cgtttagaag aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc
    21721 gaatccattg agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct
    21781 gatttttcag aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta
    21841 cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa
    21901 aaatccattc tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt
    21961 ttcttaaaag aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac
    22021 attacgacca atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa
    22081 ctgtctcgga tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt
    22141 attatttttg atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat
    22201 aaaatttctc aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt
    22261 gctgcagtcg ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa
    22321 acttctgttt cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct
    22381 ggaagtgaga ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa
    22441 aggacctgag ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta
    22501 tttttacatg acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat
    22561 tgtccaaatg acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat
    22621 tgttttcaac ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac
    22681 aagagggctt ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat
    22741 aaataatgtt aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt
    22801 aatgttcatt ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga
    22861 agttaaaatt ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt
    22921 catttgttct actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac
    22981 caatacatca cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc
    23041 tagttctttc aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt
    23101 ttggatatcc atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc
    23161 caaagtatcg cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg
    23221 ccaaatgaat tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg
    23281 gctaaagact ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt
    23341 gggcgtcaaa ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt
    23401 tttagcaaaa ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa
    23461 ttcttctgta cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat
    23521 ttcaaattga tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca
    23581 gatcgtttgg tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata
    23641 tttgatcatg attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga
    23701 atacatggaa tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa
    23761 cagttgtgta aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca
    23821 tgggcacatt cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg
    23881 gttgccaatt tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg
    23941 atttggcgat tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa
    24001 tttctatctt accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt
    24061 tccaagcata ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt
    24121 tggtttttct gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt
    24181 acctatgaaa aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat
    24241 ttttaaaaaa atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta
    24301 gaaagtgggg gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg
    24361 ttgatgggtg aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa
    24421 cttcgtgaag aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc
    24481 ggctatccga tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct
    24541 aaaaaagacg cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt
    24601 gataaacaag gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa
    24661 ggttgcgtag tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa
    24721 gatttttctg cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc
    24781 ggactataag aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca
    24841 tgttgaaaga aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta
    24901 cactcggcgg tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc
    24961 tgtatgcgtt tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt
    25021 atgtcgaaca aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct
    25081 tgagtgagca tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc
    25141 agctagatga agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca
    25201 tggatcagtc agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat
    25261 tagtaaagat tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa
    25321 tcgaacgagt acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta
    25381 aacaagtcat cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc
    25441 aagctgtgcg gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg
    25501 caatccggtt attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag
    25561 acagaattgt caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac
    25621 ttcctgtcat acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc
    25681 ttccttcaga ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg
    25741 cagaaaaaat aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat
    25801 tacgaatatg atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct
    25861 tcgaatcaag aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct
    25921 ttggaaaaaa ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca
    25981 gttatgctgc gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa
    26041 atcaatgata aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt
    26101 actgaacgga ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta
    26161 aggaaagtga agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat
    26221 ctaaatccga tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga
    26281 ttgttctttt tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt
    26341 acctcattag ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa
    26401 agaggaacga tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca
    26461 attaaagcga tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa
    26521 aagaattttg ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat
    26581 gcactgaata ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc
    26641 tatgggaaaa atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa
    26701 ggtgtcgctg gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt
    26761 tcttctcatt ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa
    26821 ctaggtttgg agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat
    26881 caaaaagata attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc
    26941 gacggacaag atatctacac gactttagac agcagactac aaagttattt agaaacactg
    27001 atggatcaag taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca
    27061 aaaaccggag aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa
    27121 ggattgactg gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca
    27181 ggatcaacca tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag
    27241 aatgaaacct tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac
    27301 tttggtgaaa aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg
    27361 atggtcatac tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga
    27421 tttggacaaa gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta
    27481 aatattgtcg accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa
    27541 atgatgaaag ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc
    27601 agcaaagtgg tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg
    27661 caaccttttt caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa
    27721 agcgaaaact acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca
    27781 aaaaacggaa cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc
    27841 tacctgtatt cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta
    27901 acaatgaaac accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg
    27961 atgaaacggg caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca
    28021 gaaaaagtat ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct
    28081 caaaaaagtg gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct
    28141 actgccaacg gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat
    28201 aagctgatgc ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc
    28261 ctcggtattg aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca
    28321 tatgaaaaaa tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag
    28381 gagagaaaag agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat
    28441 gacaattgcc gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc
    28501 gattcgtgag gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg
    28561 cttagtgttt ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca
    28621 gctaactcct actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt
    28681 tcttgatgat tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa
    28741 attgattgga cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta
    28801 tccaggtgta ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt
    28861 cgctctcttc tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg
    28921 attagttgct ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca
    28981 gcatcagtat gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt
    29041 cgtctacaac agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg
    29101 cggactttta gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg
    29161 tttgatctat gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac
    29221 aggaaaacga atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc
    29281 ggagtggaag attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt
    29341 atggatcgtt tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga
    29401 aagtgcttgt cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat
    29461 tgggcgcact tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag
    29521 agctgctttc tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg
    29581 aagaattttc attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa
    29641 aagcccaaga aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg
    29701 aatgcccaat tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag
    29761 gactactact gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt
    29821 atccagcaag tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt
    29881 ctagcttcca actcatggga atcacagatt tccgccctca tatagcggtg attaccaata
    29941 tctatgaagc acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga
    30001 accttcaaaa aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac
    30061 tacaagaatt agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt
    30121 tggaagacgg tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg
    30181 aaatatctga acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt
    30241 ctgtagcaaa attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc
    30301 atggcgtacc acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg
    30361 attcaaaagc aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta
    30421 aggtcgtgtt attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt
    30481 cattaaaagg aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg
    30541 ctggaaaaaa agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc
    30601 ctttagcatt agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca
    30661 gctgggatca atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc
    30721 gattaaaata gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga
    30781 ggaacagggg gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa
    30841 ccgaatgctg aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct
    30901 gatacaggaa tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg
    30961 cataacatca agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc
    31021 agtgattttc aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg
    31081 tacgctgcag ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt
    31141 gttaccaata aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg
    31201 gcttcctttt ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta
    31261 gcagatacta aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca
    31321 gtcttgattt ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt
    31381 ttaagtgaat atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat
    31441 gaagagatcc taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc
    31501 aaaaatatgg cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact
    31561 tcgatcgcag aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt
    31621 acgaatgatc atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg
    31681 attgcagata atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt
    31741 gatgaagaat tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct
    31801 tcaaaacgtc tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag
    31861 tgagtaagaa aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga
    31921 ctccttggca aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc
    31981 caaaaaaaga agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg
    32041 ttcaagagga acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga
    32101 tcaagcaaaa gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt
    32161 taaaaagtca acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg
    32221 gtataccgct gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg
    32281 cagtatcagg caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat
    32341 taggagaaaa tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa
    32401 aagcacagcc aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt
    32461 tggatgtaac agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag
    32521 tgattgaaaa tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta
    32581 tcttggaaga gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt
    32641 tatcatcaga attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata
    32701 aaaatctgat ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc
    32761 ttgcgaacca aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga
    32821 tcgacatgga agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat
    32881 cgggccaatc ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa
    32941 gtgaagaggc agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat
    33001 ctgattcaaa tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt
    33061 caagtttaaa tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac
    33121 cagtaattta tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa
    33181 cggacgctag ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc
    33241 aaaaacagga atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc
    33301 tgaatatatc gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat
    33361 taaccgaggc attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg
    33421 acaagcagaa gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa
    33481 catgcttgaa gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat
    33541 caccgatgaa gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga
    33601 acgtcaaatc gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa
    33661 agatcctcgt ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc
    33721 taaaacgatc atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga
    33781 aatggtgatt actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt
    33841 tggtacgatc gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa
    33901 gcaattgaag tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc
    33961 tattgtatta aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc
    34021 atacccagaa cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga
    34081 accagtgaaa gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat
    34141 tttcaacaaa gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt
    34201 cgtattgaca ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt
    34261 cggcgtaaat gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac
    34321 aaatgtaata agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa
    34381 aattgctgtt acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac
    34441 aaacacatat gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa
    34501 agagtccgga gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta
    34561 attcatcctg ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg
    34621 cagtaatcaa agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg
    34681 aagaaaacgt taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa
    34741 attcaaaagc tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg
    34801 gttctcagcc agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag
    34861 ctttagacgg tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag
    34921 gtgctgcacc aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg
    34981 tgacacgtcc attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg
    35041 ctcgtttgaa agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag
    35101 ttgtagataa aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac
    35161 aaggtgtaca aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg
    35221 ctgacgtgaa aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta
    35281 gtggagaaga tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa
    35341 catctattga tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat
    35401 tgttcgaagc acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata
    35461 tcattctagg tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg
    35521 caacaggtat cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac
    35581 aaatgcaatc acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt
    35641 cagaagaaga aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta
    35701 gagttgatga ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg
    35761 aagaaacaaa atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa
    35821 aataaaggag aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt
    35881 caggattcgt gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca
    35941 aaatctgttg aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca
    36001 gaaaaccgag tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata
    36061 aagtggcatt taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt
    36121 gattactttc atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag
    36181 aaagaattat cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt
    36241 tcaccagatg agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt
    36301 gttgggttga tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt
    36361 ggtacgctga aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt
    36421 agtgaattaa gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca
    36481 tttatccgtg taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct
    36541 tttaaacaga ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata
    36601 ccaagattat cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca
    36661 gaatcaatca actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac
    36721 ttcacgtcct gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact
    36781 acatcaatca cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca
    36841 acctaaagca aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc
    36901 tatggcgatt gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt
    36961 agaagaaaaa caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga
    37021 tggagatatc aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat
    37081 tgacagttca accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga
    37141 actacgattt atactattgc actattactt catttacttc aacgggcagc ctatttctat
    37201 agcattatct tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg
    37261 ctaggacgat tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct
    37321 ttgaggattg gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc
    37381 gtacaaggtt tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag
    37441 caacaaaatc tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac
    37501 cagcactttc gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag
    37561 gttcaaggac aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt
    37621 ttagaaacac tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa
    37681 caggcagaaa gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat
    37741 tttgaaacag cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg
    37801 aaaatccttg gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc
    37861 tcggacggtg atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca
    37921 caagtaacaa agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg
    37981 attatgccga aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac
    38041 agtgtgatct ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct
    38101 ggtaaagtaa aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta
    38161 gatattattt cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg
    38221 aaaaaagaaa agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt
    38281 tactcatggc tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg
    38341 gatataatca agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat
    38401 tgactcaaag aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt
    38461 acttcaatga attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg
    38521 ataaagtaaa aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca
    38581 aagcagatga gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag
    38641 aagaaaaagc aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag
    38701 aaactgaaga tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg
    38761 agtctcaatt agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca
    38821 gctacgtgac tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg
    38881 aaaatgatac tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg
    38941 atacacaggc tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa
    39001 aagcactttg ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat
    39061 ggtgaaagat cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg
    39121 acctcgttat tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac
    39181 cacggcatgt tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag
    39241 gagtgtatgg ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta
    39301 tgcgtggtaa tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa
    39361 tctaccaaaa aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac
    39421 ctccatatgc caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca
    39481 ttatcattcg ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata
    39541 cacatggatt gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt
    39601 ccatggcaga atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac
    39661 gagcagattt caaacgccta ggagttgcag gagattggga acatccttat gttacattag
    39721 atccttctta tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata
    39781 tttacaaagg attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag
    39841 ctgaaattga atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag
    39901 acggcaaaga tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga
    39961 cattgccagc aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag
    40021 ccgatggccg taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg
    40081 gttgggaaaa tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg
    40141 caagtcatcc attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag
    40201 acgctggtac tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg
    40261 gtagcaaata taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag
    40321 aagcacctgg atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat
    40381 taaaagaaaa aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt
    40441 ggcggacgaa aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata
    40501 aattccgtca gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta
    40561 aaacacgttt gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg
    40621 catggggagt accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag
    40681 aaacaatcga acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa
    40741 aagaagcgaa agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat
    40801 ttacaaaaga aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg
    40861 ttttacgtca gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc
    40921 aatatcgtgg ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac
    40981 catacaaatc agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta
    41041 aatctttagg aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc
    41101 ttcgcttatg ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct
    41161 taagtcaagt ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga
    41221 atacagaaga tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag
    41281 acaaatatat gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg
    41341 acaaatataa cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat
    41401 cgtcattcta cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc
    41461 aacgtcgttg catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga
    41521 ctcctatcat tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt
    41581 acgttcagtt aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg
    41641 atacctggac tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa
    41701 gaaacagcaa attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac
    41761 aagttcgtga aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac
    41821 cagactactt tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg
    41881 atgatgttgc tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag
    41941 ttcgtaaaga tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa
    42001 tcgttgaaga cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa
    42061 aagggggtgt gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca
    42121 cgttcacgat accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt
    42181 tcgaaagctg ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt
    42241 tggtcccaag cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa
    42301 tgagagaagt tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct
    42361 gggctatttt caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca
    42421 aatccttgcc ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata
    42481 tagatcgtta gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt
    42541 ttgaatacat taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc
    42601 aaacgtttgc ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg
    42661 aatttgcctg cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga
    42721 tagcccttga attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt
    42781 tctaacactt cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta
    42841 cggatttctt tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt
    42901 aaaatatgat tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga
    42961 tcttctacac cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc
    43021 cattgagcct caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct
    43081 tttcctaaat aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc
    43141 tggttcaatt caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga
    43201 ttgaagccat tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa
    43261 aattgaggtg ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct
    43321 aatcggtcag acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat
    43381 tgatagtaaa aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg
    43441 attgtttctt tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg
    43501 attacagcaa aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac
    43561 aatttacgct gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca
    43621 ttagacatgt ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa
    43681 agctgacggg gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga
    43741 tgtcaacgat ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca
    43801 attctttttc atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca
    43861 atgtttgtcg gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag
    43921 gacaatgttc aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca
    43981 catgttccaa tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa
    44041 ctaaaaaaac ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc
    44101 ttgcagtcaa ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt
    44161 tagaaatcat ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat
    44221 tgttgacttt cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat
    44281 ttggtgtcat attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa
    44341 aaaaacgtta attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca
    44401 acaaaaaacg aaagatgata gagaagactc attctttcgt ttataaagga tatatatata
    44461 cattgggagg gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat
    44521 aaagagtttg ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta
    44581 aacgatattc atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg
    44641 tgtaatcaga tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac
    44701 cttgatactt tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa
    44761 aacttgagag aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat
    44821 cactccttta tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc
    44881 gaataaaagc taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa
    44941 ttatgatagg atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa
    45001 atagcttttt ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga
    45061 gaaataatta ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc
    45121 gctgaagtgg ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt
    45181 gctgtaaaaa agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca
    45241 attgatggta ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta
    45301 tacaagcgtg aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc
    45361 ggacaaaaag catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct
    45421 tttcctgatg cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca
    45481 caagatggtt atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca
    45541 acgatccatg aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc
    45601 ggagttcaaa tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag
    45661 gccattccat ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa
    45721 ataatgatca acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca
    45781 gctttaagca caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca
    45841 caggaatttg gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt
    45901 ttcaatatta ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt
    45961 caagctaagc aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg
    46021 acgccaaaag aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt
    46081 gaagcagcaa gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta
    46141 aaagacgaga taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa
    46201 gtattagatg gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg
    46261 gatttaggtg tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc
    46321 cattatttcc caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt
    46381 tatgagaatt ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca
    46441 gagatttatt ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca
    46501 caatttcatg atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat
    46561 ccaatggcag atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct
    46621 tggggattgt tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat
    46681 gatttaagaa aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga
    46741 acgaatttgt acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta
    46801 tctcaccttc tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag
    46861 tagcggtttc tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa
    46921 acgtggaacc aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta
    46981 ttcaagtagc agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt
    47041 tggtgggcgg agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct
    47101 tgattggtac accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc
    47161 aagtgaaaac aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa
    47221 tcattgatca aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga
    47281 cagcagatcg ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg
    47341 acgtgacagc agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga
    47401 tacgaaagaa agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga
    47461 ttttctttaa tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg
    47521 taccggtagg aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac
    47581 aattcaaaaa gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag
    47641 atattacagg tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt
    47701 tgcatcgttc gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac
    47761 aagataataa tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag
    47821 agattttttt atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc
    47881 ctgcagtaaa taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata
    47941 acaaagaaaa aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag
    48001 caaggcgcaa ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg
    48061 ccagaggagg ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa
    48121 ccaacaagga ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc
    48181 aagccgatat ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc
    48241 agtcgtttac tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc
    48301 tacgcaagtg acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca
    48361 gcaggcaaaa gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga
    48421 aactggtaca gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga
    48481 gatgctttct gcagatgcgc catttgtttc tttcaattgt gcggattatg atcaaaatcc
    48541 gcaactgctc tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca
    48601 agatagtccc ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca
    48661 ccgtttaccg ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg
    48721 tccgcttgga gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac
    48781 tgaatcttca gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt
    48841 gccgaatctt gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa
    48901 acaagaagcg aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt
    48961 catgctttat gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg
    49021 tgcaaaatct tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga
    49081 agaactttcc cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct
    49141 cgaccgattc atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt
    49201 tgtctggtca caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt
    49261 cacttctctt tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga
    49321 tgtggatgct tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga
    49381 tcttttgcca gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg
    49441 gttagaccgt atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac
    49501 attggatcgt gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa
    49561 aaattttaag agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga
    49621 acaagttgag attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct
    49681 aggagaaagt gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag
    49741 acagactgct tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa
    49801 agcttttaat atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata
    49861 tgtcaaaaac tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc
    49921 attaaacacc ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac
    49981 gatgacaagt accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt
    50041 agaagatatt tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa
    50101 agcggaaaag tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg
    50161 tgaaggagta gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt
    50221 agaactgatc cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt
    50281 attggaagaa tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat
    50341 cccatttttt tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat
    50401 agctgatgat gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga
    50461 tgtcttttct attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac
    50521 cgatttgtcg attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt
    50581 catgttagac ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt
    50641 ctcaaaaatt tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg
    50701 aaaatatcag gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga
    50761 aaataaaata aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc
    50821 gcactataaa aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc
    50881 atgcttcttg cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa
    50941 aaacaatcat gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc
    51001 aaaaagcagc agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg
    51061 cagataataa tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt
    51121 tcatgaaagc tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca
    51181 atatgcaaga ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa
    51241 tgaaataaat caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga
    51301 agccatcatg gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat
    51361 tgcggctgct aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga
    51421 atcacttata caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa
    51481 tcatatgcaa gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc
    51541 gtttactgat ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat
    51601 acaacttctt ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata
    51661 aggagaactc tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca
    51721 agttggatgg agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta
    51781 gttgctttgc gtgatgcttt tatcggcata ctgcccgcaa caatggcggg atcagtcgct
    51841 gtaatgatca atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac
    51901 agtttagcag cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt
    51961 tttgtctgga acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat
    52021 aatcttgccc gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc
    52081 ttgattcaag gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct
    52141 tcaaatgtcg cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca
    52201 ctgtcagttg gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt
    52261 acagttatga tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac
    52321 attacgatca aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt
    52381 ttaccagcga cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta
    52441 gctgatgggc aactgatcat tgatttggtg caaaaatata ttgcagaacc attcctagga
    52501 ttatctcaag ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc
    52561 tttggtattc atggatcaaa tgtattggca ccagtgctag agggaatctg gggacaagca
    52621 caactaatca atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc
    52681 gtgttagatg caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct
    52741 tttgcatggt ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc
    52801 tctaaacgtg cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat
    52861 atcaacgaac cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca
    52921 ttcttgattg ctccagtagt agcaacagca attggatgga cagctactta tcttggccta
    52981 gtcgctccgg tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta
    53041 gcaacaggag cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc
    53101 ttgatttggg caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa
    53161 caataagatg aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg
    53221 aggaggaact gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt
    53281 gttagaagga tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa
    53341 gctgttcttt gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact
    53401 aatcaaagca cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat
    53461 ggtttaaaaa gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg
    53521 ttctttgatc gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat
    53581 ggtcacaggt ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag
    53641 agaagaagca aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca
    53701 tcgttttttt acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc
    53761 aaatcagtaa ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat
    53821 aaccgggctc gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca
    53881 tcgacaacga agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc
    53941 tgtgaggttc attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat
    54001 atgcttttag gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat
    54061 atgatcgaag caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga
    54121 agtaagctag aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct
    54181 tttttgtagg aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga
    54241 ttgaggaaat gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc
    54301 gagaacgttt tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg
    54361 atcggctaac aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt
    54421 acctaatggc cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga
    54481 tcaatcagat catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt
    54541 atctggcgcc attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata
    54601 aaatcagtta tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa
    54661 taaagcgtat ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc
    54721 cggaaaacca acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc
    54781 aaaaaggcaa ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata
    54841 tcgtttatca gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg
    54901 atgctttttg cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg
    54961 aatattcatg cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt
    55021 ctgtaatgat cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa
    55081 attatccatt taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg
    55141 cgaaatggaa tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa
    55201 aagtagaaca aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat
    55261 tattagggta tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag
    55321 aatatgaaga aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt
    55381 tttgataaaa aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc
    55441 atttgaagga aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc
    55501 ttttgtttta gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg
    55561 atttttttta gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat
    55621 tggaaaggac ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta
    55681 gtggaaacgt tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa
    55741 aaacaaacaa gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca
    55801 atcgcctcct aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc
    55861 tgtatccttt gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt
    55921 tatcattttt atataaacta tcgaacacag tattgatttt ttctttatcc tgatcttcaa
    55981 gctctcgaac aatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaaac
    56041 gtttcgatac gctaagtata tttactcctt gataaagtaa aatagatgca tgtgtatgcc
    56101 ttagtccatg caaagtaaat tgcttttcaa tccctaattc ttttaatttt ttgcgcaaat
    56161 atttgctgac ggcgtttgat gaaactaatc cattttttaa gttgaaaaaa acaaaattat
    56221 gagggttttt gatttcaaaa ttctcgaata attctttttg attgatttta aactttttta
    56281 agagatcaat cgtatgttta tcaatggaaa ttttcctatt tgaagtttcg ttttttgttt
    56341 ttccccattc atttaattta taattccatg ttttattaat agtgatgatt tgatcttcga
    56401 aatcgatatc ttcccatgtt aatcctagaa gttcagcaaa tcgcattcca gtggcaccag
    56461 caactaacac gagcatagga gaagagtact gcgcagaaag gttttcttct acgagtttca
    56521 ttaaagattt aaattgatca taatctaaat atttatcttc ttttggcttc aatgaatctt
    56581 ttcctttgat tactgctttt ctagttggat caaacggaat taatccttct tctacggcat
    56641 ctttcaacga cgctctaata tggttattaa acttaataac ggtagatttt acatggtctt
    56701 tagcatactt atttaaaaag cgttgatatc ccactctatc taaatcagaa atcaataccg
    56761 ctggcatata tttctttatg ttcattaacg tatcttcgta ttttctataa gtgataggag
    56821 atactgttcc ttctttataa agttgcatcc aatcctcaaa gtaatctgat aataatagat
    56881 ttttgcgatc ccctttaagg cctttgttca agttatattc taattcgttg gcggcatctt
    56941 ttgcttctcc ttttgtctta aatcctgatt ttcttatctt cgtgtatttt ccgtcatctt
    57001 ttttgtatga aatttcgtat tgccaactat ttccacgttt gactaatctt gccataattg
    57061 aatcagctct ctttctctga tacaatagga actataaaga agcccattgt ttgggtttta
    57121 ttattttttg cacatccttt ttgcttgccg gcgcaaggat gtgctatttg tcgttttaat
    57181 ggtataattg gatgtgtatt agatcatcca atcagaggtg tttattatga ttaaagtgat
    57241 ttttaaaaga ataactgaac ttccagagtt tagattattt gttgattatt ggtatgtctg
    57301 gttttttatt gttgcattgc tgataggtct agatgtcata ttagaaagaa aacttaagta
    57361 agagttgagt taataaattg aattcctaaa tgagatattt tgtagcccat attttgtttg
    57421 ccgacatcac ctaatactat ggagctaatc gttttttcta acagaccaaa atcagataat
    57481 ctatttaagc taccatctat tagctcttgt gaatagccag acatgctgat atttccagtg
    57541 aaattttcgt ttccacgact cattaagcat gctaacaatt gaatctcaat cgatgttaat
    57601 tgattcagta caagaagata atactcctcg ttatcccatg aagcgttgtt aattcgaagt
    57661 aaacaattct tataaagatt tttatacata tctattttat tttgtgcctt tgatttttct
    57721 acctcgtcat ttatcccttc gaatattcca atgagttggt ccttgttctc taagttgaaa
    57781 acatcttctg gaatttgatc gcgaatgttt tctaatgatt cgtttaaatt tttgtagaat
    57841 ttctcgatgc gtttgaaacg cttctcgttt ttagacccaa aataaagtga ctgcacaacg
    57901 ccaccaatat aaggaattgc tccaataccg ccttcaacta ttaattcaag atattctttt
    57961 ttggttagtt tttcattgct attatccata gttacctcct attttgtcat ttcctttaca
    58021 agcattgtga ctcctgatgg cactcttttg tataggtttt attattgtgc acaatctaac
    58081 tcttagtggg gcgtggttca tttatttttt acttacttta ctattaatca tatcccattg
    58141 agcataggat ttttctatgt tcgatcctct tgaactaatt tctcctgtaa aagttacttt
    58201 atcgcccttt gtaaaggtat cagcattgtt taagtccttc gcaaaaatta catatgaaac
    58261 tttaggtgtt gcagaaatat cattccaact tttatcatcg tactctttgt ctgctataat
    58321 ggctattcta gtagtcattg attcgataac agttccttca aaagtatatg ttttaccact
    58381 aatatattta gtaaaagctg tattttgctc agtagctgga gtaatagaat aataactatc
    58441 taagaaagca ccccagtcat gattatttgt ctctaaaaat tcttcaaatt gagttaggct
    58501 tttattgctg ctgttagatg tagtagtttg agtatcagat gatgagcttt cctgtttgtt
    58561 attgtcattt gcctcgtttg atgaggaaag cgagaaacct acggtagtaa aaataatccc
    58621 gataataagg agaatcatag gtgttttctt atttgttttt ttgataaatc ttacaattaa
    58681 catgattaaa cctataataa ataaaacaat accagtaaaa ccaaatatac cgtccattaa
    58741 tagatcctct ttctcttttg atattgaata ttataaacca agcaattctt ttttcttaaa
    58801 atcgaattct tcttgattaa taatcccttc atctaacaac tctttatact ttttaatttc
    58861 atctgcactt gaaatttgtg t
//
LOCUS       DAZKMB010000006        51395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000006 DAZKMB010000000
VERSION     DAZKMB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.006"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..1043
                     /locus_tag="ACG8V3_000313"
     CDS             165..1043
                     /locus_tag="ACG8V3_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0298885.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            1519..1635
                     /locus_tag="ACG8V3_000314"
     CDS             1519..1635
                     /locus_tag="ACG8V3_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0298886.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            2563..2901
                     /locus_tag="ACG8V3_000315"
     CDS             2563..2901
                     /locus_tag="ACG8V3_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0298887.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            2995..3225
                     /locus_tag="ACG8V3_000316"
     CDS             2995..3225
                     /locus_tag="ACG8V3_000316"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0298888.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            3625..3996
                     /locus_tag="ACG8V3_000317"
     CDS             3625..3996
                     /locus_tag="ACG8V3_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0298889.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(4270..4962)
                     /locus_tag="ACG8V3_000318"
     CDS             complement(4270..4962)
                     /locus_tag="ACG8V3_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0298890.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(4965..5750)
                     /locus_tag="ACG8V3_000319"
     CDS             complement(4965..5750)
                     /locus_tag="ACG8V3_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0298891.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            5987..7387
                     /gene="lacG"
                     /locus_tag="ACG8V3_000320"
     CDS             5987..7387
                     /gene="lacG"
                     /locus_tag="ACG8V3_000320"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0298892.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7412..7729
                     /locus_tag="ACG8V3_000321"
     CDS             7412..7729
                     /locus_tag="ACG8V3_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0298893.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            7742..9415
                     /locus_tag="ACG8V3_000322"
     CDS             7742..9415
                     /locus_tag="ACG8V3_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0298894.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            9429..11060
                     /locus_tag="ACG8V3_000323"
     CDS             9429..11060
                     /locus_tag="ACG8V3_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0298895.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(11551..13116)
                     /gene="guaA"
                     /locus_tag="ACG8V3_000324"
     CDS             complement(11551..13116)
                     /gene="guaA"
                     /locus_tag="ACG8V3_000324"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0298896.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(13175..14098)
                     /gene="coaA"
                     /locus_tag="ACG8V3_000325"
     CDS             complement(13175..14098)
                     /gene="coaA"
                     /locus_tag="ACG8V3_000325"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0298897.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            14294..14899
                     /locus_tag="ACG8V3_000326"
     CDS             14294..14899
                     /locus_tag="ACG8V3_000326"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0298898.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            15207..16223
                     /locus_tag="ACG8V3_000327"
     CDS             15207..16223
                     /locus_tag="ACG8V3_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0298899.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            16364..17665
                     /locus_tag="ACG8V3_000328"
     CDS             16364..17665
                     /locus_tag="ACG8V3_000328"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0298900.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            17711..18373
                     /gene="deoC"
                     /locus_tag="ACG8V3_000329"
     CDS             17711..18373
                     /gene="deoC"
                     /locus_tag="ACG8V3_000329"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0298901.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            18394..18792
                     /locus_tag="ACG8V3_000330"
     CDS             18394..18792
                     /locus_tag="ACG8V3_000330"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0298902.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            18858..19940
                     /locus_tag="ACG8V3_000331"
     CDS             18858..19940
                     /locus_tag="ACG8V3_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0298903.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            20152..21714
                     /locus_tag="ACG8V3_000332"
     CDS             20152..21714
                     /locus_tag="ACG8V3_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0298904.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            21707..22840
                     /locus_tag="ACG8V3_000333"
     CDS             21707..22840
                     /locus_tag="ACG8V3_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0298905.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            22840..23796
                     /locus_tag="ACG8V3_000334"
     CDS             22840..23796
                     /locus_tag="ACG8V3_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0298906.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            23895..25061
                     /gene="deoB"
                     /locus_tag="ACG8V3_000335"
     CDS             23895..25061
                     /gene="deoB"
                     /locus_tag="ACG8V3_000335"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0298907.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            25085..25903
                     /locus_tag="ACG8V3_000336"
     CDS             25085..25903
                     /locus_tag="ACG8V3_000336"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0298908.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            25920..26627
                     /gene="deoD"
                     /locus_tag="ACG8V3_000337"
     CDS             25920..26627
                     /gene="deoD"
                     /locus_tag="ACG8V3_000337"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0298909.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(26925..27611)
                     /gene="gpmA"
                     /locus_tag="ACG8V3_000338"
     CDS             complement(26925..27611)
                     /gene="gpmA"
                     /locus_tag="ACG8V3_000338"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0298910.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(27783..28364)
                     /locus_tag="ACG8V3_000339"
     CDS             complement(27783..28364)
                     /locus_tag="ACG8V3_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0298911.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(28497..29180)
                     /gene="rpiA"
                     /locus_tag="ACG8V3_000340"
     CDS             complement(28497..29180)
                     /gene="rpiA"
                     /locus_tag="ACG8V3_000340"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0298912.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            29499..29912
                     /gene="rpsL"
                     /locus_tag="ACG8V3_000341"
     CDS             29499..29912
                     /gene="rpsL"
                     /locus_tag="ACG8V3_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0298913.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            29996..30466
                     /gene="rpsG"
                     /locus_tag="ACG8V3_000342"
     CDS             29996..30466
                     /gene="rpsG"
                     /locus_tag="ACG8V3_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0298914.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            30560..32647
                     /gene="fusA"
                     /locus_tag="ACG8V3_000343"
     CDS             30560..32647
                     /gene="fusA"
                     /locus_tag="ACG8V3_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0298915.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            32819..34006
                     /gene="tuf"
                     /locus_tag="ACG8V3_000344"
     CDS             32819..34006
                     /gene="tuf"
                     /locus_tag="ACG8V3_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0298916.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            34413..34721
                     /gene="rpsJ"
                     /locus_tag="ACG8V3_000345"
     CDS             34413..34721
                     /gene="rpsJ"
                     /locus_tag="ACG8V3_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0298917.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            34752..35381
                     /gene="rplC"
                     /locus_tag="ACG8V3_000346"
     CDS             34752..35381
                     /gene="rplC"
                     /locus_tag="ACG8V3_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0298918.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            35409..36032
                     /gene="rplD"
                     /locus_tag="ACG8V3_000347"
     CDS             35409..36032
                     /gene="rplD"
                     /locus_tag="ACG8V3_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0298919.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            36032..36322
                     /gene="rplW"
                     /locus_tag="ACG8V3_000348"
     CDS             36032..36322
                     /gene="rplW"
                     /locus_tag="ACG8V3_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0298920.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            36361..37194
                     /gene="rplB"
                     /locus_tag="ACG8V3_000349"
     CDS             36361..37194
                     /gene="rplB"
                     /locus_tag="ACG8V3_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0298921.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            37237..37515
                     /gene="rpsS"
                     /locus_tag="ACG8V3_000350"
     CDS             37237..37515
                     /gene="rpsS"
                     /locus_tag="ACG8V3_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0298922.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            37538..37885
                     /gene="rplV"
                     /locus_tag="ACG8V3_000351"
     CDS             37538..37885
                     /gene="rplV"
                     /locus_tag="ACG8V3_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0298923.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            37899..38555
                     /gene="rpsC"
                     /locus_tag="ACG8V3_000352"
     CDS             37899..38555
                     /gene="rpsC"
                     /locus_tag="ACG8V3_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0298924.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            38558..38992
                     /gene="rplP"
                     /locus_tag="ACG8V3_000353"
     CDS             38558..38992
                     /gene="rplP"
                     /locus_tag="ACG8V3_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0298925.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            38982..39170
                     /gene="rpmC"
                     /locus_tag="ACG8V3_000354"
     CDS             38982..39170
                     /gene="rpmC"
                     /locus_tag="ACG8V3_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0298926.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            39195..39461
                     /gene="rpsQ"
                     /locus_tag="ACG8V3_000355"
     CDS             39195..39461
                     /gene="rpsQ"
                     /locus_tag="ACG8V3_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0298927.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            39519..39887
                     /gene="rplN"
                     /locus_tag="ACG8V3_000356"
     CDS             39519..39887
                     /gene="rplN"
                     /locus_tag="ACG8V3_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0298928.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            39924..40232
                     /gene="rplX"
                     /locus_tag="ACG8V3_000357"
     CDS             39924..40232
                     /gene="rplX"
                     /locus_tag="ACG8V3_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0298929.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            40260..40799
                     /gene="rplE"
                     /locus_tag="ACG8V3_000358"
     CDS             40260..40799
                     /gene="rplE"
                     /locus_tag="ACG8V3_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0298930.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            40818..41003
                     /locus_tag="ACG8V3_000359"
     CDS             40818..41003
                     /locus_tag="ACG8V3_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0298931.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            41039..41437
                     /gene="rpsH"
                     /locus_tag="ACG8V3_000360"
     CDS             41039..41437
                     /gene="rpsH"
                     /locus_tag="ACG8V3_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0298932.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            41469..42005
                     /gene="rplF"
                     /locus_tag="ACG8V3_000361"
     CDS             41469..42005
                     /gene="rplF"
                     /locus_tag="ACG8V3_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0298933.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            42166..42522
                     /gene="rplR"
                     /locus_tag="ACG8V3_000362"
     CDS             42166..42522
                     /gene="rplR"
                     /locus_tag="ACG8V3_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0298934.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            42543..43043
                     /gene="rpsE"
                     /locus_tag="ACG8V3_000363"
     CDS             42543..43043
                     /gene="rpsE"
                     /locus_tag="ACG8V3_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0298935.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            43058..43237
                     /gene="rpmD"
                     /locus_tag="ACG8V3_000364"
     CDS             43058..43237
                     /gene="rpmD"
                     /locus_tag="ACG8V3_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0298936.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            43281..43721
                     /gene="rplO"
                     /locus_tag="ACG8V3_000365"
     CDS             43281..43721
                     /gene="rplO"
                     /locus_tag="ACG8V3_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0298937.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            43721..45016
                     /gene="secY"
                     /locus_tag="ACG8V3_000366"
     CDS             43721..45016
                     /gene="secY"
                     /locus_tag="ACG8V3_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0298938.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            45078..45725
                     /locus_tag="ACG8V3_000367"
     CDS             45078..45725
                     /locus_tag="ACG8V3_000367"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0298939.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            45916..46134
                     /gene="infA"
                     /locus_tag="ACG8V3_000368"
     CDS             45916..46134
                     /gene="infA"
                     /locus_tag="ACG8V3_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0298940.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            46167..46283
                     /gene="rpmJ"
                     /locus_tag="ACG8V3_000369"
     CDS             46167..46283
                     /gene="rpmJ"
                     /locus_tag="ACG8V3_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0298941.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            46301..46666
                     /gene="rpsM"
                     /locus_tag="ACG8V3_000370"
     CDS             46301..46666
                     /gene="rpsM"
                     /locus_tag="ACG8V3_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0298942.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            46694..47083
                     /gene="rpsK"
                     /locus_tag="ACG8V3_000371"
     CDS             46694..47083
                     /gene="rpsK"
                     /locus_tag="ACG8V3_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0298943.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            47163..48101
                     /locus_tag="ACG8V3_000372"
     CDS             47163..48101
                     /locus_tag="ACG8V3_000372"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0298944.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            48129..48509
                     /gene="rplQ"
                     /locus_tag="ACG8V3_000373"
     CDS             48129..48509
                     /gene="rplQ"
                     /locus_tag="ACG8V3_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0298945.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            48781..49620
                     /locus_tag="ACG8V3_000374"
     CDS             48781..49620
                     /locus_tag="ACG8V3_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0298946.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            49596..50465
                     /locus_tag="ACG8V3_000375"
     CDS             49596..50465
                     /locus_tag="ACG8V3_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0298947.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            50455..51252
                     /locus_tag="ACG8V3_000376"
     CDS             50455..51252
                     /locus_tag="ACG8V3_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0298948.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        16371 a         9266 c        11135 g        14623 t
ORIGIN      
        1 ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag
       61 tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag
      121 tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata
      181 tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt
      241 taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg
      301 aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa
      361 agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg
      421 cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt
      481 tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct
      541 atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg
      601 gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag
      661 gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa
      721 gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc
      781 aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag
      841 agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg
      901 atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa
      961 gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg
     1021 gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac
     1081 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
     1141 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
     1201 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac
     1261 acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag
     1321 agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc
     1381 aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca
     1441 tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga
     1501 agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa
     1561 agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat
     1621 agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg
     1681 gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca
     1741 tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt
     1801 ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt
     1861 agttattttt caattccctt tcaacctctt attttgacca gtagtattga tcactttacc
     1921 ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat
     1981 aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt
     2041 gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca
     2101 gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga
     2161 aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac
     2221 tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt
     2281 tgtgtattgg aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca
     2341 ctattacaag tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa
     2401 tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc
     2461 aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt
     2521 gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca
     2581 agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat
     2641 attgttccgg agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata
     2701 ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca
     2761 gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact
     2821 gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc tactgataaa
     2881 aagtatatat tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa
     2941 atttactaga tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg
     3001 gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa
     3061 aatgagtgga aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt
     3121 tacagagaag gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta
     3181 gctgaagtag aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat
     3241 gaaaaatttt gacacatatt atgttattgt tttttcgata tctttgctgt taatattact
     3301 aactttaatg cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt
     3361 aagtagctac acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt
     3421 cttaattagc ttattgattc tataatattt ttgatttata taggaaatta aaaatattat
     3481 agaatcaaca ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa
     3541 atttctcctg gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac
     3601 tttgcggagt atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc
     3661 atcaatcaat cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc
     3721 attatttctt cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat
     3781 aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat
     3841 cttaagttta atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa
     3901 gatacggaaa cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact
     3961 cagcttggtg gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa
     4021 gtatatcaaa aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac
     4081 actattttta atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa
     4141 aagtcaaaga tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt
     4201 tcactggcag ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag
     4261 ttgaatcgtt taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa
     4321 atctttgatg gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc
     4381 gaatggatca attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga
     4441 gtaagcatct tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt
     4501 aagaaaaata tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc
     4561 ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac
     4621 agtagccgtg gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc
     4681 aagccctttt gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct
     4741 ttctttttct tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg
     4801 tttcaaaatc tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa
     4861 accatctttt gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc
     4921 caaaaacatt gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg
     4981 gtattttcgg ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc
     5041 tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca
     5101 taaaaatcat ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta
     5161 tttagagcga tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg
     5221 tgaacgccat ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg
     5281 gtgatcggtc cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt
     5341 tctggtccat ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga
     5401 attttcttgt tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa
     5461 atcgtttctc cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga
     5521 atctgcagag tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt
     5581 tgagcccctc catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg
     5641 cgaacggtca tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct
     5701 tttttattga ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc
     5761 tctaatttaa ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag
     5821 taaacattta gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt
     5881 ttatgattat ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc
     5941 agttagaaag agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc
     6001 agaagatttt atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga
     6061 aggtggcaaa ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc
     6121 cgatccggca agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt
     6181 tggtgtaaat ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg
     6241 gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag
     6301 aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca
     6361 tggagatttt ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt
     6421 tcatgaattc aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac
     6481 caatcaatat ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt
     6541 cgcctgtctt cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa
     6601 cgaacttccg ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga
     6661 cgcgccggaa gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt
     6721 agatgctacc tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg
     6781 tgaagcaaat caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc
     6841 atccacgcgt aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta
     6901 cgatggagaa aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa
     6961 agtgaaggga atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg
     7021 gctgatttat ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca
     7081 ttacaacaaa atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg
     7141 cattattatg gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa
     7201 ggcgataaca gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt
     7261 ctcatggaca aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca
     7321 gaagcgttac ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat
     7381 tatttaataa gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg
     7441 ttttgagatc gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga
     7501 agtaagacaa ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt
     7561 aacccttgca cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat
     7621 ggagatggga ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag
     7681 agatctgatt caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc
     7741 tatggatgga atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg
     7801 taatagttat ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt
     7861 ttcaagcatt ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga
     7921 atctgtctcc acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact
     7981 cgtttcagca accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa
     8041 caaagtaatc aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag
     8101 cgtctctccg attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc
     8161 ttcctttgtc gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat
     8221 taccattaaa atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt
     8281 tcctttctct ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt
     8341 ccaaatctca tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga
     8401 cgggtactta ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt
     8461 tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac
     8521 caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg
     8581 gaattttgta ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct
     8641 actctttgct aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag
     8701 tttcgctgtc aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt
     8761 tattccattt ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt
     8821 attgggcatg aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt
     8881 ggttctagga acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt
     8941 tgacttcctg atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga
     9001 agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga
     9061 agtacctgtg gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt
     9121 actttgtgct ggcgcaggga caagtgggat gttagctaat gctttaaaaa aaggtgcatc
     9181 tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct
     9241 gcaggatttt gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa
     9301 agaagacacc gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg
     9361 attaacgaga gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag
     9421 gagccataat gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag
     9481 gggctttggg ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag
     9541 cagatatttc gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata
     9601 tttcaaaaga tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt
     9661 tgtggcagga tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg
     9721 aaacgacgat tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt
     9781 ttcactacag tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag
     9841 gtctgacctt tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg
     9901 aaatgaagca gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg
     9961 gaatgctctg gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg
    10021 ctgctttttt gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag
    10081 taatgctcca cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa
    10141 tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg
    10201 gttcaatgaa tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag
    10261 tgatcgtagt tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg
    10321 cttttggtga agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag
    10381 agtatttgct ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct
    10441 tcttctattg ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta
    10501 tgaattatct cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg
    10561 attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa
    10621 atctagttaa aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc
    10681 aaacctggtt tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt
    10741 ttggggacta caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa
    10801 atgttagtgg cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag
    10861 agtttgatac taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg
    10921 ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg
    10981 tttgtgaaat tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg
    11041 tttcattggt gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca
    11101 gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa
    11161 ccagaaacga tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac
    11221 tttgtattat gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa
    11281 agttgtgcag ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa
    11341 tatgatttga gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag
    11401 aacttctttt tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg
    11461 gaaccatctt atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata
    11521 gggattcccc ctccatatta tgttggctct ttattcccac tccactgttg ctggtggttt
    11581 agaagtgatg tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga
    11641 gattttttgc aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga
    11701 agttacagca cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac
    11761 agagcggatc cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc
    11821 tgcgatttct tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt
    11881 gatttcgcca agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat
    11941 tgaatcaggc atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt
    12001 taatggttcg atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga
    12061 tttgatcgtc tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg
    12121 tgctaggaaa gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata
    12181 tacgaattca ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt
    12241 gtctaagaag cgatctcttg cattgacttt gatgatgttc aagccgaatt ttccgcctag
    12301 actttccatt acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga
    12361 tgtcaattgg tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc
    12421 acctgaaaga ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg
    12481 catatcgata aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa
    12541 gtggcgcagc aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc
    12601 ataaaaatta cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac
    12661 tgtttcaaag ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg
    12721 ttgttttggt gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt
    12781 cccatattcg cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg
    12841 cataccatag caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc
    12901 aaatgcattc tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt
    12961 catcgctttt acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag
    13021 atcacgaata cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat
    13081 cttttcaacg ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa
    13141 ttttatctca aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat
    13201 cgatgatatg attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga
    13261 tatattcctg taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg
    13321 cttcttcacg atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg
    13381 tatccagtaa cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat
    13441 cgacatagac agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt
    13501 gaagcgtatt gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt
    13561 ctggaatgat atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag
    13621 attttacacg gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt
    13681 tacggtccat gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg
    13741 tgatcagctg aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg
    13801 ctgttgtact ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt
    13861 ctacgtattt atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat
    13921 aaagataaat cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc
    13981 ggtcattcaa acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac
    14041 gataaaattc tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat
    14101 tagcctcctg cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca
    14161 ccacttaaga gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc
    14221 ttattcttga cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag
    14281 aaggatggaa gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt
    14341 tgaacaatgg gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt
    14401 attttcacgt gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga
    14461 agagttacct gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt
    14521 agctttggca tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt
    14581 aagtctagcg caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc
    14641 gaatatctat gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat
    14701 ccgtgctgga aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa
    14761 gggcggtaca ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa
    14821 aatggaagaa acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat
    14881 caaaagtgtg aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg
    14941 cgtgcatgta cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa
    15001 ctattttttc ttcaaaaaga aatagtagga acagagtcgt tgttttttac gataaaataa
    15061 ataagataca gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca
    15121 aaaaacatac tttccttttt tctctattct tttcttgggg gttttggtac actgataggg
    15181 aatttagata gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg
    15241 caggcgtatc agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg
    15301 ataagacaag aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt
    15361 tcgcttcaaa tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca
    15421 ctgatttctt cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct
    15481 atgtgatcat gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag
    15541 agctttccca ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga
    15601 aatatgcatt agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat
    15661 tgaatcgtcg ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg
    15721 tttcgctgtt tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat
    15781 actatcaatt agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc
    15841 cttttaatgg gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat
    15901 caaaaaaatt actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg
    15961 cgatcggatg ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag
    16021 tcataggatt cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc
    16081 agcagccaag ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat
    16141 ttcctcaaag aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg
    16201 taaaagtatt gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg
    16261 ctagttagtg ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta
    16321 aaaataatgt ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct
    16381 gattgaaaaa aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac
    16441 gaactatacg aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt
    16501 ttatcaagac atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg
    16561 tgatgtcatc gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg
    16621 cgttggtgac acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt
    16681 ggctaaaatg tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc
    16741 aattcccgga tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag
    16801 taaagtagcg gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc
    16861 cttgcgtgac gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag
    16921 taaaaaaatc gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc
    16981 tttcatgaag aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa
    17041 actggctaac cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc
    17101 tatcggaaat agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga
    17161 agatctagta gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc
    17221 taagacaacc gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt
    17281 agcaaaattc aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc
    17341 agaacgaatt ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt
    17401 gtcaaaaatc gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa
    17461 aaccaaagaa gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga
    17521 cactgtgaca aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc
    17581 tgtcattgaa ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt
    17641 gattcatgac atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag
    17701 gggaaaagaa atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc
    17761 taaagaagct gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg
    17821 catcaatcca gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt
    17881 atgtacagta atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac
    17941 aacagatgcc atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt
    18001 gaaatcaaag gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa
    18061 agatcgtgcg cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat
    18121 ccgagcttgc gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt
    18181 ctcaacaggc ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga
    18241 aatgggtgtc aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga
    18301 agctggtgct acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg
    18361 tgaaggctat taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc
    18421 aacacagcta ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc
    18481 gcttgtctcg taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca
    18541 tttggattaa cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa
    18601 aaaacattta cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt
    18661 gcatgtcgac aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga
    18721 gacaatggtg tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa
    18781 aaagatctat aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt
    18841 taggaggctt tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag
    18901 cagcagggtt atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca
    18961 aaagctcatc agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc
    19021 gttcattcaa ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt
    19081 cacgaggaaa tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa
    19141 atattgacca agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa
    19201 aaccagcgat ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg
    19261 atgtcatcga tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt
    19321 atcttgcagg tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg
    19381 gtgtaaaagg tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag
    19441 gtgcgaaaga attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg
    19501 ctccagataa aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct
    19561 tccatgcttc aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat
    19621 caggcgacaa aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat
    19681 acacattaaa cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa
    19741 cagtagtaga agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca
    19801 ctgtttatgg attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag
    19861 cgaaaaaagc agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag
    19921 aaactcctga agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg
    19981 ctatggtttg attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga
    20041 taagggaaca actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga
    20101 atcttagtca atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa
    20161 gaaaatattg tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat
    20221 gacaacatca atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt
    20281 gctggcaaat ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa
    20341 attttgatgc gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg
    20401 attgggatgg tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc
    20461 gtgttgggaa gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag
    20521 atccaaaaag tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat
    20581 atttcagttg gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat
    20641 gtactgattt ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc
    20701 gtgatcatga aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta
    20761 gatgaaatca aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt
    20821 acagtcaacg ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg
    20881 gtttcattca aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa
    20941 aatctagtgg tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt
    21001 cgtgctggcg aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc
    21061 caagctttga ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac
    21121 atcactaata aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac
    21181 cgtcataaat acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc
    21241 tatcatcaaa aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat
    21301 gccagagaat tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa
    21361 gctttatcag gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct
    21421 gatctgttga ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt
    21481 cataaacgtc tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa
    21541 ttagaagaaa ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc
    21601 ggtatcgttg atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat
    21661 accttagaag aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag
    21721 cgaaaaattc cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg
    21781 cgcgatattg atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga
    21841 tgcagccctt ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat
    21901 ttttacagca ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc
    21961 tggacaagca ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct
    22021 gccaaaagtt gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc
    22081 agcggctgtt ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat
    22141 catgatgaac tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga
    22201 aagctatcgc tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt
    22261 accatggtta acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat
    22321 catcgcgctt attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg
    22381 ttctgttgga ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat
    22441 cgttttgtcg atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt
    22501 aggaacgtat caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat
    22561 ggctgtttcc ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt
    22621 cagtattttg aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat
    22681 cgtcaatgta tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt
    22741 cgtcatggct aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc
    22801 gaaaccaacg aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt
    22861 gccttgatcc ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc
    22921 ggaacttttt ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg
    22981 ggtgctttta gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca
    23041 ccatggatcg ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca
    23101 gtagcaacag tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg
    23161 gcacctgctt taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac
    23221 atcacgcaga atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga
    23281 ccaattttct ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta
    23341 gcttgggtga tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat
    23401 cctcaagctg ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg
    23461 atttctggct tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac
    23521 ttttcagctg gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt
    23581 aagtggaatc ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg
    23641 agtatcattg gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt
    23701 gctccatatt tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca
    23761 aaagcaaacg ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg
    23821 gttacgggaa gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga
    23881 aagaaggaat aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg
    23941 gagaagcacc agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg
    24001 cgaaagaagc aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc
    24061 ctcttgaagg tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag
    24121 tttctgtagg gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa
    24181 ctccatttcg tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat
    24241 tttctggccg taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt
    24301 acggtgaaca ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc
    24361 ttcagatcgc ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat
    24421 tcgttcgtga tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt
    24481 acgtgggtga accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc
    24541 cattcggaca cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg
    24601 gaaagatcaa cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca
    24661 atatggatgg cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct
    24721 ttaccaattt agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg
    24781 cgcatgcgat tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag
    24841 acgacttgct aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc
    24901 atacgcgaga atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat
    24961 tgcctcaagg atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt
    25021 cgactgaaaa tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa
    25081 gcaaatgacc aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg
    25141 agtaaaagaa attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga
    25201 aatcgaagat gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt
    25261 agttgggcat gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat
    25321 gcaagggaga ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg
    25381 tgtgatggcc ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa
    25441 cgaatcattt gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga
    25501 aaatcctttg atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca
    25561 agcttatacg cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga
    25621 tcttaaagaa ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat
    25681 ccgaatgtct cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat
    25741 cgtagcagct cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc
    25801 aggtatgcaa gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca
    25861 atcttttaaa gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa
    25921 tgagtgtgca tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag
    25981 atcctttgcg agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc
    26041 aggtacgcgg tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag
    26101 gaacaggaat gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg
    26161 gtgtaaagaa attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc
    26221 gtgatttagt actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc
    26281 aagcctttca ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag
    26341 ctaaagaaaa gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca
    26401 aagatgattt gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg
    26461 aagcagcggc tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga
    26521 ccgttagcga ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt
    26581 tcaatgaaat gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa
    26641 aagctgccct gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga
    26701 cttggctgga cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg
    26761 aatctgccgt ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg
    26821 atgaatattg tgacttgtga ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag
    26881 ggactgtgac aaatcgtgtc acagtccctt ttttatagtg ttttttatag gtagtatttt
    26941 ttagcaactg ttaagtcatc atttaattca taaacaagtg gttgacctgt tggaatttca
    27001 agatccatga tgtcttcgtc agagatacct tcgatatgtt tagctagggc gcgtaatgag
    27061 ttaccatgtg cagctactaa aacagttttg ttgtctagta atgctggagc aatttcatct
    27121 tgccagaatg gcaaagcgcg ttctaatgtc actttcaagt tttctccacc aggaatatcg
    27181 cgtttgtcta acattgcgta acgacggtca tttgctgcag aaccttcgtc agtttcttcc
    27241 attaatggag gaagagtatc gtaagaacga cgccagatgt gcacttgttc gtcgccgtat
    27301 ttttcagctg tttctttttt gttcaaacct tgtaatttac catagtgacg ttcgtttaag
    27361 cgccatgatt tgatttgtgg tacccaaagt tgatctgaat attctaagat caagttacat
    27421 gttttgattg cacgagttaa aacagaagtg taagcaacat caaattcgat tccagcttct
    27481 ttgattttgc gtccgccttc ttttgcttct tcgattcctt ctggtgctag atttacatca
    27541 gcccaaccag taaattggtt caatgcattc cattcactaa gaccgtgacg agaaaaaact
    27601 aattttggca tgtttagctc tccttttaag ataggtttat atttctgaaa ttttcagttc
    27661 tgttttattg tacaattttt tggaagagat ttccatcgaa aaattagata agattctatg
    27721 agaaaacaag tagagaatat aaaaaaacga acagtgccca cgcaactgtt cgtttttagg
    27781 tattagatgc ataattgctc gatttctttt tcaggtaaat tgatatattt ataacatgtt
    27841 ccaactgaaa caccgcattt tgaagaaata aattggatcg tttctttttt ttcataatag
    27901 agacgtcgga tttttttgat cgtttttgca tcgatttttg gtcttccgcc gatttttcct
    27961 tttttacgag cttcatctaa tccaactaat gtacgttctt tgatcaatgc gcactccatg
    28021 tttgctaggt tttccattaa ttggaaatag atttttccca tttcgcgcgt atcgattcct
    28081 tcatcaagac taatcaagtg cagcccgcga tctttgaata attgcgtgag ttctgttaat
    28141 tgccgcgtag atttgcctaa tcgatgaagt tggcaaatga ctagtgcatc gccttgttgc
    28201 atgtcagcga tcactgactc caaatgaaca gcagtagaat cattttgttc cacttcatat
    28261 gtctcatgga aaatttcttg acaaccaaag tcagataatt gatcaacctg agttgttaaa
    28321 tcaacgtcat ttatggttgt gcgtgcataa ccaataattt tcatggtgat tcctttctag
    28381 cagtgatttt ctaactggat ataacagtaa aaatgtagca taaagtacgt caaaaagata
    28441 gttttttcgc aaattttccg ctgtcttttt ttattggatg atcaagcagt ttttctttat
    28501 ttacgacgga ttgcgtcaag aatctcaacg cctttttctg agccgacaac tactttagaa
    28561 accatgttta ggaaaagccc atgttctact accccaacaa gttcatctaa ataggtagct
    28621 aagctttctg gctgttcgat gacttctaga tgcagatcaa tgatgtaatg accaccatct
    28681 gtggttaaga cttctccgtt ttcgtctgta cgcaagacgg gttggaatct tttttcttca
    28741 aaaatatgaa gtagctgttg ggagccataa gggatcactt caactgggag tgggaacttc
    28801 ccaagtttat cgactaattt agaagaatca acgatccaaa tcgtttcttt tgagtacgta
    28861 gcgactattt tctcgaaaag caaagcagcg ccgccgcctt tgatcccttg aaaatcagca
    28921 ctgatttcat cagcaccgtc tattgtcaaa tcgactacgg ggacttcatc aatactcttt
    28981 aacggtattc ctaatttttc cgcttgttct tttgtcgcat ttgaagtagt aactcctgtg
    29041 atacgtaaac cttcttcacg catacggcga ccaatctctt ctaccatata ataggcggtt
    29101 gaaccggtcc cgagacctac gatcatcccg tcctttacga atttcgcggc ttcaatgcca
    29161 gctaattgtt ttaaattcat ctttgcgtcc accttttttt agtttgagca tcgtgctttc
    29221 ctcactattt tagcgtgatt tacggtaaaa agataccatt cttttttatt ccaataaagc
    29281 tttttcaaaa aaaattgcat ctcttgttga catgtttttt cattcgtgat attctaacaa
    29341 aggtgcttta tctacaggtt tctgtgaaga aacgtgctga ctgtttatca aagcatattt
    29401 gataaggatg cagacattgc atttattttt tatcacaaaa aaagaaccac ctggatgtgt
    29461 ggaactagac gcaaatcgtg ggaaggagga aacaaggaat gcctacaatt aaccaattag
    29521 tacgtaaacc tcgtaaatca aaggtggaaa aatctaattc acctgctttg aacaaaggat
    29581 ataacagctt taagaaaact caaactaacg taaactcacc tcaaaaacgt ggggtagcaa
    29641 ctcgtgtggg tacaatgaca cctaaaaaac cgaactcagc tttacgtaaa tatgcacgtg
    29701 ttcgtttgtc aaacttaatt gaagttactg cttatatccc agggattggt cataacttac
    29761 aagaacacag tgttgtatta ttacgcggtg gtcgtgtaaa agacttacca ggggtacgtt
    29821 accatatcgt tcgcggtgct ttagatactg ctggtgttaa cgaccgtaaa caaagccgtt
    29881 ctaaatacgg aactaaaaaa cctaaagctt aattttaatt attaaacaaa tgaaacttgc
    29941 ttctactata tagaagtaat tactatgaac aatatttcgg aaggaggagt tacggatgcc
    30001 acgtaaaggt cctgttgcaa aacgtgatgt tttaccagat cctatttata actcaaaatt
    30061 agtaactcgc ttgatcaacc gtgtaatggt tgacggaaaa cgcgggattg cagctaacat
    30121 tatctataat gcctttgact tgatcaaaga atctacaggt aacgatccat tagaagtgtt
    30181 tgaacaagca atgaaaaacg ttcaacctgt tcttgaagtt aaagcacgtc gtgttggggg
    30241 ttcaaactac caagtaccag ttgaagttcg ccctgaacgc cgcgttactc taggtcttcg
    30301 ttgggtagta aactacgctc gcttgcgcgg tgaacacaca atggaagaac gcttagcaaa
    30361 agaaatcatg gatgctgcaa acaacacagg tgcttctgtt aaaaaacgtg aagacactca
    30421 taaaatggct gatgccaacc gtgcgtttgc tcactatcgc tggtaagatt ctctctgttt
    30481 ggtcggcttt tataagtcga cctcagatag tgtccttatt tttagcaact agatagtaaa
    30541 gagaggagaa acaaggagaa tgaccagaga attttcttta caaaacactc gtaatatcgg
    30601 tatcatggcc cacatcgatg ctggtaaaac gactgctact gaacgtatct tgtactatac
    30661 tggtaaaatc cataaaattg gtgaaactca tgaaggagct tcacaaatgg actggatgga
    30721 acaagaacaa gaacgtggta ttacgatcac ttctgctgct actacagcac aatggaaggg
    30781 tcaccgaatc aacatcatcg acacaccagg acacgtagac ttcacagttg aagttgaacg
    30841 ttcattgcgt gtacttgatg gtgctgtaac tgttcttgat gcacaatcag gtgttgaacc
    30901 tcaaactgaa acagtttggc gtcaagcaac aacttatggt gtacctcgta tcgtatttgc
    30961 aaacaaaatg gacaagatcg gtgctgactt cttatattca gtatcaactt tacatgaccg
    31021 tttgcaagca aacgctcacc caatccaatt accaattggt gcggaagacg acttcacagg
    31081 aattatcgac cttgtaacaa tgaaggctga aatgtataca aacgatcttg gaacagagat
    31141 cgaagaaaca gaaattcctg aagaataccg cgaattagct gaagaatggc gcgaaaaatt
    31201 ggttgaagct gtagctgaaa ctgatgaaga acttactttg aaatacttag aaggtgaaga
    31261 aatcactgaa gctgagttaa aagaaggtat ccgccgcgca acagttaacg tagaattcta
    31321 cccagtacta tgtggttcag catttaagaa caaaggggtt caattactat tggacgctgt
    31381 tcttgattac ttgccatcac cgttagatat cccagctatc aaagggattg accctaaaac
    31441 tgatgaagaa gtagaacgtc ctgcagacga cagcgctcct ttctcagctt tagcatttaa
    31501 agttatgact gacccattcg taggtcgttt aactttcttc cgtgtgtact ctggtgtttt
    31561 acaatcaggt tcatacgtac aaaacgcaac aaaaggcaaa cgtgaacgtg ttggtcgtat
    31621 cctacagatg catgccaact ctcgttcaga aatctctgaa gtttacgctg gagatatcgc
    31681 tgctgctgtt ggtttgaaag acacaactac tggggacaca ctatgtgatg aaaagagtct
    31741 agttattctt gaatccatgg aattcccaga accagttatc caagtcgcta ttgaacctaa
    31801 atcaaaagct gaccaagata aaatgggtgt ggctttacaa aaactatctg aagaagatcc
    31861 tactttccgt gctgaaacaa acgtggaaac aggcgaaaca atcatcgctg gtatgggtga
    31921 acttcactta gatatcatcg ttgaccgtat gcgtcgtgaa ttcaaagttg aagctaacgt
    31981 aggtgcgcct caagtatctt accgtgaaac tttccgtgct ggcactcaag ccgaaggtaa
    32041 gtttgtacgt cagtctggtg gtaaaggtca atacggtcac gtatggattg aatttacacc
    32101 aaatgaagaa ggtgctggtt tcgaatttga aaatgcgatc gtcggtgggg ttgttcctcg
    32161 tgaatacatt ccagctgttg aaactggttt gaaagatgct atggaaaatg gtgtcttagc
    32221 tggctatcca ttagtagata tcaaagctaa actttacgat ggttcatacc atgatgtcga
    32281 ttcaaatgaa actgccttcc gtgttgctgc ttctatggca ttacgtgcag cagctaagaa
    32341 agctaatcca gtaatccttg aaccaatcat ggctgtagaa gttgttatcc ctgaagatta
    32401 tttaggtgat gttatgggtc acgtaactgc tcgtcgtgga cgtgttgaag gtatggaagc
    32461 tcgcgctggc ggccaacaag ttgttcgtgc gatggttcca cttgctgaaa tgtttggtta
    32521 tgctacaact cttcgttctg ctactcaagg tcgtggtaca tttacaatga catttgacca
    32581 ctacgaagat gtaccaaaat cagtacaaga agaaatcatt aagaaaaacg gcggaaaagc
    32641 tgaataattt cttgaaaccg gatagtcctg caaagagatt atccaaagag actatatttt
    32701 taatatagtt tcgtgtaaaa taattatcga tgatatgggc atgtggtgta ccttgtgtac
    32761 atcatatacc tatcagacaa ataaaaaata tattgtattt ttaggaggac attttaaaat
    32821 ggcaaaagaa aaatttgacc gttctaaacc acacgttaac attggtacta tcggacacgt
    32881 tgaccatggt aaaactacat taacagctgc aatcacaact gtactatcta agaaaaacgg
    32941 cggccaagct atggcttacg atcaaatcga tggtgctcca gaagaacgcg aacgcggaat
    33001 cacaatctca acagctcacg ttgagtatga aacagataca cgccactacg ctcacgttga
    33061 ctgcccagga cacgcggatt acgttaaaaa catgatcact ggtgctgctc aaatggacgg
    33121 agctatcttg gtagtttctg ctgctgacgg cccaatgcct caaactcgtg aacacatcct
    33181 attgtctcgt caagttggtg ttccttacat cgttgtattc ttgaacaaag tagacatggt
    33241 tgatgacgaa gaattactag aattagttga aatggaagtt cgtgacctat taacagaata
    33301 cgaattccct ggtgacgatg ttcctgtagt tgctggatca gctttgaaag ctctagaagg
    33361 cgacgcttca tacgaagaaa aaattcttga attaatggct gcagttgacg aatacatccc
    33421 aactccagaa cgtgacaacg acaaaccatt catgatgcca gttgaagacg tgttctcaat
    33481 tactggacgt ggtactgttg ctacaggtcg tgttgaacgt ggacaagttc gcgttggtga
    33541 cgaagttgaa gttgttggta ttgctgaaga aacttcaaaa acaacagtta ctggtgttga
    33601 aatgttccgt aaattgttag actacgctga agctggagac aacattggtg ctttactacg
    33661 tggtgttgca cgtgaagaca tccaacgtgg acaagtttta gctaaaccag gtacaatcac
    33721 acctcataca aaattctctg cagaagtata cgtgttgaca aaagaagaag gtggacgtca
    33781 tactccattc ttcactaact accgtccaca attctacttc cgtacaactg acgtaacagg
    33841 tgttgttgaa ttaccagaag gaactgaaat ggttatgcct ggcgacaacg taacaatgga
    33901 agttgaatta atccacccaa tcgctatcga aaacggtact aaattctcaa tccgtgaagg
    33961 cggacgtaca gttggtgccg gtgtcgttac agaaatcaaa gcttaattca cttaagtttt
    34021 aaagtaaagc aacatttatt cggacgtaat cgctcggaac gaaggtctta ggatctttgt
    34081 tccgagcttt tttatttatt gcaggatcag gaaaaaagac aggagtatta taaatagaag
    34141 aaacacgaaa taaaagagtc agtattttct aaatgaaact ttatgaaaac aaaatgtttt
    34201 ttacaagaaa aagccttgct ttgtgagttt atatatatta taatgacaaa ggtgcgttta
    34261 ctttagtgaa ttgcacagac gtaatcagaa tgatttcgtc ggctaagaaa cgagaggttg
    34321 cgacacgccc ggacgctttg ccacgaacga gcgtgacgga aattttcgtg gagctatgtc
    34381 tacttttcaa aataggcgaa ggagggaaca agatggcaaa acaaaaaatt cgtatccgtt
    34441 taaaagcgta tgaacaccgt attttagatc aatcagcgga taaaatcgtg gaaactgcaa
    34501 aaagaactgg agctagcgtg tctggtccaa ttccattacc aactgaacgc agtctttaca
    34561 caatcatccg tgcgactcat aaatacaaag attcacgcga acaattcgaa atgcgtacac
    34621 acaaacgtct tatcgacatt gtgaacccaa caccaaaaac tgttgatgct ttgatgaagc
    34681 tagacttacc atctggtgta aacatcgaaa tcaaattata aaactaaaac aaaaataatg
    34741 gaggtgtact catgaccaaa ggaatcttag ggaaaaaagt gggaatgaca caaatcttca
    34801 ctgaatctgg tgaattaatt ccagtaactg ttgtagaagc tactccaaac gtagtactac
    34861 aagtgaaaac aatggaaacg gatggctacg aagctattca agttggttac caagatatgc
    34921 gtgaagtttt atcaaacaaa cctgcgaaag gtcatgttgc aaaagcaaac acggctccta
    34981 agcgcttcat tcgtgaattc aaaaatgttg agctaggaga atatgaagta ggaaaagaaa
    35041 ttacagtaga tgttttccaa gcaggagaca ttattgatgt aacaggtact acgaaaggta
    35101 aaggattcca aggggttatc aaacgccacg gacaaagccg cggaccaatg gctcacggtt
    35161 ctcgttacca tcgtcgtcct gggtcaatgg gtccagttgc acctaaccgt gtatttaaaa
    35221 acaaacgtct tgctggccgt atgggtggca accgcgtaac tatccaaaac ttggaagttg
    35281 tacgtgtcga tgcagaaaga aatgttatct tgatcaaagg taatgttcct ggagctaaaa
    35341 aatctttaat tactatcaaa tcagctgtga aagctaaata attgtaggaa gaaaggagga
    35401 actaaggaat gccgaatgta gcattattca aacaagatgg aagccaaaac ggtgaaatca
    35461 cacttaacga agaaatcttc ggaattgaac ctaatgaaag tgttgtttac gatgcaatcg
    35521 taatgcaacg tgcgtcatta agacaaggaa cacacgcggt taaaaaccgt agcgctgttc
    35581 gcggcggcgg acgtaaacca tggcgtcaaa aaggaacagg tcgtgcacgt caaggatcta
    35641 tccgctcacc acaatggcgt ggaggtggcg tagtctttgg accaacacca cgttcttaca
    35701 gctacaaact tcctaaaaaa gttcgtcgtt tagcaatgaa atctgtattg tcagaaaaag
    35761 ttgctgaaaa caacttggta gcaatcgaag gcttgaactt cgacgcacca aaaacaaaag
    35821 aattcaaaca agttcttgct aacttatcta tcgactctaa agtattggtt gttctagaaa
    35881 aaggtaacga ctttgctgct ttatcagcac gtaacttacc aaacgtttct gtagtaactt
    35941 ctgataatgt gagtgtgcta gacgttgtat caaatacaaa agtgttagca acacaaactg
    36001 ctcttactca aattgaggag gtgcttgcat aatgaactta ctagacgtaa tcaaacgccc
    36061 agtgatcact gaaaaatcaa tgcttgccat ggatgacaag aaatacactt ttgaagtgga
    36121 cactcgcgca aacaaaactt tagtaaaaca agcggtagaa gctgcttttg acgttaaagt
    36181 caaaaacgtg aacatcgtga acgtacgtgc gaaattcaaa cgtatgggca aatatgcagg
    36241 atatacaaaa aaacgtcgca aagcgatcgt tacattgact gaagattcaa aagaaatcca
    36301 attattcgaa gctgctgaat aagcactaat agataactca ttaaattagg agggaaaaac
    36361 gtggcgatta aaaagtacaa acctacctca aatggtcgtc gtaacatgac aggttctgat
    36421 tttgctgaaa tcacaacatc aacacctgaa aaatcattgt tacaaccatt gaaaaaccat
    36481 gctggacgta acaacaacgg ccgcatcact gtacgtcatc aaggtggcgg acacaaacgc
    36541 caatatcgcg tgatcgactt caaacgtaac aaagataacg ttgttgcaac tgttcaaacg
    36601 atcgaatatg atccaaaccg ttcagctaac atcgcgttag tacactatga agatggagtg
    36661 aaagcttaca tcttagcacc taaaggacta gaagtgggta tgaaacttgt ttcaggtcca
    36721 gaagcagata tcaaagtcgg aaatgctctt ccattagaaa acattccagt tggtactgtt
    36781 atccacaaca ttgaaatgaa acctggtaaa ggtggacaat tgatccgttc tgctggtaca
    36841 agtgctcaag tacttggtaa agaaggcaaa tacgtattga tccgcttgaa ctctggtgaa
    36901 gttcgtatga tcttagctgc atgccgtgct acaatcggtt ctgtaggtaa cgaacaacac
    36961 gaattaatca acatcggtaa agctggacgc tctcgttgga tgcgtaaacg cccaactgta
    37021 cgtggtagcg taatgaaccc taatgatcac ccacacggtg gtggtgaagg taaagctcct
    37081 atcggacgta aagcaccagt ttctccttgg ggtcaaccag ctcttggtta caaaactcgt
    37141 aacaaaaaag ctaaatcaga caaacttatt gttcgtcgtc gcaaaaataa ataattactt
    37201 tgaccatgtg tcgcacattt tgagaggagg ttcaccatgg gtcgtagttt aaagaaagga
    37261 ccttttgtcg atgagcattt aatgaaaaaa gttgaagctc aagcaggggt tgaaaagaaa
    37321 aaagttatca aaacttggtc acgccgttca acagttttcc caagttttgt tggatatacc
    37381 atcgcagttt atgatggacg taaacatgta ccagtttaca tccaagaaga catggtagga
    37441 cacaaattag gtgaatttgc acctacaaga acttatcgtg gacatgctgc tgacgataag
    37501 aaaacgaaac gctaatttga ggggaggata aatccaaatg gcagaacaaa tcacatcagc
    37561 taaagcaact gcaaaaacag ttcgcacttc acctcgaaaa gcccgtttag taattgacct
    37621 tatcagaggt aaaagtgtcg cagatgcaat ttcaatcttg aaattcacac caaacaaatc
    37681 tgctggaatc attgaaaaag tattgatgtc agcagttgcg aatgcagaaa ataactttga
    37741 cttagatgtt gaaaacttgg tagtaagcga agcttttgtt aacgaaggac ctacaatgaa
    37801 acgtttccgt cctcgtgcaa aaggatcagc ttctccaatc aacaaacgta caagtcatat
    37861 cacggtagtc gtatcagaaa aataaggagg gataatcagt gggtcaaaaa gtacatccaa
    37921 ttggaatgcg tgtaggcatc atccgcgact gggatgctaa atggtatgct gaaaaagagt
    37981 atgctgagtt cttacacgaa gatttaagaa tccgtaaatt tatttcaact aaacttgctg
    38041 atgctgctgt atcaacagtt gaaatcgaac gcgcaacagg tcgcgtaaac atttcaattc
    38101 acacagctaa accaggtatg gttattggta aaggcggttc tgaagtcgaa agcctaagaa
    38161 aagaattaaa caaattaact ggtaaaagaa tccatatcaa tatcgtggaa atcaaaaaac
    38221 cagatctaga tgcaaaatta gtaggtgaag gaatcgcacg tcaattagaa aaccgtgttg
    38281 ctttccgtcg cgctcaaaaa caagccatcc aacgctcaat gcgtgctggc gctcaaggga
    38341 tcaaaactca agtatctggt cgtctaaacg gagcagatat cgctcgttca gaaggatact
    38401 cagaaggaac tgttcctctt catactttac gtgcggacat tgattacgca tgggaagaag
    38461 cagatacaac atacggaaaa ctaggagtta aagtgtggat ctatcgtgga gaaattcttc
    38521 caactaaaaa aaacactgag aaaggaggga aataatcatg ttagtaccta aacgtgtaaa
    38581 acaccgtcgt gaattccgcg gaaaaatgcg cggggaagct aaaggcggaa aagaagtagc
    38641 attcggtgaa tacggtttgc aagctgttga ttcacattgg atcacaaacc gccaaatcga
    38701 agctgctcgt atcgcaatga ctcgttacat gaaacgtggt gggaaagtat ggattaaaat
    38761 tttccctcac aaatcttata ctgccaaagc aattggggta cgtatgggtt ctggtaaagg
    38821 ggcacctgaa ggatgggttg caccagtaaa acgtggtaaa atcatgtttg aaatcgcagg
    38881 cgttcctgaa gaagtagctc gtgaagcgtt acgtctagct tctcacaaat taccaatgaa
    38941 aactaagatc gtaaaacgtg aggaaatggg tggtgaatcg aatgaaggtt aaagaaatca
    39001 gagaattaac cactgccgaa atgcttgatc aagaaaaaca attaaaagaa gaattgttta
    39061 atcttagatt ccaattagca acaggtcaat tagaaaacac tgcacgtatt aaagaagtac
    39121 gtaaatcgat tgcacgcatc aaaactgtgt tgcgtgaaca agctaagtaa ttatggaagg
    39181 aggccattaa gcgtatgact gaaggaagaa atcaacgcaa agtttatcaa ggtcgcgtgg
    39241 tttcagacaa aatggataaa acaattaccg ttgctgtcga aacaaagaaa aaccacccta
    39301 tctatggcaa acgcatgaac tattctaaga aatacaaagt gcatgatgaa aacaacacag
    39361 caaaagttgg cgacatcgtg agaattatgg aaactcgtcc attatcagct acaaaacgtt
    39421 tccgtttgtt ggaagtagtc gaagaagcag ttattatcta ataacacgag attttcctat
    39481 atagattgaa tgcaaaatct gaaaggagga tacacaacgt gatccaagca gaaagtcgtt
    39541 taaaagtcgc tgataactca ggcgcacgtg aaattttgac gatcaaagtc cttggtggat
    39601 ctggtcgtaa aactgctaac atcggtgatg tgatcgttgc tacggttaaa caagcaacgc
    39661 caggtggggt tgtcaaaaaa ggtgacgtcg ttaaagccgt tatcgttcga acaaaatcag
    39721 gagctcgtcg tacagacggt tcatatatta aatttgatga aaatgctgca gtgattattc
    39781 gtgatgataa aagccctcgt ggaacacgta tctttgggcc agttgcacgt gaactacgtg
    39841 aaaacaactt catgaagatc gtttcactag caccagaagt tttataatct tgagacactt
    39901 atcaaaggag gtgcgaaaca gttatgtttg ttaaaaaagg cgataaagtt aaagttatca
    39961 ctggaaaaga caaaaacaaa gaaggcgttg tactagcagc gttcccgaaa aaagataaag
    40021 ttattgtcga aggtgtaaat gtcgttaaaa aacaccaaaa accttctcaa gctgctcctc
    40081 aaggtggaat tgttgagatg gaagcgccaa ttcatgtttc taacgtgatg gtggttgact
    40141 ctactggtgt agctggccga gttggctaca aagaagtaga cggcaaaaaa gtccgtgttt
    40201 ctaaaaaaac cggtgaagtt ttagataaat aaaagtttaa ggaaggaggg tcttactgaa
    40261 tgaaccgcct aaaagaaaaa tatcttaaag aagtaactcc atcattgatg gaaaaattta
    40321 actatagctc tgttatgcaa acacctaaag ttgaaaaaat cgtcatcaac atgggtgttg
    40381 gtgatgcggt atcaaacgct aaaaacttag ataaagcagt tgaagaatta actttgatca
    40441 ctggtcaaaa accaatgatt acaaaagcta aaaaatcaat cgctggattc cgtttacgtg
    40501 aaggaatgcc aattggtgca aaagttacat tgcgcggaga aagaatgtac gaattcttag
    40561 acaaattggt ttctgtttca ctaccacgtg tacgtgactt ccatggtgta agtaaaaaag
    40621 cctttgacgg tcgtggaaac tatactttag gtattaaaga acaattgatc ttcccagaag
    40681 tagattatga tttagtagat aaagtacgtg gtatggacat cgttattgta acaacagcaa
    40741 actctgatga agaatctcgt gagttgttga cacaattagg catgccattc caaaaataat
    40801 taaaggaggc gaactacgtg gctaaaaaat caatgattgc taaaaacaaa cgtcctgcaa
    40861 aattttcaac acaagcttat actcgttgcg aacgttgcgg acgtccacat tcagtttatc
    40921 gtaagttcca tctttgccgt atttgcttcc gcgaacttgc ctataaaggt caaattcccg
    40981 gcgtgaagaa agctagctgg taaaaagtaa attcgcataa gaaggaggta aaagatcaat
    41041 ggtcatgaca gatccaattg cagattttct aactcgtatc cgtaacgcaa acatggttaa
    41101 acatgagtct ttagaagtac ctgcatcaaa aattaaacgt gacatcgctg aaatcttgaa
    41161 acgtgaaggt ttcgttcgtg atgttgaata catcgaagat gacaaacaag gcgtgatccg
    41221 tgttttccta aaatttggta aaaacggcga acgtgttatc acaaacttaa aacgtatctc
    41281 taaaccaggt ttgcgtgctt atgtcaaagc tgacgaagta ccaaaagtat tgaacggttt
    41341 aggtatcgca atcatttcga cttctgaagg tgttgtcact gataaagaag ctcgtgaaaa
    41401 aaatatcggc ggcgaagtta tcgcttacgt atggtaatca agtagaatct acaaggaggt
    41461 gtctttaagt gagccgtatc ggaaataaag tcgttgttat ccctgaagga gtaacagtta
    41521 ctcaagacgg aaacaacgtt acagttaaag gaccaaaagg tgaattgact cgtacttttt
    41581 cagcagatat caaaatgaat atcgaaggaa acgaagtaac tttcactcgt ccaaacgaca
    41641 gtaaagaaat gaaaacaatt catggaacaa ctcgtgcgaa cttcaacaac atggttgtag
    41701 gtgtcagcga aggtttccaa aaagcgttag aactaatcgg ggttgggtac cgtgcacaat
    41761 tacaaggatc aaaacttgta ttgaacgttg gttactcaca tccagtagaa atcacaccac
    41821 cagctggagt aactgttgaa gtaccagcta acacacaagt aattgttaaa ggtgcgaaca
    41881 aagaagaagt tggcgaatta gctgcaaaca ttcgtggaac tcgtcctcca gaaccttata
    41941 aaggtaaagg tattcgttat gttggcgaat atgtacgccg taaagaaggt aaaactggta
    42001 aataatcgtt tagaaaaatt gcatagaagc cagcagcagg aaaaaggcat ttggctgaag
    42061 tttaccttca aaaaaataca tttttacttg tgccgcagct tctatttttc gaaacgtatt
    42121 tactgcttga aatagtagta caataaaaat aaagaggtga caattgtgat tacaaaacca
    42181 gataagaaca aaacacgtca aaagagacat cgtcgtgtac gtaacaatat ctctggtact
    42241 gctgagcgcc cacgcttgaa cgtttttcgt tctaacaaaa acatctacgc tcaaattatt
    42301 gatgacgtag cgggtgtaac gctagcaagt gcctctacct tggataaaga aatttcaggt
    42361 ggaacaaaaa cagaagcagc acaagctgtc ggtaaactag ttgctgaacg tgcagctgaa
    42421 aaaggcatta aagtagtagt ctttgaccgt ggtggatacc tttaccatgg ccgtgtgcaa
    42481 gctttagctg aagctgctcg cgaaaatgga ctagaatttt agaagaagga ggaataccat
    42541 tcatggttta tatcgatcca aaacatttgg aattagaaga ccgcgttgtt gcaattaacc
    42601 gtgtaacaaa agttgttaaa ggtggacgtc gtctacgttt tgctgcttta gttgttgtcg
    42661 gtgacaaaaa cggacacgta ggatttggta caggtaaagc tcaagaagtt cctgaagcaa
    42721 tccgtaaagc aattgaagat gcgaagaaaa acttagttga agtacctatg gttggttcaa
    42781 caatcccaca cgaagtaatc ggtgctttta gtggtggtag aatcctaatg aaacctgctg
    42841 tagaaggttc tggggttgct gctggtggac cagttcgtgc cgtattagaa ttagctgggg
    42901 tagcagatat cacttctaaa tctttaggct caaatacacc aatcaacgtt gttcgcgcaa
    42961 cagttgaagg tttgaaacaa ttgaaacgtg cagaagaagt tgcagaactt cgcggcaaat
    43021 cagttgaaga attaatcgga taaggaggac aacaataatg gctgaattaa aagttacttt
    43081 aaaacgcagt attatcggac gtcctcaaaa ccaacgcgat acagttaaag cgttaggtct
    43141 tacaaagatt aacagttcag tggttaagcc ttctaatgaa gcaattaaag gcatgatcaa
    43201 tactgtttct cacctagtgg acgttgaaga aatttaattt agaaaaatca taactagaac
    43261 attaaggagg tgccaaacct atgaaacttc atgaattaaa acctgctgaa ggttcacgcc
    43321 aagtacgtaa ccgtgttggt cgtggtactt catctggtaa cggaaaaact gccggacgcg
    43381 gacaaaaagg acaaaaagct cgttcaggcg gcggtgtgcg tttaggattt gaagggggac
    43441 aaacaccatt gttccgtcgt ttaccaaaac gaggattcac aaacgtgaac cgtaaagact
    43501 atgcagtcat taatcttgat gtattgaacc gctttgaaga cggagctgaa gtaactcctg
    43561 ttgctttagt tgaagctgga atcgtgaaaa acgaaaaagc tggaatcaaa gtattagcta
    43621 atggggaact aaacaagaaa ttaactgtga aagcagcaaa attctctaaa gcagctcaag
    43681 aagcaattga agcggctggt ggatcaatcg aggtgatcta atgtttagac tattaaaaga
    43741 tgcttttaaa gtaaaagaca ttagatcgaa aatcctattt actgtatttg tgttgtttgt
    43801 atttcgccta ggtgcacata ttactgtacc aggcgtaaat gctgcttcat tgcaaaacct
    43861 ttcttctttg ccatttttca acatgttgaa cttggtaagt ggtagtgcga tgcaaaactt
    43921 ttccattttc tcaatggggg tttcaccata cattactgct tctatcgtca ttcagcttct
    43981 tcaaatggac atcgtgcctc gatttgttga atggtcgaaa caaggggaag ttggtcgtaa
    44041 aaaactgaat caagcgactc gatatctgac attagtattg gcatttatcc aatctgtcgg
    44101 tatcactgct ggattcaatc aatacgctca attaggtttc gtgaataatc ctaacgtagc
    44161 aacttacgta acgatcggtt tgattctaac agccggtacg atgtttgtta cttggctagg
    44221 agaacagatc acagaaaaag gtatcggaaa tggcgtatca atgatcattt ttgcaggtat
    44281 catttctaga ttgcctgaaa gtatcagaga aatttatgaa gattacttta taaatattga
    44341 tccttcagac atatggaaat ctgctatttt tgtggccatt ttactagtag cggtcttgtt
    44401 gatcgtgaca ttcgtaacat ttttccaaca agctgaacgc aagatcccga tccaatatac
    44461 gaaacgcgta gctggcgcgc ctacaagcag ctatctgccg ttgaaagtaa atgcagctgg
    44521 agttattcca gttatctttg ctagttcgtt tattgctacg ccaaatgctg ttttacaagc
    44581 attgagcggt agattcggtt ctgaaaactg gtacaaagtg atgatggaga tctttaacta
    44641 taatacagca cctggtgcta cgatttatac gattctgatc gttgccttca ctttcttcta
    44701 cgcctttgtt caagtaaacc ctgagaaatt agcggaaaac ttgcaaaagc aaggaagtta
    44761 tataccaagc gtacgccctg gtaaggggac tgaagagtat gtctcaagat tactgatgcg
    44821 tttaagtaca gttggtgcag tcttccttgg tttagtggca ttgcttccaa tcattgctca
    44881 aatggtttgg gatcttccac aatcaatcgg attaggcggt acaagcttac tgatcgttat
    44941 cggtgtagct ttagaaactg cgaaacagtt agaaggctta atgctgaaac gtaaatatgt
    45001 tggattcatt gagtaagaaa gcgtgttgag gtttcccctc aacacacgtt ctcacacatt
    45061 tattaggagg gaaaacaatg aacctcattt taatggggct gccaggcgca ggtaaaggaa
    45121 cacaagctga aaaaatcatt gatgcttatg gtattccgca catctcaaca ggagatatgt
    45181 tccgcgcagc gatgcaaaac gaaacagctc ttggcctgga agcaaagtct tatatggata
    45241 aaggtgcatt agttcctgat gaagtaacaa atggaatcgt gaaagagcga ctagctgaac
    45301 cagatacaga gaaaggcttc ttattagatg gctttccacg tacgctagat caagcaaaag
    45361 ctctggacgc aatgctaaaa gatttgaata aaaaaattga tgctgtcatc gatatccatg
    45421 ttggtgaaga aatcttggtc gaacgtctag ccggccgatt tatctgcagc aattgtggag
    45481 caacttacca taaaattttc aaccctacaa aagttgaaga cacatgtgat cgttgcggtg
    45541 ggcatgaatt ctatcaaaga gaagatgata aacctgagac ggttaaaaat cgtctggcta
    45601 tcaacatcaa aaatagtgaa ccaatcttgg cttattataa agaacaaggt ttgctaaaca
    45661 ctattgatgg tgctcgtgaa atcgatgctg tatttgcaga tgtacaaaaa atcattgaaa
    45721 aatagcacga ttgcccgtag ccgacttgca ttatgcagtt actcttgtca agttcaataa
    45781 attatgatag aatatgtcag tccgacttct agagaatcct ctagatgatc cattttgaga
    45841 attttgaggt gggaactgca gtttccgcca ttttatcgtg tgaaaatgcg aaacaagtac
    45901 aaggaggtac tgtgcgtggc aaaagaagat atgattgaag tcgaaggtac agtcgtcgaa
    45961 actttgccga atgcaatgtt taaagttgaa ctagaaaacg gacaccaagt tcttgctact
    46021 gtttcaggta aaattcgtat gcattacatc cgtatcttac ctggtgacaa agtaacggtt
    46081 gaattgtctc catacgatct taaccgtggc cgtattactt atcgctttaa ataattgtac
    46141 tccgtaaaac tacaggaggt attatcatga aagtaagacc atcagtaaaa ccaatgtgcg
    46201 aacattgtaa agtaattcgt cgtaaaggac gcgttatggt gatttgccca gcaaatccaa
    46261 aacataaaca acgtcaagga taattggagg tgctataaat atggctcgta ttgcaggagt
    46321 agatattcct cgtgataaac gtgtagtagt ttcacttact tatatctatg gtattggtaa
    46381 cactactgct aaaaagatct tagctgatgc tggtgtatct gaagatgttc gcgttcgtga
    46441 attaacgaat gaacaaacag atgctatccg tgcagaagta gataaattaa aagttgaagg
    46501 ggatcttcgt cgtgaagtga acttgaacat caaacgtttg atggaaatcg gttcttaccg
    46561 tggaatccgt catcgccgtg gattgcctgt tcgtggacaa aacacgaaaa acaatgcacg
    46621 tactcgtaaa ggcccagctc gcgctatcgc tggcaagaaa aaataattct taagtgaagg
    46681 aggttaaaac ttcatggcag caaaaaaagt gaatcgtaaa cgccgtgtga agaagaatat
    46741 agaagcaggt attgcacata tccattctac attcaataac actatcatca tgatcactga
    46801 tactcacgga aatgcattag cgtggtcatc agctggttca ttaggtttca aaggaagcaa
    46861 aaaatcaact ccttttgccg ctcaaatggc agcagaaact gctacaaaag cagcaatgga
    46921 acacggatta aagactgttg aagtaacagt taaaggacct ggttctggac gtgaagcagc
    46981 aattcgttca ttacaagcta caggtttaga agtgactgca attcgtgacg tgactccagt
    47041 tcctcataat ggtgcccgcc ctccaaaacg ccgtcgtgtt taatgagtgc cgctcatttt
    47101 gagttttatc agtaacgtca ttgaacaaga cacttttcgt tttgaaaggg gtaaagataa
    47161 gaatgattga gttcgaaaaa ccaagaatcg caaaaattga tgaagaaaaa gattatggca
    47221 agttcatcgt tgaacctctt gaaagagggt atgggactac tttagggaat tccctacgtc
    47281 gtattttatt atcttctttg ccaggtgctg ccatcactaa tattcaaatc gatggtgtac
    47341 tgcatgaatt ctctaccatc aaaggtgtta gagaagacgt cactcaaatc atcttgaaca
    47401 ttaaaggctt agcgctgaaa atgtatggcc aagaagaaaa aacccttgaa atcgatatta
    47461 ccggacctgc tacagtaact gctggcgata ttatcgttga tagtgatgta gaaattctta
    47521 acaaagatat gtacatctgt agtgtctctg aaggagctac gttccatgct cgcttaacag
    47581 tgaaacctgg acgtggttat gttcaagcag atgaaaataa aaaagaagat atgccaattg
    47641 gcgttcttcc agtcgattca atctacacac ctgttcgtcg tgtgaactat caagttgaga
    47701 acacacgtgt tggacaccgt gaagattttg ataaattgac gatggagatt tggacagatg
    47761 gttcaattga accgttggaa gcaatgagtt tgtctgcgaa aatcatgact gaacatttgg
    47821 atatttttgt gaacttaact gatgaagcaa aaaatgctga aatcatggtc gaaaaagaag
    47881 aaacacaaaa agaaaaaatg ttggaaatga cgatcgaaga gttagacttg tctgttcgct
    47941 cttacaattg tttgaaacgt gcaggaatca atactgtaca agaacttaca aataaatctg
    48001 agcctgaaat gatcaaagtc cgtaacttag gacgtaaatc attagaagaa gttaaagcaa
    48061 aattgcacga cttaggttta ggtttacgta aagaagatta aaacctttta cgaaggaggg
    48121 aaaccaacgt gagttatcgt aaactaggac gcacatctag ccaacgtaaa gcgatgttgc
    48181 gtgatttaac aactgattta ttaatcaacg aacgtattgt gactactgaa gctcgtgcga
    48241 aagaagttcg ctcaactgca gaaaaaatga tcactttagg taaacgcgga gatttgcatg
    48301 cacgtcgtca agctgcggct ttcgtacgta atgaagttgc cagcgtgcgt gaagaaaatg
    48361 aggacatcgt cattgaatca gctttacaaa aactgttcaa cgatattgca cctcgttacg
    48421 cagaacgtca aggcggatac acacgcattt tgaagacaga accaagacgc ggtgatggtg
    48481 caccaatggt tgtcttagaa cttgtctaaa atcacactaa taacatcact ttatagatga
    48541 ttcttttaga gtgttatgat gttggaagat atttttccga gtctagctca gcgctgctcc
    48601 tcaagcgaat atcgcttggg gagtaagcac tcatcataaa gtgttttttt ataccttcaa
    48661 ttacctgtca tgacagatat gtttgaaggc ataaaaaaac attttaagcg agaaataatt
    48721 gaaaattttg tctttttttg atagtgttag taggaacttt aggatgaaga aggagtaatc
    48781 atggagccaa ttattgaatt agaaaagata aattataaat accagccaga tgatcttcgc
    48841 cccgcattga aagatgtctc ctttacaatt gataaaggtg agtggatcgc tattattgga
    48901 cacaatggtt caggaaaatc gacacttgcc aaaacgatca atggattgct tctgccagaa
    48961 tcagggatcg tgaaagtggg aaatcaaata ttggatgaag aaaatatttg gacgatccga
    49021 cagatggttg gaatggtttt tcaaaatcca gacaatcagt ttgttggttc gacagtagaa
    49081 gatgacgttg cttttggtct tgaaaatcaa gggattccca gagaagaaat gctagtgcgt
    49141 gtgaaagatg cacttgagaa agttagaatg gctgagtttg cttcacgcga gcctgctcgt
    49201 ttatcaggag ggcaaaaaca acgtgttgcc attgccggcg tggttgcctt acgacctgat
    49261 atcattattt tagatgaagc aaccagtatg ctagatccag aaggtcgtga agaagtgatc
    49321 tctacgatca agaagatcaa agaagaaagt caattgaccg ttatatctat cactcatgat
    49381 attgatgaag cagctaatgc caatcggata ctagtgatga gacaaggcga acttgtccgt
    49441 gaaggtacac caaaagaaat tttttctgct ggtcctgaac tgatcgactt aggtctagat
    49501 ctaccatttc ctgaaaaact gaagagcgca ctgaaagaac gtggggttga tgtgcccagt
    49561 gaatatatga cagaagaaag gatggtggat tggttatgga catccgtttt gaacaagtag
    49621 acttcactta tcagccgaat acaccatttg aacaaagagc cttatttgac atcaatatga
    49681 cgatcaaaga aaacagttat actgcattag tagggcatac cggaagcggg aaatcaacct
    49741 tacttcagca tttgaatgcg cttgttaagc caacaagcgg tacagtccat attggagaac
    49801 gagatattca gccggatacg gataataaga atttaaagcc catccgaaaa aaagttggca
    49861 tcgtctttca gtttccagaa gcacagctat tcgaagaaac ggtagcaaaa gacattgctt
    49921 tcggtcctaa aaactttggt gtcagcgaag aagaagcact agtcctagca aaagaaacat
    49981 tagaacaagt tgggctggat gaaagctatt tggaacgttc gccatttgaa ctttcaggag
    50041 ggcaaatgcg tagagtagca atcgctggtg tgcttgccat gaggccggaa gtacttgtat
    50101 tggatgaacc aacggcagga cttgatcccc agggcagaaa agaaatgatg gagatgtttt
    50161 ggcgtcttca taaggaacag cagatcacta ttgtattagt tacgcattta atggatgatg
    50221 tagccaattt tgcagattat gtctatgtgt tggaaaaggg acgaatcgtt aatagcgggg
    50281 agccgcaaga ggtctttcaa aacattgaat ggctaaaaga aaaacaacta ggcgtaccta
    50341 ctgccaccga gtttgcagaa gaattaatgg ctaaagggat gaactttgct accttgcctt
    50401 tgacagctga agaattagca gacgctatcg tccattatgc tggaggaaat gcccatgatg
    50461 aataaattga tatttggtcg ttatatgcct ggagattcct tgatccatcg attggatccc
    50521 cgagctaaat tattagccag tttttatttt atcggaatca tttttttagc aaataattgg
    50581 gaaacgtacc ttttgttggc atttttcaca ttactttctg tcttcttgtc aaaagtagat
    50641 ttgaaatttt ttatccgagg cattcgccca ttgatctggc taatcctctt cactgtagct
    50701 ttgcaggtct tgtttacaag tggcggagaa acttattgga gttggggtgt tttccatatc
    50761 actgaatttg gtgtgatgaa cggcctcttt attttctgtc gattcgtgtt gatcattttt
    50821 atgtcgactc tactcacgct tactacaccg cctttggaat tatcggatgc gattgagtac
    50881 ttgcttcgtc cattgaaagc cgtacggttc ccagtccacg aaatttcatt gatgctgtcg
    50941 attgctcttc gttttgtgcc tacattgatg gacgaaacag aaaagatcat gaacgctcag
    51001 cgcgctcgcg gtgttgattt tggtgaagga aacctagttc agaaaatgaa agcagtcatt
    51061 cctttgctga ttcctttatt tgtcagcagc ttcaaccgtg cggaagatct tgcgactgct
    51121 atggaagcaa gaggctacaa aggtggggaa ggaagaacga aatatcgtgt attgcattgg
    51181 cataatagag acacgatggt tatcattgct tttgtattat taactgtgct actgatcttt
    51241 ttaagaggat aaacgttcag tgggccgcta tctattcttt tcttgctgat ctgtttgcaa
    51301 gaaaggatgc aagcagtgta tgacaagaaa tattagggtc tgtggcagac taaggtcaca
    51361 ggcctctttc ttttatctga aaagaaagaa cgaaa
//
LOCUS       DAZKMB010000007        49977 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000007 DAZKMB010000000
VERSION     DAZKMB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.007"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..171)
                     /locus_tag="ACG8V3_000377"
     CDS             complement(<1..171)
                     /locus_tag="ACG8V3_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0298949.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNY"
     gene            complement(173..370)
                     /locus_tag="ACG8V3_000378"
     CDS             complement(173..370)
                     /locus_tag="ACG8V3_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0298950.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(764..2491)
                     /locus_tag="ACG8V3_000379"
     CDS             complement(764..2491)
                     /locus_tag="ACG8V3_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0298951.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2484..3677)
                     /locus_tag="ACG8V3_000380"
     CDS             complement(2484..3677)
                     /locus_tag="ACG8V3_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0298952.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3864..4508)
                     /locus_tag="ACG8V3_000381"
     CDS             complement(3864..4508)
                     /locus_tag="ACG8V3_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0298953.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4602..4736)
                     /locus_tag="ACG8V3_000382"
     CDS             complement(4602..4736)
                     /locus_tag="ACG8V3_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0298954.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4738..6870)
                     /gene="feoB"
                     /locus_tag="ACG8V3_000383"
     CDS             complement(4738..6870)
                     /gene="feoB"
                     /locus_tag="ACG8V3_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0298955.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6867..7340)
                     /locus_tag="ACG8V3_000384"
     CDS             complement(6867..7340)
                     /locus_tag="ACG8V3_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0298956.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7741..7965
                     /gene="nrdH"
                     /locus_tag="ACG8V3_000385"
     CDS             7741..7965
                     /gene="nrdH"
                     /locus_tag="ACG8V3_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0298957.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8124..10283
                     /gene="nrdE"
                     /locus_tag="ACG8V3_000386"
     CDS             8124..10283
                     /gene="nrdE"
                     /locus_tag="ACG8V3_000386"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0298958.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10333..11298
                     /gene="nrdF"
                     /locus_tag="ACG8V3_000387"
     CDS             10333..11298
                     /gene="nrdF"
                     /locus_tag="ACG8V3_000387"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0298959.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11387..12526)
                     /locus_tag="ACG8V3_000388"
     CDS             complement(11387..12526)
                     /locus_tag="ACG8V3_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0298960.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12835..13548)
                     /locus_tag="ACG8V3_000389"
     CDS             complement(12835..13548)
                     /locus_tag="ACG8V3_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0298961.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13802..14503)
                     /gene="nagB"
                     /locus_tag="ACG8V3_000390"
     CDS             complement(13802..14503)
                     /gene="nagB"
                     /locus_tag="ACG8V3_000390"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0298962.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14737..16269
                     /locus_tag="ACG8V3_000391"
     CDS             14737..16269
                     /locus_tag="ACG8V3_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0298963.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16460..17662)
                     /locus_tag="ACG8V3_000392"
     CDS             complement(16460..17662)
                     /locus_tag="ACG8V3_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0298964.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17744..18433)
                     /locus_tag="ACG8V3_000393"
     CDS             complement(17744..18433)
                     /locus_tag="ACG8V3_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0298965.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18539..18907)
                     /gene="rplL"
                     /locus_tag="ACG8V3_000394"
     CDS             complement(18539..18907)
                     /gene="rplL"
                     /locus_tag="ACG8V3_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0298966.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18960..19463)
                     /gene="rplJ"
                     /locus_tag="ACG8V3_000395"
     CDS             complement(18960..19463)
                     /gene="rplJ"
                     /locus_tag="ACG8V3_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0298967.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19667..19794)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19873..20562)
                     /gene="rplA"
                     /locus_tag="ACG8V3_000396"
     CDS             complement(19873..20562)
                     /gene="rplA"
                     /locus_tag="ACG8V3_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0298968.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20666..21088)
                     /gene="rplK"
                     /locus_tag="ACG8V3_000397"
     CDS             complement(20666..21088)
                     /gene="rplK"
                     /locus_tag="ACG8V3_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0298969.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21431..22099
                     /gene="sdaAB"
                     /locus_tag="ACG8V3_000398"
     CDS             21431..22099
                     /gene="sdaAB"
                     /locus_tag="ACG8V3_000398"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0298970.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22112..22984
                     /gene="sdaAA"
                     /locus_tag="ACG8V3_000399"
     CDS             22112..22984
                     /gene="sdaAA"
                     /locus_tag="ACG8V3_000399"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0298971.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23075..24175)
                     /locus_tag="ACG8V3_000400"
                     /pseudo
     CDS             complement(23075..24175)
                     /locus_tag="ACG8V3_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24267..25154)
                     /locus_tag="ACG8V3_000401"
     CDS             complement(24267..25154)
                     /locus_tag="ACG8V3_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0298972.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25231..25776)
                     /gene="nusG"
                     /locus_tag="ACG8V3_000402"
     CDS             complement(25231..25776)
                     /gene="nusG"
                     /locus_tag="ACG8V3_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0298973.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25877..26047)
                     /gene="secE"
                     /locus_tag="ACG8V3_000403"
     CDS             complement(25877..26047)
                     /gene="secE"
                     /locus_tag="ACG8V3_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0298974.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26071..26223)
                     /gene="rpmG"
                     /locus_tag="ACG8V3_000404"
     CDS             complement(26071..26223)
                     /gene="rpmG"
                     /locus_tag="ACG8V3_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0298975.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26385..27029
                     /gene="cbpA"
                     /locus_tag="ACG8V3_000405"
     CDS             26385..27029
                     /gene="cbpA"
                     /locus_tag="ACG8V3_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0298976.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27085..27588)
                     /locus_tag="ACG8V3_000406"
     CDS             complement(27085..27588)
                     /locus_tag="ACG8V3_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0298977.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27601..27647)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28185..29150
                     /locus_tag="ACG8V3_000407"
     CDS             28185..29150
                     /locus_tag="ACG8V3_000407"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0298978.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29249..30421)
                     /locus_tag="ACG8V3_000408"
     CDS             complement(29249..30421)
                     /locus_tag="ACG8V3_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0298979.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30408..31553)
                     /locus_tag="ACG8V3_000409"
     CDS             complement(30408..31553)
                     /locus_tag="ACG8V3_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0298980.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31567..32491)
                     /locus_tag="ACG8V3_000410"
                     /pseudo
     CDS             complement(31567..32491)
                     /locus_tag="ACG8V3_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32608..33687
                     /locus_tag="ACG8V3_000411"
     CDS             32608..33687
                     /locus_tag="ACG8V3_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0298981.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33684..34312
                     /locus_tag="ACG8V3_000412"
                     /pseudo
     CDS             33684..34312
                     /locus_tag="ACG8V3_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(34422..34649)
                     /locus_tag="ACG8V3_000413"
     CDS             complement(34422..34649)
                     /locus_tag="ACG8V3_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0298982.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34752..37340)
                     /locus_tag="ACG8V3_000414"
     CDS             complement(34752..37340)
                     /locus_tag="ACG8V3_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0298983.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37360..38019)
                     /locus_tag="ACG8V3_000415"
     CDS             complement(37360..38019)
                     /locus_tag="ACG8V3_000415"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0298984.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38068..38571)
                     /gene="trmL"
                     /locus_tag="ACG8V3_000416"
     CDS             complement(38068..38571)
                     /gene="trmL"
                     /locus_tag="ACG8V3_000416"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0298985.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38792..39658)
                     /locus_tag="ACG8V3_000417"
     CDS             complement(38792..39658)
                     /locus_tag="ACG8V3_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0298986.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39767..40408
                     /locus_tag="ACG8V3_000418"
     CDS             39767..40408
                     /locus_tag="ACG8V3_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0298987.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40470..41831)
                     /gene="mgtE"
                     /locus_tag="ACG8V3_000419"
     CDS             complement(40470..41831)
                     /gene="mgtE"
                     /locus_tag="ACG8V3_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0298988.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41849..42748)
                     /locus_tag="ACG8V3_000420"
     CDS             complement(41849..42748)
                     /locus_tag="ACG8V3_000420"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0298989.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42750..43547)
                     /locus_tag="ACG8V3_000421"
     CDS             complement(42750..43547)
                     /locus_tag="ACG8V3_000421"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0298990.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43525..44229)
                     /locus_tag="ACG8V3_000422"
     CDS             complement(43525..44229)
                     /locus_tag="ACG8V3_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0298991.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44360..44936
                     /locus_tag="ACG8V3_000423"
                     /pseudo
     CDS             44360..44936
                     /locus_tag="ACG8V3_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            45054..45731
                     /locus_tag="ACG8V3_000424"
     CDS             45054..45731
                     /locus_tag="ACG8V3_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0298992.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45810..47618)
                     /gene="pepF"
                     /locus_tag="ACG8V3_000425"
     CDS             complement(45810..47618)
                     /gene="pepF"
                     /locus_tag="ACG8V3_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0298993.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47794..48792)
                     /locus_tag="ACG8V3_000426"
     CDS             complement(47794..48792)
                     /locus_tag="ACG8V3_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0298994.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49018..49671)
                     /locus_tag="ACG8V3_000427"
     CDS             complement(49018..49671)
                     /locus_tag="ACG8V3_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0298995.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14963 a        10487 c         8770 g        15757 t
ORIGIN      
        1 ataattcacc aatctattta ttaaaggacc gggatctaat tcatattttt gtttttctaa
       61 ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat ctttacttat
      121 agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca ttttagcact
      181 tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata caagtagttg
      241 ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt ccataatatt
      301 ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac aaaattttta
      361 aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt ctaacattca
      421 ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg aataagaatt
      481 taatcacttt taagtatata aactatgtat attctttaat attttttgag aatgcacttc
      541 gtacataata aactttaatc acattagtct ttcataccta cgatcgatct ctttgactcg
      601 cacccataaa ttcataatag tttgtcggcg tctttggacg tttcgaagat gagctacgca
      661 ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa atttggatat
      721 ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt ttccagcttt
      781 cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga tcttttccat
      841 tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa ttgtctaata
      901 ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta tggatctgct
      961 cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat ttggcaaaca
     1021 tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc gcttgaccta
     1081 gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc aagttgtcat
     1141 atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg attcctatga
     1201 ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg taggctggca
     1261 ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact tgatctttat
     1321 attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg gcatctgact
     1381 cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc gttacatcat
     1441 agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct gtatcccatt
     1501 caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc cctataccaa
     1561 tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt tttttacttt
     1621 tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc tgtgtaaaac
     1681 ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca ttcccgactt
     1741 gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg atcatagagg
     1801 caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc atgatcgtac
     1861 ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc ccgaatgaat
     1921 cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta aaacgaacgg
     1981 ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg ataccgaaaa
     2041 atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag tcaaggatcg
     2101 gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat ggtaccccaa
     2161 tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc aaatcattcc
     2221 atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta agtcctggac
     2281 gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa ggagctggaa
     2341 tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt tgtaaaaatg
     2401 aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc cagtttgcca
     2461 ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt ttctgctaat
     2521 gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc tacaactgca
     2581 agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat gtctttccca
     2641 atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt cctagcttct
     2701 aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac agccatcagc
     2761 atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc agattcgata
     2821 ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga acgatcgact
     2881 tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc ttcacctgtt
     2941 ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat ccgtaaagct
     3001 tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg caggtccagt
     3061 aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc ttcgtccatc
     3121 agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg ttgcatccca
     3181 ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt ttctaatgct
     3241 ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa cccgtattct
     3301 gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac catattgatt
     3361 ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc ttctccatca
     3421 atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat caatgtggat
     3481 ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac gtcaaaattc
     3541 acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt cggctggtga
     3601 tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt taaatgtttt
     3661 acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt ctttctgaaa
     3721 aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa agaaatttgc
     3781 ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta gccaagttaa
     3841 tattttttta atatttcaca caatcattct gcaagctgat gctccgggta aaaaaagttt
     3901 gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc tgccgtaaat
     3961 ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc gataactgtg
     4021 gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg cattgctttt
     4081 tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg cttcgtttga
     4141 ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg cttctccaca
     4201 cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata ttgcaatagg
     4261 aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg tacttccatg
     4321 attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga agcattgtac
     4381 gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg gttttcaacg
     4441 atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat ttttttcttt
     4501 ccagacattt tctttcctct ttcttttttt actttccctc atcttactat gctctagcaa
     4561 aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt gatctgttcc
     4621 ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt taaacgagta
     4681 tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt cgccatccta
     4741 ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg gctgacggaa
     4801 caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta acgctacctg
     4861 tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat aagctaaacc
     4921 gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc gatggatagc
     4981 cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg aataagctgc
     5041 tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt cggatacttc
     5101 cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga ccaatcctgt
     5161 aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa caactgccag
     5221 gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag cataaaaatt
     5281 ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc ctgctttttt
     5341 aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt gaggaaaatg
     5401 atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac gtgttttttt
     5461 caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag aaggagctac
     5521 ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca actttgcaga
     5581 acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt tttcaatcgt
     5641 gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag gaatgaagga
     5701 ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga tcctcgccat
     5761 atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt gcggtagaaa
     5821 tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct gcatccatcc
     5881 cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg taccaaacaa
     5941 tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca gataatagat
     6001 cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg caatctgatc
     6061 gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga gtgtacatag
     6121 ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt cgtccaatag
     6181 tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg ctatctcgta
     6241 atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc gtttcttgcc
     6301 gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac gtggatcata
     6361 tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct tttcgattag
     6421 tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac caagaccata
     6481 ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca tcatattcag
     6541 accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa gatttcgttc
     6601 taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa ccaaataatc
     6661 tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg gtaggtcctg
     6721 aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg ttacgcccgg
     6781 ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag ttttcccgct
     6841 gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat caagattagt
     6901 tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc gattggatct
     6961 cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc catatccatt
     7021 aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt ctctcctgct
     7081 tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt gacaaagatt
     7141 tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa aatggcattg
     7201 ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat attttgcaaa
     7261 tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa attctgcttt
     7321 gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg tattaaaatc
     7381 attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat cattctcaca
     7441 tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga gcgaatcttt
     7501 agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac aattttttct
     7561 ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt caaaaacaca
     7621 aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt tgactttttt
     7681 actaagagtt ttatactatc tattatagat aataagaatg tgttagagga ggcactcacc
     7741 atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt caaacggttt
     7801 ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc tgaagcagta
     7861 gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga tgcagaaaca
     7921 gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga ctaaaagaat
     7981 caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt cgcaaacttc
     8041 caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca tcatcaaata
     8101 cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc agttatttca
     8161 agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat aaggataaag
     8221 aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact tttctatcta
     8281 ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa ttcattcaat
     8341 tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa catttttctt
     8401 tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa accaacgacg
     8461 gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt tactttgcga
     8521 acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa cgatatcagc
     8581 cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg gtgtcttgtt
     8641 tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac tctgctttgc
     8701 aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt gaagccggcg
     8761 ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg aaattattcg
     8821 aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc gtggtttatt
     8881 tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa aatgcagatg
     8941 aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa ttttatgaat
     9001 tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa aaagaatatg
     9061 gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt gcaaatccaa
     9121 acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca aaattacaac
     9181 aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag aatccaatcg
     9241 atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa acgccttctg
     9301 tgattaacgg caaacaggaa tatgaagtat taggaacgga tatctcatgt aacttaggct
     9361 cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg cgtacgatga
     9421 ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca ccgatccaaa
     9481 acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac acttatcttg
     9541 caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat atttacttca
     9601 tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa agaaaacaag
     9661 ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa aaatacgtaa
     9721 ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc atttttatcc
     9781 caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga ctgtatcatc
     9841 aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat acgagtgcaa
     9901 gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc ggtaaaattt
     9961 attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct gcttatgata
    10021 tggacatgcg taaagtaatc gatgtttatg caatggcaca acaacacgtc gatcaaggca
    10081 tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa tggaagacaa
    10141 caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc cacaaaggaa
    10201 tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc ggaagcaatc
    10261 aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata ttttaattaa
    10321 aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga aattgaagat
    10381 atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga tacacgtatc
    10441 ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa agatacagta
    10501 ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag tggcatggat
    10561 cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa tattcaattc
    10621 atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa tacgaaaaaa
    10681 gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa aaaagctgag
    10741 cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc cagcgtcttt
    10801 ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt aggaaacaac
    10861 aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc tgtacatgga
    10921 acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc agaacaagaa
    10981 acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga agaacgttat
    11041 acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt cctacgctat
    11101 aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga tactgccaat
    11161 gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca tgacttcttc
    11221 tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga cgacgattat
    11281 ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa ataaaaagag
    11341 tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat tcacttgttt
    11401 cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt ctcaataaat
    11461 agattttccg gatatttaat attatcgtct taatcactgc atagacaggt atgcaaatca
    11521 gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg atcgtcaaag
    11581 gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca tctaattgtt
    11641 ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata aacaaaccga
    11701 caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata ttcgtgattc
    11761 cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt gccaaaaagc
    11821 tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca gataatgttt
    11881 cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga aagaagtgag
    11941 acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata aatggaacgg
    12001 taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca cttgcagata
    12061 aactgactaa tatgatattg aacaaattac taagagataa atttgctgat gctaattgtt
    12121 cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac catttgcttg
    12181 tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc attaactgag
    12241 gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac gtcaataaaa
    12301 aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga tcgaacatat
    12361 aatagataaa accagcaaca agcaatggta aaaaaactgc cgaaagcatt ttcaatattg
    12421 gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg agtactagct
    12481 ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat ctcctccaag
    12541 gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga ttttcatttg
    12601 cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact taaacaaaaa
    12661 cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat aaacggtgtt
    12721 caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga actattttta
    12781 tttggctggg cttattactt gatgtaggac atctatttta caacctcaga tcatttattg
    12841 cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact cctcgatcac
    12901 atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt acactcggta
    12961 gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt tctcttcaaa
    13021 ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt ctaacttttt
    13081 agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa tcccgataaa
    13141 actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc cagcaattgc
    13201 ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga tgatcgttcc
    13261 cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt catctgaacg
    13321 aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac tagcgatctc
    13381 aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg tacgcatacc
    13441 tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata gcatagcgat
    13501 ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct aaacaccctc
    13561 cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt tacctcttac
    13621 gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa cggtgttcaa
    13681 tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat ttttcctttg
    13741 tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct actattgaaa
    13801 attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg tgattttgca
    13861 atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg attgcatcag
    13921 ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt gaaccgattc
    13981 ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt ttgtttgcat
    14041 tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa gcaccaggtt
    14101 cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg attggatgtg
    14161 catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct tttccatctg
    14221 gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg aaatgacggt
    14281 agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca gaagtcattt
    14341 cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt gtgcttcctg
    14401 ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt tcaaatgctt
    14461 tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt gctcctttat
    14521 attttggtat agcccaatta tagcattctt tttttctttt tacaataatt agtatagacc
    14581 aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg tttcataaga
    14641 tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac tgtggacgct
    14701 ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac attaaacaac
    14761 attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca attaaatgct
    14821 ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt gattaaaatc
    14881 atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa tattcacaaa
    14941 ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga atttctaaaa
    15001 actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta ccaaacatat
    15061 agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac ggaattagac
    15121 caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg gttggggatc
    15181 gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg ttcaaaggtg
    15241 attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga accaaccaat
    15301 cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt tgacggtgcg
    15361 tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca tatcgcagac
    15421 atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt gaaacaaaag
    15481 gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca aatcgaaaag
    15541 accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc aaaaagccga
    15601 caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag taaagctgtc
    15661 tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac tgatttagtc
    15721 atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt tggtgaaaat
    15781 attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac actgatcggt
    15841 gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat caattacttt
    15901 tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt aagcagtcaa
    15961 tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg gttagttaat
    16021 caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa ggtgaaactt
    16081 gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac gaaccatatc
    16141 gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg aacagtaatc
    16201 atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat tgagattgaa
    16261 ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa gttttgtcac
    16321 aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta gctcaaaaaa
    16381 cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg cagaacagct
    16441 tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct ttaaaaacaa
    16501 ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt acacctaacc
    16561 agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct ccaataaagt
    16621 acataaacat aaatacagta ttatttcggt tgcttgctgc ttcacctagc ccctgtactc
    16681 gtgtttgatt ggcgacttga ccaaattgat tcccgacgtc taataaaata atagctaaaa
    16741 gcaaaatggc caaatggctt ccgccaacca ataaagcaat aaagcttatt gtttgcataa
    16801 caagtcccat atacacgatt tttctttcag aataagaatc tgccagtcgt cccacgatag
    16861 gtgcagcaac tgctccagac aaaccaaaaa tcgctaatat ccctgcttcg aatgttcccc
    16921 aattgtaggc gggactgctg atataaaaaa tcaacgtaga ccaaaagatc gaaaatgttc
    16981 caaacatgaa aaatccactg actgccgctt ctcttaatag acgttgagaa gcaactaaat
    17041 gcggcaaact tttaagtgaa tcaaggtatg aaacagctgt actactttgc tggacagaac
    17101 gcggcatttt ccagtaaagc agaagtgtaa gaattccgac tgcacataaa gcaaataaat
    17161 agatcatccg ccaagaaaaa agactggcaa caataccaga taccgtccga gaaagcaaaa
    17221 tccccacgag taaaccgcta agcaatgtac ccatgacctt ccctcttgct tttggtcccg
    17281 ctagtaccgc accgtaagga atgatgattt gcggcacgat cgaaagtaat cctattaaaa
    17341 aagaagcgca tgcaaataac gtaaacgaag gcgcaaaaaa agcggccaac agagagacgg
    17401 cagataacgc tgccattcga atgatcaatt ttggacgatc caccacgtcc cctaaaggaa
    17461 ctaaaaacag taaccctaac gcatagccta attgggtcaa catcgtcaat atcccgactg
    17521 cacttgtgct aacagataaa ctagctgcta cttttgttcc aatcggctgg atgtaataca
    17581 tattggcaac aacaacgcca caagtgattg ataatagtaa aataagcgaa gttgttaaag
    17641 gttttttttc attactcttc attgatttcc cccatttcct atcttagttt acaacttatg
    17701 gaaaagattg gaaatagaaa aatacaacta ttcgccacac ttattatttg tgcttttgtt
    17761 ttccttgttt tttcttgcga tctttataaa tgaagacaac taaccctact agcacagtaa
    17821 cacttgcaac caacgtgact gcagttttgt tatatccgtg aaacagagct tctccaccac
    17881 acgcatacaa cacagacgaa gggatgactc caatcacgat cgacaatatg atttgctgta
    17941 gaggcaattt catcgcatct gcagcaaaat tgaccataga agatggaata accggtatca
    18001 tatagcctaa cattacacct atttttggat gtttcatttg tcggattgct tgcacccaat
    18061 gattcgtttc ggttttctta tctaaaaatt ttaagcgatg cataaggaaa attgcagtga
    18121 tgttaccaag agcattaccc aaaatgttga tgatcgcacc tgtcagtggt ccaaagctca
    18181 aaccagcaat gactccaact actgaagtag gaatacctgg aacagcacac atcaccgctg
    18241 tcaatacgat caagagtatc gatgcaaaag tcccatgcga acgaatatgt gcgagtagct
    18301 ctgccttact cgctctcgga tttaaaaata attggatgtc tgtctgatat tctaaataca
    18361 accgatagcc cactaccagt atcagaatga ttcccaacga gatcaatatc aatcggatta
    18421 ttttcttttt catatttcct cctgaaaaaa atctatattc attaaataat agtttaacat
    18481 aaaaacaaaa aagcaccgaa atccaacata ggatctcagt gcttttaaat agtaaaaatt
    18541 atttaacagt tacagatgcg ccaacttctt caagtttagc tttcaattct tcagcttctt
    18601 ctttagatac gccttcttta actggtgcag gagcgccatc aactacagct ttagcttctt
    18661 tcaagcctaa accagttgct tcacgaactg ctttgataac tttaactttt tggtctccag
    18721 ctgcagttaa ttctacagtg aattctgttt gttcttcagc agcagcagcg ccaccagcag
    18781 cagctacagc tacaggagca gcagcagata cgccaaattc ttcttcaata gctttaacta
    18841 ggtcgttaag ttctaggata gttgcttctt ttaactcagc aacgatgttt tcaatgttca
    18901 atgccattat aatttcctcc gtttaattgt tcttttttat ttaatgcttt atgcggcaat
    18961 taagctgcat ctccatcttc tttgtctgcc actgctttga cagcgtaagc cacgttgcgg
    19021 actggcgctt gtagtacaga tagcaacata gatagaagtc cttcgcgatt tggcaatttt
    19081 gccaatgctg tgatttcttc caatgaagag actttacctt cgatgatacc gcctttgatt
    19141 tctaatgctt ttgcttcttt agcaaattcg tccatgattt tcgctggtgc gactacatct
    19201 tcgttactga atgcaacagc tgtaggtcca gtgaatactt cgtccatgcc ttcaaggcca
    19261 gcttttttag ctgcacgtga aagaatagaa tttttgataa ctttcatttc tacgttagca
    19321 tcacgaagtt gtttacgtaa acgtgttact tcatcaactg ttaatccgcg gtagtcaacg
    19381 actactactg atgcagctgc tgtaaatttt tcgcttactt catcaacgat tgctgctttt
    19441 tttgcgataa ttgcttcact catttttgag tttcacctcc tgaattttga tggaagagtt
    19501 ttttgcactg aaaaggcaag tcacttttat agttactttg ctcaaccagt attattcatc
    19561 acctattcac ttcgttcata gtgattctac tcgctttgct cgaaaggaaa ctcctttcgc
    19621 tttgctcaac cagtattgtt catcatctct tcacttcgtt catagtgatt cacaataaaa
    19681 aactctatgc caccgcagac atagagggac tattgaatat attcaataaa cgtcctcggt
    19741 aggaaattaa ggcttgatgc cacctactgt cttcggtaca agttaattat taacctcaac
    19801 tagcttagca tgctaaacta gttgagtcaa gaattatttt tcagttggat caatcaacat
    19861 tctaatattt aattagaaag aagcttggtc aacatggata ccaggtccaa atgttgttgt
    19921 gatcgttaca tttttcatgt attgaccttt agctgctgat ggtttagctt ttagcaatac
    19981 atcatggatt gctgcaaagt tttcaactaa tttttcatcg tcaaatgaaa cttttccgat
    20041 tggtacatgg atatttccag ctttgtccac acggtaagtt actttaccag ctttcacttc
    20101 gtttactgct tttgttacgt ccattgtaac tgtaccagtt ttagggtttg gcattaagcc
    20161 ttttggtcct aatactcgtc ctagacgacc tacagtagcc atcatatcag gtgttgcaac
    20221 aactacatca aagtcaaacc atccgttttg gattttttga accatgtcgt cgtcacctac
    20281 gaaatctgca ccagctgctt cagcttcttt tgctttgtct cctttagcaa aaactaaaac
    20341 agtttgtgtt ttaccagttc cgtttggtaa tacaacagca ccacggattt gttggtctgc
    20401 ttttttaggg tctacgttca aacggtatgc aacttcaact gttgcatcaa attttgcaat
    20461 gtttgtttct tttgctaaag ctactgcttc ggcaacagga taaactttac ttgaatcaac
    20521 tttttttaat gcatcttgca tttttttgct ctttttagcc attttggttc ctccttgatt
    20581 gtggttataa cggttgtacc tcccacgtgt tctatatctt tcaatataga gccaactgag
    20641 aagctgctgt cttagggtgt atagattatt ctacagtgat ccccatgctt cgtgcagttc
    20701 cttcaaccat gcgcattgct gcttcaacgt tagctgcgtt taggtcttcc atttttaatt
    20761 cagcgatttc tcttacttga tcgctagaaa ctttcgcaac tttatttttg tttggttcgc
    20821 ctgaaccttt ttcgatttta gctgcttttt tcaatagaac agcagctggc ggtgtttttg
    20881 taacgaatgt gaatgaacgg tcttcgtata cagaaataac aaccggaatg atcaaacctg
    20941 cttgatcagc tgtacgagcg ttgaattctt ttgtgaatcc catgatattg atacccgctt
    21001 gacctagtgc aggacctact ggtggagctg gagttgcttt acctgcagga atttgcaatt
    21061 taacgatttt ttctactttc tttgccacga gacatacctc cttaagtccg tgatgtggtt
    21121 aattggagat gcatatttct cctcccacgt tttatctgat ctgaaaaaat ccagacaaat
    21181 aatgtgcaaa acaatgcaca ctggtacagt atagcaaatt tatttgtttt ttcaagattt
    21241 ttttctgcta tttttacatt ttgcttgaat aaacgctaaa tttattctaa aaaaacaaca
    21301 atttttcgtt ctcatttctt cttttatgtt acacttgcgc tagcatggtt caacagatga
    21361 aagcaaacaa taacacggtt ttttagaccc ttgtacaaag attcctacat aacaaaatga
    21421 ggtggagaaa atgaaagatt taaggtatag aagtgtcttt gatattattg gacctgtgat
    21481 gattggtcca agtagctctc acactgccgg agctgctaga atcgggaaaa tcatacgtag
    21541 tatatttggc gaacagcctg attctgtaga tatttatcta tatgaatcat ttgcaaaaac
    21601 ctatcggggg cacggaacag atattgcatt agttggcggc ctgttaggga tggaacccga
    21661 tgatgaacgt ttagctgatt ctttgaaatt ggcatatgaa gccggtatgg aagtcttgtt
    21721 tgttcctaag agtgaaaaag ccgaacatcc aaactctgta aagatgcttc taaaaaaagg
    21781 aacacgaaag ctttcagtca ctggcatttc gatcggcggt ggcaatatcc agatttctga
    21841 attgaatgga ttcaaaattt ctttaagcat gggtactcct accttgatcg ttgtccacca
    21901 agatgttcca ggtatgattg ctaaagtgac tagtatactt tctgagacca atacgaatat
    21961 cggcactatg acagtgacac gggaatcaaa aggtgaaaat gccattatga taattgaagt
    22021 agatgatcct caagtagaag aaacggtaaa aagactcaaa cagctgccaa atatcgacag
    22081 cgtaaattat ttcgattaag gagctaaaaa aatgttttta tcgattgaag aattagtaga
    22141 tcaagcaaaa gcttatccaa gtgtagcaga attgatgatt gcagttgaaa tggaaatgag
    22201 tggccgttcc cgtagtcaca tcatcgagat catggaaaaa aacctctcag taatggaaca
    22261 atcaatcaac gagggaaaag aaggtgttac ctccgtcaca ggaattaccg gtggtgatgc
    22321 aaaaaaatta gatgattatt taaatgaggc agacttttta agtggagaaa caatcttaaa
    22381 agctgtacgc aatgctgtag ctgttaatga agtcaatgca aaaatggggt tgatttgtgc
    22441 tacaccgact gccggaagcg cgggtgttgt tcctggtgtg ctgatggctg tccgagatcg
    22501 gcttgatttg acacatgaac agcaattgga attcttattt accgctggcg ctttcgggtt
    22561 agtcatcgca aacaacgctt ctatcagtgg tgcagaaggg ggatgtcaag cagaagttgg
    22621 ttctgcaagt gcaatggcag cagcagcttt ggtttgtgca aaaaatggga ctcctcatca
    22681 agcagcacaa gcagtagcca ttacattgaa aaatatgatg ggactgattt gcgatcccgt
    22741 tgcaggctta gttgaagtcc cgtgtgtcaa acgtaatgct ttaggctctt ctcaagcttt
    22801 tatctctgct gatatggcat tagcgggtat cgaaagtgtc attccgccgg atgaagtcgt
    22861 tgctgcaatg taccaagtcg gacgtcaaat gccgtcgatt ttcaaagaaa cagcagaagg
    22921 tggcttagct gttacgccaa ctgctaaacg acttgcggaa gagattttaa ataagagggc
    22981 gtgataaagc tccttaattt cgagtattaa gagattagtt atcttcataa caaaaaacga
    23041 agagaggact aattgccctt ctcttcgttt tttattaatc catcaatttc gtatatttta
    23101 cttcatcgat cgtttttgtt cctgcaagtt gcatcgtgat cgacaattct ttgttcaggt
    23161 gttcaaaaac agatgttact ccttctgccc cacctagatt caacccataa atcactggtc
    23221 ggccaatcgc aactaagtca gcaccacttg ccagagcttt gaagacatgc tctcctcgac
    23281 ggacaccaga gtcgaaaatc actggtacac gtttgttgac tacttcggca atttttggca
    23341 atacttcaaa ggacgcagga cctccatcta gttgacgtcc accatgattg gatacccaga
    23401 taccatctgc ccctgcagag atagcgatta ctgcatcttc tggcgattgt attcctttta
    23461 cgataactgg aagatgggtg atttctttga ttgttttgat gtcatctggt gtcaagcctt
    23521 gttttgcagc tgcataaatt tctgcgattc cttttccttc gccattacca cttgcacttt
    23581 gttcgctata cgcagctaag ttaggcattg gtaatggaaa ttggaattga ttgatgacgt
    23641 cttcttcacg gtaaccaccg agcgttgaat ctgccgttaa aatgatagct ttagcccctg
    23701 cttctactgc tttgttcaaa atgaactcat tgaatctgtc gtctttactc atatataatt
    23761 ggaaaaactg cggtgcgcct ggcgctgcat cggctgcgtc tttgattgtc gtatttgcat
    23821 aagtgctgat ggaaaagatt gatccagcag cagctactcc tttagctgta tcagcttccc
    23881 ctttttcatg tgccaatcct tgggccgctg atggcgcttg gataatcggt gtatctaatt
    23941 caattccgaa aacagatgta tgcaaatcgg ctgagtcgat tccgcgtaat atccgtggca
    24001 tgattttctt tgtattaaaa gaagtcgtat tttctttcat cgtccactca tcttcagaac
    24061 caccgcgaat atagccaaaa gctcctgctt ccatgcgctc ttttacacga ccttctagtt
    24121 aggcaatgtt tacaatatcg attggatgtt cttcattact tgtatgataa gtcatagtag
    24181 atccttcttt catcttcttg attacatata aatcatacta aacttacaaa aagtaagcaa
    24241 acatttactc tcagatactt ttatcgctat tctttccaaa taaatcgact tacaagctga
    24301 tctacttctt ggttttcatg aagcatacta tgagaggcat tttcacctgt cactactttt
    24361 tcctctattt catttccgcg ttgcttcaac aacgcataga ccgctaatgc actgtttaac
    24421 ggtactgttc catcactcaa gtctgtttca tctagttgac cagcaatcaa gaaaaatcga
    24481 gtggttatag gaatattacc gatcatttgc tggtaatctt gatagcggtt agaaacgaca
    24541 gcaggcccct ttgctaactc atctgtcaga gactgcgcac tatcatcgac aaaatcatta
    24601 aaaggcgcgc caattgctgt taatgtctga acttgtggca atgacgtatc ctgtccatac
    24661 gttcctaaat agcgtagtgc actaactcct cccatggaat gccccactat gttgacttta
    24721 tctatgccgt agttttcttt cagatacaaa agacaagttt tgatccattc tgcttgattc
    24781 cactcgttat ttttattatc ttcaaacaat acttggacca tcggattgtc tgcttttcca
    24841 gacaaatctc ctgttgcttg tatttgtcca tctgttccta ctgttaaaac caattctttt
    24901 actgcttgct gatttttttc caaacgccgc aacatccctt taaaggaatt ttctgtcccg
    24961 ctgtagccgt ggaaaaaaag tgttggaata gtggtttttt ctatcacttt actggattct
    25021 gccgtttttt ctttatcaga aacttcagta gtatttatct tcgtcgtctg gcaaccactt
    25081 aacgcaaaca aactgataag gactaaagct gctgcagttg cacgtttgca taaatttttg
    25141 attggttctg tcatacacct cttcctttcc atatagaaaa aagaaagact tgaaaggcca
    25201 atcatatgat cgacatttca agtctcttta ttaatcgatt ttatctactt gatcgaaatc
    25261 aagttctgta ctggtttcac gtccaaacat atcgatgttg actttcaatt tttctttttc
    25321 tccgtcaact tctgttactt gtccttctaa tccagcaaat gcgccttcga tgattttcac
    25381 gacttcgcca acttctactt ctagatcatt ttgtcgcagg ctcataccga tagaacgcaa
    25441 gatatgattg acttcttctg gaagtaatgg tgctggtttg cttccggctc cgtgtgaacc
    25501 aacgaatcct gtcacaccag gtgtgttgcg gacaacatac catgaagcgt ctgtcatgat
    25561 catttcaaca agtacgtatc ctgggaatgt tttatgaacg atttcttttt ctttcccatt
    25621 tttgacttca gtttcttttt cttcaggaac aacgacacgg aaaataaagt cggccatttt
    25681 catactttgc gcacgagatt cgatatttgc ttttactttg ttttcatatc ctgaatatgt
    25741 atgcaagaca taccagtttt tttcaaaaga ttccattgaa ggctcctcat ttcattgtaa
    25801 aataaaaaaa cctcagtaca cccgaagttt ttctttcgct tcattatagc actaaaagta
    25861 cgttgtggct agcgagttat tttaaaatcc agccaaataa gctttggatg gctgtatcca
    25921 ttacgtaaaa catcaatccg aaaataatag aagtttcgat gactactaac gtatctttac
    25981 ggagttgttt tctgcttggc caagttactt gtttgatttc atcttttacg ctttttagaa
    26041 atttcatgag acaacctccc ttgctactgt ttatttcgtt tctttatgta atgtatattg
    26101 attacaatat ttacaaaact tattgatttc caaacgttcg ccgctttttc cttcactgac
    26161 tgattttgtg tagttgcgag acccacaaac ggaacaagcg agtgctgctt ttttctttgc
    26221 catttacttc tgcccccttg ctgagaatca gacttatact tcataacagt atcacgtaac
    26281 tttttacttg tcaatttgtg aaataaacta tctattgttt tggataaaat tatgatatga
    26341 ttaatttgaa attaagctaa aatgagacaa ttggaggtat agcgatgcta ttaaaaacac
    26401 ttgtatataa gaaaaaagat ctgacaaccg ttacagagac tagtacatta gaagaagcat
    26461 taaaaatttt agaaacttcc ggataccgtt gtgtgccgat cttagattct tcaggtaata
    26521 ttttccgtgg aaacatttac aaaatgcata tctatcgtca taaagcaaat ggcggtgata
    26581 tgacgcttcc tgtcactcat cttttgaaaa acgctacaaa gttcatttac ctggatactt
    26641 ctttcttcaa agtattcttt acgatcaaag agcttcctta tattgctgtc cttgacagtg
    26701 aaaatcattt ttatggtatt ttgacacata gtacgttgct taatatgctc tctcaatctt
    26761 ggagcgtaga acaaggaagc tatgtactga caattgcttc cacaggaaag caaggagatt
    26821 tggctgctat tgcaaaaatc atttcaaaac acagcagcat tgccagctgt attactcttg
    26881 atatcgaaaa agacgaatac attcgacgta cattgatcac gctaccggct aacactgaag
    26941 cgaaagtatg tgaaacgatc gtcaaccact tagagaaaaa gaattataaa gtggtagaaa
    27001 ttgaaaattt gcaaaaccaa gaagcataat caaaaagcga aaaaagaaaa cagctaatct
    27061 gctttctttt ttcgcttttt ataattagac acgcttttca aaatcaagcg cgcgatctaa
    27121 ataatacatc aatggtgctg aaacagccat gacgataaac tcacttaatg caagcgtgcc
    27181 ataggttgtc caaaatgctt ggccacctgt tggaactaac atataagcaa tcagtcccat
    27241 gctgatggta aagaagatcg tgttcaatac taaacgcatc tttacatttt tcactttgtt
    27301 ttgcaataaa gctgtcaggc ctaaagaaag caatgtttgg ccgccaccaa aaaccacatc
    27361 catgatacca aaaccaaaga aggcattata gacgatgacc ccgcctaaaa ctccccaaag
    27421 caatttacgg ttaaagacaa ctaaatgatt caaactttct gaaatacgaa actggatcgg
    27481 ccctgaggat acaggcgcaa ctaaaatagt taacgctaga taaagagcca tgatgatgcc
    27541 atttacaacg attacggata accgttgtct gcttttcgtt ttttgcattt tctctcctct
    27601 tttctcctag tttttttaac gcgggatggt tgatgaaccg cggttatgtg aaacaaacaa
    27661 taattataac agacttttca tttatttcaa tccgtcttat ctgcttttaa aaaagcaaaa
    27721 gcttaaacaa tgtaaacgtt tgctaaaaat cgtgggatat ggtataaagt aagtgtaagg
    27781 ggtcataaga attcccagta aagcagacga agtaggatct gcaagaacag aattgttgtg
    27841 aaacttaaat aaataaaaaa ggatgtggga atttgagcaa cctcttacta gagaacggtc
    27901 tagcaagcag ctgaagatct aagtggacat gccgaacctt ggaacttctc cttacgtaag
    27961 taatggatca tgcgcttgct aggctttttt gtgtttttcg gtcttcggac ctttttcatt
    28021 ttcctagact tctgctataa taaccaagaa ttaattttgt aacagatttt tttgaaaaaa
    28081 caagtttctt tcattcaaaa aatagaaaaa gcttgctaat tattggttaa ccagcaaaca
    28141 ggagtattaa aatatcttgt tttattgaat agcaggagga aatcatgaaa ttccattatc
    28201 atatattgat caatccatcg gcaggcagcg gaaatggcta taaagtagca gaaagaattt
    28261 taccggtatt aaaaaataag catattgact atacacttca ttatagtgaa tacaaaggac
    28321 acgatgctga gattgctgaa actttggcaa aagaaacatt gattccttgg atagaagaag
    28381 aacagaaaac aatcgatact ttcccccttt tgatcatcgt aggaggtgac ggcaccttac
    28441 atcaggtact tgatactttc tatcagatgg aagtagaatt cccagttgct tatattccag
    28501 caggttctgg agatgatttt gctcgagggg caggccttcc aaaaaatccc aaaaaaggac
    28561 ttcagctgat tttagcagct caatcaccag aaaaagtcca cataatggct tatgaagaaa
    28621 aaataagtga aaaaaaagga atagctgtta ataatttcgg tataggatta gatgcagcta
    28681 tcgtccatgc gacaaatcat tcttccacaa aaaagcgatt gaacaaatac aacttaggat
    28741 cattttcgta tttattctct attctccgtg ctttattcac tcaaaaagga tttccaattc
    28801 tggtagatgc aggaggaaaa caattaagtt tttcaaacgc atttttatgt acagcaacga
    28861 agcatccttt ttttggcgga ggaatcgcga ttgttccaac tgctgatgcg tctaaaccag
    28921 tcattgattt tgtcgtggtc gaacgaatca atttctttaa aatcttgtgg ctcattcttt
    28981 tacttataca gaaaaaacaa ttgaaatcga aatatttcca tcactacaca acaaacagat
    29041 tacgtatcgt ttcaacaaca ccgcaacatg gtcaagaaga tggagaagaa atggaaaaac
    29101 gaccttttga tatcacaata accacaacta aacagttgtt ttggtgttaa taaaagaggt
    29161 tttgtcagaa gctacatctg gaacaccgtt tattcggaaa aaaggactgt gacacgactt
    29221 ttgtcacagt ccttttttta ctatgcagtt aattctttgc ccaattgggt tgccatcgat
    29281 agtttctttc tttacccaaa ataaattgga tcattgaaaa caccgcagca aaagcaacta
    29341 agacacaacc attgaaccaa aaagattctg caaatgtttg attgtaactg agtgtttcac
    29401 tgatcaagtt attattttga acaaaccaat atgttggtgt gaatgccgct attttgctaa
    29461 ccatttctgg caagtattct gctggtacaa acaccccacc gataaagcaa ctgcctgtga
    29521 taaaaatatc tttgatccca ccgctggttt ctgagttttt taccaatgaa gtgatacaag
    29581 ccgaaaagct aatgatcggc aataaaaaca agaaagtatt taaaatatga taccataccg
    29641 ttatatccca cgaggtgtgg atcactaaca gaaccattag aagtaagaag ccactgaaca
    29701 acagcagcga gtaactaatc aaactaaagg ttactttgcg attcaagttt cgtttggtaa
    29761 tgggcgcact ctggtttcgt ttactgatct ctttgcgatt aaatgctaga ttgatgacgc
    29821 tgaaaccgct aaaaatggat agaaacaacc cataagacaa gaaattaaag gttgtgcccg
    29881 tcaacagctt tttcatccgc tgactatatc ccgcacttag ctcaacttct ccagtaaccg
    29941 agagtgccat ttgtgtctgt tccatagctg catcgattgt tttatcttct tcttgaaaca
    30001 gaatcagtgt ttgcaaaaag gtgttcactt gctgtgtaac aactgtttta gaaaaggcat
    30061 ctgggcgact ctgtatcttg atcgtcggca tctttcctac ttcgacttgt tcggtaaagt
    30121 ctgcaggcag atgtaagata atgtttacac gattaaaata aagagcatca tcgatttctt
    30181 tttgtgaggt tccatccaat gtaacgactg tttccttgtc attcaaatat ttacgaaggc
    30241 catcgactac aggcgactct tcttcactaa tcaaggcgat tttcgcatct tgcagagagg
    30301 tattttcaac atcaatattt tgcgcaaaaa agaatgtggc aacaacagtg ataattacac
    30361 ctatcagtaa attcgattta tttgctttaa gcaatgtcca aatcgtttta taaagcgtca
    30421 taacgcaccc ctctttccat ccaagcattt cctaacgcaa aaaggaaaat aaatccgatc
    30481 aaccatagaa gattggtata aaaactagcc aaatcagtgt agaaataaag tttatacaaa
    30541 ctttcgctga tcaagttgac gagatttaat tgaccaacaa tcggaatatg aagatcaatc
    30601 caatacttca cttgttccga ccccatcata ccagcaaaaa agctcatcgc aatcgtcact
    30661 gaaatcaaga tgctatcctt ttgcaccaga ttcatttttg gtatcaaatt accaaccaat
    30721 gtacctaaca aaatcgcaca caacgacccc agagcggtaa caatcatcaa ccattgccaa
    30781 cgttcaccaa agtctacttg atagataaaa cgaaagacag ctaacataat ataaatctct
    30841 gtcaaaacaa tcgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta
    30901 ggaatcaagg acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg
    30961 cggacccccc acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa
    31021 cttttgaaac tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa
    31081 acagtcatga tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata
    31141 ccaatacgct gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct
    31201 tgacccacat ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct
    31261 gcatttagct tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta
    31321 tcattgactt cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc
    31381 gctttgatta caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca
    31441 aaggccgcca taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg
    31501 gatttatttc gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc
    31561 cctcctctag tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc
    31621 ttctttggtt gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg
    31681 caacaatact gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat
    31741 tccatcagaa gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg
    31801 tcaaacgatc cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca
    31861 cctgtccttg atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat
    31921 gtgttgtata gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt
    31981 tattccgact ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct
    32041 tgtgggcaat cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg
    32101 gacgaaacga aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt
    32161 tccttttctc tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac
    32221 ttagttcgtc aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat
    32281 aattttcagg agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca
    32341 aagaaagcat acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta
    32401 agacttctcc ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc
    32461 gtttgactaa tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt
    32521 gatctgacta taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg
    32581 acatttgtca caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc
    32641 tttctactgg ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt
    32701 atctatttat tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca
    32761 aattggattt tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg
    32821 tttctgccag cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt
    32881 ttgttaggtt ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt
    32941 gtcgtaattt tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat
    33001 cttgagaaaa agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg
    33061 gaacaaaata aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa
    33121 gaacggaacc gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg
    33181 attttacaaa ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta
    33241 gaaaatttaa gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac
    33301 ctccatgaga catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct
    33361 tcttctgtta cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc
    33421 ttcttaatgg tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc
    33481 gttgttttac attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc
    33541 accagcccac aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt
    33601 caagaacaaa agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc
    33661 attttaccaa gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt
    33721 gacgaaatcg ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg
    33781 tcattgtgcc aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac
    33841 ggacatccgc atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt
    33901 tcccaaagcc atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc
    33961 attttccatc ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc
    34021 tgtcaaatcc gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac
    34081 tcaaatgatg aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg
    34141 tgagcttgca atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc
    34201 cttgtacttg agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga
    34261 tttacgggat cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc
    34321 taacacaaaa aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct
    34381 attcaattgt cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg
    34441 ccaggattcg ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt
    34501 ttttctagtt ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg
    34561 aattcttttt ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc
    34621 gctaatttat cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta
    34681 gcatatcaca ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct
    34741 catctttaaa atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata
    34801 ccaatcatcg gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta
    34861 gaaacagcag gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct
    34921 ggttccactg tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca
    34981 ggtagctttt cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca
    35041 tttccgacca aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta
    35101 tcattatcca tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca
    35161 caagtgacat aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact
    35221 gccgtgtaga gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata
    35281 accatcttga attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt
    35341 ttattccatt cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca
    35401 tccactttat cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca
    35461 ttgaatacat tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa
    35521 acggtgtcgt tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc
    35581 atcattttat ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg
    35641 atatcttgtg cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt
    35701 tcaatttgat aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct
    35761 tttggcagac gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct
    35821 tgccgataaa tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg
    35881 agtacttgac caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc
    35941 atattcgtcg gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc
    36001 tcatcaacaa tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc
    36061 cgtccattca gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc
    36121 attcgtttag ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta
    36181 tattttgaag gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg
    36241 atcacagtag ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt
    36301 gcttcagcga tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata
    36361 cgccgaatat tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga
    36421 aggcgctgga tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga
    36481 ttagtctctt cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc
    36541 tgatcaggct ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc
    36601 aatgtctccg ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc
    36661 aagatagctg ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga
    36721 ttatctgcac gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct
    36781 tcgatgatat ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta
    36841 ccgaacccgt aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc
    36901 gtctcagcaa gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa
    36961 ggatcagcaa tgattttttc gatagcatct tctcccagca attctacaat tttttctgct
    37021 gttttcttcc cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca
    37081 gttgttggct tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga
    37141 tccactaatt ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg
    37201 gtgactacaa tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc
    37261 actttgtaaa aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa
    37321 ggcatttctt ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc
    37381 tgtttcagtt cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct
    37441 gcaaggctct tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg
    37501 caactcactt agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg
    37561 tcctacgaat aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc
    37621 gatacgtgtg attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc
    37681 taaatgatta cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga
    37741 ctgaccttct aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac
    37801 cggacgagca agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact
    37861 ggaataaatt gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc
    37921 gtaactttgt ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt
    37981 ccattgtgtc ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt
    38041 cttttatttt actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt
    38101 caattctaaa ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt
    38161 attcgataaa ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc
    38221 ttcgttttca cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa
    38281 gtgctcttct ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat
    38341 caaatgtagt ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt
    38401 aatatttaca tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga
    38461 aaaacctagt ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat
    38521 atttccagtg tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat
    38581 tcctctcata tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa
    38641 cgtatcaact cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac
    38701 ttttatcaga acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac
    38761 accctcaact tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct
    38821 tagttccttt taataattct atgtcaattg taatcttctt caaaggattc ttatataagt
    38881 ctgctttgat tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt
    38941 gattttcctc tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg
    39001 cttcagcaaa cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg
    39061 gataaaaagc ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt
    39121 ctccatcttc tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta
    39181 aaaccgcgtt gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa
    39241 atgcttcggc agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa
    39301 cagcattttg aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc
    39361 caacatctaa tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct
    39421 gatacatgcc agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg
    39481 ttttaaccgc atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat
    39541 tatttttgca tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc
    39601 ggaacagctg atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag
    39661 ttccccctca tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat
    39721 atgatacaat gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt
    39781 ttgcgctgaa aactacacat ctattcctct agcaattgct agaggagccg atcggatcga
    39841 attgtgtgac aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac
    39901 gttaagttat gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg
    39961 cgacttcgtc tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa
    40021 gaaattaggg acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga
    40081 agaggcctta gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc
    40141 ttttgacagt attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg
    40201 tgttgaccgt atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc
    40261 acggcttaaa gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg
    40321 tgtcactgca gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg
    40381 aaccaaaatc gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca
    40441 taatgcttat tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca
    40501 atattgaaat aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt
    40561 gctactgctg gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga
    40621 ttcgccactg tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta
    40681 tgtttccaaa aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg
    40741 ccggtcaaga cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc
    40801 gcaagtcgac gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta
    40861 atcagcgaaa tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg
    40921 attaaagaag cggtcgacat acctaaaaac aacagagtga tcagccaagg caaacgtcga
    40981 gacgctgcct ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa
    41041 taatcgctag cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct
    41101 aacaaatgat catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt
    41161 tgtgccacat cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga
    41221 tcagaaatca ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact
    41281 agctgacctt cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg
    41341 ttcttcagca cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca
    41401 gttgtcatga tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg
    41461 gcatcgtctg ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta
    41521 agcaagtcaa cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga
    41581 cgcatttcat ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata
    41641 tccgctaatt ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga
    41701 gattggtaga attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc
    41761 tgaatattat tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga
    41821 ttttcatcca aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca
    41881 tgtctgcggc taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac
    41941 gcagatcata acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca
    42001 tatcatcacc gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc
    42061 ttcctgtatg cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt
    42121 actcagtctg tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat
    42181 ctcgggcgat cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga
    42241 ctaaggcctg atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt
    42301 gcgcaaatcc atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca
    42361 ccacatggat tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag
    42421 ccattgtccc atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta
    42481 ctaccagaac gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg
    42541 tttcatgttc tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa
    42601 acaagacatt ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg
    42661 ctaacagacc ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat
    42721 ctttctcata atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca
    42781 cgatgccaaa aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga
    42841 taataaattg agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa
    42901 tcttctgtat tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa
    42961 cccaaagtac gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg
    43021 atactaggat ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc
    43081 gtagaaatcg tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca
    43141 tcggctacca tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc
    43201 tggtccggtg tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt
    43261 tgttcccggt tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc
    43321 gtgtaaaacc ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga
    43381 ctgtagtgat ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct
    43441 ggctcatgat catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc
    43501 acttctatag tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc
    43561 tctttcgctt tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc
    43621 atgggagaat aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc
    43681 caactgatag gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac
    43741 gccttgatat ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt
    43801 ccgaatctgg atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc
    43861 gatcaccaga tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc
    43921 ttggagaact ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc
    43981 tacaaactga cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc
    44041 cattggaata tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc
    44101 agtgacaaat tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg
    44161 cagtttcact ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc
    44221 tcgttccata gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc
    44281 aaagtttttt cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag
    44341 acaaaaagga gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac
    44401 aaagcaagag tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt
    44461 acagactaat atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct
    44521 acggatccgt ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg
    44581 gcgtctcgaa acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa
    44641 acgtctgcct cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc
    44701 ttcattaatc caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg
    44761 acttttagct attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga
    44821 ggtaagggga tgcatctgtg ggaaaaattg cttttataaa ttttttaaaa cgtttttctt
    44881 taccttatcg tcctgctaag aacaagatcc aacgtatgct tgaagctaaa aattagaaac
    44941 ctttcatttt cacaaggcac ttgagttcat ttttttactt ttttctgtta agttatagga
    45001 tgtgaaagaa tctagtcact agttgtatag aatctatttt gggggagatt catatgatcg
    45061 aaatctattt atttgttaat cctttagggg aatactgttt cgaaatggaa caacagttat
    45121 tacaatttat cgaagaagag tacgggacac catcaaaaga aaagatgcaa tttcgctttc
    45181 ttcctttggt caatttgcag acaattgggg atgtcatgca acaaaaagga atttctcaga
    45241 acaatcttga ggaacgtaat cacttattct caatgactta ttctgcagca ttggactgca
    45301 aagcggcgca gtttcaagga aagaaaaaag gccgccgttt tttgatcaaa ttgcaagaag
    45361 cagtcggctg taatgacata ccatattctc aagaacttgc tgaatccata tttgaagaaa
    45421 tcggcggcga tttgaatatg tttaaagaag atcgtcagtc tgacttcgtt aaagaagtat
    45481 tcttttctga tcaaaaaatt gctcgggaaa tgggcatcac aaaacaccct tctgcagtcg
    45541 tttacaatta tgcctgcgac cgtgattttg gtgttttagt agaagtaaaa ggtacaagta
    45601 gcctttggag aatcacagag ctgtgtaaaa caacagataa ctcatatcaa gtttttaatg
    45661 gtgaacattt acatacaaat gattctcagt tccattcaag aactagccat ttacacttgc
    45721 tttctaatta aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt
    45781 ctattcgaga aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca
    45841 tttagacgtt gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca
    45901 actcctgctt ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc
    45961 aagtagttct ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca
    46021 gcagaaaagc cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt
    46081 gaccattcga atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc
    46141 aatttaccat aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc
    46201 gtatgcatga aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac
    46261 ccatccaagt aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat
    46321 aaatactccg ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag
    46381 tagtcgccgt acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc
    46441 atttcgtgga caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat
    46501 ggatttgtgt catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca
    46561 acatcgatcc aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt
    46621 ggtttcagtg cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct
    46681 tcccctaaga ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt
    46741 ttccgtaatt ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa
    46801 gtatcgtata cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg
    46861 taattacgga ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca
    46921 aatgtatttc taaattgttc gtaaacactg tacaatccct taaatgctgc ttcgcgcacc
    46981 cttcgatccg tgctttctag caactgacca tacacgccat gagataattg gactatttca
    47041 ccattttctc cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta
    47101 tcacttgaag catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat
    47161 aagacatggg ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt
    47221 ggttcttctt tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca
    47281 aaccaggaaa cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct
    47341 tggtaatctg tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact
    47401 tcagtttggc gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct
    47461 tgatcaagtg ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat
    47521 ttctcatcaa actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca
    47581 ggcaattctt ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc
    47641 ttagctctat ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt
    47701 ttctactcat tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat
    47761 agaaaaccga ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat
    47821 gcattcgtta aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc
    47881 ccttttatgt tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc
    47941 caaggcttct tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa
    48001 ccgacttttt tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca
    48061 ttgttcttgt atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt
    48121 cttcccatct tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact
    48181 agtgaatatt tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat
    48241 atgccctgat acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc
    48301 agctttccag caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa
    48361 tgagaattgc gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt
    48421 attcaaataa ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat
    48481 ttctattgct agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc
    48541 ttccaaaaat actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg
    48601 taagtgctct tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc
    48661 aaaatgagca cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca
    48721 ataatatttc tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat
    48781 tgcaagcagc atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa
    48841 aaagagggcg tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt
    48901 tcccaatatt tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt
    48961 atacgaaaaa aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta
    49021 tacaaaataa aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc
    49081 attcaatgtt tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg
    49141 attctctaca tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg
    49201 ctcttcgaat aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg
    49261 gatcacttgt tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc
    49321 gcttccagat aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact
    49381 cacatcttct gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt
    49441 tttactgata aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc
    49501 gcttcctcgg aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc
    49561 gatttctttg tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc
    49621 atgaccaatt agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca
    49681 cctcactttg ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa
    49741 gaaaaaaaat ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa
    49801 gctatcttat ttaactataa catttttggc agacgaatac tatatagccg acttttcagc
    49861 taattttccc tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta
    49921 atcaagaata ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagt
//
LOCUS       DAZKMB010000008        44874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000008 DAZKMB010000000
VERSION     DAZKMB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.008"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1054)
                     /locus_tag="ACG8V3_000428"
     CDS             complement(44..1054)
                     /locus_tag="ACG8V3_000428"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0298996.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1234..1578)
                     /locus_tag="ACG8V3_000429"
     CDS             complement(1234..1578)
                     /locus_tag="ACG8V3_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0298997.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1571..2005)
                     /locus_tag="ACG8V3_000430"
     CDS             complement(1571..2005)
                     /locus_tag="ACG8V3_000430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0298998.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2143..2880
                     /locus_tag="ACG8V3_000431"
     CDS             2143..2880
                     /locus_tag="ACG8V3_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0298999.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2873..4084
                     /locus_tag="ACG8V3_000432"
     CDS             2873..4084
                     /locus_tag="ACG8V3_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0299000.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4288..5070
                     /locus_tag="ACG8V3_000433"
     CDS             4288..5070
                     /locus_tag="ACG8V3_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0299001.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5140..5802
                     /gene="trmB"
                     /locus_tag="ACG8V3_000434"
     CDS             5140..5802
                     /gene="trmB"
                     /locus_tag="ACG8V3_000434"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0299002.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5900..7654)
                     /gene="ade"
                     /locus_tag="ACG8V3_000435"
     CDS             complement(5900..7654)
                     /gene="ade"
                     /locus_tag="ACG8V3_000435"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0299003.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7786..8715
                     /gene="comGA"
                     /locus_tag="ACG8V3_000436"
     CDS             7786..8715
                     /gene="comGA"
                     /locus_tag="ACG8V3_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0299004.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8672..9721
                     /gene="comGB"
                     /locus_tag="ACG8V3_000437"
     CDS             8672..9721
                     /gene="comGB"
                     /locus_tag="ACG8V3_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0299005.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9718..9996
                     /gene="comGC"
                     /locus_tag="ACG8V3_000438"
     CDS             9718..9996
                     /gene="comGC"
                     /locus_tag="ACG8V3_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0299006.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9971..10441
                     /gene="comGD"
                     /locus_tag="ACG8V3_000439"
     CDS             9971..10441
                     /gene="comGD"
                     /locus_tag="ACG8V3_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0299007.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10413..10760
                     /locus_tag="ACG8V3_000440"
     CDS             10413..10760
                     /locus_tag="ACG8V3_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299008.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10723..11148
                     /gene="comGF"
                     /locus_tag="ACG8V3_000441"
     CDS             10723..11148
                     /gene="comGF"
                     /locus_tag="ACG8V3_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0299009.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11120..11479
                     /gene="comGG"
                     /locus_tag="ACG8V3_000442"
     CDS             11120..11479
                     /gene="comGG"
                     /locus_tag="ACG8V3_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0299010.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11651..12658
                     /locus_tag="ACG8V3_000443"
     CDS             11651..12658
                     /locus_tag="ACG8V3_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0299011.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12683..13867
                     /locus_tag="ACG8V3_000444"
     CDS             12683..13867
                     /locus_tag="ACG8V3_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0299012.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14304..15887)
                     /gene="abc-f"
                     /locus_tag="ACG8V3_000445"
     CDS             complement(14304..15887)
                     /gene="abc-f"
                     /locus_tag="ACG8V3_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0299013.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15992..16120)
                     /locus_tag="ACG8V3_000446"
     CDS             complement(15992..16120)
                     /locus_tag="ACG8V3_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299014.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16465..16608)
                     /locus_tag="ACG8V3_000447"
     CDS             complement(16465..16608)
                     /locus_tag="ACG8V3_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0299015.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16831..17310)
                     /locus_tag="ACG8V3_000448"
     CDS             complement(16831..17310)
                     /locus_tag="ACG8V3_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0299016.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17383..17667)
                     /locus_tag="ACG8V3_000449"
     CDS             complement(17383..17667)
                     /locus_tag="ACG8V3_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299017.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17745..17939)
                     /gene="ssrS"
                     /locus_tag="ACG8V3_000450"
     ncRNA           complement(17745..17939)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8V3_000450"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(18011..19297)
                     /locus_tag="ACG8V3_000451"
     CDS             complement(18011..19297)
                     /locus_tag="ACG8V3_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0299018.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19435..20106
                     /locus_tag="ACG8V3_000452"
     CDS             19435..20106
                     /locus_tag="ACG8V3_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0299019.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20072..20560
                     /locus_tag="ACG8V3_000453"
     CDS             20072..20560
                     /locus_tag="ACG8V3_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0299020.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20573..21520
                     /gene="prmA"
                     /locus_tag="ACG8V3_000454"
     CDS             20573..21520
                     /gene="prmA"
                     /locus_tag="ACG8V3_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0299021.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21521..22285
                     /locus_tag="ACG8V3_000455"
     CDS             21521..22285
                     /locus_tag="ACG8V3_000455"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0299022.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22518..24731
                     /locus_tag="ACG8V3_000456"
     CDS             22518..24731
                     /locus_tag="ACG8V3_000456"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0299023.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24742..25188
                     /gene="dtd"
                     /locus_tag="ACG8V3_000457"
     CDS             24742..25188
                     /gene="dtd"
                     /locus_tag="ACG8V3_000457"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0299024.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25359..26618
                     /locus_tag="ACG8V3_000458"
     CDS             25359..26618
                     /locus_tag="ACG8V3_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0299025.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26707..27654)
                     /locus_tag="ACG8V3_000459"
     CDS             complement(26707..27654)
                     /locus_tag="ACG8V3_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0299026.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28048..29340
                     /gene="hisS"
                     /locus_tag="ACG8V3_000460"
     CDS             28048..29340
                     /gene="hisS"
                     /locus_tag="ACG8V3_000460"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0299027.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29355..31124
                     /gene="aspS"
                     /locus_tag="ACG8V3_000461"
     CDS             29355..31124
                     /gene="aspS"
                     /locus_tag="ACG8V3_000461"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0299028.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31233..32108
                     /locus_tag="ACG8V3_000462"
     CDS             31233..32108
                     /locus_tag="ACG8V3_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0299029.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32374..34257
                     /locus_tag="ACG8V3_000463"
     CDS             32374..34257
                     /locus_tag="ACG8V3_000463"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0299030.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34332..34616)
                     /locus_tag="ACG8V3_000464"
     CDS             complement(34332..34616)
                     /locus_tag="ACG8V3_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0299031.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34606..35340)
                     /locus_tag="ACG8V3_000465"
     CDS             complement(34606..35340)
                     /locus_tag="ACG8V3_000465"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0299032.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35469..36092
                     /locus_tag="ACG8V3_000466"
     CDS             35469..36092
                     /locus_tag="ACG8V3_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0299033.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36284..37405
                     /locus_tag="ACG8V3_000467"
     CDS             36284..37405
                     /locus_tag="ACG8V3_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0299034.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37412..40540
                     /locus_tag="ACG8V3_000468"
     CDS             37412..40540
                     /locus_tag="ACG8V3_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0299035.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40559..43753
                     /locus_tag="ACG8V3_000469"
     CDS             40559..43753
                     /locus_tag="ACG8V3_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0299036.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43899..44606)
                     /locus_tag="ACG8V3_000470"
     CDS             complement(43899..44606)
                     /locus_tag="ACG8V3_000470"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0299037.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14394 a         7966 c         9413 g        13101 t
ORIGIN      
        1 acaaaaaaag cgagacataa gtcttcgctt tttttgtttt cttttatttt gatgaatcag
       61 ttgtttcttc agttgaagat tcttttgttg aatcagttga ttttgtgtca gaactctttg
      121 ttgaagctgt agtttcactt gagtctttcg tacttgatga agttgttgtg aatgcactca
      181 acacattttc aaaggcatca tctttgattt ttacatttgc atcttttaat tcttcaccga
      241 ttactttagt tgtgaatgca ttgtcactta gtttagtttc agtagcgata tcttttagtt
      301 gatctttgta tttatccatg tcattgcctt tattttggtt tttcaccatt ttgacaacgt
      361 agtattctgt cgcataagaa gtaggatttg ttgtagtaat aacatcggag atctcaccgt
      421 ctttcaattt gaaagctgct tcttttactt ctgcaggaat agttgttgtc gttgagtcga
      481 atttaacttt accgccatct tcttttgtct ctgtatcagt tgatttgtct ttggctaatt
      541 tagagaaatc atctccatca tccgctgatt ttttcacatc ttttgcttca tcttcacttg
      601 ataatttgat gatttgtgct tctacttctg gatggaaaga tttccaagca gttttcaaat
      661 cgtcatctgt aatatctacg tgagctttca agccagcttc aaaagctaaa ttgttacgaa
      721 tatattcttt ataagtatct ttcgtatatc cagcagcttc taattgtgat tcaaaagtat
      781 cacctaatga tttcgcttgt ttgtcgtatt ctgcatccac ttgcttatcg gttactttat
      841 cgccatattt gttattgaat actttataaa tgatcatccg ttgtactaag gattggttcg
      901 atgattcaag ttttgcttca tcatagaaat cagatacagt gatcgttccg cctttcatcg
      961 ttgcgatatc tttattagta tcgcctgaac aagctgctag agttaaaaca gccaacgcac
     1021 tagtagctgc caaaataatt gatttttttt tcattaaaat ttgcactcct gtaattagtt
     1081 attattttta tatattaact ataccatatc ccatttcctt taaaaacgtt gtcgtggtag
     1141 tttcaaacaa aattcaaaat ttcatcacat tcctaggaaa tacgcaaact tttctcactt
     1201 atatagttaa atctgttgag aaaataatca ttattatatt tgttcaggat actctggcag
     1261 atcagctttt agtttttcta cttgttcatt gatttgtgtg atacgtggtt cggcttggaa
     1321 tttaaaatct tcgatatctt ttttgattgc ttcttggaaa gcaggaatca cagattcagc
     1381 tgtttctttt gtttcgatca atgcgctttt aaaatgattc aggctgttat ttaaatcatt
     1441 tgtcagagtt gttgcttctt ccaattcttc gatcagtttc tttcttgttt cattcccgct
     1501 cctgggcgcc aataacagac ctccgacagt gccagcagca gctccaaata acagtccttt
     1561 gaaaaagttt ttaatcatcg caggacacct gctttacgat tgtttcttga atagctttta
     1621 gttcttccga tccataacta tcttgatgat tgacaaagtg aatagaaaaa tcatcctctt
     1681 tgctgtagcg agggactaaa tggatatgtg aatgaaaaac agattgatag gctagttcac
     1741 ggttgttatt caagatatta agacctttca tttctggaaa agctttttcc aatgcttgag
     1801 caatcttagg cacacgcgaa aaaacctctc ctgcaagttc gggatcatat tcaaaaatat
     1861 ctgctacatg acgtttaggc acaactaatg tgtgtccttt cgttgcttga gagatgtcta
     1921 aaaaagcata gaccttgtcg tcttcatata ttttatagct tgggatttct tgattgatga
     1981 ttttgcaaaa aatacagttt tccatataaa acatcctttc tataaagaat aaatacttca
     2041 gcttaacttt accataagtt aaagctattg taaggaaaaa aacaaaatac cgtcattttc
     2101 ttcgtggtat aataaataaa aataaatggc ggaggaaaaa gaatgagttt aattatcgat
     2161 catgtcacag gggggtacgg acatctccct gtgttaaaag atatcagttt cgatgtacag
     2221 cctggagaga tagtagggtt gattggactg aacggagcag ggaaaagtac gaccatcaag
     2281 cacattatcg gtctgctgga agcttcaaaa ggaacgatag aaatcaatca tctatcctta
     2341 aagaaaaata cggaagaata tcgcaaacaa attgggtata ttccagagac tccttcttta
     2401 tatgaagaat tgacattgag agaacatatc gaagtcactg cgatggccta cgatatacct
     2461 atggatatag caatgaaaag agcagaaaag ttgttggaga cttttcggtt atctaatcgt
     2521 cttgactggt tcccagctaa tttttcaaag ggaatgaaac agaaagtgat gatcttatgc
     2581 gcctttttga tcgagccttc tttatatatc atcgacgaac catttttagg cttggatcca
     2641 ctagctatca atgcattgct tgaattgatg gtagaaatga aaaaagaagg agccggtatt
     2701 ttgatgtcta cgcatatttt agctactgca gaaaaatatt gtgatcgttt tgtggtctta
     2761 cataacggag agatacgagc agacggttct cttgagatgt tgagaaaaga atttcagctg
     2821 ccggattctt ctttggacga aatctatatt gctttaacaa aggagcaggc acatgagtag
     2881 tttttttcaa aaacgtttag caagacatca aaagaaaatg atgaaatata tgcgttatgt
     2941 attgaacgat cattttgttt tagtttgtct tttcttgatt ggcggtgtgg gattatatta
     3001 ttctaattgg ttgaaaaccc tgcagccacc gtttgcaatt ggagggctca taatctctgt
     3061 attttggatg ctctgtttat tcgtgggtaa gatggctact tttgcagagg cagcagatat
     3121 tgttttttta cttcctaaag aaaaagaaat gcaaacctat ttggaacgag cttttcgcta
     3181 ttcttgcatt tttccgttga tctttttgat attggcatgc ggattggcca tgccattagt
     3241 ggtggttagt acggagcaat ccttttcgat gtttttcgtc tatctgatca tattatggag
     3301 tttgaaattc agtcatctgc aaatcaagag agcagaattg tttcgactag atcaacaagt
     3361 tattcgttat tggaaattag gctggttttt ttcttctctg atctttttgt ttctatcatt
     3421 ttattggcta atatgggcag gaatagcagg tgcattagta caggcaagtc tatattactg
     3481 gtttttgtgg aagcaacaag aaaaaggatt ggattgggag aaaatggtgc atgtagaaga
     3541 acataggttg catcagatct atcaatttat caatttattc acagatgttc ctgaaattac
     3601 ggcaaagatc aaaagaagac gctatttgga tcctatattg actcatatta aaagagaatc
     3661 aaaaaacact tatctttact tgtttgcaag aagattggca agaggttctg attttagtgg
     3721 tctttatttc cgattagtaa ttataggtgg gttattgatt gccggagtag tcgactttta
     3781 ttttagtctg attatcggtt tcttattttt atatttgatc ggtttccaac tgttaccttt
     3841 agcgaaccag ttccgctata tggttctcgt tcagctttat ccaatccata agaatcagaa
     3901 gaagcaggcg attcagtatc tactcacatg ggtattaatt atcgcgtcgg ttatttttgg
     3961 tttgattgct gcagtcgtat tagatgggca gcagcgatgg gttccgctag cggcttattt
     4021 gattgttact atgctctttg ttaaactata tgtaccaact cgtttgaaga agatgtcaga
     4081 ctgaaaacaa agctgtgtag ggtaaaaatg agaatgaaaa atagagcgtt gacagtaaga
     4141 atgctgttcg ttctattttt tttgagaaaa gctggttttt tgaatggcaa ttaaatgaaa
     4201 aagaacgtat atgtgcttga cgtgtagtgt tcaggcgact agaataataa gtactgcaaa
     4261 acaaagagta agaggacggg agaatgcatg atggagtttc agttagacca agagtggcgg
     4321 ttacgtccca tcaaaggcga cacaggaaaa acatacatag gcatgcgtga tgaggacaaa
     4381 gtctttatta aacgaaacac gactccgatg ttggctgctc tttcaaggga aggaattaca
     4441 ccaaaacttg tttggacaaa acgaacgggg aatggcgata ctttgacagc acaagagtgg
     4501 ctggaaggtc gcttgctgac tcctaatgag attggtcaaa gaaacgacgt gattgatgtt
     4561 ttgtaccatt tacatcattc aaattctctt aaaaccatgc tgacccgtat cggtggtcgt
     4621 gtcatgacgc cggagaagat gctgcaaaaa tatgaaaaag aattaccgga agaactccag
     4681 aaaaatcaat ttctgcaaat tgtccatcgt tttcttcgtg agcagatccc gtcaatagat
     4741 aaggaaaact tatcagtagt acacggagat gtaaaccatc gtaattggtt agtatgtaaa
     4801 aattatttat atctagtcga ttgggattca atcatgtttg cagaccctgc ggtggatatt
     4861 gcaacgattt taggaaatta tgtgccactg tctagctgga atcagtggct gatcagttat
     4921 ggcatacgcc cgactgacga aatgcttgaa aaatttcatt ggtatgcggc catgaatttg
     4981 ctccaagaaa tcaccagata ttgcaaacgt gaagatcatc gacggatgaa cgaagaaatt
     5041 ttacaattaa aacgaatttt cagtggctga ggtttggccc aaactctgcg gagatttggc
     5101 cagacctttt taattagagg aaaggaatgg agataaataa tgcgtgtccg aaatcgtccg
     5161 ggagcagcgg aaatgttagc ggctcacccg aattttgtaa ttagtgatcc aacattgtgg
     5221 aagggaaaat ggaatgaatt gtttgaaaac gatcatccaa tccacattga gattggtatg
     5281 gggaaaggcc agttcatcac tggaatggca aaagcccatc ctgaaatcaa ttatattgga
     5341 gtagaaatgc aggtcagtgt tgtttcgatt gcactagaca agttgatcga acaaccgctt
     5401 cctaacctga aacttctaca tgtggatgga tcagcgttga cagagtattt cgctgacagc
     5461 gaagtagatc aaatctatct gaatttttcc gatccatggc caaaaaaacg tcatgaaaaa
     5521 cggcgattga catataagac ttttcttgct gtagatgaac agattcttcg tccaaatgga
     5581 gaaatccatt tcaaaacaga taatcaagga ttgtttgagt attctctggc aagtttttct
     5641 caatatggca tgatcctcaa acaggtatgg ttagatttac atcaaagtca atttgaaggc
     5701 aatattatga cggagtatga agaaaaattt tcttcaaaag gtcaacgtat ttatcgagta
     5761 gaagctcaat ttcaagataa gaccaaaaag aaaagtctgt gaaactaact ttctgctttc
     5821 ataaaaacat taatatccat aaataaatcc cagaaaatga acattacaga tgtaatgtat
     5881 tttctgggat ttatttgatt taaaatgtat ttatttcatc ggtttttcca caaggtggaa
     5941 tcaattggaa gcgagaaaat gaaaataatc ctttgtcggt gatcttcaat tcgggtatga
     6001 cagataatgt taagaaagaa agtgtcaaaa aaggatcaaa ggttttagct gctcctagtt
     6061 gatgtgcttt agttattaat tcattcaact gttcatagac gatcgaaaaa ggttcctgtg
     6121 tcatgattcc gcctatagga agagaaacac aagctatttc ctgctgtttg ttgacaacgg
     6181 tcatcccgcc acctttttga atcaaaagat tagcagcata cagcatatcc tcgtcgtttg
     6241 tcccaacgac aactagatta tgcgagtcat gggcaatcgt tgtcgctatc gctccttgtt
     6301 tcagctgaaa tcctttcact atgcctaatc caatgtttcc cgtttgatgg tgacgctcga
     6361 tcaccgcaat ctttaattgg tctttttcga ttgaaggaat gaatcgttcg ccttgtcggt
     6421 ccactttttc taccaaatct ttagtcacta gactattcgg gataatttcg atcacatggg
     6481 cttgtgaaga agaaagaagc agctctaacg aatgtggatt taatggttga gcgtttagtt
     6541 ttggcagaca acacgtatat gaatttgaat gatcagcaaa taaatggtct tggagttgcc
     6601 cagattgaac aactaatgtt cccttataga aaacttcatg aattggcagc ttggtaagat
     6661 cgtctgttaa gaagaaatca gcttgatacc caggggcaat cgcacctagt gttttcaaat
     6721 caaaacattc tgctgcattc aatgttgcca tctgaatggc ttgtaatgga tcgattccaa
     6781 gtgcaatagc ttgacggacg atatgatcga ttgatccttc ttttactaaa tcattgatca
     6841 gtttatcatc ggtgacaaag aagcatcttc ttgaattttc aggtgtgatc gccggtagta
     6901 gcgatgccag atcttttgca acggtccctt ctcgaaccat taaatacata ccaagatcca
     6961 aacgttcttt agcttctttt gcagttgtag cttcatgatc cgtccgaata cctgctgcag
     7021 gataaacatt taactcttcc atgccgatac cagctgcatg cccgtcaatt tttttcttat
     7081 gctggtgcat caattggatc ttttctagga tgtctgtttc attatttgct actgcttgat
     7141 agttcatgac ttcagctaag cctaaaactt cgggtttttg taaaaaaggg gctaattttt
     7201 ttgcatttaa atttgcacca ttggtctcaa aaggggttac aggtacacaa gaaggtagca
     7261 taaaaaagat attcatgagt gtttgtcttg catcttctat cataaactca agacctgtac
     7321 tcccagcaat gtttcctatt tcatggggat ctgtcactac agtagtaact ccatgaagca
     7381 aggatacttt tgaaaattcg cttggtgata aaagtgagct ttcgatatgc acatggccat
     7441 cgatgaatcc tggcactaga tattgcccat tagcattatg tacgcttttt ccttcgtatt
     7501 caccgattcc tacgatcatt ccttcgcaga ttgctacatc tttttccatg atttctttcg
     7561 taaagacgtt gacgatctta gcatgcttga tcactaggtc agcaggcttt tttttacttg
     7621 ctacttcgat atatttcctt aatgttgttt ccaatggagt gcctcccaag ataaattttt
     7681 ataagtataa cataaattcg gtttttgcca atattttttt gtaaattcat tttatttgaa
     7741 acaaaaagat caaaaagacg tatgtttttt gtggagggga gaataatgga aataaaacga
     7801 ttatcggatc aaatgataag tgaaagtttt gccaaacatg cccaagacgt atatatattg
     7861 ccagcttcag aagtatttta catctatttt cgctatggaa aaaaacgaac attctggaag
     7921 aaagtctctt cggcagatgg acagcaactg atctcaagat ttaagtattt gggacagatg
     7981 gatatcggag aaaaaagaaa agctcagtta ggtgcattta cttattcttt agaaaaacag
     8041 gaagttcgtc tgcggttgtc ttctgtagga gattaccttc aacgagagag tttagtgatt
     8101 cggctattat acggtacttt tgaaacaccc catcgttgct ttagttctcg agattttgca
     8161 actattttac agaaaaccgt cagacgtgga ttataccttt tcagcggacc agtcggatca
     8221 ggcaagactt ctctaatgta tcggttggct ttggaagaaa aattgcaagt gattactatc
     8281 gaagatcctg tagagataga agaaccgttt tttctgcagc tacagatcaa tccgaaaata
     8341 gcacagactt atgatacact tttgagactc gcattacggc atcgtccaga cttgctaatc
     8401 attggcgaga taagagatga atcaactgca catgctgcgg taagagcagc actaacaggg
     8461 caccgggttt ttgcaaccgt acatgcacgc aatcttgaag gtacattggt cagaatccat
     8521 gaactgacaa agaaaaaaga agagctatct gaatgtttag ctggtgtcat ttatcaagaa
     8581 cttttggaag atgccaatca ccatccttct gttttatgga gttatcaatt tatggaggaa
     8641 gaatcagtaa aaaaatcatg ggaggaaagt tatgaagaag ctcaggaaaa tatttggcag
     8701 tcagaactat tctaaaggat taacaagaag ccagcaaaac cagtttgcac aaaccatggg
     8761 agaactgctt ttatctggtt ttagtctgca agaatccctt tatttattac tcacgatccg
     8821 ttcgtttcca cctgttattt tggcaaatat ccaacatcag ctaaaggaag ggcaagcgtt
     8881 tagtgaggta ctttcgcaaa taggatatgg agcagagcaa ctggtacaga tcgagttagc
     8941 tgaacgtcat gggaatctgc cgacaacttt gttggaaatt gccaaacagt ttcggcttgt
     9001 tgaaggattt cgcaatgatt tgaaaaagct gcttgcatat ccgatgctat tgctcacctt
     9061 tttgctaggt atcttgttta gtatgcggtt gttcgttttg cctcagctta ttggttcagg
     9121 aatggtcatg caagagcatt ggggtattca gctgatacaa gtctttcatt ggtatgttct
     9181 actattcatc ctagcggtta ctctattggc tttactagta aggtatcgtc tgatgaagtt
     9241 gtctcctgtt gatcagagtg aatggataag cagacaatta ttgattggac cgatatattc
     9301 cttgtatcaa tcggcttatt tgtcactaga gattggaaag ctttttcatg aaggtttcga
     9361 attaaggcaa atcatagtct gcttaaagga aacgaaacaa gaaagtctga ttcagcatct
     9421 tgcctctaag ttgattcaag gattggaggc aggtatgtca ttggctgagc agttccagaa
     9481 ttatcgtttt ctatcagaag aattcagtaa aattgtttta cagggagagg caaagggaag
     9541 tttaggtaaa gaactgattt tttacagcga atggacaaga aaacagcttt tcaaacgtct
     9601 tcatcactgg atgcatttta ttcagccaat catttttttg ggcgtagcgg ctttgatttt
     9661 attagtttat gccgctgttt tattacctgt ttacggaagc atagaggagg ttttgccatg
     9721 aaaaagagaa aaatatctta ttatagcggt tttacattga tcgaaatgct aatcgtctta
     9781 ctgatcatca gtgtattagt gttgttgttc gtccctaatt tgtcgcgtta taggaatcat
     9841 gtagaccaag aaagtcgtga agcgatcatc cagttggtag acacacaaaa agagctctat
     9901 gcgcttcaaa ataatggaag agttcctact gtggaggaat tgttgaatga aggctatatc
     9961 aaacgagaac atgcagaaat ctatcagcgg ccttgaaggt aaggttttag catcaggata
    10021 tacgttgttt gaatcactct tactgatttt attacttaca acgatccttt cttttccagt
    10081 tctcgcattt tctgcatgga agaatgaatt ggcagtctat caatttttcg gccaatttga
    10141 aaaacgaatc tattctacgc aaaaaatcgc tattgtgaat cagatccaaa caggctttta
    10201 ttggaatagt gaagagaatc aaatcatttt tcatgttccg aatccagata aaataaattg
    10261 ggaagtatta gatattcctg atgagattac cttgaaacgc catgcttcta ttacttttgc
    10321 tgctagaacc gggaatgaaa gttcactaaa agcttatcaa ttttattggg aagaaaaaga
    10381 gcggacgatt acgtatcaat tccagatagg gagtggtcgc tatacaaaaa gaattgaata
    10441 aaacaggatt tgttttgttg gagtccctta cagcgcttat gatcgcttct gtctgtgtct
    10501 acttagtgag ctttggccag ctgtttttgc ttcaggcaga aaagaaaagt ggggaggaac
    10561 tgctatttat tcgaatgatt tatgaagata tcaagacgca gcggatgtat cacaaaagac
    10621 cagagcgaag ggaagaaatg atcccatacg atatccaaat tgtcgataat aatggagtaa
    10681 tcgaaaaagt gtttgtcaat atggagaaag aagcgatcag tattgaaaaa acaagtaatt
    10741 ccagcattta cgctgattga atgtctaatt ggactgatga tattgagcag tctattgctc
    10801 acattcaatc ttttgattca gcaaaccatc caagtaagtg aatttttgca gcgtaaagat
    10861 caaaaagagt ggctaatatt tttagcacaa ttagaagaag aactcaagag cagtcacaat
    10921 gtctctgtga aaaatcagca actgcactac acgatcgagg ataaacagta tatcatcgaa
    10981 agatatcagg caatgatacg taaaagaaga acttccggcg gacatcaacc tatgttaaca
    11041 agtatcactg agcttcaatt attggaaaaa gaacacacaa tttcttttta tgtacacttt
    11101 gaaaatggag aagatggata tgccatatgg acaaaaaacg atcagtagaa aatgttataa
    11161 gggctctatt ttattttctg tcttgctttt atttctttta tgtacgtttt tattgatggc
    11221 agcattagaa agctaccgtt tgtcaatgga tcttaatata cgaatcaaaa attattatat
    11281 agcaaaaata atgaagcaga tggctttatc ggaacagtct acgcttgccg aaagttcgga
    11341 aggagttttt tactacacaa ctggatctgt aacatatcag tgggtacaac agtccctatt
    11401 cttagaggtg gaagtctcac cgtttatctt tcggttcaca gaagaagtaa aaaatgatac
    11461 agatactagt acagaatgat ggaatgttcg tgaaaacgcc ctcttttctg ttataacact
    11521 gtttttaata aaaaaatgtt tgccgtttca caaataaaaa acagtgaaaa cgattgaaat
    11581 tttgtcaatt gttcgataga attaaacagg tgaaaaaatt gttataatat atctagaaat
    11641 gaggtgcacc atgttaccag aaaaaatgga acaagggttt aatcaaaacc tggaagctat
    11701 tcagctttta cagaatgcat tgggaacttc ctttttagaa gcctatgtcg aaaatgcaga
    11761 aaatttaata gatgattatc aagtacgtgt ggtggatgga gtgccgacaa aagagacaac
    11821 gcaacggatc acagctcttt atgaagagtt gaagaaactt tctttcgagc cagaagaatg
    11881 gcgcagactg tctcaattac ttttattgaa gggcagccag acagaacatt tgcaggcgaa
    11941 ccatcaactc actcctgata gtatcggttt tttatttgtt tttcttattg aacaactata
    12001 cacaaataaa aaagcaccgg ttaagattct ggatattgcg gcaggaatgg gcaacttgtt
    12061 attaacagtt cttttaaacc tatcgaatgc ggggtaccaa acagaaggaa ttggtgtgga
    12121 tattgatgat acattgttgg ctgtcgcagc ttctacaagt gatttgacac aagcaaatgt
    12181 tcaatacttt catcaagacg ggttgcagga attattaata gatcctgtcg attttgcaat
    12241 cagcgatttg ccaatcggtt attatccgaa tgacgaaaaa gcaaaagagt ttttaacaag
    12301 taccgaagaa ggccatagtt atgcacatca tttattgcta gagcaatcaa tgaaatatgt
    12361 gaagccggat ggttttggtt tgtttttgat gccgagtgga tttttagaaa cggatcaaag
    12421 tgaagaaatc aaaaaatggt ttaaagaaga aggttatctt caaggaatga ttcaattgcc
    12481 agatgaattg ttccgtaata aacaatcgca aaaaagtatt ttgatcttac agaaaaaagg
    12541 accacaagca caacaagtaa aagaagtctt gcttgtgaaa cttgcttcat taaaagaacc
    12601 agagaaggtg acggaatttt tcaacgagtt taaaaactgg aaatcatctg ctctgtaatc
    12661 attcaacgag aaggagagtc atatgtctaa aacaattgca atcaatgctg ggagttcaag
    12721 tttgaaatgg caattatatc aaatgccaga agaaaccgta gttgcgaaag ggatcgtcga
    12781 acgtatcggt ttacaagatt ctatttttac catcaagtac ggcgataatc aaaaattcga
    12841 acaaatcatg gatatcgaaa accacgaagt agcagtaaaa atgctgttag atcagttgat
    12901 tgaactgcaa attttaagct cttatgatga aatcaccgga gtagggcacc gtgttgttca
    12961 cggaggagaa acttatgggg attcagtagt gatcgatgag aaagtaatgg accgcattgc
    13021 tgaattagca gaatttgctc ctttgcataa tcctgcaaac ttaatgggga tcaaagcgtt
    13081 taaaaagatt ttacctgaca ttttgagtgt ggctgtattc gatacatctt tccactcaac
    13141 aatgccaaag caaaattatt tatatagctt accggtgtct tattatgaag atttcaaagt
    13201 aagaaaatat ggcttccatg gcacaagtca tcgttatgtg tcggaacgtg cggcaaaatt
    13261 attgaacaaa ccgatcgaag aactaaaaat catcacttgc cacttaggaa atggtgtatc
    13321 gatcacagca gtcgatggag gtaaatccat ggatacttct atgggattca caccattagc
    13381 tggggtgaca atgggtacac gttcaggtga tatcgatcca gctgttctcc catatttaat
    13441 ggaaaaatta gagattgata ttgatgaaat gatcaatgtt ttgaacaaga aatctggtct
    13501 gctaggccta acaggattgt ctagtgatat gcgtgatctt gaaaagaact atgaaaaaga
    13561 acatattcgt ttagcttatg acattttcgt ggaccgcatc cgaaaataca tcggcggtta
    13621 tgtgacaacg atgaatggtg ttgatgcaat tgtctttact gctggtatcg gcgagaatga
    13681 cggacatgta cgtagtgaaa tcatcaaagg tatgtcttgg tttggttgtg aaatcgatcc
    13741 agcgttgaat gaattgcgtg gtgaagaagt agatatctct acaaaagaat caaaagtaag
    13801 agtattagtg atcccaacag atgaggaact aatgattgcc cgtgatgtgg agcgtctgcg
    13861 cggctaagcc aaagcattcg ttaggataaa agatgaaacg catgcaagag ggaattcttg
    13921 catgtgtttt ttttgcggag aagaccgcaa aatggtaatt taacttcaat ttcccttctt
    13981 ttttacagga tcttcttagg gaattttgct tggaaaagtg gtcaaaaggg aaaagactgg
    14041 cattttattt attgattttc aggaaaatcc caatatttta ttttcattca tctttataag
    14101 ggacattaca tatgtaaaaa gaaaaaacat ttttagatca gcctcgtggg ctgtcgactt
    14161 tattcttcgc tttatgttta gctagaaaac cgatccgaaa cattcattga tcgaataata
    14221 aaaacttgat tttccacctg tactttgcct gaatcgtgaa agcacaggta tgaaaatcaa
    14281 gttttttgtt gcgaaaaaac gtcctagtca atcagttggc cgagcggctg gcggctgatc
    14341 tgctcctgat caataacgag tgcttcttgg aagtgttgct gcaaaaagta gcggtcatgg
    14401 ctgactgcta ggagcgttcc ttgatagtct tgcagcattt cttcaatttc ttctcgagca
    14461 tagctgtcga ggtgattggt tggttcatcc aaaatcagga cgttgatttt ttctaaaaag
    14521 agtttcagca acgccaaacg gattttttca ccaccggata aatctttgat ccgtttaccc
    14581 acatcgcttt gataaaagcc acttttggct aattgccgtc gagcgacttc ttcgtttcca
    14641 gtcaatgtga tggcgtaagc cagcaaacgg ctatcgggat cggcaaactg cagctgctgc
    14701 ggcaaataac cgatttgcaa gctactacct aatttgatgg tgccaccgta aggcttttcc
    14761 tcaccgagta ataatttgag taaggttgat tttcctgcac cattgtcgcc gatgatggct
    14821 aaatgatctc cacgatagac tgcaaaactg ctgtctttga atagtacttg atcagcgtac
    14881 tgctggtgca agttttgaat gatgaaaact tctttgccag attttccacc attagacaag
    14941 ctttgcagcc gatttttcgg gggaggtggc ggtttgacga gtgtcagttt cgccaggcgg
    15001 cgttccagtt ctttggcttt tttgaagaag gactcattgt ctccttcatg tgcccactga
    15061 cgaaaacgac gaatcatgac tttcaaccgc tggacttctt tttgctgcag ttcgtaattt
    15121 tttgtcagct cagccaagcg agcttctttc aagactcgaa aacggctgta gtttccggga
    15181 tactcaatca atgcaccttc ttccagttcc catgtttttg tggtgacttg atcgagaaat
    15241 tgccgatcat gagaaacgac cagaaccgat ttttttgtat tctgtaaata gttttccaac
    15301 cagcgaatac tgttaagatc taaatgattg gtcggttcat cgagtaaaag taaatcggca
    15361 tcggctgcta aaattttggc taaagcgact ttaactcgtt cgcctccgct aagttctgtt
    15421 aaccgtgctt cctgacggtg tggaatccct aagccttgca agatcgctgc aatccggtct
    15481 tctaatacgt aaccgcctag ttcttcaaag cgatgctgca gctcaccgta acgggccaga
    15541 cacttctcta agtttgtatc tggttctgtc atcaatgctt cgtagcgact catttgctgt
    15601 ttgaggtcct gtatctcagc ggcagaatgg agcaagtagt ggttgacggt cgcttcggaa
    15661 actgtaagtt cttgttcgac cgtcccgatc tttaagcctt ttttgcgact gataacaccg
    15721 ctgtcgacac cttctctgcc ggtcaagatt tgtaaaaagg ttgatttgcc agttccgttg
    15781 ataccgacta aggcaatttt ttcaccagct tcaatttgac aggataaatc ctcaaataat
    15841 ggattcccgc cgattgtttt tttaatagaa attgcttgta tgatcatgat ctctctcctt
    15901 gagaccactc aaaaaggcca tggttatcca tggcctgtgt tatttaaact cagattatca
    15961 tgagaaaaat accttgagat ggccattgat tttacgtgaa ttcaactgtt ttggacaaac
    16021 ttgtcccgtc aacagcaact gtaaaaccaa ttgcatggct aaatgcgata tggtatcgtt
    16081 ggtaattttc attacttgtt attttagtca tgcgcatcat agtattttcc ctccttcgtg
    16141 tagttgtctt ttccagtgta cctgaaagtg tcagaaagtt caactagcgc tgacatttac
    16201 ttaatattta tcgatgtgta attctgtcag agagcttttt ttatctgact gagggtaatg
    16261 tctcgtggac cacatcattg tattctgagc taaaccagcg ttcaggcgaa atcatatggt
    16321 gtttgataaa aaactttgtc tacatgactt ccttttgcca tttgcttaat accgttgcat
    16381 taaacagctc ttctctttgg aaatctcacc aacatcgcgt tcaggagtat cgttgtggtt
    16441 gcaatggcaa gcggtagcct ttcatcaatt cttgaatgat ttaccgaatg tagcacttgc
    16501 cttctcgcgg tacccgaacc cgaaataaaa ttttagtttg gcgataaaat cggtatccgc
    16561 catcgatact tcatattttt tcgtatcaga ttcatcggaa acttccacat tgacgaacca
    16621 ataagctttg gcatgcttag gtcgttttaa accacaccag ggcgactaat cgccctggtg
    16681 ttcacttttt tttattttac ttgaactcat tgactgtttt ccctttattt taaacattat
    16741 aaagaccgat gagtgcttgc cgcgtctttt gtttttatca cggttttttg tgcaaaaaaa
    16801 cctgccgata gttggcaggt cgtctacgtg ttaagctttg gtgtggtctt tagctggcaa
    16861 ttggttttct aaatcagttc ggacaacaag cacatcgcaa gctgcgtttc taataacata
    16921 ttcagaaaca gaaccaataa ataaacgttc tacagcattc aatcctgtgg ctcctaacat
    16981 aatcaaatct acttgattat cttctgggat ttctctggca ataatttgtt ttggtgagcc
    17041 atattctacg acagaggtta cattattcaa gccagctttc ttcgcattgc tttcataatc
    17101 tgccaatgtt tgctttgcca tttcagtagc ttgttctgca agcattccat caaaggatga
    17161 aacggtttgg aaagaacgtg tgtcaatgac atgtgctaat agaagttctc cgttatttcg
    17221 gatagctaca ttgaccgcct ttttaaaagc taattcagct tcatcagaac catctacggc
    17281 aaccatgatt tttttgtatt gttgtaacat aagcattcac tcctcaagtc aatcgtattg
    17341 tactctttta ttgtagaaca ctttagggaa aaagagaatt atttactgct ttggttcagc
    17401 aatttttttt gcaaatcgga tactgaaggt ggcagaaaca ataattacga tcagtaaaaa
    17461 gaccaaggca ataaagcgtt gattgaaaaa agctgttaaa atacccatta cgcctaaaac
    17521 agcatatgca gctccgtaaa attgtaggaa tccttgattt ttatcatttt tgttgatcaa
    17581 tacaaaaaag atatcctgtt tattaaataa ataatagcta acgaataata atactaaagt
    17641 ggcgataatc attaaaatcc taatcatgaa caaatcctcc aaaataagca acaaatagaa
    17701 aaagcaattt gccaagagca aattgcttta ttctttaaaa gatggtaatc tccgttaatg
    17761 ccgttgtttg tcctgtagta ttgatcccgc gaattcaagc aggtgggtcc catggtcgta
    17821 gcttcgaact accaaatgat aaggcatgtt accagcagtg acacaagacg ggtcccaaga
    17881 tatcaataaa aatgttcggt caacacacaa agaggacaat tcgaacatca cagatttccc
    17941 acctctatag tagcatatga atttaaaaag agcaatgaga gagctcgcag aaacagaacc
    18001 gttttcttgt ctagtttttt ggaggatgct ttttccactc ttttatacgg taatactgtt
    18061 gacccagagc ctgttcatat ttccctgtat cttttggctg ataatactga gcattcttca
    18121 gtttatctgg taaatattgc tgattgaccc aagcctgatc aaaatgatga ggatactgat
    18181 aacccacgcc acggttgagt gatttcgcac ctttgtaatg gctatctctc aaatgatcag
    18241 gaacatctcc agctttgccc tctcgaatat cagcaagtgc tgcatctaat gccatgtagg
    18301 cagaattgga ttttggcgac aagcaaagat cgacgacgac gtctgctagt gggatacgtg
    18361 cctcaggcaa tcccaatttt tcagcagcta atacagcgtt cactgtacga gccgcagcag
    18421 caggattacc taaacctata tcttcataac cgataaccat cagacgccgg caaatacttg
    18481 ccagatcacc tgcctcaact aaacgggcga gataatgaag tgcagcatcc acatcacttc
    18541 cacgaatcga tttttgaaaa gccgaaataa cgtcataatg agcatctcca tttttgtcat
    18601 gggtaagagc ttttctttgg acacattctt caatgatcga cagtgtaaga tgaatccttc
    18661 cttctttatc tgaaggagtc gatagtgtag ccagttccag cccattcaat gcactccgca
    18721 aatcgccgtt cgttgcacgc gaaaggtgaa gaagagcatc ttcgtctaac tggattgctt
    18781 gttgtccaag gccgcgttct ttatccctta aagcatgttc gactgccaac tggatgtctt
    18841 gttctgttaa tggtttgact tcaaagatct gtgtacggct gcggatcgct ggattgatgg
    18901 taatataagg attctcagta gtcgctccga tcaagatgat tcggccactt tctaaatgag
    18961 gaagcagaaa atcctgtttt gttttatcta atcggtgtac ttcatccaat aagaggatca
    19021 ctgtaccact catttttgct tcttccgcta caacttgtag atcctttttt gaatctgtag
    19081 cggcgtttag catgcgaaat gcataattag tagagccggc gatcgcactt gcgatactcg
    19141 tttttccagt accaggaggt ccatatagaa tcatggaaga gagcatgcga gcatcgacca
    19201 tgcggcgtat gatttttcct tccccgacta gatgctgctg accaacgact tcatcgatcg
    19261 ttcgaggtct catgcgataa gccaatggct gttgcatagg attttcctcc ttcttgtttt
    19321 tacgtccctt aattatatca tatttagcaa gatgcctaga aagaacagcc ttctgttgtt
    19381 ttgttttcat agcttatcca actaatattg tgtatgataa agtaggtgag aaaaatgacg
    19441 gataaacaaa agatagatga atttttttgg aataaaacga cgccagaagt ggcacgggat
    19501 ttattgggga tgtatttgga gcatgacaca ccagaaggaa gattagctgg atatattgta
    19561 gacgcggaag catacctagg tccagaagac gaggccgccc atagctatgg tttaagaaga
    19621 acaccgcgtg tgcgggcaat gtatgaaaaa ccaggtacga tctaccttta cacgatgcat
    19681 actcatcgga ttttgaacat cattactcag ccagaaggga ttcctcaagg agtaatgata
    19741 cgtgcaatcg aacccgccgc aatgattgat caaatgagta aaaatcgtgg cggaaaaact
    19801 ggtccagata tcagcaacgg tccgggaaaa ttagtggaag cattagcaat tcctcaagaa
    19861 ctttatggtc agtctatagc agatagttcg ttacgcttag tttttgagaa aaaaaagaca
    19921 ccaaaaaaga tcaatgcttt gcctagaatc ggtatcccaa acaaaggtgt atggacagaa
    19981 aagccgttgc gttttgttgt gtcgggaaat ccttatatct ctttacaaag aaaaaaccaa
    20041 atcgagaaag attggggttg gaggaaagaa aatgaaaaag aaggatctat taacatattt
    20101 agatgaaata attgaaaaac aagtgacaga ctatgatgta gctttagatt gggatacaaa
    20161 aaatcatacg atcgagatcg ttgttcgctt gtttgcagaa aataaagcac aatttgagat
    20221 tgatgatgca gaagggatcg tttcgcaaga accaattatc gaatttgaag acggcgtact
    20281 tctttttaat cctcagaaat ctgtttttga tgaacgcaat tatctagcag taatccctta
    20341 tgaaggaaaa aaagggttgg caaaatcagt agctgattct ttagtggaat atctaaatga
    20401 agtattagct caagggcaaa gcgatttgct tgattttttg gatgaagacg atgacgaagc
    20461 tgtattcgag cttcactggg ataatcagca actagcagaa ttagtagaag taaaacagca
    20521 aaatgaagca gatacttatt taccgtaccc aagttattaa ggagtgacag atatgaaatg
    20581 gaatgaagta aaagtagaaa ctgaaagtga agcagtcgaa gcagttgcta atattttgat
    20641 ggaggcaggg gcgagtggtg tcgcgattga agattcttta gatgttgaac attttcagtc
    20701 ggatcaatat ggtgagattt tagataaaaa tgattttaca tcgctgaaag aaggcgcact
    20761 agtcatggct tattttccgg aaacgacttt cttaccggaa atcttgcctt ttatcaaaga
    20821 tcgtatcaca caattacctg agtttggtct agctattgga aaaaataaag taacagttag
    20881 tgaagtagag gaaagcaact gggcaacagc ctggaagaaa tactatcatc cggtgcgtat
    20941 tacgcgaatg ctgacggttg cgccaagctg ggaaaactac cagacagaag atcctctaga
    21001 aaaaattatc acactagatc ctggtatggc atttggcaca ggcactcatc ccacgacttc
    21061 cttgacattg acagcactag aaacagtctt gcgtggtggt gaaacattgc tagatgtggg
    21121 aactgggtcg ggcgtgctaa gtattgcagc aaaatatctt ggtgcaaaag atgtctatgc
    21181 ctttgattta gacgaagtag ccgttcgttc ggcgaaagaa aatatggata tgaatgaaat
    21241 agccaaagat gtccatgtat cagctaacga tcttttgaca ggaatcgaga ttgaaagtga
    21301 tgtaatcgtt gcgaacattt tagcagatat catcttattg atgatcccag atgcttggcg
    21361 tttactgaag caaacaggca cgctgatcgt ttcaggaatc attgaagaga agaagcaaat
    21421 ggttctggat gccatgttag aacaagggtt tatcgtggat caaattttcc agcaaaaaga
    21481 ctggtatgca attattttga aaaaaccaga ggtggaatag atgcagcggt attttttaaa
    21541 agaatcatat gaatcaaaag actattttgt tttagaaggg gaaaactatc accacattgt
    21601 acgggttatg aggatggaac ccgaggatga agtttttttg gcattcaatg atcaaatagc
    21661 cattgttgca gaaatcacgg agattactga agaggctgtt tatctgaaag aaattaaaaa
    21721 agaagaaaat caaaaggaac tgccaagttc gattaccata gcgagtggct atcctaaagg
    21781 ggaaaagctg gattggatcg ttcagaaatc cactgaatta ggagcgcaag cttttcttgg
    21841 tttcccggca tcagcctctg tcgttaaatg ggatgcaaaa aaacgtatga aacgacaaca
    21901 acggcttgaa aagattgctc aagaggcagc ggaacaatcc catcggcaga tcgtgccatc
    21961 agtagagtta ttggaaacga aacaagattt atttgagcgg ctagatgatt atgacgtggt
    22021 cttggttgct tatgaggaat cagcaaagca aggcgaaaaa tcaaatgcag ccaaaattct
    22081 tagtgaaaca aaaccagata gtcgaatctt agctgtcttt ggaccagaag gtggattatc
    22141 ccgcgatgaa gtcgattatt ttgtagaaca caaagggata ctttgtggat taggaccaag
    22201 aatccttcgt acagaaacag cacctcttta tttattaagt gctgctagct atcaatggga
    22261 attaatgaat ccttctttca gctaacgttc ttcattaata aaagagtatc gacagaaaga
    22321 attgaaaaaa tagtcaacaa tttgtataat agaaacaatt atttatacaa tggatacgaa
    22381 aaatcgtttg aaaaagcttt taaaaagcga acatttccgt aaaaaaacgt ataactgcac
    22441 ggattttggg acaattatca aacagatatt tgtcccgatt cccgttttac cgaaaaaaga
    22501 aagggggatc gattcctgtg acaaaagaag aaattttaac gggacccggt gtcataaagc
    22561 tcgttagtca atacatgggt cccgaacatg tagcatttgt tgagaaagca tgcgaatacg
    22621 cgactgctgc acatgacgga cagtttagaa aatcaggcga accctacatt attcatccta
    22681 tccaagtcgc aggtatatta gcagatttaa aaatggatcc ccatacagtg gctacaggct
    22741 tcttacatga tgttgttgaa gatacagaaa tcactttaga agatctgaga gaagaatttg
    22801 gcgatgacgt tgctatgtta gtggacggcg taaccaaatt agggaaaata aaatataaat
    22861 cccacgaaga gcagctagca gaaaaccacc gaaagatgct gctagcaatg gctcaagatt
    22921 tacgagttat catggtcaaa ttagctgata gactacataa catgcgtacg ttgaagcacc
    22981 tgcgagaaga taagcaaaga aggatcgctc aggaaacttt agaaatctat gcgcctcttg
    23041 cacatcgtct agggatcagc cggatcaaat gggaattaga agatacagca cttcgttatc
    23101 taaatccaaa acagtattac cgtatcgtcc acttgatgca gacgaagaga gaagaacgtg
    23161 aaaaatacgt aagtggtact gttgaagata ttcgaatagc gacagaagag ctggggattt
    23221 ttgcagaaat ctatggacgg ccaaaacaca tttattcaat ctatcgtaaa atgaaagatc
    23281 agaaaaagca gttcaacgaa atttatgact tgctagctat tcgagtgatc gtagattcaa
    23341 tcaaggattg ttatgctgta ttaggagcaa tccacacaaa atggaaacca atgcccggca
    23401 gatttaagga ttatattgcc atgccaaaag ctaatatgta ccaatctttg catactaccg
    23461 tgatcggtcc ggcaggaaat ccggtagaaa ttcaaatccg aacacaggaa atgcatgaaa
    23521 tcgctgaatt cggggttgct gcacactggg cctataaaga aggaaaaaat gaaaaagtag
    23581 aaccagatgg tatgacgaaa caattaagct ggttccatga gatactcgaa cttcaagacg
    23641 aaagctatga tgcttctgaa tttatggaag gcgtaaaagg agatatcttt agtgataaag
    23701 tctacgtctt cacaccaaaa ggagacgtta ctgagttacc aaaaggatcc ggaccattgg
    23761 actttgcata cagtatccat acagatatcg gtaacaaaac cactggtgca aaagtaaatg
    23821 gcaaaatggt gcagcttgat tacaaattga aaaacggaga tatcattgag atcatgactt
    23881 ctccaaattc atttggccca agtcgcgact ggttgaaatt agttgctact agcaaagcaa
    23941 gaaataagat caaacgtttc ttcaaagccc aagatcgaga agaaaatgtg atcaaaggcc
    24001 acgaatccgt ggtcaaatgt attacagatc taggatttac gcctaaagat attttgacga
    24061 agaacaaact gcaagaagca ctcgatcgtt ttaattatca aacagaagat gatctctatg
    24121 cagctgtagg gtatggagaa gttagcccct tgacgatggc caatcgtctg actgaaaaag
    24181 aacgtaaaga acaaaaaatc gagcagcaaa agcaagaagc agaagaaatc atgaatcagc
    24241 cgaaaaaaga acctgacaaa atgaaagtac gtcatgaagg tggcgttgtc attcaagggg
    24301 tagaaaactt actaattcgt atcagtcgct gctgtaatcc gattcctggt gatgatatcg
    24361 ttggttatat cactaaaggc agagggatat ccattcatcg tcgagattgt ccgaatgttc
    24421 agcctgacaa accaaatgta gcagaacgtt tgattgaagt cgaatgggaa gatacatcga
    24481 atacacgaaa agagtatgat gcagatttgg aaatttacgg ctataatcgt tcaggcttat
    24541 tgaatgatgt acttcaaaca gtcaatgcgc taacgaaaaa tctcaacagc gttgaagcac
    24601 ggacgaataa agataaaatg gcgacgatcc atttgacggt tggtatccag aatttatccc
    24661 acctaaagag tatcgtggat aaaatcaaag cagtacctga tgtctacagt gtacgccgga
    24721 cgaatggata ggaggacaga tatgagagca gttgttcaac gggtaagtaa agctgctgtg
    24781 tcagtcgatc agcaagaaat tggaaaaatt ggctacggcc tacttgtttt attaggcatt
    24841 catgaaaccg atacgcaaaa agatgtggac tatctggtaa aaaaaattgc acaaatgcgt
    24901 atttttgaag atgaacaagg aaaaatgaat attagtgtag aagatgtggg aggacaaatt
    24961 ttaagtatct ctcaattcac actgtttgcg gatacgaaaa aaggaaatcg accaagcttt
    25021 gtggaagccg cacgaccaga aacagctatt cctttatacg aatcattcaa tgaaggaatc
    25081 agaagtcgcg gcatcacagt cgctaccgga caattcggag cagatatgtc ggtttctctc
    25141 gtcaatgatg gacctgtaac gatcattatt gattctaaaa acaaataaag taatctggtt
    25201 ttcactagta aaaactatga aacacgtcgc ttaagttaga ctgataaact ctaacttaag
    25261 tggctttttt gtctgcaaaa agcgcttctt ttgtcagcag tcggaaaaaa agttaagctt
    25321 aaattataca agttattttt tataggaggt tgctgaagat gaaagaagta ccagtagtag
    25381 atatcaaaat cacagattta aaaaaattgt atcaaaaagg atacaacatg ttggaggagt
    25441 tacgccatga agcggacgct cccgtcgtca aagcaaagat tttcaataaa gaagctatca
    25501 cgatctacgg atcatcagca gcaaaagtat tttatgatcc aaggaatttc aaacgaaaag
    25561 gtgctatgcc aaaattagta ttgaagacac tttttggtca aggtggagta cagactttgg
    25621 atggagctgc tcatcaccat cggaaaaata tttttatgga tctaatgaca ccggaaagaa
    25681 tggaagatta tcatcgtatc ttagataaga atctgacaca agcacttgaa gcacagcatg
    25741 gtcaatttga actatttgat ttaagtaaga tggttttctt tacatctatt tgcgagtggg
    25801 caggtatcaa tttatcagcg atttctaaag atgaggtcga aaaattagca gaatatcaaa
    25861 tctctatgat cagcggaacg tttacttctc cgattgatca cataaaaggt gtcgaaaatc
    25921 gcaaaaaatc agaaaaatgg gcgcaaggct tgattgaaga agctcgacaa aatcctgtgg
    25981 ctggaaaaga aaatgtggca ttatatgctt ttgccaatgc cacagattta gatggtcagc
    26041 ttttacctct ggaagtggca gctgttgaat tattgaacat catccgtcca acggttgctc
    26101 taactgtgtg ggcggcattg atgggacatg cacttttcag tagaccagat ttgtatcagc
    26161 aattaaaaaa tgacttttcg acgttacaag atccgtttat tcaagaaatg agacgctatt
    26221 atcctttctt cccaatgcta cctgcgattt cgctgaaaga agtagaagta gatggttatc
    26281 gtataccaga aggcagttgg gttattttag atttatatgg aacagaccat gatgaacgaa
    26341 cagttgaagc gccagattcc tttatgatca aacggtatgt agggaaagca aaagatattt
    26401 cctataaaga agaatatgag atgattgcac aaggcggggg aaattttcga caaatgcatc
    26461 gctgtgcagg agaatggatc actcttcata gtctacgtgt gttttccgat cagctagtaa
    26521 ataagtttga gttctctgta cctgaacaag attggacgat tccattcaat cagtttccga
    26581 cttatccgaa tagccgagcg ctactttaca agaattagat tagcataaac ttatcgaaaa
    26641 aaaggaagat caaacagtgc tgatcttcct tttttcatat tactataaat tgataaggtc
    26701 aattgatcaa aatccacgaa atgcttcatc aatggtttga taatcttcat tggataaatg
    26761 caaatctaaa gcttggacat tgtcagatac ttgctctgga cgcttagctc caggtataac
    26821 tacagacact cgtggattct tcatgtacca agctaaaaca agttgcgcag gagtggcttg
    26881 gtatctttta gcaatggggc gaagtacatc gacagcggtt aaaatttctc tgaaacgttc
    26941 tccttggaaa tctggatttc tttttcgagg atctccttca ccaaattgtt tttcctcacc
    27001 aagttcatat tttcctgtca gcaaaccgga agcaagagga aaatagggaa caaaactgat
    27061 tttgtttttt tcaagataag ggaataattc tttttcagcc tgccgatgga tcaaactgta
    27121 tttatcttct actacatcga catatccatc tgcattcgct tctttcaatt gttctaacgt
    27181 gaagtttgac acaccgactg ctcggatttt tcctgcttct tttaattcgt gcaatgtagc
    27241 aactgattca ttttttggtg tgctttcatc cggaaagtgg atataaaaaa tgtcgatgta
    27301 atcagtttgc agacgtttta gtgcatcttc caccgattgt ttgagaaact caggagaatt
    27361 atcaaatgtt cgccctttat ttggtacatg agcagctttt gtagcgatga cgacacgtga
    27421 gcgatcatat tctttttcct tcagtacctc gccaatcagt tcttcggaac ggccgtttcc
    27481 gtaagcataa gcggtatcga tcaaattgat cccgctattc aaagctgtac gaactacttg
    27541 ttttcctgtc tcatcttcca aaccgtcaaa aagattatgt cctccaacgg catttgctcc
    27601 taatccaagc tgttctgcgt agacttgtga atgtccgatt cttatttctg ccatagctga
    27661 ttcctccttt ttgtctagtc taaaacaaaa taccaaaaag ctgaactaaa acgtttttgt
    27721 gaggggaaat agtcagatat ctcttgactt tgagactggt ttttagtagg ttataagtaa
    27781 tatcgaatgt caatgaaaga gcgagtaagt ttttgaaact ttacaagaga ggatcatcga
    27841 ggctgaaaat gatctaaagt ttcattagcc taaagacact ctggagatgg acgagtgaaa
    27901 agccgtcccg cgtacgagcg ttattcgtaa gaggaaagag aaatctttca aaattaggtg
    27961 gtaccgtgta tgaatttcat tcacccttgt tcatgatgaa caagggtgat ttttttattt
    28021 acatagctaa gaaagggaga gaaaactatg agtttccaaa gaccaaaagg aacaaatgat
    28081 attttgccag gcgaatcaga aaaatggcag tttgtcgaag aaacagcacg tttattattc
    28141 agagattatc agtataatga aatgcgtaca ccgatttttg aacactatga agtgatatcc
    28201 cgaagtgtag gggatacaac ggatatcgtt tcaaaagaga tgtatgattt ttatgacaaa
    28261 ggggaacgcc atgtgacgct tcgaccagaa ggtacagcac caatcgttcg ttcttttgtt
    28321 gagcataaat tgttcggccc agagtatgcg aaaccgttca aaacttttta tatgggacca
    28381 atgtttcgct acgaacgccc tcaaaaagga cggttacgtc aattccatca aatcggagta
    28441 gaagcatttg gatcagaaaa ccctgcaact gatgtggaga gcatggtgat ggcacttgat
    28501 ttcttcaaac agttggggat cacgcaaatc cggctagtca tcaattcatt aggtgataaa
    28561 gaaacgcgtc aagcctatcg tcaagcattg atcgactatc ttttaccttt tgaatcagag
    28621 ctgagtgaag actctaaacg ccgccttcat gaaaatccat tacgcgtatt ggacagtaaa
    28681 gacaaacgag atcaaaaatt tgtggcagaa gctccatcta tcttagatta tctaagcgag
    28741 cctgctcaaa aacattttga taccgttgtt tccatgctgg atgcattggc tgttccttat
    28801 gaaatcgaca gtaatatggt tcgcggacta gattattaca cacatacgat ttttgaaatc
    28861 atgagtgacg caccaaaaat gggggcacaa gcaacgattt gcgcaggcgg acgttatgac
    28921 aatctagtgg aagaactagg tggtccttca acacctggct tcggatttgc aatgggtatc
    28981 gagcgattac tcttgactat ggaggcagag gaagtagtta tccctgcatt taatgagttg
    29041 gatgcttacg tggtcgcact cggagacgaa acaaatatcg aagcattgaa agtggtccaa
    29101 gcgattcgaa actttggttt ttcagctgac cgcgacttca tgaaccgtaa agcaaaagcg
    29161 caatttaaga cagcagataa agcgaatgcc aaattagtat tggtgatcgg tgaagatgag
    29221 cttgctagtc aaacgattaa tgtcaaagca atggcaagcc gaaaagaaaa aagttttcca
    29281 ttagaagaga tatatgaaca ttttgatgaa gtctatgatg aaatgacttt attcgagtag
    29341 gaggaaaaaa ataaatggca caaagaacga tctattgcgg attagtttcc gaacaacaat
    29401 tagaacaaac agttacatta aaaggatggg tacaaaaaag acgagacctt gggggagtaa
    29461 tctttatcga cctacgtgat cgtgaaggaa tcgttcaaat cgtctttaat cctgaaatca
    29521 acaaagaagc atgggaaatc gcggataaat gtcgcagtga atacgtgatc gaagtgactg
    29581 gggaagttag aagacgcgga gaaaatgcag taaatcctaa aatggccact ggtgaattcg
    29641 aagtgattgc aagtgaaatc gtgattttga atcaagcaaa aaccccgcca tttcaaattg
    29701 aagatgatca agcaatcagt gacgaaatac gtttgaaata tcgttatttg gatctgcgtc
    29761 gtccaaaaat gactaaaaac atcaaattaa gaaatgcgac aacaaaagcg attcgtcatt
    29821 atttagatga taatgacttt ctcgatatcg aaacacctta cctagggaaa tctacaccag
    29881 aaggcgcaag agactaccta gttccttcac gtgtccatgc tggacatttc tatgcgttgc
    29941 cgcaatctcc tcaattgttt aaacaattgc tgatgaatgc tggattcgac cgttattatc
    30001 aaatcgttcg ttgtttccgc gatgaagatt tacgtggaga ccgtcagcct gaatttacgc
    30061 aagtagatat tgaaacaacg ttcttaacag cagaagaaat ccaaacttac acagagggat
    30121 tgattgcgaa ggtaatgaaa gaagttcgcg gtatcgaagt cacattgcca tttcctcgta
    30181 tgacctatga tgaagcaatg gctcgctacg gcagcgacaa accggataca cgttttgata
    30241 tggaattgat cgatctaagc gatacagtca aagaagtaga attcaaagtc ttccaaatgg
    30301 ctcttgaaaa cggcggtgtc gtgaaagcat tgaatgctaa aggcgctgca gatcgctact
    30361 cacgaaaaga catggatcag ttaggtcaat atgtaggaca attcggagct aaggggcttg
    30421 catggctgaa agtagaagaa gacggattga aaggtccaat tgctaaattc atgggagaag
    30481 caacagaagc aatcattaaa gcaacagatg caaaaccagg tgacttgctg atgtttggcg
    30541 cagataaaag tgaaatcgtc gctgctgcat taggtgctat ccggacacgt ttaggaaaag
    30601 aattaggact aattgatgaa tcgaaattca atttcttatg ggtaactgat tggccacaat
    30661 ttgaattttc agaagaagaa ggtcgatatg tctctgctca ccatccattc acgatgccaa
    30721 aagaagaaga tatcccgtta ttggctgaag accctgcaaa agtctatgca gaagcttatg
    30781 atatcgtctt gaacggttat gaattaggcg gaggttctat ccgtattcat actcgtgagc
    30841 ttcaagaaaa agtactagaa actctaggct ttacaaaaga acaaatggaa gaacaatttg
    30901 gtttcttgtt aacagcttta gattacggat tccctccaca tggcggtatt gcattaggat
    30961 tggaccgttt tgcaatgtta ttagccggag aagataatat tcgtgaagtc attgcctttc
    31021 ctaaaaacgg aaaagcagca gatgtgatga gtgatgcacc aagtatggtt tcgcctttac
    31081 aattgttcga attgaatatt gatgtaacga atatagaaga atagccgata aaaaggtaaa
    31141 aaggatttgt cgaatacgct tcttttgaca accttgattt aattgaaaaa cactttataa
    31201 tgaagccata acatataaag cggggctttt ccatgaaaaa aacattcgaa aattgggctt
    31261 atcatgatta cacgaccaag ctttcggtat ctatcgttta tgcgttgatc gcatcagtcg
    31321 cattgaattt cttttatcag ccaggacata tttattcaag tggtattact ggtctagctc
    31381 agattttgac gactttgtca aaatcactga taggattcga agtaccggta tcaacaactt
    31441 tgtatttact gaatatacct ttgtttgtat tggcgtggtt aaaaatcggg aagaagttta
    31501 caattttcac ttttatcaca gtcactttga cctccctttg tatccatatt ttaccggaaa
    31561 cagtcctatc taccgatcca atcatctgtg cgttgtttgg gggggctgtg atgggactag
    31621 gtattgggct agcgttgaga aacggcctat catcgggcgg tctagatatc gtgagtatca
    31681 cgattcgtaa aaaaacagga aagtcgattg gttctatttc gatctacttc aatgcattga
    31741 tcgtttttgt tgccggaatt ctatttggct ggcaatatat gttttacagt acgttgtcta
    31801 ttttcgtcag tggaaaagta actgatgtcg tttatactaa acaaaagaag atgcaggtaa
    31861 tgatcgtaac gaaacatcca gaaaaagtag taaatgaaat ccaaaactgt atgcgtagag
    31921 ggatcacgat catcaatgaa gcagaaggtg cttataagca tgataagcag acagtattga
    31981 tgacgatcgt tacacgatac gaactgcctc tacttagaga agcaatgaaa gcttctgatc
    32041 cacaagcttt tgtaagtatt gcagaaaacg tccagatact tggtcgtttc tatgaggatg
    32101 gcatttagat cgagtttgac aaaaattcat gaaatactgc tgtagacaga cgttcgtttg
    32161 tctgcagttt ttttctacca tagtagaatc agcaaagaaa aactgctttt ttatttgaaa
    32221 ttttcttaaa gttgaaaaaa agcatgttta tttttttaaa tttatgggat gatagaaaaa
    32281 aatagtagag aaaggacacg attctacttg ctattttcac ttaatacccg tatattacga
    32341 atgaaaaaag tttatgaaaa gttggaatat tgaatggaaa aacaaaaaag gttaaaaaac
    32401 agccgctaca tcactgggtt tgatgggctc cgttcattag ctgtggtggg tgttatcttc
    32461 tatcaccttc tgccaactag tatgaaaggc ggctatttgg gcgttccgat ttttttcgtc
    32521 gtatctggtt atttgatcac ggatctgctg cggcaggaat gggaacaaaa tggaaaaatc
    32581 aatatttggc aattttatgt cagaagaatg aagagactct atccggcact tgtttttatg
    32641 cttgtgacag cttcggctta tatcactttg tttcagcgag ggctgttaaa caatcttcga
    32701 ggagttgtgg tcagcagctt actgtatgtc aacaactggt ggcaaatcaa taacggttta
    32761 tcttatttcg atcgatttgc taatgagtca ccgtttacgc atatctggtc tttagcagta
    32821 gaaggtcaaa actatttggt ttggccgatc ttgtttgtat tgttgatggt atttgttcgt
    32881 aaaagaaaat ggatctttta tactgtgtta ggtacatcct tcctctccgc catcttgatg
    32941 atgatcttgt atacaccagg aggcgatcca acccgtgttt attacggtac cgacactcgt
    33001 ctgttttcta tatggttggg aagtgcgctt gcgtttgttt ggccaagtac gcgtttaaag
    33061 aaaaacatcc cgaaacaagc gaaaaatatt ctgaatatcg ctggaggaat ctcgcttttg
    33121 gcactgattc tttgcttctt ctttttagat gatcattaca atttcatcta ttacggagga
    33181 atgctcctag tgagtctgtt atgcgtagtg ctagttgcag tgacagcaca ccccggagca
    33241 agtttgaatc gatggctgac aaatccagta tttacttgga ttggcaaacg aagctatggc
    33301 atttatctgt atcagtttcc agttatgatt ttttatgaag caaaggtgac tgatattgcc
    33361 gatcatgttt tgttgcatac tttagtggaa atcacattga ttctaatcat cagtgaactt
    33421 tcttatcgat atgtggagcg tccgctagca cgttttgatt atggtcgtac attccaagtg
    33481 atgaaaggat ggttcagcaa accgatcatc agtaagagca aaccatgggt tgttccaggc
    33541 acattacttg tagcaattgc actcgttggt ttcgttacgg caccgaaaaa cgctgtaaca
    33601 gccgatcaaa aacaactgca ggcaaaaatc gctgaaagta aaaaattagc agaagaaacc
    33661 agaaacaatt cagaaaacac cactagttcg gttgaccaag cgatccttga taagtacgag
    33721 ttgtccaagg cacaagggaa taaggcacaa aaattagaac taacggcatt tggtgattca
    33781 gttatgttgg atgctgcaac ggatctacaa gaactttatc caaaagtagt ggtagatgga
    33841 gatgtcggac gacaattata tgccagtgta ccgtacattg aaaaattaaa ggaacaagat
    33901 ttgttgaaag acactgttct gattggacta ggaacgaatg gctcattctc ggaagctcag
    33961 tttgatagtg tgatggatgc aatcggtgat cgaaaagtat actgggtcaa tgtccgagta
    34021 ccgacgaagc gctggcaaaa tgacgtgaat gcgatgcttg aacagatgac gaaaaaatac
    34081 gacaatatga cgctgattga ttggtacagc tacagtaatg accatagtga ctggttttat
    34141 gatgatcaag tccatccaaa tcctgaaggg atgacacatt acattcactt agtcagcgaa
    34201 gccttgctag gaggaagttc aagtacgaac agcagtgaga caaacgtgac agaatgatag
    34261 ctacaaaaaa cttcaggcaa aaacatttgt ttttgcctga agttttttta tatttaccgt
    34321 gtttttaaca ttcatataga ttcaggaagc aaaacggttg agtgtgtctg taaataacgt
    34381 tctttttgtt ttcgggtgag ttttttgaat gcagcttctg cttttgttgc ttcgcttctt
    34441 gaagaaaata cttctgcatg gatcatctta acaggtcttc ttttttgaag acgagtatat
    34501 ttagcaccag taccattgtt atgttcctct aatcgtctag aaagatcggt tgtatatcca
    34561 ccataaaatg tctgatcttt acagaataat acgtaaaaat aatgttcatt tgccatagag
    34621 catctcacga atctctgcag tgtattctcc atccatattg taagtgatca atggtggagc
    34681 gactttgaat ccgtctaatt tgccttcttt gattccttca actaacaaaa tatttgcctc
    34741 ttttccaggt tttgggtaaa caaattggat ccttttaggt gcaattcgat agtcttccat
    34801 gacctgaatg atttctaaga accgatcagg acgatggacc atagtaaagc gtccatttgt
    34861 ttttagtagc ttacttgaaa tatcgatcac ttcttctaac gtcgtatgaa tctcatgacg
    34921 ggcgattgcc aggtgtttat tagggttcgt tttatttgtt ggcagccctt tgaaataagg
    34981 tggattacag acgactaagt cacaagaatt atgtcgaacg acagataagc tgtctttcag
    35041 atcgatggtg tggacagtca tttgttcatt taaatggtta agcgcgatac tacgtcttgc
    35101 catgtctgct agacgaggtt gaagttcgat agcgtcgata gccgcctggg ttcgtttact
    35161 taaaaaaagt ccaactgcgc catttcctgc acaaaggtca acaatcttcc cttttttagg
    35221 tacacgggaa aagtgagcaa gcagcacagc atctaaagaa aaagaaaaca ctttggaact
    35281 ttggataatc ttgatatctt ctgcgtataa ttggtcgatt cgttcgtctt cgtgaagcat
    35341 ggattccctc caaaatgaaa aaaatagtcg tctgtattat actattttta ttaaaaagaa
    35401 ccaaggtggc ttgtcaaacc tctatttttt atgcatacta tgaccataag aagggaaagg
    35461 aagagactat gttttatcga tttatgcgcg gggtagttcg agtactatta gccttagtta
    35521 atggaaatgc ccgctatgag aataaagaag ttttaccgca agatgagaat tacatactag
    35581 ttgctcccca tcgtacttgg tgggacccat tatatctggc ggtagctgca gcacccaagg
    35641 aattcagctt tatggcaaaa gaagaactgt tcaaaaatcc agttctgcga tttatattaa
    35701 ctcgatccaa tgcttttcca gttaaaagag aaaatccagg accaagcgtc attaaaacac
    35761 ctgtaaagat tttgaaatcg acaaatttga gtttgatcat gtttcccagt gggacaagac
    35821 atgctacaga acttaaaggc ggaatggcat tgatttccag aatgtctaaa gcgaaaatcg
    35881 tgccagctgt atatcaaggt ccgttgacac ttggggatct gttcaagcgt cagagagtga
    35941 cagtgcgttt cggcgaacca atcgacttat cagatatcaa gaaaacggat aaagaaggta
    36001 tggcggagat cgaacgacga acacaagcag cttttgacaa attggacaaa gaagtgaacc
    36061 ctgatttcaa atatgaagtc aaaaaaaaat aaaatgataa gtttatgcgt gggcggtctt
    36121 ttgaatctac tgtcctgttc agttcagact ttttcaatga aaacgaggga gagacgtcaa
    36181 gtctgccctc tttttgcgtt ccaatatgta aaatagaaga caggtgtcat gtaatggatc
    36241 aaagtgttat aataattaga aggacatttt ttgaggtgat tgaatgcgtt ttttacatac
    36301 agccgattgg catatcggaa aaaaactaca aggttatgac ttgattgaag accagcgtca
    36361 tgtactatct tctatactag atatcgcgaa agaagaaaaa gtagaggcaa tcgtgattgc
    36421 cggagactta tatgatagga gtgtcccagc tgttgatgcc gtagaattat acaatgaatt
    36481 gatggtcgat tggaatttaa aggaaaagat cccgattttt gctatatcag gaaatcacga
    36541 tagcagtacg agactttcag cgggtacgcc atggttcagt tattcgaact attttttgcg
    36601 aacacagcta gcagaagcat ttgaaccagt cgagtttaaa aacgtacaat ttttctttct
    36661 tccttacttt gaaccaattg cagcgagatt atattttgaa gatgacaata tccgaacgat
    36721 caagcaagcg gtagccaagg tgatagataa aatgcaggag ctatttgatt cagaaaaaaa
    36781 acaagtattg attagccatt tttttgtaat gggaagtgta aaatctgatt cggaaacgaa
    36841 aatcgaagta ggcgggttgg attcgatccc tggtgaacta cttacaccgt ttgattatgt
    36901 agcactagga catttgcata atcaagctgc tttgagacag gataatgctc gctatagcgg
    36961 ttccccattg aaattctctt tatctgagat caaccaaaaa aaaggcgtat ggatcgttga
    37021 cctgacaaca ggcctggagt tgaattttag ggagatcgaa cccctgcatg atattgaaga
    37081 aataacagcg agtttcaaag aattgctgga tcctgccttt tatcagtccg tcaatcggga
    37141 gaattacatc tatatctatc tcactgatag aacgatcatc ccgaatatga tgaaccagct
    37201 tcggcaaata tatcctcgta ttttggggat tgaacgggca aacggtcgag cagaacaaac
    37261 aaagcgcaaa cgaaaagtac tagaaatgaa agatcctcaa aaattagcag aagcattttt
    37321 tagtgaaatg acggaagagc agctaactgc aaaccaagaa aaatggctcg gagaaacgtt
    37381 gcaagaaatc atggaaatgg aatgatcaag aatgaaaccg aaaaaattac aacttaaaaa
    37441 ttttggtcct tttatcaatg agaccgtaga tttttcacgt ttgacagaag caccgctttt
    37501 tttgatcagc gggaagacag gagctggcaa aacgacgatt tttgatggga tcaccttcgc
    37561 actttttggt gaaacttccg gaagacttcg ctctggaaaa gagatgcgtt ctttgtttgc
    37621 tacaccagac gaagaaacaa gcgtgacttt ttcttttgaa catcagcaga tgagctatga
    37681 aatcgaacga aaaccagaac aagttttagc aaaacgaaaa ggaaacggaa caaaaaaaca
    37741 ggcagccaaa gtaacgttga ccatttttga tgggaaagga aaagaaataa aacaaatcac
    37801 aaaacgtaca gaagcagatc agttgatcag agaactgatg aatctcgatg cgaaacagtt
    37861 ttctcaaatc gttttactac cccaaggaga attccgtaat tttttggttt catcaagcag
    37921 tgataaagag actgttttac gtcatttatt cggtactcaa tttttccaac agttcaatga
    37981 gcgattaaaa gaaaaagcaa aacaacagca acaaaaattg gatcatttag agcaagagct
    38041 gcatcttctg caaaaaaggt ttgttttagt caaagaagag gaaccagcag cagggtttga
    38101 agccgtttta gcccaatgga caaaccatca ggcaatactc gaaaaacaaa tcacggaaga
    38161 aaaagaagct ctaacggaac ttcaggaaaa acaaaagcaa ctagaaaatt cctactatcg
    38221 ttttgaaagc ttgaaaaaaa gttatgaaga aaaaacgata ttaataaaca agcaaaagga
    38281 attagctgct caaaaagacg aaatagagga aaagaaacga tggattgggt attatgaatt
    38341 tacgcaacga ttagtcgaac caatccagcg cctacaagaa ataaaagttg aaaaaataga
    38401 actgtcacaa aaaagtagtg agcaaacaaa gaaactagcg atgactacgc agaattatga
    38461 agagtggcaa aaaaatgaag caaaccgcgt acgccgacaa acaaaaatca aagaagctgc
    38521 tcaaaaactg caaaacaaac agatgatggt gccaattgtc gaatccttac gtagtaaaaa
    38581 acaagacgca gaacaattaa caatttcatt ggataaagaa gaattgaatg ccgagaagct
    38641 gtatcaaaat ctcgctatct atcaggaaag aactcgaatt cttcaagaaa atttactgga
    38701 tcaggaaaaa ctgcaagaag atcgtcttgc ttttggacag ttaaaatatc tagatgaaaa
    38761 gcagcaagaa atagaaaaac aaaaagcaaa agacactggg tcgctcgcac agataagaga
    38821 agaactcaaa aaagcaactg aagaaataac aaaaaatagt cagctattga aagaacagac
    38881 agaacaagca gaacagctga aaagccaatg ggcaaagcag caaattgctc gtcttcagct
    38941 ttttttgcgt ccaggggagc catgtcttgt ttgtggatca acggaacacc ccatacaaca
    39001 attaaaacac gaacagccaa ataaggaaga tatcttaaaa gtagaacaac aactggcaga
    39061 agctgagtct gctgtagaaa gaactcagca aatcattgcc aaaagtgaag cacatgaaaa
    39121 taatcttcaa caacaattat cagatttaga acaaaaaatc atggcaacga atatacaatt
    39181 ggctgaacaa gaagaacact tgcaacaggc tttgaaaaag gcatttcctg agataacaga
    39241 acaaaaaaca aaaacaactc ttgtagtaat cgaacaatct ttagatagac gaaaaagcaa
    39301 gatggccaat gcaagaatag agctggaaga aataagacaa caagcagaaa agctcgaaca
    39361 acaattagct gtctacaaac agcagcagac agaaaaaaat aatcagctcc agcaactgca
    39421 aggagaaatc aaaagtttag aacaacaatt gcaagatacg gatagtaact gtttagaaga
    39481 ggagatacga cgacttgaaa aacagttaga agaagactcg aatcaactag ctgttgagca
    39541 gaaagcagga gtgcaattgt tgcaagatct tcatacgctt cggacacagc ttgatcttca
    39601 acatgaacaa atagaaaaaa tagatgaaaa aatagaaaag gaacaagaaa gaatagctaa
    39661 aaagttagaa agccagtcgt atttttcaac aattgaagaa atctgtgcat ttgcacaaca
    39721 gcaagcacaa tataaagaaa accaacgtac agttacttct tatcatgaag agcaattagt
    39781 gacagctgac cggctgcgtc aattagaaaa tatccagcta actgagacat tcccagatac
    39841 agaagagatc agccaacagt tagaagaaat aagacgtatg atcgaaataa agcaagaaaa
    39901 gatatacggt ttacaagaac aaaaaattag taatcaaaaa atctacgatg aatgtctttc
    39961 gatttatcac acaagccaga cgaaattaga tgagcttacg caattacagc agctttcgca
    40021 aacattcaac ggagaaaatc ctaagaagac tagtttggaa cggtatgtgc ttcaggttta
    40081 tcttcaagaa gtgttacaag tagcaaatga tcatctccaa cgtttaacga aaaatcgtta
    40141 tcaatttgag ctagcggata ccattggcag ttatcgaggg aaaaccggat tagaaatcaa
    40201 tatctatgat gatcaggcag gtatgtctcg aagtgcgcat accctgtctg gcggtgagag
    40261 ttttattgca gcactttctt tagcattagc tcttgccgat gtgatccaga cacaagctgg
    40321 tggtgtagca atcgatgcat tgtttattga tgaaggattt gggtcattag atgaagaagc
    40381 tttggaaatg gcaatggaag ctttagaaac gatcgaaagt gaaggaagaa tgatcggcat
    40441 cattagtcat gtaagagaat tgaaagaacg agtattacaa caaataagaa ttgatacaga
    40501 aggtagcgga caaagcaaaa tacgctatca acttggataa aaagggagat tggtaactat
    40561 gaaatggagt attccagaaa aagtcattgc acgtggacgt caatacttgc aagaaggccg
    40621 agtgttatcg gttgtccctg accaagaaga aaacgtgtgg catgcagaag tcttgggcag
    40681 tgaattgtat atggttgatt tggatggtac agcaaaagag atagattact gccaatgtcc
    40741 ctattgggag gaacataaat attgtaagca tacagttgct gtggaacttt atttacggtc
    40801 gaaaaatcta tctcgtatca tgaaaaaaga ccagccaatg ctagaaccgg taaaagcaac
    40861 aagtgaggga gaaatcattg caaaaggttt ttctcgtcta aaaacacaaa acaatcctca
    40921 agcggtgcaa cctttgatta ttgaatacca agtagaaacg attgagacta atcagtatca
    40981 cccagagctt tctattttgg cactttactt gcgtatcggt tcattggcat caccgaaaaa
    41041 aacatatatc gtaaaaaaca tctatgattt tttacaagct tacacagaaa aagaaactta
    41101 cactgttaat aaacagtacc agtttcgtct agataaacgg gcgtttcaaa aagaagatca
    41161 aaaaatactc acgcatcttg caggaagtgc tcagacgcat caattattag gtacgaacgg
    41221 cttacaagtc aaaggaaaac tagataaaag atatctgctt ctcccagtag aacaagcccg
    41281 cttcctgtta gagcagatga atcagactac acgtttgcat ctgcagataa atgaacaaaa
    41341 cgatcaagga atacaatttt ccgatcaaaa caaaccttta tcattcaaag tggaaaaatt
    41401 tggagaagat tatttgctgc aagcattgaa tgattttgat tttttcttta tgcattatca
    41461 gtggggagtt atccaaggga cattttattc tctgacgaaa tatcaacaaa cggtttatca
    41521 aacttggaaa caattgattc gccgattgga gaaaccagaa gtagtgttca agaaaaaaga
    41581 attaccgtct ttgtttaaag agattatccc tctgctgtca gagatcggcg acgtagctat
    41641 tgcccctgca gtagaagagg aagtagcaga tatccccgtc gaatttattt tctttttccg
    41701 aaaagcaaaa ggaaaaatcc aagctcgagt cgatttcgtc tatggtgaag tcatctattc
    41761 gactgacccg aagcatgagg tctcgacaga acaggctttt caggtgttac gtgacacagt
    41821 agaagagaaa cgagtacttg atctattcag aatgtatcgc taccagaaaa atgaaactgg
    41881 ttatgaacgt ccattgccaa gcggggaaga actatatgcc tttttccgca cagaactatc
    41941 tgtcttccgt cagtttggtg aagtcagact tggaagaaaa ttgcgagagt tgtatttaga
    42001 tgcgcataaa catcaaccga aaatcgaagt aaccgagacc ggttcatggc tcgatattcg
    42061 attcgatgtg acagggatag aggagcaaga aatcgatcat gtcctgcaaa gcttgctgag
    42121 aaatgatgct ttttatacat tagaaaatgg tcaggttctt tcttttgatt cagaagaatt
    42181 tcaacaaaca agccaagtgt tgcaacaatt acgggaatcg atacgtacag aagaaggaac
    42241 gatccatgta cctaaaaatc aaggattgat cattcaaaat caattggaaa aaagcaatgc
    42301 tacttttagt gaatcgttcc aaacgatggt acaagatctc attcatccag aaagatacca
    42361 ggcacagctt cccaaaggat tgaatgcaac gatgagagat tatcaaaagc aaggttttcg
    42421 atggttgaag atgttaggtc attatcagtt tggcggtatt ttagccgatg aaatgggact
    42481 tgggaaaaca ctacagacaa ttgccttttt actttcggaa aaagaagaac ggaaaagctt
    42541 ctctgctttg atcgtagcgc ctgcgagctt aatttacaac tggcaggcag aggtgagaaa
    42601 attcgcccct tctttaagta tccaagtaat caatgggaat aaaaaagaac gtgaagaatt
    42661 actggctaaa gacacagata tccgagtgac ttcttatgcc agcttaagac aggatctagc
    42721 taattatcag tcgcaaaaaa ttgattattt gattcttgat gaggcacaaa tggtcaaaaa
    42781 tagcagtacg aaaactgcac aagcattgag agaattagct gtacctcagc ggtttgcact
    42841 gagtgggaca ccaatcgaga ataatttaga agaattgtgg tcattgtttg caacgattat
    42901 gccaggattt ttcccgacta aaacaaaatt cagggaactt gccgcagaag aaattgcgca
    42961 aatgattcga ccgtttattt tacggcgaga taaacaaact gtgttgaaag acttgccgga
    43021 aaaaacagag atgaatctat attctgcact tacagaagaa caaaaaactg tttatttagc
    43081 ttacttgcgt caaatgcgtg aagaaatcac atcaatggat tctgaagcat tcaagaaaaa
    43141 ccggatcggg atacttgctg gtctgacaag actgcgacaa atttgctgcg atcctcgtct
    43201 gtttattgaa gattaccaag gcggttccgg caagctcgaa caagtaaaag atctactggt
    43261 agctgccaaa gagaacaaac gaagagtttt gctgttttct caatttacag ggatgctgac
    43321 tatcttgcaa gaagagttag ccgagctagg gatctcgaca ttttatttaa gaggcagtac
    43381 gaaaccacaa gatcggttaa gtatggtaga cgcatttaat gctggcgaaa aagatgtttt
    43441 tctgatttcc ttaaaggctg ggggaactgg attgaatctc acgggagcag atacggtcat
    43501 cttgtatgac ctttggtgga atcctgccat tgaagaacaa gctgctggac gtgcacatcg
    43561 gatcggacaa aaaaatgtgg tagaagtgtg gcgtatgatc tctgaaggaa cgattgagga
    43621 acgcatggat tcactccagc aagaaaaacg cgaactgttc caaaaagtga tccaaggtaa
    43681 tgaagaacag ttgacgaaac tgactgaaga agacatccgc atgattttaa gtgtaggaga
    43741 aatcgaggag taagagactc gaaaaagcgt tcagtaatgg gtaagaaagc ctttctaaag
    43801 aagcgattgt ttatttgatt taaaaaatag caaggaggct gggattccat tttatcccag
    43861 cttccttgct atcatttttt tatttctatt tttaaaggtt atacaaagat tgtcttcatt
    43921 gtctctaaca gctgatcaaa agaagaaatc tcgatatcgc aaattggagt ctgttcaatt
    43981 ttgcgttctc gatggttgaa ccaaagcgct tgccagttag cactttttgc acctagaaca
    44041 tcattgtcgt aattatcgcc aacgtataag gtctcttctg gaagcatatg aaattctttc
    44101 tcagccagct gaaaaatctc tttttctggt ttttggaaac ctgttgcttg agaaatgagc
    44161 atattgcggg aaggaatcca attgttcaat tgaagttgct tcagtttttt tgtctgatgg
    44221 tcagtcggcc catttgtgat aattcctaat gggacattct tttccttcaa gaaatcgagg
    44281 gtttttttga cctcctcatg caagcagata ttatcaagtt cttcctcgta aattttttga
    44341 aataagaggc catcttcttc cgtaatatga gggtagtcta aatcttttag tgattggctg
    44401 atccgatgtg ctcgcatata ttctagtgtc caatctcctg ccattacttt tgggaagttt
    44461 tcatcactat gatggcggaa ccggatatat agtggatgca tatctgcatc tgaaacaagc
    44521 ggaacgactc ttttgaccgc gtttcgaaat ggctgctgct gatcatacat cgtatcatca
    44581 acatcaaaca caactgattt aatcaaaatt ttacacgcct ttcggttttc attgattttt
    44641 tcacaaatcc ttgactattg taacagaaaa aattgtctaa aaaagctgat aaataaaggt
    44701 ttttcttact atatttctat ttccttgaca gaataaggtt tttatatttc agtttttttc
    44761 actaaaaatc gtgaaacacg tcaataagtt tgatgaacaa attaatcatt atggtagaag
    44821 tcgattgatt tattttaatg caatctctgt ggaattttcg aaagatttat tttt
//
LOCUS       DAZKMB010000009        44838 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000009 DAZKMB010000000
VERSION     DAZKMB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.009"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8V3_000471"
     CDS             212..604
                     /locus_tag="ACG8V3_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0299038.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8V3_000472"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8V3_000472"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0299039.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8V3_000473"
     CDS             2698..2943
                     /locus_tag="ACG8V3_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299040.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8V3_000474"
     CDS             3165..4775
                     /locus_tag="ACG8V3_000474"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0299041.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8V3_000475"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8V3_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299042.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8V3_000476"
     CDS             5067..5936
                     /locus_tag="ACG8V3_000476"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0299043.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8V3_000477"
     CDS             6101..7372
                     /locus_tag="ACG8V3_000477"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0299044.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8V3_000478"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8V3_000478"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0299045.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8V3_000479"
     CDS             8763..9026
                     /locus_tag="ACG8V3_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0299046.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8V3_000480"
     CDS             9471..9920
                     /locus_tag="ACG8V3_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0299047.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8V3_000481"
     CDS             9977..11395
                     /locus_tag="ACG8V3_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0299048.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8V3_000482"
     CDS             11395..12255
                     /locus_tag="ACG8V3_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0299049.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8V3_000483"
     CDS             12429..13202
                     /locus_tag="ACG8V3_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0299050.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8V3_000484"
     CDS             13349..13714
                     /locus_tag="ACG8V3_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0299051.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8V3_000485"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8V3_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0299052.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8V3_000486"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8V3_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299053.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8V3_000487"
     CDS             15560..16216
                     /locus_tag="ACG8V3_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0299054.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8V3_000488"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8V3_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0299055.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8V3_000489"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8V3_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0299056.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8V3_000490"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8V3_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0299057.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8V3_000491"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8V3_000491"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0299058.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8V3_000492"
     CDS             21888..22202
                     /locus_tag="ACG8V3_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0299059.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8V3_000493"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8V3_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0299060.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8V3_000494"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8V3_000494"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0299061.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8V3_000495"
     CDS             23546..23875
                     /locus_tag="ACG8V3_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0299062.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8V3_000496"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8V3_000496"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0299063.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8V3_000497"
     CDS             24863..25687
                     /locus_tag="ACG8V3_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0299064.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8V3_000498"
     CDS             25680..26027
                     /locus_tag="ACG8V3_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0299065.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8V3_000499"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8V3_000499"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0299066.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8V3_000500"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8V3_000500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0299067.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8V3_000501"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8V3_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0299068.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8V3_000502"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8V3_000502"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0299069.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8V3_000503"
     CDS             31684..32451
                     /locus_tag="ACG8V3_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299070.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG8V3_000504"
     CDS             32441..34483
                     /locus_tag="ACG8V3_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0299071.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACG8V3_000505"
     CDS             34764..34913
                     /locus_tag="ACG8V3_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0299072.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8V3_000506"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8V3_000506"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0299073.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8V3_000507"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8V3_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0299074.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8V3_000508"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8V3_000508"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0299075.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8V3_000509"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8V3_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0299076.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACG8V3_000510"
     CDS             39330..40412
                     /locus_tag="ACG8V3_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0299077.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACG8V3_000511"
     CDS             40412..41215
                     /locus_tag="ACG8V3_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0299078.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACG8V3_000512"
     CDS             41285..42025
                     /locus_tag="ACG8V3_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0299079.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACG8V3_000513"
     CDS             42036..43040
                     /locus_tag="ACG8V3_000513"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0299080.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..>44838)
                     /locus_tag="ACG8V3_000514"
     CDS             complement(43085..>44838)
                     /locus_tag="ACG8V3_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299081.1"
                     /translation="TGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSKYTR
                     TIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGL
                     SLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMI
                     IAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHS
                     FLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELM
                     PIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTT
                     INWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEV
                     NTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARY
                     QKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSI
                     HEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDT
                     FIDRITVQLSRNYPNPV"
BASE COUNT        14407 a         7826 c         9340 g        13265 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgtta
//
LOCUS       DAZKMB010000010        40933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000010 DAZKMB010000000
VERSION     DAZKMB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.010"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACG8V3_000515"
     CDS             complement(38..1189)
                     /locus_tag="ACG8V3_000515"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0299082.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACG8V3_000516"
     CDS             complement(1202..1639)
                     /locus_tag="ACG8V3_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0299083.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACG8V3_000517"
     CDS             complement(1654..3420)
                     /locus_tag="ACG8V3_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0299084.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACG8V3_000518"
     CDS             3684..4361
                     /locus_tag="ACG8V3_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0299085.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACG8V3_000519"
     CDS             4364..5491
                     /locus_tag="ACG8V3_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0299086.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACG8V3_000520"
     CDS             complement(5691..6131)
                     /locus_tag="ACG8V3_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0299087.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACG8V3_000521"
     CDS             complement(6142..8175)
                     /locus_tag="ACG8V3_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0299088.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACG8V3_000522"
     CDS             complement(8177..9880)
                     /locus_tag="ACG8V3_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0299089.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG8V3_000523"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG8V3_000523"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0299090.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACG8V3_000524"
     CDS             complement(10963..11988)
                     /locus_tag="ACG8V3_000524"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0299091.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACG8V3_000525"
     CDS             complement(12197..13066)
                     /locus_tag="ACG8V3_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0299092.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACG8V3_000526"
     CDS             complement(13525..15417)
                     /locus_tag="ACG8V3_000526"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299093.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACG8V3_000527"
     CDS             complement(15414..17141)
                     /locus_tag="ACG8V3_000527"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299094.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACG8V3_000528"
     CDS             complement(17157..17807)
                     /locus_tag="ACG8V3_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0299095.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACG8V3_000529"
     CDS             18011..18307
                     /locus_tag="ACG8V3_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0299096.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACG8V3_000530"
     CDS             18479..19099
                     /locus_tag="ACG8V3_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0299097.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACG8V3_000531"
     CDS             complement(19191..20357)
                     /locus_tag="ACG8V3_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0299098.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACG8V3_000532"
     CDS             complement(20369..20722)
                     /locus_tag="ACG8V3_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0299099.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACG8V3_000533"
     CDS             20850..21872
                     /locus_tag="ACG8V3_000533"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0299100.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACG8V3_000534"
     CDS             21891..22052
                     /locus_tag="ACG8V3_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299101.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACG8V3_000535"
     CDS             complement(22311..22745)
                     /locus_tag="ACG8V3_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299102.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACG8V3_000536"
     CDS             complement(22855..24714)
                     /locus_tag="ACG8V3_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0299103.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACG8V3_000537"
     CDS             24715..24855
                     /locus_tag="ACG8V3_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0299104.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACG8V3_000538"
     CDS             complement(24832..25992)
                     /locus_tag="ACG8V3_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0299105.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG8V3_000539"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG8V3_000539"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0299106.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACG8V3_000540"
     CDS             complement(27739..28437)
                     /locus_tag="ACG8V3_000540"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0299107.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACG8V3_000541"
     CDS             complement(28394..30055)
                     /locus_tag="ACG8V3_000541"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0299108.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACG8V3_000542"
     CDS             30273..31673
                     /locus_tag="ACG8V3_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0299109.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACG8V3_000543"
     CDS             complement(31925..33004)
                     /locus_tag="ACG8V3_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0299110.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACG8V3_000544"
     CDS             complement(33090..34049)
                     /locus_tag="ACG8V3_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0299111.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACG8V3_000545"
     CDS             complement(34061..35572)
                     /locus_tag="ACG8V3_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0299112.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACG8V3_000546"
     CDS             complement(35594..36157)
                     /locus_tag="ACG8V3_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0299113.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACG8V3_000547"
     CDS             complement(36163..37026)
                     /locus_tag="ACG8V3_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0299114.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACG8V3_000548"
     CDS             complement(37040..37936)
                     /locus_tag="ACG8V3_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0299115.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACG8V3_000549"
     CDS             complement(38111..39400)
                     /locus_tag="ACG8V3_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0299116.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACG8V3_000550"
     CDS             complement(39372..40889)
                     /locus_tag="ACG8V3_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0299117.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11794 a         8582 c         7091 g        13466 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgcc ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgt
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tga
//
LOCUS       DAZKMB010000011        39424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000011 DAZKMB010000000
VERSION     DAZKMB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.011"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..1454
                     /locus_tag="ACG8V3_000551"
     CDS             135..1454
                     /locus_tag="ACG8V3_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0299118.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1643..2044
                     /locus_tag="ACG8V3_000552"
                     /pseudo
     CDS             1643..2044
                     /locus_tag="ACG8V3_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
     gene            complement(2349..2663)
                     /locus_tag="ACG8V3_000553"
                     /pseudo
     CDS             complement(2349..2663)
                     /locus_tag="ACG8V3_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(2917..4552)
                     /locus_tag="ACG8V3_000554"
                     /pseudo
     CDS             complement(2917..4552)
                     /locus_tag="ACG8V3_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4875..5354)
                     /gene="rlmH"
                     /locus_tag="ACG8V3_000555"
     CDS             complement(4875..5354)
                     /gene="rlmH"
                     /locus_tag="ACG8V3_000555"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0299119.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            5782..7071
                     /locus_tag="ACG8V3_000556"
     CDS             5782..7071
                     /locus_tag="ACG8V3_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0299120.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(7110..7715)
                     /gene="ytpR"
                     /locus_tag="ACG8V3_000557"
     CDS             complement(7110..7715)
                     /gene="ytpR"
                     /locus_tag="ACG8V3_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0299121.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(7920..8384)
                     /locus_tag="ACG8V3_000558"
     CDS             complement(7920..8384)
                     /locus_tag="ACG8V3_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0299122.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(8406..8726)
                     /locus_tag="ACG8V3_000559"
     CDS             complement(8406..8726)
                     /locus_tag="ACG8V3_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0299123.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(8870..9949)
                     /gene="pepA"
                     /locus_tag="ACG8V3_000560"
     CDS             complement(8870..9949)
                     /gene="pepA"
                     /locus_tag="ACG8V3_000560"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0299124.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            10149..10499
                     /locus_tag="ACG8V3_000561"
     CDS             10149..10499
                     /locus_tag="ACG8V3_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0299125.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10722..11669
                     /locus_tag="ACG8V3_000562"
     CDS             10722..11669
                     /locus_tag="ACG8V3_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0299126.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(11726..13144)
                     /gene="pepV"
                     /locus_tag="ACG8V3_000563"
     CDS             complement(11726..13144)
                     /gene="pepV"
                     /locus_tag="ACG8V3_000563"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0299127.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            13293..14138
                     /locus_tag="ACG8V3_000564"
     CDS             13293..14138
                     /locus_tag="ACG8V3_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0299128.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            14142..14582
                     /locus_tag="ACG8V3_000565"
     CDS             14142..14582
                     /locus_tag="ACG8V3_000565"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0299129.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            14632..14826
                     /locus_tag="ACG8V3_000566"
     CDS             14632..14826
                     /locus_tag="ACG8V3_000566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299130.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            14962..15648
                     /locus_tag="ACG8V3_000567"
     CDS             14962..15648
                     /locus_tag="ACG8V3_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0299131.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(16056..16745)
                     /locus_tag="ACG8V3_000568"
     CDS             complement(16056..16745)
                     /locus_tag="ACG8V3_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299132.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(16720..18849)
                     /locus_tag="ACG8V3_000569"
     CDS             complement(16720..18849)
                     /locus_tag="ACG8V3_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0299133.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(18897..20234)
                     /locus_tag="ACG8V3_000570"
     CDS             complement(18897..20234)
                     /locus_tag="ACG8V3_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0299134.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(20486..20845)
                     /gene="rplT"
                     /locus_tag="ACG8V3_000571"
     CDS             complement(20486..20845)
                     /gene="rplT"
                     /locus_tag="ACG8V3_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0299135.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(20898..21098)
                     /gene="rpmI"
                     /locus_tag="ACG8V3_000572"
     CDS             complement(20898..21098)
                     /gene="rpmI"
                     /locus_tag="ACG8V3_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0299136.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(21170..21694)
                     /gene="infC"
                     /locus_tag="ACG8V3_000573"
     CDS             complement(21170..21694)
                     /gene="infC"
                     /locus_tag="ACG8V3_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0299137.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(21738..21863)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            22014..22646
                     /locus_tag="ACG8V3_000574"
     CDS             22014..22646
                     /locus_tag="ACG8V3_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0299138.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(22648..23553)
                     /locus_tag="ACG8V3_000575"
     CDS             complement(22648..23553)
                     /locus_tag="ACG8V3_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0299139.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(23638..24297)
                     /locus_tag="ACG8V3_000576"
     CDS             complement(23638..24297)
                     /locus_tag="ACG8V3_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0299140.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24613..25557
                     /gene="mvk"
                     /locus_tag="ACG8V3_000577"
     CDS             24613..25557
                     /gene="mvk"
                     /locus_tag="ACG8V3_000577"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0299141.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            25550..26527
                     /gene="mvaD"
                     /locus_tag="ACG8V3_000578"
     CDS             25550..26527
                     /gene="mvaD"
                     /locus_tag="ACG8V3_000578"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0299142.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            26540..27625
                     /locus_tag="ACG8V3_000579"
     CDS             26540..27625
                     /locus_tag="ACG8V3_000579"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0299143.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            27622..28686
                     /gene="fni"
                     /locus_tag="ACG8V3_000580"
     CDS             27622..28686
                     /gene="fni"
                     /locus_tag="ACG8V3_000580"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0299144.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(28935..29738)
                     /locus_tag="ACG8V3_000581"
     CDS             complement(28935..29738)
                     /locus_tag="ACG8V3_000581"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0299145.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(29758..30600)
                     /gene="kduI"
                     /locus_tag="ACG8V3_000582"
     CDS             complement(29758..30600)
                     /gene="kduI"
                     /locus_tag="ACG8V3_000582"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0299146.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(30616..31623)
                     /locus_tag="ACG8V3_000583"
     CDS             complement(30616..31623)
                     /locus_tag="ACG8V3_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0299147.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(31630..32274)
                     /locus_tag="ACG8V3_000584"
     CDS             complement(31630..32274)
                     /locus_tag="ACG8V3_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0299148.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(32374..33132)
                     /locus_tag="ACG8V3_000585"
     CDS             complement(32374..33132)
                     /locus_tag="ACG8V3_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0299149.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(33259..34410)
                     /gene="fucO"
                     /locus_tag="ACG8V3_000586"
     CDS             complement(33259..34410)
                     /gene="fucO"
                     /locus_tag="ACG8V3_000586"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0299150.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(34492..34803)
                     /gene="rhaM"
                     /locus_tag="ACG8V3_000587"
     CDS             complement(34492..34803)
                     /gene="rhaM"
                     /locus_tag="ACG8V3_000587"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0299151.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(34805..35650)
                     /gene="rhaD"
                     /locus_tag="ACG8V3_000588"
     CDS             complement(34805..35650)
                     /gene="rhaD"
                     /locus_tag="ACG8V3_000588"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0299152.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(35664..36920)
                     /gene="rhaA"
                     /locus_tag="ACG8V3_000589"
     CDS             complement(35664..36920)
                     /gene="rhaA"
                     /locus_tag="ACG8V3_000589"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0299153.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(36917..38377)
                     /gene="rhaB"
                     /locus_tag="ACG8V3_000590"
     CDS             complement(36917..38377)
                     /gene="rhaB"
                     /locus_tag="ACG8V3_000590"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0299154.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            38530..39399
                     /locus_tag="ACG8V3_000591"
     CDS             38530..39399
                     /locus_tag="ACG8V3_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0299155.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
BASE COUNT        11540 a         8241 c         7182 g        12461 t
ORIGIN      
        1 attaaaacca tgtgaaactg cctcacggca gcttacaatt actttttcat cttcacccgt
       61 taagtgatga aaaaagaacc cctttctgct atggttaaag tgactaaacc aaccaaaaga
      121 aaggagttct tttcatgact agcttacaca aaaaccaagt aaaattcaat tcaaatatca
      181 ctatttctca tacaggtggt caattatcga gtgattcggg tctcgtccta gtgaaagagc
      241 tgatgaacat ctttggtttt tctgaactgg ctaagcaaca cattcacatt gaagacgaac
      301 gagcgtattt tactcatgac aacttatcca tacttgagca gttcattatg caattaattg
      361 ctggttactc agctgattct gcagctaatc tcctgagaca agatcctgtg tttaaagctg
      421 ttttagatag gaaagaactc gcttctcaat cttcactttc ccgattttta gatcgactat
      481 ctgaggagaa tatccatgaa cttcaagctt tgaaccaaga acttatcgat aaagcacgcc
      541 tcatccgtaa tgatacggaa ttaatcattg atttagattc gacccattca gatacatttg
      601 gtcaccaaga acaaacgaat tataatacac actaccaaac ctatggttac catccattgg
      661 tagcttttga cggattgact ggtgacttct taaaagctga actacgttca ggaaatcaat
      721 atacatcaaa aggcgtaaag gagtttctca cacctttatt agagcactat aatcactctc
      781 taccaaacac tgacatcttg gttcgtggag acagcgggtt cgctacacct ggtgtgtatg
      841 attcgtgtga atcaaaaaag agtcattatg ttattcgact gaagaataat cgtagactag
      901 gtcaaatagc tgagaaatca gtactttatg gcgataatca aaagtgggaa gaacgagaag
      961 ttcagtactt ctcgaccact tatcaagcac aatcctggtc acaaagtcgt cgcgtgtgta
     1021 tacgctcaac acgtgaagcg ggcgaactac tctttcgaca tgaatttatc gtgacgaatc
     1081 tatcagaaaa tgtttctcct gataccatct tttctatcta tgccaaacgt ggcacaatgg
     1141 agaacttcat taaagaagcg aaagctggct tttactttga caaaacagac agtcctcgct
     1201 ttttggagaa tcatgtccga atgatgctca gcttgatagc ttacaactta gtcaacttct
     1261 taagaacgat tggctttgaa gaagtccaaa agggaatgac cattcattct attcgattga
     1321 agtttctgaa agttgctggg aaattagtcc aaacgggtag acgagtctat ctcaaattat
     1381 ctagttatca tgtgtatcag aatgaattct acagggtctt tgctcgcctg aggcgagcca
     1441 gtcaatggat ctaatccaac aatctaacga tttttttact gggaagttat ccagggagaa
     1501 gtatgctcaa aatgccttac acccaaaatt tattcctcaa tatttttgaa cagtgaatgt
     1561 ttgaagtaac tttttagtca aaatcagttg atagggatat tagtgataca tatttcaata
     1621 aaatgtgcca aagaattaaa gctatgaatt attcagggat caattagaaa gcaacttttt
     1681 tcgggatcta aatgaaattc tctcttatga tccagcccat agctatgagg aactatacac
     1741 tcgcttgatt gactatgttt acgcaaatac ttttttatat cgcttattta tcctcaacca
     1801 tggtgatatt tcgtttcaga agaaagttaa acaagttatt gaagaaaact atctgaacat
     1861 atggctatat gaagacaaga aaacgattat cacagaagaa atgagattct ttactacgta
     1921 ccacgtccaa ggttgtctat ccgttctcaa tcggtgggta gaaagcaatt acaagtactc
     1981 caaaaaagaa ttattagaac tcttacagaa actgaatgat cacatagaga aaatcatgcc
     2041 ttgaataaaa acatactaag cttagttaag aagaaccgta accttctcgt ataacaactg
     2101 aatcttattt agccaaattg tttactgaag attttttgag taaaatttct ataattccta
     2161 cttattgaaa agtagtttgt agtgctgctt ctccctctct aagtgatata tcttgttgta
     2221 ccaatcaaat tcacctcatc attaataaat tacttttagg ctaatttatt acaacttata
     2281 ggttgaagct gtaaccaatt tatagtcata ttatattttt atcatccagt ttattgaatt
     2341 cttctcgttt acctcaattc attttcaagg ggaatcagct catcatctaa cattttacgc
     2401 catgctcgct tcatttcttg tgggtattta aatggtttcg cttctggatg tttggcttta
     2461 tatccttcgt aatccttact gttgataatt gctttcatat taggaattgg atcttcacct
     2521 ggcatatatt gcagattaga agcatgtaat tttttctcag aaacaaacca ctcattggaa
     2581 aagacatgaa tcaaatcatc acttacttgt gtaaaatagc gacgtttaat gacaaagata
     2641 tcttcattgc tatctagtgt accttcattt ctttgtgctt aaattcaaag ataattttct
     2701 caataaagat ttttgattcc agtaagattc ctttactcct tttacaacat agcgttgtcc
     2761 tttgttttga aacatcttct tcccctcttt gccattaaaa atagcccaat tctctataaa
     2821 gaagattaag cttcattatt ataatataaa gtttaaattt ggtttattac ttactttttc
     2881 ttattctcta gtaagctaat attgaaaaaa atgtaatcag atcattattt ttatatgttg
     2941 taacttcctg ataagaaaac taattaaaag aaagaggtaa ctacataaac taattaaaaa
     3001 agctaacttc atagttggag gataaatgga agaaacttca atgattgttt ctccttgtgt
     3061 tccttcaatc gacactagtc ctattatcaa tctctctttt ttgtccattt acttttgcga
     3121 ctgttcccca ataatcaaat acaggaagcg ttattttttg tcctttagat tcatgtactg
     3181 tatagactat tttcgaatcc atcacttgat aaggcatagt atgccattcc ttgccaattc
     3241 ttacttcatg attctgcaaa tgttctttgt attgtaatgc ttccgtgggt gcatagtcat
     3301 aaagtcctcc gggaaggagt gtcgtgtaac gaataagatt ttcttgattc attaccaagt
     3361 gatctctctg agtgacaact ttatgttcat agtttgcttt tgttgcagca gatacagcca
     3421 acagtaataa aacaattgcc gttatattaa ctacttttct ttgcttagct gatgtgctct
     3481 tcatgaaaac gaacgataaa atcaacgagc caaacagtac ttgaaagcct aagattcgcc
     3541 aaggaaattg aatcatttga atgggtgtat tttgtaataa agaccatgga aaaaatttgg
     3601 tggataagag ccaagagact accgcactaa ggtaaatcac tttttctttg cgattaaatc
     3661 ctttaaaaaa gaagagtgga cagatcaaag aaataatacc aatgattcct accgtaaagg
     3721 ttgtcaggtc attattcatc gcaccaatca aactatccga gacatttaat gcttggtgtt
     3781 gtaaggtcgt tttatctggc tcattaatcg attgaaccat gatttgtcgg aacatcggat
     3841 accaaaagta tgctgttaaa agaatcgtca ttcctactgc ctttgacaac tccacaatca
     3901 accggatgct ttcttttttt cttgtttcac ttactacaaa gccgcctaag actagtaagc
     3961 caacggctat tacactcata aaagtggaaa gaacatgtgt ataaatgagt aagctcatac
     4021 tgactgctaa tggaattgca catggctttt tctcataaat cagtgcgtaa gcataataca
     4081 taactatcgg tataaccgta agtgcaatcg cttcagcaat agcagaacga tgataaatat
     4141 caatcgtacg gtacccataa aatgtatata aacagctaaa tataaaagcc gcatcgattc
     4201 tttttaaaaa ttttagtcca taatgaaaca tcagcatgat agtacaaaca ttcaatagcc
     4261 aaacatataa gatataacta acgattaaat tattacttat ccaataaaaa atgactgccg
     4321 gaaaaaaggt caagtaggga taaaaataat tcactccgct cccataatga ttaaacgtcg
     4381 taaaattgat tggaccagaa aaaattgaag ataacccttt gactctagca aaatggaagg
     4441 caatatcccc cgatgtagaa atagtatgaa catttctaaa ataattgatt gaataaagtc
     4501 cagcaataat tgctaggaga atggacaata ttattttttt ctttacttgc attacttaat
     4561 tatatactcc tttcatggat tgctttatct agtataactt aaattttaaa taaatcgtta
     4621 tttattttta ctctcactta aaaaagcact aaaattataa gagagggagg taatggtctg
     4681 aacaccaaga attgttccaa catcgaattg cttaactaat gagttcaaag cttaattatg
     4741 tggctcttat atttaccgag cgtggattgc gatctcttta gcgaaacaaa catctttcat
     4801 aaaaaaaaca gccatatccg ctttcttgga tatgactatt tactactact ttactatctt
     4861 tcattcttct tgtctcactt atgataaggc gcccccgcat tgatccggaa ggcacggtag
     4921 atttgctcga caaggatcag acgcatcaat tgatgtggat acgtcatttt tccaaaagag
     4981 atctgagcat tgcttctttt catgacggct tcgctgagtc ctaaagagcc gccgataaca
     5041 aaaacaaggt ggctttttcc ttggatacct aattggtcga tttgtttagc aaaagattca
     5101 cttgtaggat ttttcccttc aattgctaag gcatagacgt attcttgttc tttgattttc
     5161 gctaagatcc gttccccttc tttttctttt acttgtctca tttgcgcttc gcttaaattc
     5221 tccggggctt tttcgtcagg aacttcgatc aattcgattt ttgcataggc attcagccgt
     5281 ttgacatact cattgatacc ttggatcagg tatttttctt ttaattttcc tactgagata
     5341 attttgatat tcatgttttc tctcctttgg tcttcatgct cactttgttt attataatca
     5401 attttttcgt ctatatgttt attttggcta tttttgttaa aaagatttcc ccaacagtta
     5461 tgcacaacat ccacaggttt atccacagaa aacagtgtta ttttacctta tttcatcaca
     5521 ttcattaggc aaacatacgt tttataagaa tttatccact ttatacacag gctgtggata
     5581 tgttttatcc cttatttttc caatcctatc cgcgtagttt tacacagtat ctgtggaaaa
     5641 agaaaatcaa atattttcat ctgaaaacaa tgatttttcc taaagttttt cataaaaagt
     5701 tcacagtctc aaagtagact aaagtcaaca aataagacta tactaacagt agataaatga
     5761 atgtaattgg aggaggaaca catggataga aaaaatgtga caccaaagat gaaaaaaagt
     5821 aaaaataata gcctctggcg taagttaggt ctggggcttg ttggagggat cgttggagga
     5881 ctactgacag ctggcatttt ttatgcagcg atgggcactg gcaacaatag ttctactgct
     5941 acaagcggca atcagaattc tgccggtgaa acagttgtgg aaaatgtcaa agtcaatgta
     6001 gattctgata tcaccaaagc ggtagataaa gttcaagacg ctgtcgtttc tgttatcaac
     6061 ttacaaagtc aaaaccaatc aagcggtttt ggcggactat tcgggcagca agaagaaagt
     6121 agcagctcag atgatagtaa tttggaagct tatagtgaag gaagcggcgt gatctacaaa
     6181 aaagacggga atactgctta tgtagtcacg aataatcacg tagtagatgg ccagcaaggt
     6241 cttgaagtct tgatgaaaga cggtacgaag gtaaaagctg aattagttgg aacagatgct
     6301 tattcagatt tagcggtgtt gaagatcaat tcagacaaag tcgaaacagt tgcctctttt
     6361 ggtgactcaa gtgctctgaa ggttggtgag cctgcgattg cgattggttc cccactagga
     6421 tctgaatatg caaattctgt cacttcagga atcatttctt ccttgaaccg acaagtgaca
     6481 agtacgaatg aatcgaacga aacagtcaat atcaatgcga tccaaacaga tgcagcaatc
     6541 aaccctggaa attctggtgg tcctttagtt aatatcgaag gacaagttat cggtatcaac
     6601 tcaagtaaga tcgcaagtac ttctgaatct acttctaatg taagcgtaga aggaatggga
     6661 tttgctattc caagtaacga tgtagtgaat atcatcaatc aactggaaaa agacggaaaa
     6721 gtgacacgtc cagctctagg tatcacaatg gtcgacttat ctgccgtttc tactcagcaa
     6781 caagaacaaa tcttgaagat tccttcatct gtaactaacg gtgtcatcgt gacaaatgtc
     6841 caaacagcta caccggctga aaaagctgga ttgaaacaat acgacgtcat tactaagatc
     6901 gatgacaaag aagtttcttc tggtgtagaa cttcaatctg ctttgtataa gaaaaaagtg
     6961 ggagattctg tcaaaatcac ttactatgaa ggctcgaaag aaaaaacaac tactgttgaa
     7021 ttgacgatcg atcaatcggc attaaaacaa aatcaaaata gtgaaaatta ataaaaaaga
     7081 ggttgtgaca aagacttgtc acaacctctt tattttggaa atgcttctcc gactacggca
     7141 tcctcaggca atactaagat tccttttttt gctggagcat ttggcaaatg caattcacga
     7201 gcggaacaga tcattccata gctatctacc cctcgcaaag ttcctggcca gatcatcatt
     7261 ccatctggca tcatagctcc tggttttgca acgacaactt tttgtccagc ttcgatattg
     7321 ggtgcaccgc aaacgatttg caatactgta ccattgtcta cttctgtctc agtaatcgat
     7381 aaatggtcag aatcaggatg tgggacgcat gtttttacgt acccgacaac gatcttcggt
     7441 tcatgatcag cttcaagctg ttcttcgaat cctgcttctt tgatcaatcg attcaatttc
     7501 gctaattcct catctgttaa gcttacttgg ccgttccctt cgattgttag atgatcggag
     7561 atatggaaaa agttccaagc aacaacttga ccttgttcat ctttgatccg agcaacattc
     7621 catttgcgtt caacagtgtt ttctgttcct ttatcgtcgg ctataataac catcaaggtg
     7681 tctccgacgt gtgctttgtt ataagcaaaa atcatgttct ttcctccatt atcctttgct
     7741 gagatttatc ttaacaaaaa attgcattca ctgcatgaaa tttttttcat gctttatttc
     7801 cgaagaagcc tcttctggaa caccatttat tcgtaacaag ggactgtgac aagacttttg
     7861 tcacagtccc tttactgttt tagttaattg attaaaaagg tcgtacaagt tccacttgct
     7921 taattacttg gctgttcttc ttcgtcagaa acgatcaaga catcacaagg tgaatgacgt
     7981 atcacgtagc tggctacact tcctgtcata aagcgttcga ctgcatttag acctgactgc
     8041 ccaaccatga ttagatcgat ctcatatttt tttggcaatt tgactgccat ggattcacga
     8101 acagaaccat atgcaataat tccttctata tttgtgtaat caacagattt cccatattct
     8161 ttacattctt cgatcatttc cttggctgtt tcagtttgct gatcgactag attttgatca
     8221 ataggtgcat agcccataaa gttataaaat tgctgatcga ttacgtttgc tacatatact
     8281 ttcccattat tacggcgagc cacttctacc gcttttttga aagcagcaag tgcttgttca
     8341 ctaccgtcga tacctactaa aatattctta tattcttgat tcatcttgac ccctcctaac
     8401 agatgtcagc cttttgttac actttctagg aacgattcga tttcctgttt tgttttacga
     8461 tctttattca ctagacgacc taattctttt ccgtcttgga tcacgacaaa acttgggata
     8521 ccaaaaatgt tccattcagc ggctacatct atgtacttgt cacggtctac ttcaataaat
     8581 tgccaatcag aaaagtcatt ttcaatttca ggcatttgcg gtttgataaa acggcagtct
     8641 ccgcaccagc cggcagtaaa gaaaaataca ctctttccag tttctacgta gcctgctaat
     8701 tcttctaaac tatttgggat aatcattctt tgtcctctac tttctgttta tgtatttttc
     8761 aattaaatta tacacctttc tttcgaacta acaaacattg atcctctttt tataaaaaaa
     8821 atctggcaaa aagcaatctt ttgccagatt ttatagtttc actatcgcat tattttccgt
     8881 atacgatcgt atttactgtt gatttatcca aacctttcag tgtttgaatc aacatttctc
     8941 gtgcagcttc aaaatcgcga atactgaaca tcgtttgatg cgtatggata taacgtccca
     9001 ctacgccaat cactgtactt gggattcctt cgttttgtgt atgagcagca cctgcatctg
     9061 taccgccttt tgaaacaaag tattgataag ggatattgtt tgtttcagcg atatccagca
     9121 agtactcgcg caaacgagga agcatgatca aacctggatc ttggatacgc atcaatgtac
     9181 cttctcctaa atgtccgaat gtgccatttt tcgtcactgt atcatctgct gcagagcagt
     9241 ctactgcaaa gaacagatct ggcttgaatt tattgacaga agctttagaa ccacgcaagc
     9301 ctacttcttc ctggacattc gcgcctgcaa tcaatgtatg acctaatgtt tcattttgca
     9361 acgcttctaa agcttctaat accattgtac agccatagcg gttgtcccat gatttactga
     9421 taatattctt tccgttggct gttttgatcg tttcagcgta aggaacgatt gtgtctcctg
     9481 gaaggacacc gtaactcatt gcctcttctc ttgattcaaa cccagcatcg aacaaaatat
     9541 cggttacttc tactgatttt tgtccgcttg ttcctcgcaa taaatgtggg ggaacggaag
     9601 aagaaataca tggataattc ttaccagtag aggttttaag tgtaaaacgc tgagcagaaa
     9661 caacgtaagg attccagccg cctagaggta cgactttaaa aagcccgttt tcttggattt
     9721 gtgtcagcat gaatccgact tcatccatat gtgctgcgac cattacacgg ggtgcatccg
     9781 cttcttcatg atggcgcaga ccaaaaatgc cgcccaaacc atctaactgt aactcatcta
     9841 ccagcggttc catatgactt tccatataag cgcgaacatc ttgttcaaaa ccgcttgttc
     9901 cttgtaattc tgttaactct ttgattcgtt gaaatgtttt ttcttccata tgctattcgt
     9961 ctaaatttca gactaaactc ctctcagact tatattgatc tgcggataaa atatgatttt
    10021 ttctgcagac cgatcgtatt tattatagct tattttttct ggtagcggtt atttatttca
    10081 aagaaaatat gagagtgtgg taaaataagc acaaggagta acaagtgaga agaaggaggt
    10141 atgtccacat gtatgaagaa agcgaactaa gctattttaa aggcgggtta gcagttggaa
    10201 tcggtctagg attattgagc ggggtagctt caacgatttg gtatcatcgc aaaaaagtat
    10261 tgtcagcaga ccaagtactt gaaaatatca agtcatcttt tctaaaagaa ggtgcaatcg
    10321 aaggttcttg gatctctttt gaaaaggaac ctattaggaa attcgccgtt cggtcgagtg
    10381 cttatcgggg aggtctttct cgaatagaag atggcgaaac tgtttattat gaatttttag
    10441 cagatgctca tacaggaacc gtattagaaa tttcaagaaa aaaaggtgca acagattaat
    10501 acgtcaaaaa agtgagagaa cgaatacgca cccactattc aaaagagagg tatcatgtcc
    10561 ttcttttgta gtgggtggtt ttttagatag agctttccaa acatatattc cagtattttc
    10621 tttttaaata aatgatattt agcgggaaaa actagcattt cctttgatta tgaggtattt
    10681 ttaatttagc ttttgttttc tatagttaag gagagtgaaa aatggaacaa caagaaaaaa
    10741 ttgctgttct gattccttgt tacaatgagg aagcaacaat ttcaacagtc attgcagact
    10801 ttaaacgaga attaccagaa gcagatattt atgtctatga taataattcg accgatcgaa
    10861 catacgaatt ggccgttcaa ggtggagcaa tcgtaaaaaa agaacctcgt caaggaaaag
    10921 gaaatgtgat ccgccaaatg ttctttgata tcgatgccga ctattattta atggtagatg
    10981 gcgatgatac ttatcctgca gaagctgtac atggtttgct cgagaaatta cgttctggtg
    11041 aagcagatat ggtcattggt gatcgcctat caaatggaac ctactttgat gagaataaga
    11101 gacctttcca cgattttgga aataaccttg ttagaaatac aattactaga atgtataaaa
    11161 caaaaattct tgatgtcatg accggctatc gaggattcaa tcgtatcttt gtcaaaagtt
    11221 ttcctattat gagttctggt tttcaaatcg aaacggaatt gacgatccat gctttagata
    11281 aaaaattcaa gttcgttgaa atcccaatcg attatcgtga tcgtcctgaa ggcagcgaat
    11341 caaaattgaa tacattctct gacggattca aagtgatcat gatgattatt aaaatgttta
    11401 aagattataa gcctttgctt ttctttggga tttggaccat cttcttcttt ttatttggat
    11461 tggtcacggg gattcctgtg attcgcgaat atatgctgac aagctttatt acacgcatcc
    11521 cttctgcgat tttaagtact ggattgatga ctttagcact tctttcatta gtaacagggt
    11581 tgattttgga tacagtcgtc acgaacgcta agaaagaata tgaattgaat ctatatcatg
    11641 tttatcatga ataccacagt agaaagtaaa aaaaggatca agactgcaaa aataatttgc
    11701 aattcttgat cctcttttta attaattatt tcgccaaacg atagatggca tctgcataga
    11761 ttgctgttgc tctgaacaag tcatcaagac tcataaattc gtttgcttga tgcattgtat
    11821 cagtatatcc tgggaacatc gctccataag caacgccacg tttcaacaaa cgtccgtaag
    11881 ttccgccacc gatgatttgt tcgtagcctt tttctccagt gtggtcttca tagacttgca
    11941 ataatgtagc caccaatgga tcatccattg gtacgtaatg tggttccatt acgcgagcac
    12001 ctcgagtaac tgttgcgcct tcttgcccca ctgtttgttc gatgtcactt tgcaatccgt
    12061 ctactgttac accttttgga aaacggaaat tcatattgat gaagttcgct tcctctgtac
    12121 cattttcttc aaaagcaaat agtccagcat tcattgtcaa tttgcccatt ttttcatctt
    12181 cataagcgac tcctagtttt tctccataaa aatcttcatg gacataagca gctgctacat
    12241 ggataaattg tttagcagat cctaggaatt catagttatc taagaacaat gccaagaaag
    12301 aacctgcatt gataccagat tgtgggcttg caccatgtgc gcctttacca actaattcga
    12361 tttttacata cgtattgtcc gcttcaatcg ttccgcttac tggatttgct tcgatgaatt
    12421 ggtaaaatgc ttcttccatc gcgatagctg catctgcact tggtacttga agtgctgcag
    12481 ttgctgttcc tggcaccata ttttcgcgaa gtccggaaac aaaagatttc aagacataat
    12541 ctcctgcatt gtcgcctttg aagcgtaaag ctaaagaaac atttcctttt tcaccattga
    12601 tgattgggaa ttcagcatct ggtgagaaac caaaatctgg ttcttcttca tgttcgaaat
    12661 aataagccat atcgccccag ccgctttctt catcgctgcc gactacgaag cgaacttttt
    12721 ttgatagttt caagtcaagt tctttaatga tcttcatcgc atagtaagct gccatacttg
    12781 gacctttgtc atcgcttgat ccgcgtgcaa agatctttcc atccttgatt actggttcat
    12841 aaggatctgt gtcccagcca tctcctgcag gaacgacatc catgtgtcca aatatcccta
    12901 gcgtttcgtc tccttcgcca agatcaatat gccctgcata gttatcgaca ttttttactt
    12961 taaatccatc acgttcgcca taagctaaca tatgtttcaa tgcgtcgcga ggtccaggtc
    13021 caaatggtgc atctggtgtt acttttgagt catcgcgctc gctgttgaca cgtaaaagat
    13081 ttttcaagtc ttcaagcaag tcttctttac gtgcttctac ttctttttgc caatcaattg
    13141 tcataaagtt aatcctccac ttatttaagt cttcgtgttc atgataccac aaattacaga
    13201 aaaaaatagt tcaaaaaatc tgacaattcc ttttcttatc tgtttttctc taaaaaaaat
    13261 atacgctata atgagtaaaa ggaggttttt agatgctgat aaacgaacaa ctagtgaaaa
    13321 gagtcatcac acctcgtcca aaaatttcac ataaaggaac atttggacgt atcgttttga
    13381 ttggcggcaa tgaacaatat ggcggagcaa tcattatgag tgctttagct gccgtacaca
    13441 gcggagcggg tctcacgact gtagtaactg atgaaaaaaa tcggagtgct cttcacagtc
    13501 atctgccaga agcaatgatt gtagtttgga cggatactga ccagtttctt tccgtaataa
    13561 agcaagcaga tgtactcttg attggtcccg gattgggtac ttcttctaag agtttggaac
    13621 gtttcaaaca agtactagaa aaccaatcaa aagaacaatg gctggtgatt gatggctctg
    13681 ctttaagttt acttgctgct aatcatcttt tgctacccta tcccgaacaa actgtactta
    13741 cacctcatca aatggagtgg caacggttga gttctctacc gatacaagaa caacgggata
    13801 aagaaaacaa aaagaagcaa caggaaatcg gtgctgtgct cgttttgaaa agtcatgaga
    13861 caaaaattta tggtcaccag cacttctaca ccaatccttt aggttctgct gctatggcaa
    13921 cgggcggcat gggtgacact ttagctggga tgatcacagg atttttagcc cagtttcctc
    13981 aaaatgacca aacgattgca gcagctattt atcttcatag tttcattgga gaccagctgg
    14041 caaaagaaca atatattgtt ttgccgacac aaatcagcga agcacttccg aaatggatga
    14101 attatttttg taaaaaatca atggaaaacg aggcatgagt catggagaaa tttacaggtt
    14161 atgtgcgaga acctttttat tatgaaacag atcaaatggg gattattcat cacagcaatt
    14221 atatccgctg gtttgaagag gctcgggtag ccttattggt acatctaggg tttccctata
    14281 aaaaaatcga agaatctggg atcatcatcc ccgttctgga agtaaattgc agctataaag
    14341 aaatgatcca ttacggagaa aaagtgagca tccatccaac tatccaaaag tatacgggga
    14401 cacggttgga ttttagctac gaaattgtgg gaactgaaga caaaaaatta aaaactactg
    14461 gcagtagtaa acactgtttt ttactttctg attcgcaacg attagtgaag ctgagcaaag
    14521 tcaatccgga gttagatcag ttattccaag cgtacgcttc tttggatgat gtaaattctt
    14581 aataaagacc gtctaagcat ttttcttgta ttgtgtcgag tagttcgtat aatgaaaaga
    14641 aaaaagcagg tgatccccat gtatgtcgtt aaagtaatgc acggttacat cgataaaact
    14701 ggctgtcgca ctcgggaaaa aaacttagac aatctgctca ttttcaaaga caaaaaagaa
    14761 tccgaagctt ttgctaaaag aatcggcggt cgtgtcaaac cgatccaaga ggtacgacca
    14821 gattgattca atatttttct tttcgaggct ctaataggca tgcctatcag ggcttttttt
    14881 gcagactgtg tttttatgcc cacacgattt gtaagcgatt tatgttataa tagattatat
    14941 ctgcatagaa aggaaggtta aatggttatc aataatttgc aacagcaagc ttatgaatct
    15001 atacgtaaac aaatcattta ttccgagtta gcacctggaa gtaaaatctc cgataaagtg
    15061 ttagaagaac aactagctat cgggcgtaca ccgattcaag aagcactgat ccaactgcgc
    15121 aatcaagagc ttgtaaaaac cattccacag tccggcactt atgtttcaaa aatcgatatc
    15181 cgatctgctt cattagcccg ttatacgcgg gaaaagttgg aaaaaccgat cctgcaagaa
    15241 tgcagtgcga aaatgaccaa gcaagatcaa gctaaactag aaaaaatact agaacaaacc
    15301 gatcaggcag taatcgctca aaataaaaaa gctttttttg atttggatac tgattttcac
    15361 cgaacttgtt atgaaattgc tggaaaacga gaaatttggg attggctaga aagttatagt
    15421 acgcacttga accgtttcag atggttacgt ttgacgattt cagaattaga ctggggtcga
    15481 gtattagatg aacatcagac aatgcttcaa tcaatgattg atcataactt tgatgaagtc
    15541 ggatttcttt gcacgatgca tttacatatg attatcgagg agcaagaata cgtgattcat
    15601 aattaccccg attattttga agatattcag gacagaccca tcaaataaga ggttttggaa
    15661 tcagcgtttt gacctgagta ttagagaaag aaacggcaaa atagttcctc atattttgcc
    15721 gttttttttg agctaatacc gagggtcagc tgattgcaca ccgtttattg agaggttgtg
    15781 aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaatagctt cttatgtttt
    15841 taacttggct ggacttatta ccgcagatgc aagcttttga acaccgtttt ccaagaggtc
    15901 gtgagcctgc cgttcatctc cgagtcacaa ggaacaattt caaaaaacag cttctcatgt
    15961 ttgttgaata ttttgacttg tgaccgagga gttgacagga gaacatcgtt tattcggaaa
    16021 aaaaggactg tgacacgact tttgtcacag tctctttatt tgtaatctaa atcatactcc
    16081 gtacatcttt tttctatttt ttttatttct tctaagatcc cttggctacg aatttcatta
    16141 tatggccaaa gtaagactgc ctctaataca taaggcgaaa aatcgctttg attcatctct
    16201 tttgaaaaac tcatcgctgc tatctcgctc aatgcacgaa tgtttgattg atgtgtataa
    16261 aaaggcaacc gccacagcac aattttttcg ctttgcgtat aaattccctt aacagaagct
    16321 tcgatgtggt gaaatagttc atgtccaata atcaaagctt gcgctgtttc tttagagatc
    16381 gaagctagtc ctgaacattt taatttttct aacggctccg tcatcaatcg aatcccttca
    16441 tcttctgcat aagttgcaaa agtgattcgt tcgtttattt gctctgattc acaggaaacg
    16501 attggaacag ccatttgttc acaaatttct cctacatcgg caaaagggta cttttttttg
    16561 atgctacatg ccgtttcgat accacaatcg attgattgtt gtataagaat tttctgcttg
    16621 gacaaaggaa tctttttcca aaataaatct cgccgaaact ggtagaatcc ccaaatttct
    16681 tcggggatct gttgtaattc gtttagtaac tcttctaact tatgtttgtt tgatatttcc
    16741 ggcaacgacg atcacttctt cctccggttc catagtagat acttccagat ccatcaaaag
    16801 ctgtagttgt tccagatccg tcgctgtaaa atcagagact cttgctccta agcagagata
    16861 aaattctggt cgggcaatca attcagtttg ataaactgcc atatcttccc aaagttgttc
    16921 gacagctgtc atataattgg ctgcagttgt tttcatacat gaagcatgtc cacgttggac
    16981 cttgataaat cctttttgat cgatgatccg aagacttcca gcattttttg aacgattctg
    17041 ttcgccgcat acataaaaga aaggtgtatt ttccaataag caaacttcat ttttatttaa
    17101 tcgcatatcc tctgcagcaa gctctagtgc ttcttctgtt gtgatctcct tggttagatc
    17161 ggttgctttc acttccgtag aaccagtagc gatagccgtt acttttgaag tctgtggatc
    17221 gatttctacg tggacttcga tcgattctgg tgtagcgcct gattcaatcg ctttattcat
    17281 cgcttcattt ttcaacgatc gtatatcttc tttacttggc gaagggatga tacgttctac
    17341 aacatctcgg accattgcaa gtgctacacc aatagaagaa atcacttcgg cgttttcagg
    17401 aatcgaatat ttcacaccca ttttattact aaagtaagta atcaatgagg cagcgccgcc
    17461 ccctacacct acaagagaaa tctgatcttt ctccaagtga tacttgtctg ccaattctaa
    17521 aatcaccggt tcaatcttag cataggattt ttccatgatc tgttctgcga tgtcttcaac
    17581 cgtcgtatga caataatcag ctaatgcttg gatggctttt cgtgcagatg ggacatttcc
    17641 ataagaaaaa tgttcttctt gcactaatcc aaggacattg gctgcacatg tatttgtaat
    17701 agtcacttct tctccgtctt ccatcactac cttgacgtaa tcagcagggt ctccttcttt
    17761 aggagagaaa aattcaacct taggaccttt gattttttct gtctccgtaa atactgcata
    17821 atctagaccg gcaatatgtg cagaacgtgg accgacatcg atgattcctg actgattagc
    17881 ccgtaccatc gaaccgcctg ccacacctag aacccgaaca tctaatgaag agatatatgt
    17941 tgggtggcca cctacaattg aataatcgat tgctggacgt ccatttttaa tgaccccgat
    18001 attggttgtt gttccgccta cttcaaaata aacaccatta gacgctctca agtacatcaa
    18061 agagcccatg actgatgctg ctggaccgga aagcatggtt aaaacaggtc gttttttcat
    18121 ttcattgatt tccattactc cgccgtctcc gcgcataatc atcaaagaaa cattgacccc
    18181 tgcttctcgt acggaatctt ctgtagaggt tgccgtatct agcatttttg gtaaaataga
    18241 tgcattgatt gctgctgtcc gagttcttct tgtcaatcca tataattttg tgatgtcaga
    18301 agccatcgtt gtagggatcg attttaccga tgctgcttca taaacaactc gttctggttc
    18361 cccattatct actccaaatg ccatagaaga aaccaagacc tccgctcttt ctctttctaa
    18421 agaagaaatc gtttctgaaa cacggtctac atttaagtgt ttgacaggta agaatgcatt
    18481 gacaatctct atttttttct tattccctaa atcgatatcg ttcaatctag tttgtcgttt
    18541 tgctaaaaaa ccttctaatc caccttttgc catcccgatt acgccgactt ttgctacgtc
    18601 tccttcaatc agtgcattag tcgcttgtgt ggtagaatgt gcaacaaaca caacatcttc
    18661 tggactgata ttattttcca gcaaacaatt ttgaaaagac tgaacgacac ctgcggctac
    18721 tccacggaca tcatcgtggg ttgttttcac tgaagatttt ccgatgattt cgtgcgtcgc
    18781 attatcgatt gcgactgctt tcgtatgtgt accgccaaca tcaattccca ttcgcacaaa
    18841 acgtttcatt ttcttttcct ccattcatct aatcgttttt atcgacgttt tgtttactag
    18901 gcgttcccgt acaataagaa cgtgatcagg cataagatcg ctccgatgat ccaaccagtt
    18961 gggatggatt tcttcatata ttctcgagaa gttacttttg tataacctaa tccccaagca
    19021 acccaagatt gtgtaatatc taaatgctga ggagcaatcg ttgtgattgc aaataacggg
    19081 aacaaaaagg ctggcgacat cgttggatta acagctaata cgacagctaa aatagctgag
    19141 cctgaaccga ctagattcat tggaccgcgg aaaaatccta gtggtgtcaa tatagcaaat
    19201 acgattgcca agactaatgg tgattgcggt atccaatctc caaaaatagc tgaaaaataa
    19261 ggagaagcat acgcagctga attattgaac atcgctaagg tcatcaagaa accaaccatt
    19321 ggcgcaacat ctactgcgcc atctgtaaat aatttcgcaa actgacgaca gatttcttta
    19381 taacctccgc gcaattttcc agtaaacagc aatgcccaga tacctgctag aataaaacct
    19441 aagatgattg gaagatctaa aagaacgacc cctaaaactg gtaataatac ggctaaccat
    19501 gagatcattg gtgcgtcgcc accttcagct ggtacgtttg ctgccattgc atagcgtttc
    19561 tttttgttca ttgatatatt agcgaccgtt agtaccacaa ctaagcttac aatcatccca
    19621 atcataccaa tttggaaata gtcattataa gtatagcttt cagccgctgg attgaaacca
    19681 gcataaatcg tttgatattg tttgaaattg acgatattag caaagattcc agccataatc
    19741 gagcccataa atgagaacag tgcaaccggt actgaaatac ctaatgacat caagattggc
    19801 aacacaataa ctgcaataga aatcactggt ccgattcctg tcatcgacat gaatataaca
    19861 gcggttacaa tacatagtaa gctcatcgtg attcttggct tgtcccctcc aagttcaact
    19921 acctttcgaa tcagggtagc tgcgattccg ctatctacta gaacacgccc gaaaaaggca
    19981 ccaaagaaga catttaccaa gatcgatttg gcgtactcag ctggccccgt cgtatagata
    20041 tgcgtcaaca catcaattac tccttgccct tccatcgccg ggtttggtga aaacgtgttt
    20101 ccgactagcg ctaatcccat ccatacagta gccatgatgg caaagccgac catcaagttg
    20161 tagcctttta cacagtacca gaccatgaaa aagaatgaga taactaacaa aataccaata
    20221 ataatatcac tcatgttatt cctccgtcca ttttgttttt ctgataagca aactaatata
    20281 tcagtcaaac tcattctaac ttatgtagtt agcgctttca ttaaagggtg accataaatg
    20341 ttcagttttc aattaaaaaa ccttaattat gctattttag acttatacgt tattgtcctg
    20401 tttcatttga tggaggattg taaataaaaa aagaggaaag aacataactt ttcgttatct
    20461 tctttcctct ttataattgt ttagcttatt tagccaaagc ttcttttgct tggtcagcca
    20521 atgcagtaaa tgctgctgca tcgttaacag ccaagtcagc taacatttta cggttgatgt
    20581 cgatttcagc aactttcaaa ccgtgcatta attttgagta gctcaagcca ttcatacgag
    20641 ctgctgcgtt gatacgagca atccacaatt tacggaagtc gcgtttcttt tgacgacgat
    20701 cgcgatatgc gtagttgtat gaattcatta cttgttcttt tgctgtttta aataaagtgt
    20761 gttttgaacc gtagtaacct tttgctaatt ttagcactct tttacgacgt ttacgagtta
    20821 ctgttccacc tttaacacgt gccatgtata attcctccta ataaaaataa atcttcagat
    20881 tctgttattt gtacttctta tttcattttt gctagttgtt ggcgaatacg tttgaagtcg
    20941 ccactagaaa ccatgcttgc tttacgcaat tgacggcgtt gtttttttgt tttgccgtgg
    21001 aaacggtgac ttgtaaacgc gcggaagcgt ttcaatccgc ctttaccagt acgtttgaaa
    21061 cgttttgctg atccgcggtg tgttttttgt tttggcatga ctatatttcc tcctcaaaat
    21121 ctttcgttat ccgactatcg gctgtccaac agccagttag tcattcaaat tacttactgt
    21181 cgtttttcgg tgccagcgtc aagaacatgc tgcgtccgtc cattttcgct ttttgttcca
    21241 ctgttgcaat atccgcagtt tcttcagcta agcgattcaa gactttttga ccaatttctt
    21301 tatgggtaat ggcacggcct ttgaaacgga tagaagcttt cactttatcg cccttttcca
    21361 agaacttacg tgcattacga agttttgtat tgaagtcatt caagtcgatc gtaggactta
    21421 agcgaacttc ttttacattg atcacttttt gttttttacg cgcttcgcgg tctttctttt
    21481 gttgttcgaa acggtattta ccgtagtcca tgattcgcgc aactggtggt ttcgcaccag
    21541 gggcaactaa cacaagatcc aagtttgctt gttctgcaat attcaatgct tctgcttttg
    21601 tcttaactcc taattgctca ccatctgaac cgatcaatcg taactctcgt gcacgaatgc
    21661 cgtcgttaac catcatatcc tttgctatgg tcattcacct ccaaatatta tgagagaaag
    21721 aaaagcttac cgaataaacg gcaataaaaa aacgagttct cttacagaac ccgcgcgtac
    21781 ttcacctaat agaaccagtg aaaactatct agcccagtga cattatcaac taaggcgaga
    21841 agcgggtgct tctgctttta tttctcaacc atcatagtat agcaaaaact aatctttttg
    21901 tcaatgtttt tcatacagtt tgtcttcctt ttctgaaaat gattagatta gcagactttc
    21961 ttgcagtgat tcccgcttct ttgctatact aaagaaaaac gaggtgaacc cacatgaaaa
    22021 aaatcgaacc tattccacct tctcttcctc ttcttacaaa gggagagttt tatttagaag
    22081 atgaggagat cttctctggt cttctgatca acgggggtga ccaaagctat ctcgatgcca
    22141 aaaatatggt attaagagag agtcatatcc aaaaacttac gatgcaaaaa acagttttac
    22201 gtcattttga atgcagtaat gtcatttttg aaaaatgtga tttttccaat ctggagtgga
    22261 ttggtggcag tttccaccaa gtcgtgtttc atcaatgtaa attaacaggt acgaattttg
    22321 ccgaaagtta tcttcgagac tgtactttca ctgattgtat tgcttcgatg gcttccttta
    22381 gtagtacaaa cttgaaagct gtctccttcg atcattgtca attagaagac agcgaatttt
    22441 atgaggtcac ttggaaaaac ctttttttgt ctgacaatca gttgaccggt tcgaactggt
    22501 ttcgaacttc tctgaaggga ttagacttta cgactaattt cttcacagca atcgctttat
    22561 ctcaagattc tttggcggga ctaatcgtca accaagagca agcacttgtg atcgcccaag
    22621 cccttggttt gcagatcaaa gactagatca acgaatccgc cgccacagtg caataagcat
    22681 ccaaatagat aagaccaaac tgatgatcaa ggcgattccc catccgaaaa cggaatgggt
    22741 aaagggtaac tggagattca ttccgaagaa tcctgtcaca atagttggaa cggtcaagat
    22801 cagcgaccag accgtcaaaa atttcatcgt gtcattcaag ttgttattca gcaaattatt
    22861 ataagtgcca gagagctggt ctaaaatctg cgaagctaag ttcgtcattt caactgcttg
    22921 tcgtgcttcg atcagcgcat cgtccaactc ttctttttcc ttttctgaca acttgcgata
    22981 aatagccaat gccttgattt gttctaaaag tactacattt tgttttgttc ctgtaactaa
    23041 gtaaaccaaa ccgatctcta aatctgaaag ttgcagcaga ttcttgttcg ttgttttttc
    23101 tcttaatttc agattaagcc gctgacgttc actattcact tcctcgatta aaggaaaaaa
    23161 ggaatcagaa atcaaaaaca atgtacgaaa caataaactg tatacactca tgtctggcat
    23221 tcgttccaaa agcgatcgaa ttagtcgaat cacgtactcg gtattcggcg tggtaaatgt
    23281 aaacaaaccg tcttctttta aaataaaagc gataggacta gtttcataat gattatcgac
    23341 tttttgctgg tgcggaacat ttgatacaag cagtaacgct tcatcaaaag aatcgtactc
    23401 tacgcgcgcc cgttcatttt tatctagaga ataagtcagc atctcgtcac taatgccata
    23461 ttcttctttt aagtttttaa cagctagcac atcgttttgc tgtacattga tccaaaaatt
    23521 acggtcttga ttaaaatagt gtttttcccg catactctat cctcctgaca tttgtttcca
    23581 gtataaagaa aaaagtcgct caaataaagg tttacccttt atttgagcaa ctgtaaatca
    23641 aatggctact ttggtttggc gaatctctgc taatttttct tcgatcattt tcaagacttc
    23701 ttcttttgct tgaggatctt ccacaaaatt atagcggtct ccatcgattt ggatttttgg
    23761 actttctttg tattcttcat accattgatc gtaacgtctg ttcaattctt tgtaataatc
    23821 gtatagactt ggatcaaagg acaattgttc atacgaacga ccacgttttt cgatacgctc
    23881 caacatcgta tcaaaagata cacgaatatg aactaataaa tccggatgtt ttttatgggc
    23941 agcataggga agttcttcca tcatgttttc cagcagttca tcatacacac gaacttctgt
    24001 ttctgttgct cttcctagat ctgcatttag atggaaaagc agtgaatctt cataaatcga
    24061 acggtccaat acattattgt catctttcat tgcttgtttg atgcttgcaa aacgtttatt
    24121 caaaaaataa atttgcagca aaaaagcata ctgttcaggg tttgcataaa ataagggcaa
    24181 gacttcatta tcatcaatcg attcataaaa tgcctgacta tcaaagtgtt cagccatcat
    24241 ttctgttaaa cttgattttc cggcaccaat tgtacctgct aaaacgatca cgctcattta
    24301 cctccagctc acttcatcac taaatattgt gtttaactcc aaccttttta aatatacccc
    24361 caatatctag tagtgtcaat tgtatttttt tctagtcaaa gaagaaaaaa agtcaaaaaa
    24421 ttcttaagaa aaaagtgttt tttctttaga aaacgtattc ttagttttac ttctgagatg
    24481 gttttagtaa aataaaggag gaatgttttt tgagaccctt agtggacaaa aaaattcgtg
    24541 aagcagcgca actcgttttg ggcgctttat tttttgctta aaaatcactt ttacgtgaga
    24601 aggagttaga ttatggcaaa ctatggccaa ggagaatcaa gcggaaagat catattgatg
    24661 ggcgagcacg cggttgttta tggagaacca gcgattgcct ttcctttcta tgcaacaaaa
    24721 gtcaccgcat tccttgaaga gctggatgca atggacgatc aactggtttc ttcctactat
    24781 tcaggaaatt tagccgaagc tcctcatgca ttaaaaaata tcaaaaaatt attcattcac
    24841 ttaaaaaaac agcatgacat ccaaaaaaac ttgcaactga ccattgaaag cacgattcct
    24901 gctgaacgtg gaatgggatc aagcgctgca gtcgccacag cagtcactcg tgctttttat
    24961 gattacttag catttccttt gtctcgtgaa atactattag aaaatgtcca gctttcggaa
    25021 aaaatcgccc atggtaatcc tagtggaatc gatgcagccg ctactagcag cttgcagccg
    25081 atttatttta caaaagggca tcctttcgac tacttttctt tgaacatcga tgcttttttg
    25141 attgtcgctg atacaggaat caaaggacaa acaagagaag ccgtcaaaga tgtcgctcac
    25201 ctctttgaaa cccagcctca tgaaactgga caaatgatcc aaaaattagg atacttgacg
    25261 aagcaagcaa aacaagcaat cattgaaaat tcaccagaaa cgttggcaca gacaatggat
    25321 gaatcacaat cacttctgga aaagctgaca atcagcaatg attttcttaa tctattgatc
    25381 caaacagcaa aagataccgg ggcattgggt gccaaattaa ctggcggtgg acgcggtggc
    25441 tgtatgattg cattagcaca aacaaaaaca aaagcccaag aaatcagtca agcacttgaa
    25501 gatgcgggtg ctgctgaaac ttggatacaa ggattaggag tacataccta tgtttaaagg
    25561 caaagcacgc gcatatacca acattgctct aatcaaatat tggggtaaga aaaatgaaga
    25621 actcatcctt ccaatgaaca acagcctttc gttaacattg gatgcatttt atacagagac
    25681 agaagtcatc ttttctgata gctatatggt agatgaattt tatctagatg gcaccttgca
    25741 agacgaaaaa gcaacaaaaa aagtcagtca gtttcttgac ctttttcgta aagaagccgg
    25801 cctctcacta aaagcttcag taatcagtca gaatttcgtt cctaccgcag ctggattagc
    25861 ctcctctgcc agcgggctag ctgctttagc aggcgcttgc aatactgctc ttaagcttgg
    25921 attagatgat ctctctcttt caagatttgc tcgacgcggg tctggttcag cttgccgaag
    25981 tattttcggt ggtttcgtcg aatgggaaaa aggccatgac gacttaagtt cttacgctaa
    26041 gccagtccct tccgattctt tcgaagacga tttagcaatg gttttcgttt tgatcaacga
    26101 ccagaaaaaa gaagtgtcca gcagaaatgg gatgcgtcgg acagtcgaaa catccagttt
    26161 ttatcaaggc tggttagatt ccgttgaagg ggatctatat caattgaaac aagcaatcaa
    26221 aacaaaagat ttccaacttc tcggagaaac gatggaaaga aacggactaa aaatgcatgg
    26281 aacgacttta gctgcccagc ctccattcac ttactggtct cctgacagct taaaagcgat
    26341 ggatgcggtg cgacaattga gaaagcaagg cattccatgc tactttacga tggatgctgg
    26401 ccctaatgtc aaagttttag tggaaaacag ccatttatct gaggtacaag aaacatttac
    26461 caaattattt agcaaagaac aagtaattac tgcacatgct ggtccaggaa ttgcgattat
    26521 tgaataaagg gagattttta tgattgaagt atctgcacca ggcaaactct atattgccgg
    26581 agaatatgca gttgttgaaa caggccatcc agcagttatc gctgcagtcg atcaattcgt
    26641 gacagtcact gtagaatctg cacgaaaagt cggaagtatc caatctgcac aatatagtgg
    26701 gatgcctgta cgttggacaa gacgcaacgg agaattagtt ttagatattc gggaaaatcc
    26761 ttttcattat atccttgctg ctattcgctt gactgaaaag tatgcacaag aaaaaaacat
    26821 tcttttatcc ttttatgatc tgaaagtaac aagtgagtta gacagttcaa atggccggaa
    26881 atatggattg ggttctagcg gtgccgtgac cgttgcaacg gtcaaagcct tgaatgtttt
    26941 ttacgcgttg aatttatctc agttggagat tttcaagatt gcagcactag ccaatttagc
    27001 agttcaagat aatggttcct gcggcgacat cgctgctagc tgttatggtg gctggatcgc
    27061 tttctcaacc ttcgaccatc cttggctcca agaacaagaa actcagcatt ctatcagtga
    27121 gttacttgcc ctggattggc caggtctatc cattgagcca ttgattgctc ctgaagattt
    27181 acgtttattg attggttgga cgggtagccc tgcctctact tctgatttgg tcgatcaagt
    27241 tcaccgttcg agagaagata aaatggtggc ttatcagctt ttcttaaaaa atagtacaga
    27301 atgtgtcaac gaaatgatca aagggtttaa agaaaataat gtaacgttga tccaacagat
    27361 gattcgaaaa aaccgacaat tactgcatga tttatctgca atcactgggg tcgtcatcga
    27421 aacgcctgct ttgaacaaat tgtgtaattt agctgaacag tatgaaggag ccgcaaaatc
    27481 ttctggtgca ggtgggggcg attgcggaat cgtaattgtt gaccagaaat ctggcattct
    27541 tcctttaatg agtgcatggg aaaaagcaga aatcactcca ctgccgttac atgtctatag
    27601 cgatcaaaga aaggaaaacc gatgaatcga aaagacgaac atgtttcact agctaaagcc
    27661 tttcatgata aacaaaaaaa cgaatttgac ttcgtacgga tcatccataa ccctttgccg
    27721 caaatagcag tttctgatgt cgatctaagt acacaggcag ttgggtttac actaagcagc
    27781 ccattttata tcaatgcgat gactggcgga agcgaaaaaa caaaaaaaat caaccaagat
    27841 cttgcaatcg tcgcaagaga agcagatttg atgattgcca ctggttcagt cagcgctgcc
    27901 ttgaaagtcc cttctttagc cgatacttat acgattatgc gacaagaata tcctcacggg
    27961 aaaatcattg ccaacatcgg tgcagggact tctgtcgaaa gagccaaaga agctatccga
    28021 ttgtttcacg cagatgcctt acagattcat ttgaatgcac ctcaagagtt ggttatgcct
    28081 gagggagacc gtgattttac caattggaaa gtacttattc aagaaactca aacagccatt
    28141 gacgtccccc tcatcgtaaa agaagtcggt tttggcatga caagagaaac gctcaatgat
    28201 ctagctgctt taggagttca tacagttgat atcagcggtc gaagcggaac aagcttcacc
    28261 cagatcgaaa acgcccgtcg ctccaagcgg gaactgagtt atttagctga ctgggggcaa
    28321 tcgacagtct cttcattgct tgaagcaaat gaagcagaca cttctatgga aatcctagcc
    28381 tccggaggta tccgcaatgc ctatgacata tttaaagccc tttgtctcgg cgcaaatgcc
    28441 gtgggtactt caggaaccgt actgacgcat ctgatgaatc acggcgtaga agaaactatt
    28501 atactgatga agcaatggca agaagagctt cgtctgcttt atactatggt cggcgcaacg
    28561 aataccgcgg ccctacacca acaatctctg atcttttccg gaccagtgaa ggattggtgc
    28621 gaagcaagag ggatcgactt agtgaagtat ggaagacgca cagaaaagag cgtgggacaa
    28681 aagtaaagaa cacttttgtc tcacgctcta aacacgtaca aacggcggaa gtaaaagcaa
    28741 ctccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaattcctt
    28801 cttatttctt ggagttaaat gcttctgtcc cagcctcttt tttcgaataa atgatgttta
    28861 atcagctgtc cttcggtatt cgttcaatcc gctgatttca caacctctcc actaaacagt
    28921 gtactaaagc tcttttaagg ttgttttccg atataggcaa gtatgcctcc gtccacatac
    28981 aagacatggc cgttaacgaa atcagatgcg ctagaagcca agaagacaga tggtccagcc
    29041 aaatcgattg gctcgcccca gcgtgctgct ggcgtacgtc caacgataaa ttgatcgaat
    29101 ggatgacgtt caccgttttc ttgtgtttcg cgtaatggag cagtttgcgg tgtagcaata
    29161 taacctggtc caattccatt acattggata ttgtattgac cgtattcaga agcgatgttt
    29221 ttcgtcagca tttttaatcc gccttttgcc gcagcataag cactgaccgt ttcgcgtcct
    29281 aattcactca tcatggagca gatattgatg attttaccgc cgcctttttc gatcatatcc
    29341 ggaataactg cttttgaaac gatgaatggg gcatttaaat ccacgtcgat tacttggcgg
    29401 aagtctgccg catccatttc gatcatcggt gtccgtttga tgatccctgc attgttgact
    29461 agaatatcaa ctgaacctac atcttctttg atctgttgaa ccatcgcttg aaccgcttct
    29521 tcatcggtca catcgcagac ataacctttt gcatcgatgc ctgcttcttt ataatgcgct
    29581 ataccttcat caactgattc ttgattcaag ttattaaata cgatttttgc tcctgctgta
    29641 gctaaagagc gtgcaatctc aaatccaata ccatagacag caccggtgac taatgctact
    29701 tttccatcta aacgaaacat atccatacta aattccatca ttttctctcc cttatgctta
    29761 ttttaattga tccatttgca ccatatccat gtctgtatag gtgatatttt ctccgcacat
    29821 tgcccagatg aatgagtaat tgcttgtacc tacacctgaa tggatcgacc agcttggcga
    29881 aataatggct tgttcattgt ctacaactaa atgttttgtt tcatctggtt tgcccatcat
    29941 gtggaacaca cgtgtatctt cattggcata atcaaagtag acataagctt ccatacgacg
    30001 ttcatgtgta tgacaaggca tcgtattcca agcgcttcct ggttctaaaa tcgtataacc
    30061 catttgtaat tgacagcttt cgcatacatt tggatggatg tattggtaga ttttacgttc
    30121 attcagtgtc aggccttcac cagtctccat tggttttact tggtcgatac tgatttttac
    30181 atttggatat ttatgatgag ctggaacaga actaatatag aatttagctg ggttttcagc
    30241 atcttgagaa gaaaatacaa catgtcgtgt ttcttttccg atatagtatc cgtcttgttt
    30301 tttcatttct tctttttgcc catcgatctc gatgaatcct ggtccaccga tattgatcac
    30361 acctagttca cgacgctcta agaagtaatc aacacctagt tctttgtcta atgtgatttc
    30421 taattctttt tcagtaggtg tcactccacc aaagatcatt cgatcgttgt gcgtataagt
    30481 caaactgatt ttccctggtt caaatacctt ttctactaaa aattcttttc tcaattgttc
    30541 cgttgaataa tgacggatat cttctggact gtgtgtataa cgtgtttcca tttcttgcat
    30601 tttcttctac tccttctatc gaatgatttt tccagagccg catgctaaaa attgtgcgac
    30661 ttcttcttga ctaaattggt tgcagtcccc atggatcgta tgcttcaagg cagaagctgc
    30721 cgtagcaaaa tcaatgattt cttgcggaga catattggat aaaatcccat ggagcacgcc
    30781 acctgcaaaa gcatctccgc cacctactcg atccacgatt ggggtgattc tatggacttg
    30841 ggattccaca tattcatggt tcatccacaa tgtccccttc aattcatttt cacttgctga
    30901 atgaacggta cgcttggtcg aataaaacac ctgaatattc gggaactttt cctgcatttt
    30961 ttgataataa taaattagag gttgttcttc tgcttctggc gctttctcga tacctaacaa
    31021 ataaagagca tccatttttc cagctgagca gatatctact aaaggtaaaa gctgatggat
    31081 tacttcgctg gcttcttgct gtgtccataa ttttccacga tagttgatat cgaagctgat
    31141 caggcagcca gctttttttg cagcttctat cacctgtttc gtcatttttt gccatttttt
    31201 agacaaagct ggggtgattc ctgaaacatg aaatatatct attccttgga ataactcctc
    31261 tagcgaccat tccaaatgat ccatttcagc aaaacttgat ccggcacgat catagatcac
    31321 tgaggcggcg cgttcgccac taccagtttc catataataa gtacctagtc ttgatccacc
    31381 agataatacc tgtttcgtat ctactccata acattgcaag tgctttttga ctgcttttcc
    31441 caatgcgtta tcaggtactt ttgtagcaaa aatcgcttca tgtccgtaat tagctaaaga
    31501 aattgctacg tttgcttccc ctccgccata atgggcagcg aatcgttcac ttgaatcgat
    31561 acgggttccc tgctctgtgg acaatcgcaa catgatttct ccgagtgtca ctacttttcc
    31621 cataagcagc tacctcttga tttctttcat ttttgctgca tactgttgcg cgacttccgt
    31681 tactttgtca aaatctcctg tagctgcggg tgctaaaagg ttcccaccta ctccgactgt
    31741 tacgacacca gcatcgaacc actctttcat attgtccaga ctcacaccgc cagttggcat
    31801 gatattgact tgaggcatcg gcgccttgaa ggcagaaatg atacttggtc cataagcgct
    31861 tccaggaaat agtttgacga tatctacccc gcttttcagc gctgttttga tctcggtaat
    31921 agtcatacat cctggtaagt aaggaatttg atacagattg cacatctctg ccgtttcttg
    31981 atcaaaggaa gggctaacaa tatactctgc gccagctaag atagccaagc gagctgtgac
    32041 agcgtctaat actgtgcctg cgccaatcac tatttctggt tggtctttgt agaatgacaa
    32101 aagttctttg attacttgat ctgcttgagg gactgtaaaa gtcaactcaa taccagttag
    32161 accacctttg atgattgcgt ggcaagcatt caaagcttct tctttgcttt ttccgcgtac
    32221 caccgcaatc actccggcgt ttttcaatcg agataaaata tcaatttttt tcatcttctc
    32281 cacctatttc ctatttagaa attttatttt ataacttgga aatctatacc catattatac
    32341 ggcagaaaag aaaaattgca aacgcttttt tcattatttt tgaattcttt gctcgatttc
    32401 gctttttgtt tgcttgaggg cactgattat ttgttctttt gtctcatctg ttaaacgata
    32461 tttcggcata cttacactaa atgcaccaat gatttgttcg tttttcacga ttgaggctcc
    32521 tatacaaaaa atatcacgtt cgatttcttc atcgtcaaat gcgatgtggg tttcttttac
    32581 cgttttgatt tcctttttca accgtaatgc attggtgatc gtatattctg tgtaagggat
    32641 caacggatgc ttttcaagat aatcagtgta ctgctcttca ctgaattctg ctaatacggc
    32701 tttgcccatc gcagaattgt ataatggtcg ggtgattccg atttttgaag acatgcggat
    32761 cgtttgatgt tttggatcta gtttgttcac gtacaagatc tcattgttgt ccaatacccc
    32821 taagtggata gtctcatcga tcttttgctg cagctgttct aaaaacggta aagtcaactc
    32881 taccaaatcg atttgttcaa tgtttttatt cgcgtagcga ataagctttg cacctaaacg
    32941 atagtttttc tctgaatttt tatttacgta gccgatcatc aacaaagtat ctagtatttt
    33001 caagactgta gaagaggtca ttcccacacc ctttgcgatc gtctgcaaag agacatcttg
    33061 gttttccgct aaatagtcta gtattttaga ggctttgatc aatacagttc cataaggctt
    33121 tttattttcc atataatatt ctcctatttc tttataagaa aaattatagc ggaaatacca
    33181 tcaatttaaa agagatatcc agatataaac taccatttct ctttacaggc aatttaatgt
    33241 tgaagatccg cttcgttttt acatcaaact ttgatataaa gcaatgattt cttctactgt
    33301 cgtttctctt ggatttcctg gtgtgcatac atctcgcaaa gcatcttctg caattgctgg
    33361 aatatctgct tcttttacac ctaattcaga aatggtttct gggattccaa ccgctttgct
    33421 caaccgatcg atctcaccac aagccgcttc tcttacatct tctaaagaca tttcagctgc
    33481 tcctttaatc cctagaacaa gagcgatctc acggtacttt tctccagtat attctttgtt
    33541 atatttcatg acagttggca aaagtgctgc acacgctacg ccatgaggaa tgttgtacca
    33601 tgcacttaac gggtgagcca tcccgtgtac cagtcctaaa ccgacatttg agaagcccat
    33661 tcccgcaata tactgtccta atgccatttt ttctcgccca ttttgatcgc catctacaga
    33721 agcttccaaa gaatggccga tgatttcgat tgcttttaaa tgcagcatat cagtcatttc
    33781 ccaagcgcct tttgtgataa acccttcaat agcatgcgtc atagcatcca tacctgttga
    33841 tgcagccaat tttttcggca ttcccatcat catatcgctg tcgatgaacg cgacgattgg
    33901 gatatcatga ggatcaacac aaacaaattt tcggtgattt acttcatctg tgatgacgta
    33961 gttgatcgtt acttctgctg ctgttccaga agtcgttggt atagctaata taggtaaaca
    34021 tggatttttc gtatcagcaa caccttctaa actaatgacg tctgaaaact ctggattcgt
    34081 caaaataatc ccaatggctt ttgccgtatc gattggcgaa cctccaccga ttgcgatcaa
    34141 acagtctgcc tctgcctctt taacaaaagc cactccatct tttacatttt ggatcgttgg
    34201 attaggtacg atcccgtcgt ataaagcata agcgattgct gctttatcta gaagttctgt
    34261 tacttttgtt gctataccgt gctcgatcaa tcctttgtca gtaatgacag ctgctttgcc
    34321 gaatcctcgc tgctgaaatt cagggacgat cgactcaatc gctccttttc caaaatacga
    34381 tgtttcattt aaaatcattc gattactcat tttctattcc tcctaatttg tattattttt
    34441 cacaataaaa acgaaagaac cccaagcaac cggttgccca gttgcttgtt gctacagttc
    34501 aaagactggc atcaattccc aactgactgg agaggcatct ggatttgtct ccatgatatc
    34561 ttccatgtac ttccaccatt tttgattgat agatgtattt gccatttttt cccataactt
    34621 ttcatcttcg atcactaagt aaccaaataa caaacttgta ctcggatcaa gatagatcga
    34681 atagtcgatg accccgtgtt cacgaagtgc tgtacgcatt tctggccaaa gttcatcatg
    34741 tctttttttg tattcttctt gtttttctgg gtaaactttc atgcaaaaag cttttttcac
    34801 catgtcagac acctgctttc gtatgaactt cctccaaata acctgctttt ggagtgacac
    34861 caaacgcatc tgctaaatgc cacaggtttt cgtccgtgat tgtttgcaag attggtcctt
    34921 gcgctttgac gtacgtatag acttctgctg ccttttctgc tgtctcgatc aaaccaaaga
    34981 cttcatccat gtcttttcct gacccgtaga ttccatgttg tggccataaa accaatcgag
    35041 tttctttcat tttttcagct gttgcttccc caatctcatt tgttcccgga accatccacg
    35101 gaatgatgga aattccttct ggaaaaacaa ctaagcattc cgtacacatt tgccatagcg
    35161 tacgactaaa ggaacgttcg tctaattcat gcgtaaaact catcgctaat agatgactag
    35221 catggcaatg cataacgatc cgattatctg ggtctacttc taaacgggcg atatgactca
    35281 tgaaatgact tggcaactcg cttgtaggaa ggcattcgtt ttccagcccc cacaaaagtt
    35341 ccagtgtttt gccatcttca gcaactcgaa cgattccaag attctcggca ggttcgctcg
    35401 ctacattttt aaaatatctt cctgaaccag ttacaataaa atagcatcct gctaaacggc
    35461 tggcatcaaa aatcatcgag atgcggcgga tcactttttc aggatccaag aacggtgtga
    35521 tctcttcttc ttttagtaaa taagaaatat tcccgccatt tctttcatcc catcccaagc
    35581 gataaagatt cgacgtcgtt tcgatcattt ctttcacata aggcgcttgt aaaatatcta
    35641 tttttttcaa tatagccaca ctcctatttt cttaattcca acacgtcttt ttgatattcc
    35701 tggacttctt ccagccaatc caaaccaact ggaacttgat ttttgtaaca gaaataattc
    35761 catacatcgg catacgggaa atctttcaat tcttccgtat aagccattct tgaagtaaag
    35821 tcaaaagcca gctccatttc tttcaatcgc tcaactggtt ccagcatagc tttcagcagt
    35881 gctttttgtg tatttctcgt accgatgacc caagcagcga tacggttgat cgttgcatca
    35941 aagaaatcca gaccgatatt tgttttttcc aacaattcgt tacggactaa ttctttagcg
    36001 atttcctgca attcatcatc catcaaaacc acatggtctg aatcccaacg cactggacgg
    36061 gaaacatgca acatgattcc tttgctgaat aaagctaatg aagataattt attggaaatc
    36121 acttctgttg gatgaaaatg tccagcatct agacaaacaa gtttgtcacg agtcaacccg
    36181 taacccatat aaaattcatg ggaaccgact gtataagctt cagcgcctaa accaaatagt
    36241 ttgctttcta ctgcatcttg tacataccgt tcatctaact cttctgtaaa aatttcatcg
    36301 agtgattcca ttaaacgttc ccgtggggta aaacgatcaa tcggattatc tttcatccca
    36361 tcaggtaccc aaaagttatt gatacttgtt tgcccagttt ctttcccaaa gtactccgcg
    36421 attttgcggc ttcttttccc gtgttcgatc cagtattcac gaacttcttt atctggatga
    36481 gccaatgtaa agccgtcttt catcattgga tgcgaaaaga aagtcggatt gaagtcaaga
    36541 ccgatttctt ctttttttgc ccactctacc catttttcaa aatgtttcgg ctcaatttca
    36601 tttagatcga cagattcttc tgtatccaaa tagatagcat gaaggttcaa tttgtgtttg
    36661 cccggtatca acgaataagc cttctccaga tcttgtctaa gctcttcagg tgttcgtgcg
    36721 gctcctggaa aattccctgt cgccatgatt ccaccggtca attcgccatc cggtgtcaag
    36781 aaacctttga catcatctcc ttgccagcaa tgcatagaaa ttttgatgtc ctgtatcttt
    36841 tctagtacag catctgtatc tacaccgata gttgcatatt tttcttttgc ttgttcgtaa
    36901 tttctttcaa tcgtgttcat acgattactc ctttgatttt attcacgtac cattccatcg
    36961 tgttttgctt attttcatct gggtaaaaag tttcacatgg aaacgactgt ttgattacat
    37021 ttcttgcttg ttctatattc gaaaactgac ctgtactgag catttgcatg gcaacattcc
    37081 cgattgctgt ggcttcacta ggtcctgctt ctacaatgat tccggcataa tctgctgtca
    37141 attgattcag tagacgatta ttcgatccgc cccctacgat gtgcagcttt tccaatttcg
    37201 ttccagttaa gtgttctact ttgatcagct cttgtgcgta acaaagagca agattatcat
    37261 acacacaacg agcgatagct cccgatgaga cagggactgg ctgctctgtt tcaaaacagt
    37321 attgctgaat ctcttctatc atatttgggg gattcaaaaa tcgagggtca ttgatttcca
    37381 tcacttgctg cagcggtttt tcttttgcag caagctctgc caattccgca taactgtact
    37441 cgtactcttg cattcgggca acttcctgga tcaaccacat ccccatgatg tttttcaaaa
    37501 aacgcaccgt gttatgggca ccccattcat tcgtataatt ttcagaaaaa gcattgcgag
    37561 aaacattggc gatctttgtt tcaatcccta aaagcgacca tgtcccactg ctgatataac
    37621 cccaacgttc tccttgacct ggcgttccta atacagctga cgccgtatca tgacttgcta
    37681 ctgtgataaa ggtcacatct ggcaaatcat attttgaaaa ttgttctttt tgcagatctc
    37741 ctagtatcgt tcccggctct gtcaatggtg gaaataattc cttcgagaca ccgatttttt
    37801 caagaagttc tatttcccat tcttttgtcc ctgcattcag cagctgcata gtagatgcat
    37861 tagttttctc cgtcaccatg ttaccagtaa agacataccc taaataatca ggtatcagta
    37921 acaatttatg tgcttttgcc aaattttctt tttcttcagc aaacaattgg aacaaggtat
    37981 tgaaaggctg gatctgaatc cctgtctgct catacaactt atctagagaa tagatttcag
    38041 aaaactgttc tactgctcca ttggtacgct tgtcccgata agcaatcgga tcatttatcc
    38101 gatccccttg ttcatcaatc agacagtaat cgactgccca tgtatcgatc cccgcaaaaa
    38161 ggcgatcaat ccctgtctgc tttaattttt caaatccaat caaaatctct ttgataagat
    38221 aatcgatctt ccagcgatcg tgaccgtctt tgttccgaaa gccgtttttg aaccggtgta
    38281 cttcttccaa gatcaaagaa ccttttgcat ccctcgtact tttcattagt cgtccgcttg
    38341 atgcaccgat atcgatggca acaaatgttg gattcatttc tccacctctt ttactttatt
    38401 gttgattagc actttattaa taaagcgtta tcatttattg acaatgctat cataatacag
    38461 aatcttatat tttgcttcac actattttgg tatataattg cattatcttg acaagttggg
    38521 agtgtttaca tggattactg ggaaaaagcc aaccactcat ggtcggaaga ctcaaaaaga
    38581 gtgatcttga cgcctacaca aagaagcagg aatttatttt tttatatcca agaaatcggt
    38641 tacttcaaag caccaatccc ttactatact gaacgagcac atttaccttc ttacttgata
    38701 aagtttactt tgtctggcaa aggttcgctt acatataatg ataatcagca acagctaaaa
    38761 gctggagact tattttttat tgactgccaa gaatatcagc gttatcaaac gatcagcgaa
    38821 gaaccatggg aaatggattg gatccatttg aatggtcaga acgtccaatc attttatcag
    38881 gagttcacaa aagacggcca taacaccttc cattcaacag gagatccatc agaaaacaag
    38941 atccaccatt tgatcacaga attgatccac atgcaacaag gcacgaatgc ccgtaccgat
    39001 ttccagtcct ctgtagctat ccacgagttg ctgaatgaac tgattttgca aaagtatcag
    39061 ctagatttcg aagaagagga tattcccgcc tacgtttaca aactgaagca atacttagac
    39121 gaacatttca aagaaacgat tacacttgat caattagaac aacaattctt attgaataaa
    39181 tatcagctga gcaaagattt ttccaaattt atcggttctc caccaatcga ttatttgatc
    39241 agcaaaaaac tttcttacgc taaagacctt cttcggtata cagaatttac ggttcgaatg
    39301 atttccgaag aaatagggat tgagaatttt gcttacttta gccgcctgtt caaagcaaaa
    39361 acaggactat cgccgagaga ctttcgtaaa ttcagctgat tcatataata tgtacgtcat
    39421 tttt
//
LOCUS       DAZKMB010000012        38288 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000012 DAZKMB010000000
VERSION     DAZKMB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.012"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..163)
                     /locus_tag="ACG8V3_000592"
     CDS             complement(<1..163)
                     /locus_tag="ACG8V3_000592"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0299156.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINAL"
     gene            complement(494..727)
                     /locus_tag="ACG8V3_000593"
     CDS             complement(494..727)
                     /locus_tag="ACG8V3_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0299157.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(742..1692)
                     /locus_tag="ACG8V3_000594"
     CDS             complement(742..1692)
                     /locus_tag="ACG8V3_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0299158.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1689..2531)
                     /locus_tag="ACG8V3_000595"
     CDS             complement(1689..2531)
                     /locus_tag="ACG8V3_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0299159.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(2533..3252)
                     /locus_tag="ACG8V3_000596"
     CDS             complement(2533..3252)
                     /locus_tag="ACG8V3_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299160.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            3800..4090
                     /locus_tag="ACG8V3_000597"
     CDS             3800..4090
                     /locus_tag="ACG8V3_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0299161.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
     gene            4162..5388
                     /locus_tag="ACG8V3_000598"
     CDS             4162..5388
                     /locus_tag="ACG8V3_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0299162.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(5476..5868)
                     /gene="rpsI"
                     /locus_tag="ACG8V3_000599"
     CDS             complement(5476..5868)
                     /gene="rpsI"
                     /locus_tag="ACG8V3_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0299163.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5884..6312)
                     /gene="rplM"
                     /locus_tag="ACG8V3_000600"
     CDS             complement(5884..6312)
                     /gene="rplM"
                     /locus_tag="ACG8V3_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0299164.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6846..7313)
                     /locus_tag="ACG8V3_000601"
     CDS             complement(6846..7313)
                     /locus_tag="ACG8V3_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0299165.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7433..8971)
                     /locus_tag="ACG8V3_000602"
     CDS             complement(7433..8971)
                     /locus_tag="ACG8V3_000602"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0299166.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9051..9737
                     /locus_tag="ACG8V3_000603"
     CDS             9051..9737
                     /locus_tag="ACG8V3_000603"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0299167.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9938..10465)
                     /gene="msrA"
                     /locus_tag="ACG8V3_000604"
     CDS             complement(9938..10465)
                     /gene="msrA"
                     /locus_tag="ACG8V3_000604"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0299168.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10549..11226)
                     /gene="pgmB"
                     /locus_tag="ACG8V3_000605"
     CDS             complement(10549..11226)
                     /gene="pgmB"
                     /locus_tag="ACG8V3_000605"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0299169.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11223..12353)
                     /locus_tag="ACG8V3_000606"
     CDS             complement(11223..12353)
                     /locus_tag="ACG8V3_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0299170.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(12353..14536)
                     /locus_tag="ACG8V3_000607"
     CDS             complement(12353..14536)
                     /locus_tag="ACG8V3_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0299171.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14533..15558)
                     /locus_tag="ACG8V3_000608"
     CDS             complement(14533..15558)
                     /locus_tag="ACG8V3_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0299172.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15603..16430)
                     /locus_tag="ACG8V3_000609"
     CDS             complement(15603..16430)
                     /locus_tag="ACG8V3_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0299173.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16427..17494)
                     /locus_tag="ACG8V3_000610"
     CDS             complement(16427..17494)
                     /locus_tag="ACG8V3_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0299174.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17520..18356)
                     /locus_tag="ACG8V3_000611"
     CDS             complement(17520..18356)
                     /locus_tag="ACG8V3_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0299175.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18353..19222)
                     /locus_tag="ACG8V3_000612"
     CDS             complement(18353..19222)
                     /locus_tag="ACG8V3_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0299176.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19292..20575)
                     /locus_tag="ACG8V3_000613"
     CDS             complement(19292..20575)
                     /locus_tag="ACG8V3_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0299177.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20589..22274)
                     /locus_tag="ACG8V3_000614"
     CDS             complement(20589..22274)
                     /locus_tag="ACG8V3_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0299178.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22549..23574
                     /locus_tag="ACG8V3_000615"
     CDS             22549..23574
                     /locus_tag="ACG8V3_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0299179.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24169..27822)
                     /gene="rpoC"
                     /locus_tag="ACG8V3_000616"
     CDS             complement(24169..27822)
                     /gene="rpoC"
                     /locus_tag="ACG8V3_000616"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0299180.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(27901..31518)
                     /gene="rpoB"
                     /locus_tag="ACG8V3_000617"
     CDS             complement(27901..31518)
                     /gene="rpoB"
                     /locus_tag="ACG8V3_000617"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0299181.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32246..33214
                     /locus_tag="ACG8V3_000618"
     CDS             32246..33214
                     /locus_tag="ACG8V3_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0299182.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33232..34137
                     /locus_tag="ACG8V3_000619"
     CDS             33232..34137
                     /locus_tag="ACG8V3_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0299183.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34134..34940
                     /locus_tag="ACG8V3_000620"
     CDS             34134..34940
                     /locus_tag="ACG8V3_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299184.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            35097..36065
                     /locus_tag="ACG8V3_000621"
     CDS             35097..36065
                     /locus_tag="ACG8V3_000621"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0299185.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(36382..36906)
                     /locus_tag="ACG8V3_000622"
     CDS             complement(36382..36906)
                     /locus_tag="ACG8V3_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0299186.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            37193..>38288
                     /gene="scm"
                     /locus_tag="ACG8V3_000623"
     CDS             37193..>38288
                     /gene="scm"
                     /locus_tag="ACG8V3_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0299187.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTS"
BASE COUNT        11128 a         8007 c         6931 g        12222 t
ORIGIN      
        1 taagcgcatt aatatgcggc tgaataattt cttcaaataa cgcttcctta gaagtaaaat
       61 gtttataaaa agcacccgta gttacgcctg cggtcaggca cagttgcctt atcttagttt
      121 gctgaaatcc ttctttttca aaatggctta gtccactttc tattaacttt tggtgagtta
      181 taatataatc tctggtttct atgggtactt cctcctaata agatgaatta actttattat
      241 cttacacagg acttgaagtt tcaatcgtta acgtttatta ctgaactcat tccttagagc
      301 taaactaatg agaaagagca gaataactga tacaaaaagc aatcctacaa gaattaatag
      361 actcatctaa atcattcacc ttttctaaaa taatccgaga aagatttaaa aaaattatat
      421 ccagctatta ggataagtcc tatcataatc actccaatag ccattttctt atcctcccta
      481 aaaataaagt cttttactta aaaccttttt tgtaatcgtc aataaatttt ttccctttat
      541 cagttaattg aaatcctata tctaaatagt cctcatatct aagtttagcc agcatctcac
      601 ttacagatgc tggactaaca cttagaactt ttgctaaatc attatttttt aatttctttt
      661 ttctttttga taattcctga attgctagta aataaattag tatagtagtc gttttaattt
      721 gtaccatacc taccctccaa attatttcga aaggccttca gcaattttat ccaggttcca
      781 tttcatcatc gcatagtaac tatcaccatc ttgtcctttt tctgcaattg aatcagtaaa
      841 aatcgttgaa tagataggaa tattggtatc ttttgatact gttttcattg gtctatcatc
      901 cacactgctt tctacgaata aggagggaac ttttgttgtg cgtaattttt caactaagtg
      961 ttttatttga tctggtgttc cttcttcttc agtgttgatt tcccaaatgt aagcagaagg
     1021 cacattatac gctttcgaga aatatttaaa gcatccttca cttgtaacaa tcattttttt
     1081 atcattcgga attgaagcaa atttagattt agcttctttg tctagagaat ccaatttttc
     1141 aatatactta tctagatttt ctttatagaa ttttttatta tcaggatctt tttccgctaa
     1201 ctgtttttca atatttttag cgtaaataat accgttttct aaatttaacc aagcatgagg
     1261 atcttccttc cctttctcac tctgaccctc taagtaaata acatctatgc catcacttgc
     1321 tgcaaaatag tctttgtttt cctctttgtt cgcattttta actaattttg taaaccaagc
     1381 atttcctcca gtttccaagt taacaccgtt ataaaaaatc aaatctgctt ttgaagtttt
     1441 ttgaacatct tcaggcaaag gttcatattc gtggggatct ttcccaatag gtacgatact
     1501 gtgtaaatca attttatctt ttgctatatt ttcggtaata tctgctaaaa tcgaattagt
     1561 aactactact tttaattttt cgttcgaagc cactttatct ttgtctttac tatttattga
     1621 acatgccccg aatataaaaa gagcaatcaa aatccccaga cttcccagta ctgctttcca
     1681 ttttctcatc ataacgatct ctccttcttt cttaaaaatt tttgttttgg tgagattaga
     1741 aaactaatta caaataagaa tgccgctgtt aaaacaatac ttgatcctgc tgctatgtta
     1801 aaactataac caataaacaa tccaagcaat gatgaacttg ctccgactgt tgcagaaata
     1861 aatatcatag ttttcaagct atttgcatat aaatatgctg tagctgctgg tgtaattaac
     1921 attgcaacaa ttaatatagt tcccacactt tgcatagctg ttactgctac taaagttaat
     1981 agaaccatca atagataatg ataaaaactg acattcatcc ctattgcttt tgccattaat
     2041 ggatcaaaag aagtgattaa taattcttta aaaaatagaa ctactataac caacactaaa
     2101 accgaaacgc caattgtaat ccacttatca atatcttgta cagctaaaat atttccaaat
     2161 aaaatatgaa ataaatccgt tgagctattt gcaactccta taaggataac tcctagtgct
     2221 aaaaaagaac tgaaagtaat accaattgct gtatctcctt ttattacact attgcttttt
     2281 ataaaagtaa taatgataga agctaataga ccaaatacaa tcgcaccaat aaaaaaatta
     2341 atacctaaaa tataagaaat tgctactcct ggcaaaacag catgagaaat agcatcaccc
     2401 ataagagaca ttcctcttaa aataataaaa caacctactg ttccagaaac aatccctata
     2461 actacagatg taattagagc attctgtaaa aagtgaaagt catacagacc attaataaaa
     2521 ttttgaagca tattattccc cctctccaat aaatatagta tctccatagg ctttttttaa
     2581 attttcttca ttaaatacag attctacact tccgtgagca attagatttc gattcaaaag
     2641 aataatatta tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt
     2701 ttttccctga tgtttcaatt tttttaacgt atccataatt attcgttcac taactaagtc
     2761 tatccctaca aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc
     2821 taaaagtaca cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt
     2881 tcccatatcg accttttcca aagcctttga aactctttgc cattctgcat ttttaattct
     2941 ttgaaaaact ttcattccgg catatgttcc catagataca cattctttta cttttattgg
     3001 aaaagtaaaa tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt
     3061 gatatcttca ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt
     3121 aagcaatgtg gattttccag caccatttgg accaataata cctgttaaag caccttcttg
     3181 tatttttaaa gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt
     3241 cacttctaac ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac
     3301 aaaaattata ttttagtcaa ccctaaattt tatagtttag atttttttct gttgctgaag
     3361 attagaatga caaaactatt tgaaactacg cgtatagtaa gtagctttca aaatatataa
     3421 aaaaggagcg ttctcaaatg agaacatgct cctaaaacta ctttattggc gtatggaaaa
     3481 attatacttg aagtggatag cgggttgaat aagttgataa gcatcaaaac atttataaaa
     3541 attattcaag actatgtctg ttccatatac aaatatgaaa atcagtaaac tccatttaac
     3601 ttttttcgta gtgtgtcttt ttatacatgt ttatcgttat taatagtgaa taggaaaaaa
     3661 actatttttg atcattgaca attaaataca atttttgaat aacgatccct gctattcgag
     3721 ctagaaactc aagtacgcca tgacctgatt agcgacaaat acttagagat ttctttttgt
     3781 tctgggaaaa caaaatgcca tgacaatagc agcaaataat gatacttccg taaggctcgc
     3841 cgtaaactgg ggagaggtga aattcctatg tgcttaccca aagcaattca aaaatttcat
     3901 aactactatt ttaggagatg attttataga atccagagtc tacctgaata aaaaacaaac
     3961 ctgtgcattt ctaggaatcg ataataatac tctagataag tggattcgta tatataattt
     4021 cccagtaata agagttggtg gtgtagttcg ctttgacgta gaagatatta aagactttat
     4081 gaatacttga aattttcttc tcgccgcata tactaaaatt agtgaaatat actgccttac
     4141 tttttttaag gagtgaggat tttgcctaca aagctttcaa atgggaaata taaaactaat
     4201 ctgagatatc caaaaaggtt taaagaaata acaggaattg catcagaaaa gtatcaaaaa
     4261 acatttccaa ataggcaatt ggcgattaaa gccgaaaatg atatgaagaa gaaaatagaa
     4321 aaagttttga gagaggagaa tgcaaactca ttagaattaa aaggaaaaat aacgtttaaa
     4381 aaattttacg aatctaaatg gcttcctaga tatgaattag gtcaaacaat tagatcaaac
     4441 aggcctccta gtgatataac cattagtaat acaaaagata tatttcggtt gcatatttta
     4501 cctatgtttg gtgaatacgc aatgaattat ctaaatatca atactgagat aatatcagat
     4561 gaattaacca aaaaatcgaa agaatatgca aatataaaaa tcataaaagg atatgttcgt
     4621 tccatgttcg atattgcaga aatactaaat tatatcgaat ttaatcgaac tactaaaatt
     4681 attcaaagta tcactgcacc taaaaaaaat gctttagaag aaaaaagaat acaagaaggt
     4741 aagcaagcac tatcatcaaa agaacttact aattggatag aagcagtaaa tgatgatttt
     4801 aataatcatt tacttacttt tcatgattac acacttttta tgattaccct gtatcttggt
     4861 gatagaaaaa gtgagactta cgctttgcaa tggaaatata ttgattttga aaaacaaacg
     4921 gtccgtctta aacatacttt agataaatat caaagaaaaa agtttacaaa aggtcgaaaa
     4981 gataccgtca ttcaagtacc agaagttgta atgacattgc taagtgaatg gaaatcggta
     5041 caagctgatc aattgttgaa attaaaaatt aagcaaacgt tagatcagta tctctttact
     5101 tatactaaac catctggaga agtaaattgt cctgttcatg ccgattacct aaactatcga
     5161 atcaattcaa ttaaaagaag gcatcctgac ttagttcatt tatctccaca caaactccgt
     5221 catacttatg caactatagc tagacaaggt ggagcagata tgaatcaaat ttcaaatgct
     5281 ttaacacact cagatatttc tacaacaaag atatatgtga acacaccaga tatagttgat
     5341 aaggctgttt ttgaagcttt tcagagagga ttgaataagt gtgattgata tgtgttctta
     5401 aatgtgttct taaaaacaaa agaagcactt tccaaataat tgaaaagtgc cagaaacgtt
     5461 gatatataaa ggatattaac gttttgagaa ttgtgaagct ttacgtgctt tcttaagacc
     5521 tggtttttta cgttcgacca tacgtgcgtc acgagtaagt aagccagcac gtttaagagc
     5581 taaacggaag tcaggatcaa cttctagcaa tgcacgagcg ataccatgac ggatagctcc
     5641 tgattgtcca gcgtatcctc cgccgtttac gttaacaaat acgtcatatg cgccttttgt
     5701 ttcagtaaca gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg gaatgtattc
     5761 ttcaacatct tttttattta cagtaatttt gccagttcct ggtactagac gtacgcgagc
     5821 aactgcgttt ttacgacggc ctgtgccgat atattgaact tgtgccaatg aaattccctc
     5881 ctattagatt aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat gtggatgttc
     5941 tgctccaccg tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt ttttaggaag
     6001 catacctttt acagaagttt cgatcaaacg acgagaattt ttagcacgca attcgccagc
     6061 agtgattgat ttcaatcctc ctgggtagtt gctgtgacgg taataaattt tgtcagtcgc
     6121 ttttttacct gttaatttca ctttgtctgc attgatgacg atgacgaaat cgccagtatc
     6181 caaatgaggt gtgaaagttg gtttattttt accacgtagt acagatgcaa caactgttga
     6241 aagacgtccc aatggaacat cagttgcgtc tactacatac catttacgtt ctacttcgcc
     6301 tggtttggcc atatatgttg tacgcacgtt attttcctcc aatttaagtt ctgatgtttg
     6361 acatacactt cgagtttccg gggctctttg tggggcaaac aataccattt aacatattac
     6421 ccgctaatct agtcaatgtc aacgtttttc tgcttgattg aaaaatttat atgaaacttt
     6481 acttcttttc tttcttttat ataaaagggt cgtttcctgt caaaatgaaa aacaaaaaga
     6541 ggctgtgaca aaagtcttgt cacaacccct tattttgaat aaacggtgtt ctcctgccaa
     6601 ctcctcggtc acaagtcaca atatccataa aacataagaa gctgtttttt aaaattgttt
     6661 cttgtgactc ggagctgaac ggcaggctta caacctcttg gtaaacggtg ttcaatcagc
     6721 tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttg acgtttgttt
     6781 ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa gttcaacaac
     6841 agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat ccagatacgt
     6901 ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc gccttcttct
     6961 ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc tgcaacaagt
     7021 gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg gatttttgtg
     7081 ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat caagcgttcg
     7141 gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat tgggtgcaat
     7201 gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac agacatttgg
     7261 cttagatctg caactaattg atttaatact tctttggttt gttggaattt catctttctt
     7321 gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa agtacaacaa
     7381 aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc aatcattttt
     7441 cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt gctaaatgct
     7501 tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca gtttcttgta
     7561 ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt tggatctttc
     7621 cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg acagtaggga
     7681 aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga gattgagcaa
     7741 acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct tttaaaatcc
     7801 gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt tctgtatgtt
     7861 gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt gctcgctcac
     7921 caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa gcgatagccg
     7981 aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg acttcctgta
     8041 aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct cctcccacta
     8101 cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg aactgaggct
     8161 gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg atcgtatcac
     8221 caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa acaaatgata
     8281 tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt acttgatagg
     8341 tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca agcacttctt
     8401 ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt ccgtagtcgc
     8461 tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca aaataagcag
     8521 tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc aatggtgtca
     8581 ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg gtatatttca
     8641 gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga gataacggtg
     8701 ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag acaaattgaa
     8761 tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag acaccttgaa
     8821 agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa gggtagcgaa
     8881 aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg gtttgtgtgg
     8941 ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta tgtatgtaca
     9001 aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc gtgttacttt
     9061 attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag cggattccgt
     9121 taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt tcgctttctt
     9181 ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc cgctattgca
     9241 ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt ctttttgcct
     9301 ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg ctttcggctt
     9361 tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg ctattctgtt
     9421 cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg caaagtgtgc
     9481 tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat ctcggagctg
     9541 gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag ttgcttcaga
     9601 tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt atctgtcata
     9661 aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg ctgattgtcc
     9721 ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct ctgatatata
     9781 gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca acctctccac
     9841 taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt ttacaacctc
     9901 tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg ccccaatgtc
     9961 gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg agcttgtttt
    10021 tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt ggttcgattt
    10081 ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt gaacgctctg
    10141 caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa tcaccgcgat
    10201 cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt tccaccaact
    10261 gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta tgccctgttg
    10321 tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta taaccggatg
    10381 tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa aagcatccgc
    10441 ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca taccttgatt
    10501 tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt atatacgtga
    10561 tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta aatcaggaat
    10621 cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat gcatgttacc
    10681 agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg catttggcgg
    10741 tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg cttttgatac
    10801 ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt ctaaaatgag
    10861 gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt tcaagtactc
    10921 aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa tcgcttgaag
    10981 atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga atttctgttc
    11041 catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc ctttcagttg
    11101 ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca gccacgcttc
    11161 gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg ctgctttgat
    11221 tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat gtttttagct
    11281 gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac agctgcagga
    11341 aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc tttaccaaca
    11401 cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat cccaactggc
    11461 gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac gaggtctgct
    11521 tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc agagactatt
    11581 ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact actgcccgat
    11641 gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg gtcagccact
    11701 gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc gccttctttt
    11761 ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat cagctcaata
    11821 ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag tgcttctact
    11881 ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc gatgccgcca
    11941 tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg ttgcaacaat
    12001 tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg ttggtttagt
    12061 gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg accgtctgaa
    12121 gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt ttgccgcttc
    12181 aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc tataggtagc
    12241 agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc ttgtgctgcc
    12301 tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg cattattttc
    12361 ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg acaaaaatag
    12421 gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa acaaatagca
    12481 acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa ggaagtcttg
    12541 gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat ataactgcta
    12601 gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc ggatctggtc
    12661 ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt tcttggatac
    12721 ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt gttttgtttt
    12781 catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt aataagtata
    12841 acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg ctacgggcat
    12901 aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa tcaataactg
    12961 gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc actggcaaat
    13021 ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca gcttgatatt
    13081 taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta ttgtcgatat
    13141 attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca tcgactttta
    13201 cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc tttgtttcta
    13261 aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca aggtctgcac
    13321 tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt ttgatattca
    13381 atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat gggaaaagtg
    13441 caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga tcataacggt
    13501 acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc aggataaaaa
    13561 tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc ttcgccccta
    13621 cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca gcgaagtcca
    13681 tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga tgccaaaaat
    13741 ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt ttctgggctt
    13801 gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc acgatgatct
    13861 tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg gtgatccggc
    13921 gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca atatcaattt
    13981 tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact cgcagttctt
    14041 tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc gttttcacat
    14101 ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc tgatggagat
    14161 tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga gcagtttcaa
    14221 tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg tatgcaagaa
    14281 ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat ctgatgacat
    14341 ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat acaccagctg
    14401 caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg ataccaaaaa
    14461 atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa tcaaactgtt
    14521 tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat ataaattttc
    14581 caccagattc tggatgacat atccttgcca gccatctgca atcatctgag tctggtcacc
    14641 gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa ctggcgtggt
    14701 cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg gatacagatt
    14761 agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga tattcaaagc
    14821 aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc caaaaattgc
    14881 atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat tttttttcgt
    14941 accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc cagtaatata
    15001 catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc cttgaagttc
    15061 tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat tcgcttctac
    15121 gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt cgtcagaaaa
    15181 acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct ctgccatctt
    15241 tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag cattagcttc
    15301 gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac tcacgatatc
    15361 gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa atcctgtacg
    15421 tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca attctaagac
    15481 atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc cagcgcctat
    15541 gattgctact ttcttcattt atttctccct cctttgctta tttacttatt gttattcttt
    15601 ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt tttcaagtat
    15661 tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca ttcatatacc
    15721 atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa atcaattgcg
    15781 ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac atactctttt
    15841 tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata aaaatcagcg
    15901 atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc cgctaacaaa
    15961 ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt acctgtttct
    16021 tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc tccttctttg
    16081 cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc tgctgggata
    16141 acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat ttgacgtaca
    16201 ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc gttacaagct
    16261 gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat attttctatt
    16321 aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa tctaaatttt
    16381 tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat tgttctcctc
    16441 ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca taacgcatgt
    16501 aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca caatccaagt
    16561 aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc catcttgggt
    16621 aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta ttgaaatgtg
    16681 cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca taagaaatga
    16741 tcccgccata gccgatccct cttaaagaag cttgtaatgc ggcagcactg ccactcgttt
    16801 caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa ccgacatctg
    16861 ctttcatagg atctagcgtg taatcggcac cgtatttttc tgctaattct ctacgctgcg
    16921 ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat tgaatcgcaa
    16981 tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga acatgtgctt
    17041 cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt ttccattgat
    17101 ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc agcgtttcac
    17161 gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc aaactgcccg
    17221 tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat accacaccgc
    17281 gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat tcttcatcga
    17341 taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga gtagcgaaaa
    17401 ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct ttgaatccgg
    17461 ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt cttttctttt
    17521 tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga taacgcatac
    17581 ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc ccaaatgtag
    17641 tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc ggaggtcgac
    17701 aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata aacgaagaca
    17761 gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg gatccggcta
    17821 agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa ataacttttt
    17881 agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag tgctgtattg
    17941 gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat caaaataccg
    18001 gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt gaagcgtagt
    18061 tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga ggctatcaag
    18121 gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc attgaaaata
    18181 tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt tgcaattgct
    18241 tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa attgacagct
    18301 aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt tttcatttca
    18361 agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac aatagaatcg
    18421 atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac aaataaacgc
    18481 ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag aacatccaga
    18541 tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt gtcacgatct
    18601 tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc gttttgtcga
    18661 ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc cagatagcaa
    18721 acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt gcttcgttga
    18781 aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta ttgaacatat
    18841 atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa atcagtgaac
    18901 gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct aatcctaaag
    18961 ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa gaacgataaa
    19021 attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg gcgtctgagt
    19081 taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt ggaatcagca
    19141 ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat tttaagtcag
    19201 aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat agctatctac
    19261 ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt gcgtcagcta
    19321 aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt ccgatcacac
    19381 ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt ccaaagactt
    19441 gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac ttgatgactt
    19501 cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga gcaccaccag
    19561 gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct gtgaatgctt
    19621 cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca gatactgtgc
    19681 catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct gagttccccg
    19741 cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct gttccattca
    19801 tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat tgggataagt
    19861 tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc tcgccttcat
    19921 catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc tcactcattg
    19981 ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct gtgaaatgtt
    20041 gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct tctgataatt
    20101 tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca atatagcttt
    20161 ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct tctccagctt
    20221 cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc atcactttag
    20281 caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct aacgtaacga
    20341 tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg tctggatttt
    20401 ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt tcgacagaag
    20461 agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt tcaccacaag
    20521 cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt ttcatttttt
    20581 tccacctctt attttattat tttcagccac atatattcat acggctggaa tatcaacttt
    20641 cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc tccttttggt
    20701 aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt ctcttccttc
    20761 gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat caaactattc
    20821 ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc accatatact
    20881 gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa aaatttttgc
    20941 cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt tcgacttccc
    21001 agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa tgaatgagca
    21061 gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa cgcatctaga
    21121 tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt gtagtttact
    21181 cgggtaccct ctttcttcag ctgttccact aattcaagga tcgttttttc cggcagaata
    21241 cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa aaaagttgtt
    21301 tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt attacccgaa
    21361 cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg tgcttcattc
    21421 tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc agtgatcaat
    21481 accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat tttatgcgtt
    21541 tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac agcgtccaag
    21601 cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat tttcaataaa
    21661 acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt ccatacgtgt
    21721 ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct tggtctagtc
    21781 acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta cgtatcattc
    21841 aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca gacaaagtcg
    21901 aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc caaacggtcg
    21961 ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg gaaaaatggc
    22021 aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt cttaaatgta
    22081 cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa gaaaatatct
    22141 tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac actgactttt
    22201 tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat cgcttgtaaa
    22261 agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg atttgatcat
    22321 attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata tacacaaaaa
    22381 tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat atggtctctc
    22441 atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa tgtctattgt
    22501 actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt gaaacctact
    22561 atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag agtattaaat
    22621 aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat caaagaattg
    22681 aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga cgcgattcta
    22741 gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc agatgttatt
    22801 caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca aacggcaaaa
    22861 acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat gatccgagga
    22921 attattattt taagctctac gatggatgaa acacttcttt ttgaattgga tcaatacaat
    22981 attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta ctcagtggac
    23041 acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa tggacaccgt
    23101 catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg tctcaatggt
    23161 taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat tatagacggc
    23221 ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct acgagaaaga
    23281 ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa agtggcagaa
    23341 caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga tcccttattg
    23401 tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca gttaggaatc
    23461 aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga aaaaagaatc
    23521 attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat gtagaacaga
    23581 attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt ttcaatcact
    23641 catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc atctgtttta
    23701 gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg gaagaaaaaa
    23761 ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac acatttaccc
    23821 ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag aataacagac
    23881 atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca aaatattaaa
    23941 aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc tatctttact
    24001 tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg atacgaaccg
    24061 atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt agacaaacca
    24121 aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct attttgtttg
    24181 attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac tgtagacatt
    24241 ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca taccagtacc
    24301 agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag gatctttctt
    24361 accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg cagcagataa
    24421 gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc gagaagttgc
    24481 aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg cgatgtctaa
    24541 taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt tacgcagcat
    24601 ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca tacggtaaac
    24661 acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa cttgcaacaa
    24721 ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca tatcgccttc
    24781 tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg tttttccttt
    24841 gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga catcccctgt
    24901 tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt cttggacacg
    24961 aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg tacgcatcgt
    25021 caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg cttctccgac
    25081 ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga caccatgttt
    25141 tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat caacgatttg
    25201 ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat ttgtttcagg
    25261 atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg attcgatgat
    25321 ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc aatcttcttc
    25381 acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc
    25441 agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga taaacatttc
    25501 caagacggat agtccttcac ggaagttcga aatgattggc aattccatga ttcgaccatt
    25561 aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa tgttaccacg
    25621 agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt ccatcagttt
    25681 ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat aacgttcgtc
    25741 atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt
    25801 ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta ctgtgatacc
    25861 ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct tagaagtttc
    25921 tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt tcttcttgaa
    25981 cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag cttcaacaaa
    26041 gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca gataagggaa
    26101 ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca tcgtacgttc
    26161 tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac cgatacgtga
    26221 atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat ctctgaagac
    26281 cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac ctagaaccat
    26341 atcttgggaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt tttgagcagc
    26401 taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt ggacagccat
    26461 ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca aacggattgc
    26521 gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc
    26581 ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc atacttcgtc
    26641 ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac
    26701 aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg gaagtccaca
    26761 ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt agtcgacacg
    26821 tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat gagaaagaga
    26881 tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa
    26941 tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc caggtgcatt
    27001 caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt aaagatcatt
    27061 caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta aatctggtgg
    27121 aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag cgcggaatgc
    27181 ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa
    27241 ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta attgtttaat
    27301 cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt tttcgcgata
    27361 ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt cgatgactac
    27421 atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta atacaagacc
    27481 catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg ctaattcaat
    27541 atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc atcgatcaca
    27601 aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc agtcttttgt
    27661 aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg tacgatagtt
    27721 aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt cgggagaagc
    27781 caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt ccctccattt
    27841 catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc atagaccgga
    27901 ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt tttcatcttc
    27961 cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg caaattttgt
    28021 caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc
    28081 tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt ctttcaccaa
    28141 tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga cgattgcttc
    28201 gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt
    28261 ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac gttgtccacc
    28321 aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc cgattgaacg
    28381 tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca caccgacaga
    28441 cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt tcgcatcgct
    28501 ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac catcaaatac
    28561 tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta attccaatac
    28621 ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat cgattggtgt
    28681 tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa cccctttatt
    28741 tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa cgatataaac
    28801 acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag taaagatttt
    28861 cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat cgcggacttc
    28921 acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata attctgttac
    28981 cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg caccaatacg
    29041 gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg
    29101 tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt cttcaatatg
    29161 gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg catcttcata
    29221 gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag ccatttcgcc
    29281 ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct cacccaaatg
    29341 aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt ttgttacatc
    29401 gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat caacgtattc
    29461 aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg atttgtattc
    29521 cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt gacgctgcat
    29581 gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa tacatgctgt
    29641 cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat caatagatac
    29701 ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg ttccatcttc
    29761 attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag ctgtcaagta
    29821 gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag gtgtttcgat
    29881 aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc cgatgtttgg
    29941 gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa cgtcacgaac
    30001 ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa gacgacgttt
    30061 gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact gagaagaacc
    30121 aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt gaggtgtcaa
    30181 tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac gtgctagccc
    30241 gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat ttcctaagtg
    30301 gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc tcattgaagc
    30361 aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt ttccgatcac
    30421 gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga cagtcattgg
    30481 atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc cattatcaat
    30541 gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt caacgatgat
    30601 ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata gacgtgtttt
    30661 caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt aacgttttgg
    30721 atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg gttcgcctgg
    30781 acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt
    30841 atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat
    30901 cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt
    30961 acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta gccaagctcc
    31021 acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga cttttccatg
    31081 gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg ccccgttgat
    31141 gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt cttgtgcttt
    31201 gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt
    31261 tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt cataatctac
    31321 aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt caaacatttc
    31381 tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga tcaaatttgg
    31441 taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt
    31501 tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa gactacccga
    31561 tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca ttttttgcgg
    31621 cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact tcgtgagctt
    31681 ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct gccttttctt
    31741 catcagatag tttttgcaac ttcatatact acacttattt tctataaaag tcaacacttc
    31801 ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa
    31861 ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta gaaaattcat
    31921 tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca caactgaata
    31981 acttttactt tagctacttt agataggttt gtattcatca tgatggatac aaacgttaaa
    32041 cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac
    32101 aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca ttctactttt
    32161 tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg attttttgtt
    32221 tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat taggcgctgc
    32281 cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg catcagatgg
    32341 acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg ctgcctataa
    32401 aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc taacaatcga
    32461 ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa
    32521 agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt tattaaatga
    32581 aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag cccatttagt
    32641 cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg tgccaatcga
    32701 aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg gcatcatgta
    32761 taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag aattgaaaaa
    32821 agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc aacaagtgac
    32881 gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca cgttcgcaag
    32941 tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc
    33001 agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg aaagcttggg
    33061 taaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa gtgaacttcc
    33121 tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca aagcattagg
    33181 catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct catgacaaat
    33241 ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct acttgcgatt
    33301 ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga aggaagcttt
    33361 cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc attgacagct
    33421 tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact gcttcacaca
    33481 aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt atattctgtc
    33541 acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac gatctttcat
    33601 tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt
    33661 gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg acttgctatt
    33721 cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac agataatatg
    33781 aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc cttgcttgcg
    33841 caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat tggacttgct
    33901 tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg tctcttgact
    33961 gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga actgaatgtt
    34021 gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct ttctttcccg
    34081 cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg
    34141 acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc aacgaaaatc
    34201 atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg atcatcggtg
    34261 gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt tctgtcgatc
    34321 agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga
    34381 aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt ttaacggtag
    34441 aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca
    34501 aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg gggttggaaa
    34561 atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct attacgttat
    34621 tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact gcagcgcttg
    34681 accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact gagcagaatc
    34741 tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg agtcgactga
    34801 tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa cagcagctca
    34861 cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta aaagacgatc
    34921 aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg cccaccctct
    34981 tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt ttcttcgaaa
    35041 actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt gatgccatga
    35101 caacgaaaga caaggtactt caattattga aaaagacacc agattttctt tccggagaaa
    35161 agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc aaagaattgg
    35221 aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg ccctctgacg
    35281 tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat ttgaaagtac
    35341 aaacgatgtt gacttctgaa tctacgatga aagatgtcaa actggcagca gctcaaggag
    35401 attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga cgttttggac
    35461 ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc catccagaca
    35521 aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt gtgaaagcca
    35581 ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc tacatggatg
    35641 gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc ggcaggatca
    35701 gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat tttccagctg
    35761 atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc acaaggaatc
    35821 agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca gaaaaagcgt
    35881 tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc aatcaaaaag
    35941 gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg atcgtccaaa
    36001 caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct atccgtacgc
    36061 aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt ttttctgaat
    36121 tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct ctagaaaaag
    36181 gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca atgcaaaaaa
    36241 caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct ttttcaatct
    36301 tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta ttttgccgta
    36361 agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg catagatcac
    36421 ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc ctcgtgtgac
    36481 tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca gccttcttaa
    36541 gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt ctatttgatt
    36601 agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt tatctgctat
    36661 cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag ttcctgctaa
    36721 atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag tcattgattt
    36781 gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag agacgccttc
    36841 aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca tatctaaata
    36901 atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt ctaggatacc
    36961 tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca tcatcaatag
    37021 tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa gcaacaatat
    37081 gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca gtttaagact
    37141 aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa aaatgaaaaa
    37201 aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt atggtgtggc
    37261 cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg atctaactgg
    37321 taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa ataagacaac
    37381 tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa aaaataattt
    37441 tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac aaaaaactga
    37501 ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata aatttgtcgt
    37561 tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg gatccaggct
    37621 tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat atattgattt
    37681 cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga tcactccagc
    37741 taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg atttaactga
    37801 tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa cgatgaatac
    37861 gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca tcaacagttc
    37921 ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc atgaaacaga
    37981 agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg atttgacaat
    38041 cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca ttactcaaaa
    38101 ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca atcgttggga
    38161 tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa gcgacagtac
    38221 agatacatct accacaagtg atagctcaga ttcatcaact tcaagcgaca gtacagatac
    38281 tacctcta
//
LOCUS       DAZKMB010000013        37632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000013 DAZKMB010000000
VERSION     DAZKMB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.013"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            114..2279
                     /gene="acm"
                     /locus_tag="ACG8V3_000624"
     CDS             114..2279
                     /gene="acm"
                     /locus_tag="ACG8V3_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0299188.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2551..3036)
                     /locus_tag="ACG8V3_000625"
     CDS             complement(2551..3036)
                     /locus_tag="ACG8V3_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0299189.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3126..3471
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3516..4157
                     /locus_tag="ACG8V3_000626"
     CDS             3516..4157
                     /locus_tag="ACG8V3_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0299190.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4176..4811
                     /locus_tag="ACG8V3_000627"
     CDS             4176..4811
                     /locus_tag="ACG8V3_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299191.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4829..5644
                     /locus_tag="ACG8V3_000628"
     CDS             4829..5644
                     /locus_tag="ACG8V3_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0299192.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5963..7399
                     /locus_tag="ACG8V3_000629"
     CDS             5963..7399
                     /locus_tag="ACG8V3_000629"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0299193.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7588..10035
                     /locus_tag="ACG8V3_000630"
     CDS             7588..10035
                     /locus_tag="ACG8V3_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0299194.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10265..11332
                     /gene="rlmN"
                     /locus_tag="ACG8V3_000631"
     CDS             10265..11332
                     /gene="rlmN"
                     /locus_tag="ACG8V3_000631"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0299195.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11322..11528
                     /locus_tag="ACG8V3_000632"
     CDS             11322..11528
                     /locus_tag="ACG8V3_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299196.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11525..12628)
                     /locus_tag="ACG8V3_000633"
     CDS             complement(11525..12628)
                     /locus_tag="ACG8V3_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0299197.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13181..13729
                     /gene="aac(6')"
                     /locus_tag="ACG8V3_000634"
     CDS             13181..13729
                     /gene="aac(6')"
                     /locus_tag="ACG8V3_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0299198.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(13977..14096)
                     /locus_tag="ACG8V3_000635"
     CDS             complement(13977..14096)
                     /locus_tag="ACG8V3_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0299199.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            14275..15936
                     /locus_tag="ACG8V3_000636"
     CDS             14275..15936
                     /locus_tag="ACG8V3_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0299200.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(16006..16353)
                     /locus_tag="ACG8V3_000637"
     CDS             complement(16006..16353)
                     /locus_tag="ACG8V3_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0299201.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16437..17378
                     /gene="opp3b"
                     /locus_tag="ACG8V3_000638"
     CDS             16437..17378
                     /gene="opp3b"
                     /locus_tag="ACG8V3_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0299202.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17379..18422
                     /locus_tag="ACG8V3_000639"
     CDS             17379..18422
                     /locus_tag="ACG8V3_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0299203.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18428..19498
                     /locus_tag="ACG8V3_000640"
     CDS             18428..19498
                     /locus_tag="ACG8V3_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299204.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19488..20441
                     /locus_tag="ACG8V3_000641"
     CDS             19488..20441
                     /locus_tag="ACG8V3_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299205.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20453..21463
                     /locus_tag="ACG8V3_000642"
     CDS             20453..21463
                     /locus_tag="ACG8V3_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0299206.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21748..22569)
                     /locus_tag="ACG8V3_000643"
     CDS             complement(21748..22569)
                     /locus_tag="ACG8V3_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0299207.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22666..22944)
                     /locus_tag="ACG8V3_000644"
     CDS             complement(22666..22944)
                     /locus_tag="ACG8V3_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299208.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23212..25221
                     /gene="metG"
                     /locus_tag="ACG8V3_000645"
     CDS             23212..25221
                     /gene="metG"
                     /locus_tag="ACG8V3_000645"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0299209.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25700..26287)
                     /locus_tag="ACG8V3_000646"
     CDS             complement(25700..26287)
                     /locus_tag="ACG8V3_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299210.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26462..27784
                     /locus_tag="ACG8V3_000647"
     CDS             26462..27784
                     /locus_tag="ACG8V3_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0299211.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            27939..28715
                     /locus_tag="ACG8V3_000648"
     CDS             27939..28715
                     /locus_tag="ACG8V3_000648"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0299212.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28708..29277
                     /gene="rnmV"
                     /locus_tag="ACG8V3_000649"
     CDS             28708..29277
                     /gene="rnmV"
                     /locus_tag="ACG8V3_000649"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0299213.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29270..30154
                     /gene="rsmA"
                     /locus_tag="ACG8V3_000650"
     CDS             29270..30154
                     /gene="rsmA"
                     /locus_tag="ACG8V3_000650"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0299214.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30605..31234
                     /locus_tag="ACG8V3_000651"
     CDS             30605..31234
                     /locus_tag="ACG8V3_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299215.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31358..32467)
                     /locus_tag="ACG8V3_000652"
     CDS             complement(31358..32467)
                     /locus_tag="ACG8V3_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299216.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32676..33098
                     /gene="mgsA"
                     /locus_tag="ACG8V3_000653"
     CDS             32676..33098
                     /gene="mgsA"
                     /locus_tag="ACG8V3_000653"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0299217.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      33285..33383
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            33421..33957
                     /locus_tag="ACG8V3_000654"
     CDS             33421..33957
                     /locus_tag="ACG8V3_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0299218.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(33997..35760)
                     /locus_tag="ACG8V3_000655"
     CDS             complement(33997..35760)
                     /locus_tag="ACG8V3_000655"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299219.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35763..37478)
                     /locus_tag="ACG8V3_000656"
     CDS             complement(35763..37478)
                     /locus_tag="ACG8V3_000656"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299220.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT        12409 a         6636 c         7762 g        10825 t
ORIGIN      
        1 atcaattttt tagttaaaag attggaaatg agtgggggag gaattgaaga aaggattttt
       61 gagagatgat atagtaggtt ttatttttag ttaaaatagg ggagatagaa aaaatgaaaa
      121 agtgtagaaa tattatttta tcaatgttat ttattattac taatataaca tcactgattc
      181 ctgttcatgt ctatgcggat gcaggcagag atatcagcag taatgtcact tcgttgaccg
      241 tggacccaac gaatattaca gatggaggaa acataaaagt aaaattctcg tttgacgaaa
      301 aaaaacaaaa tatccaacca ggtgattatc tttggatcaa ttggccaagt gaaggaaata
      361 ttcgcggtga aggattccaa aaagaaatcc cattaatgat tgaaaataaa aatgttggaa
      421 ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt aaaaacctag
      481 actcagtaga aggttggggg caatttgaga tacaggccag aaacgtaacc gataccggtg
      541 aagaaaatac gggaccattc accgtgacga gcggtgataa aacagctact gtaaatgtga
      601 caaagccagc ttctggttct tcttctagcg ttttttatta taaaacagga gacatgctac
      661 ctgaagatac taagcacatt cgatggtttt tgaatattaa caataacggc acttatgttg
      721 aacaaccagt taaaatcagt gatgaaatcc aaagtggaca aagattagat ccaagtacgt
      781 ttgaaattaa tcaaatacac ttaggtgaac agaaggttta tagaggagaa gagggaatcc
      841 aacaattttt acaagatttc cccagcgcta catttaattt ttcagtaact gataattaca
      901 ttgaaataac tataccaaaa aactttgtca atcttaggaa aataatggtg agttataaga
      961 cgatcataga aaacccagaa caaataaatt ttgagaatca ttcggaagct tggtttaaag
     1021 aatttaataa acctgctgtt gatggagaaa gtttcaatca tacagttaaa aatatttcag
     1081 cttctggtgg agtcaacggt acagttagag gcgaattgaa gattttcaaa tatatcaatg
     1141 acacagagat tggtatccca aatgtgactt tcgaattaag acgagcagat gaacagccta
     1201 ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca gggagaagct agtatcaaag
     1261 gcctgcaagt aggagactat gttgtaaaag aaaaagaagc acccaattgg attgattttg
     1321 atccactaag ttcaaatgaa ctcaaattct ccataaacga aaatgataca gagggagtca
     1381 gtttacctat ttataacaag aaaaaggtta cgaacatcac agcaactaaa atttggaacg
     1441 gtggaaccac tccaagaccg agtatttatt ttaaattatt cagagcaagt acaaacaatt
     1501 gggaaccagt tcctgatgca gaaacaaaac gattagacaa tggaataact tcagtgactt
     1561 gggaagatat ccaacagtat gatgatagtg gaaacgaata tacgtttaaa gtgcaagaag
     1621 tagatcaaaa cggaaatgat tatgtccctt caggttatca aaaaattgaa aacgggctag
     1681 tcgttacaaa tgagaataaa gaagtcattt cagtctcagg acaaaaaaca tgggaagaca
     1741 atgagaacca agacggcaaa cgaccgacga cgatcacagt aaatctatta gccgacggtc
     1801 aaccgatcca acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt ttcacaaatc
     1861 tgccaaaata cagagacggg caagaaatca tctatactgt aacggaagac aacgtgcccg
     1921 aatacagtac gacgattgac gggtataaca tcaaaaacag ttatacaccg gaaaaaacaa
     1981 atatatctat accaggtagc catataacac cgtggaaaaa ttttccagag aagacagaga
     2041 gagagaaaat tagaaatcta ttctctcaaa aattgcaaat atttgctcat acaggagaat
     2101 ccagcgaaca aagaagcaat tatattagta agagagctgt acctaaacag ataacaaata
     2161 aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc tagttttgct gtaatcattg
     2221 gtttattgat agtaactatc tgtgctggga tattcttaca aaaatataaa aaagtttagt
     2281 tagaatataa tattttttgt taaatatgac tgaaatcaaa agaataaaat tccgatttat
     2341 aatcgaaaaa agaagcattt ttatatatgc ttcttttttc gtagaatcaa taaatatgga
     2401 tttggctaac tatggaagaa tactcgtatt agttttcagc gttcatacta gttgttacat
     2461 tgtttgattg gccaaaagaa tttctgatat attttgtgct aaataaagaa aaagacgctt
     2521 gaattgggaa ataagaaaac tactctatca ttacaaattt tcataaactg gcagatgttc
     2581 taagtaaaca agtatctttt caaagtcgga aaaatccata atcaacgtag tggtattgat
     2641 atttggatga aagcctacgg tatcttgacg atcaatagca gcatcgatca cgacttggat
     2701 ttttttctct ttgtcgtgca tcaatgctaa tggagtcact gtacctgctg gaacgcctag
     2761 gaggtcagga agctgttctt cagaaacaaa agataagcgc ttttctttta attgttcagc
     2821 tagttgtttt aaatcggcgt gcttttcatc aggcaagatg acaagataga tttgttttcc
     2881 ttttcttgat ttcaataata gattttttac ttgcggacca ggtagggaaa aagggacatt
     2941 tttgactgac gtaatcgcag gatgatcgac tttttgaaaa gagataccca gtttttttag
     3001 tagttcataa gcttcttgtt cggttgcacg actcatcgcg catcaccctc ctttttttat
     3061 tagtgtatca taaaaaagag ttgacaaatg aaaaataatc atgtaaatta cacgtaaagt
     3121 taaaacaaat gacatcgaaa agaagagtag ccgttaggtg tttttagaga gtctctggtt
     3181 ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg agtcgatttt ctgaagcgta
     3241 tagtaggaaa atccggggag cccgttacag ctgcggtcgt tgctagatga ccagataagg
     3301 ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc acgtgattaa cgtcctttga
     3361 tcttcggatc aaaggacttt ttgttttcat ctgaacggca acgactgtgt gaggtttttt
     3421 ctgcaaggaa aaaataaaca gaggtggaac cacgtataat acgtcctctt ggcatttgcc
     3481 aagaggattt ttttatttta aaggaggaaa agaagatgga gtatttgatt gaagttatgc
     3541 cgcaattatt agtaggagca ctcacaacat tgaaattatt tttatttacc ttgattggct
     3601 cattaccatt agggattgtt ttagcattag gattgtcttt gcattttaag ccgctacgct
     3661 acttgcttca aacatatgta tgggttatgc ggggaacacc gctgttgttg caactgattt
     3721 ttatttttta tgggttgccg acgattggga tcgtttttga tcgtttcgaa gcagcactca
     3781 ttgcctttat cttaaactat gcagcttatt ttgcagagat ctttcgtgga gggatacaat
     3841 cgattcctca aggtcaatat gaagcagcgc aagttttgca attaagtccc tttcaaacgg
     3901 tgacgaaaat cgtcatccca caagtgacaa agattgtttt accttctgta gggaatgaag
     3961 ttatcaactt ggtcaaagat tcttccttga tctatatatt aggtttaggt gacgtaatgc
     4021 gtgctgggaa aatcgcgatg gaacgcgacg cgactttact gcctttgcta ggcgtggcaa
     4081 tcatctatct tacattgact ggtattctaa caatcataat gaaacaagtt gaaggaaaat
     4141 taaattatta ccgctaactg ataggaggaa gaaatatgat tgaattgaag aaagtgaaca
     4201 aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct aatcattcca gatgggaaaa
     4261 cattagctat cgtaggtcct tccggtggcg gtaaaaccac gcttttacgt atcttggctg
     4321 gcttagaaag ttctgacagc ggagaatttt atttagacgg tgtaccgttc aacccattcg
     4381 agcttaaaat gaaagaacaa gttgtaggag tcgtattcca agacttccag ctatttccac
     4441 atctgaatat ttttgaaaac attaccttag caccaaaaat ggtattgaaa cagacaaaag
     4501 aagaatacac caaaaaagca gaagaattag cggcttcatt agggattagt gatttattga
     4561 atatctatcc ttatcagctt tctggcggac aaaaacagcg ggtagctatt gccagagcat
     4621 tggctatgaa tccacgggta ttggcttatg atgaaccaac aagtgcttta gatcctgaat
     4681 tacgccagca agtcgagcgt ttgatcctat cattgaaaga agatggggta acccaaatta
     4741 ttgtcaccca tgatttagta tttgcagaaa atgtcggtga tcaattacta aaagtttccc
     4801 caattcatta agaaaggaga agatagagat gaaaaaaacc agttttctat tcgctttgat
     4861 cgcaggtctt cttctttttg caggctgttc gaacaaaaaa acttcagcag ataattgggc
     4921 gactatcaac gaagagaaaa aaatcgttat aggtttagac gatacatttg ttcctatggg
     4981 atttagagat aaagacggga agctcacagg atttgatatt gatttagcca aagcagtctt
     5041 tgaacaatat gggattacgg tagactttca gcccattgac tggtcaatga aggaatttga
     5101 actgaacaat ggaacgatcg atttgatatg gaacggctat tccaaaacgc cggcaagaga
     5161 aaagaaagtg caatttacaa aaccttatat ggaaaacgat caagtgctga tcacacctaa
     5221 agaatcagga atcacaaact ttggacaaat gaataataag agattaggtg cgcaaaatgg
     5281 gtcatctggt tatgatgtat ttacgaaaca accagaagta ttaaaagaga tagtcaaaaa
     5341 ccaagatgct gttttgtatg atagttttaa tgaagcattg atcgatctga aaagcagacg
     5401 ggtcgatggt ctgctgatgg ataaagtata cgcagactat tatttgaaac aaagaaacga
     5461 actagaagac ttcaacataa caaaaggttc ttatgagagt gaggattttg cagttggtgc
     5521 acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa gccctagatc aactagagaa
     5581 aacaggtgaa ttccaaaaaa tatccgataa atggtttggc gaagatgtta cgccgagaaa
     5641 ttaacaaata ggggactgtg acaaaagacg tgtcacagtc cctttttccg aataaacagt
     5701 gttctcctgc caactccttg tgactcggag ctgaatggca ggctcacaac ctcttggtaa
     5761 acggtgttca aaagccagtt tttcggtatt aagccaaatt tatgcaaaaa tgtagaaagc
     5821 cattttcaca aaatttttct tactactcga aactaagcga ctttttcaca acctccttta
     5881 cataatttta ttttgttgtg gaaggggttt cgtgttattc taaaagagga acgattgaag
     5941 gttataggaa aggaagagat gaatggaaca caaagaactt acctcatttc caaaagattt
     6001 tctgtggggc tctgcttctg cagcgtatca agtagaagga gcttggcaag aagatgggaa
     6061 aggagaatca gtctgggatc gatttgtacg gatacctgga aagacattta aaggaacgaa
     6121 cggtgatttg gcagtagacc actatcatcg ttataaagaa gatattgctt taatgaaaga
     6181 acaaggtctg aaagcttacc gcttctctgt agcttggaca cggatttttc ctaatggacg
     6241 aggagaagtc aatcaagctg ggctcgcttt ttatgagaga ttgatcgacg aattgattga
     6301 aaacgatatt gaacctatgt tgactttata tcattgggat cttccacaag cattgcaaga
     6361 tgaatataat ggttgggaat cacgtcagat cattgaagat ttcactcatt atgcagaaac
     6421 attatttgag gcattccgcg ggaaagtcca ttactggatt agtttgaatg aacaaaatat
     6481 cttcacttca ttaggttatt tgttagcagc acatccgcca ggtgtgacag acccgaaacg
     6541 aatgtatgaa gttaaccata ttgctaattt agcaaatgca tctgtcatca ataaatttca
     6601 cgagttagaa atgcctggaa aaatcggacc aagctttgcg tatacgccaa attatccaat
     6661 tgacagtaat cctgaaaatg ttctggctgc tgaaaatgca gaagacctga tggccaatta
     6721 ttggatggat gtctatatgt ggggaaaata tccgattgct gccatgaggt tcttagaaga
     6781 aaaaggatgg gcaccaacga ttgaagctgg cgatgcagaa ttgctagaat ctgctaaacc
     6841 agattttctt gggatcaatt actatcaaac ggcgacgaat gcattcaatc cattaaacgg
     6901 agttggtgct ggaaaaatga atacgacagg aaaaaaaggt tcatcagaag aaactggagt
     6961 tcctggaatg tataaaaaag ttgagaatcc atttgtcgaa cgaacaaatt gggactggga
     7021 gattgaccca gaaggattaa ggatcggact gcgtcgtatc acaagccgtt accgcatgcc
     7081 ggtactgatt acggaaaatg gcttaggaga gtatgacaag ttgacagaag ataaacaaat
     7141 ccatgacgac tatcgaatca attacttaca aagccacgtc aaagccatca aagaagcaat
     7201 ttcggatgga gcagaagtcc taggctattg cacctggtct tatacagatt tgcttagctg
     7261 gctgaacggt tatcaaaagc gatatggatt tgtatatgtt gatcaagacg aaacacaaga
     7321 aggatctttg gaacgatata agaaagacag ttattactgg taccaaaaac tgattgaaga
     7381 aaatggagag aacctttagt tcacatactt aaaacaagaa ggtacggctg gatatggccg
     7441 tgccttttct tgataaaatc atttagaaat taagaaaaac tgtaaaataa gctgaaaaat
     7501 gaaagaaagc gtcagttctt tcaaacaaaa ctttagttcc cttacttttt tttgtataat
     7561 gaaacttgat gaaatgaggg ataataaatg gcacaaaaga aacggcctac taaaaaaagg
     7621 aaaaagacga aaaaaacgaa aaagcaaaaa cagcagcagg aacaaatgct gacgattggt
     7681 gttgggtttg ccttgatttt atttgctgtc ttcggctttt taaaattagg ttttttagga
     7741 atattgattg caaacggctt tcggatcatt gcaggaaata cttaccaaat cctctgtctt
     7801 ttattagccg tattaggatt ttggattgtc attaagaata cagagttttc aattggtaaa
     7861 aaccgacgct ggtttggcgg catactcttt tatttcggta tcttattatt gttacatgcc
     7921 catttatttg gcaaactcca tactggggaa ccaaatatca tggggacgac atgggatttg
     7981 ttagccagtg atataaaaca aagtcaagta gacaataacg ttggtggtgg gatgattgga
     8041 gcaatccttt atcattttac ttacttcttg attgcacagc ctggaagtta tttagcagca
     8101 gtgctattac tggcaggagg agcattcctg ttcagtaatc tggaaggcta tcagctgtta
     8161 aatggtatcc aatcaattgg agaacgagta caggaattat tggaaggtga tcctgccaag
     8221 caagctcgaa agcaagcggc aaaggaagag agaatgaaac agcgcgcaga agcgaaagaa
     8281 gcaaggcgct tagctgcaaa agaagctgca gaaaaagaag ctgcagaata tgagaagaaa
     8341 aaagcggtga gccagcaaag ggaaaatact gctgatgaat ggcaggaacc agaaaataca
     8401 gagccggagc agctaagttt tgttccaatc gacagttttc aagaaaatat ccatcctgcg
     8461 aatctagaga aaccagtacc agatacacca aaacagacca atacagcaga aggtttttca
     8521 gatgagttgc cagaggatga cggtacctca ttagaatttg agattgaagc agaacaggaa
     8581 aatcaagact atgaattacc gacagttgat ttgcttgatt ctataccaac tgtggatcaa
     8641 agtgatgagt ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac tttccaaagt
     8701 ttcggtgtcg atgccaaggt cgtcaaagcc agcttaggac catccgtcac aaaattcgaa
     8761 gtccagccgg ctgtaggggt aaaagtaagt aaaattgtga acttaacaga tgatatcgcg
     8821 cttgctttag cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa atcactgatt
     8881 ggtattgaag taccaaatgg aaagatcagt atggtttcct ttagagaaat catcgaagca
     8941 caaccaaacc acccagataa attactagaa gtacctttag gacgtgatgt ttctggacgg
     9001 gtacaaacgg ctgatctttc gaagatgcct cacttactcg tggctggttc gactggtagt
     9061 gggaagtcag tagcaattaa tgggattatc acaagtatat taatgcgtgc taagccccat
     9121 gaagtaaaac tgatgatgat cgatccgaaa atggttgaat tgaatatgta caacggcata
     9181 cctcatctgt tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc cttacaaaaa
     9241 gtagtacaag aaatggaaga acgatatgaa aaattcgcgg caaccggagt aagaaatatt
     9301 tctggttaca atgaatttgt tcaacagaaa aacttggaaa acggaacgaa acatccaacc
     9361 ttaccattca tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt agccagcaat
     9421 gaagttgaag atgcgattat tcgtttagcg caaatggctc gggcagcggg gattcatatg
     9481 atcctagcta ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa agcaaatgta
     9541 ccttccagaa tggcatttgc ggtatcaagc ggtacagatt ctcggacgat cattgattct
     9601 aatggggcag aaaagctctt agggcgagga gatatgctat tcttgccaat gggtgagaat
     9661 aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg aagtagaacg ggtcgttcaa
     9721 tttgttacag atcagcaaga agctcattat gaagagaaaa tgatgccgac agatgaagtt
     9781 gaaacgacag gtgctccaga acagccacaa gatgagctgt ttgaagaagc aaaagcactt
     9841 gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa gaagatttcg gatcggttac
     9901 aaccgagcag cacgtttagt agatgaactg gaagctcatg gagttgttgg cccgtcagaa
     9961 ggaagcaagc ctcggaaagt attcattgag caggaagaaa catcagaaac gttgccggac
    10021 gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc aataaaaaga
    10081 caaggcatga tgataccaaa agaaaaaatg gtttttttcc atcttttcta atacggtatg
    10141 atgtcttgtc tttttagttt ttgtgagatt aaagttctat tcgatttatt tgtgaagtat
    10201 tgcattaagg aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa agagggaaaa
    10261 tactttggaa aaacagtcca tttatggctt gacgaatgaa gaattaatca attggtttat
    10321 agaaaacggc gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt atcaaaaacg
    10381 tgtaagtaat ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa aattatcgga
    10441 gcattttatc atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg atggtacagt
    10501 aaaatatttg tttgaactcc ctgataaaaa catgattgag actgtactga tgagacaaga
    10561 atatggcttg tcagtttgtg tgacgacaca agtaggatgt aatatcggct gtacgttttg
    10621 tgcaagcggg ctgctcaaaa agaaccgtga tctgactgct ggagaaattg ttgcacagat
    10681 catgatggtc caacattatt ttgatgagcg caatcttggc gaacgcgttt cacatgtcgt
    10741 tgtaatgggg atcggtgagc catttgataa ttacgacaat gtaatggact ttttgcatat
    10801 catcaatgat gccaaaggat tggcaattgg tgctcggcac attacagttt ccactagtgg
    10861 attagcacac aaaatcaagg aattcgctaa taacggcttg caagtcaatc tggctatttc
    10921 cttgcacgcg ccgaataatg aggtgcgaac atcgatcatg cggatcaacc gaagtttccc
    10981 aattgagaaa ttgatggaag ctgtggatga gtatttagag aaaacaaacc gtcgaatcac
    11041 gtttgaatat atcatgttga atcaagtaaa tgatagacca gagcatgcgc aacaattagc
    11101 agatctatta aaagataaga aaaaattagc atatgtcaac ttgattccat ataatccagt
    11161 cagtgaacat gatcaatatt ctcgcagtcc aaaagctgat gtattgagat tttatgatgt
    11221 attgaagaaa aacggcgtaa actgtgtgat acgtaaagaa catggtacag atattgatgc
    11281 agcttgcgga caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat aatcgtatcc
    11341 tactcgtttt ttcaattacc ataaaaggag cagttttctt aattaaggaa ctgttcaact
    11401 taaataagag aaaagattct atagcgaaag ctataaagct ttttttgaca agaaaaaccc
    11461 tcatcaagca aagaaatgtc ctcaatttga tgacagcctg cttgatgggg gttttagcaa
    11521 ttatttaact gattttctta tatccttgct ggctttcttg tgtagcatga taagtttcta
    11581 cgtcaagaat ggcctgttta aatttttctc gggtgatttc ataaggcaca tgtttaatat
    11641 catttgttgc catggcttta tctaagattg cttctaaatc ggtcgtgaca ttgagattca
    11701 tgtcggttaa cgatactgga agttggttct ttttcataaa cacatagatt ttatcaaaca
    11761 aatcgaactg ctgatccatc atcaataagg tcagtacacc ataactgaca gatgctccgt
    11821 gaaggtatcc ctccattttt tctagaacag tcgtaccgta atagaatgag tgtgccatag
    11881 cagaattata gttattgtta actaaaacag aaaccaatcc ggtagtaaca atgatatccg
    11941 aaacgacttg ttcaattgca tatgagggac gatcgtcggc actatctttc aatgcttgcg
    12001 taccataagt caagagacga tcaatgcaca tttttccgat ttggattcct aatctatccg
    12061 tgtagcctag atcatcgcca cgagcagaaa aagtagattc gacttctttg gataaagcat
    12121 cgccaatacc tgcccataag tatttatcag gtgcttcggc aataattgct gtatcaatga
    12181 aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc cattacgcct tcttcgttat
    12241 agatgacaca taccgcagtg acgggcgaac agttcgaacc aatagtggga aaggtgaaga
    12301 ctggtttagc tagtttttca ccaatgactt ttacagtatc gcaagcacgg ccaccaccga
    12361 ctgcaaacag gacgtctgct ttttggacac tttcatgctg gatcaactgt tcaatatttc
    12421 cataagttga attacccccg taccagagac tggcgacaac ctctatttcc tgtgtcaaag
    12481 ctacttttat tttgttttct gcctttgaaa gtgcagtctt ccctccaatc agcactactt
    12541 ttgtcccata ttctttgacg attccatttg ctttttcata cgcatctgtt ccaatggtat
    12601 aatttggtaa gaaaacactt tgactcatat tcatttcctt ctttcttcgt tgaaacgggt
    12661 taaaatttta aagagtgatc gatctgactg cggctgtaga tggagtagag gtgaacctaa
    12721 gacaggataa gtgatgaata aggtggcatg aacaccttga aatcatcaat aagtatccta
    12781 aaaacgaaca agtacaaaaa cattgaaaac gacaataatg aatttttgtt cctcttagta
    12841 tttctaccga ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa ttaagattat
    12901 tatatgaaaa caggttttat ttgtcaattc ataattggtt ttttttaaag caaaagataa
    12961 tctatatcat gatgaacaaa cagagttttt tcctccaatt gatggctttc ctgatctttc
    13021 ctattttgaa taaagtgttt ctgtataatt tttttatttt ataagtaaga taaataatga
    13081 aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt cgttttttca
    13141 aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca gtgaatttga
    13201 ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga cttggccgga
    13261 agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag aacgaatcgc
    13321 ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc ctcaatacgg
    13381 tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa agaaccaaat
    13441 aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag gaatcacgat
    13501 ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg acctgtatga
    13561 gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt atgaattcta
    13621 tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc
    13681 ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat aaaaagaggt
    13741 tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt acttcttgaa
    13801 tttttgtgaa attccaactt atttccgaag aagctacttt tggaacaccg tgtaccagag
    13861 gttgtgaaaa agtcgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcta
    13921 catttttgca taaatttggc ttaataccga gaaactggct tttgaacacc gtttattcag
    13981 ataagggact gtgacaagac ttttgtcaca gtttcttttt gtttattctt ttttatcttt
    14041 gtatggcaat aatagacaat ataacgaaaa ttatttaatg gcttttggaa gaccatggaa
    14101 aaccttgtac aaagctattt tttctgaaaa attccttgaa acctcacgaa aaaatcatta
    14161 ttttatgaaa aagacaaaaa aaagtgttga ttttttaata attattcatt aaactcttaa
    14221 acaacttcag aaaatattaa ttattctgaa aattccaata aaggggtacg taaaatgaaa
    14281 aagaaattta catttggttt ggttgcggta tgcggccttg tacttgctgg atgttatggc
    14341 ggcagttcag ccacaagcag tagttcagca aaaagcggtt cagcagatgg aggaggcgta
    14401 tttaacttag tcgtgcctca agaaatgcct acagctgact tatcagttgc aacagataca
    14461 atcagtttta cagcattgaa taacgtttat gaaggaattt accgtcttga tgaagacagc
    14521 aaaccacagc cggcaggagc tagcgagttg gcagaagtca gtgaagacgg attaacttac
    14581 aaaatcaaat tacgagaaga cgcaaaatgg tcaaatggtg atcctgttac agcagcggat
    14641 tacgtttatg gctggcaacg aacagtagac ccagctacag cttctgaata tgcttacctg
    14701 tatgcgccag ttgaaaatgc agaagacatt acagctggaa agaaagataa atcagaacta
    14761 gggatcaaag cagttggtga ctatgaatta gaaatcaaat tgacgaaaca aacaccatat
    14821 ttccaatatc tgcttgcttt cccatcattt ttcccacaaa aacaatccgt agtggaagaa
    14881 aatggggatg cctatgcatc tgcaagtgat aaagctgttt ataatggacc attcacatta
    14941 gaagattttg atggaccagg aacagataca gaatggactt ataagaaaaa tgatcattac
    15001 tgggacaaag atacagtaaa actttctgaa atcaaagtca gtgtagtaaa agaatcttct
    15061 actgcattga acttattcaa agatggccaa gcagatgacg tgatcttgtc tggtgagtta
    15121 gcacagcaaa acgcaaacga tccagcttat acttctgtta aagaagcgcg gacaagctat
    15181 atcgaattca atcaacgtga aaagaattca ccattcaaca atgtgaacct aagaaaagct
    15241 atttcttatt ctatcgatcg tgaagcttta gtaaaacaag tactaggtga cggatcagtt
    15301 gtctctacag gattgatccc atcagatatg gtaaaaagtc caacaacgaa cgaagacttt
    15361 gcaaaagaag ctggaaaatt agtttcttat gacaaagata aagcaaaaga atattgggaa
    15421 aaagcgaaaa aagaattggg cgttgattct ttagaatttg atttgatggc ttctgatgat
    15481 gattcatcga aaaaagtaat cgaatatgtt caaaactcaa tccaagaaaa cttggatggc
    15541 gtaacagtca aaccaacacc tgttccattc tctgtacgtc ttgaccgctc aacttcgggt
    15601 gattttgacg cagtactagg cggatgggct gctgactatg cagacccaag cagctttact
    15661 gatttgtttg tcacaggtaa ttcatacaac cgtggtcgct ggagcaatgc agaatatgac
    15721 gaagcagtta aagcagcttc atcaacagac gctggcgatg aacaagcccg ttgggatgat
    15781 ttccaaaaag caatcaagat catctctgat gatatgggtg tcgttccggt gtaccaaaaa
    15841 gcagaaggtc acctagtgaa tgaaaaaatc aaaggtatcg ttcaccatgc ggcaggcgct
    15901 tcatgggatt acaaatggac atacgttgaa gaataatcgt taataccgtg agcatgagaa
    15961 aaaacgaggc tgcggcaaat gtcgcaacct cgttctcata ttcacttatc ctaacaaaga
    16021 aaaaagcatg aaaatagcag aaagagcaat tagtattccg gcaatgatca gccaaaagct
    16081 gtacataccg tagctggcag acgagagcga ggaaaattct ggttttttct ttcggttcct
    16141 tgttcgtgca agatggaccg atcgctgaaa atgatcgaag aaacccgaag aaaagaccca
    16201 taaaaaaccg ccgataatca gaaacggcaa tgaccataag aatagatcat tagataattg
    16261 aatgagtgta aaggaatggt tgatgagatt ttttattaaa ataaccagaa tcagtagaaa
    16321 agctactgcg taggggatag attttttttt catttgattg ctcctttatt tttggttcat
    16381 tatctaataa tgtagcgaaa catctgtcag aagtcaaaag ctagggggaa acattagtga
    16441 atagttatat caaatatgta ttaaaacgtg tctttttcat gatcatcacg ctttggttaa
    16501 ttgcaacaat cacctttttc ttgatgcaat tgctgccggg cacgccatat acgaatcagg
    16561 aacgtttgag tcctgaaaca atcgagatgc taaataaaca agtcggtttg gataaaccag
    16621 ttatcgtaca atatgggatt tatctttcta atttgcttca aggagatttt ggaatttcct
    16681 tccaatttaa aaaccagcca gtcgcacatt tgttagctgg aagaatcgga ccatccttgc
    16741 agttagggtt acaggcaatt atctttggaa cagtcctagg gacgatttta ggaacgatct
    16801 ctgccatgaa gcaaaataca tgggcagata catcttctac actcgtagcg atattaggtc
    16861 gttccattcc aaactttgta tttgctgttt tattacaata tatctttgca atcaaattac
    16921 aagttttgcc aatcgcaaaa tgggatggct ttgtgtacac aatcttacca acaattgcgt
    16981 tggccatgtc gccgcttgct gactctgctc ggtttatccg tacggaaatg gtggaagtac
    17041 tgcatagtga ttatgtggaa ttagcaaaag caaaaggatt gagccgttgg gaaatcgcct
    17101 ttaaacatgg tcttcgaaat agtttgattc cattgatgac tttattagga ccgttagctg
    17161 tagcattgat gaccggatca ctggtggtag aaaatatctt tgctattcct ggtatcgggg
    17221 aacaattcgt taagtcaatc acaacaaatg attatcctac gatcatggct gtaacgatcc
    17281 tttactcatt tatgctgatt tttgttattt tgatcgtgga tctattgtac ggtctagttg
    17341 atccaagaat tcgtgtttca gaagggagcc gaggataaat ggaaatgata ccaaagcgtt
    17401 atgaaacagt tgcagatatc cctgcggatg aatttcttcc attacaaaaa aataccgaag
    17461 aagaacgtga acagattact gcaccttctt tgaattttat ccaagattca tggcgccgtt
    17521 taaagaaaaa caaagctgcc gttatttcaa tggttttact attggtcatc attttgatct
    17581 ctgtgatcac aatctttgtt tctcctcatg atccaactgc ccaaaacgtg gcttatatca
    17641 atttgcctcc tcgtattccg ggaatcaata tcgatggatt gaatggaaaa gcaatggttg
    17701 gtggtgaact tgttgataaa tatgctcaag caaacgtccc ttcaaacgta aatttctttt
    17761 taggaacaga cggtttggga cgtgatgtat taagccgctt atttatggga atgagaatct
    17821 cgttgctgat cgcatttatt gctgcattac tagatgtaac gattggtgta gcttacgggt
    17881 tggtttctgg tttgttaggc ggacgtgtcg ataacgtgat gcaacgtttt ttggaaatct
    17941 tatcaggaat tccaaacctc gttgtcatga ttttgatgtt ggtcgttttt gaaccaggaa
    18001 ttttttcaat cgtggctgct atggccatca caaactggat accaatggca cgtatcgttc
    18061 gtgcacaaac aatgaaatta aaagatcaag aatatgtttt agctggattg acattaggtg
    18121 aatcaagaat gaaaattgca ttcaaacata ttttgccgaa tatctctagt gtcattatta
    18181 ttcaaatgat gtttagtatt ccatctgcta ttttcttcga agccttttta agctttatcg
    18241 gtttaggtct tacgcctccg tcagcttcat taggaacgat gttgagcgaa gggtacaaga
    18301 cattcctgta tctgccttat ctattatgga ttccggcagt aactttatcg gttatcatga
    18361 ttggctttaa cttacttgct gatggattgc gtgatgcctt tgatcctaaa atgaaagagt
    18421 gaagacgatg acgaaaattt tagaagtgaa agacttagaa atctcttttg atacgtatgc
    18481 tggaaaggta cgagcgattc gcggtgtcaa ttttcatttg aataaaggag aaacattggc
    18541 gatcgttggg gaatctggaa gtggaaaatc agtgacgacc agaagcatca tgcgcttgct
    18601 gtcaagcaac gccaatatag atgctggtca aatcctcttt aaaggtaacg atatcgttgg
    18661 aaaatcagaa agagagatgc aaaaaatccg tggtaaagaa atagcgatga ttttccaaga
    18721 tccaatgacc tctttagatc cgacgatgcc cattgggaag caagtcgctg agtcccttag
    18781 aaagcataat aaagtttcaa aaaaagaggg acaaaaagct gcattagaat tgcttaaatt
    18841 agttggaata cccgaagctg agaaacgtat caacagttat ccgcatcagt tctcaggagg
    18901 tcagcgtcaa cggatcgtga tcgcgattgc actggtatgt tatccggaaa tactgattgc
    18961 tgatgaacct acaacagcgc ttgatgtgac tattcaagca cagattttgg agctgctgaa
    19021 agaaattcag acaaaaatcg atacatcaat tatctttatc actcatgacc ttggtgtagt
    19081 agcgaatgtc gctgatcgtg tcgctgtcat gtatggagga agaatcgttg aagttggaac
    19141 atcggaagaa attttctaca acccgcaaca tccctatact tggggactgc ttggttcaat
    19201 gccgacatta gatagtgcga acgatcgatt gtatgctatt cctggttctc caccggattt
    19261 attgaacccg ccaaaaggag acgcattcta tccaagaaat gaattcgcga tgaaaatcga
    19321 tgcagaacaa gaaccaccat tttttgaact atcaaaaaca cacaaggcag caacttggct
    19381 acttgctccg caggcaccca aagtgacacc gccagaagaa atccaacgtc gctgggctat
    19441 ctttaaagag aaacaaaaac atacttacgg tcaaggagga attgctgatg ccaaataaac
    19501 tacttgaagt taaaggattg aaacagtatt tcaatgtagg aaaaaagaat gaagtacatg
    19561 ctgtaaatga catcagcttt cacatttatg aaggagaaac attcggttta gttggagaat
    19621 ctggaagcgg aaaatccact actggccgta cgatcattcg cttgaatgaa cctactgatg
    19681 gtgagatatt attcgacggt caagatataa cgaagatcaa agataaaaaa ggtttgacga
    19741 aattccggca tgatgttcag atgatctttc aagatccata cgcttcgttg aatcctcgta
    19801 tgaaagtcag agatatcatt gccgaaggaa ttgatgttaa tggactagct aaatcatctg
    19861 aagagcgtgc aaaaaaagtc gatgatctat tgagaactgt tggattgaat ccaagccacg
    19921 gtactcgtta tcctcatgaa ttttcaggtg ggcaacgcca gcgtattggt attgcacgag
    19981 cattggcagt aaaaccgcgt tttatcattt gtgatgaacc aatttctgca ctagatgtgt
    20041 cgatccaagc ccaagtggtc aatttgttgc aagatctcca aaaagaacat caattgacct
    20101 atctatttat tgcacatgat ttgtcgatgg ttaaacatat tagtgaccga atcggtgtaa
    20161 tgcataacgg cttgttatta gagatgggaa caagtgatga aatctataat cacggtgttc
    20221 atccttatac agaaagtttg ttatctgcta ttcctctacc agatccagac catgagagaa
    20281 aacgcagaag aatcaaatat caacctgaac cagatgacgg acaagtacgt caacttcgtg
    20341 aaatcgcacc agaacatttt gtctatgcaa ctgagcagga agtcccatat tacgcaaaaa
    20401 aactaaaacg acaaaaggaa tctcttctgg ttgctaacta ggagggacat agatgctaaa
    20461 agcttttaaa ttccgtatgt atccgacaga agaacaaaaa cagcaattga ttcgtacttt
    20521 tggatgtgct cgatttacct ataaccattt gctgaaaaag cggcagaaaa gttggcaaca
    20581 aacaggagta gccaattttt cgctcacacc cgctacatta aaaaaagaat acccattctt
    20641 aaaagaagta gacagtcttg ctttagcaaa tgcacagctt aatttggatc gggcttttcg
    20701 caattatttc aagggacgtg ccagcttccc aaagcttaaa acgaaaaaaa gtatgtggca
    20761 atcttatacg accaacaacc aaacccgtac gatctatctg gaaaatggct acttaaagtt
    20821 accaaaacaa aaagaactaa tcaagataaa tcagcatcgg ccagttgaag gatcgattcg
    20881 ctccgctacg atttctgctc gatataatga agaattttat gtcgcactac tttgtgatgt
    20941 ttcgccagta aaaaaagaat ctttagctaa atggatcggg atcgcctatc atccgaagac
    21001 attgatccaa acatctcgac ctctagaagt aactttacca aaatttcacc agacagaaga
    21061 aaagctgcaa catgcacagc ggaaattgaa tgtgaaagtc cgctccgctc accatagaaa
    21121 gatcaggtta gatcaggcaa gcaattatca gaaacaaaag agaaaagtaa tggatcttta
    21181 tctgaaacaa aagaaccagc gagaagatta tcttgaacaa ttatctggga aattggtcaa
    21241 acaatatgac tacttatttg tagaatcttt tccaaaagaa gaggcacatg ctgatttttc
    21301 tattcacgac tggcataaat taataacaaa attgcgttat aagtcccaat ggtataataa
    21361 aaaatttctt ttgatcaata ccgacggagc agaagaaagc aattccgtaa gaaagagcca
    21421 agtagtagaa aaaatgggca gacattcggt tatcaaggga taaaaaatga atgaaactga
    21481 taggagcgtt agggttagcc ttgttaataa aaagtcttaa tgacttttgt ttctaggaat
    21541 ttttcttttg aagaaaagct cactaattat gacaaaccaa taaaacaata gcagaaaaag
    21601 ttcgtacgag ctttttctgc tattgttaat tttttattca gaaagtttac gaatacatca
    21661 aaattctgaa acaaaaaacg taactcacaa agagttgttt ttgcgttttt tcttgaatga
    21721 ttaggtctga atcttttgct cacaacctta ttcatggtgc cccggcttga gctgatagat
    21781 ttttgaagga cggccgattc ctacaggacg ttcaccgatc tcatcgaaat attggagcat
    21841 agctttctta aaattggaat gatcgatcgt tttataatcg atccctaaga atttagcaaa
    21901 tacttttcga gcttccgtaa tcgtgaaatc tttacctaaa acttgtagaa cttgcggctc
    21961 gtgttccatt ttattgacta cacggttgaa tgctttcaag atgatttgac tatggtcaaa
    22021 agccaaagta tctttgccag aagactctcc agtgactaaa tcaagcgaga tagccacttc
    22081 tccgctagat aaatggatct tgttactatg acgttctagt gtgaaccagc gtacttcctt
    22141 ggcatcatct ccggcgatta gcggttcttc tccaataaag gctaagtagc tgactgtgac
    22201 gacccagcct cgcggatctc gattaggcgt actgaatgta tgcagttgct caatattttc
    22261 ttttgaaata accacaccag tttcttcctt tgtttctctt aagacgcttt ctccagttga
    22321 ctcatccttt tggacaaagc caccaggcaa tgcccatgaa tttcgataag gatgcccttt
    22381 tctttggatc aacaaaactt tcagctgatc ttcttctttg ttatagcaca tcagtacgat
    22441 atcgacagtc aaagagggct tgtcatattc tggacgttcc tgtttttgat accagttaag
    22501 aaattcttct tcacttgctt tatgttcata atataatttt tcttcttctt tagaagcaaa
    22561 acggttcatc tgaccacctc cattgacagt aaaagtactt tttaccttat tgtagcagac
    22621 tctgaaaaaa aaccaaggat ttacttgaaa aatctaggtt atcatttaaa tatgaaaaat
    22681 aaatggaagt atgagattga ataaaaagaa aagcagataa aacggcaata ctttataccg
    22741 ctcttcacga atggcaaaga agccacaaat aacgatatat acgtcaatga taaaacgcaa
    22801 aatccaagct gctgtcataa aactgttcaa atgcccatgg aggttaaaat aaccatacat
    22861 acgaatataa aaatagctga atattaaatg tgctaatgaa agaaaagcaa tactaagtgt
    22921 aaaaaaaaga tgtttcttct gcatgattgt tccagtccct tactaagata gatagtcttt
    22981 attataccac gtgtgagggg agaaataaaa agccgttttt tagtagatat gaaacaaaac
    23041 aaaaacccgc atttattcaa cctattcatc cttttaaagg atagattgaa cgtagataga
    23101 agtcgtttat ccagtctttt tcattttgaa aaaaagctaa cagcatgctt tttttatggt
    23161 atactagcaa ggaaacgaat tcgcatcatg cgataaagga ggaagagccc cgtggcagaa
    23221 aaggaaactt tttatatcac tacgccaatc tattatccaa gcggcaaact tcatatcggc
    23281 aactcgtata ctacgattgc atgtgacgca attgctcgtt ataaacggtt gatgggcttt
    23341 gatgtgtttt atctcactgg agtggatgaa catggtcaaa aaatcgaaac aaaagcagaa
    23401 gaactaggtg taaaaccgca agaatatgta gataaaatgg cggctgatgt caagaaatta
    23461 tggaaaacgc ttgatatcag ttatgataaa ttcattcgca cgacagatga ttaccataaa
    23521 gcagctgttc aaaaaatatt tgatcgttta ttagaacagg gagatattta cttaggtgaa
    23581 tatgaaggct ggtactctgt ttctgacgaa gaattcttta cagaaacaca acttgcagaa
    23641 gtgtacaaag atgacgaagg taacgtgatc ggtggtaaag cgccaagtgg acacgaagta
    23701 gaattggtaa aagaagaatc atacttcttc cgcatgagca aatacgcaga ccgtctattg
    23761 gaatactatg aagaacatcc cgaattcatc cagccagaat ctcgtaaaaa cgaaatgatc
    23821 aacaacttta tcaaaccagg attagaggat cttgcagttt cacgaacaac ttttacttgg
    23881 ggagtaccag tcaagagtga tccgaaacat gtggtgtatg tatggatcga tgcattatcc
    23941 aattatatta ctgcactagg atatggctcc gaagatgaaa gtctattcga aaaatactgg
    24001 cctgcagatg ttcagatggt cggtaaagaa atcgttcgct ttcatacgat ctattggcca
    24061 atcatgctga tggcccttga tttgcctcta cctaaaaaag tatttgggca tggctggcta
    24121 ttgatgaaag acggaaaaat gtctaaatca aaaggaaatg tcgtttatcc tgaaatgctt
    24181 gtggaacgtt atggtttaga tgcactccgt tattatttgc ttcgtgctgt tccgtttggg
    24241 tcagatggtg tctttacgcc agaagatttt gtttcccgtg tcaattatga tctggcaaat
    24301 gatctaggga acttattgaa tcgaacgatt gctatgatca acaaatattg tgatggactc
    24361 gttcctgact atgcctcact tgtcactcca tttgatagtg aactgtctac gacggctgct
    24421 aacgttgtcg gacgttacca tgatgcaatg gaaaaaatgg aattcaatac agcattggct
    24481 gaaatctggg tgctgatttc tcgtgcaaac aaatacattg atgaaacaga accatgggta
    24541 ttagcaaaag acgaagaaaa gaaagctgaa ttgaatagtg tcatgatcca tttagctgaa
    24601 tctttgcgta tcgcagcgat attacttcag ccaatcatga cagaaacgcc agagaagatc
    24661 ttcaatcaac taggattgga ctctgaaaca atgaatcttg aaggattaca ctttggcgaa
    24721 tttccttcag gaacaaaagt agttgcaaaa ggtacaccaa tcttcccacg tttagatatg
    24781 gaagaagaag ttgcttttat tcaagaaaaa atgtctgaag gtactcaaac aaacgaagat
    24841 acagtcaaat gggatccaga agaaacagaa cttgtttcaa cgaaagaaaa acaaatcaaa
    24901 tttgatgtat ttgaaaaagt agaactaaaa gtagcagaag tcatcaactg tcaaaaagta
    24961 gaaggggcag ataagttgct gcaattccga ctagatgctg gagacagcca agaccgtcaa
    25021 atcttgtcag gaatcgctga attttatcct gatccaagcg aattgatcgg gaaaaaagtc
    25081 gtcattgtag caaatctgaa accacgaaag atgcgtggac aaatcagtca aggaatgatc
    25141 ctttctgcag aagcaccaga tggctcttta caagtaatcg aagcaccaaa atccatgcca
    25201 aatggctcag aaatcgcata gaaattcaaa gggggtatga cactagttgt gtcatacccc
    25261 ctttttccga aatttgagaa gcaattttcg taaattcctt cttattactt gaagccaatc
    25321 atataataca gaaacagaga actatccaaa ataaatccta gaaaaagaag tacgagcttc
    25381 gctgattttt gttaatttag aataatacta ataggaatcg taaaaacaga aaaaaagcat
    25441 aatgataaag ggtcgggcag attttaattg attcatctgg tactcttttt tattgtcctt
    25501 acattcataa agtttgaatg caatgacttt attacatcta tatttatatc taacagtagc
    25561 gttgatgtgt taattataag ttctatcact ttatgttcaa ttagtttgtg ctcttatatg
    25621 tgtgattgaa aatatagata aaaaaacaat catcggcttt gacttcgatg attgttttct
    25681 ttattttgta aaaatcctat cacttgattt tatggtaacc ttttattttt gtcttcaaac
    25741 ggcttggtaa gagagcagta agcagtgcaa atgggatcga aaagacatac cagaaaatcg
    25801 tacgtccgat accataatgt tgatagagct ttgtcaacat cacgtgtaac aagatcaacc
    25861 ctaaaagcaa ggagccagct ataaaccaat gattatccca atgcatgact tcactaagtg
    25921 ccatcactcc aaaaggatag aaataaaaat agatcccgac taaggtcatc cgccagttct
    25981 ttgtaaatgt tccgatattc aataaaaaga aaatcgctga aagcaaaata ccgataggcg
    26041 gaaaaagccc aaccaaaaag gaatacaaca tcccccacaa gatcatcaca ggacctttga
    26101 tcaatgcaac gattgcgatg aacaaagtca gcaatattag tttttgtgat aacgttgcaa
    26161 cagtcattaa tccaaataaa attaatgtga accaatgcca atgggactgc tgttgttctt
    26221 tacgggaaaa atcgacccgg ttggataaat aatctgctaa tttcggataa gaacgcttaa
    26281 aatccataaa ctgttcctcc ttcgtggaaa aggtaggttt agtatacgaa aaataaaaaa
    26341 gaaatacatc cgtcttaaga gggagttttt cactgaaagt ggagaaaaaa gaatggtaaa
    26401 ttaaaatatt ctcagttata ctgaaaatta taccaaattt caaagaagaa agaggatggg
    26461 aatgaaaggc aaactaaaac gagaaatcaa tctgtttggc gcattagcta ccgttatggg
    26521 aaccgtgatc ggtgcaggcg tcttcttcaa aactgctgct gttactgcta gtactcagtc
    26581 tacaagtttg acattgctag cttggctttt gggtggtttc ttaacgatct gtgctggatt
    26641 gacagtggct gaattagcta cagcaatccc ggaaactggt ggagctgtaa aatatattga
    26701 agctgcgtat ggaaaattgc ccagcttttt attaggatgg gcgcaaagct tgatttactt
    26761 tcctgctaat attgctgcat tatcgattat ctttgcgaca caattgacga acttattaca
    26821 attatctacc gattatcttt tgttgattgc agtgatcacc gctgtttctg tgacagggtt
    26881 gaatttgtta gggacaaaag tcggtgcttc tgtacaatca gtgacgttga ttgttaaatt
    26941 aataccaatt gctgtgattg tcatatgggg attattgacc ccaggacaag gaactgtcca
    27001 gttatttcca atcgaagctg gaaaagatgt tacttttgtc gaaggattga gcagtgcgtt
    27061 gttagcaacg ttgtttgctt atgacggctg gttaggtgtc ggtgcaatgg caggggaaat
    27121 gaaacggccg gaaaaagatt tgcctaaagc aattattctt ggtttgagtt ttgtaacagt
    27181 cgtttatcta ttgatcaatt ttgttttttt gaaaactttg ccgattgatc atcttgccgg
    27241 taatttgaat gcagcttctg aagcatcaga cgtcattttt ggaggaatcg gtggaaagct
    27301 ggtcactatt gggatcttga tctcggtata cggtgcattg aacggttata cactaactgg
    27361 gatccgcgtg ccttatgcga tggctttaga agacgagcta cctttcagta aacagctgac
    27421 aaagctttca aagaagttta cggttcctta tgttcctgcg gttttccagt tagcagtagc
    27481 ttgtattatg atgagccttg gttctttcga ctttttaaca gacatgttga tttttgtgat
    27541 gtggctattc agtttactga tttttatcgg tgtctttatt ttaagaaaaa aagcgccgga
    27601 attgccacgt ccatataaag taccacttta tccaatcgtt ccgattattg cgattctcgg
    27661 tgctattttc attttaggta tgacgatgat gacgcaaacg aaattagcat tgatcggtat
    27721 aggtgttacc ttgataggta tcccagtcta ttatcaaaaa aagaaaaaac gctcagaaga
    27781 gtaaaatatt tcaacaacag acagaaaaag tacgaactag gtcctctaga tcttcgtgct
    27841 ttttctattt caataaaaca agttcttcta ttttgatttt tgctacttct gcaagtagtg
    27901 cttatgtgct aaagtaagaa tgaaaaggag agataaacat gatatttgat tctcataccc
    27961 atttaaatgc tgaacagttc aatgaagata ttccagaaac gattcagcga gcacaagaaa
    28021 tgggggtaac gaaaatggcg gtcgtgggct tcgatacgcc aacgattgaa aagtctttgc
    28081 aattaagcca tgactatcca aatatataca gtattatcgg ctggcatccg acagaggctg
    28141 gcagttatac gaaagaaatc gaaaataaat tacaagaaca attgacttta ccaaaggttg
    28201 tagcattagg ggaaattggc ttggattatc attggatgga agacccgaaa gaagtccaag
    28261 ataaagtatt tcgcagacag attgctatcg caaaagaaat gaacctacca atcagtattc
    28321 atactagaga agcacttgaa gacacgtatc aaatcctaaa agaagaagat gttcgagata
    28381 tcggtggaat catgcatagt ttcagtggtg attatgaatg ggcaaaacga tttcttgact
    28441 tagggatgca tatttcattt agcggtgtcg tcactttcaa aaaagcatta gacgtccaag
    28501 aggctgcagc aaatgtacca atcgaacgat tacttgtgga aacagatgcc ccttatctgg
    28561 caccagttcc ttttagagga aagcgaaatg aaccaggcta tactcgttat acagtagaaa
    28621 agattgctga acttcggaat cttccgacag aagaagtggc tgcccaaaca tggacaaatg
    28681 cgcaccgatt atttggtcta agcgaacatg gataagatga agatccaaga gattatagtc
    28741 gttgaaggaa aagacgatac aagaaggctg cgggaagtag ttgatgcaga tacgatagaa
    28801 acaatagggt ctgcaatcaa tgaagagatc ctgatgcaaa ttgaacatgc ccaagagact
    28861 cgtggagtga tcgtttttac cgatcctgat ttttcaggag aaaaaataag aaaaatcatt
    28921 atggaagctg ttcctcaggc aaaacatgct tttttgccgc gcacacaagc taaaccaaag
    28981 aaaaagggaa gccttggtgt cgagcatgca agtgatgctg ctattttaga agcattgaaa
    29041 aaggtcgtga cgccagacat ttcccaaacg gctgtttctg aaatcactag acaagacctt
    29101 ttagactatg gtttaatagc tggggcacaa gcaaaagaaa aacgagaaaa actaggtgac
    29161 gagttgcgca tcggttacac gaacggcaaa caattggaaa aacgtttgaa gatgtttcgt
    29221 attacgccga aagaactcgc ccaagcaatg aaaatagtgg aggaacaaca tgagtgaata
    29281 tcgtgaaatt gcgacaccct ctagaacaaa agagatttta ttgaaacatg gtttttcatt
    29341 taaaaaaagt ttaggacaaa attttctgac tgaaccaaat attttaagga aaatcgtaga
    29401 aactgcgcaa atagatgaac atacgaacgt gatcgaagtt ggaccaggaa tcggtgcatt
    29461 gacggaacaa ttagccaagc atgcaaaaca agtcgtagca tttgagatcg atgatcgttt
    29521 gatccctgta ttggctgata cgatgcaacc ctatgacaat gtgactattg tacatcaaga
    29581 tattttaaag accgatttgt caacagctgt tagagaaaca tttcatgagg agcttccgtt
    29641 aaaagtcgta gcgaatttac cttactatat tacgacaccg atcatgatgc attttcttga
    29701 atcagatttg gttgtggatg aactagtcgt catgatgcaa aaagaagtag ctgatcgtat
    29761 ctctgctgaa ccaggaacga aagcttatgg ttcattatcg attgctgttc aatattatat
    29821 ggaagccagt ttagcattca tcgtaccaaa aacagtattt gtccctcagc caaatgtaga
    29881 ttcagcgatt ttgaaactga caagaagaga tataccggca gtcgaagtga cagacgaaaa
    29941 agcatttttc cgattgacaa aagcggcatt tcagcaaaga agaaaaacgc tttggaataa
    30001 cttgcagcat tcgtatggca aagatgacca gacaaaagca tggttagcaa aaagcttgga
    30061 aacagctgga atcgatccaa aacgtcgtgg ggagactttg tcattacaag aattcgccgc
    30121 attaagcaat gcgatgtcag aaaatatgca gtaaaaaaag aggttgtgac agaagttgtg
    30181 aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatgtttt
    30241 tcgtttgttt gggcttatta ccgcagatgc aagcttttga acaccgttta agaaagaggt
    30301 tgtgggcctg ccgtttaact ctaagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    30361 tttttgagtt agttggtctt attacttgat gcaagctttt gaccactatt atgccgaaca
    30421 aggagatgtg acacaaattt tgtcacatct cctatttttg tttcttgatc aaaaaattct
    30481 tatacttgat actcacactc attttgtatg gatttagaaa agaatatgtg tcttgacagt
    30541 tattagtaag tgagttatac taagtcgaca tttatgtgtc aagacatatt aggagagaga
    30601 aaaaatgaga aaaaattcag ttcgaaaact aacgatttct gctttattga ttgggatggg
    30661 ggtcattatc ccaatggtca tgcctaaaat catgattcca ccagcttcct ttactttggc
    30721 gtctcatgtg ccattattta ttgcgatgtt ttttacacca ggagttgcag tggctgttgc
    30781 gctaggtacg acatttggtt tctttttaac aacgccggtt atcatcgcat tgcgagcatt
    30841 gtcacatctt gtgtttgcgt tgattggggc ttggtacttg caaaagcatc cgagtatcgt
    30901 tttaaaaaat ggacagttta cttttgctaa ttggcgtttc caagggttca attttgtgat
    30961 cggtgtgatt cattctttag cagaaatgct agtagtcagc atcttttact ttattggtaa
    31021 tatgcctgaa acatattatt ctcaaggata tttttataca gtattcatct tgatggggat
    31081 tggtgggctt atccacagtt tagttgatta taatattgct tattttatag caggaacact
    31141 cagcaaatat tttgatatcc ccgtatttac ggcagcaaaa aaagaaaatc ggaaagagtc
    31201 gattcaaggg tacgcgaaga aaacgacata ttagacatga tctctcaaaa aaaaaactct
    31261 catctagtaa tggataataa atcctctaag atgagagttt tttgtcctaa aaagagctgc
    31321 accaacatct tctggcacag ctctaacagg gggcaattta tggaagtttg atgacttgtt
    31381 ctgtttcttt atcaaagaag tggcctttgc tgatgttgaa ggcaaggtcg actatttcgc
    31441 ctggtttatg gaaatctctt gcatctactt ttgagatgaa ctcagtagaa ccagtttttg
    31501 tgtaaagcat cgtttcagct ccaagtaatt cggaaacgac aacttctgat ttcactacgg
    31561 cttcaggtga agcttcaagg gctacttgtt cactgtggat gtcttctgga cgaataccga
    31621 agataacttc ttttccttca taaccttttt caactaatac tttgtttttt ccttcaggaa
    31681 tacgaagctt aagcccatgt ccatctgtga taacaccatt tttcaatgtc acattaaaga
    31741 agttcatggc aggagatcca ataaagccgg caacgaacat attgacgggt gtatcataaa
    31801 cttctttcgg tgtaccgatt tgttggataa atccgtcttt catgataacg attcggtcag
    31861 ccatagtcat tgcttctgtc tgatcgtgag taacatagat ggtcgttgtt tcaagacgct
    31921 ggtgaagttt ggcaatctca gcacgcatgg ctacacgtaa tttagcatct aaatttgata
    31981 atggctcatc cattaagaag acttttgcat cccgaacgat tgcccgtccc aatgccacac
    32041 gttggcgttg tccaccagat aaagcagctg gttttcgttt caagtattct gtcagaccta
    32101 agatctctgc tgcattgtcc acacgttttt tgatgtctgc tttatcatat ttacgtaatt
    32161 tcaagccaaa agccatatta tcaaaaacag tcatatgagg atacaatgcg tagttttgga
    32221 aaaccatggc aatatcacgg tctttaggtg ccacgtcatt catgactttt ccgccgatat
    32281 ttagttctcc ttcagagata tcttccagtc ccgcgatcat tcgcaaagta gttgactttc
    32341 cacaaccgga aggaccaaca aaaacgataa attcacggtc tttgattgat agattaaaat
    32401 cagttacaga ataattttct gcattgtcat attttttata tacgtttttc agagccattt
    32461 ctaccataat acgttccaca ctttcttttt tagttttatt aactatttaa agtataaatg
    32521 aaagcgttaa ctaacttcaa tgtcatgttg aacaagaatt ttttcttttt tttgtcaatg
    32581 tgctagtttt aagctttaat aaatatccat aagaaataaa aactgataca ctggagtaaa
    32641 taagtatgtt aacaatatca aaaggagtgt ctcttatgaa aatcgctttg atcgctcatg
    32701 accgaaaaaa acctatgatg gtgaaattag caacagcgta taaaacaata ttggaaaaac
    32761 atgaactttt tgctaccggg acgactgggc agaggatcac ggaagcaacc ggcttgccga
    32821 tccatcggtt taaatccgga ccgttaggcg gggatcagca aattggagcc atgatttcgg
    32881 aggatcagtt agatttggtg atttttcttc gagacccgct ggctgcacag ccccatgaac
    32941 cagatgtaac agcacttatc cgtttatctg atgtctatga gattccgtta gctacgaata
    33001 tcggaacagc tgaggtgcta ctaagaggac tggaagccgg cttcctagat tttcgaagaa
    33061 tcgtccatga aatagacaag catccacttt cattttgatg gataagcgta tggaaaaata
    33121 acgtttaatg ttcgcctata tgtagctata aaagctaaac aaactgataa tatgcgatat
    33181 aatacgaacg tattggataa tataaacaat aatattagtt tttcgtattt gcagtaaatg
    33241 tgaaaatatg ctatagtttc tgtgggaaaa gatcccaaaa ataatacaga gtagaaaata
    33301 aagcgttaag tgctaagagg atgggatgtt gcctcttgga cgaaggacgt aatgttcgcg
    33361 gttttatttt cgcattcgct gcatgatacg atgtagcagc tcttaaagga gatgctaaaa
    33421 atgaagaaac accggttaga cgcacgtatg attgctatta tgggcttatt gattgcattg
    33481 atggtcactt tgtcccgact ggtagcaatc gagaccccat ttattaaaat cagtgtcaca
    33541 tttattccac aagtgatcat gggcattctt tttggtcctt tttggtcagg gataggcgca
    33601 gttttagctg atttagtagg aatggccctc ttctcgaaat ctgcattctt tattggattt
    33661 acattgaatg catttattga aggagcaatc tacggattct ttttctatcg aaaagagatc
    33721 acttggaaaa atgccattct ggctacgctt agtgtcacgc tgatcatcaa cttatttttg
    33781 acaccattat ggcttgcgtt gatgtaccat gtgccattgt tcagctgggt cgtttgggcg
    33841 ccccgcctgt taaaaacagt aatatggtta ccgattcaat caattgccat ttattacgta
    33901 ggacgtagta ttccttacaa aaaaatttta agaagtttag cgatccacgc taaataaaaa
    33961 acaagcagtc ttcccatccc agaagactgc ttgtttttat tgtgctgtga attgtgcttg
    34021 atagagccgt cggtaataac catctttttg aagcagctca tcatgggtgc ccatttcgac
    34081 aatagctccc tgatccatca ctaatatctg gtccgcattt ttgattgtgg acaggcgatg
    34141 ggcaatcaca aaactggttt ttccttccat cattgctaag aaggcttgtt ggattttttt
    34201 ctctgtcaat gtatcgactg agctggtagc ttcatctaag atcaacatag ggggattact
    34261 gatcatcgtt cgagcaatcg tcaacagttg gcgttgtcca tctgatatct ttaacccttg
    34321 accgccaatc ttagtatcta atttttccgg caaacgttct acaaattcat acatataaga
    34381 ggcttttagg gcatcattga tttctgcgtc acttgcttcc ggattcccaa aacgaagatt
    34441 ttctcgcaat gtactgtcaa atagccaagt atcttgtaac accattccaa aacttttacg
    34501 taatgtatca cgttggatag agcggatgtc atggtcatcg actgtaattg ttccttttgt
    34561 tgtgtcataa aaacgcatca gcaaattaac aagtgtggat tttccggccc ctgtttttcc
    34621 tactatagca atggtttgac ctggataagc agtaaaattg aaatcttcga tcaaacgctg
    34681 cttggggtca taagaaaaag aaacatgatc aaaagagact tttcctttga tatttttggg
    34741 ttccaaggca taggcatctg gcaaatcagg cttttcttct ggctgatcga tcaaatcaaa
    34801 tgctcgttct agaccagaaa aagcggtttg cagttgtgtc gtgattccag aaagttcgat
    34861 aaatggttta gaaaactgac ttgagtagat cgtaaagctg gaaatcacac caacagtgat
    34921 tgacgaacca ctcttcaatg ccaataggcc gccaactaaa ccaatcgata aataagcaag
    34981 gtgatctaca aaacgagaag acgggtttgt caaggaagaa gaaaattgtg cccgttgtcc
    35041 tttttcatat agatcagcat ttagcagttc aaattgctgt tgactagctt cttcttgctg
    35101 aaaagctttc acgattttct ggttaccgat tcgttcggtc acaaagcccg agatatcccc
    35161 aataatcctt tgctgtctat cgaaatttgt ttgtgaagca cgagcaacga tccaactcac
    35221 aaggaaaata attggtgtag aacaaagaac gaccaatgtc agaagtggac ttagacgcat
    35281 cataaaaata aacgaaagaa tgatgattgc cactccagaa aataattgat tgaaggccgc
    35341 agaaattgca atggaaatat tgtccatatc atttgtaaat cgactgacga tatttccgtg
    35401 ggagttttga tcataataac gaagtggcaa tcgattcaga tgagcaaatg catcttttct
    35461 taattttgca acggataagt aagctaaccg attgcttaac acttggatga gccattgact
    35521 gactacgttg attaaaacga taccaccgaa tagaataagt aaatggaaca gtcgattgaa
    35581 gcggacctgt ccttctccaa tcatttgatc gatactttga ccaatttgta acgtcatcac
    35641 aactgttgca atcccattga tgattcctag tatcacagct agccaaatgt cttttgcata
    35701 ggcagtaagg taaggaatga atcggcggag cgtctgcaag gataattttt ttactttcat
    35761 aactattctt cctcctgtga tgcgacgatt tcctgatatt ctgctgattg agcaagtaat
    35821 tcttcatggg taccctgagc agcaatctta ccttgatcca tcactaagat atgatgtgct
    35881 tcttgaatag agcggacacg ttgggagata ataatcactg tactgccaag ataagtctgt
    35941 agcgccttac gcaaatccaa gtctgtttga taatccaacg cacttaaaga gtcatctaaa
    36001 atcagtaatg ctggcttggc aatcaaagct cttgcaatcg tcagtcgctg tttttgtccg
    36061 ccagagaaat ttttcccatt ttccatgact tttgtatcta aaccatttgg aagctgacgg
    36121 acaaattctt ctgcctgagc gattttcaga gcttcccaac attctttttc tgtggcgtct
    36181 ttcttacccc attgaaggtt ttcacgaata gtcccggtaa atagtacaga agtttgggga
    36241 acaagagcaa tttgctgacg gagatgatcc agctgccaag aacgaacatc cattccgtcc
    36301 attataactg taccggaact gacatcatag aaacgcggaa tcaattgtgt caaagtcgtt
    36361 ttcccgcttc cggttggacc aatgattcca aggactgtat ttcgcttcag ctgaaatgta
    36421 atattttgga gagaaagacc agaatcagga gtataacgga agtcaacatg gtcaaaagcg
    36481 ataattgctt cagaagaaaa atcgggagta gtactgacct cagcttcaag aatcgaattt
    36541 tctgtgtcta gcacctcttt cacccgattt cctgaagctt cggcacgtgt gaagatcacg
    36601 acgaggtttg aaacaacgat cagtgccaaa agcatttgat tcatataatt gatcaaagca
    36661 agaacttgtc cttgttgcaa gctaccaata tttactttga tcccgccaac tgtcagcaaa
    36721 cagatgatcc cgacattcat gatcaaggtt gtagcaggtg ataatagggc agagatattg
    36781 gaaacacgta aataattgtt cgctagatca tttgtgcttt tatcgaactt ctctgtttct
    36841 ttttttgtct gcgcaaatgc gcggatgaca cgaacaccgc taagattctg actaacgtgt
    36901 tcattgagct gatctagttt ttcttgcact ttttggtaga gaggaacggt ttttttgatg
    36961 ataaaaaata aaatgattga gaaaataggc aataatgcta agaaaaacag acctacttcc
    37021 cagtcgatca caaaagccat gaccactgac ccgatactta aaaacggtgc tctgatcaat
    37081 aaacgaatga ccattgctaa agctagctga agctgattga tgtcgtttgt cattcgggtg
    37141 atcaaggtat ctgttccaaa atgattcaac tcgttgtgag agaaagtatt gatttttttg
    37201 attagctgat tacgtaactc tgtaccaaat ccttgtgaag ctactgaagc atagtattgg
    37261 caaatgatag cacaaatcag accaatcaca gacatagcga ccatccataa gcccatttca
    37321 atgatttttc ctcggtcatt catttttagt ccattatcga tcaaggaagc catcattaaa
    37381 ggcaggacaa gttcgaagac agcttcaaga aatttgaaga acgggcccaa aatgatttgc
    37441 ttacgatagt cttttgcata acgtaataac tgaatcatat gattcctcct attatttatt
    37501 tgtccacaat tgaaaaaaca agtactacca cattgtagct taggaaattt agaatgaaaa
    37561 gagaacaagc cttttcataa atgaaagttt tattatgaag aagctttaga aattgaatag
    37621 actatttttt tt
//
LOCUS       DAZKMB010000014        36691 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000014 DAZKMB010000000
VERSION     DAZKMB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.014"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..3347
                     /locus_tag="ACG8V3_000657"
     CDS             69..3347
                     /locus_tag="ACG8V3_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0299221.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3888..5795)
                     /locus_tag="ACG8V3_000658"
     CDS             complement(3888..5795)
                     /locus_tag="ACG8V3_000658"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0299222.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5925..6800
                     /locus_tag="ACG8V3_000659"
     CDS             5925..6800
                     /locus_tag="ACG8V3_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0299223.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6880..7854
                     /locus_tag="ACG8V3_000660"
     CDS             6880..7854
                     /locus_tag="ACG8V3_000660"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0299224.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8034..9179
                     /locus_tag="ACG8V3_000661"
     CDS             8034..9179
                     /locus_tag="ACG8V3_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0299225.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9316..9660
                     /locus_tag="ACG8V3_000662"
     CDS             9316..9660
                     /locus_tag="ACG8V3_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0299226.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9931..11217
                     /gene="efmA"
                     /locus_tag="ACG8V3_000663"
     CDS             9931..11217
                     /gene="efmA"
                     /locus_tag="ACG8V3_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0299227.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11355..11954)
                     /locus_tag="ACG8V3_000664"
     CDS             complement(11355..11954)
                     /locus_tag="ACG8V3_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0299228.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12358..13479
                     /gene="mnmA"
                     /locus_tag="ACG8V3_000665"
     CDS             12358..13479
                     /gene="mnmA"
                     /locus_tag="ACG8V3_000665"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0299229.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13725..15146
                     /gene="arcD"
                     /locus_tag="ACG8V3_000666"
     CDS             13725..15146
                     /gene="arcD"
                     /locus_tag="ACG8V3_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0299230.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15160..16278
                     /locus_tag="ACG8V3_000667"
     CDS             15160..16278
                     /locus_tag="ACG8V3_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0299231.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16338..17630)
                     /locus_tag="ACG8V3_000668"
     CDS             complement(16338..17630)
                     /locus_tag="ACG8V3_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0299232.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17780..18340
                     /locus_tag="ACG8V3_000669"
     CDS             17780..18340
                     /locus_tag="ACG8V3_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0299233.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18469..18846
                     /locus_tag="ACG8V3_000670"
     CDS             18469..18846
                     /locus_tag="ACG8V3_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0299234.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18779..20851
                     /locus_tag="ACG8V3_000671"
     CDS             18779..20851
                     /locus_tag="ACG8V3_000671"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0299235.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21062..22009)
                     /locus_tag="ACG8V3_000672"
     CDS             complement(21062..22009)
                     /locus_tag="ACG8V3_000672"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0299236.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22255..22719
                     /locus_tag="ACG8V3_000673"
     CDS             22255..22719
                     /locus_tag="ACG8V3_000673"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0299237.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22781..23824)
                     /locus_tag="ACG8V3_000674"
     CDS             complement(22781..23824)
                     /locus_tag="ACG8V3_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0299238.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24201..25166)
                     /locus_tag="ACG8V3_000675"
     CDS             complement(24201..25166)
                     /locus_tag="ACG8V3_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0299239.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25400..26245
                     /locus_tag="ACG8V3_000676"
     CDS             25400..26245
                     /locus_tag="ACG8V3_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0299240.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26436..27623
                     /locus_tag="ACG8V3_000677"
     CDS             26436..27623
                     /locus_tag="ACG8V3_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0299241.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27629..27985
                     /locus_tag="ACG8V3_000678"
     CDS             27629..27985
                     /locus_tag="ACG8V3_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0299242.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27987..28325
                     /locus_tag="ACG8V3_000679"
     CDS             27987..28325
                     /locus_tag="ACG8V3_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0299243.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28401..28657
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28720..29754
                     /locus_tag="ACG8V3_000680"
     CDS             28720..29754
                     /locus_tag="ACG8V3_000680"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299244.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29747..30433
                     /locus_tag="ACG8V3_000681"
     CDS             29747..30433
                     /locus_tag="ACG8V3_000681"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0299245.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30457..31305
                     /locus_tag="ACG8V3_000682"
     CDS             30457..31305
                     /locus_tag="ACG8V3_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0299246.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31502..32275
                     /gene="sufC"
                     /locus_tag="ACG8V3_000683"
     CDS             31502..32275
                     /gene="sufC"
                     /locus_tag="ACG8V3_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0299247.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32288..33574
                     /gene="sufD"
                     /locus_tag="ACG8V3_000684"
     CDS             32288..33574
                     /gene="sufD"
                     /locus_tag="ACG8V3_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0299248.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33571..34806
                     /locus_tag="ACG8V3_000685"
     CDS             33571..34806
                     /locus_tag="ACG8V3_000685"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0299249.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34793..35263
                     /gene="sufU"
                     /locus_tag="ACG8V3_000686"
     CDS             34793..35263
                     /gene="sufU"
                     /locus_tag="ACG8V3_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0299250.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35268..36659
                     /gene="sufB"
                     /locus_tag="ACG8V3_000687"
     CDS             35268..36659
                     /gene="sufB"
                     /locus_tag="ACG8V3_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0299251.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT        11994 a         6781 c         7867 g        10049 t
ORIGIN      
        1 tgacagtaaa aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag
       61 gaatacactt gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc
      121 tcagtacccc ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga
      181 acgaagaaaa tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa
      241 ctagtgaaga tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg
      301 aaaagacaaa aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag
      361 aaagcacaag tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt
      421 ccacgaataa cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc
      481 aagcaatccc caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga
      541 cctatacgaa tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc
      601 atttgtttgg gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat
      661 atgtctggga ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc
      721 gacttggatt ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc
      781 cagctcctgg gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg
      841 aaaaagaaac aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca
      901 cgaactcgat tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat
      961 attattacga tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa
     1021 aattatacgc ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga
     1081 cgatcaaggt agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc
     1141 aagaaagacc tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag
     1201 gaaaccaaga tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa
     1261 ataaagcaac aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg
     1321 aatttgatta tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc
     1381 cagataaaaa ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga
     1441 ttcttcatac agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag
     1501 ggtcagatgc aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat
     1561 acgcatatgc tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat
     1621 tgacgattcc aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga
     1681 aaaatccaga aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac
     1741 tgttcagtca ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa
     1801 aaggattaca gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg
     1861 gaaatacagg aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc
     1921 ataacttagt caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg
     1981 atggatttgc ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt
     2041 atcataatgc agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa
     2101 aagtaatcat caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag
     2161 aaggtaacgg taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga
     2221 tcaatagttt gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata
     2281 ttatcgtcaa tagcagtaca agttttaata acggttcata taatgtggct ctttatacct
     2341 cttcagcagg aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc
     2401 ttgagatgag agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt
     2461 tgaactacta ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat
     2521 caaaagattg gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc
     2581 gctatgaaaa tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc
     2641 aaacagaaaa ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag
     2701 aaacctcacc gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa
     2761 aaccaaatga acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg
     2821 cagaaggaaa tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa
     2881 atcaaggaac gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg
     2941 aaacacagtc aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag
     3001 gagaatatac gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg
     3061 ctcatacaga atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc
     3121 ccccgggaaa tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac
     3181 caaatggaaa accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc
     3241 aaggactccc aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt
     3301 tgttaatcgg tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc
     3361 ttttatgatg taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag
     3421 atgtctcact tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct
     3481 gttcttatta cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat
     3541 cagctgaccc tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt
     3601 ttttctctaa tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa
     3661 aagccagttt ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa
     3721 aatttttctt actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc
     3781 agaagtaact tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc
     3841 caaattcctt cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc
     3901 actctttttt ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag
     3961 aattcgtcaa ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg
     4021 atttgcagta aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa
     4081 ttgacaaaga attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag
     4141 cttagaccgc ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg
     4201 tcagcaaaag gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt
     4261 gtaagatctt cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg
     4321 ttattatcaa atgcatggga aatctcatga gcgataacag ctccgattcc accataatta
     4381 gcagagcttg attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg
     4441 atcacattcc gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct
     4501 gaacgatcca ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa
     4561 acaagagtat ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg
     4621 aataatgctg ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg
     4681 acggcttttt cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa
     4741 acggcgatca tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat
     4801 ttttttccgt agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct
     4861 gcttttttct gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg
     4921 acttggcgga attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc
     4981 cagcttttga ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac
     5041 actggatcag ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt
     5101 ttcaagtcca gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt
     5161 ttgctgtaat ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat
     5221 tgcactgctt gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat
     5281 aactgaacag ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat
     5341 gcataatacg tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt
     5401 tgcgcattct tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac
     5461 agccattctg gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg
     5521 attataggtt gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt
     5581 tgataatatg caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc
     5641 agatcgtctg tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg
     5701 cctgtagctg gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct
     5761 tcatagaaat cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc
     5821 gtgtgcccat tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat
     5881 tcgaacaatc aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc
     5941 gatcccaact gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta
     6001 aaaaattagc cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc
     6061 tggtttttga agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt
     6121 ctccagcaaa atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg
     6181 aagagccaat cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg
     6241 aagaaaaaag gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt
     6301 ttttgaggta taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag
     6361 cagttctcct tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc
     6421 gcaagaaaaa tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac
     6481 cactaggact tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga
     6541 agaatggcaa tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa
     6601 aacgtatacg tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat
     6661 atggcaatca ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt
     6721 acaaagtaca cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg
     6781 gaacagtttc acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt
     6841 taaactatcg aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat
     6901 gacacgttga agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa
     6961 gtgttcggaa cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc
     7021 atcaatatcg aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca
     7081 cctgtcaatg attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt
     7141 gcgaaaacga tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg
     7201 aaacctcatg aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca
     7261 actcgagtac tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct
     7321 gtagataatt tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg
     7381 ggagatgaca tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt
     7441 tcagaatatt tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag
     7501 ggtggttatg tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg
     7561 aacacaggag caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa
     7621 gtatatgcct gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat
     7681 gcaccaatca aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca
     7741 gaaaacttaa caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat
     7801 gaaaatacac cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa
     7861 aaaagaggga aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt
     7921 tgtcacagct ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct
     7981 ttggtttgtt cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg
     8041 aagtgaaaac tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg
     8101 aagcgatgac ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg
     8161 gtcgccacgc gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag
     8221 caaaacctca cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct
     8281 tagaaacagc gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac
     8341 atcctgctgt tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc
     8401 tgcctgtaga tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag
     8461 atacgatctt ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca
     8521 aagaaatcgg tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag
     8581 cgtacggaaa ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg
     8641 ctcataaaat caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata
     8701 tccctgcatt gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt
     8761 tagcagggat ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag
     8821 cacgtaacaa caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg
     8881 aaatccctta tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc
     8941 gcttaattgg cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta
     9001 tttctactgg ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa
     9061 tgtatggaga agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa
     9121 ataccgaaga cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac
     9181 tagtgaagtc attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac
     9241 ttaactgaaa aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt
     9301 atcgaggtga aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc
     9361 tatcgtttgc atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca
     9421 aacggaggca attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga
     9481 ttcaaattaa aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca
     9541 gcaaatggat tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat
     9601 aaattttatt ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga
     9661 tctgtggata taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg
     9721 taaggacgac gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt
     9781 taatattttg ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt
     9841 tagggaaaaa ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta
     9901 tacaaaatga aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg
     9961 aattggaaga gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt
    10021 atggtcgtcc aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt
    10081 ttgagttttg ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga
    10141 ccgctagtgg atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg
    10201 attttcgcgc ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta
    10261 gtttttgtgt ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag
    10321 tcgattatgc cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga
    10381 atggtccaat cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc
    10441 gttcctgtca attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta
    10501 ttgatttttg tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt
    10561 tttaaagatg caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata
    10621 acgatcaatg gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg
    10681 atgacgctag attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac
    10741 gcagtcggaa tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga
    10801 atgaaaccta ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg
    10861 gttccaaacg atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt
    10921 gcaacgccat atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat
    10981 gttttaggtc gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga
    11041 ttattatttg caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc
    11101 ggaatcggga ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat
    11161 gataaagaat tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat
    11221 ggaatggagt tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg
    11281 gcaataagcc tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat
    11341 atagggcagt tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt
    11401 tgttgctcag ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt
    11461 gcgtctacgc ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca
    11521 tgaagattgc ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat
    11581 tgttgatggt cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg
    11641 tcagtaagcc taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc
    11701 cctttgatca agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt
    11761 gctttttcat attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct
    11821 tttattactg cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag
    11881 agccgcggag caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg
    11941 ataaagtaat tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat
    12001 ctgagcaact cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg
    12061 ataagaagca attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga
    12121 aaggctggcc ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg
    12181 attttttggt tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac
    12241 gacttgataa ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa
    12301 agttgcgacc ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg
    12361 acagacaaca gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta
    12421 acagcacttt tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg
    12481 gatgacacgg acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa
    12541 gtagctgctc aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat
    12601 cgtgtatttg aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg
    12661 atgtgcaaca aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc
    12721 gattatgtag ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac
    12781 atgcttcgtg gagtagacaa taataaagat caaacttact tccttagcca attatcacaa
    12841 gaacagcttt caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca
    12901 attgctgaac gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt
    12961 attggtgaaa agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat
    13021 atggtcactc ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt
    13081 ggtcaacgtc aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt
    13141 gtaggaaaag atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca
    13201 ttgtatgcca caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca
    13261 aaagaattca agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt
    13321 cgcttattgg atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact
    13381 cctggacaag cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac
    13441 cacgcatatc aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa
    13501 cagctatggc taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa
    13561 taaaattaat ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc
    13621 aataatatga ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc
    13681 ttttgcaagt gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga
    13741 cgggtattag caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg
    13801 tgtttgggat cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat
    13861 ggataatcgt tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa
    13921 agcgtccaga tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag
    13981 gtggcttcat cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg
    14041 caacgatgct gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg
    14101 ttccttctat tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg
    14161 gagttgaagg agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat
    14221 tcctattttt aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt
    14281 ggggtaacgt atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc
    14341 agattaaagg ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg
    14401 ttttagccaa ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc
    14461 tttgtttatt gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag
    14521 aagagttagc aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa
    14581 attggggagc agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt
    14641 cttggacgat gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga
    14701 aaggttgggg aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa
    14761 tcttgactaa tctattctta ttgactttct tagttactga ttatgcctat caatttgcct
    14821 attctttatg tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc
    14881 agttttcttt aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt
    14941 ttgaattgat gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct
    15001 atcttcctgg tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg
    15061 gaaaagaaaa agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc
    15121 ttgctgtagg ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt
    15181 gtagaagaat ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg
    15241 atcgaagcat tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt
    15301 ttcgaaaaac gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc
    15361 aaacaagaag tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat
    15421 ggagcgactg gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt
    15481 gtttcgatgc ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct
    15541 gttgactttg ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa
    15601 gccttagtca aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc
    15661 acattgctag atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca
    15721 tatgtattga tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc
    15781 gtagatgtct atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca
    15841 ggtattcgta ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat
    15901 cgagactaca cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg
    15961 aagaataaag aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt
    16021 ttaaaacaat gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct
    16081 acttctttca ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta
    16141 gaagaaacag gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga
    16201 ctaggttact gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc
    16261 ttgaaaaaac aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca
    16321 gtgtttttca tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact
    16381 cctgaagctt tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca
    16441 agcaagaagt tgaccaaaac aagaagagaa gtcgaaagaa agacgccgcg ataaccaaat
    16501 gcactggaaa cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat
    16561 tggttataac tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct
    16621 tggacagcag ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat
    16681 tgccaaacat tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca
    16741 atcgcaagta ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct
    16801 atcaaagtgg caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca
    16861 tgtccatgca gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat
    16921 gcttggatga tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct
    16981 ttgaaaatct gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg
    17041 acgaaaaaca gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta
    17101 gggcgatagc caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct
    17161 gtaacagaac cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca
    17221 gttgcgacaa gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc
    17281 aatgcgacta attgatacac actagtcacg caacccatca aagaaatgac gattgccatt
    17341 cctaatgaag cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac
    17401 caaggagaga taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag
    17461 ttcaattgtg acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg
    17521 actaaactaa atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt
    17581 tcccaagttt ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct
    17641 tctttctgaa aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa
    17701 tgaaagtctc ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt
    17761 tatgagagga aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg
    17821 ctttcgagaa ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa
    17881 acgcttggat atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa
    17941 aacagcgcta acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt
    18001 gggaaaaagt ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt
    18061 ttcaaatcaa gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt
    18121 attagatgga atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt
    18181 aacgaaagaa gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct
    18241 ttccttacgt tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta
    18301 tttttggatg atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa
    18361 caattttaag atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg
    18421 aaaagtgact aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta
    18481 attaccatgt tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt
    18541 gaagaagaag aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg
    18601 aacgaaatcc atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt
    18661 tggaatgacg gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag
    18721 ggacttgcgt tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat
    18781 gaaacaacta attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg
    18841 tgctaatcaa aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct
    18901 tggtcattta ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca
    18961 aatggacacg ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg
    19021 acatcaatta aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat
    19081 tgcgacagat gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca
    19141 tttcaataaa ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac
    19201 tggattcaag caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc
    19261 ccgggcaaaa gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta
    19321 cgcggataat ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca
    19381 agaaaagaaa atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg
    19441 aaaagaacaa gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc
    19501 acaaaaactt gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa
    19561 attaaaaacg gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa
    19621 tcaacgctat ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac
    19681 ccataagatc gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag
    19741 tacgatgaaa gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt
    19801 gaaaaacgga gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca
    19861 ccatgcactt ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg
    19921 aaaaatctat caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt
    19981 cagccaaaca aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg
    20041 tgtcgtccaa gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga
    20101 aaaaggaatg agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa
    20161 accattgtca gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc
    20221 tacgcgtgca gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca
    20281 aggactgcaa gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt
    20341 aacaccagat ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa
    20401 acaagcagga accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat
    20461 caaatcaagc aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg
    20521 tggggccaat ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc
    20581 agattgcaac taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg
    20641 tcaccggaaa atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga
    20701 tggaacgaca gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga
    20761 agaacgacgc ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc
    20821 agcagcacta aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt
    20881 ggtcagcttc aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat
    20941 ttcggcttat ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt
    21001 gacaagactt ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat
    21061 attattggtg aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa
    21121 caggacgctg tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac
    21181 ataacaattg gattccacca atgaacagga agatcgagac catggaaggc catcctgatg
    21241 taggatcacc aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga
    21301 cacaagacaa agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga
    21361 taccatcaat cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac
    21421 gttcgcggtt ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt
    21481 ttgaaaatcg gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca
    21541 agcggaaatc acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt
    21601 agaatttttt cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg
    21661 tgccgacaca atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg
    21721 gcggatcttg cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc
    21781 cagcgtacag agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag
    21841 gataagctga ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa
    21901 taaacaaata ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag
    21961 gaacagtttt ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc
    22021 tttcaatcgt atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc
    22081 tttaaaattt gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt
    22141 ttaccatgtt tcctcggttt atcaaacttt taagaacaat ttttattagt tggcaagtag
    22201 ttttggatag agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa
    22261 atacacacac ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta
    22321 tcttttcagc caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa
    22381 gaacagtttg aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata
    22441 aagagaggta cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga
    22501 gaaatccgaa cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg
    22561 attgggcagg caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc
    22621 gggcaaaatg gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa
    22681 cgccaattgt tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat
    22741 tgttccatgc gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa
    22801 taaatagtaa agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc
    22861 gtgtaccttc tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag
    22921 tgatctcaaa ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa
    22981 acaagctgtt gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt
    23041 ttcgtttttt ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga
    23101 atttttcaaa cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca
    23161 tggcgaattc gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag
    23221 attggagtgt accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta
    23281 atccaagaaa agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga
    23341 tctgtccttt ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag
    23401 aaccacaaac agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt
    23461 ttttaccaat caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa
    23521 ttccttttac accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa
    23581 cagagaattc taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt
    23641 taaaaaaagt atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact
    23701 gtggcagata aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac
    23761 cgggtaagat ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt
    23821 tcatttgatc ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact
    23881 aaaccagtat tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt
    23941 ttcgaataaa cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa
    24001 tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg
    24061 acctcttggt aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa
    24121 aaacatgaac agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc
    24181 acaacctatt gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta
    24241 tgttctttaa ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca
    24301 aacagacatt ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg
    24361 aagtaatgaa cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct
    24421 gcatagcttg gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa
    24481 tacaatactt gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag
    24541 tttttaagat aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga
    24601 cgtgtcggat gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag
    24661 aacgcatagc agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc
    24721 ccggcagtta cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt
    24781 gcttcaattg aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc
    24841 acttggaaga taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat
    24901 gaaacaaaat aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg
    24961 atgattggat catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa
    25021 atgaatatca taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga
    25081 tagtctttaa acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc
    25141 ggaataattt tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta
    25201 gtaccaagat gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag
    25261 acagctgatt gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt
    25321 ctattataga tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa
    25381 aagttatgga agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac
    25441 aagaaatttt tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca
    25501 aatcaaacag ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga
    25561 gatgcttcca actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga
    25621 atgggaagaa actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat
    25681 cgttgcttcc cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa
    25741 aaggtttcct aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca
    25801 agtagcgaaa catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt
    25861 acaaagatac tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt
    25921 gtggcttgta agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga
    25981 gatgaatatc caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct
    26041 gaaggaagga atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat
    26101 gactccgttg aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga
    26161 tcagctgcta gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca
    26221 cagcgaggag aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca
    26281 ctaaagataa gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag
    26341 ttaatttttc ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata
    26401 atcagcagta tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga
    26461 acgataatcg tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc
    26521 tgctctcatt atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg
    26581 tagggatgca agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt
    26641 tgaattccaa atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa
    26701 tgggactctt gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact
    26761 ggtttcgatt cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct
    26821 tgatgatggc actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat
    26881 cggacttttg gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca
    26941 tggcccaaga tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc
    27001 tgatgtctgg aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg
    27061 gagcaggaac gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg
    27121 gattcaaatc ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt
    27181 caggaatgag ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg
    27241 gtaaaggatt caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta
    27301 ccgttatcgg tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta
    27361 ttttaatcta tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata
    27421 tcgcaagcgg tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata
    27481 tcggattact tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga
    27541 ttttagggaa catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac
    27601 ctgcaagatc ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc
    27661 aacatgcaaa aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga
    27721 tatgatcaaa gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt
    27781 accaatccgt cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga
    27841 tcaagtaggc gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct
    27901 gatcaagcga ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa
    27961 aatttatgaa ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc
    28021 tttggatctt atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag
    28081 aacttggaga catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg
    28141 aacctctgat cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg
    28201 gtacagtttc ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag
    28261 atgaaatgaa tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat
    28321 tataaaatca gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca
    28381 taagcaatcc tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt
    28441 gaagagagcc ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag
    28501 cagagtaagc gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata
    28561 cggaaagaag ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca
    28621 aggtggtaac acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct
    28681 tttttgtttg aaaaattata agagaaagat gtgaatggca tgtcattaat ccaactaacg
    28741 aatgtcaaaa agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca
    28801 ctaaacgttg aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt
    28861 acattagtcc gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac
    28921 gatcaagata ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt
    28981 atgatcttcc agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg
    29041 ccgctggaat tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg
    29101 cgtttagtcg ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag
    29161 aaacaacgcg tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat
    29221 gaggctacta gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg
    29281 atcaataaga aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc
    29341 aaaatctgca acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta
    29401 ttgactgtct tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc
    29461 gaaccagaag ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc
    29521 cgtttggcaa ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg
    29581 attcgacgct ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa
    29641 ggttcctttg gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca
    29701 gaagtgttca tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt
    29761 ttcagaaaca tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct
    29821 tgatacacta tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt
    29881 gggattgatc ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg
    29941 aatcgtatcg atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt
    30001 gatcattcca tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc
    30061 agctcttgtt ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga
    30121 agtcagccct ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat
    30181 ctggaaggtc ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac
    30241 gatctcgatg atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg
    30301 tcttgcttgg caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt
    30361 aattatttta ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac
    30421 agacaaaaga taataggata tagggggatg taggatatga aaaagaaaat ttttggattt
    30481 gcagcagcgc ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa
    30541 gattcagcaa ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac
    30601 gcagagattt tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc
    30661 gtcaaattcg atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc
    30721 aattacttcc agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt
    30781 gtcaatgccg gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat
    30841 gtttccgaat taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga
    30901 cgcatcatca caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg
    30961 gaaacagcaa cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg
    31021 atcgacccat cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc
    31081 aacgcaaact tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa
    31141 aaggacaact ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac
    31201 aaaatcaaaa aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac
    31261 aaatggggcg gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa
    31321 agatgagaaa taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta
    31381 gaaatcaaaa ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg
    31441 cgcttcattc tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg
    31501 catgtctgtc ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa
    31561 aggcgtaaac ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac
    31621 aggaaaatct accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg
    31681 cgaaattttg tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact
    31741 tggcttattt ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt
    31801 catgcgtgca gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt
    31861 tttgaaaaag ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg
    31921 ttatctgaac gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt
    31981 gatgttagaa cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc
    32041 tttgaaagtc gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat
    32101 catcacccac tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat
    32161 ggaaggccga gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg
    32221 ctacgctggt atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg
    32281 agcaagaatg aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt
    32341 aagtaaaggc gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga
    32401 tgaactgcca ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt
    32461 gaaagaaaca caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga
    32521 caatcctgtg cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt
    32581 agcagaaaaa ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact
    32641 agtccaagaa tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact
    32701 ccatgtagct tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga
    32761 tgaaccactc gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca
    32821 tgtgttgatc gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac
    32881 caaagaacaa gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg
    32941 ttcaaaaatc aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa
    33001 tcgccgtggg cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga
    33061 cggtcatgtg atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt
    33121 caaaatcgtg gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa
    33181 taaagcccct catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac
    33241 attgaccttt aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca
    33301 agaaagtcgt gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt
    33361 gatcgatgaa aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga
    33421 agaaatgtac tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat
    33481 tcgaggattc ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt
    33541 agtcgaagta atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat
    33601 tttcctatct tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg
    33661 acgacccaaa agccaaaaca agtactccag gcattagcgt actattacga acacgataac
    33721 gccaatgttc atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa
    33781 gcaagagaaa aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact
    33841 cgtggtacga cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa
    33901 gcaggagacg agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag
    33961 cagttggcaa aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc
    34021 ctggatatgg aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct
    34081 catgtatcca atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat
    34141 gaaaacggcg gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat
    34201 gtccaagcac tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca
    34261 ggtattggtg ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt
    34321 ggtggcgaaa tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg
    34381 aaattcgaag cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat
    34441 tatttgcaaa aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat
    34501 gtgttgccaa aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat
    34561 tttcggacag gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca
    34621 gctttagaca tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg
    34681 aagtaccttg aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa
    34741 gacgccgacc gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact
    34801 atctaaatta gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca
    34861 tcgtggaagc ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga
    34921 tgtgatcgaa ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg
    34981 cagtggctgc tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa
    35041 atcaatagaa gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga
    35101 agtcaaagat cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc
    35161 cgctcggatc aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga
    35221 tggtaccgca gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag
    35281 aattagaaga atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag
    35341 aaggcttgac agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga
    35401 tgcttgaatt ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg
    35461 gccccgacct ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca
    35521 aacccgctcg tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg
    35581 gtataccaga agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag
    35641 tcgtgtacca taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg
    35701 actctgcttt gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc
    35761 caaccgacaa caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt
    35821 acgtgccaaa aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg
    35881 aaaacacggg gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact
    35941 atgtcgaagg ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag
    36001 aaatctttac aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca
    36061 atgtgtacaa cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga
    36121 tcgacggaaa cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa
    36181 aaggtgcacg aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata
    36241 cgggtgcaaa aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa
    36301 tttcaaaaga cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg
    36361 cgggttcgat ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg
    36421 atacgatccc attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag
    36481 tctcaaaaat ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg
    36541 aagcaactga aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg
    36601 aatatgcagt cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa
    36661 cctgttatag gagctagaac ccttgatata t
//
LOCUS       DAZKMB010000015        32347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000015 DAZKMB010000000
VERSION     DAZKMB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.015"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..1949
                     /locus_tag="ACG8V3_000688"
     CDS             51..1949
                     /locus_tag="ACG8V3_000688"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0299252.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(2279..2998)
                     /locus_tag="ACG8V3_000689"
     CDS             complement(2279..2998)
                     /locus_tag="ACG8V3_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0299253.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    3188..3235
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            3335..3667
                     /locus_tag="ACG8V3_000690"
     CDS             3335..3667
                     /locus_tag="ACG8V3_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299254.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3654..5657
                     /locus_tag="ACG8V3_000691"
     CDS             3654..5657
                     /locus_tag="ACG8V3_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0299255.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5676..6146
                     /locus_tag="ACG8V3_000692"
     CDS             5676..6146
                     /locus_tag="ACG8V3_000692"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0299256.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6167..6751
                     /locus_tag="ACG8V3_000693"
     CDS             6167..6751
                     /locus_tag="ACG8V3_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0299257.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6766..7752
                     /locus_tag="ACG8V3_000694"
     CDS             6766..7752
                     /locus_tag="ACG8V3_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0299258.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            7742..8053
                     /locus_tag="ACG8V3_000695"
     CDS             7742..8053
                     /locus_tag="ACG8V3_000695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0299259.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8075..9856
                     /locus_tag="ACG8V3_000696"
     CDS             8075..9856
                     /locus_tag="ACG8V3_000696"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0299260.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9849..11225
                     /locus_tag="ACG8V3_000697"
     CDS             9849..11225
                     /locus_tag="ACG8V3_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0299261.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11232..11867
                     /locus_tag="ACG8V3_000698"
     CDS             11232..11867
                     /locus_tag="ACG8V3_000698"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0299262.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11897..13252
                     /locus_tag="ACG8V3_000699"
     CDS             11897..13252
                     /locus_tag="ACG8V3_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0299263.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13444..14838)
                     /locus_tag="ACG8V3_000700"
     CDS             complement(13444..14838)
                     /locus_tag="ACG8V3_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0299264.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14930..15901
                     /locus_tag="ACG8V3_000701"
     CDS             14930..15901
                     /locus_tag="ACG8V3_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0299265.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15898..16680
                     /locus_tag="ACG8V3_000702"
     CDS             15898..16680
                     /locus_tag="ACG8V3_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0299266.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16849..17220)
                     /locus_tag="ACG8V3_000703"
     CDS             complement(16849..17220)
                     /locus_tag="ACG8V3_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0299267.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17354..17797
                     /locus_tag="ACG8V3_000704"
     CDS             17354..17797
                     /locus_tag="ACG8V3_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0299268.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17840..18649)
                     /gene="yidA"
                     /locus_tag="ACG8V3_000705"
     CDS             complement(17840..18649)
                     /gene="yidA"
                     /locus_tag="ACG8V3_000705"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0299269.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18668..20035)
                     /locus_tag="ACG8V3_000706"
     CDS             complement(18668..20035)
                     /locus_tag="ACG8V3_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0299270.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(20019..20849)
                     /locus_tag="ACG8V3_000707"
     CDS             complement(20019..20849)
                     /locus_tag="ACG8V3_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0299271.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            21012..21449
                     /locus_tag="ACG8V3_000708"
     CDS             21012..21449
                     /locus_tag="ACG8V3_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0299272.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21525..22145
                     /gene="rpoE"
                     /locus_tag="ACG8V3_000709"
     CDS             21525..22145
                     /gene="rpoE"
                     /locus_tag="ACG8V3_000709"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0299273.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22334..22840
                     /locus_tag="ACG8V3_000710"
     CDS             22334..22840
                     /locus_tag="ACG8V3_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0299274.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22857..23756
                     /locus_tag="ACG8V3_000711"
     CDS             22857..23756
                     /locus_tag="ACG8V3_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0299275.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23843..24727
                     /locus_tag="ACG8V3_000712"
     CDS             23843..24727
                     /locus_tag="ACG8V3_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0299276.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(24919..24990)
                     /locus_tag="ACG8V3_000713"
     tRNA            complement(24919..24990)
                     /locus_tag="ACG8V3_000713"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24955..24957),aa:Arg,seq:ccg)
     gene            25050..26384
                     /gene="rpoN"
                     /locus_tag="ACG8V3_000714"
     CDS             25050..26384
                     /gene="rpoN"
                     /locus_tag="ACG8V3_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0299277.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            26533..27624
                     /locus_tag="ACG8V3_000715"
     CDS             26533..27624
                     /locus_tag="ACG8V3_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0299278.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            27665..28666
                     /gene="gap"
                     /locus_tag="ACG8V3_000716"
     CDS             27665..28666
                     /gene="gap"
                     /locus_tag="ACG8V3_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0299279.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            28776..29966
                     /gene="pgk"
                     /locus_tag="ACG8V3_000717"
     CDS             28776..29966
                     /gene="pgk"
                     /locus_tag="ACG8V3_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0299280.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            30070..30825
                     /gene="tpiA"
                     /locus_tag="ACG8V3_000718"
     CDS             30070..30825
                     /gene="tpiA"
                     /locus_tag="ACG8V3_000718"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0299281.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            30941..32239
                     /gene="eno"
                     /locus_tag="ACG8V3_000719"
     CDS             30941..32239
                     /gene="eno"
                     /locus_tag="ACG8V3_000719"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0299282.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        10671 a         5707 c         6889 g         9080 t
ORIGIN      
        1 tgtataacaa ctttataata ttgagtatac caatttttgg aggcgtttta atggaccgtt
       61 atttagattt actttcagaa aaattcccct caacggagga tgtcatcacg gaaatcatca
      121 atctcgaagc aattttgcag cttccaaaag gaacagagtt atttttgagt gatatacatg
      181 gcgaatttcc tgcttttgat catattctgc gtatcggttc aggaaatctt aaagaaaaga
      241 tcaaagacct tttttctgaa caaatgacgg aaacggatat cagccaattc actattttta
      301 cggcttatcc agaatatgca ttaacgactg attggtacaa agaacaagat aaatcgcagc
      361 tgatccatca tttgatcgat cttttacggt ttacgacagt caaatatacc cggtcaaaag
      421 ttcgaaaagg attgccgaaa gaatatagct atatcattga ggaactcttg tatatcgatg
      481 accgtatcaa tggaaaaaaa gactatgtaa aaaaaatcat tgaacaactt gttttgatga
      541 aggaagaaga aaaattttta acgaaacttg cacagacgat ccaaaaatct gtcatcgatc
      601 atttgcatat cgttggagat atttttgacc gaggaactca agcagccaaa gtgatggatc
      661 gaatcatgga tttttcttct gtagatatcc aatggggaaa tcatgatatt ttgtggattg
      721 gtgcgtattg cggctctgag gcatgtttac tgacactttt acggattgca gcacgctata
      781 attatttatt tgaattagag aaagaatatg ggttgaattt acgtccattg ttttcttttg
      841 ctcatcaaac ttatcagaag aatccagtat ttgcgcctaa aaacagtaaa aatctatccg
      901 aaagagagca agaagaatta gaaaaaatcc atcaggcact ttctatcctt caattcaaat
      961 cagaacatca gttattggat cgtcgacctg aatttaaaat ggacgatcgt aaattgctgg
     1021 aaaaaatcga ctatgaagca tcaacgattg atttagaagg tcagctctat gagttgaaag
     1081 gaacttgttt tcaaacgatc cagccagatg atccaaaaaa attcttggaa gaggaacaaa
     1141 aagtgattga tagtttgatg tattcattcc agcactcttt gagaatgcaa aaacatatga
     1201 cgtttttgat cgaaaaaggg ggaatgtatc tcacgaacaa tcagcatgtg ctgtttcatg
     1261 gctgtatacc tgtagacgag aagggacagc tcttagcatt tgaattagac caatcttatc
     1321 gcggaaaaga actgctgggc ttttttgaaa aacagataac tgaaagcgca aaagatctga
     1381 ctgcaaaaga agatttagca accgatttga tttggtatgc gtggagtggg ccattttcgc
     1441 ctttattcgg caagagtaaa atggcgactt ttgaacgtta ttttctcaca gagcctcata
     1501 cgcacgtcga gaaaagcaat ccttattatc acttgcgaga tgaagagtgg ttcagtgaaa
     1561 aaattttagc agaattcgac acaaaagaag aagggtcagc aatcattaat gggcacacgc
     1621 cagtaaaagt aaaaaaaggg gagtcaccga tcaaagcaaa tggaaaagta tttgtcattg
     1681 acggaggttt atccaaagct tatcaaaaaa cgacaggaat tgccggttat tcactattat
     1741 gcaattctta tggtttccaa atcgtgacac attatccgtt tttgcctatt gaacaacaat
     1801 ttgcaaaaaa atccgaccaa acaaatgtga aaaaagtaat cgaacagcaa ctaccacgca
     1861 aactcaatcg tgatacaaca aaaggtatgg aactgcagca gcagattacc cagctcaaaa
     1921 ggctgttgga ctatcgaaag gacgattgag aagaattcca gtgaacattg ttccatagac
     1981 tggagactga gacaaaagtc gtgtcgcagt ctctttttcc ggataaacgg tgttcttctg
     2041 ccaactcctc ggtcacaagt cacactattc aacaaacatg aaaagctgtt ttttgaaatt
     2101 gctccttgtg actcagagtt gaacggcagg ctcacgacct cttggtataa acggtgttca
     2161 aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa tgtgaaaagc cattttcaca
     2221 aaatttttct taatactcga aactaaacag ctttttcaca accccctcag aaagtctatt
     2281 aaaattcatt cacaaagaaa tcgaataagc gctgttcaga tggatgtaca cggtgggtaa
     2341 gttccggatg ccattgaaca ccgaggattt tatgcatatc atctttgctt tgaaccgctt
     2401 caacgagtcc atcgttagaa aaagcgatgg cttttaataa aggtgaaaga tctttgactg
     2461 cctgatgatg gtaagaattc acaaaatatg gatcggggag aaggttgctt aacaaactgt
     2521 ctgaaacaat cttgacttcg tgagtcgtaa attgcggggc agtgggctgt tgttcatgtt
     2581 ttactgacca ctttggatat aatgaaagat cttgataaag tgtaccttcc aaaacgacat
     2641 tcaatagttg gagtccccgg cagactgcaa aaattggttt tctttgtttc aatgcttcca
     2701 agatcagtgc ctgttcaaat tggtctcgtt gaatatttgt ttccgttagc tttggatgag
     2761 gatcttccat gtaaagttgt ggactgacat cttgaccacc tgcaagaata agtttgtcga
     2821 tttgtccaat cagttgagga gctgaatctg gagacatgac tggcagaata agaggcaatc
     2881 cttgagcatc taaaactgca tccacgaatc cttgcggtgt atagctcact tgatttcctt
     2941 ggaaagtatc tgttgctcga atcagttgat ttccggcaat gccgatgatt ggtttcattt
     3001 gtattccccc taaagtagac ttattgtaac aaggatttaa aaaaaattaa actatgtaag
     3061 acttgcccct atacttatct gttcttgctg tgttactatc tgtaaagaga aatttcgtgt
     3121 gaaattcatg acgatagttg aaaatattgc ttaatttgtt ataataaaaa gtaagcagaa
     3181 ataaaaaggt tgggcgtaag cttcaaggat ctagttcttc aaggggtgag aaggacattt
     3241 tattgaaggg gcctgcctat actaagttat ctttagaggt acatactttc aatgaaaaaa
     3301 gaaagcatgt acctcttttt taaatcgaaa ggaagtgaag caagtgttag aagctttgga
     3361 acaaatccga aaagcagaag aagaaaatga aaagcgtgct caaacactgc ggcagcagct
     3421 gaagatctat gcaacagaaa aagagcagga attggaaacg atccaaacaa actatcgaca
     3481 acaactaaaa cagttggtgg aagaaaaaga acaatttgaa atacaagaat tagaaaaaga
     3541 aaaaatgaaa ttgaaagcag aagcagacaa ggtgcaagca actttgaaag atcaatatca
     3601 aaagcatcag agtcaagcga tcggtgcgat cattgaaagg gtgaaagaaa cttatggccg
     3661 tcactaagat ggaaaaagtg accctcattt cagataaaag aaatcaggag aggattttac
     3721 aagcagtaca aggaatccaa aacgttgaaa tccgggatct gtatcaaaat agaacaaaca
     3781 accagtgggt ggagaagtat tttccagata ccacgattat tgataaagaa gcaaaattaa
     3841 gcgctttagg taatcggttg aaagacatcc aagaagctat ccaattcata caacaccacg
     3901 gcgatagccg tcaaaagaaa ctccatttga aacgtcaaga actttcctta caagatttgg
     3961 aagccaatta ttccgaagaa aatttcataa ataaattgaa agaagtcctt gatttaaaag
     4021 aaaagtggga acaactaact gaagaacgtg atcgtttaga agaagaggaa gaatggcttc
     4081 ttcattggca acagctggat attgctccaa gtatgtatgc aagtaagacg accatttttg
     4141 agatggggac tgtgagtgca gcaaattttg aaagtttcaa ggaagaactg agtaagcttg
     4201 atgacgttta tatggaagaa atagattata cgccaaaaca agtttatctt tcgtatgttg
     4261 ctttaaaaaa atcagttgct gttatagaag aaattgctgg gaaatacggg ttcaataaag
     4321 aaacgtatcc ttatgaacaa aatcctaaag aaaaactgca agagaccaag cagcaattat
     4381 ccattgttca cgagcagcaa aagaagataa cgacagcgtt aggtcagtgc agtggctata
     4441 tccaagaatt tgaatgggta gaagaagtca cacttgccgt tgcagaacga gaaaaaataa
     4501 aagaacggtt tatccatgcg acttatctga tcgttttgca aggttgggta gatgcagaag
     4561 aaaaacagaa tttgctgcat gtgttaaacg aaaccgtatc agaagaagat atatacgtca
     4621 gtttcgaaac accaaactca gaagagattc agcaggaagt ccctactaaa ttgaaaaacc
     4681 atccgcttgt cgaacctttt gaattgctga ctgaaatgta cagtctgcca aaatatgaag
     4741 aagtagatcc aacaccttgg atgacaccat tttatcttgt cttcttcggg atgatggtcg
     4801 cagatgttgg ttatggtttg ctgatgctca ttgggacaat actagctcaa aaactacttg
     4861 ttctaccacg cgggatgaaa cgtttcgtaa aattctttga gattctctcg ataccatcga
     4921 tcatctgggg attgatctac agttcatttt tcggacagtc attaccaaaa gaactgtttg
     4981 gcatccgctt gcctttccca atcctatcta cgacggatga cgtcaatacg attttgattt
     5041 tatccgtgat tttcggtctg atacagattt tggttggatt atttgtggca gcaaaggaaa
     5101 atatcaggag aaaagattac ttgagtgctg tcagtgatgg atttgcctgg caagggatcc
     5161 taattgggat cgttattgcg ttggttggtg ccatgttgat aaaaaataca gcatttgttt
     5221 accttggcgc tgggctatcg atcattgctg ccttatgtat cttgatcgtt ccgattatcc
     5281 aaacgccgtc taaagtcaaa ggtgctgcca aaggtgcgta taatctttac ggactgaccg
     5341 gctatatcgg tgatttagtc agttatacac gtttgatggc attgggtatt tcaggcggaa
     5401 gtattggagc ggcattcaat atgctagttg ctttcatgcc gccagctgct cgattcagtg
     5461 tagggatcct tttgatcatc gctttacacg cactgaatat gtttttgaca ctgctaagtg
     5521 cctatgtcca tggcgcacga ttgcagtatg tcgaattttt tgggaagttt tatacaggag
     5581 gcggacgtgc ctttgatccg ctgaaaacgg cggaaaaata cgtcaacatc aatcacaaga
     5641 aaaaaaacaa taaataattg gaggattttt atataatgat ggattactta attagtcaaa
     5701 atggtggaat ggtatttgcg gtattagcga tggcaacagc gacgattttt tcagggatcg
     5761 gttcagctaa aggtgttgga atgacaggtg aggcagcagc agcattgaca acaagtcaac
     5821 ccgaaaaatt cgggcaggct ctgattctgc aattgcttcc aggcacgcaa ggtttgtatg
     5881 gattcgttat tgcctttttg atttttatca acttaggaaa tgacatgtca gttgtacaag
     5941 gactgaactt tctaggtgct tctctgccga ttgcttttac aggcttgttt tcaggtattg
     6001 cccaaggaaa agtcgcagcg gcaggtattc aaattttagc gaaaaaacct gaacatgcta
     6061 caaaaggaat catttttgct gcgatggttg aaacatacgc gattttaggt ttcgttattt
     6121 ccttcctatt agtgttgaat gcctaagcat aaaggaggaa aagcagatga acgccattga
     6181 aaaaatcatc tctcagatga acgaagcagc agagcaggaa agagcggcgt tagaacaaga
     6241 agagcgaatg aagatcgatc aaaactttga acaaaaacgt acgcaagttg aaacagaaca
     6301 tcaaaaacaa aaagaaaaac aaatcgaatt actcgaaaaa aaataccgtc agctaaggaa
     6361 tcgccagcaa gtagaagtac gacaagaaaa tttgaatgcg aagcaggaat ttttaagacg
     6421 attgtttgca gatgcggtaa ctgaaatgga aaattgggat gaatctgaac aaatccagtt
     6481 tatcaaaaat gcattgtatt cattaccttt gacaggaaaa gtagcattca ttgctggaga
     6541 aaaatcagca gcctatcttt ctcagacgtt gttggatgaa tggaacaatg aacttccctt
     6601 tatgatggtt ttaagtgatg aaactgttgc tgatcaagcg ggttttctga taaacgatca
     6661 aggagttcag tataattttc ttttcagcag ccttgtacaa gatatccaag gaacgatgag
     6721 ttttgagatt gccaatcaat tattcgaata aggagggatc agtgtatgga ataccacgaa
     6781 ctaaatccct tgattcgcgg aagagagctt gaacttttgt ccaatgagac atttgaacgg
     6841 atgattcaaa caaattctgt cgaagcactt ggtgaaatat tgaaatccac gatttacagt
     6901 ccatatattt atgacggata tgaatatgac ttcgaacaga atcttttaaa ggagcagagc
     6961 cgtctatttc gctggttaaa ggaatcagca ccggagcctg aaatcgtttg gatctatacg
     7021 atgcgttata ctttccataa tctgaaggtt ttgacaaaag ctgagatgac aaatcaaaat
     7081 ctggaccatt tgtatatcga cgatggattt tattcattag aaaccttgaa ggatgccatt
     7141 catacgcaag tttcaaatga acttcctgaa agcattatgg acagtatacg agaagtacgt
     7201 gattattttg aagagtctac cattcttcag ggaattgacg tgatttatga tcgacagttt
     7261 ttgacagagc agcgacggct tgccgataag ctaggatatc aagaattgct gacagaagtc
     7321 attgctttta ttgacttgac caatatcaca acgatggctc gaggaatatt gcagaaaaga
     7381 acatatgggt tcatgacgac ggtactttcc agctcgggaa gtatcgaaaa agaagtattt
     7441 ttgaactttg tagataaaga tatggaagat tacacgaatt ttctattaga gacagattat
     7501 gctgattttc tgcgccctgt catccgtgag ggaaatattg atctcgtaaa gcttgaacag
     7561 ttgaaagatg attatctaag ttcattgtat caagaagcac aaacccaggc tttcggacca
     7621 ctgccactac tagccttttt gaacgcaaaa gaggtagaaa gcaaaaacct tcgtttactg
     7681 gttatcggca aaagaagcca tttcgaatca gaaacaatca gaaagcgggt gagacaagtc
     7741 tatgacgcat aagatcggtg tagttggcga caaagattct gttttgccat tcaaattgtt
     7801 tggctttgat gttcgttatg gtacaaccaa gcaaaccgtt cgtcaggcaa ttgaagaaat
     7861 ggcaaagaat gagtatggcg tgatttatgt cacagagcaa tgtgcagcat tgagcccaga
     7921 aacgatcgaa cgttacaagg aacaaatgat acctgctatc gtattgattc ccaatcatca
     7981 aggatcatta gggatagggc tggaagaaat tcaaaaaaac gtagaaaaag cagtaggtca
     8041 aaatatctta tagtatgaaa aggagataga aattttgcaa attggaaaaa tcataaaagt
     8101 ctccggacct cttgtaatgg ctgagaacat gtctgatgcc agtatccaag acatgtgtct
     8161 agtgggagat ctaggagtta tcggagaaat tatcgagatg cgtggcgatg tggcgtctat
     8221 tcaagtatat gaagagactt ctggaattgg tccaggcgaa ccggttcgct ctactggtga
     8281 agctttatct gttgaattag gaccagggat catttcacaa atgtttgacg gaatccaacg
     8341 gccattagat acctttatgg aaatcacaca aagtaatttc ttagggcgcg gagttcaatt
     8401 gcccgcattg gaccatgaga aaaaatggtg gttcgaacca acagtagaag ccggagaaac
     8461 ggtgagtgct ggagatgtta tcgggatcgt agaagaaaca aaagttataa aacataaaat
     8521 catggtgcca aatgggataa aaggaacgat caaaacaatc gaaaaaggtt cattcactat
     8581 tgatgaaaca gtatgtgtca ttgaaaccga aaatggtgac aaaaatttga cgatgcttca
     8641 aaaatggcca gtaagacgta gtcggccaat caaagaaaaa ttaaacccgg atgcacccat
     8701 gatcactgga cagcgagtga ttgatacatt cttcccagtg acaaaaggag gagcagcagc
     8761 tgtacctggt ccttttggtg cagggaaaac agtcgttcag catcaaattg caaaatgggc
     8821 cgatgtggac atggttgtct atgtgggatg tggcgagcgt ggaaatgaga tgacggatgt
     8881 tttaaatgaa ttccctgaat tgattgaccc taatacagga gaatcgttga tggaacgtac
     8941 ggtattgatt gctaatacat caaacatgcc agtagcagct cgagaagctt ctatctatac
     9001 gggaatcaca attgcggaat atttccgtga catgggttat gacgttgcta tcatggcaga
     9061 ctccacttca cgatgggcag aagcacttcg tgagatgagc ggacgtttgg aagaaatgcc
     9121 tggtgatgaa ggttatccag cttatcttgg ttcacggctt gcggaatact atgaacgatc
     9181 tggaagagtc attgcattag gatcagaagg tcgcgaagga agtataaccg caatcagtgc
     9241 tgtatccccg tctggagggg atatttcgga accagtcaca caaaatacat tgcgggtcgt
     9301 aaaagtattc tggggtcttg attccagttt agcccaaaaa agacattttc cttcgatcga
     9361 ttggatacaa agctattctc tttattcaac agaagtaggg aagtatatgg atcatatcct
     9421 tcaagctgac tgggcatcca tggtcaccga gggcatgcgt attttgcagg aagaacagca
     9481 gctgaatgaa atcgtgcgtt tggtagggat tgattccctt tcagataatg accgtctgac
     9541 attagaagtg gcaaaatcca ttcgtgaaga ttatttgcag caaaatgctt ttgatgatgt
     9601 cgatacattc acttcaagag agaaacaatt caaaatgttg aagatgatct tgacctttgg
     9661 tgaagaagga cgccgtgcgt tgtcgttagg tgcttattta agtgagatca tggacggtac
     9721 cgtatcgatt cgtgaaagga tcggacgaag caaatatatc ccggaaacag aattagaaaa
     9781 actagatcaa attattggtg aaatcaagca aacgatgcaa acgatcgttt cagaaggagg
     9841 catgagcaat gattaaagaa tatcgtacga tcggtgaagt cgtcggtcca ttgatggcgg
     9901 ttgaaaaagt tgctggtgtc aaatatgaag aattaatcga agtccgtatg caaaatggcg
     9961 agattcgtcg tggacaagta ttggaagtcc aagaagataa agcaatggtc caaatattcg
    10021 aaggaacaag tggtatcaat ttacgtgact cttctgtacg atttttaggc catcctttag
    10081 aactaggtgt atcggaagac atgatcggtc gagtattcga tggtttggga cgaccgaaag
    10141 ataacggacc agagatcctg cctgaaaagc atttggatat taacggagaa gtaatcaatc
    10201 cagtagctcg ggattatcca gatgagttta tccaaacagg tatctctgca atcgatcatt
    10261 tgaatacatt agttcgtggg caaaaattac cagtattttc tggctctggt cttcctcata
    10321 aagaattagc tgctcagatc gcgcgacaag caacggtact cgattcatca gatgatttcg
    10381 ccgtagtctt tgctgctatt ggaatcactt ttgaagaagc tgaatttttc atggaagatt
    10441 ttcgtcaaac gggcgcaatc gaccgttctg tcatgtttat gaatttagca aacgaccctg
    10501 caatcgaacg tatcgctact cctcgtatgg ctttgactgc tgcagaatat cttgcttatg
    10561 aaaagggaat gcatgttttg gttatcatga cagacatgac caactatgca gaggcattgc
    10621 gagagatttc tgcagcacgg cgagaagttc caggaagaag aggatatcct gggtatcttt
    10681 acacgaactt agctacttta ttcgaacgag caggaagaat ccgcggacta aaaggatcgg
    10741 ttactcaaat tccgattcta acaatgccag aagacgataa aactcatcca atccctgact
    10801 tgactggtta tattacagaa ggacagatca tcttgactag agagctttac aagagtggga
    10861 ttcagccacc aattgatgta ctcccgtctc tttcgcggtt aaaagataag ggaactggac
    10921 ccggaaagac aagagaagac catgcagcta cgatgaatca gctttttgca gcctatgcac
    10981 aaggaaaaca ggcaaaagaa ttagccgtag ttttaggtga atctgcttta tctgatgtag
    11041 acaagatcta tgcgaagttt gctgagcggt ttgaaaaaga atatgtcaat caaggattct
    11101 acacgaaccg ttcgattact gaaacacttg atctagggtg ggaactgcta tcgatgttgc
    11161 caagaaccga attaaaacga atcaaagacg acatgctgga caaatatctg ccagaaggga
    11221 agtgagttct gatggcacga ttgaatgtga atccgacccg catggaattg actcgattga
    11281 aaaaacagtt gacaactgcc acaagagggc ataaattatt aaaagataag caagatgaac
    11341 tgatgcgtca attcatcctg ttgattcgaa agaacaacca gctgcgtcaa gaaatggaag
    11401 agtctatgac agaagcaatg tctgattttg tattggcaaa tgcttcgatc acggaacctt
    11461 tcatcgaaga attatttgtt ttgccggcgg aaaacgttga tttgtctatc gtagacaaaa
    11521 atatcatgag tgtaaaagta cctgtgatga attttgaata tgatgaacag ctgacagaca
    11581 cacctttgga atacgggtat ttgaattcca gtgccgaact ggatcggtca atcgatcggt
    11641 ttactaccct gcttccaaaa cttttggaac tgacagaaat cgaaaaaact tgtcaactga
    11701 tggcgagtga aatcgaaaag acacgtcggc gagtcaatgc attagaatat atgacgatcc
    11761 ctcaattaga agaaacgatc tattatattc gtatgaagct ggaagaaaac gagcgcgcag
    11821 aagtaacaag gttgatcaaa gtaaaaaata tgggtaccac tgactaagtg gtatcttttt
    11881 ttaaaagagg aaaacgatga ttaaacaaag caaacaaata aagaaacgtt tgccggctgt
    11941 tcagcttctc gcattaggat tttttctcct gattttagtt ggcggcggcc tacttaccct
    12001 accatttttt agtaatagtg ggcatgggac aaattttatc gatgcgcttt tcactgcaac
    12061 ttctgctgtc tgtgtaacgg gattgacaac attgaataca gcagagcatt ggaacagtgc
    12121 tggtcaattt ttgatcatgc ttttgattga gatcggcggt cttggtttta tgatggttcc
    12181 gattctatgc tttgcattga taaagaagaa agtcagtctc agcacacgta ttatattaaa
    12241 agaagcgctg aatctagaag aagtatcagg tgtgatgaag ctcatgcttt acatcctaaa
    12301 attggccttt gtgatccaat tgattggtac ggttgcttta tcttttgtat ttgtgcctga
    12361 atttggctgg ttgaaaggta tctggtacag tttgttccat gcaatctcta gtttttgtaa
    12421 tgcagggttt gatttgttag gagacagttt agccaatcac cagcaaaatg tttatttgat
    12481 catggtggta tctgctttga tcatttctgg tgggttaggc ttcatcgtat ggcgtgattt
    12541 actggaatat catcagaaaa agaagatgac gattcattca aaaatcgcat taaccgttac
    12601 aggtaccctt ttattaggcg gattcgttgt gttctttttc acagagcgca atggggcaac
    12661 gctagtggaa gggaatatgt ttcaacgatt ggcaaacacc ttttttatga gtgtgacacc
    12721 tagaacggct ggatattatt cagttgatta cctccatatg agccatgcag gtctgatttt
    12781 aacgatgttt ttgatgtaca tcggcggaac atctgggtcg acggccggcg gattgaaaac
    12841 aactacgtta ggtgtgttgc tgatccagat gcgttcgatg ttgaaaggac gtacgagagc
    12901 ggaagcattt ggccgtacca tccgacaagg cgcagtgttg cgggcactta ctttattttt
    12961 tgttacgttg acgttgtgtg tagtcgccat catgatttta tcggtgactg aaacgatacc
    13021 taaaacttct ggcatcgaat atattgcttt tgaggtcttc tctgcgtttg gtacagtagg
    13081 tcttacgatg ggattgacgc cagacttgac tgcaataggg aaattgatca ttatagcgct
    13141 tatgtacatt ggacgagttg gtgtattgac tgtggtcttc tctctgatga caaaagcaca
    13201 aagaacacaa tcaaaataca aatatccaga agaaagtgtc atgattggat aatttcatct
    13261 tctgacagaa acctagtgat ttagtaaaat aagaaaataa aataattttt cttgcttctt
    13321 ggatacttta agtgcaaaat gttcgtttat tttttgaggc actacccgtt gttgaacctc
    13381 gggtagtgcc tcttttttat tgaaaggaat caataagatt tgattccttt atttatcaat
    13441 aggttataat tcatattttt ttattttttc ttttacgata cttagtccag aagctaaaat
    13501 ttcaaggtta ttagaggata gggaaattcg aacgaatgca tctggttgcg cacgtgtagt
    13561 aaagcgtctg gaatgataaa gtcggatacc gttagcaaga aagtcattct caattatctt
    13621 agggtcaatc tcaggatcaa ctggaagaat tcgatagtaa gggacacgat tcgttggttt
    13681 ttctactttg aaaagagaat caaaaagctg attggctctt tttgtaagac ttaatttagt
    13741 ttctaagatc ctatttgctt catttgaatg gaggatttga gtgatgactt ccacatctaa
    13801 tccagaagtt ttcacattta tattaaaaat tgcttgttct atcttgcttt ggacatgtac
    13861 tggaactaca agataagcaa ctcgcaatcc agaacaaata aattttgtca ttccagctaa
    13921 gtagattgtt tggttaggta ataaattttg gaaaggagga atgatcgtat gttgatagta
    13981 agttgttaaa aatgaatgga tatcatcttc aataacccat agaccttctt tttcaatcac
    14041 ttcggctaat tgctctcgtc ttttgtgaga gatttgaaac cctataggat tattacaaga
    14101 aggcataaga aagacaccat gaatcttctt ttttctacat tcctgaatca atatatcagg
    14161 tcgcattcct tgctcgtcaa aatcaattgg aacgatctct aaatgatata actgagccaa
    14221 ttcaatgaaa ttagcgtatg tatatcggtc tgttgctatt ctatcacctg gagagaaaag
    14281 tgcagaaagt gtgatagcca atccattttg aacaccggaa acaatagata gagattctaa
    14341 ggacggaatg actccttgag tttttaacca atttgctgca gtagtcaatt gaaaatttgt
    14401 gcctttggga tcttggtaat taagcaaacc aacaagattt gggttattac tagttgattg
    14461 aataaacggg acaacgaatt tattacattc ttcaaaagaa ctaaccagac caaaatcaat
    14521 gatatgttcg gttacttcat tggtagagat ggtgatggac tcaaagacat tgggagaaat
    14581 aaatgtgcca cttccaatat tcgtatacag caaacctttc tcaattccat atttataagc
    14641 cttgccaacg gtagtaaaat ttaaatctaa ataatcagct agctctcttt gggagggtaa
    14701 ctgagtgttt ttctgtaatt tattctttaa aatatcttgt tctaattgtt gaatcaacga
    14761 tcgataataa ggacgcttca atttttgttt gtctggtcgc caagttaacg ggtaattgtc
    14821 ataagaatta ataggcattt tctccactcc ttaaaattgt ataccataca attttaccat
    14881 tgattgtatt gattttagaa gttaatcttt tgtgaggagg gaattgtcta tgaaaattat
    14941 taattttgag gaagtagtag cttcttttag ttttgaagaa gcgattcaag taatgaaaaa
    15001 atgttttttt gattatgaaa gtggtaaaat cagccaaaat cctcgattgg tggaaatttt
    15061 acctgacgga aaaggcaaaa atatgtttgc gatgatgcct gcttatttgg gggagaacag
    15121 atattttggc acaaaagtca ttaccgcttt tccggataat catcagtatc atttaccttc
    15181 tcatttagga gagattttct tgtttgatgc tcaaaacgga ctacccaaag cattgataaa
    15241 cgcaaatgca gtcacctgga ttcgaacagc tgctgtatct gcgctggcaa cagattactt
    15301 agcaaatccg catgcagaaa cacttgcttt aattggagct ggtcaacaag cattgtcgca
    15361 tttgactgcg atttctacca ttcgtcccat cactacagtg tttgtttacg atattttaga
    15421 agaaagacga aatactttta tcgctaaagc taaaaaacag tatcctaaaa taacgtttat
    15481 aaatgcaata tcagtagaag atgcagtacg tccagcggaa atcatttgta cgttgacctc
    15541 tagtaaagag gcatttcttc aaaaagagtg ggtaagggaa gacgcacata tcaatgctgt
    15601 gggcacattt actcctgata caagagagat aagtacatcg ttgatggaaa cttctcaaat
    15661 ctatgtagat gattactcag ctgccttaac agaaagcggg gacctgctta tagccatggc
    15721 agaaggagat ttagaggaat cttgtattgt tggtacatta ggcgaattag tttctgagaa
    15781 aaaaacattt tataaaggag aatcgaggcc gacgattttc gacgcagttg gtctagctat
    15841 tgaagatctt tgttgtgcag aatatattta taacaaatta gagaaagggg ctttaaaatg
    15901 aacttagatt attacgtagt tgatgcattt gcagatgagg tgttcaaagg aaatccagca
    15961 gcggtttatg tgctagaaga atggctgcca gaaggtacga tgcaaaaaat tgccattgaa
    16021 aacaatttat ctgaaacagc atttacagtg aagaaaaacc aagaatttga gcttagatgg
    16081 tttacgccag atcgagaaat cgatctttgt ggtcacgcga cattagcaac agcatttgtt
    16141 ttatttaact attacaaaat acctgatgaa acgattaaat tttctactca aagcggtaat
    16201 ctatttgtga caagaattaa agattattat tacatggatt ttcctagtat tatgccgaaa
    16261 aaagtgccga tattgtctga atatgaagaa gcgattgggg ctaagattaa agaagcctac
    16321 ttagcacgtg atttattttt cgtattagaa gatgaaaaaa cggtggctaa attgcagcca
    16381 gattttacag ctatcaaaaa ttttgaatta ggaataggag tcatcgtgac agcagaaagt
    16441 gtgcaaaaag actttgtttc tagaaccttc tttccaaaat taacaatcaa tgaagatcca
    16501 gtatgtggat cagcacattc taatcttatt ccttattggg cagaaagaat gaataaaaca
    16561 aaattgtcag cttatcaaat gtcatcaagg ggcggggatc tagaatgtga attaaaagaa
    16621 gatcgtgtga taataggagg aaaagcagtt ttgttttcta aaggtacctc gtttatttga
    16681 tttctttcaa atgaattaaa aataaattgg tggagagtgg caaccttata gctacaatgg
    16741 ttgttaaaaa aagacgcaaa ctttttcaat ttaatgaaaa gtttgcgtct cattttaatt
    16801 attggtgttt gttttttatt caatcaagag taaaatgccc catactaact atcctcggat
    16861 gacttcgatt ttataaccat cagggtcaat gacaaagtag taagaaggcg cagtgccagg
    16921 taatcctttt aagtcagtaa tctcaagacc agcagcttta tgttgctcgt gtaatttttc
    16981 tagatcatca gctgctatag cgatatgacc atagccgtct cctagatcat acgcaccatg
    17041 atcatagttg taagtcaact ctaattcata gtcatcccca ggaagtgtca aataaacaag
    17101 tgtaaatttg ttttctggaa aatcacggcg gcggctttct tcaaaaccaa atgccttttg
    17161 ataaaattcg attgatgctt caagatcttt cactcgaaca catgtgtgag ccattttcat
    17221 agattcatct ctccttttcc ttttactcaa tcataacaaa aagaagtaag gaaatagttg
    17281 atttgtgctt gttcatgaga aatttctcat gtaaaatgga tagaaaataa aactgttaag
    17341 gtggaaacta ttcatggata aaccaatttt tggtgaaaaa aagcaatttg ctgattatca
    17401 aacccgttat gcagcatata ttgtcataga aagagatcag cagattgcag taatcgaggc
    17461 tccaaatggt gctttttttc ttcctggcgg ggaaattgaa ggagaagaga cgaaagaaga
    17521 agcgattgtc cgtgagctga tagaagaaat gggaattgtg gcagaaattt cttcttatct
    17581 gggagaagct gatgagtatt tttactcgaa ttatcgtgca acttattatt ataatccagg
    17641 atatttcttt gttgctgatt cttggaagcg tataggagaa ccaactgaaa agaccaataa
    17701 aatttggtgg gtgagccctc aagaagcgct tgtgaaattg aaacgtggta gccaccgatg
    17761 ggcagtcgag agatggatcg aaaaaaataa aaactgaaag aaagcgagta gatcagcttt
    17821 ctctcagttt ttttatttat tacagtacat attttttgat tgcttcagct acaccgtgct
    17881 cgtcgtttga tgaagtgatg acatttgctg cttctttgac aagagaaacg gcatttccca
    17941 ttgctactcc tagaccagca tattcaatca tggataagtc gttctcgtta tctccgatag
    18001 ccatgatttc atcttgatca ataccaaggt gctgtgcgag attcgcaact gccgcacctt
    18061 tacttgcttc tttatttagt acttcgaagt aatagggcgc acttttgacc gttgtgtatt
    18121 tatcacggaa agtttgcggt aaacgcgcaa ttgctgcatc taaaatttct ggttcgtcga
    18181 tcatcatcat tttgacgatg ttcatgtctg gtgtcatttc ttcaggtgtt cgatatttta
    18241 gcggcatctt gactaaactt gcttcgtgga tcgtataagc gctgatatct cgattaggcg
    18301 tgtagatcgc tttgtcatcg atcgaatgga gatgactgcc gacacggcgt gccattactt
    18361 cgatctctag gaaatcttca tgtgtcagtc cgtgacgggc gatgatttta cctgatttcg
    18421 tttgttgtac gagtgccccg ttataagtaa taacgtaatc attatcttga aaaaggttca
    18481 actcgtttaa ctgttcctgg acacctggta agggacggcc agtgcataaa acgatcgaaa
    18541 caccttttgc aacagcagcg gcaatcgctt cttttacttc ttttgttact tctcttttct
    18601 catttaataa agtaccatcg atgtcgatag cgactagttt gatagacata atttcctcca
    18661 atatatttta atttatttca atcaatgcgc cattacggat atggctggca aattcttgat
    18721 aagtttcgtc aaataaatca taatgatcac gcaaggacgg atcaaccatt tcttttggaa
    18781 agaaaaatcg ctcatctcct tgttcttgtc ccgctaatgc agcaacaagc tgactaacct
    18841 gtgataattc tatcaaagtt ccgtcatttc gcaaaatctc gatttgtgtg cgatgtctgc
    18901 cttgttctgg gcgataaaaa tcataaggta gatcgtagct agaattcaca gcagtgtaat
    18961 aaacaggatt atatccgacc ttttctacta acaaagtgag ttcttggact aattttgaat
    19021 cccggttgcc agaaaaagta gcagatttca gtggtttacg attcaaaaaa cgtttagcta
    19081 aatcatttaa aataggatca gcagattcag accattgagt aaagtaagtg cccaagaccc
    19141 catcatcaag tttgaggtat tcttctaaag taaagcttcc gtcaagaaat ggcagaagta
    19201 attgagaatg gagttcaaaa ctgctggcat cttcttgata caactcgtgt gcgcgatgaa
    19261 gcagatgctc caaaattact tccataccac gggaaacagg atggaagtac acttgcacat
    19321 acatttgata tcggctaacg atataatctt ccactgcatg catgccactc atggaaaaag
    19381 cgatcccgcc ttcataagga cgaatcacac gtaatattcg ggtaagatca aatgttccat
    19441 attctgttcc agtgaaataa gcatcccgaa gcaaatagtc cattcgatct gcatcgatct
    19501 gactagaaat catttggaca acttgtgggt tggggtaagt tttttggatc acactagcta
    19561 ccttttcagg aaaaccttct tctactcggt taagaatctg atacacttct gtttctggag
    19621 aagtgatgat ctctacagtg atggcttcat gattcgtatg gaagatatgt tcaaatgtat
    19681 gagagtaagg accatgaccg acatcatgca acagtgccgc acataaagtc accagccgtt
    19741 cctgatcatc ccagcctccg ttgcctattt tttcttttga aaaatttcga gaaaagatat
    19801 cgcagattcg tcttgaaatc tcataaacgc ctaatgaatg agtaaaacgg ctatgttctg
    19861 ctccgtgaaa agtgaaagaa gaagtgccta attgtttaat gcgtcgtaga cgttggactt
    19921 ctttcgaatt gatcaaatct aagattactt gatgctggac atggatatag ttatggactg
    19981 gatcacgaaa aactttttcc ataggcagca tttgatattt ataaggggtt gtcataatgt
    20041 agctccttta tgttattacg ttgttccatt ctctggattt ggttgttcca ttgctccagc
    20101 tggatttctt tttttagcca gtcggtagaa tcctgtaaat cacttgcaga atacagcttt
    20161 tcaaaagctt ttgcaaatgc ttctttgact tcttttacaa aaaggggttg gtcaagcagt
    20221 tcttcaagtg ttgccattgt agcaggatcc actgcaggat agccatttgt accaaagtct
    20281 tcttttaaag cagattgata aaatcgcttc atgacttgcc cacgtttttc ctgattaccg
    20341 ttgacactta agtacatcat aacagaaatc ccatttttga tccgacgctg agcgataccg
    20401 gcaaattttt tgccatggat gcttaaatca taagttccag gacaataaga atgggtgatt
    20461 tcgaatgctt gtatcggtgc ctttggaaaa gcgtatttag tcaataggag catttgctcg
    20521 taagcgtcat ctatggttat tttttttgtg tcactttggg ggaaaatcaa agaaatattc
    20581 aaaattcctt catctgatac gatccctaat ccacctgaat ttctgatcac tggagcgtaa
    20641 cctgcatctt ccagtgtttg gatcccgtca cttagaaaag gagttcgaac atccttcata
    20701 cctaagatga ctgtgcagtc atattgccag aaatgtaaaa agatttgctg gtgttcttta
    20761 gaatattccg ttaatatatc tgttaatgca aaaggggaaa agtcgttttt ttgataaatg
    20821 tgctgatcta gtcgaatcgc agggttcatt caaccggctc ctttgtataa attcatattt
    20881 tctacaatat tatactctaa aatcaaagag ttgttttgga aaaaaggttt tctgtaaaaa
    20941 tggacaagtt ggttgacaaa acaaaagaaa ctaggaacaa tgtagctatg agaagtaaag
    21001 gagaatgaat aatggaatta tcacaaggaa caccgatttc aatcaaattg cgtacaaaag
    21061 taaaacagca aggggaagtg caagattttt attttgattt gaaagggcaa atggtgacaa
    21121 ttggcgatac gctgtacata cgttataaag agattcagga ggagacgggc gatgagattc
    21181 ctgtaacgat caaattagtt ccagatggtc atgtacagct gatccgtgca ggagaaatgc
    21241 ggatgcgttt gaaatttggc tataaagaac ggttagaaac atcctatcga acaccatacg
    21301 gtatgatcca gattgctact tttacgaaag aactgcatgt tagtttgaaa gaccgtccca
    21361 cagcagggaa ggttcgaatc gattatgatc tgttcatggg accggaaaaa atcggcgaat
    21421 attatttgac cttggatttt acagcgtaaa tataaaagaa aagtttgatt ttttgtgtct
    21481 gattttgtat gataaggagt agactgtgaa aggacgtgtc ctgattggaa attagtgtat
    21541 ttgaaggtgc aaacaaaagc gaattatcaa tgatcgaagt agcacacgcg atcttagaac
    21601 aacgtggcga tgtcatggat ttttctgatt tagctaacca gatccaaaac tatcttggga
    21661 aatcagacag tgaaattcgt gattcactcg cacaatttta tactgacttg aatattgacg
    21721 gcagctttat ctcattaggg gataatcgtt ggggcttgcg ttcatggtat gctatcgatt
    21781 cgatcgatga agaagtaaac catggaatgg acgaagagga tgaagatact ccacgtcgcc
    21841 gtaaacgcaa gaaagtcaac gcctttatca atgatgatga agacgcgatc gattacaatg
    21901 acgatgaccc agaagataca gatctgacag aagaagatga cgatgatctg tttgatgacg
    21961 atgatgatga agatgaagaa atcgctgctt ataattctga cttgcaagaa atcggagcag
    22021 atgacccgac agatgatgaa gaagacttgc caggaaatat cgaagaagat ttaagtatca
    22081 tagacgaaga cgatgatgat gaagattatg aagaggaaga attttctgac tctgaaaaag
    22141 aataaacatt attttcttac gagaggttgt gacagagacg atcagcctat cagaataagg
    22201 aggtgtgaca cgatttttgt cacaccttct tttttgtata taaaaatccc aagtaaatac
    22261 ataaaaaata aaaaaattga aacttttagc gcaccttgta cgtcatatta agtgaggcag
    22321 aaaggagtgg agaatgattt ttactggata taagaaaaaa cggcagatcg aaaaataccg
    22381 tcacctgctg aatggactca tcgaaactga ttatcaaaaa atgttccgta tcacgatgaa
    22441 ttacgtacat gataaagaag aggccttaga tgtgatgcaa gatagttttc ataaagccct
    22501 gaactatttt gaaaaagaaa aagagatcga gcattttagt gcatggtttt atcggatatt
    22561 gatccgcacg gcattagata gttggcgcag acaaaaaagg acgcctgtgg acttatttga
    22621 aattcaagat atcccccaac agttaccttt agaaacttcc gttgcagagc tccatatgat
    22681 tttagataaa atagacagtc cggaaaaaga gattctgatc ctctactttt ttgaaggatt
    22741 tcgtttgaaa gaaattgcga ctatcttgga tatgaatgaa aacacagtta aaacgaaaat
    22801 gtaccgctca ttgaatgctt tacgtcaaat cctagaatga aggagagaag agtgccatgt
    22861 attcccaaaa agaaaattta aaaaaactaa agaaaactta taaagaacaa gcaattccca
    22921 aagggcttaa agaggagatc cgccagcgtt ttattatcga agagcaatca tttataaaag
    22981 caaagcggag aaagagacgt ctccaagggt tcggagtatc tttggctatc gtaatgatta
    23041 caacaagtag ccttcttttt aatagtcaag ttcgaagctt tgcagaagat ttacccatct
    23101 taggatcagt cattgaattg atcttaggcg aacgctttac tgatcgatct gaaaaaatcg
    23161 atattcaggt accgaagatc aatacacaaa atgagaaaga aaataaaaca attcatggat
    23221 taaatcaaaa atactttcgc gaaggacaag cagattttga aaaagctgaa aaagaatatg
    23281 gaaattttga aacggatcat taccaagttt tgggagatta tcagaaaatc gtagacgata
    23341 accggtttct cgtgattgaa aggcagatca cccaaacagc agcagatagc catgttgaaa
    23401 aaagatacga cacaatcgac aagaaaaaca gtgtccagct ctcacttcca cttttattca
    23461 aagatgacac ctatctatct gttttgacaa aagaagtcaa acggcagatg gccgaacaag
    23521 tcaaggaaga tccatctaag tactattgga ctgagcaaga tattcaagaa gggacgatag
    23581 aaaaaccaac acttgttacg cctacacgaa gtttttattt gaacaaagag catcaattag
    23641 ttttgacttt ttctcaatac gaaattgctc ctggttatat gggtacacca gaatttgtca
    23701 ttcctaagtc cgtgacaaaa acgattttgg catcggaaga ttatttggat cattagacga
    23761 gaagtagttg cgactagcag tttcaatttt caagtaagta caagtaaata ataagaagag
    23821 aaaaagagaa agaagagata tagtgaaaag aaattattta gttatcagta ttattgcttt
    23881 atccgtgctt ttgtttttcg aaattacttc ggtggcctcc ttgatcaccg aagtaaaagg
    23941 acaaacacac tcttcggagc attcgtcaca atctttcaaa caatcagaac aatcaaccat
    24001 taaaagccaa tcaactgaaa cagctaacgt caaagaagcc attcaagaat cagcggaaaa
    24061 ggtacaaagt cagtcatcac aatcaacaca agacagtaca aaaacgagcc gagatccggc
    24121 tgtcaaaagc gtcgcaatca gctttgatga tggaccagga gcgaccacaa caccgcagct
    24181 tctgcgaatc ttaaaagaaa aaaacgtgca tgccactttt ttcgtcttag gagaaaatac
    24241 ggcgcagcat cctgaaatcg tgaagcagac agctgaagca gggcacgaga ttggcaatca
    24301 cacatacgat catcagaatc tggccactct ttctgcccag tcgatgacgg aggaagtgac
    24361 gaaagcagac actgagatca aaaaagtcac tggcaaaacc cccacttttg ttcgtccgcc
    24421 ctatggttct gtcacgaatg taggagcaac gatcatccag cgaccgatca tcgaatggtc
    24481 tgttgattca gaagattgga agacaagaaa tcctgatttg attttgcaga agatccaagc
    24541 aacagtatac gatggggcaa ttattttatt ccatgatatc tatcctgaaa cgattcgtgc
    24601 cgttcctcaa gtgatcgatt atctgaaaga gcaagggtat cgcatcacga cagttgggga
    24661 tttattaggg catccaacgg ctgtcgagaa ctattatggc agaaacgatc atcggccagt
    24721 tcagtaagtg agacaagtat ctttaataaa taacacattt taataagtag ttgaacacat
    24781 aactacctta cttgcaaagg cttaacaata tctatacagg tacaacaaaa aactgtccac
    24841 caattttctt tggcggacag ttttcactta aaaataaaga agcaactatt cgtaaattat
    24901 gctaaaatag cagtagaacg cgtcctgagg gaatcgaacc cccgtctcaa gaaccggaat
    24961 cttacgtgat atccactaca ctaaggacgc aagcactggg ataattgtaa ctaaacagat
    25021 aaaaaattac aaggggattg gacaaaaaaa tgaaatttga gcaaaatttt tcacaaaaac
    25081 agcagcagac acagaaacta gccatgacgc aacagttgca acagtccatc caagttttac
    25141 aatataacag tgaggagctg ttggcattcg ttgagaatca agcaatggaa aatccattgg
    25201 tggaagttgt tgagccggaa tggcagccag attacataaa agcttcttca tcttcttatg
    25261 aaggagaaga gacaaattac ctaaatcaga ttcctgatac aaaagggtct ttatttgatt
    25321 cattgatcga acaagtccat ttgaattacc gagatacttt tttgaggaaa atcgttcttt
    25381 atcttgtaga atacatcgat ttgaacggat ttttgacgat tagtttagaa gaagccggaa
    25441 aagagattgg agctacgcct atccaaatgc tcgatgcgtt gactctgatt cagcaattag
    25501 aaccagctgg agtaggtgca cgtagtttgc aagaatgtct catgcttcaa acagaacgag
    25561 atgattatgc gccggaatta gcttatatcg tgttggaaga atgttttgag gaactggtgg
    25621 agcgaaaatg gaaagaaatc gcacaacgtt tttctgttga tctacatgct gttcagcaga
    25681 tattcgacta tttacaaacg ttgagtccaa gccctggccg gattttcgac cgtagcagcg
    25741 aattgtacat tattcctgat gtccgggtat tagtggacga cgataaaaat gtacaagtta
    25801 tctcaaaccg aaaaaatcag ccaaatatac gctttcaaga gagttacttc aaacaaatgt
    25861 cacaacaagc agataaagaa actgaaaatt atttaaaaga acggaaacag gaattcgaat
    25921 ggttgaagaa aacgatccta caacgtgggg atacaatttt gcgggttgct caagtcattg
    25981 tttcccggca aaaagctttc tttatcgaca aggaacgtcc aatcaagcct cttacgttaa
    26041 aggaagttgc aactgagata gatgtgcatg aatccacggt cagtcgtgca gtcaacggga
    26101 aatatttgga aacagacttc ggtgtatttg aattaaaaaa atttttcact acacgaattc
    26161 ctacgaatag tacagaacag acagaagacc tttcagcaga tacagcgaag aaaaagctgc
    26221 aggaattagt cgatcaagaa gacaaaaaca agccgttgtc tgaccagaaa ttggttgaat
    26281 tgctgaaaaa agatgagata gcgatctcaa gacgaacagt agcaaaatat cgtgatttat
    26341 taggaatccc gagttcatca aaaagaaaac gttatgataa ctaatcagtc aaaagactag
    26401 aaaaagtatt tcactttttc tagtcttttt agttgaaaaa cgagagcaaa actgttagac
    26461 ttacagtcgt gaggttgaga tttcccttta ttgatgtgaa attcgaatag ttaatttctt
    26521 tttttatcgt atatgggtcg ttttaagtct acattggacg taaagtgtcc aactaaggag
    26581 atcgctatgc tggatgaaat aaaaatgatt gaatctgttg caccagatat tatcgaagtg
    26641 ttgcaagaac gctataaaat tttacgaaat atctattgga tgcagccgat tggacgtcga
    26701 agtttatcgg aaagtatggg gatcacagaa cgagtattgc gaacagaaac agatcttttg
    26761 aaaaatctga atttgatcga cacctcaaaa agcggtatga cgctgacaac taagggcgaa
    26821 gaagtttatc aaagccttga aaacttcatg gatcagcttt taggtacgca tcaaaccgaa
    26881 caacagttgg cagaatattt tggtaaccaa cgctgtatcg ttgtttcagg aaacagcgaa
    26941 gagcagacca aagtggctga tgcattcggc gaagcgctga gcgaagccct agaccgacta
    27001 cttccagaag gcgagaatat catcgctgtg atgggaggaa ctactatggc tgtggtagcc
    27061 gaacaattgt ctaatctgga aaataagaaa cggcataacc tgtttgtccc tgcaagaggc
    27121 gggattggtg aagcgataac tgtacaggca aattctgtca gtgcaagaat ggctgctaaa
    27181 gcaaacggta accaccgtgc actttatgtt ccggaacaat taagtctggc aacttacaat
    27241 tctttactca atgagccatc tatccaagaa gtcttaaact tgattagtga agcgaattgt
    27301 gttattcata gtattgggcg tgcattacac atggcagcac gtcgtaagat gactgaaaaa
    27361 gaattagtca tgctgaaaca agcaaatgct gttgcggaat cttttggcta ctttttcaat
    27421 gaaaaaggtg aagtcgttta taaggtccct agaatcggca tagaattgca agatttggaa
    27481 aaagtcccga tcatcatggc aatcgcgggc ggaaaatcaa aagctaaagc aattcgagct
    27541 tatatgaaaa atgcaccaaa acaaacgtgg ctcatcactg atgaagctgc tgcaaatgag
    27601 attttaaaag gggtaaccct ttaaaataaa tatttttttg attttcataa ggaggaaatc
    27661 ttaaatgaca gttaaagtag gtattaatgg ttttggacgt attggacgtc tagcattccg
    27721 tcgtatccaa gatgtagatg gaatcgaagt agtagcaatc aacgacttga cagatgctaa
    27781 aatgttggca cacttgttaa aatacgacac aactcaagga cgtttcaacg gaacagttga
    27841 agttcatgaa ggttcattca acgtaaacgg aaaagaagtt aaagtattag ctaaccgcaa
    27901 cccagaagaa ttaccatggg gcgaactagg cgtagatatc gttctagaat gtactggttt
    27961 cttcacttct aaatcagcag ctgaaaaaca tttaacagct ggtgctaaac gcgttgttat
    28021 ctctgctcct ggcggaaacg atgttccaac aatcgtttat aacacaaacc acgaaacatt
    28081 aactggtaaa gaaacagtta tctcaggtgc ttcatgtact actaactgtt tagctcctat
    28141 ggctaaaaca ttgaacgaca aatttggtgt tgttgaagga ttaatgacaa ctatccacgc
    28201 ttacacaggt gaccaaatga ctctagacgg acctcatcct aaaggtgact tccgccgtgc
    28261 acgcgctgct gctgcaaaca tcgttcctaa ctcaacaggt gctgctaaag ctatcggttt
    28321 agtaattcct gaattgaacg gtaaattaga cggagctgct caacgtgttc ctgtaccaac
    28381 aggttcatta acagaattag taacagttct tgaaaaagaa gtaactgttg acgaaatcaa
    28441 tgcagcaatg aaagaagctt caaacgaatc ttacggatac aacacagacg aaatcgtttc
    28501 ttcagatatc gttggtatga cttacggttc attatttgat gctacacaaa ctaaagtaat
    28561 gacagttggc gacaaacaat tagttaaaac tgttgcttgg tacgacaacg aaatgtcata
    28621 cactgcacaa ttagtacgta ctttagaata cttcgctaac ttataagata gacaaacgta
    28681 caagtgtaaa ttatagacat gtacatttaa aagcggggaa gcaatcgcgc ttcttccgct
    28741 ttttttgata ataagtttat tcaggaggaa ataaaatggc taagaaaaca gtgaaagata
    28801 tcgatttaaa agacaaaaaa gttcttgtcc gtgttgactt caacgttcct ttgaaagacg
    28861 gcgtgatcac tgacgacact cgtatcaaag cagcattgcc aacaatcaac tacgttctag
    28921 aacaaggcgg aaaagcaatc cttttctctc accttggacg tgtaaaaaca gaagaagata
    28981 aagaaggaaa atctttagct ccagttgcaa aacgtctagg cgaattatta ggtaaagaag
    29041 taacattcgt acctgaaact cgcggagaac aattagaaga agcaatccgc aacatgaatg
    29101 acggcgacgt tgtcgtattc gaaaatacac gatttgaaga tattgacggt aaaaaagaaa
    29161 gcaaaaatga tacagaacta ggtaaatact gggcttcatt aggcgatgta tttgttaacg
    29221 atgctttcgg tacagctcac cgtgcacatg cttctaacgt aggtatcgct tcaactggta
    29281 tcccaacagt tgctggattc ttgatggaaa aagaaatcaa gttcatcggt gaagcagtag
    29341 aagaaccaaa acgaccaatg attgcaatcc ttggtggcgc aaaagtttct gataaaatcg
    29401 gcgtaatcga aaacttgatt cctaaagcag ataaaatctt gatcggtggc ggaatgactt
    29461 acacattcta cgaagcaaaa ggaatcaaga tcggtaactc tctagtagaa gctgataaag
    29521 tggaactagc aaaagaatta atcgaaaaag caggcgacaa acttgtcttg ccaatcgaca
    29581 atgtctgtgc accagaattt tcaaatgatg ttgaaacaca agtaatcgaa ggagacattc
    29641 ctgatggttt gatggcttta gacatcggcc cagcatcagt aaaattattt gctgatacat
    29701 tacaaggtgc aaaaacagtt gtttggaacg gacctatggg tgtatttgaa atgagtaact
    29761 ttgctaaagg tacaatcggt gtttgtgaag caatcgctaa cttagaaggc gcaacaacaa
    29821 tcatcggtgg cggagactca gctgcagcag cagaacaatt aggatttgct gacaaattca
    29881 cacacatctc aacaggtggt ggcgcaagct tggaattgct tgaaggtaaa gaattaccag
    29941 gactagcagc aatcaacgat aaataaacga ccaagtttgc tcaattctga cgagtaatcg
    30001 atcaaaaggt gaagggagtt ttcatgactc cctagccatt tttcaataaa ttaaggagtg
    30061 tgttcctcaa tgcgtaaacc tattatcgca ggtaactgga aaatgaacaa aactgcatca
    30121 gaagcaaaag aatttgctga agcagtaaaa aacaaaatcc cttcaaatga caaagttgat
    30181 tcagtcatcg gatctcctgc attattctta caagaattaa tgacatcagc tgaaggtaca
    30241 gaactaaaaa tctcagcaca aaactgctac tgggaaaact caggtgcgtt cactggtgaa
    30301 acttcaccag cagctcttgc tgatctaggt gttcattatg tgatcatcgg acattcagaa
    30361 cgtcgtgaat atttccatga aacagacgaa gacatcaata aaaaagcaaa agctatcttt
    30421 gcaaatggta tgacaccaat cctatgctgc ggcgaatctt tagaaaccta tgaagcaggc
    30481 aaaactgctg aatggatcga aggacaaatt acagctggtt tagctgatct ttcagctgaa
    30541 caagtgtcaa acatggttat tgcttatgaa ccaatctggg caatcggtac tggtaaatca
    30601 gctgacgcac aaatcgctga cgaaatttgt ggagttgtac gtcaaacagt tgaaaaactt
    30661 tatggaaaag aagtatcaga agcagttcgt atccaatacg gcggttctgt aaaaccagaa
    30721 aatattgctg aatacatggc aaaagaaaat gttgacggtg ctttagtcgg tggcgcaagc
    30781 ttgcaagcag attcattttt gtcattgtta gacgctgtaa aataattgtt gaaaatttgc
    30841 gaaaaatgca agaaatgttt gcactaatga gcaaaattaa ctaaaatcgt atatgaggga
    30901 aatcccttag gaaaaaacaa actcaaagga gagacaaaac atgtcaatta ttactgatgt
    30961 ttacgctcgc gagatcttag actcacgtgg taacccaaca atcgaagtag aagtatacac
    31021 agaaagcgga gcttttggcc gcggaatggt tccttcaggt gcttcaactg gtgaatacga
    31081 agcagttgaa ttacgtgacg gagacaaagc tcgttatggc ggtaaaggtg taactaaagc
    31141 tgtcgacaac gtaaacaaca tcattgctga agcaatcatt ggctacgatg ttcgtgacca
    31201 aatggctatc gacaaagcta tgatcgcttt agacggaact cctaacaaag gtaaattagg
    31261 tgcgaacgct attttaggtg tttctatcgc tgttgcacgt gcagctgctg actacctaga
    31321 agtaccttta taccactacc taggcggatt caacacaaaa gtattgccaa caccaatgat
    31381 gaacatcatc aacggcggat cacatgctga taactcaatc gacttccaag aattcatgat
    31441 catgcctgtt ggcgctccaa cattcaaaga agctctacgt atgggtgctg aagtattcca
    31501 cgcattagct tcaatcttga aagctcgcgg attagcaact tctgttggtg acgaaggtgg
    31561 atttgctcca aaccttggtt caaacgaaga aggttttgaa gtaatcatcg aagcaatcga
    31621 aaaagctggc tatgtacctg gtaaagacgt tgttcttgct atggatgctg cttcttcaga
    31681 attctacgac aaagaaaaag gtgtttacgt actagctgac tcaggcgaag gcgaaaaaac
    31741 aactgacgaa atgatcaaat tctacgaaga attagtttct aaatatccaa tcatctcaat
    31801 cgaagatgga ttagacgaaa acgactggga cggattcaag aaattaactg acgtattagg
    31861 cgacaaagtt caattagttg gtgacgactt gttcgtaaca aacactcaaa aattatctga
    31921 aggtatcgaa aaaggaattg ctaactcaat ccttatcaaa gtgaaccaaa tcggtacatt
    31981 aacagaaact ttcgaagcta tcgaaatggc taaagaagct ggctacacag cagttgtatc
    32041 tcaccgttct ggtgaaacag aagattcaac aatctctgat atcgctgttg caacaaatgc
    32101 tggccaaatc aaaactggtt ctctatcacg tactgaccgt attgctaaat acaaccaatt
    32161 actacgtatc gaagaccaac ttggtgaagt tgcagaatac aaaggtttga aatctttcta
    32221 caacttaaaa aacaaataat tcttttctaa tgaagataca tgatcgacta ttgaaagata
    32281 gtcgagtatg atcacttagt taagtaatcg aactctctac ttgctgaaat atcagcaggt
    32341 agagagt
//
LOCUS       DAZKMB010000016        32268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000016 DAZKMB010000000
VERSION     DAZKMB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.016"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..801
                     /gene="truA"
                     /locus_tag="ACG8V3_000720"
     CDS             55..801
                     /gene="truA"
                     /locus_tag="ACG8V3_000720"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0299283.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(1256..3925)
                     /locus_tag="ACG8V3_000721"
     CDS             complement(1256..3925)
                     /locus_tag="ACG8V3_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0299284.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(4163..4351)
                     /gene="rpmB"
                     /locus_tag="ACG8V3_000722"
     CDS             complement(4163..4351)
                     /gene="rpmB"
                     /locus_tag="ACG8V3_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0299285.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            4621..4983
                     /locus_tag="ACG8V3_000723"
     CDS             4621..4983
                     /locus_tag="ACG8V3_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0299286.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5035..6717
                     /locus_tag="ACG8V3_000724"
     CDS             5035..6717
                     /locus_tag="ACG8V3_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0299287.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            6834..8870
                     /gene="recG"
                     /locus_tag="ACG8V3_000725"
     CDS             6834..8870
                     /gene="recG"
                     /locus_tag="ACG8V3_000725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0299288.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            8919..9920
                     /gene="plsX"
                     /locus_tag="ACG8V3_000726"
     CDS             8919..9920
                     /gene="plsX"
                     /locus_tag="ACG8V3_000726"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0299289.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            10042..10284
                     /gene="acpP"
                     /locus_tag="ACG8V3_000727"
     CDS             10042..10284
                     /gene="acpP"
                     /locus_tag="ACG8V3_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0299290.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            10552..11556
                     /locus_tag="ACG8V3_000728"
     CDS             10552..11556
                     /locus_tag="ACG8V3_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299291.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            11557..12501
                     /locus_tag="ACG8V3_000729"
     CDS             11557..12501
                     /locus_tag="ACG8V3_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299292.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            12501..13463
                     /gene="opp4B"
                     /locus_tag="ACG8V3_000730"
     CDS             12501..13463
                     /gene="opp4B"
                     /locus_tag="ACG8V3_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0299293.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            13490..14404
                     /locus_tag="ACG8V3_000731"
     CDS             13490..14404
                     /locus_tag="ACG8V3_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0299294.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            14430..16211
                     /locus_tag="ACG8V3_000732"
     CDS             14430..16211
                     /locus_tag="ACG8V3_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0299295.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            16559..17245
                     /gene="rnc"
                     /locus_tag="ACG8V3_000733"
     CDS             16559..17245
                     /gene="rnc"
                     /locus_tag="ACG8V3_000733"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0299296.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            17265..20846
                     /gene="smc"
                     /locus_tag="ACG8V3_000734"
     CDS             17265..20846
                     /gene="smc"
                     /locus_tag="ACG8V3_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0299297.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            20855..21664
                     /locus_tag="ACG8V3_000735"
     CDS             20855..21664
                     /locus_tag="ACG8V3_000735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0299298.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            21675..22673
                     /gene="ftsY"
                     /locus_tag="ACG8V3_000736"
     CDS             21675..22673
                     /gene="ftsY"
                     /locus_tag="ACG8V3_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0299299.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            22860..25127
                     /gene="gshAB"
                     /locus_tag="ACG8V3_000737"
     CDS             22860..25127
                     /gene="gshAB"
                     /locus_tag="ACG8V3_000737"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0299300.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(25216..25668)
                     /locus_tag="ACG8V3_000738"
     CDS             complement(25216..25668)
                     /locus_tag="ACG8V3_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0299301.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            25818..26768
                     /locus_tag="ACG8V3_000739"
     CDS             25818..26768
                     /locus_tag="ACG8V3_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0299302.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            26761..27720
                     /locus_tag="ACG8V3_000740"
     CDS             26761..27720
                     /locus_tag="ACG8V3_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0299303.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            27717..28472
                     /locus_tag="ACG8V3_000741"
     CDS             27717..28472
                     /locus_tag="ACG8V3_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299304.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            28495..29448
                     /locus_tag="ACG8V3_000742"
     CDS             28495..29448
                     /locus_tag="ACG8V3_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0299305.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(30092..30388)
                     /locus_tag="ACG8V3_000743"
     CDS             complement(30092..30388)
                     /locus_tag="ACG8V3_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0299306.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            30743..31099
                     /locus_tag="ACG8V3_000744"
     CDS             30743..31099
                     /locus_tag="ACG8V3_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0299307.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            31109..32008
                     /locus_tag="ACG8V3_000745"
     CDS             31109..32008
                     /locus_tag="ACG8V3_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0299308.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        10682 a         5727 c         6896 g         8963 t
ORIGIN      
        1 ttatagaaaa atcattattc cagataacct tttaggaaca aaggagagaa aaacatggta
       61 cgttacaaag caatcattgc gtacgacgga acaaatttta atggattcca aaaacagcca
      121 aatgggcgta ctgttcaaga agaagtagaa aaaacattac aaaaaatggc aaatggaaaa
      181 gaaatcacgg tctttggttc gggaaggaca gatgccggag tacatgcgat cgggcaagta
      241 atccattttg attatccaga ggagcgtccg ttagaacgaa tgcgtttcgc gctagatacc
      301 cagtcaccag aggatattgc tgtaagacaa gtagaaattg tttcagaaga cttccacgca
      361 agatatttgg tcaaagaaaa gacgtatcaa tttcgagtgg atatagggaa accacgcagt
      421 ccttttcgac gccattatgc aagttatttt ccttacccac ttgatttgga aaaaattcag
      481 cgggcgcttc ctgatttatt agggactcac gatttcacct ctttttgtgc atcaggaagc
      541 tcgatagagg ataaaatacg tacgatctac gaggctaaga tggaggtaaa tgaaacaaaa
      601 gacgaattgt tgtttacatt taggggaaat ggcttcttgt ataagatgat acggatctta
      661 gtaggaacgc ttttgaaaat tggcaatggc cgattgcctg aagatagtat cccagatatt
      721 attgcgaaaa aagatcgtaa tgcagcagga cctacagcac accctgaagg actttatttg
      781 tatgaagtga aatatgaata agaggttatg atagaagtgg tcagcttcaa gaaataagaa
      841 ggtatttacg aaaattactt ctcaaaattt tgcaaaattt cggcttattt tcgaagaagc
      901 tacttctgga acaccgttta aggaagaggt cgtgaaatca gcgttttgac ctgagtatta
      961 gagaaacaaa cgggaaaata gctcctcata ttttgccgtt tgttgagcta ataccgaagg
     1021 tcagctgatt gaacaccgtt taagaaagag gtcgtgagcc tgtcatttaa ctctgagtca
     1081 caaagagcaa tttcaaaaaa cagcttctca tctttgatga atatcgtgac ttgtggacga
     1141 ggcgttgata agtgaatcat ctttttgtta cctatttttt ctgtaaccaa aaaacgatgg
     1201 aacgttgttt tgattgccct tggtgcggac agtctgaaca ggttccatcg tttctttatt
     1261 ttctacgtct tcttctgtgt gtgtttcgtg gttgttcttc tattgctgga tcaatcgtgc
     1321 cataaaaggg gcgattgcgg tcaaggtacc agacataaag aataaacaat gcaatacata
     1381 ggacaaaaag gattgcacct atcgtaaatc cttcaggcaa gtaactgaaa gtgacagtat
     1441 gtttacctgc tggaactttt atcatcaaga atgcgtcttt gaaattgctg accttgactg
     1501 gtttcccatc aattttagct ttccatcctt tgtcataagg aatagttgtc accaacatgg
     1561 aatcttcagc ggtatttaca gtcccagaag cttttctgcc agtggcttcc atttcgacac
     1621 ctttttcttt gatctgattc attgcggctt gataggctac aacatctaga ccaacgactt
     1681 taggctccat gaaacttatt tctttcgtcc cataaaaact tgcagtaaat gtcactgttg
     1741 tctcttgagg ataatagcca agatcatagt attgaccaga aatgttgatt tgcgtttttc
     1801 gcgatgtacc attgacagtg atcgtcgcac tagaactttc taattgcgca aaattggttg
     1861 ggaacaaact taagtaagct tgtgtatttg caggcacatg gacttcccaa gtgagctctt
     1921 ttggctggtt atctgctgtt tctgagtaag tgacgccagt cgttgtcttt gtgatcgtcg
     1981 tattcaactg attcttcaaa gtaggtgtat agaatgtaaa atactgttca tttagaccag
     2041 acaatgtatt gatcaagttt gtctgactgg tcaaattatc tgtcactggc tgctcaacat
     2101 cattaacgga caacggtgca gtaaatgcta gtggcaatgc attgacattc tgatacagtg
     2161 tatagtcgcc gctttgacca gcttggcgga agccatattt actgaacgtg ctttcgtttt
     2221 tagaaatgtt atatttgatt cctgtaaacc catccatcag caaagtatta ttgttataac
     2281 ggatattcaa atttgtccct cttgaacgga aacctaattt atccaggtaa gaggaagaat
     2341 tacgatttct tatagaagaa aacaaactga taccactgta accatagttg atcgaatcgt
     2401 ttgcagatac tggatccaga ttttccaagc ggtaaaacga gtcggaatct ttttttgttt
     2461 catttacaag agctttcaag tcaggataag gatcagtata taaactgcgg gaagcataat
     2521 tccaatcatc aagaatgcca tggatcatac tgttcgtatt gaccagtgct tcagcagaca
     2581 tgatcaataa taacaatata ctcagatgat tgaatgaaag ttttttatgc tgataaaatc
     2641 ccacagcaaa agcataaatc cctaagaaaa ccactgtcaa tacaaaggaa gtcatcgtca
     2701 cataggtgta gcttccttca ggtttgatgc cccatgccac cgcaaagaga gcagctacga
     2761 ccaaaatgat tcctaacaac agcccacgat cgtcttgttc tatcttttcc catccttttc
     2821 ctgcaagcag gagcactaag aaagagaaca gataggcata acggaataaa aacatattcg
     2881 gtgcatgcat accatgccag aacaaattca atggaacaag ataaaaactt gcgataagta
     2941 tggcgaaaag actgccgtac aaaaatttat ttttcaacgg tactttccga ctggcaaaat
     3001 aaaacagaca gaggactaat ggtagcagac ctgcgtaaac gaacggaatc gatccgtatt
     3061 ttgtcgtgtc ataaacacca atcatatttt tcatgattag atccagataa gaagtcgctt
     3121 ctgttttcaa cgtagtgatc tgactcagtg cctcgccatt tgaccgaaga tccagcactg
     3181 ctggcaatac aatgatcatt gaagcgccgc ctgccagtaa agaagtgata ccataaggaa
     3241 caatcgtttt tttatattct gaccagtttg tcaatagacg ggcgataaaa tataagaaag
     3301 aaaatacgcc aatcataaaa cccatataaa aactggataa aaacaagagt aaataactga
     3361 taaataaaag tttcggccgt ttaagatcca taacacgatg aatccccaaa atcactaatg
     3421 gaagataaaa aaatgcatcc agccacatga tgatctccga atgtgcagtg ataaatgaga
     3481 tcaatgcgta actgacgcta agtgcaacat gcccccagcg aggaatcttg aacgtacgtg
     3541 caagaaacca aaaagccaat ccggcacagc cgaccttaag caaagtcaga acatacagtg
     3601 catctggcat catctggttt ggaaaaaaga acaccagtgg cgtgaagatc ccgcctagat
     3661 agtaagaaat taaagataaa taattcaatc ctagagaagc attccatgta taaaaaatac
     3721 tttgctttcc aagaagaaca tttcggaagc tggcatgaaa attagaaaac tgtgaaaagg
     3781 catcacttgc caaaatactt ctgctgcttc caggatagat tccgatactt agataaacaa
     3841 gtgctaaaat caaaaacgga atcaggaaac tagctgctgc gtagcgtccg ttttctctaa
     3901 agaatgactt taaatttttt ttcatattta ttttccacca aatttctatg aagttgtatt
     3961 cagtttatca tatccttgtt tttctctgct taaaaagagt ctagttttca caaaaaaata
     4021 aaatttagaa taaataactt cataatttgt tcgtataaca aaaataaacg caaaaaaagg
     4081 gtaggaatca aaagtcccac ccttaaaaat ttttattaaa taaaaaaccg ttcactatct
     4141 gaacggtcca agtatcattc aattaaacgc gttcaacttt tcctgatttc aaagcacgag
     4201 ttgacaccca aactttttta ggttttccgt cgaccataac gcgaactttt tgtaagttag
     4261 gtttaacagt acgttttgtt gagttcatag cgtgtgaacg gttgtttcca ctttttgtct
     4321 tgcgacctgt aaagtaacaa acttttgcca tggtaatttc ctcctttgcc ttgatttgga
     4381 gcaatagaat aattcagctc ataccagact aacatatcat atagaatggg aatttgcaag
     4441 tacattttca tagatacaat tgacaattga gaagaataat tgtaaattaa acaaggttat
     4501 acctgaaatt ttgcaaggtt tttgagtact ttgggtaact gccttttatt tcaagttgcg
     4561 ctatgataaa atatgataga agaaaaatgg gagtttttta cccgttaagg aggctcattc
     4621 atggctgtaa aaattcaaac gtcagctgga accattgaaa ttaccaatga tgtaattgca
     4681 acagttgttg gtggcgccgc gacagatgtg ttcggtgttg taggaatggc cagcaaaaat
     4741 caaattaaag ataatattac tgagattttg cgtaaagaaa attactcaag aggagtagtt
     4801 gtccgccaag aagataatgg tattgctgta gatgtttata cgattatcag ctatggtacg
     4861 aaaatctctg aagtttcccg caacgtccaa gaaaaagtga aatacaatct agaggcatta
     4921 ttaggcgtaa ctgccaattc agttaatgtc tttgtacaag gtgttcgtgt actgccagac
     4981 taggcaggag actggctggt aactcagcag tcgatctgaa ggaggattat ctaggtgagt
     5041 gtaacagaaa taagtgcaag ccagttccag gaaatggtcc aggcaggtgc gaatcgtctg
     5101 caagtaaatg cagaatacgt caattcatta aatgtattcc ctgtgccaga tggcgatacg
     5161 ggaacaaata tgaatctatc tatgactagt ggagctaagg cagtagtaga ttcaacctca
     5221 gaaaaagtag gagaattagc agcaatgctt tcaaaaggat tgctgatggg tgcgcgcgga
     5281 aattccggcg ttattttgtc tcaattgttt cgtggatttt ctaagcaaat tccagatgtt
     5341 gtctcattaa atgcttctga tcttgctgca gccttcactc atggagttga aactgcgtat
     5401 aaagcagtaa tgaaaccagt agaaggaacg attttgacag tagctagaga agcagcgaaa
     5461 gctggagaaa agaaagcaaa aagtactgat gatgtcgttg aagtaatgac agctgttgta
     5521 aaaggagcaa agcgtgcttt agctaaaact ccagacttgc ttccagtcct aaaagaagtc
     5581 ggtgtcgtag acagtggagg gcaaggactg ctgtttgttt atgagggctt tttaagtgca
     5641 ttgaatggag aattccaagc ggatgaaaca tatgaacctt ctccagcaga aatggatgat
     5701 atggtcaatg cagaacatca tcgcagtgtc caaggtcaaa tggcgacaga agatatccat
     5761 tttggttact gtacagaaat catggtaaaa atcggagaag gacctacagt cgatagtaca
     5821 ttcgattacg aagaattccg taactattta gatggtatag gagattcttt acttgtggtc
     5881 aatgatgacg agatcatcaa agtccacgtg catacagaaa acccaggaga agtcttgaat
     5941 tatggacaaa aattcggttc attagtcaaa atcaaagtcg acaacatgcg cttgcagcat
     6001 gagacgattt tggaacacga tgaagaagtg aacgagtttg cgaaaaaaga aataccacgt
     6061 atcccgtatg gtatcgtagc gatcgctgct ggtaaaggcg tccaagagct gtttgaaagt
     6121 ttaggcgcta attatgttat cagtggtgga caaacaatga atccaagtac ggaagacatt
     6181 gttaaagcga ttcaagaagt aaacgcagat aaagtgattg tcttgccaaa caataagaac
     6241 atcttcatgg cagctgatca agcagcagaa gtagcagata tcccagtagc agttgttcct
     6301 tcaagaacga tttcccaagg attgacagct atgctttcat tcaatgatca agcttcttta
     6361 gaagaaaaca aagaaacgat gacagaaatg ttgtctagcg ttgtgagcgg tcaagtcact
     6421 cacgcgatca gagatacctc aattgacggt gtagagatca aagaaggaga ctttttgggc
     6481 atgatcgatg gaaaaatcct gatctctaac ccagatatca cagcaacagc gttaagtaca
     6541 ctagaacaaa tgatcaatga ggacacagag atcgtaacga tcttggcagg tgatgaagga
     6601 acagttgaac aagcaaatga atttgctgag aaactaacag aaaaacatcc tgatttggaa
     6661 atcgagatcc accaaggaga tcagcctgtc tatccttatc tattttctgc tgaataattt
     6721 ctgtgtcaga atatctttct aacacagtat tcattcataa aaggccttcg gttagtgatc
     6781 aggtatgatc gctgaccaag tccttttttt gttaaaggag ggaggaaaaa tttatgcgtt
     6841 ctttagatga tccaatcact atgctggcag gagtcggaga aaaaagagca gctagtcttg
     6901 ccagcttggg catccaaacg attgaagact tactgactta ttatcctttt cgctacgaag
     6961 atattcagga aagaaatcta aacgagatcc aggatcaaga aaaggttact ttgaaaggaa
     7021 tcgtagtctc gcctcctgtg atgaatcgat ttggttataa aaaaagccga ttgcaatttc
     7081 gtatgatgca agaacatgat gtgttcaacg tttctttttt taatcaacct tatctaaaag
     7141 aaaaagtcat tttatcggag gaaatcgccg tatacggtaa atgggatgca aagcgtaaag
     7201 ccttgaacgg gatgaagatt ttaggttctc aaacaatgga tgacttttcg ccgatatatc
     7261 acgtcaacaa atcaattcgt caaacgacat tagttgatct aatcaaaaaa gggtttcaag
     7321 aatacggcac attgattgaa gaaaatctgc caaacaacct tgtggagaaa tatcgactgc
     7381 ttgaccgagc ttctgctgta agaagtatgc attttccaaa agaccacgaa gaaaaccatc
     7441 aagccaaacg acgtgtcgtt tttgaagagt tctttttatt tcaaatgcgt cttcaaggat
     7501 tgaaaaaagc agaaaaagca gaaacgaatg gtttggagat cctttatgat gtccaaaaat
     7561 tgaaacaatt tactcaaaag ctaccttttg aactgacaaa tgcacaaaaa cgtgtaacga
     7621 atgaaatctg tcgggatctt cgcagtccgc agcatatgca gcgacttttg cagggagatg
     7681 taggtagcgg aaaaacagtg gttgccgcga ttgcgttata cgctaccgtt acagcaggtt
     7741 tccaaggtgc gttgatggtg cctacagaaa tcttggcaca acaacatatg gaaagcttgc
     7801 aacagctgtt tgatcccaat gaagtaacca tcgcattgat tacggggtca acgaaagcaa
     7861 aagaacgacg tgagctcctt gaaaaactag agcaagggga aattaacgtc gtgatcggca
     7921 cacatgcatt gatccaagat ggagtcgaat ttcagaattt aggtctagtt attacggatg
     7981 agcagcaccg ttttggcgtg aaccagcgga aagtacttcg tgaaaaaggc tggcgaccag
     8041 atgtcttatt tatgacagcg actccgattc ctagaacatt agctattaca gcatatggtg
     8101 agatggatgt atctatcatt gatgaacttc cagctggacg tatcccgatc gaaacacgct
     8161 gggtaagaac acctcaattg gacagtgtac tggattggac gaaaaaagaa ctggcaagag
     8221 gacatcaaat gtatgtcatc tgtccgctga ttgaagaatc agaagcatta gatgtaaaaa
     8281 atgcagtgga aatctatgaa aaacttcgtg aactatttgc accaaaatat gaagttggct
     8341 tgttacatgg taaaatgaag aatcaggaaa aagaagtaat catggaagcc ttcaaagaca
     8401 accagatgca gatattagtt tcgactactg taattgaagt aggggtaaac gtgccgaacg
     8461 cgaccgtcat gctgatcatg gatgcggatc gatttggatt ggcacagctg caccaacttc
     8521 gtggacgtgt tggacgcgga agtgatgctt cctactgtat cttagttgct aatcctaaaa
     8581 atgaactagg tgtggaacgg atgaaaatca tgacagagac caccaatggt tttgtactaa
     8641 gcgaaaaaga tctggaatta cgtggtcctg gtgaagtatt tggcttccgt caatctggtc
     8701 tgcctcaatt tgcgattgct gacttggtga cggatggaaa tgttttggag gtagccagag
     8761 aagaggctgc agccctttgg caaataaagg attggcagat gcttcctgag tatcagcctt
     8821 tagcgcaaag cctccaaata gaaaatcagg ataatcgtta tttcgattag cagactgtgc
     8881 tatactttat actgatgacg gaaaaaggag tggaaacaat gaaaattgcc gtagatgcaa
     8941 tgggcggaga tcacgcacct caagcaattg tagaaggcgt ggctttagcg cagcaagatt
     9001 ttccagaagt agaattctta ctttacggaa aagaagcaga aatcaagaaa tacttaacaa
     9061 atgaaaaaaa catcacgatt atccatacag acgaaaaaat caatagtgat gatgagccag
     9121 taaaagcaat tcgacgaaaa aagactgcat caatggtcct tgctgcacaa gcagtgaaaa
     9181 acggtgaagc agatgctgta ttttcagcag ggaatacagg cgcgctacta gcagcagggt
     9241 tgttcatcgt aggacgtatc aaaaatatcg aacgccctgg cttgatgtct accttgccag
     9301 tagtagggaa agaaggtgcc ggtttcgata tgctggatct tggcgctaac gcagaaaaca
     9361 aaccagaaca tttgttgcaa tacggtattt taggttcttt ttatgcacgt aaagtccgag
     9421 gtatcggacg tcctcgtgtc gcactcttaa acaacggaac agaagcaaca aaaggtagtg
     9481 aagtgacgaa aaaagcctat gaactccttc agaatgaaac aagcttgaat tttattggaa
     9541 acgtagaagc tcgtgagcta ctcaatggtg tggctgatgt agtggtcaca gatggattta
     9601 caggcaacgc tgttttgaaa tctattgaag gaacagcaat gaatatgatg aatctattaa
     9661 aatctgctat tctaaatgaa gggatcaaag gaaaaatggg cgctatgctt ttaaaagacg
     9721 gcttacgttc tttgaaagca gaaatggatt attccaaaca tggcggtgcc gttttgtttg
     9781 gcttgaaagc accagtcatt aaaactcatg gagcaacagg ccccgatgct gttcgctata
     9841 cgatccgcca aatccacaca atgcttgaga cagatgtagt tggacaatta gtcgaacaat
     9901 ttgaaaaaag cgaagaatag tatttggata tctgaagcga tccgttccat tgaaaaaaag
     9961 gtggacattc ctgaaaaatg ccagtaaaat tagccatggt gtaggtaatt gacaagtgta
    10021 tgattcttgg aggtgaaaaa gttgactcgc gaagaagtat ttaacaaagt agcgaaaatt
    10081 atttcaaatc attttgagat agataccgat aaagtgacgg atgaattgaa catcaaagat
    10141 gatttaaaag ctgattcaat tagtattatg gaattcgttt tagagcttga agatgaattc
    10201 ggtacggaaa tttcagatga agacgctgaa caaattgaaa cagtcggtgg cgcagtagat
    10261 tatatcagca gccatcttaa ataatttcaa ataaaagcaa agaaggataa aaagctgcat
    10321 gcagcttttt atccttcttt ttttaatctt tttgacacgt aaaagtcatt tttctgataa
    10381 ttctggcgat aaacagaaat tatgttgcaa agtaaagcgg ttttctatat aattattaat
    10441 aattcattac acaaagataa gaataacctt tgttataaaa cgatttattc tgtgtaatgt
    10501 ccttttttat taagaaaaac ttccatttag gaagcagaga ggggtattcg attgtccgat
    10561 aaccaattat tagaagttca gaacctacat acaggattcc gactcaaaga tgactattac
    10621 gatgcagtag atgacgtttc atttacttta gacaaaaacg agattttagc aatcgttggg
    10681 gaatctgggt gcgggaaaag taccttggct actacgatta tgggattact tgatcctaat
    10741 aacacaaaaa ttacaggtga gattttatat aacgatctga atttaatcga cctgaacgaa
    10801 acgctctata ataaaatccg cggaaatgat atcggaatga ttttccaaga tccgttgtct
    10861 gcattaaatc ctctcatgag aatcgaagac cagatcaaag aaggactttc ctatcacaca
    10921 aagatgaatg cagaacagcg tcaagctcgt gcactagaat tgctagaaca agtagggatt
    10981 cctaatcctg cgagagttgg acgccaatat ccccatgaac tatcaggcgg tatgagacaa
    11041 cgtgtcatca tcgcaatcgc aatcgcctgc aaacctccaa tcgtcattgc agatgaaccg
    11101 acgacagccc tagatgtaac catccaagca cagattcttg atttactgaa agaccttcag
    11161 gaagaaacaa aaacagggat catcttgatc actcatgact taggagtcgt agcagaaatg
    11221 gctgatcgag tggcagtgat gtatggcgga cagttcgttg aagtagcagg tgtaaaagaa
    11281 ctgtttgatc atcctaagca tccttataca cgctctttac tgaactctat accacaagaa
    11341 ggaaatcatg atgcagaact ccatgtgatc gaaggtgtcg ttccttctct gaaaaacatg
    11401 cctcgtaaag gttgtcggtt tgcaccacgt attccatgga taccagcaag cgatcatgaa
    11461 gaagagccta tccttcatga agtcgcaccg aaccacttgg tacgttgctc ctgctataag
    11521 catttccact ttagagacga aaaaggagag gtgtagatga cagaattaat cacaatcaaa
    11581 gacctgaaag ttcattaccc tattcgcagt ggattcttca atcgagtaac ggatcatgta
    11641 tatgctgtag atggtgtgga ttttattatc gaaaaaggaa agacatatgg tttagtggga
    11701 gagtctggct ctggaaaatc tactacggga aaagccgttg tgggattaga aaaggtaaca
    11761 gccggacaaa tcatgtatga aggaaaagat gtaacgaagc gaagcaaccg caaaaaaatg
    11821 ggttataaca aggatgtgca gatgatcttc caagattcaa tgtccagttt gaatcctaaa
    11881 aagcgagtgt tggatatcat tgctgaaccc atccgaaatt ttgaacggtt aagcgatcaa
    11941 gaagaaaaga aaaaggtaaa aggactgcta gatatcgttg ggatgccgga agatgcgcta
    12001 tataaatatc ctcatgaatt ttcaggcgga caacgtcaac gtctaggagt agcccgtgct
    12061 gttgctacca atccaaaact gatcgtagcc gatgaacctg tatctgctct ggatctatct
    12121 gttcaagcgc aagtattgaa tttcatgaaa cgaatccagc aagaatacgg cctcagctat
    12181 ttattcattt cacatgattt aggtgtagta aaacacatgt gcgacaatat cgcaattatg
    12241 tacaaaggtc gatttgtaga aatcggtaca agagaagaca tttataatga cccgcgacat
    12301 atctatacga aacgtctgct ttcagctatt ccgcaaatcg atgtagaaaa tagggaaagc
    12361 cacaaagaaa atcgacgaag agtagaacaa gagtatatcc aaaaccaaaa agaatactat
    12421 gatgcgactg gtcgagtcta cgatttgcgt acgatcacaa ctacacacaa agtggcgttg
    12481 aaagatggag gtgcaagcta atgtggaaaa cgattctgcg acgtgtttta ttgatgatcc
    12541 cgcaagttat tattttaagt gttctgatct tcttattagc ccaagcgatg ccgggggatc
    12601 catttaccgg tttgatcaat ccaaatcagg atccggcagt gatcgaacag atgcgacaag
    12661 cagctggctt gaatgaccca tggtatgtcc agtacttccg ttggatcgga aatgctttgc
    12721 aaggcgattt tggtcaaagt ttcttataca aacgctctgt tgctgacttg atcggcgaac
    12781 ggatcatcaa cactttatat ctatcgatct tgacagttgt ccttacttat ttgattgctg
    12841 tgcctctggg gatgcttgct ggccggtatc aaaattcaat cttggataag attgtcgtaa
    12901 tctacaactt ctttagtttt gctgttcctt tatttatctt cggcttgatc atgctatttg
    12961 tttttggtta tcgtttaggc tggttcccaa caagtggatc acaaacacta ggtttgcaag
    13021 gtggatttat cactcaatgg atagaccgtt tgaaatatat tctgttacca tcaattaccc
    13081 aagcattttt ggcgactgcc gtcacaatcc aatatctgcg aagtgaagta atcgattcga
    13141 aatcgctcga ttttgtcaga acagcccgtt ccaaaggggt accgacgaat aaagtattca
    13201 gtcgacatat tttccgaaat gcagcgttgc cgatggcttc tcaaatgggg tatgaaatca
    13261 cttcgttgat tgctggttct gtagttatcg aaaagatttt tggttatccg ggagtcggaa
    13321 aactatttat tgattccatc ggccaacggg actataccgt gatcacatcc ttagtattga
    13381 ttttaggaat cgcgacgcta gtggggacat tactttcaga tatcatcatg agtatcgttg
    13441 acccgcgtat tcggattcaa taaaaccaat cagaaaggag aatggatcga tgagtgaaaa
    13501 aaacaataca gcagttcaag aaagtattcc tcctatgggg atacgaatga ttgctcgaga
    13561 atttaaaaaa gataaaattg ctattttttc attaggtttg ctagtggttt tattattagt
    13621 gatctttatc ggtgcgatgc tgaccgatca agacaaagtg atgtatgtta gtatcttcga
    13681 caaatacgct gcaccaggaa cagtttcgac acaaggacaa aagttcattc tgggagcaga
    13741 tgaaggcgga cgtgatgtat taggacaatt gatcataggt gctagaaact ctgttacgat
    13801 cggatttgcg atcacgatcc ttacttcgat catcggggtt ggattaggga tcatctctgg
    13861 ctactacgga gggatcattg acaacatctt gatgcggatc gttgatttta tcatgattct
    13921 accgatcatg ctgatcatta tcgtcttcgt atcgattatt tccagataca atgtctggtc
    13981 ctttatattc atcatgagtg cattttattg ggtagctaaa gcccgattat tccgaagcaa
    14041 gacattatct gaagcaagaa gagattatgt cagtgcctca aaaacaatgg gaaccaatga
    14101 ttttaaaatc atgttccgcg aaatcatgcc gaatctaagt tcattaatca ttactaattt
    14161 gacgatgaac tttgcggcaa acatcggtat cgagacaact ttgactttct taggtttcgg
    14221 cttgccggca aatgtaccta gtttaggtac attgatcggt tatgcaagta acggagaagt
    14281 cttagtgaat cggcaatgga tctggttacc cgcatcaatc cttattttag ttttgatgtt
    14341 gagtatcaat tatgttggac aagcattcaa gcgttctgca gatgcgcgcc aacggttagg
    14401 ctagtataga agcgaagggg gaaataggaa tgaagaaaac gatttttgga tttgcagcgc
    14461 tgatgtctgc tgtcgttctt gcagcatgcg ggggcggagg tacagatacc tctggaaaca
    14521 actctggagg caacggcaat aacaaagagg aattaacttt tccaattgca acaaaaaata
    14581 atgatgatgc aatcgaagac ggcgaattag aagctgcggt cgcaacggac agtcagttta
    14641 aaggattgtt ccagtgggag ttttaccaag attcatatga tgcagctttt atggctcctt
    14701 ctcatgaacc gctatttgca aacgatgacg atttcagaat cacagacgac ggagctgcaa
    14761 cgcttgatct agatgaagat acgaaaaaag cgacaatcaa agtaaaagac aacgtcaaat
    14821 ggtcagatgg tgaagatgta acggcggacg atgtgatttt cccttacgaa atcattggga
    14881 ataaagatta cactggtatc cgttatgacg acactttccg taacatcgta ggtatggaag
    14941 aatatcatga tggaagtgcc gacacaatct ccggaatcaa aaaaatagac gacaaaacag
    15001 ttgaaatcga atataaagaa gtaaacccaa gcatgcttca atcgggtggt ggtatctggt
    15061 catatgcagc accaaaacat acgttagaag gtgtagcaat caaagatatg gaatcaagcg
    15121 atcaagtacg taaaaatcca gtgacttttg gcccttatta catgagtaat atcgttgcag
    15181 gagaatcagt agaatacttg ccaaacgagc actactggaa tggtacgcct aaattgaaaa
    15241 agatcacgat gaaatctgta ccgatggcaa gtgcaactga agcattgaac tctaaattat
    15301 atgatatgat tttccaaatg ccaacagata cttacgatac ctataaagaa attgctggct
    15361 atacaaactt aggccgtgaa caaacttctt atacttatct aggattcaag ttaggtaaat
    15421 ggaatgcaga tacaaagagt gtggaataca atccagattc aaaaatggct gataaatcat
    15481 tacgtcaagc aatgggctat gcattagata ataatgcagt aggtgaacgt ttctatgcag
    15541 gtttgagaag caatgcgact tcattgattc ctccagtatt ccagtcatac catgatgatg
    15601 agatgaaagg attcacacaa gatctagaca aagcgaaaaa actattagat gatgctggct
    15661 acaaagatgt ggatgacgat ggtttaagag aagacaaaga tggaaataaa ctaacaatca
    15721 actttgcttc gatgtccggt ggggaaacag cgcaaccatt ggcagattac tatatccaac
    15781 aatggaaaga aattggtttg gatgtccaat acacaacagg tcgtctgatc gaattcaatt
    15841 ctttctatga taaattagaa aacgatgatc ctgaaattga tgtttaccaa gctgcttgga
    15901 atacaggaac tgatccaaat ccaagtggac tatggggacc aaatgctgcg ttcaactaca
    15961 ctcgttatga atcagaagaa aatacaaaat tgatcaacga tatcagttca agcaaagcat
    16021 ttgatgaaga gtaccaaaaa gaagcatatc aaaaatggca acaatatgca tttgaagaag
    16081 catttgcgat tccaacgttg ttccgtaatg aagtattgcc agtcaacgac cgtgtcaaag
    16141 actgggattg gtcttatgat gtaccaaacc catgggcaaa tgtttctgtt acagcagatt
    16201 cacgttcata atcactaaaa atgaccgaaa aagggcgatg tcctttttcg gttttttgat
    16261 atttttctgt ttatttctct aatattcagg tcaaggcgct gatttcatga cttctccaat
    16321 aaacggtgtt caaagtccag tttctcggta ttaagtcaaa tttatgcaaa aatatggaaa
    16381 gccattttca caaaattttt tcttcatact cgaaactaaa cgaatttttc acaacttctc
    16441 gtcaatcact aggattatta aagctttact taccttttga ccgaaccatt tcaaatttga
    16501 caaaaaacca gtataataag gaagtatcga aaaaatatcc ataaaaggaa tgtggaaaat
    16561 ggacaaccag ctgacaaaag aactaaaaga aaactataac atcgtttttc acgatctaaa
    16621 tttgcttgaa caagctttta cccattcatc ctatgtgaat gagcatcgca atctgcaatt
    16681 atcggataat gagcgacttg aatttttagg agatgccgtt ttagaactga tggtttctca
    16741 gtatttattc cgtttatatc ctcatttgcc agaaggaaaa ttgacaaaaa cacgagctgc
    16801 gatcgttcgc gaagacagct tgtcgaaatt tgccaaagaa tgtcactttg atcaatatgt
    16861 tttgttgggc aagggagaag agaattcagg aggacgtacg cgaccagcat tgctttgcga
    16921 cctattcgaa gcttttttag gcgcgttata tcttgaccag ggattcgaca cgacccatgc
    16981 attcatcgaa aaagtgattt ttcctaaagt caaagcaggt gctttttcac atgagatgga
    17041 tcataaaaca aaactacaag aagtcttgca aaaatcaggt gatgtatcaa tcgaataccg
    17101 attgatcaat gaagaaggtc cagctcatga acgagtattt tggatcgaag tttatgtaga
    17161 tgatcaattg attggcactg gacaagggaa atcgaaaaaa ttagcagaac aagctgcggc
    17221 ggaaaatgca ttggcagcac tgtaaaagta agtgagggaa agctttgtat ttgaaacgaa
    17281 ttgaaatagc cggttttaaa tcatttgcag acaagacagt cattgatttt gaaaatagcg
    17341 tcacagcagt ggttggacca aatggcagcg gaaaaagcaa tattacagag gctatccgct
    17401 gggtattagg agaacaatct gctaaaagtc tgcgaggcgg aaaaatgccg gatatcatct
    17461 ttgccggttc cgatacccgt aagcagctga atattgcaga agtcacagtg attttagaca
    17521 atagtgatca ttatctgcca ttggagtaca atgagatcag tgtaacccgc cgttatcgac
    17581 ggactgggga aagcgagttt ttcatcaata aacaaagctg cagattaaaa gatatccaag
    17641 aattgttctt agattctgga ttaggaaaag aatcgttctc aatcatttct caaggaaaag
    17701 tagaagcgat tttcagtagt aaaccggaag accgtcgtgg gatctttgaa gaagcagctg
    17761 gtgtcttgaa atacaagcag cggaaaaaga aagcagaaca gaagcttttt gaaacagaag
    17821 acaacctcag ccgcgtccaa gacatcatcc acgaattgga agaacagtta actcctttgg
    17881 ccgcccagag tgaggcagca aaggagtttt tacggctaaa agaaacactg actcaaaccg
    17941 atgtctcttt gatggttgct gaaatcaaga cagcaaaaaa agattgggac aacaaacaag
    18001 cacagcttgc gaaattcaat ctagaattag gcaaactatc tgagtcgatc caagaacaag
    18061 aatccatttt ggcgaagcag cgtaaagaaa atgcgcaagc cgatcgtctg atcgaaaaaa
    18121 atcagcaagt attattagat ctttcggaaa aactgaagca gacagaagga caaaaagatg
    18181 ttttgcagga gcggacaaag catacacaaa aaagctcgca agaatatcaa acttcattag
    18241 cagaagcaca aaaaaaggtt aaacattttg aaaagcttca agaaagcttg atgaaagcag
    18301 cagcagaaaa agaaacagag atccaaaaag ctgaagcaaa tctcataaaa acacagcaag
    18361 aattagaaaa ataccaaaaa tcaacgaaag aattattggc agaattaaga gatcaatatg
    18421 ttgatttgat gcaagaacaa gcagctgtag gaaacgaact gaagtatcta gaacgtcaat
    18481 atatacaaga aacagcaaaa agcaagcaga cgttagccaa gcaatcggaa gtagaagcat
    18541 cagtagaccg attgatctta caaaaagaag aattgacaca aaagcaggcg cagctaaaat
    18601 catctttaac tgaaacaaaa gagaagttag aaatgatcca acaaaacggg aaaaagttcc
    18661 aagaagcatt agcaaaagaa cagcctaaaa tgtatcaatt aatgaatcaa gtccagcagc
    18721 tacgtgctcg taagaaaagc cttcaagaaa tccaagaaaa ctattttggt ttttaccaag
    18781 gggttcgatt ggtcttgcag cataaacagc agctttcggg tattgtcgga gcagtggctg
    18841 aactgatcga tgttcctgca gatttcacgt tagctatcga agcagcttta ggtggggcag
    18901 cccagcatgt catcgttgaa aatgaaaaag atgctcggca agccatcacg tatttgaaac
    18961 agcaaagagg aggaagagca acattccttc cactgacgac aatcaaacca agacaattac
    19021 cggctcatat attgacacaa gcagcagctg tagaagggtt tatagggatt gccagtgaac
    19081 aagtcagtta tcccgatcaa atccaaactg tggttcaaaa cctgctagga acgatccttt
    19141 tagccaaaga cttaactagt gccaatgcaa tcgcgcaaac gatccgctat caatatcgag
    19201 tggtttcctt agaaggtgat gtgatgaatg ccggaggatc catgacaggt ggagcgaata
    19261 aacgaggaaa ccaaggcagt ttgtttgtac aaaatcaaga attgaaacag ttgactagtg
    19321 aatttgaaga agcagataaa caactccaag cacaagaaaa aaaagttcag gagctgcaac
    19381 aagaaacagc tcgtttagcc gaagaacaag aagttttgcg tacacgaggc gaacaattac
    19441 gttttgaaga acaagaagcg acaaatcagc tacaaaacat cataaatgaa cttgaacgtt
    19501 ttgaaaaaga aaagcaaatc tcaacttttg agacacgtga actgcagcag tttatcgaag
    19561 attacgaaaa gcaaacgaac gaattgaaag acaaacaaac agatcttgaa tcgcaacgac
    19621 aaaaaatcga cgaagaaatc aaatcgttaa gtcaagaaag cgaccagatg gaagccagaa
    19681 gagcacaagt acaaagtcaa aaagcacaag aacaagcaga tcttgctgtc ttgaaagagc
    19741 agtttaatca tctgcagatc cagctccgcg gtgcccgagt ccaaaaagca gaggcaaccg
    19801 aacgtcaaga agcgatagaa aagcagttgg cgacattgac agcagacttt tctgatcatg
    19861 aagtcacaga agaaagtttg gaaaaacaaa ttaacgaatt atctgctcag cgagaaacat
    19921 taaaagccga gttggcaaaa gccaaagagc agcgagatag aaggcaaaag gagatcgata
    19981 agcttgaagc agtcttggca gagcgtaatc gtgagcaaaa agcacgactg tcggaacaat
    20041 ccaaattaga agtccaaaaa gaccgagctg aaatgatttt agataatcat ttgacctatc
    20101 tgcagtcaga atatcagctg acatttgaga aagcttccca agactatcaa gaaacaaccg
    20161 atatcgaaga cagtagaaca aaggtttcat ccttaaaaga acagattgaa aaactgggac
    20221 cagtaaactt aaattcaatc gaacaatacg aacaagtcag tgaaagacat acattcttag
    20281 caacacagcg agatgatctg ttagcagcga aaaaccaatt gtttgaaacg atggatgaga
    20341 tggatgatga agtgcggaca agattcaaag aagtatttga agctatccgt caagaattca
    20401 aaattgtctt tccaaatatg tttggcggcg gtaaagctga attagtactg acggaaccaa
    20461 ctgatttgct gaaaacaggt attgagattg aagtacaacc tcctggcaaa aaactgcaaa
    20521 gtctaagttt gctttctgga ggagaacgag cacttacagc catcgcgttg ctcttttcca
    20581 ttatccgtgt ctgtcctgtt ccgttttgta tcttagatga ggtagaagca gcgttagatg
    20641 aagccaacgt gaaacgtttt ggtcgttact tgagcgattt ccaagatgac acacaattta
    20701 tcgtagtcac acaccgcaaa ggaacgatgg tcgcagcaga tgtactgtac ggagtgacga
    20761 tgcaggaatc cggtgtatca aaaatcgttt ctgtacggat ggaagatatc aatgaagagg
    20821 cgaaaatcaa cgaaacgaaa gtgtaggaac gaaaatgatc aaattagtag caatcgattt
    20881 ggacggtaca ttattaaatg aacaaaagct gatttcagat gagaataaac aagcattagc
    20941 acaagcaaaa cagcaagggg tcaagatcgt tctatgtaca ggacgcccct tagctgcgat
    21001 ggcgcattat ctgcaagaat taggactggt agatgaaggt gactacagca tcactttcaa
    21061 cggcggtttg gtacaaaaaa atgatactgg tgaaatcatg gaaaagaaag tgatggaagt
    21121 atcagatatc caacgcttat acaaactggc tcagcaattg gatctgccac tagatgtttt
    21181 gtctgacaat gtggttttac agctgcctag tgccccaagg aagcaatcct tgtacaatgt
    21241 gttgaataag ctgctgcaat tccaaccagc ggatataagc gatattacag atgaatttgt
    21301 gcttaataag gcagtaatcg cttatgacca agaagaatta gatcctaaaa tcaaagaaat
    21361 accagcagaa ttccatgaaa tgtatgagat catcaaaaca agaagtatgc ttttggaatt
    21421 tatgccaaaa ggtgttacaa aagcttatgg tatttcttta ttggcaaaag atcttggttt
    21481 gaaacaagaa gaaatcatgg cgatcggtga tgaggaaaat gatttaccga tgatccaata
    21541 tgctggacta ggcgtagcga tggagaatgc tgtgcctttc gtcaaagaag cagcagattt
    21601 cgtgacagcc agcaatgaag aaaatggcgt agccaaagtg atccaaaaat ttgtattaga
    21661 gtagggggag tcaaatggga ttttttgata aaatcaagcg tgcttttact ggcgaagaga
    21721 aaacagaaga agttgaagta caggagaagt acgacaaggg tttagaaaag acgcgtaaaa
    21781 catttggtga acggatgaat gagttgtttg ccaattttcg aacagtcgat gaagactttt
    21841 ttgaagaatt agaagaaaca cttatcggtg cagatgtagg ttttgaaaca gcgatacgaa
    21901 tcaccgaagc attacgtcaa gaagtgaaac tcagaaatgc gaaaaaatcg gcagaagtcc
    21961 aaaatgcgat catcgagaaa cttgtggatc tctatgaagc ggaaggaatc aatgaagtaa
    22021 atgaactaaa tatccaagaa gacggactaa cagttatttt gtttgtcggt gttaatggtg
    22081 tagggaaaac aacaagtatt ggtaaacttg cccatcagtt caaaaacgaa ggtaaaaaag
    22141 tattgatggc agcagcagat accttccgcg ctggagcaat cgaccagtta gttgtctggg
    22201 gcgaacgcgc tggtgttgaa gttgttcgtg gcaatgcagg aggcgatcca gcagctgtcg
    22261 tattcgatgc tttggaaaga gcaaagacag agcaggcaga tgttttacta gttgatactg
    22321 caggccgact gcaaaataaa gtgaacttga tgaacgaatt agaaaaaata aaacgagtaa
    22381 tccagcggga attaccagaa gcaccccaag aaacacttct tgtattagat gcaaccactg
    22441 gtcaaaacgc gatggttcaa gcaaaacaat tcaaagaaac aacagatgtg accggtcttg
    22501 ttttgacaaa attagatggt acagcaaagg gcggtatcgt acttgctatc cgtaatgaat
    22561 tgcatttacc agtgaaattg gtcggtcttg gtgaaggaat cgatgacttg gagccgtttg
    22621 atccaaatga ttttgtcgtt ggattgttca aaggattact aaaagaagaa taacccatgt
    22681 ctaaaaagca ggagctgtga caaaagttgc agctccttgt ttgaatgatt gtttttaaca
    22741 tagctgtgtt tttgatgcct tcatttttat acttatttgt aagcggttct caaacaattt
    22801 cgttcttttt cttagacttt ttgattataa ttagattcat atgaattgga ggtcacttaa
    22861 tgatgaattt taagcaatta ttattgcatg tcaatgcgcg cccttttatc gaccaagctc
    22921 gatttggtat agaacgggaa ggacagcggg ttgatcttgc aggaaatcta gcaaaaaccg
    22981 atcatccagc aatctttggc gatcgatcct atcaccccta tatccaaaca gattttagcg
    23041 aaacacaaac agagatgatc acccctgtta ccgattctat tcccgaatta tttcagtatc
    23101 tcgctgctgt ttatgatgtg actgctcgtt ctataccgaa agaagagatg atctggccat
    23161 taagtatgcc acctgcctta ccggaaaaag acgaagagat cattattgca aaattaaaaa
    23221 atttcgaaga tgtcttgtat cgacgttatt tagcaaaaga atacgggaag cgaaaacaga
    23281 tggtaagcgg tattcatttc aattttgaat ttggcgatga gttgctaaga acattgttca
    23341 gccatcagga agaatttcaa gatttttccg aattcaaaac agaactttat ttgaaaacag
    23401 cgagaaactt tatgcgctat cgctggatga tcacttattt attcggtgct tcaccaatga
    23461 gtgagaagaa ctacttttta gatgaatcac atccgcaaga acctgttcgc agtattcgca
    23521 acagtgcgct gggctatacg aatcatccaa atgtgaaagt ttcctatgct tctatgaaac
    23581 agtatttagc agatatcgag cgaatgatcg aagaagggaa actttcggag gagaaagaat
    23641 tttatacacc gcttcgtttc cgaggcggaa agaaagtcgc agatttagca acaacaggtg
    23701 ttcgttatat tgaattgcgg aatatcgatt tgaatcctta tgcaagattg ggtatcaatc
    23761 cagagcaagt tcggttctta caattattcc tgatgtacat gttatggaca gaagaaaaag
    23821 aagactgtga ccaatgggta gcagaaggta cgactcgaaa taacaaagta gcactagaac
    23881 aaccatcaga tcaaacagaa tttcatcaag aaggtagaga aattcttgaa ggaatgaagc
    23941 aaatgctggt tgaattggat tggttggatt ctctttattt agtagaggaa gcattgactc
    24001 aaatggatca tcctgaacaa acgttagcag cgaaactcta tcaagaagca cagctatcga
    24061 gtcagcaaga agttgctgtg gcattgggac atcaatatta taaagaaagc catgaacgtc
    24121 cctatcagtt ggccggtttt cgcgaaatgg aactttctac tcagatattt atgttcgatg
    24181 ccatccaaaa aggtgtccaa gtcaaagtat tagatgagtc ggatcaattt ttgcgtctgc
    24241 aattccaaga ccatgtagaa tatgtgaaaa atgcaaacat gacaagtaag gacagctata
    24301 tcgtcccact tatcatggaa aacaaaacag taacgaaaaa agtcttaaaa gaagctgggt
    24361 tccgggtacc aggaggcgca gaattttcat ccatggaaga agcggtaaaa gcttatccga
    24421 gatttgccga gcaggcattt gtgatcaaac ctaaatcaac caattacggg ttaggtatca
    24481 cgattttcaa ggaaggagct agtctggaag attatcaagc aggtttagcc attgcttttc
    24541 gtgaagacag ttcggtttta gtagaagaat ttatgccggg aacggaatat cgtttctttg
    24601 tgatcgacgg agaagtccaa gccatcatgt tgcgagtccc tgctaatgtt attggtgaca
    24661 gtatccgaac agtgaaagaa cttgttgaag aaaaaaatag tgaccctttg cgtggaacga
    24721 atcatcgtgc acctttagaa ttgatccaat taggtgaatt agaacaattg atgttgaaag
    24781 aacaagggtt gacgatcgaa tcagttcccc aagccaatca aattgtttat ctaagggaga
    24841 actcaaatat cagtacaggt ggtgattcga tcgatatgac tgatgaattt tctgaagctt
    24901 acaaaaaaat cgccgtttct gcggtagaag cactaggagc taagattagt ggtatcgatt
    24961 tgattatacc agataaagaa atcgatccta caactgataa aaaagcatat gggatcatcg
    25021 aagcaaattt caatccagcc atgcatatgc atgtttatcc ttttgcagga aaaggcagaa
    25081 gattgacgat gaatgtattg aaattgttat atcctgaagt attttaaaag aaaaagcttt
    25141 tgacagattg tcctatttga tgtcaatctg atcaaaagct ttttttgcat gtaaaaattt
    25201 atgtgttttt tcgttttaaa ataaacgatc ccagaagctt tttttctttt ttggtttttc
    25261 tttttcagct ggcgaagagt tttcttctgg gtattttttt tcgatatcaa tcacaggttc
    25321 atttactgct tcttttgctg ttaagagcaa tccgaattcc tcaggatttg cagtgacgta
    25381 atcgttgact acagtgaaac ggctattgtt tttcgttgct agctggatat attggttttg
    25441 gacagaaagg ggtagagccc cattcaagag tatggaagca tctggatgtt ttttgagttc
    25501 ttttaaaaag ttttctttgt tttttggttg gatcatttca gcaacagtca tactaagata
    25561 gcagcgttcc ctgaatgtcc ctaaatattt atgctgttct tcagggttca ctaaaggtgt
    25621 accataaagt cccttatcca aatggctttt tacatcatct tgtgccataa aaaataccct
    25681 ccttcttctc tttgtctttc ttacgattat agcatattcc caataatacc taaaacatat
    25741 gaaaaagcgg ttgcttttgt tcaacgattc acttaaaata agaatgaaaa tcattctcaa
    25801 ttagaaaggt ggattggatg cgctacggac gactagtagc attattagtt attttaagta
    25861 gtatttcatt gttcatcgga gtatcgaatg tacaattgtc agaactgctc cagttagatg
    25921 ggcaaagctt acaaatcttg ctagtgactc gtttgccacg aacgatttgt ttgatccttg
    25981 tgggagcgac cagtagtatt tgtgggttga tcatgcaaca tttgacccaa aataaatttg
    26041 tttcaccaac gactgcgggc acgatggata gtgcgcgttt aggtatttta gtggctatga
    26101 ttttcctacc tggtgcatcc ttgattgtcc gatccttgac agcattttgc tttgcttttg
    26161 caggaacatt gcttttttta tcccttacgc gattgttcct ccagaaaaat caattgatgc
    26221 ttccgttgat tggagtcatg ttagggaata tcattggttc agttgctact ttttttgctt
    26281 atcaattcca gttggtacaa aatatgactt catggcttca agggaatttt gcgactgtga
    26341 tacgaggaaa ttatgagtta ttgtatgctg ttgttccatt gttgctcttg gtttggctat
    26401 ttgcttaccg atttacgatc attggtctgg gcgaatcgtt tgcagtaaat ctgggaatag
    26461 attaccagaa gatgcagttt ttaggcattg gcttagtatc attggctagt gcagtcatgt
    26521 tgatcatggt aggaagcatt ccttttatcg gagtgatcat tccgaatctt gtctctagaa
    26581 tatatggaga tcaagtgcac aaaacaattg gcgtaactgc tttagcaggt agtttgttct
    26641 tagtattttg cgatcttgtc gctcgcgtgt tgatttttcc ttatgaaata ccagtcagtg
    26701 tgatcgttgg aatcatcggt ggggctatct tcctttatct tctgataagg ggacgacgat
    26761 atgcttaaaa aaaagccatc tatttttatt ggtatgcttg ttcttgtttt atttgttaca
    26821 ggaatgtact tgacgatcca tacatatgga aactggcatt ttgcatgtgc attgaggagt
    26881 aagaaaattt ttgcatttat ccttgtctcg ctagctgtca gcagtgccac aatcagcttt
    26941 cagacaatta cgcataacca gtttttgact ccttcagtat tgggacttga gtcgttatat
    27001 gtccttgtcc aaacggtcct tttcttcttt atcggaggag tgacgatgct tcagcagaca
    27061 ggtacagttt ttttcttatt gaacgttggg ctgatggtcg ggctgagtct attgttattt
    27121 ttctttcttt tgaaaaaaat gagagcgaat ctttttttat tactgatgtc agggatgatt
    27181 ttaggtacgc tttttggtag tgtcagtacc tttttacaag tcatcatgga tccgaatgaa
    27241 tatgatttgc ttcaaggcag attatttgca agctttggca atatccagag tcagtatctt
    27301 ttgcctgctt ctttgatcat tatcggctgc tggtacctgt tatttcgata tcgacatgtg
    27361 ctagacgttt tccatctcgg aaatgaacaa gcagtaaacc tcgggataga cacagatcgt
    27421 tttgaatttt tgattttatg ttgtgtcagt cttttgacag ggacatcaac ggctcttgtg
    27481 ggtccagtta cttttttagg gtttattgtc gcaaatatca gttatcgtgt cttttctact
    27541 tatcgtcatg gtcagctatt tatcggaagc tttctggtag ctatgctgtt tctgacagga
    27601 ggacagctcg cagtagagca gattttcggc tggaatacaa cagtgagtgt cgtcgttgaa
    27661 tttgtcggag gaatttattt tgtcacaaaa ttgattcgcg aaaggaagag tatcctatga
    27721 ttgatttaca aaaggtcaat aaactgtacc atggaaaatc agtggttaaa gatgtacagt
    27781 taacgattcc tgaaaagaag ttgactgctt ttatcggtcc aaatggtgct ggaaagtcaa
    27841 cggtgttgtc gatgatcagt cgtttgatac caaaagatac aggtgaaatc tatttagacc
    27901 ataacgaagt aaaggcttgg aaatccaatg aattagccaa aaaattatcg atcctacgac
    27961 aatcgaatga aataaatgtc caaatcaccg tgaaagaatt agtagaattt ggacgatttc
    28021 cttatacgaa aggaaaattg acgacaaaag acgaggaaat cgttcaggaa tcactggaac
    28081 acattgggtt agaagaaata gcggatcaat cgattgacac tctttcaggc ggtcaactcc
    28141 aacgtgcgta tattgcaatg gtgctagcgc aagacacgga ttatatttta ctggatgaac
    28201 ctttgaacaa tctagatatg aattttgcgg ttcaaatcat gcagatttta aaaaatttag
    28261 tacaagaaat cgggaaaaca attgttattg ttcttcatga tatcaacttt gctgcaagtt
    28321 atgcagacga aattgttgca atgaaagatg gacagttatt tacgcaagga acgacccaag
    28381 aaatgataaa aaaagatgta ttggatgaat tatacgaaat ggacatacgg atttgtgaga
    28441 ttgaaggccg cagattctgt ctttatttct aaaactaaca ttaggagaga agagatgaaa
    28501 aaattagtag taggtatctt gattctagca tcatttggtt tagcagcatg tggaaatagc
    28561 aacaacacca gtcaggcaga cacaaaaaaa agcagcacac aaactgaaac tacattgacg
    28621 ataacagata gtaatggaga ccaaatcgag gtgccaaata atccagaaaa agtcgtcgta
    28681 ttcgacaatg gttctttaga tacgatggat gctcttggtg ttggagataa ggtagttgga
    28741 gcagctacaa gcagccttcc tgagtatctt tcatcctata aaaaagtaga atcagcaggt
    28801 ggaataaaag aaccagactt agaaaaaatt aatcagcttc agccagatct aatcatcatt
    28861 tctggccgac aaagagattt tcaaaaagat ctaagtcaaa ttgctccgac cattttcctt
    28921 tctttagatg cgaaaaatcc atgggaatct ttccagcaaa atgtcactgc tttaggggag
    28981 atctttggta agcaagaaga agctaaaact caattagagg aattatctag tgcaatcgat
    29041 cagacgaaaa agaaagcaga agctactgac aaaaaagcac tagtcacact tgtgaatgaa
    29101 gggcaattat cagcctacgg atctggttct agattcggtt tcatccatga tttatttggc
    29161 tttgaacaag cagatgatca aatcgaagca tccactcacg gtcaaagtgt ttcttatgaa
    29221 tatgtgctgg aaaaaaatcc ggatatctta tttgtagtag acagaacaaa agctattggt
    29281 ggagacgaca gcaaagatga tatttctgcc aatgaactgg tagcacaaac gaatgctggt
    29341 aaaaatcagc agatcatctc ccttgaacca gatgtctggt atttaagcgg gggcggttta
    29401 gaatcaatga aactgatgat cgaagatgtg aatcaagcat ttaaatagca taaataacag
    29461 aggattaaac tctcatacta tgtaacaata atgtttctta cattcttaaa tagcaagagc
    29521 ctatcctcaa agaagttatc aaaaaccatc cgtaaaatcg tattgatgat tttacggatg
    29581 gtttttattt atttgagaaa tcttttttca acgtgaattc attatctaca tcatacgggt
    29641 tgattctaga atggggagtg gcaaaaaata gtgtgatcca ttttttcagc atcaagatcg
    29701 cctcctttga tttgaatcaa aggtacaaag cgaaacttag ataaaactta aatttagata
    29761 tttttatttt gtaccgatct cttctttaaa cgatgttcca tctgctgctt ctcggcaata
    29821 agtccactag actagaaaat atgagaagct attttctgtc cagcgttctt attactcaaa
    29881 acaaatcgca gttgtcacaa tctcttctaa acgatgttcc atctgctgct tctcggcaat
    29941 aagcccacta gactagaaaa tatgagaagc tattttctgt ccagcgttct tattactcaa
    30001 agcaaatcgc agttgtcaca atctcttcta aacaatgttc aatcagctga ccttcggtat
    30061 tagctcaaag cactgatttc acaacctcta tttaggcatg tgtctcagca agcggttgat
    30121 agttgatgat ctgcattttt cgataaaaat cggcgggaag cttactccac cgttttagta
    30181 attgatagaa ataactctct tctgctttga ttttatactc gcttaatatc aatccaaaac
    30241 tattcgtttt ttttgaagct aattttttat aaatatccaa tgctttggtt ttatcttcat
    30301 tgacaaagag tgtttttact tcaatataag aaccctcgat attttgtgtt tcttcgataa
    30361 aatagatgtt ttttatatat tccttcatag tgattcctcc aaagaagtct tttatttgat
    30421 aagtttactc taccgaagag ttgttaataa tccgtgaaca aaaagtaatt ctttttccaa
    30481 tagttgttaa aggtttttta ttttttatag tattttttgt tatttaagaa aggaataagg
    30541 ttaaacaaat aacaggttgc tttttgggtc tttttccgtt caagaaactg aacgaagaaa
    30601 agggaaagtt gtctcttttg tgataagaga aatcataata aacgaatgac ttactttcca
    30661 ataaattgag aacagtcaga accagacaaa ttagttgcat ctgatgaata aagtgattat
    30721 tctatgacat gaggtgatac agatgaaatt agaagtgaca aaagaagcac aagaagtatt
    30781 gaagaataaa ttagaagcag acgatcaact tttattagat atcgaaaacg gtgacggtcc
    30841 atttgcagaa agccaagtca gttgccaatt ggatacggca tttcgattga tcattgtcaa
    30901 aaaagataca caaacagatc taagtcttta ttctgtagtg gctgatacac cagttggacc
    30961 aatcaaaatc aaagacagtg caatacttta catggatgat ccatccacac ttgctttaga
    31021 accaacttat aatagtttac aattaaaagg accaagcggc ttgagaaaag gcaacttgca
    31081 agttgttcga aaagactaga gggataaaat gggaaaattg gcgatcatca gcgacctaca
    31141 tgtggacatc aataaattcg gcgaatcaga gctgatgcag ctttggcaag ttttgcaaaa
    31201 gcacaagatc actcggcttc atctagccgg agatacggct aataaagtag accgttgcct
    31261 agctgttgtc gaatttttcg tcaataaagg attatctacg acatttcatt tcggcaatca
    31321 cgagttggca gatgtgccca atgaacagat gatggaccat tttccagacc cacatttttt
    31381 aaatgaacgg tatattgctt taaatgagca gaccgttctt ttagggatga atggctggta
    31441 cgactatcag ttttccgaat tgaaagatta cgaggcaatc gtacgaatga agcgtttgta
    31501 ttggtatgat cggttcatcc agcgtgggaa aacagatctc gaggtcaatg cagaaatgct
    31561 tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa aaacttgagg tgattttatc
    31621 cactcatttc gttcctaaac gggcatttat catgtatcaa tctgctcctt atgagcgttg
    31681 gaacaaatta aatgcatttt taggatcaga atcattcgga gaattacttg accgttattc
    31741 aaatatcaaa caagtagttt ttggccatac gcatcgtcgc tttgaagata aatccatcgg
    31801 tcacacgatt tacagctgca gaccttttgg ttattattat gaatggcagc tgacccgaga
    31861 ttttgtcttg aaagaacagt tgctagacag ctatgatcct atgaaattac gtgtcctctt
    31921 gcgtgcccat cacccagctt ttcaggccta tcaaacacaa catttgcaag aggaatttga
    31981 acaagcgatg acaatcgttc cgtattgaga cagcattagt aatgtgtaaa agaaaatacc
    32041 ttgtaaaaca taaggataag taagagcagt agatccattt atcgtggata tgctgctttt
    32101 tttgcggaaa ataatcagcg aatgtttatt tgatcggatg atttgtaact cgcagagtac
    32161 tagatgcaag cgtgtattct ttttttgaac gataatcaca aaaaaaaata taatatacat
    32221 tttttattta attataatta atattgattg aagtctaggc agtcaagt
//
LOCUS       DAZKMB010000017        31690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000017 DAZKMB010000000
VERSION     DAZKMB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.017"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1488)
                     /gene="cls"
                     /locus_tag="ACG8V3_000746"
     CDS             complement(37..1488)
                     /gene="cls"
                     /locus_tag="ACG8V3_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0299309.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1829..4495
                     /locus_tag="ACG8V3_000747"
     CDS             1829..4495
                     /locus_tag="ACG8V3_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0299310.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4859..5611
                     /locus_tag="ACG8V3_000748"
     CDS             4859..5611
                     /locus_tag="ACG8V3_000748"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0299311.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            6064..7452
                     /gene="ltrA"
                     /locus_tag="ACG8V3_000749"
     CDS             6064..7452
                     /gene="ltrA"
                     /locus_tag="ACG8V3_000749"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0299312.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(7574..8869)
                     /gene="purB"
                     /locus_tag="ACG8V3_000750"
     CDS             complement(7574..8869)
                     /gene="purB"
                     /locus_tag="ACG8V3_000750"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0299313.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(8882..10006)
                     /gene="purK"
                     /locus_tag="ACG8V3_000751"
     CDS             complement(8882..10006)
                     /gene="purK"
                     /locus_tag="ACG8V3_000751"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0299314.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(9999..10487)
                     /gene="purE"
                     /locus_tag="ACG8V3_000752"
     CDS             complement(9999..10487)
                     /gene="purE"
                     /locus_tag="ACG8V3_000752"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0299315.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(10903..12210)
                     /locus_tag="ACG8V3_000753"
     CDS             complement(10903..12210)
                     /locus_tag="ACG8V3_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0299316.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(12213..12794)
                     /locus_tag="ACG8V3_000754"
     CDS             complement(12213..12794)
                     /locus_tag="ACG8V3_000754"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0299317.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(12910..13007)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(13366..14658)
                     /locus_tag="ACG8V3_000755"
     CDS             complement(13366..14658)
                     /locus_tag="ACG8V3_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0299318.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(14655..15278)
                     /locus_tag="ACG8V3_000756"
     CDS             complement(14655..15278)
                     /locus_tag="ACG8V3_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299319.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(15281..16117)
                     /locus_tag="ACG8V3_000757"
     CDS             complement(15281..16117)
                     /locus_tag="ACG8V3_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0299320.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(16134..17021)
                     /locus_tag="ACG8V3_000758"
     CDS             complement(16134..17021)
                     /locus_tag="ACG8V3_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0299321.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(17037..18320)
                     /locus_tag="ACG8V3_000759"
     CDS             complement(17037..18320)
                     /locus_tag="ACG8V3_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0299322.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(19003..19686)
                     /locus_tag="ACG8V3_000760"
     CDS             complement(19003..19686)
                     /locus_tag="ACG8V3_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0299323.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(19704..20321)
                     /locus_tag="ACG8V3_000761"
     CDS             complement(19704..20321)
                     /locus_tag="ACG8V3_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0299324.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(20331..21155)
                     /locus_tag="ACG8V3_000762"
     CDS             complement(20331..21155)
                     /locus_tag="ACG8V3_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0299325.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(21148..21708)
                     /locus_tag="ACG8V3_000763"
     CDS             complement(21148..21708)
                     /locus_tag="ACG8V3_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299326.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            21882..22838
                     /locus_tag="ACG8V3_000764"
     CDS             21882..22838
                     /locus_tag="ACG8V3_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0299327.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(23143..24099)
                     /locus_tag="ACG8V3_000765"
     CDS             complement(23143..24099)
                     /locus_tag="ACG8V3_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0299328.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            24203..24805
                     /locus_tag="ACG8V3_000766"
     CDS             24203..24805
                     /locus_tag="ACG8V3_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0299329.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(24893..26392)
                     /locus_tag="ACG8V3_000767"
     CDS             complement(24893..26392)
                     /locus_tag="ACG8V3_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0299330.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(26408..26932)
                     /locus_tag="ACG8V3_000768"
     CDS             complement(26408..26932)
                     /locus_tag="ACG8V3_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0299331.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            27230..27979
                     /locus_tag="ACG8V3_000769"
     CDS             27230..27979
                     /locus_tag="ACG8V3_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0299332.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            27970..28755
                     /locus_tag="ACG8V3_000770"
     CDS             27970..28755
                     /locus_tag="ACG8V3_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0299333.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            28724..29440
                     /locus_tag="ACG8V3_000771"
     CDS             28724..29440
                     /locus_tag="ACG8V3_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0299334.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(29542..30561)
                     /locus_tag="ACG8V3_000772"
     CDS             complement(29542..30561)
                     /locus_tag="ACG8V3_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0299335.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(30580..31581)
                     /locus_tag="ACG8V3_000773"
     CDS             complement(30580..31581)
                     /locus_tag="ACG8V3_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0299336.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         9647 a         6552 c         5850 g         9641 t
ORIGIN      
        1 aatcttagta tatttttctc tacttgaata aaaaaattat aggattggag aaaataatcg
       61 agaaatttgt tgcttgaata atagccagtt agacatttcc ttgactgttt cagttgtcat
      121 ggatgaacaa tgtgtctcat cttctttgaa ctgagtagca agttcttcta aaaactcagg
      181 atcgtaaatg acagcacttg tttcaaaatt caatcggtag ctacgaatat cttgatttgc
      241 tgaacctacc atgcagattt catcatccat tatcaatgtt tttgcatgga ggaagccgtt
      301 ctcatagaca aggatttgta cattttcctt gatcagctgc cgagcataat attgtgttgc
      361 tcgataaata aaaggatgat ccggtttgtt gggaatcatg attttcacat ctacaccaga
      421 ggcagttgcg atttttaaag cagcaatgac actttcatca ggaacaaggt aaggtgtctg
      481 tatccaaaca cgttttttag cagaagtaat caatttgata aatgaaagct tgatttgttc
      541 cctttcgtta ttcggtccgc ttgaaacaag ctggatggat gtatcagcaa gcttagattc
      601 atctcgatct gcttttttaa agaaataatt caaatgatag ccgacctttt tttcttcagg
      661 taccgagacg ttccagtctg ttaagaagcg aagctggagc aaagaagaag cggcaccgaa
      721 aatccgtata tgcgtatcgc gccaatagcc gaattttttt gttgtattta catattgatt
      781 ggcaatattg aaaccaccgg tataactaat ctttccatcg atcacaacga tttttcggtg
      841 atcatggtaa ttcaatcgga aacgaagtaa tgccctttgt gaggtaacaa atgtatggac
      901 atgtccacca tttttgatta gacgattgaa atcttttgct tttgtgccat gagagccaaa
      961 tgcatcatat aatattcgaa cttccacgcc ttctgcggct ttttcttcta aaacatgtaa
     1021 gacttgctgg ccgatattat ctgtcacaaa tgcgtaatat tcgatatgga tcgaatgttt
     1081 ggcttttttt agatcttgaa gcagtgaatc caatttctct tgtccgtctg tgagaagagt
     1141 gacagaattc attcttgtca gcggcatacg atttaatgaa gagaagaagt caacaaattg
     1201 ctgtttgtct ttatcgccca tgtcaggatc gtaatgttca atggtatctc ctctaaaaga
     1261 ctgaaagttt tctaattctt tcaagtcact ttgtcggaga tagaattttt tcttatccgt
     1321 taatccacgt ccgaaaaata aatataatac aaagcccacc ccaggaaggg caaacaatac
     1381 taagagccat gcccaaatgg ctgctacatc tcggggtttg atcaaaatag ccaccaaagc
     1441 aataagtgca tttaatagat aaagggcggt tataatacta gataccacaa aaatgttcct
     1501 ccttacatac aataacatta gtatagcatg atagaagaaa gaagcctacc aaggtgaata
     1561 tacttttcac tcctttcatg aaaatgtagg taaatcgggc taaagtagtt gctttttgta
     1621 ggttaataag gtaggatatt agtacagttg aatatctcct taggggagta accggcaacg
     1681 tttgttgcga taatatcaac agtaagttaa ccaaatggtt ttctggtatt gtatgaaatg
     1741 gtgagaccta agacaaatgc gtatttgtct taggtctctt ttttttcaga caggactagg
     1801 gccaagatca aaaaatgggg gatgaaattt ggaaaacaag gaacaagcac aacaagcggt
     1861 ctataaagaa acgatcgact cgttgatgca gcaaacgcag agctcaaccg atggattgag
     1921 caccacgcaa gcgaaggaaa aattagaaag agatggcttg aaccagctaa aagaaaagcc
     1981 gaagaagcca acttggaaat tgttcttaga aactttcaaa gatgcaatgg ttatcgtatt
     2041 gctgattgtc gcactgattc agatggtcat gggtgactac atcgaattct tagttatttt
     2101 tgcagtattg atgttgaatt ctataatcag tgttgtccaa acaaaaaaag cagaaggttc
     2161 tttagaagcg ttgaaaaacc tttcagcgcc cgatgctcga gtgattcgtg atggtaagga
     2221 aaaaacgatt ccggcaaacg aacttgtggt aggagatatc gtgttgttag acgcaggcga
     2281 ttatgtacct gctgatggac gtttactaga tgcgggatca ttaaaagtag atgaaggtat
     2341 gctgacagga gagtcgactt ctgcagaaaa agaagtaacg gatatcacaa gtacggtacc
     2401 aatcggagat cgtacaaata tggtattcag tggaacgatc gtaacttacg gacgcggtaa
     2461 attcttagtg acttcaactg ggaataatac ggagatcgga caagttgctg gccttttgga
     2521 atcaacaact gagcaagtca ctccgttaca acgaggtctg gataaattca gtaaaaaact
     2581 aagtttggct attttggcat tgtctttatt gattttaggt atccaactgt ttcgtattta
     2641 tcttggagaa ggtacaggag atatgactgc agatatcgtc agcgcagtga tgtttgccgt
     2701 agccgtagcc gtagctgcta ttccagaagc attacagtct atcgtaacaa tcgtgttatc
     2761 tttgggaacg aataaaatgg caaaacgtca tgccattatt cggaagcttc cagcagttga
     2821 aacacttggt tcgacaagta tcatctgtac agataagact ggtacattga cacaaaacaa
     2881 aatgacagtt gttgactact ttttaggtaa tggcgatact ggggatttca caaatgaccc
     2941 ttcaaaatgg acagctgacg aacgtcgctt gatcgaaatc agtgttttag cgaatgatgc
     3001 atcgatcagt gaagacggta ctaaattggg cgaccctacg gaagtagcgt ttatcgactt
     3061 tagtgaaaaa ttaaaccagc cttatcagga aatccgaaac aactacccaa gacaagcaga
     3121 acttcctttt gactcagatc gaaaattaat gtctactgga cacacaattg ataacaaaaa
     3181 ttacttgttt gttaaaggcg gaccagatat cgtcttccaa cgcagtaaaa aagtattgct
     3241 aaatggtgaa gtcgttccga tgacggatga attgatgaaa aaattccaag agcaaaatga
     3301 agctttttca acacgcgcat tacgtgtact agcttttgct taccgtccac ttgataattt
     3361 tgatttatca tttgaagatg aagacgattt gatccttgtt ggtttgcttg ctatgatcga
     3421 tcctccacgt gctgaagtga ctcaggcggt aacagaagca agaagtgcag gaatcaaaac
     3481 aatcatgatt actggtgacc acaaaacaac agctgttgct atcgcaaaac aaatcgggat
     3541 ttttgaagaa ggaaacctgg ctttgaccgg gacggaacta gatgaattga cagaagatca
     3601 actaaacgaa gttttagaaa aagtcactgt ttatgcacgt gtttcacctg agaataaaat
     3661 tcgtatcgtt cgtgcttggc aaaataaagg tcatatctca gctatgacag gtgatggtgt
     3721 aaatgacgca ccagcattga aacaagcaga catcggaatt gctatgggaa cgggtacaga
     3781 tgtggcaaaa gatgcagcag cgatggtatt gacggatgat aactttgctt ctattgtcag
     3841 cgcagtagaa gtaggacgta atgtgtttga taatatcaag aaagctattt cttatctatt
     3901 tgcaggaaat ttaggtgcga tcattgcgat catctttgcc ttagttgctg actggtccaa
     3961 tccatttaca gcattacaac tgttgttcat caatttagtc aatgattcac tgccagcaat
     4021 tgcattaggt acagaaaaag ctgaacctgc tacgatgaac cgcccgccaa gagatccaaa
     4081 ttcaggaatc tttacggaca aaacattgat ttctgtaacg tatcgtggtg tgctgattgg
     4141 gattgctgtt atctttgctc aatggcttgg catgcagcat tcacctgaat tcggtgtagc
     4201 catgtcattt tcaacattga tttggtgtcg gacattgcaa acattcccag cacgttcaaa
     4261 cacacaaaca gctatccaag cagggttctt ctctaacaga agtgtcttac tagctgtagt
     4321 tgtatgtagc gcgctttact gcttaacaat gatcccagga ctgaaagaag tcttctctat
     4381 cccatcagca tttggattga cagaagtctt tacatgtatg ggaatcgcac ttgtgacaat
     4441 tgttttgatg gaaatcacta aattgatcat tgtacgagta caaggaaata aataaaacga
     4501 atcaacgagg ttgtgagcct gccgtttaat tctgagtcac aaggtgcaat ttcaacaaac
     4561 agcttctcat gtttgttgaa tattgtgact tgtgaccgaa gagttggcag gagaacaccg
     4621 tttaaggaag aagttgtaaa atcagcattt tgagctaata ctgagggtca tctgattgga
     4681 cactgtttat tcggaaagag ggactttgac acgactttgg tcacagtccc tttttttatt
     4741 tatgatagac ggaaagccaa tcattttatt attcatcaaa agagatagaa tagataaaag
     4801 aggagaatga tttattccga aaagtgaaaa catataacaa aaaattagag gaaatcaaat
     4861 gaatgaaaaa gaatgggatg aatttgctga agaatattat gaaatccagc aagaatcgca
     4921 agtaactatt gctgaagatg taaaaaatta tctgaaaacg gagaaagtcc tgcctgctga
     4981 aacagcagca gacgttgctg gaggatctgg cagatttctt tccttagcaa aagaaattaa
     5041 tcgctatgat ttgatcgatt tttcttcagg aatgctagaa aatgcaaaaa aagaagcaga
     5101 aaaaaatgag ttgccaaatg tttcctttat caaacagtcg ttagaagaat ttatccaatc
     5161 aaatgagaca tatgaaatgg tttttacagc agcaaatcca gcattggtac atcttattca
     5221 attagaaaaa ttgatgaaaa agtcaagcaa atggtgcgtg ataatcagag tagtggaatc
     5281 aatcgatgat gttttttatc ctatcgaaaa gcaactagga atgatagaag aagatcctaa
     5341 tacatcgcca gttgtaatgg atcaatttga aacatactta attgatgaag gattcaaagt
     5401 gaagaaaaaa gattttactt attttacaca ggaatcaatt actgaagaat tgatgaagct
     5461 atactatgac gcgtacgcaa aagatcctgt tgtgaccagg tacattcgca aactattcag
     5521 tgaaaaacga gaaatcctta gtacgactaa gctgacctat agactgctcc tttggtccgc
     5581 agataaaaaa agaacctttc acgactcgtg aagggttctt tttatgatgt gcgccctgca
     5641 tgggtgaaaa cttggtggtg aaagtccact acaggcttgg cagtaggaac tgttagcgaa
     5701 agacaaggcg gcaatcgtga ggttgtggct gaaggaagtc ggacgcaaac tcctgaactg
     5761 acgaacagaa actagataca aggctgaatt aggtcggata agtcttcatc acaagacaaa
     5821 gtccaatact gcccgaaacc tatacagtaa atctagcagt tacatgggag gaaagttttc
     5881 actcttaccg ggggagatct catgagggtg ttgtgggcgt tgaatgaaaa caaaccagca
     5941 caacgatcat ggcgacatga tggtgaatca tgagaagtca gccgaggtca tagtagtaac
     6001 gacactgacc gttacgaagg actgaacaat aagaatcttg aataatttag gaggtgtgag
     6061 aagatgcgac gaccgcagaa aacaagaaaa cttggctatc atcaaagagc tagtgtggaa
     6121 cacgaagggt atgatgaagt gcgcagcact tcttgcggtg aacagaggaa acaagatggt
     6181 gtagaaaatc tgtttgggag aattctagat cggaataatc tcaatcaagc gtatcttcaa
     6241 gtagtcagaa ataaaggggc ggctggtgca gatgggatga ccgtcgatca actgcttcct
     6301 tacttgaaag agcaccgcga ggaattacta tccaatttac gccaagggaa ctataaacct
     6361 cagcctgttt tgcgagtaga aataccgaaa ccaaacggcg gtgtaagaaa actaggaatt
     6421 cccacggtta ttgatcgtat gcttcaacag gcaatcaatc aagtgttaca accgctattt
     6481 gaaccagaat tctcagataa tagttatggg tttcgcccaa atcgcagcgc acatgacgca
     6541 gtgaagaaag caaaatctta ttatgaacaa ggttaccgat atgtggtgga cattgatatg
     6601 aaagcctact ttgacaccgt aaatcacgat aagctgatgt atttcattga gcggaaagta
     6661 aaggacaaac aagtcctaaa actgatccgt caatacttaa tgagtggtgt aatggaaaat
     6721 ggactcatca caacaactga agaaggaacg ccccaaggag gcaatctctc tcccctgtta
     6781 agtaacatct acctgaacga atttgatcaa gtgctagaaa atctaggaca taaatttgtt
     6841 cgatacgccg atgactgcaa tatctatgtt agaagcaaac gagcaggaca gcgtgtgatg
     6901 aagaaatcca ttcaatttct cgaagaacaa ctaaagttga cggtaaatcg agaaaagagt
     6961 gcggtaggaa gtccattgaa acgaaagttt cttggattct gcattctgcc aacaaagaaa
     7021 ggtattaaaa ttcgccctca cgcaaaagca aaacagcgtg tgaaaagcaa gctcaaacaa
     7081 ctcactaaaa gaaaccgtgg aagaagtatc caacttattt taaaagaaat caagcaacta
     7141 atgactggtt ggatcaacta ctatggaatt ggtgagatga aagaattcat gagggagtta
     7201 aacggttggt tgaagcgaag aatcagacaa tatatttgga aacaatggaa gaatccaaga
     7261 accaaaagaa aaaatctgat tcgtttaggg atcgataaag cgaaagcgta tgaatggtca
     7321 aatacaagaa aaggtatttg gtcagtctcc aaaagtcata ttcttcatcg atcattaaca
     7381 gacaaagaac tggcccgcca aggatatgag gacatctcct tgaagtacca gttcgtacac
     7441 tcaacttatt gaaccgccgt atacgggtcc gtacgtacgg tggtgtgaga ggggcggaag
     7501 gtcagttggc tttcccctct actcgattat ttttgctatt ctattttaat tgaattttgc
     7561 aactcactct tctttattcc aaacctactc gtttgaatat cacgtcaacg tttttcaaat
     7621 gatgatgata atcaaaggca tcgtctattt cttctttaga taataccgat gtgatggttg
     7681 gatcttcttc taacagcggg cggaactgca cttggttgtc ccaagcgtac gcagtttttg
     7741 gctgaacaag atcataagct tcttctcttg tcattccctt atcgatcaat ttcagcataa
     7801 cgcgctggct ataaatcaaa ccaaatgttg cagacatatt ccgcttcatg ttttcaggaa
     7861 agaccgttag atttttaacg atattgccaa aacgatttaa catgtaattc aataaaatcg
     7921 tggtatcagg caagatgatt cgttctgctg aagaatgaga aatatctctt tcatgccata
     7981 atgtgacatt ttcataggca gtgaccatgt gtccgcgcac cactctggct agaccagcca
     8041 tattttctga accgatcgga ttccttttgt gcggcatagc agaagaacct ttttgacctt
     8101 tcgcaaaaaa ttcttctact tcacgtgttt cggatttttg caatccgcgt atctctgttg
     8161 caaaacgttc aatacttgta gcaataagcg ccatcgctga gaaatattca gcatgtagat
     8221 cccgagggag aacttgagta gagatttctt gtggacggat ccccaattgt ttacatacat
     8281 actcttcaat aaatggcggg atatttgcga acgttcccac tgctcctgag atttttcctg
     8341 cttctactcc ttttgccgca tgttcaaatc gttcgatgtt ccgtttcatt tctgaatacc
     8401 atgtggcaag tttcaatcca aaagtagttg gttcagcatg gacaccgtgt gtccgaccca
     8461 tcatcactgt atatttgtgt tcttttgctt tttcgccaat gatttccgta aaacgtttca
     8521 agtcttcccg taaaatatca tttgcttgct tgatgagata gccataagca gtatctacga
     8581 catccgtact tgttagacca taatggaccc attttctttc ttctcccaat gtttcagaaa
     8641 ccgctcgtgt aaacgcaacg acatcatggc gtgttaattt ttcgatttcc aagatccgat
     8701 cgatatcaaa tgatgcatgc tcacgaattt ttttcacatc ttcttgcggg atttcaccaa
     8761 gttctgccca agcttcatct gctaaaatct cgacttcgag ccacgcacgg tatttgttct
     8821 cttccgtcca gatattcccc atttcagggc gtgtataacg ttctaacatg ttttttctcc
     8881 tctaatccca aatattcgtt tgtttgattt cttccagcgt ttgctggatg tcttctgtca
     8941 agatagtgat atgtcccatc ttgcgacctt tttttgcttg tttttttcca tagaaatgga
     9001 attgccagtc tggcttattc tcaatcaagc gatagctttc cagcagttgt tctcccaaaa
     9061 cattgaccat tactgcattg cttatcaaac gtactttttc tcctaatggc caaccacaaa
     9121 tacctcgaat atgggcatcg aactgactca tagaacaagc ttcaatagaa taatgtccag
     9181 aattgtgagg acgaggagct aattcattca catatagccc gcctgttttt gttaaaaaca
     9241 tttctacccc taaaacacct gaaaggctca ctgcttctgc aatcacacgg gcaatacgct
     9301 cagcctcctc gatcacatcc tgatcaatcg cggctggagc aatcgtttca tgaagaatat
     9361 tgttgtggtg gatgttttcc acgaccggaa aagtcgtgaa atctccttga ccatttcctg
     9421 ccaccatgat cgaaatctct ttttcaaacg ggatccatgc ttctaaaaca caggttcctt
     9481 ctcttaaaag gtccatggca ggagctagat ccgcccgact tttcaaaaca tactgcccct
     9541 tgccatcata gccgcctcga gtggttttca aaacacaagg atagccgatc ccgtcaatcg
     9601 catcttggat atccgtcgga ctgacgatcg ttgcataagg agcaatcacg atgttattcg
     9661 tttccaaaaa agatttttct aacaggcggt cttgcgtaat cgcaagcaag tctgtccctt
     9721 gaggaataaa tgacatgggt aaaatcgcat ttaacgcctc tacactgacg ttttcgaatt
     9781 cataggtaat gacatctgtt cttctggcaa gctctttcag cgcaaaggtg tcatcataga
     9841 cactttcaat gtgccaatct gcaacttgtg cggctggaca atcgctcaca ggatccagca
     9901 cacctacttt aaagcccatt tcttttgcac taatcgccat catacgacct aattgaccgc
     9961 caccgacgat cccgatcgtt gaaccaggca acaaaggttt aaccaagttg atcactactt
    10021 tctataacag ttttagccaa cattgaacgt ctttcagcca atttagcctc caattctaaa
    10081 tcatacatgg aaagcatctg tatcgcaagt agtccagcat taattgctcc cgcttttccg
    10141 attgctgtag tagctacagg tactcctcca ggcatttgga caatcgaaag aagcgaatcc
    10201 aaaccattta atgttcgtga ttggaccggt acaccgataa ccggcaaagt tgtttttgat
    10261 gcgaccatac ccggcagatg cgcggctccc cctgctcctg caataatgat tttgattcct
    10321 ttttttcgag cgttttgtgc atattcaaac atcaaatctg gggttcgatg agcagaaacg
    10381 accttttttt cataagacac accgaattca tccaatattt gacatgcttc tttcattgtt
    10441 tcccaatctg aagtactccc cataatcaca gagatttttg ctgtcattgt ttttcctccc
    10501 agtttttcta cattcatatt ttaacaatca ctttttttga tgtcaaagaa atttcgaatg
    10561 ttaacaaaga aaaacatttt attgttcgtg tttattaaag ataaaaaaaa ggaatgtgac
    10621 aaaagttagg tcacattcct ttttatggat aaacggtgtt ctcctgccaa ctcctcggtc
    10681 acaaggaaca atattcaaca aacatgagaa gctttttttt gaaattgttc cttgtgactc
    10741 ggagatgaac ggcaggctca cgacctcttc cttcaacggt gttcaatcag ctgaccctcg
    10801 gtattagctc aaacaaacgg caaaatacga ggaactattt ttccgtttgt ttctctaaca
    10861 atcaggtcaa aacgctgatt tcacaacctt tcttttgctc tattacttct tgaaggcttc
    10921 ttccaatatt tcttcatctt ttttcaatcc attaaagatc aaattcaata tgatagccgt
    10981 aatgctactc attacgatgc catttccagt gaacatttgc aatgtttgcg gcatttgact
    11041 gaataatgtc ggcgtaacat tgaaacccag cccaaatcca acagaaacag caacaatcaa
    11101 tagattttta tcattgctga aatcaacttc caacagcata cggattcctt ggactgccac
    11161 catcccgaac atgactaaca ttcctccacc aagtaccggc tcagggatga tttgtgctaa
    11221 cgcaccgatt ttagggaata atcctaaaac aatcaagaag aatgcagaga aatagatggg
    11281 ttttcttgtt ttgattcccg aaagttgaac caatccgaca ttttgagaaa agccagtata
    11341 agggaatgta ttgaaaatac cgcctaagat aacggcaagt ccttctgcac gatatccctt
    11401 tttcaattct tcttctccaa catttttccc agtaatgtca ccaagtgcaa aatatacacc
    11461 tgttgattct accatgctga ccatggaaat gatgatcatt agtaaaatcg accatacgtc
    11521 gaaagttggt ttaccaaaat aaaagggttg tggaacatgg aacaacggcg catctccaat
    11581 cgcgctcaga tcaatcattc ccatgaaagc agctaagatg cttcctccga ctaaaccaat
    11641 caataccgcg attgaacgaa tgaacccttt tgccagcatt tgcacgccta caattaggag
    11701 aatcgtcaca aatgcaagta gcagatttgt tttgtcacca aaatcagctg ctgttgcatt
    11761 acctccaccc attttctcga ttgctacagg aatcaaagtc aaaccaataa cggtgataac
    11821 cgttcctgtt actataggag ggaatagttt tttaatttta gaaaagacac cagaaactaa
    11881 cacaacaaag atcccagaag cgataataga tccgtagata gctcctactc cttcattggt
    11941 tccgatcaag atcagtggag ctactgcttg gatcgcacat cctaaaacga caggcaagcc
    12001 aattccgaaa aatcggttaa ctgtcaattg caataatgtc gctaatccac acataaagat
    12061 atcaattgaa atcaaataag tcatctgttc ttgattaaag cctagtcctg taccgatcaa
    12121 aagaggcacc gctactgctc ctgcatacat cgctagcaga tgctgcagcc ctaaaaccgc
    12181 agcttttcca ttttgtgttt ctttttccac gattatgcgt cctcctcaag aaactcaact
    12241 tgtccattcg tcaaggaagc aatacgcgca agagaaacga ctctcaatcc catttcttca
    12301 agtaactgac gtccatcttg gaaagacttt tcgatgacga tcccgattcc ttctacttgc
    12361 gctccagctt gctggcataa ttcgatcaat ccttttgccg cttgtccgtt tgccaagaaa
    12421 tcgtcgataa tcaatacttt gtcttctgaa gataaaaatt tacgagaaat agaaatcgta
    12481 cttgtcactt gtttcgtaaa agaataaaca gaagctgtca ataattcttc atccatcgtc
    12541 aaactttttg cttttctagc gaagatcatt ggaacatcta attctcttgc agcaaataaa
    12601 gctggtgcga tcccagaagc ttcaatggtc acaactttcg tgattccttg ttctttaaat
    12661 acttctgcaa accgttttcc catttgttcc atcaatacag ggtctacttg gtgagtgacg
    12721 aagctgtcga cttttaatac gccctcgcct aatatacgtc cgtctttttg aatacgttca
    12781 actaattctt tcactttatt tgctccttta tcatttatta ataccgaaat aaccgctttt
    12841 gtgcaaaaac ctactatgtt tctgttaaag aaaaacaccc tccactttat cataagaaaa
    12901 gaagggtgaa atagccaatc ttttacttat agtccagcat ttacggtact ggctagaaac
    12961 tgtcatccat attatgacga tatataagta ggtatgtaaa actaataata gtgtaaattc
    13021 attttcttgc aactgttttg cacgtgaagg cagtttgcca tatttgaata aaaaatgttg
    13081 tttcaaatat tgaaaacagt ctaacactac tttctgaaac tgacaacgtg gtttacttat
    13141 ttttttgtaa aatctaatga tttttaatgc attattcctt tttgttcggg tttaaatagt
    13201 tatttttatt ttctcctatc taaaaaagca gcccacataa aaagaaggca tgacaaaaag
    13261 catgtcatag cccctttttc cgaataaata gtgttcaaaa gacagcctat cggcaataag
    13321 gcaagatttt ataaactacg ggaagtaatt tccgtaaatc tattcttatt cttttaagct
    13381 gacaagctcg tcaatcacta ctgcttttcc tgaagcaata gaatgatttg ctgcgattcc
    13441 tgtcaaaatc gacaaaatac catctacgtg agttgctgcc cgattccacg gatcttcttc
    13501 tttttcttca ccaaatagat catctaataa acgcggatct cctccgccat gtccgcccgc
    13561 ttcttgttcg acgattacct catatggctc accgaacatc ggatagacat ggatggactg
    13621 aagaacggct gctccttcat cctttttatc tccacctgca ttgatgtaag acttttcgat
    13681 cactttcatt tcgatccgtc ctttgctgcc attgaaagaa acattgaatc cttcccacgg
    13741 catatacgcg tttagcgaat acgtcaaaat cgcaccagtt tcatatttga ccgttacgcc
    13801 tagtgtatct tcaatagaga tatcatcgga aaatacactg cggtcacgaa tatatccact
    13861 gtctttttct gcttctaaat acatttctcg caattcctca ttggctgaca ggtctatagc
    13921 aaatggatca gctttagcta gttcgcttcc atagcatcga ctgtaaaata tttctactcc
    13981 gcgtttctcc gcattatgca tcccataaaa gtttaatcca ccgaatgcaa ataccttttg
    14041 gggacggctg ttcaaccaaa aattcactag atcaaaatga tgggtagatt tatgaacaag
    14101 aagcccgccg ctattgtgct tatttcgatg ccatcttctg aaataatctg ccccgtgttg
    14161 cgtatttaac agccattcga agtggataga aaacacatca ccaatcacat cactttctat
    14221 cagttccttt atcttcgtgt gatacggtgc ataacgataa ttaaaagtga ctcgcaggct
    14281 tcttcccgtc tgtttttgga ccgttattat ttcttgagct ttttcctcat cgatagtcag
    14341 aggtttttcg caaataacat catatccttt tttcattgca cgaatgatat aatcatgatg
    14401 cgttcgatct actgacgtaa taatgacttc agccggccgt acgtcatcca gtagttgctc
    14461 aaattgtgat ggcatacatg tctttacttt tttctgtcca tacttgttca caagtatccg
    14521 atttgcataa tccatccttg tttgagatac atcacaaaaa ccgactagtt cacaagtttg
    14581 atgatacgta gaggcaattg cctcataaaa aaaacgcgct ctcccaccaa gtccgacttg
    14641 tacatatttt ttcttcattt aaagtcattc tccttttctt ttaagcaaaa tactgtgaca
    14701 aatggcagcc ctggatacag taatgcagtg agtaaaaaat catgctgtat acaccagaac
    14761 agtccataca aaaggcataa ataccacaga aaccccttga cgttttttga caacagccag
    14821 atattccttt tgatcaaata aacaagacta tctgctttat cttctttgca gatatcaatt
    14881 agcgtactaa aaaaactatg ggatacaaaa agcaacagac acaatagaaa aggaatcaga
    14941 taccaagctc caatctttcc cactaataaa taccaatcaa ctgcaaaaat aatgacaaac
    15001 agctgaaaca acgtaatgat gaaacacatc cgtttgattt ttcctttcct ccaacatacc
    15061 tttttttctg ccaagtaatg gacagataga taataagaag gatacatcat caaaaaataa
    15121 atcagataga caacacgagc agacagcact aatcctaaag tgaacaaaca tcctagaact
    15181 aaagcgaaaa gagcattact gataagactg gttaaaaaaa tccgacagac atagtagttg
    15241 agcttgtaaa gaaacatctc ttctattggc tttttcatat tcagcccttc agaccgcttg
    15301 tactgatccc ctggacaaga tatttattga agaataagaa aatcagaaaa accggtagca
    15361 gcgacaacgt agacatcgca aacatactcg cgtaagtcgt actagatcca gcgtctgcaa
    15421 ataatttgat ggcaatcgat accgtgtaag atctcggctt gtttaaataa atcaatggtc
    15481 ccatgaagtc gtcccatttc caataaaact ggatgatgat cgtcgtgatg atcgaaggtt
    15541 tcagcaaagg aaaaataatt ttggtaaaga cactatactt gctgcatccg tcaataatcg
    15601 ctgattcatc taactctttg gggatgctga ccatgaattg catgatctgg taaatgaaaa
    15661 atgcttgtcc aaaaaaatgt ggtaaaatca gcggcacata cgtcccaacc aaattcagcc
    15721 gattgtaaat aataaactgc ggaatcaaaa tcacttgtgc aggaatcatc atcgttacta
    15781 acatgacgac gaaccagaat ctttttcctt tgaaatcgat tctcgataat gcgtaagcaa
    15841 tacaagtcga gctcagaact gtccctatcg tcgaaactgc agagatgaca aacgaattcc
    15901 taaagaaagt aaaaaatgaa attccagcaa accccgccca tccttttgcg taattttccc
    15961 attgaaaagt ctctggaatc aaattcagcg aaccactcat aatctcttga tccgttttaa
    16021 aagaacctgc gatcatccat aagaccggat aaatcatgac tagacctaat agcaaaataa
    16081 aaccgtattg aaaccatttc gttactgtat tattcatttc ttcctcctct cattcacaca
    16141 acgacccatt tttttgatga tttgaaaatc agaaaggtga cagctcccat aatcaacagc
    16201 atgatccacg aaatagcact agcatacccc atagaattaa aagaaaaagc ttggttatac
    16261 acataaagag cataaaagtt cgtggcatca ttcggaccac cttgggtaat gataaatgcc
    16321 tgcgtaaaag tcataaacgc cgcgattgtc tgcataacca agttatacaa aatgatcggc
    16381 gaaagaagag gtaacgtgat cttgtaaaac gtctgccatt tgttcgctcc atcgatctct
    16441 gccgcttcat aatacgagcc tgggatctct ttgatacctg cagcaaagat gatcattgcc
    16501 gaaccgaatt gccaagcaga catcaaaaca agagggatca tgaccagttt ttgatcgcta
    16561 aaccaggaaa gcgcaggtgc gcctaatcgt tccaggaaaa tattcactaa cccgttttta
    16621 gaaaacaact cccgccaaac tagagcgata gaaatactgc caccgatcaa actaggcaca
    16681 taatacaaag aacgaaagaa agtcgttcct ttgatattcc tcgttaaaag ataagcaatc
    16741 aatagaccaa agatcagttt cacaggtaca gatgcaaata catagagaaa agtgactcgg
    16801 atggagttca aaaaacgggc atcactcagc atctttatat aattatccaa gccgatccat
    16861 gagactggat ttcccaaacg ataatcggtg aacgaataat aaagtgacaa gatcatcggt
    16921 atcactgtta tcaatagaaa tcctaggatg aatggagaag caaatacgta accgaccaca
    16981 ttgtcttgat ccaagaaccg ttttagtttt tcttttttca tgccttcctc ctgctgctat
    17041 cctagaatgt ttgacgcaaa ctcataaaga ctctctgctg cttcttctgc tgttacttct
    17101 tcataaatga ctttttcaac taaaagattg tattggtctt gaatttccgg attcaaagca
    17161 ctatcaaaga cattcacttc gccgttgtcc atatcggaaa tcatttctaa ataatcaaat
    17221 gtcgcttgca aagttgcatc atctgcacga gcgagctgtt ctcgaatatt cgacatgatt
    17281 ggtacaccac gttctccttt taaaatttca ttggcttctt cactgttttg aaagaaatca
    17341 ataaacttag cagcttcttc tttatgggct gagctttttg ccatactcag catttgcgaa
    17401 gaacgtaaag ggataccaga ttctccatct gcttttctgc gaggtggatt aatcaactga
    17461 atctccatat cttcaggagc ccctgacaat atttctgtca actggttact tgatagaaac
    17521 accattgccg cttctctatc cctaaccgcc tgaaaagaca gatccgtcgt acttgcagct
    17581 ccaccacctg gttctggatg ggcccctttg tctaccagat ttttacgcag ctggaaaaaa
    17641 tctgttagat agtctggatt ttcaaatccc aagctgttag atagatcttc ccgattataa
    17701 aaattcaatc ctttctcaac ttgtggtatt tgaataatac agccagctat aaaatcttcg
    17761 aatcttgaaa tagcatagat tcctaattct tcagtgattt gatagctgat ttgttcaaat
    17821 tcatcccatg tccaatttga agctggttca gggatattta attgttcaaa tatattttta
    17881 ttgtaagcaa tcgcaaacga attcaccccg ttagacagtc caactaattg cccgtcgtaa
    17941 gtcgtcgttg ctaaatagga ttcatctgca ttagaaatat caattatccc ttgctccaca
    18001 tattcatcta atggttcaat ctgattgatg tattgaggaa attgattgtt cagttggaaa
    18061 atgtcccaga tatcatctga tgctgctagt gtacctaatt ttgtgtaata tccttcataa
    18121 tcataaaatt cataatcgat cgtcacatgg ggattttttt cttcataaag ctcgattact
    18181 tttactgttg catcgtgtcg gacttgcgaa ccccaccaag ccatgttcag cgtgattttt
    18241 ttgtcatctt cttgcccaca agccgtcaag aagaataaca taacacttaa agctaagatg
    18301 attctcattc ttttcttcat tctctgcacc tctacttcca ttgttatttt tcaatatact
    18361 gtatttttat acagaacata tttattaaac gatctttttc aaggggaata aaaaccaaga
    18421 cttgccttat cgttctgcct aggatgaaca actcaatact ttcaagaaaa attagatagt
    18481 gctgattctt ctttcatcgc taaaaacact tcttgcttca atgatgggag gataattacg
    18541 caaagaaaaa aggatatcag acaactgccc aatatccttg acatagctaa ataagcatgc
    18601 ttcttcgtgt gcgctcttcc ctaaaaaaca gaaaaataac tagtaattct gtatgaaatt
    18661 aggtacaata tacacgtcga tgcacaagca ttgactatgg caacttgatt ggtcagttcc
    18721 cgctgacttt tcaagccccg acaatttagc tgctactaaa ttgtcgggtg ccttttttag
    18781 tttttattcg tttttcattc tatagaaagc gcttcaataa atcaagacat ttttttgtta
    18841 gaatgctaat aataacaaaa ggttttaact cttcttatgt acattacagg taaaaaaaca
    18901 aaaaaatttc tctttttttt gaattctgaa caaatagtac tggagtacaa aagacgagga
    18961 agagccacta tctgctcttc ctcgtctttt gattctcacc tgttattcat gattcttttt
    19021 ctcagtttcc cgatattttt ccattttacg actaaaaagc tctccgctac ttggtggcgt
    19081 atatgtgatg gcatttgctc ctgcttgaat ggtctcagca atactttctt ccgttggacc
    19141 acctgtcgca atgataggaa gttcaggata ttttttacga aaatggcgta ctgcttctgc
    19201 agtttcttta cctgcactga tattgataat cgaaatacca gctgctaact tttcatctat
    19261 ttctgtatat ttcgacgtta ccgtactgat aactggaata tcaactactc gacaaacttc
    19321 ttttactgtc tctaaaggcg taggaccgtt caaaacgacc cctaacgaac cttgcgcttc
    19381 tgcgaacatg ctcatgtagg aagaacgagc accttgtgtc aaaccaccac ctacaccgga
    19441 gaaaactgga atgtcagctg cctcaataat gcttttagta atcgctggat gaggtgtaaa
    19501 aggatacaca gcaattactg cattcgcatt tgtattacgt atgatcgcaa tatccgtagt
    19561 aaagatgatc gaacggatca attttccaaa tatcttgatc ccgcttgctt ctaaaatgat
    19621 ttccggtact cttacgatat cttttctcaa ttccgtcatg atttctggcg taaattgttc
    19681 tgccatcagc cacttctcct ttgctatctc cggtcatact gattcaattg atacgtctcg
    19741 atgacctgga tgattttttg cgcataaccg gggtctgtcg cataacctgc atcttgcaat
    19801 gcttgtgcgg cttcctgata gttggtggcc gtaatcactt tttcatacag cgcaggattc
    19861 caatctactc cttggacgaa cagttgtgta tggtcatcca tggactgttc ccatgaatca
    19921 tacactttga agtctccttg gatagttatc cattcttcgt taacaaattc ctttgtttcc
    19981 aggcttactt tcttctgatc cccataagcc ttgatcccga aaagattatt gtatttgcta
    20041 gccaaggtac tttttcccca atttgattct aatatagctt gtcctaaaat gatactagga
    20101 agcactccat aaccatcttg tagttcttgg gcatgcggaa caagccgttc aataaattgc
    20161 tgatgggatt gcgcttcatt ttcttgctga ttatcagtaa aaggagaaga cagattcttc
    20221 aaggaaaaaa caaaggctaa gagtatcact gctacaccgg caaccactgc tggccaacgg
    20281 atattcttgc gcttttgctt tttccacttt tttttcgcca tgatcaattc ctacttttca
    20341 tttaattcat tcagatattc ttctagcgtc aattggtgtt tggtgatata ctcagcgttt
    20401 tctttaccga cataacgtaa gtgccaaggt tcatatgtga tcttagttat atcttcgtgt
    20461 ccttttggat aacgaacgat aaatccatac tgcgcagcat tatctgcgat ccatttagct
    20521 ccaggctgat ctccgtaact tgcatccaat acagtagaag gataactgtt gtaccagttt
    20581 tgatcgacga catcgactgc cagacctgtg tggtgttcgc tgtatcctgg ctccgtaatc
    20641 gtttctttag taatctttgt cgcctcctct tctgatacac cttgacgact catcacatct
    20701 tggacatttt gttcgaaaac ttgttgttgg gatgatacag accggtaagc tgagaccatc
    20761 actaatggaa atccagctgc ttcagcagcc ttggcaaatg cttcatattc tggagcaatg
    20821 cgtttgtcga tcaagtatcc gttacttaat gatgccaatt gagtagactc atcaatttct
    20881 ttttctaatt tgtgatctgg accaaccaaa actagcttcc aatcgtctgt tttaacatct
    20941 ggcaattgtt tggacgtacg atcttttgtt tctttgtcct ttaccttgct gctggttgtt
    21001 tgactgttct tttctttact agcagttgtt ttggtgcttg ttcgttcgac tgcttgattg
    21061 ccggcttgaa catgcgaatc atcatcgcta aaaataacat agcccattgc tgcgatcata
    21121 atcacaactg ctccaaatcc taaaaattta ttcacttgaa aaatcctttc gttgttcttc
    21181 acgttcccca ttcgcctcat gaacgtaagt ttcttccaga ttttcattca cccaatcgag
    21241 aagttcgatt ttttctcctt cgatctgttt ttgaacatct gctggcagac gaaaattcaa
    21301 aatatcatca tagccccatt catcatattg aatacttact ttcaagtcaa ggtatctacc
    21361 agcctgctga ttatttttca aagggatcag ctcgactttt gctgctttgc tgctaagcca
    21421 tttttgcgcg atgcgtgttt tgatttgctt gtactctgca acggcaacgt ttcgtttttc
    21481 gggaaaaata atcgtttgac gcaaaatctt ttgcgttagc atccattcat aatcagtgaa
    21541 aagatttttt actttctgca tcgcttcaga tggcagattt ttctcgccat ctttccattt
    21601 ttgccattgt tctggactga tcagcaagta ttcatctaaa aaattttttt cagtttcaaa
    21661 ctgttctaat aatacactca taataagatc aatatatact tgatgcacgt ttatcccctc
    21721 cttttaacta ttctacttaa gttttgatca aaaagcacct cttaaatcgc tgctttcatt
    21781 ttttttaatg tttcaagtcc tctcctcttt attcttcgtg atttttgtta taataataga
    21841 cagacaagaa actcaatcca actatgaagg agcgttttca gatgaaaaaa tatcaatggg
    21901 gaatcgttgg tcttggtacc atcgcacatg agtttgccga aagcttcaat caagagacga
    21961 gcgaactaac agcagttgct tccagaactt cagaaaaagc ggaaaacttc gctcaccgtt
    22021 acaatattcc aaaagcttac ggaagctatc aagaaatgtt agacgatgct gaaatcgaca
    22081 tcgtgtacat tgctgtacca aatagacagc atatcgacca tatcttggca gcattggaag
    22141 caggcaaaca cgttctatgt gaaaaagcca tcacgatgaa taaaaaagaa ctggctgacg
    22201 ctatgcgctt agcagaagaa aagaatttga tcttagcaga agcgatgaca atcttcaaca
    22261 tgccacttta tcagcagtta cgctcaataa tggatacagg aaaactgggg gctttaaaaa
    22321 tgatccaagc acctttcggc agttacaaag aaccagaccc taaaaaccgg ttcttcaatc
    22381 cagaattagc tggaggagct ctgttggata tcggtacgta tgctgtatct ttcgctcgtt
    22441 tctttttgag ctctcaacct gaagtagttg cctctacaat ggttcctttt gaaacaggcg
    22501 tggacgaaca atccgtcact atcttgcgta acaaagaaaa tgaacttgct gctgtcagct
    22561 tgacttttca ggcaaaaatg ccaaaagtcg gtatcgttgc ctttgagaac ggttatgtga
    22621 cgattgctga ttacccgcgt gctgatcgtg cggagatctt atttacagat ggtacaaagg
    22681 aattcatcga aagcgggaat acatctgaag caatgaatta cgaaataaag aatatggtac
    22741 acatgattga aggtgacttg cccaatcgct ctctcttctt gacaaaagat gtcatcgaaa
    22801 tcttggatca aatgcaagtt ctatggaaaa atatgtaatt ttaaaaggtt gtgaaaaagc
    22861 tgttctgcat caagtaataa gaacagccaa acaaaaatag ctcctcctat ttttcgtttg
    22921 gctggactta ttaccgcaga ggaaagcttt tgaacaccgt ttaagaaaga ggttgtgagc
    22981 ctaccgttca gctccgagtc acaaggagca atttcaaaaa acagcttctc atgtttgttg
    23041 aatattgtga cttgtgaccg aggagttgac aggagaacac cgtttaaaga agaggttgtg
    23101 aaatcagctt tttgacctga atattagctg aattcacaac ctctattttt ggattttttg
    23161 ttgggagaat tctgccttga tttcaataaa acttttaaaa gactgcaaaa actggaacaa
    23221 ttctgctcga tcctcgaatt cagggcgact agtcggcaga ggcatttgcc gatacaatac
    23281 ataaacttct tcaatacgtg ttaaaatttg tctgccatca tttgactctg aaaatgtttc
    23341 tgcagtataa atcaataaac catagatatg ttttccataa taagctggtt gctggattct
    23401 ctctagattt tccagcatgt ctttaatcac attggcctgt gctcttctca tagagaagta
    23461 agtttcgtag tatagcggct gtctgagcca gtagttttct tgatgttctc tggcatcttc
    23521 ttggctttcg cgaatgaatg tcagtaaatg ttcgcatttg atctgcacat cttctttatt
    23581 ttctgataaa agaaattctg ccatttgtct caaaatattt cgaaattctt tttcgatctg
    23641 tatttggttt tccttcagct gcttcgtacg atctggcatg taaacatttg caagcaaagc
    23701 aaatactaat ccaatcgcca ttaatcccgc ttcattgccg atcagctgcc aagacatgtt
    23761 ttcctccact aaataatgag tgaccaaaac cgaatttact actatccctt cggttaattt
    23821 gaagcgagag gcaagcggga tgaacaggaa taaaaaaaga ctgaagccaa aaatagtata
    23881 cccaacagca aaaaaactga acagtgctgt tgccgtagct aatataaagg ctgcgattcg
    23941 attcatgccc gtacgcaatg tagagcgctt ggtatttcct acacttaata atgcaatgat
    24001 ccctgcagct ggccaataca acagcgataa gctacccgct gtgaccatcg aaaagaagac
    24061 actgatcact gttttgactg ttcgcattcc tatacgcatt tacttccccc ttttttattt
    24121 taattatact tgaataatga aaaactagct atcagacaaa agtttaggta tgatagtaaa
    24181 aagaccaaaa aggagaatct ctatgcaatt tggccgtttt cgactaggta tgcgtacgat
    24241 caaaagtgcc ttagctgttt ttttatgtat cttgtttttt catttgactc aacgaggaat
    24301 cccgatgatt gctgcattat ctgctgtttt ttctttgcgt caggacttga ctacaaccgt
    24361 ttcttttgga aagtctagga tcatcggcaa tacattaggt ggtacattag ctatcattta
    24421 tttttttgtc aaaagctatt ttcacaatga ttttttggta gaattgatct tgcttcctat
    24481 tctagttatt attgtgatcg tcgtgtcaga tggtattaac aataattccg gtatcatttc
    24541 cgcaatagct acgttgttgc tgattgcgtt gagcatccct caaggcgaat ccgctttgta
    24601 tgctatccaa cgtgtactag atacttttat agggacattc attgcaatcg gtatcaattt
    24661 ctttcttcgt cctcctgaaa cagaaaagaa ggaagaacta tcagaagatt tggtcgaact
    24721 ccaaaagaaa gaacgattgc taagaacaaa tttacaagag atagaagcta agatccaaaa
    24781 acaaaagaaa aatagcgagg actaattttc ttttgcaata aaataaaaaa atggagagaa
    24841 gaactttaaa aagtcttcct ccatttttga ttgaacggtg aagtgtacta ctttattctt
    24901 ccttcatcga gttttgttct gcctcttttg gcgcttcata catatttact tggctagtag
    24961 tcccacgtgt cgacaaattc tctacgattt ccttcaagtc aagtcctgtt gtcgctttta
    25021 gtgtttcttg agtagaagaa agcaaatttg tggcataatt tgtcactcgg ttcgctccac
    25081 tggtttctcc tgaactgctt cctgtatcga caacagagat tttttcgata tttcctagcg
    25141 gctgtgctgc ttcgcgcatc aattgcggta gcatatcgat aaccatactc aagacagctg
    25201 cttcgccata ttccttgaag gcattggcga tcttttgttt tgcttcggct tctgcttttc
    25261 cttttgctag tgctgcttcg gcttctgctt gtccagctaa acgtgtctta ttggcttccg
    25321 cttctgccag tgcttccaca cggaatcgtt ctgcttcggc ttcagtcact tctttgactt
    25381 tttgagcttg tgcttcttgt tctttagcgt accgatcggc atctgctttt ttcttcactt
    25441 ccgaatcata ttgcttttca cgacgtgtga tttctttttc ttccagttcg atttgttttt
    25501 gacgttctac gattttaatc tgcatttcct gttcgattac ttgttgttgt gctcgggcac
    25561 tttctaaatt gtaggcttgg tctgcttttg ctttagcaac atcttgttct tgtttgtagg
    25621 ttgctaattt cagttctttt tcttttaatg attctgcgat ctctgtttgt cgctgtaatt
    25681 cagctgcttg tgattctttt tctgcttgag cttttttgat acgcgtttct tttagagctt
    25741 ccgcttctgc tatatctgca tctcttttga cttgggcgat tcttggtttc cctaaagagt
    25801 caagataacc gtttttgtct ctgacctctt tgatagtgaa agaaacgatg atcaacccca
    25861 tttttgcaag gtctacagaa gcgacttctt gtacgctttg actgaactta tcgcgatttt
    25921 gatagatttc ttctacagtc atagaaccaa gaatcgagcg caagtgtcct tctaatactt
    25981 ctcttgcttc attttccagt tcttctcttg tcttgcctaa aaactgttct gctgctgtag
    26041 caatctcttc tacagatgaa ccgattttga tgatcgaagt accatcacac atcaccggta
    26101 cgccttgttc cgtatacact tcaggggtgg agacgtccaa tttactcgat aatagactga
    26161 tccgattcga acgttgaaaa actggcaaga cgaatgcgcc acctccacgc acaattttga
    26221 ttttgttatt gctttcatca atgtgcacat ttttgcttcc taaataactt ccactgatga
    26281 tcaacgcttc atccggtttt gctgtctggt acttcgctac aaagacgatc agcaacatca
    26341 gtaaaatcaa agctgcgatt gcgatcgcga taaaaacaac tggtatttcc atttaacaca
    26401 cgctccttta agcttttatc atgctttcat aaggcatgac ataacagatt ctttccttga
    26461 tctcaatgat caatactttc gttccccgct cagctgtctg ttcttggggg ctgtaaaaac
    26521 aagctggacg actaatggaa ccggtaacac ttttcaactg aatttctccc atacctcgta
    26581 cagggatcgg tgtgatcaca acagctactt gtccttccag agttttttca gaagacgaca
    26641 tcgttgattc ggcatgtttc atcggcatca atatatagaa attaagcaaa aataccaaca
    26701 ctatcgatat gcctccacta gcaataaata aaaataaaga tgattgatct gttaaacgct
    26761 cacctagaaa accaaataag cttgtgaaag ttaaccaagg gataataagc atcggatcaa
    26821 gcgggccatc aaaatcgaat acatctccaa aaataatcaa taggactgcc aatcctgcac
    26881 aaatcagcaa tgtataccaa taaatcgtct caagtgcgat tccccctatc atatacacgc
    26941 ctccttccat ttctacgtct atcataccaa tgtttgaaaa ttctgtatag cttcttttac
    27001 cgactcagtg gtgtttttta taggaaggtt gtgtttatcg ttgacaattt gttaaatttt
    27061 tgttttcatg ttaaaatttt cacaataaat tctttcgagg tagcctatgt ttcctttttt
    27121 taagaaaaaa ccattaaagc tccagaacac gatcatcctt gtcgtttgtt tctccacatt
    27181 cgtttctttg attgtttctg ccatattgat caggaatttt attctttcga tgaaccgatc
    27241 tagtgtgtca gataaaatct ctagcattgc taaaatcgtt gccaatgata aactcatcca
    27301 aaaaaagctg cagactggtg acccgacaca tgcgattcaa ggaataacaa atgaaatcat
    27361 gattgcatcg aatttagatt ttatcgttgt catggaccct gaaggcattc gatattctca
    27421 cgcagaccca agttacatcg gtcttccttt ctcaagtcat gaagatgtga aaaaagtgat
    27481 tggaggacaa gaatttttct ctgaaaaaga aggaatgtta gggttcggct tccgctattt
    27541 cgtccctatt tattatgatc aagagattat tggtgcagtt tgtgttggct taacaaacaa
    27601 cacgattgac aatcaagtca aagatggaca aaaaatgatt gttttttcat tgattattgg
    27661 acttagcgta ggcatcatag gcgccgtttt tcttgcccaa aaaatgaaaa aagtactgct
    27721 tggacttgaa ccacaagaaa tagcagaaca gttaagtgaa aaaacgatta tcgaaaatga
    27781 agtagatgaa ggaatcttag ccatctcttc tgataaaact atcctcttga tcaatacacc
    27841 tgcaaaaaac attttctacc aaacagagca gctattggat ttcgaagtag gaaaaaaatc
    27901 agtgataatc tatatgacgt tttgtttaaa gagacgattg agcatcaaac gaccgttcgg
    27961 actcaatcca tgtatttaaa tgggatagaa actatttcta gcgttacacc tctttttagc
    28021 aatgaccgtt ttttaggagc agtcgcaact ttacgaaatc gatcagaagt aatgaaatta
    28081 atggaggagc ttggggggac gaaacaatat ataaactcca tgagagctca aacccatgaa
    28141 ttcatgaata aacttcatgt catttctggt ttgattgatt tgaagaagta tgaagaagtc
    28201 acccatttta tccagcagct aaatcaaaat tataaaaaag agattggcca agtctcaagt
    28261 ctgatcaaag tacctgcaat tgccggtttt ttattaggca aaataaacga agccaaagaa
    28321 caatctgttg aaatgctgtt agaaagcgat tcttttatcc ctaaaatgga gatggatgac
    28381 tccatccatt cacttttgca agtgttaggg aacttacttg ataatgcaaa agaagccgtg
    28441 ctacaaaaac atgaaagaaa cggacaggtc cggattcatt taaactatga cacagaagct
    28501 gaagtattca tcgtgaccgt ctgcgatgac ggtgtcggga tatcagaaga aatctcagaa
    28561 aaaatattta cgctaggttt ttcgacgaag ggaatcaatc gtgggtttgg attgaatctc
    28621 atccattcaa tcgtcaaacg ccacggtgga tttttagaat tttccaaaca aacaaatggc
    28681 ggtacaatcg tctatctaga attaccaaaa aaggaagatc aaaatgatga caaatgtcct
    28741 gataatcgaa gatgatccaa tggttgcaga attgaatcgt acttttgtgg aaagcatccc
    28801 taatatgaac gtaatcgaaa gtttcagaaa tgtagaagaa ggaaaaaaat atttcagaca
    28861 aaaaaacgca gatctgctgc tgcttgatgt atacatgcct ggcgaaagtg gtatccattt
    28921 tttatcttgg cttagacagc aaggaaatga tatcccagtg attttgatca ctgctgcaga
    28981 tgatatcgaa accataaaga atgtgatccg ctacggtgcc ttagattatt tgatcaaacc
    29041 gttcactttg caacgttttc agctggcgat cgaaaagtat cttgctttct ctcagacaat
    29101 ccaaacgact gaaaaagccg atcagaaatt gatcgatcaa gtattcaacc acaccactcc
    29161 cattcacgaa ataccatcat tcatcgattt gcctaaaggt ctgtcaaaac tgactttaaa
    29221 aaaaataagt gcatttattc taaaagaaga tactattttt tcaacaaaag aactggctca
    29281 aaaaagctca ctgtcacgta tatccgtcaa gaaatatttg aattttttag tagaacaaga
    29341 tatattgaac gaaagaatca aatatttgga aatcggccgg ccgctaacct gttatgaatt
    29401 gacaaagaat gggcggaaaa agctcacaga ttactgttga gtcatcgaat tatcccatac
    29461 aaaatagtcc aagtatacag aagtaacaac tgtttacttg gactattttt aaacatttta
    29521 ttgacgaata tccatgtttt cttatcttaa ttgacgggct ttaggaagag atacctcatt
    29581 tttcgttttt ccagtttcta ttatttcacg aaagttctcg aaacttgtcg aaatcatatt
    29641 tgataatgcc tcatctgtat ttgaacctac atgcggggtt acaagaactc tagggtacat
    29701 ttctactaat tgctggatag cagggtctgg aatcgtctcc catggcttga aggatcggaa
    29761 aaaaacctct gtttcatttg caaaaacatc ggttgcaaaa ccagctaaat gattgctttt
    29821 caaagctctt aaaatagctt cattatcttg aagctctcca cgggctgtat taatcaagat
    29881 gctacctttt ttcatttgag ctaaaaatcc ttcattgatc atttgatcgt tttcacctgg
    29941 gatatgaggt acatgaatac tgacaatatc gcttttggct aataactctt caagcgagtg
    30001 gaacgtcaat acttttttag cagcttctgt ctgatgaata tcgtagccga tcactttggc
    30061 tcctagtccc ttaaacaaaa cagcttccgt caaccctatc ctgcctgttc ctatgatccc
    30121 tactgtgcaa tttctgattt cacgactaaa catttgctca tccactcgaa aatctttgaa
    30181 agatgttcgg atcgtcgtat acgcagtatt tctcaaaagc atcatggcaa aagtcaaaga
    30241 taactctgct atcgcatttg gcgaatacga tggtacacgg gctactgtca taccaaaatc
    30301 agctgccgct tgtagatcga tatgattgac tcccacagtc cgtgtaaaaa cataacgaat
    30361 cccatattcc tgcatttttg caagattggt ctcatctgct atacaatttc ccctcaacaa
    30421 tacagcatca tggtcctttg ctgacacgat attgtcatgt gtcaacagtt cttcataaag
    30481 actgagatca taatggtaaa tattcaattt ttcaaaaaaa tctatctcgt tcggacgaac
    30541 accgtaacat gcgattctca tactttcttc ctccatttct tatgaaaata aacctatgcc
    30601 atgaatcatt tgtaatacaa cgatccatac aggtgtcagt accacagcaa aaatagttga
    30661 cagtaaggat gcattggagg ctaaaagtgc ctccctatca aaactgatag cataggctgc
    30721 tgccactgtt gcagctggtg tagccatcat gataacgatt gttgccagtg cttcaaaatt
    30781 gactggtaga atattcgttg ccgtcaaaac tgctagaatc acgatattca atgcaggtac
    30841 aaataccact ttgactagac tgtaatacca tgaagttttt tcagatgcag cttttttgaa
    30901 gcttacttca cctaatgttg cgccgattgc tagccacgcc aatggtgaac ttaaaccagc
    30961 taaataatcc atcgactgaa atagccacgg tgcagtctgg tcaattcgca aaaacgctac
    31021 ttggcttatc tctccttctg cacctgtaac tgacacttgt ggcagatacc cttggaacag
    31081 ccaaatgaaa aggccggcaa aagtagcgat aatgattgga ttgacgaaca tcgctttgat
    31141 atttttagct tccatcttca agccactcat ttttatgtat ccataagaat acaaaaagat
    31201 tcggtagcca atattgaaaa ttgaggaata catcacgccg acaggaccat aaatcgcact
    31261 gacgataggc gttccgaaaa aggtcgtaga accaaagatc gtcaggacac gcaacgttgt
    31321 ttgcttatcc cctttatagc ttaagaaaaa aggttttgaa actagaataa gaataatata
    31381 aataactaat ccccaaatca agacattgat tccttgcatc agtaattctg tattgatatc
    31441 ttgcatgaaa gctcgaaatg ccagtgctgg caaagccact gttaagacga ctttcgatag
    31501 aatttttcca acagattcag aaaaaatccc cttttttcta caaacaaacc ctaataatat
    31561 aataaaaact gttgaagtga ttgcactgac aatcgacata tctgtcaaag tcgatcgaaa
    31621 aatgtcaaat aagttcatct ttctcctcct aattatgttc atttattcac aataaaaaaa
    31681 gcttttattt
//
LOCUS       DAZKMB010000018        31296 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000018 DAZKMB010000000
VERSION     DAZKMB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.018"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..482
                     /locus_tag="ACG8V3_000774"
     CDS             165..482
                     /locus_tag="ACG8V3_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0299337.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(686..1051)
                     /locus_tag="ACG8V3_000775"
     CDS             complement(686..1051)
                     /locus_tag="ACG8V3_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299338.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1259..1741
                     /locus_tag="ACG8V3_000776"
     CDS             1259..1741
                     /locus_tag="ACG8V3_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299339.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1938..2828
                     /locus_tag="ACG8V3_000777"
     CDS             1938..2828
                     /locus_tag="ACG8V3_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0299340.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3153..5624
                     /locus_tag="ACG8V3_000778"
     CDS             3153..5624
                     /locus_tag="ACG8V3_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0299341.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5727..7463)
                     /locus_tag="ACG8V3_000779"
     CDS             complement(5727..7463)
                     /locus_tag="ACG8V3_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0299342.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7460..8164)
                     /locus_tag="ACG8V3_000780"
     CDS             complement(7460..8164)
                     /locus_tag="ACG8V3_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0299343.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8295..8798
                     /locus_tag="ACG8V3_000781"
     CDS             8295..8798
                     /locus_tag="ACG8V3_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0299344.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8758..9096
                     /locus_tag="ACG8V3_000782"
     CDS             8758..9096
                     /locus_tag="ACG8V3_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0299345.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9117..10535
                     /gene="ffh"
                     /locus_tag="ACG8V3_000783"
     CDS             9117..10535
                     /gene="ffh"
                     /locus_tag="ACG8V3_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0299346.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10647..11225
                     /locus_tag="ACG8V3_000784"
     CDS             10647..11225
                     /locus_tag="ACG8V3_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0299347.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11229..11750
                     /locus_tag="ACG8V3_000785"
     CDS             11229..11750
                     /locus_tag="ACG8V3_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0299348.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11829..12383)
                     /locus_tag="ACG8V3_000786"
     CDS             complement(11829..12383)
                     /locus_tag="ACG8V3_000786"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0299349.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12636..12911
                     /gene="rpsP"
                     /locus_tag="ACG8V3_000787"
     CDS             12636..12911
                     /gene="rpsP"
                     /locus_tag="ACG8V3_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0299350.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12923..13174
                     /locus_tag="ACG8V3_000788"
     CDS             12923..13174
                     /locus_tag="ACG8V3_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0299351.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13299..14090
                     /locus_tag="ACG8V3_000789"
     CDS             13299..14090
                     /locus_tag="ACG8V3_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299352.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14260..14781
                     /gene="rimM"
                     /locus_tag="ACG8V3_000790"
     CDS             14260..14781
                     /gene="rimM"
                     /locus_tag="ACG8V3_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0299353.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14771..15532
                     /gene="trmD"
                     /locus_tag="ACG8V3_000791"
     CDS             14771..15532
                     /gene="trmD"
                     /locus_tag="ACG8V3_000791"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0299354.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15668..16015
                     /gene="rplS"
                     /locus_tag="ACG8V3_000792"
     CDS             15668..16015
                     /gene="rplS"
                     /locus_tag="ACG8V3_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0299355.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(16147..17283)
                     /locus_tag="ACG8V3_000793"
     CDS             complement(16147..17283)
                     /locus_tag="ACG8V3_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0299356.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(17399..17965)
                     /locus_tag="ACG8V3_000794"
     CDS             complement(17399..17965)
                     /locus_tag="ACG8V3_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFQ0299357.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(18101..18754)
                     /locus_tag="ACG8V3_000795"
     CDS             complement(18101..18754)
                     /locus_tag="ACG8V3_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299358.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(18804..19232)
                     /locus_tag="ACG8V3_000796"
     CDS             complement(18804..19232)
                     /locus_tag="ACG8V3_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0299359.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(19237..19611)
                     /locus_tag="ACG8V3_000797"
     CDS             complement(19237..19611)
                     /locus_tag="ACG8V3_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0299360.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            19915..20100
                     /locus_tag="ACG8V3_000798"
     CDS             19915..20100
                     /locus_tag="ACG8V3_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0299361.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            20113..20361
                     /locus_tag="ACG8V3_000799"
     CDS             20113..20361
                     /locus_tag="ACG8V3_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299362.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            20392..20562
                     /locus_tag="ACG8V3_000800"
     CDS             20392..20562
                     /locus_tag="ACG8V3_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299363.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(20664..21131)
                     /locus_tag="ACG8V3_000801"
     CDS             complement(20664..21131)
                     /locus_tag="ACG8V3_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299364.1"
                     /translation="MNYLIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFL
                     VKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLL
                     SPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            21290..21607
                     /locus_tag="ACG8V3_000802"
     CDS             21290..21607
                     /locus_tag="ACG8V3_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299365.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            21540..22019
                     /locus_tag="ACG8V3_000803"
     CDS             21540..22019
                     /locus_tag="ACG8V3_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFQ0299366.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            22036..22212
                     /locus_tag="ACG8V3_000804"
     CDS             22036..22212
                     /locus_tag="ACG8V3_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299367.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            22469..22900
                     /locus_tag="ACG8V3_000805"
     CDS             22469..22900
                     /locus_tag="ACG8V3_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299368.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            22906..23223
                     /locus_tag="ACG8V3_000806"
     CDS             22906..23223
                     /locus_tag="ACG8V3_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0299369.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            23342..24028
                     /locus_tag="ACG8V3_000807"
     CDS             23342..24028
                     /locus_tag="ACG8V3_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0299370.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            24003..25352
                     /locus_tag="ACG8V3_000808"
     CDS             24003..25352
                     /locus_tag="ACG8V3_000808"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0299371.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            25355..25816
                     /locus_tag="ACG8V3_000809"
     CDS             25355..25816
                     /locus_tag="ACG8V3_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0299372.1"
                     /translation="MGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIARNKNN
                     EVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNWDKRR
                     NKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            25818..26318
                     /locus_tag="ACG8V3_000810"
     CDS             25818..26318
                     /locus_tag="ACG8V3_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFQ0299373.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            26333..28645
                     /locus_tag="ACG8V3_000811"
     CDS             26333..28645
                     /locus_tag="ACG8V3_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFQ0299374.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            28892..29209
                     /locus_tag="ACG8V3_000812"
     CDS             28892..29209
                     /locus_tag="ACG8V3_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFQ0299375.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            29206..29367
                     /locus_tag="ACG8V3_000813"
     CDS             29206..29367
                     /locus_tag="ACG8V3_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0299376.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            29370..29669
                     /locus_tag="ACG8V3_000814"
     CDS             29370..29669
                     /locus_tag="ACG8V3_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299377.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            29669..29983
                     /locus_tag="ACG8V3_000815"
     CDS             29669..29983
                     /locus_tag="ACG8V3_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0299378.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            29976..30506
                     /locus_tag="ACG8V3_000816"
     CDS             29976..30506
                     /locus_tag="ACG8V3_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0299379.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            30503..30772
                     /locus_tag="ACG8V3_000817"
     CDS             30503..30772
                     /locus_tag="ACG8V3_000817"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299380.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            30702..30905
                     /locus_tag="ACG8V3_000818"
     CDS             30702..30905
                     /locus_tag="ACG8V3_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFQ0299381.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            30902..31189
                     /locus_tag="ACG8V3_000819"
     CDS             30902..31189
                     /locus_tag="ACG8V3_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299382.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
BASE COUNT        10541 a         5381 c         6414 g         8960 t
ORIGIN      
        1 gaaagtatac tttatatagt cataacgccg atggtaacta tgcactgtaa cgataatgct
       61 gctaaaatat ctttttacga aaaacatcac aaggaaaaac ttaactatgg tattatctaa
      121 acataatttt ttcggtattt aatatttaat taggagggaa tattttgctt ttttatagta
      181 attttatctt aatagtagcg atattattac ttctgaatat atggattttt gataggagta
      241 gaaatgcaag tataggtttt agaactaaac gaagtctgtc ttctaaaaaa aattgggtat
      301 actcacaaac tattttttat ggaggaattg ttttaattag tttactttct tcaacacttt
      361 attctttgaa tattatagat gtttccacct caaattcaat atctattatt ggaatcatta
      421 ttgctgcaat tattacacaa ctgtttttag tgtttggaga gaagaaaaga agtaaaaaat
      481 agaaaaacta gtgatagttt tggtgatagt gatattaaaa atcagaaaag atagaaaaag
      541 caggagatag ataaaatgct gatatttcag cattttttaa gagtataaaa gagaaaaaaa
      601 gtaataaatg tatacatgat ataagaaaaa catatatgta taaaaagaag aaagtagctt
      661 ctctttgtac atatatggtt tgtttttatg gatgccgagc ttttttataa gataaggcta
      721 ccgcccataa cgacccaagg atttgtggaa ctaagtaaat acctgcaaga agcaagatca
      781 gctgggtcgg ttttaaagga tgatagaagt aacgccattc agtaaaattg gcgcaaaata
      841 aataggaagt gacagtagat ataatattgg caatgaataa agatataata cgcctgttcc
      901 tttgaaaata atatccagca aaaagagata aaaatatcaa tgtgatgaac ccgcttacgg
      961 aatgataacg aaaatcaagc agtaaggcga aatgcacata aggaacaaaa gataaaagca
     1021 acggaaggtt tttccagcaa gtttttatca tctttcatca ccacctaaca agtgttatta
     1081 ttttgttagt tttattgtaa attgaatcct gcaataatca attacttttt cattatttct
     1141 agattaaatg aataaactct catgccatta tctctaaaaa aaaagttttt ccccctaatt
     1201 tttttagata gaaaattgta tagtatgatg agagaaaaag tagtttgggg gaacgttaat
     1261 gagaagaaaa gcagtaatcg atcggcttta tttatttttt tggttttttc ctatcgtttt
     1321 gctacctttt acatgggtaa atgctgttgt acaaggtgcg gagatattag gcctgatcca
     1381 aggaagcgga tttgtggttt ctaggaattt tccagctttg actgttttct atttagtgat
     1441 aagtgtaggt ctcttattac caaaatctct gatcaatcac tacaattggc agctgcttta
     1501 cttttcttgc ttattagtat ttacatctat gttttctttg ctgcctcgct tgattatcgg
     1561 ccctaatggt ttgagcgaga gtggattgaa ggattcagct tattcctcca tgtatttctt
     1621 gacgatcaaa ccatggttct atttagtaat tgcctcattt attcttgcaa cattttttta
     1681 cttctggaca gaaatcagga taaaaagtga acgatctgta tctataaaaa ataactattg
     1741 atggtggatc atcagtaaaa aagcatatga gctgcgtaaa atgcatgttt ctcatatgct
     1801 ttttttatta ttatgtaaag aatgcgaaaa acgtttacat gatttttaca taacttaact
     1861 atatttttac agcaaccata cgagggattt acagtcgact gataaacttt agttgtaaat
     1921 aagaaaggag caataaaatg aaaaaagtat tgtttactct aggtatgatg ggaattcttt
     1981 tagccggatg cggctcagga tcaggaaata cagatggctc tgccacacaa aataattcaa
     2041 gtgactcaaa tgaacaagta aaaatcgttg cggtaggttc gacagcatta cagccgttag
     2101 tcgatgcagc acaagaaagc tttgtacaag aaaatccaaa ttatcaaatc tctgtacaag
     2161 gaggaggaag cggaactggt ttgagccaag tcgaagctgg agcagttact atcggaaact
     2221 ctgatgtatt tgcagaagaa agagacggag ttgatgcttc aaaattagtg gatcataaag
     2281 tagcagtggt cgggatggcg ccaatcgtca ataaagatac agatgtcaaa gatatcacca
     2341 aacaggaatt gattgatatc tttacaggaa aaatcactaa ctggaaggaa gttggcggga
     2401 aagatcaaaa aatcaacgtc gtgaaccgtg caaacggcag cggtacccgc gcgacattcg
     2461 aaaaatgggg actggatggt gctacccctg tgcagtccca agaacaagat tcatcaggaa
     2521 cagttcggca gctggtaagt caaacaccag gagcaatcag ctatttagct ttctcatatc
     2581 tcgatgattc cacacaagca ctaagtatcg acggtgtaga accaaaagaa gagaatgtag
     2641 cagataacag ctggggaatc tggtcatatg aacatatgta cacgaacgga aaaccttccc
     2701 ctgaagtcca aaaattctta gactacatga tgacagaaga aatccaagaa ggacctgtca
     2761 aagaattggg ctatttacca atcacaatga tggaagtaga acgtgatcac gaaggaaata
     2821 tcaaataact agcagataaa catgccacgg tagtcgttac tattaaggaa aaatcaacta
     2881 tgtttcccac atatacattt ttcctttaat tgtgaaaaat agttcctcat attttgccgt
     2941 tttttgagct aataccgagg gtcagctgat taaacaccgt ttattcgaaa aaaagagcta
     3001 cgacatagaa ctgtcgtagc tctttttttg ctatatgaca ggaatgaaag gtgtttgcaa
     3061 gcttaatgtg aggaacactt tccatatctg ttctatactg gataggagaa gtttggaaat
     3121 atccagaaaa cgaaatcaat cgaggtagcc acatgaagaa taaatggata aatcgaagta
     3181 aaaaaaagaa agtaacacaa agatcatcag ataaagtcat ccgtcttttt ccatcgtata
     3241 aagaaggatt aacggcagat caagtcaagg aacgaataga aaaaggtgca gcaaataatt
     3301 cagtcgatcc cacattcaaa acaaatcagc aaatcgtatt ggaaaacata tttacgtatt
     3361 tcaatttgat ctttcttatc ttagccattt tgctttgttt agtagaatcc tataagaact
     3421 tgactttttt accagttatc atagccaata caggaatcgc catttttcag gaaattcgat
     3481 ccaaaaagat cttggacgaa ctaaatgttt taaatacagc aaaagtcaat gtcatacggg
     3541 acgggataga acaaacaata gatatagaag accttgtaat agatgacatt gttattttag
     3601 agactggaca tcaaattcca gcagatgcag aggtcgtgga aggaaaactg caagttaatg
     3661 aggctttatt gacaggcgaa gcagatgaaa taaccaaaga aataggcgat ccattaatgt
     3721 ccggcagttt cattgtttcc ggaaaagctt acgcccgatt agacaaagtt ggagcagatt
     3781 cgtatatttc ccagctgaca gtcaaagcca agacaatggg agaaggagaa cagtcggaga
     3841 tgattgcttc tcttaatcaa ttgatcaaat ggattgggat catcattatt cctatcggta
     3901 tcctgctttt ttctcaaagt tactttttta atggaaacac gatcaaagaa agtgtcgttg
     3961 caatggaagc agcattgatc ggtatgatac ctgaaggact gtacttgctg acgacgattg
     4021 cattagcatt aagtgcgact aaactagcaa agcaaagagt tcttttacac aatatgaaaa
     4081 gcattgagac attggcaagg gtaaatgtcc tttgtgtaga taaaacagga acgatcacag
     4141 aaaataaaat gtctgttcaa aaagtgatcg tctctaaaaa acaagaaata atcagtcagt
     4201 cagatcaatt ggaagcatgc attgctgact atgcaaaagc gatggcagga gacaatgcaa
     4261 caatggaagc tgttaaagca tattttacaa aaaccactga tcaatcctat caccatgtta
     4321 ttcctttttc ttctgtccaa aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt
     4381 taggagcgcc cgaaatggtt ttgcgcgatc aattgccgga atatgcggaa gagtttgttt
     4441 ctttcgctga aaaaggctat cgcgtgttgg tctttggtgt gtacgatggg attcttgaag
     4501 aaccagtatt gacagaagca gtcacaccat taggctatat tctgattgct aatcctatca
     4561 gaaaagaagc aagagccaca tttgattact tcaaaaaaca gcaagtagcc atcaaagtga
     4621 tttctggaga taatccgctc accgtatcca atgtagctgt ccaagcaggg atttcacatg
     4681 ctgatcagta cgttgatgca tctaaattat cagaagaaga atatgaagca gctgtcgaaa
     4741 aatatacagt ttttggacgc gtcaagccag aacaaaagaa aatcttcgtt caattgataa
     4801 agcgtaacaa tacagtagct atgacaggtg atggcgtgaa tgatattcta gccatgaaag
     4861 aagcagactg cagtatagcg atggcttccg gaaatgaagc cgccatgcag gcctcacaag
     4921 tggtcttact tgattctgac ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg
     4981 ttgtgaacaa tattgagcga tcagccagct tgtttttagt gaagaatatt ttctcctttt
     5041 tgctctcact gttttcagtg atctttgctc tgacctatcc tttagaacca tcacaaatca
     5101 cattgattag tttgtttact atcggacttc catctttctt gcttgctcta gaagaaaaca
     5161 aaaagcgaat cagagggaaa ttcatcatga atgtgatgga aaaagctgtg ccaggtggac
     5221 taacagatat gatcgtagtc ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata
     5281 aaacagatgt ttctacagca tctaccatgc ttttgattgc agtagggttc ctcgttctct
     5341 ataaaatctg ttcaccgctg aataaattca gaagtcagat cattcttttc tgtgctagcg
     5401 gtaacttttt ttcggtcatc tttttgcata agctgttttc aatcaccggt atctcagctg
     5461 tatcactttt gctgttgagc attctatttt tctcggcaga ttcgattttt aggcatttga
     5521 ccacattggt agaatatttg tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc
     5581 gcgtagctaa aaaatttcta tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa
     5641 aaagagtaaa aaagaacaac cactcgtgta tctttcgaag caggatgagt catccaactt
     5701 taaaacgcac gagcagttgc tcttttttac attttcggaa tggtaacggt gaaagtcgaa
     5761 cctgttccaa gatgactgtc tacagtaatc gtaccattaa ggagttgcac gtagtctttt
     5821 acaatcgcta accctaatcc ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg
     5881 taaaaacgct caaagatccg ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg
     5941 acagaaaacg atagatcatc tgttgcctga tagcggataa taatctcgct gtcgcttttt
     6001 gaatattgga cagcattttc aatcagattt ttgataattg gataaaggat atctgtttta
     6061 gtagtaaatg gttcgttctt tccaatcaat cgaatcgtca accgtttctt ttcgatgatt
     6121 ggctgataat tttgaacgat ttgctggaaa taaggttctg tataaattgt ctggatctcg
     6181 taagaattct cgccatcttt tgacagctgg atgatttccc gaatcagttt atctaaccgg
     6241 atcgcatctt tttgcatgat ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca
     6301 ccatccagta aagtctcggt aaaaccaatc agtgaagtca caggagtttt taattcgtga
     6361 gagacgttgc caacgaaatc cttttgtagc ttttctaaac gtcgcacttt cgttaagtca
     6421 tacgccaccc ctagaatctg tccggtacct tctgaattag taaagtagcg caaactcaag
     6481 tctaatgtct gccgaccttc tgtaagggtg atctcctgat ggatcagcgg atgctcagga
     6541 gtcacttgat gaatcagctg aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt
     6601 tcaggttgat agcttaccac gtttaaatgt agctgcatct ttggattaag cagtagtaaa
     6661 cgagaatcag catcatcaat caggaatacg ccaatcatca gttcgttcag taacgtatat
     6721 aattgctctt ctgtggtagt acacgcccga tacatcttac tcatttgttc gctcaacgca
     6781 ttaatcgtct gatacaattc ttcccattga tcgggtgttt gcatgattac ttccgcctga
     6841 ttaggatttt gcaccatttt ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt
     6901 tgatagatca gataatagat cattcctgca agaacaagga agaaagacag aaagaagaaa
     6961 aagacccaac ggcgaaaact ctcagttctc ggtaaaaaac cgcttgtcgg ttctgcgata
     7021 cgtaaaatgg cttctaactg gccacttttt ttgactggta atgcgacata taaaagatcc
     7081 tcattcaaag tagtactttt gcgcaaagcg ctacctaggt tcccaccatt caatacggct
     7141 ttgatttcgg gacgggagtt tcgctgttca ttcaatgttt cattgtttgt gtcaaataag
     7201 atctttccag tagcatccat taaagtaatc cgttcgttcg actgatggac gaattcttcc
     7261 aaagcagttt tgttttgcgc tgcttggagg ttttcgacat ctaattgccg gatcagcagt
     7321 tgtgcttttt ttgtcaggta gctttcttgc tgttccacga tctgctgttg aaaataatgg
     7381 gaaatcaatt gccagccacc aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt
     7441 tcataaattt ttttccattt catcgttttg gttcctgaaa cttatagcca aatcctctaa
     7501 tagtcaccaa gtactttgga tgttttggat ctcgctcgat tttttctcgc aaatgactaa
     7561 cgtgtacatc tacgattcgg ctttgtcctg cgaagtcaaa attccaaatg cggtctagca
     7621 atgtgtctcg gtcgatcaca cggtcttttc gtttcatgaa ataaacaagt aattcaaatt
     7681 ctttcggtgt tagttcaatc agatggtcgt ctacggtcac ttgataattg gtcaaatcag
     7741 cagtgatttg tccaatcgat agatattcag gctcagcttc atcttttgat tgcttacgag
     7801 gttcaatccg tctgaatatt gctttcatac gggctaatac ttcgcgagga ctaaatggtt
     7861 tggtcaaata gtcgtcagca cctatttcta ggccaatgat ccgatccact tggtcatctt
     7921 tagccgtcag catcaaaatc ggtgtttcat ttttttcttg acgcagccgc tgagtgatct
     7981 ccatgccatc catcgttgga agcatcacat ctaaaatgat gaaatcaaat gattgttcta
     8041 atgctaattc taatccttcg cctccatctg tggcactggt taccttgtag ccttcttttt
     8101 ccaaattgaa cgtcaagagc gttacaatag aaggttcatc atcaacaact agtatttttt
     8161 tcattcaaat ctcctttaaa taggtttcat tctgtttata ttttagcata gaatcagtac
     8221 aaagggctat tttacaaaag aaataaatgg tataatagga aggcactttg ctgaatagaa
     8281 aggaagagca gtcaatgatt ggaatcagtg catgtttagg cggtctggcc tgtcgctatg
     8341 atggacagaa aaaagaagta ccagaattga tggaactagt tgaaactgga gaagctgtca
     8401 tggtctgtcc agaagtgatc ggcggattac caattcctag agatcccgct gaaatcattg
     8461 gtggaaatgg attcgatgtg tggaaagatc aagcaaaagt atggacgaca agcggcgaag
     8521 atgtaacgga agcgtacaaa aatggcgcga tcaaagccta tcaaaaattg caggaaaaac
     8581 acattgatct agtcatcatg aaggaaaaaa gcccttcatg cggaacccat tcgatttatg
     8641 acggaagttt ttccggaata aaaaaaaatg gtgccggagt ggctactgct tattttattc
     8701 agcaaaaaat gactgtgctc tctgatgaag aatggctgaa aactagaggt gagcagaatg
     8761 gaaatcgaaa agaccaatcg aatgaacgcg ctctttgaat tttattcgac cttattgaca
     8821 gaaaaacaaa tgaactacat ggaattatat tatgcagatg atttttcatt aggtgagatc
     8881 gcggaagaat acgaagtaag ccgacaagca gtatatgaca atatcaagcg gacaagcaag
     8941 atcttggagg attatgaaaa aaaactccat cttttttcta attatattgt tcgagaacag
     9001 gtattggaaa atatgactgc atatattaca gataagtacc cggaagataa aaaactactt
     9061 gaatatgtac agcaaattca agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg
     9121 catttgaaag cttaactgaa cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa
     9181 aagtatcaga agctgacgta aaagaaatga tgcgggaaat ccgccttgcc ttactagaag
     9241 ccgatgttaa cttgcaagta gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag
     9301 gggcagaagt gctggaaagt ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag
     9361 aattgacgat cacacttggt tctgaaacag cagagttaaa taaatcacca aaaataccaa
     9421 ctgttatcat gatggctggt cttcaagggg caggtaaaac gacctttact ggtaaattag
     9481 ccaattattt aaagaaaaat gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc
     9541 gaccagctgc gatcgaccaa ttgaaagtat taggtcaaca gctagacgtt cctgtatttg
     9601 atatgggaac tgaagtcagc ccggtagaga tcgtccgtca agggatggag ttagcaaaag
     9661 aaaagaaaaa cgactatgta ttgatcgata cggcaggacg tctgcatatc gatgaaacat
     9721 tgatggatga gttgaaacaa atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg
     9781 tcgatgccat gactggtcaa gatgccgtca acgtagctga tagcttcaac cagcaattag
     9841 gtattacagg ggtcgtcatc actaaacttg atggggatac tcgaggcggg gcagcactat
     9901 cgatccgctc agtgacaggc gcaccaatca aattcatcgg ttctggtgaa aaattgactg
     9961 acttagaagt attccaccca gatcgtatgg ctagccgtat ccttggtatg ggggatatgc
    10021 tgacattgat tgaaaaagcg cagcaagact acgacgagaa aaaagcggaa gaacttgcac
    10081 agaaaatgcg ggaaaattca ttcgatttca atgactttat tgaacagctt gatcaagtga
    10141 tgggtatggg cccactggaa gatctaatca aaatgatccc aggaatgaat caagtccctg
    10201 gaatcgaaaa cgtcaaggta gatccaaaag atgttgaacg caaaaaagcg atggtttatt
    10261 ctatgacgcc agcagaaaga gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa
    10321 ttgctgccgg ttctggaaac agtgttgttg aagtgaatcg aatgatcaaa caattcaagg
    10381 aatcaagaaa aatgatgcag caaatgtcca aaggggatat gaacatccca ggtatggacc
    10441 aaatgtttgg ttcaggcgtc aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca
    10501 agaaaaataa gaagaaaaag aaaaaacgga aataagatga aaaaaacagc ttcggtacag
    10561 actgaaaatg tctactgccg gagcttttct atcagcaatt tttattcttt actaatacaa
    10621 ttactctaaa cgaagaggtg gcacagatga gatgtccgtg gagtgaacaa acagaaagta
    10681 tgcggcaata tcatgatact gtctggggtg ttcctgaata tgatgaccaa aagctgttcc
    10741 gaaaattgat gcttgatatt aatcaagccg gattgagctg gcagacgatt ttaaataaaa
    10801 gtgaagcatt cgatgaagca tatgatcgat tcgatatcaa aaaagtcgca aattatggag
    10861 aaacaaaaat cagtgaacta atggaaaaca aaggaattat tcgtaaccga agaaaaatcg
    10921 aagcagccgt ccaaaacgcc aaagcagtct tgaaaattca agaagaaatc ggcagttttt
    10981 cagagtatct ttggtcgttt tctaatggaa aaatcgttga ttcttcttat cattcacaag
    11041 aagaagtccc aacaacaagt cctctttcag atgaattagc aaaagatcta aagaaaagag
    11101 gatttaaatt tatcggaagc acaacaatct atgctttttt agaagctgtc gggatcatca
    11161 atgatcacct tgacagttgt ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg
    11221 aataaagcat gcgaaaaatc atattttatg gtgctataag tttagatggc tatttggcaa
    11281 cgaaaacgga tgatcttcaa tggctttttg acactccaac aggtaagcaa actacttatg
    11341 aagcttttta tgaaacaatc gatacgacca tcatgggcag aaaaacttat caggaagcaa
    11401 aaaaatattt ggagaccgaa gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc
    11461 acgcacaatt acaattgcca gatgctgagg tcgtaaacga agatcctgtt gcttttctaa
    11521 aaaatctaca aatgacagaa ggaaaagata tctgggttgt gggtggcggt cgattgatca
    11581 aaccgctatt ggaacatcaa atgattgatg aatggtatat ccagattacc cctgtattat
    11641 taggggacgg gattccttta ttccaaaaag gaacatatga agcgcgtttt gaaatgctag
    11701 acacgacacg attcggtgaa tttatcgaat tgcattatgt acgaaagtag aagtaaataa
    11761 aataaaaaag gcaactatat gcagaaatgt cctgtacata gttgcctttt ttattaccgt
    11821 tatgcttttt actctgttaa tttgttagaa aaatctagat acgcatctaa aatcgtttgg
    11881 aagaaactta atgttccttc aacgatatta ccattttcat caattagatt cgcaacgttt
    11941 ccgatatatg cttccggttg ctgcaaggtt ggcacattca aaaagacaag tgattggcgt
    12001 agatggtggt ttgcgccaaa gccgccgatc ccacctggtg atgcagtaac gactaaacct
    12061 ggtttatgat cccaaacact gtgtccataa ggtcttgagc ctacatctaa agcatttttc
    12121 aatactgctg gcacagaacg gttgtattca ggagtaacga aaacgacacc gtctagtccc
    12181 ctcacttcct cacgaaaacg agtccattca gcaggagcct gttctggagt ttcgagatcc
    12241 tcattgtaaa atggcagatc atcgattttt acaaatacac tttcgtaccc ttcaggaagc
    12301 aatttcgcaa aagcttctgc tacttttttg ttgtaagaat cttttcttaa actaccaata
    12361 aaaaatccaa ttttttttgt catacgattt ttcctccttc aaactatcta taataatctt
    12421 acaaaagata agtaactgga gcaaataaaa acacttactg acaaaagcta catagaagat
    12481 agattactta tttttaacaa aaaagaagtc taaaatcttc tattttctaa aaaaaatcga
    12541 atttaatcaa gctgtaaaga aaaaaagctt tacagggtaa aaaaaaactg ttacactaca
    12601 tcttgtgaat aaaacaatgg aggtggaaat aaataatggc agttaaaatc cgtttaaaac
    12661 gtatgggttc taaaaagagt ccgttttacc gtatcgtcgt agctgattca cgttctcctc
    12721 gtgatggacg tttcatcgaa actgtaggta cttacaatcc tttgaaagac cctgcagaag
    12781 tagttttaaa agaagactta gttcttgact ggttatcaaa aggtgcacaa ccttctgaca
    12841 ctgtacgtaa catcctttca aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct
    12901 caaagaaata aggtgaccgg atatgaaaga tttaagcgac ttagtcttaa caatcgttcg
    12961 tccgctagtc acttatcctg atcaagtcaa gttagacgtc gtagagtcga aagactttta
    13021 cgagtacaat ctaacagtgg ctcctgaaga tattggccgt atcattggaa aacaagggcg
    13081 tgttgctaaa gcaatccgca caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt
    13141 gcgtttgaat atcatcgatg ataaggaaaa gtaaataaac gtgctgtact cgaattttcg
    13201 agtgcagctt ttttgttttt tttattaatt gaagaaatga ttatttggca aaacaagcaa
    13261 gaaagcgtat ccttatagga ggagggagtg aacagccaat gagaaagaaa atgttattta
    13321 ttccatttgc tctattgtta agcgcttgta gttcggttac tcaagaatca gacaatacac
    13381 atctatcaag cagtatatct gaaactgtta ccagtccaga aaaaagtagt accaaaacgg
    13441 cttccaccac tgaacaaaca atggctacct caaataatga gaaaaagact gctttagacc
    13501 agttgaaaga acaacagcca aatgttccca tgcctctaga tgtaccagtc tcttcaggct
    13561 atctcaatat cgcagcgaca catacaaagc aaggttattc tattttatat tacagaacag
    13621 atcgtcccct cgggttgaat gctgacgaat tgaatcaaga aacgcctatt gcaacctatc
    13681 tatatcaata tgggtttgct tctagtcaag aaacaatcca agttttgcag ccatttgaga
    13741 tcgacacaaa tggtcagcaa gtggacttag gttctcgaat cacagggtat cagcaaggtg
    13801 cagcaggttc aagttttctt gaatggcaag aaggaaactg gcgtattcga atccgtggga
    13861 ataatataga aggccaagat cccttactat tagctaaaga aatcgtagct tatttggaag
    13921 aaaacagtct accagcacct gaacaatttg gtaaaattac tgttgatatg ggtgatacga
    13981 cgaatcgagc cgtagaagta agctggcaag aaccgaaaaa tgcctatacg atcactcatc
    14041 aagatcctat gtctgcgttg aagatggcag tttctatgaa gaggttgtga aatcagcgcg
    14101 ctgatctgag cccatcattt aactccaagt cacaggaagt aatcaaaaat agcacgccgc
    14161 atttttgcat aattttggct taatacctta aaagaaaact agaattgcct cttcacacat
    14221 gctaaaataa aacagacaaa aaagaaatgg ggatataagt tgactgaata cttgaatgta
    14281 ggaaaaatcg taaacacaca aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat
    14341 ttcccagaag aacgttacaa aaaaggaacg gtgctaacac tattccaaga cggaaaagca
    14401 cctgtggaat taacagtcaa aagtcatcgc aaacataaaa attttgactt attgagtttt
    14461 gaaggacatc cttctatcaa tgatgtggaa aaatatcggg atggcatatt aaaagtgtca
    14521 aaagacaact ctgtagatct agctgaaaat gaattctatt accatgagat catcggtctg
    14581 catgtattag aagatggaac tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc
    14641 aatgatgttt gggtcgttca acgtccgaaa aaagctgata tattgcttcc atatatcgct
    14701 tctgtcgtca aagaagtcga tttagaagct ggagtggtcc gtgtagaaat tccagaagga
    14761 ctgattgacg atgaagattg atgtattgac tttatttcca agaatgtttg aaggtcctat
    14821 gggggaatcc atcatcggta aggctgtaga taaaggatta ttagatatca atatctctaa
    14881 cttccgcgat tattcagata acaaacatca gacagtagat gattatccat acggcggtgg
    14941 agcaggtatg ctgctcaaag tccagcccat ctatgataac atccaagcca ttgaaaaagc
    15001 tgcaccggat gtcaaaaaaa gagtaattct tcttgaccca gctggtaaac gattcgatca
    15061 gaaaatggca gaagaatttt cacaagaaga acacttagta ttcatttgtg gtcattatga
    15121 gggatatgat gaacgtatcc gcacattagt gacagatgaa gtttccttag gagattatgt
    15181 ccttactggg ggagaattag gtgcgatggt catgatcgat gcaacagttc ggcttttgcc
    15241 agatgtgtta gggaatcaga cttcagctca aacggattct tattcaaccg gtttattgga
    15301 acatccccaa tatactcgcc cggcagaata taaagggatg aaagtaccag aagtattgac
    15361 aaatggaaat cataaattga ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta
    15421 tcaacgtcgg cctgatcttt tggaaacact tgaatggaca cctcaaatga cgaaattttt
    15481 agaagaaatc aagaaagaag aacaagaaaa aacagcagat gaagccaagt gactttttct
    15541 aaaatcttct ttacagtagg ttgataatat gttagtattt tatggtgagt gcaaaagcac
    15601 ttaactatta cgatattccg ctgtgagaaa agctcataag aatatttgga ataaggagaa
    15661 gaaaaccatg aatccattaa tcgaagaatt aacaaaagaa caattacgtt ctgacatccc
    15721 agcgttccgc cctggtgaca ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga
    15781 acgtatccag ttatttgaag gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac
    15841 ttatacagta cgtaaagttt ctaacggagt gggcgttgaa cgtacattcc cattgcatac
    15901 accacgtgtt gcaaaaatcg aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta
    15961 ctacctacgt gcattacacg gaaaagctgc tcgtatcaaa gaaatccgtc gttaatcatc
    16021 gttatcagac agagaaagaa cccttggtat tcaagggttc tttttttatt atctgttttt
    16081 ttatattaac gatttctatt tggtacattt ttgattaata aaaaggggca aaaaaggggc
    16141 aacaagttac agtttttcta tatcttcttt caatttttct tttactcctt ttgtcacatg
    16201 caaataaatt ttttcagtga tatcactatt ttcgtgacca acgcgatcct gaatggcata
    16261 cagaggagtt cctagttccg ctaattttga aatatgggta tgtctaaaaa tatgtgaact
    16321 aagtttttta tcaatcttca tgtcagcttt atgacttctc aaataggtgt taattgctgt
    16381 tagttggaaa ggagttcctt tcgttgtttg aaatagaaat tgaccatttg gatttaattc
    16441 tagaagctca ttgtaaatag ctatcgcttt ttttggtaaa tcaatttctc gcattccggc
    16501 agctgttttt gtagaatcag attttttcat atcagctatt gaacgttctc tatacatcat
    16561 cgtcccattt ataactactg atgcattatt attggtaatg tgtacatcat ctttacttaa
    16621 tgcgattgct tcaccagacc tcatcccagt caaatatagc cactggaaaa ggagagaata
    16681 tcttttattg tgtgaagttg tgaaatctac tagtctatta tattcatcat cttctaaaaa
    16741 tttatcttta atttttatcg tttttgactc tcttttataa tcaataacta cttcatcgat
    16801 aggatttttt tctacataac cttttttcat agcatatgaa aaaagaagat tcaatttgga
    16861 tttaattaca ctgacgtact tatttgacaa gtcatcttta tatatcatgt cttcaaaaat
    16921 attattcaaa tctattgtat taataccaga aacgatgtaa gtctcaggta tcttcttctt
    16981 tatagtgttt aatatattat tcgtaggata atatgtagat tccttaactt gtcttttgta
    17041 tataactaac cattcttcaa caagctcatg aaatgttaga tcaggcttat gaagtgtttt
    17101 ttcgtttaat tttatatcaa tttttttatt taactctagc aatgccactt tctgagtttc
    17161 tcgagattta tttttataag taatgctcac tttttttctt tttctagttt ttggatcaat
    17221 atatcgctca ttatatttat atactttttt tccgttttta tcagttttag tttcgatcca
    17281 cattgttttc atctcctatt tgttgctata ataggaatag ataagtaagc cgattatagc
    17341 aggtttattt ttcatcacgt ccacaaactt tggcgaggga ggggcgtgtt tttgttagtt
    17401 attgtggtag gttatctaaa gcgtattgtg cttgctcatt tgtaaaacct tcaaatataa
    17461 gttgatcgta caattgcgaa tctgacattg atgcccaatc atcgtaactt tccgctttag
    17521 ccaaagcttg ttcgttgtaa tcgacatcaa cattagccag cgcataatcg attgcatcac
    17581 ttggatattc ttcaaactct agttgttcac gcaaaccttg ttcagacata ccagcccaac
    17641 ctagatagct ttcagctttg ctcaatgcat ttctgtattc acgaggaaca ctttcttctt
    17701 gagtaacttc ttctatagta gtttcagtag tttcagtttc ttcttcaacg actgtagatt
    17761 ctgctaccga ttctgcagaa gacacttctg tcacgctttc tttagttgtt tcagcgacgg
    17821 tatttttagc atcatcactg cctccttgcg aacctatagc aataaacacc acaacaaccg
    17881 ctaacaacca aaaccatacc cgtttataaa acggtttctt aactttatac attttcccat
    17941 cttgacccat aacttttttt gccatttaaa tatccctcgc ttcttgttat aatatatttg
    18001 tgatctcaga aatgaggtat gagtccgtgt tgcagcacgg gcttttttct ttataacttt
    18061 tttagagatt ataggcaaaa tagtagggca taaaaatgta ttattgaatt ccgtatttag
    18121 aaaatcctaa ttgaacttca ccggaagtct tttgctgtgt tgtacgcaat gcttcttctt
    18181 cagacattcc attctgtact ttccatgcaa caggcgacat cccgtatttg ttaacaaaat
    18241 cagtaagtga taaagtgtca gcgtcttgct gagcgcttgt ttgttggtct tctggattct
    18301 gttgagatgc tgcttgttgt tctttctgat cttgactgat aatattgcca gcatcatctg
    18361 tagtcaatcc attttcataa agggccacgc cgaaagcttc ccactctttg ttggaccaat
    18421 ttgcacgatc agctggagtt gactgtaaag tgcgttgttt catctgttca tatgtttctt
    18481 cttgaggtgc ggtttggatt gtactctgac tggagcttat aactgttggg ctaggttccg
    18541 ccgtagcttg gttggagctt gtaactgttg aactagaatc tgttttagat gtagatttgc
    18601 tagtagatga actggtttca gttgtttctt ttgttttact tatttttgtt tcttggttag
    18661 aagtggtatc tgttgattca gcttttttat tatttgaaca agctgaaagt agcagagcag
    18721 tacttaacaa caacataacg ctaacttttt tcattttata attcctcttt ctcgttgtaa
    18781 tatgtgtgct aacacgggct tttttatata agaaaacgat aagcgctttc tggaagcccg
    18841 taaagattct ttaattcctc gattttttta ggatattgat cattatcttc tttataaaga
    18901 gaaacaatga gattagcagc aaagcaatta gcttcgcttt cagatttgct tctagatgtt
    18961 cttgttgata cataatagct ggataagcca cgatgaaaaa tagcgtgacc taattcgtga
    19021 gcgcaaatgt agaatctttc ctcagagtct cgcagttcat catttaaaaa gattatcgca
    19081 cgatctctaa tttcttgaaa ctgccccttg ggattctcga taaaaggaac gtattgaatt
    19141 ttaatgccca tcttttcaca aatataaaaa ggattagcgg actggtattt ccgcttcaac
    19201 tcctcgacta aattaatcgt atccatctcc ataagctcac atctttttgc ctttttcttt
    19261 gtcttctttc acaatatccc agaaagtcgc tatcaggata tcttttacgc gctgtatttg
    19321 ttcgggtgtc aatgtttccc caccataaga catattaaca tttgagtcta gtagtttatc
    19381 aagttcaacc acttcctctt ttgtagccca cttgggaaca ttattatttc ccaataaata
    19441 atcagttgtg acaccaaaat aatcagcaac tttctttaag ttctcagatt ttggcgaggc
    19501 tttatcccat cttcttattt gtccattaga gatgcccacc tgtctttcta cttctgctat
    19561 agtcacatgc ttttcgtctg ctaattcttt aatcttagta actaaactca ttattatcaa
    19621 cctttcaaag ctgaaaagaa aataaatagc ttaaaagtta tatttttggt tgacaattag
    19681 cttttaagct agtatattta attcgtaagc taaattgtta gctaaataag agcaacaaaa
    19741 aactctacta atttaaaaca ttctctcggt cgccaaactt agaaatgtta ttttagaggc
    19801 tttttataag tcttatttaa ctatgtattc ataatagctt aaaagctaat aggtgtcaac
    19861 gatttagcta attttttagc ttacaaatta tttgtttaga aaggagctat ttttatgtct
    19921 gagaatttag acttaaaaat tcgagcggag atgagaaaaa gaagaatgac tttcaaagaa
    19981 ctagctgcgc ttatcggtat ttcaggagct tatttatcag atattctaaa cggcaatcgt
    20041 gatggaaaga aagcacaaca gcatatcgaa acagtgaaaa aaatattgga catccgatag
    20101 ggggggtagg cgatgacaaa actaaaaaaa caagattttg taaaaaaata taattattct
    20161 ccatctactt atcaacgtcg aatgtcggaa ctaaaaaata cagcaatttt ctcagcggcg
    20221 tatgaacggg tcacaggaca agaagtttgg atcaatacag aattatacga taaatttttg
    20281 tctttcaaat cctataacag gttacgcaca agaaaggtaa cacctaaaga atttatcgag
    20341 aagcatttag ttgatttata aaaaacagaa cattttgaga ggtgaaggtt aatgggtaaa
    20401 ttcaacagag cattagtatt cagcgcaccg ctaatcgtct atgctttagg gctttgggga
    20461 agtagacaag cgttgatagg aacgattgtg tacatggttt ggatttttat agggctggat
    20521 gaagctgagt acaaaacaaa aaagccagtc gggagggact gactaatgag taaggacgat
    20581 attgtatttc tgttaattat gttttgtggg attggatcaa taataattaa tttgtatttg
    20641 attattaaag aaaaattttt caattaaaat gagtctttta gctttaactt aaattttttt
    20701 tgataattag ctgttcttat gaccattaca agtttttcac caggtaatat atctttcata
    20761 tctaaaactt ctgaaaaagc gactgagtag ccaggagaga ttgcttgtgc caacggtata
    20821 ggagataaaa ggctactttt atgaatgctt cccatatcaa aaccattggc gtgataatgt
    20881 gaacaattaa ttttcttacc gttgtaaaga tacgtacatt cgataattga gtttggcatt
    20941 tttgatgggt ttgcaattac gccagtaaga atcattcgat tacttctatc atgcttaact
    21001 aaaaaatagg agagcgtaat ttttctttta tctttaaata agttatagag ggataaaaaa
    21061 aatcctgaaa tagaaatgat taaagtaata gcgacttggt aatcgctcaa aatttttatc
    21121 aagtaattca ccaccttttt actcaaatta tatcaaaaag gagagaagaa ataatgcaag
    21181 aattagtaat tttgaaaaat aaaaaaagac acgaacgccg gcaagcatga tcgtgtcagg
    21241 gaaataaact atctttctat attttaccac agaaaggaat gaaccgtaaa tgctaattgc
    21301 aacggataca ctagaccgca tctttttaaa agacgaatac aaactgcgca atatagatgc
    21361 gtcaggaatt ttagtatttg atctttatga caatggaaaa attggtatct atcaagcaag
    21421 tgatattgaa gaaacaaacc ttgctttcga acaaattgat gattctgtgg aattggattt
    21481 agatgaggca atcctagctt ttgaacaaat tgcaaaatta ttaaaggagg cacaaaagaa
    21541 tggcaactct ttaccaactc agcgagtcat atatcaaagt cctggaactg gcagaacaat
    21601 tggatgaaga aattcttcgc gatactctcg attcgattaa tgaagcgatc gagtataagg
    21661 cagaaaactt agcaaaaata gttaaagaag tagaagggaa agctgagtta atcgatagtg
    21721 aaattaaacg tctgcaggaa cgtaagacat cgcttttgaa caatgctaag agtatcaagc
    21781 actatttaca agaggaaatg gaaaagactg gtaaaacgaa gattaaaggt gaattattca
    21841 acattggaat tcaaaataat ccagtatcgg tcaatgtaat caatgagaac ttaattccaa
    21901 aaggattctt tacccctgtg cctcccaaat tggataaaaa gcaattgaag gaggaactga
    21961 agcacggaga tattcctggt gctgaactcg tccaaacaaa aggtttgagg ataagatgat
    22021 gaaggaggag aaataatgga acacgaaaac gtaaaagacg ctttgaaagc tgctatcgaa
    22081 atcgcggaag ctaaaggaat aaaggttgac ggcaaaccag caactgtcca tgacattcaa
    22141 aatttgacga aagaacatct ttattttatc gccgacttgc ttggtttgtc ggaattgtat
    22201 ttagacaaat aaccccacac ggaaggagtg gctagggtgg ttgtattaca agtaatagtc
    22261 tgcgtaacat tcatttggtc gtgcataccg gtttgtatat atatcatgaa gaagcttcgc
    22321 cctgagtctt acaaattata cgcaatttac agcataacag tagctcttgc atttacattg
    22381 tttgcaattt ctaggcttcc gttttaatcg tgggaatgaa tttttaatga actaaattat
    22441 ctataaagca aaaaaaggag aaacaacatt gaaaaacaaa ctagcaaaaa caacagcaat
    22501 catcggacta gcactaggta gcggcgttat cggctacgca gcaagcaatg cgtttcaaga
    22561 tttggacacg atcaaagcaa atttcaacac agtcctacaa tacggtcaaa cgaaatcgca
    22621 acgtgtgtca gaacttgaat cacagctatc caacaacact cgcacacagg agcagctgaa
    22681 agccgaaatt gagcaaatca aatcggacaa gcagaaggaa attgaagcca agcaacgaga
    22741 agtcgaacaa aagcaacagg agatcgctac aaagcaacag gaagccgata gcttgcgcca
    22801 acaactatcc acagtgcaaa acgacaagga gcagttagaa cagcgcgtga gcgagttacg
    22861 gcagtatacg gatcaaaagg taggggagtt ggggaaatga aagagttgac tatttatgac
    22921 ccaaatattt catgggctaa acacacaatt aaagtttctt ttatgatttg gggttacaaa
    22981 ggatatgtta cttacaaagt gggcggtaac actaagggtt tatcattaat agctattgat
    23041 tcagatgatc tatatgatgc taatttcgag gataacccag ttaatttccg tgaccttgat
    23101 gaagattggt tctctatgga acttactaac gataaaggcg acagtacact tgtagaagat
    23161 gaattcgaca gactaggtga ttatattgtc ggtgttgaaa ttattgcgca cgaaccagag
    23221 taggagagtg aatacaaaat gattaaaaga atcactatat cagttgaaga agatagtgga
    23281 tcaacaatgc agcataccga caatcgaaaa acttttttaa ccaatagaaa ggagaatttt
    23341 gatgcagatt aagaaggcga cggatatcga acaaaccaag ggaacatatt tgatctacgg
    23401 tgctccagga aaaggtaaaa cttcaacggc taaatatttt ccagggaaaa cactgatcct
    23461 tgatattgat cggacttcaa aagtccttag aggtgagaaa aatgtcgata tcgtatatat
    23521 cgacaacgaa gatacctgga acgactgggg caacacgttg gctgatttaa ctaccaatta
    23581 tgtaggtgtt tatgacaacg tggttgtcga taatgtcagc gaattggaac ggtgtattct
    23641 gtccagttta ggcgcagaag gaaaaaacaa cggcgttcct tcgcaaggcg attatcaata
    23701 tatgcaattt agaatggtga acagcctacg ctatatgaaa aatttagaca gcaaccttat
    23761 ctggaccgca tgggaagaaa tcgatttgtg gacggattcg gatggatctt cttacaacat
    23821 cgctttgccg cagatcaatc gaaagattcg aaacaatatt ttgggattat gtgacgttgt
    23881 tggtcgattg atggtaaagg aagacgatga acgcggattt attttacaag ccacagattc
    23941 aacctatgcc aaaaatcaat tggataatag atctggctgt aagcaaagtg agctgatttt
    24001 agatggaatt gtacgactac cagaatgatt tagtcgatcg tgcaagacaa gcctatgttg
    24061 atggttacaa ggcaccatgt atcgtatcac cgtgcggttc tggaaaatca gtcatggttg
    24121 cagagatcgc taaacgaacc acacgaaaag gcaacagagt gttgttcttg gtccatcgtc
    24181 gtgaattgat tgatcagatc aaagatacgt ttcaaaagat gggcgtagat aatcagcttg
    24241 taaatttcgg catggttcaa acagtcgtac gacatcttaa aaccatcaag aaaccacaac
    24301 tgatcatcac tgacgaaaac catcacgggt tagcagcgtc ctacagaaaa atctatgagt
    24361 attattcgga cgttccgcga ttaggtttta ccgcgacacc catccggtta aacggtagtg
    24421 ggttggggga tgtgaacgac ttgctaattg aaggagtatc ggctaaatgg ttgattgaga
    24481 atcatagatt agctccttat gaatattatg cacctaaatt gatcgataca gccgaattaa
    24541 agaaagcttc gactggagat tttacaaaga aatcaatgga taaggcagtg aagaatacta
    24601 tttatggcga cgtgttgaaa cattataaaa cgttagcaga aggtgaacag gcaatagcat
    24661 actgtcacag tatcgatgca agtaaacaca ctgctgaaat attcaacgat gctggttata
    24721 aagcaacaca tatcgatgca aaaactccaa aagatgaacg tgcagatatt atcgaagcgt
    24781 tcagaaatca tgaaattaaa atcctatgca atgttgattt gatcggagaa gggtttgatg
    24841 ttcctgattg ttcgactgta attatgttga gacctactca atcgttatca ttgtacattc
    24901 agcaatcgat gagggggatg cgttaccgac cagataaaac gtcaatcatc attgaccatg
    24961 tgggtaatgt gaaccgtttt ggattgcctg atatggatcg tgaatggagt ttagatacaa
    25021 aaaagaaaac aaaaactgat agtgatttat ctatcgttca atgcgcgttt tgttttggtg
    25081 cttacgaacg gccaaaaggc gataatattt gtccgtattg cggagagttg cagccgattg
    25141 aagaacgaaa gtcagagtta gaaatcgatg aatcggctga attaatgaaa gtcggagaaa
    25201 cgaaaattac tttgaatttt gagaacaata aatattacaa catgacggaa gatgaagcag
    25261 aaagtataga ggacttatat gccatagcaa gagctaaagg atttaaacct ggttgggctt
    25321 atatggctgc taagaggaag gggtggttgt aggaatgggc ggtatttttg gtaaaaaagc
    25381 agaagatgtt caaggttcaa taataaatgg cgtaacaata ataggtgata ctggaaaaag
    25441 agatagttca ggcaatcaaa ttgtaatcgc tagaaataaa aataacgaag tttttgaatc
    25501 ttctctatct aatataaaaa gaggtgttgt taacgggaca ggcgaagaga gtggctacgt
    25561 taatttaaaa aaagctaatg aaaaatacca tgttgaagga acttacgtta aggctttgtc
    25621 tgtcggtgga acaaaaggcg ttaattggga taaacgaaga aataaatgga aagcacgatt
    25681 atatttcaaa ggagaatatg tcttacaaaa ggatttcaac accaaagaag aagcaatcca
    25741 agcacgactt gcagcagaag aaaaatattt caaacctatc ttagaaaaat atgaaaataa
    25801 aaaggagaat gattaacatg acaggattta acttagactt ttcaaaagcg caacaaggga
    25861 acgaaatcaa agacggtacg tacgaagtgg tcgtaaacaa agctgtagaa aatgcaacta
    25921 aatcaggcgc agaatttatc gatatcgatt tgatcgttcg taacgatgtg gatcaaccgt
    25981 tccaaaataa acacattttc gcaaaaatct ggaaagccaa agctaccggc aaatataacg
    26041 aaggtatgat catggcaatt gcacaagcgc tacagttgga agacggaaaa tcatataacg
    26101 ggtttgatga attgctggcg gactttgttt tgaaaaccgc ctcagtacgc gtgaaaactg
    26161 aagagtcaaa tggatataaa aacgtcaatg tgaagtcttg ggataaaacg aacacacggg
    26221 gcgtaatgaa tcaccaattc aaaaatggtg atgaaccatc cttcggaccg gaacgttcaa
    26281 gagcgacaac ggttagaaat gacaatcttc cattttaatt gaggtgtaat caatgtatga
    26341 aaaaattcca aaagagctta aaaatctcaa acaatggtgt gtttataagc tagtttggga
    26401 tgaaaagcgg cagaagcaca cgaagatccc ttataacgcc aacaacgggc ataaagccaa
    26461 atccaatgac gagagtacct ggtctgattt tcagaccgct ctcgctgcta ttgatcacta
    26521 cggaatgagt ggactggggt tctttttcaa accaccttat ttcggcattg atatcgataa
    26581 tgctgaaggt gaagttgagc gatttaaatc aggtgatata gaagaaaata ttatctatga
    26641 atttatcgaa agcatgagat cctatgctga gtattctcaa tcaggtactg gcatccacat
    26701 tattgctcgc ggagaattgc ccggcggaag acgcaggaaa ggtgacgtag aaatgtacca
    26761 gaacggccga ttcttcgtta tgactggaaa tgccgcatca aaatatttag aaataactga
    26821 gccgaatcca aaggatatca aacgcctata tgatcgctac gttggagatg aaaaaatcat
    26881 ccaattcaaa gaagaaaatc cgctgatgaa taccgttgat ttaccggttg aagaaattat
    26941 caaacgtgct gaatcgtctt cccaaggggc aagattcaaa gtctttatga acggcggttg
    27001 ggaagctttt tattcctcgc aatccgaagc ggatatggcg ttcgccaacg acttggcttt
    27061 ttggaccggt agagactttg aaaaaatgga tgagattttc agagcgtctg caatgatccg
    27121 tagcaaatat gaccaaaaaa ggggatcggc aacttacgga gaatctctac taaataaggc
    27181 aattgctgat acaaattccg tctacaaccc caaacggaaa tctgatttta agatttttat
    27241 caaagaccag gaacagccaa aagaggaaaa atactattcg tacgacgaca ccggcaacgc
    27301 ggatcggttt actgatatct acggaacgtt agtcaaatat tcgtacattg ataaatcttg
    27361 gtattactac gatgggaaag tttggctaca ggacaatacc ggcgaagttc ggaaaatgat
    27421 cgatacgaca gtagacatca tgggaaaaga accgttaacg atccctgaag gcgcagacga
    27481 tgaaaccaaa gaagctttga tgaaagcgaa agaaaaacac gtcaagcgtt ctcgcagcaa
    27541 tgccggaaaa aacgctatga tggacgaatt gaaacatcga ctatccgtat tgccggaaga
    27601 gttcgacaaa gataaaacgc tgttcaacac acaaagcggc tatctgtctt tgcatgacgg
    27661 tcttctacac gagcatgaga tagacaaaat gtttacacgt gtttcaaatg tcgagtacac
    27721 agaatcggtc gattgtccta tgtgggaaga atttattaat cagatttttg acaatgatca
    27781 agaattaatc agatatatcc agaaatgtgt cggatattcc ctgaccggat ctaccagaga
    27841 gcaatgtatg ttcatcctct acggtcatgg gtcaaatgga aagtcggtat ttttggaaat
    27901 tatttctgaa ttgatgggaa actatgcgat gactatgcag gcacaaacga tcatggtcaa
    27961 gcaaagccaa tcatcagcaa acagtgatat tgcacgattg aaaggtgcgc ggttggtcac
    28021 ttcgtcagaa ccgaatgaag gcgtccgact ggacgaaggt ctggtcaaac aattgactgg
    28081 tggtgataaa gtcacggctc ggcaccttta tggaaaagaa ttcgagttcg aacctgaatt
    28141 caagttgtgg ctggcaacga accacaagcc catcatccgt gggaccgatg acggcatctg
    28201 gcgacggtta aatttaattc cattcactgt ccaaatcccg gatcacaaaa aggataaaaa
    28261 tctgaaattt aaattgcaga cggagttgca agggatcctt aaatgggcaa tcgatggttg
    28321 cctgttgtgg cagagagagg gcttggagaa accaaagtcg gttgttgcag cgagtcagga
    28381 ttatcgaaac gaaatggatc aaataggtac atttatcgaa acgtgttgcg aaacaggtcc
    28441 aggactaaaa atatcaggcg gagaattata caaagtttat cgtgaatggg catcagataa
    28501 tggcgagcat actttcacta acacgaagtt tggcagagag atttcaaaga aatatagcaa
    28561 agaaaaagct ggtggattta tggtttataa aggaatcaca ttaaaaccaa gaaaatacga
    28621 taacgttaga gaacttttta aataaatctg gagggttctg gagggttgag aacgaaaccc
    28681 tccaaccctt agaaacgttg atatatcagt atttatatta tatttttctt tctttctgga
    28741 gggttttaaa aaaataataa agtattaaag taaaaaataa aaagtaaatt atataaaaaa
    28801 gaataggttt ttgggtctga ccctccaaac tctccaccaa aatcttccta aatgctttga
    28861 taccaacgat taaacagtct ggagggttac attgaaatca gaacatcaaa tacaagacga
    28921 gatcagaatt gccttatcaa aacatggttg cacgattttt cgatccaatg cgggacaagt
    28981 gacaacgatc gaagggcggc aattttacgg gatgccaaaa gggttcccag atctatgtgg
    29041 acatcgtaat tctgatggga aagcaattta tatagaagta aaaaatgaac gcggtaagtt
    29101 gcgtgaagat caaaaaaggt ttgctgaatt tttaaacggt caacctgtac tatacggtgt
    29161 tgcacgatca gtggaagaag cattgaaaat tgtggaggaa tcatcatgac aacagaagaa
    29221 gtgattcaaa tgcgtattcg aagccttcag cgtgagattg acgatctgga acggacaaag
    29281 gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt tgcacataga gaatttaaga
    29341 agggaaatcc atcgattgga ggaatgagcg tggataagaa agcagcaatg aaacgaatca
    29401 tcgaactgac acattctgag aattggcaag aagacaaaga aatagttgca gaagtccaaa
    29461 agctcggtaa atcaatgtgg agtgaaaaaa ccaaacggag aacaccgaga aaaattgcaa
    29521 tctggcatgg tgaccgaatt ctagtaacag gtactgctga acagttatct gaaattactg
    29581 gattaagcaa aaacattatc tgggatagag ctaaacgcga gaacgttgat tcgaaaggtc
    29641 gtcaatttaa acattgggag aagaaataat ggaggaacta atcacaaaag tagagcagtg
    29701 ggcaaaagat aagggattgg atcaagctga ttccagcaag caaatgttaa aaacgatcga
    29761 agagattggg gaagttgccg cttctctagc tagaaaagat gaatatggtt taagagacgg
    29821 aattggagac gtagtagtaa ccttgattat tttagctatg caaaatgata tggatttgta
    29881 cgagtgtctg aaccaagcat acaacgaaat caaaggacgc acaggaaaaa tggtagatgg
    29941 tgtattcgtg aagtcgagtg atttggagga cagcgatgaa taaaaaagta ttaattgata
    30001 aacaagcatt gattgatgaa ttaatgaaaa tacctggtgt gggatctaat agtgacgctt
    30061 tagaaacgat taaacgtttc ccatcgtatg aaccgcagaa gccagttgtg cctaagtttg
    30121 tggcggaact actcgactat tatcgtcagt caacagatgt tgatttatta gctttattga
    30181 taactttcca tgattggtat tatcgcaaaa ctaaagacgg tgagcatgaa gaagcaattg
    30241 attggctagt tgatcatcct gaaatttata tgcgtgcatg gctgtttggc tacgagctcg
    30301 agaaagagcc gttgtattgc gtaataatca atggtagata tcttgtcaaa gtattcagca
    30361 atacaaacgt tgtcatatta gttccagcag atgaattttc acagtacgtt actcaaaaat
    30421 ttcaattaac tgaaaaacaa atcaaagcaa ttgatgaaag atactggccg tttgctgtgc
    30481 cagtggatga ggtggtagaa ggatgagcga cccatacatt actaaattag aaaagttgtc
    30541 cgaagatttt gagaagaata aaaataaact aactaaagat aaacttatag aactggttag
    30601 gttggttatc agaggagcta ttacaagaga aaaggcgcag caaaacatag aaatttttgc
    30661 tcgtagtgtc cgagatggat tagagaagaa agttaaacaa aatgaacaac agacaccgca
    30721 gaataacaaa actaagaaaa cgggaactga atgtactaaa gacaaagttt gaaaaagaat
    30781 atggaatttc agcagaagaa acatataaag tggcaagtca gtgtgttgct gatgcgagcg
    30841 gcgctattcg taagtttgga atttcgatat taagtgatga tcgtaaatgg gaggaaaaag
    30901 aatgaaacta aaagacggat tttactccag cagtcatggt atcggcggtt taatgctaga
    30961 tatgccgaca aagaacccta aaacacgtga gaaaccaaaa ttcaaagtcg gtgacatggt
    31021 tcgctgtgaa gcagaaggat tcatctatcc attttgtgga tatgtagaac atctctataa
    31081 tcactcagca atcattcgta ttgaaaacac gatggaatgt gacaagtggt tagcgaaaag
    31141 caaagagaat ttagcagtag cgagattggt ggatatggag gttatataaa cagcgaaaaa
    31201 atgtacttct aagagaaaaa tttatttttt ataaaaatag gaaagtatca ttatgaaaaa
    31261 gaccacagag ataaataaac ctaccacata aaaaat
//
LOCUS       DAZKMB010000019        30175 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000019 DAZKMB010000000
VERSION     DAZKMB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.019"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..1477)
                     /locus_tag="ACG8V3_000820"
     CDS             complement(167..1477)
                     /locus_tag="ACG8V3_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0299383.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1489..2847)
                     /locus_tag="ACG8V3_000821"
     CDS             complement(1489..2847)
                     /locus_tag="ACG8V3_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0299384.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3049..4617)
                     /locus_tag="ACG8V3_000822"
     CDS             complement(3049..4617)
                     /locus_tag="ACG8V3_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0299385.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEVMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4621..5031)
                     /locus_tag="ACG8V3_000823"
     CDS             complement(4621..5031)
                     /locus_tag="ACG8V3_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0299386.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5028..5951)
                     /locus_tag="ACG8V3_000824"
     CDS             complement(5028..5951)
                     /locus_tag="ACG8V3_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0299387.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5948..6826)
                     /locus_tag="ACG8V3_000825"
     CDS             complement(5948..6826)
                     /locus_tag="ACG8V3_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0299388.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6813..8150)
                     /locus_tag="ACG8V3_000826"
     CDS             complement(6813..8150)
                     /locus_tag="ACG8V3_000826"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0299389.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8153..9256)
                     /locus_tag="ACG8V3_000827"
     CDS             complement(8153..9256)
                     /locus_tag="ACG8V3_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0299390.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9330..10838)
                     /locus_tag="ACG8V3_000828"
     CDS             complement(9330..10838)
                     /locus_tag="ACG8V3_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0299391.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10804..11496)
                     /locus_tag="ACG8V3_000829"
     CDS             complement(10804..11496)
                     /locus_tag="ACG8V3_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0299392.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11518..12492)
                     /locus_tag="ACG8V3_000830"
     CDS             complement(11518..12492)
                     /locus_tag="ACG8V3_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0299393.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12792..13664
                     /locus_tag="ACG8V3_000831"
     CDS             12792..13664
                     /locus_tag="ACG8V3_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0299394.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14122..16320)
                     /locus_tag="ACG8V3_000832"
     CDS             complement(14122..16320)
                     /locus_tag="ACG8V3_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0299395.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16552..17544
                     /locus_tag="ACG8V3_000833"
     CDS             16552..17544
                     /locus_tag="ACG8V3_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0299396.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17604..19025
                     /locus_tag="ACG8V3_000834"
     CDS             17604..19025
                     /locus_tag="ACG8V3_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0299397.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19018..20517
                     /locus_tag="ACG8V3_000835"
     CDS             19018..20517
                     /locus_tag="ACG8V3_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0299398.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20556..21950
                     /gene="uxaC"
                     /locus_tag="ACG8V3_000836"
     CDS             20556..21950
                     /gene="uxaC"
                     /locus_tag="ACG8V3_000836"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0299399.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22109..22792
                     /locus_tag="ACG8V3_000837"
     CDS             22109..22792
                     /locus_tag="ACG8V3_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0299400.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22794..23630
                     /locus_tag="ACG8V3_000838"
     CDS             22794..23630
                     /locus_tag="ACG8V3_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0299401.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23641..25395
                     /locus_tag="ACG8V3_000839"
     CDS             23641..25395
                     /locus_tag="ACG8V3_000839"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299402.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25671..26012)
                     /locus_tag="ACG8V3_000840"
     CDS             complement(25671..26012)
                     /locus_tag="ACG8V3_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0299403.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26080..28251)
                     /locus_tag="ACG8V3_000841"
     CDS             complement(26080..28251)
                     /locus_tag="ACG8V3_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0299404.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28462..29319
                     /locus_tag="ACG8V3_000842"
     CDS             28462..29319
                     /locus_tag="ACG8V3_000842"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0299405.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29390..>30175)
                     /locus_tag="ACG8V3_000843"
     CDS             complement(29390..>30175)
                     /locus_tag="ACG8V3_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0299406.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9029 a         6225 c         5346 g         9575 t
ORIGIN      
        1 tctttttaac gacttagtta tcttatcacg tctaaaaaca tgtgtcaact cttttttgga
       61 attttcataa aatgtttcaa tgaagcgcga caacgttgtt taatatatca ttaaaaaaaa
      121 taaaggtcaa gctaaaatat tatttaagct tgaccttatt ataatttcaa cgattgtcaa
      181 aagtattgtt tgcagctgtg ttgccagttt ctatagataa atcacagctt ttgaaacata
      241 agttttgtac tgcttcgatt cgcccgaatt cttttgcagt tatttggcaa tgagaaaagc
      301 agacatcttc tataggcttt tcaggaaatg ctactaaatc aaaagctcgg gaaggaagtg
      361 agtaacctgg agaaaggcat gcctgtacgt ttttgatgtg tatgtctttg actttcgtca
      421 tttgctttga ttcaggtatt tcttcagcca ctgcttgcca actttctggc atttgagcaa
      481 ggtcattgct attttttcta ttgtattcat tatgccagtc cattatccaa gaaaaaggaa
      541 attgtacatc aatcatattc agattttcta ccctgatatt ttcgatcact ccccctcttt
      601 cttttgaaga tttcattcga ataccacagt ctgactgaaa aaaatcgata tcgtgaatat
      661 atacgtcact aactccagca ctaacttcgc taccaatagt tacaccgtat cctgaacgga
      721 ttttacatct agcaacctcg attcttgatg attttcgatt gactcttgca ccattgccat
      781 ctctgccaga cttaatagca atacaatcat caccacaggc caaatcacat tcatagacac
      841 gcacatttgt tgaagaatca atatcaatcc catctgtact tggtccatca ttgttgcgga
      901 tgattacttt ttctactaat acatcgttag aataagtgat ttgcaagttc caaaaacctg
      961 atttctgaaa aatcaaatca cgaacaacaa catgttctgc atgatacagt aaacaagcgc
     1021 gtggacgttt aatagcataa tccgcaatcc aacgaagtcc cttttgatcg tattcagctc
     1081 ttgtcccgct tttttgatct tttccccaat acagctccca ccaatggtct ccttgaccgt
     1141 cgattattcc ttttccttct ataagaatat ctttcgccga gagaacattc aaaatcgctg
     1201 ctggccattt catttctacg ccggctactc gtgtatcgat ttctggaaat gcttcgatat
     1261 ctgttctccc cttgaggact gcaccttctt caaagataag atgactctta tcttttaaaa
     1321 aaagggctcc gactaaataa atgccttttg gaataaatac aggtaagtgt tctttcgcac
     1381 cttgatcgat tgctttttgc agtattattg tatcatcttg ttgagtgtcg tttgttttaa
     1441 agttcaaagc attgataatc cctttttttg tccgcaatac gtttctccct atctttcatt
     1501 gatttttttc aattcttcac caataaatac agtttttgaa cgattcagtg ggtaacctac
     1561 tagcaaaatg attgcggata caaataagca gaatgctggt aacaagtttg ccatcgtata
     1621 aatcctttct tgtactaaaa ctgattggat ggctccacct gttgtagacg attggtaacc
     1681 aatgagtgcc agcataaatc cgcctaccgc tcctgcacat gcttgtccta cttttctcgc
     1741 aaaagaattt actccatata ctgttccatc ttctcttaag cctgtcttat attgatgata
     1801 atcaatcacg tcagtaatga atgcccagac catcaaattg aataaaccag atcctaaagt
     1861 agcgataaat aaaagaatca gatataccca tgcatttgtt atatgaagga aatacatcag
     1921 caagtacata catgaagaag caagtaacgc aaatacgctt gcttcctttt taccaaataa
     1981 ttttgtcagc tttgtagcaa atggtgctaa aactactact gttccgtaag taaataaaag
     2041 agcaacagac attgcttgtt tattatggaa gtaatcattg aataaataag tcatattcgt
     2101 cccagctaaa atctgattga tcacaatgaa aatgtcaaca atgactaaaa taatcagtgc
     2161 tcgattagct aacaaacctt ttaccaactg agaagcagat actttttcgt ttttgacaac
     2221 tcttactctt tcagtagtca atttacaagt taacatatat gcgacaaatg ctattgcagc
     2281 acatccgatt gaaatccaaa agaaatgatt ccctgaaacg acttgttgtc cactcttgtt
     2341 atgggtatac attaaaatcg gaatgaagaa tcctgtagtt gatccgccaa tcgcagaacc
     2401 aatgcttcgg aacgtggaca acgaggtgcg gtcattagga tttgagctaa ttgctgctgc
     2461 cattgctcca tacgggatat tgattgttga gagaaaaatc ccataaacaa tgtatgaaac
     2521 aaaaatataa gcaatcttca ccgtcattgg aagatgttgg acgaatggca aaaacaaaat
     2581 gacagtcact aagcaaaatg gatacttcat ttttctgatc caaggaataa atcggccagt
     2641 ttctgttaac tctgagacat cacataatct tccaaccatc acatcagcaa acgcatccaa
     2701 aattctcgaa atcaagaata acgtacctac gattgcccct gaaacgccaa gaacattcgt
     2761 ataataaatc atcaagaaac tattgactaa acctaaaata aaacagttac ctagatcacc
     2821 gaacatgtag cctatcttat cggaaatccc aaattccttt ttttcttgtt tttatattca
     2881 caatgttggc tttttattgc attatcttga cagataaaaa acaaaaaata atctaaaaac
     2941 attgatgcat aagattgata acttttttta cataacaata tatacaaaca aaaaaattca
     3001 ctaactagtg aaaataagtt agtgaacctc atttattaaa gaattaaatt acagatgatc
     3061 ttttccatca tacttgaaaa actcaaaatc agcataactt cctctttttt tatttagatc
     3121 atgcactgct atcccgataa aatttccagt aaaccctcct gagagtatac acatgttttt
     3181 ttgcggacct actttcttcc attcttcagt tgatacgtct ttcaaatgaa actgtgctgt
     3241 ctctttcttt acagtgactt tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc
     3301 ttctatttgt gctactccgt tttctgattc tatcatacgt aggcattttc ccttgtcctc
     3361 attccaagta acataacaat aaatataatt ggattcattt aagtacagta ccagtccagc
     3421 catctgatta aaattattag gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc
     3481 cgtttgccga atagctaaca aatgatgttc gaaggtagat tgaagagatt caccggaaat
     3541 cagacgtaaa aaacctggtc tacattttgt atcacaccaa tcagcatttg gcaaaatgcg
     3601 tctggcattc cagttcttct ttagctctcc ttcaaatgta tccataaact gatgatcttt
     3661 ttcttgttgt acctcttcat ttgtctcaat cattacttct tccatcggag ttgtctggct
     3721 atctttaagt cttaaccaat cgtcggacgt ccaatacact tcttgaatag cggtttctct
     3781 tcccaaaata gccgcaccgt ttaatggtct ggtacaaaga tatgtcatgt accatcttcc
     3841 tgacggggtt tgtactaagc tcccatgtcc tgtacattgc aatgtagaag caggtttgtc
     3901 actagctgtc agcataggaa aaccaggatc aagctcgtaa ggaccagtaa tattctttgc
     3961 tcggcaaact gtaactgaat gtcctcttcc tgtaccgcct tctgccgtca tcaaataata
     4021 atacccgtta cgaaaataca aatgtggtgc ctccgttttc gctaattctg tacctgcaaa
     4081 tattttatag atgggaccat caagcttctt cttctctgca tcatattctt gtaatacgat
     4141 accgctggat ttattcggtg tgtccagacg ataatcccat agttcattga caagccattt
     4201 ttttcccgtt tcaagatcat ggaacaaaga aggatcaaag ccgttactat ttaaataaat
     4261 tggttcggac caaggacctg tgatctcaga tgcagtgatc aaataattgt ggacatcttt
     4321 aaacggacgg cctgcacttt tgacatctgt gtaaacaaga taaaacaatc catcagcata
     4381 actgatctgt ggcgcccata tacttccatt tgtaggattt ccttgcaaag atacttgagt
     4441 ggtcaaaata tccgttgtat gtgaccagtt gactaaattt ttcgactcat atactcggat
     4501 tcctggaagc cattcaaaag aggagacaat aatgtaatac cgctctttca ctcttaatat
     4561 attaggatct ggattgaatc ctcttaaaat aggatttttc actttgatag tctgcatgat
     4621 tcaggctcct tttctcattc ttactcgata taaactgaat gccccagctg cgaaataaaa
     4681 aaatgcaaaa ggaaacgcaa taaactgagt gccaatcaac aatatgaatc ctgttataaa
     4741 atacaaacca gtggcagttg ttaagtattt tcctttagca gtcaaaaaca atgctactgc
     4801 taaaaaagca agtgaaataa acaacacaac ggcaaacaga ccagtcatgt cctgtaataa
     4861 tccaaatgta ttttctaaag acaaagttga atgatctaca gcaagcgact gataactttt
     4921 tttaaactct gacgcagaca tattacttaa agtaactgct aacccgccaa agaaaatcaa
     4981 agatagactg ccaccgacca ttcctagcaa tagttcccat tttcttttca tttctgaacc
     5041 ccctgctttt ctaaatgtat ccattcaggt cttttgatcg atgtttcata attgttatta
     5101 cgttcagaga atttgacagt ctgttcattt ttcttgtcat cccaattatc ccaaccagtt
     5161 gggtgaacat gcccacctat cttacacgct tcaaataaga catttgcata cggacgccac
     5221 ggtcttccta aatagacgtt tctagtatca ggttctgcag tcaaggtaca gctttcaaaa
     5281 cgataccctg ttttttgatt tttcggcgta gatgctgctg tgatgtatcc aggattttta
     5341 ctggctaagc tatggatatg acactgatga aaaacggcat ctgccccacc aaatatgaaa
     5401 tccaccgttc cttcaatcca gcaatattca taggtctgac gacaatactc aggattggca
     5461 gaagccggtg tacaaaaagg agtaccatct ttttgcaaat caggcaacgg acccgtgcat
     5521 aaggtatcct gataaccaga tagtcgacag ttttttaaag atacgcgatc tcctgaattg
     5581 aataaagcaa cagcctgtcc taccttttct cctggacctg ctgtattttc gatgtgaaga
     5641 ttttctaata cgacattcga tccttctaag aaaaaagtag cacttcggaa agtccctaac
     5701 tggctaccgt caagatactc ctgttttgca aaacgattac cggagataac tactgttcct
     5761 gctcccacaa gtttaaaatt ggataatctg ctttctatat attcatcata tcttccttct
     5821 tttactatca agacctttgg atggctatcc ggcaggcctg ccaaataatc gattccttct
     5881 tggatagttt caaaatccgt cggggtatct atagcattct ttccaattat cagtttgtct
     5941 ttcatcctca taaatgctcc cttatgaatt gtttccaaat atttctcatt tcttgtgttt
     6001 ccatatgacc gcctgttaaa gaaaaagaag aaaagttttc tggactaccc attgattgat
     6061 actttttgtc cagccctttt ctcagcttca gtacgccttc gtccaagcac atcgtatcgt
     6121 tttttccaac taagctaaga cgagggcgcg gagcgatttt ttcttggatt gctaacgttg
     6181 tataagctgt caatagattt ggcacatagt aataaaaccc atgatgatct aggcggcgat
     6241 gtagcaaaag tatttcgatg tccacctgtg ccgctagatc aatacaaact ttgacacgag
     6301 aatccaatgc tgtcacccac cagcttaata agcctcccat agaaagacca atcgttgcga
     6361 gccgttgagt atctgtgtct tcccttgtct ccaaataatc gatcaagctg atcacatcat
     6421 aaatacgcat tccccataac gtttttcctg ttaataaaaa ttgtttgaac aactcgcttt
     6481 cagaaatacc tccgcgttct tcaaatcccc aagcatcaat cgaccaaaca gcatatccca
     6541 ttcctgtcag tacttctgca aaagatggag aagctaagta aggagcacca tggatcaatt
     6601 cacttttccc gactgaaaaa tctccgccgt gagaatgcag gtaaaggacc ggcggaaacg
     6661 gtccttcttt ttctggataa gcatagaggg ctggtactgt ttccaattca tttaagtaca
     6721 attgatagta ttcaagcata aaaccgtttt gtttttctcg gtataatcgt tttccactag
     6781 gggtatgtct gctagatgga gcacctaaaa aatcattcaa ctgcataagc aaagttttta
     6841 cgaacaacca gttcatccat attttctgat tgatttgatt ggaatttctc aatactgatt
     6901 tctttagtat tttctaatag aaatgctggt ccctcgtgat tttcaatcga catctggtta
     6961 aaggaaacct tacccgcaaa gccaatataa aatccttgtc tgctcatttc ctctattcct
     7021 gtcatcatag caggaacacc gggaactgca tcttgggcca tcgagacatc gatattgtca
     7081 aatgtgattt cttgtatgaa ttgttccgct aatccataaa taaaacctgc agaagcatgg
     7141 acattccttg ccgtgatatt cgaaaaatgg atccttctga atgctggtgt ccgttcatct
     7201 atcggataag cctttttttc ccatacatac ggttctttcc ctcgcggacc acagaaataa
     7261 taaagattca gtataaatgg acacatcaca ttatccatga ctatattgga tactcgaata
     7321 tcttcaacga tcccgcctcg acctcgtcta gatttcaatc gtattcctcg atcagtttct
     7381 tggaagatac aatttgaaat ggtaatatta cgaatactac cactcatttc acttcctaat
     7441 acgacaccgc catgtccatg gaccatcgtg caatttgtga tcgtgatatt ttcgcaagcg
     7501 atcctttcgt acgtatcttc agtccctgcc ttgattgcaa tacagtcatc tcccacgtca
     7561 atatggcaat tactaatacg cacatttttg catgattctg gatcaattcc gtctgtattt
     7621 ggtgaatccg ctgggttcaa gatcgtcaag ttatcaaacg ttgcattact gcaaagaatc
     7681 gggttgatcg tccaacttgg tgactggatc aatttaatat ctttcacagt aattcgatga
     7741 cagttatgga aactcatcaa tttaggacga ggataagcga gattatctgg ttcatttcta
     7801 aaagtatgcc accatttttt tccatttcca tctaatgtac cgaaaccagt gacagagatg
     7861 ttctctacat tttgagcata gatacaggaa gcataaactt ttcgatggac tccttcccaa
     7921 cgtgaatgaa caacaggata atcttttgga tcgtcagaga atttcagaac cgctcctgcg
     7981 cttaaatgaa gttcgacatt gctttttaaa aataaagctc ctgttagaaa ttcacctgct
     8041 ggaacaacta cggtcccgcc tccgtcactt gctgcagcat caatggcttg ttgtattgct
     8101 tcagtattca attcatcaat tgatgcgcca aatgtcaaaa tatcatacat atttataatg
     8161 cctccacttc aatcaatgct tgcaagaatg caccaacgcc tttttgatca ttacaaataa
     8221 ttggttcact gatgtagtaa gcatatgaac catctcgctg atctgctccg cctaaccctg
     8281 ctacttgaca gtttttattc aagttcagcc agccttcttt tgtttccaga acaaattcat
     8341 caagtaatcc ttgatgtgat ttcttcactt gtggaagcca ttcttctttg ttcagcagtc
     8401 ccaaacgtat tcctttagcc attgcgtaaa caaacatact actgccggaa gcttccagat
     8461 aatttccttt ccgttttgtt tctgttgtta cttgatacca tgtcccgctg ttttcttctt
     8521 gatatggaag taatgcagcc agtgtctttt ctagaatttc tttcagcaaa gaacaatctg
     8581 tttcttcatg aagtaattca taggtatctg ctgcagccat caaataccag ccaatcgatc
     8641 ggctccaaaa atgatgggat agacctgttt ttggatcagc ccatggttgg accttctttt
     8701 catcccatgc atgaatcagc aaatttgttt ttggatcgaa tagatgttta taactgattt
     8761 caaactgtct caaaacatct tggtaatctt tttcttcact aaaattctgg ataaattcag
     8821 cataaaaagg agaacccata tataagccat ccaaccaaat ttgataagga taaatttctt
     8881 tatgccaaaa agctccttcg gatgtgcgtg gatggtttgc caactgctgg gaaaggagtt
     8941 ctgctgcttt tttatatttt tcttcgcctg tttctttgta caataaaaag aaaagctttc
     9001 ctgtatttac atggtcgata ttgtattctt caacgctgta gccacggatc gttccatctt
     9061 cttgcacgaa atagtcgata ttgtctttta tgaaatcaaa ataaattttt tcttgggttc
     9121 gttcccaaag taattggaac cctttaagta cgacgccata gtcataagac cattttcctt
     9181 tataccactt ttcttcatat aagcgaggtg ttctttccat gatacttttt gctaatttct
     9241 ttgaccagtt ttccattatt tgctctcctt taaaaattaa aataggattt gggacaaata
     9301 ccaagaagta tttgtcccaa tcgtctatat tactgtgccg ctgctttgta tgcttcctcg
     9361 tactcttgtg tgattcgatc gcctccttgt gctttccagt cttctacagc ttttttgaat
     9421 ccatcttcgt caagctgtcc catgatgtat tgcaccgtag catcaatgac gattttttca
     9481 atttctgatc ctacttcatt gttcgtcggt gaatccaatg gcacagaagg atccattaca
     9541 gcaaattctt cattttcttt gatcaattga ttagagaatt ctttttcagg gtctgtatct
     9601 ttgaaagaga acgtcacttc actaggacga gagcttgata acggttgcac ctcttgttgc
     9661 caaagatcca tatcgataaa ttcgatagaa ccatcatcat tcaattcata atgcgttcct
     9721 tcgataccgc cagtcatcaa tttatacaca tcttcttcta aaagatcatc aacaaattga
     9781 agtaatttct tcaattgtgc ttcgtctttt acttgagatt tgggaaatgc tagcaagccg
     9841 ccaataccat ttccttctga ccaaacatga taaccgccgc cttcaccatc tgagatcttg
     9901 tttacaggca ctaattccat atcatcttga ataccttgac ccatcgtctt cagattcttg
     9961 atatccacca ttccagtgta gattccagtt cttccttgtg caaattgttc ttgctgatca
    10021 gtcttttggg taactgcaaa gtcttgtgcc aaatagccat tctcaaatag atctcttgaa
    10081 aaattcaaag ctttcatata ctcttctgta tcaaattctg gagtgaattt gccattatcg
    10141 tctacagccc agccatttgg tgctccaaaa taagaagtga aaagtttaaa gctggtataa
    10201 cgaatatcac taggcgcacg atcgccaaaa ccaactgtgt cgtccacacc atttccatca
    10261 ggatcgtttt ctgtaaattg tttagctact tcataaagct catccatggt agttggaaca
    10321 tctagtccta gattatccaa ccagtctttt cgaatgacta agccagcacg agcgtagtct
    10381 ttttgtatcg gtacgccgta caaggcaccg tcgatactcg cagcttcgat tctatcttca
    10441 gaaatttgac ttaaattttt atagtctttt aaatattctg atacatccca gaatacgcct
    10501 gattttaacg cattgcggac agaagagttt ttcaacatcg caatttgaag tgtaacgata
    10561 tctcctagtt catcagaagc caatgcagtt gttaatcttt cttcttttga agcatcagga
    10621 atccagttga atgtcagttc cgtatcggtt ttttcttcga tcaagttcac aatcgtgtct
    10681 gtaggcggtg aagcatgatg caaaatattt aaccaagtga ttttagcttt tccatcttct
    10741 gtctctgaag aactgttact tgaactgctg ccgcatgcgg caagtgctag aattcctgca
    10801 aaactaagac ctaattttac gagcgttgct tttttcataa attttctcct tcactgtttt
    10861 aataatattt ttgttttctt ctgttttttc tgtatatgag ttcttccatt ccaaatggca
    10921 aaatatagaa tttgctgtcc acgtgactgc ggaaaagaag agactgccac cgataaaggt
    10981 taaaacacct ggcattgttt ggatgatcat cagataaatc cctaccatac ttaatatcaa
    11041 cactaatgag taatgaagat gtgagaatcc gaataaaaat gccattttca ttttgaaaaa
    11101 atatcctttc caatcataat gagccatgat tgggaaaata tagagtgctg agagcacata
    11161 tatgaacgca gcaatataca aagcgatcct aagataccaa ttgtgtatga aagaaagatc
    11221 cacaagtaaa acaatcccga tggcgccata tatccagctc atcagcaagg tttcacgata
    11281 gttttcttta aaatatgtga agtacgtttt cgtcaaagga aacgtttctt ttgagcgcag
    11341 ccattttcta atcactgcaa ccatcgcata cgatgcagga ccgaaagtaa aaactcccaa
    11401 acctgcgatg actaaaccgc cccacagaaa attcaaataa accaactgta aaatacgatt
    11461 tagccttccg tttattttat ctaatgactg cagcatatct ttccctctct ttctatctta
    11521 gtaaacgccg tcttctccta gctttttggc aatcttatta gctaaaatta ccataaacaa
    11581 tcctacgacc cctttaaaca atcccattgc tgtactgaaa cttaactgtc cattcttcaa
    11641 tccagctgta taaatatagg tgtcgaaaat ttctgcaacg cttcgattca atgaattcaa
    11701 taacaagtac atatgctcga accctaagtc taatgtctga ccgattttta gaatcagcaa
    11761 ggtaatgatt actggacgaa ttgccggcaa tgtaacatgc catgttttgc gtaagcgtcc
    11821 tgctccatcc atttctgcag cttcatacaa ctgtgtatcg actgcagtta tcgctgaaag
    11881 gtagatgatc gtagaccagc ccaactcttt ccagatcact tgtccgatat atactgtgcg
    11941 tgtccattca ggggatgtta gaaaactgat tttttcaaat cccattgttg caaggaattc
    12001 attcaatacc cctccgtcaa cattcaaaaa tacataagta atcgaaacaa tgattaccca
    12061 agacatgaag tgagggatgt aaatgatcgt ttgaatcccg tttttcaatc gtttgttcgt
    12121 tacttcattt aatagtaaag acaaaatgat tggcatcgga aaaaacacga agatatttaa
    12181 tccgaacaag atcaacgtat ttcttaataa tgtaaagaat gtcggttctg taaacaaacg
    12241 aatgaaatgt ttgaaaccga cccaaggact tcccattatt cctaaaaatg gttgataatc
    12301 ttgaaaagca ataacgagtc cccccatagg aagataccta aaaatcaaga aataaaggac
    12361 tcccggcaga atcatcacat ataaaaattt attcgttttg attcctgcaa ttattttttt
    12421 tcgtcttctg agcttttctt cttttttcga tagcgtcagt tgaaatggtt cttgtttctt
    12481 tacactttcc ataagcccct ccttagtttt gtgaatgttt gctgcttatg acttcatgat
    12541 agataactat ctgaattacg tatataatca tttttaccgt aagtaaaaac gcttacatag
    12601 caatgatttt tgtgctatac aaaacgataa agtgtatttt ttcacaaata aactacgcat
    12661 agcaaacctt ctattttttt ctatctgaca cttagcatag gaaatgatta tatacgtaat
    12721 ctttcaccga tgataaattg aacatataga aacagaaaca tttaatttat tttgaggagg
    12781 agcctatcta tatgaaaaag aaagtctcta taggagaaag aagttttaca cttttcaaca
    12841 atacgtttct aattttgcta gctttgatct gtatcgttcc ttttttaaat atcatcgcta
    12901 catcttttgc ttctacacaa gaagtcgtcg cgaaaaagtt tatccttttt cctactacct
    12961 tttctttaga cgcgtatcgc tacattcttt ctacgccgac catttttaga gcacttgctg
    13021 tttcgattgg tgttacaggt gtcggaacga tcgtcagtat gtgtgcaact tcactgatgg
    13081 cctacggtct ttccagaaag tatttgtttg gtcgaggttt cgtcaacttt cttgttgtat
    13141 tttctatgtt gttcagcggg gggatgattc caacgttctt agttgttcgt tctctagggc
    13201 tggtcaattc ttactgggcc atgatcctgc ctgtggctgt taatgcaatg aatatgatca
    13261 ttatgagaaa ctttttccaa gccttgccgg atagtttgga agaatcagca aaaatggatg
    13321 gttgcacaga ttttggtgta tttttcaaaa ttatgctgcc attagcgtta ccgtctattg
    13381 ctactatctc gcttttttat gctgttactt actggaacac ttatatgact gctattttgt
    13441 atatcaacga ttcatctaaa tggccgatac agattctttt acgccaaatc gtcattgtat
    13501 caagtgggat gcaagcagaa agtagtgccg ttgatgtgat tccaccagca caaacagtta
    13561 aaatggctgt tatcgttatt gcaacagtgc ctatgctgat tgtttatcct tttgttcaga
    13621 aatattttgt caaaggagca ctcgtaggtt ctgtcaaagg gtgattatgt actgaacgca
    13681 aaatggtagt tgtgtcagaa gtggtcagct tcaagaaata agaaggcatt tacgaaaatt
    13741 gcttcttaaa tttttgtgaa atttcggctt atttttcgaa gaatctactc ctggaacacc
    13801 gtgtatcaga ggttgtgaaa gagacgttca gccttgagcg atgataaaaa ctctcacaaa
    13861 cattgcgacg agtaacccac aggagcaata agaagcgatt ttgaaaaata gcttcccata
    13921 tttcacaaaa ttatgcctta ttgtcgatag cctgtctctt gaacgccatt tattcgtaaa
    13981 aaagaactgt gacaagactt ttgtcacagt tctttttgcg tgcttagcct tgaaatttaa
    14041 ctatccataa atacaaatac tagggttatt ctaacttctt ttattgcttg aatcagtcca
    14101 caagaaaaac agcctcttga ttcacaaata tttttttcga tattctcccg gcgtaatcga
    14161 ttccttcttt ttaaagaaac gaataaaatt ttgcggattt cgatattgca atctttctgc
    14221 aatctctttt acggagaggt tagtctcttt caaccatttt tttgctacct ctaaacgatg
    14281 attttgaaca aaatcaccaa atttttctcc tgtttccttt ttaaatacat tacttaaata
    14341 gttagggtta taatgcaacc tatcagcgat cacatctaaa gacagatcac gatcatattc
    14401 tgtgtagacg atatgcataa tattctccga caaattttta aattcatgat cagtgattgc
    14461 ttgagagcat tctgtgattg gcagaactaa ttgttctaaa acaagttttt ctagttcttt
    14521 gggatgataa ttgttcagtg cttttacata aatttgtttc atgttatcaa agttttttga
    14581 atctactccc aataactgtc ctaattgaac gagttcattg atcaagcgga ccgaaactac
    14641 ttcccgactc aaaggatttt tattttgtgc aaacaatgca tcaatcaatt catgtactaa
    14701 attcttcact tcctgatttc cagaacgaat ggcttcaaac aatcgatttt gttcttctat
    14761 cggatatcta ataagtgttt tttcatgagc agcaggaacg atttcctgat aaaaaataat
    14821 tgaacttgga ccagtattta cacgataata cagggcttct tttgcccgat caaccgattg
    14881 cttgctttca ttcagcgttt cgaaccggtt actgatgcct atactgatag ttaaattcaa
    14941 gtattctttt acacgttttt gaatgagtcg gcaatactcc atgacttttt tgcttgcttc
    15001 tgcatcatct ttatccattc gataaatagt ggcttgcatt tcttcattca aaacgatcgg
    15061 cagcattcga ttttcctttg gcacaatttc cgtgatcatg ttgttgatcg ctaaaagtag
    15121 caaatcacgc tccccaccgt gactatcttc taacagatcg atttgaacca aagctgtata
    15181 atagcagtca ttttgagaac tataaccaaa ttgctgcaac cgctgatcta gttctcgcct
    15241 ttctactcga tttctaaata aactaagtac aaatagcgtt tccagctgtg gcttttgggt
    15301 aactaagtgt gcacttaacg cttctttttc tcctatgatc cgactgactg attgagcgac
    15361 caaatctagt tctttcccac tgaatccttt tgatttaaga ttcagacgtt cctgtatttg
    15421 agaaatcgga cgggcaaatc tgtccgaaaa taagtaggaa agaagaccaa tgagtcctac
    15481 cagtgttaaa gacactgcaa ctagcccgat tctcagattg cggatcattg cgccaattgc
    15541 actagagtca acttctaatc tgtaaatcca attattataa tctgatttca gtactatata
    15601 cgttttatct ttttctctta tcactttcag ctgattgtaa tctagatcca cgtccgttaa
    15661 tacagttggt tgctctttat tttttactgt ctgagtagta aaaaggttgc caacttcgtt
    15721 ttcaatagaa agtatgtctt ctccactttc accaacaact tgttctattg tatgtttctc
    15781 tatcgtagca attcccaatg catatttttc tttttcaaac attggcaaag aaatcaccat
    15841 ttcgattcct ttgtttttct tttgccaaaa taaattattt ttgttattta tgtacttctg
    15901 ttgatacttt tcaatttcct cttcggtcag ttgactcaac gatccattga ttactcccca
    15961 tttaccagcc aaactgacta aatcataatt attatcatcc ataacaatcg tttctataaa
    16021 atttagttct ttcctgattt cagaataagt ggagtaatct gtatatgtca gcggatgctt
    16081 gaatatgtcc gagaaatttt tggtcgaacc atacgtagaa aacgaatatt caatcgttct
    16141 aattttccat tcaacgttag ttttaacttg cctgagcaaa ttctttttgt ctgttaaata
    16201 actcgattcg atcgattcat aggtattctg gtaaatgaat aaactaaatc ctatgacaag
    16261 tacggctcct aataaaaaca tcggtaaaaa tattttataa aatactgtac ttttcttcat
    16321 tttcttcctc tcctcctttt aaaataaaag gctttcattt ttctgtaaaa ttgatactca
    16381 tctaattaaa tgagcattcc ccgctttcaa gaatagcata attaaaaata aaaatccaag
    16441 taatttttaa cgtgtgcaat cttttcatat caaagatcta ttgacttatc gtagttatcg
    16501 taatatgatg aatgcgttaa caaaaagtga aaaagtaagg aaaatgatgg aatggtaacg
    16561 atcaaagatg tcgctaaagc agctggcgtt tctcatacga cagtttctcg tgctttgaat
    16621 aacagcagtt taataaaaaa tgaaacaaaa caaaaaatac gacaactagc taaggaaatg
    16681 aattattcgc caaactatag tgcaaaaggt ttagtgaatc aaaaaaaata tctaatcgga
    16741 ctatttttct caagtttgca taaaggaact tcatcaaatt tcctagcaga ttccattgca
    16801 ggcatccatt cagtattaga cacaaattac agtttatctg ttgaaagtat cgatgttgtt
    16861 gatttaacag aaatcaacct gcaacgctac gatggactga tcgttatgag tcaaagtgat
    16921 gatgatcagc ctttcatcga ctatctcaaa gaaaaagaat ttcctttcgt tgttgtcaat
    16981 cgtcacatca atgatccagc ggttatcaac gtcactgccg atgatgcggc cggtgttatg
    17041 caggcagtag attacggtat tggatgcgga catacgaaga tcgcttatat tggcggaaag
    17101 aaaaattttc gttcgacaaa agaacgggaa aaaggggtat tgaaaagtct aaaaaaagcg
    17161 agcatccctg tcaatccagc ctatttttta acaggagatt acagcttaga aagcggcctc
    17221 caaaacatgg aatacctatt atcactttct gacctgccta ctctggtttt ttgcggcaac
    17281 gacgacatag cgattggtgc attgagagct gtacatttag ctggcctccg tgttccccag
    17341 aacatttctc tgatcgggtt cgatgattct ccagtcgtct cctatctcaa tccgccgctt
    17401 accaccgtac gtaagccttt gaaagaaatg tgtcaagaag gaacaaagct tttactagat
    17461 ttgatcgaag gcaggacgat cgaacaatct aaaattgagc tctccacctt gttgatcctt
    17521 cgtgagtcga tcaattactt gtaaactaca agtaatttta tataaaaatg ttaacgtgtg
    17581 cataacacag aaaggaagtt tatatgaaaa aactgacaaa agaattagtg aaaaaaaacg
    17641 aagcacctgt aaaagttctt cagtttggtg aaggcaattt tatgcgtgga tttattgatt
    17701 ggcagattca gcagctgaat aaccaacaat tgtttaaagg taatgttgca atcattcaac
    17761 ctttagaaca aggtcttgga aatatgatga accagcaaga taatttatat acggtcattt
    17821 tacaaggact cagcaatggc gaaatcgttg atacttccga gattatcacc gtagtagaac
    17881 aaatgatcaa tccttatgat gaatgggatg cttatctatc tctagcagaa aatgacgact
    17941 tggcctatat cgtctccaat acaacagagg caggaatcca attcgtggag actgataatt
    18001 taacagatac tcccccacag agcctcccgg gcaaactgac agccttttta taccgacgtt
    18061 tcaaattaaa caaagctggc tttaccatca ttccttgcga actgattgac agaaacggcg
    18121 aacgtctaaa agaaatcatt ttacgctacg cagaaaaatg ggatttagaa cctgcttttg
    18181 ttgagtggtt agaaaaagaa aatgttttct gttgtagttt agttgatcgc atcgtcccag
    18241 gttatcctcg cgaagatgca ggtgttttca atcaacggca taactatgaa gatcaattga
    18301 tggtcaaagc agaaccattc atgctttggg tgatcgaagg gcctaaagaa ttggagaatc
    18361 aactaccatt caaacaggct ggcttgaatg tcatcatcac agatgatatg actccttatc
    18421 gtgaacgtaa ggtgcatttg cttaatggac cgcatacagc gatggtccct cttgctttgt
    18481 tggcggaatt ggaaactgta gaagaagtaa tgaaagatcc tttattctcc gcatatgttg
    18541 atcagttatt caatctagaa ttgattccta tgctttcttt acctaaagat gaattagcca
    18601 tttatgctga ccaaatcaaa gaacgtttct tgaatccttt tgcccatcac aaattagaag
    18661 cgatttcatt aaatagtgtt tcaaagttca gcacccgtct gcttccagtg ttcaagaagt
    18721 atatcgaaga acaaaatcag gtaccaccgt taatcactgt ttcactagct gcgctacttc
    18781 ttatgtatcg aggcgatcaa gtaaagccac atgatgatga aaagacgata agcgaattta
    18841 ctgatgcttg gtctgataat ggaacagcta ttcctcgatt attgcaaaac gctgcgcttt
    18901 ggggagaaga tttgtctcaa ataccgaatg tgacagatac agttcaagaa atagctgcaa
    18961 agatcgatca aaaaggtgtc cggtcagtat tacaagaaat gaaaggaacg atggtccatg
    19021 cttgaaaccc tgcaaccaac aatgaaatta aatcgacgag acagtgtggt cgtcgcatta
    19081 acccctattc caaaacaaac acaactgacg attgatcagc aaacgatcac cactcttgaa
    19141 gatatcccgc aaggtcataa gattgcattg ggtgatttga aagctggaga caacgtgatc
    19201 aaatatggct atccaatcgg tcatgtgacg acagacgtaa ctgccggtca atggctgcac
    19261 actcataatg taaaaaccaa tctttctggt gaattggatt atcgatatga aaaagatgtc
    19321 catccaagtc attacctttt tgagaaccga acatttcaag gttacttgcg aaaaaatgga
    19381 aaagtaggga ttcgtaatga tttgttttta gtacctaccg ttgggtgtgt gaatggcatt
    19441 gctgaactga tcgtcaaaca gttcaaggaa aaacatccag atctgggaca gtttgaccat
    19501 attacgattt taaagcatcc ttatggctgt tcgcagcttg gaaaagatca tcaaaatact
    19561 cgagaaattt tagcggacgc agtaaatcat cccaatgccg gaggagtttt agtatttgga
    19621 ttaggttgtg agaacaatac agttcctgaa ttcaaaaaaa ttttaggtga atatgatgaa
    19681 gaacgagtga aatttctagt ggctcaagat gtttacgatg agatcgaaca aggtgtggct
    19741 ctattagaag agcttttaac agcggcggaa aatgaccagc gcatatcagt cccgctctct
    19801 aagttgaatg tcggtttgaa atgcggcggt tctgatggtc tatcgggaat aactgctaat
    19861 cctttacttg gtgcattttc tgactacttg attgctcaag gaggcagcac gatcttaaca
    19921 gaagtgccag aaatgtttgg cgcagaacaa gtattgatgg cacgagcgga aaatgaagaa
    19981 gtttttgact caatcgttga cttgatcaat gacttcaaac agtatttctt gtcttacggg
    20041 gaaccagttt acgaaaaccc atctcctggt aataaagcag gtgggatcac tacattagaa
    20101 gataagtctt taggctgcac gcaaaaatca ggaagctctc ctgtcgttga tgtgctacaa
    20161 tatggcgaaa aaatcagaaa accagggctt agtttattgc aagctcccgg gaatgatctt
    20221 gtagctgcct ctgctcttgc ttcatccgat tgccagttgg tcttgtttac cacaggaaga
    20281 gggactccgt ttggctcata tgtcccaacg gtcaaggtat ctacgaatac aactcttttt
    20341 gaacgcaaag gtcattggat ggattttaat gcaggcgtct tattagaaca accaatggaa
    20401 gtaatcttag aatcgtttgt tcaaaaaatc atcgctgtag ctagtggaga agaaacgaaa
    20461 aatgaacaaa atgacgttcg agaaattgcc atttttaaaa acggagtaac actttgattt
    20521 tcataaaaca gtataaaaag aaagaaggaa gatttatgtt tttatctgat gattttttat
    20581 taaaagacga ttgggcgaaa aagctttacc attcctacgc aaaaaacatg cccattattg
    20641 attatcactg tcacttagat ccaaaagaaa tttacgaaaa caacaatttc agcaatctga
    20701 cagaagcttg gttaggaggc gatcattaca aatggcgctt gatgcgagct tgcggcgtgc
    20761 ctgaagaaaa aatcacagga aatgcttctg attttgaaaa atttttagct tggtgtcaaa
    20821 ccgtaccaaa aattatcgga aatccgcttt acagttggtc acatcttgaa ttgaagcgtt
    20881 tcttcggtat tgacttgttg ataaatgaag aaaatgcaga gaaaatttgg acacaagcaa
    20941 atgaaatttt ggcagctcct gattttcgcc gtcgggaaat cgcaaaaaaa tcgaatgtag
    21001 aagtgatttg tacaacagat gatcccgcag acgacttgca ctatcatcag ttgcttgctc
    21061 atgaagaacc agatttgaag gttctacctt cattccgtcc agataaagca ctcaatattg
    21121 aaaaagaagg atttaatgca tgggtcacac tactggaaga agcagctgac caaacgatca
    21181 ccacctatca agaactgata gaagtcttag ggcagcggat agaatacttc catcaacatg
    21241 gatgtcgtgt ttccgaccac gctttagaca tcttacgcta taaagaagcg gatgaaacag
    21301 tattagaaga gatttttcaa aaagcacgag ctaacaaact attatcttca gatgaaatcg
    21361 atgcttatcg tacagaaaca ctgatacatt tgacccactt ctatcattct cataattgga
    21421 cgatgcagct gcacatccat gcttatcgta actgtaatac acaaatgttg gaaaaattag
    21481 ggcccgatac cggctatgat ggaatgaatg atctttcttt gactattcct ctgcaaaaat
    21541 tattgaaccg tgcagaagaa acggatcagt tgccaaagac gatcctttat tcattgaatc
    21601 cgaatgacta tcctgcgatt ttagctttga tgggctgttt ccaaaaagaa cagaatggaa
    21661 aactgcagtt aggttctgga tggtggtaca acgatactcg agctggtatg cgcgatcaat
    21721 tgacacaatt tgctgatggt agtgctttgg ggaattttgt cggcatgctg acagattcaa
    21781 gaagtttcct gtcctacact agacatgaat atttccgtcg tgttctttgt gaatttttag
    21841 gcgaaatggt catgcgagga gaagcacctg aagatgaaca acttttagga agtctagttc
    21901 aagcgattag ttatacaaat gctaaaaatt actttggctt ttttgcctaa ttgattaata
    21961 gtaacagtaa cgaatcaaaa acaggatagt gaaataacgg aatccgttta tccacgtttt
    22021 agagaagaag acggaccaaa tttgctccgt cttcttctct tgctgttctg cttcattgcc
    22081 tataaacctt tatgaaagaa gagataacat gcctactatc catattgccg gtgattctac
    22141 tgcggcagaa aaagatgaaa acaagcgtcc cgagacaggt tggggagaaa aaatctctgc
    22201 ttatttttca cccgagatcc agatcaataa ccaagcaaag aatggacgca gcaccaaatc
    22261 atttatccag gaaggaaggt tagcccgtct agcttcacag tttcagccga atgatttctt
    22321 atttgtacaa tttggacata acgaccagaa aatccaagaa gaaagaggta cgcttccctt
    22381 cggtagctat ttaaaaaatc tgcagatatt ttttgaatgc gcaaaaaaag cgaacgtcca
    22441 aatggtctta ctgacatctg tgacgcgacg agattactta gaaaacggaa cattgaatcc
    22501 agatatactt ggagactatc caaaagcgat gcgtgcattt gctgagaaac accatattcc
    22561 tcttttagat gtgttctcaa gatcacaaga attgttccag accttctcaa aagaagagac
    22621 aaaaaaattc tacctgcatc ttatacctga tacttgtaaa aattacccag aaggactaaa
    22681 agacaatacc cactttagtc cagaaggcgc tgataaagtt gcccgaataa ttgttgaatt
    22741 gataaaagaa tcccgtctgc ctcttgctaa ttatttacaa aaaggagttt gagatgtttc
    22801 cattaaattt aggcatccgt gcccacgatt tagatgttcg aaatcgtgaa gaaatcgttc
    22861 aaaaagtatc acagcttcac ttgtcccata tccaatttgc accacaaaaa gcatttcctg
    22921 aacaacttaa attaactgac atgacacttg ggagtgctgc ctattttggt gaatacttcc
    22981 aaaaaaacaa tatcgaactt tccattttag gatgctatat caacctatcc agcaaaaatc
    23041 aagaagttcg tgcacaagca ttggcaactt tcaaacagca aattgctctg tgctctgcct
    23101 atcaagccaa gatgattgct acggaaaccg gtagtgtctc tgtcggttac acaaaagaaa
    23161 attttactga agaagcttat cagatcgcca gaaattctat cattgaatta gtcgctcatg
    23221 cagaaaattt tggcgtaact gttgctgttg aagcagggat caatcatcca atctataact
    23281 atcagttagc taaacgttta atcgatgaag tagcttcacc aaatttaaaa atcattttag
    23341 attgtgcgaa tttgatgcat aaagaaaatc atgctgatca ggaaaaagtg atccgaggtg
    23401 ccttggacaa tttacatggt tatattacag cgcttcatct caaagactat atcatggaaa
    23461 atggaaagat ccgcatggta cctgttggca agggctgttt agatttccgc ccggtactgc
    23521 attatatcaa atatgagcgt cctctaatgt acgccactct tgaagctact cctgagattt
    23581 atgttcctga agcaatagaa cacttgcagt ttatttacca agattgttaa ggagaagatt
    23641 atggaggaac gaatacagaa aagttatcaa aaatatcatg caaatgcttt tcagacattg
    23701 ctgcatcttt atcagggaaa ttattggcgt tttgccatgt caggactctt ttttattatc
    23761 aaacatcttc catcttggtt aatgccgatc gctattgcca atgtaatcaa tgcagcttca
    23821 tcgaaagatt cttctcagtt gcaggccatt tatttcaacg cttttttgat ggcttgtctg
    23881 attggtcaaa atatattgac gaattatttt catgtaaaat accatagcac ttcgatccgt
    23941 caagtagaag ctggactaag agcgaccctt atcaagaaaa tccaagaact atctattcct
    24001 tatcaaaaag aattgcaatc tggacgaatc cagtcaaaga tcatccgtga tgtagaagct
    24061 atccaaacgt tgtcctctca agtatttgtc agcggattaa atatcgctgt caatttattc
    24121 gtagctttag gtatcacgct ttataaaagt cctgttgttt tcatgttttt cttattgact
    24181 gttcctctcg ccgcattcgt tactctcttt ttccgtaaaa ggatcagaca gtccaactca
    24241 gacttcaggc aagaaatgga agagacttct gcaaaagtga tcgaagtgat cgaattagca
    24301 ccgattgctc gagctcatgg gttagaaaaa cacgaaacaa ctcgcttaaa taagcatttt
    24361 actcaaatct atgaaaaagg attccactta gatatcctgc aatctgtttt cggatcagtc
    24421 agctgggcta gttttcagat atttcagctg atttgtttga ttttcactgg gatattagct
    24481 ttacaagggc gcatccaacc cggcgatgtc gtgatgtatc agacgtattt cacgacgatc
    24541 gtcaattccg tttctggaat gatcacgctg attcctacga tttcaaaagg catggaatct
    24601 atcacttcta ttggcgaact gttgattgct ggcgatgtag aaacttatca cggtaagaaa
    24661 aaaatccaac atttggatgg aaattttcgt tttgaggatg tttcttttct ttatccggac
    24721 tcactcaaac cgattatcga tcatttttct ctagaggtga aagctggaga aacaattgct
    24781 tttgttgggc catctggctc tggaaaatct acactcctta acttattgat tggatttatc
    24841 cagccgactt caggaaaaat ttttctagat gatcagcctt tggatgaact ggatatgcga
    24901 agtgtgcgaa attatttagc cgttgttcct caaaccactt tgttgttctc cgcttcgatc
    24961 aaagagaaca tcacttatgg cttgaaaaac gtatcaaaag aacgtttaga tgaagtcatc
    25021 gaagctgcgc aactttcttc actgattgac cagcttcctg atggtctgga taccttagta
    25081 ggcgaacatg gcaacaagct gtctggtggc caaaagcaac gtatctccat tgcacgagcg
    25141 ttgatacgtc agccaaaaat cattttgcta gatgaagcaa cctcagcttt agataaccag
    25201 tcagagaaaa aaatccagca agccctgaat tacctgacag aaacacccac tacttttatt
    25261 gtggctcatc ggctttcaac gatcaaagag gcagataaaa tcgttgtgat cgatgaagga
    25321 aaaatcgttg aaataggtac ttacgaaaaa ctgttagctc aaaaaggcta tttctaccgc
    25381 ttgaaaaatg aatgacaaaa aataggagct atgacaagac ttttgtcgta gctcctattt
    25441 ctgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca
    25501 tgagaagctg ttttttgaaa ttgttccttg taactcagag ttaaacggta ggctcacgac
    25561 ctcttggtaa atggtgttca agagacagcc tatcggcaat aagaagcaat tttgtcatcg
    25621 ctcaaggccg aacgtctctt tcacaccctc tgttgtttca taaaatagtt ttattcgctg
    25681 taaaggtcgc gaactggtgt catgatgata cggttcaagt cattgatgat tgtactgaag
    25741 ccttgctctt tttgcatcaa gtcattgatt acttcttctg attggatttt cacagccatt
    25801 tcttgtgctt tcattgcatc ttcgtcgctg aactcttgtc cactcatttg tttttcttgt
    25861 aattcgattt gcatcgcttg gaagtctttg aacagtttgt gtgcttcttc gtttgcttgt
    25921 actttctcgt acgcttcttt caacgctttg aattctggta attcacggat ctcacgttcg
    25981 atttgattcg cgctatcgta gatgtttgac atgttatgcc ctcttttctt ttagtagtta
    26041 ctctctattg taacatgggg ttattcgttt tcaaaggggc tactctcctt gatatttccg
    26101 ccagaggtct ttagccgctt caccaattcc ctgaacgcct tctttcacca tatcgccgaa
    26161 gtttttcagc ccttcttttg cttgttcacc atattgattc aagttatctt gccaatttcc
    26221 agactctcca ttagaatttc ctgtgtttcc aacttgatct gcagctacca cttgtcctcc
    26281 tgtggcatag gcatctgcta cactaaatcc tgtttttgaa gtatacggaa ggatgccttc
    26341 tgccgctgat ttgaagactt tcccgacaac atttccactt gtcccttcca aatagtgcag
    26401 cttgctggat tcttcgtaac ccatccatgt cgaaatcact acgtctggcg tgtaaccgat
    26461 catccattgg tcatttgctt ttgtggcatc gaaattagtt tctgtggttc ctgttttccc
    26521 tgctatcgta taacctgcag gattcgcagc agccgctgtt ccatttgaaa atgtaccaag
    26581 aagcatgctg gtcatttcat cggctgtttc ctttgatatg acctgttttg gttttttgtt
    26641 tgtgttatcg acgatcaccg ctccagtcga atcaacgatt ttcgtgataa gatgcggcgt
    26701 atacatctga ccatcattgg caaatgcact gtaagcaccc gccatgatca atggagatac
    26761 tcctttttcc agtccaccaa gggctaagcc ataatactta tcagattttg ctaaaggcag
    26821 gccgaattct tctgctttat catatccttt ttgtaaaccg atctcatgca aaagccatac
    26881 agtaggcaaa ttcaaacttt gtgcaacagc ttgatacatc ggtacttctc cttggtatgt
    26941 cccgtcaaaa ttatgcgcat cgtaataact ttgcggttcg tctttcaaga tactggaagg
    27001 cttgtatcca gcttccagtg ctggtgtata tacgcttaat ggtttgatcg ttgatcctgg
    27061 tgaacgacgc atctgtgtag caaagctgaa tcctctaaag acatgatctc ctcttcgtcc
    27121 tactagtgct tgtacaccac ctgtattcgg atctaaagct actgaagcag attggaccat
    27181 ggcaccatct gaagcatttg ctgggaataa tgcactgttt tgataagtag cttctaattg
    27241 cttttgataa tcctgatcta aagctgtata gattttatag cctttattca tgacatcttc
    27301 ttcatcaaga ccatactcgt taactgcttc atcgatcact gcatcaaaat aataaggata
    27361 acgataacca gaatctgaat tatcgtatgt gtcattaaga tacgcgctga tatctgtctg
    27421 gctttctgct tgttcttgtt cttttgtgat ttttccgttt tcttccatta cacttagtac
    27481 tgtgtttttc cgatcattcg cattttttgg gtgatcgatc ggattataaa taccaggacc
    27541 tttcagcatt cctgccaaag tagcagcctc agacagcgta acattcgttg catctactcc
    27601 aaaatacttt ctggaagcgt cttggacacc ccataccccg ttgccaaaat aggcattgtt
    27661 aagatacatg gtcaagattt cttctttgct gtacttcttt tcgatctcga ttgccaaaaa
    27721 taattccttc gcttttcgat caaacgtttg gtctaatgtc agatacgcat ttttggcaag
    27781 ctgctgagtg atcgtacttc ctccgccccc tgatgttccg ccagacaaga ccattcggac
    27841 tgctgcacgg gcaatccctt tgatatcaaa cccgtgatgc tcgtaaaaat tcctatcttc
    27901 tgtcgagatt actgcatctt ggatataggg agaaatcgcg tttaattcta catatgttcc
    27961 tttttgggcg tacagtgtgc cagcttcttc gtttttctta tcataaatga ctgtcgattg
    28021 gcttaatcct gattttaacg tttcgacatt tgcttgtttt gctaaaataa agaggtagat
    28081 acttgtcact aagacacaga tcaaaccaat cagcaaaatc aatttgttca catgatattt
    28141 tttccagatg cgctttcgcc atcgatggaa ttgtccgaga tatggtctga tccaacgcca
    28201 aaaactgatc aaagcagctt ttatcttttc taaaatgctt tgaaaatcca tttgcttctt
    28261 ctcctgtcta tttgattcaa tcgtcttatt ttaccatagt gaaagagtgt tttcttcctg
    28321 cttaagttgt agttcaatat gtttttttac gttagtgcaa gtagagtaac ataagaatga
    28381 acagaaaaat gtaagttttt tcttaaatat gttttaaaaa tggtacaatt agtctgcgat
    28441 tgcactgaaa ggagtacact tgtgaaaatc acgatcactt tacctatgaa ccatcctgaa
    28501 acaacaattc gcgacttgct tgaaaaggaa tggttagttc ctagaaaggt tcgacacttt
    28561 ttgcgtatac gtaaaaacgt ttggatcaat caacaacctg ctttgttcca tcatgaagta
    28621 aagggcaatg accagatcac attgttattc gaagaaacag attataatta tcaagaagtt
    28681 caattaggag atgcgaagca catccgtgtt ttatatgaag atgagcattt gattatagtg
    28741 gataagcctg caggaatgaa gactcatcca aatgaaccga cagaaaacag taccttactc
    28801 aatcatttag ctgcttatct cgacactaaa cagcaacgac cttacgttgt ccaccgcttg
    28861 gacaaagaaa caagcggtgc cgttttattt gcaaaaaatc catttgtctt gccgatactt
    28921 ggaagaatgt tggaacaaaa aatgatctat cggagatatc aagccatcgt atggggagaa
    28981 attgccaaag atatgaccat caccaataaa attggacgag atcgccatga tcggagaaaa
    29041 agggtcgtag atgataaaaa tggtcagagc gccttgacac atgtcactgt caacaaagta
    29101 ctttctgaac aaacacaagt ctattgtgtt ttggaaaccg gacgtacaca ccagatcaga
    29161 gttcatttag ctcataaggg acatccgatc gttggagacc ctctctacca aaatagaaca
    29221 gctgaacgac tgatgctcca tgcattagaa ctgcacatga tccatccatt tactcaagaa
    29281 aaaatagtcg gtaaagcttt acctggatta tgggaataga aataaaaaag aacagagtct
    29341 tatcagcaga taatgataag actctgttct ttttgtgtaa tttgtctgct tacttttgat
    29401 gttcgtgttc tgctaacagc aaactaccga taatcccact ttcattcggc agtccccaac
    29461 gaacgatata ttcacttact tgaggtgttt ccatataacc acccatcaat tcaacaaatt
    29521 gctgacggat tttttgaagg agatgatcct gattcattac cccaccgcct aaaataatct
    29581 tttcaggtgc taacgtcaac gtatagttga tcagtgcttg agcaatataa taagcctgaa
    29641 tagcccaaac aggatgatct tgcggcagat cttgcccttt gatccctgtg cgtgcctcaa
    29701 tagacggtcc tgctgctaat ccttctaaac aatctttatg ataaggacaa gtaccttcat
    29761 aatggtcatc tggatggcgt ttcacccaaa tatgtcccat ttcaggatgt gcgatccctg
    29821 aaaaaatttc ttcattgatc acgacacctg caccgattcc tgttcccacg gtaaggtaaa
    29881 tacaactatt ttttccttgt gctgctcctt gcttcaattc cccatatgca gcagcattga
    29941 catccgttgt ccaagcagca ggaatattgt agcgttcctt gattgtgcca agaaaatcga
    30001 attgtgccca cccttttttg ggagttgcca aaacgtatcc gtaattatct tttgttttgt
    30061 cgataccaat cgggccgaat gagccaatcc cgattccctt caattcataa cgatcaaaaa
    30121 attcaaatac ctgatccaat gtttcttctg gtgttgtggt agggatactt aattt
//
LOCUS       DAZKMB010000020        28125 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000020 DAZKMB010000000
VERSION     DAZKMB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.020"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1877
                     /locus_tag="ACG8V3_000844"
     CDS             237..1877
                     /locus_tag="ACG8V3_000844"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0299407.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="ACG8V3_000845"
     CDS             2031..3353
                     /locus_tag="ACG8V3_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0299408.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="ACG8V3_000846"
     CDS             complement(3415..3846)
                     /locus_tag="ACG8V3_000846"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0299409.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="ACG8V3_000847"
     CDS             complement(3910..4746)
                     /locus_tag="ACG8V3_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0299410.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="ACG8V3_000848"
     CDS             complement(4936..5238)
                     /locus_tag="ACG8V3_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0299411.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG8V3_000849"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG8V3_000849"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0299412.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="ACG8V3_000850"
     CDS             complement(5692..5958)
                     /locus_tag="ACG8V3_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0299413.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="ACG8V3_000851"
     CDS             complement(6212..6937)
                     /locus_tag="ACG8V3_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0299414.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="ACG8V3_000852"
     tRNA            complement(7303..7373)
                     /locus_tag="ACG8V3_000852"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="ACG8V3_000853"
     CDS             7490..8242
                     /locus_tag="ACG8V3_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0299415.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="ACG8V3_000854"
     CDS             8258..8737
                     /locus_tag="ACG8V3_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0299416.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="ACG8V3_000855"
     CDS             complement(8988..10568)
                     /locus_tag="ACG8V3_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0299417.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="ACG8V3_000856"
     CDS             complement(10561..11304)
                     /locus_tag="ACG8V3_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299418.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="ACG8V3_000857"
     CDS             complement(11480..12256)
                     /locus_tag="ACG8V3_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0299419.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="ACG8V3_000858"
     CDS             complement(12504..13340)
                     /locus_tag="ACG8V3_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0299420.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="ACG8V3_000859"
     CDS             complement(13351..14280)
                     /locus_tag="ACG8V3_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0299421.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="ACG8V3_000860"
     CDS             14472..14825
                     /locus_tag="ACG8V3_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0299422.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="ACG8V3_000861"
     CDS             complement(14899..15555)
                     /locus_tag="ACG8V3_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0299423.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="ACG8V3_000862"
     CDS             15781..17715
                     /locus_tag="ACG8V3_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0299424.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="ACG8V3_000863"
     CDS             complement(17801..19024)
                     /locus_tag="ACG8V3_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0299425.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20889)
                     /locus_tag="ACG8V3_000864"
     CDS             complement(19018..20889)
                     /locus_tag="ACG8V3_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0299426.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="ACG8V3_000865"
     CDS             complement(20910..22028)
                     /locus_tag="ACG8V3_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0299427.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="ACG8V3_000866"
     CDS             complement(22111..22944)
                     /locus_tag="ACG8V3_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0299428.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="ACG8V3_000867"
     CDS             complement(22937..23854)
                     /locus_tag="ACG8V3_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0299429.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="ACG8V3_000868"
     CDS             complement(24081..25205)
                     /locus_tag="ACG8V3_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0299430.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="ACG8V3_000869"
     CDS             25388..26296
                     /locus_tag="ACG8V3_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0299431.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="ACG8V3_000870"
     CDS             26437..28050
                     /locus_tag="ACG8V3_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0299432.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8750 a         5546 c         4798 g         9031 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagg
//
LOCUS       DAZKMB010000021        28055 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000021 DAZKMB010000000
VERSION     DAZKMB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.021"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            854..2023
                     /locus_tag="ACG8V3_000871"
     CDS             854..2023
                     /locus_tag="ACG8V3_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0299433.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            2496..3050
                     /locus_tag="ACG8V3_000872"
     CDS             2496..3050
                     /locus_tag="ACG8V3_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0299434.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(3223..3660)
                     /locus_tag="ACG8V3_000873"
     CDS             complement(3223..3660)
                     /locus_tag="ACG8V3_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0299435.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3708..4655
                     /locus_tag="ACG8V3_000874"
                     /pseudo
     CDS             3708..4655
                     /locus_tag="ACG8V3_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4926..5361
                     /locus_tag="ACG8V3_000875"
                     /pseudo
     CDS             4926..5361
                     /locus_tag="ACG8V3_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            5377..6210
                     /locus_tag="ACG8V3_000876"
     CDS             5377..6210
                     /locus_tag="ACG8V3_000876"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0299436.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            6455..7480
                     /locus_tag="ACG8V3_000877"
     CDS             6455..7480
                     /locus_tag="ACG8V3_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0299437.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7618..7743
                     /locus_tag="ACG8V3_000878"
     CDS             7618..7743
                     /locus_tag="ACG8V3_000878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299438.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7768..8883
                     /locus_tag="ACG8V3_000879"
                     /pseudo
     CDS             7768..8883
                     /locus_tag="ACG8V3_000879"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8966..9862
                     /locus_tag="ACG8V3_000880"
                     /pseudo
     CDS             8966..9862
                     /locus_tag="ACG8V3_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9878..10702
                     /locus_tag="ACG8V3_000881"
     CDS             9878..10702
                     /locus_tag="ACG8V3_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0299439.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10734..12044
                     /locus_tag="ACG8V3_000882"
     CDS             10734..12044
                     /locus_tag="ACG8V3_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFQ0299440.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12192..13451
                     /locus_tag="ACG8V3_000883"
     CDS             12192..13451
                     /locus_tag="ACG8V3_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0299441.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            13461..14333
                     /locus_tag="ACG8V3_000884"
     CDS             13461..14333
                     /locus_tag="ACG8V3_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0299442.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            14345..15175
                     /locus_tag="ACG8V3_000885"
     CDS             14345..15175
                     /locus_tag="ACG8V3_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0299443.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            15215..17398
                     /locus_tag="ACG8V3_000886"
     CDS             15215..17398
                     /locus_tag="ACG8V3_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0299444.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            17459..18679
                     /locus_tag="ACG8V3_000887"
     CDS             17459..18679
                     /locus_tag="ACG8V3_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0299445.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18676..20136
                     /gene="gtfA"
                     /locus_tag="ACG8V3_000888"
     CDS             18676..20136
                     /gene="gtfA"
                     /locus_tag="ACG8V3_000888"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFQ0299446.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            20213..21319
                     /locus_tag="ACG8V3_000889"
                     /pseudo
     CDS             20213..21319
                     /locus_tag="ACG8V3_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21641..21850
                     /locus_tag="ACG8V3_000890"
     CDS             21641..21850
                     /locus_tag="ACG8V3_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0299447.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21843..21992
                     /locus_tag="ACG8V3_000891"
     CDS             21843..21992
                     /locus_tag="ACG8V3_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0299448.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22146..22811
                     /locus_tag="ACG8V3_000892"
     CDS             22146..22811
                     /locus_tag="ACG8V3_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0299449.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23095..24180)
                     /gene="xerS"
                     /locus_tag="ACG8V3_000893"
     CDS             complement(23095..24180)
                     /gene="xerS"
                     /locus_tag="ACG8V3_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0299450.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            24514..24747
                     /locus_tag="ACG8V3_000894"
     CDS             24514..24747
                     /locus_tag="ACG8V3_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFQ0299451.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24744..25151
                     /locus_tag="ACG8V3_000895"
     CDS             24744..25151
                     /locus_tag="ACG8V3_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0299452.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(26288..26806)
                     /locus_tag="ACG8V3_000896"
     CDS             complement(26288..26806)
                     /locus_tag="ACG8V3_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0299453.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27173..27421
                     /locus_tag="ACG8V3_000897"
     CDS             27173..27421
                     /locus_tag="ACG8V3_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299454.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            27924..>28055
                     /locus_tag="ACG8V3_000898"
     CDS             27924..>28055
                     /locus_tag="ACG8V3_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0299455.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     "
BASE COUNT         9618 a         4250 c         5140 g         9047 t
ORIGIN      
        1 aattttctac gttttccttt tcatatatat taaatattac ggaaaattat ataaactaat
       61 ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt taggtctatt
      121 taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc tttcgtactt
      181 aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac tagtatgata
      241 tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt cctaaaacct
      301 ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa catgaaatta
      361 tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa catttattga
      421 ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt gataataaag
      481 tactacctat taaaggtagt actttattta atttagtata aattaacata ctactatatt
      541 ttaggtttca tattatataa attaataata tactaccatt tgttggtagt ataatttaaa
      601 ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa gtttaataag
      661 agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct ttagtgatat
      721 aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa tagatattta
      781 gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg aaattttata
      841 ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa tcaataaagg
      901 tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat tattgcaggc
      961 tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca attcaggtaa
     1021 ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat taaatttggt
     1081 aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt ataaaagaac
     1141 caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta ctatgtctga
     1201 aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa ccatttcaaa
     1261 tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct tagaatccag
     1321 atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa ctgtttcaaa
     1381 agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg ttcttgggtt
     1441 tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag acctaaaaga
     1501 ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca ttaacgatag
     1561 tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata tatctagaaa
     1621 tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt tcaaattagt
     1681 ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga tagataaatg
     1741 gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat taacctttta
     1801 taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg aaggttttaa
     1861 caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag aatctctgga
     1921 gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc gagttcataa
     1981 aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc aacagaatgg
     2041 gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct tgcgtaaagt
     2101 tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg ttattcatat
     2161 gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa acttaaattt
     2221 catataattt aattatgtaa attagctatt ctaagaactt ctacggataa ctgcccgtct
     2281 aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt tgtacaaaaa
     2341 agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc ccttgatttt
     2401 attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat atacataatc
     2461 attaactcag aatattttta agaagggaaa caatagtgag ctataatgta caaccgttaa
     2521 ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt aatccggaaa
     2581 gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca gatattatag
     2641 gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa cataaaacag
     2701 gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta tatactgaag
     2761 gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat ataacaaggg
     2821 atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat gatataggaa
     2881 cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga gacattgcta
     2941 ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac attggtataa
     3001 ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa tttaaaaatt
     3061 tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat tttatttcat
     3121 aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt gttatataaa
     3181 ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa agagaatcaa
     3241 tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact caacgtttag
     3301 cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt tgaccttctg
     3361 caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa ttgccgtttc
     3421 ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt taatatctgt
     3481 atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga gcagactaca
     3541 atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt taatgatgat
     3601 tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg atgaaaacaa
     3661 aagttgttca ccatctttta taggtttgat tctcattttt agatattatg cccgcacgta
     3721 ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt tatcataata
     3781 tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc atgtatcacc
     3841 tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt gaagtacaag
     3901 aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta aaagttcctt
     3961 ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt tgccattatg
     4021 gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat gcaacacgat
     4081 tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg tatgtcacag
     4141 accagatccc tacagagcat gccattttag gaattgatgc cattcaacta cgcgtacaat
     4201 attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa gaatacgtca
     4261 gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact cagttctatc
     4321 aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg attggtagcg
     4381 tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc gacaaacaat
     4441 tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt gtatcacttt
     4501 attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga aacctagatg
     4561 caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt ttaccgcagt
     4621 acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc catgacgaag
     4681 agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc cattctaaag
     4741 aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta ataaaactac
     4801 ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt aaactaaaca
     4861 tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat aaaggggagt
     4921 accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa aaatgtcagt
     4981 ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc aggaaaatca
     5041 tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga cacctatgat
     5101 agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga gattgataat
     5161 ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg ctcaacgtta
     5221 gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt tgcaacacct
     5281 agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg ttctgtctgt
     5341 cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc ggctattgat
     5401 aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg aattgaggaa
     5461 caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc agaagatgtt
     5521 aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt acgtgtaaaa
     5581 gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga tacgcatctt
     5641 tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt aaatacaaat
     5701 gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca tgagaaaata
     5761 gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga acgacatcaa
     5821 gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg gatatataac
     5881 aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat tgttgttact
     5941 ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa acgagtgatg
     6001 gaacaattat cgattattgg atttgatgat attacttttg cagaattaat ggatcctgcg
     6061 ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca ccaactgatt
     6121 ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg agaattgatt
     6181 tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt atagttaaca
     6241 ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc gtcgcaaatt
     6301 cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt ttttaattat
     6361 gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga ttatatgtta
     6421 gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg atgttgcaaa
     6481 acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc cgacactttc
     6541 agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc attatgtcaa
     6601 aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag tttctgaagt
     6661 agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag tcgaagcaga
     6721 acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt tggagcaaga
     6781 cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt ctgtgattga
     6841 agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta ttgaagctga
     6901 agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag atcgattgta
     6961 taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt tagatagcca
     7021 aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga cgtggatgaa
     7081 acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca cacttgacgg
     7141 aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc cgactgctat
     7201 catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa aaaatggtct
     7261 acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg ctgaattttt
     7321 aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac tagcagttcg
     7381 catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga ttgtttctaa
     7441 taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac atccaaaaaa
     7501 gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa aacattaagt
     7561 aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg aattaagatg
     7621 aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac attactcgca
     7681 gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag ctatgcgagt
     7741 tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga aaaacaaaat
     7801 cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt taaattagaa
     7861 gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag cgaatcctat
     7921 aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga aaatgatatt
     7981 gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga cgaactatac
     8041 gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt atttgataat
     8101 gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca agctgcaatt
     8161 gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg aacagagact
     8221 ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga caatacaaaa
     8281 tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga tattgcactt
     8341 gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt aagtcgtttt
     8401 caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg atttttgaaa
     8461 aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa agttaatgca
     8521 acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc agaagaagtg
     8581 aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc ggaaacgggg
     8641 gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc agatacaatt
     8701 gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata caataaagat
     8761 ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc tggtgaagta
     8821 actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt gagcggaaat
     8881 taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg tatattaatg
     8941 gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat atcgtaaaga
     9001 tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa ttttattaaa
     9061 tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag ggttcggtga
     9121 tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg aagtttttca
     9181 gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg ttgtattatc
     9241 cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt atcggacaat
     9301 ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc gctggatgtt
     9361 caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg atggaccagc
     9421 atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag tttggagtgc
     9481 tattggttat aacatggtgt tattactagc cggcttataa gaaattccta aagattatta
     9541 tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca ttacgttacc
     9601 attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg ctttccaagt
     9661 atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta aatcacaatc
     9721 attagcctat ttattctata aacattcatt tattttaaat gacaagggat atggttcggc
     9781 aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa tgaagttgca
     9841 gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat ttttttcaaa
     9901 tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac cttttatttg
     9961 gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac ctacaatttt
    10021 ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac cattcatggc
    10081 tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg ttttattttc
    10141 agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata tccttttcag
    10201 cttagtactt attcaaatga tgattcccgg ttccattttt attactccac agtatttatt
    10261 agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag gggtagttag
    10321 tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg aattggagga
    10381 agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta tgttgccgct
    10441 tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat ttaaagattt
    10501 aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc cgggattagc
    10561 aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt cgttattagc
    10621 aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg aaggaattgc
    10681 tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag tacatgacaa
    10741 aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta ggcgatgcga
    10801 tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat gaaaaacgac
    10861 taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa aatcgtgctg
    10921 atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt gttatcaatg
    10981 ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt cctaaaaaat
    11041 atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt agaaccctac
    11101 ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt ccaaatgctt
    11161 ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta aaagcgacga
    11221 gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa ttgtttgagc
    11281 atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt gcaggcatta
    11341 atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat ccaacaattc
    11401 gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt gaattgttga
    11461 aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac catccatact
    11521 ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg gatgaatact
    11581 tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac attgtaaata
    11641 atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg gattctatta
    11701 cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta atcacaaact
    11761 taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc gtgcaaccaa
    11821 cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat atcaatgttc
    11881 aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat caagcagcgt
    11941 atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat ttagtggatg
    12001 atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa gctgaaagag
    12061 taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat attgacaaaa
    12121 cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa ttgtataagg
    12181 aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca ctaggagcaa
    12241 cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag gtagaaatag
    12301 agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct aaagattttg
    12361 aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct ggtgaagtaa
    12421 ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat cctcaaagta
    12481 tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa gaaccgtatt
    12541 tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt gtatatagca
    12601 taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt gaggaaatgg
    12661 ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag attaaagatc
    12721 aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat ggttatcacc
    12781 aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt tggcgtttct
    12841 cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt aatcgattag
    12901 atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt tataacgata
    12961 cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca tgggcaatgc
    13021 cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt ccagcagatg
    13081 aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt tctgcaactt
    13141 ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct gaagcgatgc
    13201 agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc gagaatactc
    13261 aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat ttagtttggt
    13321 tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat tatttacaca
    13381 atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca atgaaagctg
    13441 acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac tgggcaatca
    13501 tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca atggttcaag
    13561 gtgcttttta cagtttaact aattggactg gtctgactta taattttgat tttgtgggtt
    13621 tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt ggcttcactt
    13681 taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg attgcgcggg
    13741 cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt tttccagcgg
    13801 tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat ggtttaccaa
    13861 aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga acaacatggg
    13921 gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg ccagtcatca
    13981 ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct agaatggatg
    14041 gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta ccaagtattt
    14101 caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa atttttgctt
    14161 taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt tataattatg
    14221 cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta tttatcttaa
    14281 ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc taggaggttt
    14341 cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat tattaggggg
    14401 cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa caacacccga
    14461 tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt tccgaagatt
    14521 gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa tcattgcagt
    14581 agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa aaatgactcg
    14641 tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg ttcctttcca
    14701 agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa atatgtgggg
    14761 attaatctta ctctatctca cttatgccgt accacagaca ctatttttat atgttggttt
    14821 tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg gggctagtca
    14881 ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg cgacgactat
    14941 tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa ttttaaatcg
    15001 tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac aatattttaa
    15061 cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta cactagttta
    15121 tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga aataaccaat
    15181 aaattattct cattataaag gagttcataa acttatgatt atatttgaca atcacacaca
    15241 aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag aaaataatta
    15301 tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt atcgctaccc
    15361 ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt tattatcttt
    15421 agacacatta ccattagaat ttccaggata tggacatggt gattttagag aaccggcgca
    15481 taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata gctacgaaat
    15541 tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag atgatgatga
    15601 agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt tacagttgtt
    15661 ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag taaataatag
    15721 tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc caaatcgtcc
    15781 actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga ctagagaaaa
    15841 aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc atcaacaaaa
    15901 tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg catatggatt
    15961 taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat ttgaccaaac
    16021 acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa ctggcgactc
    16081 atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata cgttatcaca
    16141 gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt taaaagaacg
    16201 tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag aaaaaatcat
    16261 gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag atgatggttg
    16321 gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa cagaaggcaa
    16381 acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga tgaaatttgg
    16441 gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag cacatccaga
    16501 ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat atgttcttga
    16561 tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga tttttgatcg
    16621 tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag tctattctgt
    16681 cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag ggttatatga
    16741 atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt gttcgggtgg
    16801 aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga cgagcgacaa
    16861 taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt atccaatttc
    16921 aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag aaacgagttt
    16981 aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag atctaacgca
    17041 attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata aaaagcatcg
    17101 ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag gcaatgatac
    17161 tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt tccgagtatt
    17221 agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag aagaagcaac
    17281 ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg gtttttatat
    17341 tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac agatatagta
    17401 gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg aagaaaatat
    17461 gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa atgaagaagt
    17521 acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag gcggatttag
    17581 aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg aatctgatgg
    17641 agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca ttgaagtgtg
    17701 gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg tagcagaagc
    17761 cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt attcgatggt
    17821 ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt ttaaagatag
    17881 ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag ttacgcaaga
    17941 taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa ttaataaata
    18001 tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc tttttatgtt
    18061 actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca acatagaaat
    18121 ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac gggcattaga
    18181 atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc gggataattc
    18241 tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt ctgataatgt
    18301 atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt taaccaatca
    18361 acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata gtgtatacaa
    18421 agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac atattttgct
    18481 ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta cagcaacaga
    18541 acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt atgaagatgt
    18601 tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg tattagctaa
    18661 ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta tgcagatagt
    18721 ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca aggtgttatt
    18781 ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt tgcgccgagt
    18841 gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc attaggtgaa
    18901 gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc aaaatttttc
    18961 caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat ccgtattcat
    19021 gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta caagcgtaaa
    19081 gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga ggtttggaat
    19141 acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa agaatttatt
    19201 cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt agatgcgttt
    19261 gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga tatttgggaa
    19321 cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt accagaaatc
    19381 catgagcatt attccattca aatgaaaatt gccaatcatg attattatat ctatgacttc
    19441 gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg tttagccaat
    19501 tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga tggaattggt
    19561 gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc tgaggaattg
    19621 tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa taatttagat
    19681 atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg tagctatctg
    19741 ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta cgttgggtta
    19801 ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg taatattaat
    19861 cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt gattcaacaa
    19921 ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga tggaacgatt
    19981 gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa aaacggtagt
    20041 gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga gtctaaagat
    20101 ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa caaaaaatcc
    20161 tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat acatggataa
    20221 gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag gaaaactttc
    20281 tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac tgtcttttga
    20341 agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt tgcgtgattt
    20401 gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat cgattagtca
    20461 tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag aaatttttca
    20521 acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc atctgtatct
    20581 gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga ctttccgtca
    20641 aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata ctacgatgta
    20701 tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac taccagtatt
    20761 ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact ttgaaaagat
    20821 ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa acaccaaagt
    20881 tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag acgagctggt
    20941 tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg ttcaagataa
    21001 gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa aagaaatctc
    21061 tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca aacaacacat
    21121 gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat tgattttaac
    21181 gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg aaaaatcagt
    21241 gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat gttgtctaga
    21301 aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt tttcagttga
    21361 ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag ttttttttga
    21421 cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca acactagtga
    21481 tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc aagtatacaa
    21541 agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact atttttagta
    21601 gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta taacaaaaaa
    21661 tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac gacaggcaaa
    21721 agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag gactagtacc
    21781 tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg attttcagaa
    21841 tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct agtttgggat
    21901 tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa cagttaaaga
    21961 agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga aacttctcaa
    22021 agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta tcaagggaaa
    22081 gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt tcttatttct
    22141 ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa aataatttat
    22201 tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag atgtcattag
    22261 atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt ggaaatgtga
    22321 agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa gttatttcaa
    22381 ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg ttacttttta
    22441 tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt gattttgaaa
    22501 ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc tatgagtttg
    22561 tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat acaattacct
    22621 ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt gtactttctt
    22681 ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga catgcaacga
    22741 tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct tctttgctta
    22801 tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct gatgtacaaa
    22861 cgactttagg aacttatggt catttgtatc taaattcatt gagagagata acaaatgatc
    22921 ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt tacgataata
    22981 aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag aaaaactata
    23041 atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt atactcataa
    23101 agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag ttgttgtatt
    23161 ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct gttggtagag
    23221 tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa agccttttga
    23281 atattttgct accatccgtt cgattgcaat accggatata cgcttggctt ctccttggta
    23341 aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt tccgaatagc
    23401 tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat ctcttttatt
    23461 tccttttcta gttactacca cattattctt atctaaagat aggtcatcta aattaatatt
    23521 tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg ccaggtctct
    23581 ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt tatattcatt
    23641 ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt tctcaatggc
    23701 attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat ccataggatt
    23761 attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat atttaaatag
    23821 aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta agttattatt
    23881 atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc gacgtttcaa
    23941 atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg tgatagaagt
    24001 atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat tttttagata
    24061 ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa aatcttttat
    24121 ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg tatttttcat
    24181 gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga ctataccaat
    24241 tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc aaaaaaagca
    24301 tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata gggaaggaaa
    24361 tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga aagaacaatc
    24421 tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca agcgtatata
    24481 ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag aaaatttagg
    24541 gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga aaatgggtta
    24601 aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat tacaaaaaaa
    24661 caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga atatgatact
    24721 acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa gatattataa
    24781 aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt gtaaaggatg
    24841 cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt gaagaggttt
    24901 atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag aaacatcctt
    24961 ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg aaaatcaatg
    25021 gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat attgctacgt
    25081 atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag acacatacgc
    25141 agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct atagcgaaat
    25201 aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa acgtaaattt
    25261 tctatatagt tggattaaga taacgactat ttattttatc atatttttac tataccctat
    25321 acttttactt ggtatattta tgaataatcg caaatttacc cctatactac tagtttgtaa
    25381 gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc tacaacttat
    25441 aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag taaagtttgt
    25501 tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc tgaaaactta
    25561 tgaaatgaaa gaccttttac atcttgcaga aatcgttact gggattaaac taagcaataa
    25621 aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat tctgataaat
    25681 gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga tctcttcagt
    25741 atcagtaata ggataggcga taggccacat accctttgat ggttagaata aactgctcaa
    25801 aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa ttataaacca
    25861 taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca gaagatataa
    25921 ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta aagggaattg
    25981 tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc ccctgctagt
    26041 ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga agtgagtcat
    26101 ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc tacggcattc
    26161 taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga acttatgtag
    26221 tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct tagcagtaaa
    26281 cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa gtcccaaatg
    26341 gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt ttggttgcct
    26401 cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt gaatcaatca
    26461 tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc aatggtagat
    26521 gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat ttaaattgat
    26581 cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt agaattaaat
    26641 tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact tcctcaactc
    26701 ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta gtataagtcc
    26761 tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata ttacctccat
    26821 aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt ttaatccgtt
    26881 ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga tttataaaat
    26941 atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa aatacgaata
    27001 tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta aatatcaaaa
    27061 tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta agaactgtta
    27121 agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga aagtggtaaa
    27181 actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg cgttgggtaa
    27241 aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc agatgaaaca
    27301 gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc gattgccata
    27361 ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc atccttttta
    27421 ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga tgttttcacc
    27481 agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga aaattcaccg
    27541 cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt ccaaaattaa
    27601 gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt ctttttaaac
    27661 aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt ttatgacttt
    27721 cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg gacgtttacg
    27781 agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg aactaccccc
    27841 acttagcttg gataggctac aattaactag acagaaaaat taaggtgtgt agactagaag
    27901 aaaacatacc aggaggaatt tttatgtcta agagaacacg aagaactttt tcacaagaat
    27961 tcaagcaaca aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa atcattcgag
    28021 aatatgaact aacggcttca gcatttgaca aatgg
//
LOCUS       DAZKMB010000022        27600 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000022 DAZKMB010000000
VERSION     DAZKMB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.022"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG8V3_000899"
     CDS             <1..1152
                     /locus_tag="ACG8V3_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFQ0299456.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8V3_000900"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8V3_000900"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0299457.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG8V3_000901"
     CDS             2728..3003
                     /locus_tag="ACG8V3_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0299458.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG8V3_000902"
     CDS             3009..3776
                     /locus_tag="ACG8V3_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0299459.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG8V3_000903"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG8V3_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0299460.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8V3_000904"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8V3_000904"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0299461.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG8V3_000905"
     CDS             7920..8222
                     /locus_tag="ACG8V3_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0299462.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG8V3_000906"
     CDS             8225..9388
                     /locus_tag="ACG8V3_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0299463.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG8V3_000907"
     CDS             9414..12842
                     /locus_tag="ACG8V3_000907"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0299464.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG8V3_000908"
     CDS             12863..13987
                     /locus_tag="ACG8V3_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0299465.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG8V3_000909"
     CDS             13968..14414
                     /locus_tag="ACG8V3_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0299466.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG8V3_000910"
     CDS             14398..14736
                     /locus_tag="ACG8V3_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0299467.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8V3_000911"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8V3_000911"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0299468.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8V3_000912"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8V3_000912"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0299469.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG8V3_000913"
     CDS             15893..16936
                     /locus_tag="ACG8V3_000913"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0299470.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACG8V3_000914"
     CDS             17095..17823
                     /locus_tag="ACG8V3_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0299471.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACG8V3_000915"
     CDS             17939..18184
                     /locus_tag="ACG8V3_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0299472.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACG8V3_000916"
     CDS             18181..18399
                     /locus_tag="ACG8V3_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0299473.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACG8V3_000917"
     CDS             18535..19074
                     /locus_tag="ACG8V3_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0299474.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACG8V3_000918"
     CDS             19067..21340
                     /locus_tag="ACG8V3_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0299475.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACG8V3_000919"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACG8V3_000919"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0299476.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8V3_000920"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8V3_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0299477.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACG8V3_000921"
     CDS             complement(23167..23436)
                     /locus_tag="ACG8V3_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299478.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACG8V3_000922"
     CDS             complement(23698..23826)
                     /locus_tag="ACG8V3_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299479.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACG8V3_000923"
     CDS             complement(23906..24361)
                     /locus_tag="ACG8V3_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0299480.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..25725)
                     /locus_tag="ACG8V3_000924"
                     /pseudo
     CDS             complement(24499..25725)
                     /locus_tag="ACG8V3_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(25731..26231)
                     /locus_tag="ACG8V3_000925"
     CDS             complement(25731..26231)
                     /locus_tag="ACG8V3_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0299481.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            26759..26842
                     /locus_tag="ACG8V3_000926"
     tRNA            26759..26842
                     /locus_tag="ACG8V3_000926"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26793..26795,aa:Leu,seq:cag)
     gene            complement(27166..>27600)
                     /locus_tag="ACG8V3_000927"
     CDS             complement(27166..>27600)
                     /locus_tag="ACG8V3_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0299482.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         9180 a         4770 c         5822 g         7828 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa ataggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acatactttt ttacatcagt
    25741 ttgattttcg tatagtattc tttctcatct tcacgataaa tcgcagcccc atttttttgt
    25801 tgtgctttaa gactgccaag atttttttta tttacagaca aaagtgcctc atcaatgccg
    25861 attttttttg cctcttgtag tactaatgct aatccttgtg tggcatatcc ttttttacga
    25921 taattagggg agatgccgta accaatatgg ccagcgccgt ttcttaatgt ttcgttcagt
    25981 ttatggcgca aattaaaaat tcctacatac tcatgttcat cactttctaa aataaagacg
    26041 gttgcgggaa cgtaactctc aggaagatcg attcctaaag acgttttttc gcattttttt
    26101 acataatcta aaaattcagc aaatgtatag ccataagcat tgttgataaa tccactctca
    26161 tttggtccaa agctagtttg tagctgataa gctttcttta aatctgcttc ccatagtttg
    26221 atcagtttca tcgtattcac ctctttaagt aaccttattc atttgttttc tactatagca
    26281 ctattgttgt aaatagccat gcctattttt atttattgtg tcatatcgta ttaagaatca
    26341 tcgaacaata caaatatgaa ttacaataaa aaaattattt gaagttcgtt cattcgttac
    26401 ttatgatctc tagaagaaag aaaacgtgaa ttacttcagc gtctagaatt ccagaaggat
    26461 agtaatcaac agaagaaata atcaatatcc aaatgaaaga agatagctga atcgagaaaa
    26521 agaggaaaaa agaaatgaag aaaaaaataa aatagttact aatcccagag tcgttcgttt
    26581 aacattgaaa tcgatcatat ggataaaaca gattttttca ccaaccctca atagatgatg
    26641 gagttgctcg gatgaattat ttgtgtagat ggcataaggg agggcgtaca gaaatgaaaa
    26701 agaacaaaaa tatgttcttg acaaaaaaca catgtaatcc taaactaaaa gatgtgatgc
    26761 ggctatggcg gaattggcag acgcgctagc ttcaggcgct agtgagggtg acctcgtgga
    26821 agttcgagtc ttcttagccg cataatttgt ttttttataa agagaaggta cacctggagc
    26881 gatgagaaag aaaataacat attatctaaa agaatatagg aaaaatttta aacaacggaa
    26941 ccagattcaa ataaatcaaa aaaaatttgt gtgaaggtgg gacagtttaa aacctaatag
    27001 attgattcaa atagtagatt tatagattct gatacaagaa atactggcaa acgatagtta
    27061 aggaataaag actaaactta aactcaaaaa gaggtcatgc gaaagaataa atcgcatgac
    27121 ctcttactat attttgaatg tgtttaaacg ttcaaataat gaatcttagt accaaccgtt
    27181 tgcttcccag aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc
    27241 tgctactctt tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg
    27301 gtaacggccg atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc
    27361 gatccattct tttgcagagc ttgtacttgc tggagtagca gcagtttctg ttacagcagg
    27421 agcaggttgt gtttcagcaa caggcgctgc ttgtgtttcc gtttgaaccg tttcttgtac
    27481 aacaggttgt tcagcttgaa caggttcagc ttggactggt gcttcttgaa cggctgcttg
    27541 aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DAZKMB010000023        27540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000023 DAZKMB010000000
VERSION     DAZKMB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.023"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(176..1462)
                     /locus_tag="ACG8V3_000928"
     CDS             complement(176..1462)
                     /locus_tag="ACG8V3_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0299483.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="ACG8V3_000929"
     CDS             complement(1455..2723)
                     /locus_tag="ACG8V3_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0299484.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="ACG8V3_000930"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="ACG8V3_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0299485.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="ACG8V3_000931"
     CDS             complement(3495..3941)
                     /locus_tag="ACG8V3_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0299486.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="ACG8V3_000932"
     CDS             complement(4034..4279)
                     /locus_tag="ACG8V3_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0299487.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4394..4972)
                     /locus_tag="ACG8V3_000933"
     CDS             complement(4394..4972)
                     /locus_tag="ACG8V3_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0299488.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5049..5579)
                     /locus_tag="ACG8V3_000934"
     CDS             complement(5049..5579)
                     /locus_tag="ACG8V3_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0299489.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="ACG8V3_000935"
     CDS             complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="ACG8V3_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0299490.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6436..6831)
                     /locus_tag="ACG8V3_000936"
     CDS             complement(6436..6831)
                     /locus_tag="ACG8V3_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0299491.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6828..8240)
                     /gene="cysS"
                     /locus_tag="ACG8V3_000937"
     CDS             complement(6828..8240)
                     /gene="cysS"
                     /locus_tag="ACG8V3_000937"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0299492.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8237..8773)
                     /gene="epsC"
                     /locus_tag="ACG8V3_000938"
     CDS             complement(8237..8773)
                     /gene="epsC"
                     /locus_tag="ACG8V3_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0299493.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8859..9098)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9095..10555)
                     /gene="gltX"
                     /locus_tag="ACG8V3_000939"
     CDS             complement(9095..10555)
                     /gene="gltX"
                     /locus_tag="ACG8V3_000939"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0299494.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10725..11882)
                     /locus_tag="ACG8V3_000940"
     CDS             complement(10725..11882)
                     /locus_tag="ACG8V3_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0299495.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11916..13286)
                     /gene="radA"
                     /locus_tag="ACG8V3_000941"
     CDS             complement(11916..13286)
                     /gene="radA"
                     /locus_tag="ACG8V3_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0299496.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13349..13876)
                     /locus_tag="ACG8V3_000942"
     CDS             complement(13349..13876)
                     /locus_tag="ACG8V3_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0299497.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14106..14912
                     /gene="proB"
                     /locus_tag="ACG8V3_000943"
     CDS             14106..14912
                     /gene="proB"
                     /locus_tag="ACG8V3_000943"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0299498.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14909..16156
                     /locus_tag="ACG8V3_000944"
     CDS             14909..16156
                     /locus_tag="ACG8V3_000944"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0299499.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16232..16576
                     /locus_tag="ACG8V3_000945"
     CDS             16232..16576
                     /locus_tag="ACG8V3_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299500.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16693..17019)
                     /locus_tag="ACG8V3_000946"
     CDS             complement(16693..17019)
                     /locus_tag="ACG8V3_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299501.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(17634..18836)
                     /locus_tag="ACG8V3_000947"
     CDS             complement(17634..18836)
                     /locus_tag="ACG8V3_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0299502.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            18953..19468
                     /locus_tag="ACG8V3_000948"
     CDS             18953..19468
                     /locus_tag="ACG8V3_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0299503.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(19667..20113)
                     /locus_tag="ACG8V3_000949"
     CDS             complement(19667..20113)
                     /locus_tag="ACG8V3_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0299504.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(20160..21638)
                     /gene="msr(C)"
                     /locus_tag="ACG8V3_000950"
     CDS             complement(20160..21638)
                     /gene="msr(C)"
                     /locus_tag="ACG8V3_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0299505.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(21992..22621)
                     /locus_tag="ACG8V3_000951"
     CDS             complement(21992..22621)
                     /locus_tag="ACG8V3_000951"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0299506.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(22605..23069)
                     /locus_tag="ACG8V3_000952"
     CDS             complement(22605..23069)
                     /locus_tag="ACG8V3_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0299507.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(23556..24824)
                     /locus_tag="ACG8V3_000953"
     CDS             complement(23556..24824)
                     /locus_tag="ACG8V3_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0299508.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(25055..25792)
                     /locus_tag="ACG8V3_000954"
     CDS             complement(25055..25792)
                     /locus_tag="ACG8V3_000954"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0299509.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(25859..26428)
                     /locus_tag="ACG8V3_000955"
     CDS             complement(25859..26428)
                     /locus_tag="ACG8V3_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0299510.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            26556..27428
                     /locus_tag="ACG8V3_000956"
     CDS             26556..27428
                     /locus_tag="ACG8V3_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0299511.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         7758 a         5836 c         4959 g         8987 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tctccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
     4441 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
     4501 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
     4561 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aattgctaaa ggttcttgca
     4621 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
     4681 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
     4741 tgcttataac gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
     4801 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
     4861 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
     4921 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     4981 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     5041 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     5101 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     5161 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     5221 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     5281 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     5341 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     5401 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     5461 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     5521 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     5581 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     5641 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     5701 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     5761 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     5821 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     5881 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     5941 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     6001 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     6061 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     6121 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     6181 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     6241 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     6301 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     6361 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     6421 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     6481 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     6541 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     6601 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     6661 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     6721 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     6781 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     6841 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     6901 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     6961 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     7021 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     7081 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     7141 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     7201 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     7261 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     7321 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     7381 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     7441 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     7501 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     7561 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     7621 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     7681 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     7741 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     7801 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     7861 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     7921 ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     7981 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     8041 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     8101 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     8161 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     8221 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     8281 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     8341 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     8401 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     8461 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     8521 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     8581 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     8641 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     8701 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     8761 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     8821 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     8881 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     8941 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     9001 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     9061 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     9121 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     9181 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     9241 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     9301 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     9361 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     9421 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     9481 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     9541 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     9601 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     9661 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     9721 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     9781 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     9841 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     9901 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     9961 caaaattata agttggcata ccatcgcgtt tttggatgac aaagtcgcca ccgatattat
    10021 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    10081 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    10141 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    10201 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    10261 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    10321 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    10381 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    10441 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    10501 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    10561 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    10621 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    10681 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    10741 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    10801 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    10861 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    10921 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    10981 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    11041 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    11101 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    11161 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    11221 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    11281 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    11341 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    11401 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    11461 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    11521 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    11581 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    11641 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    11701 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    11761 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    11821 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    11881 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    11941 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    12001 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    12061 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    12121 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    12181 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    12241 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    12301 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    12361 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    12421 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    12481 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    12541 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    12601 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    12661 atcccatttg ttttagcggt tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    12721 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    12781 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    12841 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    12901 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    12961 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    13021 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    13081 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    13141 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    13201 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    13261 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    13321 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    13381 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    13441 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    13501 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    13561 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    13621 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    13681 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    13741 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    13801 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    13861 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    13921 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    13981 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    14041 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    14101 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    14161 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    14221 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    14281 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    14341 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    14401 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    14461 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    14521 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    14581 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    14641 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    14701 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    14761 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    14821 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    14881 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    14941 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    15001 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    15061 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    15121 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    15181 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    15241 cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    15301 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    15361 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    15421 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    15481 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    15541 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    15601 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    15661 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    15721 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    15781 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    15841 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    15901 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    15961 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    16021 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    16081 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    16141 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    16201 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    16261 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    16321 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    16381 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    16441 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    16501 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    16561 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    16621 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    16681 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    16741 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    16801 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    16861 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    16921 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    16981 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    17041 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    17101 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    17161 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    17221 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaat
    17281 cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat gagaagctat ttttccgttt
    17341 tgtttctcta atacttagga taaaacgctg attttacaac ctctgttaaa cggtgttcaa
    17401 tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt
    17461 tgtttctcta atactcagga caaaacgctg attttacaac ctcctgataa acggtgttca
    17521 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagaag ctatttttgt
    17581 ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctgat ttcttattct
    17641 tcttcatttg tagcaaaata attacggtga taaatgatac aacatacact gataagaaca
    17701 atggcacctg caaaataaaa cgcatagctt tcaccaatat gttcaccaat cacacctgca
    17761 ataaatggtg ctaaagctcc accgaaaaag cggacaaaac tatagctgga cgaagcaacg
    17821 tttcgttgca gacctggtat ttccatagca atcgtcgtca acagtgtatt gataattcct
    17881 tggaaaaatc ctgctaacac aatgctgatc ggtacaagga ctggcataga agttccagct
    17941 cctaataaca gcaaacaaat aatgaataag acaaatgctg ctaaaattgt ccgataagtc
    18001 gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag caatagccag aagcacaccc
    18061 cagccaaaaa atacaaaacc gacttctaat tcagaatagc caatcaataa aaatggcgca
    18121 tacgcaagta aagtaaagaa tccgaaatta tataacaatg cgattagtcc aactgaacga
    18181 agtttatgat tgcttaacgc tttgacacct tcccaaaggc ctacttttct ctttggtttc
    18241 tcagtttcag gtaaaagtac cgctagggct ataaaaccgc aaaacatcaa aaatgctacc
    18301 ccaaaaaatg gataacgcca tgaaccactg cccaagaccc caccgactag agggccaaca
    18361 gccattccgc agccaagtgc tgcttcatac atcatgattg ctttttctgt gttaccaact
    18421 aaaatactga cgatcgttgc taaagcagta gagacaaata aagcattccc gacaccccag
    18481 ccggcacgta acccaatcag cattccgata ttcccagata atccgccaag accagcgaat
    18541 acaacgatga taaacaatcc tgtcatcaat gtttttttca aaccgatccg actagataag
    18601 aaacctgtaa atagcatcac cacaccagtg actaacatgt aactagtaaa taacagggtc
    18661 gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt ttaagattgg gtctactaga
    18721 cccactccca taaaagcgat catacaggta gctgctacaa ctagcgctga acttttcccc
    18781 tttgtttctt catttgtttg ttcttgtata tctatttgat tctccaatat ttccatccct
    18841 ttctctctat tagcatcata catgatttat tttacatgtc aatatgcaca tgtaaaacgt
    18901 ggaaacttta ctgtgatata ataaagaaaa taagacgaga ggtaggattt ttatgaatac
    18961 tttagcctat atattattat gcgtgctttt atccaaacca cgttctgggt atgaattgaa
    19021 gcagctgatc acaatttttt gggaagccca tcacagtcag atctatacaa cgcttgccaa
    19081 gttagaccag cagggttatg tgaccgtttt ggacaaagac gagcactctc aaaaaaagat
    19141 ctaccacttg actgatgaag gacgtgctct tgtagaggaa tggatcaaag aggaaactcc
    19201 cgctcctacg caaaaagatg aatttctagc aaaaatctat gctttttcga cgctcgataa
    19261 aacaacagct actggactgc tattcactag agaacaacag ctgaacaaaa tcatgcaaaa
    19321 aaatcaaaaa aaattgaatg acctctcgct agacaagaaa caagaatttg gacgttatgt
    19381 tgtgatccag cgcagaatta tgctttgtca gcaggaactc caatggtgtc agcaagtaag
    19441 agaacaaatg cagcagtttt ttgagtaagg gataaacaga acctttctag aaatatctat
    19501 tgaagagcaa attatcttca ataaaagagc aatacagttg gaaaagaaaa agcgatccga
    19561 acgacaggcc aaaacctatc gttcggatca ctatttttct agaactgcat tcaagagact
    19621 atgacaaaaa gcatgccaag gccttttttc aaataacagt gttccatcat tcattacttt
    19681 gaattgcttt tagcacactt cttgaaagcg ggccaacgat gatcaactgc aacggtaaag
    19741 cggcaatcac attcattttc caagcattca tataagcaga gaataattga tcaggtattc
    19801 cttgttccat gatcacaccg aataaagaca tacatgtcac cattcctaaa accatcaagc
    19861 atgaaatagt taagatcatt tggaccttgc tttctggatt tactggtagt ttaaaggcaa
    19921 tttttttcgc cagcacaccg atgatgaaga catccaaaat gaaagccacc acgaatccag
    19981 gcactagacc aatcaacaaa cttgataaat gaagatcttg atgcagccat aaattatacg
    20041 cgctcatccc caagaccatc aaaaaacaca tcattgttgt aaaaattatt ccttcttttt
    20101 tatttgtagg caatctgtac cactccttta aatttgtcac gaaaatcatc gtagcacatt
    20161 taaaaatttc tcgtaagtac tttttttatt ttctgccctt ttatttcata aacttcatca
    20221 gccgtacgct cgacaaagta agaatcatga gaagtaaaca aaatcgttcc cggatatatt
    20281 tgcattagct tctctaaagc ttcgatcgtt gccatatcaa taaaattagt cggttcatct
    20341 aacagcaaca cattacttgg cttagtaaaa agcaaagcaa tcgtcagacg ggtcgcttct
    20401 cccccactta atgtacataa cggtttcgtc aaagtttcct gagcaaaccc taagtgattc
    20461 agcaaactac ggacaagcga ttctgaaaac tctgtttgtc tcatgacaaa acgaatgatg
    20521 gattcttcag atgtcatttg ataacccttt tgctgatata cttgaaagct tactttcgga
    20581 gagagaagga ttccttctcc ttgtttgcgg atatgttcta ataaggttgt cttacctgaa
    20641 ccattttcac cgacaatcgc gattttttta cctaaagaaa actgaaaatc accatttacc
    20701 agtaatgttc tcccacttct ttccaattgg acattttgtc ccatgatggg atagcggctg
    20761 tggagttcaa gagattttgg tactggaaaa cggatttgtt tcatctgctg cggcttggtt
    20821 gtttctccta ttttttggag tcttctctct aatgttttcg cattcttttg gatggctttt
    20881 tgtacactgt ctttttgttt agaggaagac aaccgatcca gtctgatttg ttgctttttc
    20941 tttttactgg acactttttc caacttttcc gcttgttttt tcttttcttg gatcgatttt
    21001 tccaatcgtt ttttctcttt catgatcgac tctgcttctc gtagctgagt ctttttctcc
    21061 aattcctttt gagtgagata ggcactataa ttccccgaaa actctcggat ttctccgtct
    21121 tttacttccc agattttcga tgctagctca tctagaaaat agcgatcatg actaacaaaa
    21181 accagcgtac cataataata acgaagctct tcaatcagtc gatcaatact ttttctatct
    21241 agatgggttg ttggctcgtc tagaagcatc ccttgttcat aagaagaaag aagacgtatc
    21301 aactcgatct ttcgttcttc ccctccgctc catttccgct ctgaagtttc ttccagtctt
    21361 aactggaaat gactggctaa ttccaaattt aggtcctttg cttcagcgag ataggttaat
    21421 tgaggcaggt agttgaaggt gatctctgtt tggattttcc catgatcggg aaaaagttca
    21481 ccggctatta gtttgagcaa agtcgattta ccgacaccat tttctcctat gatccctata
    21541 cgatcttgct gatagacacg aagagaatca atagatagtt ctagctggtt gccaaagctg
    21601 acttgtagat ttgttatatt tactgctaaa ttttccatat gttttacact cctatttcat
    21661 gtaaaacaac aattatcgat actaaaaaag agcagaaaaa tctgtttgca aaaaaactgc
    21721 atcacatctt ttcctactct gaacgaaata actatgacta aatcaccaaa tctgtcatag
    21781 ctttcaccta ttctactgtc gttcgaaaaa atggacatac tccatccatg aaattgttgt
    21841 ttatacatgt cttttgaatt tatttagttg ttattcaaaa gttcgaaacg taatttcatc
    21901 gatgcagtca tttttccgac ctcctttttt attaggttac cacacacgat tcaaaaaaag
    21961 caatagctac tgaatagtaa ctgaaaacct tttattcgaa catagtgatc ttttctggtg
    22021 ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc tattgataaa ataataactg
    22081 gaacataacg gtctttatcc aaaccaaatg cctctgccaa ctgatctgct tcgaatccac
    22141 caattggatt tgtttcatag ccatgatcac gcgcaactaa catgaattgc atagccgcta
    22201 agctagaatc gactttgact acatcgttca tttcttctct tgaaaaactc ttgtaataag
    22261 gaatgatcgc agctaattgt tgatctctga cttcttttgg catcttccct gattcgtaag
    22321 cttggttgta gatttcttcg ccatattcgt aacattgcat atcgccaaaa atcatgacca
    22381 ttgcagaaga agtatcattt tgtctcgtat tgaaacggat caaaggacgc aagatttctt
    22441 ttgcttcctc gctttcagcg accacaaagc gccatggttt catattgaca gaagaaggtg
    22501 cagtcgtcgc ttctttgatc atttccagca tttcttcgtg tgggattttg tatgtttcat
    22561 catatacgcg aacggatctt ctaccagtca taattgttga aaaatcattt ttttgttcca
    22621 ttattattaa cctctttcgt ttctgttgtt atattgcaaa gttgggtatt caatttatct
    22681 aaaagtgtta gaaattgttt catttctaca tcgctgaaag ccggggatag cacggaacct
    22741 gtatctttat ggtccatttc acaatgcttg attttcttct gggctttttc tgttagcgct
    22801 acatacactt cccggttgtt ctgaggattt ctctgacgcg aaacatagcc atttgcttcc
    22861 aataccttca gatgacgagt aactgctcct tgatcaatct gcaacttttc ttgcaaactg
    22921 ctttgtagca atggattttc ttctttcagt ctcatcatga tctcgtatct agtaagactt
    22981 ataccgatat tttcttcaaa gatctggacg attttttgat cggctaattt gatttgatat
    23041 aaaaggtgac taatctcttc taaagacata atttcctcct ttctaataat tgactcgtca
    23101 acgattgact tgtcaataat agaataaaaa aagaaaaaag tcaaagaatc agctgtgtaa
    23161 taaaaaaaac gctctcaata gaaaagtata gagaaaagaa attttttgta gaatgaacgg
    23221 aaaaaatgat aaagaaagca caacgctaaa accaagatta ttgattctta tcaaactata
    23281 gaagatagct cttctgcagt agataatttt acaccgccaa aaatttccga aatcgccttt
    23341 tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg cttaaacagt aataaaaaag
    23401 ttatgacaag atgttgtcac aacctttttt gagtaaacgg tgttcaaaaa cttgcatctg
    23461 cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt ttttgtttgg ctgttcttac
    23521 tacttgatgc agggcagctt tttcactacc tctttttaat aaccataaaa tcctattcct
    23581 tcatatctcc agcccatcgg tgttagccaa tctctttcct tagcactcaa ggtatagtga
    23641 tgggtaccag ttttttcatt tggattaaat agtcgataaa ttggcttgga accaccagaa
    23701 taaaaactta gaccttcata acgccaaccg atttttgtta aagagttgat ctcagattgt
    23761 gccattgtgt aatgatggtc tccactgtaa ggattgtata aacggtagac aggttgtccg
    23821 ctgtttggtg cttgccaagc aatcccttca tattcccagc ctacagttgg cagataattt
    23881 ttttctccgg cattcaatgt atataaatgt tctcctgtat gacggttata cagacgatac
    23941 atcggagaag ttgaagttgg tgctgtatca tactgtgtca gattataggt ttcgattaca
    24001 ttgttcaatg tagaaccata attaggcgca gtggcataac gacctgttag ccaagccgta
    24061 gcatctttgt aagagttcgt attgcttttc catgcaccgg aatagtagta aacgcctggc
    24121 tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca atgattcttt gtacgaagga
    24181 tattttctaa aagcggcatt caccgtaatc cattgcccat tcacatattc cgaagtaggc
    24241 atcgtaacag attggccatt atagcttcct ttaatcccaa atagattgta attaggggca
    24301 gaagccaaag tactgttccc ccagccactt tcaacgatgg cttgagcaat catgactgat
    24361 gcatataagt cattggcact tgccaaactt tgtgcagttg aggcaatact atcaatgaac
    24421 gcttgttgag aattagcaga tgagcgagca taaaaagcat agccagtata gttttcgaat
    24481 tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc catctgtcaa ttggttcgtg
    24541 atttcttctg cattctctgg attgttctga gcttctgctg atgtactgtc ttcggtttgt
    24601 gtttgttctg ttgtttcagt tgttggttct tcggttgaaa ttgtattttc tgagacagta
    24661 gctgactgca aagtactttc tgtatcgttt tcaattcctg tttctgaaga actcgatgtc
    24721 tgagttgcga cagtttctgt cgattcttca gctagcactg ttgtgttggc aactgctaga
    24781 tttcctaaaa gaataacgct aaataataaa ttctttgctt tcatcttttt tctcctttga
    24841 attatgaaac ttttcctaaa taacagtgta actaaaaaag taaaagcaag tcaatgtcca
    24901 tatttaaata acatttgttt ctttttaata aatttgcttt ttctgcataa aaagacgtaa
    24961 atgaagaata ttcttctcca ttcacgcctt aaattgatgg tcgtttgaat aactatttaa
    25021 caagtaattc tctctggata ttatccgtta tggattatac ttcctgtgtc gtcggacgga
    25081 taatcatgtt gctgacagac atccggtctg gcgtttctat cgcaaatgct acagcagaag
    25141 ctatatcttc agatgttaat ccccattctt tttgcgcttc atgaagttca agagcagcct
    25201 ttttgtcgct gatggtttga tataattcag tttgaacagc tcctggagag atgatcgtag
    25261 atttgatgtt attttgccgt tgctcttgac gtaatccttc catgattgca cggacagcaa
    25321 attttgttcc gcaataaacg gcggaatcag gataaacaac atgtcccgcc acagaatcag
    25381 ttgaaatgat atgcccagat ttttgttttt ccatgattgg aagtacggcg gaaattccat
    25441 ttaacacacc catgatgttg atatcaagca tattttgcca ttcatcacgg tgaccttcaa
    25501 ctaatggagc ggttggcatg attcctgcat tattgtacaa tacatcgatt cgaccgaact
    25561 tttccatcgc taaatcgata actgcttgga cttgtgcaaa atctcttaca tctgctgttt
    25621 gaatatatag ctcagcttct ggtaaagatt cttttattgc ttttaggcga tcttctcggc
    25681 gtgcagctat aactagttta gccccttttt ctgctaatag cttcgttgtt gcttcaccga
    25741 ttccgcttga agcgcccatg atgacaatca ctttttctga taaatcattc attctattct
    25801 cctcctgttt tgaaaaatct cttgcttata ctcactatct cttatttcat cttactgttc
    25861 aaataatccg ttcgacaggt ttgacgattt tggcagggat tcctgcaacg atggtattgt
    25921 ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat ggcattctcg ccaatcgtta
    25981 cgcctggcag aatagttgct cctgctccga tccaagcatt tttcttgatg tggattggct
    26041 ttactgacaa gcctcttcgt tcttttggat cggttagatg gttcaccgtg atgaggttga
    26101 cacgaggacc aatcaatact gaatcttcta aacagatgcc acctaagtca acgaacatca
    26161 cttgacgatt gataaagaca tttttcccta tactaatatg tttgccgaaa tctgtataaa
    26221 aaggcagcag aacagtcacc gtatcatcta ctttgtcact aatgatattt tctagccgtt
    26281 tgcggatatc tgctggttca tggtacccag tattcagcgc tgctaaaggc tcttcgtttt
    26341 ccgcttggat ctgatggatc acttcaaaga gcggatccgt tactcgaatc tctttattga
    26401 cgatttgttt ttcgatgagg cttgtcattt tgctccctcc ttttatcctt actttataac
    26461 ggacggctta tgatgtctaa tacttataaa acatacttaa ctattccttt taactatact
    26521 tagtggtatg cttttttaaa aacaggaggg aaaaaatgga tattcgtata ctgacgtatt
    26581 ttttagcagt agctagagaa aaaaatatca gccgagcagc aaaatctctg catatcacgc
    26641 aacctactct ctcaaaacaa ctgaaagatt tagaagaaga acttggtgtg acacttttca
    26701 ctcgaggctc ccgagagata cagttgacgg attctggtcg atatctttat tcaaaaggaa
    26761 aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt aacaaatgac gcaatcatct
    26821 ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat ctctttcatt tctgatagcc
    26881 ttcattcgtt gatcaaggcg catcctgata tccagtttca tttatatagc gggaatgcag
    26941 atgatatcat agacaaactg gatagcggat tacttgattt tggactcgta atcgaaccga
    27001 caaacaaaca aaaataccag tcatttcgac tgccagccca agatcgttgg ggacttttga
    27061 tacatcggaa tcatccttta gcaaagcaaa cagtcatcca tccgcaggaa ctgaaaactg
    27121 ttcctttgat catttcccgg caaacgtcag tggacagtca actagctgaa tggctaggtt
    27181 tcagcataga tcagttatcg atcgtcggaa catataactt attgtacaat gcttctttga
    27241 tggtcgataa cgggactttt ggtgcattag ctctagatgg gatcatcaat acttatggga
    27301 cggaaaaagt attcatccca ctcgatccgc cattacaagc ttcactgaat ctcatttgga
    27361 aaaaagacca gcctctttct cctgctgctc gtaaattttt agatgtccta acggagaaat
    27421 acagttaagt agagtttttt tccttcgctt tcagcttttt aacaacgaat aagttaagca
    27481 aaagatccaa tgattaaata acataagttt cttaatttgt tccttccttt tatttctgat
//
LOCUS       DAZKMB010000024        27029 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000024 DAZKMB010000000
VERSION     DAZKMB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.024"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..1073)
                     /locus_tag="ACG8V3_000957"
     CDS             complement(105..1073)
                     /locus_tag="ACG8V3_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0299512.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1305..2138
                     /locus_tag="ACG8V3_000958"
     CDS             1305..2138
                     /locus_tag="ACG8V3_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0299513.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2172..3011
                     /locus_tag="ACG8V3_000959"
     CDS             2172..3011
                     /locus_tag="ACG8V3_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0299514.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3037..3597
                     /locus_tag="ACG8V3_000960"
     CDS             3037..3597
                     /locus_tag="ACG8V3_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0299515.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3796..5517
                     /locus_tag="ACG8V3_000961"
     CDS             3796..5517
                     /locus_tag="ACG8V3_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0299516.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5682..6824
                     /locus_tag="ACG8V3_000962"
     CDS             5682..6824
                     /locus_tag="ACG8V3_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0299517.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6840..8051
                     /gene="thiI"
                     /locus_tag="ACG8V3_000963"
     CDS             6840..8051
                     /gene="thiI"
                     /locus_tag="ACG8V3_000963"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0299518.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            8624..9271
                     /locus_tag="ACG8V3_000964"
     CDS             8624..9271
                     /locus_tag="ACG8V3_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0299519.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    9400..9616
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9664..12309
                     /locus_tag="ACG8V3_000965"
     CDS             9664..12309
                     /locus_tag="ACG8V3_000965"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0299520.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            12619..13932
                     /locus_tag="ACG8V3_000966"
     CDS             12619..13932
                     /locus_tag="ACG8V3_000966"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0299521.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            13922..14575
                     /locus_tag="ACG8V3_000967"
     CDS             13922..14575
                     /locus_tag="ACG8V3_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0299522.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            14630..15322
                     /gene="radC"
                     /locus_tag="ACG8V3_000968"
     CDS             14630..15322
                     /gene="radC"
                     /locus_tag="ACG8V3_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0299523.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            15472..15672
                     /locus_tag="ACG8V3_000969"
     CDS             15472..15672
                     /locus_tag="ACG8V3_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0299524.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(15926..17605)
                     /locus_tag="ACG8V3_000970"
     CDS             complement(15926..17605)
                     /locus_tag="ACG8V3_000970"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0299525.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(17607..17819)
                     /locus_tag="ACG8V3_000971"
     CDS             complement(17607..17819)
                     /locus_tag="ACG8V3_000971"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0299526.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            18212..19942
                     /gene="efrA"
                     /locus_tag="ACG8V3_000972"
     CDS             18212..19942
                     /gene="efrA"
                     /locus_tag="ACG8V3_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0299527.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            19939..21699
                     /gene="efrB"
                     /locus_tag="ACG8V3_000973"
     CDS             19939..21699
                     /gene="efrB"
                     /locus_tag="ACG8V3_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0299528.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            21794..21991
                     /locus_tag="ACG8V3_000974"
     CDS             21794..21991
                     /locus_tag="ACG8V3_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299529.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            22144..22629
                     /locus_tag="ACG8V3_000975"
     CDS             22144..22629
                     /locus_tag="ACG8V3_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0299530.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(22733..23326)
                     /gene="clpP"
                     /locus_tag="ACG8V3_000976"
     CDS             complement(22733..23326)
                     /gene="clpP"
                     /locus_tag="ACG8V3_000976"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0299531.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            23506..24033
                     /locus_tag="ACG8V3_000977"
     CDS             23506..24033
                     /locus_tag="ACG8V3_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299532.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            24124..24861
                     /locus_tag="ACG8V3_000978"
     CDS             24124..24861
                     /locus_tag="ACG8V3_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0299533.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            24865..25782
                     /gene="miaA"
                     /locus_tag="ACG8V3_000979"
     CDS             24865..25782
                     /gene="miaA"
                     /locus_tag="ACG8V3_000979"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0299534.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            25784..27013
                     /gene="hflX"
                     /locus_tag="ACG8V3_000980"
     CDS             25784..27013
                     /gene="hflX"
                     /locus_tag="ACG8V3_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0299535.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8907 a         4678 c         5785 g         7659 t
ORIGIN      
        1 acgaaaattg tttctcaaat ttttgtaaat tttcgactta gttttcgaag aagctatttt
       61 tgtcacagcc tctcttttta agtttagcta ttagataatg atgtttagtt ttggataaac
      121 gaaatcaatt cttcacccga caaaccatag tttacagcaa tcaatagttt taaacgtgct
      181 ttttgactat taatactgtt acagaaaata atccccattt tttctagttc ttttcccccg
      241 ccttcataat cataaacagg ctctgcgatt ccattgaaac aacgagaaac aagtactaca
      301 gggatttttt ggacaagaag cttttccaaa gcagctaagg cttgtggagg gagattcccc
      361 gcacctaaag cttcgattac caaaccgtct actttcgtat gggcgatggc ttccaataag
      421 tccccttgca tcccagcata tgctttaacg atcgggatag tcccatctac agcggaaatg
      481 tccagtcgct ttgtttctaa aagttcctgc aagaataaaa ttcgattctt agttactaag
      541 ccgatgggac ccaatgtagg tgttcggaaa gtagcaacat ttgtggtatg ggtctttgtc
      601 acgtagcgtg cagaatggat ctcatcgttc atgaccacca aaaccccctt atctagcgct
      661 tcttcacaac ttgcgacacg aatcgcactt tcaaaattat aaaggccgtc acttccaagt
      721 tcattacttg aacgcattgc cccagtcaaa acaattggta atttgttgcc aatcgttgta
      781 tcaagaaaaa aagcagtttc ttctaatgta tctgtaccgt gtgtgatcac cactccggat
      841 atacctgatg cataagcgga caagatacgt tcttttaata ccaacatgtg agaaggcatg
      901 atgtgtggtg agggaagatt gaagatatct tcaactaata attctacatg cttggggatt
      961 gtgatttctg catctaataa tgggtttgcc acatcaggac taacaccgcc cgtgagcatg
     1021 ttttctttca tggcgatcgt tccgccggtg tgtagtacaa gtattttttt cattttacag
     1081 acttccttac gaataatttt gcatagccgt taaatcttat tgtatactat ataaaagagt
     1141 tttgctatca gtgaagaata acgaatagcc taacttatca taaaaaagct gaccttatta
     1201 aaatggaaaa gactcaacct tgacctccta atacaatgga gtatctggtt gagtttcttt
     1261 tacaagaaag cagccaagga aatcaatcac gaaggtggcc ggaaatgatc agaatcggat
     1321 taacatcgtt taaagaacat gacaaattaa ctggtaaaaa gaataacact ctttatgaat
     1381 atgcagggtt tctaccctta gtggaattag atactgccta ttatggaatt ccccgaaagt
     1441 cctcagttga gaattggata agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct
     1501 atcaaggaat cagcggtcaa ggagactgga agccttatta tcagaccgaa gaagagatga
     1561 ttgcgcattt tttagatgtt atgtcgccaa tggtagatgc tggaaagatg ttttgctttt
     1621 tagtccaatt cccctcatca ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac
     1681 ttagaaagtg gtttgagggt tatcctgttt ctatagaatt gcgggattat tcttggtaca
     1741 gcgatgaatt tcttgaacgt acaagaaact ttatgtctag agccgctttt tctttagcca
     1801 tcatcgatga gccacagttg ctgaatacaa cgattccttt tgatccattt gtcaccaatc
     1861 cgaactttgc tctttttcga ttccatggta ggaatgcgca aggatggcaa gcaaatgata
     1921 aagagtggcg taaaaaaaga acgttatatc gttattcaga aaaagaactg caagaactag
     1981 cacatgaagt caaagtagtt gattctaaga caaaagaggt aggtgtgatt tttaataata
     2041 attctggtgg tgatgcggct gaaaatgcat tagcactgca aaaaatatta aatatcaagc
     2101 cagatggact caatccaacc caaatggatc tgttttaagg gcttatttta ttcaagaaag
     2161 gggcgaagtc tatgatcaaa gctatttttt ttgatattga cggaacattg gtcaccaatc
     2221 gttcaaaagc gttggaatca acaaaacaag caattgaata tgctcggaaa aacggcattt
     2281 tatgcggtgt agcaacgggg cgcagcccag taaagatcaa agaaatcatt gatgaattgg
     2341 aattagatat gtatgtcgtg tataatggtc aactggtttt tactgcagat cgcacgatta
     2401 tcgaccaccc ttttgaacaa aaagtattgg aacatattgt tgaatttgca gatgagaacc
     2461 atcgacaaat cgtttttggt gcgagaaatc ggttagatgg atcaacgacg atgctattag
     2521 gacaatcgat ttttatcaag cgactagtca gctttttacc gcgtaaattc ccggtacgtc
     2581 tgatgaaaaa aatcctccag gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt
     2641 tagagatatt aaaagaacca atttatcaat gtatattgct cagtccggag tcagaacaga
     2701 aaaagttaga acatcgcttt cccgagtgta cgtttcagcg ttcaaatacg tatactgtag
     2761 atatcatccc aaaaggtggt tcaaaattac taggcatcca agctttcgca aatgctgtcg
     2821 aaattgaaat ggaagaaatt atggcattcg gcgatcatta caatgatatc gagatgctga
     2881 aaggtgtagg aattggagtg gccatgggga atgctcagat cgaagtaaaa catgctgcag
     2941 actttgtgac tcaatcaaat gaagaagatg ggatatacga aggactgaaa cacttcggtc
     3001 tgatttatta gtcaatgaaa gaaggaaaaa atcagtatgg aagcagagac accttttcaa
     3061 aaagtagaaa cattccacaa aacttttgat ccaagaagac cagagatacc cacggctttt
     3121 acagaaaatg aagctcatac acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt
     3181 gcagctactt cacctaataa agaacaactg catgaagctg ttatgctgct gcacgaagca
     3241 ttagataaag cagaagaaaa aatacttggt aaaacacagt ggggaagtga tccgctggtt
     3301 gaacaagtag atgcactgac agatatcctt tactttactt atggttcttt ttcattacta
     3361 ggaatagtgc ctgatcgaat attcgaaatc gtccatcagg caaatatggg gaaattattt
     3421 ccagatggaa aacctcatta tgaccctgta acaaataaag tgttgaaacc tgaaaactgg
     3481 cagatggaat atgctccaga acctaaaatc gcggcagaaa tccaacgtca gattcaagaa
     3541 gcgattgcta agaagaaatc cgctgatata ggcatgtgca caaatgaaaa agagtgatca
     3601 gagtttcact atttttgtta tctttatacg atggaaaaac aaaagatttt cgtttaagtt
     3661 agtgaatgca tgaaaatagt atgctataat ctagaataga tgtcttttgc acgagagacg
     3721 tattatttgg atttatgcca agcagaacgc catctgttgc ataaaagatg catactgaaa
     3781 acggaggaac cataaatggg aaataatgtt atcatcggaa tcgtcattgc aatcattata
     3841 gtcgccattg tcgtcttttt ggttagttac ttgatgaaga aaaaaaacca agatcgattg
     3901 gatgccttag aaaaacgcaa ggaagaatta tttgacttgc cagtgattga agaagtcgat
     3961 gatgttaaaa aaatgcatct ggttgggcaa agccaaaata cctttcgtga atggagtcaa
     4021 aaatggaatg acatatctac ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg
     4081 gaaaacctca atgaaagttt tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa
     4141 gctacaatgg accaaatgga gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg
     4201 cgggaaacgg aagaacgcaa ctcactagct gttcagcagg cacttgacgt ctatgaagaa
     4261 ctgaaagaaa aagtcagaga agacgagcat gaatacggac cagctctttt tgaattgcaa
     4321 aaacagatca aagtgatcga aaaagaattt acgcagtttg tgactttaaa cacttctgga
     4381 gaccctgttg aagcccgcga ggttttggat aaagcagaaa aacatacgta tgaagtggaa
     4441 gctctgatga aaaaaattcc accgctgtat gaggagctcc ataccacttt ccctgaacaa
     4501 cttgaagaaa tcagtgatac ttacgagaaa ttgatggaag aacaatatgt attcccagaa
     4561 gaagatcttg ctgaagatat cgcaaaagta agtcgaagaa ttgaaaattc attagccaat
     4621 cttgaaaaaa cagaagttga aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt
     4681 gattcgttat atgatatctt agaacgggaa atggaagctc agagatatgt aaaaactaac
     4741 caatcaacga ttgcagagta tatcaaacat acaacaaaga ataaccgtca attattgatt
     4801 gagctggatc acacatctca aagctataca ttgaaccaca atgaaattgg ccgtgtacga
     4861 ggtttccaaa ccgaagtgga agaaatggaa cgtcaaaatg aacagatgat tccacagatc
     4921 cgtcaacacg aaattcctta ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc
     4981 ttagaagata tcgaaaagca acaagtagag atcgacgact cattacatga attgagaaaa
     5041 ggcgaaaaag aagcgcaaga aaagatcgat acattcgaat tcaaattgcg cagtttgaaa
     5101 cgttttgtag aaaaacaacg tttgcctggc ttgccgaatg attacctaga attctttttt
     5161 gttgctactg accgtattga agaactaagt gttgtcttga ataaaattcg agtaaacatg
     5221 gaagaggtca atcgtttagt tgctctatgt gaagaagatc tagaattatt agataagaag
     5281 acacatgatt tagtagatgc agcagcatta acggagcaaa tgctccaata tgcaaatcgt
     5341 tatcgtcata cacacgaaga agtgagagaa gcaattgata aaagctacta tttattcaat
     5401 aaagaatatc actatcaaga agcattagac gaaatcggta cagcattaga gcgagtagaa
     5461 cctggtgctt tcaaacgcat cgaagatttt tattttaatc atccagactt agtataaagc
     5521 aaaaaagtta gtaaaccagc aagagcaaga cggattattg tcttgttctt gtcttttttt
     5581 gtatatttga aaattgaaac aagtgaaaat ttcacctgct cttgaaagca ccttggacaa
     5641 ccgttataat agaaaagaat ttcaaaaaag aggggtttct tatgatatat tttgataaca
     5701 gtgcaacaac cccaatcttt ccgcaaagct tagatgcata tgtaaaaacc agtcagcgga
     5761 ttattgggaa tccatcaagc ttgcatgatt taggcaatca agctaaccgg ttgttgcagc
     5821 aagcaagaaa gcagatcgct gatttgatcc atgtagaacc agaagaaatc tattttactt
     5881 caggcggaac agaaggagat aactgggtat taaaaggaac gatgatcgaa aaaagagaat
     5941 atgggaatca tatgatcatt tcaggagtcg aacatccggc cgtcagcgag acagcagaac
     6001 aattaaaaga attaggaatc gaagtaagta tcgccccagt ggataaacgc ggatttgtac
     6061 gtgtcgacga attgaaagaa ttaataagaa aagagacagt attagtctct gttatggctg
     6121 tgaataatga agtaggatcg attcagccca tccaggaaat cagtgatctt ttggatgatt
     6181 ttcctaaaat tcatttccat gtagatgctg ttcaggcgat tggaaaagta ccattctcgc
     6241 aatggttgac agaaagagtt gattttgcaa cattttcagc tcataaattc catggcccac
     6301 ggggaactgg tttcatttat tggaaaaagg gtcgtcgttt agcaccatta ctgacaggtg
     6361 gagggcaaga aagaaatcag cgaagtggga cagaaaatgt tccaggtatt gtggctatgg
     6421 ttaaagcttt acgtttatca ttagagataa aggaacaaaa acctgagcag acacaacgct
     6481 taaaagtagc tctaatcaat gcgttgaaga cgtatgagaa agtgacgatt ttttcagaag
     6541 gaactgacta tgcaccgcat attttatgtt ttgctctaaa aggaattcgc ggcgaagttc
     6601 tcgtccatgc cttagaagaa aaacaaatat ttgtttctac tactagtgcc tgctcctcac
     6661 gtaaacatat ggcaagcagt acattgcacg caatgcatgt accaaatgat ctggctacat
     6721 ctgctgttcg aatcagctta gatgaaaaca attcgcttgc agaagtcgag caatttatga
     6781 ttattttcaa gcagctttat aagaaatttt ctgcaatcaa ttaaaagagg agtgttcttg
     6841 tgaaatacag tgaaattatg gtccgttatg gggaactttc gacaaaagga aagaatcgaa
     6901 aaagctttat catgcaacta gcacaaaatg tcagacaagc tttgaaagac tttccagaaa
     6961 tcaaaatcca tgctgatcgg gaccggatgc atcttttatt gaatggcgca gacgggcagc
     7021 tagttattcc aaaactagca aaaatatttg ggatccaaaa tttctctcca agtattcgtg
     7081 tagaaaaaga tgtcgaagtt ttgaaacaag ctgttcaaga catcatgaaa gaaatttata
     7141 ctgggaaaga aacatttaaa attatcgcta aaagaagcga tcaccaattt gaattgacaa
     7201 gtaatgaatt gaatcaaaca ttaggtaatg ctgtttttga tattttcccg catatccaag
     7261 ttcaaatgcg tcaaccagat attccattac gtgtagagat tcggcgtgat ggcgcttatc
     7321 tatcgtatga aacgatcaag ggagctggag gattgcccgt aggaacaagc ggaaaaggaa
     7381 tgcttatgct ttctgggggg attgattctc ctgtagcagg gtactttgcc atgaagcggg
     7441 gagtggaaat tgaagctgtt cattttgcta gtccgccgta tacaagtgaa caagcactgc
     7501 aaaaagcaaa agatctaaca gccaaactag caccttatgt gggaacgatc cattttatcg
     7561 aagtgccatt taccgagatt caagaagaaa tcaaaaagtc agtgccacaa ggatattgga
     7621 tgacaattac tcgtcgaatg atgttgcgtt tgaccgatga aattcgccgt attcgtcatg
     7681 gtttagtcat tttgaatggt gaatcattag gtcaagtggc ttcacaaact ttgcaaagta
     7741 tggtggctat caatgaagta acgaatacgc caatcattcg gcctgtagcg acaatggata
     7801 aacttgaaat cattgaagta gcagaacaga tcgatacatt taatctttct atccagcctt
     7861 ttgaagattg ctgtacgatt tttgctcctc cacagccaaa aacacgccct caactggaaa
     7921 aggtccatca atatgaagag cgtttagcaa tagatgaaat gatccaacga gcactagctg
     7981 gcttgaagat ggaagagata caagcagaat cgtctaatga aaaaactgaa gaattttcag
     8041 aattgctata agaggttatg aaaatagtgt tttgaccaga gtattagaga aacaaaacga
     8101 aaaaatagct ccatgtattt taccgttttt gaactaatgt cgaagattag ctgattgaat
     8161 accatttatc aagaagttat ggaaaagccg gttctttttt actaactgaa aaataaaatt
     8221 ttatttagag atcaagctac agttgataaa tgatataaaa aagagaattt cccatgaaat
     8281 agcgaggttg tgacagaagt gggcagcttc aagaaataag aaggaattta cgaaaattgc
     8341 ttttcaaatt tttgtgaaat ttcggcttat ttttcgaaga agttacttct ggaacaccgt
     8401 gtatcagagg ttgcgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat
     8461 agttcctcat attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc
     8521 gttttatttg tctcacaccc ttttccttcc tacttgtaac cttgctcccg tgttagaaac
     8581 atgttacact aaatatgtga aattattaac aaggagcgtc gttatgcgag aaaaaacaga
     8641 tacactgcca aaagcaactg cgaaaagatt acctttatat ttacgctact tgaagatgct
     8701 ggatgactca ggtataagta gaatcaaatc aaatgagttc agcgaaatca cccaaattcc
     8761 ttctgcaacg atccgcagag acttctcgca attaggcgag ctaggcagaa gcggatatgg
     8821 ctatgatgtc ccttttctta tcgatgtatt caataatatc cttaatacta aagaagaaaa
     8881 acgaatcgca ttagtaggat atggaaatct tggcaaagca ttgaaacaca acaattttag
     8941 aagaaatgaa aatctaaata tcgtgtgcgt ctttgataat gatccagcac tgatcaatcg
     9001 agtaattgat ggagaaatga tctatccgat tgatcgtttc gcggagatag ccaaagcaaa
     9061 aaatgtgaca gtagcaatct ctacggtacc aagtaaatat tcacaatctg ctattgatga
     9121 aattgtcaaa ggaaatgtaa ccgctatact taattttgcg cctgacagag taactgtccc
     9181 agcttatgtc aatgttcaat acattgattt gacaaccgag ttgcagacgc ttatttactt
     9241 tgatgagaat tactcagaag ttttttctta ggaaaatttg agtaggctta ttgatcttgc
     9301 gagaactcgt cgtaattctt tcaaaaattt tttagtacta aatgaagtgg ttttttcacg
     9361 accacttgtt gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct gaacaagagg
     9421 agtaactggc acattcattc ttagagagag aactatctgc tgaaaggttc tcaatgacaa
     9481 ccagtgaagg tagcttggga gtttctgtct tgaatggagt agagatagac gagtcgtgat
     9541 cgttaactca cttgagaggc aataatgatt gattattgca atttaggtgg taccgcgtaa
     9601 atcatacgtc ctagaatacg aaagtatttt aggacgtttt ttagtttaaa ggaggaaaac
     9661 agcatgacgg aagaaaataa cctatcaaca aaatataatc cgcaagaagt tgaagctgga
     9721 cgttatcagc attggttaga acaagatttg tttaaaccga gcggagataa aaaagcaaaa
     9781 ccttattcaa tcgtaatccc gcccccaaat gttactggaa aacttcattt agggcatgct
     9841 tgggatacga ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg atatgatact
     9901 ttatggctgc ctggtatgga ccatgcagga atcgcgacac aagcaaaagt agaagcgaag
     9961 cttagagagc aagggatttc tcgttatgat ctaggtcgtg aaaagttcat cgatcaagtg
    10021 tgggaatgga aagaagagta tgcgagccac atccgcgaac agtgggctaa aatgggctta
    10081 tctttagatt acagtaggga acgttttaca ttagatgatg gattatctga agcagtacgt
    10141 aaagtttttg ttaccttata cgaaaaaggt ttgatttatc gtggtgaata tatcatcaac
    10201 tgggatcctc aagcacgaac agctctatct gatatcgaag ttattcataa agatatcgaa
    10261 ggcgcctttt atcatatgag ctatgaacta gcagatggat caggcgtagt agaaattgcg
    10321 acaacccgtc cagaaacgat gttgggagat acggcgattg ctgttcatcc agaagacgag
    10381 cgttatcaag cgctcatcgg caaaaaagtg attctacctt tagtcaataa acaaatccca
    10441 atcattgcag atgaatatgt agatatggaa ttcgggacag gtgtagtaaa aatcactcca
    10501 gcacatgatc caaacgactt tgaggtaggt aatcgtcatg atcttccacg cgtaaatgtt
    10561 atggatgatg atggtacgat gaatgaacta gcaggaaaat atgctggaat ggaacgtttt
    10621 gctgctcgta aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat taaaatcgat
    10681 acgatgatcc acagtgtggg tcattccgag cggacaggtg tcgttgttga acctcggctg
    10741 tctactcaat ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga taaccaagaa
    10801 acagatcaag cagtggaatt cttccctcca agattcaatc aaacgttttt acgttggatg
    10861 gaaaatgtcc atgattgggt aatctcacga caattatggt ggggacatca aattcctgca
    10921 tggtatcaca aagaaacagg tgaaatgtat gtagggatgg aagcaccagc ggatagtgaa
    10981 aactgggtac aagatcctga tgttttagac acttggttta gttcagcttt gtggcctttc
    11041 tcaacaatgg gctggccaga tacggaaagc gaagattata agcgatactt ccctactagc
    11101 actttagtaa ccggttatga catcattttc ttctgggtaa gcagaatgat tttccaaagc
    11161 ttggaattta ctggcgaacg tccatttgag aatgtcttga ttcatggatt gattcgtgac
    11221 gaacaagggc ggaagatgag taaatcatta ggtaatggaa tcgatccgat ggaagtaatc
    11281 gaaaagtatg gagcagatgc tctccgctgg ttcttatcga atggttctgc accaggacaa
    11341 gatgtccgct tcagctatga aaaaatggat gcttcttgga actttatcaa caaaatctgg
    11401 aatgcttcac gtttcgttat catgaatatc gaaggaatga ccgtagaaga gatcgattta
    11461 tctggagaaa aatcagtggc tgaccggtgg atccttactc gtttgaatga aacgatcgaa
    11521 cgagtaactg agctatttga tcgttttgaa tttggtgaag caggtcgaca actgtataac
    11581 tttatctggg atgatttttg tgactggtac atcgaaatga gtaaagaagt actttatgga
    11641 gatgatgctg tttcaaaaca aaccacccgt agcatcttag tccatacact tgatcagatc
    11701 ctacgtttat tgcatccaat catgccattc gttacagaag aaatatggga acatattcct
    11761 catcagggaa attctctagt agtggcagaa tatcctgttg tacgtccaga gtttgatgat
    11821 gaaacagctt ctaaagggat ggaagtattg aaagaattga ttcgagccgt tcgaaatatt
    11881 cgttcagaag tgaatacacc attgtctaaa ccaattacgt tgctaattaa aacaaacgat
    11941 gctaaaatcg atcaattttt agttgaaaat acaagctata tcgaacgctt ctgtaatccc
    12001 gaagagttga cgatatcaag tgagatcact gcgccagatc tagcgatgtc agctgttctg
    12061 acaggtgcag aaatcttctt gccgttagca ggattgatca atattgaaga agaaatcaaa
    12121 cgattagaaa aagaactgga aaagtggaca agcgaagtaa aacgtgtcca aggaaaactt
    12181 tctaatgaac gttttgtttc taacgcgccg gaagaagtag tagaggcaga gcgtgcaaaa
    12241 gaaaaagatt atttagagaa gcaaacggct gtcaaagaac gaatcgaaag tttgcgtact
    12301 attcaataag aatagaaaaa gagagtgtga aatcagctag ctattactaa aagctagctg
    12361 attgaacatc gttacaggaa agagtttgag cctcactctt agcttcgatt cataaggaga
    12421 tgtgacataa attttgtcgc atctcttttt tagtctactc ttattatgta ggagaaggag
    12481 ggaggtggat catgtatcaa gagtcaataa agtagttgat aaaatcgttc ctctctctgt
    12541 ttcttttctc tttgtcatac ttttattttt cttatgtgtt ctatttgtta taataaaaga
    12601 gaaatggggg aattgttgat gacgattgaa gaagcaatag aatggatcca tagtcgatta
    12661 ccatttggtt cacgtcctgg ccttgaccga gtcgaagctt tattagaagt ggtagggcat
    12721 ccagagagaa aagtaaaaac gatccatatc gctggaacaa acggaaaagg ttctactgta
    12781 gcttatttga gaagcctatt agaagaggct gggctgacag taggtacatt tacttcacct
    12841 tatatcgaaa cgttcaatga acggatcgca atcaatggca aaagcattcc agacaatgat
    12901 ttgatccagc ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca atttgaagca
    12961 gtagctggta ttactgaatt cgagacatta acagcaatgg catttgatta ttttttgatg
    13021 aagaaagtgg acgtagcgat tatcgaagtt ggcttaggcg gcttattgga ttctaccaat
    13081 gtagctgtac caatgctgac gggaataaca acgattggac ttgatcatac cgaaatactt
    13141 ggagatacaa ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca aggcgttcca
    13201 gtagtaacag gaaatattgc cccagaagct tttactgtca tccaacaaac agctaaagca
    13261 aaaaatgcac cgctttatgt atacggggaa aattataaaa taaactatct gcatcctgat
    13321 gagatgtggg gagaagtatt tgaatttttt aatgaaatag gaaagatccc tcaattaacc
    13381 actccgcttt tagggcaaca tcaagctgaa aatgcaggag tagccatcga attgtatttt
    13441 gtttactgtc aattggaaaa gctgcctttc cgagaaaaag atgttttgaa gggattgaag
    13501 aaatctcgat ggccaggaag aatggagcgt ttgagtaacg agccattagt tgttttagat
    13561 ggtgcccata atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa agaattcaaa
    13621 ggatatcaaa tcaacatttt gttttccgcc ttacgaacta aaaatgttag agaaatgcta
    13681 gaacgattgc agcaagtacc aaatgtccat atttatttga cgacctttga acatccaaaa
    13741 gccatcgaat taagtgaagt atcagattta gcaagtgaga gagtatcaat cgtttcattg
    13801 tggcaattcg gattagcaga tatattggaa aagatgacaa atgaagacgt attagttgtg
    13861 accggttcac tttatttcat ttcagaagtt cgccatttat taatacaatt aggaggagat
    13921 catgaagtat aatgcagtga tttttgacat ggatggtttg ctgttcgaca cagagttagt
    13981 gtattacgaa gccacacaag tagtagcaga caagatgggt tttccttatg ataaagacct
    14041 gtatttaaaa ttcttaggtg tttcagatga agaagtatgg gcgaattatc atatgatttt
    14101 ctcggaattt ggcaaaacaa gtgtacaaag attcatagat gattcatacc aggaaacgct
    14161 tgaacgtttt tcgcaaggag cggtacaaat gaagcccggt gtgttggaac tcctctcttt
    14221 tttagaagaa catcagattc ccaaagttgt cgcttccagt aaccaacgag cagtcattga
    14281 acttttattg aagaagaaca atctgtttga acgatttgag acgattgttt ctgcagaaaa
    14341 tgttcaacga gcaaaaccag acccagaaat tttcttgtta gctaatcaga agctaggtac
    14401 agcgaagaaa gaaacattga ttttagaaga ttcacaaaat ggtattttag cagcttctgc
    14461 ggctgagatc ccggtagtca tggtaccaga tctgcttcca ccatcccaag aattgatcaa
    14521 aaaaacagct ggggttgttt cttctttaca tgaaatccct gcattgttaa aataaaaatc
    14581 catctctttt cgtatcttat tttagataag atagaaagga gatgtttttt tgggaaaacg
    14641 attgatcaaa gaagtaccta ctagctcttt accaagggaa cgtatggaaa tttatggcgc
    14701 ggaagcttta tcggatcagg agcttttggc aatcttactg cgtaccggtc aacaccctta
    14761 tagtgtgatg tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg cctctttacg
    14821 acaggctact ttgcatgagt tagaagagat tcgagggata ggacgcgtga aagcaatcga
    14881 aatcaaagcc ttgatcgaat taggtcgacg aattcagacg gatcatgaaa gagcggctcc
    14941 accggttcgt accagctacg aattggccca gcaattgatt ctggaaatga aagatcacaa
    15001 acaagaacat ttggtttgta tttatttgga cacgaagaat caagtgatct taaaaaaaac
    15061 agtgttcatc ggttcgctga atcagtcagt cgctcatcct agagaaattt tccattatgc
    15121 tgtacgttat tgtgcagcaa ggatcgtttt agcgcataat catccttctg gtaatgttat
    15181 tccatcgcag caggatatga attttaccaa aaggatacaa aagtgtggag aaatgatggg
    15241 gatcactgta ctggaccact tgatcatcgg acgcaaacga tattttagtt tgagagaaga
    15301 aggaatgatg gaagagaagt aagttgcttt cttgcaaaaa aaatgaagca agtgtacaat
    15361 tatattgtaa ttttgtttgg gaatagaagt tgaaacatct gttgcacctc tatcgtacac
    15421 gagcaatgtt acaatttaaa ttagtgcttt tggcacgagg aggatattat tatggaaaac
    15481 ggaacagtaa aatggttcaa cgctgaaaaa ggatttggat ttatctcacg tgaagatgga
    15541 agcgacgtat tcgttcactt ctcagctatc caaggtgacg gattcaaaac attagaagaa
    15601 ggtcaagcag tgactttcga cgttgaagaa tcagaccgcg gcccacaagc tgcaaacgtt
    15661 gtaaaagctt aattttatct gtaaatcaaa ttgatggtag acatcaattt ttacgagaga
    15721 acaaagcgag taacaaaaat acctgccaca agagacaatt tctcttgtgg caggtatttt
    15781 tttgtatgaa aaagtaaata aatttaagag gctatgaaag agccaccttg tatcaaggaa
    15841 taagaacaaa caaaaatggc ttctcatgtt tttcatttgt tttctcttat attcaggaca
    15901 aggtgctgat ttcataacct cttatttatt cttcatctgg tgtcattacc attggcaaaa
    15961 tcattggatg tctttctgta cgttcgaata agaaaggctg aagcgcgcca gtcatcgctt
    16021 ctttcaattt atattctgaa cagtcaggtc cattcaaaca ttctcttaaa gcgtggaata
    16081 agattcgctg agcttcattg atcatttctc ctgattctcg catgtagaca aatccacgag
    16141 agagaatatc tggaccagcc aagatttctt ttttcttgat atccactgtc gcgacagcta
    16201 atacaagacc ttcttctgaa agcaggtgac ggtcacgtaa aacgacatta ccaatatcac
    16261 cgatcccgct accatcgata tacacatctc cagccacaaa atgcccagca ggacgcgcag
    16321 aatcgctagt taacgctaaa acatcgccat tttccatgat gaagcagttt tctgcaggaa
    16381 cacctacatc ttgtgctaga gaagcatgta ctttcaacat gcggaattct ccgtggacag
    16441 gaataaaata ttttggcttc atcaaacgaa gcattaattt ttgttcttct tgtccaccgt
    16501 gaccggaagt atggatgtta ttgatttttc cgtgaatcac ttctgcacca gcttcagaca
    16561 gcaaattgat caaatgattg acacttgttg tattaccagg aataggtgaa cttgaaaaga
    16621 tcactgtatc gtctggctgg atttggattt gacggtgagt accattcgcg atgcggctca
    16681 acgcggccat cgcttctcct tgagaacctg tacataggat catcgtttca ttggcaggca
    16741 agcgattgat ttcgtgtgca tctacaaatg tgccgtctgg aactttgata tatcctaaac
    16801 gttgaccgtt gacgatggca ttttccatgc ttcgtccgaa aacagcgatt ttacgaccag
    16861 ttgccacagc ggcatctgct gcttgctgca aacggaagat gttagaagca aagctggcaa
    16921 aaatgatacg tccttcgatt tttgaaaaga tcttcaaaat agaatgccca atcgtttttt
    16981 ctgatttagt aaatgttgga atctcagcat ttgtactatc agataaaagg cacaagactc
    17041 cttcttcccc gatacgagcc attttatgca agtttgctgg ttcgccaaca ggagtgaaat
    17101 cgaatttgaa gtctccagtc gctacgatat ttccagaagg tgttttcacc acgatcccta
    17161 atgcatctgg gatactatgg gtcgttctga aaaagctgac agaagtcttt ctaaaacgga
    17221 tgatcgtatc ttcattgatc tcatggagct ctgcatctcg aagcaagcca tgctcatcta
    17281 atttgtttgt gatcaacgct aatgcgagag gaccagcata gataggaata tttgcttgtt
    17341 tcagcaagta gggtattccg ccgatgtggt cttcgtgccc gtgagtaatc accaaggctt
    17401 ttactttgtg taagttttgt atgatgtagc tgtagtcggg aatgacatag tcaattccaa
    17461 gtaaatcgtc ttccgggaat ttgatcccgg catcaatcaa aatgatttca tcttggaatt
    17521 gtaccccata cgtatttttc ccgatttcac ctaatccacc aatagcaaag acgcctgtct
    17581 cgttattttt gatggaaagt ttcatattaa aactccacta atgagaaatc agcattttct
    17641 ttttcgtact ctagatgact tccttctaga agttgaacga attcaatatt gtatggggta
    17701 ttttcttcca cacgttggcg agcgtctaca tcgctttttg cctcaatgta aagagatttt
    17761 gtttcttccc gtttagggtt gcgtactttt gtttcttgat aataaacttt atagatcatt
    17821 gttttgtctc ctttatctgc gtaaagcagt gatttagcta gttgattttt ttagacaaca
    17881 caaaacgagg ggacaagcca cctggttttt catatgcttt gactagaata ctggattcga
    17941 gtcaaagaga caagcctcgt ttttgcttat ctgttcggat attttttcaa ccgtaccttc
    18001 tgtataaaaa atatacaaca attaattgtt attctagcat aaaacagcaa ataagtatag
    18061 aaatcaacta gattgaaaga aaaaatcaat gtttatcatc ccgctataat cataatgact
    18121 tttttatgag agaagtgttc tattaatagg ctcgttgtgc tatcattttt agtaatgaaa
    18181 atgtaacaaa agattaaaag gagatgagaa agtgaaacta atgtggcatt ataccatgcg
    18241 ttataagaaa ttcttgtttt tcaatttcat atgcgtattt ggttttattt tgattgaatt
    18301 aggacttcct acgattttag cacgtatgat cgatgtagga atccgcaatg atgattttga
    18361 ttatgtgaag caacaaggga tatttatgat cgttattact gtaatcggta ttattttgaa
    18421 tatcttgtta ggttattttg gttcgaggat tacgactaat atcgtagcag atattcgaga
    18481 tgatttgttc gaaaagatcc aaagttattc gcatcaggaa tacgaaaatc taggcgtatc
    18541 ttcattgatc actcgtacaa caaacgatgc ttatcagatc atgttgttcc tgcaaaatat
    18601 tttacgtatc ggttttatgg caccaatgat gttcattgtc agtttgtaca tggtcatgcg
    18661 tacaagtgtg acactcagtt tgtatgtagt aggtgcatta cctttactat tattggcagt
    18721 cgtagcgatt gctaaggtgt ctgaaccgtt atctaaaaag caacaaaaaa acttagataa
    18781 aatcaacagt atcttgcggg aaaatctttc aggattgcgt gtgattcgtg cattcgtcaa
    18841 tgaaaagttt gaagaaaaac gttttgctgt agtcaatgag aattatgctt ctagttcaaa
    18901 aagcttattc cgtttgatgg ctgttgcaca accgggtttt tatttcttat ttaatatcgt
    18961 aatggtattg atcatctgga gcggggcagt gcaaatcgat gcaggcgagc ttcgtgtagg
    19021 agatttgatt gcgtttatcg agtatatatt ccatgccttg ttctcattca tgttatttgc
    19081 tagtgtgttc atgatgtatc caagagcagc tgtttcagct ggacgtatcc aagaagccct
    19141 agatgccaaa ccttcgattg tagaagatcc aaatggtata tctgatacaa aaactaaagg
    19201 gtatatcgaa tttcaaaatg taacatttgc ttatccaggc cattcgcaag agccagtgat
    19261 acgaaatgtc agttttacag cctctccagg agagaccgtt gcttttattg gaagtaccgg
    19321 tagcggaaaa tctacgttga tccaattgat ccctcgcttt tatgatgtga gtgaaggaaa
    19381 gatcttgatc gatggagtag acgtgagaga ttatcggcta agcaagttgc gagataagat
    19441 tgggtttatt ccgcaaaaag cgctgctatt tactggaacg attgcagata atttgcgata
    19501 cgggaaagaa gatgctacgc aagaagaaat ggaacgtgca gctgagattg ctcaagcttc
    19561 agaattcatt tctcgaaaac cagatggatt taatgaacat ttatcagaag gtggttccaa
    19621 tttttcaggt ggacaaaaac aacgattggc aatagccaga gccatcatac gccgtccgga
    19681 aatctatatt tttgatgaca gcttttctgc attagattat caaacggatg cgaagttacg
    19741 tgctcgtctg aaaaaagaaa cgacagaatc tactgtattg atcgtagcac aacgtgtggg
    19801 aacgatcatg catgcagata agatcatcgt attaaatgaa ggagaagtcg tcggtatggg
    19861 gacgcatcgg gagttgttgg aaaaatgccc gatttattat gatattgccg cttctcaatt
    19921 atcaaaggag gaattagcat gaaaaaaacc ttttcttcct taaaacgttt agctcgatat
    19981 atcagaccgt atcgtgagac ttttctttta gtgatcctat ttacagttct gacagtggct
    20041 ttcaacgtgg ctttgccata tgttacagga cttcctacta cagaaatcag ccgtaatatt
    20101 gccaatggcg aatcaatcaa tttttcttat atattcaaat gtctcatttg gatcacagtt
    20161 gttggaatag ggtattgtat ttcgcaatta ctttctggtg ttttgatgac aaatgtcgtt
    20221 caaagtgcca tgcatgattt gagaagagac atagaagaaa aaatcaatcg tcttccggtt
    20281 tcatatttcg ataaaaacca gcaaggaaat attttgtctc gtgtgacgaa tgatgtagat
    20341 gcagtcagcg gtgctttaca acaagctttt attggtatcg taaatgcgat attgggaatt
    20401 acgttggctg cagcaatgat gttttatatc cagccgatga tggcgctgat ctctatgatc
    20461 atgattccaa catcgatctg gatctcaaaa cgagtgatca atgcttcgca gaaatatttt
    20521 caaagcatgc agaattctct tggtgagcta aacggctacg tccaagaaaa tatgacaggt
    20581 tttagtgtat tgaaagtata tggacgcgaa aaagaaacat tggaaggatt caaaaaagtt
    20641 aatcacagct tgaaatactt tgggtttcgt gcagcattca tttctggctt gatgatgcct
    20701 ctagttcaat taactgccta tgctacttat attggtatgg ctgttctagg aagcttttat
    20761 gttattactg gggcgatcgt tgtcggtcaa ctacaagcat tcatccaata tatttggcaa
    20821 atcagtcagc caatggggaa cgtgacacaa ttgtcttcag ttttgcaaag tgcatcagcg
    20881 gcaacgaaac gtgtattcga gattttagat gagccagaag aaaaagaaaa caaagtagat
    20941 gcttcactgc cagaaacgat tttaggagat gtgacattcg atcatgtcga ttttgcatat
    21001 gatccgaaga aaccattaat ccaagatttg aattttgaag tgaaagctgg tcaaaccgta
    21061 gctgtggtcg gtccgaccgg tgccggcaag acgacattga tcaatctatt gatgcgtttt
    21121 tatgacgtag acaaaggagc aataaggatt gatggaatcg acaccaaatc aatgagtcgc
    21181 agtgatgtgc gttcgctatt tggtatggtc ttgcaagatg catggcttta tcaaggaacg
    21241 attgcagata atatccgctt cggaaagctt gatgcgacag attatgaagt agttgatgct
    21301 gcgaaaacag caaacgtaga tcattttatc cgaacgatgc cagatggata tgatatggcc
    21361 atcaattctg agggagacaa tgtgtcttta ggacaaaaac agttgctgac gattgcccga
    21421 gcagtggtct ccgatcctaa aatcttgatt ttagatgaag caacaagttc agtagacacc
    21481 cgtttagaag cgttgattca aaaagcaatg gatcgcgtga tggagggacg gacaagtttc
    21541 gtcatcgctc accgtttatc gacaatccgt gatgcagatc tgattctagt gatggatcaa
    21601 ggtcagatca ttgaaaaagg aacgcatgag agtttattgg cacaaggtgg attttatgaa
    21661 aaactgtata acagccaatt tgctgaggaa gcagaataag tcatataaga ataaaacagt
    21721 ttaaaactta ttttttgttc tctattcttg ataatggtaa agtaaagtta tcaagaaaag
    21781 tgaggacacg gagatggaac agtttttaaa tgaaaaaatt actgaagcca aaagagaagt
    21841 tgatcgttta acgcacgtct gtcagacgaa gccttttgat tcagctgatt tttttgaaaa
    21901 tgagtttcaa atcgaacgct tggcagcaca aatagaagtg tacgaattaa ttttaaaaaa
    21961 agtaatagaa gaaaatatag atgtacaata aaaacaggta aaacgtcagt taccagatac
    22021 tgtaaataga catctggcct gagcaggttt tacctgtttt ttttcttatg aaataaatat
    22081 atgctatgat acctttattc aataggatta gtttctacaa aaaatgaaag gagaaaaata
    22141 aatatggaac ggtttatagc atcaaatgcc acagtgattg gtgatgtgac attgtcagaa
    22201 gatgtgacga tctggtatca agctgtttta cgcggggaca gtaattggat aaaaatagga
    22261 caaagaacga atatccaaga tggtacaatc atccatgtcg atcatgatgc acctgtagat
    22321 attgcggaga atgtgacggt aggacatcag tgcatgctac atggctgtac aatcgaaaaa
    22381 ggggcgctga tcgggatggg gaccacgata ttgaatcatg cggttattgg tgagaatagt
    22441 ttgattggtg ctggatcatt agtaacagaa ggaaaagtta ttcctcctaa tgtcttggct
    22501 tttggtcgcc cagctaaagt gatccgtcct ttgacaaaag aagaaataca aaaaaacaaa
    22561 gaaaacatcc agcattatgt cgagataggt cggatgcatg cgcaaggaaa attcaaggag
    22621 caaagatagg cagtagcatc cataaaataa aaaaggatgg tcagtttgag ttgaccatcc
    22681 ttttttattt ggtttagata agagagctta aaaaagcttc ctttgaaaca gattaattta
    22741 atgaagaact gttttccatg acttcatcga tcaatccata tgcttttgct tgttcagctg
    22801 tcatatagtt gtcacgatct gtatcttttt caatcacttc aagcggttgt ccagtgcgtt
    22861 cagctaaaat tttgtttaag cgttcgcgtg tttgcaaaat atggcgtgcc gcaatttcga
    22921 tttcagttgc ttgcccttga gcgccgccaa gaggttgatg gatcatgatc tcagcatttg
    22981 gtaaagcaaa tcgtttacct tttgtaccag ctgtcagcaa gaagcttccc attgaagcag
    23041 ccatacccat aacaattgtt tggacatctg ctttgacaaa gttcattgtg tcgtaaatag
    23101 ccatacctgc tgtaactgaa ccaccaggag aattgatata aagatagata tctttttcag
    23161 aatcttgagc gtctaagaaa agcaattggg cgatgatcga attagcaaga tcatctgtta
    23221 cttggccgct taacatgatg atacggtctt tcaataaacg tgagtagata tcgtaagctc
    23281 tttcacctcg tgaagattgt tcaatgactg taggaattaa attcataata gagttcctcc
    23341 ttgatttcgt ttagaaatag tgtaccataa tttagtttct aatacctaaa atgtagtata
    23401 catttttggt caaaaaaggt caaataaaaa acttaggcaa attagtcgca taaacccgct
    23461 ttatggtatg ataagcagag aaacaaaaca aggaggaaca acagattgat agttggtatt
    23521 actggcctta tcggagtgct gacaggtgct gtagttgtct atatcggcat gatactgcaa
    23581 atgcgtcact tggaaaataa agaacagcgt agacgtgaat tggaattgaa agtcaaagaa
    23641 atcgagacgc tgaatcaatt aaataaaaag atcaacgaga tcttgcaaaa acggacaacg
    23701 ctgatggaag aatatgtcag ctttgatgca tttgatgatt gttatatcac gattgacgat
    23761 tttgcgtatc tccaatcatt ttcagcgcaa aatagttttt atctgccaaa ttatatttta
    23821 gacgagctat ttaagaatat ctcccatcga aaagtgattt tatctccaga tgaaacaatg
    23881 aagattggtg gatatgctta taaaggcgga cgcgtgattt tagagaattt tacggatagt
    23941 ctgactgaaa tgatcaatga aaaaaaagct cagttgaaga ctatgacgcg ccagccgatt
    24001 ggtttcttct cgaaagatga tgtttggagc tgatattttg aagcattgcc gttgcacatg
    24061 agagcgtggt aatgctttct ttttttctta atgatacaat agaactgaac ggagtgaaga
    24121 aagatgttac aaatctatgc ccatcgcgga agcagcggca cccacccaga aaatacgctt
    24181 cctgcatttg cagaagctgt acgagtaggt gcagatggta tcgaactaga tgtccatcta
    24241 agcaaagatg gttatttgat cgttatgcac gacgaagaag tggaccggac gactaacggt
    24301 aaagggctga ttcgagaaaa aacattggaa gaactcaaaa aattgaatgc agggggcaaa
    24361 ttcagcaaag aatttccagc agctaaagtt cctgaattga gagaagtgtt aggactgctc
    24421 ctccaaaaaa attaccgagg gatgctaatg attgaaatta aaactgatca gtatgaatat
    24481 cccggtatcg aagaaaaatt agccgaaaca ttaaaaagta gagattggcc ttttcaacat
    24541 gcatattgca gctttcattt ggattctttg gaaaaaatcg cattactgga accggagata
    24601 cagctggact tattgatgag tacttctgat aaaaagatcc aactagcagc agaaacccct
    24661 tatatagaag gagtacatcc aaaaaatact tggttgttcg aacatccagt ccaggcagct
    24721 gattttccaa aagcaattcg tgcatggacg attaacgatg aagaagaaat ggccaaatgt
    24781 caagaactca aaatagatgg aataataact gattttccgg agaaagctct tcggtggaaa
    24841 caatcaacga aaagtaggta aaatatggaa aaagtattag tgatcgtcgg tccgacagca
    24901 gtcggtaaaa cagcgctaag tgtggaactc gcacaactat ttggaggaga gatcatcagt
    24961 ggtgattcat tgcaaatcta taaaaatctg gatatcggta cagcaaaaat caaagaagaa
    25021 gagatgaata atgttccgca tcatttgatt gatgtgatcg aacctactca acgttactca
    25081 gccgttgatt ttcagcattc aggtcgacaa ttgattaccg agattgcgaa ccgagggcac
    25141 ctacctatca tagctggtgg tacgggtctc tatatccaat cccttctata tgattatcag
    25201 ttaggagcga cagaagaagc gaatcctgag attagaaaaa agtatgaaac gttagctgaa
    25261 acaattggtt cccaagcatt atggaatcag ttgaaagaaa aagacccggc agctgctgag
    25321 aaaatccact ggaacaatca acgcaaagtg ataagagctc tggaagtttt agagacgaca
    25381 ggacatagta tcacggcacc taaagaagag ccaaagcaat tgtatgatta cctcatgatt
    25441 ggattagata cgaagcgtag tattctttat gaacgaatca atcaaagagt cgatttgatg
    25501 ctagcagaag gtttagtagc agaagcgcgt aaggtgtatg agcttggaga agtccaggcc
    25561 agtcaaggta ttggatataa agaatttttc ccttattttg aagggaaaga aacattggaa
    25621 gaagcaacag aacaagtcaa actacattcg agaagatacg cgaaacgtca gttgacgtgg
    25681 tttcgtaacc gtttggatgc acagtggttt gatcttataa gtgagccaaa tcaaaaaagt
    25741 cagattgagc aaacaatcaa aaaatggctg gaggcggaat aaaatggaag aaaaagtgat
    25801 catcgtaggg gtagaaaccg aagcaaacca gcgttacttt tcagagtcaa tggaagagct
    25861 agttcagctg acgaataccg catcaggaga agtcgtattc acaattacac aaaagagacc
    25921 acaagtggat cggcaaacga ttatcggtaa agggaaatta caagaattag ttcaacaagc
    25981 ggatgcgcat gaagcggatt tgatcatctt taatcatgag ctaactccga gacagagcca
    26041 actgattacc gatgctgttg gtcttccggt aattgatcgc gtgcagctta tcttagatat
    26101 ttttgcgatg cgtgctcgtt cgaaagaagg gaagctgcag gttgaacttg ctcagttaga
    26161 atacctgctg cctcgtttag tagggcaagg gaaaacatta tctcgtcttg gcggtgggat
    26221 cggaacaaga gggccaggtg aaacaaagtt agaaacagac cgccgacata tacggaataa
    26281 aatttttgct gttaagaaag aattgaaaga agtagaagct catcgagagc gcaatcgcca
    26341 aaaaagaaaa aatagcgaga tttttcagat tggacttatt ggctacacga atgccgggaa
    26401 gtcaactatc ttgaatctat tgacacaagc ggatacttat tcgaaagatc aattgtttgc
    26461 aacgttagat ccattgacta agagatggcg ttttgctgaa ggatttgaga ttactgtaac
    26521 ggataccgtc gggttcatcc aagacctgcc tacgcaactg atcgatgctt tccattctac
    26581 attagaagaa agtcaaaata tggatctttt gctacatgta gtcgatgcta gttctccaga
    26641 tcgtatttta caggagcaga ctgtcttaaa attgatggat gaactgaata tgaaagaaat
    26701 gccgatactg actgtttaca ataaggcaga ccagatcgac ccagcgatgt tcacgcctac
    26761 gctattccca aatgtattga tttctactca gacgaaagaa ggtaagatgg cgttgatcga
    26821 agcaatcaaa cgtcagctga tggaactaat gaccccttat acactttctg tgccaagcaa
    26881 cgaaggacag aaattaagcg agttgagaag acaaacaatg gttttggacg aagcgtacat
    26941 ggaagaaaca aacgaatatg aagttaaagg gtttgcaaaa accaattcaa aatggttaag
    27001 aaaaaaccaa taaaatttta tttttttaa
//
LOCUS       DAZKMB010000025        25543 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000025 DAZKMB010000000
VERSION     DAZKMB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.025"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACG8V3_000981"
     CDS             complement(104..1336)
                     /locus_tag="ACG8V3_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0299536.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACG8V3_000982"
     CDS             1591..2160
                     /locus_tag="ACG8V3_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0299537.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACG8V3_000983"
     CDS             complement(2338..2778)
                     /locus_tag="ACG8V3_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0299538.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACG8V3_000984"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACG8V3_000984"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0299539.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACG8V3_000985"
     CDS             3732..4655
                     /locus_tag="ACG8V3_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0299540.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="ACG8V3_000986"
     CDS             4731..5870
                     /locus_tag="ACG8V3_000986"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0299541.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="ACG8V3_000987"
     CDS             5863..6663
                     /locus_tag="ACG8V3_000987"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0299542.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="ACG8V3_000988"
     CDS             6663..7490
                     /locus_tag="ACG8V3_000988"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0299543.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7495..8202
                     /locus_tag="ACG8V3_000989"
     CDS             7495..8202
                     /locus_tag="ACG8V3_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0299544.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG8V3_000990"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG8V3_000990"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0299545.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG8V3_000991"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG8V3_000991"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0299546.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG8V3_000992"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG8V3_000992"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0299547.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG8V3_000993"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG8V3_000993"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0299548.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="ACG8V3_000994"
     CDS             11787..13820
                     /locus_tag="ACG8V3_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299549.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="ACG8V3_000995"
     CDS             13864..15144
                     /locus_tag="ACG8V3_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0299550.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="ACG8V3_000996"
     CDS             15354..16160
                     /locus_tag="ACG8V3_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0299551.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="ACG8V3_000997"
     CDS             16172..17392
                     /locus_tag="ACG8V3_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299552.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="ACG8V3_000998"
     CDS             17382..18968
                     /locus_tag="ACG8V3_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0299553.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="ACG8V3_000999"
     CDS             19007..21145
                     /locus_tag="ACG8V3_000999"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0299554.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="ACG8V3_001000"
     CDS             complement(21513..22496)
                     /locus_tag="ACG8V3_001000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0299555.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="ACG8V3_001001"
     CDS             22874..24265
                     /locus_tag="ACG8V3_001001"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0299556.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="ACG8V3_001002"
     CDS             24281..25321
                     /locus_tag="ACG8V3_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0299557.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..>25543
                     /locus_tag="ACG8V3_001003"
     CDS             25340..>25543
                     /locus_tag="ACG8V3_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0299558.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKN"
BASE COUNT         8105 a         4317 c         5215 g         7906 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta ttagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aat
//
LOCUS       DAZKMB010000026        25463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000026 DAZKMB010000000
VERSION     DAZKMB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.026"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8V3_001004"
     CDS             133..3660
                     /locus_tag="ACG8V3_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0299559.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8V3_001005"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8V3_001005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0299560.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8V3_001006"
     CDS             7448..7789
                     /locus_tag="ACG8V3_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0299561.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8V3_001007"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8V3_001007"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0299562.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8V3_001008"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8V3_001008"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0299563.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8V3_001009"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8V3_001009"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0299564.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8V3_001010"
     CDS             13000..13737
                     /locus_tag="ACG8V3_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299565.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8V3_001011"
     CDS             13751..14566
                     /locus_tag="ACG8V3_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0299566.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8V3_001012"
     CDS             14579..15226
                     /locus_tag="ACG8V3_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0299567.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8V3_001013"
     CDS             15242..15898
                     /locus_tag="ACG8V3_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0299568.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8V3_001014"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8V3_001014"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0299569.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8V3_001015"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8V3_001015"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0299570.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8V3_001016"
     CDS             18240..18758
                     /locus_tag="ACG8V3_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0299571.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8V3_001017"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8V3_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0299572.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACG8V3_001018"
     CDS             complement(19645..20565)
                     /locus_tag="ACG8V3_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0299573.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACG8V3_001019"
     CDS             20691..21743
                     /locus_tag="ACG8V3_001019"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0299574.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8V3_001020"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8V3_001020"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0299575.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG8V3_001021"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG8V3_001021"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0299576.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ACG8V3_001022"
     CDS             complement(23383..24144)
                     /locus_tag="ACG8V3_001022"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0299577.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ACG8V3_001023"
     CDS             24333..25427
                     /locus_tag="ACG8V3_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0299578.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8443 a         4420 c         5378 g         7222 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa ata
//
LOCUS       DAZKMB010000027        25358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000027 DAZKMB010000000
VERSION     DAZKMB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.027"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACG8V3_001024"
     CDS             complement(132..509)
                     /locus_tag="ACG8V3_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299579.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACG8V3_001025"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACG8V3_001025"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0299580.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACG8V3_001026"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACG8V3_001026"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0299581.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8V3_001027"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8V3_001027"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0299582.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACG8V3_001028"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACG8V3_001028"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0299583.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACG8V3_001029"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACG8V3_001029"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0299584.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACG8V3_001030"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACG8V3_001030"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0299585.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACG8V3_001031"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACG8V3_001031"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0299586.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACG8V3_001032"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACG8V3_001032"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0299587.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACG8V3_001033"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACG8V3_001033"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0299588.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACG8V3_001034"
     CDS             10661..11575
                     /locus_tag="ACG8V3_001034"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0299589.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACG8V3_001035"
     CDS             complement(11708..13273)
                     /locus_tag="ACG8V3_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0299590.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACG8V3_001036"
     CDS             complement(13266..14717)
                     /locus_tag="ACG8V3_001036"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0299591.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACG8V3_001037"
     CDS             complement(14731..15747)
                     /locus_tag="ACG8V3_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0299592.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACG8V3_001038"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACG8V3_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0299593.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG8V3_001039"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG8V3_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0299594.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG8V3_001040"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG8V3_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0299595.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACG8V3_001041"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACG8V3_001041"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0299596.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACG8V3_001042"
     CDS             21340..22764
                     /locus_tag="ACG8V3_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0299597.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACG8V3_001043"
     CDS             complement(22806..23063)
                     /locus_tag="ACG8V3_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299598.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG8V3_001044"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG8V3_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0299599.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACG8V3_001045"
     CDS             24337..25314
                     /locus_tag="ACG8V3_001045"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0299600.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8201 a         4317 c         5696 g         7144 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcgccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAZKMB010000028        24943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000028 DAZKMB010000000
VERSION     DAZKMB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.028"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..497)
                     /locus_tag="ACG8V3_001046"
     CDS             complement(<1..497)
                     /locus_tag="ACG8V3_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0299601.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
     gene            complement(738..3083)
                     /locus_tag="ACG8V3_001047"
     CDS             complement(738..3083)
                     /locus_tag="ACG8V3_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0299602.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            3362..4264
                     /locus_tag="ACG8V3_001048"
     CDS             3362..4264
                     /locus_tag="ACG8V3_001048"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0299603.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            4475..6250
                     /locus_tag="ACG8V3_001049"
     CDS             4475..6250
                     /locus_tag="ACG8V3_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0299604.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(6316..7902)
                     /locus_tag="ACG8V3_001050"
     CDS             complement(6316..7902)
                     /locus_tag="ACG8V3_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0299605.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            8153..8377
                     /locus_tag="ACG8V3_001051"
     CDS             8153..8377
                     /locus_tag="ACG8V3_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0299606.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            8519..10270
                     /locus_tag="ACG8V3_001052"
     CDS             8519..10270
                     /locus_tag="ACG8V3_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0299607.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            10270..12054
                     /locus_tag="ACG8V3_001053"
     CDS             10270..12054
                     /locus_tag="ACG8V3_001053"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299608.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            12389..13241
                     /gene="licT"
                     /locus_tag="ACG8V3_001054"
                     /pseudo
     CDS             12389..13241
                     /gene="licT"
                     /locus_tag="ACG8V3_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            13338..15224
                     /locus_tag="ACG8V3_001055"
     CDS             13338..15224
                     /locus_tag="ACG8V3_001055"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0299609.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(15265..16167)
                     /locus_tag="ACG8V3_001056"
     CDS             complement(15265..16167)
                     /locus_tag="ACG8V3_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0299610.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            16397..17800
                     /locus_tag="ACG8V3_001057"
     CDS             16397..17800
                     /locus_tag="ACG8V3_001057"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0299611.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17800..19233
                     /locus_tag="ACG8V3_001058"
     CDS             17800..19233
                     /locus_tag="ACG8V3_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0299612.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            19378..20304
                     /gene="trxB"
                     /locus_tag="ACG8V3_001059"
     CDS             19378..20304
                     /gene="trxB"
                     /locus_tag="ACG8V3_001059"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0299613.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            20517..22244
                     /locus_tag="ACG8V3_001060"
     CDS             20517..22244
                     /locus_tag="ACG8V3_001060"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0299614.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            22260..22610
                     /locus_tag="ACG8V3_001061"
     CDS             22260..22610
                     /locus_tag="ACG8V3_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0299615.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22704..23564)
                     /locus_tag="ACG8V3_001062"
     CDS             complement(22704..23564)
                     /locus_tag="ACG8V3_001062"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0299616.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23656..24084
                     /locus_tag="ACG8V3_001063"
     CDS             23656..24084
                     /locus_tag="ACG8V3_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0299617.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            24246..24422
                     /gene="rpsU"
                     /locus_tag="ACG8V3_001064"
     CDS             24246..24422
                     /gene="rpsU"
                     /locus_tag="ACG8V3_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0299618.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            24449..24895
                     /locus_tag="ACG8V3_001065"
     CDS             24449..24895
                     /locus_tag="ACG8V3_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0299619.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7878 a         4452 c         5293 g         7320 t
ORIGIN      
        1 taagtagcgt ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca
       61 tttacttctt cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga
      121 ccgtacattt tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa
      181 tggataatag tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt
      241 ttaaactcac cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg
      301 attgatcgaa agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat
      361 ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct
      421 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
      481 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      541 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      601 tctctggttt ttcacatttt tacataaatt tgtcttaata tcgtagatgt aagcttttga
      661 acaccattta ttcggaaaaa gggagctgtg acaagatttt tgtcgcagct ccctttaatt
      721 ctgattaatc tattagatca atcatcttta tcgtctgatt ctttggtctc tggattcgct
      781 ctagcataag ctgaagcggt gttccagtaa tctgtatagt tttcatcagt accgcctacg
      841 ccgaatttaa aggcgctttt cttcggacca ttttttgccc aaagtgactc cactgttttt
      901 cctggcgttt ggatggattt tttattgaca gtaacttttc cagctgtttg tcctgtgaac
      961 ccagatactt ttttcttcat cacatcggat gataagtcaa atttctcatc agtgccgaaa
     1021 atctcagggt ttgcttggta agcttgattg attagattgg caagataatt agccgaacgt
     1081 attcttgctt ggctgtccat tccagtattg tcatcgtgtc ctgcccaact actgatagtg
     1141 atagatggag ttgagacgat cagccatgaa tctcggtatt cattggtaga accagttttt
     1201 ccgacccaat ctgcatttcc tagatttccg tttaagcttg agatgacatc tttgaatggt
     1261 gtagtgattt tttcattgat cacactacgc atcatgtcat tcatgatcga tgccgttgct
     1321 tcggaataaa tacggacaga attactttca tgtttataaa tcacattacc tgcattatct
     1381 gtgatggaat caatgatata gccttcttcg tagactccac catttgctaa ggcctggaat
     1441 ccatttgtct gttgaagcgt cgttacatct actgtcccta aaggagccga ttcaacgccc
     1501 caattatcat ttgctgggta gttcatcttc gccaagtagt gctcgtaagc gtattgtttc
     1561 gatccaccgg aattcaacac atcttgatat aagtgataag ctgggatatt gttggaccat
     1621 tccagtgact cacggacggt ttggaaggtg ttcgaccctt cattggtcgc attgacgatt
     1681 ttctcaccag cattttttcc ttcttgccaa gtcgttgcat aatctgatac acgggattct
     1741 gatccgacaa gtcccatatc gattgcagga ccgtaaacaa gaaccggttt gatggatgaa
     1801 ccagcttgac gttctgtatc aaaggcgtgg ttattttgat tctggctgta attccgacca
     1861 cccacgaagc catagatacg tccggttctg ttatccatca atacatttcc agtctccact
     1921 tggctaccag caccatcatc taacatgtag ccataatttt ctacgccggt ttgtaatgca
     1981 gcatagatat ctttatcgat cgtcgaatgg atcgtatatc ctttgttttg gatcgtttgc
     2041 tgtgcttttt gataataagc ttcactgacc gatgaatctg acatatcagc attatctttt
     2101 tctgccaatt gttgtgcaat aatccttgtt gcttcgttca tcacggtata atacaagaat
     2161 tctctgtcag tctgttccgc gatttgttgt gggagaaagt cttttgtaag atcataggct
     2221 ttcgcttctt cgtattgttc tttcgtaatt tgtccttcac ggtacatact gaataaaaca
     2281 aaatcttttc tggcaagacc ggcagaaaga tcttctttta gcgcgcctgt attcgtgtat
     2341 ggactatacg tgatagggct ttgaggcaaa ccagcgatat aagcagcttg tggcagagtc
     2401 acatcctttg catctacacc aaagattcct ctagcagctt cctggactcc agcaatattt
     2461 tgccccttat tattccgacc aaatggtgag acattcaaat acgtagtaat gatctcattt
     2521 tttgaaaagt gtttttctac ttgtgcagaa agaacgatct cgtttgcttt acgtttgaat
     2581 gttgtttcat cggttaggat ctgttgtttg actaattgct gtgtcaaagt ggatcctcca
     2641 gacgagccga tgccagtcgc ttctgaaact agtgctcgta aaacagcttt agggacataa
     2701 ccttgatgtt tatcgaagta ttcatcttct gtactgataa tcgcggtttt caataaaggc
     2761 gaaatcttat cagatgaaat agtcgtccgc atcagatctg tctggatttt tgatatctct
     2821 gtattatctg cataaacaat ctttgacgtt tccgtaatgt ttcctagttc tgtctgcagt
     2881 tcttttttag tcggcaattg tgtgtcttcg acaagatagg caaagtagcc agcacctatt
     2941 ccgataccta gagctgcgaa caacaaaaga agaacagtaa taaaaacagt caatgattga
     3001 aacactctaa aaataatctt aggaacaaac catttttctt tttttcttct attctgtttt
     3061 tttcgagtat ttttgcgtgg caaaagggtc acctcaacta atggttttat ttcgctaatt
     3121 atagcaaata ataggacaaa aaaacagaca aggctgtttt tgacaggaat ttctttttcg
     3181 aatgagataa aacgtgagga aatgatcttt tttatgaaag agttcttaag cttttcttta
     3241 gggaaagttt cagttactat ggagaacagg gcaaagaaaa acaaaccatt ttctttgtac
     3301 atcatcttat caatatacag gaattgttcg aaacaattgc ctttcaagga ggaatccatc
     3361 catgttatta ggctcacatg tcagcatgag cggaaagaaa atgctgttag gcgcggcaga
     3421 agaagcagcc agttacggat cttcgacctt tatgatctat acaggcgctc ctcaaaatac
     3481 tagaagaaaa gctatcgaag agatgaatat tgatgccgga aaagcattta tggcagaaaa
     3541 cggactaaca aatatcgtcg ttcatgcgcc ttatatcatt aatttgggaa atacgatcaa
     3601 accagaaatg tttccatttg ctgtacaatt tctgagagaa gagattgtcc gtgcggaagc
     3661 tttaggtgct actcaaatca cccttcaccc aggcgctcat gtaggagcag gggcagaagc
     3721 tgggattgct agaattattg aggggttgaa tgaagttttg acaaaagaac agacggcaca
     3781 gatcgctttg gagacgatgg cagggaaagg tacggaaatc ggtcgaacat ttgaggaatt
     3841 agcgcaaatc atcgatggag taaaattgaa tgaaaaatta tccgttactt ttgatacatg
     3901 tcatacgaat gatgccggat ataatataaa agaagatttt tcaggtgtaa tggaagagtt
     3961 tgataaggtc atcggattag atcggcttaa agtactgcat atcaatgatt ctaaaaatcc
     4021 acaaggttct cataaagatc ggcatgccaa tatcggtttt ggaacaattg gatttgacac
     4081 attgaacaaa atcgtccatg atccaatgtt tgacaacatt tcgaaaatat tagaaacccc
     4141 atatgtcggt aaagataaaa agaaagctta cgcaccatat ggaaaagaaa tttccatgtt
     4201 caaagcgcaa gcattcgatc ccgaagtgtt ccctgagtta ttagcagaag aagtcacact
     4261 ttaagttttc tacctgacag caacaaaaaa ggattgggca gtctgccttg tccttttttg
     4321 tttaagaaac aaaggagtgc tttttgcatt cgttttcgcc atgctccttt tgccccctct
     4381 ttttaaattc agacaaacag ggtatactga tagaaaagta aaaaacgaaa agtttcacaa
     4441 acaagtagca aaacgtgatt gaaaggacga aaagatgctg aacattttaa accccttgat
     4501 cgaccaacta aaaaagaaac atacatactt tattcttggc atagccgtat tatttacaat
     4561 tattcggttg tatttatata gtaaagcaat ctatggaaca gatatgtcgg cgacctatga
     4621 tgatcagctg ctgattcact atgctcaaaa tattttggat ggtaaatggc taggggagta
     4681 tacgactaca acactttcca aaggaatctc ctacagctta tttttagtac ttgcacataa
     4741 actgcatctt tcttatgcag ttttgttcgg catgttcagt atttttgcca gtatattggt
     4801 tgcactcgcg tttaaaccga ttgtgaagaa caagtatctt cttttaagta tctattttta
     4861 ttttttgttt tctccgatta gttttacaca tgaatattcc actcgaacat atcgtaatgc
     4921 cctcgtgatt ccggctgttg agatcattat cgcttgttta ttagctattt attaccgcaa
     4981 atacggttct agcagaaaat tgctgccatg gtttttaggg ctttctgtcg cttttccttt
     5041 cttttggttc attcgcgaag attctatctg gcttcttcct tttactgtag tagctttggg
     5101 tatcagtttc gtccagatct tgctagggca agacttttct ttcaaagaag taaagaagtt
     5161 aaaaaaacga ctctttcttc tctcgaaacg ggaatggctg aaactttcat tgtttttact
     5221 tcctatagtt ggcttttcca tcgtctatca gtggatcgaa tataaaaatg agacaacata
     5281 cggtattgca accatcaatg accgttccag tggagaattt ggacgtttga tgaaacaact
     5341 gatccgaata gatgatgaaa cgaacttgaa taaagaaaac agtgagatat gggtctcaca
     5401 tgctgcacta aataaagcat tagccgcatc tcctacattc catactgttt ctacccgtat
     5461 tgacgaatta tatacgactc acgcgtggac tcaaggtgga aaagtaaaag aattacccgg
     5521 agatatcatc ttttgggcat tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa
     5581 tgcaaaagaa accaatgatt tttggaaaaa agtcaaccaa gaattagaag aagcctatca
     5641 caagggaacc atcaaaaaga aaaaagagtt ctatctgatg ggatcaggag atggaaagac
     5701 gatgagtgat ctgccaatcg tattcgattt ccttcaatca ggatacgctt atgcatggcg
     5761 atataaagat ttccatcaag gaggaaatta tagtttcgga acgcaagaac aagtcaaaca
     5821 ggctcaagaa ttattaaacc ttccgctaat ggagaattgg atcgatagta atgaaccgag
     5881 aacacctaat ttccaattaa caaaatcagc aaaagtcgcg aatgtcatca taaaaattta
     5941 ccaagttctg gctattcccg tgctgattgc ttcaatagca gggatattac ttatgatact
     6001 aggaaccgta tttgcaaaag ctgatcgtgc ctattatagt tcctctttga ttgtggtcac
     6061 cggattaata ctgacacaat tggtttttct tttcggtgtc tcatggtttt gttcttatgc
     6121 gccagaaaaa aaggactatt ttttaagtgt ctacactggt gcaggcgtgc cattaacgca
     6181 attcatcgta gtaacaagca tgctagtttt cagtaaatta aatctgaaaa aagctttatc
     6241 ttctaaataa aaagaccctt ggtagctaga tttttaacct aactatcaag ggtctttgtg
     6301 tggaaaaatc agtttttatt cgatttcttc taccgtttcc tgcaaaagct gttcatattt
     6361 tttgtttcct ttatataaat caccaatcgt ccatgccaac aggagaagga caagaacaat
     6421 gattacataa gcgatgtgat cagccaatgc tagttgagcg gaagtggctt gctcgccgaa
     6481 gaaactttcg atctgtcctg gtaatcctcg taagttcaag taagttagac taatcacaga
     6541 gaaccagcca agaagtttta cgatgaatga atttttaaat cggctaccca tctccgcttg
     6601 gctgttggtc atcatcagta aaggaatcat cgaaaatggc aacgcaaaag ccaagaacac
     6661 ctgggattca ttcattaagt tattcaaagc ggcatgttct tcgatcgttc cttttgtact
     6721 tgtaaacaaa acacaaatca atacaggaat cacagaaagc aatcgtgtga ccagtcttct
     6781 tagccaaatc gggatacgca tatggatgaa tccttccatg atgacttgac cggtcaacgt
     6841 accagtaata gtagagttct ggccagaagc aagaagtgct acagcaaata aagtggacaa
     6901 gataccagat tttgctacag caatcaaaat accattgctc atagctgatg gatcagataa
     6961 agcttcatac agaccaaaaa aggaaggatc ttggacagaa ccggttttga agacagcgac
     7021 acccatgatc aatagcagtg cattgactag aaaagccatt gacaactgga tattagaatc
     7081 ccaagtagaa aaacgtacag cttgtgctac atcttcttcg ttttggtgat cgatcttccg
     7141 agtttgtgaa actgctgaat gaagatataa gttatgcggc atgactgttg caccaataat
     7201 ccctaaagct ccagtcagcg gagtttcgcc gccgatgacc ggtgagtctg agaatgtatg
     7261 actgttaggg ataaagccag caaataaact gtgccaatct gggttagaca agccaacttg
     7321 atagacaaaa acgaataaaa tgacgaaaat taagcagaca accagtgctt cgatcttacg
     7381 aaacccgact tttgtcagta gtaagagaac gaaaacatct aatacagtaa taaagacggc
     7441 aatcaccaat ggaatatcaa aaagtaagta gagggcgatt gctgcaccaa tgacttctgc
     7501 gatgtctgtt gccataatgg ctaattcagt caagatccat aaaatgacac ccaatgtctt
     7561 acttgtccgt gctcggattg cttgggccaa atccatttgg gtcacgattc ctaattttgc
     7621 tgccatatac tgtaaaagca tagcaattag actagacatc aaaataactg acatcaataa
     7681 atattggaaa ttttgtccac cagtgatcga agtggaccag ttacctggat ccatataacc
     7741 aactgctact aatgcacctg gtccagagta agcaagaagt gttttaaaaa aactttttcc
     7801 ctttggtaca tccactgttc catttacttc agctaaagat aaaccatttg tatgttcaat
     7861 aagtcgatgc ttatgaaatt gtttgttgga ttgattattc aagaaaaaac cacctttcct
     7921 atttatctac gattacattc tacacctcag aaagaaaaaa ttaaagaaaa agattcggta
     7981 tgcctaattt tttttcgtgg tgtgtcttgc tggttaatga aagaaatttt gttcttctct
     8041 aaaaaaacca ctattttttg catgaaacta aagaatacgc ctttcttttt tcaataaact
     8101 ttagtaaaat aaatcattga gagcaattcg gaaatagaaa ggagagattt atatggtatc
     8161 aacaggtatt gtcgtactta ttgctattat tgctttgcta atcggcgcag taggtggctt
     8221 tttcctagcg cgtaaatata tgaaagacta tttagaaaaa aatcctcccg ttaatgagga
     8281 catgttgcga tcaatgatga tgtcaatggg gcaaaaacca tctgagaaga aaattcgcca
     8341 gatgatgcag caaatgaaga accaagggaa aaaataggtt ctgacccaaa gactttttgc
     8401 gggctgattt tttcagtccg ttttttattt cgtaatttgt tagactattc agactattca
     8461 gcaaaaaaga caagcacaga caatcctatg ctcttgataa agacaaagga ggagtaaaat
     8521 gtcgattttt agaaaattag gttggttttt tcgtcaggaa aagaaacatt atttaattgg
     8581 gattttctcc ctattcgcag tggccttggt acagttagtt cctccaaaag tgattgggat
     8641 catcattgat gagatcgccg ataagaattt atcaataaag atcatttcct ggtggatctt
     8701 attattgatc cttgcagctt tcttacaata tctttttcgt tatatctggc ggacgaatat
     8761 ttggggaagt gcagctcggt tggagagaga acttcggcag cagctattca aacattttac
     8821 agaaatggac aatctctttt accaaaaata tcggactgga gatttgatgg ctcatgcaac
     8881 gaatgatttg aatgcaatcc agaatgtagc aggagctgga atcttgacct ttgcagattc
     8941 agtgattacg ggcggaacaa cgatcattgc gatgattttg tttattgatt ggcgcttaac
     9001 actgattgcg ctgattcctc tgcctttgct agccgttact tcacgagtgc tggggtcaaa
     9061 actccacgat gcttttcgtg attcgcaaga agctttttct agtatcaatg ataagacaca
     9121 agaaagtatc actgggatca aagtcattaa aacatttgga caagaaaaag aagatctcgc
     9181 ggattttgaa gaaaagattg aagatgcaat tattaaaaac aaacgagtca attttttaga
     9241 ctccttattt gatccattca ttaccttgat tatcggtctg tcttatgttg caacaattat
     9301 tttaggtgga aagtatgtct tggaaggcac gatctcaatc gggcagatgg tctcatttat
     9361 tagctatatc ggtatgcttg tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt
     9421 ggaaagagga aatgcttctt atgatcgcgt caatgaactt ttgcatgaaa agacgcatat
     9481 cgtggaacaa ctgggtgcag tgcagacccc ggcaaaaggc gagctgaatg tagctatttc
     9541 ctcttttaac tatccgaaag aagaatcacc agcattagaa caaatcaaat ttgcgatcca
     9601 agaaggagaa acattaggta ttgttgggaa aactggtgcg ggaaaaacga caatcatcaa
     9661 attactgatg cgtgaatacg atgattatca agggaaaatc caatttggcg gacgttcgat
     9721 caaagaatat tcattgaatg ctttattagg atcaatcggt tatgtgcctc aggatcactt
     9781 cctgttttcg atgaccattc gagataatat ccgtttttct aatccaaaac tttcagaaga
     9841 agaagtagag catgcggctt ctttagcatt tatcaacaat gaaatcaagg aattgccaaa
     9901 aggttatgat accttagttg gagaacgtgg agtctcttta tctggcggac agaaacagag
     9961 gatttcgatt gctcgagcat tgatcaaaaa tcctgaattg ctgatcttag atgatgcact
    10021 ttctgcagta gatgccaaaa cagaagaagc tatcttgcat aatttaaaag aaataagaaa
    10081 agagaaaaca accatcattg cagctcaccg tttaagcagc gtgatgcatg caaaagaaat
    10141 cattgttgtc gatcaaggga aaatcattga acgaggaacc caccacgagc tgcttgcatt
    10201 aggtggctgg tatgaacgaa tgtgggaaaa acagcaatta gaagcgaaga tagaaggagg
    10261 cgaggcctga tggatgaaaa ttatcaatcg gaatggacaa aatcgattcc acttaaagaa
    10321 cagaagaaga tcatcaaacg actgatgaag ttcgccaaac cttttagaag aacgttcatc
    10381 gttgctatct tatttgcgtt tgccctgtct gtgatcaatg tcctattgcc aaggatcatc
    10441 caaactttta tggatgatca tctggctaaa cagtcagcta cgacacaagt tattttgttt
    10501 tttgctggtg tgtacttgtt tggagtcata gtcaaaagta tcatttggtt tttccaatgg
    10561 tatttgtatt ccatggcttc attgaagacc tatcagtatg ttcgggtaag attatttgaa
    10621 aagctgcata cactaggtat gcgttatttt gaccagacac cggcaggatc gaccgtttct
    10681 cgagtgacga atgacacaga aacgctattt gaattttggt atgtcttttt gatggtattg
    10741 actgggatct ttgcagtcgt ttcttctttc gttgccatgt ttcaaatcaa tggagaaatt
    10801 gccttgtgga ctttggtgtt cttgccgatt ttaggaatcg tcatctggta ttaccagaaa
    10861 ttcagttctc gtatttaccg aaatatgcgg gaaaaattaa gtcagctgaa cacgaaattg
    10921 aatgaatcga tttccggcat gcgtattatc cagcagtttc gccaagaagc tcgtttagct
    10981 aaagaatttg aacaagtgaa caacgagtat ttagacacgc gctatgcgat gatcaaaacg
    11041 aattcgattc tgttaagtcc aatcatcaat tttctctatg cattggcgat tgcgttggcg
    11101 ttgactttgt ttgggatcga tgcgttgcat tctcccgtag aagttggact gatctatgca
    11161 tttacgacct atgtccagga attttttaat ccaatgtctc aaatgatgga ctttttgagc
    11221 acctttacag atgggatggt tgccggtagt cgtattttga aaatcatgga tacagaagaa
    11281 tatgcaccgc aacaaaatcc tggagcagat caagcgatta cttcaggtaa gatcgagttt
    11341 cgcaatgtaa gcttttctta tgatggagag cacaatgtat tgaacaatat ctcttttgtc
    11401 gctaatccgg gtgagaccgt tgcattagtg gggcacaccg gaagcggaaa aagctcgatc
    11461 atcaacgtat tgatgcggtt ttacgaattt tatgaaggcc aaatcttgat tgatgatcat
    11521 gacattcgag aatatccaat ggaagagtta cgtaagaaaa tgggattggt cttgcaagat
    11581 gccttcttgt tttatggaga tattgcagga aatatccgga tgatgaatcc agctatcaca
    11641 gacgaacaga ttcgccatgc agctgagttt gttcaagcaa acaagttcat cgatgaatta
    11701 ccaaaaggat atcactcaaa agtgatcgaa cacggtgcca gctattctag tggacaacga
    11761 cagctgatca cttttgcgcg aacaatcgtg acagatccta aaatactagt attggatgaa
    11821 gcaacagcaa atatcgatac tgaaaccgaa ggattgatcc aagaaggatt ggcaaaaatg
    11881 cgacaaggac ggacaacgat agcgattgcc catcggctgt ccacgattcg ggatgcagaa
    11941 ttgattttag ttttagacaa aggtcatata gtagagcgtg ggaatcatga aacacttcta
    12001 gcgcaaaatg gtttgtatgc agatatgtac aaactgcaaa gcagtggtgt gtaaaaagag
    12061 gttgtgacag tagcatagta gcattttgcc ctaagcgcta gagtgaaaat aaaacagagc
    12121 ttcgcagatt cttcatctgt gagcgttagt ggcgtagtca cccgatttaa aaaagacgac
    12181 gacagctagt caaaagcggc taataaccac taagaaagcg ttgacaaaag cgtttgcttt
    12241 cgataatata aagaaaacga tacaacttgg atagtgattg cctgatgcaa gctaaacctg
    12301 atgagtctcc catggttcca tggacggctt tttgggtttt tctttttgtt ttggatacag
    12361 ccctatatat catggaaagg agcaaaacat gctgattgag aaagtactga ataacaacgt
    12421 cgtaataacc agggatgaaa gaaatcagga aatgatcgtg atgggaaaag gtttggcatt
    12481 caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc gacaaaaaat ttcagttgac
    12541 aaatgacggg ctttcacaac agttccaaga attggcacaa gcgattcctc ttgagtactt
    12601 agaaatttct tgtgaaatca ttgaatatac aaaagaggct ctacaagtcc agttgaatga
    12661 ttctgtgtat attgcattga cggatcattt gtatacggct atcgaacgtc acaaaatggg
    12721 aatatttgta ccaaatgtat tgctgtatga tatcaaacaa ctatttccta aagagtatgc
    12781 catcgggaaa aaaaacagtg aagaaaatct cggataaata ccacatcaag ttatcagaag
    12841 atgaagccgg atttatcgca ttgcatttag tcaatgcaca aggcattcaa agccaacaag
    12901 acaataccta cctgttcact ataacaatac aggatattct aaatatcgtc cgctattact
    12961 ataagattac atttgatgaa gattcagtcc atttttttcg attcgtcaca cacttacgtt
    13021 tttttctgac acgcgtccta gatggagaaa aatcagaagt gggagaagtt tctgaggaat
    13081 tgtttcaggt tattcaaaaa aaatataagg atacgtttgg ttgcgttgag aaaatagcag
    13141 actttttagc cgagacgttc aactaccaga tgtccacgga cgaaaaaatg tatttaacga
    13201 tccatactgc gcgtttagtg catgagaatc gttgtaaata aataaaaaaa ggaggataaa
    13261 ttaacaagaa cgtatgaaat aagggtagtg acattcagtt ggctaaacct tccactaata
    13321 aattggaggg aatagaaatg ggaaaatacc aagaattagc gagaaaaatc gtagaaaatg
    13381 tcggcgggaa ggaaaatatc aatggtttga cacattgtat cacaaggctt cgtttcaaat
    13441 taaaaaacga agaaaaagca aatgatgaga tcttgaaaaa tatggacggt attgtaacgg
    13501 taatgcgagc gggagggcag tatcaagttg tcatcgggaa tcatgtgcca gtcgtgtttg
    13561 aagaggttat aaaagcagga aaccttactt ttgatgaggc tgtttcaaca gaaaaaatga
    13621 gaccgtttga tatgcttatt gatggaatat cgggctgttt ccaaccattc cttgctatcc
    13681 tagctgctga cgggatgatt cgtggactga cagcattcct tgtcttctta ggcgcgtttg
    13741 accgcggttc tggcacattt gttatgtttg ataatatcgg tgattctgta ttccaattta
    13801 tgcctgttat tatcggttta acagctgccc gtaaatttaa agtaaatgaa ttcgtaggga
    13861 tgttgcttgg cgcagcatta atgaatccta gcttgtcgct ggaagctttg agtggtgcag
    13921 cagaagcacc actaacgacc atattttctg gcactatttt tgaagcacct atttatcaaa
    13981 cagtattcgg cattccctgg attgcgcgta actacgcttc ttctgtcatt cccattatct
    14041 ttattgtgtt gttagctagc caaattcaga aaccaattaa aaagttagta cctgagatga
    14101 tcgcgaattt cttcgttcca ttcttcaccg ttcttattac aatgccttta ggttttttac
    14161 tcgttggacc agtattcaca ttcgcaactg atattttaat ggctggattt gaaacacttt
    14221 tagcactttc gccagttatt tatggagcaa tcgtaggctt cttctggcaa attctggtga
    14281 tgtttggtct ccattgggca atcgtaccaa tgggattgat gcaattttct gtgaatggtt
    14341 ggcaaaatat tatgacacct gttgcagtag tgtcttttgg acaaactgca gcacttaccg
    14401 cactctactt taagcttaga aatccaaaag acaaagcaat tgcaatacca gccattgttt
    14461 ctggtattgt ggggattaca gagccagcta tttatgggtt tacattgcca cgtaaaaaaa
    14521 tatttatttt tacctgtgtc ggtggtgcgt tagggggcgc gtatagtggg ttaatgaatt
    14581 taacttcttg gaatcaaggt ggactaggca tctttacaat tcccaactat attagaccag
    14641 atggtgattt aacagatgtg attaatgtat tgattgggat cgccattgcg atggtggtat
    14701 cctttgcgct gacttttttc ttttggaagg aggaagcaga tgaaacagat gatagacaga
    14761 aaagatcagg taaagaaatt gtaaaaacac cgatacaagg acaaatcgct ccactgaatg
    14821 cagcaaagga tgcagctttt gcacaaggaa cactaggtcg aggaatcttg atctaccctg
    14881 aaaaaggaga agtacgtgcc ccttttgacg gaacagtcat gtccctgttt ccgacgaaac
    14941 atgcaatcgg gatggtttct gaagcaggtc tggaattgct gattcatgtc ggattggata
    15001 ctgttcagtt ggaagggaaa tattttgaaa gcttggtgca gcaaggagaa agagtgaaac
    15061 aaggcgatct gttgcttcgc ttcgacaaaa agaaaatcga agaggaagga tacagtttag
    15121 aaacacctgt gattgtcatc aattacatgg attatttgga tattttggaa aatcaagggg
    15181 aaacggtcgg gccagacgat gcacttatca ccgcactgac ctgaacggtt aggtgaaaga
    15241 atggactagg cttaactatt ctttttagtc cacttcttta ttttcaaaat tgaaaggctc
    15301 tttcatgatg gctgagctga tagggcgttt atcaaatttt tgtcctacag aaattagatg
    15361 gactaaattc tgttgataat tttctgcaaa aacacaacta tataacacgt ctagaagata
    15421 acaaatcgat gtattgatcg taaaattacc aattttcgaa tacagtcgtt cgcgtgtcgt
    15481 aagtcgcaaa gcggctgtac tcattgaaga tagcgttgaa tcaccgatac tggtcaaagc
    15541 taaaattggc acatgatttt gtttcaatat ttctgcggta cgaaggatca cattgttttc
    15601 acccgtataa gaaatcagaa tcccacacat atcacttgtg caattgtacg cttcatagaa
    15661 cgattcacca aaagtggtga caatcgtgac atccattttg atgcgcttca ttttcagcat
    15721 gaaatcttga gaaatcaaag tattcgagtt actggcgaaa atcacgattt tttttgcttg
    15781 aagcattagt tgtttggctt tttgcagttg gtcatgatga atgagagaaa gtgtatcttg
    15841 aatcgtcatc tgttctaaag aagccatttt atttgcgatc gtcataatac cgtctgtggt
    15901 ggaaaaagga agattcgcat ctacgtcttg aaaattccct tcaagatagg ctgtttcagc
    15961 taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc attttttttg ctacacggat
    16021 gagtgtggac gggtggacat aacatcgctc agcaatctct tggatggtca tttcgccaag
    16081 tgctggagaa tcgcttaaga gaaactgaat cacttcattt tctgaaggag aaaaggagat
    16141 ttctttgatt ttttcacgta ataacatgta gataccttct tcttgttttt ttacgtaaat
    16201 cataactttt ttgaaaatga tttgtaaacg gatttatgtt atatagctta ataaagacat
    16261 tttttgacaa gatatttaca aaaatccaga agaaaacact aacttttgta aatgttactg
    16321 gaaagaaacg aatttctatt gtttgttttg tttacaaaaa agataataaa agtgtaaaaa
    16381 gtaaaggagt gagaacatgg caaaaggaat caaaatcgct acgattggtg gaggcagcag
    16441 ttacaccccc gagttgatgg aaggatttat caaaagatat gaggaattac cgatacgaga
    16501 aatctggctc gtagatgtag aagaaggaaa agaaaagctt gagatcgtca gtcagatgtc
    16561 tcagcgtatg tgggatgctt ctccgtatga agtcaaaatc tatgcaactc tggatcggga
    16621 agaagcattg aaagatgcgg attttgttac gactcagttt cgtgtcggtc aattagatgc
    16681 acgaatcaaa gacgagcgga tccctagtta ttatgggatg attgggcaag aaacaaatgg
    16741 tgctggcggg atattcaaag cttttcgtac cgttccagtg gttttggcaa tcgttgaaga
    16801 catgaagcgt ttatgtccaa atgcttggct gattaatttc gcgaacccaa gcgggatggt
    16861 gacagaagca gttatcagat atggcaaatg ggataaagtg atcggattat gcaatgtgcc
    16921 agtaatggcg atgatgacag aaccaacaat gcttggcaag aaaaaagaag agctgatcta
    16981 caagtttgcc ggtctaaatc acttccattg gcacaaagta gcagataata aaggaaatga
    17041 ccgcacggca gaattgattg atcgtttata cgatgaagac aatggattgc cgaaaaatat
    17101 ctttgacatc ccgtttttca aagaacaatt aaaacaaatg aacatgatcc cttgtggcta
    17161 ccaccgctat tactatcgtg aagaagaaat gctggaacat gctttagaag aatatcaaac
    17221 gattggtaca agagcgcagc aagtgaaaga aacagaagca gagctgtttg aattatacaa
    17281 agatccagaa ttggatcata aacctgaaca attgcaaaaa cgaggtggtg cttattattc
    17341 agatgctgcc tgtgaaacga ttgcttcgat ctatggaaat aaagaaacac agatcgtggt
    17401 atctacaaag aatgatggag ctgttcctga tctgccggca gattgtgtag tagaagtcac
    17461 tgcctatgtt ggcgcacaag gcgcacgaaa tgtagcgttt ggttcccttc ctgctgctga
    17521 aagaggctgg ttgcaagtaa tgaagaatat ggaattgctg acgatcgaag cagccgtgac
    17581 tggtgattat ggaacagctt tgcaagcgtt tacgatcaat ccgcttatac gatcaggaaa
    17641 gacagcaaaa cggatcatgg atgagttgtt tattgcacat cagcagtatt taccgcaatt
    17701 caaggaaaca atcagccggt taaaagcaga aggaatcgaa gtccaagatg aactggcaag
    17761 agaattatca agcaatacac atgtaatgga ggaagtatga tgggattcag aaaagatttt
    17821 ttatggggcg gagctacagc agccaatcaa tgcgagggcg gttatgatga aggcggacgt
    17881 gggttagcga acgtagatgt tgtgccaatc ggagaagacc gttttcctgt cattactgga
    17941 aaaaagaaaa tgtttgaatt tgatgaaaat catttttatc cagcaaaaat tgcgatcgat
    18001 atgtatcatc attataagga agatattgct ttatttgcag aaatggggtt caagacttat
    18061 cggatgtcca ttgcttggag tcgtattttt ccaatgggcg atgaaaaaga gccaaatgaa
    18121 tctgggttga aattttatga ggatgtcttt aaagaatgtc aaaagtacgg gatcgagccg
    18181 cttgtgacga ttacacattt tgattgtccg atgcatttga tcaaagaata tggtgcgtgg
    18241 cgttccagag aactcgttgg tttttatgaa aatctgtgcc gagtcatctt caatcgctat
    18301 aaaggacttg tcaaatattg gttgacattc aatgagatca atatgatcct tcatgcacca
    18361 ttcatgggcg cagggctgta ttttgaagaa ggagaaaatg aagaacaaat caaatatcaa
    18421 gcggcacatc atgagttggt tgcaagtgcc atcgcaacga agatcgctca tgaagtagat
    18481 ccggagaaca aagtaggctg tatgctggca gcaggagcat attatccata cacttgcaat
    18541 cctgaagatg tttgggcttc tagaaagtca gatcgagaaa attatttctt cattgatgtc
    18601 cagtcacgag gagaatatcc cgcttatgca ttgaaagaaa tggaacgaaa tggcattcag
    18661 atcaaaatgg aacctgggga tgccgcattg ctgaaagaac atactgttga ttttatctcc
    18721 ttttcttatt attcttctcg agtttcgact actgacaacg aactcttgga acagactgca
    18781 ggaaatatct ttgcttctgt caaaaatcca tatctggaag caagtgaatg gggttggcaa
    18841 attgatccat taggcttgag aataacgatg aatgatattt atgatcgtta tcaaaaacca
    18901 ctatttattg tagaaaacgg cttaggtgca gtcgatacgc ccgatgagaa tggttatgtg
    18961 gaagatgatt atcgaatcga ctatctggca gcacatatcc aggcaatgaa agatgcagta
    19021 gaattagatg gagtcgatct attaggctat acgacttggg gatgtattga tttagtatca
    19081 gctggtacag gtgagatgaa gaaaaggtac ggattcattt atgtcgatcg tgacaacgaa
    19141 ggaaatggca cattagcccg ttcgaagaag aaatcattca actggtataa aaaagtaatt
    19201 gattcaaatg gagaaaatct ttcgaacgat tgatcaatga tttataaaag tctattgttt
    19261 ttcagcggat ttttcgagta taataaagct attcgagaaa cgcaagcaga acaatagact
    19321 ttttttcgca ttggctgtaa agtatgctta cttgattaag taaaggagaa tctacatatg
    19381 tatgatgtaa tcgttattgg cgcagggcct gctgggatga ctgctgcttt atatgcttca
    19441 cgttcaaact tatccgtatt gatgatcgaa caaggcgctc ctggcggcca aatgaacaat
    19501 acggcagaaa tcgaaaacta tcctggtttc gattcaatca tgggtcctga tctagcttat
    19561 aagatgtatg aaggtgtttc aagatttggg acagaaaatg cttatggaat cgtgcaagat
    19621 atcaaggatc acggcagcca caaagaagtt atttgtgaag ataaaactta cgaaggaaaa
    19681 acagtcatta tcgcaacagg ctgtatccac cgtaaattag gcgtacctgg cgaggaagaa
    19741 tttgctggac gtggcgtttc ttactgtgca gtttgtgatg gtgcattttt ccgtaacaaa
    19801 cgtctagtag tgatcggagg aggagactca gcagtagaag aagccatcta tttgacacaa
    19861 tttgcttctg aagtagtcat cgtccaccgt agagatgaat tacgtgcaca aaagatcatc
    19921 caagaccgtg catttgcgaa tgaaaaaatt tcctttatgt gggatacagt cgttgacgaa
    19981 atcgttggaa atgaaatggt agtgacaggt gtgaaagcac gtaatgtcaa aactggcaat
    20041 gagtttgatt tggcagcaga cggagtattc atctatgtag gattggatcc attaactgca
    20101 ccatttagag aagctgggtt gacgaacgaa gccggctgga ttccaacaga caacgaaatg
    20161 aagacgaata taccaggcgt ttttgccatc ggagatgttc gtgaaaaaga tttgcgacaa
    20221 atcactactg ctgttggtga tggtgggatt gctggacaac aagtgtataa atacttggaa
    20281 tctttagaag aaacaactgc ataagtttaa aaagagtgaa agaacggttt caattgttct
    20341 tttactcttt tttttgcgtg aaatctactg aatccgattt atgcgaatac ttgtaaccgt
    20401 gcttgataaa agaatctttt cttgatttaa gcgttttttg gtcctcatac tttcatatct
    20461 aataagtgaa tgctatactt attaagaacc atatttcaga aagaggtgtt attttcatgt
    20521 cttgggaaca agtgtatgaa caatgggtac atgaagaaaa tttagaagaa aacttaaaaa
    20581 aacaattaaa tgatctgggt gaagatcctg aaaaacttga agatgctttt tatgcaccgt
    20641 tggaattcgg tactgcaggc atgcggggaa tcttaggacc tgggatcaat cgaatgaaca
    20701 tctatacggt aagacaagca acagaaggac ttgcacgttt catggataca caaggtgaag
    20761 aaacaaaacg ccgaggtgtc gctatcgctt atgactcacg ccatcaatca ccagaatttg
    20821 ccatggaagc agccaaaaca ttagcacatc acaatatccc ttcttatgta ttcgaaagtt
    20881 tacgtccaac accagagctt tcttttgcag tccgtcattt gaagacgttt gcgggaatca
    20941 tggtaactgc atcccacaat ccagcagctt acaacggata taaagtctac ggagaagatg
    21001 ggggacaaat gcctcctgca gacgcagatg cattgacgaa atatgtccgt gaagtcagca
    21061 atccgttaaa agttgaagta ttatctgacg acgaagctga acatagcgga ttgatcacga
    21121 tcatcggaga agaagtggat gccgcttatc tagaagaaat caaagcagta acgattgatc
    21181 atgaattggt ggaaacaatg ggtaaagatt tgaagcttgt gtacacacca cttcacggaa
    21241 caggaaaaat gttgggcgaa cgtgcgttga aacaagctgg attcgaacaa ttcgtactag
    21301 ttccagaaca agctgtcgca gacccggatt tcactacagt caaatcaccg aatccagaag
    21361 aacactctgc tttcgagtat gcgatgcgac ttggcgagaa agaaggcgct gacttactga
    21421 ttgcaacgga tcctgatgca gaccgtttag gcgcagccgt tcgtttgcca aatggctcct
    21481 atgaagtatt gacaggaaac caaatcggag ccctgtttgc tcaatatatt ttggaagcac
    21541 accaatcgcg aggcacttta cctgaaaatg cagcggtatt gaaatcaatc gtttctagcg
    21601 aattgcctac agcaattgcg gaacactatg gggctaccat gttcaatgta ttgacaggat
    21661 tcaaattcat tgctgaaaaa attcaacaat ttgaagaaga tcattcccat acttttatgt
    21721 tcggattcga agaaagttac ggttatttag ttaaaccttt cgttcgcgac aaagacgcga
    21781 ttcaagcatt gctcttatta gcagaagtag cagcttacta taaaaaacaa ggcaaaacat
    21841 tgtatgacgg cttgcaaacg atttttgaaa catatggcta ctacgcagaa aaaacgattt
    21901 ctgtgacaat gagcggacaa gaaggctctg caaaaatctc tgctttgatg gaaaaattcc
    21961 gtgaagaagc accgacagaa tttgccggag caaaagtcat ccaaacagaa gacttcaaag
    22021 agttgacgag aacagaccgt gaaggaaacg tagaaaaact aacgacaccg ccatctgacg
    22081 tattgaagta tacgctggaa gaaaatggct ggatcgctat ccgtccttca ggaactgagc
    22141 cgaaaatcaa attttatatc ggtgtaaaag gaaactcttc agaagacgca gctgaaaaag
    22201 tagccgctta tgaagcgatt atcaaaaaaa tgacagaaga ataaaaaaac ttgagactga
    22261 tggaaaaagt ggtaaacttt agaagtcagt cttttttcaa gttggaggtg aatggattgc
    22321 cagacacgca aaaaacaaaa gtagagaaag tcagccaact attcaaaatg ttgagtgacc
    22381 ccactcgtct caaaattctg ctgtatctga aagacgggga acaaaatgtg acagcaatca
    22441 cccaagcagt agaaatggaa caatctgctg tatcgcatca actgcgttta ttaagagaaa
    22501 accatgtcgt caagtcccat cgtgagggaa aagcgatttt gtatagtctt gatgattctc
    22561 acgtactgga tattttgaac cagacgctca aacatgtgga acatcaataa cgataggtgc
    22621 aaatagagac aaactggact gtatcaacga atcttcttcg ctgacacagt ccagtttttt
    22681 taagaaaaag acaagaaaaa ctgttagtct gtttcttcgg tggtttcagt tccataataa
    22741 ctatgatctt ctaggtctaa agcattcaaa ataagtgaag ctgtttcttc gatagataaa
    22801 gaagcgacat tgattactac gcagcccaat ttttcataaa gatcattcgc aaaatcgagt
    22861 tcagctcgta ttttttcgat atcggaataa gcagtatcag gatttaatcc gtaagagcgc
    22921 atccgttctt tacggatatt gtttaaaata tctgggttat tcgtcagtcc cacgattttt
    22981 ttaggatcga tctcccaaag ctgtttggga atatgtgctt gtgggatcaa aggtagattg
    23041 gcaaccttta aatttttgtt agctaaaaac aagctcaaag gcgttttgga agttctggat
    23101 acgcctagca aaacgacatc tgcttctaga aacccacgag gatctttccc gtcatcgtat
    23161 tttacagcaa attccattgc tttgatccgt ttgaaatagt tctcattcaa taaatgaagg
    23221 gcgccaggtt gccgaatggg cttgatacca gttaagcgct cgatttctga tatcggcgga
    23281 gtcatcacat caaaactaaa taattgatgt tcttcacaaa aagaacgcgt catctcgatc
    23341 aattcttgat tgatcaatgt gtgtaggaca agggcttttt cttttagtgc atcttgcagt
    23401 gcctgcatca atgttttctc atctgtcaca aatgttctgc ggaataaatt gaactcaacg
    23461 gtaggatatt gggccatagt ggcttgcgct aattttgtcg cagtttctcc agcagagtca
    23521 gagatgacaa aaaaagtaat gatttctgta gaatcggaag acatggataa aacctcactt
    23581 gcattttttt tatgtttatt ataccatact ctagaatgtg tacttcgtaa tcattttgat
    23641 ttggaggaat aagtcatgaa aaaacagaca ttgattgaac aatcactaga aacattgaaa
    23701 aataacggtt ttaaatatac taaaaaaaga gaagcattgt taacttattt agtgaagcgc
    23761 aatcgctacg tatccgctaa agaagttttt gactatatga atcaggaatt caaaggagta
    23821 agttacgata cgatttatcg aaatctccat gattttgcag aaatcgattt actggaagaa
    23881 accgatttaa atggagagat gaagtttcgt tttcattgct gtcaagggga aatcggccat
    23941 caccatcact ttatctgtac ggtgtgcgga acaacgaaag aaatccatat gtgtccaatg
    24001 aattatttcg aagaacagct cggtggatgt acgatcgaag ggcatagatt cgagattttc
    24061 ggaagatgtg aaaattgtca ataaggattt aaataaaata acatttgata attttaaaaa
    24121 aaagcaagat ttccttgacg gtcaaaacag cattagatat aatatttaca ggacagtctt
    24181 gttttttgag atatatttca ataacaatta cggttacttt ttaagctcgg agggagggaa
    24241 ttaacatgtc aaaaacagtc gttcgcaaaa acgaatcact tgacgatgct cttcgtcgct
    24301 tcaaacgttc cgtgtcaaaa gcgggtactt tacaagaatc tcgtaaacgt gaattctacg
    24361 aaaaaccaag tgtaagacgc aagaaaaaat ctgaagcagc tagaaaacgc aaaaaattct
    24421 agttctgtga tgaattggag ttggtagtgt gtcactacta agtagattaa acgatgacat
    24481 gaaaacagcg atgaaagcga aagataagga atctttacaa gtcatccgaa tgatcaagtc
    24541 atctatacaa aacgagcaga ttaaagaagg tcacgacttg acagaagaag aagaattaac
    24601 ggtgttgtct cgcgaaatga aacagcgacg tgattctttg catgagtttg aagaagcagg
    24661 acgtgacgat ttagctgaaa aagtaaaaag tgaaattgtg atagtcgaaa aatacatgcc
    24721 ggaacaactt tccgacgaag aaattcgaca acttgttcaa gaagcaatca cacaaactgg
    24781 tgcttcatca atgaaagaat ttggcaaagt catgggtgcg atcatgccta aagtcaaagg
    24841 aaaagcagat ggaaaccaag taaatgccat tgtgaaagaa ctattacaat cttaatttta
    24901 tcatttaaag gctcttcgtc aatgagtgtt ggtgaagaaa ttc
//
LOCUS       DAZKMB010000029        24893 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000029 DAZKMB010000000
VERSION     DAZKMB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.029"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..1920
                     /locus_tag="ACG8V3_001066"
     CDS             226..1920
                     /locus_tag="ACG8V3_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0299620.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            2608..4485
                     /gene="ltrA"
                     /locus_tag="ACG8V3_001067"
     CDS             2608..4485
                     /gene="ltrA"
                     /locus_tag="ACG8V3_001067"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0299621.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            4616..4993
                     /locus_tag="ACG8V3_001068"
                     /pseudo
     CDS             4616..4993
                     /locus_tag="ACG8V3_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            4993..6198
                     /locus_tag="ACG8V3_001069"
     CDS             4993..6198
                     /locus_tag="ACG8V3_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0299622.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            6214..6858
                     /locus_tag="ACG8V3_001070"
     CDS             6214..6858
                     /locus_tag="ACG8V3_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299623.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            6869..7675
                     /locus_tag="ACG8V3_001071"
     CDS             6869..7675
                     /locus_tag="ACG8V3_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299624.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            7697..7927
                     /locus_tag="ACG8V3_001072"
     CDS             7697..7927
                     /locus_tag="ACG8V3_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299625.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            8473..8748
                     /locus_tag="ACG8V3_001073"
     CDS             8473..8748
                     /locus_tag="ACG8V3_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299626.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            8790..9212
                     /locus_tag="ACG8V3_001074"
     CDS             8790..9212
                     /locus_tag="ACG8V3_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299627.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            9231..10823
                     /gene="mobP2"
                     /locus_tag="ACG8V3_001075"
     CDS             9231..10823
                     /gene="mobP2"
                     /locus_tag="ACG8V3_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0299628.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            10835..11170
                     /locus_tag="ACG8V3_001076"
     CDS             10835..11170
                     /locus_tag="ACG8V3_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299629.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            11317..11511
                     /locus_tag="ACG8V3_001077"
     CDS             11317..11511
                     /locus_tag="ACG8V3_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299630.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            11543..13915
                     /locus_tag="ACG8V3_001078"
     CDS             11543..13915
                     /locus_tag="ACG8V3_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0299631.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            13976..15436
                     /locus_tag="ACG8V3_001079"
     CDS             13976..15436
                     /locus_tag="ACG8V3_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0299632.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            15447..17651
                     /gene="topB"
                     /locus_tag="ACG8V3_001080"
     CDS             15447..17651
                     /gene="topB"
                     /locus_tag="ACG8V3_001080"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0299633.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            17667..17816
                     /locus_tag="ACG8V3_001081"
     CDS             17667..17816
                     /locus_tag="ACG8V3_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299634.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            17890..18483
                     /locus_tag="ACG8V3_001082"
     CDS             17890..18483
                     /locus_tag="ACG8V3_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0299635.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            18499..19398
                     /locus_tag="ACG8V3_001083"
     CDS             18499..19398
                     /locus_tag="ACG8V3_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0299636.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            19401..19886
                     /gene="ssb"
                     /locus_tag="ACG8V3_001084"
     CDS             19401..19886
                     /gene="ssb"
                     /locus_tag="ACG8V3_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0299637.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            20252..20512
                     /locus_tag="ACG8V3_001085"
     CDS             20252..20512
                     /locus_tag="ACG8V3_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299638.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            20953..21159
                     /locus_tag="ACG8V3_001086"
     CDS             20953..21159
                     /locus_tag="ACG8V3_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0299639.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            21159..21410
                     /locus_tag="ACG8V3_001087"
     CDS             21159..21410
                     /locus_tag="ACG8V3_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299640.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            21425..21862
                     /locus_tag="ACG8V3_001088"
     CDS             21425..21862
                     /locus_tag="ACG8V3_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299641.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            21855..22562
                     /locus_tag="ACG8V3_001089"
     CDS             21855..22562
                     /locus_tag="ACG8V3_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299642.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            22727..22927
                     /locus_tag="ACG8V3_001090"
     CDS             22727..22927
                     /locus_tag="ACG8V3_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299643.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            22943..23122
                     /locus_tag="ACG8V3_001091"
     CDS             22943..23122
                     /locus_tag="ACG8V3_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299644.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            23463..23726
                     /locus_tag="ACG8V3_001092"
     CDS             23463..23726
                     /locus_tag="ACG8V3_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0299645.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            23720..24070
                     /locus_tag="ACG8V3_001093"
     CDS             23720..24070
                     /locus_tag="ACG8V3_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0299646.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(24094..24708)
                     /locus_tag="ACG8V3_001094"
     CDS             complement(24094..24708)
                     /locus_tag="ACG8V3_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0299647.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         9283 a         4006 c         5153 g         6451 t
ORIGIN      
        1 ccactctaca aaaaagaatt gatcaagatt ctgtatcgaa tgaacaatat gaatgatgag
       61 taggaatgga gaagagagaa tgaaatatcc gttgatatgg ttagaaaaag atgttccgaa
      121 ggaagagacc actagaaaat ttcctcacac tttaagcctg ggtaagttgg gaaaagggca
      181 atcttttaac caagtaaaga atcaaacaag aagaggtgac tgtacatgtt agaagcaatt
      241 aaaacagctt tttcaccaca aaatgatcaa aaagtacttg atccattaaa gcaactagga
      301 aatcgggaaa tagaaattgt aaaatccaaa gggtatgatt tagatttttt aaaacaagtg
      361 atgccacaag gtgggttaaa ttttgatgaa gaatatgtga cgtatggtaa tggttattca
      421 cgatgtgtga cagtttatca ctacacaaaa aatcctacgc ttttttggtt ggccaatctc
      481 atgaacaatc ctcatacagt ggccacactc gatgttagca cagatgaaaa gcaaaaagtg
      541 attgatcaaa tcaatcgtgc cttagatgaa ttaaatgatc gatcagataa tgaacgaaaa
      601 gcgactgacc gaaataaaag cgcggaagaa tacctggaac tagcggaata tgctagaaaa
      661 ttgacttcta atggggaaat ttctaagcaa gtcaaagtga gagtacatgt ttacggaaac
      721 acgattcaag aagtagatga acgagcgcag gaactgatcg aaaatctcaa agggatggat
      781 tatcgtgcgg tgacttatct gcataaaacc aaacgagatt ggcaatcgat gtttaccatg
      841 ttcgatgaac aagctgaatg gttcggaaat atcaaagggc aatcactacc atcgttaaac
      901 attggcggag gtttcccgtt ccaccaccaa acgctaaaag atccaggagg gacacaatat
      961 ggggaaacca tgacaggcgg cccatttgtc tggaatgcga cgtataaaaa tagtgtacgg
     1021 ttgtctacta ataccttgct tctaggaatg atgggagctg gtaaatcgac cgttcttaaa
     1081 atgatcgcgg aagcccattt agctgcagga gactttattt ggggctttga aaagggaaaa
     1141 gactttattc ctttcttaaa ggaatataac ggtattatgg tgcgtttaga cggatctgac
     1201 ggaatgatca atcctttaga gatatttgcc acacggacgt atgatgaagc atccagttta
     1261 tatgacgatg gatcagtcaa agatttgaag atcaatgaag cagcgtccta tcaaacacac
     1321 ttagacaaag tggtctatca ggtacagtta gtgagtcctc agctaaaagg aacgatgaaa
     1381 gcagaattta agacctactt aaatcgtttt tatgaagagt atggaacagt accacgcggc
     1441 tttacttcct caaattcgcg ttcaaatact gatacgcaag tcacagggaa agatccagaa
     1501 gcttatccga cgttcaaaga atttttagac tttttaggac aactggaatt accaggtgct
     1561 tcccaagaga aaaagaatcg aaaagaagaa atggaatcca tcgtagaatc cttatgtgag
     1621 acgtatggca tgatttttga tggtcatagt acgattcgac accttgacca acaacagttg
     1681 gtcttttttg acattgactc tattacaggc ttagatcgag gggtacaaca atgtcaaatg
     1741 tacatggcta tgacgttgat ttggaatcag gcattgattc aaggaagaag acaacgacgt
     1801 ttaattaaag aaaaacaaat ggaagaacaa gacttcaaac gcttcttggc gatgattgat
     1861 gagtgccata atggtgcgac ctgttctgca gtgaaaagct gtgggttgca agtagtctaa
     1921 aaactattgt aataactctt ataacgagaa gtcaaatgag tgggtaacgc cacaaagggc
     1981 ttccactgat actccgacat gcaacaagta gtttatgcag accaacgttg tgtgaagctc
     2041 ggtgaagtcg gctgaaggat accgtaaggt aaaagaaata gtgaatacca cgaaagttct
     2101 ctagaggtaa agggtgtatc cgataagtcg ggtgtccaaa aaaacttaat atggtgagta
     2161 tgtacaaacg tactgacgaa ctagcgaatg tactgtccga aaggcatagc tcctagaaat
     2221 gggagagaca gtgagcaaaa aatcactcat tgtgctaact tgtggttgag taaaagtcgt
     2281 tgttatgaaa gaccatgctg cgttacaggc gtaggagaag ttagggggca aaagctagca
     2341 tctaagttaa ggcatgtgga tagatataag ggaacagggg aagccatcaa catggagagt
     2401 gcatactcga tgaagtggta gtaagaaata tgtgaatgac aaataactta ctttagtgat
     2461 ggtgaaagta gagtcacgac tgatgaaggt tctgtaatgg aactggagga acaggctcga
     2521 ttcgtctgta aaagtcatga aacccgttca accatgggat tctcgtatga ctaaaaaggt
     2581 cacgagccaa ttggagcgtg ataaaccatg acaaaagaaa caagtccaaa agataggaag
     2641 ttgcggcatg cagaatatta tggcatgacg gaaatcttcg ataagatata ctccaaaagt
     2701 ttgaagggag ataatttcaa gaatctaatg cagattatca tttctgataa caacattgtc
     2761 ctagcatatc gtaatatcaa gagaaatcac ggtagtgtga caagaggaga agatggagta
     2821 acgattaaag atattgaaaa tctcaaccgt aatgaattcg tagaaattgt taggaagcga
     2881 ttcgcacatt ataaccctcg gaaagtaagg cgtgtagaaa ttcctaaata taatggtaaa
     2941 ttaagaccgt tgggtattcc atcaatatgg gatagaatcg cgcagcaatg catcttacaa
     3001 gtattagaac caatatgtga agcaaaattc aataatcaca gctatggttt ccgcccaaat
     3061 agatcagcag aacacgcaat agcagattgt atggcccgaa tgaatcaaag tcacatggaa
     3121 tatgtggtag atgtagatat caaaggtttc ttcgacgaag tcaatcatac taaactcatg
     3181 agacaaatct ggtctatggg tatacgagac aaacagcttc tcttaatcat taggaaaatg
     3241 ttaagagcac ccgtagtact tccaaatggg aagttgatat acccaacaaa aggaacacca
     3301 caaggcggaa tacttagccc attacttgcg aacatcaatc tcaacgaatt tgactggtgg
     3361 atagcaaatc agtgggagga aaaagatgcc aaagagctaa aacatcattt taggaaaaat
     3421 ggagcttggg ataaaggtaa tatctatcac aagctcagaa agagtacaac tctaaaagaa
     3481 atatacctag tgagatacgc agatgatttt aaaattttct gtagaaatta taatgaagcg
     3541 aaaaaagttt tctatgcaac taaaatgtgg ttacaggagc gtctaaagtt accaatatct
     3601 gaagaaaagt caaaaatcac aaatctttca aaagaagaaa gtgaattttt aggctttacg
     3661 atgaaattag tgaccaaagg taacaaaaag gttgttaata cgcatatagg tacaaaagca
     3721 caaagcatga ttaaagtgaa actaaaaaaa caaatcaaac gaattcaaaa aagtcctaat
     3781 agcaaagata cctttatcta tattggagtc tataacagta tggttattgg gattcataat
     3841 tacttcaaaa ttgcgactca ttgtagtgta gcaatgcgaa aaattgcaag agaactcaga
     3901 ccaagttttg aaatacgact acgagaacaa ggaatctcaa aagaaggaca atacggaaag
     3961 tacgaaggat acaagcctta cctacggtca ggacaaatac gatatataag agggtttcca
     4021 atattaccag tagggtatat tcaacacaga catccgatgt gtaaaaaaag aaatattaat
     4081 aaatacaccg aagaaggtag ggaagccatt cataagaagc aacaaaccgt agaagaatgg
     4141 aagatatcgt ggttgtgtag caatccagtg cttagtaaga gagcaacgat tgaatttgcg
     4201 gacaatcgaa tttcaaaatt catcgctcaa aagggaagat gtgcagtaac aggtgaggag
     4261 cttatcttat ctgaaatgca ttgtcatcat attatccctt atcatgaaag caaaagtgat
     4321 agctatgaaa acctagtgat tgttactgag gaggttcatc gggtaataca cgctacacaa
     4381 agtgaaacga ttgaggaact tctaaaatat ctaaaattaa atccaaaaca aaaagaaaaa
     4441 ctcaatgagt tgagattaaa agttggtaac gaagaaatat cttaataaat atttgtagga
     4501 cgggaaaaca gaatgtcttt tatagacgag ggaacgccgt atgcggtgaa agtcgcacgt
     4561 acggtgtgga ctgggggaaa agacggagat aatatcaaac tcttacctat cagtatcgat
     4621 taatccagat tttattgaaa cggtaaatta tgtcacaaac tttcaacgag aaatgcgtaa
     4681 agtagaagca atgactgttt tagctacaca atccccacaa gaaatggtgc cagaaaatat
     4741 gtcttctgat aacttctcga aactgaagaa agtctttgaa ttttcttcta cgaagatctt
     4801 tatgcgtatg gatgaatcta ttttacgtca tatagaagga ttagtgggta aatcaatgac
     4861 gaactcagaa ttagaatcca ttcctcaaca aaatcaagga gacgctgtga tcaattttgg
     4921 ctcaaaagaa accattcgtg ttcattttag tccgaacaaa cgtcaattaa aattgtttga
     4981 tggaggaaaa taatggaaaa ccaaaatgaa agtttaatca agcagtatgt taagcgtcgt
     5041 gcaaagcggc gcttattttt gtggttgttt ggtacttctg caggtttgat tactattttg
     5101 atcactgtgt tcgtgaccct atttctaata cttgcggcgg gctcgattga taattcggat
     5161 tcagattcaa gcagtggtgg cgaagctttt acaggagaat attcggaagg cttaccgatt
     5221 tataaagaaa tcaaaggaag aggccctttt tctgatgaga ttgcgcaata tgccgtaggt
     5281 gcagcagtga aatataaatt attaccaagt gtgatccttt cgcaatacgg ttacgaatca
     5341 gcgtttggta catcggcgtc cgcaagaaat gatctcaact attttgggat tacttggttt
     5401 gatggttgtc tgtttcctaa aggaactgca cgaggaattg gtggcattga aggtggctgg
     5461 tacatgaagt ttcccaactc aaaagctgca ttttcctatt acggctttat ggtagccaca
     5521 caatccaatt ttaatgcttg tgtaggaaat aaaagccctg gcgcaagctt attgatctta
     5581 ggacgaggcg gttatgccgc tgcaggaatt acggaagata gtccgtatta tacaggttgt
     5641 atgtcaatta tcacttctaa taaattaacc gaatatgatg aattcgccat caaacattgg
     5701 ggtgaaggtg gcaataacaa cggcacgatt actggtgaat ggacgaatcc attccctggc
     5761 agcagtttag ataaaagttc cttcagcggt ggtcaattat ttggaacgaa tccaggcggt
     5821 gaattccgtc cgaacggctt ccatgatgga ttagattttg gttcagttga tcatccagga
     5881 agtgagattc atgcggttca tggcggaaaa gtcgtgtacg ttggcaatcc tggtatttca
     5941 ggtttgggtg cgtgtgtgat cgtgattaat tacgatggct tgaatatggt gtatcaagaa
     6001 tttgcaaata gtacaggaaa ttctcgagtg aaagtaggcg atcaagtgaa agttggtcaa
     6061 gtaattggga tacgagatac agcacacttg catttaggat ttactcgaat ggattggcgt
     6121 caagcacaag gacatgcatt cattgatgat ggtacatgga tcgatccgtt accatttttg
     6181 aatagcagta aaaaatagat caggagaaaa aatatggaaa ataaaacgtt aaaaatcgtc
     6241 ttaacagctg caattagcgt tagtttaatc ggcaatattg cgttgttttg gcagctgaaa
     6301 gaggaacaag cagcaaatac ctataaaatt gaagaagtgg ctaaaaacgc gtctgagaag
     6361 ctcaaaaaaa attcctctga ggcacaggcg aagatcgaca agttaaatca aaccatcaat
     6421 gagaaaaacc aagaaatcga tcagctgaag caacaagatg gcacaaaagc aaaagaggaa
     6481 gtaactgaag cacaaagaaa gacggcagaa gagttcggtc aattggccat tgataaatca
     6541 ttaagcagaa atgaattaac ggaaaaacta aaagatgtcg caacccaaga agtgatcgac
     6601 aaattaagtc ctacagacga tgatcaccaa catgatgatt acggaagtta ctcctttaca
     6661 ttaggtgacg tgcaaactta cgctcaaaca agcagtgtaa aagaggaaca aaattttgtc
     6721 gtatttgttg attatacaat cggcaatccg caattcaaag atgtcaaacc acaaaaaata
     6781 aaaggtggat taactgtcac tgaaaaacaa gtagatggtc aatggaaagt catagacttt
     6841 agttatttca ctcgttagga ggaagaaaat gagtaagaaa aaaggagaaa gacaacgttt
     6901 caaaaaacga aaaagttatc ttagatggct gggaatcttt atgattctcg gctttttgat
     6961 tcttgctagt ccgatttata cgaatcagaa ggtagatata ccagatgagg taagcacgaa
     7021 cgccttttta aacacgaaag agctgacaat ggtttcaaaa gaaaaaggag cgaaccagca
     7081 attagttttt gaatttgcga tcgatgatga agatcaaagc ttgttaaaag aactggctaa
     7141 tcttaattat cgcgtagaag taaagacagc taaaggggat tatcaagcaa tacgagccaa
     7201 agtgattaag gtgtctgatg attattttgc agtgaagcta acgcatgtac cagaagaata
     7261 tatcgccatg cgtttaacca ttattcctga aaaaattgat ccaaaagtgg atatgcaaga
     7321 accacaagat ctgatttttt atattcacga agataaggtc aaagatcacg tgaaagacga
     7381 agattatgaa caacatgcga tcaattacaa agtgaaagga tacaagaaag aacaaaaagc
     7441 agctcaaaaa caaatggaat ctttccaagc aaccattgat ttgaatgaag aattagtgaa
     7501 gaagctaaaa gatcaattgc cttatcaagt cgcagatgat caggaaaata ctgaaaacaa
     7561 gattaatagc taccagcaag aaattgaaac atcgaaaacg caactgaaag atcaagaaga
     7621 gatcattcaa aaacttcaag aaaaaattga tcgtatgaca aaagaaaaga tttagaagga
     7681 caggtggagt gaaaaaatga agcgaacaaa cttggctgac aaagatactt tcgctgagca
     7741 gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat gtcgagaagt
     7801 gaatttatct aacacaacaa tatctgagtt gaagaatggt cgaaagaatt tagatacctt
     7861 aaaattcaga tcatttgaag ctctatatgt agctgctaaa aaatatttta cagctgataa
     7921 caaataaaaa acaggagtat tttatgtgaa aaaagtatat aaaaaatgct tatgaatcag
     7981 ctatatcacc ttaaccattt gttaatgaga tgggagaaaa cattaaccac gattctaacg
     8041 caaacatcaa gcacataaaa catttaacac tcctcctttc tggctgaaga tttcaaccaa
     8101 acaggaagac agcgtatcaa ttatagcata cactttttct taaattcatt tttcatatga
     8161 caaaatattt ccttctaatc gacaaagtta gaaggatttt ttattgtcta aatacacaaa
     8221 taaccttaat ttttaaagag cataaggaaa cgtgatatca ctttctcacg aaaggtgtta
     8281 ggtaggctgt gcctaccacg tttctaagaa agataaatga tatcaaaatt ttggatttgt
     8341 gtgtagattt gtgggtagga tgaaagcgaa tgtgggcagt aaaataagcg tttgtgtgta
     8401 gcaaaatcaa aaaatgtgta gggatatgtg tagctacaca tatccctaca cataataaat
     8461 ttggaggaaa atatggactt attaattaga aatattgaag aaaagtatat taataaaatt
     8521 gacaaacgtt gtgaagaact tacggtcaag actggcaaaa aatggagtcg aaatcaatac
     8581 ctaaaagttt tgatcgaaaa tgattttgat cacgctcttc tgaattataa aaaagaccaa
     8641 tttgatcggt tgttagaaaa atttgtggat attcaaactt ataatacgaa aactttagaa
     8701 gaatatattg caacaaataa tcaattaatt ggtttgttaa ttgaataata aaaatagtat
     8761 tgtgaattta taaaaggagg aaatcaattt tggcaaaagc aacaagaaaa catgtatatc
     8821 tttacgatat gcatatcgaa gtacttgatg aaataatttc tactcatgaa gatatatcta
     8881 attatagtga agcagtcaga catttgtgta tgatgtatga tccaaaaatc gaagaaaaaa
     8941 taaatccaat tcaaattaaa ttgaatgcaa tgggaaaaga aatttctatt ttaaccgaaa
     9001 tggtttcaga gtttgcggat cttcatttga aagatacagg aatactaatt ggtacaaatt
     9061 caactgttta tcaagaagca gaagaacgtg tggaaggaaa aattaaatcc aatcaaacca
     9121 aaaaatattc ttctaagtta aagaaaccag tgaatgaatc tgttcatacc agagtagatc
     9181 catttgatcc aacgaagatt ctaaaaagat aaaagtagaa ggagaacgtg atgaataaat
     9241 cgcctgcagt tattcttact tgtcaattta ccttgcccaa taaaaaatct ttttcaacat
     9301 acattgatta tatgacaaga gaaaaagcac tggaagaaat ggaacgacgt acgcccgaag
     9361 aagaacagga actgcaacga atcaagaatg ctttagagga tttttatatt cctgaaggag
     9421 aaactttttc ggaaagtaag acggataaag aattcagtga aacgtcgaat gaagctcaat
     9481 cgttgatgga acatggaatc aattttgaca agttagagga acaagatttt accaaatatc
     9541 tttcttatat gacccgtcac tatgcgttag aacaaaagaa agaactgacg caaagggaac
     9601 aaaaagaata ccaacggttg aaccaagcaa tgaaaaaata tcaaacagag ccacataaaa
     9661 aagcttcagg aaatcttcct ggagtttttt ctatggcttc agacgaagtg aaaggaagtg
     9721 atctgaagga gatcaaacaa atcttccaaa gaggacaaca aaaaggctca atcatttatc
     9781 aagatgttat ctctcacgac aattcgtatc ttgaaaaaat gggcttgtac gatcccaaaa
     9841 cagatacctt agatgaagaa ggtttgaaag cagctggaag aaaaatgatg gagacattat
     9901 tcgagaaaga acaattgaat gaaacaggtt actggatggc aacgattcac cgtaacacta
     9961 agcatatcca tattcatttt gcagttgttg aaaaagaaaa tacgcgtaaa ccgtatactg
    10021 agattgaaaa tggtgtgagt tatatcgtac cgaaggggaa gagaagacaa gcaacattag
    10081 acgcaatgaa aaccaattat gtacatgagt tagaacgttt ctcttatgaa aaggatcgaa
    10141 tgatcggtat ggagaaacga aatttattga cacgaaaaag tgatttgcgt aacaccttaa
    10201 caaaagtagt aaaagaacca gcacgttacg atcgacacgc catgaactta ttaaatgaag
    10261 tatataaaac cctacctgaa aaacgttcgg aatggaatta tggagatgaa aatcgaacca
    10321 agctttcacc atttacaagg ggaaaacttg atgagttaac caaatatgtt ttaacaaaag
    10381 aacctggata tcaagaatat atacagattt caaaagcatt gaaagaagaa gccaaaagcc
    10441 tttatgggga aagcaaaaga gaaacaaaga atgcagaaaa aaacgccctc tttgatttaa
    10501 aaaagcgcac aggaaatgcg attttaacag aactgaagag acaggatagt caaataaaac
    10561 cattaataga aagtgtcaat cagatgcaat ctatttacaa tgaacgatcc tttcgaacac
    10621 tcaaacagtt gccaacaaat gaatacaagt atagttatca agaattcgta aagcgtagga
    10681 aacaattcca acaaagagtt gtcacgcagc gaagcttgcg tcaattaaat cgaacaatta
    10741 atgcacatga agaaaaatac cacgcaagta aagcatatga agagcaacag cgaagaatcc
    10801 aaatggaaca agaaagacaa taggaggaag agaaatgaaa caatatttag atcaatggaa
    10861 agtcatagaa ggatcactaa aagaagaacg aatcgaacaa cttcctgatt gtttagaaaa
    10921 agagcatttg tttcagataa gcgaaatgtt gagaaacgaa cagtttgatc caaatcaatt
    10981 tttagttgtt gagtacccag ctactggtgt gtattgttgt aatcatgtga agggtgaaaa
    11041 atattttatt attcaagaat acgaaggtaa gttagcgcct tattatacta cttgggaaat
    11101 aaatgaagag ggaatcaata atttcccatg tgaaagcatt gaagagtcta tttctctcac
    11161 ggaatgttaa ataaatagag acgtaaaaga gtcctgaacg tgattgtcca ggactctttt
    11221 tcaatgaatt gttttccgcc aattcactga gtggtgcgat tcaattttat tatatcaaaa
    11281 gaaagtgaga ttccaaagtg aagggaggaa gaagaaatga ctcagacaac gattggtgtt
    11341 gtggttgaaa aaagcaggga taatctcgtt tttgtgacag aaattcaaac aggtagagct
    11401 tttgtagtca cagacaaagc tgcaaaagcg tatcaaaatg gcaatatttt aacgttaaat
    11461 ctgactacga agatgttcgt tgatgcagta gaaaattatc catttgttta gacaaacgat
    11521 attttacaag gaggaaacgg acatggcaga agaaaaagaa ccactagata atagtcggtt
    11581 gggaaagtcc aaaagaaaat tagtacggtt gcaaaacgaa ttaaataatc aatttcaaaa
    11641 gctatttgat caccaaaaat tgacaaatgg gcaaccaatg aatgacaaac gaaatggtcg
    11701 ttcatggttt aaccagcaag aaaaaataga aaataaaata caaagtttga gagaagagat
    11761 caagcaacaa gaaaaacagg tagaaaaact tgaaagacaa gaagagctaa aggaaatggg
    11821 ctataacaag tacggcggac tggatatgac gatcgataat attccaagaa tcaaagaaga
    11881 aatcgaacgc tttgaaaaag gtgaatcgac tttttcagcc gccacgatcc gaaaatatca
    11941 acggaagttg gaaacattag aacagctgaa agaaaaatca gaaaaaggaa aagaaaatat
    12001 cctccctgaa gtacaagcga tcattgacag cggacgagtg acgcaatgga aaaaaaatcc
    12061 aaccatttat tttttgaaag gctatcgaaa agttgcttta gaactgaatg aaaacggggg
    12121 atttgaggaa tcaaaaaaat accaagccac gtcggaagag gaaaaagcag ctgttcgtga
    12181 attacttgaa gaggctgcac caactgattc tctgaaggag aaagaaaaag atcatttcta
    12241 tcgagtagaa ttcaacgaaa atgatccaga aaaaggattg aaaagttatg gtggggagat
    12301 cgtaacatta gaattgatcg atcaacttaa aacgttggat aaccaatacg ctgcagaagc
    12361 aggttattat aaattctatt ttgaagaagt gaagaacgga gaagtagtag atcagatccg
    12421 aatggatcta ggggatggcg tcaacagtaa tcaccccatc tatttagagt tagcaaaatt
    12481 gtgcgaacaa gtacctgaga aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa
    12541 agaacaaatg cagtcgaaag aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc
    12601 agaagagatc aaacaagaag ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa
    12661 gcatacccat tcttctgaag acgtcaaaga acttttgaac tttatgtctc gttttccaga
    12721 acgatccttt agaaatcaat tattgattga ggaacaattt ccaggtgcaa cggcttgttt
    12781 aggtcgtgcc gcgttgaaaa aagaaggaat ctatatcaaa aaaggggaac aagggaacaa
    12841 gatctttgtc agaaaaacgg tcaaaggatt ttatgagaaa ggacgtggct ttgttcgaga
    12901 aacagaagct acaccagaag ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa
    12961 aaaaccttac tacacgattg aaaaagtgtt tgatatcaca caaacacagt taaaacctga
    13021 agactatccg aaaatctttc cgaaccgtgt ctttgatttt caattggata aaaaaggaca
    13081 gtcagaactt caagctggga ttcaagcggt agccaacaat attggaatac aaattcgaga
    13141 tatgacagaa agtgaagtct atcaaagaga attaggacaa gcacgtgggg cgtatgttcg
    13201 taacgaattt actggcgaag aagaaatcgt catgaatact cgaaatactc ctacgcagca
    13261 tttagctaca agtattcatg aattagccca tgcaagaatg cacaaatttt cagaattaga
    13321 taccgcaact aaagaattgc aggcagaaat gacttcttat attgtgtgca aacattttgg
    13381 aatggacact tcggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac
    13441 gttggatgat aaggaagcgg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc
    13501 aaatgaattc attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga
    13561 gatccaacca gcagaagtga agcaggagca taaaaatact gtagaaacgg cagagaataa
    13621 gaatattgtg cccgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga
    13681 agatacgggt cgaacgttac atttgaagga tgcggcgttt attgaagatc cgcagcataa
    13741 tcaagtgatt ttaaaaggaa aaagttatgg aaaagagatt caaacggcgt taccttcaga
    13801 agaatttgcg ctaggaaatc cattccgatt ttcacttgaa acagtgtcag aagtggccaa
    13861 acgaaaaaat gaaatctatt atgaacaaca attgtcacaa aaagaacgcg tataaaacaa
    13921 gtaaaaaaga ctatctgaaa aacggatagt cttttttctt ttgggaggtt tgaaaatgac
    13981 cgaaaaaaaa atgagtctta ttgatcgatg taaacagatc gatatagtgg attttgctcg
    14041 aaacaatgga atggctgtag tcaataaagg acgagactat cgcttagaag accatgattc
    14101 atttgttttt gatcgaagaa aacaacgttt ttattggaac agtcaaaata ttagtggcga
    14161 cattattgaa ttagctaaac tttttttcat agacaaagag atccaagact ctaagcaaca
    14221 atttaaagca gccactgatt ttattctaaa gaatgaagac aaaacagaac gagtggaaaa
    14281 tcttcatttt gaaacagaaa agtacaaaga tcaccccgtt gattatcaac cactcacaga
    14341 aaaaggaagg aattatctga aggaagaacg aaaactacca gagtggctga ttgattatgc
    14401 agaaaaagaa gggttgattg ccgaactaaa gccaaaatat gaacggcaaa atttcttagt
    14461 tcgagacgat cgtttagatc atgcggtcgc ctttttatgg aaagatccac agacaaaaga
    14521 gacagtaggg gcgagttatc aaggaacgtt cattgattat gaacgttttg gtgagagagg
    14581 cacatataag catatagata aaaattcgac agcgaatcat ggctttaacc taaagatcgg
    14641 tgaccctaag cagttaaaat tttttgagag tagtattgat ttacttagct atgctgcact
    14701 gaatcgtgat cagttaaacg atacgtggtt agtctctatg gaagggttaa aacaccatgt
    14761 gatcagtcat tattttggtg aggcagtatc tgaattgaga aagaaacaag cctttcctca
    14821 atccatcgag atttgtgtgg ataatgaccg tgcaggacat attttctacg aaaaagaaca
    14881 attaatgggc gcagtcgatc catttacgaa tcagaaagta cgttgtgaac gaggaatcgc
    14941 taatgattgg caagtgccaa aagaatacaa aatcatttat gaagaagtcg caaaagaaga
    15001 aaaggtcaca ccagaagcta ttatggccat acacaagaca gaaaacaatc tgcaattaac
    15061 gaaccaactg gtttccgctc acaaagtaaa tgcgtttttt ggccagcagc tttctgttaa
    15121 cgattcaata gaagccatta atttgaaaga tatttgtaga gaagtcgcaa aagaattaaa
    15181 agtttgtgaa cgcgtggatg gtacgtacga ttttgatcgt ttttatcaag aaaaagggga
    15241 tattaatgcg cagattcttt tctcttataa agcagaacaa tactacaaag gctataaaaa
    15301 tcatgaacat gaatttatac ctgaagtaaa aaaggactgg aacgatcaat tgaagcatga
    15361 gatccagcaa caagaaatca gaaagcaaaa acgtgccatg ttgtttcaac aaggcagaca
    15421 acaagaaagg gagtgaaatc gtcatgatga aaaccgttat tttagcagaa aagccgtcac
    15481 aagcgaaagc ttatgccgat agcttttcta aagctacccg aaaagatggg tattttgaga
    15541 tccaagatcg attgtttaca ggagaaactg tgatcacgta tggttttggt catttagtcg
    15601 agttagattc gcccgatatg tacgatgaaa aatggaaaca atgggcatta gaacatttgc
    15661 ctattttccc caatcaatac cactatcatg ttcctaaaga taagaaaaag cagtttagtg
    15721 tcgtcaaacg acagctgcag tcagctgata cgattgtgat cgcgactgat agcgatcgtg
    15781 aaggggagct gattgcctgg tcaatcattc aacaagcagg cgccaatcaa ggaaaaacct
    15841 ttaaaagact ttggatcaac tccttagaaa aagaagccat ttatcaggga ttccaacagt
    15901 tacgagacgc agaagagacc tatccaaaat ttgaagaagt acaagcacgt cagatcgccg
    15961 attggttaat tgggatgaac ggtagcccac tgtatagttt actcttgcag caaaaaggca
    16021 taccaggaag tttttcttta ggtagagtcc aaaccccaac actctatatg atttatcaat
    16081 tgcaggaaaa gattagaaac ttccaaaaag aaccatattt tgaaggaaaa gcccaagtaa
    16141 tcgctcaaaa tggcgcattt gatgcgaagc ttgatccaaa tgaaacccaa gcgacacaag
    16201 aagcatttga agcctattta aaagaaaaag gtgttcaatt aggaaaacag ccaggtacga
    16261 tccttcaagt cgaaacagag aaaaaaagtg cggctagccc acgtcttttt tctttatcga
    16321 gccttcagtc taaaatgaat caactcatga aagctagtgc caaagatact ttggaagcca
    16381 tgcaagggtt gtatgaagga aaatatctaa gctatccgcg aacggatacc ccttatatta
    16441 cggaagggga atatgcgtat ctgttggatc acttagacga atacaagcat tttttaaaag
    16501 ccgaagcgat tccgactcct attcatacac cgaatagtcg gtatgtgaat aataagaaag
    16561 tccaggaaca ttacgcgatc attcctacaa aaacggttat gacagcagcg gcttttgaac
    16621 aactaagtcc actgcagcag gctatttatg aacaagtgtt aaaaacaacg gtggccatgt
    16681 ttgcggaaaa gtatacctat gaagaaacaa cgatcttgac gcaagttcag cagcttcagt
    16741 taaaggcaat cgaaaaggtg ccgctagatc taggttggaa gaagttattt ggaaaagaga
    16801 gcgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg
    16861 tgacggtgga tctacaagtt cttgaaaaag aaaccaagcc accacagcct tataccgaag
    16921 gtacgttgat cacggcgatg aaaacagctg ggaaaaccgt tgatagtgaa gaagcgcaat
    16981 ccatattgaa agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa
    17041 ccttgaaaca aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag
    17101 ggattttgct ttgccaagcc gtggaaaaag agccgctgct tacgagtgcc gaaatgaccg
    17161 ccaaatggga aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc
    17221 tagccaatat ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat
    17281 ccactgattt tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg
    17341 cgcgtcattt aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg
    17401 tggcagcatg tacgtctgaa gcctgtgatt tcaaactatg gacaacgatt gccaaaaaga
    17461 aacttacagc gacacagctg aaagagatca ttcaaaatag ccgaacaagc caaccagtga
    17521 aagggttaaa aggtcagaag ggttcgtttg aagcaacgat tgttttaaaa gaagacttca
    17581 ctacaggctt tgaattttct gaaaagaaaa aaacgaactt caagaaaaga acacgacgca
    17641 ctacaaaata atggagggtt aatcttatgc gtaatacaac acacaaacaa caagaaatcg
    17701 aaaatcaaaa agccaaaatt cgttcagaac aggaaaaaat gcgtgccagc aaacgaagac
    17761 tgcagcaatt gaagaaccag cagcggattc aaaaagaaat cgaattagat cgataaattc
    17821 ctttcttgtt aatataaaac taacatgtta tagttgtgtt agttttatat taactttagg
    17881 ggaagagtaa tgaaagacaa aatccaatgt atttttgata aacataatgg caacctatcc
    17941 ttgaaggatg ctagaagtga aggattatcg cctatgacgt tagatcgatt agccgaaaag
    18001 gataaaatag aaaaggtagc accaggtttc tataccttaa aggatgcgtt cgtggatgaa
    18061 ttgtatctcg ctcagagtat ctattccaga gggatttttt ctcatgagac tgcgctcgat
    18121 ctttttcaag taggaacata tatccctaaa aagatcaata tgatgtttcc taaaggatac
    18181 aatgtatcaa aggagaaatt agatcagtat gccattcaac cgcattacac taataaagaa
    18241 aattttgacc tgggagtcac agaaacagaa agcttttacg gcaataaaat tttagtctat
    18301 gatttagagc gaacgttatg tgacatgtgg aatccaagat acaaagcaag cgtagaggta
    18361 aaacaagaag cattaaaaga atatatgaca agtaccagta gaaatacaaa taagctaaga
    18421 agatacatgg atattttaaa atcaccaaaa gaaatgactt tgtatatgtt gccactttat
    18481 tgagaggagt aaagatgaat gacaccgaca caatttaaag agaaaacaag aaatttcgca
    18541 gcagagaaac atttaaaccc acaaattgtc caaagatatt atatgatgga aaaattatta
    18601 gaatgtattg ttgaatcgcc ttatcatgat gattttgtat taaaaggggg atttctgatt
    18661 ggatcaaaat acgggttaga aaaccgttca accgttgata ttgatacgac ttatcgaaat
    18721 tcgaaactaa caaaagaaaa gttagaaact gttttttctg aattaacagc taatcctaca
    18781 aaagatggaa ttcgttttga attaaaggga ttaacagaga cgagggaagc cgattattat
    18841 ccaggttttc aagcaaagtt tattgcttat cttgaaaatg cacgtgtacc ttttaaatta
    18901 gatattactt caggtgattc tattattcca gcagcattga tgtatgaaca caaattaatg
    18961 tttgaagata aaacgattca tattccagct tatcctacgg aacaaattct agctgaaaaa
    19021 ttacatgcca cgtttagttt tggaaaagat aattcgcgca tgaaagattt ttatgacctg
    19081 tatatgattc cgaaattaga acgtatcgat gagaaaaatt tgtataagag tgttcaagga
    19141 acattcaagg aaagaaataa tcaatcggtg tttaagcatt tgtatgatca ggaaatgcct
    19201 caattggtga aagatgaggg cttgagagaa aagtggaaaa gataccaaca agaaaactat
    19261 tttgccaaag acatttcttt cgaatctaca ctaacttccg ttgattccct gatgcaatca
    19321 attgtagagc aagagaaggc agaacgaaaa caaaatttta agagatcaca acaaatgaat
    19381 caagaaatgg agagataaag atgatcaata atgtaacttt agtagggcga ttaactaaag
    19441 atccagattt acgttatacc gcaagtggca cagcagtggc gactttttcc ctagccgtta
    19501 atcgtaattt cacgaatcaa aacggcaatc gagaagctga ttttatcaat tgcgtgattt
    19561 ggcgcaaacc agcagaaacg atggcgacat tagctaaaaa aggcagcttg atcggcgttg
    19621 tcggacgaat tcaaacacgg acttatgaca accagcaagg acaacgtgtg tatgtgacag
    19681 aagttgtggc cgataacttc caactattag aaagtaaagc agcgacagaa agccgtatgc
    19741 acgctgatca atcgtccagc agtccaagcc ctaccacctt tgaacaacgg gatacagcga
    19801 cgcctaacaa caatggattg aacgcttcac agaatccatt tggtggacaa tcaatcgata
    19861 ttagcgatga tgatctaccg ttctaagtag cattttctat tggttgcatt tgctacggtt
    19921 tatgttataa tttagacaac aaaaagaatt ttcaatctat aaccaaaaac cactagctgg
    19981 cgggctagtg gctttctttt acgtttctat tataagaatt aacgggtagg cttactgtct
    20041 ttcttggcaa atttacaatc aagccatttg cagatatagt gcgcggctac ttctgccatg
    20101 attgcaaaaa caaaatcaaa aagaattttc aaacctataa cctcctcccc ttgccagatt
    20161 aggccggggt agcagacagc aagactatta tagcataatt gaaatatcca aatgattgga
    20221 tttttgctat aattttagcg aggtgattgt gatgaacaaa tatgttggtt tgcttgataa
    20281 aatccgagta ataaaaacgc aaccgttatt ggttcgcttt actttacaaa cgattcatga
    20341 atcgattaat tgtgtagttg cagatattga aattatcgat aaattgctaa ttatggatga
    20401 tggaaaatac aatattgccg tcacaggtca cttcaataaa cgaaatcagc tagttattgc
    20461 tagcatgtat gttagaaatc ccgatcattt tacgcgatcg atgggcatat aaataaaaga
    20521 agagttgtag tcctaaatga ggattgcagc tctttttgtt ttacatagaa acaggaaaag
    20581 acctttttag gaagacacaa gtgccttctc aattgaaaaa cctttctctc actccaaccg
    20641 ccaccgccgt tcgagaagaa aggaagtgaa cgctgcacgt tcactaagat atggtgtctt
    20701 gcaaagcaag acaaaataga caaaacttcc tctcaccaaa aggttcgttc ctgttcgtta
    20761 tctctttatt gatttgccag accggcaaca agttgccttt tatcaccaaa aagcgacaag
    20821 gacaagggta aatggcacgc tgcgcgccct tgtccttgtc gcttttcgtt gatttattct
    20881 ggccaacaaa tcaataaaga aagaactaac gaaaggaagt aaaacctttc ctaagaggag
    20941 gcagaatcaa acatgcgaaa gaaatttgat tattggggtg ttcccttcag cgaattattc
    21001 ccaaactatc atgcaccaca tactgttgaa tgcgattgtg gagaaagagc gaaatgtatc
    21061 aaaagctatc gtttgtatca atgtccaaca tgcgggaaaa aatatacctt gtcttatggc
    21121 gattacattt tgattgatga gaaaggaaag aaacgctgat gaaaacacaa cacaaattat
    21181 tgactgtaaa agaagtcgtt caaattttgg tggacaaaga agtcatttct gaaaaagtag
    21241 cagaagcttt acttattccg cggtactgga cgatagaaac ccctttagta gaggatgcac
    21301 cgactatgct atttgaagac ctagtgaaga ttgcaggata tgaactgcat tatcaagaaa
    21361 acatggaaac ttatgaaatc gaatattggt taaccaaatt agagaactaa aaaggagaaa
    21421 aaatatggct caaaattact atgacgaatt tgtcaaatta cctttggata aaatggcaca
    21481 aaaaatggag gatatgacgt tcctatacca tgaaacacgt gtacccaaaa aacattacaa
    21541 ggaaaaattg tcagtcgcag tggaagaaat gattgaatca ggtgtcgaaa tgaatctcat
    21601 tgcgacgtac tatcgaacac tggaagaact gaaaaaacaa aatggaaaat ggtttttcca
    21661 agccttacta tgcttggaag taggtgtcaa accaagcact atcaaacctt ccgaatatca
    21721 agccttagaa ctgacttatg caaaattcgc acagattaag aaagctaaag caatggctag
    21781 cgactggcta gatacttttg aaagtatcga aaaaaatggt gcattttata cgattgaatc
    21841 ggaggagaaa gataatgaat aaggtggttt atcagctaac aacagaagat gtttcgcaat
    21901 ttttaacaag caaagaacac ttgaatgaag taatcagacg gattcaaaaa gactttagtt
    21961 gtatggaaga aatgaagtac tttattcaaa gagtggaaga acagttgtat atggaaagcg
    22021 aaacaagtca aacgaaggtc acagtcagcc ataatcattt agacgtgtat aaacgtcgtc
    22081 ctgaaacggt aaaaaaagga acagagcttc tacgactaga aacagaaaaa tacatcatga
    22141 atgtgtacct agaaaggagt gagctggtcg aaaactttgt cagctacacg atttgtagga
    22201 aacactatta ctcagaatca gaatattatt caattttcga gagcatggaa ttaatggaag
    22261 aagaaatgga agagctaaac agcaaaaatg cgacggtaga cgattattac cttgtattta
    22321 ctaggttttt gacgtgtggg acggaatcta tcgtgttaat gggaatggat tatgaactcg
    22381 ctgtacccga agcatttttg agggaatatg tagaagaagt agataaccca ggcgacgact
    22441 ggtacacgac tttaactgat tttttagaca cctacactta cgacgataca gaagaaatcc
    22501 aaaatgaact tgaccgtaaa cgaatgccat atcaacgatt tgaaaggaaa aaaggtaaat
    22561 aaccatttat accgaaaagt gaggatgtgt gaaataagaa caaaaaacct tgaaattaag
    22621 ggaatggttc ttatttcgca ccaatcgctc caaccgcccc cgccgcttag gtggtgcgaa
    22681 ataagcgggc aaagcccgat aacctaagag aaagaaggag atccatatga ataaaatcat
    22741 tcctctattg ttcgtactgt ttgttggcga gatacttatg cgtcatgctg cagctgtaca
    22801 tttaaacttc gtagtattct taggaatact ggtattttgt ttgatctttt ggcgatttgt
    22861 attagccctt gtgagtctac tattcaagat gattgcagcc atatttttca tagggttatt
    22921 tcgatgaaag gagagagtac taatgccaat tgcaatagga aataaacggt tacccgtgac
    22981 attagatgaa aagagacaaa aagaattgca gcaactaaag cagaagtacg gaaaaagtga
    23041 atctaagatt atgtgtattg cgttagatct attgatcgcg caagaaaaag caggatttga
    23101 tataccagca ctaaaaaagt gacgtcactt ttcatttgaa gaaacaaaag tgatagcact
    23161 taattgttcc tactatttag aatattttct atgtcctttt tattccattt tgtatcaata
    23221 actatgaaat ctttaaagta tccagaagag attattttta gatcaatttt tttcttacct
    23281 gattctaaag attttgtttt cttaaacata ttatgatttc ctttcgacta ttttcatata
    23341 aatgttaaca tagtttatgg attcatggaa tgttaatcta atttaataga aaaggataat
    23401 taagtatata cgttttttct tatgtatgtt atactatgta tatacagaaa gaggtgtcta
    23461 ctatggaaat gttacctata aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt
    23521 atattatgga atatcttgga attcatacag aagacaaagt gaagataata caagaagaag
    23581 taaacggtca gaagcggcta attatcgaag cggctaatat agaaaatgaa ttaacaattg
    23641 aacagttatt tgaaaattac actgatgaaa gaaatcatgt gacgattcaa aatttaggtg
    23701 aagcagtagg taatgaaaaa tggtgagaaa acctagacaa ggcgatattc tattattgaa
    23761 tacagcacct cgatctggac acgaacagac aggtaaaaga ccttatattg ttttaagtca
    23821 tgatattatt gcggattata gtaacgttgt tacagttgct ccaatttcta gtacaactag
    23881 aaactatccg ctatatgtaa atataaactc agaatatgaa atgaagacaa caggaaaagt
    23941 attattagat caactaacga caattgatta tgaagccaga gagtgtattt ttttggaaaa
    24001 agcgcatgat agtttagttg aggagttatt gatgaaagtt agaacagttt ttcaaaaagt
    24061 aagcaaataa aatagaaaca gcagcatgat caactattcg tgattgacca tgctgtcttt
    24121 ttttattcta tatacggttt gtctcgtaac attgtagtct tttgcaatct tagcgatcgc
    24181 aacacctttt tttagatcct caacgatgtt tttataaaca agacgacgtt ggggatcttt
    24241 tgcattggca ctgtatagct taggacgtcc tttataaaca ccgttcgctt ttgcaatctt
    24301 aatgccttgt gcctgacgtc gtttagattc ggtacgttct tgttcagcga tcatggccaa
    24361 aatttgaatg atcaaatctt taatgaattt atccaggaga ggattgccaa ttgcttcggc
    24421 catgatcggt agacttgtga tgatcagctg cacatttttc ttcttcaagt aattgactga
    24481 agcgatgatc tcgtcgtaat ttcttccaag tcgatcaata gcttccacaa taaaaatatc
    24541 ttgatctcgg acgaaatcca gtgcttcttg aaaaacaagt cgatgagtaa tcgttgcgcc
    24601 agattgtttc tcaatgaaaa ttctctctgc accgaatttt ttcatttctt caatctgccg
    24661 ttgttcattt tgatcgatgg atgagacacg tatgtatgca actctcaaaa tttttaccta
    24721 ctttctgcaa ctatttaaaa aacaccctaa tcatacacga gaaaaccaga tttttcaatg
    24781 tcgcggtcgt gtctagttag ggtatacctt atatagacaa aaactaatga tggaggaaat
    24841 agtatgtgtc acttttcttt ttaaatggca atattttttg taaataaatg tca
//
LOCUS       DAZKMB010000030        24390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000030 DAZKMB010000000
VERSION     DAZKMB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.030"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..847)
                     /locus_tag="ACG8V3_001095"
     CDS             complement(<1..847)
                     /locus_tag="ACG8V3_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0299648.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1071..3011)
                     /gene="thrS"
                     /locus_tag="ACG8V3_001096"
     CDS             complement(1071..3011)
                     /gene="thrS"
                     /locus_tag="ACG8V3_001096"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0299649.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3348..3527)
                     /locus_tag="ACG8V3_001097"
     CDS             complement(3348..3527)
                     /locus_tag="ACG8V3_001097"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0299650.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3574..5397)
                     /locus_tag="ACG8V3_001098"
     CDS             complement(3574..5397)
                     /locus_tag="ACG8V3_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0299651.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5675..7954
                     /gene="helD"
                     /locus_tag="ACG8V3_001099"
     CDS             5675..7954
                     /gene="helD"
                     /locus_tag="ACG8V3_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0299652.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8047..8694)
                     /locus_tag="ACG8V3_001100"
     CDS             complement(8047..8694)
                     /locus_tag="ACG8V3_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299653.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8873..9592)
                     /locus_tag="ACG8V3_001101"
     CDS             complement(8873..9592)
                     /locus_tag="ACG8V3_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0299654.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9723..10874)
                     /locus_tag="ACG8V3_001102"
     CDS             complement(9723..10874)
                     /locus_tag="ACG8V3_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0299655.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11091..11831)
                     /locus_tag="ACG8V3_001103"
     CDS             complement(11091..11831)
                     /locus_tag="ACG8V3_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0299656.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11835..12158)
                     /gene="rsfS"
                     /locus_tag="ACG8V3_001104"
     CDS             complement(11835..12158)
                     /gene="rsfS"
                     /locus_tag="ACG8V3_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0299657.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12215..12808)
                     /gene="yqeK"
                     /locus_tag="ACG8V3_001105"
     CDS             complement(12215..12808)
                     /gene="yqeK"
                     /locus_tag="ACG8V3_001105"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0299658.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12792..13442)
                     /locus_tag="ACG8V3_001106"
     CDS             complement(12792..13442)
                     /locus_tag="ACG8V3_001106"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0299659.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13452..13763)
                     /gene="yhbY"
                     /locus_tag="ACG8V3_001107"
     CDS             complement(13452..13763)
                     /gene="yhbY"
                     /locus_tag="ACG8V3_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0299660.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13779..14888)
                     /gene="yqeH"
                     /locus_tag="ACG8V3_001108"
     CDS             complement(13779..14888)
                     /gene="yqeH"
                     /locus_tag="ACG8V3_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0299661.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14885..15412)
                     /locus_tag="ACG8V3_001109"
     CDS             complement(14885..15412)
                     /locus_tag="ACG8V3_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0299662.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15592..16377)
                     /locus_tag="ACG8V3_001110"
     CDS             complement(15592..16377)
                     /locus_tag="ACG8V3_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0299663.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16370..17239)
                     /gene="accD"
                     /locus_tag="ACG8V3_001111"
     CDS             complement(16370..17239)
                     /gene="accD"
                     /locus_tag="ACG8V3_001111"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0299664.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17240..18619)
                     /locus_tag="ACG8V3_001112"
     CDS             complement(17240..18619)
                     /locus_tag="ACG8V3_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0299665.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18627..19046)
                     /gene="fabZ"
                     /locus_tag="ACG8V3_001113"
     CDS             complement(18627..19046)
                     /gene="fabZ"
                     /locus_tag="ACG8V3_001113"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0299666.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19071..19547)
                     /gene="accB"
                     /locus_tag="ACG8V3_001114"
     CDS             complement(19071..19547)
                     /gene="accB"
                     /locus_tag="ACG8V3_001114"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0299667.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19550..20785)
                     /gene="fabF"
                     /locus_tag="ACG8V3_001115"
     CDS             complement(19550..20785)
                     /gene="fabF"
                     /locus_tag="ACG8V3_001115"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0299668.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20804..21541)
                     /gene="fabG"
                     /locus_tag="ACG8V3_001116"
     CDS             complement(20804..21541)
                     /gene="fabG"
                     /locus_tag="ACG8V3_001116"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0299669.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21553..22470)
                     /gene="fabD"
                     /locus_tag="ACG8V3_001117"
     CDS             complement(21553..22470)
                     /gene="fabD"
                     /locus_tag="ACG8V3_001117"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0299670.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22577..22801)
                     /locus_tag="ACG8V3_001118"
     CDS             complement(22577..22801)
                     /locus_tag="ACG8V3_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0299671.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22854..23816)
                     /locus_tag="ACG8V3_001119"
     CDS             complement(22854..23816)
                     /locus_tag="ACG8V3_001119"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0299672.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23826..24266)
                     /locus_tag="ACG8V3_001120"
     CDS             complement(23826..24266)
                     /locus_tag="ACG8V3_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0299673.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6668 a         5432 c         4247 g         8043 t
ORIGIN      
        1 atttcgaatc cataaaatat tcttggattg cttctcgtgt ttttttcact ttttcgctaa
       61 gcttttcgtt tttagccata tagaaataaa aaatgatatc gatcgtagca aattgggcca
      121 aaagtgaatt agttgccgca cttcgattcg tgatttcttt tggtctagat gtttgtacga
      181 gtacatcggc tatcttgcta agcgggttat ttccaaattg cgtcaacgca atgatttcga
      241 tattcatgct tttcgccagc tcggctaatg cgactacatc tgcacttcta cctgagtttg
      301 aaacaagcca aagcactttc tttttctcat tgctgacaag ttgcgggagc aagacgtgga
      361 tatctttttc aaaaatgatt ggcttaccga cacgagacca tttttgcaga atatcttcta
      421 ctactaaaga agaggcgcca accccatata aaaaaacagt ctctgccgat tccaaacact
      481 ctacgacttt ttggatcgtt tcttcttcta gaatatctac tgtttctttt atcgctactt
      541 gtgcattgtt accaagtttg tttttgatag agtaaaagga ctcatttgct tctatatcac
      601 taaaaccttc atcgattcca gtctgtagtt ctgttgaaat cgtgattttg aagtccgtca
      661 gactttgaaa accgattttt ttggaaaaac gtacaactgt cggcgcactc aacccggaag
      721 cttctgctaa ttcattagct gtcattgtag ggaccttctc aatattaccg ataatgtagt
      781 tggccaactt tttttctgct gaagaataat cttccatcat actttcaata cgactaatta
      841 cgctcataag ttcctccacc cttttaatga tagtctatgt aagtatacca tatcagaccc
      901 ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa tccgcccaac agaatatctt
      961 atcccatcaa tcccacagat tctattgagc atctgtgtct ttgaggaata cctgttatct
     1021 attgttcgga ttgctttcta tctactactt ttttacagga aatttaccta ttagttttct
     1081 ctgctgtaat ttttgacttc tgcttccata gcatcaataa agatattcaa atcttctaca
     1141 gctgtttctt tacttccata acgacgtacg ttcactgttg catcttccaa ttccttgtct
     1201 ccaacaacta attggtaagg gatcttttgt gtttgagagg cacgtatctt atagcccatt
     1261 ttttcattac gatcatcaac ttctacacgc aaccccttca tttgcaaacg ttctttgatt
     1321 tcataagcat aatcgccatg tgcatcaaca gaaacaggga tgattgttgc ttgtataggt
     1381 gccaaccatg ttggaaaagc gcctttgtat acttctgtta aataagcgac aaaacgttcc
     1441 attgtagaaa caattccgcg gtggataact actggacgat gcgtattttc cccgtcttct
     1501 ccaacatatg tcaaatcaaa gcgctctggc aataagaagt ctaattgtat agttgataat
     1561 gtttcttctg taccaagagc tgttttgact tgaacatcta gttttggtcc gtaaaaggct
     1621 gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat ccatagcggc tttcagcatt
     1681 gcttgggctt tttcccacat tgcgtcatca tcaaaatatt tatctgtatt gtttggatcg
     1741 cgatagctca agcggaaacg ataatctgta atattgaagt cagcatatac tgccaccatc
     1801 aattcaagcg tacgtttaaa ttcatctttg atttgatcag gacgaacaaa tgtatgacca
     1861 tcattcaacg tcatttctcg tacacgttgt aaacctgaca aagcacctga tttttcatag
     1921 cgatgcatca ttcctaattc agcgatacga attggtaatt cacgataaga atggatcgtg
     1981 tttttgtaga ccatcatgtg atgagggcag ttcattgggc gaagaacaag catttcgcca
     2041 tctcccatat ccattggcgg gaacatgtct tcttgataat gatcccagtg tccagatgtt
     2101 ttgtacaact caacatctcc cattattggt gtatatacat gttggtaacc tagactaact
     2161 tctttgtcta cgatataacg ttcgatcgtg cgtcggattg ttgccccttt tggaagccag
     2221 aatggcaaac cagaacctac ttctggggaa accatgaaca agtcaagttc ttttcctagt
     2281 ttacgatggt ctctttcttt tgcttcttca cggagacgga tatattcttt caaagctttc
     2341 ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt gattgtttga gtttccacgc
     2401 caataagcac ctgccactga taataattta aatacttgta tacggccagt ggatggaaca
     2461 tgtggtccac gacacaaatc gatgaattcg ccttgttgat aagcagtaat cacttcttct
     2521 tctggcaatt cactaatcag ttctactttg tacggatcac ttgcaaaaat ttctaatgct
     2581 tcctgttttg aaagaacacg gcgttcgatc ggcagatttt ctttgacgat tttcatcatt
     2641 tctgcttcga ttgccggcaa atcttctgca gttacttggt tttgtccatt atctgtatcg
     2701 tagtaaaacc ctgaatcgat agctggtcca acgccaaagt ggatatttgg atagaggcga
     2761 cgcatggctt gggccatcaa atgagctgct gaatgacgga ctaagccaag tgcatcttca
     2821 tggtctggtg tgatgatctc aatacttgca tcttcctcga ttggacgagt caaatcaacg
     2881 agttttccgt tcactttacc agctaaagct ttttttgcta agctttttga aatgctttca
     2941 gcaattgcca atgttgttgt tccagactca aattctttga ctgcaccatc tggaaaagta
     3001 atttttatca taatagtttc ctccttgaaa tgttttgatt gatcagatca tcaagctgaa
     3061 aaacaaaaaa atcctagcat ctcttgaaaa gagatactag gacgaaaagt tcgtggttcc
     3121 acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta acggtccgac cgcccttgct
     3181 tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc ctggaaaagt ttcagcctag
     3241 cttttcctct cttatcgaag actctcaaat tcagttggct ttctcatcaa tcaattatgt
     3301 gtttatttaa ccactaataa atagaaaaat caagtgggaa actaaactta ttttttccat
     3361 tccccattta caccgtatgt tccttttttc atctcattga tgatgacatg gatgtgttct
     3421 tttggtgcct gcgtgttttt atgaacagct tccgtgatat ctttcatcat attttctaat
     3481 tgttccggac tgcgaccttc tactaattcg acatgtacga atggcataat gctctctcct
     3541 ctttctgatc gttctttttt caactcgatt ctatcatact atgtttgtct tgggtactga
     3601 atcaggaaaa atggctggat ctttttttgc tctcaaggca ctcaaaatag agaccgtatc
     3661 cacgacttct tgcaaaacag ctccgaataa tgcggggatt agtcccgtac ttgcaattaa
     3721 catcaaaaaa atacaaataa agatgccaat cagtacagac tgtttcgcta ttctcatcgt
     3781 atctcttgaa atcactactg cttttgctac acgcaagaga tcatctttca agatgaccgc
     3841 atctgcactt tcactagcag ctgtagaacc atgagccccc attgcaatac cgatatctgc
     3901 agcagcaagt gctggtgcgt cattcacgcc gtctcctacc atcgcaacag gtctttgatc
     3961 tttagggata ctttctaaaa tggtcagttt atcttgaggc aagctacggg catgtacaga
     4021 atctaattgg actttttctg caatcgtttg agcaatggca gatccatccc cggtgatcat
     4081 catcaaattt ttcacatcaa gttttcttag ctccttgatt gtctttgctg cttctgggcg
     4141 aatctgatcc gaaaatagga tcgttcctgc aaaagatcga tcaattgtta catagattgc
     4201 tgtattggac gtttctattt cttcagctga aacaaaagag gcacgtccaa tctttacttc
     4261 atgattattg actactgctt ggattccttg accggtcact tctttcagtt gactaacagg
     4321 atacagttgg ctgtttttca cgttgttcac taacgaacga gctaagatat ggctagattc
     4381 ctgttctgca ctagctgcat acagaagtag ctcttctttt tttattccat tgactggctg
     4441 aatcgcctct acttccaata tccctttggt aagcgttcct gttttatcaa aagcaatcgt
     4501 ttttgtacgt gcgagctttt caatcgttgt tccagttttt acaacgatcc cgtttttact
     4561 tgaacggctc attcctgcaa ctaaagcaat gggcgcagct agaatcagtg gacaaggtga
     4621 tgccactacc agtacctcag caaatcgtaa agggtctttt gagaaccacc aagaaatacc
     4681 accgatcaaa taagcaatca acgtgaaagg gactgcataa cgatcagcta aacggacaaa
     4741 tcgggcaggc ttttcttttg attcttcgac tagtttaata atcatttgat actggctgtc
     4801 tgcggcacgt ttttcagcga tcatttggat cgatccgtct ccatttaaag accctgacat
     4861 aatctgatct ccaggtttct tggtttccgg gcgggactca cctgtcaaag aagattcatc
     4921 gaccgaagat tctcctttgg tcactcgacc atctgcgggt acaagttcgc caggcttgac
     4981 tagtaactgg tcatcaatat ttatttcatc gattactata tcctgcaatt ttccttcttt
     5041 gaatcgatgg gcaattcttg gggtgttatc taacaaagac tgcagttctt tacccgctcg
     5101 tttattcgcg taatcctcta gactatctcc gcccgtaagc atgatcaata tcatcaaact
     5161 agcccagtat tctttgacgg ctaatgttgc aaggattgcg gtaatcgcta atacatctac
     5221 gccatatctt cctgaacgta acgtcttgat catttccaaa aacatcaaga aggcgagaat
     5281 ccctccagta aatgcgacaa tcgcaaatgc aagggccggc tggtgaaaaa caaattcact
     5341 taataaagct atcccaccaa taagcaaagt aagtaaaaat tttttgaaat gtgtcattgt
     5401 tttttctcct cttttatgtg acttagagag gttagtaata aatttccagc tgcctctttc
     5461 atcttaagca tagtccttga ctagcacttt ttaaaacaat aggccttaat tgaaaacaac
     5521 tatcactatc agcgtttcca aaattcatca gttctacaaa caaaaatatt cagcaaggat
     5581 tactttttcg attcatcagt taaaaagtac cttttttcga aaaactatgc tatactttta
     5641 attcgttatt tacggcggaa ggagcaaaca tgcgatgaat gagaaacaac tagaacaaac
     5701 gcacgtggat gaaacgatcg aactaatcca gatcgaacaa aatatgctgg atacaaaaca
     5761 acaagaattg actcaggaaa tgaatcattc gacaaaagac acagctaatc acaagattcg
     5821 agctggttcc aatgaagctt tttatgaatc tgctgttgaa tatcgccaac atgagcagga
     5881 attgcttttg aaataccata ctcttgaagc acagcaaaaa cgcctgcaaa cgttgtctgt
     5941 catgaaagat agtccttatt ttgcccgtat cgactttaaa gaagaccgtg aaacagaaac
     6001 attatatcta ggtatcgctt ctttaagaga tctggcagaa gaaacaatcg ttattgactg
     6061 gcgcgcacca attgccaatc tttattatga aggtgagatt ggtcccgcat tttatgaaac
     6121 tgaaacagat agaatagatg tcgatctgca gttgaaacga caatttaaaa tccaagaagg
     6181 acgtatttta tctatggtag atacgtcgga agtaatcaat gatgactttt tgctagagat
     6241 attggatgaa tcatcaagca accatatgaa aaacatcgtc tctacgatac aaaaagcaca
     6301 aaatgcaatc attcgggaca caacaagtaa agtaatgctg attgaaggaa tcgccggaag
     6361 tggaaagaca tccgctttgc ttcaacgtat tgcttttctg ttgtatcata atcgcaagtg
     6421 gctcgaggct gaaaacgttc ttctattttc acccaaccat ttgttttctg attacatctc
     6481 gactgtattg ccttcattag gtgaaagcgg cgtgcctaca cagacattca aaaattattt
     6541 ggtgcagctg ctgccagagt tttctttact agatgaacag caacaagaaa gtggtttttt
     6601 gctaggggaa aaagatccga tacaagtgat gaaaagcgga ttgaccttag tcgatcagat
     6661 tgatcgctat atccaaggca tcacaaacta cggtcccttg ttccgtgata tgaaaataaa
     6721 tggccgtacg atcctatcaa aggaatcgat tcgccaatgg tataaagaaa caaatgaaca
     6781 attaccttta catcatagac tttctctatt acaaacgaag ctcctgaaaa aattaggcgg
     6841 tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat ctggcggaag aacagctcca
     6901 agaactatac gcaacagatc caaatcttga atatacagaa caaaaggagc gttcattacg
     6961 taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt cgaatccatc gagggatcat
     7021 gaattatcaa tttgtcaata taacgaaaca atatctccac tttctacaaa ctgtgccact
     7081 ttctcttcta acacgacata aaatcagcga gaccaggtgg caggaacatt tgacaactgt
     7141 tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat gctgtactat tctttttact
     7201 gatgcgccga acatatccag tgacggttac acacaaagct cgctatatct ttattgatga
     7261 aatgcaggat ttcgcaccat cacaagtggc attgctgcaa gccttgttcc caaaagcaaa
     7321 tttgactttc tgtggagatc taaatcaaaa tgtctttgga aatgaaacga tcgttggatc
     7381 attggaagac cttttcgtgg accgtgaagt cactcgtttc caattaacga caagctatcg
     7441 ctccacaaaa gaaatcactg attttgctaa tcattttatt gtgaaagaac atcaagtaga
     7501 aacaacagca agaaaaggag accttccttt gctcatccaa ggaacaacag atgatatcaa
     7561 attgaattgg ttagcagata ccattcgaac gactcaggga aaagcccggt attggcgtac
     7621 agcaatcata gctaaaacac aagaagaatg cgaagctctt tatgctcaac tgccagacga
     7681 gttgaaacaa caagtccagc tgattttaaa tgagagtgac tttatgaagc gttccattat
     7741 tttacttcca gcctttttag caaaaggtct ggagtttgac cgagttttct tgtgggatgt
     7801 agatgcagaa catttttctt ctcagcaaga caaattgatc ctttacacca tgtgtacacg
     7861 tgcgatgcat gaattgaccc tttttacctc caataatgaa agtcctttgt taaacaatat
     7921 ggatatttca tcttatgaga aaaaagtact gtaagtaaaa agacaactgc tatcattaat
     7981 gttagcagtt gtctttttat ttacaataat aattgcagaa aaagtaattc aatcatttaa
     8041 ggtcatttat tctatatata aatccaaatc tgtttcagga tattttaatt tgacaagcga
     8101 aataatcatt ttcagttctt ccatgttttc tggatattct gggtcaaata ccttgctatg
     8161 acggccaatc ccgccattgt cgatcaatcg gtaaattcga ttccattcaa tgaattggtc
     8221 aattgattgg aaattgctga ttggatagcc gccacaatcc aaaatctctg atacacgaaa
     8281 tgctaggtat gcactatcga tcactttatt ttttttcgtg tctatcttct catttctttc
     8341 gataagaatt ttttcaggac gatgtttcaa gctccactct aatagtagaa agaaagcctc
     8401 catccgatac tcttcttcaa ataaccgaaa ggctgaatca gaacgacgaa aaagcagcgg
     8461 aatcagataa ttttgtctat aaatcagcat tggtgcgcgg tccaccatac actctaaata
     8521 acggttcaga taccctgtac cgacatagtt tcctaatcga tagccttcaa cgccgaatct
     8581 tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc acagctaact cttctacttt
     8641 gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg atttttgctt ccatattctt
     8701 ccccgtccat cttccacata cttcatatca tttcacatga tacttatgct ctcatcatac
     8761 cacaggttga attagaaaag aaaataaatc acacaaaaaa gcaagacaat ctgttagtga
     8821 gattgtcttg cttttcgaaa aagactgaat acttttcgtt aaaactggtt gcttagtctt
     8881 ctaatgccac attatggaac acatgctgta catcttctag atcttccagt gcatcaatca
     8941 ttttttcaaa tgtttcttga tcttctggag atagtgttac ctcgttttga gggatcattt
     9001 ggatctcagc gattgaaaaa tcagtgattc ctttttcttt taatgcttct tgtatactat
     9061 ggaaatccgc tacttcaccg taaacgatga tttgtccttc ttcttccatt acatcgcgaa
     9121 catcgatgtc tttttccatt aggtattcaa ggatctcgtc agcgtcttct cctgcaaaag
     9181 caaagattgc tgtattgtca aacatgtaag aaaccgctcc ggaaacaccc atgttcccgc
     9241 catttttccc aaatgctgcc cgtacgtctg ctgctgttcg attcacattg ttcgtcaatg
     9301 tatcaacaat caccattgag ccgcttggtc caaatccttc atagcgcaat tcagagtatg
     9361 tttcatctcc tgcacctttt gctttttcga ttgctcggtc aataatatgt tttggtacat
     9421 tataagtttt agcgcgatca atgacgaaac gtagtttttg gtttgcatgc ggatcaggat
     9481 cacctgattt tgctgccgcg tagatttcaa tcccaaattt tgcataaatc cggctattat
     9541 tagcatcttt agcagttttc tttgctacga tatttgccca tttacgtccc atattttcac
     9601 ttcgctttct gatttgatag tttcataacg caaaaacgtc ctgttccatt atacttgtga
     9661 atcacgattc tgtgaagctt tttttctttc ttccttcatt cttctcattt ttagtcaatt
     9721 atctatactc ggattgggat ttgcccgaaa ttctgttccg gaatattgcc tcccaattga
     9781 tagatttgat cacttctaac taacagttcc gcttcttgtg cctctttttt tccgattcgg
     9841 gaataaatag aaatctcttt tttttctttt aagaatcttc ttccagcatc ggtaaatcca
     9901 agtagtcgaa gataattttt attttgttgg acaggaatct cttgcttttt gatattcagc
     9961 aagacataac aagataatcg ttggatccgt gtccatgtat atctttttgt ttttaccttt
    10021 gacacaaaat cctcaaaact atcagcagtt gcagctgctg ttttcatgcg cgattctatt
    10081 ccttcggtca tctgatagat atctgtcagt tccagcaaag tagaagataa caaccgataa
    10141 cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac aagttgaaag atcttcttct
    10201 gtctgaggcg gcacaagtcc agctattctt tttccttctt ctaacgcatg gcgaatggct
    10261 gttgcactgg ctatcctctg atctgtgatg attttttcat gatagcctgc acttttacgt
    10321 cggatcggat gtaattccat agggtggaca tacttggcat tttcctttgc ataacttaat
    10381 cccaagatat gatttggcga agaaaaatca agcgtaatat cgggatacag actgcgaaaa
    10441 acctcagtca ttttttgcgg ataactatac gtttgatcag acagttcttg aaatgcctct
    10501 tctatcctat cttgattctc ttggacaaag cgtccgaaac tgctgtaatc aaacgaagtc
    10561 tcttcttctg ttccaaaaca caaactgtca cagtccagtg tttgcaagat ttttactgca
    10621 cctttagcaa aatagtcagc tgactgaacc gcccattcga cgggaagctc cactactaaa
    10681 tccacaccat tttgtaaagc ttcattcgcg cgatgccatt tatcgatgat agccggttcg
    10741 cctctttgca aaaaattccc gctcattaca gcaatgacga cctctgctcc tgttattttt
    10801 cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag gattgtactc tacgataatc
    10861 ccgcagcttt tcatgcgcat cctccttcat agttatcgat aaaaaagaaa tagagacaag
    10921 taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat aaactattct cgcaaacaca
    10981 atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc tatatcgttc ggatatataa
    11041 tttatgttga agatttattc ttttcttgtt aactcgagca ggcaaaaccg ctagtctttg
    11101 tatgctacaa agaaccagcg ctttgatttc tctgatgctg cttcgtctgt aaaatcagaa
    11161 aatgcttcga ctttcgaaaa gccgctgttt tccagcattc ttaaataact ttccaatgga
    11221 taggtacgct cttgatgtag ttcatcttca cgaataaatc gaccagaccc ttcttgttct
    11281 tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct cgcttcctgg ataactatcc
    11341 cacaaaaaag caaaattatc cccttgataa tgatagctat attctggaaa aacctcatct
    11401 acttgatagg tggagtgtac gtcaaataaa aatcggccat tttcttctaa cgcttgatag
    11461 acttcatcaa acacttgctg tacctcttgt ctatttttca tatagcacag cgaatcagaa
    11521 aaacaggtaa ttgcctgata ttgtccgatt tctgataaat cgagcatatt gccttccaca
    11581 aattggatcg atgcgtcttc ttcatatgct cgctggctgg caatcatcaa catctcttct
    11641 gaaaggtcta accctgtcac tttatatcca tcttttgcaa aggcaaccgc taatgctcca
    11701 gtaccgcatg ccagttccaa aatatttttt gtgtcttctt gtaaatgacg tttcgaaaaa
    11761 gctaaccatt gctgataaag tgtttcgtcc atcacttcat cgtaaatgaa agcaaatgtt
    11821 tcataagcca tatgttaatc caaccattca ctaagatcga ctagcggagc atctgaccat
    11881 agtttttcca gattatagaa atttcgttct ggcgcatgga aaacatgtac gatcacatct
    11941 ccaagatcga tcaataccca ttttccgcct tcttttcctt caatacgttt tacttcatat
    12001 ttgttttctt cttctttatc aatgatttct tctgtaattg cgttgatttg acgttcgcta
    12061 tttgctgaac agatcatgaa ataatctgcc aacaaggaaa catttcgaac gtctaaagcg
    12121 ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg caatttttaa ttgttcttta
    12181 ctatctatga tgatttcctc ctaatatgag cagcttattt ttttgctacc cagtaattat
    12241 atgtttcgat tgtttttgga taaatctgtt gttcttgcgc aagcaaatgg gccaatgtat
    12301 gttttgtttc aaatgctaca gcttcgtcta gatcagccca tgcaatggct cgtgcatctt
    12361 gtaccccagg aaaatctcga cctggttcaa tatagtctgc tacataaatg attttgtcaa
    12421 ggagactcat cttagcagcc ccagtcgtat gcacccggat tgcatgaaga atctctgcat
    12481 cggtgattcc taattcccgc tctacaaacg aagcgccaac aagaccatgc cagattgcat
    12541 tattataatt caataactcc ggatcaaaac cgtctcttac gattaccatt ttgaactcct
    12601 catctggtcg ttcttttgca taatcatgtg tcaatgcagc aatactagct ttttcaggag
    12661 aagcaccgta ttttttggca agagccacgg ctgtttcttc tactccaagc acatgcttga
    12721 agcgttgttc gctcattctc atctgtattt tttgcatcag gttttctcta gaataagcag
    12781 tatattcatt actatagtcc atattcatac aacccttcat tttgaatata atcaattact
    12841 ttatctggaa caaggtagcg gatagaacag ccttctttga tcttttgacg tattttcgtg
    12901 gaactgatgt cgatctcagg tacatctacc caaattacag gatatggagt atccgttgtg
    12961 tatcctggtc gacgtatacc cacaaagttg accatagaag tcagctcatc gattttatac
    13021 cattttggca aatattctac catatcccca ccgataataa agtaataatc tgtatccgga
    13081 ttgttttgcg tcaattcctt catagtatca taggtgtaac ttttcccacc acgagctaat
    13141 tcaatcgttt caatctgcaa aaaaggattg tcttccaccg ctaattccaa catgttgagt
    13201 cgatgttttg catcaatcgt ctgtttctca tcaacatgag gtggctgata agaaggcatc
    13261 aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt cagccatcac taaatgtgca
    13321 aggtgaacgg gattgaatgt tccgcccaag atccctactt gctttttctt ctctaagaat
    13381 tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg tcttagcttg cgtattcatc
    13441 atggtcactc cttatattgc ttttacttct tttgatagcc tttgatactt ttcttttgtg
    13501 gaaggtttgt ataatacaat aacacgtcca atgatttgga cgatttcgca atctatttct
    13561 ttttctaata cttgagctgc ttcttctgca atctcatctg tattttgaag taacgatact
    13621 ttgatcagtt ctcttttttc taaagcttct tcaatttgaa cgatcattgc tgaattgatc
    13681 ccaccttttc cgatttgaaa aatcggctga agatgatgcg ctttgctacg taaaaaacgc
    13741 ttttgttttc ctcttaaact cataattttc cttcttcctt aaatcaatgc tttgcgtttc
    13801 aatacatcta caccctttgg cgcccagccc gcaattacta ctggcgctgt tacagtgatc
    13861 cagcctagac cggcaaaaac gatgtctgtt ttttctttca cagaaaactc aaaacgaaca
    13921 agctccggaa atgcctcgac ttcatcagga cgaggaggct gtaataatcc acctacatgt
    13981 ttttgataaa attcgtctgc tttttccagt ttcgttcgat gaatatttac atcatttgac
    14041 acataagcaa taaatgaagc acgattgccg ctgataaaat caaatcttgc aagtccgccc
    14101 aagaaaagtg tttgcccttc attcagctga taaactttag gtttgatttc ttttaacggt
    14161 gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca tttgatggcg atggataatt
    14221 cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa tctcgatttt atccaaggtc
    14281 gttcctggaa actgcgaagt tgtgattaca tcttgtactc ccgctgtatt tttgatgatt
    14341 tgattgataa gcgtcgattt tccgacattt gttactccga cgatatagac atcccgtcca
    14401 tcgcggtatt cttcgatttt ttctaatagt tccgtcatct catgagattt cttagcacta
    14461 gttaataata catctactgg acgaattcct gcttcgtaag cacgttcctt catccattgt
    14521 gttaatttag tttttttcaa tgatttaggt aatatatcca ctttgttccc aactaaaagt
    14581 actggattgt caccgacaaa acggtgtagt cctgggatta gtgaaccatt gaagtcaaaa
    14641 atatccacca cattgacgat caatgcatct ttttgcccta attcattcag taagcgcaaa
    14701 aagtcgtcat ccgtcaatga gacatcttgg gtctcattat aatggcgtaa tctgaaacaa
    14761 cgctgacaat agacttcccc ggaagctaaa cctttttcca aagccgtttg cggcgtgtat
    14821 cctaattcac cgggtttgtt tgtttgtata acggcaccac agccgataca ttgtaattct
    14881 tctgtcattc gattccacct cgccagccca tatcagggtg ttttgctaat agataggcca
    14941 taattctttt ttcccaaaaa cgattaaatt gtgtcttcca actatctgtt gcgataatgg
    15001 gttttacaag gatgctacgt atccctgcac gattggctcc acgtatatca gtcataatct
    15061 gatcgcctac cattactatc tcatcatctg ctaaatccaa ttgtttcttt gctatgttga
    15121 tccctcttgc taaaggtttc attgctcgtg caacatacaa aagatcaaat ttttcaatcg
    15181 ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac aacagggatt cctgcatttt
    15241 tcatttctaa aatccaagta agcaactctt ctgttccatc tggattattc catgcaatca
    15301 acgtattgtc taaatcggtc aatactgctt tgatgcctag attctttaat tgggcaggtg
    15361 taatctgata gatggcttct accatccatg ttggtttgta atttgaaaac atgctgtctc
    15421 ctcatctgca aaaaatgaag gagcacataa agtactcctc aaccccgtat tatactgcat
    15481 atcagcaaaa aaaactacct ctatactcgt tcgtctttca gaaaaagcat ttctttacaa
    15541 aatgaccaaa aataaagatt gatcacagaa aaatttagaa acaaaaaaca actaatattg
    15601 gcggaacctc ttgtatcgtt tttccagtaa tttatgctga tctaatcgaa caagttccgt
    15661 caatctttca atcaacgctt ttttcataat gtgtgcgact ttatatcgtg gcaactgctc
    15721 gccgttgaac gtttcaggaa taactcgatc aacgattcct aactcgcgta gttctggcgc
    15781 agtaattttc atcaactcag ctgcttctgc ggcgcgacta ccgtctttcc acaaaatcga
    15841 cgcaaatcct tctggagaaa gcacagcata aatcgtttct tccagcatcc agacttcatc
    15901 cgctaaacct agagcaaggg caccgccact tccaccttca ccaatgatga tggcgatgat
    15961 cggtaccttt aaatcagaca tttcgagcag atttctggca atagcctctc cttcgccgcg
    16021 ctcttctgct cctacaccgc aatatgcacc tgctgtattg atcaaggtaa tcaccggtct
    16081 attgaacttt tccgcttgtt tcatcagtcg taaagctttt cgatagcctt caggatgggg
    16141 agaaccaaaa tttctgcgga tattttcctg cagatcccgt cctttttgaa taccgatcac
    16201 tgttaccggt ttgccatcta atcgagcgat tccaccaaca atcgcttcgt catctgcaaa
    16261 taaacgatca ccatggcatt cctcgaaaga atcgaagatc tcttcaaaaa aatccaagct
    16321 tgtccatcgt tctttagctc ttgctaactg aacgacatca tgtgctgtct tatccacgat
    16381 ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg gattttctgt ttcaattcca
    16441 tgcggggaac aatctgatcg acaaaaccat gagataataa gaattcagcc ttttggaaat
    16501 cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg tcttccagca aaaccgatca
    16561 agctttgggg ttctgctaga ataatatcgc cttccattgc aaaactggca gttactcctc
    16621 cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc ttcattgcta tgccgtttga
    16681 cagcagcaga gatttttgcc atctgcatta gagaaaagat tccttcttgc atccgtgcgc
    16741 cacctgaagc tgtgaacaag acgactggaa gactttcctt tgttgctcgt tcgaataatc
    16801 tggtgatctt ttctccgaca atcgttccca tacttcccat gataaaatta ggatccatca
    16861 ctccaataat gaagggaatc ccctgaatgg ttgctttacc agtgaggact gcttcatgaa
    16921 gtcctgtttt atcttgcatt tttttaattt tttccgtata ttcgggaaat gctagcggat
    16981 ctttggttac aagatccgta tcccattctt caaaactttt ttcatcaata atcaaagcta
    17041 gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg agggcatact ttttcttcac
    17101 cgatgtcttt cgtataaagg atatgtttgc aatttggaca ctttgcccac atgttatcag
    17161 gaacagatgg cgagttagca ggtttgctct ctctattagg attgatccga atatatttct
    17221 ttttcttaaa cagagccatc tatgcgcctc ctatctccgt ttctggtgtc caattcggta
    17281 aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc tatgactgca ggatgactaa
    17341 tcaaatccat ctgaaattct gcgttcgtga tgattccttc tgtgataaat tcacttaagg
    17401 ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc atggacgatt atttttgcaa
    17461 tcattgaatc ataatatggc gggatcgtat atcccgggta aactgcactg tctactcgca
    17521 gtcccatccc acctgctgga agcagtaagt tttggatctt ccctggagaa ggagcaaaat
    17581 ggaaagcagg attttctgca ttaatccggc attcgattgc gtgtccttgg aatactactt
    17641 cttcttgaca cacattcaaa ggctcaccgg ctgctatctc cacctgtttt tttactaaat
    17701 cgattcctgt gaccatttct gttactggat gttccacttg gattcgagta ttcatttcca
    17761 tgaaataaaa atcgccttct tcatccatca gaaactcgat cgttccggca ttttcataat
    17821 tgactgcttt agctgctcga acagcagcgt ctcctagtgc ttctcttttt gtttgtgaga
    17881 tcactacaga aggagattct tccagtactt tttgattgtt gcgttgtaaa gaacaatctc
    17941 gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa gatctgaact tctatatggc
    18001 gagcaggata aatgattttt tccaaataca tatcatcatt tccaaatgcc gctgctgctt
    18061 cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt tgattgaact ttccgaatgc
    18121 ctttcccgcc accacctgca gctgctttca gcatcactgg ataaccaatt ctgtctgcta
    18181 tttccaatgc ttcttctact gtagataata caccagtact tcctggaatg actgggacat
    18241 ttgcttcttg catcaattga cgcgcattga tcttatttcc cattgcatcg atcgttgcac
    18301 ttttaggacc aataaatgtg atattgcact cttcacacat ggaggcaaat cggctatttt
    18361 cagataaaaa accaaatcct ggatgaattg cttcggcttt tgtcacgatt gcagcgctca
    18421 gtacttcctg aacattcaga tatgaatcaa cggcttttgc cgggccaatg cagattgctt
    18481 catctgctaa ctgtgtatga agtgcttcgg catctgcttc tgaatatatc gctacggttt
    18541 ggatccctag ttcgcggcat gcacgaatga tacgtacagc gatttcaccg cggttagcta
    18601 tcaatacttt agaaaacata tttcacctat ccaatcataa aggttaattc cgcttcggct
    18661 acttttttac cgtctacttt agcgattcct ttgcctattc cggctgaagc tttcactttc
    18721 aatatctcaa cttcaaggta taaggtatct cccggtgtaa ctttcttacg gaattttgct
    18781 ttgtccaatc cgccgaaata agccgtcttt cccttgaatt gttcaagaga aagtaatgca
    18841 actgctcctg cttgagccat tgcttcaaca atcaataccc ctggcataac aggttcttct
    18901 ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat ttttcttagc aacgatccgt
    18961 tctccttcaa ccatttcttc cactcgatca atcaaaagca ttggataacg atgtggaatg
    19021 atttctttta tttcttgtat gttcattgtt ttccctcttt cctgctttgc ttatttttgc
    19081 tgaatcctaa agagtggctg tccatattcg acaactgact cgttttctac aaggatttct
    19141 gtgatcactc cgtcggcctc actagttatc tcattcatca gtttcatcgc ttcaataatg
    19201 cagataactt ctcctttggc aacttgatca cctacttttt tgaaagctgg tttgtcgggc
    19261 gctggttgca gataaacgat tcccacgatt ggcgaaacaa cctcgattcc atcaaatact
    19321 gccgattctt tttctttcat tatttctgga gcagttgttg cttgttcagg tatgacattg
    19381 actggctccg aaactggcaa cgtttcgttt gtttgaacca tattcatgcc tcgacttgtt
    19441 ttatttttat tcatatacaa ttcaaattga ccttcacgca aatcgaattc cgttagcgag
    19501 gattgatcaa attgactgac taattctctg atttcattga tatccaaaat tattcctccc
    19561 agcgtttcat gcacagaacc gcattatgtc cgccaaaccc taatgaatta ctcattgcat
    19621 aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt caagtcaatc tcaggatctt
    19681 gtttttctac attgatcgta ggtgggataa attgatgttc taatgccagc aaagttgcca
    19741 aggcttcgat cccgccggct gctcccaata aatgtccagt catcgatttc gtgctgctga
    19801 cgtaagtatc tctgaaattt tcacctaatg cgatttggat tgcttttgct tctgcctggt
    19861 cattggctgg tgtacttgtt ccgtgagcat tgatatatcc gacttgttcc ggctgtacac
    19921 cagcttcttc catcgcaagc ttcattgctt tagatgcacc agaaccatct ggagtaggag
    19981 ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac gatttctcca tagattttgg
    20041 caccgcgagc ttgtgcatgt tctaaagatt caagaactag aacgcctgct ccttcaccca
    20101 tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc acgattagga tcagttgatt
    20161 ctgagagtgc tgttaaagct gcaaatccag caatccccat ctcacaaatc gttgcttctg
    20221 ttcctccagc aagaatgaca tcagaatagc catgtttgat gttacggaat gcttccccaa
    20281 ttgaattcgt tccgctagca caagctgtta cgatagctgt acaaatacct tttgcaccta
    20341 cacgcaaagc aatatttcca gctgccatgt tggcaatcgc cattggtacg aacattggag
    20401 aaactcgttt tgcacctttt tcatgtagac gaataacttg gttttcgatt gttggaagac
    20461 caccgatccc agatccgatg atgacgccaa accggtcttg attcattttt tctttatcta
    20521 aaccgctcat ctccattgct tctaaagcag cgtagattcc atagatcgaa aacatatcca
    20581 ttcgtttgct atctttttta ataaaatatt tatcgtaagg gaaatctttg acttcacccg
    20641 ctaaggtaat ccctgtttct gaagcatcaa atttagtgat cggtgcgatg ccattcgtcc
    20701 cctcttttaa gctattcaaa aagctgtctg cgtcatttcc aataggtgaa gtgactccat
    20761 agccagtaat taccactcga ttcatttgtt tgctctcctt ttcttatccg tgcatgacca
    20821 atccgccatc gacattgatg acttgtccgg tgatatatgg attttgtgct aagaaaactg
    20881 ctgtttgtgc aatatcttct acttgtccga aacgctgcat cggaatttgc ttttccgctt
    20941 gttccttgac tttgtctgct aaaacttccg tcatatcagt tgtgatgaat cctggcgcaa
    21001 tggcattaca tgtaatgcct ctagttgcag cttctcgtgc cacagatttt gttaatccta
    21061 ctacgccagc tttactagct gcataatttg cttgaccaat atttccaatc aacccagata
    21121 cactagagag attaatgatg gccccttctc gctgcttcat catttttttc aatacgtgct
    21181 gcgtcatatt gaaggttcca atcaaattga tatccagaca ttttttgaaa tcttctgcat
    21241 ccattcgcat cacaagtttg tcatttgtga ttccggcatt gtttactaac acatggatcg
    21301 atccaagctt ttcttctgct tctttgatca tttgtcccgc tttctcataa tcagagatgt
    21361 caccggaaac tcctacgcat tttacaccga atgcttcgat ttcttctatc ttttcttttg
    21421 gaatctctcc gcgaccattc aaaatgatat ttgctccagc ttttgcaaaa gctaaggcca
    21481 tcgctttgcc gattcctctt gtactacctg tgataaatac attttttcct tttacatcca
    21541 taacgttcct ccttatagta ttgctattgt ttcatcaaat gtctgcttat tctcaacacg
    21601 cagaactggt attgttttat cgattttttt cataaatccg cttaatactt ttccggggcc
    21661 gacttcgatg acttgttcga tgccgattgt ttttaactta tggatactct catagaaacg
    21721 aacaggttgc atgacttgct gttcaagtag tcccgcgatt gtttctttct tcattatttc
    21781 cgttgttgtg ttgcttatga taggaaaaga aggttcagag aattgaatct gacttaggtc
    21841 cttcgccagt ttttttgctg ccggttctaa aatagctgta tgaaaaggcc cgctgacatt
    21901 tagagggatc attcttttga accctttttc ttttaatagt aaaacagctt cgtctactgc
    21961 tttttcttcg ccaccaatca cgatctgctg aggtgtatta taatttgcag gagatacgat
    22021 gccgtacttg ctagcttcat gacagctttc ctcgatcgtt tcaatcggcg cattcatcac
    22081 tgcaaccatt ttcccgcttc cagctggggc agcttctgtc atataagcgc ctctttttgc
    22141 tactaacgca actgcttcag tgaaggatag cgcattacta gctactaatg ccgaatattc
    22201 tcctaaactc agtcctaaag ctgcatctgg gatgattcca tgctccttca ataagcgata
    22261 atacgcgatg ctgactgtta gaatagctgg ctgtgtatat tgcgtctgat ccaaacgttc
    22321 attttctgta aagcaaagtt cagccatatc gtagccgaga atctcacttg cttcatcaaa
    22381 tgtctgcttt acaacggctt cttcatataa atcttttccc ataccttgat attgtgcacc
    22441 ttggccacta aataagaatg ctgttttcat gtttttctcc aatatatcca gaatatatgt
    22501 ttgattcttc ctattcaaaa aggccgagac attcctattt caggaaatgt cccagccccc
    22561 aacctaattt taatgattat gcagtttgtt tttctacgta agtcactaaa tcttgaaccg
    22621 ttttgattcc gtcttctgtt tcgattttca cgtcaaattc atcttcaatt tcattgatga
    22681 tttggaataa atccaagctg tcagcttcta agtcttcttg gatgtcagta gtcaatttga
    22741 tttcttcagt ttcttttcct aattcttctg cgatgatttc ttgaattttt tcgaatacca
    22801 taataatata ttcctccgat attgtttgct ttttattttt ataatttaac agtctacaac
    22861 tccatgagaa tacttcccca tgttaaacct ccgccatagc ctgtaaatat tactttctgc
    22921 tgttttccta aggtgatctt tccactagaa actgcttcat ccaataagat agggatgctt
    22981 gcagcagatg tattcccata tttatccata ttggtcagga acttttcttg cggcatcttt
    23041 acttttcggg caattttctc aataattctg aggtttgcct gatgcaaaag cagataatca
    23101 actggtttgt ctttcatgac ttcttggata ttgtttgcaa cgtctcgtac ggcaaaatca
    23161 aaaatatctc tgccagtcat ttgcaaataa tacgaacctt ctgaatcttc tgaatagaac
    23221 ggatttttat tttgctggta gccagcggtc aatgagctac ttctggtacc gtcagctgct
    23281 aatttttctt tcagcaattt tttctgtgga ttcgcttcta agagaatccc acctgctcca
    23341 tcgccaaaca acacagcagt cgtacgatca gaccaatcga ttagcttaga tagtgtttca
    23401 ccaccaatca ccagtccctt agttgagccg cagcggatca ttttttcagc catgcttaac
    23461 gcataaacaa atcctgagca cgcagcactg atatcaaaag cgatagcatg agttgctccc
    23521 aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt aatcgggcga cattgttgct
    23581 actaaaataa aatccaaatc tttagggttt agatttttct gttttatcaa tttttccgcc
    23641 actaaaatgc ataaatcaga agtctcctga tttgttacac atcttcgttc acgaataccc
    23701 gtccttgacg aaatccattc atcagaggta tccatcatct tggaaagatc attattagta
    23761 ataatctttt caggaaggac gctggctgtt gcagtaatca ttccaaattg ttccattaaa
    23821 tatcctcact tatagtcttg caagaagtcg tgcaagtttt tcagtgcatc cagcaaggca
    23881 tgctgttcct ccgtagccat tccgtctaag atgttcttga ccatttcacg atgaaaatgc
    23941 tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc ccaactttac aacgcggcgg
    24001 tcatcttcac tgcggatacg ttcgacatag ccttttttta ctaacttgtt aatagcagtg
    24061 gtcaaggtcc caacagtaat tgatagttct ttggctactt ctgaagttgt ttttttcttg
    24121 tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg acagatcttt gaattgagat
    24181 tttttcagct cagactcttc gattgtcaaa atatcattga agacactaac aagataatca
    24241 tttactgttt ttaaatctgg ttccatgttc caccgtccac taattagttt gattatcaaa
    24301 tcttttgatg ttcaaactat atcttaactt ttttcaaaat gcaaccttaa aagatcattt
    24361 ttttactata tgagcttttt ttaagatttt
//
LOCUS       DAZKMB010000031        24274 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000031 DAZKMB010000000
VERSION     DAZKMB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.031"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACG8V3_001121"
     CDS             complement(55..789)
                     /locus_tag="ACG8V3_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0299674.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACG8V3_001122"
     CDS             1116..1526
                     /locus_tag="ACG8V3_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0299675.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACG8V3_001123"
     CDS             1519..2220
                     /locus_tag="ACG8V3_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0299676.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACG8V3_001124"
     CDS             2220..3806
                     /locus_tag="ACG8V3_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0299677.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACG8V3_001125"
     CDS             3825..4046
                     /locus_tag="ACG8V3_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0299678.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACG8V3_001126"
     CDS             4043..4804
                     /locus_tag="ACG8V3_001126"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0299679.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACG8V3_001127"
     CDS             5184..5693
                     /locus_tag="ACG8V3_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0299680.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACG8V3_001128"
     CDS             complement(5768..6307)
                     /locus_tag="ACG8V3_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0299681.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8V3_001129"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8V3_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0299682.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACG8V3_001130"
     CDS             7320..8171
                     /locus_tag="ACG8V3_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0299683.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACG8V3_001131"
     CDS             8199..8834
                     /locus_tag="ACG8V3_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0299684.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACG8V3_001132"
     CDS             8854..9627
                     /locus_tag="ACG8V3_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0299685.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACG8V3_001133"
     CDS             10035..10832
                     /locus_tag="ACG8V3_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0299686.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACG8V3_001134"
     CDS             10810..11223
                     /locus_tag="ACG8V3_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0299687.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8V3_001135"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8V3_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0299688.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8V3_001136"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8V3_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0299689.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACG8V3_001137"
     CDS             16000..16560
                     /locus_tag="ACG8V3_001137"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0299690.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8V3_001138"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8V3_001138"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0299691.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACG8V3_001139"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACG8V3_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0299692.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8V3_001140"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8V3_001140"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0299693.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8V3_001141"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8V3_001141"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0299694.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8V3_001142"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8V3_001142"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0299695.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACG8V3_001143"
     CDS             21907..22485
                     /locus_tag="ACG8V3_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0299696.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACG8V3_001144"
     CDS             22537..23019
                     /locus_tag="ACG8V3_001144"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0299697.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACG8V3_001145"
     CDS             23136..23510
                     /locus_tag="ACG8V3_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299698.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23885..>24274)
                     /locus_tag="ACG8V3_001146"
     CDS             complement(23885..>24274)
                     /locus_tag="ACG8V3_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0299699.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT         7906 a         4353 c         5198 g         6817 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgggagcgt caataatttt gtgtaaataa attgtcctcc tgcaaaataa
    23881 ttagttactc agtaaacatt gaaactaatg tatcggttac ctgttgaaaa cctttatggc
    23941 ttctgtttag aaatttttga ttgtatgtat caaaaatgct gactagaaag cgttctagtg
    24001 attcttcatt ttgaaactgc tcttttctac ggctgtatct tttaatttgc ttattgaaag
    24061 actcgattag attggttgag taaatggttc tacgaatgct aggtggaaaa tcataaaaag
    24121 ttaataagtc ttggttttct atgagtgact gcgtcacttt aggatagttt ttcttccatt
    24181 tctcaatcat gccggataag aaggtattcg cttcttcttt tgagttagct tgataaacag
    24241 ccttaaagtc atcacagatt tcttttcggt cttt
//
LOCUS       DAZKMB010000032        23780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000032 DAZKMB010000000
VERSION     DAZKMB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.032"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            373..1812
                     /locus_tag="ACG8V3_001147"
     CDS             373..1812
                     /locus_tag="ACG8V3_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0299700.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2107..2325
                     /locus_tag="ACG8V3_001148"
                     /pseudo
     CDS             2107..2325
                     /locus_tag="ACG8V3_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            2574..2789
                     /locus_tag="ACG8V3_001149"
     CDS             2574..2789
                     /locus_tag="ACG8V3_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299701.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3006..3262)
                     /locus_tag="ACG8V3_001150"
                     /pseudo
     CDS             complement(3006..3262)
                     /locus_tag="ACG8V3_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3449..4360)
                     /locus_tag="ACG8V3_001151"
     CDS             complement(3449..4360)
                     /locus_tag="ACG8V3_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0299702.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4379..5383)
                     /locus_tag="ACG8V3_001152"
     CDS             complement(4379..5383)
                     /locus_tag="ACG8V3_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0299703.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5380..7503)
                     /locus_tag="ACG8V3_001153"
     CDS             complement(5380..7503)
                     /locus_tag="ACG8V3_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0299704.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7508..9955)
                     /locus_tag="ACG8V3_001154"
     CDS             complement(7508..9955)
                     /locus_tag="ACG8V3_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0299705.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9942..10331)
                     /locus_tag="ACG8V3_001155"
     CDS             complement(9942..10331)
                     /locus_tag="ACG8V3_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0299706.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10391..10894)
                     /locus_tag="ACG8V3_001156"
     CDS             complement(10391..10894)
                     /locus_tag="ACG8V3_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0299707.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10907..11131)
                     /locus_tag="ACG8V3_001157"
     CDS             complement(10907..11131)
                     /locus_tag="ACG8V3_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299708.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(11235..13145)
                     /gene="ltrA"
                     /locus_tag="ACG8V3_001158"
     CDS             complement(11235..13145)
                     /gene="ltrA"
                     /locus_tag="ACG8V3_001158"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0299709.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(13891..14028)
                     /locus_tag="ACG8V3_001159"
     CDS             complement(13891..14028)
                     /locus_tag="ACG8V3_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0299710.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(14025..15209)
                     /gene="mobT"
                     /locus_tag="ACG8V3_001160"
     CDS             complement(14025..15209)
                     /gene="mobT"
                     /locus_tag="ACG8V3_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0299711.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(15468..15758)
                     /locus_tag="ACG8V3_001161"
     CDS             complement(15468..15758)
                     /locus_tag="ACG8V3_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299712.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(15748..16890)
                     /gene="dcm"
                     /locus_tag="ACG8V3_001162"
                     /pseudo
     CDS             complement(15748..16890)
                     /gene="dcm"
                     /locus_tag="ACG8V3_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(16966..17130)
                     /locus_tag="ACG8V3_001163"
     CDS             complement(16966..17130)
                     /locus_tag="ACG8V3_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299713.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(17123..17797)
                     /locus_tag="ACG8V3_001164"
     CDS             complement(17123..17797)
                     /locus_tag="ACG8V3_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0299714.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(17875..19215)
                     /locus_tag="ACG8V3_001165"
     CDS             complement(17875..19215)
                     /locus_tag="ACG8V3_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0299715.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(19298..19633)
                     /locus_tag="ACG8V3_001166"
     CDS             complement(19298..19633)
                     /locus_tag="ACG8V3_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0299716.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(19643..20017)
                     /locus_tag="ACG8V3_001167"
     CDS             complement(19643..20017)
                     /locus_tag="ACG8V3_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0299717.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(20030..20344)
                     /locus_tag="ACG8V3_001168"
     CDS             complement(20030..20344)
                     /locus_tag="ACG8V3_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0299718.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(20457..23684)
                     /gene="fss3"
                     /locus_tag="ACG8V3_001169"
     CDS             complement(20457..23684)
                     /gene="fss3"
                     /locus_tag="ACG8V3_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0299719.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         6646 a         5141 c         4102 g         7891 t
ORIGIN      
        1 ctatggaatt gaatatcata gcaatgtatt tgaaaagcaa attggaaaac aaataggtgc
       61 aacaccataa atatgaaaaa attgggaaaa agtagaatgg aaatttgctt acgtctcacg
      121 agatagctca cactattaat ctgaagaaca atgaagtcga cttttagaaa ttgctagaag
      181 aaagtaaagc gaattatcac tagttactat agagtaagtt caaataaagc atctccaact
      241 tacatggtta catgaggata tcaaatcgaa attactcaag cttcctatgg ctagagtcgc
      301 gagaagttgt tgggcagtag acataggtta tattgttctt agctacacga tcattagaaa
      361 ggaaatccta ttatgtatag ttatcaagaa gatgcaaaag tccaaaaaaa tcgctggtgg
      421 attttgattg ctatatctat gtttaccttc atgtccacat tggatggcag tattgtcaat
      481 attgcactac ctaccatctc agatgatatg ggcataccaa tgaatcaagc ggaatgggta
      541 gtgtccgttt atctgatgat tgtctgttct tgtttgttgc tgtttggtaa aattggcgat
      601 agccgtggga aaatcaaagt atttcggatt ggtacgctga tctttgtctt gggatcgctg
      661 ttgtgtgggt tcaatcattc gctaggtatt ttattgttcg cacgggtggt acaagccatt
      721 ggtgctagta tgactatggc tacaaatgcc ggaatcatca cggaagtctt tcccatacat
      781 gaacgtggtc gggcattggg agcaataggt gcgtttgttt cattaggctc gattgctgga
      841 ccaggtcttg gcggcttgat tttgtcccaa ttttcttggg cgtacatttt ttggatcaat
      901 gtaccggtgg gaatcgtaac gatgttgatt gctgaaaaat tcttaccaaa agatatcacc
      961 atgagcggcc atacaatcga tggagggggc tttgctgcca ttgcggctac gattatcagc
     1021 ttttttggag caatcttttt ggggcaagaa agtggctttt tccatccagt cccccttgta
     1081 ctgttcgtaa tttcagtgat tgcctttgtc agctttttaa aagttgaaaa gaaagcagcc
     1141 tctccattga tttctttcgg tatttttaag aacaagattt ttaccatgag cttgattaca
     1201 gccttgttga ttttttcttc caacttcttt gtcaatgtag tgattccttt ttatctgcaa
     1261 aatgctcgcg ggttgagtcc tagctatgct gggctgttga tgatggtctt tcctttgatg
     1321 atggtcgtcg gggcgccttt aagcggctac ctgaccgacc gggttggccc ggagattttg
     1381 gtattagtag gcttgggctt attgtcggta acccaggtca tgtatatgtt tttgtcagtg
     1441 gactctcctt tgtggctata cattgtcgct actgccatca tgggtgccgg caatgccttg
     1501 ttccagtccc ccaataatac gatggttatg agctcggtca ctatcgaaaa cttgggactg
     1561 gcaggctcga tgaattcctt tgcccgaaat ttggggatgg tcagcgggat ttcgctttct
     1621 acgacggtct tatatcaagc gatgtctgct aagatgggcc agcaagtaac taactatatc
     1681 gcaaatcggc cggatgtctt tttatatgga atgaagatta cctttttagg cagctttgtt
     1741 ttgtgttttg ttgccttttt cctgactatc tggcgtatca aaaaacaagg aggtattcag
     1801 aaaaatgtct gaaaaaacca ctgttgaaga atttttggag gcctgcgtat gggtgagtgg
     1861 gatttccatg gatgatggaa gatgaagtga acttctaaaa gccgaaaaac tcaagtatta
     1921 tcttcaaaat actagtgctc aatgagatgc ttgatttgat cacaagaaga ctgcgaaagc
     1981 cgctggcaac cgcaagctag gtatcccacc gatggactac gttttagagg ctactggtta
     2041 tgaatacgga gccaacgctc tcatctgcat aagaatacat cacccagatt atgtaaagat
     2101 tttcgatact tctgtcaaaa aatcattaag atagataaat gatttccgtc tagtcaaatt
     2161 ggttcagaat gtagacacac agatggtaag aagtcactca caattcgaga atggacttgt
     2221 cctatttgcc gtatacatca taatcgaaac gtcaacgata gtatcaatat tttgaccaag
     2281 ggctatgaat acaagcattg ccttagaaaa catcaagaac cttaggaaca acggggatag
     2341 cttggttaat aagagatacc gctgtttagg cttgcaagta tactctttta tcaagaattc
     2401 cgtggcttta gtcatgagag gcttaatact atgaaaattt tctttagcat ttttgattta
     2461 ggttcctttt ctaattataa taagatgacc actacagtaa cctactgaaa tatataaaca
     2521 gccactgtag taccattttc aaagtagtct atgagaggag aatcttcatt cttatgaacg
     2581 ctggaattgc ggatatgaat ttaataaaaa aaacccttaa tgattttact tccaattcaa
     2641 tatcaaaagg tactggtatc aatttaagca caataaaaaa gctgaaaagt ggtgaaagat
     2701 cagtggaaaa gctgaatctt ttagatgcta taaaaataac ggaatttgct atgaaaaatg
     2761 gaaaagctga gattgaaata tggagataat tatcgtctag tgaattactt gccacttagc
     2821 tgaatttaac gatccttggt gcttgaaggg ggagattctt gggaatagag catacttgca
     2881 agcctatgtc tttactaaca acgatatctc ttattaacca agctatcccc gtagttctaa
     2941 ggcttttact tttatctaag caataatcgt attcaaagga aatttttgac ccttagaaag
     3001 tctcatttta tcagacgtga gcttaggcat tgctgaattt tgttaattcg gttttaggtt
     3061 gtgctttgag cataatatga aagtattctt tatcacgatt tcattcatct aaggtaatgt
     3121 gatgtgattc tgaaattctt tcaaaggcag tttttgcata agctgacatt acatcatcaa
     3181 tgacatgttt acaatgtttc actactaaaa taagggggta accaagaaga aaaactgaat
     3241 gattatttgt atctaattcc atggtatatc aaccatttcc tatatagact gattatgaca
     3301 tggattaaaa taaaaaggca ctatgtctta attgtcggtt ttcgagttac cgaatggcat
     3361 tcatcaccca cctatagagg atgggcgact tctgcctgaa ataagttaca ctacttataa
     3421 cttctaataa aattagattg agtcattatt atttaacatc aacgattttc cagtttccat
     3481 ttttttgtaa tactaatttg tactgaaagt agctagtggt ttttgataca ttatcaaggt
     3541 attttatctt tgtactcact agaaaatttt ttccgttttg tagataaact ggattctcta
     3601 actccgaaaa ctttaagtca atattgattg gtcgcatcgc accattttca acataatact
     3661 ccaattcttt ttcagaagct gatggataaa gtttaaaaaa tgtttctaaa aaatcggtga
     3721 tttcttccgt tgttttggca tcaatttctg aatcgttttc tacttgtttt gcttcatatt
     3781 ttgccttgtc tggctttcca atcattgttg gcaaactagt tactaaatga ttcctgtttt
     3841 catcttcaaa gattgtcact cgatatgcag aagaaattga ttgcgtcttt tttccctctg
     3901 taatatgttg ctccactaaa aatgaaacga caaattcttc cgagtgtttt tcaacatcta
     3961 gaatttttac agattgaact tcggaactcg tgggaatatc tgctcgaacc atatcttgga
     4021 ttaaagcgag accgttttct gtaaggtact gttcgagatt actcattctc ttttcaattg
     4081 cttctttgtt atttttccat gaaaaatatt ctttcgcaaa atcctctgta aaactttcaa
     4141 cgccagaagt attcactacc ttctctttta taaccacttt ttcatgagtt gtatgttgat
     4201 caatggcggt gaaattttta taaacgccaa aagcaagact acaagacaat aaaagccaaa
     4261 gggcaagcac catttttcga tgcttaccca cggatacaac tcgttctttt ttcttttttg
     4321 ggctcttttc cttttgattc cgttcaattt ttatcttcat tctttttcct cttttctctt
     4381 atttgtatcg gccagctccg ataagatgct gccgccaata gttttccgtt agattcgtcc
     4441 accccactgg atctcctgca tgaaacatgc gattctcccc tgcgtaaatg cccacatggg
     4501 tcacataagt accggcatca taggttgaat gaaagaaaac taaatcacct ggtttggcat
     4561 cttttagatc aatatgagtc atggcatcat attgatcctg agccacccga ggaagtttca
     4621 ggtcaacttt ggcataacac cattgggtca agccactaca atcaaacccc gtttctggac
     4681 ttgagccacc aaacacatag gccgttcctt catacttaaa tgcctcatca aaaatttctt
     4741 ggactatttt gtcatcaaac tgagttgtag ttaggtattg cttcactaat aaacagtaaa
     4801 acatattgcc ataattatac cgccacccac cattttcctt aatcgcgatt gggttggggt
     4861 aatcgacttt ttgaccacct gatttttcct ttgaaaaaga ttcagctaaa gcaaaactat
     4921 gcttcttccc gtttttcccg acatgatcta gatacccgcc accatagtta taggcctgaa
     4981 taatactatc ttgatcacac cctagttctt tggaacgctt aaccaaagac gcaaaataga
     5041 gacagccttg tttgatggat gcctcggtac ttaatgaatt aggtggtaat ccggctgact
     5101 cagaactttg cataacgtct tccattgtgc caccagattc cacttgaata atcgccaaaa
     5161 gaattggaat atactctgtt atatccgatt ctttggcata cttttccacc attgcacggt
     5221 gtttcaatac ttcttctgag acactagttc cgccaggaga agtgccagaa ctaccatttc
     5281 cctcactgtc ttcaccaaac aacaaaccca cacacatcaa aatacccata aaagagatgg
     5341 tcagaaaagt taatgaaata acacccactt tcttcaagtt cattttttct cacctgacct
     5401 tgattttgga cgatggttca ttcttttttg tactttttga ttggtctgct ttggcagagt
     5461 ggacctctgt ttcacttgtt ggaaagaatt tttcttactt gctgattggc ttttcccttt
     5521 tgaaatgatt tcttgactag ttagttttcg tttgatttcg ggattcagat ttgctctcac
     5581 aatccgcttt ggtgcttctt ttttcagcat catatctttc tgaggttgag ctgctttatc
     5641 tattggaata ttcgcccttc tttttccctg acgatttgtt gcttcactca gtatcttgcg
     5701 tttttcgtcc attttcgcat ttcttcgttg tcgttgtttt tctcgcaaat ccactcgatc
     5761 cgctttttct tggaacagtc cgcgcttgaa ttcttctggt gcttttttcg acttttcgat
     5821 tcctttatgg agattgtatt tcaaattcgt tggcgtattc cttacctgat cctttacttg
     5881 tttagtctta tctttcacca aagcttgggt atccaaaact ttgccgattt ttctcccagc
     5941 caaattcatt cgggattgtt tcttattatt tgaaactgga ttctccttag ttaagtcaga
     6001 tagtatttcg tgatcgttcg gtaatcgtga attctttcgt agagaagaac cggatggctt
     6061 aaatttggat tttgattgtt cctttgccaa cttatttccg actgctgcac caccaccaaa
     6121 agccaaagct cgtcccacat ttcgattgag ttgccgcaat tttcgattca ttaacatttg
     6181 cggttttcgc atgacgcgtc ttcccattga ctggctatca ttactctgga ggttgaacat
     6241 actcataatg tcgcccaatt tgaaatagat accggcaaag gtcacaattt gcagaaaagc
     6301 aaccatgaaa aatggatagg ttgccgaaat attgaaaaac atcgtggaga tgctaaatgc
     6361 agtcgtaatc accaaagtaa cacccgctcg catcataatc gtattgaata aacgaataat
     6421 cgccttcttt cctaaacttt cataagtcgg caacatggac aataagaaac taattggcag
     6481 aaacatcgca aagataatga agagaatttg cgagaacaac ataatccctg ttagaagaaa
     6541 aacaaagaag gaaatgccaa tattgaacaa taccaaaaag accacggtcc ctaaacggtt
     6601 catggtcttg gtaatactga gattcgcatt gtcattgtct tcaatctctg ccttgaccgc
     6661 ttcttcccga tctttgcctt tgttttcatc tggactcaca gatagaatct tgttgacccg
     6721 gtcttcacca atttcttcta tgttggaatc atcaaattgc agtagtaacc aaggttgttg
     6781 gacttgaatc gaaaacaaac tatcccgaat caaatccaca ctatcttttc cttggctttc
     6841 tgaattgggt acaacgatct cggttccaag ggtaagggcc gcttcactaa catctgagct
     6901 aaagtcattg atttttgtga tataggtagg cgcataagca atgaacgaac ccgatagtag
     6961 aaaaatcatc acaaaattaa tcacggctcg aactgctttc gtggtttctc gttttaataa
     7021 tccggtatac gccacataca cccccagagc taaaatcatc aataatagaa acccaaaata
     7081 aaatccggag gtctggagtc cattttcagt aataccagcc aatgtttgga tattctttcc
     7141 aatactttca gccgtatccg aaatgaaatc taatttgtaa gcctcttgaa ccacataacc
     7201 cgtggcgttc gacaaataca aactcaccgt ccaaatgaaa ttagtaatgg cataaagacc
     7261 atacatcacg cttttcccta agccatcacc ccaattccaa ggcaaccaat cccaagagct
     7321 atccacaaag aaatctagtt gatagttgtt cagtgaatat ttggaatata gatttcctgc
     7381 ttcaactgta tcatcaacta gccctgtcgc ctcggctacc gtaccaacta aactcaatag
     7441 aactatcaga gtgagtatgc taataaaaac gattcccaca atccgaaaaa gtttcttttt
     7501 catcccctca ctccattctc tcttgaacag gtggacgggt atcaaaggca tggaagagtt
     7561 cttcaaagac tggatgtacc tgaaccacgc ctacccgacc atacaaatcc tgcatcaagc
     7621 attggccatt ttccaaatct cgtaggcgtt tttgattgct ttcatcttct cgatccaagc
     7681 caaagaattc caaggttttc ttgatttcat gaatatcggt actgcgaaac gcaaacttca
     7741 acccgatatt attcttcaag cgttcatcca gtaaatcatc actattttgc gtgacaaagt
     7801 aaaccccggc attcatagcc cgcccagcac gaacgagctt attcgataat gttttccctt
     7861 gcgctacttg aagaaaactc cacgcctcat ctaagtctac aattttgaag atttctcgat
     7921 tactatggat aaagtccaag gcaaaagtgg aaatgacgat tagcatggaa acactaagca
     7981 attccatggt ggtatattct tcaaaagtgg tttctgcatc tggtaaaacc aaatccgcta
     8041 cttgaatgat atttaactgc ctatctaaac taatcgactg ggtaatcgaa ccatctgaga
     8101 aaagcaaatg ggcaaaatca taatcggtaa aggactcgat gtgatccgcg atattttccg
     8161 caactggtga gccttctttt cgaagctcat taattacttg taataatccc cgttgattgc
     8221 tttgggtaac cgtccgaatg gccttacgca acactggaaa cttctcccca tctcgactag
     8281 aaattcctgt taaaaaggtc agaatatcaa ttgctagact ttcagaatcc ttctttttct
     8341 ccataatcac ataaggatca agtaatcctt gattttttgg cttactagtt aagttgagaa
     8401 tattgatttc atccgcaatt tctggcaagg tttctttcca gccaccccgt tccccttttg
     8461 gatcgacaat cacagcttgt ccaccaaaaa ggacggcgta ataaactaag agattgttac
     8521 taaaggactt tccgccaccg agtgaaccga ggaatgccgc tgctagcgca ttggtaactg
     8581 aatccttgac cccttgtgag gccaatgccg gtttcaggta aacattgcgc cccgtatcta
     8641 cgttataacc aaagtaaatg ccttccaact cccctaacat ctgagtggcg ccaaaaccaa
     8701 gtccggcgag aaaatcagac gtaacatact ggatataatc attcatgtat cgcttactag
     8761 aaggtaagaa ttcttcatgt agccccatca tatcgccaaa aggccggact agttttacat
     8821 tggtatcatc ataaaaatct agcacttcat cacaccggcg ctttagttca tctactgatt
     8881 cggcacttac tcgaaccaca tagctcagtt tgtacatgga ttctttggac tgatccaagg
     8941 tagtttccaa ttcatctacg gaatccaatg catctaagac attggaattt gtttcattgt
     9001 cagattgata ggcatggttg tccaagtctt ttaattcctt tttcttattg cgaacggtag
     9061 caagtgcctt tttgttcgtc acaatctcca cattcatact agtgtcaatt gggaatgtaa
     9121 attgttgttg ctggtaatag aaaagttctg aggagggaaa ctccatttcc cccacaattg
     9181 tattaattgt taagtacgct acatatgatt catgattctc atgttccatg cataagtacc
     9241 ggggcttttc ttcaatcaaa caacgactgg gacgtaataa gtcgtaacgt ttcaccaaag
     9301 tttccgactt cagctttttc tttggtagct gaaattcata ttcttcaaat ggtgtaccct
     9361 tttcgccata gatatgttca atcaaataca tcagatcact cggtgtgact cgtctgactt
     9421 taaatcttcg agccaattta ctttccatta gtttttcaag cttggtatat cggcgaattt
     9481 cttcattgga taaggaaaca aaatcgccca tcaaatgatg attcactcca tagacaaatt
     9541 cttgaaagcc ggagaagaaa gatttcttga gattctttag attgacctct tcatctgtcg
     9601 caatcagttt gaagccaata aaaaagcgat aatcaatctg actatcgcca atcatggata
     9661 ctaatgcctc tgtctgtaaa tcaattcgtt gtttcgctac ttctgttaat cgcccggtaa
     9721 tttccttttt tgatcgttct tgcgtggcac gaatactact ttctgtggca atttgtaagg
     9781 catgaatctt gccttcacga ttttgcgcaa tcaattgacg aaagttatcg tggacttgat
     9841 atttctgttc aggtgacaaa aaggagtaat tgtaaggtat taactcatag taggcaaaac
     9901 attctccctc ttgattaaag acaagattat tttcaatata cttaatcggg tacatagttc
     9961 acactcctta ccagagtaat gggttcgtcc catcgtttct tctgttcttt gactttcttt
    10021 cctgcgaaag tgaccttaaa tcgtaagtga tagctaataa aagatcgtaa aaatcccaat
    10081 ggtttcttac cgtcaaacgt tttttgcgac atgaaccaag taactgccac cggtatacct
    10141 aagtatttta aaaaggcccc ctctatcata gatagtggag gcacatgggc aaatacgatg
    10201 acaaacagca aggataaaac aaaccaagcc atctggttaa aagtcacagg aaaaggcagt
    10261 tgaaagtcat taattgcata aatgaccttt tcaactgccc aaatactggt gtagcttttt
    10321 attttcttca tcaaatcatt cctttaaaaa gaaggatagt ttagaaatca actacccttc
    10381 aataattttg ttaatatgga tactcaaaaa cacctcgact cgttaccaaa taattgcctc
    10441 caatctccaa gtctctacca cacgcatcaa aatcaatata cgtcttcaag tgttctggta
    10501 cttctccaaa atcaccagct tctacaatca agaaatatgc tacatcactc atatcatcgc
    10561 aatctggata atagataata tcatccacat gctctaccat ctcctcaaaa gaattgaaga
    10621 aagcatgttg aatctcagaa gcaacttcac caactggcgt tccttcaagt tcctccgcta
    10681 agccacacaa ccgattaatt tcctcaattg gcgtatactc atcaatctca aagggaagtt
    10741 cataatcatg aattgcatat tcctcgtatt cgtcatttaa cccaattcgt tctttcactt
    10801 catcaaaatc gacaggtggg gtaaaccacg cccccaccaa ttcaccttcg ttatatttcc
    10861 cgaggttcgc aatataaaca cgcatctgtt ccatcgtgct tcccccttac gcaccgacaa
    10921 ttcggttaaa caaactgagt aagacatcct tcaccccaga agcattaaag accaatccga
    10981 ccgcaatcaa agcaatcacc aaaaagccaa tcagtttgga aaactcccgc ttaaatccta
    11041 aaaacaaacc aatgaccaca attgccagta agactaagga ttgggcattg gttaaaaacc
    11101 attgatatat ggataggtaa gttcctgata ttataaatat ctcaaacttt tccccccatt
    11161 cacacggagc gtgcgacttt caccgcactc cgcgttccat cgtgtttata ttgaaaagta
    11221 tttatgtagt agacctatat caagtttaat tcttgattgc cagctaatac tctatattta
    11281 tttagtctta tgagctggac tttatttagc ttcaatagag ctatatattt ttgtattgtt
    11341 tcttctgttt tagcgtgtat tagacggtgt accgcctctt ttaccaaaat aagattttta
    11401 taatcgtcat ttccaccagc tccagttggt acaatatgat gacaatgtat ttcattagct
    11461 tgttgaaatt cttccagagt tatagcacat tttccatgtt gagcagaaaa tagcgaaatt
    11521 cgattgtctg cgtattctat tgtgcgattt tccaaagata atttcattaa ctggtacatc
    11581 agtaatttat caacacttag attggtgtgg ataaattttc ttccctctag agtgtagcaa
    11641 ttcgctaaat acacctttgc cattggtttt ctgtgttgga tatatccaac agggaaaata
    11701 ggttcgccct cgccagcaac ataccgaagc attttagatt taccatatct tgttgcctcc
    11761 tttgtcgtaa gttgtctgcc tgtttttctc aatctgcccc ttttgtttgt acctaatctg
    11821 ttagtgaaga tagccattac agctctattc agtttcatac aatccagatt tacattcgtt
    11881 gcaatctgat aataattttg catacctaat accattgaat tataaagtct tgtttcttct
    11941 aattccgttt tcccttttga gggagaagca attttctttg cctgtgatac cagcttgtct
    12001 ttttggtttt tgaattgttt atcacttata tgtgatttaa caactaactt ctttcttctc
    12061 ggtatcattt taatcttgaa accaagaaag tcggagtatc tttttctaac atttacaatt
    12121 cttgtttttt cttgtgatac ttccaatttt aatcgttgtt caatccattg agtgattgct
    12181 attttggctt tttcagcact ttccttgtag cggcagaaaa ctctaaaatc atctgcataa
    12241 cgaaccatat acatttcctt taactttgtt ttcttcattt ctttgtaagc gttgctttta
    12301 catgggctac cgcttttatt gatatgaacc acatagtttt ttgtaacagg attttcctgc
    12361 cattggcttt ctacccaatg gtctaactca ttaagaacaa tgtttgctag tagtggggat
    12421 attattcccc cttgcggagt tcctttctct ggatatgtta ttgtaccgtt agtcattttg
    12481 attggagctt ttaatatcct ttttaaaatg aatatcaagt gcttgtcccg tattcccata
    12541 gcccatatct gttttatcag cttggaatgg ttcacgttgt caaagaaacc cttaatatca
    12601 aattcaatca cataatgtag ctggctgatt tgtaggcggt tataggttgc ttttattgca
    12661 ttttcaactg acctattagg tctaaaacca tagctgtttt cactaaattt agcttcgcag
    12721 attggttcta atacttgttt tatacattgt tgtataagcc tgtcccacat acaaggaata
    12781 cctagaggtc tggttttacc atttggtttt ggtatttctt ttcgcctcac tggcttaggt
    12841 cgatacccat gtggactgcc tgcaacaatg tatcttactt ttttgacaac tgtttctgct
    12901 ggcaatttgc caatatcttt aattattgta ccgtctgttc ctgctgtttt actgcctgca
    12961 tttgctttga tatttcgata tgccagtaat atattttctc tcgataggat aatatccata
    13021 agatgagtga aaatatctcc attcttactt ttggcatata aatcatcaaa ggtgttttgc
    13081 atttcgtaat attcagcgtg tcttaaatct tctacacaca atgtttcatt tttattttta
    13141 ggcataaggc ttcactcctt tacttgaaat tgagtgagct tttttatctt actcgtaatc
    13201 cttattgaaa caacataata cttttctaat atatacacga cttgagggca ttcctccatt
    13261 ctcattacaa gaatttctta ggttcgccct ctgcttttca gcattagttc aactgcttat
    13321 tgttcttcgt cagtttattc tcttgatgag aacccaatac ctttcctcgt tccgataatc
    13381 ttatctatac atatatatcc ttaggtcact agctataagc ctgctggctt gataccgcct
    13441 gtaacgttat atggcatttc ataacctata ttcttacttt gccacaccag ctacgatttc
    13501 tcgtaccttt gcatttctac aaagttcaaa tttagacccg tacattcgct gtttcgtcag
    13561 ttccttatag aacattctaa ccatagatat tttatagacc cccgacctgt tggatacgct
    13621 acccacctct ggatagtttt cgatagaaga tatatgttca tcaactatta cggtatcttt
    13681 cagccgtctt taccgagctt caaacattct gagtttcctc atacaatgct tgtcggaggc
    13741 tcagggcagg cgtttcagag cgttacttca tcattccacc tgtcagatta tcagttctcc
    13801 taataaccaa actttttcgt tcttttatta gtgccttagc ttttcactaa ggaacgagtc
    13861 gcacataaat tttgtccgaa attcatcttg tcactttcct ttcttttcca ttgctcgatc
    13921 cgctgctgca cttgcattca tcaagcacat cacagtaaca ccaagcatac tgccaccaaa
    13981 taccaaaaaa atatctttta caattcccat gacgattcct cttttcatat aatcaaattt
    14041 tcaattgcca tactttgttg ctccaaaatc tttttatgtc ggtctgataa gcgtgcattc
    14101 tgtaccatat cctggatata ggttgtccga ttcaatcgat ccaactcctt agccattttc
    14161 cacgtagggg cgacttgtcg gcctagccaa ttcaaggttc gttcaatggt atacggtttt
    14221 ggctcggaag ttaattgaat ctctttccga ttccgtccaa taaaacgccc ccattgttca
    14281 ttcaagggcc agttggttcg ccttttacct tctactttat ccgcaaatcg caagtaacga
    14341 ttgataatgg aaaacgtggt gctctcaatg ctacgacctt ttaacaaatc ttgaatcgca
    14401 tgaaaagcac ggtcattttt cagtcgaatc tcaaaacgat ttttcgtctc agtttggtct
    14461 aatggaatcc ctaacttgat atattgttca tagtcttttt catacaaaca gaaataaacc
    14521 tcactcttga gactgccgat atataaggta ttgcccatgc cgtccttttc atcagccttt
    14581 aaaagttccc cggaacgata acttttaaag gtacgaaaca aggagataca ttcttccttc
    14641 tgacattttt tcgttagctc aggtatatcc aatagcccca ctcgatcatt gattgccaag
    14701 tccaagcgtt tcatcacgcc accggccttt agacaatcct cgaaaaagtc gtaccagcta
    14761 cgcttttgag cgagtaaaaa agtttcaaat tgtcggcaac ctcgcccctt taattctaaa
    14821 agcacacctt tatcttcttc atgagacaac atcaccacaa tatcacccat gacatactgt
    14881 tcttgatagg agtaaaaggc ataatcttca tggagcatat actcaaattt caaatggaga
    14941 atttcttgaa taatttttcg tgcatccgtt gtgggaaagc gaatccttac atagtcaaac
    15001 agtaacgtca aaggcaaatc gggattaaag ctttccagtt ggtcaaaaat tttcctcatg
    15061 gtttttatag atgcggttac gccaccattt tctatctgat ttaagtacgg ccgactaagt
    15121 ccacaagccg tagccaattt attttgtgaa acgccgtatt cgttgcgtcg gtcttttaat
    15181 agccgataat ctaaattccg ttgtgccaag ttctcacctc caaattcaag gtgttaactt
    15241 tttcaaatga gttaacacct atacaaatta cttactatct ttgatacaac aagcattctt
    15301 aatggttgct tacttatttt ttgtgttttt gtacccccct gttagatacg ggggtgagac
    15361 atcatggtcg gctaacgccg tccatgaaca ggcacagcac cacggaggat ttctgcttcg
    15421 cgaatcctcc gcggtcagcg cctaacagtc agttgtgttg gtttaggtta ctcatctgta
    15481 ataccattta cttccaactc tgctttcatt tctaacaagt attgacgaat atctggttct
    15541 tttgcttggt ccgcaatccg ctcaaattct gcaattactt gatttatttc aattcgagca
    15601 tccgtctcct cattcattaa atatggatcg ccagcgactt tgacaccatt cttgcgaata
    15661 tcaaatttta accgtcgcaa ttgttcttct aattctgggt ctgactgaat ctttccaatc
    15721 acccaataaa tttctccaat caactcatca taaaccatgt acatccaact cttttaatct
    15781 tctggcaata tcaaaaatga ccggaactgt tacagagttt ccggcttgct tatacaactg
    15841 actatctgaa ttgacttgag aatctcgatc aaaagcccaa tcgggaaagc cctgaagtcg
    15901 ccaacactct ctaggtgtta gcttccgaat ttgatactga tccgtgacga tcgcttgttg
    15961 gtctcctgtt agtaaggtat gaacactctg ttttcccaga cgacctcggc gacttgtgga
    16021 attaggaaaa gccagattca cactgtcttc tggcttcacc tcttgatatt ctgtttcatt
    16081 ttttcgaatc atgatttttg taccaccttg tttcgttgac aaggtggggg cggttccagt
    16141 aacttcataa actcgattac cttgttcaaa ctttcccggt aatagtcctg ctacagagat
    16201 tccatccctt gctggcagtg cgattttgat tccttcatca cctgttaagg ttggcgctac
    16261 gccatctgaa ccatagactt gtccacccat tcctcgattg gaaggattga tatttaccaa
    16321 atttctgatg ttttgatcag atgttgaaga cggtccaatg ataggaaata cttgttcggt
    16381 acatgcgtct cgagaatgtc cgataaggaa tatccgctcc cggctttggg gaatgtaggc
    16441 ctttgagtta agcacctacc actccacgtc ataccccaat ccatccagcg ttcggaggat
    16501 cgtctcgaac gtagcccctc cttcgtgatt gagcaatcct cggacgttct caaggaataa
    16561 aagcttaggt cggagaagag tggtgaaccg agcgatttca aagaataaag tacctcgagt
    16621 atcggaaaat ctttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccgccgca
    16681 aaggatgtcc actggtccga gggattgaat aaactcatct gatacacttg tgatgtcatg
    16741 catttccacc tccttacttg tatcatgaat tgctttataa ctacgtctag caaatttatc
    16801 aatttcacaa aaaccgatac aatgatggct cgcctgttcc atacctaaac gaaagccacc
    16861 aataccggcg aataaatcta aaaatctcat acattacctc attcattgca aagaaaaaag
    16921 ccaagaacat tttgctcttg gctcatacat tattaatttg tcctactatt ttttatttaa
    16981 tccaactaat ttccgttgtt tttcattggc ctgatcggct acatatttaa tcaccgtttt
    17041 gaactctttt gcagtagcat tttccgataa atccgcaatg ctcttattgt atttctttac
    17101 aattttcgtt acttgttctt tctcagtcat gtaattgaac ctccaaatct ttcctactat
    17161 accctagtgg taaaaataat tctagattat ccgtagcata atgaacttcg gaaatattat
    17221 tcgtataatc ttttattatg atatgaagca atacatctgg atgtttactc tttacttttg
    17281 caatgttctc tcttgatttg aaaaaagcgt taataaatgc ctttttcggc acatgacgtc
    17341 cctctgcaat ttcacgttct tgtgtaaatt tccaagcgat tttagggtct tgataaacat
    17401 agaaaatcgt cactcgaaaa tgttttttca acgcccgcga gatatttttt tcagcactta
    17461 atattgcaaa agttgcatcc agaataaaag aatagccatg atctaagaca tatctaaatg
    17521 cctgctctac caaccaagaa gatgcttttt gaaagagact agagtttttt ccattataat
    17581 caggaaactg ggtgcgaaag taatccgcat caataatcac atggttatca taattctcag
    17641 ctaatccaat ggcaacctct gattttccag ccccaggact ccctgccata aaaactgcgt
    17701 ctcgtccaac ttcgggggat ttccctttga taatataaga aataaactta tccttatttg
    17761 cttttgcata ctctaaataa gaatcattac tttccatatt aattatcccc tttcttccta
    17821 ctatataaaa gtattatacg ttacttgaag tataaagtca gtattgaaga tcactcaatc
    17881 attcatcttt tctaatttac taattgaatc taagaaatca tacccttttg gtacaagtgg
    17941 cgtataaaac tcactaatca cactgccacc tgtatccaca taacctcgac ctttgatgtg
    18001 cttcataaag aagttttttt cgacctcccc aaacatcatg gaatatccga gttcactcat
    18061 acgacccaaa gccacgcgaa agttgaattg atcgcggatt ccgtctccta aatatttcgc
    18121 atccggcctt tggcaagcca aaatcagaaa gaaaccagat tgacgaccca acatcacgat
    18181 ttgtttgagc ttattcaaaa tcaccgcact ttctttcgtc gttaacatct ccatataagc
    18241 cacgtattca tcaaagatta aaaagttggg tggaagtcca agataggcat aattctctcc
    18301 tgttttgtag ttagacattt ctttcattgc cttgctacga gccatcatac gttcatagaa
    18361 atcttccaca caagcagaaa tttcttcctt ttgtgaataa acatgaggca tcaccgtacc
    18421 taaatcagct aaatctgcat ttttgggatc gaggataaac aattctgcat ctgacttcaa
    18481 tagtgcttcg ataatggtga ggaggaaata ggtcttccct ccacccgtgc caccagcgat
    18541 taacatatgg ggtaaagaat cataagccca tacttgattt ttcatcaatc gcagagtccc
    18601 attttctgcc actacttcgt ctatcccaat tcgattggcg atcatatcat acagtaaagt
    18661 atactccaca taggagtctt tgagttcctt ttctactaac tcacaataca gtccgctttc
    18721 taacttcttt tccaatttta aaagctgatc ttggtatttc ccaagtgaaa tttgaacgcg
    18781 aatagacagt aatccatctt tcattcgata acagattttg gggaagtaac tgatagtctc
    18841 tctggttcgg ctactattca aatctttgaa aaagccttca ctttgagttt gtttcacttc
    18901 ataccaatgg ttctctagaa tcatccgagc tagcttttgt cgatgccaca agcttcgcca
    18961 atgatcaaga aaaaagtgaa tataaagcaa tatcatagct cctgaaaaag cgagacttcc
    19021 aagcagacat actaaatctt ttatagacca aacgatttca atagtagaaa gtgatagctt
    19081 agaaatatca aattgtctaa actcttgaaa ataaaagacg ctcatccaac cgatgaacag
    19141 agcgagtagc cagccaagaa caaagtgata aaccaagtgc tgatctcctg cgcgtatgcg
    19201 atgtcctttg tacattttga tggtttccac ctccaatcga aaaagcccaa taggatttca
    19261 ttaggcaatt tcaaaaagtt atgctagtca tttactgcta cgaggagggg agttgtttcg
    19321 tttcaaccaa ttgatttaaa aagccttcaa tagcatccgg catctccaca ccaatttctt
    19381 ccaaaatgga ttctgcctgc cgacataagt tttcatttac ttcgacaact ataaagaaag
    19441 actcttcttc tcctaaccag tctagtacat cagagtcttc ttcactgtcg ctattttctc
    19501 caagtaaacc ttgatgtata atcagaaaca ttttttcatc agaggttaat gctttaacga
    19561 gttgttcctc agtcagctcg ccttttccat attgatcgag acaatctaaa atatgttcaa
    19621 cattaatttc cataatcttc acctatttct ttctttcatt ttggttcgta ggatttcctt
    19681 gattcggctg ccgcttcaag actaaatcat ccgccttaat gaaccaatta acggtagttc
    19741 cttctcgaaa aacataattg gcaacggtat ccacgattgg attgaccaac tctacctctg
    19801 catcataagc aaactcgcgt aagggtacat tagctgggat actaacttga atcattcgcc
    19861 cttgtgcaga ggattttaaa ttataggttc gttcttttac ctcattacca attgttccat
    19921 cctcattttg cttccgtact tctcggcgta atgcggaaaa acgcaacttc ccaaatgtta
    19981 aacctgaatc tacgacaata ccttctgtta atctcatctt cttttcctcc tatgctttca
    20041 ctaaatcatc aacatacaac acgtaatcag taaacccacg attctcaatc tcatagcctt
    20101 tggcaaccaa atggggattc acggctttta gcggttggtc ttgatcaaaa tccttttcac
    20161 cggcttccgc agctactacc acctcaatat catcggcccg ttgcttactg gaatacaaat
    20221 gataactgcg accaatcacc gtagtattcc gtccatatcc ttctgttata atttctcctt
    20281 caccggcata acttaacttt ccaaaggttt cagccatatc tggcacgaca aatttcaatt
    20341 ccatcttctt ttcctctttt cattgttagt tttggataca aaaatagcgc gactactttt
    20401 ttaggaggcg gtcgcgcacg ttggttctta ggatttactt gtatcatctc atcaccttag
    20461 ttccggcgat tccagaaata atagcccgcc gtcgcaaaaa tcaaagtaaa accaatgaac
    20521 agtaaaacat tggaattttt ctccccggtt tgagggaagg tcttcactgt gggactagat
    20581 tcttgagagc cacttggaac agttggtgtt tccggttgtt tcggcgtact tagtatggtt
    20641 ggaacttctt ttggtgttaa ggtttgagat ttttctttca aatcttcatt gtgttttcca
    20701 ttgatattcc catctttatc gtaattgatt tccgcaaaag taaacttggt tccttctgga
    20761 tattttccgg tatctacttt tttcgcaagc acagttttgg tgaattcttt atcatttact
    20821 tcataatcaa tcttgccaga tttccaaatt tctttgtttg taccatccgg taataaagca
    20881 tacagaattg tttcgaaagc ttcttttgag ccatccagta catcatgagt aatcgacaca
    20941 tcatcaaaca tatccaccac atcaccatga gtaaaggtct gagaaccgtt ttctaggtgg
    21001 gcctttgttt ggatagaaac atggtgttcc actgtacaac tgacagtttg ggcttggtta
    21061 ttcaagctaa catccttcgc tactggctta tcgcctgctt caaaagcttc cttattttca
    21121 tacacatagt tgaataagac aatagtttta tttaaaactt gttcggcggt taatttatgc
    21181 tgaattttcc aagttcctgt tttctctttg ttcgccaacg ttgcgcttgt ttctgcaatc
    21241 accactggtt tggctttttc atctttttct tgggcagctt ttgtcgcatc cacatcaatg
    21301 gcttgcgcta ccacatacca gtcttctttg atttcatgta gattgtaact caatttatca
    21361 actagctcgg tagaatccag agaagttaat tctttgtttc cgtctttcca agtcgcaaga
    21421 gaagttaaac tatcttcttc ctcaggcaaa tcatagagca ttaaacggtt caaactaaca
    21481 gaaaatgctt tgttcgtcag cttctcgtaa ggaacggtca ccgttttaat tggttgtttt
    21541 tggtcttgtt cggtaatggt aaagacatac tcactcttca caaagtcttc tttgttttcc
    21601 ttaaaagtag aacggatttc taatggcgta atcttttgaa aaccttctgg ggcttctact
    21661 tcttcaagta aataatcccc ataaggaaga ttttcaaact tgccataacc atcaaaccct
    21721 aattgctcgt tataagctgt ggtcgcttca tcttcggcac ctgtgatttc attggtccct
    21781 tccaatggcg acactttaaa ggtcagatca ttgaagccag tttcggtagt gcccgcagct
    21841 gatccagcaa atttaaagaa atcaaagcca aagcgaatca cttgttcttt tgccgtaaca
    21901 tttcgggtaa tcacggcatt tttttcgcta tcatccacct ttttgatgga tacaggatac
    21961 tttgtttcat caaggctata tccttcgggc gcttttgttt cttgccaata atactcgttg
    22021 atagctaaat gtttaacggc aacttggttc ttttcgtcta aagtcaaagt gaccgtttca
    22081 tcagaacctt tcgttccctt cgctaactct ggtttaaaag cctcactcca tttaacagct
    22141 tttccatcct tagcagtaaa caaggtgtat tcagcaccct caaagactgc cttaccttgt
    22201 gctttatccc cagtatcttt gtcttctttc gtcaaagtgg tttcgccagt gacttcttga
    22261 ttttgccctt ttacgttact ggtaacaggg gctacggttt gattggcata cttcaattcg
    22321 acttttacag gtttgaacgt attcacgaac ccatgactag ctttagtttc ggtcacataa
    22381 taggttccca attccaacgc attagcaatc tcttttggtg tttccgcatg accgttttca
    22441 tccgtggtca tttcttgaac gatttcaccg gttggactgt ctttgcgaat ggcaaacgta
    22501 ttaccagcta aggaataatt gtcattccaa agatcactcc ctgtttcaac tccgctttta
    22561 tccagaatga tttgcccttt ttcccgtgcg ttttttgaag tcacgtaaat ggtttcgcct
    22621 gctttaatgg tggtagtcat gggggtggta tcaatcgtat aaggcgccgg tactgatttt
    22681 tcggtaatgg ttacttttgt accatgagga atctcatcca atgtagcaat gccttctttg
    22741 tcagtagtca cgtcttttgc aggtaaagtt tttccaaagt ctaaatggaa aaccgttcct
    22801 ggtacaacat cacccgattc tttatcgact tttttgatct ttaaagaacc ctccgtttcc
    22861 acatcaattt tgattgtgta ggtattaggt ttatcaatcg ccccggccat taaagtctgt
    22921 tggccagcct ttttataggc gacaggtgtt ccggtaccag cagatttctt aagcgtcagc
    22981 acaccacttt cttttgaatt ggcatttggg gtgattatca actgattacc attcacacga
    23041 taatcaatgt ttgcggcatt ttccacaacc tcatcgaact ctgacaaatt cagattattc
    23101 gtatccgtca cagtagtcga ttgccctaat actgttttag ccgtactatt atggaaggtt
    23161 ggtttcttct gataatcgtc aatggcttga tttatttttg cctcaatggc tgcgatattt
    23221 acggcactac catctgatcg ttttattgag tgaagggtat acccattaac ttcttgccag
    23281 atcatcttct gtgccacaat atgagtatct gcatctgtac ctactttttt ccataaaacg
    23341 gaaactaatt tggctttatc ggacatatct ggtaacgggt ttttctcata gccaggagtg
    23401 aactcattgt aaatcgggac tcctggttca atacagaaaa tatcttgttt aaagccgttg
    23461 tacactgcat aatgcggaac agtgacttct gacattggac gaccatcact aaaagttcct
    23521 tcaacagcat attgatgtgc taaatctagt tctacggtta ccgtttgtgg atgggtaatt
    23581 tcatcagcaa aagtaatcgt aggagcaatt gctccaacaa ttgcttgaac agccaaactc
    23641 aaaatagtta atacacataa tttctttttt tgttttttat acatgaaatg ttctcctttc
    23701 aaattaagca caaaaaaata agatgctcgc ttgaacatct ttgtttataa agtcttttta
    23761 aaatttttat tgactgaata
//
LOCUS       DAZKMB010000033        23642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000033 DAZKMB010000000
VERSION     DAZKMB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.033"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG8V3_001170"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG8V3_001170"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0299720.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="ACG8V3_001171"
     CDS             complement(1171..1866)
                     /locus_tag="ACG8V3_001171"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0299721.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="ACG8V3_001172"
     CDS             complement(1879..2595)
                     /locus_tag="ACG8V3_001172"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0299722.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="ACG8V3_001173"
     CDS             2925..3533
                     /locus_tag="ACG8V3_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0299723.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="ACG8V3_001174"
     CDS             3858..4181
                     /locus_tag="ACG8V3_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0299724.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="ACG8V3_001175"
     CDS             4363..5733
                     /locus_tag="ACG8V3_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0299725.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="ACG8V3_001176"
     CDS             complement(5863..7164)
                     /locus_tag="ACG8V3_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0299726.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7518..9509)
                     /locus_tag="ACG8V3_001177"
     CDS             complement(7518..9509)
                     /locus_tag="ACG8V3_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0299727.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9653..9919)
                     /locus_tag="ACG8V3_001178"
     CDS             complement(9653..9919)
                     /locus_tag="ACG8V3_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0299728.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG8V3_001179"
     CDS             complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG8V3_001179"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0299729.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG8V3_001180"
     CDS             complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG8V3_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0299730.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13263..13826
                     /gene="def"
                     /locus_tag="ACG8V3_001181"
     CDS             13263..13826
                     /gene="def"
                     /locus_tag="ACG8V3_001181"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0299731.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14079..14600)
                     /locus_tag="ACG8V3_001182"
     CDS             complement(14079..14600)
                     /locus_tag="ACG8V3_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0299732.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14603..15445)
                     /locus_tag="ACG8V3_001183"
     CDS             complement(14603..15445)
                     /locus_tag="ACG8V3_001183"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0299733.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15438..16163)
                     /locus_tag="ACG8V3_001184"
     CDS             complement(15438..16163)
                     /locus_tag="ACG8V3_001184"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0299734.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16339..16635
                     /locus_tag="ACG8V3_001185"
     CDS             16339..16635
                     /locus_tag="ACG8V3_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299735.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16891..17065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17203..18795
                     /locus_tag="ACG8V3_001186"
     CDS             17203..18795
                     /locus_tag="ACG8V3_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0299736.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18808..19548
                     /locus_tag="ACG8V3_001187"
     CDS             18808..19548
                     /locus_tag="ACG8V3_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299737.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19716..21065
                     /locus_tag="ACG8V3_001188"
     CDS             19716..21065
                     /locus_tag="ACG8V3_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0299738.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21193..21783)
                     /locus_tag="ACG8V3_001189"
     CDS             complement(21193..21783)
                     /locus_tag="ACG8V3_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0299739.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG8V3_001190"
     CDS             21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG8V3_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0299740.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6770 a         4761 c         4502 g         7609 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattctg tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 cttacccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat aatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aaaacggtat tcaaaagctt gcatctacgg cattaagccc
     7381 aaacagatga aaaacatgag aagctgtttt tatctgctcg ttcttactgc ttgttgcagt
     7441 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
     7501 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
     7561 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
     7621 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
     7681 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
     7741 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
     7801 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
     7861 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
     7921 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
     7981 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
     8041 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
     8101 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
     8161 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
     8221 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
     8281 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
     8341 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
     8401 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
     8461 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
     8521 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
     8581 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
     8641 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
     8701 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
     8761 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
     8821 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
     8881 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
     8941 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
     9001 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
     9061 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
     9121 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
     9181 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
     9241 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
     9301 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
     9361 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
     9421 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agtcattgat
     9481 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
     9541 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
     9601 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
     9661 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
     9721 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
     9781 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
     9841 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
     9901 accataaaaa attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
     9961 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    10021 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    10081 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    10141 attttgccgt tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga
    10201 ggttgtaaaa aagctgttct gcatcaagta ataagaacag ccaaataaaa acagctccta
    10261 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    10321 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
    10381 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
    10441 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
    10501 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
    10561 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
    10621 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
    10681 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
    10741 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
    10801 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
    10861 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
    10921 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
    10981 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
    11041 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
    11101 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
    11161 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
    11221 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
    11281 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
    11341 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
    11401 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
    11461 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
    11521 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
    11581 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
    11641 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
    11701 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
    11761 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
    11821 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
    11881 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
    11941 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
    12001 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
    12061 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
    12121 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
    12181 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
    12241 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
    12301 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
    12361 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
    12421 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
    12481 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
    12541 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
    12601 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
    12661 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
    12721 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
    12781 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
    12841 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
    12901 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
    12961 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
    13021 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
    13081 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
    13141 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
    13201 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
    13261 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
    13321 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
    13381 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
    13441 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
    13501 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
    13561 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
    13621 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
    13681 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
    13741 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
    13801 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
    13861 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
    13921 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
    13981 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
    14041 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
    14101 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
    14161 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
    14221 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
    14281 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
    14341 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
    14401 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
    14461 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
    14521 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
    14581 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
    14641 gtttcatcaa atcaacatac gtatctgtta ttgtttcctt atatccctta tttttcagca
    14701 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
    14761 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
    14821 tcaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
    14881 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
    14941 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
    15001 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
    15061 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
    15121 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
    15181 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
    15241 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
    15301 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
    15361 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
    15421 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
    15481 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
    15541 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
    15601 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
    15661 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
    15721 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
    15781 tttcgacaat gacattgata caaacttcca tgaaaattga cgagttgcgg gcttttagcc
    15841 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
    15901 tgtgccattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
    15961 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
    16021 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
    16081 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
    16141 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
    16201 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
    16261 acaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
    16321 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
    16381 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
    16441 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
    16501 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
    16561 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
    16621 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
    16681 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
    16741 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
    16801 ttttcaaaaa acagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
    16861 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    16921 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    16981 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    17041 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
    17101 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
    17161 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
    17221 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
    17281 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
    17341 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
    17401 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
    17461 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
    17521 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
    17581 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
    17641 gcaaaaagtt tagcagatct ttctggagca aagattactg ctcagttaaa tacattccac
    17701 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
    17761 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
    17821 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
    17881 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
    17941 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
    18001 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
    18061 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
    18121 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
    18181 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
    18241 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
    18301 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
    18361 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
    18421 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
    18481 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
    18541 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
    18601 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
    18661 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
    18721 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
    18781 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
    18841 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
    18901 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
    18961 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
    19021 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
    19081 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
    19141 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
    19201 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
    19261 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
    19321 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
    19381 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
    19441 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
    19501 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
    19561 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
    19621 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctt ttaatatgga
    19681 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
    19741 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
    19801 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
    19861 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
    19921 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
    19981 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
    20041 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
    20101 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
    20161 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
    20221 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
    20281 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    20341 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    20401 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    20461 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    20521 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    20581 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    20641 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    20701 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    20761 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    20821 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    20881 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    20941 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    21001 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    21061 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    21121 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagttaaa agcttctgtc
    21181 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    21241 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    21301 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    21361 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    21421 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    21481 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    21541 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    21601 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    21661 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    21721 aagaactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    21781 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    21841 accgtcgttt tatataggaa attttctgaa aattgttgca tatttttcga aattttgtta
    21901 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    21961 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    22021 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    22081 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    22141 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    22201 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    22261 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    22321 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    22381 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    22441 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    22501 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    22561 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    22621 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    22681 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    22741 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    22801 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    22861 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    22921 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    22981 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    23041 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    23101 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    23161 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    23221 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    23281 gataaaagag gttgtgaaag agacgttcag ccttattgcc gataggctgt ctcttgaaca
    23341 ctgtttattg agaggtcgtg aaaaagccgc tcagtttcga gtattaagaa atattttttg
    23401 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23461 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23521 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23581 atttctttta taatatagat tttagacata tatatgatat aaaaatagtt ttcgcaaaaa
    23641 aa
//
LOCUS       DAZKMB010000034        23605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000034 DAZKMB010000000
VERSION     DAZKMB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.034"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..939
                     /gene="purR"
                     /locus_tag="ACG8V3_001191"
     CDS             121..939
                     /gene="purR"
                     /locus_tag="ACG8V3_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0299741.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1442..2815
                     /gene="glmU"
                     /locus_tag="ACG8V3_001192"
     CDS             1442..2815
                     /gene="glmU"
                     /locus_tag="ACG8V3_001192"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0299742.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2909..3880
                     /locus_tag="ACG8V3_001193"
     CDS             2909..3880
                     /locus_tag="ACG8V3_001193"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0299743.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3886..3988
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4054..4623
                     /gene="thiT"
                     /locus_tag="ACG8V3_001194"
     CDS             4054..4623
                     /gene="thiT"
                     /locus_tag="ACG8V3_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0299744.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4704..5477)
                     /locus_tag="ACG8V3_001195"
     CDS             complement(4704..5477)
                     /locus_tag="ACG8V3_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299745.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5744..7444
                     /locus_tag="ACG8V3_001196"
     CDS             5744..7444
                     /locus_tag="ACG8V3_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0299746.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7414..7632
                     /locus_tag="ACG8V3_001197"
     CDS             7414..7632
                     /locus_tag="ACG8V3_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0299747.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7652..8542
                     /locus_tag="ACG8V3_001198"
     CDS             7652..8542
                     /locus_tag="ACG8V3_001198"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0299748.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8560..8844
                     /locus_tag="ACG8V3_001199"
     CDS             8560..8844
                     /locus_tag="ACG8V3_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299749.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9331..9945
                     /gene="gmk"
                     /locus_tag="ACG8V3_001200"
     CDS             9331..9945
                     /gene="gmk"
                     /locus_tag="ACG8V3_001200"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0299750.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9950..10255
                     /gene="rpoZ"
                     /locus_tag="ACG8V3_001201"
     CDS             9950..10255
                     /gene="rpoZ"
                     /locus_tag="ACG8V3_001201"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0299751.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10508..12916
                     /gene="priA"
                     /locus_tag="ACG8V3_001202"
     CDS             10508..12916
                     /gene="priA"
                     /locus_tag="ACG8V3_001202"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0299752.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12931..13422
                     /gene="def"
                     /locus_tag="ACG8V3_001203"
     CDS             12931..13422
                     /gene="def"
                     /locus_tag="ACG8V3_001203"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0299753.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13415..14353
                     /gene="fmt"
                     /locus_tag="ACG8V3_001204"
     CDS             13415..14353
                     /gene="fmt"
                     /locus_tag="ACG8V3_001204"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0299754.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14353..15711
                     /gene="rsmB"
                     /locus_tag="ACG8V3_001205"
     CDS             14353..15711
                     /gene="rsmB"
                     /locus_tag="ACG8V3_001205"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0299755.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15724..16464
                     /locus_tag="ACG8V3_001206"
     CDS             15724..16464
                     /locus_tag="ACG8V3_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0299756.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16461..18530
                     /gene="pknB"
                     /locus_tag="ACG8V3_001207"
     CDS             16461..18530
                     /gene="pknB"
                     /locus_tag="ACG8V3_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0299757.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18695..19594
                     /gene="rsgA"
                     /locus_tag="ACG8V3_001208"
     CDS             18695..19594
                     /gene="rsgA"
                     /locus_tag="ACG8V3_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0299758.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19607..20257
                     /gene="rpe"
                     /locus_tag="ACG8V3_001209"
     CDS             19607..20257
                     /gene="rpe"
                     /locus_tag="ACG8V3_001209"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0299759.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20258..20896
                     /locus_tag="ACG8V3_001210"
     CDS             20258..20896
                     /locus_tag="ACG8V3_001210"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0299760.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            21051..21905
                     /gene="mreC"
                     /locus_tag="ACG8V3_001211"
     CDS             21051..21905
                     /gene="mreC"
                     /locus_tag="ACG8V3_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0299761.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21909..22418
                     /gene="mreD"
                     /locus_tag="ACG8V3_001212"
     CDS             21909..22418
                     /gene="mreD"
                     /locus_tag="ACG8V3_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0299762.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            22673..>23605
                     /locus_tag="ACG8V3_001213"
     CDS             22673..>23605
                     /locus_tag="ACG8V3_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0299763.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEE"
BASE COUNT         7941 a         4245 c         5065 g         6354 t
ORIGIN      
        1 cttcttttta ttttaataaa taccgaacta ttttcttatt ttttcataaa atgctcgaat
       61 ttttgaagaa aaacttatat aatgtaaaac gataaaattt cttaaaagga gttggaatag
      121 gtgaaagtac gtcgcagtga acgtctagtt gacatgacac agtatttatt gaatcacccg
      181 catgaattga tttccttaac aagctttgcc gaacgttatg aatctgctaa atcttctatc
      241 agtgaagatt tgggaatcat caagaaaaca tttaaagaac gagggtatgg aaccttggaa
      301 acaatcccgg gtgctgcagg tggtgtattg ttcatccctg aaatttcata tgaagaggcg
      361 aaaaaatata ttgaatctct agcagaacgt ctatcagaac aagaccgtct gcttccagga
      421 ggatatgttt atctctctga tttgttagga gaacctgaac tgctgcgtca agttggaaga
      481 atcattgctt ctaaatacct tggtgaaaaa atcgatgctg taatgactgt agccacaaaa
      541 ggcgtaccta ttgcccaagc tgtgtcctat tatctgaatg cgccattcgt tattgtacgc
      601 cgggactcaa aaataacaga aggatcaaca gtaagtgtca actatgtgtc tggatcttct
      661 gaaagaattg aaaagatgga actgtctaag agaagtttga aacgtggttc acgtgtcctt
      721 gtagtggatg atttcatgaa aggcggcgga acagtcaacg gaatgaagag cttgattgaa
      781 gaattcgaat cagaacttgt tggcatcact gtatttgccg aatcaaaatt caacggtcat
      841 cgcgccatcg atgattatac gtcactgctt tatgtcaaag atgtagatac tcaaacgaaa
      901 aatattacgg ttgtgccagg aaattatttt tcagaataag aggttgtgaa aaagccgctt
      961 agtttcgagt agtaagaaaa attttgtgaa aatggctttc cacatttttt cataaatttg
     1021 gcttaatacc gagaaactgg cttttgaaca ccgtttatct agaggttgtg aaatcagcgt
     1081 cctgtcctga gtattagctc aataaacgga aaaattgctc ctcatatttt gccgtttatt
     1141 gagctaatac cgaaggtcag ctgattgaaa aacgtttaat gaagaggttg taagcctacc
     1201 gtttaactct aagtcacaag aaacgatttc aaaaaacagc ttttcatgtt ttatgagtag
     1261 tgtgacttgt gaccgaggag ttggcaggag agcaccggtt accaaataga taggcccgtt
     1321 atggtctatc tattttttta tccagaaaac gctgggataa aagaaaatca cttgttttgt
     1381 tgaaaaaatg ggtatactgg gataaactga aatcagcaaa ctatatttag gagtgacgca
     1441 gttggacgca cgttatgcaa ttattttggc ggcagggaaa ggtacccgca tgaagtcaaa
     1501 attatataaa gttttacatc ccgtttctgg taaacctatg gtagaacata tcattaatcg
     1561 ggtcagtgaa acgaaaccag atgaagtgat tacgatcgtt ggacacggag cagaacaagt
     1621 taaagcacaa ctaggagaac gcagtaaata tgcgttacaa gcagaacaat tgggaacagg
     1681 acatgctgtt ttgcaagctg cttctttctt ggaaggaaaa aaaggaacaa cgttagttat
     1741 cagtggcgat acaccattat tgactactga aacgctgaac aatctttttg aatatcacca
     1801 aggaaaaaat gcaagtgcaa cgatcttgac ggcacaagca gaaaacccaa caggatatgg
     1861 acgaatcatc cgtgaccata ttgggatcgt agaaaaaatc gtggaacaaa aagatgcaac
     1921 accagaagaa gctcttgtac aagaaatcaa tacaggaact tactgttttg acaacgaagc
     1981 tttgttcgat gcgttaagca aagttggaac aaacaatgct caaggtgaat actatctaac
     2041 agatatcatc gaaatcttaa aagaagaagg ccatacagtt gctgcttatc aaacggatga
     2101 ttttgaagaa tcaatgggag tcaatgatcg gatcgcgtta gctaaagcaa atgagatcat
     2161 gagaaagcga attaaccaga tgcatatggt caatggcgta agctttgtcg attcagcaac
     2221 gacatacatc gatgcaggcg tagaaatcgg tccagacaca ttgatcgaag caggtgtaca
     2281 aatccaagga aatacagtca ttggttctga ttgtgtcatc gggtcacatt caaaaatcgt
     2341 agacagtagg attgaggatc acgtcgtcat tgaaaactca gtcattgaaa gcagccatgt
     2401 caagaaacat gcagatgtgg gaccttatgc acatttacga ccaaaagctg aaattggcga
     2461 aaacgtccat atcggaaact ttgtggaagt gaaaaatgct caaatcggca aaggaactaa
     2521 agtcggtcac ttgacttatg ttggtgatgc tacgctggga gaagaaatca atgtcggatg
     2581 tggtgttgtc ttcgtcaact atgatggaaa gaacaaacat catacgactg tcggcgacca
     2641 cagctttatt ggttctagca cgaatatcat cggacctgtc gaggtagcta aaaacagttc
     2701 gattgctgcc gggtcaacaa ttacagacaa tattccagaa tatgcgctgg caattgcgag
     2761 agcaagacaa gtcaataaag aaggatatgc aaaaaaactc ccttatctaa actaagactt
     2821 ttgcaaaatc acttgattta tttaaaaaat atgactacta tttattaggt agaattctag
     2881 agttgtgaaa aagaaagtgg aggtccccat gtcaaaacat tactttgacc caagattaaa
     2941 aatttttgcg ttaaattcca atcgcccatt agcagagaaa attgcagcag cagtcggcgt
     3001 cgagttagga aaatcatctg ttactcaatt tagtgatgga gaaattcaag taaacattga
     3061 agaaagtatt cgtggatcgc atgtctatgt tattcaatca acgagcagcc cagtcaatga
     3121 taacttgatg gagttgctga tcatgattga cgcactgaaa cgagcaagtg caaaaacaat
     3181 caacgtagtc atgccttatt atggctatgc gcgtcaagac cgtaaagcac gtgcacggga
     3241 accaatcaca gcgaaattag tagcaaacat gattcaaaaa gctggtgcaa caagaatgct
     3301 gactttggac cttcatgcag tccaaatcca aggtttcttt gatattccag tcgatcattt
     3361 gatgggtgcg ccacttattg ccaattattt cttagaaaaa ggaatcaaag gcgatgatgt
     3421 tgtcgttgtt tcaccagacc atggtggtgt cacacgggca cgtaaattag cggaattttt
     3481 gaaatctccg attgcgatca tagacaaacg ccgtccaaaa gcaaatgttg cagaagtaat
     3541 gaatatcatt ggacaagtca aaggcaaaac ttgtgtactg atcgatgata tgatcgatac
     3601 agcaggtacg atcacattgg ctgccaatgc tttgaaagaa gctggtgcaa ctagtgtgta
     3661 tgcttcatgt acacatccag tattatccgg ccctgcttta caaagaatag aagattcagc
     3721 tatcgagcgt ctagttgtta cagactcgat ctatcttcca gaagatcgta aaatcgacaa
     3781 aatcgatgaa gtaagtgttg gcggactaat gggagatgca atcaaacgta tccatgaaaa
     3841 caaaccagtt agtccattgt ttgaaacaaa aaataaataa taaataaact cacaaagggg
     3901 agtccaattg ggctgagatt gaatctattt ctaaaccctt cgtacctgta tcggttatgc
     3961 gagcgtagga attgtgaata aacagctgtt tgactatttt cgaaagctgc ttcctccttt
     4021 gtgacttttt tattcaaagg aggaagtttt tttatgggaa aaaatagagt ttgggtagaa
     4081 ggaacgattg ttgcagcgtt agcgatggtg ttgtcgttga ttccgatcca gatcggcagc
     4141 agtttttcta tttcattagg gcagattcca ttgactcttt ttgcgttaag aagagggaca
     4201 aaggcaggga tgctggcagg tttcatttgg ggcatcctac attttccgtt gggacaagtt
     4261 tattatttaa gtgtcgttca agtgttgatt gagtacccga ttgcctatac ttttgcaggc
     4321 gcagcagggc tcatggcttc aaatgtccaa aaaggctatg tagacgataa tacgaaacaa
     4381 attagaaaaa gcatcgtgtt tggtgcattg attggcgcac tttccagata tttttggcat
     4441 tttattgcag gagtggtttt ctggggagcg tatgcactat gggggatgaa tccttggctc
     4501 ttttctttcg taatgaatgg tgccagcggg ttgagtacag ctattgtaac gatcatcgtt
     4561 ttattaggga tccaacaaac gactccttcg atttttgtgc cagataaacg acaggagctg
     4621 taaataaaaa agaagcaaga aacttgagac agaatcatgt cctagtttct tgtttccttt
     4681 ttattttttg ataagaaata gatctacttc aatggcagat aagaaagatc ggcatcttct
     4741 accaattgcc agtgtcgatc ggtgacagag tagattaaag tagcgatgat ttcctcttct
     4801 cgataccggg tgccttttaa tttccaaata taatcttcaa tcacatagat tgggatacga
     4861 tatttaccat cgatcaaacc aacttgatca aaagaaaaaa tgatcccttc ctgcatcaaa
     4921 ttacgcattt ctttcaataa ttccacactg attttttcaa caggtgtttc ttttttgaaa
     4981 aaagattttt ttacattgat cttatctgac aagatcgaac ttaatttact agcaaaaatc
     5041 ctataaaatt catctaaata gcgataataa atccgattca gctgatcatt aagatcgaag
     5101 tatacaaaat tattctgtag tttataaaaa aacggcgaat gaagatgtgt tttcatatga
     5161 ccaaaatata ataattctga aatttctaaa ggtgtcaatt ctttcagcat cgttaaatca
     5221 gtaaagtcga tccacttcaa ttcatcgaat ccacgcctgt tacgagaaga ggaaaagaaa
     5281 cgctgaatat tttctgtacc acggattact ttcagccctg tatgggattc atattctcca
     5341 gcagaagcag atggatcaag caagagcaag ttttgaggaa aatgcacgat actgtttgca
     5401 aagtcagtag cagtcagccc ttttgttaaa acagcattac ttgtggtatc aatatgtaca
     5461 taaactgact cagtcatcat gttcctcctc ttatttcctc ttaatctctt ttattttaca
     5521 acatttgcat taaaaaagct tgtttctttt attttaaaat tccattacaa aatcacgttt
     5581 tcattgaaaa cactctcatt ttcaattaat ttttgtgatt tttgtatttt tttatttaaa
     5641 ggaggtttta tgaattgttt tcttatggaa tctacaaagt cgaatacgag actcatgacc
     5701 tttttatgac gtactggtat acttgaagaa aaaggagagc gcgatggaga taaaaaaagt
     5761 ttacttttca cagaaaatgt cgctgtattc gcctatccag caagcaaaac aaccccttcc
     5821 attttttcaa agtcatcaag aaagtagaga cgctttttgg cgttctgcaa aaaagcaatg
     5881 tgcttttaaa gcagtatcga aaaaatatac cgtctatttt actattgcta aaagtagagg
     5941 agatgttatt tttaataatc tgttgattga aggtgaaact tttgaatgga aaaaattttt
     6001 tcggtatatg gaagcttgcg ctcgtttttt tattaaagaa aactgttgtt ttttatttca
     6061 atctcctcta tcagaagaat ggttgcgtat ttttcataaa aacggctatc aaggaaccac
     6121 acaattaaat aaaaagctgg tctatcatac agcacttgtt ttaggtggag gaggagcgca
     6181 cggtgcttat cagattggtg tgtggcaggc gctgaaggaa catgatatca actttgagat
     6241 tatcacaggt acctcggtag gtgcgttgaa cggcgcattg attttacaag gagatatgaa
     6301 aaaagctgtg aatttatgga aaaagctgtc gacaagacaa gtgcttgcgt taccagaaat
     6361 ggctgcagtt gaagaccttc gtgaacgctt ctatcgagaa agacgccaaa tgacgaaaac
     6421 agcactaatt gaaggcgggg tttcttctgc tccattggaa aagatggtca aagacaattt
     6481 ggatttgtcc ttactcaaac ataatcccaa aatacgttta tatacggtgt ctactaaact
     6541 gccagaacta gcggaagtag tgactgatat ccaacagaca aaaacagaag aaataccaga
     6601 ttggatactc gcctctgctt cattttatcc cgctatggct tatcgaaaga tcggaaaaaa
     6661 taaatatgca gacggtgggt atcgcaacaa aatacctatt gatattgcga ttaacaaagg
     6721 agcaacagag gcatttgtcg tagatgtgca agggccaggc ccagcgaaaa aaattcgtat
     6781 gccagacatt tttatccatt ggaaatgtca aacattgtgg acactaggca gctttctgct
     6841 ttttgatagc caaaggaacc aattaaattt acaattaggc tatctggaaa tgaaaaaacg
     6901 attaggctat tactatggga actggtatac atttcattct gttaaacctg caagaacata
     6961 ctggcgagga tttttgtctt acttaacaaa agaaattcag ttagaattat cttttttcaa
     7021 aagcataaaa ttttggcaaa aactgcgtaa tttatataag aacagggtcg taccagagac
     7081 atgcggattg gcgatgctag aacttttagc aaaaaaacat tttttactgc ccaatgaaat
     7141 ctatcaagta gacatgatga ttcaaatgat ctgccaacaa gatataaaaa gtatgccgga
     7201 tgatttattg gcacaaattg gtcaattaag tactgaagaa tggcgaaggt atcgaaaata
     7261 ccagcaaaaa atacaaaatg agcgcacgaa aactgtttat tttgattatc tgctacaagg
     7321 aaaacgcaag gatcgtttgc agcatgaatt gcttaagcaa ccagttgaca cattactgat
     7381 cctatattta tattatctca aggaggaaca accatggcac aagaattttc atatgaaatc
     7441 gtagaagaaa tcgctatatt gtcagaaaac aacaaggggt ggcgtaaaga actgaaccta
     7501 gtcagctgga atggtcgccc gccaaaattc gatcttcgag attgggcgcc agaccatgaa
     7561 aaaatgggga aaggcctgac attgacgaac gaagagtttg aacaactgca aaaagcaata
     7621 gaaaatatgt aatgaggaag gaccctgtcg aatgaaaagt atgactggtt ttggcaaagc
     7681 catccgcgaa acaaaagcgt atcagttaga tattgaaata aaaagtgtca accagcgttt
     7741 tctagatgtt caaatacgaa gtcctaaaat cctgaacttt gttgaaaacg attttcgtca
     7801 gcttattaaa cagcagttaa gccgcggtcg aatcgaagta tttgtgaatc tagcttatat
     7861 tggcaaaaac gaaaaacaga tcgtcatcaa ctgggaattg attgattcac ttatcactca
     7921 attgacagac ggaattcaac aacgatacgg tagtcaaaat caagtgtcaa ttagtcggat
     7981 ggcggaacgc ttcgtgctgg acgaaaactt cgtaacggta gaagaaaaac cggaagagag
     8041 catggaagaa ttggcacagt tagctctaga tacattgaaa agcgcacttg attctattga
     8101 ggcgagtcga gaaaaagaag gtcaagcttt agctttagtg ttgaaaaaga atactgaaga
     8161 attcaaagaa gtactaaaaa aactcgatag ttttgtagaa atctatgaaa aggaatttca
     8221 aataaggtat caaaaaaagc tggaggaatt tcttggagcg aaagtcgatg agcaacggtt
     8281 gttgacggag ctagccatct tgttggaacg cggagatatt cacgaggaac tggatcgtct
     8341 ggcgatccat gtacaaaaac tagatgaact tatcgaagca caatgtcctg taggtcggga
     8401 attagatttc ctgatccaag aaatgaatcg ggaaatcaac acgatcggtt ccaaatcaaa
     8461 tgcaatcgaa attaaaaatc aagtcgtcca gtctaagacg atcttagaaa aaatcagaga
     8521 acaagtacaa aatattgaat gacgaaagag gtagagataa tgaaatatta tgacgtaacg
     8581 ttccacgaat taagtggaaa aactgtggtt aaacgggata tcccttctga aaaaaacggc
     8641 tttgacgtat ggaaagatgc ctgtgcagac tataatgaga acgaactgtt tattttgatc
     8701 aacgacggtg cttacgtgac gatgaatcgg aaatttattg ttcgaattga cacagaagag
     8761 gtagaagatc cgacagaaaa agcacgttct cgaaaagatg agatcatggg tgtagtgaat
     8821 actttgtcca atatgggctt ttagcaagga tgctgaacaa agaaatacaa taaataaaca
     8881 gaattggatt tcttcgctta gcttctttgc tctaggaata catttaaagg ttgtgacaaa
     8941 attcgtgtca caatcttttt ttattttgaa taagtgatgt tcaaaaacca ttttctcggt
     9001 attaagccaa atttatgcaa aaatgtgaga aactattttc acaaaatttt tcttcatact
     9061 cgaaactaat cggcttcttc acgacctctt atacacggcg ttcaaaagct tgcatctgcg
     9121 ataataaccc caaccaaacg aaaaacatga gaagctgttt atgtttggct gttcttatta
     9181 cttgatgcag gacagctttt ttacgacctc ttggtacagg gtagcttttt taccaatttc
     9241 tagctataat taaagataac cttgaaagtt gcagaaaaaa agtgcaaaat aaacgataag
     9301 tacaaaaaga ctagttgaaa ggacgtcgtc atgtcagagc gtggattatt aatcgtgttg
     9361 tctggtccat ccggtgtagg aaaaggcaca gtacgaaaag caatttttga gagtgaggat
     9421 aatgattttc agtattcgat ctcaatgact acccgtaaaa tgcgtgaagg ggaggtcgaa
     9481 ggcgttgatt atttcttccg aagcaaagaa gagtttgaat caatgatcga agctggagag
     9541 atgctggaat acgcagaata tgtcgggaat tattatggaa ctcctttgtc atatgtcaac
     9601 aagacattgg atgaaggaaa agatgttttc ttggagattg aagtccaagg agcaaaacaa
     9661 gtcaaagaaa aagtgcccga tggtgtattc atctttttga ctccgccaga tttagcggaa
     9721 ttaaaatcac ggatcgtagg ccgaggaaca gatgcagatg aagtaatcga aaaacgaatg
     9781 aaagttgcaa aagaagaaat cgaaatgatg gctctttacg actatgctgt tgtaaatgat
     9841 gaagtgccgc tagcagttga acgaatcaaa aacattattg ccagtgaaca ttttcgagta
     9901 gatcgcgtaa tcggtaaata tattaaaatg ttaaaggaga tgtagtctta tgatgttaaa
     9961 accttcaatt gactcactat tagatcgtgt aaattcaaaa tattctctag tgattttagc
    10021 aagtaaacgc gcgcatgaat tggatgcaag tgcacagcct actctagatt cttttgattc
    10081 agtaaaaagc gtcggtcaag ctttagaaga aatcgatgca ggaaatgtag tcaatgatcc
    10141 acatcctgaa ttaaaacgtg aacgtttgaa gatggaagaa gaggaacgtc aagcacaaaa
    10201 agagcgtgaa caacacgaat tggaatcacg tatccgtgaa gagcaaaaaa tgtaataaag
    10261 aaaaatagag cgaacatcta aaaagaaaag caaacccagc tgtttccttt tcaaacattc
    10321 gccacttatt gtctggtaga aaaggctgtg tctgaacttt ttgacacagt tctttttttt
    10381 cttatctgct gttaattttt tgttaggatt tggcagaaga taagacaaat ccatcgatct
    10441 ctagtaaaat aggcaaggaa ctttttgtcg cttgataaga aaagacaaaa aaaggaggaa
    10501 acgaaccatg acattaacag ctgaagtcat tgtggacgta cctactatgc agaccgatca
    10561 gccattcact tatttggttc cctctgaaat ggaaacagct ttgcaagtcg ggatgcgagt
    10621 agaagtccct tttggtaatg gcaatcgtca tgtccaagga tttgttatgg ctatccaaaa
    10681 atcagaagaa tcagctaatc catctttgaa ggcagttatt cgtttgctag accttgctcc
    10741 ggtggtcaat gaagaattac tttcattagc tgattatatg aaaaaaatta cttacgcatt
    10801 caaaatcact tgtctacaaa cgatgcttcc gagtgtgatg aaagcagaat atgataaatt
    10861 gatctatcct ttggcagaca caccagaagt tcaggtattg tttgatcagc gagaagtgat
    10921 ttcctggaag gaagcagaag aagaaggcag cctttctcaa ttgattcgct ggcgccaaga
    10981 gcaattagta gatatcaaat atgaagtcca tacacgaaat aaagtaaaaa cgattcgttt
    11041 ggtccgttct ttgttaactg aaaagcaaat cgaagaagaa tgggcaaaat tacggcaaaa
    11101 tgccaagaaa caaaaagaac ttcttttgtg tttatctgaa ctgagccaag aagaaccaat
    11161 tgcttatttc aaaaataaag gaatcagtac tgctgtcttg aatcaaggaa aagaaaaggg
    11221 ctggctggag tttatagaat cagaacgtta tcgcgaccct tataaagacc gagtatttga
    11281 ccaaacgacg gcattggaac tgaatgcaga acaaaaaaat gctgtcgaac aaatcatcac
    11341 agcaggtcag cagcaaaaag atcaggtctt tttgctggag ggaatcactg gcagcgggaa
    11401 aacagaagta tatcttcagg caatcgctga tgttttgtca gaaaataaaa cggctattat
    11461 gttagtgcct gaaatcgcat tgactccgca aatggtcgag cgattcaaag gacgcttcgg
    11521 agaatcagtt gcggtcttac atagcggact ttcccaaggt gaaaaatatg atgaatggcg
    11581 caagattgag cgggaagaag cgcaagtagt agtaggcgca cgatctgcta tttttgcccc
    11641 gctgaaaaat atcgggctga tcattatcga tgaagaacat gaatcaagtt acaaacaaga
    11701 tgagacaccg cgttatcatg cgagagatct agcaatttgg cgcagtaaat atcatcattg
    11761 cccaatagtt ctaggaagcg cgacgccctc tttagaatca agagcacgag ctcaaaaagg
    11821 cgtttatcag ttattgcagt taaaccaccg agccaaagca gctgcacagc ttccagctat
    11881 tgagatcgta gatatgcgtg aagagtttca gaatcatcgg acctctactt tttctgctaa
    11941 tttgcaggaa aaaatacaaa atcgattaga caaaaaagaa caaactgtcc tactgctcaa
    12001 tcgtcgagga tattcgtctt ttgtgatgtg tagggactgt ggatttgttc ttccatgtcc
    12061 taattgcgat atctctctta cactgcatat ggatacacgt tcaatgcgct gccattattg
    12121 cggacatgaa gaacctattc ccaatcgctg tcctaattgt ggcggcaata aaatccgcta
    12181 ctatggaaca ggtactcaaa aagtagaaga ggaactccga gaactttatc cgcaagctag
    12241 gattttgcgt atggatgtag ataccactcg aagaaaaggg gcacacgaac agattttaca
    12301 aaaattcggt gcaaaagaag cagatatatt attagggaca caaatgattg ctaaaggttt
    12361 ggactttcca gaagtgacat tagttggggt gttgaatgct gatacctccc tgaatttacc
    12421 ggattttcgt tctagtgagc atacattcca attgcttacc caagtttcag gacgagcagg
    12481 gcgagcggaa aaagcaggcg aagtcgtgat ccaaacattc aatcctcaac attacgccat
    12541 agaattggct aaaaaacaaa attatgagca gttttatcag caggaaatgc atgtacggca
    12601 tcgtggaggc tatcctcctt attactttac tgtgaaaata accgccagcc atcctgaaga
    12661 acaagtagcg gcaaagaaaa tctttcagat tgccaatcag ctaaaagaag tgttaagccc
    12721 tcaaagcttg ctattagggc caacgcccag catgattttg agagtgaaaa accgatatta
    12781 ttatcaacta atcatcaaat ataaacatga accgaatctt cctcatgttt tggatgaaat
    12841 tttgaatgga agccagaaag aacagcgaca aggtcttttt gtggcaattg acaacgaacc
    12901 attaaacttt atttaagaaa gggccaaatt atgcgctatc ctatattgat ccaccctaat
    12961 gataaattaa aaagaactgc tcagccaatc gatgtaatca cggacgagac aatcgcttta
    13021 ttagacaatt tatatgagac aatgattgcg aatgacggaa tagggatcgc tgctcctcaa
    13081 gtaggacaaa acaagagaat tgctgtgatc gaagtagatg aaggcgagaa atttgaactg
    13141 attaacccag aaatcatcga agcaaaaggt gaaagccttg acgtagaagg ttgtctcagt
    13201 attccgcatg tctatggaac ggtcaagcga gcagatgaag tgaccgtgcg ttattatgat
    13261 cgagacggag aagagataga agtgacggct tttggctatt tagcccgtgc cttccagcat
    13321 gaaatcgatc acttagacgg tatcttgttc attgagaaaa tgatacagca gatcccggaa
    13381 gaagaacttg aagaatatat ggaggaacat cttgatgact aagctagtat ttatgggcac
    13441 acctgctttt tctgtgccga ttcttgaagg gctgatagaa tcaggttacg agatccaggc
    13501 agtggtaaca cagcccgacc gtccagttgg gcggaaacga gtaattacgc cgacaccggt
    13561 aaaaaaagcg gctctgaagc acggtatccg tgtgctgcag ccagagaaaa tctccggttc
    13621 gccagaaatg gaagaaatca ttgaattggt gcctgatgtg atcgtcactg cggcatttgg
    13681 ccaattcttg cctgaaaagt tattgcaagt accaaaatta ggggcaatca atgtccatgc
    13741 ttctttactt cctaaatatc ggggaggtgc gcctgttcat tatgccatca tgaatgggga
    13801 gaaagaaact ggtgtaacga tcatggaaat gatcaaaaaa atggatgcag gcggaatcta
    13861 tgcgcaagaa agcataccaa tcactaaaca agatgatgta gggacgatgt ttgaaaagct
    13921 tagcctttta ggaagaaagc tattgctaga aacattgccc aatattttgg atggacaaaa
    13981 accagtacct caagacgagt cagaagcaac tttttcgcca aacatcacac gggaacaaga
    14041 agcaatcgat tggcataaaa ctgcagaaga aattgacaac caagtacgcg gaatgcgtcc
    14101 ttggccaatt gcattcacga cctatgaggg catacgctgg aaattattat cggttgaagt
    14161 attggcagaa actacagaca aagcacctgg tacgatcatc cggaaagaca aaaaaaatct
    14221 atggattgct tgcggtaaac agaccgtctt agccatcaat gaattacagc cagctggtaa
    14281 aggaaaacag gccgtacatg atttccttaa cggaagcggt cagcaagttc agattggtca
    14341 gcaggtgaaa taatgaaaaa aatccctaaa aaaatgaaaa aatcagtacg atatatggca
    14401 ttactgacat tagagcgagt gaacaagggt ggcgcctact cgaatctttt gttgaatgag
    14461 atgatcaaac aaagcgagct aaatcaaaaa gatgtgggtt tatttacaga attgatctat
    14521 ggtacgatta gccatcagcg actattggaa ttttacgttg caccattgat cgctaaagca
    14581 aaaaaagtgg atgactgggt caagatgctt ctttatcttt ctgtatacca attagaattt
    14641 ttagataaag tccctgcaca tgctatttta aacgaggcgg tcgaaatcgc aaaagtaaaa
    14701 ggaaatcctg ggacagggaa atttgtaaat ggtgtcttga ggaattatca gcgtcaagga
    14761 gcaccagatc tgcaaggcat tgctgatcct atcgaaagac tttctgtcaa aatcagcctg
    14821 cctctatgga tgacgaaacg actggtggat caaatcggat acgaagaaac tgaaaagctc
    14881 ggtttatcgc tatatcagcc tagccatgct agtgcccgag tagatacacg ccgactttcc
    14941 agagatcagg ccatcactgt tttgcaagaa gaagagatca cagctgaaaa aagccaagtc
    15001 agtccatacg gtatagtagc gaaaaaaggg catttagctt ctagctcatt gttccacgat
    15061 ggcgtcatga cgatccaaga tgaaagttcg atgcttgtag cgccgtctat gcaaatcgag
    15121 cctgaacatc atgtcttaga tgcctgtgca gcacccggag gtaagacgac gcatattgct
    15181 tcttttttag atccgtcatg cggtggagaa gtggtcgctt tagatgtaca cgaacacaaa
    15241 gtcaaactca ttgaagataa tgcagcacgt ctaggtgtct cttctgctgt ctacgctcaa
    15301 aagatggatg ctagagaagt cagtaaacag ttcgaagcag agcagtttga tcggatttta
    15361 gtagacgcgc cttgttcagg tcttgggctg cttcggcgaa aaccagatat tcgttataaa
    15421 aaacagccgg atgaattagc gaatttacca aaaatccaat tggaaatttt agaaagctgc
    15481 gcaccaactt taaagtcttc cggtattctg acttatagca cttgtacgat cttaaaagaa
    15541 gaaaaccaag acgttgtttc tgctttcttg gaaagacacc ctgattttga gaggattgat
    15601 gttttgacca atcctatgct acaatcatca atcgaagaga aaatgttgac tttgtatccg
    15661 catcagtttt acacagatgg atttttcatt tgttgtttgc gcaaaaagta atgaggtgaa
    15721 aaaatgcaga ttgaatatca atcagatgta ggaagacgac gtaacacaaa tcaagactat
    15781 gccagtgtat ttaccagccg ttcaggtctc aagcttgctg tattagcaga cgggatgggt
    15841 ggtcatcgtg ccggagacat tgccagtcag atggcggtaa cgaatctagg cgaagcttgg
    15901 gaaaaacaag aagctgttga cgatgaaaaa atcgcccaat ggttcattca atcgattcaa
    15961 gaagaaaata tgctgattta tcagcgtgga caagaacagc cggaatataa cggtatggga
    16021 acaacaatcg tagcggccgc tctgtcagat gactggttca cgattgccaa tgttggggat
    16081 agcagagctt atttgattcg tgaagataaa atcatgcaat tgacggagga tcattcttta
    16141 gtcaatgaac tagtcaaatc aggagaaatc agcgaagaaa tggcagtgaa ccatccaagg
    16201 aaaaatattt taacacgttc tgtcggcatg cctggaacag tcgaagtaga tgtttctact
    16261 catctttggc aactaaaaga tcgactgctg ttatgttcag atggcttgac gaacatgctg
    16321 tctgaagaag aaataggaac gatcatcaat caagaaggcc cattgtctca taaagtaact
    16381 gagctgattg atcgtgccaa tgaagcaggc ggagcggata atatcaccgt tcttcttatc
    16441 gaatataagg aggacagtgt atgatcgagt tgggcaaaaa attaaatggc cgttatcaaa
    16501 tcactggaaa tatcggtagc ggtggtatgg caaatgtatt tttggcgcac gatttaatct
    16561 tggatcgtga tgtagcagtt aaagtcttac gatttgactt tcaaaatgac cagactgcga
    16621 ttcgacgatt tcagagagaa gctttagcag caacagaact cgttcatcca aacattgtta
    16681 gtgtttacga tgttggtgaa gaagacaaca tgcagtatct ggtgatggaa tacgtcaaag
    16741 gaatggactt gaaacgatat atccaaacac attatcctgt tccttatgaa acagcagtaa
    16801 atattatgca gcagatcctt tctgctattt ctttagctca taaccatcaa atcatccacc
    16861 gagatttgaa accccaaaac gtactgatcg ataatgaagg tgttgtaaaa atcacggatt
    16921 tcgggattgc tatcgcttta tctgaaacat cgatcacgca gacgaatacg atgctaggat
    16981 ctgttcatta tctgtcccct gagcaggcca gaggaagtat ggcaacgaag cagtcagata
    17041 tttatgcttt agggatcatc ctttatgaga tgttgacggg tagcgtaccg tttgatggtg
    17101 aatctgctgt aacaattgct ttaaaacatt ttcaagatga tcttccatct ataaaagcac
    17161 ttgatccaaa tgtgccacaa gcactggaaa acgtgatttt acgggcaacg gctaaagaac
    17221 ctgcagaccg ttataagtca gccgaggaaa tgtctgatga tctttctaca gtattatcac
    17281 cagctcgtgc aaacgaagaa aaatggcagc ctcatgtgat ggataatgaa acaaaagtta
    17341 tcacacccct tacggaagat gcaccaatgc ctgattcatt caaatctatg cctctgccaa
    17401 aagacaagcc agaaaacgaa accttaccag aggaggaaga agttctgcct gatcctaaaa
    17461 agaaaggaca gaaaaaatgg tggctgatcc tattagctat tcttgctgtt ttaggaatcg
    17521 gaacagctgt attctttgct tctggcgggc gaggagaagt tgttgttcct gatgtttctg
    17581 atctttctga agcttctgct agagaaacac tcgcaaaagc gaaattagaa gtagcaacaa
    17641 aaacagaaga aattgcagat gacaaaatcg aagaaggtaa tgtagtgaag acagacccgg
    17701 cagcgggaac gacagtcaag caaaaacgcg aagtcactct gtatattagt agcggcacga
    17761 aaaaaatcaa attagatgat tacagtggta aaagttatga agatgccgaa gcaagtttga
    17821 aagaattagg gttttctgct gacttgattc aaaaagaaga ggaattcagt tcagaggtag
    17881 aaagcggaat gatcatttcg caatctccat cagaagatac agaagtcgat cctaaaacag
    17941 acacgattac tttcacagtc agcaaaggac cagaaacagt gactttgagt agtcatgtag
    18001 gagaaaacta cgaaactgtt cgtaattggc ttgttacaca aggattcaat atcaataata
    18061 ttagtgctac ttatgactat agcgacactg taccatcagg ttcgatcatc agtcaggatc
    18121 cagcctcggg tgaagttgta gcagaagaaa cttacatcaa ttttgtcgta agccaaggaa
    18181 cagagcctaa attgaatgat atctccggct atacaaaaag cgaagcacaa agttatttag
    18241 ctagtgtcgg tgcagaatat atcgggcatg agacttccga atattcaaac acggtggata
    18301 aagataaagt gattcgtaca gtaccagggg caggaacgac aattaccaaa ggaatggttg
    18361 tgaacgtaat ttactctaaa ggaccagatc cagcgtcttc tagttctgaa aaagactctt
    18421 cagacagttc tagcagctca agtagctcca gtagcacaag caactcttca aaagaagaat
    18481 cttccagctc cacaagttca tcagctagca cctcagaaag ttcaaaataa gtgctgaata
    18541 aaatatatca gaagggaatg cgacattgat ttgttgcatt ctcttttttg tgtttctatc
    18601 tcttttgatc aattttcaag ccatatactt tgagaagttt gcatggaata acacctactt
    18661 tcctcttcgt atggtaaaat aaacaaaaac ggaggtggca cttatcaaag gacaaatcag
    18721 aaaagcaatc agcggttttt actatgtata cgcaaacgga gagacctatc aaacaagagg
    18781 aagaggaaat ttccggaatc ggaaaatcac gccgcttgtc ggagatcagg tgatctttga
    18841 gagtgaaaat caaacagacg gctatcttct ggaaatcttg ccgagaaaaa atcaattagt
    18901 tcgcccacca gtcacaaatg ttgatcttgg aatcattgtc acaagtatcg tagagcctgc
    18961 attttcttat aatctacttg accgctttct agttaccttg gaatacgagt cgatcgaacc
    19021 gattattttt ttgacaaaga ccgacttgtc agacgaccca gaaaaaatcg aagagatcaa
    19081 gcaaacctat actaaaatag gctatccggt gattgtaccc aatcatacgg gtgatacgac
    19141 agaactgatc cgttacttcc cagaacggct atcggtcttt atgggtcagt ctggagcagg
    19201 aaaatcgacg ctactgaatc aaatatcacc ggaactgaat ttagcaacag gagagatttc
    19261 tgatgcactt ggaagaggac ggcatacaac acgtcatgtg gaactgattc ctatctatgg
    19321 cgggttagta gcagatacac caggatttag ctcgattgac tttttgacaa ttgaagcagg
    19381 agaactgccg agacaatttc ctgaatttgt agaagcttct gtccattgca aatttagaga
    19441 gtgcatgcat gctaaagaac ctggctgcga agtaaaacat agagtagaaa caggtggtat
    19501 cgcacagaca cgttacgaca attatttgca attcttacaa gaaatcgaaa aaagaaaacc
    19561 aatgtatgca aaaaaagaag gtaaacgaaa ataggaggaa acaagaatga agatagcacc
    19621 atccattttg agtgcagatt ttgccaattt acaaagagac attgagttag tggaaaaagg
    19681 aggcgcagat tacatccacg tagatgtaat ggatggccga tttgtcccaa atatcacatt
    19741 agggccaaat attgttcagg caatccgtcc tgttactaaa ttaccgttag acgtccatct
    19801 gatgatcgtt gatccagaaa actacattcc agcatttgca aaagctggag cagatatcat
    19861 tactgtccac gtcgaagcaa ctcctcatat tcatcgggca cttcaaatga caaaaagctt
    19921 aggagttaaa gctggagtag ttatcaatcc aggaacacca gtctccatga tcaaacatgt
    19981 tttgcctttg gctgatcaag tactagtcat gactgtcaat cctgggttcg gcggacaaag
    20041 ctttattgaa gaaacagtag aaaaaattac ggaactcgct gatctacgta gagagaacaa
    20101 ttggacttat gcaatcgaag tagatggcgg aatcgttcca gaaacagcaa aaatctgtaa
    20161 agatgcaggg gcggatgtct tcgttgcagg atcatatatc tatggagccg aaaatccagt
    20221 cggacaaatc gaacgattaa aagaggcatt agactaaatg aaaattttat tagctgctgg
    20281 aggtaatccg caaaactggc cgcagtctct gctgtccaaa tacgatgcct atgtcgggat
    20341 agaccgtggt gcgctgtacc tgcttcaagc aggctaccca gtagatctgg cagtgggtga
    20401 ttttgattca ttgtcgaacg aagagcagaa ggaagtattt gcaaaagcca aagagtatac
    20461 ccgttcccct gcagaaaaag acgatacaga tacacagtta ggtatcttaa aaacatttga
    20521 acgttacccg caagcggaag tcactttgat tggagctacg ggcggaagat tggatcatct
    20581 tttagccaat ctctggttag gtatggaacc tcgcttcaaa ccctttgccc atcagcttat
    20641 tatcagagat tgtcaaaatt atttgtctta ttatttgcct ggagagcata tagtaagaca
    20701 gataccagat atgaagtatt tagcttattg ctgcttgact cccgtctcga atcttacatt
    20761 aaaaggcagc aagtatgtat tggaaaataa agaagtagaa tatccgacat cttatgcaag
    20821 taacgaattt ctttcagata ccgcagaatt ttctttttca gatggtatga ttgcagttat
    20881 tcaaagcaaa gattgatcca taaacaaata aaaaaatttg tctttcaggc taaaaatgac
    20941 agaatctatt atttgctaaa aaaaagctta agaggagcaa cttacctttt gtttcacggc
    21001 atttagtgat aaaattgaac acaagagtct aattaattcg aggtgtcttt gtgaagaagt
    21061 tcaatcctaa taaaaatatt attattacct tggttattgt tatcatcgtg gtgacgatac
    21121 taagtgtgac cgctgcgcag cgagcgaatg aaggaaagcc caatttcttc caatccatcg
    21181 ttaatgacag tgttgcaatg gttgataaaa caatctctgc cccagtacgg tgggtacgaa
    21241 atggagtaga ttctgtccat aatctttttt caacttatgc cgaaaatgag cggctaaaag
    21301 aaaaaatcga tagctatgat gacgtttcac agcaaaacaa gaattacgag agagaaatcg
    21361 aagcgttgaa aaaagaactt gagttgaatc agacgctgac tagttacgaa aaagtaacgg
    21421 caaatgtcat tactcgttcg cctgacagtt ggcaggacat gctgatcgta gacaagggtt
    21481 ctaaagacgg tatcgaagca aatatggcag tcatgtcgca aaaaggattg attggccgag
    21541 tgatcgaggt caatacggct tcgtctaaaa tcgaattact gtcatcctct aatgaaagct
    21601 ccaatcattt tccagtacgg gtatcttcgg ctaatggcga agcgtttggt ttgcttaaaa
    21661 actatgatga aaagctccat gccttagtgg tgacccaatt aactggtgat acggatatca
    21721 aagaagggga tgttgtccag acatccggtc ttggagggaa ttctccagct aacttgccga
    21781 tcggtacggt tattaaaacg aaaccagata gttatgggct ggatcgggaa gtttatgtga
    21841 aaccttatgc agaaatgtat gacgtgtcag ttgtgacgat tgtccaacgg ctagcggagg
    21901 attagtccat gttgaagaaa gaaacaatga agtactatct gccaatcgtt ttgttctttt
    21961 tgatgttgat agatggtcat ttaacaagaa tgctagggga gtggtcgaaa ggcacctata
    22021 tgtcaaatgc ccactttctg atattggcat tattatgttg cagtatggcg tttgaaaaac
    22081 gttatttact gattaccacg attgttctcg gggctatcta tgacgcttac tatattggcg
    22141 ttatcggtat ctatgcagta gctctccctt taattgtatg gttgatgtat gtaatgaaag
    22201 acgttatcca tgtcaacatc tttactgaat ttttcagtat gatcatcttt gtcacgggtt
    22261 atgaattgtt tacgatggtg gtccagttga tttttaaatt agcagtagta aataacacgt
    22321 attttattac aaggttttta ggacctacac tgctgttgaa catgattata tttgtattat
    22381 tcatttttcc ctttaagaaa ttattcagtg atgaataatt tcttttttta tttttaggtc
    22441 gaaagcgaaa aagaatcgtt gttatcttaa gaaatgctag acttaacgcg gctttttcat
    22501 gatcattttc tgaaacgaac agttttgtca tttttaaaag ttgttttgaa acatttttgt
    22561 aacataacta ttatatcgtt gacacactgc tatggtacaa tttttatatc gttaagaaag
    22621 acttaaatat atttagatat ttattaaatt aagccagtcg gaggaatgaa gagtgaaaaa
    22681 gagtttaata tcagcagtaa tggtatgttc aatgaccttg actgccgtag catcaccgat
    22741 cgctgccgcg gcggacgatt ttgattctca gatacaacag caagatcaaa agattgcaga
    22801 cttgaaaaat caacaagcag atgcacaatc acaaatcgat gccttagaaa gccaagtttc
    22861 tgaaatcaac acacaagcac aagacctatt agcaaaacaa gatacattgc gtcaagaatc
    22921 tgcacaatta gtaaaagaca ttgcagattt acaagaacgt attgaaaaac gtgaagatac
    22981 aatccaaaaa caagcacgcg aagcacaagt aagcaacaca agctcaaact acatcgatgc
    23041 tgtattgaat gctgattcat tagcagatgc tattggacgc gttcaagcaa tgacaacgat
    23101 ggtaaaagca aacaacgact tgatggaaca acaaaaacaa gataaaaaag ctgtagaaga
    23161 caaaaaagct gaaaacgatg ctaaactaaa agaattagct gaaaaccaag ctgctttgga
    23221 atctcaaaaa ggcgatcttt tatctaaaca agctgatttg aacgtactga aaacttcatt
    23281 agctgctgaa caagcaactg ctgaagacaa gaaagctgac ttgaaccgtc aaaaagcaga
    23341 agctgaagca gagcaagctc gtattcgtga acaacaacgt ctagcagaac aagctcgtca
    23401 acaagctgca caagaaaaag ctgaaaaaga agcacgcgaa caagcagagg ctgaagcaca
    23461 agctactcaa gcttcatcta ctgcacaaag ctcagcaaca gaagaatcat cagctacaca
    23521 atcttcaatg actgaagaat catcatcagc tacacaatct tcagcaacag aagaatcaac
    23581 aacacctgaa tcttcaacag aagaa
//
LOCUS       DAZKMB010000035        23020 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000035 DAZKMB010000000
VERSION     DAZKMB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.035"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            242..406
                     /locus_tag="ACG8V3_001214"
     CDS             242..406
                     /locus_tag="ACG8V3_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299764.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            403..633
                     /locus_tag="ACG8V3_001215"
     CDS             403..633
                     /locus_tag="ACG8V3_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299765.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            818..1363
                     /locus_tag="ACG8V3_001216"
     CDS             818..1363
                     /locus_tag="ACG8V3_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0299766.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            1460..1681
                     /locus_tag="ACG8V3_001217"
     CDS             1460..1681
                     /locus_tag="ACG8V3_001217"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0299767.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1838..2002)
                     /locus_tag="ACG8V3_001218"
     CDS             complement(1838..2002)
                     /locus_tag="ACG8V3_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFQ0299768.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            2104..2625
                     /locus_tag="ACG8V3_001219"
     CDS             2104..2625
                     /locus_tag="ACG8V3_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0299769.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            2612..3052
                     /locus_tag="ACG8V3_001220"
                     /pseudo
     CDS             2612..3052
                     /locus_tag="ACG8V3_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3092..3250)
                     /locus_tag="ACG8V3_001221"
     CDS             complement(3092..3250)
                     /locus_tag="ACG8V3_001221"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFQ0299770.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            3343..4104
                     /locus_tag="ACG8V3_001222"
     CDS             3343..4104
                     /locus_tag="ACG8V3_001222"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0299771.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            4291..5643
                     /locus_tag="ACG8V3_001223"
                     /pseudo
     CDS             4291..5643
                     /locus_tag="ACG8V3_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            5659..6879
                     /locus_tag="ACG8V3_001224"
     CDS             5659..6879
                     /locus_tag="ACG8V3_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0299772.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            6914..7591
                     /locus_tag="ACG8V3_001225"
     CDS             6914..7591
                     /locus_tag="ACG8V3_001225"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0299773.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            7588..8781
                     /locus_tag="ACG8V3_001226"
     CDS             7588..8781
                     /locus_tag="ACG8V3_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0299774.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            8796..9122
                     /locus_tag="ACG8V3_001227"
     CDS             8796..9122
                     /locus_tag="ACG8V3_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0299775.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            9126..9461
                     /locus_tag="ACG8V3_001228"
     CDS             9126..9461
                     /locus_tag="ACG8V3_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0299776.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            9458..9853
                     /locus_tag="ACG8V3_001229"
     CDS             9458..9853
                     /locus_tag="ACG8V3_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0299777.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9850..10221
                     /locus_tag="ACG8V3_001230"
     CDS             9850..10221
                     /locus_tag="ACG8V3_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299778.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            10237..10797
                     /locus_tag="ACG8V3_001231"
     CDS             10237..10797
                     /locus_tag="ACG8V3_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0299779.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            10922..11233
                     /locus_tag="ACG8V3_001232"
     CDS             10922..11233
                     /locus_tag="ACG8V3_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299780.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            11257..11448
                     /locus_tag="ACG8V3_001233"
     CDS             11257..11448
                     /locus_tag="ACG8V3_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299781.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            11448..15296
                     /locus_tag="ACG8V3_001234"
     CDS             11448..15296
                     /locus_tag="ACG8V3_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0299782.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            15297..16169
                     /locus_tag="ACG8V3_001235"
     CDS             15297..16169
                     /locus_tag="ACG8V3_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFQ0299783.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            16166..17215
                     /locus_tag="ACG8V3_001236"
     CDS             16166..17215
                     /locus_tag="ACG8V3_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299784.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            17219..18043
                     /locus_tag="ACG8V3_001237"
     CDS             17219..18043
                     /locus_tag="ACG8V3_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299785.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            18061..20121
                     /locus_tag="ACG8V3_001238"
     CDS             18061..20121
                     /locus_tag="ACG8V3_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0299786.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            20138..20287
                     /locus_tag="ACG8V3_001239"
     CDS             20138..20287
                     /locus_tag="ACG8V3_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299787.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            20284..20730
                     /locus_tag="ACG8V3_001240"
     CDS             20284..20730
                     /locus_tag="ACG8V3_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299788.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20732..20869
                     /locus_tag="ACG8V3_001241"
     CDS             20732..20869
                     /locus_tag="ACG8V3_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0299789.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            20904..21146
                     /locus_tag="ACG8V3_001242"
     CDS             20904..21146
                     /locus_tag="ACG8V3_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFQ0299790.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            21162..21359
                     /locus_tag="ACG8V3_001243"
     CDS             21162..21359
                     /locus_tag="ACG8V3_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0299791.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            21370..22386
                     /locus_tag="ACG8V3_001244"
     CDS             21370..22386
                     /locus_tag="ACG8V3_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0299792.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            22501..>23020
                     /locus_tag="ACG8V3_001245"
     CDS             22501..>23020
                     /locus_tag="ACG8V3_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299793.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGE"
BASE COUNT         8004 a         3909 c         5101 g         6006 t
ORIGIN      
        1 aaatttttat attgtcactg tggcggaaag ggtagacgct aagcatgtgt gctaggtcaa
       61 tgcttcggca actatgcaat gttcgattca ttgccagtga ctttggcaac cgaggcatcg
      121 gcggtttaaa aatatagggg tgcgcaattt cgtacgcgtt ttgtgcatcg tgtaggttgc
      181 tattacatat tagatcactc gttgagtggt ctttttattt tacataaagg aggtaacaac
      241 gatgtataga ccacaatact tagaacagaa gtatgaagta atcactgtgc aaaacggtaa
      301 cggtgagata gtacgaaagt atagaagacc aataaagagc gatacatata aacgaaagga
      361 aaacaatgaa gttatttctt ttcgcagaag gagaaaagcc aaatgagaaa ctactggtat
      421 gtatcgctaa ctaatgaata tcctcgaacc attgatgatt gttcagtgcg tgttgtgcgt
      481 tctgtacaaa tcaaagggaa gtactctatt gtcgaaatgc taagagaagc tacaccaaac
      541 gaagtggata aatgcaagct gatatattgc ggtcatggct attggaaaga cgagtatatc
      601 caacaaaaca ttgagaggtg gatagataga tgagttacct cgaacatttg aaacgttgct
      661 acatgcattc taagaataaa cttcctgaca gctacactgt agatgatgtt gctatacatg
      721 tattgaagac tgagagccat agcagtcctg atggtagcag taaggagcag acgctcgcgt
      781 ggttcaagtt ctttaagtgg attaaggagg aagagtaatg cacatgcaca taggagaaaa
      841 gatatataaa ttgagacggc ttaataacga aacaatgaac gacttagtta aacaaactgg
      901 agtgtcaaaa tcatggttat ctgatattga gtctggaaaa aagcaacgcg ttgattttaa
      961 taagatatgt gcaatagcca aacattataa agttagctta gaatactttc gggatgatta
     1021 tttggatagt gatgaacacg aggatattat tcctttgaca ggcgaaacaa gagttaaatc
     1081 agtgaaatcg cttgaacaaa gcctaaagta tctgaataac aaacaagaac ctaaccttct
     1141 agagatcaaa ctaaaagata ctgactcagt accagaggtt tggtacaagg gtgagaggtt
     1201 ggatgaattg cctaaaggat tagtagatgt ctcgtatcat tggaagactg atgattttac
     1261 taatgatgat agaggagcga acgacatcac gattcaatac ttttctagct ttaatgataa
     1321 gtatccagat aagaaaacaa tcggacacaa gagagatatg taaatgaaag aagttagacc
     1381 tagagacgaa atagacaaac tatacaagac caaacgatgg cgagacctaa ggcaagtagt
     1441 aatagctagg gacttcggca tgtgccaaga gtgcaagcgt cgagggcgga acacaagggg
     1501 cacgatcatc catcacatag tcgaggcgag ggaagacctg tcactgttct ggtccgtaga
     1561 taaccttgaa tgtatctgtg tagcttgtca caacagagag catccagaga gatcaggcgg
     1621 gaagaagaaa ccaaaaccta aatcacatat cgttaaaatg tattcaactc ctgaaagata
     1681 agtttgcagc gaaatgaagg tagcccccct actctaaaag attaaagagt aaggcttgag
     1741 aagaacggtg ctgtccttcc ttcgtaaaaa gaccgctttt caagtttttt ggagaaaaag
     1801 gaaaaagccg accaatttaa gccggctttg gacgaagcta tttcttagtc catttgtttc
     1861 ctttttgaga agtagggggt aatcggtcgc ctggatcaat ttttacttct tgtccgcctt
     1921 ggacatttcc accacgagga cccacttctt tgtaggttcc ttttggtttg ttgtcttcgc
     1981 cgggtttata gagttctccc ataggaatcc ctccttaaca aatttcggca cagcactgcc
     2041 gataacctaa ttataaggat tgtgataacg atttcaatct atcttttgaa agaaggtgat
     2101 attatgccgc aaccagcgaa gagtgcaaaa ttacaattat taaacggaaa cccaaataag
     2161 aagaataccg aagaactccg caagcgagcg gccgcagaag acaaattaaa aatggctact
     2221 gacaaaatca aaccgccgtt atggctagat tcgctaggaa aggatacctt tgagtttatc
     2281 gccgatgaat tgctgtctgt ggatttaatc agtaatccgg acgtccatac aatggctcta
     2341 tactccaatt ggtattcgca atacgtttct ttagaaaaac agcttcgaaa actacaacga
     2401 gagtacaagt tgaactatgc gcttgcgaaa aaggaggcag aggcgagagg tgagccgttt
     2461 aatgaaccta atgaattaat tggtaatccg ctctctcggc agatggatac agcgtctcgg
     2521 aatctccgtt cttttggcgc tgatttagga ctatcaccag cagccagagc taagttagct
     2581 attaagatgg ctgatgatgg tggtgatgac gatgacgact tctaatattt tggatatgtc
     2641 ttacacagaa cgtgtggact attggcaaag ctatcttgag gagcaagctt cttggggtgg
     2701 ctttttaaaa tgtccatatc cggaattgtt aactacttgg tatgcggaac gattaatcga
     2761 tggaagcata ccagccagca aagaaaatat tcaagctgct aaacggcata tgcgtgattt
     2821 gcagcgccaa ggaacagatg attttccttg gatctttgac gaagaaaaag gtcaccggcc
     2881 tattagatat atcgaaaaaa aatgtaaacc aactgaaggc gactttggtt cgtttgtttt
     2941 gcaaccttgg cagcatttca taattggatc catgtacgga tgggtacatc gtgatacagg
     3001 agagcgtcgc ttccgcgagg ctcttatttt tgttggacgt aaaaacggta agttttgccg
     3061 tttaaaaatc gggcaaaatc ggtgaaaacc tttatttttg gctttctttt agtaataatt
     3121 cgatagcttt atcaagcaat ttagacatag gaaccatagt ttcttccgac atttttttta
     3181 atccttcgta taaatcttta tcaattgcgt tagaaattct aattctgttt tttagtccgt
     3241 atttgtccat gtgtgacact cctttttatt ttactataac atccgattcc accgcttgca
     3301 actaccatca attgatggta gtatataaaa gaggtgagta gattgaaaaa atacattatt
     3361 tacaaacact taaataaaat aaatggcaaa atttatatcg gtgtcacaaa tgatattggc
     3421 agaaggtgga ggagcggtgg gattgaatat aaaccaccaa agaacgaaac ccaacaccac
     3481 agaagttttt ggaatgcaat acaaaaatac gggtgggaaa attttgatca tttgattatt
     3541 gaagaagact taacgatgaa agaagctttt gagaaagaaa agttttatat tgaactttat
     3601 gactcaacta ataagaaaaa aggttataac atcgcaaaag gcggaaatgg cggaataata
     3661 tataaagttc accccaaagg aatgctaggg aaaaaacaat ctaaagaatt ttcttcaaat
     3721 cacagtaaat gggcaaagaa ccataagaat aattgtatga caaacggtga tgttgtatgg
     3781 ggtgttactc acgaacaccc aaaaggcatg ttgggaaagc accacagcaa agaatcaatt
     3841 atgaaaaaga aagcatatag tggtgaaaat gctgtaacct caaaaccaat tgtcgcaatt
     3901 gaacgggatg gaactaaaag agaatttcac agcgctaagc tttgcatggc ttactattcg
     3961 attagtacat gtgtttttta ccgactttta aaagatggcg ccccttatgt gataaatcct
     4021 aaagcgaact acagaaataa agaaaaaata ttagctatcg aaggaattat gttcaaacat
     4081 aaagaagata ccgaggtaag ttagtagatt gcgaaaggtt actaactacc gtaacgcgta
     4141 ggagttgaat aaatataatg ctcccaagag tgtccgacag cagataattt gctgaaaatg
     4201 tacgctaaac tgggccagaa ctgactgacc gatgaaaatg agggtgacct ccagagtgcg
     4261 agataaaaag ctcgcagata ataacaatcg aaaacatcac taatttcagg cctttccaca
     4321 tacatggtcg cttatgatga tgaacaaggc gccaacgttt acgtattggc aaatgctcgt
     4381 gatcaagcaa gcttgttgtt tgataaggcc gcagaaatgg tcaaacaatc gccggcgctc
     4441 tttaagaaat ttggtaagcc taaacgatca agtattaatt atgctcccgc cttttctaaa
     4501 atggaaccac gcgcctcaga tagccggaaa ttggatgggc taaacactca ctttggtatt
     4561 tttgacgaga tccacgagtt tacgaattac aatctgatca acgttatcaa gaaatcaaga
     4621 ggaaccagaa aacagcctct gatagtttat atcacaactg ctggatatgt attagatggt
     4681 ccgttgatgt cttattttga gcaaggtgtg gactgtttgg aacatttgga agatgacatc
     4741 gatgaacgga ctttctatta tctggcaaaa cttgacagtg cggaagaggc tgatgaccca
     4801 agattatgga tcaaagccaa tccgaatatt tgtctaatga attttgttgg catgctagat
     4861 gactatgtta aggataaaaa agatccaaaa gaatatgctg actggattac caagcaattt
     4921 aacttgtttt ccgatatcga tgagctgtca tttgtcgata tgcctaccat taaacgaaac
     4981 aataaaacca tcgatattga aacgctcaaa ggtaagaagt gtgtcggtgg ttttgacttg
     5041 tccgaaacgg aagactttac cgcagccgtt ttagaatttc cgcttgaaac aggcgaggta
     5101 ttcattttgc aacacacatg gatcccacaa gctagatttg atcgagataa caatcaagag
     5161 cgtatcaaag cgtgggagaa ggtgggagat ctaacgatta ttcctggtga ttacgtcaat
     5221 tatgaatacg tcttaaattg gtttgtagaa aattcgaaaa tctatgacat tgtaaaaatc
     5281 aattatgaca aggccaaggc gctacgatta aataaagaac tagaaaatgc aggatttgaa
     5341 accaacgaga ttcggcaagg gtttctatca ttaggtgggc caatgcaaaa cttcaaggaa
     5401 atgctattgg acggtaaggt gattttcaac aattccaagc tttaccgatg gtatctatcc
     5461 aacgtcaagc tggtgatgga tcgcaactca aactggatgc cgtctaagca gtccaagagt
     5521 agaaaaatag atggttttgc agcaagtttg aacagccacg ccgaagtgtt gaatatgttg
     5581 gttaatcctg tcggaaccgg gaaagtaacc tattactcga tttccgattt aatgaatatg
     5641 taagaaaggt gtggaggaat gagtatttta gatcgtttgc gttcttttgg ccgagcgaag
     5701 ccgaaagcga gcaaacaaga gtattttttg aatgacccgg gattgatacc gtatttagtc
     5761 ggaaaagatg aaatatcaga agggattttt tccgtaatta gccgtgtatc gaacgttttt
     5821 gcgtctctcc ctctcaaaat gatagatgtg gagtttggcc aaccggacga ctgtcctgca
     5881 tacaacttgt tgagcgaagg ccctcgatat tttacaaagt ttgatttttt ccgggacgtg
     5941 gaagttttga gaaactacca agggaatgcg tatgtgcaga ttttccgaaa tatcaatgga
     6001 gaagtagcag atatggcgtt agtaaaacct ggtgcttgcc atccagtgat tgatatggat
     6061 agcggggagc tttactacca agtaactgcg actgacaaag gcagttacaa gcaagttatc
     6121 tatgtacatt acatggaaat gcttcacttt aaacaaccga ggtttggcgg cttggaaggt
     6181 acagacccca caaaagtatt aacgaatacc ctcggatatg atcgagaagt ccgaaaaatc
     6241 tctttaagtc agcttaaagg aagtaatgaa gggctaaaag ttaagtttgc tagcaatatg
     6301 gatgaagaag ctaaaaaagc tacagttaaa aacattgctg atttttatcg acaaaacggt
     6361 ggactacttg tggaagaaaa cggtgtagaa atcgaacgtt tacaacgaga gctggtagac
     6421 agcaagcttt tagatactga taaagtatct cgctccagaa tcgctatggt ctacaatgtg
     6481 ccggagcatt tcatcgggaa taaccagtcg agttactcct ctcaggaaca gctcaatatg
     6541 gaatttttga cgtacaatct agtaccgacc gttaatcaat atgaagcgga actaaataag
     6601 aaaacactat cgagaaccga aaaagcaaaa ggttatcgat ataagtttaa tgtcgcaagt
     6661 ttgctaagag ctgacactca ggcaagggga caattctatc aaatcatgcg acgaggcgga
     6721 gcatactcag cgaatgatgt ccgaagattt gaggacttgc aaccgataaa taaaaccggt
     6781 atggatgatt accatatttc cggagaccta tatccaatcg atatggatcc aacattaaga
     6841 aaaacaacct cgtctaaaag cgtagccgaa aacggttagg tttttttagt ttgcaccgaa
     6901 gggaggtgga aaaatgaaaa aagtgacgtt aagcggcgat gtcgtggata acgataccgc
     6961 gtggctttat gactggtttg ggatcaattg tatctcacca gggaaaattt ctgccgctct
     7021 tacagaagca gcgggggatg aagtagaact tgatatctca tcgaacggtg gggatgtcct
     7081 agcggcaagc gaaatatata ccgctatccg tgcctatccg ggaaaggtat ccggaaatgt
     7141 tgtgagtatt gcagcaagcg ctgcaagtgt aatcgcttgc gcttgcgaac cgcttagaat
     7201 ctcacctacg gcacacatca tgattcataa cgcatgggtg accactagtg gcaacgctga
     7261 ggaattaaaa gccaatgcag aaatgttaag cagtgtggat gagtctattg ttaatgctta
     7321 cgagatcaaa acaggactcg atcggaaaaa acttgctgat ttaatggcga aagatacttg
     7381 gttaaatgct caaacagcag tagcagaagg gtttgcagat gaaattatgt ttgcagaagc
     7441 accagtaacg gtactcaatg cctctcaacc ggttattcca aaaaacgcag taactaagtt
     7501 gaaaaattta atactcaaag cggaagcacc gcaagaagaa acactcttac agaaaaaact
     7561 aaaagcctta aatggaggga aaaacgaatg aatttagaac aattaaaaaa tgcgtgggtc
     7621 gaggcgggaa gtaaagtctc tgacttaaat gcacaactca atgcagcatt ggttgacgat
     7681 gaaaaaacag aagaagatgt agtaagtttg caagcacaag taaaagcagc acgggctaaa
     7741 cgggacggat tgaaagagca agtggcaaat atggaagccg aacaagtctt aaacgtcaaa
     7801 aaagaaccat tagataaaaa agatgaaaac ttgaaaaaca agtttatcaa agactttaaa
     7861 gcgatggtca atggtgatcc tgctattatg gctactttga catctaatac ggatgaatct
     7921 ggtaatgcta tcggattgac tattcctgta gatgtgcaaa cgactattca tactttggtt
     7981 cgtcggtttg actctttgca agaatacgta aacgttgaaa aagtgaccac taccagcggt
     8041 tctcgcgttt atgaaaaatg gtctgatatt acaccgttga ctgctttgga tactgaagac
     8101 ggtgaaatcc cagcaaatga tgatcctgca ctttacttga tcaaatactt gatcaaacgc
     8161 tatgcaggta tttccacagt aactaacagc ttgctaaaag atactgccga aaacattttg
     8221 gcatggttgt ctaaatggat cgcgaaaaaa gtagttgtta ctcgcaatac aaaaatcttg
     8281 gcagctattg atggaatcaa agcggcacaa aagaaagatg ttacagatgt tgatggaatt
     8341 aaagatatcg taaacgtcca acttgatcca gctatcgaag ctacatctat gtttattaca
     8401 aaccaagatg gcttcaatgt tttagataaa gtgaaacgtg ctgatggatc ttacttgtta
     8461 caaaaagacg tagcttctgc aactggatat accttcttag gtaaaccaat caagaaaatt
     8521 gcttctcgtt tcttgccaaa caaaggtacg caagctactc ctaaatatcc actgtacatt
     8581 ggtgatctga aagaagccgt tacattgtat gatcgcgaaa acatgagctt gctgacaacg
     8641 aatattggtg gtggagcttt tgaaacagac accactaaag tacgcgtcat cgatcgcttc
     8701 gatgtgcaac tagttgatga tgaagcggtt gttttggcta cttttacaac tattgcgaac
     8761 gagacaccgg cgaaagttta aggagctgat tccttatgat tcttgatcct aaaatggatt
     8821 taggcgaaat caaaaacgca ctaaagattg ataccgatga tgacgatgtg gaagtaagcc
     8881 gtgcggcaca agctgcaatt gcatacatta gaggggctat cgggaatgat aagccctctt
     8941 tttataagca agaaaatgaa acggttgatc tgattaattt agctatcctg caattagcag
     9001 atcattatta caacgctcga tcagcaaccg tgagtgggaa cttgcgagag tacgatttag
     9061 gttttacaag cctaatcttg caactcaaag caagttattt gctttttgtg gaggaggagt
     9121 agcgtatgcc ccttatccaa acaggaaatt taaatcaacg catcaagttt gtccgagata
     9181 cgacggttaa ggatgaggac gggcaagttg tcccgacttc tacaaccatt cttacttgct
     9241 gggcaagtgt gcagacacaa cgcctgaacg atattaagac gtcgattggt acggctttgg
     9301 aaggaacact gacgttcatt atccgctacc aacaaaaatc agagctgacc aatgatatga
     9361 aagtgcgttg gaatggaaaa acgtttgaaa tcattacgat tacgaaaggc gagtttgcga
     9421 aggacttcac gacaatcatt gcaaaagagg tttcaaaatg agtgtagaag tcgatgcaac
     9481 cgaagtgtac aaagcgctta gggaagtaaa agcaaacgtt caacgagtgg aaagcccagc
     9541 acttagaaaa gctggggagt acgctcaaga agagttacga caaaacacac cttactggga
     9601 tggaacgaag tcaaacggta aacgtggttc gtatatgcaa gaacatgcta agaaccatgt
     9661 ggttacaagc tcgataaaaa acggattggt agaagtcggc tatgacaaag atgtttcttg
     9721 gcggatgcac tttatcgagt tcggaacaat caaacaacgt ccaaaaggtt tcgtacaaaa
     9781 aacacaaaag caaatcgaaa aacaagtaac acaaatcatt gctgacgaag taaaaaggag
     9841 gctaggactt tgaaaacggc agtatcacaa gtctattcaa ttctgaatag caatgaaaaa
     9901 acaaagaaca ttgattttta caccaatagt gttccggaat cagctcaaac agtacctagc
     9961 cttccagttg gcagaattac agagatatcc ggcaactatg aagattttgc aagcaacaat
    10021 cctttgacca ttcaatttaa cgtacaggta gatgtatggg tgtcaaccat gaaagaggtt
    10081 gatgcctttt attttgccct tgatgaggtt atgaggggga atggttggca atgcgcatac
    10141 acggaacaaa cagatgacga ggacttggaa ggtgcaaagc ggattatcaa acgatatgta
    10201 gcaaatattt cactaaatta aaaggagaga aaatagatgg caacagtagg attcgagagc
    10261 gtcatttttg gcgtaaaaac aggtgcaggc ggcactctaa aagaattagt agcagataag
    10321 tcgaaaggcg gagcgatcga agctaaaatt actggattag gcgcaacttc taacacaaca
    10381 tacgcttcaa acgtaccgtt cttcattgca agtaaagggg tttcgtcgcc aaaagttacg
    10441 cttgacgtgg cagacttaat ggataacggc atttacagcg aaatcattgg tgctaaaacc
    10501 gtggatggtg caaatgtaat tggttcagaa actgaagcgc cttacgtgtc ggtagtcatg
    10561 gttacagcga acaaagaagg aaaacgcttg ttcatgggat tgacaaaagg aaaattcagt
    10621 catccagata tcgacatgaa aacagctgaa gacaaagggg tagaattgca aaccgattcc
    10681 atcgaagggg aattcatttc tgatgaacgt ggctatgtat acttaacagc cgtagaatca
    10741 gaaggaatga ccttacaaaa attcaaggac ttggtaaata acaaagaggg ggagtagtta
    10801 accctgcatc tacaccaacg acagatacag ggacaccaaa agaaccagaa ccaaaaattg
    10861 atacacaagg ttagccattt ttggctagcc ttattttttg taaaaacaag gaggaaaaca
    10921 aatgattgaa ttgcaattga aacttgacgg aaagaaaaaa acattcaaac aacaagatat
    10981 ttccgcacgt gcaatgcgtg agtgtatcaa attttacgag aaagcggaaa aagcagacct
    11041 aactgattta gaagcgattg attcaatgat tgcaattaca gcagatattt tccaagatcc
    11101 agcagttaca tttgatgcta ttttagacgg tttgactgcg agcgagttag taccggcatt
    11161 agaaagtgtt tttgaacaaa taaatgaact gggaaacaat gaaaaaaagc agacggcgag
    11221 caaaaagaaa taagtttttc tgaagctagg aaagcaatgg atcaaatcta caaagattta
    11281 atcgaagcag gttggacgat gagagatgtg gacgaagccg actatcatta tttgttacac
    11341 ctttttggag aagtggagag tggcgaagaa tatgtagatg gtgctgattt catcaaacaa
    11401 tttttatcgg ctgaagactt agtaaaactt gaggaaggag gtaaataatg gcaggaaaag
    11461 gacaaccggc aggaaatatc aagctaggga ttagtttaga tagcactagt tttggtaaca
    11521 cgctggacga aatcaatgcg aaagtcaaac aagctgagtc gaatatgcgt gccaatctaa
    11581 aggcttatga ttcagcagga cgttcatacg aagcacttag tcaaaagacg aaagacttgt
    11641 ctacggttat ggaagggcaa aacgccaaag taagagaatt aacaaagcgc cgtgatgaag
    11701 cgattagcaa gtatggcgag gaatcgaaac aagttgctaa ccttaacaca cagataaaca
    11761 atgctaccgc aaaatataat gcttacagtc gccagttgaa cgacacaaaa aaagaattgg
    11821 tgtattccaa aacagccgtc aatgatttat ctaatgaaat caaagaaaat gaacgacaaa
    11881 tgaacgccga agtaaaagcg ttgaaagccg ctggtgatga atctggtgct tttgaagcaa
    11941 aacaaaaagg gctagccaaa caaacggaat tatccgagaa agctatcgaa gaacagcgca
    12001 aagttgtgaa actgatggct gatgagtttg gcgattcagc aaatgaaacc gaagatgcaa
    12061 aaagggcatt agaaaagtta gaacgacaaa gccaaatatc tagcaggcaa ttagaagcac
    12121 tcaaaagctc cagcgatcaa tcaggaaaag aaatagaaga ttttggcgac aagtccacaa
    12181 ggtcagctag gaaactggac ggactaaaag acaaattaag ctcgctaaaa agcgcatttt
    12241 cgtttggtgc agttgctgga ttagcgcata acgctattag cagtgtagta agtggcgtgc
    12301 aaggtttggt tggcgaagca gtaaacgcat cggattcatt gatgaagttt tccaaaacca
    12361 tggagtttgc taactttggg aagtcacaga tagaaagctc gaaaaaagaa atgaaagact
    12421 acgccgataa gacggtttat ggtttagaag aaattctgaa cacaaccgca caattggcat
    12481 ctaatgggat tcctaactat acagaactaa ccaaggcggc aggtaacttg aatgccgttg
    12541 caggcggttc tagtgataca ttcaaatccg ttgccatgat gctaacgcag acggcaggag
    12601 ctgggaaact aacaactgaa aactggaatc aattagcaga tgcgataccg ggtgcttcag
    12661 gattgttaca agacgctatg ttgaaaaacg gagcttatac aggaaacttc cgtgatgcaa
    12721 tggcgcaagg tcaaatcact tccgacgagt tcaaccaagc aattgtacag ttaggtatga
    12781 atgacggagc agttaaggca gccacttcta cagacacatt gagcggttct tgggagcaga
    12841 tgaaatccac tgtaataaat gggctacaaa gtattataga aaaaataggc gttgaaaata
    12901 tcactggttt catcaacaga gtaacaaaag ggattgaaaa ttctattcct aaaattactc
    12961 aatttatagg ttggttgaga gatattggaa cgtggatcgt tgaaaataga gagccactaa
    13021 catggattgt cggaatcata ggcggaatta cattagcagt aaaagcattg aacgtagcaa
    13081 gtatgttgct ggcaattact ggcggaacat tggcagcccc ttttgtggcg attggtgtag
    13141 cattaggcgc actagcaggt gctttggtga tagcttatac aaaatctgaa acatttagaa
    13201 atatagtcaa tgcggctttt acagctgtga aaaacgtagt tatgagcgtt atcaataatt
    13261 tggtggaata ctacaaaatg ttgtggagcg tgttgcagtg gctttgggaa aaaataaaag
    13321 aatgggcttc atggattggc aataaattca ttgaaatgaa gaacagcgtt gtgaatacgg
    13381 ttcagaacct atggaatggc gtgaaaaact tcttcagcaa tggcgttgga gatacgtgga
    13441 ataaggtagt cggttgggta aaaaacattt tcaacaaagc aactgaattg aagaacaacg
    13501 tttctgatgt aatcggtaac ctgtggaatg gtatcaaaga cacattccgt agaggtatcg
    13561 atacggtatt caattggttt tcagaactac caacgaagat gaagaatgcc attattggcg
    13621 gtaaaaacgc cattgttgat gcgttcaaaa gtattttcaa cgcagcactt aaagcgatag
    13681 gtaaaccagt taacgcaatc atccatggag cttcatgggt actagaaaaa ttgggcgcag
    13741 aacctctaca agaatgggat gtaccacaat acgctacagg tacaccagca ggtgggcacc
    13801 caatcaatgg tccaatgatg gttaatgatg gacgtggagc agaaacagtt atcacaccag
    13861 atggtagagc cttcattcct aaaggacgca atgttgttct aaacgcacca aaaggaacgc
    13921 atgtcttgac agcagaagag accgcgcaac ttcaaggttc taaagctcct aagtatcgtt
    13981 acaaaaaagg tactaacttc tttggtaaca tgtgggatag tgtgaaaaag gttgctggta
    14041 atgtaggtca cacaattaaa aacgtagtag gtgacgtgtg ggactttatt tcagacccgg
    14101 gagcgttagc tagaaaagta cttggggggt tagatgtatt aggtggattg acaaagtatc
    14161 cattagaagt aggtaaaggc atcctatcta aagcaacaag tgcactgact gaaaaaatca
    14221 ctgggttgtt ctcatctggt aacttagata cctccgtagg aacaaatggc gtctataaat
    14281 atttggctga tgttgctaag tctgtgatga agaaattccc aggatttgtg gcaactagtg
    14341 ggtatagacc aggtgaccct tattcacatg gtaaacgtaa tgccattgat attgcattac
    14401 caggcgtcac aggaggctca cctagataca cagaagcagc aaactacgct tttgacaaat
    14461 tcgcttccaa aattggttac gtaatcacta atgggaaagt tcgtgaccgt tcaggacaat
    14521 caggtcaacc agcaactggt gcatgggagc catggcccga tggagatcac tatgatcatg
    14581 tgcatttaaa cggtgtgaaa gacccacaaa acactcaaat ttcaggagat agcgtgggag
    14641 gcagtggggt agaaagatgg cgcaatgtag caattagagc gttgaaaatg accggtcaat
    14701 acagtactgc aaacttaaat gcattactaa atcaaatgcg tacagagtca aatggtaatc
    14761 ctaatgcagt taacaattgg gatattaacg ccaaaaatgg aacaccatca aaagggttgc
    14821 tccaagtgat tgacccaaca ttcagacagt atgcaatgcc aggattcaac agcaatattt
    14881 ttgacccact atctaacatc ttagcttcaa tcagatacgc actatcaaga tatggctcac
    14941 taacaaatgc ctatcgtgga gttggttacg caaacggtgg aattgtaaac caacatcaaa
    15001 ttgcggaaat cgcagaagga aacaagccag aaattattat tccgttagat aaggctaaac
    15061 gatcaagagc gatgcaatta ttgtctatcg caatggacaa actaggagtt tctcctaaaa
    15121 cttatggaaa tgcgacaact gtttccagtg attacgaaat cctagaagcc attgaaaaac
    15181 aggcggcaac aacgaatcag ctgttatcgt tattgctggc atttttcaaa ggaaatagcc
    15241 gaactgatag agatttagct ttagatattc agaagatctt ggttaggagg atgtagatgc
    15301 gaactgtatt actgagaaat agaataaacg aagaaattga tttgtccaca gaagactatt
    15361 ttgctactgg gttgagtaat atggggtttg aagtaaaaaa agaacatgtg ggacagtggg
    15421 ggaatttcag agaaagcagt gagagcgttg aaatatctga atttcagtca tctgtaatca
    15481 tttctgtgca tgggtttcga gaaaaagaac tgtacaattc acttgtgcag ttcctatcgg
    15541 aaggtccgtt tgaactggaa tttgcttttg acggcgaaac aatggtaaga agatgcagtc
    15601 taaaatcttt aagtaagact gaaatcgatc cgaaaacatc gttactaaca gacactttag
    15661 aactatattt cacatcaaac tggtattccg taaaacgcga aaaattaatt cagcgaccga
    15721 atgtagtgaa aacacgtggt aaggtttttc cgtataaaag atcttatatc tacactcaaa
    15781 acttgtggga gaaaaaaggt gtattcaaat tcaataataa ttctgtatac ctaacgaata
    15841 gcaaggaacg gatgtccccg ctaaaaattc gtgtgattgg gaagtgttca aatccgtatt
    15901 gggaagtaat ccaaaactca caaatcatcg caacggatgg atacttcata gatatgactg
    15961 aaacacagac tctagaagta tcaagccttt ttgaagatac aacggcgatt ttaaaagata
    16021 ttgcaggggt agaatcttct gtctaccaac aacaggatta tacaaaaact aattttgttc
    16081 aggccccaac tggagaattc agcattgtgt ttcatgttgg gggggcagac gtagagattg
    16141 agttatacga ggagcgtgat ctgttttgat tttagcagtc acgctttttc atcgtgattt
    16201 aatgctttat aacgaacatt tattctctaa cagttttgaa tttggtgtgg acgagatcaa
    16261 cgaagaggct agtagcttta cgatggataa gtacgttcct gtaaaaacag gcgattttct
    16321 actagcaaaa tatattccta gtggaaaatt tgcatatttt ggtgtaatta cgtcgcagga
    16381 agacgaaaaa atcagttgta aaagcttact tagtttagct gatagtgaga taccgactgc
    16441 acgtgtgtca ggagataact acgaagagca tatccggcgg ttgattgaat actatttgct
    16501 gaatgatcca acgaaacaac taaaagatat tttagacgtc aaagcagaaa gtgcgacctc
    16561 tcattcgtat caagctaccg atacgaataa acacaagtta agtgcgtata ttctcaatgg
    16621 tttcaaaaaa tacaacgtaa aatggtattt caagggaatt caaaacagaa aaatttatac
    16681 aggcatacgt gctgtaaatg aatcaattta cattaaagac aattcttctg aatttagcga
    16741 ttgggatgtg tttgttcagg cgccgggcgc tggaaacgag aacaagctat taatcgttga
    16801 taaagcaatg aaagatatag agaaaccgat aatactgtca acatggtatt tggacgaaga
    16861 aaacaatttg acacaagatg gatcgaaaga aaatatcacg aaaccaactg tcaatttggt
    16921 taatatctac gatcaaaccg cagaagataa agcatcctac gaagatgtgg caaaatcaga
    16981 gttgaaaggc aatacgtatt cgcatgaaat caaagtgaac gttgtaagaa atgcaaaaaa
    17041 tttgaatgtc gaaacgattg aaacagggat gtttgccacg atttcttata aaggaaagat
    17101 atacaagtcg gttttgacag cttggcgaat atcaagtgat aaggagtttg tggaattgac
    17161 tttcggaaac ataagaagtc gttttatgga ttattttgaa gataatgggg gataaaaaat
    17221 ggttagcaat gtggatggat atcaatttga aaacgtgaaa gtaagcgcag aaaatgatgc
    17281 tagactttat cacgttttat ataatcggaa aaatcaggtt attgatggtt acgatcagtc
    17341 tatgaattta tcttcaagcg gattaacagt aaaagttgct gcaggagcag cgattattca
    17401 aggtcgtatg gtcgttgttc gacaagaaga aagtataaca gttccagcaa actcaagtgg
    17461 ttatatagca ttaacagtgg atttgacaca agaagttata cctggatcta ttcttccaga
    17521 atcggaagaa tatgaatgga ctaataatca agtcaagcta gaatttataa caaaagttat
    17581 aaaaggtaat ttaaacaatg gtgataaggt ctataattta ccactatgct cagttaattc
    17641 tactggatca actgtttcaa tctcaaagat atcggatagt tacgagctga ctctctctaa
    17701 aggagaaatt ttgtggaggg ggactgcgtt aatgcatgat actcaaactg tccaaccttc
    17761 aaaaaaaatt tggcagacag tttcaggttt tttgttagta tggctcccat acgaaaacgg
    17821 gcaagcaatt gaggatagat atgtaactac gcctttttat aaggagcgtg taacttttac
    17881 taatgtttta ggagaaattg tttcaggatt tgatgactat cacaaaaagt ggtttagtaa
    17941 acgaataaac tataattcga atactaatat atttacaggt gcagcaagca atgcaagtgg
    18001 agataacgca aatatggtgc ttaggtatat agtttctttt tagttaggag gtatggaaga
    18061 gtggcaaatt tagaaattaa attatctgca aataagagac agccttatct acgtcaccgt
    18121 gttgttggta gagttggtga tggggggctc acaacaatca atgtacaact tcttgaggaa
    18181 gatgaaatta caccttttgt aattaatcta aacggtactt tgaaatttgt gggcgaagtt
    18241 tcaaacggta actataccga gggagaacca gaaataatcg attcgactaa tgggttaatt
    18301 agttacacgt tcactaagtc aaatttcagc acgagtcatc aattcaaaca agcatatttt
    18361 gaatatgtag atcctaacgg caaaaaagta acttttcaga acttcatcat agacgtgtta
    18421 gaacgagtgg atattaattc ggagcaagcg aaatactata tttcttcatt ggaaaaatta
    18481 cagagtgaaa tgcaaaccac tttcaatcag ttcattagtg ataaacaggt ccaatacgat
    18541 caaatctact cgaaatataa cgaattagta agattgataa atgaatcaga taagcaagta
    18601 aatgatcgta ttgatcaaac caatcagcaa atcggcgatc tcggcaagct gaaaaagatg
    18661 tacagtaaca gcatcgactt tgggggctat gattattcgg ggaatccgaa tttgttaagt
    18721 aagctatcat acgacttaat tgaaaatcaa aatacttcag ctggaacact ttctaagggt
    18781 gaaaactcgt ttaaatataa taagatatca gctgaagtgg aaggtggagt agagttatat
    18841 tataaacgaa gaggtatagc taactggtta ccctctaata aaacgcttgt aatgactgtt
    18901 aagcttagag ctggagcgga ctatagtcca gttgacggaa aacttatact gattagatat
    18961 aggtatgttg acagtggaac tggcaagatt gttttagact tacctattaa cagtaattcg
    19021 ataactcagg aatggaaaga gtttagtatt actggaacta ctccaacatt tagcccacaa
    19081 gcataccatc cttggataca atttagggct caagatgggg tacttgggga aatagaaatg
    19141 agctatgaca tcaaaatcga agaaggctct acagctacac catatcagcc taacttactt
    19201 gatgactctt actggctagg taaaacaccg ttgggtgaga atttgataac gaatgccaag
    19261 ttcccaatta taacacgaag caatcctatt tctggttttg atatttcaga agaattaata
    19321 ataggtgaaa cttatacggt atctttaaaa gggactaagc cagctactaa agaattccac
    19381 ttatactacg gagaagcaaa ttaccagcag ttgcagtatc aggcaacttt acttcctgtg
    19441 gaaggattaa cagatgtgtg gagtgccact tttacagcca gaaatactag tgaaacgaca
    19501 aatttactta gagtagcgtt atggcaaaaa cctaattctg caacatatga tactgttcaa
    19561 attgactggc tcaagattga aaaaggcgac acacgcacac caaatatcag tcagtttaaa
    19621 tactttggtg aaggcttgaa agacagtaac aatccgaacg actacagctg ggatgtcaca
    19681 ccagagtata ctgaaaaaag cttgaataat acggttagtc tgactgaacc gcaatccatc
    19741 gaaggattga aaaatttcga agatggtatt caatcaaaag gaaaatctgt attgacatca
    19801 gacgacaaca aatatgaagt cgtaacctta acagttacaa acgggaatac cggatcagca
    19861 aagctttatc gtgaaggaaa aaccgtcact atttattttt ttgcgttgaa tgggaaaagc
    19921 agtggtggaa atgattcaac gatactaaca attccagaag gctatcggcc accaattagt
    19981 tttgagcaac tggttggctc gatagaccgt tctactttga acagtgctca gttatctatt
    20041 ggtgcagatg gagccattaa atggcgaaga aactcaagtt atggatcgga ttataccttt
    20101 gcaattactt acacgattta gaaaggcgtg aatcgatatg aaggcagcat atagaccaat
    20161 tgaaccttac ggattcgagc aaatcattgt gaatgatgaa gaacatttac cggaagaatg
    20221 cacagaagtc gaaccaccga ttccaaattg gaaaccgaaa ttcaattact gggagggaaa
    20281 taaatgaaaa acatttggaa atatggccgt actggcggag agtatgcagg aaaagtattg
    20341 gacgacatgc ttgtatccgt tccttacaca gatcagccac cgctcgaagg ggttcgttca
    20401 gatggcgaac cgctaacgat tgctgatcag atgtttgatc ctaaattgaa ccaatggatt
    20461 attttagcga acgcgttaga tcacaacgat ttaaacaatc tcaaagcgat gtacgaggct
    20521 cttgaacatg aaaacggcaa cctaaaacag ctaaatgcca aactcatgct aagcgatgta
    20581 gcgattaaac aggaaaatac tgcattgaaa gaaaaagctg acagtttagc acaaatcaat
    20641 tcaaaaatga tgcttgcttc gttacaaaat agcaaagaca tttcagaaat taaagagcaa
    20701 ctaaatccag cttcaaaggg aggtgagtag tatgtttagt tttagcgatg tgaaaatgat
    20761 gtatgattgg ggctgtttta ctgacgatca agttcgtcta ttcgttccac tatgtatcac
    20821 agatgaagaa gcagatagaa ttattagcaa agaagagagc gcatcttaag tgatgcgttt
    20881 ttttgttgga aagttggtgg aacatgaaag aagaagcgct ccaagacgtt gtggagagat
    20941 tagtaagaat tgaaacaaaa ttagacaact acgaatcact tagagaaaaa gctgatagtg
    21001 caaaagattt ggcagacaaa gcctactcag tagcgctaaa caacgcagaa gatatcaaag
    21061 aaatgaaagc caataataaa tggtcgtggg gttacatgat cggtttaggc attacgatca
    21121 ttggctattt cttgactaaa ttgtaaagga gttaagaaga aatgatttta cccgataagt
    21181 attatcaagt cattaaatgg acggttttaa cagtattacc agctgcatct gttttagtag
    21241 ccacgttagg aaaagcgtat ggatggaatg gaacagatat gacagtactc actatcaatg
    21301 cagtagcaac atttttaggc gttatcactg gtgtgtctgc atataactta aaaaaatagg
    21361 aggaaaaaaa tgaagaagaa aatcatttta tcattgagcc taataatggc tcttttttta
    21421 ttgccaatta acgggttcgc ctatacgatc aacaatgaat ttaatttagg tgtaaatgaa
    21481 ggtagctcac aagtagcaaa taaccaatac atcctattgc atgaaacagc aaacgaaact
    21541 gcgacaggac gaaatgaagc gcagtacatg aaacgttctt ggtacaatgc ctatacagcg
    21601 tatattgttg gcgatggtgg gatcgtttac caagttggac aacctggtta tgtacaatat
    21661 ggcgctggtt catatgctaa tgcaaatagt cctgtgcaaa tcgaactgca acatacgcac
    21721 gataaagtaa cgtttgaaaa gaactacaag gcatatgttg aattagcgag agattccgct
    21781 aataaatacg gtattccact tacattagac acaccgtata atcaaccagg aatcaaatca
    21841 catttatggg taactcaaaa catttggggc gatcacactg atccgtatgg atacttatca
    21901 gaaatgggtg taagtaagga aaaactggct tacgatttag ctcatggatt tacagacgaa
    21961 aacccaacga cttctgagaa caagcctgtc attgatccaa cccgagcagg tgcagcaaat
    22021 ccaactttat cagatggaac gaaccattct cacattgatc agtttggaga aatcgaaaat
    22081 gcgaacttac acgtcgctgg atggcacatc gctaactata aatacgagta tattttcatt
    22141 atggactaca atactgggaa agaattagct cgagtaagag ctgatggaat ttatagaccg
    22201 gatgtaaatc aagcttataa tacttctgga aacgttggct atcatgtatc ttttaacatg
    22261 cgcaatttcc ctaataagaa agtatacgtc atgatgcggg caacgaatga tccagaagga
    22321 aacactaaag gcggtgcgca agatttccat gacaaacgtt ggtatttaaa tattcctaaa
    22381 cgataaaaat agctcctcgt tgaggagcag tacatagaat tgaaaattaa cgttaaatca
    22441 aaaaaatatt tacaagatgt ttatttaagt agataataaa agaaaaaagc aggtgaaatt
    22501 gtgacagcag aaattggtat tatgaataaa agtggaatcg tgttagcttc agattcagca
    22561 tctacgattg gagatagcaa agtatataat actgcaaaaa aattatttac tttggattcc
    22621 atgcattctg taggtatcat gatatatggt aatgctgaat ttaatggtat tccttgggaa
    22681 ataatcatta ctcaatataa gaaaagcatt ggtagttctg tttttaatac tttggaagaa
    22741 tacgcggata attttataga atttgtcaaa acagcatctt ttattagaag tgaacagacc
    22801 gagcaagaac aaatgattgg ggtttttcaa aaaataattt caggtttatt tgagagtata
    22861 gaagtagata ttaacttttt aataagccaa ggtacacaaa tagacaaaga tgtcctagtg
    22921 aagttattac aaacaaaaat gaataccaat ttgtctcaac agtcccagac attcatttta
    22981 gatattgaaa aggcgttatt tcttagtaac tatggagaaa
//
LOCUS       DAZKMB010000036        22719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000036 DAZKMB010000000
VERSION     DAZKMB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.036"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1942)
                     /locus_tag="ACG8V3_001246"
     CDS             complement(11..1942)
                     /locus_tag="ACG8V3_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0299794.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG8V3_001247"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG8V3_001247"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0299795.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="ACG8V3_001248"
     CDS             complement(2871..3623)
                     /locus_tag="ACG8V3_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0299796.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="ACG8V3_001249"
     CDS             complement(3782..3991)
                     /locus_tag="ACG8V3_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299797.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="ACG8V3_001250"
     CDS             complement(4002..5330)
                     /locus_tag="ACG8V3_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299798.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="ACG8V3_001251"
     CDS             5466..5621
                     /locus_tag="ACG8V3_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0299799.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG8V3_001252"
     CDS             complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG8V3_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0299800.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG8V3_001253"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG8V3_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0299801.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG8V3_001254"
     CDS             complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG8V3_001254"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0299802.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="ACG8V3_001255"
     CDS             complement(9305..10237)
                     /locus_tag="ACG8V3_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299803.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="ACG8V3_001256"
     CDS             complement(10394..11053)
                     /locus_tag="ACG8V3_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0299804.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG8V3_001257"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG8V3_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0299805.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="ACG8V3_001258"
     CDS             complement(12094..12384)
                     /locus_tag="ACG8V3_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299806.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="ACG8V3_001259"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="ACG8V3_001259"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0299807.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG8V3_001260"
     CDS             complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG8V3_001260"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0299808.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16049..17218)
                     /locus_tag="ACG8V3_001261"
     CDS             complement(16049..17218)
                     /locus_tag="ACG8V3_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0299809.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG8V3_001262"
     CDS             complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG8V3_001262"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0299810.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG8V3_001263"
     CDS             complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG8V3_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0299811.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22182..22703
                     /locus_tag="ACG8V3_001264"
     CDS             22182..22703
                     /locus_tag="ACG8V3_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0299812.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6326 a         4932 c         4041 g         7420 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc tttgactact
    14701 gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc tgccatcact
    14761 cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga tcccatttca
    14821 gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc gtttttatca
    14881 cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc aagctctgat
    14941 aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag catcccatct
    15001 ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc aaagttataa
    15061 tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc
    15121 aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt cgtaacgatc
    15181 gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa catgatttta
    15241 tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc atcgccatcg
    15301 aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg ttctggatgg
    15361 gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat tgtataaaaa
    15421 tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc gtttgctgca
    15481 tcgacacaaa tggtcaaacc ggaaaggtca ccgttaatcg tttgtacaag gaattgagaa
    15541 tatttcaata agccttctgg aaattcttct acagttccca atccttcagc tgatggacga
    15601 ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg gtcatctact
    15661 agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg attatgagaa
    15721 gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc aacacctggt
    15781 gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc tgaaatcagc
    15841 gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac gcgtgggcgt
    15901 tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag cttaaatgca
    15961 agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc aaaatattta
    16021 cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt ctgctcattt
    16081 gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag
    16141 attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca gcagatgaaa
    16201 tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca gagctactgc
    16261 ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg atacttaacc
    16321 cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct gagacattga
    16381 tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg cttacttctg
    16441 cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt gttccttgct
    16501 gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc actgttactt
    16561 gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc tgtattggga
    16621 cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct actactcgtt
    16681 tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga ctaacggaag
    16741 tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt tccacatcga
    16801 aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg atctttgctg
    16861 tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct tcgcccagct
    16921 tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct gcattcaact
    16981 gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca tggatatagt
    17041 agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga ttatacgctt
    17101 catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac agcgcaatac
    17161 tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt gagatcattt
    17221 gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt tcttgttttt
    17281 tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt gctgggataa
    17341 gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt cagagacaac
    17401 gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc gagtcccgaa
    17461 ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta ctgcgatctt
    17521 tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa agatattgat
    17581 cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat actcatctaa
    17641 tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca tatattggat
    17701 ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt ttgattgttt
    17761 gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct gaaaaatcac
    17821 gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca aggtgtgcag
    17881 accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt tcagaagctg
    17941 aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc agacaaccag
    18001 aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa acatttgctg
    18061 ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc tctcttctct
    18121 tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat atgctcgtat
    18181 ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt tatttttctc
    18241 tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg ttatttttcc
    18301 tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc agctcttcta
    18361 gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt
    18421 acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt
    18481 gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa taaagctttt
    18541 gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct catgaaatca
    18601 ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt gtttttttcg
    18661 accattgtag ggttatatcg atataatgac caataaccag aagaaacagc ttcttttgct
    18721 tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg cgtataggca
    18781 ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat cgtttgcatt
    18841 tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat cgccatcatt
    18901 cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat tgccgaagct
    18961 ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc caaaacaagg
    19021 atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa
    19081 gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc ctttctcttg
    19141 tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt
    19201 gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc ttccatcaat
    19261 tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc caaagcctcg
    19321 cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc ttccaataaa
    19381 gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga agcaggagaa
    19441 gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg atcaccgaac
    19501 atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat
    19561 tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt gactggattt
    19621 gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt ctgttcttca
    19681 taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca cagaccacaa
    19741 cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc gcctcgcatt
    19801 tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc taaaaatgga
    19861 cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca gcgatcacta
    19921 atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt tcctaaaggc
    19981 atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg atttccttcc
    20041 tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt attgattttt
    20101 tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag cagttcggtc
    20161 gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg tccatagctt
    20221 ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat gtcagctaat
    20281 ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc
    20341 aatctcatgg cattgatcgt atagaatcta atgttatttt cagccaagta acgttttaat
    20401 tttaagggca gatgcgtttc taattgttcg tcgctccaaa gagtattcaa taaaaaaatg
    20461 ccacctggct tcaatccttt gaccaagtca tagctatgca ggtaagctgg tgtgtgacag
    20521 gcaacaaggt ccgcatgctc gacaagatac gctgaacgta tcggcgtatc tccaaaacga
    20581 agatgagaga cagtcagacc gccagatttt ttcgaatcat aataaaaata gccttgcgca
    20641 tatttatcgg tgtgatctcc aataatcttg atagctgatt tatttgctcc aactgttccg
    20701 tcagatccaa atccccaaaa cttggcctgg aatgtttgcg gttctgtcaa atccagactt
    20761 tcctttggtt ccaaagattg ataagtcaca tcatccacga tccctatggt aaaccgtttt
    20821 ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg ctgagatttg gtcgggagtc
    20881 acatctttag accccaatcc gtagcgacca ccaataacag ccgggcgaag ttcagaatca
    20941 tataacgcac tttgaacgtc taataagaga ggttctccac ctgcccctgg ttctttactt
    21001 cgatcaagaa cagcaattgt ttttactgta tgaggcaact tttgcacgaa tgtttccact
    21061 ggaaaaggac gataaagatg gatattcaaa aaacctgttt tccgtccgtt tgcattcaag
    21121 taatcaatcg tttgttcaat cgtctgtgca actgatccca tagaaacaat gacttcttcc
    21181 gcatcgtctg ctccgtaata agtcaccaag tcataagaag ttcctcgtaa tcggttgatc
    21241 tctttcatat atttttgaac gatttcaggt aatttatcat aataggggtt gatcgtttcc
    21301 cgttgttgaa aatggatgtc tggattctgg ttcgtaccac taacagtcgg atgattggga
    21361 ttcatccctc gtttacgaaa tgaagctaat ttctcctgat tgatcagagg agctaattct
    21421 tcatattcca aaacttcgat tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa
    21481 ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg caacaggtgc taaatccatc
    21541 acttcttgta cactgctttc agctaacata gcaaatcctg tttgtcttgc agccatcacg
    21601 tctccgtgat ctccgaaaat attcaacgca tttgtcgtca ctgcccgact agcaacgtgg
    21661 aaaacacttg gcagtagttc cccagcaatc ttgtacatat tcggtatcat caaaagcagc
    21721 ccttgtgaag cggtatatgt agttgtcagt gcaccagttt tcaatgatcc atggaccaca
    21781 ccagctgctc ctgcttcgga ttgcatttct actactttta ctggttgacc gaatacgttt
    21841 tcttttcctg ctgctgacca ttgatctact aattcagcca tggtcgaact gggtgttatt
    21901 ggatatattg cagcaatctc ggtaaatgca taagaaatat atgcggctgc cgtattgcca
    21961 tccattgttt tcatttttcg catgattcat catccttgtt catattctct cttattatct
    22021 gttccattat aacgtaaaac atgccatttt tttcttttat ttataaaaat attttacatg
    22081 tatctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    22141 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    22201 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    22261 aaatggtcga attcatcaac ttagcttgtc cgtattgccg acaatggttt gaagaatctt
    22321 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    22381 ataaagaaaa agaaagctta cagcgaggga acgtgatgca ccggtatctc acaatcagcg
    22441 atggccaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    22501 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    22561 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    22621 ttccaactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    22681 aagaattgat ccaaacaaaa taaaaaaata aggagcgtg
//
LOCUS       DAZKMB010000037        22160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000037 DAZKMB010000000
VERSION     DAZKMB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.037"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="ACG8V3_001265"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="ACG8V3_001265"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0299813.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="ACG8V3_001266"
     CDS             2370..3848
                     /locus_tag="ACG8V3_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0299814.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="ACG8V3_001267"
     CDS             3814..5145
                     /locus_tag="ACG8V3_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0299815.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="ACG8V3_001268"
     CDS             5259..7811
                     /locus_tag="ACG8V3_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0299816.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="ACG8V3_001269"
     CDS             complement(7818..9257)
                     /locus_tag="ACG8V3_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0299817.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9232..9414
                     /locus_tag="ACG8V3_001270"
     CDS             9232..9414
                     /locus_tag="ACG8V3_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299818.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9603..9965
                     /locus_tag="ACG8V3_001271"
     CDS             9603..9965
                     /locus_tag="ACG8V3_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0299819.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10152..11009)
                     /locus_tag="ACG8V3_001272"
     CDS             complement(10152..11009)
                     /locus_tag="ACG8V3_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0299820.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11129..12040
                     /locus_tag="ACG8V3_001273"
     CDS             11129..12040
                     /locus_tag="ACG8V3_001273"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0299821.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12047..13693
                     /gene="mdcA"
                     /locus_tag="ACG8V3_001274"
     CDS             12047..13693
                     /gene="mdcA"
                     /locus_tag="ACG8V3_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0299822.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13795..14094
                     /locus_tag="ACG8V3_001275"
     CDS             13795..14094
                     /locus_tag="ACG8V3_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0299823.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14084..15760
                     /gene="mdcD"
                     /locus_tag="ACG8V3_001276"
     CDS             14084..15760
                     /gene="mdcD"
                     /locus_tag="ACG8V3_001276"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0299824.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15764..16357
                     /locus_tag="ACG8V3_001277"
     CDS             15764..16357
                     /locus_tag="ACG8V3_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0299825.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16381..17457
                     /locus_tag="ACG8V3_001278"
     CDS             16381..17457
                     /locus_tag="ACG8V3_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0299826.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17482..18351
                     /gene="mdcB"
                     /locus_tag="ACG8V3_001279"
     CDS             17482..18351
                     /gene="mdcB"
                     /locus_tag="ACG8V3_001279"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0299827.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18462..19226
                     /locus_tag="ACG8V3_001280"
     CDS             18462..19226
                     /locus_tag="ACG8V3_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0299828.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19715..21808
                     /locus_tag="ACG8V3_001281"
     CDS             19715..21808
                     /locus_tag="ACG8V3_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0299829.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21833..22135
                     /locus_tag="ACG8V3_001282"
     CDS             21833..22135
                     /locus_tag="ACG8V3_001282"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0299830.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7177 a         3786 c         4721 g         6476 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9421 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9481 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9541 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9601 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9661 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9721 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9781 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
     9841 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
     9901 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
     9961 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10021 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10081 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10141 ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat cattttgatc
    10201 aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa gggcaaatct
    10261 ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac ttttgggaaa
    10321 aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc atctgtcgtc
    10381 gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt atgacggaag
    10441 gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa ttgcagatgt
    10501 tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc tttcatgata
    10561 ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg atcggtaata
    10621 tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt ttccataaaa
    10681 attcggacca agttcggaat agaaacagag gctacctcta aatacaatat gacggaagta
    10741 acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt gtctaatgtc
    10801 ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt tctgccgcta
    10861 tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg gctcaaggca
    10921 ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc gaatactttt
    10981 ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac ctttttgtta
    11041 tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga tagcagtgga
    11101 aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc ctggacaagg
    11161 cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac gtttagaaaa
    11221 tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg tttttatcca
    11281 gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa acggtattca
    11341 gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg cttgtcgatc
    11401 tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa tgaaagaagc
    11461 ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg aagctgagga
    11521 aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg aaaattgtcc
    11581 gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg cagaagccaa
    11641 acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc actgtctctt
    11701 gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc tatctgatgc
    11761 tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag aagagattcg
    11821 taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga tggacttggc
    11881 aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt tgacaaagct
    11941 gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag accaacatgg
    12001 tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg aaaaaccaaa
    12061 ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa tcagcaactt
    12121 gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa aactgattgc
    12181 tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt ttctttcaga
    12241 aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca tgtctagtat
    12301 ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga tcgatttctc
    12361 ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca caatgaaatt
    12421 aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt tgattccaaa
    12481 tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca caggtttcaa
    12541 tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta tcgtgatcgt
    12601 tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg actgggtaga
    12661 cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac gagatcctca
    12721 aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa tctacgcaga
    12781 acacggcgta caatctttga atcacggtat tggattcaat acagcagcta tcgaattgtt
    12841 actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga actgggtatt
    12901 gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa gcatccatag
    12961 ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg tcttttttgt
    13021 cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag gacaatatgc
    13081 catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact cttctaccgt
    13141 aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata atcctggcgg
    13201 tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc cattagggaa
    13261 aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa aaccagtctt
    13321 tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca atgcaccagt
    13381 gatgatttat agcgaagata ctacacatat cgtgacagaa gaaggaattg cttatttgta
    13441 caaagcaaaa acaaaagaag aacgacaagc agcaattgca gcaattggcg gtgttacacc
    13501 tcttggaatg actagcacga aagaatcact agatagcttg cgtgcagccg gcatcgtgaa
    13561 attaccagaa gatctaggca tcaaacgtag tgaagcgaaa cgttcattat tagctgcaca
    13621 aaccatcgat gatttgatcg aatggtcaga tggcctgtat gaaccaccaa tgaaattcag
    13681 aacttggtca taaaattggc gagaaaaacc ttttgtcgcc atttacccag atggaagaag
    13741 aaagacgaat aagcagaacg tgtatgctct gttttacaaa aaggggagag atccatggaa
    13801 caattagctt ataaatatcc aacgaaacag ccgatcaata agaaaatcca tgtaggagtg
    13861 gtcggttcag gagatttgga aatcttgatg tttccaacag aaaaaacaga atctactgta
    13921 aaaatttgta caggaagcga tggcttcggc gaagtatgga acaatgtatt aacgagattt
    13981 tttgatcgtt atccaatttc agcagatatc gtgatcaatg acttcggtgc tacaccagga
    14041 gttgtgtacc tacgattgac gcaagcaatg gaggaacttt cagatgaaaa atagttttgt
    14101 agaattaaac gccagagaac gagtgaacgc tctacttgac aatggacgtg aattgattga
    14161 tccattcgat caaatcatcg cgccaagttt accagcacaa ggaatcgtcc ctgaaagtga
    14221 tgacgggatc gttgtcagcc gtggcacgat caatggcaaa caagcagtag taatcgctat
    14281 ggaaggaaaa ttccaaggtg gcggtatcgg tgaagtttcc ggtgcaaaaa tcgttgctgc
    14341 acttgaacat gtgttagaag aaaataaaaa aggaaatgag atctttccag tcattgtact
    14401 agatacaggt ggtgttcgtt tgcaagaagc aaactacgga ctgttatcta tttcagaaat
    14461 cggaaatgta atcattgctc taaaacaata tgtaccagtc atcggtttga ttccaggacg
    14521 tgtaggatca tttggcggta tgtcgattac ttcagcatta atgagttatt taatcgcaac
    14581 aaaaaaagcg agagtcggtt tgaatggacc agaagttatt gaacaagaag caggcgttcg
    14641 agaatttgat tctagtgaca aagacttgat ttggaataca ttaggatctc gtcaacgggt
    14701 agaatcaaaa atcattgatg aattagtgac tgatagtgta gaagcaatca aagctgctgt
    14761 catcgcagca atggacgaac gaaaagacac gcatcgcagt gaaagctcag cattttattt
    14821 atcattactt gattctttag atctttcaaa accaatggcc attgacgatt acaatgaagc
    14881 aattgctaac catcaagcaa aatcaattga cttaccagaa gtagcagaag gaaatgaacc
    14941 agctgcagca agtgttggtt acgattggtt tgcggcacta actggcatga aaaatccaac
    15001 ttcttctatt agtactgtgt attatggtaa atcaagtaaa ttcatagaag aagccatcgt
    15061 gacatcaatc gtacctgatc ctaaaaaccc gatgtaccgt gtacgaaacg gtgaagttgg
    15121 tttagttgaa ggtttccgca tgggacaaat tttggatcac gtttacgaag aagataaaga
    15181 caaaaaagtg aaacggccaa tcatcgttgt gatcgatgta ccaagccaag catacggcta
    15241 taacgaagaa ttgatcggta tccatgtggc tttggctaat agtgctgctg cttatgcaaa
    15301 actccgtcaa gctggacatc cagttattgg attgattgca ggaaatgcta tttcaggtgc
    15361 atttttagca catggattac aatcaagccg cttgattgct ttaaacaata aagaaattac
    15421 cgtccaagcg atgtctaaag aaagtgctgc acgtgtgaca aaacgttcgg ttgctgaaat
    15481 cgaagcagct gctgaaaaag ttccagctat cgcttacgat atcaataatt atacaaaact
    15541 aggagcgatc tatcatttcc tagaagatat caccgaccaa gctgcttcag aacaaaatgt
    15601 cagcaaagtc atcgaagcaa tcaatgaagc cattcaagat gtccgcaata caaatgacac
    15661 gatgctgaca aatcgataca caaacgaaac agccaaaaca ttcggacgtg tcgaaacaaa
    15721 taaagtccgt gccaaaatgg acgaagaatg ggatgcataa aaaatgattc aagcacatga
    15781 tattgtcctt tttgataaac atgaaataga agggttcacc ttgcctgaat gggtcgttct
    15841 aactgaaaag atgttcggaa cagttcgtcg tgcgtcgacg gatcacgcag aaatactacc
    15901 gattggttta cgtggacctg cccgcaatca gagatggtca acagaaatct caaaacatgc
    15961 cgtcaaacaa gtcattcatc cgtgggacat catcgaacaa gaatcgtata gacaagagaa
    16021 aatcatggat tatcctgttt atcagcaatt tcatgctgct agaaacatat tatcaggatg
    16081 taaatgggga atcggcggca gcttaggatt tgaattatcg actgggattc cggcagtaaa
    16141 agaaaccagt gattttgatt tgctgctgta cgcaaattca ccgattgaat tgccaattca
    16201 ggtaattcaa tcgcatcctg ctttttttga gcaattcgat acccaagtag tgactcggca
    16261 aggcggtttt tcactaaaag agtatttgag agagccagag aaaaaaattc tattgaaaac
    16321 agtcgaagga cctaaattaa ccaaagaact ttggtaataa agaaaaagag agagaaagag
    16381 atgtttgaaa acaaaaaaat caaaatcaac gtaagtgact tccagaaagt atcagctgta
    16441 acggctgtta tgatgcaaac gatattgagc tttgctttgg caaacagcca cagcgcaaca
    16501 aatacagctt ttcttggtac cttttacgtc atcgttaaat acacagcacc aatgtttatt
    16561 tttgcaattg tttataatat ggtaaaaaca agtcagcatt tgtcttattg ggaatttttg
    16621 aaagaaaagt tttttgaact agtcgttccg tatcttctat ggacgacggc ttatctttgg
    16681 ctatttccag cagtccaaca acaaactccg tatacggata ctgcaagctt tttactgaaa
    16741 tatgtcacag gtgatggagc gcctcattta tggtataccg ttatgatgct gcagatccaa
    16801 ttgttcatgc cgttttttgt ttggctcggt tacaaagtat ttgctaataa aaaatatgta
    16861 tggccagtat tgattggttc aacgatttta tatgtcgcat ggtatctgtt ctatcaaaca
    16921 caagtattga ctgggcctta tcatgacagc tggtacttat tagatcggtt tgttgctagt
    16981 ttcatgattt atggggtagc cgcatttgtt tatcacgaaa aagtctatca atatttggac
    17041 agagtacgtt atcttttctt acctgttgcc ttagttatcg cgttcttttc tgtccgttca
    17101 ttgctcgcac atccaggaga cttgagcttt gcgaatgcac cttacttgaa tacgatccaa
    17161 agtttataca gtttagtcat catctttgct gtcttcatag gcgcttccaa aatgattgta
    17221 aacgattcgc caaaattgcc attgttcaaa tggttgtctg tttatgctta tcgtacgtat
    17281 ttagccaatg tatttgtttt ccaagtactg ttattattgt ttaaagatag ttggctgcag
    17341 ttaccttcag gagtcatgat tcttgtagct tatctaatga cggcaagttg tgcatttgct
    17401 ttaagctggt tacttcatat catttgggta gctatcaaaa aaggtttcag caagtaatag
    17461 taggaagaag gagtgagtgt catgataaat gacgaacgaa aaaaagcagt gactcagcaa
    17521 ctcgctgcgt tggtagagga agcattgatc gcagaagtaa cattaagtcc aaaacctgga
    17581 ttagtggatg cactggatgc gggtgcacat tccgatatgg actatgcttt atttcttaag
    17641 tctgctaaga cgctaactcc tttttttgaa gaaatggctc aaatagcatg gcatcatgct
    17701 attgatcagg agttaagaga aagaattgct gagattgggc gaaaagcgga aaaggcaatg
    17761 ctgattgcta caaatggcat caatacccat aaaggagcga tttgggcaat ggggctattg
    17821 atcagtgtca ttgcacaaaa aatcagtatg gaacagcctt tttctttatc gaaaatactt
    17881 gatgatgtca gcaagcttgc tgcttttcct gatacaaaat atcgtcataa aaaaaccact
    17941 catggtcata gggtgaagca aaaatatggc gtaaatggtg catatgaaga agcgatatta
    18001 gggtatccgc atatcaggct tgcaattcaa gcagctaagg agctagataa tgattcatat
    18061 gtacagaaac aaatgcatat gttgttagtt ttgatgagtt cgctagatga cacatgcgta
    18121 ttgcatcgca gtgatcagaa aacgttagtg gaaatgcaaa aacttgctca tgctgctagt
    18181 caaagtgctt taccaaatcg tccatttaca gcactgttgc acttttgtca ggaaaaccaa
    18241 atctctcctg gaggaagcgc agatctcttg tctgcaagct tgttcatgct gaagatagag
    18301 gaattatgga ctgccgacac atacatttgt ccgatgttac tcaatgacta aataagacaa
    18361 aattgctaaa aaaacagttt atgcatctgg ttattgactc ttccttttag caaggctatc
    18421 ataaaaatag ttttttgtaa taaaagataa cgaggaagac aatgaaaaaa atcaattatt
    18481 tagcaatcat tttgttagtg ggcctatgtt tgtttgctct gccgacccaa ggattcgctg
    18541 tcgcgggtgg tcatggtgga ggtacatcta gcggtcgaag ttcgggcaga ggttcatttg
    18601 gtggtcgttc atatgggggc agttattttg gcagtcgttc ttacggcaga gattcttatg
    18661 ggtattatgg tccaggtaca ggtccttcta gttatgagca aacgggattt cttgcattag
    18721 ctgttttgtc agtaggaggg gtttctgctt tttctatcgt caagaataaa agacaaaaga
    18781 aaaggctgaa agcggagata ttgtcccgca tgtcaggaac ggctaaagaa aagaaagcac
    18841 agtttctgga aatccaaaag atattcatga agatccaatc cgcttgggaa gaagaaactc
    18901 ctgaaaaagc cagaagctgt tatacagaca agctgtttgc cgaacatcag aaagttctgc
    18961 aaaaaaacaa agcagacggt gtaagaaatc atactaaaaa aataactatt caggaagtag
    19021 gaaattatcg acagatccac gaaaatagtt ttgccatccg aattgatttc agatgctgtg
    19081 attattcagt cgatcgaaaa gatgggcgat tgatctcagg aagtaagaca caacgacagt
    19141 atttttctca gatatggtat tttgattttt ctgagaaaga aaaaagatgg caagccgatt
    19201 ttattcagcc gattatgctg gattgacagg aaatatttat gaacggagtt gtgacaaaag
    19261 ccttgtcgca gctccttttt ctgaataaac ggtgtgccag agatagccta tcggcaataa
    19321 ggcacgattt tgtaaaatat gggaagcgat ttttcaaaat cgcttcttat cgctcaaggc
    19381 tgaacatctc tgtcacaacc tctccactaa atggtgttta aaagcttgca tctgcgataa
    19441 taagaacaac caaacgaaaa ataggaagca ctattttagt ttagttgttc ttattacttg
    19501 atgcaggaca gctttttcac aacctctttt ttgtatagat gggcaaaatt tttgtaataa
    19561 taggcgtatg tctttttcgt tgaggagatg aaaaagcata tggcttattt tttgtttgct
    19621 ttgcttactt tctttccact tcaagtggca gaaatatcta ctttttatta tgtaagcgga
    19681 accatatact tgaaatcaga aggagatcat ttctatgaaa acaagaacaa ttgaaatact
    19741 tcacagaatc atgacagccc aatatccagt aaaagcaaaa catctagcaa gtgagttcaa
    19801 tgtcagtcag cggacgatcc gtcaggaagt tcttgaagcg aatatctggt tgagcagtaa
    19861 aaagcttccg gagatccgta caattcgcaa caaaggtttt ttgttgaagc tggaaaagga
    19921 aaagcaagct cgattagaaa ctgaaataaa agatgtgaaa gacggctttt tgaatcggcg
    19981 cgaacgtatt tttgatctag ttttagccgt tgcttacagc cgaatcccga tccatctaaa
    20041 caaaaaagaa gaagaatttc aaatctctaa aagtacatta gatgaagata tgagacggtt
    20101 acgagcaaca ctggtcagtt atgggataga gatcgtcagt tatggaaagc aaggactggt
    20161 ttataaaggt tcagaacgat ctatccgtac gatgatctat gattttatca atcagaatct
    20221 gggaatgacc gacttcttta tgaatcaaga tgcacaatta acaccggctc aaaaaatctt
    20281 tctccattat tttcctttgg tggaaatcaa aaaaatacag gagatctatt ccaataagtt
    20341 gttgaaacaa gaagatgata tttataaaaa acaaatcctg ctttttacat tgatctggat
    20401 ccagcgaatc aggaaacatg aatttatctc tgccattaat tggaaaaata cagaaataaa
    20461 agacagtagc ttttctgcat ttattgacca catgatcgag gaatttcaat tggacaatgt
    20521 cccgcaagta gaaagaaatt atcttcgttt cactattgag acgttcaatg ctagagatat
    20581 ctccaattca atcgagtggg tacaagctca attactaacg atccaattga tccaatttgt
    20641 agagaacgag acacacgtac ctttccatct aaaggaagaa gctttattcg aaaatctcta
    20701 taagcacatg gctgcactga ttgtaagaat caagaatcat atccaagtca tgaatccatt
    20761 gaaagaaaac atccgaacaa attatttccc aatctaccgc gcagtcactc attttgttcc
    20821 tacgatcgaa gaagtaattg gtggcaagat actggaggac gagatagctt ttcttgtcat
    20881 ccacttttcg acgatcgcca gtgtaatcaa gcgagacttg aattatgttt ataaatcagt
    20941 cgttgtctgt aaccatggga tggctactgg aaatctgtta gcagagaatc tgaaagaaaa
    21001 gtttcctcag atcgagatta ctgcagtttt aagttcaaaa gaaatcgaat tagtagataa
    21061 attggatgtc aatctggttt tcagcacctt tcaattgaat tatcgaaata agccgctttt
    21121 agttgtcgat ccgattttaa ctgagacgaa tatcttggtt atagaagact ttttgaaaag
    21181 aaatgaagaa ctacagcggt tagagcctaa cagtcatgaa tcgacggagt tattcactca
    21241 aataatagat gtcatgaaag aaagtggtgg ccgagtagat cgaatcatct atggcaagtt
    21301 agaaaaatta ttcgaaacaa acaatttaga aatcaacaaa agggagatcc agcctatgtt
    21361 aaaagatatt ttgacggaca gcaatatcat gatcaaagaa aaagccacta cttgggagga
    21421 atcgattgaa ctagctgcta gaccattggt taaagaaaac attgtcgaag agcgctatgt
    21481 cgaagcgatg attcgtgcag tgaatgatta cggaccgtat atcgtgattg gcaaacacat
    21541 ggcactagcc catgcccgac cagaagatgg ggtcaataaa ttaggagtca gcgtagctac
    21601 gattgagcaa ccaatcgatt tcggtaatcc tgaaatggat ccagtaaaga ttattttttg
    21661 tttggcagca gtagactcct actcgcatct gaacatcatg aaagaactaa ttgaattgat
    21721 caacaacgaa gagaagttgg atcgattgat tgaatgcaca aatatcgaag atttcaaaca
    21781 actgcttttt tcagtagttg agaaataata aatgatacgg gggagaacaa agatgaacaa
    21841 attgaacatt ttattcgtat gcggtgcagg tttaggaagt agttttgcag cacagatgtc
    21901 tgctgaggat gttttgaaca aattaggagt agaagccaaa ctggaccatt gtgatatttc
    21961 ttccgcagtt tccatgaaac cagatgtgat catcacagca gaaaattttc gttcgcagtt
    22021 cgaaaagttc gcagttgatc ctaaaacggc gatgatctat ttgaaaaata ttgtttcaaa
    22081 aaatgaaatt gaagaaaaaa tcaccccagt tctgcaagat aagggagtta tctaaataca
    22141 aaaagaaaag agggtaaaaa
//
LOCUS       DAZKMB010000038        22089 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000038 DAZKMB010000000
VERSION     DAZKMB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.038"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..1917
                     /gene="efbA"
                     /locus_tag="ACG8V3_001283"
     CDS             211..1917
                     /gene="efbA"
                     /locus_tag="ACG8V3_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0299831.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2075..2977)
                     /locus_tag="ACG8V3_001284"
     CDS             complement(2075..2977)
                     /locus_tag="ACG8V3_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0299832.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2974..3666)
                     /locus_tag="ACG8V3_001285"
     CDS             complement(2974..3666)
                     /locus_tag="ACG8V3_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0299833.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3709..4350)
                     /locus_tag="ACG8V3_001286"
     CDS             complement(3709..4350)
                     /locus_tag="ACG8V3_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0299834.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4388..4702)
                     /locus_tag="ACG8V3_001287"
     CDS             complement(4388..4702)
                     /locus_tag="ACG8V3_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299835.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4690..5319)
                     /gene="pyrE"
                     /locus_tag="ACG8V3_001288"
     CDS             complement(4690..5319)
                     /gene="pyrE"
                     /locus_tag="ACG8V3_001288"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0299836.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5326..6042)
                     /gene="pyrF"
                     /locus_tag="ACG8V3_001289"
     CDS             complement(5326..6042)
                     /gene="pyrF"
                     /locus_tag="ACG8V3_001289"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0299837.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(6039..6971)
                     /locus_tag="ACG8V3_001290"
     CDS             complement(6039..6971)
                     /locus_tag="ACG8V3_001290"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0299838.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6968..7744)
                     /locus_tag="ACG8V3_001291"
     CDS             complement(6968..7744)
                     /locus_tag="ACG8V3_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0299839.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7798..10983)
                     /gene="carB"
                     /locus_tag="ACG8V3_001292"
     CDS             complement(7798..10983)
                     /gene="carB"
                     /locus_tag="ACG8V3_001292"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0299840.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10988..12064)
                     /locus_tag="ACG8V3_001293"
     CDS             complement(10988..12064)
                     /locus_tag="ACG8V3_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0299841.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12069..13349)
                     /locus_tag="ACG8V3_001294"
     CDS             complement(12069..13349)
                     /locus_tag="ACG8V3_001294"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0299842.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13368..14294)
                     /locus_tag="ACG8V3_001295"
     CDS             complement(13368..14294)
                     /locus_tag="ACG8V3_001295"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0299843.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14307..15590)
                     /locus_tag="ACG8V3_001296"
     CDS             complement(14307..15590)
                     /locus_tag="ACG8V3_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0299844.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15604..16143)
                     /gene="pyrR"
                     /locus_tag="ACG8V3_001297"
     CDS             complement(15604..16143)
                     /gene="pyrR"
                     /locus_tag="ACG8V3_001297"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0299845.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16378..17289)
                     /locus_tag="ACG8V3_001298"
     CDS             complement(16378..17289)
                     /locus_tag="ACG8V3_001298"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0299846.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17286..17750)
                     /gene="lspA"
                     /locus_tag="ACG8V3_001299"
     CDS             complement(17286..17750)
                     /gene="lspA"
                     /locus_tag="ACG8V3_001299"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0299847.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17755..18453)
                     /locus_tag="ACG8V3_001300"
     CDS             complement(17755..18453)
                     /locus_tag="ACG8V3_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0299848.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18440..18826)
                     /locus_tag="ACG8V3_001301"
     CDS             complement(18440..18826)
                     /locus_tag="ACG8V3_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299849.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18973..20643)
                     /locus_tag="ACG8V3_001302"
     CDS             complement(18973..20643)
                     /locus_tag="ACG8V3_001302"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0299850.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20826..21059
                     /locus_tag="ACG8V3_001303"
     CDS             20826..21059
                     /locus_tag="ACG8V3_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0299851.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21158..21589)
                     /locus_tag="ACG8V3_001304"
     CDS             complement(21158..21589)
                     /locus_tag="ACG8V3_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0299852.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21668..22081
                     /locus_tag="ACG8V3_001305"
     CDS             21668..22081
                     /locus_tag="ACG8V3_001305"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0299853.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
BASE COUNT         6252 a         4932 c         3899 g         7006 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgataccta
       61 aaaatttgga tttcaacgat agaaacactg ttttttatgt aattagtcca agatttttct
      121 agacttttgc tttactattc ggccaaattg gtttttcttc tttttttcgt tataatgaat
      181 atacttatga aaagaaatga ggaatgaacc atgtcctttg atggtgtatt tacccacgcg
      241 atggtcaacg aattgcgtga aacgttacta tctggccgga tctcgaaaat ccatcagcct
      301 tatgaaaatg aaatcgtgct ggtgattcgt tcacgcggca aaaaccatcg attattatta
      361 tctgcgcacc caagttatgc tcgtgtacag attacccaaa tcgattatca gaatcctgat
      421 aatccgccta acttcgtgat gatgcttcga aaatatttgg atggcgctat tttagaagac
      481 atcgaacaaa tcgaaaatga tcgagtgatc catttccact ttgctaaacg aaacgagttg
      541 ggtgatctac aaaatatcat attgatcgtt gaattaatgg gacgtcatag tacgatcgtc
      601 ttagttaatc gagaaactgg aaaaatactg gatgcgatca aacacattgg aagctctcaa
      661 aacacttatc gttctttgct tcctggtgta gaatatgttg ctccgccaaa acaagaggtc
      721 ttgaacccat ttagctccga gaaagaaaaa atctttcagc ggctttccca aactgaacta
      781 gatccaaaag caatccaacg tcagtttcag ggaatcggtt tcgatatcgc tcaagagctg
      841 accaaaagat tactggaacg tcctaatgaa aagatggttg tatgggacga atttttctca
      901 gccatcagcc atcagcctat tcctactttt tacgaaacgg aaaataaaga ttttttcaca
      961 ccgattgctt atcaggtatt gtccgaacag gcatctgctg taacagctta tccaacactc
     1021 agtcaattat tagatagcta ttatcacgaa aaagcagaga aagatcgggc aaaacaacaa
     1081 ggcggagaac tgattcgaaa aatcgaaaat gaactaaaac gaaataaaaa taaattgaaa
     1141 aaacgtgaac aaacactcaa agaatccgaa aacgctgaga attaccgtag aaatggcgaa
     1201 ctacttacta cttttctgac acaagtacca cgcggcgcaa aagaagttgt tttacctaat
     1261 tattatgaag aagatcggcc aatcaaaata gcactagatc cggcattgac accaaatcaa
     1321 aatgcacaga aatatttcca tcgataccaa aagctgaaga acgctgttaa gctgattggc
     1381 gaacaaatcc aagaagccaa agatgaaatc caatatcttg aatctgtctt atctcaactg
     1441 gaaattgccg gcccaatgga tatcgaagca attaaagaag aattgacttc agaaggatac
     1501 ctgaaaaaga agaacttaaa aaaacaaaaa cgtaagaaac catctcagcc tgatcaatac
     1561 ttttcttctg acggaacgct cattcttgtt ggtaaaaaca atcttcaaaa tgaccagctg
     1621 acaatgaaga cagctaaaaa aaccgactat tggcttcatg caaaaaatat acccggttcg
     1681 cacgtcatca ttaaaagtga caagccctca gatgaaacaa ttacagaagc tgccgagtta
     1741 gctgcctatt tttcaaaata ccgatactct gcccaagtgc cggttgatct tgtacaggta
     1801 aagcatatcc gaaaaccaaa tggcgccaag cctggttatg ttatttatga aaaccaaaag
     1861 acgattatcg tgacaccaga agaagaaaaa attacagcta tgaaacaaaa caggtaatca
     1921 atgagtgttt ctagaatcat atttgcgtac tttcccacaa cagcaattag ttataaattg
     1981 tttgctacaa aaagaggagt tgtgacaaaa ttctgtcaca acccctcttt tttgatcaac
     2041 ggtgttcaat aaacagcgtt ctcatctgct tttcttagct ctgctggtcc agtaaccgag
     2101 taagctcttt gataaatctt tgagcgattt cactgagcgg acgtttcgcc gaataaatga
     2161 tataaaattc atttagttcg ccttctgcca aagggattaa ccgcaagtgc tcgcgaatcc
     2221 ctccaatcac aataccagat cctgaagcgt aagcatcagt ttcaagaacg atattcatca
     2281 aagtacctcg atcactcgta taaatcactg tttcttgatc agggatctcg ataaggtctt
     2341 cataaaaata cgggaaatta tttccatcct gtgtaaaacg aacttgagga taagataaca
     2401 attcttcttt ggtgatctct gcgtgttcag ctaatggatg tccttgcctt aaaaaaatat
     2461 gcgttttgaa ttttccaatg ctgcggccac ttagctctcc ttgttgtaaa tatcttctga
     2521 taccagcttg attatgctca ttcatataaa taatgccgat ctcactgtgc tgctctttga
     2581 cttcttcgat cactctcgct gttgtcgtct ccatgatccg aaattgtttg atctcgtctt
     2641 tgaattcctc gagtagctgt gcggtcaaag gacctaagaa atcataatgc tgggcagcga
     2701 tcgaaaaatt gtctctcttt tcactcgtta gatagtgagt ttctaatgta tcaagctgga
     2761 ccaatatttt ctcggcacga tttaagaagt cttgcccttc ttctgttaaa tatgcccctc
     2821 gattcgttcg agtaaacagc gtgatcccta actcggtttc taattctttg ataccagctg
     2881 aaagacttgg ctgggagaca aacattttct ttgccgcttc tctgaaacta ccattgtttg
     2941 ctacagcgac cacgtatttc atctgctgaa tattcattgc tcaaactcct cgaaataata
     3001 aatttctctt ccattcaagg gctggattgg tcggttgttc ggtcgagcat actgaccatc
     3061 gaaggggaga agaaactctt ggttcagttt tcctacatga tccattacaa accattggat
     3121 tggcccgctg gtaggcggag ttgtcaatga gccaatataa tggaaatggc ttttttgctc
     3181 cggaaagaac acatccggat ttacccaatg ctcatgtttt tctaagttcc aactttcccc
     3241 attttcttga ttatacagcc aaccttcctg taccattagc tggtacatga cacccacaac
     3301 tagattctct tttttgctgt tcatatgaac aaaatgaaat tcaatatctt cctgttttcc
     3361 gttgatgata tgttcagaag gcaagtggaa atgaatatct gtcaaataat agcgttcttt
     3421 cttgaactct acataactga gttggtcaaa aggtactaaa tgaatcgtgt ttttgaattc
     3481 cttgtcagta aaccgttgct tttgataatg aaaggaaagc gtgtctcctt ctattttggt
     3541 ttcttccccc ttggtcaaat gaataggcga ctgcaatgga aattccgcac ctctcgcaaa
     3601 ccaatcacat aaactcgccc aattttccgg acctttgtct ccagaatagc cccattcgac
     3661 atccatattc tttttcaact atacttcccc ctgcatctca tatcattttt atttttcttc
     3721 tttctttaca atcgttgaga cggtttctaa aaacgcctct tttccataaa attcctggtt
     3781 caatagttcc ggaaatatct ctttaaagaa atactgaaca gaaggccgat gttcaaagct
     3841 tttaatcgta gtcactgcct caatgaacat ctccttataa actttttccg ggttattttt
     3901 gatgatttca tcaaaagatt cataaagaag gtcaacctta tctgaaatgg ctaatagttc
     3961 tccttctacg gtttcatctt ttccttcgaa caaccgacgg cgataaactt cttggaactc
     4021 agctggaatc tcttggttga tgaattcgtc cgtcatcttt tcttcaactg tttgcagcat
     4081 ccctcttaac tctttgcttg cgtatttcac cggcgttttg atatcaccaa taaaacgctc
     4141 tgtgtagtca tgattcaatg atttttcata gagcagtttc caatcgatcg gttgtccatt
     4201 cgcttcttca atgtctccta gaacttgcgc aatagatgct acacgatacg aatgtgctgc
     4261 aactgtatgc tctgtatatt taaaaaatcc aggtgccctt gtgatcgtct ctaaattgtt
     4321 caatcctaaa ataaattcat tcaaccccat aaaagttacc tccctttgag atttcttttt
     4381 ccgaatatca tatcattaaa taactgatat tcgcaacatt tccgctcttt accatttttg
     4441 aaacagctcc cttaccaaaa aatagagaaa atctatatat aatagaaaaa agagaagaga
     4501 ttattctctt ctttctgcta accagtctta gaaaaatgaa ttttaaaaaa atcaaaaact
     4561 gatcacgata gaagtttctt ttcatacctg atgaaaagat gcttctcgca atcagttttt
     4621 ggatttttgc tattccaatt gatgattctg gagacacaaa tcggatttct ctatcttcat
     4681 caagcgcgct taaatctgcc aattttcagg atcttttttc cattctttta gcagagttaa
     4741 ttcttgctca tcgatataac gatcttctaa agctgcttca atcaatacag aataattggt
     4801 caacgtcata agagggattt cggctttttc gaaatttgct tttccttttg gcaactcata
     4861 cgtaaagata gccgcaacac caaggatatc cgcaccttct ctttttgctg cttcagctgc
     4921 ttccaagaca cttccgcctg tagaaatcaa atcctcgatc acaaccatct tctgaccttc
     4981 aaatatacgt ccttcgattt ggttaccttt cccatgatct ttcgccttac ttcgaatata
     5041 gaccattggc aaatccagta tttccgctac ccatgcggca tgagggattc ctgctgttgc
     5101 agttccagca atcacttgta cttcagggta agcttctttg attttgcttg ctaatccttt
     5161 tgcgatctct tttcggactt ttggatagct catcgtgatt cggttatcac agtaaattgg
     5221 gcttttgatt ccacttgccc atgtaaaagg attacttgga tttaaaaata ctgcttcaat
     5281 ttctaataag tcttttgcga tactttgttc aacattcatt ttaggttagc tccattctgt
     5341 ttgaatcgtt tgatatgctt tataaggatt agttgactga gtgattggtc gacctataac
     5401 gataaaagtt gagccgatct tttttgcctg agaaggtgtg actactcttg tctgatctcc
     5461 tgcgactgtt ccttctggtc ttatacccgg agttaagcaa acaaattcgt ctcctgccat
     5521 ttctttgatc gcttctactt cccaagcaga agaaaccaca ccatctaatc ctgctttttt
     5581 ggcacagcta gcataatgga tcacactttt ttctaaagaa acttcgatta gttgatcggc
     5641 atgcatttct tcttcacttg tagaagtaag ctgagtgata gctagaagta gcggacgttt
     5701 tccaccttct ggagtcccct cttctaatcc tcgcattgcc gcctccatca tacgaatacc
     5761 gccagcggca tgaacacagg tgagatcaac accaagcttt gccaatcccc gcattgcttt
     5821 ttcgaccgta ttcggaatat cgtgaagttt cagatctaaa aatacattat gaccagcctc
     5881 ttttagataa cgtacgattt caggtccttc ttggtagaac aattccattc cgattttcac
     5941 aaacaatttt tcttctttcg ggaaaaatcg aagaaagtct tctacctctt gcctagttga
     6001 aaaatctaat gcaataattg gtcgttggtc tagttgattc atcgttcacg ttcctctctc
     6061 acttctttga ttaatctttc cagcgattcg atgcctagtt cgctcattcg cacagggagc
     6121 tctttaatta gtttaggaca gatatacgga tcggtgaaat tcgcggtccc tatagctaca
     6181 gcacttgctc cagccataaa catctcaaga acatcatcca ctgtctgcac gccgcccatc
     6241 ccaatgattg gaaggctgga tatacttgct acttggtgaa tcaaacgaat agcgacaggc
     6301 ttgattgctg gtccggagag tccacctgtt tgattggcta aaatcggttt tcttgtctta
     6361 aggtcaatcc gcataccaag aagagtattg atcatcgtaa acccatctgc ccctccagct
     6421 tcgattgcct gagcgattgg aacgatgtct gttacatttg gggacaattt gacatagaca
     6481 ggaacagaag aaactttctt tactgcttgt gttaattgaa aagcaacatc agaatctgtt
     6541 ccaaaagcaa ttccgccatg tttgacattt gggcaagaaa tgttcagctc gattgctttc
     6601 acatttggtg cttcgccaat tttgctacaa acttctacat agtcttcttc acaagcacca
     6661 gcaacatttg cgatgatggg taactcattg tactcctcta attctggaag aagtttatgc
     6721 ataacaacgt ctagaccagg attttgcaaa ccaattgcat tcaacatgcc agatggtgtt
     6781 tccgccactc ttggcgtggg attgccaaag cgggcatttg gagtcgttgc cttgaccatg
     6841 atcgatccaa gctgattcaa atcatagtat tttccgtatt ctttgccaaa accgaaacag
     6901 ccgcttgccg gcatgatagg attttttaat tctaggccag gaagtttgac cgctaaagga
     6961 ttctcattca tataattacc tctcctattt taaaaatcgg tccttcgtca caaactttca
     7021 cactttttgt tcctgtttca tcttccggcg tatgacaaac gcaagcatag caagccccta
     7081 tcccacatgc catgcgggat tctaatgata actggacatt ttgatgcgaa gaaaacagct
     7141 gttccactgt cttcaataac ccgtttgaac cacatgcaaa aacagctgta ggttccgtct
     7201 tcgttcctaa caataaatta ccgacatttc cgtgcatacc gtatgttcca tcgtcagtag
     7261 cgattcttgt ttctcctaga cgattaaatt ctttttcata ataaacgact tcatgtgtgg
     7321 caaaacctaa aaaatgcgtt ggcttaattc ctttttcgac taactgtttt gataactcat
     7381 atagaggagg gacaccgatt cctccaccga ttataaatac ttcgtctcct gtatgaagat
     7441 tggacaaatc aaaaccattg cctaaaggac ccatgacatc taaatggtct ccggcagaaa
     7501 gagtagagaa gactctagtc ccctcacctt ccacccgata aatgatacgg caagtatttg
     7561 tttcgtggtc gatatgattc aagctaatcg gtcggcgtaa tagcaagtct gcacgcggca
     7621 cgagaatatg caagaactgt cctggttgct gcatttcttt tactagatca cctttcaatg
     7681 tcatttcaaa aatctttggt gccagctgtt tttgctgaac gatttccatc atttcttgtt
     7741 tcatctgtcc cctcctgcaa cggtttttcg tctttttgtc ttaacatgtg ctcgccgtta
     7801 aatagggtta gttgaaaaag cacgggattc catcactcga acgatcgcat ctgctgtgtc
     7861 taaagaagta aacaatggaa taccatgttc aactgcttct cttcggatga tgaaaccgtc
     7921 tttagaagca tttccccgat ctttatcaat cgtattgaca accacttgtg ttttaccatc
     7981 tcttattaag tcgactacat ttttttctgc tgcttcctca gaaattttcg ctactgtttc
     8041 aacgaccaga ccattttttt cgaaataagc agctgtatgt ttcgttccta tcagactata
     8101 gccaatttct gtaaaacgtt tagctaattc tagtgcttct tcttttgttt cgtccgcaat
     8161 cgtaaataat acggctccat attcaggcaa gtgtaatcca gaagcttcga atgccttgta
     8221 caaagctttg tctaattgtc tgtcagatcc cattacttct ccagtcgatt tcatttcagg
     8281 ccctaagtaa gtatcaactt tctgcaattt cgtaaaagaa aagacaggtg ctttaacatg
     8341 aacttctttt gtttctggat aaagtccgtt ttcataacca agtgtcttta gtttctctcc
     8401 caaaatagct ttcgtcgcaa tttgtgccat tggaattcct gtcaccttac ttaagaatgg
     8461 aacagtccga cttgctcgag gattgacttc gatcacataa acctcatttt catggatgac
     8521 aaactggata ttcatcatcc cgacacaatt caaacctaat gctaagcgga tcgtgtaatc
     8581 ttcgatcgtt ttcttgatcg attcagataa tgtttgaggt ggatacacag ccattgaatc
     8641 ccctgaatgg acaccagcac gctcaatatg ctccatgatc cctgggatga gtactgtttc
     8701 accgtcacaa atcgcatcca cttcgcattc accaccaagc aagtaacggt ccactagtac
     8761 tggatgttct ggagatgctt tcacagcatg ttccatgtaa tcttctaaat ctcgctggtt
     8821 ttcaacgatt tccatcgcac gtccgcctaa tacatagctt ggacgaacaa gaactggata
     8881 accaattctc gaagcgataa ccacggcttc ttctttactt gtagcagtat cccctaaagg
     8941 ttgaggaata tccaactctt tcagcgcttg ctcgaataaa tcacgatttt ccgcacgatc
     9001 caagtcttcg atcgttgtcc ctaagatttt tacaccagca gcagtcaaag gttcggctaa
     9061 gttgattgct gtctgaccac caaattgaac gattactcct atcggctgtt ctagctcgat
     9121 cacattcatc acatcttcga aggtcaatgg ctcaaaatat aatttatccg aaatagaaaa
     9181 gtcagtcgaa actgtttcag gattgctgtt catgatgatt gcttcgtatc ctgcctgctg
     9241 gattgctttg acagaatgca cagtcgcata gtcaaattca accccttgtc cgatacggat
     9301 aggaccagat cccagtacta gaattgaggg cttttctgat cgtgaacttt cattttcata
     9361 ttcataggaa ctataaaaat aaggtgtttt tgattcgaat tccgctgcac aagtgtcaac
     9421 catcttatat acaggaatca aatcattttc ttttctcatc tggcgaacgg cttcttcgtt
     9481 tatttcccac agctccgcca cttttttgtc tgtaaaaccg ttttgtttcg ctgttttcaa
     9541 tatcttgtca tccaacggct gctgtttcaa ctgctgttcg atttctacga tatgcaacag
     9601 tttatctaaa aagaataaat cgatttttgt caaatccgct aattcttcta tggtgtaccc
     9661 tcttctaata gcttcagcta cataaaacag gcgatcatct tgtgctcgaa ccactttttg
     9721 gattaggtca tcgtccgtga tgtctgccaa ctcaggaagt tcgatatggt gtgtaccgat
     9781 ttctaaagaa cgcacagctt tcaataaaga ctcttcgata ttccgaccga tagccatcac
     9841 ttcaccagtt gctttcatct gcgtgcctaa aagccgttct ccgttttcaa acttatcaaa
     9901 cggccagcga ggaattttag ctacaacata atctaatgca ggttcaaatt ctgcaaaagt
     9961 cgttcctgta acaggatttt tcatttcgtc taatgttaat ccaagcgcta ttttagcagc
    10021 tagcttagcg atcggatagc cagtcgcctt acttgctaac gcagatgaac gagaaacacg
    10081 cggattgact tcaatcacat aataattaaa actatgtgga tcaagagcga gttggacatt
    10141 acagcctccc tcgatcttaa gtgctcgaat aatcgaaaga gaagcatcac gaagcatttg
    10201 atattcatgg tcagacaatg tttgactagg ggcaaatacg atagaatcac ctgtatggat
    10261 acccaccggg tcaaaatttt ccatattgca gacaacgatt gcattatcag ccgaatctct
    10321 catcacttca tattcaattt ctttaaaacc tgcaatactt cgttcaatca aacattgggt
    10381 agcgggggaa agacttaatc cattttctga tacgatacgt aattcttctt cattttcaca
    10441 catacctcca ccagttccgc ctaaagtaaa ggctggacga acgatgacag gatagccgat
    10501 tttatttgca aaatctacag cttcttctac tgtattgacg atttcagact cggggattgg
    10561 ctgattcaat tctttcatca actgtttgaa tagatcacga tcttctgctt gatcgatcgc
    10621 agaaagcttt gttcctagaa gttctatgtt caattcttct aaaatacctg atgctgccag
    10681 ctccattgcc atattgaggc ctgtctgtcc gcctaaagtt ggaaggagag catctggacg
    10741 ctcttttcga agaatccttg atacaaattc aagtgtgatc ggttcgatat atacttgatc
    10801 tgccacttct ttatccgtca tgatcgtcgc aggattagaa tttactaaaa ccacttcata
    10861 tccttcttct tttaaggcaa ggcaggcttg tgttcctgcg tagtcaaatt ctgcggcttg
    10921 accaatgatg atcggaccag atccaatcac cataattttc ttgatatctg tacgtttcgg
    10981 catctgttta ctgctccttt cctgcgtcca tcatttccat aaattcatca aataaatgta
    11041 gtgcatcatg cggaccaggg gcagcatctg gatgaaactg cacactgaaa gcgggataat
    11101 ctctgtgttt gactccttct actgttccgt catttacttc tacatgagtt acgatgagtt
    11161 tttctggatc aatcgtggct tcatcgactg catagccatg attttgagaa gtaaaatcaa
    11221 ttcttccggt cgctacttct cgtaccgggt gatttaatcc tcgatgtccg aatttcattt
    11281 tataggtatc tgcaccgttg gctaaagcaa ataactggtg accgagacaa ataccaaaaa
    11341 tcggtacttt tccttggatt tttttgatca tttcgattgc ttctggcaca tcttttggat
    11401 caccaggtcc attcgtcagc atgactccgt ctggtgatag cgctaaaatc gtctcggcat
    11461 ctgtgttata gggaagtact gtgaggttac agtttctttt actcaattct cgtaaaatac
    11521 tatgtttcaa gccgaagtca atgacaatga ctttccgacc tgttcctgga cttggataag
    11581 gtttgttagt tgagacttgc tgcacttgat tggttgctag aacggttgct ttcagctgat
    11641 caaatgcatg ttcgaaggaa tctccagcat caactatact agctttcatc gttccgactt
    11701 cacggatttt gcgagtaagg gcgcgcgtat cgatacctga tattccagga attccttttt
    11761 gtttcaaaaa tgaatctagc gtcatttggt ttcgccagtt tgaagctaca cgtgcatgtt
    11821 cttttactac tactcctttg catgttggag aaatcgattc ataatcatct ctatttaccc
    11881 cataattacc caccatagga taggtaaatg taatgatttg accgttaaaa ctttgatcag
    11941 tgatagtttc ttgataacca gtcatacttg ttgtaaaaac gatttctccg aagacactag
    12001 catctgcacc aaaagccttt ccttcgaaaa ctgttccatc ttctaatata agcaaacgtt
    12061 ccatactttt attctccctt ctgccacaca agttttccgt tgacaaaagt catttgtgtt
    12121 tcgcctttta ctttccagcc gataaaaggt gtattctccc cttttgataa aaagtcttct
    12181 ttatcgatcg tgcaagtttg tgtaatatca aaaactgcta catcagcggg ggcaccaacc
    12241 gttagcgttc ctgcattcaa accaaaaatc tcagccggtt taactgccaa ccaattgatc
    12301 acttgttcca acgtgaatcg accagtttct acaaaatgag tatagatcag ttgaaacgct
    12361 gtttcgcttc ctacgatacc aaatggtgat ttcatgaaac tttggctttt ttcttctaat
    12421 ccatgggggg catggtctgt agcgatgcaa tcgatcgtac cgtcaagcaa gccttcgatc
    12481 aaagcttctc tgtcctcccg tcctcttagc ggcgggttca ttttccaaaa gccgaaatct
    12541 tcgggaatat cttcgtcgat caaaataaga tgatggggtg aaacttctgc tgttacgtgg
    12601 attcctgctt tttttgcgtc tctgattaca cggacacttt ctttagtaga aacatggcaa
    12661 acatggtaat gaacaccagt tgcttcagct agcaacagat ctctagcaat ttgagaagac
    12721 tcggttacgc ttaagatccc gggaagacca agttcttctg ccttcttgcc agcgtgcata
    12781 acgccgccaa ataataaaga ttcatcttct gtatgggcta ctagggcttt attgtttttt
    12841 gcagcttctt tcatagccag atacatcgtc ccagctgttt ggacacctac accgtcattt
    12901 gtgaaagcaa aagcaccttc ttcaatcagt gcttcttgat caactaattt ctcacttcgc
    12961 agattttctg tgattggcgc atactgcaat accttcacaa ctgcatcttt tctaatgatg
    13021 tcatatactt gtctcagctt ttctgccgta tcaggtacag gatttaaatt cggcatcgcg
    13081 catacagtag tgaaaccgcc acgagcagct gagcgagtcc cagcttcgat cgtctccttg
    13141 tatgtaaatc ctggctctct caaatgaaca tggacgtcca ctaaaccagg tgtgatcaat
    13201 tgtccctttg catcaaacac ttcatcaaat tcggcttctg agaatcctgt accgattgct
    13261 ttgattcgcc catcttctat ccaaatttct gctggtgtcg tcatattttt tcgtgtattg
    13321 atctgaccgt ttttaatgag tgttttcatt ttttcatcct cctataatta cgcttttcca
    13381 tttagcactg cttctaagat tgccattctg acgaatactc cattgctcat ttgctgaacg
    13441 atccgcgact gtaatccttc cactaatgaa tcagctaatt ctacatcacg gttaactggt
    13501 gctgggtgca taatgattgc atgtttttgc aacttcttcg cacgctcaac tgttaaccca
    13561 tgttcctgat ggtagccttc ttttgagaaa ctttcttttc catcatggcg ttcgtgttgc
    13621 acccgaagca tcatcatgac atcaacttga tctagtagtt catccaatgg catataatgt
    13681 ccgtacactt cgaattcatc gtcgtaccat tctctaggac ctgagaagaa tacttgtgcg
    13741 cctagacgtt ttaacatctg catattggat ttagctacgc gagagtgagt gatatcacct
    13801 acgatggcaa ctttcagacc ttcaaaatga ccaaattctt cataaatggt catcaaatca
    13861 agcaagcatt gggttggatg ctgtccgctt ccgtctcctc cattgataat cgaacactgg
    13921 attgttttac tttgtatcag ttcgtcataa tagttctcat caccatgacg gataaccgct
    13981 acgtctacgc ctaatgctga catcgtcagt actgtatcgt atagtgtttc ccctttttgg
    14041 acagagcttg tgcttgcttc aaattcaatc acttctagcc ctagtttttt ctccgctaca
    14101 tcgaagcttt tatgtgtgcg agtactattt tcgaaaaaga ggtttgtagc aaagtactgg
    14161 cgcttttgcg gagtccaatt agcaccttgc ttgaattcct gccctctgcg gatcaagccc
    14221 ataacttctt gatcagtaag tgcctcaacg gtcaataaat gctttaagct gatacgttct
    14281 gatttgatga tcataatgtt tcctccttag ttttcttcgc ttcgtgaagt ttctggtaag
    14341 ataagattta aaatgatgcc aagtacagtt gctaatgcca ttgccgacaa tgtaaatgtg
    14401 cctacttcaa agacgagtcc gccaattcct actactaaga taactgaagc aatcaacaga
    14461 tttttctttt tatcaaaatc gattttgttc tcgattaaaa tcttcattcc acttgcggca
    14521 attactccaa ataagataaa actgattcct gagatgactg gtccagggat acttaaaatc
    14581 aaagcgctta attttcctac gaatcctaaa atgactgcaa atactgcagc tccaccgatc
    14641 acaaacacgc tatggacacg tgtgatagcc agaacaccaa tgttttcacc gtaacttgtt
    14701 acaggaggac ctccgacaaa ggctgccacg atttgtgcta atccatcacc tgtcaacgtc
    14761 ttatgaagac ccggttcttc aaagaaatta cgtttagtta gtttattcaa taccataaga
    14821 tggccgatat gctctgtcat cgtcacaaaa gcgatgggtg ccattgttgt gattgctcca
    14881 agatatagct ttggttcata ttgtacaaag ggaatctcaa agttaggaac agcaaaccag
    14941 gcagcgtcta cgattggctt agtatccacg attccaaaca gcaaggctac gatgtatccg
    15001 ctaataatac ctagtaaaat cggaatcaat ccgagaaaac ctcttagcca catgttgaag
    15061 aaaattgtta atcctaatgt gatcagcgct acagcaatat acttgaaatc atacgtgccc
    15121 tgattataca tagcatttgt tgctgcattt gccgcaagtc ccaacccgat aaccatgatg
    15181 actggaccga cgacgattgg aggcaagatt ttatccagcc atgctgaacc gatctttttg
    15241 atgatcagcg caacaatcaa atagactaat ccagttgtga ctgctccttg tgcaatcgct
    15301 ggataaccat ctgttttcat caaaagctgc atcgctgcaa tgaaagcgaa gctgcttcct
    15361 aagtacgcag gtatctttcc tttagtagta aataagtaca ccaatgtacc tagtcctgaa
    15421 ctgactagcg caatacctgg atcgatacct actaagatgg ggactaacac ggttgctccg
    15481 aacatcgtga acaagtgttg taaacttaat cctacccaat gtcctggggt tgggcgatcg
    15541 tggatatcta acaccacgtc atcattatga aatccttttt tctccgacat gtttctctct
    15601 cctctaatct tcctctttca aaatcatgat gcgatcttgt ccatcaagct cttgcatctc
    15661 tactaaaatc tcttccgccc gagatgttgg aatgttcttc ccaacataat ctgcacggat
    15721 tggcaattct cgatgcccac gatcgaccag gactgctaat gaaatttttc ttggtctgcc
    15781 aaaatccatt actgcgtcca ttgctgctcg aatcgttcgt cctgtataca aaacatcatc
    15841 aatcaaaata acttcttttc cttctaagga aactgggatg tcagaagaat gaagttccgg
    15901 ttcttctgga ttttcttttt tgtcatctcg gtataaagtg atatccagtt ctcctacagg
    15961 aatatcgata tcttctagct gcttcaggcg ttcggcaatt cttgatgcaa tgtagattcc
    16021 tcgtgttttg atacctacca atacgatatc ttggatacta tggtttctct cgataatctc
    16081 ataagtaatt cgagtcaatg ctcgcttcat ggttacctga tctactactt cttttgcttg
    16141 cattttcatt ccccctactt ttttgcataa aaaaactcct gccctaagag gacaggagga
    16201 atgtttagac tgagcgtata ctgatacact gatagctata ctatgtcaat gagcaatcta
    16261 cttatggcgc accatacgta tccttgctcc cacccttctt agcctcacgg gactaaactt
    16321 aaaggaattc tattaagttt tatgctttcg ttgttaatta ctataccgtt atctaaatta
    16381 aaatgcaatg ttttttctta atttttctaa agttttttca aaaacttctg gaagaggtgc
    16441 ttcaaagatc atcttttcgt ttgtagtcgg gtgcgtaaaa cctaacattt gcgcatggag
    16501 aaactgacca ttgcctttca aagttttctt cggtccgtat actggatctc cagctaacgg
    16561 ataaccgata tacttcatat gcacacggat ctggtgtgtt cttccggttt ctagttttaa
    16621 ttcaaccaga gtaaatcctt cgaaacgttc caatacagta aaatgtgtga cagccgactt
    16681 cccatcttct cttatcgcct gcattttccg gttttccttc gaacggccga ttggcgcatt
    16741 gatggttcct ttttcgtgag gaatctctcc atggactaaa gcaacatatt tgcgcaaaga
    16801 ggtcttatct tttaattgct gagctaatga ttcgtgtgcc tggtcatttt tggcaaccat
    16861 tagcagtccg gaagtatctt tgtcgattcg gtgaacaata cctggacgaa taacgccgtt
    16921 gatcgtagaa agatccttga tatggtacat cagcgcattg accattgtgc catgcgtatg
    16981 acctgctgaa ggatgaacga ccattccttg aggtttgttt atcaccgcta catcttcatc
    17041 ttcgtaaacg atcgtcaacg ggatattttc tgggaccaca tctaatgttt ctggttcggg
    17101 gatcaagaca gtaatctcgt ctccttgttt tactttgtaa tttgctttga ctggctgtcc
    17161 attgacagaa accgctccat ttttcagcca cagctgaatt tgcgaacgac tgtaatcggt
    17221 gaataaatta gtcaatactt tatcgatcct accagattca tggtgaatcg tcgttttaag
    17281 ttctgtcatt tttttctttg atccttttca tctaaaataa tataaattaa catacaaatc
    17341 actccacata caagtgtcac atctgctacg ttaaaaatcg gaaagctcat aaaatctgtc
    17401 tggaacatat ctactacata gccaagacga atacggtcga taaaattgcc aatcgcacca
    17461 gctagaatca aactcaatcc aatcatgaac cagcgattac ctttttcacg atttttaatc
    17521 atcaatgtga gaatcacaac gactgcaatg agtgtcacta tagtaaagaa ccacatcttc
    17581 ccttctagca tactccatgc ggctccagtg tttcgaatat gattcagtga caaaatacct
    17641 ggtataagat ttttcgtctc tccaagcgct agattatcag tgatccacca tttgaccaac
    17701 tgatccactc ctactagcaa agcgctgaat atccaataaa taattaacaa aaaatcaatc
    17761 cttttctatt tgtttataaa tatcacgagg tcgtacgatc ccgagcagat taccatcggg
    17821 attcccatct ttagtgataa gtacggcttc caaagtccct tgctcacgaa acatcgcttc
    17881 aacctcgtaa atatgtgttt tagctggaac aaaatgataa tttgtgtact ttccgttttg
    17941 gatgatcaaa tccaacgctc gtttatttgt taaatcaacc ggaccttttt ggctttcttt
    18001 tgctagccaa aacccgatat tacgtaaagt aatcaatgct acaaattttc ctttattgta
    18061 taacggaaat tgtgaatacc ttttttctgc tactatcttt aacagttcaa gtaacggaag
    18121 atcttgttca aaacctgtta cacgttttgc aaatcgaggc agcacggttt caggctgaat
    18181 cagttcttgt tcgatcagct ggattcgctt gactgtccaa tcatttggct ctgcaatgac
    18241 aaaatcgact gcgatccgat catggacgat cgcattcctt aactgagcaa gctgtaaaag
    18301 atcatcttcg tattttctga tcattaattg ttttctttga gctaggcgtc tgacaagttc
    18361 ggtaaatccc atatttcgtg catttcccat ctcgtctctc atccattttt cgattcgatt
    18421 gaaactgatt aaaaaaagat cagagtttgc cataattttc ctccttgatc aaatatgccc
    18481 ggttttttat atagaaaaag aacgcaatca acacaataac tgcaaaaaaa ctgaaaaata
    18541 aattttgtag gaaaacggcc cccaacccag ttgctagaaa taaaaacgga tagatttgaa
    18601 gaatcaagta attcattgtg atcacataag caggctttgc ttctttcact gtataaacgg
    18661 ggctttttag ccaataaaag gtgacctgca tcgtaaaggg cacttccaga tcaagcgtta
    18721 tcttttgttc atgactgaga acactgcgtt tttcattgtt gattcttagc tgaatcgagc
    18781 tacccatacc gtaaaaaccg gtttttctta taaatgtcgt tttcatcata ccctcctatt
    18841 atgctttagt atagctcaaa atacaataaa gaaaaagaat gatcctagtg taaacaaaat
    18901 taattcccta tcgtctaaaa aaagaagctg tagtcaaaat acagctcctt cgatggtaga
    18961 ttataatcaa gcttaaaaca atcccaacac ttctccgtca tttgtcacgt ccatattcaa
    19021 tgcagcaggt tttttaggca aaccaggcat cgtcataaca tctccagtca aggctactaa
    19081 aaatcctgcc cctaattttg ggatgatttc gcggatcgtg atggtgaaat cttccggtgc
    19141 acctagcagt gaaggatcat cagaaaatga atactgtgtt tttgccatac aaatcggcag
    19201 tttgtcccat ccattcttcg taaactcttc tatttgtttt tgcgcttttt tgctgaaaac
    19261 aacaccattt cctccgtaaa ttttcttgac gattgtttct gtcttttctt gtatcgtttc
    19321 ttctaggcgg tataatggct tgtaattagc ttcttttcta tcaatcaatc gtactacagc
    19381 ttttgctaaa tcaacgccgc cttctgctcc atctgcccat acactcgctc tcttggcaag
    19441 aatcccctga ttttcacaca agtgctccaa cagtgtgagt tcattgtctg tatctgttac
    19501 gaattcattg attgcgacga tcacaggtag ctgatattgt tccatattac gtatatggcg
    19561 ctttaaattt gcaaaaccta ctttcaaagc atcaagattc tcatttttaa gttcatcttt
    19621 tttcattccg ccatgcattt tcaatgcacg gacagtggcg acaatcacca cagcatccgg
    19681 cgcttttttc aagttaggta ctttgatatc taaaaatttc tctccgccaa gatctgcacc
    19741 aaatcctgcc tctgtgacaa cgtaatctgc taatctcaat gctgtcttgg tagctaaaat
    19801 actattgcag ccatgagcga tattggcaaa cggtcccccg tggacaaaag ctggtgttcc
    19861 gtaaattgtt tgtacaagat tcggcttgat tgcatctttc agcagtaatg ccagagcgcc
    19921 ttctgctttc aagtctccag cagtcacagg ctgacggtca aatgtatagc cgacaacgat
    19981 ccttgcaaga cgagccttta aatcttcaag gtctgccgca agacacaaga tagccatgat
    20041 ttcgctggca actgtgatat caaacccatc ttctcttgga acaccttgga ttggcccacc
    20101 caagccaaca attactttac gcagttcacg atcattgaga tcgactaccc gtttccaaat
    20161 cactcggcgg gcatcgatgt tcaattcatt tccttgatga atatgattat ctaaaagagc
    20221 agataatgca ttattagctg ttgtgatagc atgcatgtca ccagtaaaat gcaagttgat
    20281 atcttccata ggcaatacct gtgcatatcc tccgcctgca gcaccacctt tgattcccat
    20341 cacaggacca agtgagggtt ctcgcaatgc aataacagat tttttattga ttttattgag
    20401 cgcgtctcct aaccctacag tgatcgttga ctttccttcg ccagctggtg ttggattgat
    20461 ggaagtaact aagatcaaat gcccctcagc gttatccttt gatcgattga ttccagaaaa
    20521 attgattttc gccttatatt tcccatacag ttccaaatcg tcttccttaa gatctaattt
    20581 actagccact tcaacaatcg gttctagctc tgtttcttgt gaaatttgca gatcattctt
    20641 catttgatta ccccttttca cgaactttat tcaacaaaat atcttctttg ttaagatatg
    20701 ccaattatac ctttttttat tcagaaaaaa aacataaaat tacaggtttt taatttattt
    20761 gacgtactta taaatactct ttataatgaa ggctatgatc agactttaga aaggtcgtga
    20821 gtgctttgac cacaggaatt gtaaaatggt ttgacaacaa aaaaggatac ggctttatca
    20881 gttatgatga cacagaagaa atttttgtac attttactgc gattgaagaa gagggattca
    20941 aaactttgga agaaaatcaa gtggtcgaat ttgagatcat cgaagggaat cgaggaacac
    21001 aagctgccca tgtcaagaaa ggcaacgttc agactaccga tactagtatg catgaataga
    21061 taaacaaata gagtgagacg ctaaacggga tgaaccgtac gtctcactct tttcttttcc
    21121 tgtaaaaatt cgttttttta ggttctgatt tttaaaatta gctttttctt tttttcgtat
    21181 aaggataagg aattgattct gatttgggtc cgtttttttc aaaatgatca aaaaggacta
    21241 cttgatcttt ttttcgcatc atgaaaacag atggggcttt ctcatcttgc aacatcattt
    21301 gcaccccagt attagttggc gtcaaagatc gaatctgtcc gacagatatt tctcggtctt
    21361 tccaaaaagc agcataacgt ataatcacag agtttgattt taaaataaaa gaacgccgca
    21421 aacctagaaa tagaaaaaca agaaaaatac ctaaaacaaa attgcttttc caataaggac
    21481 ctgtgttttc taaagaaaga atcagactaa aaaacagaat gatcaaggtc aatgaccaat
    21541 agatgatcgt atgtgccagt tcaggctgcc atcgaatatt ttttttcatt ctctaacctc
    21601 tcaccgattc agtaatgata taatcttacc ataacattag tttgaacaaa aaggagaatc
    21661 tctttttatg ttaaaagtat acattgatgc ctctacaaaa ggcaaccctg gtcctagtgg
    21721 aggtggaatc ttgattgtct atcaaggtaa acaagaacag ctatctatcc cgctttctat
    21781 agggacaaac catcaagcag agttcgaagt ctttttaaaa actttagaat ttctaaagaa
    21841 aaacgactta cagaatgaaa cgattttctg tttttcagac agcaaaacac tagtttctac
    21901 ggtcgacaaa aacgcaacga agaacgagct cttccttccc taccttcaaa gaatccaaga
    21961 acttctatcc gaattttctt tattgatcct gcaatggttg cccgaaaaca aaaataaagg
    22021 agcagataat cttgcacgtc aggctctcca taaacaatta aaagaaaaaa agactcttta
    22081 aattaaaaa
//
LOCUS       DAZKMB010000039        21993 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000039 DAZKMB010000000
VERSION     DAZKMB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.039"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1432)
                     /locus_tag="ACG8V3_001306"
     CDS             complement(41..1432)
                     /locus_tag="ACG8V3_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0299854.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            1689..5078
                     /locus_tag="ACG8V3_001307"
     CDS             1689..5078
                     /locus_tag="ACG8V3_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0299855.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            5081..6502
                     /locus_tag="ACG8V3_001308"
     CDS             5081..6502
                     /locus_tag="ACG8V3_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0299856.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            6499..8376
                     /locus_tag="ACG8V3_001309"
     CDS             6499..8376
                     /locus_tag="ACG8V3_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0299857.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            8468..9310
                     /locus_tag="ACG8V3_001310"
     CDS             8468..9310
                     /locus_tag="ACG8V3_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0299858.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(9462..10337)
                     /locus_tag="ACG8V3_001311"
     CDS             complement(9462..10337)
                     /locus_tag="ACG8V3_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0299859.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            10544..11574
                     /locus_tag="ACG8V3_001312"
                     /pseudo
     CDS             10544..11574
                     /locus_tag="ACG8V3_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11663..11968
                     /locus_tag="ACG8V3_001313"
     CDS             11663..11968
                     /locus_tag="ACG8V3_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0299860.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            12147..14162
                     /locus_tag="ACG8V3_001314"
     CDS             12147..14162
                     /locus_tag="ACG8V3_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0299861.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            14164..14448
                     /locus_tag="ACG8V3_001315"
     CDS             14164..14448
                     /locus_tag="ACG8V3_001315"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0299862.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            14474..15847
                     /locus_tag="ACG8V3_001316"
     CDS             14474..15847
                     /locus_tag="ACG8V3_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0299863.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15930..17945)
                     /locus_tag="ACG8V3_001317"
     CDS             complement(15930..17945)
                     /locus_tag="ACG8V3_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0299864.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17967..19181)
                     /locus_tag="ACG8V3_001318"
     CDS             complement(17967..19181)
                     /locus_tag="ACG8V3_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0299865.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            19401..20345
                     /locus_tag="ACG8V3_001319"
     CDS             19401..20345
                     /locus_tag="ACG8V3_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0299866.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            20641..21585
                     /locus_tag="ACG8V3_001320"
     CDS             20641..21585
                     /locus_tag="ACG8V3_001320"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0299867.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7487 a         4010 c         4142 g         6354 t
ORIGIN      
        1 ttttatcagg gtggtttata catagctgtt ttttgattat tcataactcg aataagcgga
       61 tgagttcttc ttcatttgcg aatgacacac tatcatcaat caatatatga ggaacatggg
      121 cattcaaaat gggttgattc gtaacgatca gatcatactc gagaagattg tatttcgaca
      181 gctgatcttc agtaatagca gttgtctcag agtgaatttt atgcacagca agaatatcac
      241 tgatatattc ttcaatatat tgactatgtc gaagacttaa gtcgctgata atcagtattt
      301 ttttatcaga agtataatgg tgaatttcag gattaccgat aagcatccaa aagatatagc
      361 tagataaata ataaatcaga ttctgattca gtagagtgga ataaaattga agttctgcac
      421 gaaatccagc atatagacgt atctggcggt gttgcatctg ctgtgcaaag taagttgcac
      481 ggatcgtcaa atgagtcgta tcgtatggga aaatacggga gaaaaaataa atttgcgtta
      541 ataattgaac aacatattct tcgttttttt ctttgcaatc agcggatgaa aacgaacagt
      601 aggacatatt ttttcgtatc tgctcactga tttcattttt ttctttctcc gaaaattctg
      661 aaaaaatcgt attgacaaaa tagttcacta cttttatata gctgctgtac ttcaattttc
      721 ctaagagatt tttcaagatt aagaagtctt ttttatcgat atttatcaac tgtacataag
      781 agtctccagc tttccattca aatggagttc cttgggattc tctcatgaga tgcacaacaa
      841 aatacataat aaagaaagat tgctcatatg ggctttcttc ctgaaacaaa ttcatctgac
      901 aattattctt gaccataatt tctttgatct tctttttttc gattggaaat ggcaacgtgc
      961 gtcttccgta gtaacttaaa aagaaaaagg taacaaagat acgcaactgg agctcacttt
     1021 taaggctagt taactgaaaa ccttggtcaa cgatcacttt taataaatag ctttctaaaa
     1081 tttgattgca ttgtttcaac ttacgtgtaa aagaagaccg gctgatggaa attttttcac
     1141 aataatactc aatcgtttta cccggattga caataatctc atttaataaa cgactgtttg
     1201 tagaactttg cgacatttcc atcagaacat ttaaataagc tggtacattc aaatgttcaa
     1261 gacgataccc caatttttgt gaagattgaa tcgttacatc cgttccccag cggattttga
     1321 tctcttcgat ataccttaca aatgtagatt ctgaactacc aattcgtttg gcaagttctt
     1381 ttttagtcat ccacttgtca gaaatggaca gaattcgaat gattttttcc atctttctat
     1441 agtttaattc caacaacctg atcatatgtt tttctccctc aacccttttc tttcaatgaa
     1501 acgctcatta atcgaaggaa tcaacttcat aaatgaaacc aattggtatg aaaattggtg
     1561 tgattccctt tagcgccctt cgtataataa ttatcatatt aaaataaata aaattatgta
     1621 acgcctgttt attttaataa ttttcactat tcacgtcaaa acagaaaaaa agaaaaagga
     1681 ggggaaaaat gacgaccaca gggaagaaac tgaaagttat tttcatgctg ataatattga
     1741 gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac tacagatgat ccaacagttt
     1801 tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg aaaaaaagca ctgaacatca
     1861 agctaaatgc gaataacacc agtgctgaaa agatagaaaa agaaattggt ctagtcgaaa
     1921 attacttaag tgatgtggaa agaaaagaag gagatggcta tgcttatcag gtaaatagcg
     1981 ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac tatcgatctg agttttccaa
     2041 tcgatccagc actttaccac agccaggcaa acaagctgat cgtcgataat aaagaatatg
     2101 acattattga tgagacagaa aataagaaag atacagatgt gtcagtacca aagccagacg
     2161 aaatagaaga agaatcatca aaagaaaacg aaaattctgt cagcccattt acattgccta
     2221 cattatcctt gccagctgtg agtgtgccat ctaatcaaac gattcctaca gaatatacaa
     2281 cagatgatca gggcacttat cctaaagcca gttggcaacc tacaggaaat acaaatgttc
     2341 ttgatcatca aggcaataaa aacggaacaa atcaatggga tggtataaat tcttggaatg
     2401 gagatcctaa tgatcggacc cattcgtata tcgaatatgg aggaaccggt aatcaagcag
     2461 actatgcgat acgaaagtat gcaaaggaaa caagtacacc cggattgttt gatgtttatt
     2521 tgaatgctcg tggaaatgta caaaaagata tcacgcctct tgatctcgta ttggtcgtag
     2581 actggtcagg aagtatgaac gacaataatc ggatcggtga agtaaagatt ggtgtcgatc
     2641 gttttgtcga tactttagca gatagcggta tcacagacaa aatcaatatg ggatatgtcg
     2701 gctactcaag cgaaggatat agctacagta acggtgcagt acagatgggt tcatttgatt
     2761 cagtgaaaaa tcaagtaaaa tccattacac cttcacggac aaatggtggt acttttacac
     2821 aaaaagcact aagagatgca ggaagcatgc tatccgttcc aaatggacat aaaaaagtga
     2881 tcgttttgct gacggatggt gtaccaacat tttcctataa agtacagcgg gtacacgcac
     2941 aatcaagcag caattattac ggaactcagt tttctaatac gcaagatcgg ccgggaaata
     3001 cttctctaat ctcaagaatc tatgatgcac ctgaccaaaa caatctatcc agaagaatcg
     3061 acagtacgtt tatcgcaacc atcggagaag cgatggcact caaagaacga ggaatcgaaa
     3121 tacatggtct tggcatccaa cttcaaagcg atccggcagc tggtctctca aaagcagaag
     3181 tagagtctcg tatgcgacaa atggtttcat cagatgaaaa aggcgatctt tactatgaat
     3241 cagctgatca tgcaacagat atctctgaat acctagccaa aaaagctgta cagatctcag
     3301 caactgtaag caatggacaa ataaatgatc caatcgcaga accattcatt tatcagcctg
     3361 gtacactttc agtcaagagt gtggggacaa gtcctacaac ggtcactcca tctatttcca
     3421 tagaaggaaa taccatcaag agcaatcaga tctatttagg aaaagaccaa gaaatccaaa
     3481 tccattacca agtgagaatc caaacagaaa atgaggactt ccatccaaat ttctggtatc
     3541 aaatgaacgg caggacaact ttccagccaa acattgatac caatgaatta gctgaattcg
     3601 gtataccatc tgctaaagct cccggagtca gtcttcacat caaaaagtta tgggaagaat
     3661 ttgacaacaa tctagctgat cgtccagatc aagttacttt tgagattcaa cgggaacata
     3721 cgacaaatgc tgcagcttgg aaaaacggat atattcgaat cattaaacca gctaaagata
     3781 caacaaatac gtgggaacgt gcagacattg acaaattatc tgcaaatagc ggagaaagtt
     3841 atcaagagat attatcacta cctcaataca ataatcaagg tcaagcattc agttaccaaa
     3901 caatcaaaga attacctgta ccaggatacg attctcaaca aatagatgca atgacatgga
     3961 aaaatactaa acaattcaca ccgttaaact tgaaaataac gaaaaattcc tctacaggtg
     4021 aaaaggatct tattggcgct gttttcaaat taacaggaga ttctattgat actttactaa
     4081 cagatcatgg cgacggaacc tattctcttc cagaaaatgt caaattgcaa aaagaaatga
     4141 cctatacgct gacagaaaca aaagctccag aagggcatgg attaagcaaa aagactactt
     4201 gggaaatcaa gatcgcttct gatggtacgg taaccattga tggaaaaaca gtcactactt
     4261 ccgatgatac gatccagttg actattgaaa atccttttgt tgaagttcct gtagcagtac
     4321 gtaagtatgc gatgcaaggg acggacaaag agataaatct taaaggagca gcattttccc
     4381 tacagaaaaa agaagcaaat ggtacttatc agccaattga cagccaaaca acgaatgaaa
     4441 aaggtcttgc cagttttgat tcactcacac ctggtaaata tcgagtcgtt gaaacagctg
     4501 gtcctgccgg atatgatact tcgccgggaa attatgaatt ccaaatcgat aaatatggaa
     4561 aaatcattta cacgggaaaa aataccgaga tgacaaataa tgtatggacg ctcactcatc
     4621 aaaatcgact aaaagcgttt gatctaacgg tacacaaaaa agaagacaac ggacagacat
     4681 taaaaggagc aaaattcaga ctgcagggac cagaaatgga cttagaatcg ccaaaagatg
     4741 gacaagaaac agataccttt ctattcgaaa atttaaaacc tggaacttat acgctgaccg
     4801 aaacttttac accagaagga taccaaggtc taaaagagcc agttactata gttatacacg
     4861 aagatgggtc aattcaagtg gatggacaag atcatgaatc tgttctgtca ccaggagcca
     4921 aaaacaacca gatttcttta gacatcacga atcaggcaaa agtaccatta cctgaaacgg
     4981 gaggaattgg ccgtttagga atctatctag tagggatgat tggttgtgcg ttttctattt
     5041 ggtatctttt tttgaaaaaa gaaagagggg gcagctaaat atgaaaaaac ttggttggct
     5101 tagtatgtgt ctcttcttgt tactatttaa accagctttt actcaggtag caacagaaac
     5161 agaaacagaa atggttcaga ttactttaca caaattgctt ttcccaaacg ggcaactgcc
     5221 gaaaaatcat ccaaatgacg gacaagaaaa agctttatta caaacgtatc gaggattaaa
     5281 tggtgtcaca ttccaagttt atgatgtcac agattctttt taccatctac gggaaaaggg
     5341 caaaacggta gaagaagcac aagcagagat cgcaaaaaac ggtgcgtctt ccggtatgtt
     5401 taccgcagaa gcaacaacta caactcttaa caacgaagat ggtatcgctt ctttttctct
     5461 ggccgctaaa gatcaagaaa aaagagataa agcgtatctt ttcattgaat ccaaagtacc
     5521 agaagtcgtc aaagaaaagg cagagaatat ggtagttgtt cttcctgtac atggacaaaa
     5581 caatcaaaaa ctttcaacta tccatttgta tcctaaaaat gaagaaaacg actaccctga
     5641 tccacctttt gagaaggtat tagaagagcc tagaaatgat tttacgattg gtgaaaaaat
     5701 cacttattcc ttgcatacga caattcctgt aaatatcctt gactatcaaa agttcgaatt
     5761 gtcagatagt gcggatgaag cattaacgtt tttacctaat agtttaacga tttcatcgaa
     5821 tggagaaaag ctgacagaag gctttgtcat acacaagaaa cctcacggat ttgatgtttt
     5881 attttcgatc ccttcgttgg aaaaatatgc tggaaaaaaa ctgaccattt cttatcagat
     5941 gcagctaagc agtacagcac aggcgaacaa ggaaatcaac aacaacggaa cactggattt
     6001 tggttttggt gtcagtacaa agaaagtctc tgtatataca gggagtaagc aatttgtcaa
     6061 aatcgagaca aataaaccag ataaacgatt agctggcgca gtattcctta ttaaaaacaa
     6121 agcaggaaat tacctccagc aaacagccaa cggatacaag tggacaaaga acgaatcaga
     6181 tgcgcttcac ctgatttccg ataaaaatgg cgctttttca atttccgggt tgaaaacagg
     6241 aagttatcga ttaaaagaga tcgaagcacc ttctggttat attttaagtg aaacagaaat
     6301 tccgtttacc atttcaactt ttctttctga ggataaagag gcggacagta tattgaaagt
     6361 agtcaataaa aaagaaaata gccgtccatt tcttccaaaa acaaacgaaa cgaaaaatac
     6421 acttttaggc gttgttggta tggtattcgc aagctttgca atctggttgt ttatcaaaaa
     6481 aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa acgttatgtt aggagtcctt
     6541 ttccttattt taccattact cacaaacagc ttcggcgcaa aaaaagtgtt tgcagaggag
     6601 acagcagctc aagtcatcct tcataaaaag aaaatgactg atttacccga tcctttaatc
     6661 caaaacagcg ggaaagaaat gagcgaattc gatcaatacc aaggattagc cgatatttca
     6721 ttttcagttt ataacgtcac tcaagaattt tatgcgcaac gagataaagg agcgtccgtg
     6781 gatgcagcaa aacaagcagt ccagtctttg actcctggta caccagttgc ttcaggaacg
     6841 acagatgctg atggaaatgt cactttatct ttacctaaaa aacaaaatgg gaaagatgca
     6901 gtctacacga tcaaagaaga accaaaagac ggagtgtcag ctgccgcaaa catggtttta
     6961 gctttccctg tatatgagat gatcaaacaa gcagatggct cttataaata cgggacagaa
     7021 gaactagata ctatccatct ctaccctaaa aatacagtcg gtaatgatgg aacgttgaaa
     7081 gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg gagcagaatt tattatttct
     7141 aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa gtgtcacaga tggattgtac
     7201 acttggacaa ctgatcaaac caaagccaaa catttcatta ctggtcattc ttatgacatc
     7261 ggcaacaatg actttgccga ggcatctatt gaaaaaggcc agttgatcgt taatcattta
     7321 gaagttggaa aatataattt agaagaagta aaagctcctg ataatgcgga aatgattgaa
     7381 aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc aaacaccagt agaaaagacc
     7441 atcaaaaatg atacgtctaa agttgataaa acaacacctc aattgaatgg aaaagatgtc
     7501 gcaatcggtg aaaaaattca atatgagatt tctgtcaata tcccattagg tatcgctgat
     7561 aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta tcgatactca tgacgctgct
     7621 ttaacatttg ataatgattc ttcaggaacg tatgcttatg ccttatatga tggaaataaa
     7681 gaaatcgacc cagtaaatta ttctgtcact gagcaaacag acggattcac ggtttcagtt
     7741 gatccgaatt atattccttc attaactcct ggcggtacat tgaaattcgt ttactatatg
     7801 catttgaacg aaaaagcaga tccaaccaaa ggattttcta accaagcaaa tgtcgataac
     7861 gggcatacaa atgatcaaac accaccgtca gtcgatgtcg ttactggggg caaacgattt
     7921 gttaaagtag atggtgacgt tacatcagac caaacacttg ctggagcaga attcgtcgtt
     7981 cgtgatcaag atagtgacac agcgaaatat ttatcgatcg acccatccac aaaagccgtc
     8041 agctgggtat cggcgaaaga atcagcaacg gtttttacaa ccacaagtaa cggtttaatc
     8101 gatgtgacag gtctaaaata tggcacgtac tatctggaag aaacgaaagc gccagaaaaa
     8161 tatgttccat taacaaaccg tgtagcattt actatcgatg aacaatctta tgtaacagca
     8221 ggacagttga tttctcctga aaaaatacca aataaacaca aaggtacact tccttcaaca
     8281 ggcggtaagg gaatctatgt gtatatcggt gcaggagtag tccttctact gattgctgga
     8341 ctgtactttg ctagacgcaa gcacagtcag atttagtctt tttgcaagaa ttggaaaaag
     8401 gagcagcgga ttacgcaaat gctccttttt cttttattat caaacagtca tcacaaggag
     8461 aacacaaatg aaaccaaaga aaaggaaact tctagatctg ctgatgcttt tcttgcttct
     8521 ctcaggaata gggatactag cttatccatt tgttagcgac gcgctgaata attatctgga
     8581 tcagcagatc atctctcatt atcagcaaca agcggtcaaa gaaaatgaag aagtcatggc
     8641 taaaatccaa gaaaacatga caaagaaaaa ccagcaatta gcaaaagaag gtggaaatcc
     8701 aggtgcagat ccctttacta agaaaaaaga acctgcgaaa aaggatagaa cctattttga
     8761 gaaacatacg atcggtattt tgacgatccc tgagatcaat gtccatttgc caatttttga
     8821 tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta ttggaaggaa cgtcttatcc
     8881 tacgggggga gaaaatacac atgccgttat ttccagccat cgaggactgc ctcaagcaaa
     8941 gttgttcaca gacttaccga aactgacttt aggtgattct ttctatatcg aaatcaatgg
     9001 acggacactc gcttatcaag tcgatcagat caagacggta gaaccgactg atacaaaaga
     9061 tctgagaatc gaaaaagggc aggatttcgt gacactccta acttgtacac cttatatggt
     9121 caacagtcat cgcttacttg tcagagggca ccgggttcct tatcatgcaa aagaagttca
     9181 aaaagaagta cagaaagtat ccgagaacaa acgatttttc ctgtatggat tgttagctgc
     9241 atgtatactg attttcctcc ttcttatata tctttttcgt caatcgttca aaataagaaa
     9301 cagaagttag ctattcctca tttctataat atatctattc gaatcgttcc gtaaacaaac
     9361 aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat gatgatatca aagagagcct
     9421 atcaaacact atctttctta ttactaaatg aggatctttt cttaagtttt ccgatattct
     9481 ttaagaaggc ctttttgcat caattcttgt ttgtttttca agttgcggat attctcttca
     9541 aaattttgag aatatatcac tgagaataac gtatttaata cgtaatcgaa ggcaatttga
     9601 gaggagaaag tcgctacttt agcaaaatca tattcctcct gcgatataac aagacacata
     9661 tcagcctgtt tggccatttc tgaatgctga tttccggtaa tcagcaaggt aggggcctct
     9721 acatgcttca aataggtcat tattcgttca taatgatgga tgcgaccgct gtagctaata
     9781 aaaatggcgc aatcctcgct agtcaaattg gctgcattcc aagaagaatc actgtattct
     9841 tcagccatga tcaaaaactt attcaacttg accattttat tctggaattt ccttgccgtg
     9901 atttgagaat caccttttgc aaaaagaaag attcgctgtg ccttgttcaa tactttaccg
     9961 atttgatcta aatcttcatg ttcaatctgc aattgtgctt tttttattga ctggatcgat
    10021 aaatccgcca ttttctttgc aatttgctga gtgctatctt cacttgaaaa aggaaaatta
    10081 ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa cttcccgccc cagctcgaaa
    10141 cggaattctt taaatccgct gaatcccatt tttttgcata aacgaatgat agctgaatgg
    10201 gaagtatacg tagcttttgc taattcttca atcgtcatgt agactgcagc ttcgatattc
    10261 ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa acaactcttg ttttgttagt
    10321 ttttcttcaa taagcatgat ctgacctcca aattctatct tactgataaa cacttggttc
    10381 cgctactatc tttttgattt ttatccttac tttgaacatt aatttcacca aaccgaatga
    10441 taacaagtgt ttttctgaac ttttttacca gaatagctat tataaatact ttctgcgctt
    10501 ttctaggata tactgaaaac atcaataaaa ggaggcacca ctcatgctta caatcgctta
    10561 tcttggcaat ggaaagagta caaatcgcta tcatttgcct ttttcaagaa aacttacgaa
    10621 caaaatcaat atcaaaaaaa ttttttcccc atctgaacca gtgtggaaaa cattcgaaga
    10681 catcgaatat actgatcagc tgtctgatct ttgggatgat cctgagatcc aattagttgt
    10741 catcactaca cctagtcaat ttcattatga atatggaaaa atggcactcg aacatggtaa
    10801 gaatgtacta gtagagaaac cttttgcaga aacgtcaaag gaagcagaag agttatttgc
    10861 attagcaaaa gaaaaaggtc tatttatcca atgttaccaa aacagacggt tcgattctga
    10921 cttcttgaca gtccaaaaag taatcgaaag cggaaaatta ggggatattt tagaagtaga
    10981 aatgcattat gattatttca gaccggaagt accagaaaat acgcatgaat tttctactgc
    11041 gaacagctat ttatatggac atgcttgtca tacagtagat caagttcttt cttatttcgg
    11101 cgaaccagat gatatcagct atgatgtacg tcaactttta gggaaaggac gaatgaatga
    11161 ttacttcgat ctggattttt tctatggaaa tatgaaaatt tcgataaaat ccagttattt
    11221 ccgcatcaaa gaacgtccaa gtttcgtcgt ttacggaaaa aagggatgtt cgtgaaacaa
    11281 acaaaagatc gacaagaaga acatctgaaa atgttttaca tgcctgatca tgaagacttt
    11341 ggtatcgatt tgcccgaaca ttatggtgta ttgacctata tagatgaaca agggagttat
    11401 catgaagaaa aagtaatttc cgaggttggt gattacagcc gtgtctacga agggatttac
    11461 gagacattag ttaatggcgc agaaaaagtc atcaaagatg aagaaacaat cttacaaatg
    11521 aaaattttgg aagaaggaat caaaagcatt caagaatcag aaggaaaaat atgacatatt
    11581 tgtaagaatc gggtttgttt tttgacgcat aaactgtctt attgtacgat gaaaaagtat
    11641 agaactacta ggaggaacca acatgacaaa ttatgacggt gaaaaacaca aacgattaag
    11701 aggttcaatg gcccctaaaa cagaagaaga agtggcttta gcaggaacta attctggtga
    11761 aacagaagac aaagaagaat cattagctgg cacaaactct ggtgaaacag aaaataaaga
    11821 aacagcttta tctggttcaa attctgtaga agaggaaaca aagcctaatg agcccaatca
    11881 ctttgaaaaa gatatcgaaa aagcggttga agatgtcaaa gaaaaatttg aagaactgac
    11941 ctcaaaaaat aaacatgatc atcattgatc ggacgaaatc aattcataaa agcaggagac
    12001 atttggtcac ctgctttttt tctgtatcca ttcgatagaa atcgttcggt acagtcgtaa
    12061 aaaaaatcca actgtaagtt gtaccaaaca cccctcgatt taaaagcgct accatactag
    12121 aatataagta ggaggtgaac agcgctgtga actacaaaat tagttatctc ctgaaaacac
    12181 caagagaaat cagtctagaa aaagcagcag agatcatcga agtaaataca agcgaggcga
    12241 tcaaacaatt aactcctttt gccgaatatg tggatgatac ccgtatccgt ataaagaaat
    12301 tccgagcaat cgactgggca gaaaaatttt tggatattac tcgatttgaa gctgtgtata
    12361 ccgaacaaga gcgaagagat cttctttatt tgatggttta ttcgactgca acgtctgttt
    12421 ctgtttttca ttttcaagat tttcttcagg tttctaaagg gacagtcctg tcagatatca
    12481 aaaagataag aaatgaatta gaagtggaag attgtcagct gtcgtatact aggaaaactg
    12541 gttttaggct gctagggtca gaagcaatat taagaagaga agcaaaaaat cgcactgctc
    12601 gtttgatgca gtcacttgct ggaagatttg ggctcacgta tttaaccgca accctttcca
    12661 ttgattatta cgccaaagtt agagatttgc tagataaaac gatttcacta tcagatcttc
    12721 gattcgcacc aaatcgaatg gatgaattag cttattattt gagtttgaca accattcgaa
    12781 ttggaaaaac atccatggaa ctggtaaaag atgctgaact gttggcttct cttgcagtca
    12841 ctgacattag tcgacaagta ttgactggcc ttggctattc cgaacatcaa aattcggaga
    12901 ttatattttt ctctctttgc ttagcatcga tcgttgaagg agatgtccag gagcctgcac
    12961 ttgatttttt gctggaatgt tcttcagaag tcatccatcg catggaattc ctgatggccg
    13021 tagaatttgc ttcgtttcgt gaattattga tgaatgtctt ttatcacttg gtccctgcct
    13081 attttcgtat ttcctattct ttttatttac ctaatgtgat gatcgatcaa attaagcatc
    13141 aatatgcttc catctacgaa atgacaagaa aagcattact tccgttagaa agacgaatag
    13201 ggaagagcat ccctgaagaa gagattggtt ttttcactat actatttggc ggagagatca
    13261 gaaaagcgga cgcagaagaa agaaatagaa aaataagagc cgtgatcgtc tgtccaagtg
    13321 gcattagctc ttcattgatc ttaaaaagtg aacttcagca attattcccg atgatctttt
    13381 ttacagagac taattcagct gaccgcttac acgaggttga cgaaacttct tacgatatta
    13441 ttttttctac tgtacctctc acacttactg ataagaaaaa gtctctctat gttctgcatc
    13501 caatcatgac tgacttggaa aaaaatcagc tgatcaatca agtacaaaga gattggctga
    13561 taccaggatt ttcaatgcct tctgcccaag aattgttgtc ggcgctactg ccttatatcg
    13621 aattaaaaga aggcgtagac gaagaaaagc tctatcaagt attaaatcgt aaaatgaaca
    13681 aacttttaga aaagaaggag gatcaacgtc ctatgctatc tgaattatta acagaagaaa
    13741 tgatccaatt atcggatcaa ccaaaaaact ggcaagaagc aatcacattt gccgcacagc
    13801 cgctattatc agaaaataaa atagaagaaa gttatatcaa agctatgatc gagcgcgtgg
    13861 agcagtatgg tgcgtttatc catattggcg atcacatcgc attacctcat gctcgtccag
    13921 aagatggcgt aaacgaagta gggatgtctt tgctgaaact tcaacaacct gtggatctgc
    13981 ttgataatcc agcccatccc attagtttgt tcatctgttt agctgctatc gataatgagg
    14041 cacatcttcg ggctttggca aatttaacaa agctactttc taataaacaa aatcttcaag
    14101 atttattaca cgcatcaaca aaagaagaaa tattagcgat cataagaaaa ggagacgaat
    14161 gatatgaaaa tttttgcagt atgtcaaagt ggattaggaa caagttttat ggtagagatg
    14221 aacatcaaac aagcacttgc agatcttgga gcagatgtgg acgagtttga agtaagtcat
    14281 acggatgttg gaagtgtatc tagcgatatg gctgattatt tcttcattga gcattctttg
    14341 aaagatacgc taacttctat acctgatgaa aagctcgtac ctttgcaatc gatcattgac
    14401 agcgaagaag taaaagaaaa agtaacgaag atcttgtcaa aagaataaaa atgattcaag
    14461 ggaggaacta tcaatggata aattgttgaa tttgtttgtg tcaatcgtca gtcagccctc
    14521 catcctcgtt tctttgattg cactgcttgg tctcgttctt cagaagaaga aattttcaga
    14581 agtgatccaa ggaactatca aaacatttgt cggattccta gtactgatcg gcggtgctgg
    14641 tttaatcagt aattcactaa ccccttttgc ttcgatgttc caaacagctc tgcatactca
    14701 aggagtagtt cctagcaatg aagcagtagt ggcgattgct ttgcaacaat acggtacacc
    14761 tactgctttg atcatgctag tagggatgat cgtgaatatt ttacttgcta gattcacaag
    14821 atataaattc atttttctta caggtcaagc aatgctttat gtttcttgtc taacagcagt
    14881 cattttagta agcgcggggt ttactgcaaa tttgtggatg atcttattag gcggggtctt
    14941 cgaaggaaca ctattaacta ttacgcctgc tctttgtcaa ccgtttatga agaacatcac
    15001 tggaaacaat caagttgcgt tagggcatac cggaaatatc ggttatgctt ctagtggatg
    15061 gatcggaaag ttttttggaa acaaagaccg ttctacagag gacataaaga ttcctaaaag
    15121 tttcagcttt ttgcgagatt ctactgtatc gatcatgctc ttgatgtcga tcgtatatgt
    15181 cgcacttgct ttgttagctg gtcctggatt tgttgaaaga gaactaagca atggagacaa
    15241 cgctgtaatc tttgcactga tccaagctgg aacattcaca gctgggtttg ttatcgtcct
    15301 tcaaggggtt cgaatggtgt tggcagaaat cgttccagca ttccaaggga ttgctaaaaa
    15361 actcgtaccc gattctaaac cagcattaga tgttcctatc gtttttccat atgcacctaa
    15421 tgctgtatta attggctttt ttgttagctt ctttgtagga acactatcca tgtttgctat
    15481 gataggcttg caaactacag ttatcattcc tggggtagta ggacatttct tctgcggagc
    15541 tgcttctggt gtcttcggca actcgactgg cggtagaaga ggggcggtat taggttctgc
    15601 tttcaatagc ttattgatta gttggctgcc attatttatc cttccagtat taggagatct
    15661 taaaatcgct tcttctacat ttgccgatac tgattatcta gtaccaggac ttgtacttgg
    15721 gaaactagga gaatacggtc aaatagcctt gacagttgga atcatcgctt tctttttact
    15781 tgtcgtatta tctagctttc tttttgccgg aaaaagaaca aacattgttt ctaaagaaca
    15841 gaattaagca aacttcaaaa taaaaattgc ttggacaata tcagaagaag atttaaagtc
    15901 ttctgatatg tcgcaagcag ttttttgttt tatgcattta tttttgaata gcttgttaag
    15961 acaagtggct gttttgctag aagaggataa cttttaccat tcattttcac tgttccgcct
    16021 gttctatcag aagtaatcac gatcttatct tttgtttttt caagcgaaat ctttccatct
    16081 ttgataggta gcgtacaatt cagtgtttca aatgaatcta gattaggttg tatcataaat
    16141 tcttcataac ctggctttgt tggatacaaa ccgataaaat agcgtcctaa aagatagatt
    16201 gggcttgctc cccatgcatg gcataagctt tttccataag gatctccata catactgtac
    16261 ttttcatttc ctgtttgagt agggtcgtat tcttcccaga aagttaccgc atcatgagat
    16321 aacatccctc cccaataatc aagaatcgtt tggtagacga tattcgtctg tccgagtttg
    16381 cataaagcat cttgttcata gaatttgaaa tatggagtag taatctgtgt gatagtgtca
    16441 ttcaataaga cattctttaa gatcgattgc tgttggtctt tgttagcaaa atcgaataaa
    16501 acagcgaaaa tattcgcatg acgcgtcaca tttcttctcc cagattcata gcaatcgata
    16561 aaagcgccaa gttcttcatc ccaaaagtag tcaaataggt tttgtttcaa tagttgatat
    16621 ttttcttgat agagatgagt atgataattt tcgttctctc catatgtttc agctaagata
    16681 tctccgcaag tgatcatgga atgataggct ttcagtaata agacttgttc tgcagcaagc
    16741 gttccttctt tatccatttc agaccaatca ataaatatcc aatcttttgc tctcccataa
    16801 ataaacccta attcattcgt ttgctccatc aagtatctca ttaatgattg catcttaggg
    16861 tagacgatgc gtaaaaattc atagtctttc gtcatcatat agtggttttc gataccaatc
    16921 aaccacaaga tcgaatagtc aacgatagta ttcatatgct gcttgatttc attttgtcct
    16981 cttaatgcta atatcgttcg tttattgatt tcctcgtcaa aaaaaagata ttgattgatc
    17041 atgaatgatt gatacgcatc tccagaccaa atccagcgat ctcgtttgat accgtcgata
    17101 aaaaacaaat cactacataa cttgaaggct tcttctgata ctgaccagat ctgattcagt
    17161 aaagaagaat cgcttgaaaa agcagcttgt ttcgaaagag ataaaaattc atgaaaaact
    17221 tgaagttgaa ccattccagt agaaacctct ggtataaaca aatagcgaaa agcgcgttta
    17281 cgagctttca tttttcttgt cattcctatt tgcttatagt agcaataatc gatatccata
    17341 gcttctacat cggactctcc ataacataga gtgatagatt cgatatggtc atccagagat
    17401 attagttcta cacagccatt cactgctcgc ccaaaatcga ataagattcc tccgccaatc
    17461 tccgtttcag tgactggatg gacgcattct ttttgataat agatttgatt tggatctttt
    17521 gcgatatcga cataaagcgg actccagcca gctggatatt cttcaataaa attacttgct
    17581 gtccagccaa tatctgattt tacttcgtct ccctcgataa acatcgctgg tagccctgat
    17641 gtatgtccta caaagatacg aatttttgct ttaccagctg gacaagtcag ccattttcca
    17701 catggatgtt tttgtccatt aatttctaca tacccaactc cttggatcgt cactttaaac
    17761 atggaaggtt gatcaagaaa gtaatatctc ttaaacttga cgttccgatg gcaatcatcc
    17821 atgtaccagt acgctggcca gtcaaaacct cgttcttccc gttggaaatt ttgtagcagt
    17881 ccatggcgga tctcaaagtc tccaggatac caaagccatc tatctttatg tttttcattt
    17941 gtcattttac acgacctcca tcaaatttat tttgttgtca attgttcttt tatttcttca
    18001 tctactctta tcactgtctt tctattcgtc ctgtgtacta ctagaaggct aataagcgcg
    18061 ctaatcaaca gcaagatact gccattgacg aaaacagcaa gtgttttact cccaagagaa
    18121 gcaaaagaga gaaagagcaa accatatggt gcaatgaaag tacctaaatt agcgccaacc
    18181 aataagattg aagtattgaa actctccgcg tacttgggcg cttggatact tacttggttg
    18241 aataagtaag gaatgaataa cgaaaaagaa attcctgtta ggatcgcacc aaacccaatc
    18301 acatacaggt ttatcacagt ggcaatcaat acaaagccga ttcccatcag tagtaaagaa
    18361 agtgtaagca tatagcgatg tgcttttgtc tgaagccagc caaaacaaac tccggcagcc
    18421 atacttgcca attgcatgat tgttaagata ttcgctgcat tagtttcgtt tcctagagaa
    18481 taagtcgtca ggactgtggc taatttaatc gatacagcat tatataccac gatgaccagc
    18541 aagagaaaaa taccataact aatcatttta ggattcgttt tgattacttg tcttgttggg
    18601 gctgtgccat gcgattgaag ttcagttgtt tcaggtgtag gaacaaacaa aacaaacaag
    18661 acaagaatag gtaagctgat ggcgtagact aaaaaggtat attgccaacc gaactgtact
    18721 agctgtcctg caacaaatgt cattaaagct gcaccaaatc cttgaaatgc actttgaaaa
    18781 ccaatcatag aggctcgttc atctccgtga taaaaggcag caatcatgct gactgccaaa
    18841 gaattgatca atccaaagcc aatacctagt ccagctcttg aagctaagac aatactgtaa
    18901 ctttgtgaga agagggggag gataccacaa atcgatgcaa tcactaatcc gatgatcaca
    18961 gtttgttttt gtccgatttt cctagcaata aaactactaa tcaaaacaaa aatagtgacc
    19021 ataaaagagg gagcagtcgt taataattca acagcagata aagaacgcgt ggcataagta
    19081 gctgacatac tgggaattgc agacgtgact gcaccagcag aagtcaaaat aagagagact
    19141 gacagcagag aaaatttcat catcttttta ttcataagca tgttcctttc atttttgtaa
    19201 gcgcttatca aaaatatagt atgaagtggt tattctctca atagcggaat cacaggaagt
    19261 tcatgtccca accatagaaa gtctttatga aaccaaaaat agctttgcat ataatagcag
    19321 tacagcgtga actaataagc gaaagaaacc gcttacaaaa aggagggagt caattggaac
    19381 tagctgaatt agacattttt ttgcgtaacg aagaatcaat cgaaactctg cagcgtgaac
    19441 aaggaatgaa tatcaatgat ttaaacttag cttaccatct tcagacaccg ccaaaaatcc
    19501 accgagggga tctatttcaa cagggaaata ttcatatcag taaacaccgt cgatatgcgg
    19561 aagttcccga gcatacacat gattttgttg aaatcaatta tatgtatagc ggtacatgtt
    19621 tacaaattat taatggagaa aggatagaga tggaagaaag tgacgtggtg atcattgata
    19681 aagaagctac tcaaagaatc gaatacacag ggcaagagga tattttaatc aacatcttat
    19741 taaaagaaga aaccttatct acagagatat tgaatcattt ggcaagcaca aataatctac
    19801 tttcttcttt tttgataaat gcatcaaaag aaaaagggga acatacacaa ttcattcatt
    19861 ttcgcggcgg aaaaacaagt cgtttgacgc atttgattga aaatatgctg ttgatttatt
    19921 ttgatacata cagtcataag atccgttcgt taaatctcta tacatcacta ttactgatcg
    19981 aattgactca tttattagaa caacaaagct tttcatcagc agatggatgc gcagaaaatg
    20041 agatgcttag agtattagat tatatcgata agcattttac ttcttgtacg ttaaaagaaa
    20101 cagcagctca ttttggctac aatccggtct atcttagtaa taaattgaaa atgtattttg
    20161 atgcttcctt tcaagaactg gttctaaaga aaaaattcga ctttgcgttg gaactgattc
    20221 aagaaacgag tttttcgttg gaagaaatcg ctaatcgtat cggatataaa caaacgactt
    20281 ctttattcaa attattcaag aagtattcaa catgtactcc caatgaatgg agaaataaac
    20341 attaagaaga aaatctttca ctaaagcata tgaaggaaaa ttgttttagg tgcgattgct
    20401 gtcttattat atcgttgcag cgaatttttt tggatttatt ttcagttcta aacttttggt
    20461 tgtattatca ggggatatag gtgtaaacat ttgtcattta tctgttatgc gtattcagga
    20521 attcgtttcc atcaaaaatt ataataacaa ctattataat taacatgttg ataaaatgaa
    20581 taaacaagac tatacttttc atgaaagtaa caaatagtta tgaaaaggag ctgtttattc
    20641 atgaaaaaaa caagtcgtaa agtagtaatc gtcggtacag gttttgttgg aacaagtatc
    20701 gcttatgcaa tgatcaacca aggaatctct aatgaattag tattgatcga tgtgaatcaa
    20761 gaaaaagcag aaggggaagc attggacctt ttagatggta tggcttgggg cgatgaaaat
    20821 gtggctgtat ggtcaggtgg ctatgaagaa tgtaaagatg ctgatatcgt tgttatcaca
    20881 gctggtatca accaaaaacc tggtcaatct cgtttagatt tagttaaaac aaatgcgtca
    20941 atcatgcgcc aaatcgtaaa agaaatcatg ggatcaggat tcgacggtat tatcgttgtt
    21001 gcttcaaacc cagtagatat tttgacatat atcgcttgga acgaatctgg tctgccaaca
    21061 tcacgtgtta tcggtactgg aacaacattg gatacaactc gtttccgtaa agaaatcgca
    21121 ttgaaattaa aagttgaccc acgcagcgtc cacggctata tcttaggtga acatggtgat
    21181 tcagaagttg ctgcatggtc acatacaact gtcggtggta aaccagtgtt tgaaattgta
    21241 gaaaaagacc atcgtattgc ccaagatgaa ttagatgtga ttgctgataa agtccgtaat
    21301 gctgcttatg agatcattga tcgtaaaaaa gcaacttact atggtattgg tatgagtact
    21361 gcacgtatcg taaaagctat cttgaataac gaacaagctg tattgcctgt atctgcttat
    21421 ttaactggtg aatacgacga aaaagatatc tttacaggtg tgccatcaat cgttgacgaa
    21481 aatggtgttc gtgaagttgt tgaattatca atcaacgaag aagaaaaagc aatgttcagc
    21541 aaatcaacaa gtgctttaag agaagtattg aacactgtat tataagaaac aatgaatatg
    21601 aactaaaaat cctaaaactg cttaacagct gaaatagctg ttaagcagtt ttttattgat
    21661 atctaatagg taagcaaata aatagaatgg ttaaaattaa tcggtccgaa aatgtatccg
    21721 tacatatgaa catatgaaat ttaatctgtt gattacaata agcgaatcgt tatcaatgtt
    21781 tatctgtggt taattaaaaa atccttccgc aacggcaaat acaatgattc atatctaatt
    21841 cgttcatatt tgattaactg aataaagaaa ctggttttta tcattcaatt aaacgacaaa
    21901 aaaagtaaga aaaactgcaa aattttttta taattttgca taagcagatt ttgatggaaa
    21961 aatgacgatt tttgataaaa aaccgttcga gag
//
LOCUS       DAZKMB010000040        21833 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000040 DAZKMB010000000
VERSION     DAZKMB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.040"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACG8V3_001321"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACG8V3_001321"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0299868.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACG8V3_001322"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACG8V3_001322"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0299869.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACG8V3_001323"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACG8V3_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0299870.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACG8V3_001324"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACG8V3_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0299871.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACG8V3_001325"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACG8V3_001325"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0299872.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACG8V3_001326"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACG8V3_001326"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0299873.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ACG8V3_001327"
     CDS             complement(8054..8524)
                     /locus_tag="ACG8V3_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0299874.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ACG8V3_001328"
     CDS             complement(8570..8995)
                     /locus_tag="ACG8V3_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0299875.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ACG8V3_001329"
     CDS             complement(9062..9331)
                     /locus_tag="ACG8V3_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0299876.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ACG8V3_001330"
     CDS             complement(9342..10937)
                     /locus_tag="ACG8V3_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0299877.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACG8V3_001331"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACG8V3_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0299878.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACG8V3_001332"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACG8V3_001332"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0299879.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="ACG8V3_001333"
     CDS             15403..16377
                     /locus_tag="ACG8V3_001333"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0299880.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="ACG8V3_001334"
     CDS             16547..17947
                     /locus_tag="ACG8V3_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0299881.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="ACG8V3_001335"
     CDS             complement(18025..18747)
                     /locus_tag="ACG8V3_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0299882.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="ACG8V3_001336"
     CDS             complement(18826..19332)
                     /locus_tag="ACG8V3_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0299883.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="ACG8V3_001337"
     CDS             19455..21254
                     /locus_tag="ACG8V3_001337"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0299884.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..>21833)
                     /locus_tag="ACG8V3_001338"
     CDS             complement(21322..>21833)
                     /locus_tag="ACG8V3_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0299885.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6049 a         4791 c         3964 g         7029 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agctttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt cccctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggaa
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttacac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggttgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttg
//
LOCUS       DAZKMB010000041        21784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000041 DAZKMB010000000
VERSION     DAZKMB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.041"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(96..506)
                     /locus_tag="ACG8V3_001339"
     CDS             complement(96..506)
                     /locus_tag="ACG8V3_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0299886.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            781..993
                     /locus_tag="ACG8V3_001340"
     CDS             781..993
                     /locus_tag="ACG8V3_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFQ0299887.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            996..1208
                     /locus_tag="ACG8V3_001341"
     CDS             996..1208
                     /locus_tag="ACG8V3_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299888.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1348..1503
                     /locus_tag="ACG8V3_001342"
     CDS             1348..1503
                     /locus_tag="ACG8V3_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299889.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(1500..1724)
                     /locus_tag="ACG8V3_001343"
     CDS             complement(1500..1724)
                     /locus_tag="ACG8V3_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0299890.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            1802..1990
                     /locus_tag="ACG8V3_001344"
     CDS             1802..1990
                     /locus_tag="ACG8V3_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299891.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            1987..2160
                     /locus_tag="ACG8V3_001345"
     CDS             1987..2160
                     /locus_tag="ACG8V3_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299892.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            2129..2257
                     /locus_tag="ACG8V3_001346"
     CDS             2129..2257
                     /locus_tag="ACG8V3_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299893.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2316..2651
                     /locus_tag="ACG8V3_001347"
     CDS             2316..2651
                     /locus_tag="ACG8V3_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299894.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            2884..3825
                     /locus_tag="ACG8V3_001348"
     CDS             2884..3825
                     /locus_tag="ACG8V3_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFQ0299895.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            3827..4717
                     /locus_tag="ACG8V3_001349"
     CDS             3827..4717
                     /locus_tag="ACG8V3_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0299896.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            4760..5560
                     /locus_tag="ACG8V3_001350"
     CDS             4760..5560
                     /locus_tag="ACG8V3_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0299897.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5575..6426
                     /locus_tag="ACG8V3_001351"
     CDS             5575..6426
                     /locus_tag="ACG8V3_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0299898.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6492..6626
                     /locus_tag="ACG8V3_001352"
     CDS             6492..6626
                     /locus_tag="ACG8V3_001352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299899.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            6627..7088
                     /locus_tag="ACG8V3_001353"
     CDS             6627..7088
                     /locus_tag="ACG8V3_001353"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0299900.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7114..7566
                     /locus_tag="ACG8V3_001354"
     CDS             7114..7566
                     /locus_tag="ACG8V3_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299901.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            7672..7866
                     /locus_tag="ACG8V3_001355"
     CDS             7672..7866
                     /locus_tag="ACG8V3_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299902.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            7897..8190
                     /locus_tag="ACG8V3_001356"
     CDS             7897..8190
                     /locus_tag="ACG8V3_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299903.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8121..8660
                     /locus_tag="ACG8V3_001357"
     CDS             8121..8660
                     /locus_tag="ACG8V3_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0299904.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            8735..9202
                     /locus_tag="ACG8V3_001358"
     CDS             8735..9202
                     /locus_tag="ACG8V3_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0299905.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            9949..10179
                     /locus_tag="ACG8V3_001359"
     CDS             9949..10179
                     /locus_tag="ACG8V3_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299906.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10338..11171
                     /gene="terS"
                     /locus_tag="ACG8V3_001360"
     CDS             10338..11171
                     /gene="terS"
                     /locus_tag="ACG8V3_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0299907.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11164..12579
                     /locus_tag="ACG8V3_001361"
     CDS             11164..12579
                     /locus_tag="ACG8V3_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0299908.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            12591..14120
                     /locus_tag="ACG8V3_001362"
     CDS             12591..14120
                     /locus_tag="ACG8V3_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0299909.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14038..15090
                     /locus_tag="ACG8V3_001363"
     CDS             14038..15090
                     /locus_tag="ACG8V3_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0299910.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15092..15409
                     /locus_tag="ACG8V3_001364"
     CDS             15092..15409
                     /locus_tag="ACG8V3_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299911.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            15536..16159
                     /locus_tag="ACG8V3_001365"
     CDS             15536..16159
                     /locus_tag="ACG8V3_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0299912.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16174..17064
                     /locus_tag="ACG8V3_001366"
     CDS             16174..17064
                     /locus_tag="ACG8V3_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFQ0299913.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17087..17302
                     /locus_tag="ACG8V3_001367"
     CDS             17087..17302
                     /locus_tag="ACG8V3_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299914.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17314..17655
                     /locus_tag="ACG8V3_001368"
     CDS             17314..17655
                     /locus_tag="ACG8V3_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299915.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            17655..18026
                     /locus_tag="ACG8V3_001369"
     CDS             17655..18026
                     /locus_tag="ACG8V3_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299916.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18019..18414
                     /locus_tag="ACG8V3_001370"
     CDS             18019..18414
                     /locus_tag="ACG8V3_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFQ0299917.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18416..18793
                     /locus_tag="ACG8V3_001371"
     CDS             18416..18793
                     /locus_tag="ACG8V3_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFQ0299918.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            18794..19402
                     /locus_tag="ACG8V3_001372"
     CDS             18794..19402
                     /locus_tag="ACG8V3_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0299919.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19402..19743
                     /locus_tag="ACG8V3_001373"
     CDS             19402..19743
                     /locus_tag="ACG8V3_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299920.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            19985..>21784
                     /locus_tag="ACG8V3_001374"
     CDS             19985..>21784
                     /locus_tag="ACG8V3_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0299921.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAI"
BASE COUNT         7840 a         3207 c         4519 g         6218 t
ORIGIN      
        1 ttatatggat taatcgcatc agatccaaac tctacaaata atttttctat ttcttctaat
       61 atccagtatt tgtttaaatg cataccaaag cccacctatt gacgatattt tttaggagtg
      121 aattttttct ttgctttaac tttggttaag gcaagtgcaa gttgaatggc atcgtataat
      181 ttttccttat cttcatcgtc taaaggttca ccataataat ttgtttcagc atctgaattc
      241 aagccttcaa gtaatttttc agcttgtaaa gcaatatctt gtttatcttt ttctgttaaa
      301 tcataataat ggcgtttatc agtacgtcct aaaagataat cagttgatac attaaagtaa
      361 tctgcaactt tttgtatttt atctccagat ggagtacttt tattccatct agacatgctt
      421 ccatttccga aatttaactt tctttcaagt tctgccaacg tcattgaacg ttcttttaat
      481 aagattttta tacggtctaa tagcatagga tatgcattcc tttcaaaata ccgacaaaaa
      541 aagtattttt ctgcaaatta tctatttaca aatagagaat ttgcagttat actagttttg
      601 taagcaagat atttacggca tacaaagact aaaagcaata aactaatagt ctccccagac
      661 atcagtaagc tcgctttttg tgcgctgtat tttgtatgct tacataatag catatttgca
      721 gtttttgtca aacttattag acaaaaatat tagaaaatat tcgataaggg ggtatgaagc
      781 atgactttaa aacagcaagt attaattgct ttagtaaaga aaggttggag tcaaagagag
      841 ttagctcgac gaatgggtat atcaattact tatttacgag atatcttcat cgaaaaaaga
      901 aaacctaagg aacgtttaaa acaaatcgaa gaacttttgg atatcaagtt agaagtagat
      961 tctaatgatc agccatcaga acaggaggcg taaaaatggt acaacgtttg agcaaagcgg
     1021 agtttaaaga gaaatataag tattctgaaa gtacttatca aagaagaatg aaggaattta
     1081 aaaaatcacg ttttagtgag gggtataacg cagttacttc caaagaaata acaattgatg
     1141 tcgatttata tgaacagttt agaacatggc gttctaaaaa tcgatttaga gttagagctg
     1201 tttgttaata gctaaaaaat ctaaattcac cacttctgaa aagaggagtt gctgctctcc
     1261 tttcaagttt gccaaagtcc acgttttttc attttggcaa ctcctcttct tggaggtggt
     1321 gacggcgaga gacgaaggag ggatgacatg aaggaagaat caaaaatagg gattttgaat
     1381 gagaacaatt acacaagatt aattattgaa acagaagaag ggaaaaaagt agcagaagtt
     1441 acattgaccg atgcaactcc tgctgacggt tatcgtattc gattaactcc taaatatgat
     1501 taaccttttg gtgggtgtgg atcgtttccg tgactatcac ggctttgaat ttttccatct
     1561 ctgccatgaa taaatagctc agatccttga tttttagaaa tctctctagc gatttttact
     1621 gcttctgatt ttgtattagt atgtgcagtc gctttagaat ttccagcacc tttgacattc
     1681 caaccaccat tggaattagg tacaacatgt tgattttttc ccatagtttt cacctccatt
     1741 tctatagaaa tattttatca aatagaaatg gagaaattca ataaacgaaa ggagaagtga
     1801 tatggcatat accttgcaac aagaacatca aattctcggt ttgattaaac aacgcaggaa
     1861 acaattacaa gatgaccgtg cagcgcttag aaaagccgat gagctatcag ataggcaagc
     1921 tgaactaatt gcttctgaac ttgaggattt gagaatgcta gaaataaaaa atagggagat
     1981 tagattatga agaagacaga cacacttttt ataggattca ttttggggtt attagtgatt
     2041 gtagcgcacc aaagtattat cgggggaagc ttgttcgcag cattgatggt tttaatcaat
     2101 ctgcttgatt caaaagaaag gagcaactat ggcacgagaa gaagcgctaa aaatcggtaa
     2161 agtgattgct gataatcggt ggggaaatag ccgccctatt attttaagca agcaacatat
     2221 cgaaaagcaa aaagcatggc aacaaataaa aaagtgactc tgccagccag caaagagtca
     2281 catacaaatt tataaaacta ggagaattat atcacatgaa taagaaaatt gaaaatctta
     2341 tcgaagaatt gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt acagcccaaa
     2401 aagaagggga acttaaaagt ctcgtttatg gtgagacaac agaaatttta ctttgtcttg
     2461 ctatgcaaga agaacattta gatgaaaatc tcccattgtc agcacatatt atgcgtagaa
     2521 ttgctgtgga tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct tcaaatcata
     2581 cttttgttat taataacaag gaagatttag ctgatgtgat gactcgtatt ctcaaggggg
     2641 aatttcaatg atgaatgaaa aaacaattga tctactcatt attttatttt tcacgttagt
     2701 gattgtagcg aaaagttatg taatctttgg cttgttattc gcaattacca tgcttgtgct
     2761 ttgcgtgtta gcaagcagaa aagaggttaa acatgaacga gttagcaaat cttgacaact
     2821 atttaactga tcctgattat gctaaaccac catatgaagc accaattgat gaggaggatg
     2881 aagatgagta aatctacctt agaaatgagc catcaagaat ggcttgaaga ccgtaagaaa
     2941 ggcatcggag gttcggatgt tggaacaatt ttaggattga ataaatggaa atctccttat
     3001 caactatggc tagaaaaaac aggacaagta gtacttgaag aatcaggaag tgagcccgct
     3061 tattggggta atgttttaga agaagtggtt gctaaagagt ttcaggaacg tacaggcaaa
     3121 aaagtacgca gaagaaacca agtatttgaa catccattac atccattttt aagagcaaat
     3181 attgatcggg aagtagtggg agaaaatgcc attctggaat gcaaaacagc caatcaattt
     3241 ctcggtaaag aatgggaagg cgaagaggta ccgcttagct atctctgtca agttcaacat
     3301 tacatgaacg ttctaaacaa agactattgt tacatcgctg tcttaatcgg tggtcaaaaa
     3361 tttatctgga agcggattga acgagatcaa gagctgatcg atacaatcac tgaacaatta
     3421 gtagagtttt gggaaacaaa cgttcttgga ggtatcgagc caattattga tggtagtcaa
     3481 gcgacagctg atttcttgaa agagaagtat gcagatgttg aagacgttca aacgacgtta
     3541 ccaattcatt tcgatgaact agttgaacag aaaaacgaac tcaaacggac taagaaagaa
     3601 attgaatcag ctatccgtca agtagacaac gagattatca gcgagctagg taaacgtgaa
     3661 gccagtatcg gtatcactca aaaaaacatc atcagttgga aatttgtccg tacgagacgt
     3721 atgaactcga agaaactagc agagaaatat ccagatgtcg caaatgatga agagatttat
     3781 aacgttactg aatcaagaag gctaaccgaa agggagatca aataatatgg caacaaatga
     3841 atcgttaaaa aatcaattgg cagcaaagcc acagaaacaa gttgcaccag gtcaattagg
     3901 acttaaagct ttgatgaata caccaacaat gagaaagaag ttcgaagagg tgcttcatga
     3961 caatgctaat gcttttatgt cgaatgttat gactcttgta tctaatgaca gttatcttgc
     4021 agatagtgaa ccgatgtcta tcatgagtgg tgcgttaact gctgcaacat taaatcttgg
     4081 gctagataag aatttaggtt atgcatattt agttccattc aatagtaaaa acaagcaaac
     4141 aggaaaatgg gaaaagaaag ctcaattcat gcttggctat aaaggatata tccaattagc
     4201 ccaacgatca ggtaaataca aagcattaaa tgtgatcgaa gtttacgaag gagaactaaa
     4261 aagctggaac cgactgacag aagagtttga gtttgatcca aatggtagaa cgtctgatga
     4321 agtcattgga tatgttggct atttcgagtt actgaatgga ttcaaaaaaa ctgtctattg
     4381 gaccaaacaa gaaatcgaag ctcatcggat tgctaacaat aaagatcgcg ataagacaaa
     4441 gttaagtggt gtgtgggcat ctgattacaa tgcaatggca cgaaaaactg ttttgagaaa
     4501 tcttctttct aaatggggaa tcttgtccat cgaaatgcaa gaagccacca catcggatga
     4561 gagagtccaa agggttcaag aagacggcag cattattgct gaaacagaag ttgaagaaga
     4621 tattcctgaa agaaaagaag cagaggttat atctgaagaa aatgaagatg tacaaactgg
     4681 attatttgat gcatctaatc cgccgttaaa caaataatga gggagctttc tcccttgctt
     4741 ttctagagga gaaatacgaa tgaacacagg atatgtcaaa ttacatcgaa aagtgatgaa
     4801 ttcattcgtt tggaccaatc cttatatgta taaattatgg aacttgtgtt tgatgaaagc
     4861 gagtcatgag aatcgcaaga tactttttaa tggaaaagag atacaagtga acagcggaga
     4921 attcgtcaca gggcgcgatg ctatcacatc tgagatgaac gaaggcgtga aacgtgaaca
     4981 tcaagtgaac agcggttctg tatggagatg gctaaaacaa tttgaaaaaa acggaatgtt
     5041 gaacatcaaa tcaactacga aatacagcgt tatatcaata aaaaactggt ctttgtacca
     5101 aagcagtgaa caacaaatga acatcaaacg cacaacaagt gaacaacaag tgcacacaaa
     5161 caagaatgaa aagaattata agaatgaaaa gaataataaa acatcatcgt cgcaaccacg
     5221 caaaaagcgt gtttacgaca ccgactcagt ttactacatt ctcgcggagg agttattcaa
     5281 acagatttgt cagaatcagg aaatcaaaaa gccgaatctg caaagttggg cagatgacat
     5341 tcggaaaatg atcgagatcg acaagcggac cgaaaaccag gtacggggaa tgattgaatg
     5401 gagtcagcac aatgtgtttt gggcatcgaa tattttatct gctaaaaaat tgcgagaaaa
     5461 atacgacaca atggcagcgc aagcaaatcg tgattataaa acaaaacaaa ctaaaacgct
     5521 tgaatacgag aaatttagca cagatgagtt gcctatttga gaggaggcgt aggcatggaa
     5581 actgttgggg aaatcatgga aaagctgata cagaaagtgc ttgtccaacg tggcgaatgt
     5641 cctgaatgcg gacagccttt gtatggatgg cgtacgaaga accctgatgg ctcagaacgt
     5701 tgtaagccaa cctgtatgca atgtgggtat aaagcattgc gagttcagga agacttacaa
     5761 acagaacgaa tttacaacga gagcctgaaa gcaagagcaa tcaatttttt caaaggtggt
     5821 tctgttgtgc ctaatcaagc gttgtttgat tgcacattgc agaattatca aattgtcgat
     5881 caagaaacaa gacaagcggt tgaagtaacc aaacgctttg ttaattcagt cttgttagga
     5941 aatccaagtc accttgtttt aactggaaaa caaggaacag gtaaaagtca cctagcgatg
     6001 gcagcggctt gggaagtctt gaagcgatca aactacgata aaaaaatctt atttatcggg
     6061 ttacaggaaa tgctggatca aatcaaattt tcgtacaaca atcctgaact cagaaaaacg
     6121 attgagggat cgttgattgc agatatcaaa acagcggatt tagtcatcat tgatgatatc
     6181 ggttcagaac taggaaaaga tgcatcagat agtcgagcgt ttggcataaa cacgctaaat
     6241 tcgttcttgg atgcacgaca gaacctagca acgattatca caacgaactt gcttggtgaa
     6301 gaactgaaaa aagcttacgg tacgagaaca atatcaagaa tgtttgtcaa ctctgatgga
     6361 tttacgatgg tattttctca aacagcagac aagcgcataa aaccagtcaa aggagcgatc
     6421 gcatgaattt gactaaacaa gaaaaaagaa tctgtcgtca gataatgttg caaattatag
     6481 gtgatcgtga catggacgaa ttatctcaac aagagagtcg aatggttcgg aagataagta
     6541 agctaattat ttatcgggat acagaaagat ttctcactgt tttaggtaac gtctacgaga
     6601 atccagagtt attggaggta gagtaattgc caaaaatatt agatgcatgt tgcggtagta
     6661 gattgttctg gtttgataag aacaattctg acgtaacatt tatggattgt cgtcagcagt
     6721 atgaagaatt atcaacaggt catgtgatca atgttgatcc agatgtggtc gcagactttc
     6781 gcgatatgcc ttttgaagat gatgtgttcg atatggttgt atttgacccg ccacacttaa
     6841 gacatgtcgg cgaaaactca tggcttgcca aaaaatatgg aaaattagac gaactttggc
     6901 cagaagacat caggcaaggt tttgcagagt gtatgcgagt tttacgacct tcaggaacat
     6961 taatttttaa gtggaacgag gaacagatac cactttctga tgtgttagag gctattggag
     7021 aacagccatt gtttggtaat aaacgaagca aaacacattg gcttgtgttt atgaaaaaat
     7081 tcagctaacg gaggaaacaa aggaggaaaa gtaatgaaga ttgtattaaa taaatgtttt
     7141 ggtggctttg ggttgagcca tgaagcaaaa atggaaattt ttaaaagaaa aaatattgaa
     7201 gtattcccat acatcaataa ctttagctat gactcagatg atgaatacac gagatacaca
     7261 ggccaaaagc taggttcgat ggattttatc tactatttca aaaaagaccc gaaaatcgac
     7321 aaagttacag gaatttatag cgaaattgac cgactgtacg gaattgctga tgattccagt
     7381 ttttcaagtg attcaaacag aggtgataag gacctcgttg ctgttgtcga aaaactagga
     7441 agtgaggcca gtggaccata tgcaagtctt aaagtcgttg atatacccga tggggctgag
     7501 tgggaaataa gtgattatga cggcgttgaa actgcacatt atggttttca aacaggtagt
     7561 atttagtcca ctaatgacaa ctaaagagga ggcagaagga tgagtgaaga tgaatacttt
     7621 gaacagtagt ctattaacga cagagatttt taactggaaa ggggtgtagt catggacgca
     7681 tcggaagtag taaataaaat taatagtctc aaagaggttt tcggagacgt cgaagtagtg
     7741 ataaacgcac aaggctatga caacgaattt tttaagcaga ttaccgacat tagcattcag
     7801 caaggaatgg aagacgaaga cggaaacttt atcgatgaag tagccatttt agtgacttgc
     7861 gagtaagtaa ttgacagcta aagaggagga agcggaatga aactaaaaga cggattttac
     7921 tccagcggcc acggcatcgg cggtttaatg ctagatatgc cgacaaagaa tcctaaaaca
     7981 cgtaagaaac caaaattcaa agtcggtgac atggttcgct gcgaagcaga aggattcatc
     8041 tatccatttc gtggatatgt agaacacgtc tataatcact cagcaatcat tcgcattgaa
     8101 aacacgatgg aatgtgataa gtggttagcg aaaagcaaag agaatttagc agtggtgaga
     8161 ttggtggaca tggaagttat aaaatcttaa ttttgctttt ttacaactat cctacaacaa
     8221 tgagcgttat tccacaactg gaaggagaag aggcaattgt gaacatttta gagaatatag
     8281 atattaagca aacaaggaaa aatgctagac gattactaaa aagatacaga aatttagaac
     8341 gtttggtcgg accagtgaaa atagactttt ccgtgatgac tgttaccaaa aatttgaaat
     8401 tcacaattga cagtcaaaac gaagaaatca ttgaagcgat aagtactcga gattcggtta
     8461 tcgaagcatt aacacggcta agtagaatcc atttccaggt actttattat agttattgtt
     8521 ttcctaataa gatgtcaatg tatcaaatag gagaaaaatt aggatattcc gatagaacta
     8581 tcgagagaat gaaggcagta gctttggttg aatttgctga ggcgtataaa tcaggagaac
     8641 tcatttcacg tacaaaataa aaaaagtcgg attcctccga ctatgaataa tatttccgac
     8701 ataagtatta tatcataatt gggggaatca gaggatggta cttttcgatg taaagaaata
     8761 tgagacacca agcgcgaaag atgttgatat ggaacgcaca aaacataatg tcgctgtgtt
     8821 tctttcagca tatctatcag ctagatgtag agtagggcaa catcgagaac caaaagtgac
     8881 agcatcttat tccttggttc caccttctac agctgatcat atatttgaag cagaaagaat
     8941 gctgatcgat aaagaagaag cacaggaaga atttgagtat ctgcataaat tgtttgttcg
     9001 aggctattca gcgatacagc atccgcataa gcctgatgtg actgaaaggc gcaagaagat
     9061 attctatgat cgttatatca atggtctgcc catttatgta actgctcaaa ggaataatac
     9121 tagcgaagaa tcggttaaag tagaatcaaa cagaattatc atccaatttg cttcatcgtt
     9181 agaactggtt gctttcaagt agccagtttt tacacttttt ataccctttt attaccaatt
     9241 tggtttccat tttatacctt ttttatacca atcacttacc tattcaacgt tgtattatga
     9301 tagtgtcgaa aaattaggaa acaggatcga caaaataaaa tgtaagggag gaaatctccc
     9361 tcatcgttta attaagcttc gataggcagc aacggaaata ttaagaataa ggatgtgaat
     9421 ttcaactcct tctaaattgt tcttattatc tatcatccgt tgctgtctat tgttttttta
     9481 attattcaca cgataactaa aggtaagtga agagaaatga ttccattaat aatttcaatt
     9541 tttgcgcttt gtcttaatgt atatatgatt ggatttaaaa atgggcaaaa taaaaaatag
     9601 tagcagccaa gaataatttt atagtgtcac tgtggcggaa agggtagacg ctaagcatgt
     9661 gtgctaggtc aatgcttcgg caaccatgca aggttcgatt ccttgccagt gactttggca
     9721 accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg ttttgtgcat
     9781 cgtgtaggtt gctattacat aactggttag gttagattga gatttgggat tcggtacaaa
     9841 tgaatcgtca aatgtctcaa gcacaggatc ggaaacgtcc ctgcctgtgc attacatatt
     9901 agatcactct ttgagtggtc tttttcttta cataaaggag gctacataat gagaaactac
     9961 tggtatgtat cgctaactaa tgaatatcct cgaaccattg atgattgttc agtgcgtgtt
    10021 gtgcgttctg tacaaatcaa agggaagtac tctattgtcg aaatggggag agaagcagaa
    10081 ccatgtgaga ttgatgcgtg caagctcgtt tattgcggtc atggtttcta tgatgaacca
    10141 aacattcaaa ataatattaa caagaatttg agggattaga atgcaagaaa tggcttaaaa
    10201 atctccaatc aggacaaatc aagttagaca aagtttccga tttaaagata ttgattgaag
    10261 ctgatttaat gctaaaagat attgaaaatt agaaaacaaa actcaaccta aacagaatgt
    10321 gaggtggtgt gtattgaatg gcaagacaac gtgatccaag acgtgatgaa gccaaaagaa
    10381 tttggttaga atccaacgga gaaaagcagt taaaggaaat tgcatctgaa ttaaatgttt
    10441 cagattctca ggttagaaaa tggaaatcgc aagacaaatg gagcgctgaa ttgattagta
    10501 acgttaccaa tggcaaaagt aacgttacta atcaaggtgg cgctcctatt ggtaatcaaa
    10561 atgctaaagg taacaaagga aatagccgag cttctccgcc agtgggtaat aaaaacgctt
    10621 tgaaaacagg tgagtatgaa acaatatttt ttgacacgtt aaccgataat gagaaggaca
    10681 tctattctag tttgaatgat gatccttctt ttgctttgtc cgaagaaata cgacttctta
    10741 aaataagaca acttcgaatg atgaaaagaa tccaacaagc tgaagctgga ctaaatgatg
    10801 aagaagtcga acgattgcag cagctaagaa agattaaaac tccgattgaa aaaaatggta
    10861 aaaagctaga aatcaagcgt gaggttatgc aagatgtgca gattagcaga aaaaaacatc
    10921 gcaaaattga tgatattctt tcaattgaag attcattgac tcggattagc aaccagttag
    10981 ctaaagccat caagcaaatg aatgaacttt atatgaatga atacagaact gatttaatta
    11041 aagctcagac tgataagatc caagctgaga caaatgaaat tggcggaaat aattcaggtg
    11101 aagaaataga agaatggaaa caggcagttt taaatgccgc aaacaaacgg gcggtgaaag
    11161 aaaatgaata atgaatttat tccttttgct gatattggtt ctgccattga ttattactat
    11221 gataaaccag tagctttttg ccaagatatt ttgcatttga atcctgatga atggcaagaa
    11281 aatgttttaa atgatttagc tgaattttca aaggtttctg ttcgttctgg tcaaggagtt
    11341 ggaaaaacag cattagaagc aggagcaata ctttggttct taacgtgtcg accctacgct
    11401 aaagtaatag caacagctcc gacaatgaag caactttacg atgtactttg ggcagaggta
    11461 gctaaatggt taaatgatag cttgatcaaa aacttactga agtggacaaa gaccaaaatt
    11521 tatatggttg gtgattcaga gcgttggttt gctacggcta gaacagcgac taaaccagaa
    11581 agtatgcaag gttttcacga ggaccatatg ttgattgtgg tagatgaagc ttctggtgtg
    11641 tctgatccaa ttatggaagc tattcttggt acgctatcag gttttgataa taagctgttg
    11701 atgtgtggaa accccaataa tattgaaggt gtcttttatg attcccataa ttcagaccgt
    11761 gataaataca gagttcataa agtatcaagc tatgatagta aacgtacaaa caaagacaat
    11821 atagaaatga ttcttaaaaa atatggaaaa gaaagtgatg ttgctcgtgt ccgtattttt
    11881 ggagaatttc cgaaaggtgc gttggattca tttatcagtc ttgaaacagt tgaattggct
    11941 acagaaaaac aaattagtga ttctttagtc aataaaacaa aggttgctca tattggtgtc
    12001 gacgtagctc gatatggtga tgattctacg attctctttc ctagaattgc taccagggca
    12061 ttggagtatg agaagtattc aaaacgtagc accatggaaa caacaggata tgtcatcaac
    12121 atggccaaga atctaatgag tcaatatccg agtattgata aagtgatgat taaagtcgat
    12181 gacactggtg tcggaggtgg tgtaaccgac cgcctagaag aacttataga agacaaacat
    12241 tatccttttg aggtgtttgg agtgaataac ggttcaacat cagaagacga tttttacgat
    12301 aatttaggta ctcaactatg gggaaacatc aaggaaatgt tagaagaaaa tatgacagca
    12361 aatcttaacg gagaacagcc tgttattgaa ttgccttctg atagttcgtt aatcaaagaa
    12421 ttaagtactc gcaaattcaa aatgacaagt agaagtcgta tacgtttaga aagtaaagat
    12481 gatatgaaaa agcgaaatat tggtagtccc gatattgctg acgcactggc tttagcgttt
    12541 tatgagccac caagtcacta tcaatttatt caattttagg aggtgagctt ttgattagta
    12601 cagtattatc tttagagaga tataagaagc tacgtgttaa atatgcaaca caaattgaag
    12661 atggaatgtt tgatccgaat ggttttatag aagatatgaa accatttttt gctgatcgcg
    12721 aaagaaaata tctagcttat actagcgaaa agaatgaaat agataataga ccaaagccta
    12781 acactgatat tgtaaaagtt aataataaac ttcatgctgg tatgtattca attgtcgtag
    12841 accaagcagt caatcatttt actggtatac ctatcaaatg ggattacgat gtatcggaac
    12901 aaaaaagaac actcattcaa agattaaaag ataaattctt aaaaaatgat atagaacttc
    12961 cgacagttcc agaagctttt aacaaattaa caagcaatct tgattccatg agattcgcaa
    13021 tgcttgattc tgaaactgca acttttcaag gagcttgtgg agtagctttc cgattgctag
    13081 agcctgtgga agaagatgat ggttggaagt taagagcaag taatattgaa ccttggagag
    13141 cagaaagata tggaaatgcc ggaatctata tcaaagaaaa gtatgactct taccagaaaa
    13201 aattttttca agaaatgaaa gttatgacaa gaaataaaat tctgacatat gcttgctatg
    13261 gtgatttgaa ttttgttaca agcggaacgt tcaaaaaaat tgatgagacc gataaccctt
    13321 tggggacgat taccttggca gaatttaaaa ataatacgaa tcgttattgt gattttgaag
    13381 tagctgagga aattggagat gcaattgatc gtgctttgtc tgatcaacaa aacgaaatcg
    13441 aacaatttaa gcttgcgtac atgcttgtta ctggcacaac aatgagcaaa ggcactgcta
    13501 aagaaatgat gaatcagcta gggattatca acttaaaagc ccctactgca aaagctgaat
    13561 atgtcacaaa aaatttagca aaagatttca atgaatatca tatggatcta ttgaaaaaac
    13621 agttttacac catttgtaaa gcaatcgatt ttaacgatga ggtgtttaaa tcaaacagtt
    13681 ctggagaagc tcgcaagtgg caaatcatta gcctagaagc gaaaacaaat actaaagagc
    13741 aatattttag agaaggattg aaagaatgcg cagaaacaat tgctgctttt cttaagtttc
    13801 atgacaaagt agaaattgag cctgaaaaaa ttatttttac tttctctaga tctttgccaa
    13861 ctgatttaag ttatttagct gaagctttac ctaaacttgc accatacgta tccaaacgaa
    13921 ctattcagag ccaaattcca tttgtaacag atgttgatta tgaaaatgaa atgatggaat
    13981 tggaaagtgg aagtgcttat ccagatagtg aatacaattt tggcggaggt ggcaatagtg
    14041 acgatagaaa cgaagtattg gaccaaacgt cgagaactgg aggatcaagc aaggctcaaa
    14101 caagaaaatc agacacttaa aaaattaact agtgtatttc ctgaagcact gaaagagata
    14161 caagcaaaac tattatcaca agctgactta cacaatatca cttatccaga aatgatggag
    14221 ttttacagta caagtaatca gaaaaaatat cgtgaatatg tggaaaaaaa ttataagtca
    14281 ttaaaaatgt atgatgcaaa atacaaagag tttatcgatg aattttttcc accatttgac
    14341 tatgcaaaag tcaatcgctt attacaaata cgatcagatg tatttaaaat tcttgcagaa
    14401 tatgcgatgg atgcagatgt gaatcaatat ttttctgatc gcttagagga aattcttcaa
    14461 agaacatatt cttctaatgc taatgttttt gttcaacttt taaacgttga tataccaaat
    14521 tatttaccag aaaatgaaat taaacattat ttgaactatc catggtcagg aaagacattt
    14581 tcaagaagac tttggggaaa tatttcatcg cttgaacaga aactctctaa tgctattgta
    14641 aaaagtgttg ctagtggaga aggcgttata cacgcattaa acaccatgag attagactca
    14701 gaaatttgtg acatgtttaa gttagaagaa tcaaagtata acaaagcgat agaaaatctc
    14761 gttcgaacgg agtatgcaaa atttgcacaa gatggtattg aaaaatcata tttagaaaca
    14821 ggtattgagg aatacaacgt attgactgca aaagatgaga gagtttgccg gatttgtgga
    14881 ggaaaggcaa gtaagaatcc ctataaactg aaagatgctg tcataggtga aaatcgagca
    14941 cctttccata gtcgttgtag atgtacggat gttcctaatt taccaaaatt aggaaaggat
    15001 attgatgaag aatatgaccg tttatttggc gatttattag atgagtttgc acatgattct
    15061 tttggaatta atttgaaacg gaggaagtag aatgaaagat ttttttgaag cagtactaac
    15121 aattaatgta aatgctgata ttgcagaagc ctacaaaaca gctattgagt ctgagaacca
    15181 tcctaatggc ttgagagacc attggaatgg caattatgcc tacgtggtta ttggcgatca
    15241 aactgttaat tatcaagata atactccagt tgataagaat accgttaatt taacgattca
    15301 attattatct cattcattac caaatttaaa agaaacagtt gattggtatg aaaagatggg
    15361 atgtattgtt gttagaactg actacaaaga aggaaagtct agtaattagg cttttttatt
    15421 ttgtccgaaa tgacgttaaa ctagcgcaat actgggcttg gttgaatggt ggggcgcaac
    15481 tattaaaact caaagcaatg cggggcgtga aaacgaatcg tggggcgaaa ggagaatgag
    15541 catgaaacac aaatcattaa tgccaatgaa tttgcaatac tttgccgaag gtgacgatca
    15601 aaagttttct tttgatgact tcaaatcttt tgtggaatcc aatgaagaag ctcaaaaatt
    15661 tattcagtca caatctcaaa gtgttgccga caaacaattg gaagcttgga aacaaaataa
    15721 cttagataaa attaaacagg ataccatcaa ggaatatgag gaatctaaga aaaacaagtc
    15781 acctgagcaa attcaactgg aaaaactaca agctgaattt gaagcagaaa aagcgttgcg
    15841 tgtgacaagt gataataaag catttgttgc agaacaaatt gctggattag aactagatgg
    15901 agagttaaaa gagtctattt ctcaatttat gctaaataat cttgttagtt cggatacaga
    15961 tttcactaag aatgctgttg aaggttttac aagtgttttg aatgcaatta atgagaaaca
    16021 tgcagatgca ttaaaagaac tacaaatgaa gtctgcattt ggtggaactc aacaatcgaa
    16081 taaccaggtt cagcagaaca atgaaacatt aacaaatcca gaagaacaat taggacaaat
    16141 tcttcaacaa tttaactagg agagtgaaaa attatgaaaa aaacatcttt aaataattta
    16201 gagtatttgg atatttcacc agcgattaat gctatgcaag taccaaatac acctttttta
    16261 agctatttac ttggtgctgg aaaaacagag ccagcaaact cgacagaaat taaatggcgt
    16321 gaatatgata tcaacaacga tgattcttct gaaaaacttg agggcggaga atatccagat
    16381 gctgaatcag gtcgaacttg gtttaacaac tatactgaaa tttttagaaa atcaacctct
    16441 gtatctggta cattagatgc tattaatgtg aatggagtcg gaaatgaatt aactaatcaa
    16501 gtagcactac gtggtatgga aatgaaaatt gatttgaacc gaaaattgat tactggtgta
    16561 aaagctgatg aaaatggttc taaaggtcgt cgaatgaatg ggattttgaa cttgatcaat
    16621 tcagcaaata aggcagaaac agccactgcg ggtgcagtaa caagaaaaga tatcgatact
    16681 ttatttaaat tgatgtatga aaaaggttat atgggagaaa aactatgctt gatttctcca
    16741 gatatgcagg agttaatgac tgatgagtta gatggaaaat caacaaaaat tgttcagttc
    16801 ggggaaagag taacttttgg attgcaaatt ggaaatatcg tgtctaatta cggtacaggt
    16861 attgctctac tagaaccatc attgccaaaa ggaacaattg ccgcaatcga tactaattat
    16921 gtgaaactac gtccattacg tgaatggaga gcagaagaac ttgcaaaaac aacggattct
    16981 agacgtatcg gtcttgtagg tgaatactct cttgaataca atgcttcaaa ctctggggca
    17041 attttaaatt taaagtctga ataaaaggga gttagtactc ccttttttga taggaggaat
    17101 tacagtggta aaaaaagatg aaactaaaaa agatgaagtc gtgaaatata gagtaggtaa
    17161 aactaaaaat tttgttgggt ttgttcatcc taaaactcgt agatttatca cagcagattc
    17221 aaataacgaa tttatcattt ctatagatga taaagaagca attgcaattt tggaagatgc
    17281 aattgatgtt aatgaaattt aggaagtgat ctgatggatg aatcgctaaa aacggaaatc
    17341 attgagtcta caaaagaaga ttttccagat ttgagtgaag aacgcataac taatttatta
    17401 gaaataattt tgctagaaat tgaatcatac aacacttgta aaaatgatat ttcatgggaa
    17461 aagttaaaaa gcgtgattag tgaagtgttg tataaaataa taaaaaatga atcagaaaaa
    17521 acagtatctt cagttagacg tggcgataca acgattagtt atgcttcaac aacaaatgat
    17581 gttagtgagt tacttctagg ctatggtgat ttgatacgaa gagttattgg ttgtggagga
    17641 ttggagtttt tttaatgaat gaagcagata ttttagaaac tacttatgaa gatagttgta
    17701 ttattgaaag actgacggac attgaagatt ctaatacaaa tattactatt caagattaca
    17761 aaaaagtata tgataatcct atttcttgcg ctctttcaca aggtcaaatc gatggactag
    17821 cagtcataga agatggagag atggtaaatg tttcaactga cacatataaa ttatttgttc
    17881 atcctaagat taaactaaaa aaaggagatc gaataacaat aactcaaaag gccagtggct
    17941 taattttttc tctatttgct actaagcctt tttactatcc tagtcattgt gaagtgaatt
    18001 tgataggaag tgagaaaaat gggtaatctc aaatatgaat caaatgcaga aaagatcatt
    18061 gagaatttta aaaatatgac tgtaattgct caaaaagaag gaatatcctt cgttaacgat
    18121 tcaatgaata aagtcgttag tttagctaaa ccattaactc ctgtaaaatc aggtaattta
    18181 aggcgaggat atcgtgtagt aaaagctaga aaactatcaa gcggtcgaat tgttggagaa
    18241 gttataaaca atgaacatta ttttaaatat gtagaagaag gtcataggac taaaaatggt
    18301 ggctttgtca aagggaaatt catgttaact cgtgcaacga atcttgcaaa tatgtcttat
    18361 attcctcgaa gatttaaaca aatggcaata aaaatcgtta agaaaggaaa gtaacatgaa
    18421 agatgaaatc attgctgcaa tcagtagcaa attaaaagaa atctatcctg atgggacaat
    18481 atatcttgat tcagttatgc agtcaactaa agatttttat ttcgttttat cagtaatgga
    18541 atctggaact gaaaatgtag gaattgatat tcaaaatgtt tctttcttaa ttgatattgc
    18601 attgattgat aataacccta atagaaattt aattaatgaa ttagtctcac gttgtgggac
    18661 tttttttaat acgattacaa tcgatgagca aatattattt ccaaaagcct atttacctga
    18721 tgaagcagat ggtgttcaac atattcgttt tacattagaa tttccacaat atattgaatg
    18781 gagtgaaaga taaatgggag aaaaaagaag taaaaccgga attatttctg tcgaaaaacc
    18841 tacttggttt cctttggaag atgagacagg gaattttccg gtatatggta ctgcgttgcc
    18901 aatgggaaca gcagtaagta tcaaaccaac agctaattat gaaacaacac aagattatgg
    18961 ggattcagtg gttcaagatc aatttacagc ttttggtggt gcagaagttg aactggaagc
    19021 aaatggatat actcataaag ttttatctac aattactgga gggaaaattg ttaaaggtgg
    19081 tgccttgcgt tctggagaag atattgcgca agatggtgcg tttgcatata gacgtaaaaa
    19141 atcgaatggg aaataccgtt atacggtctt ttataaagga cagtttgctc tagattcaga
    19201 tgaaacatca acgattgaag gaagtaaagt aagttttact catccaactt ggaagggttc
    19261 atttgtagat gtaccaggac ttggatatat gtattcagtt gatgaagatg atgaaggcgt
    19321 ggataaagca atgatcggaa attggtttac aaaagtagca attccaattg aagaagcaga
    19381 actttcagga ggtacagaat aatgtcaaaa tatcaaacaa cgattaaatt aacgaaaaaa
    19441 gacgaagaag gtaaatatga gcaagtacaa tttaaatctg ctgaattttt accaggaaca
    19501 gttgtagaag atgctgcagg agttatggaa gaaatgcaaa cagcgactga taagcaatca
    19561 gttaaaaaag ctttaagtcg tgcttattca tttattgcag atactttatt tgagggacaa
    19621 tttactggtg aagattattg caaaggaatt gatgctcgtg agattgcctc attgacagga
    19681 aaattattga agtctgttac tgcaggtttt gatgaaactt atacagagac gaaaaaaaag
    19741 taagtgaggc gctcaagtca ccttcattta agtattcgat tacttaccga gaattagata
    19801 taaagacgca attactcgaa gcaggttgga cgttaccaga aattgaacgt actgacttgg
    19861 atgagttaat gcgtcttttt gcttttagag atgcggtaaa agagcatgaa gatgttgagt
    19921 actatgataa tttcactcaa ttttaggagg tgatactttg aataacgatg acctaattct
    19981 gaagatgata ctagatgaat ctggttttac tgccggtatg aataatgctg tcaaaaagtt
    20041 gaactctttt gatgaaacaa tcgaaagaaa aagtagaaat agtggcagtt cattaggtag
    20101 tatttggaaa atatttgctg gaagttttct agctagcgga gtaactagaa tcgtaggagc
    20161 tggctttgat ctaatcaagg gttccataag tggagcggtt gatcgagtag atacgatgaa
    20221 taacgctcta cgaaatttcc aaaatatggg atttagtaat tcagaaatta tgaagaatat
    20281 cggaaagaat gggcttttat ctcaaggtat tcaaggactt cctactgctt tgaatgatgc
    20341 gataagtcat gttcaacttc ttgcttcttc tacaggtgat ttaactcgtt caactcaaat
    20401 atttaaagct ttaaatgatg gaattcttgg tttcggtggt tcaactgatc aagttaatga
    20461 agctgttatt caattatctc aaagtttctc aaacggaaaa gtagacgcac aaacttggaa
    20521 ctcaatgatc aacgctcaac ttggacctac tctttctgct attgctaaaa agatgggaat
    20581 tacaatggga gatctgaaag aaggtttatc tcaaggtaag atttctgttg aagaattcca
    20641 aaatcaatta atagaaatgg ataccaaagg tggcggagga cttaaatcat taagtcaaat
    20701 cgctaaagat tctactaagg gaattaaaac ctctatacaa aatgcaaaaa cagctgttac
    20761 acgtggtgtt ggtgaggtaa tagaaggatt aaataaagca ttagtagatt cagatttagg
    20821 tggatttaag ggaattattg ataaagtggg cagttcaatg gaatcgttct taaaagtaat
    20881 tgctgcaaat attcctacag cagtatcatt tttaagtaat ttatttaacg aaattcaaaa
    20941 atttggttct gctttgaaat tcatgatgcc ctttcttgtt cctgcagcta ccgcttttgg
    21001 agcgttcatg tttcaactta aaggtatacc agcaataata aaaagcttca ataattttaa
    21061 gaatgccata attggcgttg gaaattcatt aaagattatg ggggcaatag ctgctgctaa
    21121 tccatttgtt ttaattattg gtgcagtcgt tggtgcaata gctgttttcg gttactttat
    21181 ggcaaccaat gaagagctta gaaacaaggt tatatctatt tggaatgatg tgaaagattc
    21241 cgtaattggt gtattaaaaa atataaagga ctggggaatt gatacttgga attctgctaa
    21301 agaaatggca tcaaatgcag ttgagggtgt caaagatgct tggtcaggaa caaaggaatg
    21361 gttttctaac acctggaaag gtattaagga tggggccaca ggtttatttg ataaaacagt
    21421 agagacttca agaaatgcag tcgatagtgt aaaaaatgca tggtctaata tgaagaaatg
    21481 gttttctgat acttggcaaa gtataaaaga ttcagccaga gaaaaatgga atggaatcaa
    21541 aggttctatt atgtcagttg ctggtccatt gattacaggt ataaagaatg catttttaca
    21601 tgttactttt tatctagaga ccttatggaa taatttagtt gagattggta aaaacgtctt
    21661 tgagatttta aaaaatgtta tacttgcgcc ggttttgttt attacctcca tgatttctgg
    21721 tggatgggaa gaaacaaaaa ataatatgat tgctgtttgg aataatatta aagaaagtgc
    21781 cata
//
LOCUS       DAZKMB010000042        21329 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000042 DAZKMB010000000
VERSION     DAZKMB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.042"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..1330)
                     /locus_tag="ACG8V3_001375"
     CDS             complement(68..1330)
                     /locus_tag="ACG8V3_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0299922.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1323..2222)
                     /locus_tag="ACG8V3_001376"
     CDS             complement(1323..2222)
                     /locus_tag="ACG8V3_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299923.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2367..4145)
                     /gene="uvrC"
                     /locus_tag="ACG8V3_001377"
     CDS             complement(2367..4145)
                     /gene="uvrC"
                     /locus_tag="ACG8V3_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0299924.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4327..4641)
                     /gene="trxA"
                     /locus_tag="ACG8V3_001378"
     CDS             complement(4327..4641)
                     /gene="trxA"
                     /locus_tag="ACG8V3_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0299925.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4728..7088)
                     /locus_tag="ACG8V3_001379"
     CDS             complement(4728..7088)
                     /locus_tag="ACG8V3_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0299926.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7203..7751)
                     /locus_tag="ACG8V3_001380"
     CDS             complement(7203..7751)
                     /locus_tag="ACG8V3_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0299927.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7809..8246)
                     /gene="zapA"
                     /locus_tag="ACG8V3_001381"
     CDS             complement(7809..8246)
                     /gene="zapA"
                     /locus_tag="ACG8V3_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0299928.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8448..9365
                     /gene="rnhC"
                     /locus_tag="ACG8V3_001382"
     CDS             8448..9365
                     /gene="rnhC"
                     /locus_tag="ACG8V3_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0299929.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9743..10654)
                     /locus_tag="ACG8V3_001383"
     CDS             complement(9743..10654)
                     /locus_tag="ACG8V3_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0299930.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11055..11633)
                     /locus_tag="ACG8V3_001384"
     CDS             complement(11055..11633)
                     /locus_tag="ACG8V3_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0299931.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11653..12321)
                     /locus_tag="ACG8V3_001385"
     CDS             complement(11653..12321)
                     /locus_tag="ACG8V3_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299932.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12336..13406)
                     /locus_tag="ACG8V3_001386"
     CDS             complement(12336..13406)
                     /locus_tag="ACG8V3_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0299933.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13577..14941)
                     /locus_tag="ACG8V3_001387"
     CDS             complement(13577..14941)
                     /locus_tag="ACG8V3_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0299934.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14938..16497)
                     /locus_tag="ACG8V3_001388"
     CDS             complement(14938..16497)
                     /locus_tag="ACG8V3_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0299935.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16553..19033)
                     /locus_tag="ACG8V3_001389"
     CDS             complement(16553..19033)
                     /locus_tag="ACG8V3_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299936.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(19017..19352)
                     /locus_tag="ACG8V3_001390"
     CDS             complement(19017..19352)
                     /locus_tag="ACG8V3_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299937.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19366..20400)
                     /locus_tag="ACG8V3_001391"
     CDS             complement(19366..20400)
                     /locus_tag="ACG8V3_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0299938.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20490..21170)
                     /locus_tag="ACG8V3_001392"
     CDS             complement(20490..21170)
                     /locus_tag="ACG8V3_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0299939.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         6084 a         4458 c         3646 g         7141 t
ORIGIN      
        1 ataggtctat acaaaaagaa gctgggaaca atagatgtcc agcttccttc tgtattttag
       61 gattgtttta tgcttttttt cgttcattcc tcataattga aatcgattgt ttcaatgaac
      121 tccataatcc gccttcgcta taaacgagta cattcgattt gtacatgttt gcagacaaga
      181 tcatcaataa gaatgttacg gctacaagta ccaacaagga aatcaatgct ccacccatac
      241 cgactgtctc atttgctaag cgaatcggca taatatatga agataagaat ggaatatacg
      301 aagtgatccg aataacgata ttgtttggat ctgaagctcc tagtatcaat ccaagcatgt
      361 atccgcctag agatagataa gtcactggca aaattgcttt tgctgtatct tctgctttgt
      421 tgaccaaaga gccacataaa gctgctaaaa cagaaaaaat caaaatgcca aagatcatga
      481 atagaagaga ataccacaag aatggtccaa tcaagttatc caatgagata ccgtctaata
      541 tatttttgac catttccata tctttgaatt gactgtatcc tataccaaaa ataataccgt
      601 ataatgccat ttgcgttaat gcaacaagta aaacacctgt taattttccg taaaaatgct
      661 tttgtgcagt cgtactggac aggatcactt ccatgatccg cgtgcctttt tcagaagcga
      721 tctcttgtgc aatgatctgc gcatacgtca aaataataat gaacaagaca atcgttgcta
      781 cataacttac gacgtattgg actgtcgagt tatcttctcc gactgtcatt tttccttttg
      841 aatcaaaatt gactttttgt cgagtcaaac ttgcttgttg gctcaagcta gctacttctt
      901 ctggtgaaat gcctaattga ctcgctcgaa gcatcgattg caagccagtc agttgttgtt
      961 gtatcagtag ttgtgtactt tgtcctaatg aattttcgct ataaagcatc ccatttatct
     1021 tatcattctc agttgagact actaaatacg catcgatttt ttcttccgaa agctcttttt
     1081 gtgctttttc ttcagaatca actgctttga actcaaaatc atctgttttg acttggctca
     1141 tttgctgact gatttgctca ttatcagcca atacgccgat tttattgaca tcactatttt
     1201 gttgagcaaa gtttcctgct acataaatga tacccagcat gataaaaggg actaatatca
     1261 tgatcaagaa agaaatcgac ttaacatttt ttaaatagac atctttggta ataatccaaa
     1321 acttacgcat cgatttcacc tgctttcaat ttgaaaatct cttccaaagt tggcggttgc
     1381 tggttaaaca taggaatgta gccgaactga gtcgcacgag taaagatttc tttcccagct
     1441 tcaggatctt ctaaggtaat ctctaacgta ttgtcttcat gaaggatcac ttttttcacg
     1501 ccttcgattt cttctacttc ctgttggctt aaaccagatt ccaagaataa cttcgtacgg
     1561 ccaaatgatt cacgaatctc atgtactttt ccattcaaaa ccatttttcc gtttcgcaac
     1621 atgatcaaat gatcgcagat tttttctaca ttatccatat tgtggctaga aaaaatgaca
     1681 catgaaccat gttttttcaa ttccaatatt ccatctttta gcaattcagc atttaccgga
     1741 tctaacccgc tgaatggctc atccaaaatg atcaatttag gttcatggat taacgtggcg
     1801 atcagctgga ctttttgctg atttccttta gacaaagatt ttactttatc cgtctttttc
     1861 ccttttacct gaaatttttc catccaaaag tcgatttttg gttcaatctc ttttcttgat
     1921 ttccctctaa gttctgcaaa ataaagcagt tggtcttgaa tcgttacttt cggataaagt
     1981 ccccgttcct ccggaagata accgatgaca tcataatctt tctccccaag atgatggcca
     2041 ttccataaca cttctccttg gtcttgtgtc aaaaaatcca agatcaaacg aaaagtcgtt
     2101 gtcttcccag ccccgttttg gccaattagc cccatgattt ttccatcggg gatttggaaa
     2161 gagacatgat cgacagctgt gtagttccca aaagttttaa caagatcctt gacttctaac
     2221 atttcttcac tccttcttca agaaatcaca tatatcctta gacaccagac gtaagcgatt
     2281 tattatttac caacaaaacg taaacgctct ttttgttgta cataaaatga taggacactt
     2341 tactttcttt gtaaagtgcc ctttcctcat tcctgatgta atttatcaaa tacgttttgt
     2401 gcgacatttt tcggcagtcc gatttcttgt aattcttctg tcgtggctgc tgtgatattt
     2461 ttcaacgatt tgaactcttt aagcagcatt ttttttcgtt ttggtcctaa cccttcaatt
     2521 tcatctaatc gggaagcaaa actgttttta ctgcgtagct gccgatggaa agtgattgcg
     2581 aatcgatgga cttcatcttg gattcgctgg agtaagaaaa actctggtga atttcgctca
     2641 agcggtacta cagccaaatc tgtaccgaat agcaactcat ttgttttatg tttatcgttt
     2701 tttaccatgc cagcaatagg aatatcgatt cctaattgat tttctaagac atcttttgcc
     2761 acatctactt gtccttttcc tccatcgatc aagatcaaat caggtaaagg caacccttcg
     2821 cgaatcactc gcgaatatct gcgatagatg acttctctca ttgatgcata atcatccggt
     2881 ccgttgactg tttttatttt atatttccga tattcttttc ttgccggttt tccatcaatg
     2941 aatacgacca tcgcagccac aggattcgtc cccatgatat tagagttatc aaaagactcg
     3001 atacgtattg gggtcgggat attcatcgcg tttcctaaac gttcaactgc tccgatcgtc
     3061 cgttcctgtt ttctggcaat cagatcaaac cgttcattca aagcaactga ggcattctta
     3121 ctcgccaatt caactaattt tttcttttct cctcgttttg gttgcagcac tttcgtagct
     3181 aacaaagctt ctacactggg cttgtcgatc gtatcaggaa tcaagacttc tttaggaatg
     3241 aaatgctcat tttcctgata aaactgaccg aggtaagtca agaaatcttc ttctgcttcg
     3301 ttatagaaag gaaacattga aacatctcgt tcaattagct ttccttgtct tacgaaaaac
     3361 acttggacac acatccatcc tttatcaatc gcataaccaa atacatctcg atccagtaaa
     3421 tcagtatttg tcattttttg gcaagtcatg attgtctcaa tggcacggat ctggtctcgg
     3481 tattctgcag ctttttcaaa ctccatgttt tcagcagctt tttccatttt ctgatggatc
     3541 tctgactcga ttttttcgtg tcctccgttt aaaaagtttt tcacttcatc aacgattttc
     3601 gtatagactt tcggatctac gtcgaaataa taaggacaca ggcattgtcc caaatgatag
     3661 taaagacaag gctccttcgt ctgactaggt ttgcattttc tcagaggaaa taatcgatcg
     3721 aggatcttct ttgtctcatt cgccgcaccg acatcgggat atggtccaaa atacagtgct
     3781 ttgtctttta gtaccttacg agtaatcacc aaacggggat atttttcatt cgttatctta
     3841 aggaaaggat aggttttatc atcctttaac ataatgttgt atttaggatc atttttcttt
     3901 atcagattga tctctaagag caatgcttcg atattcgatt ctgtaacgat gtattcaaag
     3961 tcttcgattt cactaacaag ccgttccgtt ttcgtattat ggctaccagt aaaatatgag
     4021 cggacacgat tttttaatat tttggctttg ccgacataaa tgatcgttcc atttttatct
     4081 ttcatcaagt aacagccggg ctgatcagga agtaaagcta atttgttttt gattcgttcg
     4141 ttcatctggt actccccttc atattcaatt aaagttatta tagcatgtct gtcaaaaaaa
     4201 caatccagcc tgacaacgta attatactca aaaaatatta atttgaataa ccttaactca
     4261 accgcaaaaa aaccgaggac ggaactggat tcagtcccac cctcagctta tttttgctgt
     4321 aagtttttat aaatattgat cgatcatttg acgtagttgt tcttttgaat gaacaccaac
     4381 agctttttct actacttgac catctttttt caacattaat gttggaatgc tcatgatacc
     4441 gaattgttga ggagtttctg gattttcatc cacatccatt ttagcaattc tgaattcttc
     4501 ttcatcatat tcttgttcca actgatccaa aatcggtgct tgcatacgac atggaccaca
     4561 ccaagttgcc caaaaatcaa tcaatacaag tcctttatct gtttcttcat taaatgtctt
     4621 atctgtaatt acttgtgaca tatcaaagac ctccattttt aaaatctatg actagtatag
     4681 catttctata cttggatagc gaaccatctg cttaccttta gtaagcttta cttaaattta
     4741 acaattgtcg caccatttcc gccttggttt gccggtgcaa actcaaagct tttgacgctc
     4801 cgatggtttt taagataatc agtaattccc ttgcgaagtg cgcctgttcc ttttccgtgg
     4861 acaatagtca cttgaggata accagctaga atggcagaat ccaaatattg atctacctca
     4921 cttagcgctt cttcgtatcg ttttcctcga aggtccaatt gattcggcac gtgactacta
     4981 gattcagatc gtacagcatg gataactcgt tgtgtcggct ctttttgagg agcaacaggc
     5041 gtcagctcgt cttcggacac attcattttt aatataccga tttctacttg ccactgattc
     5101 ccgtttttac gtaaaagcgt ccctctttgg ccgtaagtcg taaccaacac ttcgtctccc
     5161 ggtttcaacg ttttttgttc tttcgccttc ttcaatactt tatttttctt caaagtttct
     5221 tcttgatgta gatttgctaa ttgggtcttt gcatcgatca actgatgctc tttcacattt
     5281 ccttgcccaa tctgctgttg cattttacga atatcagcaa tgattttttc tgctttttct
     5341 tccgcctctg acacgacttc atttgctttt ttctttgctt tttccatttc tttttcacgt
     5401 tcttcaaaga aatagctgta agcttctttc aaatcatcgt gaagttcttg tgcctcagaa
     5461 acaaaatgac gcatttccag atattcggtc tctgccattt tccggcgatt ctctaaatct
     5521 gtgatcattt cgtttaaatc ttggctctca tcattcatca actgcttcgc ttcatcaata
     5581 acttctgtat caagacctaa tcggctagag atttcaaacg cattgcttcg tccaggaacg
     5641 ccaatcagca aacgatacgt tggacttaat gtatccacat caaactccat gctggcatta
     5701 atcgtatttg cccgattata tccataaact ttcaattcag gataatgcgt tgttgccatc
     5761 acataagcag actttttacc aagatcatct aagattgcga ttgctagagc agcaccttct
     5821 tgcggatctg ttcctgcacc gagctcgtca aataatacca gacttttttc attgactttt
     5881 gataaaatat ctacagtgtt cgtcatatga gaagaaaagg tcgatagact ttgctcaatc
     5941 gactgttcgt ctcctatatc cgcaaatact tcctcaaaaa taccgatttg actttcttca
     6001 cctgcaggaa tcggtaatcc tgcttgcccc atcagttgta acagacctaa agtcttcaac
     6061 gtgatcgttt tcccacctgt gttaggtcct gtaatgacga tggcttgata gtcttttccg
     6121 attgtgatgt cattagaaac tactttttcc tgatcgatca gtggatgtct tgcttgtttc
     6181 aagacgacat gattgtcttc actgattccc ggcacgatgg cttttacttc tttaccaaat
     6241 cgtgcttttg cattcatcag atccatttta ccaatcacat aagtattgtg gatgatttct
     6301 tgacgatggg gaacaagttc agcagaaagt tcacttaaaa tacgttgaat ctcatttcgt
     6361 tcagcgattt ggtattgacg caaacgatta ttcaaatcca ccacttgttt tggttcaata
     6421 aataaagtct gacctgaagc actttgatcg tggactacac cgccaaatac accgcggtat
     6481 tcttgtttga ctgggataac gtagcggtca tttcgcatgg tgatgatcgc atcacttaaa
     6541 tactttgcat ttttcccacg gacgatccca tctaactgtt cacgaacagc ttgttcactt
     6601 cgccgaatgt tctgtcttat gctctttaac tcaggagaag cgtcatcagt cacgcgtccg
     6661 tcttcatcga ttgcttcctt taaacggcgg ctcaaaacag ggatcgtgac taactgatct
     6721 gcccacatat acaaacgtgc aaattcgatt tcactgtcag aaagatcatc aatgaaacga
     6781 ttcaattcgc ttgtcgtaga caatactctg gaaacttgtg caagctccac tccattcaag
     6841 tctgctccga tctcaatccg tttcatatgg ggacggatat tttcaatttt aggtataggg
     6901 atcccgcctc gcagacgttg cactttcaat ccatcctcag tttcttgaag ccagtttgtg
     6961 atcgtttgtt tctcactcac tggtaccaat tctgccagtt cttcttttcc ttgtgcagtc
     7021 actacaaatt gtctgaccat ctgtttgact ttttcaaatt ccaatgtttc aaggattcgg
     7081 ttattcattt tatcacctta tctttcacta tgaaaaaaga gctgtgccgg acatacgcta
     7141 cgcagccttc ttaaataaac tactctgtac aaaaaacgaa atattttcca gcactatttt
     7201 ccttaattca aaatattcgt tacccaaagc tcatacatct tgttggtaag aaatggcgtt
     7261 ctctcaacga taaaacgagc gaaactatgc tcgccgaatt gattttggac cacatctaat
     7321 ggaacaaagg caagtaactg caaaatcaaa aatatagtaa cgtaagccat gattagtgac
     7381 aggatgcctc ctgcaatcca gtctacctgt ctcaagatcg gcacatacgt caatccatgg
     7441 acaaaaaccc ccacaaatcg ggtaagcagc cagccaatta gcaaaataat cagaaaagca
     7501 gttcctgcat aaaaagcttc atctaggtga aaggcaaata cctgatcaaa aaatgctaac
     7561 tttgatgttg gggtgacagc tgggtatgga atgtataatt ccaaatgact tgctaatggt
     7621 ttgtaaaaat gctgggcagc aataaatgac aatacatatc caatcgcgaa tatgatttgc
     7681 aaagcaaacc cgcgacgtgc tccagaaaaa aacgcaatta gcaataaaaa taaaatcagc
     7741 aaactcagca tgctacgttc ctttctcttc taactttttt aaatttttct tattttgata
     7801 aaaggatctt aattagaaga tttttgttta atttgtcctt ttcgtttctc gtttaaaatc
     7861 tgttgtgctt ccacatgatt tttaatggta tgatctgctt gaccattttc tttcaatact
     7921 tcccgagctt cgttctcgat tgcttcaatg cgtttcacac gattttcaag ctctgtaaat
     7981 ttgatcattt ttttctttaa ttctgctgtt tcttcttcca gctccaatat tctttcttgt
     8041 ttcttcaatt gatccgaaag cgcattgaca gccatcaaga ttgctgcttg ttcattatcc
     8101 atttgtggtg ataattgttt caattctgct aactgttcat tgatcagctt cgttacgata
     8161 tccatatgat gcttggtttc ccgtccgata atagtatatg tttggttggc aatcaccgct
     8221 ttgtagcgtg ttttttcgtg cgccatacga atcatttcct cctatttgat gaaagtttta
     8281 tttagtgtac catgaagttg gttctttcac cgttctccac aaatattttt cacatactta
     8341 actattatat gataaacttt aaaaaaatgc tatgtctcgc aatctcttag aaaacgaaaa
     8401 gtcgtgagtt gcggtatagt tttatgtata aaaacgagga gaattttatg agcaacgtca
     8461 ttatcaaagc aacaaacgac caacttagtc aaatgaaagc atactatcgc tcttatttgt
     8521 taaataaaca gattccttat actagctttg ctgcaaaaaa agacgggacg accattaccg
     8581 cttatacatc cggaaaagtg atgtttcaag gtacgaatgc agcaatagaa gctgataaat
     8641 ggggggaggc acaggataaa caagccaagc cagcaaaaac agcctcttct ttgccaaaaa
     8701 atatcagtca attatccgtt ttaggtagtg atgaggtcgg aaatggcagc tatttcggtc
     8761 cgctaacagt atgtgctgct tatgtgaaac gtgatcagct tgcagaatta cgtaaacttg
     8821 gtgttagaga ttcaaaagag ttaaaagata gccaaatcat ccagcttgct gacatcttaa
     8881 aaaaaaccat cccctataaa ctgcttgtct tatctccaga aaaatacaat gagatccaac
     8941 caaaatacaa tgccgttcgg atgaaagtcg ctttgcacaa tcaagcgatc catttgctgc
     9001 taaaaaaaat cgagccagaa aaaccagaag cgattttgat cgatcagttt acgccggaag
     9061 caaattttaa aaaatatgcg cgtttagaaa aaaatcaatt acaagaaaaa ctgtacttcg
     9121 ttactaaagg agaacaatat catttagctg ttgctgctgc ttctatcatc agtcgtgcca
     9181 gctttttgga agagttagac aaagcatcag ctgaagctgg aatcaccctt ccgtcaggag
     9241 ctggaacgaa atctgaccaa gtagcggcaa aactattgga gaaaggcggc atgcctatgc
     9301 ttgaaaaata tgccaaactt cattttgcca atacagaaaa agctttgaag ctactaaaaa
     9361 aataatggaa ctgtgacaaa agtcatgtca cagtcccttg ttccgaataa acggtgttca
     9421 atcatctgat cctcggtatt agctcaacaa acggcaaaat atgaggaact atttttccgt
     9481 ttgtttctct aatactcaga acaaaacgtt gatttcacaa cctttttctt aaacggtgtt
     9541 ctcatgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt
     9601 gaaattgctc cttgtgactc agagttgaac ggcaggctca cgacctcttc cttaaacggt
     9661 gtttaaaagc ttgcatctgc gataataagc ccggccaaac gaaaaatatg aggagctatt
     9721 tttgtttggc tgggcttatt tattactgaa aataagtggt caatgcttgt gtcaaacttg
     9781 cagcaatctg ctgctgataa ctgtctgtta ctaattcctt taaatcacta tcattattca
     9841 tataacccat ctcgattaaa atagaaggtt gaagattttc tcgaagaact tgatagttcc
     9901 cagtggagat tcctcttgaa tccagaggca gatcagtgaa ggagtcagca attgtctgtg
     9961 ctaatgtttc ctcttgtcca tagtaataat atgtcgtgat tccagttgct tcattcgcat
    10021 attccgtaga gtctgcgtgt aaactgataa agacatctgc ctttttttct tcactgtagt
    10081 cgcatatatc tccaagctgt actaattcat ctgtagtatg ggtcaaataa accgttgctc
    10141 cagcatcctc tagcgccttt tttactttta tggctgtact taatgttata tctttttctt
    10201 ctgtttgttc atcattgctt aatgccccag tatcatttcc gccatgccca ggatctaata
    10261 caatcactgc atcagatagc tcttccggtt ttgtttgtat attctttgtg actttggttc
    10321 catcttcgag ggctaaatat ccagtatagc catcatttgt cttgatctga taagcatttt
    10381 ttgtttcaga aagaaactcc gccagctctc cgccgtttat agttgcaagt ggggtagtta
    10441 gcgagtcatc tgtatatagt gtacggtcat tttttccgat tttgattaat gtctcttgtt
    10501 ctgtatcatc aaatgattcc tttgtctttg taaccgtact gttcgtactg ctgaccgttt
    10561 gtccaactat ctttttttga ccaaaaacat ctatttgaaa acagataata atgataatga
    10621 ttgcaaatac aaaagctcca attatttttt tcattcttga ctctcacctc gtagatccag
    10681 actaagggcc aaaacttaaa atcagcttaa gtaagcaaaa aaggggctgt aaaagatata
    10741 accgtagaaa ataacattac tgctaaaatg atttttttca tttttggcat atcctgaaat
    10801 ctccttttga ctattcgtgg tgattcatct acccaataaa tagtttcctt cattctacaa
    10861 acaattaatt tcttcgttat taaattaaaa cacttcctgg aacaccgaca taataaataa
    10921 aaatataccc tgataatcat gagagaataa caaatttgta tttatgaaaa actacctata
    10981 taaagaagca agatataacg atcttcgtca tatcttgctt cttcacctaa ctattttctc
    11041 cctatataac agctttacgc ttctcctaat acaccattca aatataaatc gatcaattgc
    11101 gccacattta gcggttcttc cacaggtctt ttcgaccgag tgatgtaagg tgctaacact
    11161 aagaaaaaat gccttgctgc gatttctttt gaaatcccat ttcttagcac agattggttt
    11221 gctgaaaaga tttcttcaaa aggttcacga taatttttct tccatagaaa gaaaagatga
    11281 tctcttgttt cctttgaaag attgctttca agatcgtgaa gcatcagaaa caaatcgacc
    11341 agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg ttacttgaaa tcccgtttcg
    11401 cttgtttgaa cgatttctct caaagctttt ccaacatctt ttaagtggtt ttctaaaact
    11461 gccgtatata gtttttcttt atcaccgaaa taatgataga gattgggctg ggtgatccca
    11521 actttttgtg cgatttctct tgttgaagtg ccgagatatc ccttggacat aaacaagtca
    11581 gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt atcgatggac catttttcca
    11641 cctgcttttt ggttattctt tttctttcaa tacaccatct tccatcataa atattctatc
    11701 gcatttgtca atcagccgtg tatcgtgagt aaccatgatc gttgctttat ttttgtcttt
    11761 tgattctttt gccaagatat cgactacttc tcgtgccttt tctgaatcca aactagcagt
    11821 tggttcatct gctagaataa tggacggatc attgtacaat gcacgagcga ttgcgaccct
    11881 ttgccgttct ccaccagata gttcttctgg atacttgttg atcaacttat ccacgccaag
    11941 ctgctcaaat aattcgtttg cttctttttg acgatgttca tgtttaatct tatctactaa
    12001 taataactgc ttttttactg tcagaaaagg aaccagattg gaagcttgca aaataaagcc
    12061 aatttcttta aaacgtagtt taacgcgttc gctttctttt tgattactga attcttgatc
    12121 attgataaat acagagccac tgctaggcgt ctgcaatccg cctgccaaag tcaaaaaagt
    12181 acttttacct gaaccagaag gaccgatgat tgcaacaaat tcccctttat cgatactcat
    12241 ggttgtttct ttcaatgctt taactacttg gtcgccatct tggaaacttt tttctacttc
    12301 tttaaattct attgctttca tgttcttcct ccttcttatc caattgcctt caacggatcg
    12361 atttttacga ttgtccgaac agaaaatagt gcgcctaaca cagctactac taacatcaac
    12421 aagctaatga cgccaaaaaa cagccaattg ctttggaatg gaaccgcatc aggtagaaca
    12481 agggatgttc ccactgttcc taatagacca agaagaatac caacaaatga taaaatgaat
    12541 gtttgtgcca ctacagaacg cgcaataaat cctcctgtga tcccttgtgc tttcattaca
    12601 ccaaagatat taatcttttg catcgtcagt acataaatga aaatcccgat cacaatagcc
    12661 gcaataacga tcaaaaatcc gatcataaat ccaaaagtca atacttgcgc attatatcca
    12721 ggtaattcat tgatgaattt agaaattttg atctgttcta aatcttcagg taaattactg
    12781 attttacctc tgacaactat tgcgttaatc cgtgcatttt cacttgtatc ttctttttcg
    12841 aagcggattt cttggtaagc attgatggtt gtatagagaa ctggtgagac attgaatttg
    12901 gcatgatccg taaagccagt tagtttaaac gtcttgtcac taccagataa cttaactgta
    12961 tctcctatgg ctagaccata ttcttctttc aaactaatat cgcctactgc ctcatcatcg
    13021 ttatcaaaag cttttccttc aacaagattg ggcatgatga attgattttt atcgataccg
    13081 aagaagctaa cattgatttt tccttcttct gtactgtctt tactagtcac gactccaggt
    13141 gtttgggcaa gatacgccac tttcccacct tcgacttctt ctgctatctt cttagtaatc
    13201 atggacatcc ctagatttga atttgcatct ttcgaaagca caatcgaatc tgcctcccac
    13261 ttgtcgactg ctgttcgatt atcttgagct aagccataag ctaacccagt caaaaaaaat
    13321 accaggtagg cgatcaaaaa catcacccct gcaactaacg cataacgtaa ctttgaatgc
    13381 ataatttcat ttaatgctaa aaacatttaa ctcctcctcc tgaaataatc attttataca
    13441 acaattttat cacttgataa atttttggcg aatattatgc tcaaaaaatt cttccgctat
    13501 ttcattatat gaatttttta ttctcgattg attaatattt attatatccc cacacaataa
    13561 aagaccgtga tatttctcac agtcttttat ctactctatt gctatttgct ctcttagcta
    13621 gtactttaaa agctaactca ttgaggtagt gaaaacgaga ggtgtattca gacagaaatc
    13681 caaaatcctc aactgctagc tgtggaatcg tagttttgtg tttcacgata tccgcaagca
    13741 ggttcaaatc tgataataag aattcttggc ttatcagctg tccgccaatt atttctaatg
    13801 tatccggatg gtagatcagc tttgcattta ctgttttgtt ttcttcgtcg tattgtggaa
    13861 atagatcaaa ttctcctgaa cacgaaccgg tattttgttc caagaatggc gcttcatctt
    13921 ctgtcagccc tacagttcct aaaaagtagt ctataatgac tgctgctgtt atcttttgtc
    13981 tgaggtcgta agcggttggc tgaccactca aagttagcgc tactgctctt gccatatgta
    14041 cagctcgagt aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa ctgactggga
    14101 cgatatctcc aatcgcatag atgtccggat cacttgtctg cagatattca ttgactatca
    14161 cagtctcatc ctcatacaaa gccacttttt cttttaacaa tgtcgtatta ggacgagttt
    14221 gggcagaaaa aatgaccata tctgccttta ttgtttgaga cagaagttgt acggatacta
    14281 ctttctcttc tgattttttc caatcgatca agaattcatt tggataaaaa tttatttgct
    14341 tagtttgcag ctgattttga aaactgttga tcatatcctt atcgaaatat cttgataaaa
    14401 cattcggcat ataatcaact agatgaataa tgtatccttt atttctcaaa gcatctgcta
    14461 attccaatcc aatgtaacca gcaccgaaga tcactacttc ttttgcttct tttaaagcgg
    14521 caagtacttg cttagattgc actagatttt tataggtgta gacattttca gcttcaattt
    14581 gtttatacaa ggtgacagga gaggaacctg tcgctacgat taatttatca taggttatgg
    14641 tctcgttttt tccttctttg gatgcgagga tgagctgatg tttgtctgga tcaagttcag
    14701 ttactctcca ttcggtgatc agttttgccc cactttcttg aagagtccgc atgttatggg
    14761 caacttcagc taaactacgt atctttcctt ttaaataaag gttgattcca tttgaaacat
    14821 agcctagtcc atctttatga tcttcatcga tcagtaaaac ttctgctttc ggttgaagtt
    14881 ttcttagatt tagggcagct gtgatgcctt catgggaagc accaatgatc acgattttca
    14941 tgttttattc atccttaaat tcaatttttc taaataacat tcttttaaaa attggttcaa
    15001 ataggggaaa tcataattat attcgttgct agtgaataca aaaactttag cggacttctg
    15061 tgacgggtac tctttcaatt ggctagtcaa aatgatatca tactgctttt ctttctcgta
    15121 ttcgactaaa tcatatttga tcgtattcag caattgttcc tggatgattt ctttcacgat
    15181 atccgccatt agcctttcat aaggaagatc ataagcaatt tgcagacgta attctaactg
    15241 cttgatcagc gtccgtaaaa tagatgcata gcgagcaaat aattcaaagc gggcattttc
    15301 ctcgtctact tcagtaaaat caacaatagc aaatgtttgt ctgaccaatc tttcacataa
    15361 aaccaggaat tgtttatctt ctacttcagt taaccgctgt tttaatcctt cttggccaaa
    15421 aatattcatc catcctctaa aataggcaaa atgagcgtga ttttgcatca ctgtcaacgc
    15481 aatcttttgc gaaatctctt ctggaaaaac ataaaaagag aaatattctt ctatcgattg
    15541 tttaaacgtt cgattcaatt gagctactac gtcgtctttg tatttttcgc ttatcaaaaa
    15601 ctcctggaca tgcgagttat gtgtatctaa tgtaaagtta ctgatcatga aaagataaaa
    15661 aaccaacgtt tcattttgat tccaaggaaa ggcatattga aagaagtaac gggtcaaaaa
    15721 tgtatggagg tccaataaaa attgtgtcgt ttgaaacaat ccaacaggaa gattgctata
    15781 atctggtttg gattttttcg tacagcgcat tcgcttcttc ataatggaaa gccatatttc
    15841 aaatttcaat cgtccttctt ctgtaatagc aaaactaaac tctttttgca gcagatctaa
    15901 aaaagtgttt cctgataaat cactgtattc ttttttgttt tcaagatagg gacgtccaaa
    15961 ccaaaataat tggaaataaa aataactaat ttgactttct ggaccggtca gccgcccgct
    16021 ttttattttc agatgaaact catttaaaga atcatttaat tccttcactc tgcgagacaa
    16081 agtagccgaa ctgatattaa gttccatgac aagtttctgc cgatcaaatg agccattttc
    16141 caacagataa agaagtatct gatattttgt agaatagcgc aagtaacaat gataataaac
    16201 atcgactgct gctatatttc catgtctgat taataaaata ttcccttctt ggataacaat
    16261 ttccaattgt ttgatatttt ccaagttagc ttgtaaataa aacagatctt ctttcaaggt
    16321 agcccgatca actgataagc gggtaagtac gttattaacg gaaacctctc ctttttcctc
    16381 taataaaagt tggaaaagtt tgatttgccg ttgttcattt ttttcaagca aactgaaaag
    16441 ttccaagtcc actccttcaa gcatttctga ttttaacagc agaagtttcc ttctcatcct
    16501 tgaaaaagag gatctgtgac agttatcaca gatccataca aattctaatc acttagctgg
    16561 ttggcccagc tgtcagttcc catgtgacca tgccttgata cgttgcgcca ggttgagcat
    16621 tcgtactgct atttacctct aagagtaaac catcggtatc gggaaagtca acgagaacct
    16681 ctcctttagc tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc tgaatcaatt
    16741 gctttgatcc agaatgattg aagtataatt ttgttccgct taattcttga ccttgatcat
    16801 tcttaaataa agaagtcaat gtacctgtca gttgccaagg agacgtgctt ggaagccgag
    16861 tatcccgcac atcaaaagaa aaatcgcctt ttgattcata ataacttgtc ttcaacggtt
    16921 tggggagatc gttaccaaat gtaaaatcat ctggtacatt aactaatgtt aattcttgtt
    16981 tggggacaag aattgtttta gcagatgttt ggacaactgg gttaggaagc ttagcaccat
    17041 ctgctaatgt aatattgctg gaaagtacag ctgcctttgt cagtatgctg acggattgta
    17101 aagaagtatc tacagtattt aattgatagt tgtaagtaag atgaatggta tctcctattt
    17161 tgctcaatag atctgctggg acagaaactc cttgttgaat gccggtatct gcgatagttg
    17221 taccatttaa ttttaaggaa ccattgatat aagtagtatt ttcaggaagt gagatcgtga
    17281 tttttttgtc ggttatcgca tccccagatt ttgttaacgt gaatgtcaca ttttcttgaa
    17341 tcgtatctcc tgatagagca gacaggggat ctcctgtctc aggttgtctg ctaaggtttt
    17401 ttagctgact atctgcactc acagatggaa aattagccac tgttatttga ggaagagtgg
    17461 tgattccagc ttgttcagac tgccatttgt acgcaaaaga cggatacatg ttagtaagta
    17521 catcgtccac caagcgataa tcaaaataat attgccctga agccaatgca ggtagtgtag
    17581 aagtagctgt atttacaccc ccgttatttg attggatcgt ttggaaaagt tgatctgtcc
    17641 atcctgtcgc taatttttgg gttcctttcg aataaagcct gtaatggatc gaaccctttt
    17701 ttagttcagt tgctgaccaa ttggccgtga cttggtacga tgcagaagaa aggagctttc
    17761 cccactgact atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc ttgtactgct
    17821 ccaatacctt taaagcaaca ggagaaagca atccgccttg cgcatctttc ccactgtaca
    17881 cgatattggc tgtctgattc aatccacttg tgcttgctgg agtatgagtc agtattgctc
    17941 cttgtgtaac gttttttcct gtaaaatagt tgactacgcg atcaaagcga ctaaatggca
    18001 actgatcaaa acgtccttca gtcagcccca tcgtcaatgt ccgactattg gtagagcctg
    18061 gaaggttctg gttatttcca tccacgtatt ctgtggtatc agcaaatgta ttatattttc
    18121 ctgttgaatc acttggtgag atgtaactat tccacgtatt tatctctgca cttgcagaaa
    18181 atgcatatgc tcctggattc aagtttccgc cagttaattt aggataaaat ctacctgcag
    18241 ctggagctaa aatcgaattg aacatacgat cttggataat attattcgaa tcataaaaat
    18301 caacagaaaa aacggccttt ttagacatag aaggttgtaa tttacccttg gtcaaaattt
    18361 tgtttggact cgtatcatat ttatctccgt tttctgtcat cttcgcgaca ttagctgtaa
    18421 catcaaattg aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata atagcaataa
    18481 tatctgtcaa tagagtattt gtatcccacg accccatccc aaaacgcaag gtatggcttc
    18541 ctgctgataa taaacttccg ctagtaacct tgatgggggc accggcaata tacaagtagg
    18601 cgttgttcca gtcaattgct tttaatacat cactgcttga tacatgttcc ggcaaattga
    18661 tgatcgccac aaatgcaaat ccttgtgcag tgatattcac caaatcaagt acatttgtta
    18721 gtttcacatg gctttcccaa tgaaaacgga ccgtgccacc tttccaagga tcatttgaac
    18781 catcgttcaa ataagtagca gtcaatttag gcaattggtt agccccagtt agttggacaa
    18841 gcttcccgcc attgactgca ctacgtccac catttggagg tacgatattt tcatacgtgt
    18901 atggatcata agaaatggca ggaggcgttg ctccaacagc ttctgccgaa ggtgaaaaga
    18961 taaatatact taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat gaaaatttat
    19021 tttgtagcaa catgttcatc ctccttttcc tttcttctcc ataacaacaa ccacgcaaga
    19081 agccataatt ctataccaat gatcaaataa agaaaacgga tcttttgttc attcgtctcc
    19141 gggaatacgc catgttgatt agattcttta taaatcggta gctgtgtctg ctgttttgat
    19201 gcgccattga ctgaaccact tggatgcgga attggactta gatcataaaa tgtaatcgta
    19261 cctgaacttt tggtatctgc ttggactggc tcttgccagc tgaagaacag caaacttgca
    19321 ataatccctc ccaataagat cgatgttttc aacgaaatca cctcatcatg ttgtttctcc
    19381 ttttttcttt ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca ctagaactgc
    19441 agcaaccaat cctgcataaa gccatgggaa agattgttct tgtactgcaa aaacggaatt
    19501 ttcatttact ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa atcgccattc
    19561 attactgtcg cctgattttg ctatgatttc tgcgcggtaa tcgccggctt ttgctttatc
    19621 accagctaaa tcaagattca agtgaaaatt cgaatttggt gcaactaaaa atgcattaga
    19681 cgtattttca atataaagct gattttcata aaagatcttg ctcgtaaata tcaaattgct
    19741 gataatccta ggctgaggat tttggaaacg aagctgaaca gttggctttg cgttgacctc
    19801 gtccaaatct cctgataaaa gtttcagatc cggttctatc gtctcttgac tttcttgtaa
    19861 aacgactgcg agattataag caatacgatt tttgatttgt gctccttctg tagcattttc
    19921 tgtttcagct tcttctttag gtgaaacatg aatacccgct aatacacgtc ccttaaagct
    19981 ttttgctggg attttcactt ctataggtac agttgtttct gcatgtgctg atacattgat
    20041 ttcctttttc gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct catctaacgt
    20101 tgggtttttt tgactataat cgatcgttcc accatcactc gttaccgccg gattgacaga
    20161 gacctcaaat gttcgttctt catctgaaga attgctgata attacttcta gtgtctgatt
    20221 ttgttccggt gccactaata aatcaaaata accgattgct ttatctattt ggttctctgg
    20281 aaataccggt ttgattccaa agtctcctgc tgaatccttt gcttggctga ttgacggaaa
    20341 aatgaaaaat agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac tttgattcat
    20401 tgtccaccac atcctttcaa caacaactgc ggtaaaaaat ccttcaccta tagtattttc
    20461 accgcagttt gttgaattta tttcatactc tatgctactg ggccagccac gaggttccaa
    20521 gtcaaatcag ccgtgaatgt tccagctgct gctttcggca ctttcagtgt gatatttgct
    20581 ggatcataaa cagaaagcca tgttcccaga ccttgatctt ttgctgctgt aaatattggt
    20641 gtcgctgcac ttgctttact caacccagtt acagtttgac tagttggtcc agtggattcg
    20701 ttatctgacg tacttttaac agtggatgtt ccaaaatcta attcagcgcc ttgcaaaact
    20761 tgtgaaccgt ttacaaaatc tgaaaccgaa actgtcacgt tccacccttg tgcttggcca
    20821 tctgcaccta cacctcgacg atctgatact tggagataag gtgtatcgtt ttttgaagcg
    20881 tctacttgat acgtttttgt tccgctttca atctcgtgtg taccaaatgg caattcggga
    20941 actacatcca gtgttaaagg acctgtctgg ccagttgctg gattttccgg atctgctggc
    21001 tctactggtt ttgaaggatc agtagggtta agaggctcca cggtatttgt tggttcagta
    21061 aaggtaatat ctccttttga agtcgcttga ccctcagcaa aagctggtgt tgccaagccg
    21121 cctagaagaa gtgttgaaag taataatgtc ccaatcatgt tctttttcat aataaatcac
    21181 tcctttttct tagtgaccta agtatagtag agacagtatg caaaaaatcc tcatgaattc
    21241 atccgcttac ttgaaattat gacccttttt gtataaaaga gaaaaaaaca ataaaatcgt
    21301 ttacatgaat attcttcctt ttttattaa
//
LOCUS       DAZKMB010000043        20944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000043 DAZKMB010000000
VERSION     DAZKMB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.043"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACG8V3_001393"
     CDS             complement(<1..563)
                     /locus_tag="ACG8V3_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0299940.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACG8V3_001394"
     CDS             complement(750..1400)
                     /locus_tag="ACG8V3_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299941.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8V3_001395"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8V3_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0299942.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8V3_001396"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8V3_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0299943.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8V3_001397"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8V3_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0299944.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACG8V3_001398"
     CDS             complement(4926..5657)
                     /locus_tag="ACG8V3_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0299945.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACG8V3_001399"
     CDS             complement(5661..6926)
                     /locus_tag="ACG8V3_001399"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0299946.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8V3_001400"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8V3_001400"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0299947.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8V3_001401"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8V3_001401"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0299948.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACG8V3_001402"
     CDS             12268..13770
                     /locus_tag="ACG8V3_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0299949.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACG8V3_001403"
     CDS             13772..14509
                     /locus_tag="ACG8V3_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0299950.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="ACG8V3_001404"
     CDS             complement(14680..15873)
                     /locus_tag="ACG8V3_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0299951.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="ACG8V3_001405"
     CDS             complement(15952..16158)
                     /locus_tag="ACG8V3_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0299952.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="ACG8V3_001406"
     CDS             complement(16151..17293)
                     /locus_tag="ACG8V3_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0299953.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="ACG8V3_001407"
     CDS             17805..18440
                     /locus_tag="ACG8V3_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0299954.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="ACG8V3_001408"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="ACG8V3_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18985..19032
                     /locus_tag="ACG8V3_001409"
                     /pseudo
     CDS             18985..19032
                     /locus_tag="ACG8V3_001409"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19048..>20944
                     /gene="tet(M)"
                     /locus_tag="ACG8V3_001410"
     CDS             19048..>20944
                     /gene="tet(M)"
                     /locus_tag="ACG8V3_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0299955.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVR"
BASE COUNT         6161 a         4297 c         3720 g         6766 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgat
//
LOCUS       DAZKMB010000044        20760 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000044 DAZKMB010000000
VERSION     DAZKMB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.044"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(135..1022)
                     /locus_tag="ACG8V3_001411"
     CDS             complement(135..1022)
                     /locus_tag="ACG8V3_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0299956.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            1207..2046
                     /locus_tag="ACG8V3_001412"
     CDS             1207..2046
                     /locus_tag="ACG8V3_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0299957.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2242..3216
                     /gene="sgrA"
                     /locus_tag="ACG8V3_001413"
     CDS             2242..3216
                     /gene="sgrA"
                     /locus_tag="ACG8V3_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0299958.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            3514..3870
                     /locus_tag="ACG8V3_001414"
     CDS             3514..3870
                     /locus_tag="ACG8V3_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299959.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            3915..5030
                     /locus_tag="ACG8V3_001415"
     CDS             3915..5030
                     /locus_tag="ACG8V3_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299960.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5056..5496)
                     /locus_tag="ACG8V3_001416"
     CDS             complement(5056..5496)
                     /locus_tag="ACG8V3_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0299961.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5656..6162
                     /locus_tag="ACG8V3_001417"
     CDS             5656..6162
                     /locus_tag="ACG8V3_001417"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0299962.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            6330..8243
                     /locus_tag="ACG8V3_001418"
     CDS             6330..8243
                     /locus_tag="ACG8V3_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0299963.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            8248..9249
                     /locus_tag="ACG8V3_001419"
     CDS             8248..9249
                     /locus_tag="ACG8V3_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0299964.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(9451..9657)
                     /locus_tag="ACG8V3_001420"
     CDS             complement(9451..9657)
                     /locus_tag="ACG8V3_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0299965.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(10303..11151)
                     /locus_tag="ACG8V3_001421"
     CDS             complement(10303..11151)
                     /locus_tag="ACG8V3_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0299966.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            11315..12217
                     /gene="gnd"
                     /locus_tag="ACG8V3_001422"
     CDS             11315..12217
                     /gene="gnd"
                     /locus_tag="ACG8V3_001422"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0299967.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            12219..13220
                     /locus_tag="ACG8V3_001423"
     CDS             12219..13220
                     /locus_tag="ACG8V3_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0299968.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            13217..13501
                     /locus_tag="ACG8V3_001424"
     CDS             13217..13501
                     /locus_tag="ACG8V3_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0299969.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            13534..14331
                     /locus_tag="ACG8V3_001425"
     CDS             13534..14331
                     /locus_tag="ACG8V3_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0299970.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            14345..15178
                     /locus_tag="ACG8V3_001426"
     CDS             14345..15178
                     /locus_tag="ACG8V3_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0299971.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            15196..16053
                     /locus_tag="ACG8V3_001427"
     CDS             15196..16053
                     /locus_tag="ACG8V3_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0299972.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            16075..16584
                     /locus_tag="ACG8V3_001428"
     CDS             16075..16584
                     /locus_tag="ACG8V3_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0299973.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            16621..17022
                     /locus_tag="ACG8V3_001429"
     CDS             16621..17022
                     /locus_tag="ACG8V3_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299974.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            17134..17487
                     /locus_tag="ACG8V3_001430"
     CDS             17134..17487
                     /locus_tag="ACG8V3_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299975.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            17521..17964
                     /locus_tag="ACG8V3_001431"
     CDS             17521..17964
                     /locus_tag="ACG8V3_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0299976.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            17981..19066
                     /gene="uxuA"
                     /locus_tag="ACG8V3_001432"
     CDS             17981..19066
                     /gene="uxuA"
                     /locus_tag="ACG8V3_001432"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0299977.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            19059..19772
                     /locus_tag="ACG8V3_001433"
     CDS             19059..19772
                     /locus_tag="ACG8V3_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0299978.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(19979..20128)
                     /locus_tag="ACG8V3_001434"
     CDS             complement(19979..20128)
                     /locus_tag="ACG8V3_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299979.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            20524..20724
                     /locus_tag="ACG8V3_001435"
     CDS             20524..20724
                     /locus_tag="ACG8V3_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0299980.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7100 a         3204 c         4316 g         6140 t
ORIGIN      
        1 agtgatttgt tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt
       61 gcctcaaaaa taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat
      121 tgggtcaaat ttgtttattt gaatatattt tttacataag gatctttaat agttacattg
      181 tcttttttca tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct
      241 tcggtagcaa ttttttctag ctcagatttg tatttgtctt tattagaacc tttatcctgt
      301 ttttttacca tttttactag ataatagctt tgctgataag tcgttggatc tgttacagga
      361 atgatatcag aaacttcccc gttttttagt ttaaatgctg ctttttttac ttcagtagga
      421 atctctggat tggttgaatc aaaattcatt tttccatcag tttcctttaa tgttttattt
      481 tttccataag cttgaacaag cttggagaaa ttttctcctt catttgcttc tttttttgct
      541 ttatctgctt cttctttagt cgaaaataga atgatctgtg tactaacttc tggatagaat
      601 tctttccaag cagtgtctag atctttatct gttaatttta cattggcttt caaaccgtat
      661 tggaaagcca gcatctgttt aaagagtttc ttatatgtct cttctgtttg gccaactgct
      721 tttaattgtt ccttgtagtt tggaccgagt tttttgattt gttcattata ttcttttgtt
      781 acttcctttg aagaaatctc ttttccgtat acatcatcaa atgctttgaa gacaatcatt
      841 ttttttaata gtgtttgatt atttgattta acttttgggt tatcataaaa atcctgaact
      901 gtaagtgttc cacctttaaa cgttgcaatc gtttcattgg acttgctaca acctgagaat
      961 agtaaaattg cacatgctga aaggcacata attagtagtt gttttttcaa aaaatttccc
     1021 atcctttcct tattaaaaac agtctaacag aagaattagg gagacagtct ttcattttcg
     1081 taaggttgct gtagatattc ttagttcgta aaaatctatt ctctctatga tggagaacat
     1141 ttacatagtt ttcacgagac aagaaagaaa agggttgtta aacttaatat aaaggaggga
     1201 tgaaagatga tcaggattat tcggttgatt ggatctgttt taatagtctt agttggtttt
     1261 gttttaggaa ttcttgtcaa taatgcactt gcggatatgt ttattggaaa tagtgaatgg
     1321 attggagcaa ttgccggtac ggtcggacat ttagctgttt ttttactagc tttatttctt
     1381 gctagtaagg tcgaaggaaa aagaatagaa gattatggtg tttctgttcg aggaaaagac
     1441 tgggggtatt tagcgggagg tctcgttgtt ggaattggag tgtttctgct tattactagt
     1501 ccattatatt tgacgggggc gtatcggtta gattcaggaa acgcaaacat tgtgccgctg
     1561 ataacgagtt ttattttatt tattgcagtg ggggcatcag aagagctttt attccgtggc
     1621 ttttttcagc atcagatgct tagatttggt ccgttaatag caatgattgg atctgctgca
     1681 ttatttgctc ttttgcacgg attgaatcct aacatgacat ttctggcagt tttcaatatc
     1741 tttttagcag gttgcttttt cagcgcactt atctacagta caggaagttt atttacggct
     1801 attggtgctc atattacttg gaattggaca caaggagcta ttttgggtat accagtcagc
     1861 ggaacgcaag aacaaggttt tttctccact ctgatccaat cgcaaaatac aataattact
     1921 gggggaaact ttggggctga agcatctata tcaactacat tattcttgtt agtcttgatt
     1981 gttggtttgt tactctatgc atataaaaca aagcggatga agaaatacac tgctgtttat
     2041 caataaaaga tgaagaaagc aaggctgata tcggtcttgc ttttttaagt ctgtcattga
     2101 ttaaacgaaa aagtaaacaa attcaagcga atacacgaca tcttttgtta gattttcaca
     2161 actgactgtc gtgttctcat cattttcatt ttatataatt cctttatcaa atagaaggta
     2221 attatatgga gagtgataga aatgaagaaa acagcgaccg tattatttgt tattcttggg
     2281 ctgagtataa ttgcagcaat gattggaatc agtacttctg caaatgaacg ggcaaatgag
     2341 aatctttcat ttactgtgaa aactgatcgg attgtttatg acatgacaca acaagtcatt
     2401 actattccag tcaaaccaaa caaaagcgtg aatgctagtg atgtacatgc tgttctaaca
     2461 tatggttggg atggtaatgg aagttcagaa aaagttattg gggaagttta tttaaaagac
     2521 gtacaatgga ccgctggtat tgagtataca ataatgattt ctgcagaact atcgattgat
     2581 gagatcaaga gtaaagataa agttgatttg attgtttttt atgatggaca gatgacgatc
     2641 acggagaatt tgaaaccaag cagttggaca gttgttggac cgccaagcag tacggatagc
     2701 acaagtagtg aaagcagtac agaaaacacg agcggtgaaa gcagtacaga gagtacgagc
     2761 agtgaaagta gcacagaaaa cacgagcagc gaaagcagta cggagagtac gagtagtgaa
     2821 agtagcacag gaagcacgag cagcgaaagc agtacggaga gtacgagtag tgaaagtagc
     2881 acaggaagca tgagtagtga gagtagcaca ggaagcacga gcagcgaaag cagcacagga
     2941 aaagtaaata atgaacttga tttggtacct atcataccaa ctaaggaaca aaagagaaat
     3001 gcagggagca acctaaaaag tgtgctaaaa cagcctgatt ttgtttctcg tggaattgat
     3061 catcaagaaa aagcgaaaga aacagataat agaaaaagcg atttaccaga aactggtagt
     3121 agaaccttga acaagctaat tacatggatg ggtgtgttac tcattttgat tgtaggagca
     3181 agctattttc gttctctctt ccatcgagtg aaataaagaa taaaagcaac agatctaaaa
     3241 ttgaataaaa cgatagattt ttgagtgaat atttcattcg agagtgctgt attgcctggc
     3301 gaagttgtag ctttgccaga tctatttttg tttactaaca tatttgagaa acaggaaata
     3361 acaggaacac aaatggaaaa taagagcaag aataattgac aatgcacact gcgcatggta
     3421 gtatctctga aaaatcagta acgaatctat ttgtacgtta ttcatgtagc tgtcggaata
     3481 agtctaaata atagataaaa aggagtatgt actttgtttg aagaatttat tgatatcaat
     3541 gaaaggcaag tatatcaatt tttgaactat tgttatgaaa gagacgagaa attatatgtg
     3601 gtgaaggata tcgctttaga tctaaactac accctggcta agatgaattc ggtgattcaa
     3661 caagcagaat cgttttgcga aaggtatcca gaatataaat tatcttttct ttcagaaaac
     3721 aagatgataa aagtcgagtt ttcttctcag tttttactga gtaaagttta ttctatactt
     3781 ttagagggaa cgattggtta tattttactg gattcacttt acaaaggtac ttatcaatcc
     3841 ttagaaaatc ttagtcaaaa aattatataa gtttgcgtac gattcaaaga aaattaagag
     3901 aacttaatac tattttgaaa aactatcatt tatcttgcag cttgaaaaaa ggtaatccga
     3961 tagaaggaga agaataccgt atccgatact tttttcactt gatgtattgg cagtcattcg
     4021 acgaaaatca ttctgatata ttatcaagta taaaaaataa acgtcaacta ttagaaaaat
     4081 ttgcgaaaca tgcgccatat acgcgtagta tagatagaaa gaaatggatc agcctactaa
     4141 gaatttcgtt gcaacgaatg aaacaaggcc actcgattac gtatgtccca gaagagatga
     4201 acaaatttgt gcatccattg ataacaaaag aagagtttac gcattacata ctagagcctt
     4261 tttttgaagc aaacttctgg tcgatgaatc acatcactcc aacagagcaa gcttaccttt
     4321 attttatgtt tggtgtaatg aacacctatt tggaagaaga tttgttggat actccagata
     4381 aagtacaaga aagttggggc tggattgaat cgtctattgt agaaaatttt gaaaaaaact
     4441 tcaagatttg ttttacaact tcagaaaaaa attatctata tgtcaatttg gcaatgatcc
     4501 atttgtattc aagggttttt gggacaaaaa agaaggtgga tgcttttgga aaatcagcag
     4561 aagacagaga ttttcagcat atatttccag tgatgtatcc aatcatgcaa cagtttttta
     4621 gtggggtagc tcgcagaatt ccagagctta atacttatta tgaaaagaac agacggttga
     4681 tttttcaata ctgtatgtta gtcagggata tttttataaa acacgaacaa cctgtcatat
     4741 tctatattca atcaaagtat ggtaaaacac aggaattatg ggcaaagaaa aggatattat
     4801 ctatgactga acgcccaata aaatatagtg catccgctga tcagttacct gatattgtca
     4861 ttagcgatta tcctatcgac aaaaaggcat atgatcaaac gaatacacag atattttact
     4921 ggaatggaca acctacaaaa aatgattggg cgcgtctgaa agctgaaatc ataaaaatca
     4981 ggaatgagaa aaatagatcc atattggaaa aattggattt cgttaattaa gaaaagcaga
     5041 ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat cgacaagatg
     5101 accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa ggaacatcct
     5161 gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga taatcggttt
     5221 tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac tgagttcatc
     5281 tattctgtga acaagatttt tataactaat agaatgagta gagagcattt cggtcacgcc
     5341 gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt gacgaattaa
     5401 gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc gtcttgaaga
     5461 cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata gctaagtatt
     5521 tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt aaaataagaa
     5581 aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc aagaaaaaag
     5641 aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga tttacaaaaa
     5701 ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc attagtgaat
     5761 aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca acacgaagat
     5821 gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc tattcctgag
     5881 cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa tttgagtgtt
     5941 ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc agagtattgt
     6001 atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat gtgtagagga
     6061 gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc tttttacgag
     6121 aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc ttcgtttaaa
     6181 aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg agaaaataat
     6241 cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt ttgcgtttac
     6301 atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa caagtattaa
     6361 agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg acagtatatg
     6421 gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa actactgatg
     6481 ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa acaacggttg
     6541 agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac caacaaacag
     6601 aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa gtaagtgaca
     6661 aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat ggtaaagaat
     6721 atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca gaagacatca
     6781 agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat ggtgtagata
     6841 aagagattat caaacatctg attgctactt atctattgta cgatatcgct tttgcaaaaa
     6901 acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct acagaaaaca
     6961 caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa caagatggca
     7021 aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca gtcatgatcc
     7081 atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac aatgattttt
     7141 ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat agcggaggcg
     7201 attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata aatcaagaag
     7261 ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga gcagctacga
     7321 tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt gaaaattgcg
     7381 gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta gcccccgcac
     7441 taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat gaagatttat
     7501 ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat acaaaagctg
     7561 ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat ggaacagcag
     7621 atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga gggtataaag
     7681 atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac catgctgcct
     7741 acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata aacgtaaagt
     7801 atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag ctttacggag
     7861 cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa ttagcaaatg
     7921 ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt ttctattaca
     7981 aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca gacaataagg
     8041 gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt cagatgacag
     8101 tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact ggagaaaaga
     8161 aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt atcacatgga
     8221 tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt gattgcagta
     8281 ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat cacaccaaag
     8341 ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca agtatatgcc
     8401 cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga aatatcctat
     8461 ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga aaagaaaaaa
     8521 ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa aaatgattgt
     8581 cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa tatagattat
     8641 gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga agccattcaa
     8701 gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat tggtggaaat
     8761 tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga agaaattaaa
     8821 aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt tatttctttg
     8881 tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc tagctttctt
     8941 tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc agagaaggtc
     9001 aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt cttaacggat
     9061 ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt tgagaaagcg
     9121 ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa ccatgaatat
     9181 cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt aatgtttttc
     9241 aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct ccaagtcaca
     9301 aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact tgtgactgag
     9361 gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac acgattattg
     9421 tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc gtttaaaaaa
     9481 agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg gtaagcaaaa
     9541 gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac aaagatgaat
     9601 agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag ctttcataag
     9661 cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa atgtgacaag
     9721 tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat ggctaatgat
     9781 aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac gtctagacca
     9841 ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa agaatttccc
     9901 caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc aactgtgaaa
     9961 taaaattaca aatgataacc taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt
    10021 aattcaccaa aatttttttt aattttttag aaaataagaa acaaaaataa taaaaaaaag
    10081 aaaagataaa ttttttacta ctgtatacta actttgttat cgcatctaac aagtaactcg
    10141 aataatacca atacaacctt ctccaaagat gcgatgacat acgttctaac ccaccgacta
    10201 ttgatgttct gtaccaccct tgcagaacca aaaaagccac taccaaatat ttggtagtgg
    10261 ctttttttgt tttatggatt tttcgctttt tctgttatgg tgctattctt tgatttcaga
    10321 aatagtatgt cgaactttag cgatagaacg acgtgattct ttttcgttgt aaaacatcaa
    10381 aatcacatat agagagtcca aaatgcttag ttgagaaata cgggaagcta aagcttcaga
    10441 tcggaattct gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga
    10501 agaattagct tggctagtaa taacgattgt ttttgctcca gtttttctta cggtttcagc
    10561 aatatggatt gtttcttttg attgccctgt gtgagaaatc aaaacagcac agtctttttc
    10621 cgtcaataaa gaagcttcca tcaattgaat atggtaatcc gtgctgtgag aagttgcgat
    10681 aggggaacgc aaaaatttat gataagcgtc agttgctaca atctctgaac cgccaacacc
    10741 gaaaaaatag acacgtttag attcattgat aatagtagcc gcttttttta gatccttttg
    10801 ttgtaacaac ttttttgtgt catttaaagt tgaaatacta gactcgaaca ccttttgcgc
    10861 catagtgagc tcagtatctg ttttctgaat attttcgtgg atagaaatag cagatagatc
    10921 attttcttgg atcaacatgg ccattttaaa atctttaaat ccattataac caagtttttt
    10981 tgtaaattga aaaaaagtag agtcagcaat gcccagttca cttgctaaat tgctaatagg
    11041 actatgactt actttgcttg gattttctaa aatataatcg gcgatagcct gttcttttgg
    11101 gctgaagtcg ttgtacattg ttttgatttg tagtcttatg gagacgctca tgagaattcc
    11161 tcattttcat aataagatta tcattttttc cgttctcatt aatgttaaag atacaaggag
    11221 agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa aaattttttt
    11281 gtataatgag ggtataagaa atggaggcgc tatcatggaa attggtgtaa ttggtttggg
    11341 aaaaatggga ttgaatttag ctttaaactt aaaagagcac catcacaaag tacaaggact
    11401 cgacatttca agtacagctg ttagtcaggc aagagaaaat gacattcctg cttatcaaga
    11461 gatctctgaa tttctagcta cttttgagca gaaaaaaatc atctggttaa tgcttcctgc
    11521 tggagagata accgaaaaca tgttaagaaa actttttcca aaactcggac caggagacat
    11581 tattattgaa ggcggaaact cttattataa agattcaatc cgtcgagcag aagagtttca
    11641 gaaaaatgaa atcggttact tggattgtgg gacttctgga ggaatcgaag gagcacgaca
    11701 tcaagcctgc ttaatgattg gaggagataa agaaactttt ctatccgtgg aacaattatt
    11761 caaagatgta gcaattgaaa gaggatatct gtatgctggt gcatccggaa gcggacactt
    11821 cttgaaaatg atccataatg gaatcgaata tggaatgatg caagcaatcg gtgaaggatt
    11881 ccaacttgta caaaaaagtg agtatgcttt tgacttggaa caagtcgcac gtgtttggaa
    11941 tcatggttcg gtgattcgcg gctggctgat ggaaatcatg gaagatcagt taaacgaaca
    12001 tcccaattta agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt
    12061 tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt gcaactagtt tgttcatgag
    12121 gaatctttca caggaagaag atagtttttc agcaaaagta gtttcagcac taagaaatgg
    12181 gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat gccagattat catatagcaa
    12241 tcgttaattc cagcagtttc ggaagagtct ttccacacca cttggaaaga ttaaagtcag
    12301 ttggagacgt tcatttcttt actgttgatc aagagattcc aggaaaagaa ctagcggagt
    12361 tacttaaagg attcaatatc attattgcaa gtgttacgcc attttttcga cgagattttt
    12421 ttgaaaacaa agatgaactg ctattgattt cccgacatgg tattggttac aacaatattg
    12481 atttaaaagc agcaaaagaa caccagacaa tcgtatctat catacctgct ttagttgaac
    12541 gaaatgctgt agcagaaaat aatataacta atttgttgtc catgcttcga cgcacgacac
    12601 aagcacaagc aagagtcaag gacgatcgat gggaagatcg ggcggagttc gtcggtagga
    12661 cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg
    12721 aaattttgcg taacggtttt cgctgtgatg ttctagctta tgatccttac aaatcagatt
    12781 tatacatcga aagctttggt gcaaaaaaag tagaacttca agaattactg gaacaatcag
    12841 atatcatttg tttatgtgcg aatttgacag aagagaacta tcacatgatt tctgataaag
    12901 aagtcgatca gatgaaagaa aatgtttatc tatctaatag tgcaagaggc gctctagttg
    12961 atgaagaagc gatgattaat ggtctgaaaa gcggaaagat cgcaggatat gccacagatg
    13021 ttttagaaga agaaccggga cgtcaagatc atccgtattt agcgtttgaa caagtagtga
    13081 ttacgccaca tatatcggcc tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg
    13141 tcagagatgt cgaacagatc agtcagcacc ttcttcctga acgttctgtc cagacaaaga
    13201 gcaactatat aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc
    13261 tcaattttat ttatatgaag aaggtggtat ttcaaaagtt tctgatctct tgaaatcata
    13321 cggcgcgaaa agagtactgg tcacccacgg aacggtatca tgggaaaagg cgctacctaa
    13381 attagtattt ttgaatgacg aaacgattca atttttttat catcgttatt ctggagaatg
    13441 tagttatgct gaagccagac gaatagctac aatcatcaaa aaaatgaaat cgatttcgta
    13501 gttggcgttg gcggaggaaa actgaccgat ctagtgggtt atagtgccaa tcttgcaaga
    13561 atcgatttcg gagtcatccc tactttagct agtaattgtg ctccatggac acctcttgct
    13621 gtcatgtata aggagaatgg acaagcagaa gggaaaacag aacactttta cagacaagct
    13681 gcttttttta taacagatcc ccaattagtg attgattccc ctgttccata tttcattgcc
    13741 gggttagcag atacactagc aaaatggtat gaatctgagc tcatcttaga acaagatttt
    13801 ttgcaagatg aatcattttt gaagctagca caagatactg cgaagatctg caaagaagag
    13861 attttgaatc attctgctaa agcaatccaa gatatggcac aaagaaaact gacatcggag
    13921 ttcaaacagc tttcagagat tatctttgct gttgctggtt tagttggagg attaggagat
    13981 aaatacgcaa gaaatgtggc agcacatgct atgcatgacg ctatcagtaa atatattccg
    14041 gaaagccatc gttttctaca tggagaaaaa gtagcgtatg ggattttcta tcaactggca
    14101 ctagaaggtc gctgggaaaa aatagacgag ttatatcctt tgtatcggga attagaactg
    14161 ccggtttctc tagaacaaat gggattattt ccgaaagata aacagataat agataaaatg
    14221 gctgaattta ttgattcgaa agagaaagtg catttgattc ctatagaggt aacacagaaa
    14281 tcactgatac agtcaatgat ggaactggaa ggttatatca gaaagatctg aaggaggagg
    14341 acacatggaa acattaagtg tagtacaaag tttgttgatt gctttatggg tagccgcggt
    14401 tatgtcccgc tggttaggtg gaggagccac gttgacctta cgtttttctc ctttgatgac
    14461 tggattagtc gtcgggttga tcatggggga agttgcacaa gcgatgattg ttacagccgc
    14521 tttgcaaatg atttatatgg gggttttttc tccaggagga tcaatgccag ctgaaccatc
    14581 tattgcagca gctatcgcag taccagtcgc attattaggg gatctgtcgc cagaagcagc
    14641 gattgccgtt gcggtgccag tcggattgct cggaagttac ttgtatcagt ttcgtttttt
    14701 catcaatact tttttaggta aatatacaga caaagctgta gaagatttga atacaaaagc
    14761 tatggcccga tcaattattc tttatccaac ggttgcctca tttttattat tcgttccgct
    14821 tgttttcatt gctttatatt taggggcgcc attgatcgct gatgtaatca aagctttaga
    14881 gggaacagtc gttatccaca ttcttgaagt agtaggtgga gggttagcgg caatcggtat
    14941 cgcgacaact gtatacgtta tcggtcgaaa agattatctg gtctttttct tccttgctta
    15001 ttttatgagt gttgttttga aatctttaga tattacgatg gttacttatg ccatttttgg
    15061 gattattatc gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac
    15121 aagcaataac aattcagcat cttttgatga cgaagatgac tatgatgacg gattttaatt
    15181 taggaggaat gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc
    15241 acgaaaaaag atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac
    15301 tcttttgaac gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa
    15361 ctttataaag atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac
    15421 actcaattaa gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa
    15481 agagcagaag aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac
    15541 acgaaagcgg gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc
    15601 gtccaatata tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc
    15661 gcactttttc cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca
    15721 agacaagctt tcactatggg ccgtaatgct acaggcctaa agcaaaacac tggtgttcag
    15781 acagcgattg aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac
    15841 tatgtaaaag tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag
    15901 gaaattcttg atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac
    15961 ttgttctata cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata
    16021 ttaattgtgc tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt
    16081 gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta
    16141 tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa
    16201 tttatgagag atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt
    16261 acaccagaga aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt
    16321 ttgatcgcaa aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa
    16381 gaattgaata ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa
    16441 tctgtttatt taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc
    16501 gtaaagaata tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta
    16561 ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa
    16621 atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag
    16681 cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt
    16741 atgagaacag atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt
    16801 tatgttggag atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt
    16861 catgcattag gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac
    16921 tctatattat tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat
    16981 aacaaaaatc ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta
    17041 gcatttggga gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa
    17101 acttttattc gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat
    17161 gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac
    17221 agcgtgagat cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca
    17281 gatacaccgc tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa
    17341 gttgataagt tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg
    17401 cagttcttct atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc
    17461 aatcagatta ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta
    17521 atgttggaaa tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg
    17581 gaagtaattg tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac
    17641 atccaaacat tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc
    17701 atgctagtat ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc
    17761 aatgaactga ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca
    17821 atgttgttag aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa
    17881 caaattgtca aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa
    17941 aaattagatg aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg
    18001 gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa
    18061 tgggataatt ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat
    18121 tgaggagttg aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt
    18181 agcgatccac gatgcaatca aggcagggac agatgaacga gattactata ttgataacta
    18241 tatacgaaca atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa
    18301 accaattttt ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc
    18361 tcttttatac gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat
    18421 acacagccag tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt
    18481 cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac
    18541 ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca
    18601 tccagatgat ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga
    18661 cttgaagaaa attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg
    18721 atctttaggg gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg
    18781 catccatttt gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac
    18841 ggcacatccc tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca
    18901 agaagggttc gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc
    18961 tatgcctgga tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata
    19021 tgaggcgata agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca
    19081 tctttgatat ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa
    19141 aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc
    19201 aaacagaaat ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata
    19261 tcaaatggcg agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag
    19321 tatatacgaa aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata
    19381 caatcgtggc ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc
    19441 tatatgcctt gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac
    19501 cagagatctt tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat
    19561 ttgaagactc gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca
    19621 tcaaagatca tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg
    19681 taagtttata tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac
    19741 gatttttaga aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt
    19801 atttattgtc ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag
    19861 tatctacctt atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct
    19921 accagtagat gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt
    19981 atgcagattt ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat
    20041 tggtattttc tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat
    20101 attgtatatc tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa
    20161 aggcaacgaa cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt
    20221 ttcatagtta agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac
    20281 aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca
    20341 atgaaattta tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa
    20401 tgagtaatct attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt
    20461 gcctatcaaa tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta
    20521 tttatgaata acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact
    20581 ggtgaagatg gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa
    20641 actttagaag aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa
    20701 gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa
//
LOCUS       DAZKMB010000045        20757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000045 DAZKMB010000000
VERSION     DAZKMB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.045"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG8V3_001436"
     CDS             352..1041
                     /locus_tag="ACG8V3_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0299981.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG8V3_001437"
     CDS             1028..2236
                     /locus_tag="ACG8V3_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0299982.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG8V3_001438"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG8V3_001438"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0299983.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8V3_001439"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8V3_001439"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0299984.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG8V3_001440"
     CDS             complement(6339..7034)
                     /locus_tag="ACG8V3_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0299985.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8V3_001441"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8V3_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0299986.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG8V3_001442"
     CDS             complement(8233..8424)
                     /locus_tag="ACG8V3_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0299987.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG8V3_001443"
     CDS             complement(8445..9335)
                     /locus_tag="ACG8V3_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0299988.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG8V3_001444"
     CDS             complement(9322..10089)
                     /locus_tag="ACG8V3_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0299989.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8V3_001445"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8V3_001445"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0299990.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8V3_001446"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8V3_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0299991.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8V3_001447"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8V3_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0299992.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG8V3_001448"
     CDS             14660..15043
                     /locus_tag="ACG8V3_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0299993.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8V3_001449"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8V3_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0299994.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG8V3_001450"
     CDS             complement(15987..16805)
                     /locus_tag="ACG8V3_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0299995.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8V3_001451"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8V3_001451"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0299996.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8V3_001452"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8V3_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0299997.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8V3_001453"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8V3_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0299998.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8V3_001454"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8V3_001454"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0299999.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5949 a         4225 c         3807 g         6776 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaat
//
LOCUS       DAZKMB010000046        20360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000046 DAZKMB010000000
VERSION     DAZKMB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.046"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACG8V3_001455"
     CDS             291..1430
                     /locus_tag="ACG8V3_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0300000.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACG8V3_001456"
     CDS             complement(1497..2072)
                     /locus_tag="ACG8V3_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0300001.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACG8V3_001457"
     CDS             complement(2077..3051)
                     /locus_tag="ACG8V3_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0300002.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACG8V3_001458"
     CDS             3334..4302
                     /locus_tag="ACG8V3_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0300003.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACG8V3_001459"
     CDS             4415..4693
                     /locus_tag="ACG8V3_001459"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0300004.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACG8V3_001460"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACG8V3_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0300005.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACG8V3_001461"
     CDS             5150..5905
                     /locus_tag="ACG8V3_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0300006.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8V3_001462"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8V3_001462"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0300007.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8V3_001463"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8V3_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0300008.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8V3_001464"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8V3_001464"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0300009.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8V3_001465"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8V3_001465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0300010.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8V3_001466"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8V3_001466"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0300011.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACG8V3_001467"
     CDS             10642..11184
                     /locus_tag="ACG8V3_001467"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0300012.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8V3_001468"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8V3_001468"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0300013.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACG8V3_001469"
     CDS             12777..13679
                     /locus_tag="ACG8V3_001469"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0300014.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8V3_001470"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8V3_001470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0300015.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACG8V3_001471"
     CDS             15122..15544
                     /locus_tag="ACG8V3_001471"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0300016.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACG8V3_001472"
     CDS             16013..16243
                     /locus_tag="ACG8V3_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0300017.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACG8V3_001473"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACG8V3_001473"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0300018.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACG8V3_001474"
     CDS             17654..17830
                     /locus_tag="ACG8V3_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0300019.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8V3_001475"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8V3_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0300020.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACG8V3_001476"
     CDS             18325..18708
                     /locus_tag="ACG8V3_001476"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0300021.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACG8V3_001477"
     CDS             18877..19761
                     /locus_tag="ACG8V3_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0300022.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACG8V3_001478"
     CDS             19875..20111
                     /locus_tag="ACG8V3_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0300023.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="ACG8V3_001479"
     tRNA            20199..20279
                     /locus_tag="ACG8V3_001479"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="ACG8V3_001480"
     tRNA            20286..20357
                     /locus_tag="ACG8V3_001480"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DAZKMB010000047        20207 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000047 DAZKMB010000000
VERSION     DAZKMB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.047"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..1599)
                     /locus_tag="ACG8V3_001481"
     CDS             complement(40..1599)
                     /locus_tag="ACG8V3_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0300024.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1754..3988
                     /gene="pcrA"
                     /locus_tag="ACG8V3_001482"
     CDS             1754..3988
                     /gene="pcrA"
                     /locus_tag="ACG8V3_001482"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0300025.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4005..6041
                     /gene="ligA"
                     /locus_tag="ACG8V3_001483"
     CDS             4005..6041
                     /gene="ligA"
                     /locus_tag="ACG8V3_001483"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0300026.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6147..6452
                     /gene="gatC"
                     /locus_tag="ACG8V3_001484"
     CDS             6147..6452
                     /gene="gatC"
                     /locus_tag="ACG8V3_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0300027.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6452..7924
                     /gene="gatA"
                     /locus_tag="ACG8V3_001485"
     CDS             6452..7924
                     /gene="gatA"
                     /locus_tag="ACG8V3_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0300028.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7921..9351
                     /gene="gatB"
                     /locus_tag="ACG8V3_001486"
     CDS             7921..9351
                     /gene="gatB"
                     /locus_tag="ACG8V3_001486"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0300029.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9361..10416
                     /locus_tag="ACG8V3_001487"
     CDS             9361..10416
                     /locus_tag="ACG8V3_001487"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0300030.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10527..11900
                     /gene="rlmD"
                     /locus_tag="ACG8V3_001488"
     CDS             10527..11900
                     /gene="rlmD"
                     /locus_tag="ACG8V3_001488"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0300031.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11903..12031
                     /locus_tag="ACG8V3_001489"
     CDS             11903..12031
                     /locus_tag="ACG8V3_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0300032.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12471..12659
                     /locus_tag="ACG8V3_001490"
     CDS             12471..12659
                     /locus_tag="ACG8V3_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0300033.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12737..13750
                     /locus_tag="ACG8V3_001491"
     CDS             12737..13750
                     /locus_tag="ACG8V3_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0300034.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13817..14851)
                     /locus_tag="ACG8V3_001492"
     CDS             complement(13817..14851)
                     /locus_tag="ACG8V3_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0300035.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            14966..17191
                     /locus_tag="ACG8V3_001493"
     CDS             14966..17191
                     /locus_tag="ACG8V3_001493"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0300036.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17209..19092
                     /locus_tag="ACG8V3_001494"
     CDS             17209..19092
                     /locus_tag="ACG8V3_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0300037.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19073..20047
                     /locus_tag="ACG8V3_001495"
     CDS             19073..20047
                     /locus_tag="ACG8V3_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0300038.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6806 a         3709 c         4336 g         5356 t
ORIGIN      
        1 tagatgaata gttgttgtaa aatagttttt ccatgttcgt taaaatgatt gcttcaaatt
       61 tgtgatagaa gatttagcct cgatttcctg cttgattctt ggcaagtttg cttcatcagt
      121 caagataacc aaaatatctc ctacattcat gatcgtatca ccgtgagtca agatttcctg
      181 atttcctctg cgtatagaga ttagcagcat atttttaggc caattgaagt ctcgtaccat
      241 taatccgtct aaagaactct ctgccgttac tggaaaatca aaagttgtcc ttgcaccttt
      301 tagtgaggcg gtctgagtat ttccaagtag acgttccaat aatgattcgt aaattggatt
      361 accgccgaga aggtcattga cgatataagc aacaagagca gccaatccta aaggcatcag
      421 atgattaaga gagccaacca tttctgtcac taaaataatt gctgtcagcg gtgcttttcc
      481 aatcgctgtg aaataaccag ccatggcaat aacgacaaaa tccttgatgt agattggatc
      541 catcccgaaa tcaaccataa tgtttccata aaagctaccg attaaagccc ccaatgtcaa
      601 tatcggaagg aaaatccccc ctggaagacc cccaccgtaa gaaatcatgg aaaaaacgaa
      661 tcgaagaaca aacaaaccga taatcataat gattggcaag gaagcatgtc caaaagctaa
      721 aacgatttga ttgcctccgc ctagagtact aggccataaa taaccaattg gcaaaatcag
      781 caagaaagga acgaacccgt gaaaataagc tggtaaaggc aatcggttgt aaaattttgg
      841 caaagccagc aagacattct gataaataaa acctaagatg cctagaagta ctcctaagat
      901 cactaaggaa ccataatatt tcaacggcaa agaaggaagg tcacctagat ataaaacagg
      961 tctcaagccg aaaaagtaaa gagagacgaa gttagcagtg attgctgaag caaatgatgt
     1021 cagccaaaca agtggagaaa aggagtgatg gatttcttct aaaacaaaga gtaatcctgc
     1081 gataggagca ttgaatgctg ctgcaagtcc gctacttgca ccgctagaaa ttaaaatctt
     1141 ttcttctgaa gatggactcc gaaacaattg tccagctcct tgtccaatgg atgcgccaag
     1201 ctgtatagaa ggtccttctc tacctagaaa aaggcccgca ccaactgaaa gtacaccgcc
     1261 aatgaacttt ttccataaaa cagagaacca gttcattgat aattccccgc gaagctgtcc
     1321 cactacttgt ggaatcccgc ttcccttgat atccggttca tttttgacca gataacccac
     1381 gaaaaatgct gcaaggatgc agcaaatagc taggccaatt aagtaaatcg gctgttcccg
     1441 actcatttca tagatacttc gaactaaatc gcttagcttt tcgatagata atcgaaacag
     1501 gctaacgacg attcctgtca gcagtccaac aaatattcct tttagaataa aatatatctg
     1561 agtcttatct aatttcttga tttttccttt tttttccatt caaacgcctc gctttctttt
     1621 aatttagcgc gaaatcaacg aattgacaag gaaaaatcca taaaaagaaa caaatgattt
     1681 tgaactagct agtttaatga aagccaagaa aatgctataa ttaacaagat tgataaaaga
     1741 aaggatgttg ttaatgtcat ccaaagctga attattaaac gggatgaacc cccgtcagaa
     1801 agaagctgtt cttcacactg acggaccatt actgctaatg gcaggtgcag gaagtggaaa
     1861 gacgagagtg ctgacacacc gcattgccta tttgattgag gaaaaagaag tcaatccttg
     1921 gaacatccta gcgattactt ttaccaataa ggctgcaaaa gaaatgaaag aacgtgtcaa
     1981 tgcgattctc gcatctggag gcgaagatgt ctgggtatcg acttttcact cgatgtgtgt
     2041 gcgtatttta agaagagatg ttgattttat cgggtataac cggaacttta caatcatcga
     2101 ttcttctgaa cagctgacct tgatgaaacg gatactaaaa gaactgaaca ttgatccgaa
     2161 aaaatatgat cctcgaagta tattaggaac aatctctcaa gcaaaaaaca gcctgcagac
     2221 ccctcaggat tttgcaaaaa tgcaaggcag ttattacgaa gagattgcgg caaaatgtta
     2281 tgctgcttac caaaaagagc ttcaatataa tcaatgtatg gactttgacg acctgatcat
     2341 gaatacgatt cgattatttg aagaacatcc tgattcactc acgtattatc aaaacaaatt
     2401 ccattatatt catgtagatg agtaccaaga tacaaatcac gcacagtata cgctagtcaa
     2461 tttattggca ggtagattta ggaacctgtg cgtcgtggga gatgcagatc aaagtattta
     2521 cggctggcgc ggtgcagata tgcagaacat cttggatttt gaaaaagatt atccagatgc
     2581 tgcagtaatt ttgctagaac aaaactatcg ctccacgaaa aacatcctta gcgcagcaaa
     2641 ccaagtcatt gaaaacaata gcaatcgaaa accaaaaaac ttgtggacag aaaacaaaga
     2701 aggaaacaag atcacttact accgtgcaga taatgaacga gacgaaaccc gttttatcgt
     2761 agaccggatg caagaagaga ttcgaagcaa tcatcggaat tacggcgact ttgccatctt
     2821 gtaccggacg aacgcacaat ctcgtgtaat ggaagaaact ctgttgaaag caaatattcc
     2881 ttataaaatg gttggagggc acaagttcta cgatcgtaaa gaaatcaaag atattttagc
     2941 gtatttgaat gtgctggtaa atcctcaaga ttcaatcagt tttgagcgta tcgttaattc
     3001 gcccaaacga ggcatcggac caggatctat cgaaaaacta cgcagttttg cttctcttca
     3061 tgaatggcca ctgcttgaag cagcgcaaaa tgtggatctg gcaaatatcg gtggaaaagc
     3121 aggacagcaa ttaggtgcat ttggagagat gatccaagaa gtcactcaga tgatcccata
     3181 cttgacagtg acagaattga cgaaagaggt tttggatcgt agcggatatc tagaagactt
     3241 aaaaatccaa aatacattag aagcacaagc acgcatcgaa aacttggagg aatttttaac
     3301 ggtcacacaa gaattcgaca aacaattcga acagcaaaat gaagaagacg ctgatgcacc
     3361 agaagaaaaa ctaacggtct tcttaaatga tctggcactt gtttccgaca ttgacaattt
     3421 agaagaagat gcttcccaag tgacattgat gactttgcat gcagccaaag gattagaatt
     3481 tccagttgta ttcctgattg gtctagaaga gggggttttt cctttatctc gagcattgat
     3541 ggaagaaagc gaactggagg aagaacgccg tctcgcatat gtggggatca cgcgagcaga
     3601 agaagcgttg tatttaacaa atgctttctc cagaacatta tatggacgga cgcaatataa
     3661 ccgaccttct cgttttgtag aagaaatcga tcaggagtta ctggaaatcg aaggcatgcg
     3721 tcctacgcct aaaaaaacgc ctgtattcgc taaaaaaaca gcatatagct ataaacagcc
     3781 agagacagcg gttgttccct ctaaaagtgc tactggaggg gaaaataaca gctggaaacc
     3841 tggagataaa gtcaaacaca aaaaatgggg tctaggaaca gtcgtccgag taagcgggac
     3901 gtcgaaggat ctagaactag atgtggcttt cccaagtcaa ggagtaaaac gtcttttggc
     3961 tgcctttgcg ccaattgaaa aagcttagta gtggagggta aatgatggaa catgaaatga
     4021 cgattgacga agctgctaaa cgagcagaag aacttcgtac aagactgaac caatggtctc
     4081 gcgaatacta cgtagaagat aaaccaacag tggaagacta tgtttatgat aaagaatacg
     4141 cagaattagt agcaatcgag gaacaatatc ctgatttgat cacgtctgat tctcccactc
     4201 aacgagtagg cggaaaagta ttagaaggtt ttgaaaaagt cactcatgat attccgcttt
     4261 acagtttgaa cgacgttttc agcaaagaag agctgatagc ttttgatcaa cgtgtacaaa
     4321 aagcagtggg acgtgtagtt gactattgct gtgaactaaa aatagatgga ctttccgttt
     4381 ctttgcgtta tgaagatgga aatttcgtga gaggtgcgac tcgcggggat ggaactgtag
     4441 gagaaaacat cactgaaaac ctaaaaactg tccggtcggt cccaatcaaa ttaaaagaac
     4501 caatgaacat cgaagtaaga ggcgaatgtt tcatgccaaa acggtcattt gtccaattga
     4561 accaagacag agaagcagag ggaaaagata tctttgcgaa tcctcggaac gccgcagctg
     4621 gttctttgcg tcagttagat tctaaaatca cagcgaaaag aaatctggac acttttttat
     4681 acacagtagc tgatttcggt cctatgcaag caaaaaccca gtatgatgcg ctagaagagc
     4741 tagaaaaaat tggattccac acgaatcggg aaaaacgtct atgtcattcg attgatgaag
     4801 tgtggtcata cattgaagaa tatcatgaca aacgtgtgga tttgccctat gaaatcgatg
     4861 gaatcgtcat caaagtgaat gaattctcgc tacaagatca acttggtttt actatcaaag
     4921 caccgagatg ggcagcagct tacaagtttc cgccagaaga agttgaaacg ctaatcgaaa
     4981 acatcgaatg gacagtcggt cgaacagggg tagtaacgcc aacagctatc atgacaccag
     5041 tccgagtagc aggaacgaca gtaagccgtg ccagcctgca taacggtgat tatattaagt
     5101 tgaaggatat ccgcttgaaa gatacagttc tgatttataa agcgggagac atcatcccag
     5161 aagtttcgca agtcgtgtta gataaacgac ctaaagacag cgaagaatac caattgccta
     5221 cacattgtcc agtttgtgga agtgaacttg tccatttgga tgaagaagta gccttacggt
     5281 gcatcaatcc taaatgccct gctcaaatga aagaaggttt gaaccacttt gtttcaagaa
     5341 atgcgatgaa tatcgatggg ttggggccgc gcgtactaga acagatgtat gataaaaaac
     5401 tagtagcaga tgttgctgat ctttacaaat tgacagaaga agagttattg acgttggaca
     5461 aaataaaaga aaaatcagca aataatattt tgacagcaat cgacaacagc aaagataatt
     5521 cggtcgagcg gttgattttc gggttaggaa tccgacatgt cggagcgaaa gcagcaaaaa
     5581 ttttagcaga acatttcggt gatctagaaa cattgagtaa aagtgactat gagtcgatta
     5641 ttgcgcttga tacgatcggt gatatcattg ccgacagtgt agtcacttat ttcagtaacg
     5701 aagaagtcca tgaattgatg aatgaattaa aacaagcagg cgtcaatttc gaatacaaag
     5761 ggcttcgaaa tgcccaattg caagaagtcg agtctccttt taaagaaaaa acagtagtcc
     5821 ttactggtaa actaacacga tttactcgtg aagaagcaaa agagacgatc gaaaatcttg
     5881 gcgggaaagt gactggtagc gtatcgaaaa aaacggatat cgtggtagca ggagaagatg
     5941 ctggcagcaa gctgaccaaa gcacaagaat taggaatcga agtttggaca gaagatcaaa
     6001 tggcagatgc attggctaaa agtcgctcag tagaagaatg aaaaatgagt tttttcagac
     6061 aaatttcaat aaacaatttc agacacggca ctcttgtgtt aaaatagaga gcaaagtttt
     6121 taactcgttt agaaagaagg ggtagtatgg cgattagtga agaacaagtg aaacacgttg
     6181 ccaaattagc aaaacttgcg ttcgcagatg aagaacttgc aagttttacc gaccaattag
     6241 gtaaaatcat cgatatggtt gaaatgctgg aagaagtaga tacggaaggt gttccattta
     6301 cgtcgaatgt ggttgaaaca gtaaacgtga tgcgacaaga tcgggcgcaa gctggatgga
     6361 accgcgatga attattgaaa aatgtgccgg aaacagaaga cggctttatc aaagtacccg
     6421 caatcatcga taatggggag gctggcgcat aatgacagaa ttacacaatc gttctctaga
     6481 agaactgcat catatgcttg tatcgaaaga aatcactgta caagatttga caaaaacaac
     6541 atttgaacga atcaaagaaa cagagcctga ggtcgaggca ttcattacat tgaacgaaga
     6601 aaaagcattg gaacaggcaa aagctttaga tgaaaaaggg attgatgaaa gcaacgtcct
     6661 tgctggtatt ccaattggta tcaaggataa tatcgtaaca aaagatatct tgacaaccgc
     6721 tgcatctaag atcctttaca attttgaacc aatttatgat gcaacggtca tggataaaat
     6781 ctatcaagca gatatgatcc ctgttggtaa gctaaacatg gatgaatttg ctatgggtgg
     6841 aagtacagaa acttcttatt tcaagaaaac aaaaaatgct tgggacccaa caaaagtacc
     6901 tggtggctct tctggtggtt ctgcagcagc agttgcagct ggacaaattc cagtttcttt
     6961 aggtagtgat actgggggaa gtatccgcca accagcagct tttaacggca tcgtagggat
     7021 gaaacctact tatggccgtg tctcacgttt tggtttgatt gcctttgctt catctcttga
     7081 ccaaatcgga ccaatgaccc gtacggtgaa agataatgca ttagcattga cagctatcag
     7141 cggttatgac gaaaaagacg gtacttcttc aggcgtttca gttcctgatt ttgcagaggg
     7201 actgacaggc gacatcaaag gaatgaagat tgctttacct aaagaatact taggcgaagg
     7261 tgtggcccct ggcgtaaaag aagctgtatt gaaagcagca gaaacattcc gttcattagg
     7321 tgctgttgtg gaagaagtca gcttgcctca ttcaaaatac ggtgtggctg tttattatat
     7381 cattgcctca tcagaagcaa gttccaattt gcaacgattc gatggtatcc gctacggtta
     7441 tcgctctgaa aatgtgaaat cattagatga tgtatatgtc aactcacgtt cagaaggatt
     7501 cggtgaagaa gtaaaacgcc gtatcatgtt aggtacattc tccttaagcg cgggttatta
     7561 tgacgcttac ttcaaaaaag caggacaagt ccgtacattg atcaaacgag attttgaaaa
     7621 agtctttgca gattatgact tgatcatcgg gccatcttca ccaacagttg cattcggaat
     7681 cggtgagaac attaacgatc caatcacaat gtacatgagt gacatcttaa cgatccctgt
     7741 caacttggct ggacttccag gtatgtctgt accggcaggt ttatcagaag gattaccagt
     7801 tggattgcag atcatcggta attattttga tgaaaaaacg atgtatcaag cggcatatgc
     7861 gtttgaacaa gcaacagaat tccataaaca acaaccagct attttaggag ggaaagacga
     7921 atgaattttg aaacagtcat tggactagaa gtccacgtcg agttaaaaac caactcaaaa
     7981 attttctctc cagcgccagc tcattttggt gcagaaccaa acagcaatac aaacgtgatc
     8041 gactggggtt atccaggcgt gctgccagta atgaataaag ctgcgctgga attcgggatg
     8101 aaagcagctt tggcattaaa ctgtgaaatc tctaaagata ctcattttga ccggaaaaat
     8161 tacttttacc cagataatcc aaaagcatat caaatttccc aatttgatca accaatcggt
     8221 catgacggat ggatcgaaat cgaagtagaa ggtaaaaaga aaaaaatccg gatcgaacgt
     8281 gtccatttag aagaagatgc tggaaaaaac atgcatggta ttggcggtta ttcttacgtt
     8341 gatttgaacc gacaaggcac gcctttgatc gaaatcgttt ctgaagctga tatgcgttca
     8401 ccagaagaag catacgctta tctagaagca ttgcgatcaa ttatccaatt tacagaagtc
     8461 agcgatgtga aaatggaaga aggctctatg cgctgtgatg ccaatatttc tcttcgccct
     8521 tatggacaag aagagttcgg tacgaaagca gaattgaaaa acttgaactc tttatctttc
     8581 gtgaaaaaag gtttagcttt tgaagaaaaa cgtcaagcaa aagttcttct ttcaggagga
     8641 gaaatccaac aagaaacacg ccgttttgac gaaacaacga ataaaacagt tttgatgcgt
     8701 gtcaaagaag ggtctagtga ttatcgttac ttcccagagc ctgatatccc gaaattcgtg
     8761 atcgatgacg aatggattca aaaagtaaaa gctagtttgc ctgagatgcc agcttctcgt
     8821 cgcgaacgct atatccgtga gtttggactt ccagagtatg acgcaatggt attgacactt
     8881 acaaaagaaa tgtctgattt ttttgaagct gttttggaaa atggtgcaga tgccaagcaa
     8941 gcatctaact ggttgatggg tgaagtttct gcttacttga acagtgagaa actggaatta
     9001 gctgagacaa aattgacacc tgagaactta gcaggaatga ttcgtttgat cgccgatgga
     9061 acgatcagct caaaaatcgc caaaaaagtc ttccgtgaat tgatcacaaa tggcggagaa
     9121 gcccaagaag tagttgaaaa aaatggttgg gtgcaattat ctgatccgaa caaactactg
     9181 ccaatcatta atgaaatttt ggataacaac caacaatctg tcgatgattt caaaaatgga
     9241 aaagatcgtg cggttggctt cctagtcggt caaatcatga aagcaactaa aggacaagca
     9301 aatcctggtg tcgtgaataa gctacttaaa gatgaattag cgaaacgata gaggctgaaa
     9361 atgaaaaaag cacgcgtaat ttataacccc acctcgggaa aagaattatt gaaaaaaaat
     9421 ttggcggata tcctacaggc attagaagaa gcaggatttg aagccagcgc ctatgcaact
     9481 acaccagaac cagattcagc caagaatgaa gcatgtagag tcgcagaact aggttttgat
     9541 ctagtagtgg cagctggtgg cgatggcaca atcaacgaag tggttaatgg aatcgctccg
     9601 cttgaacacc gtcctaaaat ggccatcatc ccagctggta caacgaacga ctatgctcga
     9661 gcattaaaaa ttcctagaga taatattaaa gcagcagcag aagtgataaa aaaacaccaa
     9721 accgtaaaaa tggacatcgg cctttctgat aacagttact tcatcaatat cgctgcaggt
     9781 ggctatttga cagaattgac ttatgaagtt ccatcagaac taaagagtat ttttggttat
     9841 cttgcttatc ttgcgaaagg agcagagatg ctccctcgag taaagccaat caaaatgcgc
     9901 ttgaagtatg atgaagggga atatatcgga aatgcttcga tgtttttcct agggctgacg
     9961 aactctgttg gaggattcga acagatcgcg ccagatgcca aattggatga tgggaagttt
    10021 tctttaatca ttgtgaagac agcaaatatc tttgagatct tgcatattgc agcactcatg
    10081 ctgaatggcg gtcgacatgt cgatgatcca agaataatct ataccaaaac aagcagtttg
    10141 ttcgttgaaa cagaagatga tatcaatcga ccagtaatga tcaacttaga tggtgaatac
    10201 ggcggggaag caccgatgca tttccaaaat cttcatcaac acattgagtt ctttgccaat
    10261 accgatcaga ttcctgatga agcaatcaca ggtacagatg aagaagaatt agaggttgtc
    10321 agcaaagaat tcgtaaaaga agtcgaacgt ttaacggatg aagatatcga cggtgacgga
    10381 aaaatagcag acgaaacacc taatcaaaag aattaatcaa aagccggtgc tgaacgtagt
    10441 aaggtcagtg ccggttttag ttgtgtaatt agctgattta tagtagagtt cagtaggatt
    10501 gaaaaatagg cttaaaggag caaaatatga cagaacagca aatagtaaag aaaaacgaac
    10561 gatatacagt cgaaatcgtc gacttgagtt atgaaggaat gggggtagca aaaatcgatg
    10621 gttatccatt atttatcgaa aatgcactcc ctggagaaga agtagaaata ctagtagtaa
    10681 aagcaggcaa taagtttgga tatggaaaag tcgaaacatt tattacacaa tcacctgatc
    10741 gtcagcctgt agaaaacaat gcattacttc gcataggtat cgcaccttta gcccatttga
    10801 agtatgaaca acagcttgtc ttcaaacaaa aacaagtaga gcatgtaatg gcgaagattg
    10861 ctaagatgcc agaagttcct gtacttccaa cggtcggaat ggcagaacca ttcggctacc
    10921 gtaataaagc acagatccct gttcgccgta tcgatggaca gttagccacc ggtttttaca
    10981 aaaagaatag ccatgatcta gtccctatcg aagattttta tatccaagat cctgtcatcg
    11041 atcaagcaat caaagtagtc agagatatct tgcaacggtt ccaagtacga ggatataatg
    11101 aagaaaaaaa tgaaggacag cttcgtcaca tcattatccg tcgtggtcat tattcccatg
    11161 aaatgatggt cgttttagtt actcgtaaag aaaaattctt taaatccaag gaaattgctg
    11221 aagctattca tgaagaattg ccagaagtgg tttcggtcat ccaaaatatc aatgaagaaa
    11281 aaacaaatgt gattttagga gacaaagaaa aagttttata tggcagagac tatatcgaag
    11341 atcagctatt aggaaaaacc tatcgaatct cagcaaagtc attttatcaa gtaaatactc
    11401 aacaagcaga gtatctgtac caaacagcca tcgagtttgc cgagttagat aaaaaagaca
    11461 ttgtgatcga cgcttattca ggaataggta cgattggctt gtctttagca gacagagtgg
    11521 ataaggtgta tgggatggaa gttattccag aagcaattga agatgcacag ttcaatgctt
    11581 taaataataa aatcgaaaat gttcattatg aagtcggaaa agcagaaaaa gttatgaaaa
    11641 aatggcaaga aaaagggatc aaaccatctg tgatcgtagt tgaccctcca cgaaaaggat
    11701 tagatcatca ttttattgaa tcagcaatcg aaatgtcacc agaacgaatc gtctatatct
    11761 catgtaatcc ggcaactttt gcaagagatg ctaagttatt tgcagaagca ggttatgccg
    11821 tgaagaaagt tcagcctgta gatcttttcc cgcaaaccca tcatatcgaa ttagtcgctt
    11881 cattcgttaa aggagaataa aaatggacca aaaagaaaaa atgaaagaat tgatcaaaaa
    11941 gaaacaaggt ggaggccgat ccaaacaaat ggaaacacct aagaatgatc gtaaaaacat
    12001 gagaaaaggc cctaaaatct ttaataaata gacgtgctaa agaaagacta agaagtgtta
    12061 acttttgtta ttgtttctta gtctttttag ttagtctaca ttattttggc gatttgccaa
    12121 taaggactgt tgagttgtgc aaaatcaaat ctgggtcaga atgaacgtca ttctgtctca
    12181 gatttttcgt tatttcaatt tgattaactg attgatcaaa aagattttta ttttcatatt
    12241 ctcaaatgat tcaacactaa atatacatgt ttgacgcgga aattattatt cgattttggc
    12301 ggcatcaggg gggatgacga cgtagcagac ccattatttt actcaagaaa atgtccgtgc
    12361 ctattattta gaatacgata gtgaacgtgc tgggagtttt gttcttttag agaaagtctc
    12421 tgaagataaa ttagttgtct tagattttga ctgtaacgaa aaatggcgaa atggaagagc
    12481 accagcaagt gattgaccgg atctacgaag cgacacgata tctgcctttg gatcgtttgt
    12541 gcttaagtcc tcaatgtgga tttgcttcaa cagaagaaga aaatgttttg tttgaagaac
    12601 aatggaaaaa atttcttttg tcaaaagcat tgcagaagaa gtttggggaa aatcattaaa
    12661 tcatctagga gagcttgata ggaaataaca ctatggtata atcagaatgc ttaatactaa
    12721 ctactgaggg agccttatga aaaagaaaat tcttaaattt atcggatggt tcttcggaat
    12781 cattcttggg atcgcgtttg caatctttct tgccttccag ctttccccca gacctggagc
    12841 attcttgatc aaccatatgt tttcaaatac tgttcaaatc actgataaaa aaacgttcga
    12901 tcaagcagaa aaaaacgtgg cgaaaaaaac agatttgacg tatgagtcca agtataaaga
    12961 gaatacttat gacatctact atccaaaaaa cagcaagggg cctgttcctg ttctattttg
    13021 ggtacatggt ggaggattcg tcggcggaga taaatcgggt gtcaaagaat ttgcgacaaa
    13081 attagtttca gacgccaaca tcgcagtggt tgcgatgaat tatgaattag cgcctgattc
    13141 agaataccct aatcaagttc ttcaagtgaa tgaactgatc aaagatttat tagctgaaga
    13201 aaaagacgac aaattgctag acatggaaca attatttttt ggtggagata gtgcaggttc
    13261 acaaatcgca ttgcaatatg cagctataca aacgaatgat gcgtatgcta aacaaatgaa
    13321 aatcaaacag ctacttccaa aagatagtct gaaaggaacc atttcttatt gcggacctgt
    13381 tgatttgaag ctaacagcac agcagcattc ggatgatcga tttatgaagt ttttcgtcaa
    13441 aaccgttgcg tggtcgttga ttggaacaaa agattggaaa acgagtgagc aattgaaaga
    13501 agctagtgtg gctgaccatg tttctaagga atttccgcca acgtatataa ctgacggaaa
    13561 tgcatactca ttccaggagc aaggtcaagc attagaaaat cggttgacag aattaaaggt
    13621 acccgtgcaa tccttgtttt atacagatac aaaaaaagag attacccatg aataccaatt
    13681 tgattacacg ctaaaagaat cgcaaaattg ttaccagcag acattggatt ttgtgaataa
    13741 gtataagtaa gaggttgtga aaaagccgtt tcgttttgag tatgaagaaa cgaagcggct
    13801 tttcacaacc tctctttcaa tacctttttg ttcttgggat aaaactttcc ctaacaatca
    13861 atcgtgcagg ataagtcaaa atgattggtt cgtcccgtat accgtcaatt cgctccaatg
    13921 ccattcgaac agctgcttgc ccaattgatt tcgcattgat ttgaacactg gtcaagccag
    13981 gggagaggtt tgcgattgtc tcgatattat caaagcttgc tacaacaatc tcttctccaa
    14041 tttggatatt tttttgtttc aatgcccgat agacaccaat tgccattgga tcgctcccga
    14101 caagaatggc agtaggaaga ggggattcat tttctaatag attagtcgca agtacctcac
    14161 cttgtttttc tgtccagcca cctagatgat aagacatata tttttctaag tgatgatcga
    14221 tcataaaatg ctgataggca cgcatcctct tttcatttat agaaatctgc ttcccaccga
    14281 attcatccat atcgacttta tatcccccga tataggctat tcttttatga ccattttcta
    14341 agaacaattc cagtacggtt ttagtcatcc gttcaagatc tgcatacacc atatcgacct
    14401 cttgctggat gtcagggtta tccacgacga ctatttgcat gttttgtttt aacaaggcgg
    14461 taatcgattc tttcgtaaca gtcccaacaa cgatcaccgc acctaactga tctagatctt
    14521 cccattgttt aggattatca gatagattat agattcgatt catccctaaa tgcagacgtc
    14581 gagcttcttc ttccatccct actcgcaaat gtttgtaata gatatcctgt tcttcgctcg
    14641 ctttgccgat cgtagagatc acaccaatcg aaggcattct ctttttgata tatttacgag
    14701 tactgatgtc ataatccaac gcagcagctg cctctactat ccgttgccga gtagtctgag
    14761 ccacatggat cgtctgatcg ttattcaaca cacgggaaac tgtagcagga gagacatttg
    14821 cctttcgtgc gacatctcta attccaacca tttttacaca ctcctcaaaa aaataaaaaa
    14881 tttttattga caaaattgtt tagtaaacag tatacaggaa ttataaaaaa aagtaaacgg
    14941 tttccgtttt gttgggagtg aaatgatgga tattacgcat agaaaaacag ataaaaattt
    15001 catcttattt gatgaaaaga gccgagtctt ccatttacga aatacatttc tttcttatct
    15061 gataaagata gaagaaagca atgtattggc gcatatttat tttggcaaac cagtcaagca
    15121 gtacaaagac aataaaaatt atccccgaag agatcgtgga ttttcaggaa atgttccctt
    15181 aaatcctgac cggtctctgt cgaaagatac actaccgcaa gaatactctt ctcatggcag
    15241 catggacttt cgaacgcccg catcgatcat tcagcgcaag aatggatcag acttactgga
    15301 tctgagatat gacagccatt atattacaga tgggaagcca gagatcgaag gcttgcctca
    15361 aacttatgtg atggacaagt cagaggcaca aacgttggtg atttcactta aggatagaga
    15421 aacagcgatt tatttcgatt tgttctatac catatttact gatagagcag tgatcacacg
    15481 atctgtaaag ataagaaatg aaaccggaga aacaatcaaa ctagaaaaag cggctagttt
    15541 ccagctagac tttgcccata ctaggcgatt tgatgaagtc attgctttgc caggagcaca
    15601 tgtcaatgaa agacagattt cacggcaatc tgttttatct ggaacaaaag tatttgaaag
    15661 ccgtcgagga acgagcagtc accatatgaa taactttatt gctttagttc atcatcatac
    15721 gacagaaaat actggagaag cgatcggact tcaatttgtt tattccggca accattcttt
    15781 cgaacttgaa aaagatcaga tcaatcaatt gagagtcgtc ggtgggatca acagccatcg
    15841 tttttcgtgg gaattgaatg caggccagtc ttttcaaaca cctgaaatga tcctttctta
    15901 ctcaagtcag ggattgaaca aaatgagtca gatccaccat gagttgctac gtgaaagaat
    15961 cgcaagaggt cgtcatcaat ttgctgaacg gccgattctg gtcaataact gggaagccac
    16021 ttactttgac ttcaatagtg aaaaaatcaa ggcgatcatt gatgaagcaa aggagctagg
    16081 catcgaaatg ttcgtattag atgatggctg gtttggaaaa agagatgcgg acaattcttc
    16141 attaggagac tggttcgagt atgaaggcaa gttgacaaat ggattacgtg aaatagcaga
    16201 ttatgctcat agcaaaggat tgaaatttgg tttatggttt gaacctgaaa tgatctcggt
    16261 tgattctgaa ctgtatcgaa cacatcctga ttttcttatg caggagccag gtagaatgcc
    16321 ttctgcttcc agatcacaac atgtactgga ttttacaagg ttagatgttc ggcagacgat
    16381 tgagaaacaa atgagaaaga ttttagatac gattcctctt gactacatca aatgggatat
    16441 gaaccgttcg ctttcagatg tctattcgat tacccttgac ccacagagac aaggagaagt
    16501 agcgcatcgt tatatgctcg ggctttatga actacttgaa catttgacaa ctgattatcc
    16561 ggaaatctta tgggaaggct gttctggtgg aggtggacgg tttgatgccg ggtttattta
    16621 ttatatgccg cagtcatgga caagtgataa cacggatgca gtggagcgta tgaaaatcca
    16681 atatggaaca agccttgctt atccgatttc gtccatcaca gctcatgtat cagctgtacc
    16741 aaatcatcag accgacagat ctacttcgtt gaaaacaaga ggagaaacag ctatgagcgc
    16801 tgtttttggt tatgaactgg atttgacaaa gctgtctcct gaagaagaaa agcaagtaaa
    16861 agaccaaatt atatcctatc aaacgattcg tccggtgatc cagtatggcc attattatcg
    16921 tttggcgagt ccttttgaag aaaatattgc cgcatggatg tttgtgtctc ctaaacaaga
    16981 tgaagctatt gtgtttttag ggaggatact agcttcagct caaccagcgt tccatgaagt
    17041 atatctgatg gggttagatg atgaggcact ttatcaggaa cagacctcga agcggatatt
    17101 ttcgggggcc gaattgatga cagttggact ttacttcccc gattttcaag gtgatttcca
    17161 aacagaactg cttcatttca aaaagttatg agagagaagg aaaaaagtat gaaagcaaat
    17221 ataaaatggt tagatgatcc acaagttttt agagtgaacc aatcaaatgc acatagcgat
    17281 catgtttgtt atgcaacaag ggaagaagta gaaaccacat cctcccttaa acagtcttta
    17341 aatggatcat ggcgtttttg ttattcgcca aatccttcgg tacgaccaaa aacattttat
    17401 aaaaaagatt ttgatgcctg ttcttttgat gaaatcactg ttcctagtca tattgaatta
    17461 gcaaattatg acaagatcca ttacatcaat acgatgtatc cgtgggaggg acatgtattt
    17521 cgccgtcccc ctttcacgac aagccctgag aaagaggcgg aagggatctt ttcagctgct
    17581 aaagacaatc cggttggttc gtatttgacg acttttcgat tagaagaagg tctaaaggga
    17641 aaacaaatct cgattcattt tgctggtgta gaacaagcaa tgtttatttg gttgaatggt
    17701 cattttatcg ggtatgccga agattcgttc actccgtctg aatttgattt aacaccttat
    17761 ttggaagaag aaaatattct agctgtggag gtttacaaaa gaagtacagc tgcattcttg
    17821 gaagatcagg actttttccg attttttggg atcttcagag atgtcttctt agttgctcga
    17881 ggacaaaacc atttagaaga tctttggatc aaacctcgct tagatgacaa atgtcaaaat
    17941 ggcaaacttg aagtagattt gtcttttgag caagtagcca aaaacagtca gctgaatgta
    18001 gcgatactag atcctgatgg gaaggaaatc tatcaacagc aatatccgtt aaaagagcaa
    18061 atgacgattc gcatcgatgg atttgaaaat gtggcactgt gggggcattt tcagcccaat
    18121 ttatataagc ttgaattgga gacgatagac gaagaaaaaa gagtaacgga gtatattgac
    18181 tattccttcg gttttcgtaa aatcgaaatc aaagataaaa tcatctactt caatggaaaa
    18241 cggatgatta tcaacggggt aaatcgacat gagtggcatc cgaaaaaagg acgcgccatc
    18301 actatagaag atatggaaaa agatttggct atcataaaaa atgcaaagat caacggcgta
    18361 cggacatctc attaccccaa ccagatcact tggtattatt tatgcgacca agcaggaatc
    18421 catgtgatgg cggaaacgaa cctcgaatcc catggctcat ggcaaaaaat gggcgcattt
    18481 gagccttctt acaacgtacc aggtgcagta cctcagtggc aggcggctgt tcttgatcga
    18541 gcaaaaacga atttcgaaac attaaaaaac catccaagta ttcttttttg gtctcttgga
    18601 aatgaatctt atgcggaaga aaacattcga caaatgaacg attacttcaa aaaagtagac
    18661 cccgatcgac ttgtccatta tgaagggaat tttccaaatc cagcattcga agattcgatt
    18721 tcagatgtaa aaagctgcat gtatgcgcca ccagaagaga tcattgacta ccttgaaaat
    18781 aaagcggaaa aaccgttcat cttatgtgaa tatatgcatg acatggggaa ttcgttaggt
    18841 ggaatggatt cttacatgac gttgcttgat cgttatgaaa tgtaccaagg aggctatata
    18901 tgggatttta aagatcaggc gatccaagtg actgatccaa tcacaggcag agaagtgatg
    18961 cgctatggcg gtgactttga cgataaacca agtgactatg aattctcagg gaatgggatc
    19021 gtttttgcag atggacaaga aaaacccgcc atgcaggagg taagatatta ttatgaaaaa
    19081 tacagtaaat aaaagtcata tggatacgga aaaagttgca atcgtcttcg gcgactgtac
    19141 attaggtgtc aaatcgggga atacgcatta tattttttct tatacaagag gcggactgga
    19201 atcgctcaat aaaaacggaa aagaatggct ataccgggaa acacttccag ttttttggcg
    19261 agcaacgacc gataatgaca gaggtagcgg atttggattt aaatccggta tgtggttagg
    19321 agccggattg tatccaaaag tcaaagaaat agaagtactg gtggacaata aaaagatcga
    19381 tttgccgatt gctccagtaa ataatcaata ttcaagtaat gaatacgctg aaacggttga
    19441 aatcattttt acactggaaa tcaacacagt accaaaaacc caagtgacaa tttcctacca
    19501 aatcgctgga gagggtaaaa tgcttgtcaa tatgcattat tatggacaaa aaggattacc
    19561 ggaattaccc gcattgggtt taaggttcat tatgccaacg aaagcaaaat tttttgagta
    19621 tgaaggccta tcaggggaaa catatccgga tcggatggct ggtggtgtcg aaggtattta
    19681 taaagtggca ggactccccg tttccccata ccttgttccc caagaatgcg gcatgcatat
    19741 ggaaacgaaa tgggtagaac taacaagaaa tcaaacgttg gataatacgg ataaagatca
    19801 agaagatttt tcgttgcgat ttgaacagca ggatacgcca tttgcttttt ccgctttacc
    19861 attcaaagca gaagaactgg aacaggcaac acatatagaa gaactaccgc cagaaagacg
    19921 aacggttctt acgatttttg caaaagtaag aggagtcggg ggtatcaata gctggggagc
    19981 ggatgttgag tcaccttacc gaatcagcag cgaagaggac catgaaatcg gctttattat
    20041 tcagtagcaa tctttagatt attatttgaa aaacggagtt tacaagacaa agctgcttta
    20101 ttctatatgt catgaacaag agagtaaaaa agttctcaga tacttttgtt catgactatt
    20161 ttcttcaata aaaccaattg ttatttttct gaaaaacaaa gttaact
//
LOCUS       DAZKMB010000048        19644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000048 DAZKMB010000000
VERSION     DAZKMB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.048"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..665
                     /locus_tag="ACG8V3_001496"
     CDS             18..665
                     /locus_tag="ACG8V3_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0300039.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            749..1693
                     /locus_tag="ACG8V3_001497"
     CDS             749..1693
                     /locus_tag="ACG8V3_001497"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0300040.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1828..3279
                     /locus_tag="ACG8V3_001498"
     CDS             1828..3279
                     /locus_tag="ACG8V3_001498"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0300041.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3300..4136
                     /locus_tag="ACG8V3_001499"
     CDS             3300..4136
                     /locus_tag="ACG8V3_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0300042.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4136..4795
                     /locus_tag="ACG8V3_001500"
     CDS             4136..4795
                     /locus_tag="ACG8V3_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0300043.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4921..5610
                     /locus_tag="ACG8V3_001501"
     CDS             4921..5610
                     /locus_tag="ACG8V3_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300044.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5616..6572
                     /locus_tag="ACG8V3_001502"
     CDS             5616..6572
                     /locus_tag="ACG8V3_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0300045.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6616..7128
                     /locus_tag="ACG8V3_001503"
     CDS             6616..7128
                     /locus_tag="ACG8V3_001503"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0300046.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7384..7932)
                     /locus_tag="ACG8V3_001504"
     CDS             complement(7384..7932)
                     /locus_tag="ACG8V3_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0300047.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8021..9202)
                     /locus_tag="ACG8V3_001505"
     CDS             complement(8021..9202)
                     /locus_tag="ACG8V3_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0300048.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9438..10946)
                     /locus_tag="ACG8V3_001506"
     CDS             complement(9438..10946)
                     /locus_tag="ACG8V3_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0300049.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11105..11296
                     /locus_tag="ACG8V3_001507"
     CDS             11105..11296
                     /locus_tag="ACG8V3_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300050.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11499..12416
                     /locus_tag="ACG8V3_001508"
     CDS             11499..12416
                     /locus_tag="ACG8V3_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0300051.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12409..13482
                     /locus_tag="ACG8V3_001509"
     CDS             12409..13482
                     /locus_tag="ACG8V3_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300052.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13630..14148
                     /locus_tag="ACG8V3_001510"
     CDS             13630..14148
                     /locus_tag="ACG8V3_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300053.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14129..14521
                     /locus_tag="ACG8V3_001511"
     CDS             14129..14521
                     /locus_tag="ACG8V3_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0300054.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14597..15532
                     /locus_tag="ACG8V3_001512"
     CDS             14597..15532
                     /locus_tag="ACG8V3_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0300055.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15665..16444
                     /locus_tag="ACG8V3_001513"
     CDS             15665..16444
                     /locus_tag="ACG8V3_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0300056.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16437..16754
                     /locus_tag="ACG8V3_001514"
     CDS             16437..16754
                     /locus_tag="ACG8V3_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0300057.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            16998..18608
                     /locus_tag="ACG8V3_001515"
     CDS             16998..18608
                     /locus_tag="ACG8V3_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0300058.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18765..19613
                     /locus_tag="ACG8V3_001516"
     CDS             18765..19613
                     /locus_tag="ACG8V3_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0300059.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6347 a         3514 c         3956 g         5827 t
ORIGIN      
        1 caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt gcagctttgt
       61 ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg tcttccagta
      121 gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca tcttcgtcta
      181 gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg tctgcagctc
      241 gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt ttggaattag
      301 atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa aaagaatacg
      361 aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa actctagatc
      421 gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat aaattgatca
      481 cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg gcaacagatt
      541 gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa gacggcaatc
      601 aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact gaacaagatg
      661 attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat acactagaca
      721 aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg ccaatcatat
      781 ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc aggaattaga
      841 tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga tcaatgaacg
      901 aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca attctatggg
      961 acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg aaaaagtgca
     1021 ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc aggaaaactt
     1081 ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat taaatttatc
     1141 ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca cttatgaaac
     1201 actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc ctccttattt
     1261 tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat tgacctatgt
     1321 caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag aagcagtagt
     1381 catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc cgactgcttt
     1441 ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca ttggtacagg
     1501 tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa gcatgttaca
     1561 gattggtaca gagcttcata aagaagggcc ggaaattttt tctagaatca taaaagaatt
     1621 aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag gcaaattacg
     1681 caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt ttataaaaaa
     1741 cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa cttgtgggaa
     1801 aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc aaaagagctt
     1861 aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa aataaagaag
     1921 caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa ttcccacgag
     1981 ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc tttaaaaatg
     2041 ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt tgaaacacac
     2101 cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct agttcctttt
     2161 ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt catctatggc
     2221 ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg aggagctgct
     2281 gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg tggttctatt
     2341 agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg gacgatgcca
     2401 gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg cttgacagtt
     2461 tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc cggtgcgcca
     2521 tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt gaagatagct
     2581 ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact tgctgtaaaa
     2641 aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc ttacccacta
     2701 gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac agcagcaatg
     2761 atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact tgaacccatt
     2821 tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat cgattctctg
     2881 catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata cgatctattc
     2941 ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca aagtgatgcg
     3001 ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt agacgttgtt
     3061 tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc taatctgact
     3121 ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc tctaggaatc
     3181 caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta tctatttgaa
     3241 gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg agaagaagaa
     3301 tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta agcgcttgcg
     3361 gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa aagaaagaat
     3421 tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat caaaatgaat
     3481 tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat gtcaaaatct
     3541 cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga gaatcagatt
     3601 ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc ctttcagaga
     3661 ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca ggaatccact
     3721 cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc tatatgcgtt
     3781 tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat gatcagtatt
     3841 taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat ctgcagaaaa
     3901 tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt tatttcaatg
     3961 aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag aaaatcgatg
     4021 gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa aaatattttc
     4081 aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt gagtgatgta
     4141 ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac tgcaaggctt
     4201 ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg tagggttgct
     4261 tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc gtttttattt
     4321 atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt tcgggttacc
     4381 ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca gcagtgcatt
     4441 tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt gggacgctgg
     4501 tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc cgcaagcttt
     4561 tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag gtacttcgtt
     4621 agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag gcggtcggac
     4681 attcgattat ttttctctct atattttagt tgcactgatt tattgggtca tttgtatcgg
     4741 gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa tctgaaaaat
     4801 agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta tattctgcct
     4861 agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga ggtagaaaac
     4921 atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg catcgagcaa
     4981 ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt agatacgctt
     5041 agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct gaaagtgaca
     5101 aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa ttattttgtt
     5161 ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat aggtgtttta
     5221 gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt tctgattgcg
     5281 aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa ccgcttcatg
     5341 gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt aaaatatttt
     5401 ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga tccattttct
     5461 aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat caatgagttg
     5521 caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca acagcagatc
     5581 cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat ttaagaggaa
     5641 aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc tccaataaaa
     5701 tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg atcgaattat
     5761 ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga caattcaaat
     5821 tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc gtccctgcac
     5881 agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg tttcttgttt
     5941 tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca ggagttgatt
     6001 cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa aacaaaattg
     6061 tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat aaaacagatg
     6121 acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga ttttctgatt
     6181 tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg cttgctatcc
     6241 atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc tctgacagta
     6301 acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa atgaaaaaat
     6361 ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa ttattggctc
     6421 tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca cttatgcatc
     6481 atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa tattattcaa
     6541 aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa aaaggaatag
     6601 aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga agaagtggta
     6661 gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca atggcaaaac
     6721 gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga aagctatgta
     6781 ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt ggatcccgtt
     6841 tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc gattcatcgt
     6901 gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt agaacatttg
     6961 ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta tccagctaat
     7021 gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat gattgaattt
     7081 ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact ataaaatcat
     7141 ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat agccaagagg
     7201 ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat gcttctcaaa
     7261 tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc ccttcatcga
     7321 gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta ttattcgata
     7381 catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg ctccatcgtt
     7441 tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta aatcttctac
     7501 ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt tcaggcgcga
     7561 ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc cgaaatactc
     7621 atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta ctgtcagttt
     7681 ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt cccactctgc
     7741 aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat aataatcctc
     7801 ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct tttgttccgg
     7861 ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga taaatacata
     7921 gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc tttggaaaat
     7981 atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc aagccagtca
     8041 caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat ccgtgttttg
     8101 cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg atcccgatag
     8161 aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta cgctcagtaa
     8221 aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta accagtttag
     8281 tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg atgaaggcaa
     8341 gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc ccgtggaaga
     8401 tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca accgtgatcg
     8461 ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc tgttctttgt
     8521 tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt acaattgaag
     8581 ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat ttagaatcga
     8641 tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc gatacactca
     8701 tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa ataaaaactt
     8761 ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg cccatcccgt
     8821 tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc ttgatatagc
     8881 gtagaatacc gataatagca gaaataaata caattggcat caatacggaa agaaagaaag
     8941 gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc ccagcaaaag
     9001 caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca aaagttgtac
     9061 gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt ggtttcaatt
     9121 tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca aataaaccaa
     9181 taatacctaa taaataactc aaataatcac tcctattcca aattccgttt tattttacct
     9241 gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta gagtgaaacg
     9301 cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa gtgtaacgtc
     9361 ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg ttttcaacat
     9421 ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac aggtgcgcag
     9481 gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac catcacagta
     9541 gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg agcgattgga
     9601 ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag tgcatccaaa
     9661 gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa tggtgacaat
     9721 aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat aactgccgct
     9781 gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg taattcgatt
     9841 gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc tgcatagtca
     9901 tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc cataatcagt
     9961 ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca ttctgtttgg
    10021 aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc atccacgatc
    10081 ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga ttctttacca
    10141 gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg attatagttc
    10201 acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac atcttttaga
    10261 tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac attccatact
    10321 gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc atgatacagt
    10381 tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac tacaacgatt
    10441 ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac gattttttct
    10501 acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt tcgtctttct
    10561 agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat aacgacactg
    10621 ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc catatcataa
    10681 gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata gacgcaaatc
    10741 agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc ttcttcaaat
    10801 cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa ttctaaatat
    10861 aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc aatatatttt
    10921 tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa gcattttttc
    10981 atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca aagaaaatgg
    11041 tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat aaggaaggtg
    11101 ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc gttcgatttt
    11161 taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt ttacttatct
    11221 aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca caacaagaga
    11281 tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt cctatcagat
    11341 ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc tgttttatgg
    11401 agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat tgaagtatta
    11461 ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca gatacaaatg
    11521 caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca ggacaagaat
    11581 cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa ttattagaag
    11641 acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc taccgtttat
    11701 acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac gagatcgatc
    11761 aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt atggaacaag
    11821 tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac catgatcggc
    11881 ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag atcgatgaag
    11941 agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc aatgagctag
    12001 acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa ataaaagaaa
    12061 tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt aaaaaaatcg
    12121 aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa aaattaggct
    12181 ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca ttgaagcaag
    12241 aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg caaatcagac
    12301 gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg atgaattatc
    12361 ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat gaatgaacac
    12421 gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga aacgaacact
    12481 tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc tatgctagag
    12541 gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac tgcagaaaac
    12601 acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga gcaagatcat
    12661 caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt acgtatgaga
    12721 aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg ttttatattg
    12781 aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt cacgtatgaa
    12841 ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta tgacacatgg
    12901 aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt agtcttttcc
    12961 tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct cctaaacgta
    13021 tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt gatccgacac
    13081 tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca caaattgaat
    13141 caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt actagacaat
    13201 acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga agtattggat
    13261 gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat caacgaaact
    13321 acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa cgaaaaagat
    13381 caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga tttctttatc
    13441 aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa gttggattac
    13501 cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca gaataattgt
    13561 cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga aggagggatt
    13621 tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc tgcttgtgta
    13681 gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc tattttttta
    13741 tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct ttctattgcg
    13801 atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat cagctatact
    13861 gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag gaatctatgg
    13921 ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat gaccgttttt
    13981 gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg ggtgtcgttt
    14041 gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc agtcgtcaaa
    14101 cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt tgcttgcaat
    14161 gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat cggcttccaa
    14221 ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag aaaatggtca
    14281 aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga gtattttgaa
    14341 acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag ggcactcaca
    14401 ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg ctactactgg
    14461 tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt ctaaactata
    14521 agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt tcagttgatg
    14581 aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg taattgctgc
    14641 tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa tcagtggcag
    14701 tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc agattttgct
    14761 gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat ttggtgaagg
    14821 acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg gcggtggggc
    14881 acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag tgggtaatcc
    14941 gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt cgttacgcgt
    15001 ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc gctttttgcg
    15061 cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg ctgtaatcgg
    15121 tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg ctgcatttga
    15181 tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt tcctagcaaa
    15241 agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat caaaaatcaa
    15301 gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag ttcatttaag
    15361 tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag agaatgaagc
    15421 ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg acaagaagat
    15481 gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat aaaacgttta
    15541 ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa aacggctaca
    15601 atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta aaggagatat
    15661 tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag tgatgccact
    15721 ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa aagttggatt
    15781 tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg cgcagttttt
    15841 agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga tggttttctt
    15901 tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga aaaaagaaaa
    15961 gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg cggtgaacta
    16021 cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg tcaattggtt
    16081 ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct cggtgattcg
    16141 agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca tgtttatcat
    16201 gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat taggggtcgt
    16261 attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca ttgcttcatt
    16321 tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac taagagaccg
    16381 cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca atcaccatga
    16441 ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat atcccgcgct
    16501 tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt gactggatga
    16561 aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc attactcatg
    16621 aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta gcaggcgtgg
    16681 cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct gtctttttgc
    16741 tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat aagttgttca
    16801 aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt tattcgtagg
    16861 gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag gaattagaga
    16921 gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt tatgaaagaa
    16981 tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg ctaacctttg
    17041 ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta agtatgatgg
    17101 ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat cttccttatg
    17161 gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa aaagtagctg
    17221 tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt acgatcaata
    17281 tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct cctttactag
    17341 ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga agtttatgcc
    17401 catctttgat tgctatccca attcttagtg caatgagagg atatttccaa ggaaaaaatg
    17461 atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg atcgttatct
    17521 tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa gctgtattga
    17581 tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg tatatcgtcg
    17641 gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt tatttcgaaa
    17701 gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt atttttgtcg
    17761 gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca ctgcttcact
    17821 ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt gcttctgtca
    17881 atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct ataagcagct
    17941 tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa acagtaggag
    18001 atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg tctttattag
    18061 ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga tatttccaaa
    18121 tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg atgcttcaat
    18181 ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc ctgaaagtca
    18241 tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta tcaaatggct
    18301 tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa taccgaatag
    18361 cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc ctgattatgc
    18421 ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg gaatcaaaaa
    18481 gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta tttttatttt
    18541 ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat agcagaagca
    18601 aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact cgtatgatta
    18661 cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact tatgatatag
    18721 ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa cttgcagtag
    18781 ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac ttattcgtta
    18841 ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat atcgaagcgg
    18901 atgaatatta tggactttta aaaaacagta aagattttcc tactacttca caacctgtcg
    18961 taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac acgattttaa
    19021 gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg atgaaagatg
    19081 acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg ccgatgggac
    19141 acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat gaaatacttg
    19201 cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat gatttgaaca
    19261 atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc ttattaaaaa
    19321 tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa atccgttcaa
    19381 gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac gaaatacaac
    19441 cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt gcggagaaag
    19501 agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc ggaccagtca
    19561 ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa taattccttt
    19621 tattcaaaaa acataccaaa aata
//
LOCUS       DAZKMB010000049        19614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000049 DAZKMB010000000
VERSION     DAZKMB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.049"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8V3_001517"
     CDS             complement(<1..132)
                     /locus_tag="ACG8V3_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0300060.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..781
                     /locus_tag="ACG8V3_001518"
     CDS             542..781
                     /locus_tag="ACG8V3_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300061.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            848..1057
                     /locus_tag="ACG8V3_001519"
     CDS             848..1057
                     /locus_tag="ACG8V3_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300062.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            1117..1788
                     /locus_tag="ACG8V3_001520"
     CDS             1117..1788
                     /locus_tag="ACG8V3_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0300063.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1841..3817
                     /locus_tag="ACG8V3_001521"
     CDS             1841..3817
                     /locus_tag="ACG8V3_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0300064.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3830..4585
                     /locus_tag="ACG8V3_001522"
     CDS             3830..4585
                     /locus_tag="ACG8V3_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0300065.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4582..5406
                     /locus_tag="ACG8V3_001523"
     CDS             4582..5406
                     /locus_tag="ACG8V3_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0300066.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5416..5664
                     /locus_tag="ACG8V3_001524"
     CDS             5416..5664
                     /locus_tag="ACG8V3_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300067.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5707..7806
                     /locus_tag="ACG8V3_001525"
     CDS             5707..7806
                     /locus_tag="ACG8V3_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0300068.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7844..9901)
                     /locus_tag="ACG8V3_001526"
     CDS             complement(7844..9901)
                     /locus_tag="ACG8V3_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFQ0300069.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(10015..10227)
                     /locus_tag="ACG8V3_001527"
     CDS             complement(10015..10227)
                     /locus_tag="ACG8V3_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300070.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            10414..10881
                     /locus_tag="ACG8V3_001528"
     CDS             10414..10881
                     /locus_tag="ACG8V3_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300071.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10878..11120
                     /locus_tag="ACG8V3_001529"
     CDS             10878..11120
                     /locus_tag="ACG8V3_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0300072.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            11138..11440
                     /locus_tag="ACG8V3_001530"
     CDS             11138..11440
                     /locus_tag="ACG8V3_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300073.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            11512..13566
                     /locus_tag="ACG8V3_001531"
     CDS             11512..13566
                     /locus_tag="ACG8V3_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300074.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13566..16178
                     /locus_tag="ACG8V3_001532"
     CDS             13566..16178
                     /locus_tag="ACG8V3_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0300075.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            16175..18706
                     /locus_tag="ACG8V3_001533"
     CDS             16175..18706
                     /locus_tag="ACG8V3_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0300076.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18758..19090
                     /locus_tag="ACG8V3_001534"
     CDS             18758..19090
                     /locus_tag="ACG8V3_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300077.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            19091..>19614
                     /locus_tag="ACG8V3_001535"
     CDS             19091..>19614
                     /locus_tag="ACG8V3_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300078.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILG"
BASE COUNT         7048 a         3061 c         3901 g         5604 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaatagta
      541 gatggcgcga ccagttttag aggcgatgga tatggatact ttgacaaaag gaaggtatgt
      601 acagctgtta gctcgtggtt attcacctaa agaagtattc aaagccttat ctaaaggaac
      661 agatatggaa aaagaaagat tgcgaaaaga attcgattat tggagaaccc ataatggaat
      721 caaggatttg aaaccagcac ggccagtaat taaacgaaag aaaaagataa cccaagactg
      781 aagtgtagag cgtatgtgat cataggcttt tttatttttg caaaaaacaa ggaaggtagg
      841 aaaactcatg aattatgaag aaatcgaaaa cagaaaaaaa gtgtcgaaag aaatggaaga
      901 aaagttacta aaaacgatga agcaaaaaca ccttaaaaga ttatctgtcg cgcaatatat
      961 caatgatatg cagttaacag gaaaagagaa agcttgtttg ttaggttcaa tgaaaaactt
     1021 tgaacagttg agacgaactt atagaattca tttttaaaac tttttctatg gaaatgagaa
     1081 aaagtttttt tgttttgtct gaaaggaggc agatggatga aaaaatggtt atttggtttt
     1141 ttaggagtca ctttaatcgt cttttgcagt gtgtttggct atgttagcta ccaaaaacat
     1201 gaaggagagg tgtttaagca aaatatagaa aaaaagatgc ccgtagatca aatcaatgca
     1261 catgcaaaga gttataagga ggatgcaacg aacgtcaata atgatatgtc tttggggcaa
     1321 atgttgtcca tacaaaaaga agcaatcgaa atgggcgtca ataaacaagt atttgcacaa
     1381 attcagattc ctgcacttgg tttagcattg ccaattttta aaggggcaaa tcaatacact
     1441 ttgagtcttg gagcggctac ttatttttat gaagatgccg aaatgggcaa aggaaactat
     1501 gtcctagcag gtcataacat ggaaatgcca ggtgttttat tttctgatat tcaaaagttg
     1561 tcattagggg aggtgatgga cttagttagt aacgatggcg tctatcgtta caaggtaacg
     1621 cgtaaattta tcgtacctgg gtactttaaa ttaatcgatg gcgttcctga agagaactct
     1681 ttcttgtctt tgccaaagaa aggagagaaa cccttattaa cgttgtttac ttgtgtttat
     1741 acgtcacaag gaaaagaaag atacgtggta cagggagagc tacaataaca aggttttaat
     1801 caacgcgagt cgttgaaata tgaaatgagg agagtaaaaa atggcaataa atcggtatgt
     1861 aaaaaaagct ggaatgcttg ttggaatgtt aggaatcatt gcaggctgct ttcaggggta
     1921 tcaagtacat gcagaagatg tcacgcaaaa aacaccacca gagaaggtca atatcacggt
     1981 tcataaatta atgtacgatc aaggaacgca attgaatgtt gatattgacg ggatcaaaaa
     2041 cgatggttat acgcacgatc aatatcctac aggtgtaacg aaatacaaca aggcagatta
     2101 tggtgatgtt gagtttacgt taggaaatat tacggatcaa gtattaccga gagaagattc
     2161 tgatctaacc aacgcgaaag tggacgagat tgtacaagat gtagaagaca agggatctaa
     2221 cagtgagtac gtcaagaatg caaccaacaa aattacttca gctgtagatg aaaatggtga
     2281 gatcactttt actgatcaac cagcctatgt caattccaaa gggaatgtat atgtggttta
     2341 tgaaagtaaa tccgctgctg gattagtcac acaaaaagca aaaccaatgg ttgtgattgc
     2401 gccaatgaca gataaaacag gatcaggatt tttaaaaaat attcatttgt atcctaagaa
     2461 cattgtttct aagttgagtt ttgaattaac gaaatttggt gacgatggcg cagaccaaag
     2521 caaacaaacc cttttaaaag gggcaaaatt tgagttatac aagggcgaac ctggaaaagg
     2581 aacaaaattg ggtgatcttg tatcggatga tcaaggaaag ctgactgcga ctgatttgac
     2641 gttaggaaaa tactattttg tggaagttcc ttcggaagtt gtagtgggat cagatcaaga
     2701 accaacagca gatcaatatt tattaggcgc agacgctcgc aacgatgcgc acaataaatt
     2761 aacctttgaa attacgaatg atggcgtgac aagtgactta aaagcttcat acgtaaacta
     2821 taaagcacca gtgatcgata aaacagtaac aaatggaaca ggacaagaac attctttcca
     2881 aattggtgac gcagtaaatt atcaaggaac gattcatatt ccaacagaca ttgcaggtgg
     2941 cgcagacggt atcacagtaa acggtgtgaa aagcgaaact tctccttaca gtgtatttaa
     3001 atggggagat acagcaggta aaggactttc ttatgttgct gaaaaagcag atattaaagt
     3061 aacaaataat gatggttcag tcgtattaaa agaaaatact gactataaaa tcgaaaattc
     3121 tgatagtggt tttgtgatcg attttatcgt aaataatgga caagtaagtg atacagttgc
     3181 gaatttacat ggtcaagatc taaaaatgac ttataacatg tttgtgaatg atagtgctga
     3241 aatcgcaaat ccattaacaa atagcgtgga tttcgtttat aacaataatc catttaatca
     3301 ggaagaacac cacgaaaaaa cgaaagctga cgttgtgact tatggcgcaa aattcttaaa
     3361 agtcgatagc ggcctatttg gaacaggtat caaagcaaca ccgcttgaag gcgcagaatt
     3421 tgcagctaag aatgcagaag gtaagtacta cggtggactt actgatacag ataaagatgg
     3481 tgtcaaagaa gcagtttggg tagacgatgt agcaaatgca gcaatcctaa aatcagataa
     3541 agagggtcat tttgaaatca ctggtttaac tgaaggagat tactcactgg aagaaacaaa
     3601 agctcctgaa aactatcaaa aattaacaaa agagatctct ttcaaagtag acaaagattc
     3661 atacaaagaa gaaaaccgaa tcacgatcaa aaataatcaa aaagcttccg taccaatgac
     3721 tggtagcaac ggtttccaat cttacgtatt gatctcttgc ttgttgctag gagcaggggg
     3781 actttctgca gttgtttact ttaagaagaa agcctagaag gatcagctaa tgattattcg
     3841 taagccccca aaaagaagag tcatgcacaa actgatgata tttttttggt tgctgtcggc
     3901 agtagcagtc tatttaattc cagttgtgga aacaaatgta gcgaaatttc aacaacagca
     3961 aagaatttta gccattgaaa atcttgaaaa aacagattct tctttggatg ttgcgaaaat
     4021 agagttaggc gatcctgttg gtgttcttac aattccaagt atttctttaa aactaccaat
     4081 ttatgacgga acttccgaca aaattttaga gaatggtgta gggattacgg aaggaactgg
     4141 cgatattaca ggaggaaacg gtaaaaatcc tttaatcgcg gggcacagtg gactttataa
     4201 agataatctt ttcgatgatt tgccttcagt aaaaaaaggg caaaaattct ttattaaggt
     4261 aaatgaagaa caacatgcct atcaaattta tcgaattgaa gaagttaaag cagaagaatt
     4321 acaaaaaaat tatttagatt atcttatgcc tgaaaaagac caagatagga tcacgttaat
     4381 gacatgcaca ccaaaaggtg tcaactcaca ccgcttttta gtgtatggtg aacgagtttc
     4441 cttcagtaaa gaagatttaa aactacagaa aaaagcaaaa aataggaaat caatttcatt
     4501 ggattggcga ttagcactgc tattcgcttt tttttgcgtt cctattattt ttatttataa
     4561 aaaaataagg aggagatcgg aatgaataag agaaaatggc tgtttgtttg tgtagcagct
     4621 ctcttttcta ctgtagctta ttcgcaactg tctttcgctg acagcagtga tagtagtacc
     4681 actcaaacaa tagataattc aattactgca ccaagcgaaa cggacagttc ggagaatact
     4741 acagaagaaa cggcagattc aaatataagc tttactgaat caacgaatcc aacgtcagaa
     4801 agttcttcag agacttcaga aaagccgcaa gacgtagaag atacgcaagt gatcaagcaa
     4861 gccattcata ttcaaaaaat tatttcttcg aatcaaatca acaatcaagg cagtcaagta
     4921 gcccagcaag aaggtatcaa tggcgcgaag tttattgtct acgatatcac gaatatctta
     4981 gaagaaatgt cacaagatga taatgagaaa aatcaatctg tagaaataat tgagtcaaaa
     5041 ttgaaagatc gagcaaaaaa actttcatac gaccagttga aaaaagtggc tgaaggagaa
     5101 accaaaacga ttgatgatca ggaaggcgtg attgaatttt ccattgaagt acctgcaaac
     5161 aaaaaacaag catactatat cgttaatgaa tcaagccctg aaaacatttc aaacagtgaa
     5221 gatattgttt tattgacacc tgtttcaaat gaaaaaggtg aatttttgac ggatgtttgg
     5281 ttatacccta aatcagagaa atcagagccg aaagaagaaa ttaaaaaagt tgtgtcaact
     5341 ggtgtaaaga agaacttttt tgaaaattgt tgggaatttt ttgctcaatt attttttgat
     5401 aattaggatg aaatgatgaa aataataaga atattagttt ttaatctatc ctttttagcc
     5461 gcaacaatat tgtgttgcgg tttaattttt tctcaattat caatttatcc aagatatcaa
     5521 cgattgttca ttcattttct aaacattcaa tgtgcttcca tgccattaga ggtgattaaa
     5581 aagctcttga tcaatccaat ttttatggtt agtttggttc ttcttacagg attggtaacg
     5641 tgggtattag gaaaaaaaga atgaaaagct tacgatcgtt taaaaaaata ttagatggag
     5701 ggcattatgg aaaataaaat tcattgcact acgggaagag taattacttt agtgttgttt
     5761 ttgttccttc ttctttttgc gaggggaact gcagtccagg ccttagaaaa agttgatccg
     5821 agtcaaatca aagaaaataa taatctggtg ctttatcatg atagtgattc attgagaaat
     5881 atcgaagtaa ctgggatcaa tggatttaag atactagatg agtattcaac taaaaaacaa
     5941 aaagtacttg cttggccaaa agtcaaagat ttgaaaagta cgtcaaaaat cgttgcccat
     6001 tatcaaaaag taggtactta tctaggaaaa gaaattgatt gtgacgtgac tttttctaat
     6061 tttgtagcgc acgctggggt taaccaagac ggattagcta aaacaattaa tggcaaaaag
     6121 tattataatt atttgctctt tgataaaaat atttatcgag gatttttcat gcagtcaatc
     6181 aatgtcatga atgcaaaact agaatttaag tatgcaggaa caaaaacaaa agtagctttg
     6241 agtgaaccac agccagaaat tcaatcaaaa atgagatcga tgagagcagc tggtattgat
     6301 ttaaacgata catttctatc catttacagt ttaaatggac agtatcagca acgtgtgtct
     6361 tggtataatg acaataatga atctgtcggt gaacgtaata ttgatatgag agaatttgtt
     6421 gggtatgaaa aaatgggtag cttaccttat tttgtcacaa cagatacagc atgtgcagaa
     6481 tacaaagctc ctgcgttatc aacaaacaat ttaacttcaa aagtagtggg aggacgtgca
     6541 gaaaaggctt atagctcgaa tgatcatttc accgatgttg taggagctga tacttatcac
     6601 agaagtggtg taacgtatac gcttcaaggc gcttccccaa cattcatgat tggcgcaaat
     6661 acgaatagta tgatgtttag ctttgatact gcattgctat ggacaccaca accatcgaag
     6721 cctacaaaag aagtgtttaa caaagctaat actgaagagg cagcacacaa tattgacaaa
     6781 aaagtgattc cacaaggatc agatgtttac tatcatattc accaaaagtt tgatgcatta
     6841 acagtcaaca caatgaacaa atacaaatca tttaaaatca ctgatacctt tgacagcaaa
     6901 aattttgata tggtatcgga tgggaaaaac tatgatggcg cattgtggta tgttgaagcg
     6961 aatggtactt ggaaaaaagc tgatacagga ggaaagttaa ctgtatcagg taatacagtc
     7021 acttatactg cttctgacag ctttttagct aacgctgcaa attacggtag ggattatatt
     7081 ttgcagctcc atatgagagc aaaaaatgat gcaaaaggaa ttgcaccaaa tgtggctact
     7141 gtaacgttta acaatgatcc gaacaaagga acacaaaaaa caaatacagt agaaaactat
     7201 ttctctctta aaccaaataa aaaagtggaa caagatggga aagatgttga tggtcgaaat
     7261 gatggtaaaa aaggaacgcc tacagctcct ttaaatgcag gatctgaagt acaatatcta
     7321 gtcacacaaa aatggcatac aaaaggtgta gacattgcct cagatcatta caagcaattt
     7381 agtattcaag atccagtgga agatcgtttg acgtataaag aaggttctgc gcgagtgata
     7441 gatatgagta caggaaaaga tattacttca gaaggtacac taacctatga tagcaattca
     7501 agaacgctga agtgggaagc ttccgctgat ttcttaagta aaaatctttt agatggacga
     7561 gaaattcagc tgatatttac agctaaaact cctttgaatg aagaattgga tatcgacaac
     7621 caagcaaaag tttcagtaga aagtattgag aaagcaacta atgtagtaac gattggggta
     7681 aaacctaact tacctcagat cattgtgcca aaaactggat cgaatcattt agtgctaact
     7741 gttattggaa gtatattatt tcttacttta gcatcattgg gctatgtagt tttaaaactc
     7801 aaataaatat aaaaacaagg ctgagaatca gccttgtttt cttttatttg aagattggat
     7861 tcgcactaat aaatacatta tctacgccaa ttctaccagc agagtcaact acaaaattat
     7921 gacctaatcc gagaatactt tggttattat aaagtaaatt tgcattatgg gcagggctat
     7981 ttttccatcc taccatggca tcgatagcac catcgtctga taaaaggtca tcaatataca
     8041 gcccagtttg acttatgttt tctcccttag caccgtagca gcctaagctt tccaataaat
     8101 cttggaaagt accaccacca gggcgatagt gactgaagct gacttgtaat tctttagctc
     8161 gaatatctgc agcctgttga acaatatcat tgaattgcat tggggcaaca ccattttgtt
     8221 ggcgataatc atttaaccaa gcaagttctt tttgacgcat aaagttacgt aattcgtcta
     8281 atgaaatctt tttatatttg aatgtgattg ttttattttg atcgacgtta ttaatggatc
     8341 gttggccgtt tttatccacg gtaacggtag gatcgtcatt tctgcacaag aaataatttt
     8401 gatcagtcat gtttttccaa ggagtagcaa agctatcccc ttcgtatact gtttttgatt
     8461 cactagagag aacagcgttg gtatcagcat ttacatgatt aacagtcaca ttaaatttac
     8521 gagtgtagtt gaaaatataa ttttgacttt cagtcacatt gtttaaggtt actgaatttg
     8581 ttggttctac ccgatcgtct aaaacatatc cttgaataga aggagcagtt aaagtgacac
     8641 tgtcaccgtg agtgacattg actatctgtt ctttcaaaat attaactcgt ccttcgtttt
     8701 tgtagttgtt attgcacaac aaattgattt gatgcacttg attataattg aatgtgattg
     8761 ttgtattacc tgtgattgtt ttgctttgag tatcattacc aatcactttg tagctaaaat
     8821 tatctttagc gccttcgcta tatttaaatg cttttgcctt agcagtgtaa gtagaaccag
     8881 aagttacttt tgcttgatct gtagcaatca cttgacctgt ttgattgtca atatggttaa
     8941 tagtaaccgt atattctttt ggtgtttcag gtttgtgttg caacccttta aatgcagcat
     9001 caagactatt tttagcattg tttacttgtg tttgagaaac cttagaatcg tctagtactt
     9061 ttttagcatt ggataaagca gtctgaaaat tattccaaga ttcttctgta aaatctcctt
     9121 tagcagttga ttttactcgg ttgtataaag cttgtaattc agctttattc acagtaggaa
     9181 ctggtttttt ctttaatcct ttgtaagcag aatctaattt atttttggca ctatccactt
     9241 gcttttgagt cgcttttgag tcatttaaca ctttcttagc attgtttaaa gcggtttgga
     9301 atgcgttcca ggtattgtcg gtatagtcac cttttttcgt tcccttaacg ctcgtataaa
     9361 gagcttctag agccgattta ttcactttgg gtttagaggt gtcagcgcca taccaaccga
     9421 taccttcgta tttccagcca tagtctttgg ttaaccattg tacttctttc caacttgcag
     9481 tgtagtgatg tgagccagat tttacaccag gaaggaaaag gcggtagatc ggtttattat
     9541 ttttttcggc agaacgccag gcaggccctt cataagtcca gccgtaattt ttagtcaacc
     9601 atttcacttc atcccaacta gctgtgtaat ggtgatcacg aagaccttta ttgtacaaac
     9661 gatagacaag atggccaccg ctagtcggtg cttcccaggc ggggccttca taatttcccc
     9721 attttatctt tactagatgg tcacgttctt ttttgctaga ggtgtaaaaa tgttcattat
     9781 tatttggatt gtaaaggcga tacatgacgt tagttgaagc agcgtcaaca gatccagcaa
     9841 gactaagtga ggcagcaaat gttccaatag ctaaaacagt ttttggatta aataatttca
     9901 ttttttcaac tcctaaattt aattaaaaat gtttacatga ttattatata tcggctaacc
     9961 gatatagtca ataaaaaata agaaaagcct tatataattt tggcttttct tattttattt
    10021 gttagttgaa gtattaatat caacgatttc ttcagccaat ttatttaatt tcatagcatt
    10081 tatgaatctc atgttgtcta tactagagcg tttgttggca aggtcactta ctgtagttaa
    10141 gggtacgcca gattctatgg atatagaacg aactgattta ccactatcaa acagcaacca
    10201 ttcgattttc tttttatcag cgtacatttc aatgctcctt cttgtttttt ctttattata
    10261 gctctttttt gtaatgaatc acaatatcaa gagaaaaatt ttctatagaa aggtggattt
    10321 ttatgagtat tcgttctccg tgtatccaaa tgaggtttgt tgctactttc gttatttgat
    10381 atactaaaaa taaacaacga aagaaggaat aaaatgaaga aaaagcaact atggtttgtc
    10441 tctatgtttg caatattttt tattatttta agtggatgtt caagtaataa gaaatcggaa
    10501 gctaaagaag ataatagttt attgcaacca ggaactgttt ggaaagaaga agtaggtggt
    10561 cttattcata atttaaaaat tatagatgat actacctggg aatattctga aagtgtatgg
    10621 catccagatc ctgtacaaat aacagttaag aggcaaaaag actatgaagg tttagaaaga
    10681 tacaaaatag tagattctgc aggcattagg gaatttataa acaaacctga ttcattattt
    10741 attgttgttc cctatgagaa ggatggcgta aaaaaaataa tttttcttgg atctagcaag
    10801 gatgagaagc gaacgaaaga gaaattaatt catgatggat cagagttaaa taaatacaaa
    10861 ttacaaaaga ctagtgagtg attgctagtc tttttattta tcctgaagga ggaatcacag
    10921 tggcaaaagt taaaacacca ttcgaaaaag ccgcgtctaa aatcttagaa cgccgtggtg
    10981 tttctgaaat acagtggaaa aaagaagttc ttgaaaaagc acaatttgac ctattacgag
    11041 gaaaagataa ggacttagaa gaatatatct atcaacgtga acgtgaaaaa cttgtattcg
    11101 atgaaattat taaacattaa aaggagcgaa taatcttatg tttgaatcaa ttacaatgaa
    11161 ttttttaact caaccgaaag tagtagctgc agttgatatt tttagagcat tacaaactgc
    11221 cgcaactagt ttgaaaaata atctgatgat ttttgcggct gtagctgccg ttgcctccat
    11281 tgctcttgga ggtattttct tcttctttgg gcgaagagga gctgaaaacg ggaagacaca
    11341 cgtaggttat gtattgttgg gggtaggagt tattttagct gccgcttcta ttgtagtttc
    11401 aatgttctca atgtttggac aaaccccaca aggactataa aaaaaacaag taggaggagt
    11461 aaaaagtacg tggctacttt ttactccttt ttttatgaaa ggagaatatt catgtttttt
    11521 acgatttcag gaaatgcaaa attggataaa gagttaataa gaaaagcaac gaagaaagtg
    11581 ggaaagcgta cttccttttt accgaaaaca attaaatatt ctgtcattga aacggaagaa
    11641 cagctacatg tgttaggaca agtgttagaa caagagaatc aatcttctgt taaattgact
    11701 ctcacgttac atgataaaga aggaaaaata gaaaaggctg gcacagaata cgtttttggc
    11761 ccactggatt atcaaatttc agaaggtttt tatcctacat tgatagattt aatccgcctt
    11821 gatgtgttta aagatacgga tgaaactatg agtgtggaag agtcttatcg aaacgaaaaa
    11881 ctgctacaag ttttagaaga agagttagag agcgaccaaa gaaaagtgaa tcaagtgagt
    11941 gaagtcgaag taaatgaaga agtaccatgg gaacgaccaa tctttccaaa gaaggagtca
    12001 aaagaaaaag agacagtcta tgttgacgaa caagctgata ttccgactca gaaatcgata
    12061 tttgaggaag agaacgatgt gatgggtcaa ttggaggtag atccctttga agaaacaatg
    12121 gaggatgaag agacggacga agtagaagtt gatccagttg aagctaatga agagtcaaca
    12181 tggactgaag gggattttca ggaatatatt gaaccactta tcagtgaaga agtagaaaaa
    12241 aacgaaacag agatacaaaa ggcaactgta tttgattata ttttggatca atatccgcca
    12301 gagcatgaat ggatgaaaga aaaaaacaaa ttatttttaa atgagcttta ttcgaaatat
    12361 caattacccc aaacctatca agcattttta gagagtaaag aagaattaat ctcacagggt
    12421 aaaggcacac ttttagaaca gttagagtct gtccaatcaa caaattggca tgagcttgca
    12481 caagaagatt tattagccac ctttgaagaa cgtcaacaag aaacagatca agaaattaat
    12541 gagtatgcga cggaacagtc gtctaattgg gagaaagtaa aagaggaact aaataaagaa
    12601 gaacagcgag tgattgaaga agaagtacaa aaagtcaaag agcggtacga aaagaaacgc
    12661 gacaatgctt attccttgtg tgtagaaaag atcaacaatt ttactaagac gaatcaagaa
    12721 gcgttatcga aagaaaaaga aaacttgtta aaagaacgta ttaaagaacg aaaagaagag
    12781 ttctaccgcg gtttacgtac agataagcta aagattagca aagaattaaa tgaacaactg
    12841 aataagctat atcaaacaac gactgaagtt cttatggaaa aacacgaaca gatccagcaa
    12901 gaattacaag tacaaattcc aaaatggaaa aaagagtatg aaattcaaca acaagaattg
    12961 aaagaacaag aagaaaaaca acggcaaaaa gaaaaagagc gtgaacaatt agaaataaga
    13021 aaacgggaac aggatcttgc ggaacaaaaa atggcgatag aaaaacaacg gttagagcaa
    13081 gaaaaggaaa aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcgt
    13141 caagcacagt tgaattatct agcacaacct caaccagtag ctgctgttca aacgccagtg
    13201 aacaaccaag caaatttaac tccttcaccc cagcgttcct ttaacggatg gatggtggga
    13261 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
    13321 aatcaatctt cacaggctga ttctgtagcc acaacggcgt caatcaagga agaagcccag
    13381 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
    13441 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg atcctagtaa ggctaaagaa
    13501 ggatcaagcg attcaactgt acactcaaac acttccgaaa gttcagaatc aaatgaggtg
    13561 aaataatgcg tattagagaa acaacgagac gaaaagacaa acgattcaat ttagaaaaga
    13621 ataggtatct ttctcatttc tatcgctata ctccttcagg agaagagcta ccagaaaaac
    13681 atgacaaacg tatttctcaa aagatttttt atagtggctt agcttatctt cctttaaact
    13741 atgttgttac aagaatacca gaagcatttc atcgatttga gattattcca ggaagtttac
    13801 aaggagtgaa tatttttcag ttacaagaaa tgccgcagtt attccatttt gattcaactg
    13861 gtttttggaa tcctttgacc ccaacaacaa gtttaggcac acgtttagtt gtttctacga
    13921 tttgtgttgt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtccgctag
    13981 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
    14041 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
    14101 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
    14161 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
    14221 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
    14281 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
    14341 tgagtgataa tccattggca ctcatcgtga aatactggaa acgaggagac atagatactg
    14401 caacacagtt aaccaatact ttcacaaata cgatttatta tgatgccaac gcttcagata
    14461 acaaatactt taacgaaaac gcgcagaaag ctgtcaatgc gcttattttt gcgttgttag
    14521 aacaagctga tcaaacagga gattactcca aattgacacc gaataatctc gttgagcttc
    14581 tttctgagat tggcggtttt aattattatc cagatccaga caatgaattc aaacagttga
    14641 atgccttaga tgaatttatg aatcagttgc cacctggaaa cattgcaaaa aaacaatatg
    14701 gggcaacgaa aattggatct gataaagcga agggaaatat tttatcaacg gcaatcacgg
    14761 gattgaatcc atttactcta acgaaaattg caaagatgac ttctcaatca acgattgatt
    14821 ataaatcggt tgggtttcca aaatacttag atttgaaatt acatgaaagt cttttaaatc
    14881 aacgagtcgt agtagaattt tatcataagg gaaagaaatt tcatgaagaa cgtgtcaaag
    14941 taggctataa aggatttagt gaaatcaatt ttgattgtca tttagttgca ggtgatcaag
    15001 tttgtttaaa atacgatcat caaggaaaag aatatgtggc ttggtatgaa ttttctcaaa
    15061 gagtattgaa agacgaagag ggtaatatcg tttatcggaa aaaacgagga gaaacccatt
    15121 tgccagaatt agaaaaagaa gccgttctaa cattagatga ggaaaaaagt aacttattag
    15181 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg cttacacctg
    15241 attatgatcc atctaaccat atcattgtta gtatttttct tagtcagtta tataaagaat
    15301 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgcaggatt cattttattt
    15361 tagatgaatt tgggaatatg ccaccaatgg ataatatgga aggaatcatg acggtcacag
    15421 ctggccgaaa catgttgtgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
    15481 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
    15541 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
    15601 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
    15661 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggagaa acgattgtac
    15721 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
    15781 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
    15841 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
    15901 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
    15961 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatcttttg
    16021 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca gcagatcgac
    16081 ttcaagaata tgttgaaaag ttgatcaaaa ataaagaaat ttctgaaatg attgggaaac
    16141 ggttgttatt ggaatctgaa aaggtggtga aagaatgatc attggaggaa ttttttctgg
    16201 catagccgac tggtggtcag gcttattcgg agaaggggat gcaaacccca aaaccgtaac
    16261 gaaagtctta caagattatg ggaattatct gcagtatcaa gatgtattta gttggatttg
    16321 gaacactttt ctgtggggat tgattaaagg attatataat ctcaatactt tactagaaaa
    16381 aaccatttat caaagctttg atttaaaaga cttattgaac gccgctgggg taaatgcact
    16441 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
    16501 aggcttgaaa ttttttatga gtaaacaccc accaaaagtc aaaaatatct tggttaattt
    16561 atttatggcc atgatcttga tttttggtgg tagttcttta atcgatgaag ggttaaatat
    16621 cagtaaaagc ttttacggag acgtcactgc agcaaataaa gcagataaaa cgggatcacc
    16681 tgcttttcaa attattcaaa ataatgtgat cgatgtcagt acgttactag caactgaccc
    16741 gaataaagta gaatccctac caacagataa aagaaatgct ttaacagcga aaaattttaa
    16801 aacagcgaat atcaatggga ttattactcc tgaacaagct aaaggtatgg caggtaatga
    16861 taaaagtggg ttatcagata tacaaaagga tcggttaaag gcattacaat accgattgga
    16921 actagatgat aatgggaaag aaattcctgt tcaaattagt gacgaaggct tagcaaaata
    16981 tgtctatact tcagggtatc gtcgatacag tagtaaacca ggtattattt tagctggtga
    17041 atcttctttg gcagtagcct atttatttat cctatttacg atcgttactt gcattattga
    17101 gctggtattt aaaaaattct atttagtgat tgccgcgtca acggactttg agacaggtca
    17161 acggatgaaa agtgcgatag aagatatttc tcaatccttc ttgttattgg cgtttacgat
    17221 tttagaatta cgaatctata ccttaatgct ttcaggtatc ggtgatttgc atgcggcagg
    17281 aaagatcaat ggattcttgt atgtcgttgc tttgattgta ctaaccattg cgctattcaa
    17341 aggctctcaa tcagttacga aaattttcgg cgttgataca agcctgaaaa acggcagtaa
    17401 ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa
    17461 aaaccttgca agtgctggta agagtggttt aagccattta aatgatattc gtaaaaatgg
    17521 atttggccat cgaaaagatg ttacccctga taacggggaa gaacaatcag acgataaagc
    17581 tacaactgta cctaatagta agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg
    17641 tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc
    17701 tgaagatctt acagaaaaag ccaaagacac cttagaagat aaattagacg gcacgagagc
    17761 taaagaattg gctgaagctg tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa
    17821 agcttcagaa gtcaaagaag gactaaaaga tgcgtatggt gaaggacaag tcaatgcagc
    17881 agtgaaagct aatacaacaa ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt
    17941 caaagatcac caagaagcgt tgcactctaa tgaaaattcg attgaagaga aaaattccat
    18001 agaagaaaga aattcagaag aaggaaaaaa tggacagtta ccaaaaactg atccagaaca
    18061 atatcgctat aaacctaaca gtgaccttga aaatgagaag cgaatttcag atttatcaaa
    18121 agtaaatcaa gaagacaaag cagaaaatga gggacagtta ccaaaaactg atccagaaca
    18181 atatcggtat agatctgtta actccgaaaa tgaaaaacgc atttcagatt taccaaaacg
    18241 aaatcaagaa gtacaaacgg atgaggattc catcgttctg ccaaatgata atggaatcga
    18301 agttggaact gaaaaatcaa cgaaaattgg tttacctaag gcacaaaatt taccaatgaa
    18361 agatagtcgt ttgaatcaat tcaaggataa tgatcaaagt tttgttcatc aatcacctgc
    18421 aggtactgtt caaatgaaac caagtcgttt gcctgaaaat ggaaatctga caactgtttc
    18481 ttacaattta tctgaaagtg gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag
    18541 agaagctgca ccaaaaccta ttcaaaacgt tcatacaggt tacaagccag caaatgtggc
    18601 atttactgca ccaacaaaaa taaatgtgac aagagaaaca aaaaacagga acaatgcatt
    18661 gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa ccataaaagg cctacaagtt
    18721 aggccttttt tctatgtatg aaaaggagaa atcatttatg gataaatata gtaacatgaa
    18781 aaaccctcca agatttagtg gagaatggaa gtttttcgct tttttctttt tagatctatt
    18841 aacgcttggt ggctgctttt tttgggcgaa aatggtcaat aatcttgtcc acttaccact
    18901 cttttttgca ctcttgaatt atggcttatg gattgcgata gcgttagtga ttgtttggcg
    18961 accctataat aacccaggtc aacgacaaat ttttgcgatt atcgagtcta ttttttttgc
    19021 ggacaagaac cattatcatg cgttagatcc taaccgaaat tatcatggta cgtataagga
    19081 ggatgaataa atgcgagtca aaggacaaaa ttcagttgaa aggaaatcca ttttgcagtt
    19141 tggaaaacca gaagagcgct ctatcttgga cttatttcca tataaatcct gtacagaaga
    19201 gggagtctta attactagag aagatcattt tcaacgttat tatcgtgtag tttctacaga
    19261 cgtggaagga ttaaatgaac aagagaaact agaacgaatg aatcagttga ccaccgtcat
    19321 gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg atctaacgga
    19381 acaaatcgtg caaaagcggc aattattaga taaaaatcgt atggaacagt ccttaggaag
    19441 aaatctacaa cgattaaaaa aatatgaaaa gaaattagtc gaagaaatcc aggaattgaa
    19501 acgagcagaa caagaacgtc ctgacttaag cttctttttc gtcattgaag gaaagacaat
    19561 caaggattta caaaccagaa accgtcagtt aatgcgatct agtggcattt tagg
//
LOCUS       DAZKMB010000050        19385 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000050 DAZKMB010000000
VERSION     DAZKMB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.050"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            754..1416
                     /locus_tag="ACG8V3_001536"
     CDS             754..1416
                     /locus_tag="ACG8V3_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300079.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1495..4722
                     /gene="fss3"
                     /locus_tag="ACG8V3_001537"
     CDS             1495..4722
                     /gene="fss3"
                     /locus_tag="ACG8V3_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0300080.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            4836..5150
                     /locus_tag="ACG8V3_001538"
     CDS             4836..5150
                     /locus_tag="ACG8V3_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0300081.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5163..5537
                     /locus_tag="ACG8V3_001539"
     CDS             5163..5537
                     /locus_tag="ACG8V3_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0300082.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5547..5891
                     /locus_tag="ACG8V3_001540"
     CDS             5547..5891
                     /locus_tag="ACG8V3_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0300083.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            5962..7314
                     /locus_tag="ACG8V3_001541"
     CDS             5962..7314
                     /locus_tag="ACG8V3_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0300084.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7374..8516
                     /locus_tag="ACG8V3_001542"
     CDS             7374..8516
                     /locus_tag="ACG8V3_001542"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0300085.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8541..8795
                     /locus_tag="ACG8V3_001543"
     CDS             8541..8795
                     /locus_tag="ACG8V3_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300086.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9051..10235
                     /gene="mobT"
                     /locus_tag="ACG8V3_001544"
     CDS             9051..10235
                     /gene="mobT"
                     /locus_tag="ACG8V3_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0300087.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10232..10369
                     /locus_tag="ACG8V3_001545"
     CDS             10232..10369
                     /locus_tag="ACG8V3_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0300088.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10374..10595
                     /locus_tag="ACG8V3_001546"
     CDS             10374..10595
                     /locus_tag="ACG8V3_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300089.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10608..11108
                     /locus_tag="ACG8V3_001547"
     CDS             10608..11108
                     /locus_tag="ACG8V3_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0300090.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11205..13052
                     /locus_tag="ACG8V3_001548"
     CDS             11205..13052
                     /locus_tag="ACG8V3_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0300091.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13055..13951
                     /locus_tag="ACG8V3_001549"
     CDS             13055..13951
                     /locus_tag="ACG8V3_001549"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0300092.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14001..14390
                     /locus_tag="ACG8V3_001550"
     CDS             14001..14390
                     /locus_tag="ACG8V3_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0300093.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14377..15876
                     /locus_tag="ACG8V3_001551"
                     /pseudo
     CDS             14377..15876
                     /locus_tag="ACG8V3_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16624..18450
                     /gene="ltrA"
                     /locus_tag="ACG8V3_001552"
     CDS             16624..18450
                     /gene="ltrA"
                     /locus_tag="ACG8V3_001552"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0300094.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18563..19385
                     /locus_tag="ACG8V3_001553"
                     /pseudo
     CDS             18563..19385
                     /locus_tag="ACG8V3_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         6552 a         3169 c         4271 g         5393 t
ORIGIN      
        1 acaggttggt tattttcgga aaaagctaat atagctaaga ttgaaacatc catctggaat
       61 ttatcttttt tgctgctttg ctcataaaag tctgcatatt catcactgat attttttcta
      121 ataaagtctt ccatcataaa aatcacctca aaacgattat acgaacttac gttctcgttg
      181 taaagcgata tttgaggggc aaaaaggggg caaaaatacg tttacctttg atatatctca
      241 gattgaacat agtgtttatt atatgtcggt attgttgatt tatagctgtt ttgagggtat
      301 tagatacata atagaatata gtggttttgt ttacgtgcat tacacggaaa agctgctcgt
      361 atcaaagaaa tcagaagata aacacggaaa acttccttgg aaatctgaaa tttgtgaaaa
      421 gtccttgtat atcaagggct ttttcttttt tctctatcac agttttaagc catttttggc
      481 tgttttggac catttttcaa tcatattccc ttaaaaattg tcctagattg tccgacattg
      541 actatcagtg tcgtcaaaat agggcaaaat ttagtagtca gcttgacgac aaatataata
      601 cgaaaaatac caaaacattt accgaattta gcaacttttg aacccaagtt tctcaaaggg
      661 tcagctatta gcaaaaaaga caaaacatgt acttttctac taaaaagttg atgtgatagg
      721 cttctatcta aataactatt aggagttttt atcgtgaagc aaaatgatgg acgtatcatt
      781 tggaaaaatc aaagtgagtt aaaactgatt ttaacaatta atgaattcat cgaaaaacac
      841 ggaattacat ctagccgtca ataccagaaa aagctatcag aaaatccaaa ttctgcacca
      901 agtatgtggt tcattaacaa aaaatatgga tcatgggaga atttattgat tagtatagga
      961 cgagaaaata ctgggtatgg aaaatgggct agaatgtcag aacaagaatt attggaaata
     1021 gtagaagcct ttattaagtg cgagaagata acctctcaac gaatgtacga acaaaaatca
     1081 gtagggaaga atattccttc acttagtacc attaaaaaga tgttaggaga tatacgtcca
     1141 ctttttaaag aaaaaaatga tggatctcga tttactgatt tcgaactgct gttagaacta
     1201 aaaaatgaaa taatccgatt aaaactacag gatgatttat caatgacaaa gtttcgaaaa
     1261 ctagttcaat ccccaaaact accatcagtt gatacaatta tgaaaagaac gaacaaaaat
     1321 tgggaggaat tgatggcaga gattggattt gattatcgaa gaatcaaaat ttataagcag
     1381 agaaacaacc tatccaagac aaagaaaact aaatagtatc actctaaatc agacgttcaa
     1441 aattaaaatt tggacgtctt ttttattacc caaaaacgaa aggagattta ttttatgaaa
     1501 aagccaagat ttaagaattg gcgtttgctc gcaacactga gtctattatg ccaaacaatt
     1561 ggtggatcgc ttggcccaac gattgccttt gctgatgaaa ttactcatcc acaaacggta
     1621 actgtggaat tggatttggc tcaccagtat gctgtcgaag gtacatttag cgatggtcgt
     1681 cccatgtcag aagttaccgt tccgcactat gcagtttaca atggtgtgaa gcaagatatt
     1741 ttctgtattg agccaggggt gccgatttac aatgagttca ctcctggtta tgagaaaaat
     1801 ccattgcctg atatgtcgga gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt
     1861 acggatgtgg atacacatat tgtggcccag aagatgattt ggcaagaagt taatggctac
     1921 acgcttcact caattaaacg ttctgatggt agtgcagtaa atatcgcagc cattgagtca
     1981 aaaatcaatc aagccattgc ggattatcaa aagaaaccaa gcttccataa ctccacagtg
     2041 aaaaccgtgt taggtcaatc gactactgtg acagatacga acggcttgaa tttatcggag
     2101 tttgatgaag ttgtcgagaa tatcgcaaat attgattatc gtgtgaacgg caatcaacta
     2161 gtgattactc caaatgccaa ttcaaaagag agtggtgaat taaccctaaa gaagtcagct
     2221 aatacaggaa caccggtagc ttataaaatg gctggtcaac aaactttgat ggcgggggca
     2281 attgataagc ccaataccta cacaatcaaa attgatgtgg aaactgaggg ttctttgaag
     2341 attaaaaaag tcgataaaga aacaggaaat gttgtaccgg gaaccgtttt ccatttagac
     2401 tttggaaaaa cattacctgc aaaagacgtg accaccgata aagaaggcat tgcgacattg
     2461 gatgagattc ctcatggcgc gaaagtaacc attactgaaa aatcagtgcc agctccttat
     2521 acgattgaca ctacaccaat caccgcaacc attaaatctg gtgagactat ttctgtgact
     2581 tcaaaaaata ctcgcgaaaa aggtcaaatc attttgaata aaatgggggt ggaaacagga
     2641 actgatcttt ggaacgacaa atactcatta gccggaaata cgtttgccat tcgcaaagac
     2701 agtccaaccg gtgaaattgt ccaagaaatg accacggatg aaaaaggtca tgcggaaaca
     2761 ccaaaagaga ttgccaatgc gttggaattg ggaacctatt atgtgaccga aaccaaagct
     2821 agtaatggct ttgtgaatac ctttaaacca gtaaaagtcg aattgaacta tgtcaatcaa
     2881 accgtggctc ttgttaccag taacgtaaaa gggcaaaacc aagaaatcac tggtgaaacc
     2941 actttgacaa aagaagacaa agataccggt aatgaaactc aaggtaaagc tgagtttaaa
     3001 ggagctgaat atactctctt tactgcaaaa gatggtcaag cggtcaaatg gagtgaggct
     3061 tttaaaccag agttagtgaa gggaacgaaa gcttctgatg aaacggtgac tttgacttta
     3121 gatgaaaaga atcaagttgc tgttaaacac ttagcgatta atgagtatta ctggcaagaa
     3181 accaaagcac ctgaaggata taccttggat gaaacaaagt accctgtttc catcaaaaag
     3241 gtggatgata acgaaaaaaa tgccgtgatt acccgagatg ttacggcaaa agaacaagtc
     3301 attcgctttg gctttgattt ctttaaattt gctggatcag ctgctgggac tgccgaaact
     3361 ggcttcaatg atctgacctt taaagtatcg ccattggaag gaaccagtga gattacagat
     3421 gccgaagatg aagcaattac agcttataac gagcaactag gttttgatgg ctatggcaag
     3481 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaggctcc agaaggattt
     3541 caaaaaatta cgccattaga aatccgttct acttttaaag aaaataaagc ggattacact
     3601 aagagtgaat acatttttac gattaccgaa aaaggacaaa aacaaccaat taaaacggtg
     3661 accgttcctt acgagaaact gacgaataaa gcattttctg ttagcttgaa tcgtttgatg
     3721 ctctatgatt tacctgaaga agaagatagc ttaacttctc ttgggacttg gaaagacgga
     3781 ggcaaagaat tgacagacct tgattctacc gagctagttg ataaattgag ctataacttg
     3841 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga agcgacaaaa
     3901 gctgcccaag aaaaagatga aaaagccaaa ccagtggtaa ttgccgaaac aaccgcaacg
     3961 ttggcgaata aagagaaaac aggaacttgg aaaattttgc ataaattaac cgcagaacaa
     4021 gttttggata aaaccatcgt cttattcaac tatgtgtatg aaaacaagaa accctatgaa
     4081 gcaggtgatg atccagtagc gaaagatgct agcttgaaca atcaagccca aactgtcaat
     4141 tgtacggtgg aacgccatgt ttccatccaa acaaaagccc acctagaaga tggttcgcaa
     4201 acctttactc atggggatgt ggtggatatg ttcgatgatg tgtcgattac ccatgatgta
     4261 ctggatggct caaaagaagc ttttgaaaca attctgtatg cattacttcc ggataatacg
     4321 accaaagaaa tttggaaatc tggcaaaatt gattatgaag tgaatgacaa agaattcact
     4381 aaaaccgtac ttgcggaaaa agtggacaca agtaaatatc cagaaggtac aagttttacc
     4441 ttcaaagaaa ttaactatgg caaagacgga aacatcaatg ggaaacacaa tgaggatcta
     4501 aaagaaaaaa ctcaaacttt aacacctaaa aaagttccga caacaccaag tgcaccagaa
     4561 caaccggaaa taccaactgt tccaagtgac tctcaagaat ctagtcctac agtaaagaca
     4621 ttcccacaaa caggggagaa aaattctaat gttctgctgt ttattgggtt taccttgatc
     4681 tttgcgactg ctgggtatta tttctggaat cgccgaaact aaggtgatgt gatgaaacaa
     4741 ctaaatagga aaaaccaacg tgcgcgaccg cctcctaaaa aaagtggtcg cgctattttt
     4801 gtatccaaaa ctaaaaatga aaagaggaaa agaagatgga attgaaattt gttgtgccag
     4861 atatggccga aacctttgga aagataaggt atgccggcga aggcgaagtt ttaactgaag
     4921 gatatgggcg gaatactaca gtaattggtc gtagttatca tttgtattcc agtaagcaac
     4981 gagccgatga tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgatcaag
     5041 atcaaccact aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
     5101 gtgggtttac cgactacgtg ttgtatgtcg atgatttagt gaaagcatag gaggaaaaga
     5161 agatgagatt aacagaaggt attgttgtag attcaggact aacgtttggg aaattacgat
     5221 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacggtt agtaacgagg
     5281 taaaagaacg gacttataat ttgaaatcct ctgcgcaagg tcgaatgatc caagttagta
     5341 tcccagcaaa tgtaccctta cgcgagtttg cctatgacgc agaggtagag ttggtcaatc
     5401 caatcatgga tacggtcgcc aattatgttt tccgagaagg aactactgtc aattggttca
     5461 ttaaggccga tgatttagtc ttgaaacgac aaccagatca gggaaattct acaaaccaaa
     5521 atgaaggaaa gaaataggtg aagcgtatgg aaattaatgt tgaacatatt ttagattgtc
     5581 tcgatcaata tggaaaaggt gagctaacgg aggaacaact cactaaagca ttaacctatg
     5641 atgaaaaaat gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgccgaag
     5701 aagattttga tgtactgaat tggttagggg aagaagagtc cttctttatg gttatcgaag
     5761 taaatgaaag tctatgtcga gaagccgaaa ccgtattgga agaaattggt gtggaaatgc
     5821 cggatgccat tgaagttttt ttaaagcaac tagtggaaac gaaacaactc cctatagcag
     5881 taaatgacta gcataactgc ttgaaattgg atcaatgaaa tcctatgaag cttttttcga
     5941 ttggaggtgg aaaccataaa aatgtacaaa gggcatcgta tacgggcagg agatcagcac
     6001 ttggtttatc actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc
     6061 ttttattttc aagagcttag acaatttgat atttctaagc tatcactttc tactatagaa
     6121 atcgttcgtt ctataaaaga tttaatctat ttgctaggaa gtctcgttct ttcaggaagc
     6181 acgatgttgc tttatataca cttttttcag gatcattggc gcagtttgtg gcatcgacaa
     6241 aagctagccc ggatgattct agaaaatcat tggtacgaag taaaacaaac tcagagtgaa
     6301 ggatttttca aagacctcaa tagtagtcga accaaagaga caatcagtta cttccccaaa
     6361 atctattacc gtatgaagga tggtttactt tctattcgcg ttcaaatttc actgggaaaa
     6421 tatcaagatc agcttttaaa gctggaaaag aagttagaaa gtggtctata ttgtgaacta
     6481 gtagaaaagg aactcaaaga ctcctatgtg gagtacactt tactgtatga tataattgcc
     6541 aatcgaattg ggatagacga agtagtggca gaaagtgggg ctttgcgatt gatgaaaaat
     6601 caagtatggg cttatgattc tttacctcat atgttaatcg ctggcggcac aggtggtggg
     6661 aagacttact ttttactcac tatcatcgag gctttattaa aatctgatgc cgagcttttt
     6721 gttcttgatc ccaaaaatgc ggacttagcc gatttaggta cggtaatgcc tcatgtctat
     6781 tcccaaaagg aggaaatttc tgcttgtgtg gaagatttct atgagcgcat gatgactcgt
     6841 agcaaggcaa tgaaagaaat gcccaactac aaaacaggag agaattatgc gtttcttgga
     6901 cttccaccaa actttttaat ctttgatgaa tacgtggttt atatggaaat gttaacgacg
     6961 aaggaaagtg cggtgatttt gaataagctc aaacaaatcg tgatgttagg tcgtcaatct
     7021 ggtttctttc tgattctagc ttgtcaaaga ccggatgcga aatatttagg agacgggatt
     7081 cgcgatcaat ttaatttctg ggtggctttg ggtcgtatga gtgaactggg atattccatg
     7141 atgtttggcg aggttgataa aaaattcttt atgaagcgta tcaaaggtcg aggctatgta
     7201 gatacgggag ggagtgtgat tagtgagttt tatacgccac ttgtaccaaa aggatatgat
     7261 ttcttaaggg aaattagcga cattggtgga ttaaacgtta atacggaagg ataaaataat
     7321 agtatgtagc ctaggagtgt caagaatcct gggctttttt tacgaggtga tttatgacat
     7381 ttttagattt attcgctggg attggtggct tttgcttagg tatggaacaa gcgggtcacc
     7441 agtgtatcgg cttttgtgaa attgatgatt ttgctcgcca gagttataag gcaatccatg
     7501 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagacgag tttattcaat
     7561 ctctcggacc agtggatatc ctttgcggtg gatttccgtg ccaagctttt tcaattgcgg
     7621 gaaagcggca aggattttcc gatactcgag gtactttatt ctttgaaatc gctcggttcg
     7681 ccgctattct ccaacctaag tttttattcc ttgagaacgt ccggggatta ctcaatcacg
     7741 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgatgtgg
     7801 aatggcaagt gcttaactca aaggcctacg ttccccaaaa ccgtgagcgg atcttcctta
     7861 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga tcgtctccaa
     7921 catctgatca aaacatcaga aacttattaa atatcaaccc ttccaatcga ggaatggggg
     7981 gacaagtcta tgggtcagat gacgtagcgc ctaccttgac cggtgatgaa ggaatcaaaa
     8041 tcgcattgcc ggtagatgat ggaatttctg tagcgggtat gttactggga aattttgaac
     8101 aaggcaatcg ggtttatgag ataactggaa cagcgcctac tttgtcaacg aaacaaggcg
     8161 gaacgaagat catgcttcgg aaaaacgaaa cagaatatca agaggtgaag ccagggaaca
     8221 gtgtgaacct ggcttttcct aattccacaa gtcgtcgagg ccgtctggga aaacaaagtg
     8281 tgcatacctt attaacagga gaccaacaag cagtcgtcac ggatcagtat caaattcgaa
     8341 agttaacacc tagggagtgc tggcggcttc agggctttcc ggactgggct tttgatcgag
     8401 cttctcaagt caattcagat agtcagttgt ataagcaggc tggaaattct gtaacagttc
     8461 cggtcatttt tgatattgct agaagattaa aggaggtaaa aacagatgat ttatgatgag
     8521 ttgattgggg aatctactgg gtgattggca agattcagtc agatccggaa ttggaagaag
     8581 aactgtaccg gctcaatttt gatattcgca aaaacggggt gaaagtccct gatgatccgt
     8641 atgtaattga tgaggaaacg gatgctcgaa tggagctgaa tcaagtcatc agtgaattgg
     8701 aacgaattgc ggactttgct aaagaacctg atatccgcca atacttgttt gagataaaag
     8761 cagagttgga aattgagggt attaccgcgg agtaacggcc aaataaacca ctgataggca
     8821 ctgaccgcgg gggatttgcg aagcagaaat cctccgtggt gctgtgcctg tttatggacg
     8881 gcgttagccg gccatgatgt ctcacccccc gtatctaaca ggggggtaca aaaacacaaa
     8941 aaataagcaa gcaacgataa ggaatggctg tcctatcaag gataataagc aatttgtgta
     9001 gatgttaact gatttgaaaa agttaacacc ttaaatttgg aggtgagaac ttggcacaaa
     9061 ggaatttaga ttatcggcta ttaaaagacc ggcgtaatga atatggcgtt tcacaaaata
     9121 aattagctac gacttgtgga cttagtcgcc cgtacttaaa tcagatagaa aatggtggcg
     9181 taaccgcatc gacaaaaacc atgaggaaaa tttttgacca actggaaagt ttcaatcccg
     9241 agttaccttt aacgttactg tttgactatg taaggattcg ctttcccaca acggatgcga
     9301 ggaaaatcat tcaagagatt ctccatttga aatttgatta tatgctccac gaagattacg
     9361 ccttttactc ctatcaagaa caatatgtta tgggagatat tgtggtgatg ttgtcacatg
     9421 aagaagataa aggcgtcctt ttagaattaa agggtcgtgg ttgtcggcag tttgaaactt
     9481 ttttactcgc ccaaaagcgt agctggtatg actttttcga agattgtcta aaagccggtg
     9541 gcgtgatgaa acgcttagac ctggcaatca atgatcgagt tggcctgtta gatattcctg
     9601 atttaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc ttcaaaagct
     9661 atcgttccgg ggaactgttg aaacctgatg ataaagatgg tatgggcaat actttgtata
     9721 ttggcagtct taagagtgag gtctatttct gtttgtacga aaaagactat gagcaatata
     9781 tcaagttagg gattccttta gaccagaccg aaacgaaaaa tcgttttgaa atccgactga
     9841 aaaatgatcg agcttatcat gcaattcaag atttgttaaa aggtcgcagt attgaaagta
     9901 ccacattttc cattattaac cggtacttgc gatttgcgga taaggtggaa ggcaaaaggc
     9961 gaactaactg gcccttgaat gaacaatggg ggcgcttcat tggacggaat cagaaggaaa
    10021 ttcaattaac ttctgaaccg aagccttata ccattgaacg aacgttgaac tggcttggac
    10081 gacaagtcgc ccctacgtgg aagatggcca aggagttgga tcggttgaat cagacaacgt
    10141 acattcagga tatggtaaat aatactcgac tatcagaccg gcacaaaaag attttggagc
    10201 aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtga tggaaattgt
    10261 gaaagatatt ttattggtgt ttggtggcag tatgcttggt gttactgtga tgtgcttgat
    10321 gcatgcaagt gcagcggcgg accgagcaat ggaaaagaaa ggaaagtgat gaaatgaatt
    10381 tcggtcaaaa tttgtatcaa tggtttttaa ccaatgctca atctttagtc ttactagcga
    10441 ttgtggtcat tggtttgttt ttaggattta aacgcgagtt ttccaaactg attggcttct
    10501 tggtgattgc tttgattgcg gtaggtctgg tctttaatgc ttctggtgtg aaggatgtct
    10561 tactcaattt gtttaaccga attgtcggtg cctgaggggg aatcacaatg gaacagatac
    10621 gtgtttatat tgcgaacctt ggaaaataca atgaaggtga gttggtgggg gcatggttta
    10681 ccccacccgt cgattttgat gaagtgaaag aacggattgg attaaatgac gaatatgaag
    10741 agtacgcaat tcatgattat gaactgcctt ttgagattga tgagtataca ccaattgagg
    10801 aaattaatcg gttgtgtggt ttagcggaag agcttaaagg aacgccaatt ggtgaggtcg
    10861 cttcagagat tcaacatgcc ttcttcaatt ctttcgagga aatggtagag catgtggatg
    10921 atatcatcta ttatcccgat tgtaatgaca tgagtgatgt agcgtattac ttgattgtag
    10981 aagctggtga tttaggcgaa gtaccagaac acttgaagac gtatattgat tttgatgcgt
    11041 gtggtcgaga cttggagatt ggcgggaatt atttggttac gagtcgaggg gtttttgaac
    11101 taatgtaaat catttgttag aattgaccag aaatttctat cgtactttta cttttctatt
    11161 tgatatactg aaatagaaaa tatttgttga taggggtgtt actaatggtt ggcaataagt
    11221 ggtacaaatg tgatcttcat attcattcaa ctgcgagtca gtgctttcag gataaaaagg
    11281 ttactgctaa ggaatgggta gaagaatgtt taaacaaagg actcgattgt gttgcattaa
    11341 ctgatcacaa tacaggggaa aatattgatg aatataagaa agtggctatc gaaaatggct
    11401 tagatttttt tcctggagtg gaattaacgt gtggagaaaa tggaactcat ttactagtcc
    11461 tatttgataa aagtgcatgt acaaatgata tctctgattt cttaaccaag atgggtataa
    11521 aaagagagga ttgggccagc gctgcagctt gctcaatgaa atcagtttta gaagttattg
    11581 attttgctga gcacgagggc ttgctcgtta ttcctgcaca tatagatgag tttaatggaa
    11641 tttcttcagc aaaatttgga aatctaggaa agatatttga atcagaaaat atatatgcag
    11701 tacagacggt tcaaaaagaa ttttttgaaa atcgctcaca aatgattccc tcatctaagc
    11761 gtgacactat ctatgatagt gtgaatgcca gatatgatgg tagagttgga aaagatactt
    11821 tggaatcttg gtataaatca gttgttgagg cagaaaaaaa taatatgaca tttcttagct
    11881 tttctgataa cccacactcg aaaggaaatt caaaacatgg actttgggga ataggcacaa
    11941 gatattccta tataaaaatg aaggacgaac ctgacttggg atcacttcgc gatgctttaa
    12001 tgctaggcga aactcgagtt cattctgatt tttctaattt ctccattaat gaaaatgaag
    12061 ttttattgga aaaattaagc ttttctggga ccactcttag cacaaaagaa gttgtagtcg
    12121 aattcagcga taatttaact tcaattattg gaggaagagg aacaggcaaa tcttgtatta
    12181 caagattctt ggtctatgtc cttggaaagg aagcagaact tgatcaattt tctgaaatac
    12241 aaagtgatta tcagaacttt gcacaaattg aacataatgg ctctggaata tttttgaagg
    12301 atacaattgt gaaactgaat attttctata aggggacaaa atatcaaata ataagaacac
    12361 aggatagaca ttctatttat gaatttacac aagagaatgg tctggttgaa aaagaaacag
    12421 aacggttgag aatgatttcc gataaggtga gtatatactc tcaaaaacaa atttatgaaa
    12481 tttcaaaaaa tcaaactagt atactagaat tactagatgg atataactct gatttgattt
    12541 cagagtataa aaatgagatt gaaacatgtg taaacgaaat aagaaaatta aattgggata
    12601 ttgtttctgt gaaaaaagaa atacaagata aggcgaaggt agagcttgaa attgaagatt
    12661 taaaaaaaca agtagaaaag ctatctcata agagttataa agatgtttat gatgagtatt
    12721 caaaagagac agatatttat agggaactga aacgtgatgt ggaggcactt aaggaaattc
    12781 ccaaaaatct aactgatagt gttgacaaaa ttgactttgg caatggtata aaagttacag
    12841 acgaaattga tgaacaccgc ggagaattga ttaagaattt agtgaccaat cgggctaaga
    12901 ttcaaacaat aattaatgaa atgtcagatg aaattgatag ttatagagca gagctgaata
    12961 aatcagattg gaaagttaac tataatgagg tttcaaagag gtatcaaaga ggtatcaaaa
    13021 acttagtgaa gagttatctc aagatgaatt aaaaatgtta caaaaccttg attctttata
    13081 tcaatcttta tcaaaaactg aagagcgatt ggaagaaatt attcaacaag aaataaagct
    13141 taaagattac caagttgagg ttgactcagc aatgtcaaga atgaataaat catatcaaaa
    13201 tttctattct gagagaagta gttcctcgcg cgaaattttt tctgatatta caggaataga
    13261 ggctactatt caaccaaata gggatttcaa taattatgtt aaagagctca gagcttgtat
    13321 taataaggag gacacttttg acagtgagtt tggatctctt attgataagc tgacaaaaaa
    13381 ttccattact aatcaagata tatataatga catacttgat ataagagtta gtggggaaag
    13441 tagtattttt tcagataaaa aattaatcaa ttctataaga aaattgaatg atgcacaagt
    13501 tcttgaatta caaaccctta agccaaatga tttagtagtt atccgacttg aactaaatgg
    13561 aaaaaaagtt agtttatcga atgctagtgc tggtcaaaag acatctgcaa ttctttctat
    13621 gattttggcg tatggaaatt caccactcat attggatcag cctgaagatg atttggatag
    13681 tcaattaatt aataacttaa tcgtaagaag tatcattaca aaaaaggaaa acagacaaat
    13741 tttaattgta actcataatg caaatatccc agttaatggt gattcagaat ggttgattag
    13801 catgggagat tcaaaagaaa tatcggctga tcagtgcggg tcggtagatg aacccaatat
    13861 caagccaaga atatgtactg tgatggaagg tggcgaggat gcttttacaa atagagcgaa
    13921 acgctatggt tttaaacaaa ttgtagaata acactagagg gtgagacata ttattatgtc
    13981 cgcccttttt aggaggaaaa atgaaaaaaa taaaaagcta taccagtatc tgggcagttg
    14041 aaaaagtcat ttacgcaatc aatgattttc agttgccttt tcctgtgact tttaaccaaa
    14101 tggcttggtt cgttttatct ttactgtttg tcatcgtatt tgcccatgtg cctccactat
    14161 ctatgataga ggaggtcttt ttaaaatact taggcatacc ggtggctgtc acttggttta
    14221 tgtcgcaaaa gacgtttgat ggcaagaaac cactgggatt tttacgatcc tttatcagct
    14281 atcacttgcg atttaaggtg acttttgcag gaaagaaagt caaagaacag aagaaacgat
    14341 gggatgaacc cagtactctt gtaaggagtg tgaactatgt atccgattaa gtatattgaa
    14401 aataatctcg tctttaacca agaaggcgag tgttttgcct actatgaatt agtgccttac
    14461 aattactcct ttttgtcacc cgaacagaaa tttcaagtgc atgataactt tcgccaacta
    14521 attgcgcaaa atcgtgaagg caagatccat gcgttacaaa ttgccacaga aagtagtatt
    14581 cgcgccacac aagaacgatc aaaaaaagaa attaccggta gactcaaaga agtagcgaaa
    14641 caacgaattg atatacagac agatgcatta gtatccatga ttggcgatag tcagattgat
    14701 tatcgctttt ttattggctt taaactaatt gcgacagatg aagaagtcaa tctaaagagt
    14761 ctcaagaaat ctttcttttc tgggcttcaa gaatttgtct atggagtgaa tcatcatttg
    14821 atgggcgatt ttgtttcctt atccaatgaa gaaattcgct gctattctaa gcttgaaaaa
    14881 ttaatggaaa gcaaattggc tcgaagattt aaagtcaggc gagtcacacc gagtgacctg
    14941 acgtatttga ttgaacatat ttatggcgaa aaagggatac cgtttgaaga atacgaattt
    15001 cagctaccaa agaaaaagct aaaatcagaa acactggtga aacgttatga cttattacgt
    15061 ccaaatcgtt gtttaattga agaaaaacca aggtgcttac ggatggaaca tgagaatcat
    15121 gagtcgtacg tagcgtattt aacaattaat acaattgtgg gggagatgga atttccttcc
    15181 tcagaacttt tctattacca gcaacaacaa tttacattcc caatcgacac aagtatgaat
    15241 gtagagattg tgacgaataa aaaggcttta gctaccgttc gtaataagaa aaaagaactg
    15301 aaagatttag acaatcatgc ttaccaatct gacaatgaaa cgaattccaa tgtcttagat
    15361 gctttggatt ccgtggatga attggaaacc accttggatc agtcaaaaga atctatgtac
    15421 aaactgagtt atgtggttcg agtgagtgcc gaatcagtgg atgaactaaa gcgacgctgt
    15481 gatgaagtgc tcgattttta tgatgatacc aatgtaaaac tagtccggcc ctttggcgat
    15541 atgatggggc tacatgaaga attcttaccc ttaagtaaac gatatatgaa cgactatatt
    15601 cagtatgtta cgtctgattt tttggcgggg cttggttttg gggctactca gatgctagga
    15661 gaattagaag ggatttactt tggctacaat gtggatacag gtcgtaatgt ttacctgaaa
    15721 ccggcattgg cttcacaagg agtcaagggt tcagtaacca atgcgctggc cgccgctttc
    15781 ctgggttctc ttggtggagg taaatccttt agtaacaatc tcttagtcta ctatgctgtt
    15841 ttgttcggtg gtcaagctgt gattgtcgat ccaaaagggg gtgcgaaacg ttcctacttg
    15901 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
    15961 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
    16021 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
    16081 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
    16141 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
    16201 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
    16261 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
    16321 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
    16381 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
    16441 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
    16501 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
    16561 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
    16621 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
    16681 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
    16741 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
    16801 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
    16861 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
    16921 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
    16981 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
    17041 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
    17101 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
    17161 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
    17221 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
    17281 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
    17341 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
    17401 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
    17461 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
    17521 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
    17581 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
    17641 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
    17701 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
    17761 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
    17821 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
    17881 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
    17941 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
    18001 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
    18061 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
    18121 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
    18181 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
    18241 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
    18301 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
    18361 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
    18421 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
    18481 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
    18541 aaaaagttac ctatcgctat agcgaggagg gtggaaagaa accttgccag aaattgcgga
    18601 tgaaatcagt atcctcaact tgacgagtaa gtcagaaaat caaggtctgc ttgatcctta
    18661 tgtgattatg aagaaaaaga aagactcgga aagtttagca attgatattc tgactttttt
    18721 aacaggaatt tcaagtcgag acggggagaa gtttcctgtg ttacgtaaag ccattcgaac
    18781 ggtgactcaa agtgatcaac gggggttatt acaagttatt aacgaattgc gaaaagacgg
    18841 gtcaccaatc gcggaaaata tcgccgatca tatcgagtcc tttaccgatt atgattttgc
    18901 tcatttgctt ttctcagacg gctcgattac ccaatcgatt agtttagata aacagctaaa
    18961 tatcattcaa gtggcggatt tggttttacc ggatgcagaa acgacttttg aagaatatac
    19021 caccatggaa ttgcttagtg tttcgatgct aatcgtcatt tccacttttg ccttggactt
    19081 tatccatagt aaccgagaaa tcttcaaaat tgtcgatttg gatgaggctt ggagttttct
    19141 tcaagtggct caaggaaaaa cgttatcgaa taagcttgtt cgtgctggtc gggccatgaa
    19201 tgctggggtc tatttcgtca ctcaaaatag cgacgattta ttagatgaac gtttaaaaaa
    19261 taatatcggg ttgaagtttg cttttcgcag taccgatatt catgaaatca agaaaacctt
    19321 ggaattcttt ggtttggatc aggaggatca aagcaatcaa aaacgcctgc gagatttgga
    19381 aaacg
//
LOCUS       DAZKMB010000051        19155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000051 DAZKMB010000000
VERSION     DAZKMB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.051"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1499
                     /locus_tag="ACG8V3_001554"
     CDS             72..1499
                     /locus_tag="ACG8V3_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300095.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1591..1956)
                     /locus_tag="ACG8V3_001555"
     CDS             complement(1591..1956)
                     /locus_tag="ACG8V3_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0300096.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1953..2318)
                     /locus_tag="ACG8V3_001556"
     CDS             complement(1953..2318)
                     /locus_tag="ACG8V3_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0300097.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2510..2758)
                     /locus_tag="ACG8V3_001557"
     CDS             complement(2510..2758)
                     /locus_tag="ACG8V3_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300098.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2757..2885
                     /locus_tag="ACG8V3_001558"
     CDS             2757..2885
                     /locus_tag="ACG8V3_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0300099.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2942..4636
                     /locus_tag="ACG8V3_001559"
     CDS             2942..4636
                     /locus_tag="ACG8V3_001559"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0300100.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4736..4909
                     /locus_tag="ACG8V3_001560"
     CDS             4736..4909
                     /locus_tag="ACG8V3_001560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300101.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5287..6420
                     /locus_tag="ACG8V3_001561"
     CDS             5287..6420
                     /locus_tag="ACG8V3_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0300102.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6451..6771
                     /locus_tag="ACG8V3_001562"
     CDS             6451..6771
                     /locus_tag="ACG8V3_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0300103.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6800..7264)
                     /locus_tag="ACG8V3_001563"
     CDS             complement(6800..7264)
                     /locus_tag="ACG8V3_001563"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0300104.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7396..8649)
                     /locus_tag="ACG8V3_001564"
     CDS             complement(7396..8649)
                     /locus_tag="ACG8V3_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0300105.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8678..8995)
                     /locus_tag="ACG8V3_001565"
     CDS             complement(8678..8995)
                     /locus_tag="ACG8V3_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0300106.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9021..9341)
                     /locus_tag="ACG8V3_001566"
     CDS             complement(9021..9341)
                     /locus_tag="ACG8V3_001566"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0300107.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9805..11220)
                     /locus_tag="ACG8V3_001567"
     CDS             complement(9805..11220)
                     /locus_tag="ACG8V3_001567"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0300108.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11237..12553)
                     /locus_tag="ACG8V3_001568"
     CDS             complement(11237..12553)
                     /locus_tag="ACG8V3_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0300109.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12703..13401)
                     /locus_tag="ACG8V3_001569"
     CDS             complement(12703..13401)
                     /locus_tag="ACG8V3_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0300110.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13690..14679)
                     /locus_tag="ACG8V3_001570"
     CDS             complement(13690..14679)
                     /locus_tag="ACG8V3_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0300111.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14759..15592)
                     /gene="msrA"
                     /locus_tag="ACG8V3_001571"
     CDS             complement(14759..15592)
                     /gene="msrA"
                     /locus_tag="ACG8V3_001571"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0300112.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15767..17401)
                     /locus_tag="ACG8V3_001572"
     CDS             complement(15767..17401)
                     /locus_tag="ACG8V3_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0300113.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17388..>19155)
                     /locus_tag="ACG8V3_001573"
     CDS             complement(17388..>19155)
                     /locus_tag="ACG8V3_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300114.1"
                     /translation="NLTQNLLKKDNSSILKIELKKENNKSMKISKKAFLLVVLILLST
                     LYSVNFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTI
                     IPAVVFYGISNNLILSYVLYIWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATY
                     RTVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLL
                     CSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIH
                     SPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYV
                     GVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYS
                     WLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTE
                     AIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVN
                     GKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFA
                     PNKLKDSRYVTKKEINKKNENK"
BASE COUNT         6080 a         3748 c         3187 g         6140 t
ORIGIN      
        1 tgatttcaaa aagaaaacat gtcataatat ttcccatatt gtgcaaaaaa atacaaggcg
       61 gaggctctac tatgattgaa gaatatattg agaaaaatat tttaagacag ctgtttttat
      121 gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg
      181 tttgcaaaac cactttactc aatgatatca ataacataaa aaaagaattt gaagaacaga
      241 tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc
      301 gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt
      361 atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca
      421 gcaaagctta ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa
      481 tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt
      541 cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg
      601 atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag
      661 aaatcttatc gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa
      721 tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag
      781 catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga
      841 cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc
      901 aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct
      961 ttgaatctca cttcaatcaa gaattattcg gaaaccaacc gttcgaacgt gcgctgattc
     1021 atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttt
     1081 taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca
     1141 gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat
     1201 tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg
     1261 atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag
     1321 ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa
     1381 accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta
     1441 ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag
     1501 gggctgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac
     1561 aatctcttga tacacgtcag gctcacaacc tcacttattt tggacatcta cgggtccgat
     1621 ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa
     1681 aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc
     1741 caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct cgctgactcg
     1801 gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca
     1861 aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcgaa
     1921 atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt
     1981 tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata
     2041 agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca
     2101 acgactagta cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca
     2161 ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat
     2221 agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg
     2281 atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta
     2341 atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt
     2401 tctaatgata acggttcgct taaatttttc tttttcactc tcccattttt ctccctccat
     2461 ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca
     2521 tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag
     2581 aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat
     2641 cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt
     2701 ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga
     2761 gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc
     2821 taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa
     2881 tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac
     2941 aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt
     3001 ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga
     3061 tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa
     3121 ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg
     3181 tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat
     3241 gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg
     3301 tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga
     3361 ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa
     3421 tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt
     3481 tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta
     3541 tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac
     3601 acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg
     3661 atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca
     3721 agatgaaatc actgtgactg gtttgaaaat taccaataca ctgactcaag aagaagaata
     3781 cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc
     3841 tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag
     3901 tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc
     3961 tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt
     4021 gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc
     4081 aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctattg tcgtgcatca
     4141 tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta
     4201 tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat
     4261 gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga
     4321 aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc
     4381 ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc
     4441 gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga
     4501 acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta ttcctatgtc
     4561 ccgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa
     4621 aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     4681 aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca
     4741 agcttttgaa caccgtttac caagaggtta tgaaaaagct gccctgcatc aagtaataag
     4801 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat
     4861 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg
     4921 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
     4981 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
     5041 tattttttca acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc
     5101 aatgattacc attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga
     5161 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
     5221 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
     5281 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
     5341 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
     5401 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
     5461 ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa
     5521 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
     5581 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
     5641 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
     5701 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
     5761 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
     5821 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
     5881 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
     5941 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
     6001 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
     6061 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct
     6121 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
     6181 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
     6241 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgcaat gattgcagaa
     6301 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
     6361 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
     6421 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
     6481 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc
     6541 caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt
     6601 tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg
     6661 gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt
     6721 tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agaatttttc
     6781 aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc
     6841 cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat
     6901 gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg
     6961 ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa
     7021 gatttggtgt cagatcaggg cggttatgga agtcgttttc aatgacgccc gctcctccga
     7081 cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc ttattagcga
     7141 ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc
     7201 aattcgcgct ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa
     7261 ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga
     7321 aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac ggaaaacaat cacttgttct
     7381 ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga tcgtagctac
     7441 cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg ctagtacacc
     7501 acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta cccatggagg
     7561 cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa cactaactaa
     7621 gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga taggtaaccc
     7681 aaacatcgtt ggttcattaa tattgaagac acctggcgca atcgacaagt ttgttacaac
     7741 catatatggt ttgtttttac gtcctactaa ataaatagct aacagtaaac ctaaagttgc
     7801 acctgttcct cctaagttga caaagctgtc aaagaaaggt ttgttcacga taaacggtgc
     7861 gctatgtcca gctgcgattg cattaacgtt tgcttcaatg gctggcgcat tgattgtttg
     7921 catcaaagga tcgaccatat tcgctccgtg taaaccaaag aaccataaga atggtgtgat
     7981 aaatgcaagc agtaaggcag atggataaga gtttgcaagt cccataaatg gttcttggac
     8041 cactgtatag aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac
     8101 taaagcaaac gcagagatag tgatcattgc tggtagtaat gaagcaaatg cttttgcaac
     8161 tgccggtggc acaccatcag gcattttgat gatcaatttt tcatttccgc ttaaacgtac
     8221 aaagatttct gttgaaataa tcccaacaat aatagcaata aacaaaccgt ttgagctcat
     8281 acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg cggaaacacc
     8341 gtcttttcgg tagccgttag ctaaatgata agcaagcaag aaagcaatca agacagaaag
     8401 aatggcaaat gtaccgttcc aagcagcagc cccgaatcct ttccatgatt caccaccaaa
     8461 aattgagttc atcaattctt ggaacccggg aatcggtaag ttattgatca ttactgctaa
     8521 tgctcctaag atcattaacg gcatactcac cataaatgag tcccgaatcg caactaaatg
     8581 ccgttgtgca ccaatttttg atgctacagg tataaaatgt ttctccataa atgcaacaaa
     8641 tttatccata aatcttccct cttttcttta ctgtagatta cgcgttcaat cgacgataca
     8701 gatcaatgat ctcttttgct aaatctgtaa aagctataga tgtcatcaga tgatcttgac
     8761 tgtggaccgt caaaagcgtg acttcaacgt gatttccttg tgcttcttgt gtcagcatct
     8821 ctgtttgaga gtgatgtgct tggactaaag attcctctgc atctttgatt ttttcatctg
     8881 ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc cattgcatcg ctttttgcat
     8941 ttccgccaaa catgatcaga cccatgatgg cttctagatt tttttgatct tccatttatt
     9001 gtatcctccg ataattttta ttatttgttt attagttttt ctgcttggtc gagtactttt
     9061 tcaccattca tcatgccata gtcagccata ttgattacat ctaaaggaat gcctttaggt
     9121 gctagctttt cagcaaattg attttgcata aagcgtactt gcggtcctaa aagtaaaacg
     9181 tctacatttt tagattctag ttgattatcc gcatctgaag cagatacagc aaagatgtct
     9241 gcttccattc cttttgcttc tgctgccttt tgcatttttg tgactaggag acttgtactc
     9301 attccagctg aacatacaag catgattgtt ttcttttcca ttttaaacac gttcctttct
     9361 atacttacac ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg
     9421 atatcatcac atttaaggtc tataccaagt tgcatacaca ctaaaaaaga aagcgcacac
     9481 taaacacaca aattaatttt tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg
     9541 acacaaaact tgtcacaact tttttcaaat aaacggtatt caaaagcttg catctgcggt
     9601 aataagtcca accaagttaa aaacataaga tgttgttttt actttgctat tcttattact
     9661 tgatgcagga cagctttttc acaacctctc aataaacggt gttcaatcag ctgaccctgg
     9721 gtattagctc aaaacgctgg attcacaacc tcttttattt caaataacca gtaaatacaa
     9781 tgaacatcta caaaaaactt ctatttattt atagatttcg tcaggcaaat ccaattctct
     9841 cttatcaatc aaagtctgat accacgaagc agattttttc aatgttctgc ttcgttcatc
     9901 gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg gtgacacaca
     9961 gtcagtgaat gcccataaca tatagccttc gcaatttgcg ccttcttctg ttgctttgat
    10021 caagtaatgc aaatgctgtc cgataaattc gatacggtaa tcatcttgca cgattccttt
    10081 ctcatccata tactgttctt catattctct tcccataccg ttttctgtta tcagccaagg
    10141 gatattatga taattttctt ttagatacat ggctatatca tacatgattt tcggatagat
    10201 ttcccatccc ctggaattat tcattttctt cccaggtaaa tcaaagactt cataatatcg
    10261 ttcaggatgg aaaggtgtct cttcattcca ttgataacga ggggcgcgaa cgcgtttagg
    10321 ataatattga ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc
    10381 tgttgcagga tcgaaataaa tcgattgttt ttgacacaac tccaggaact cttctgtgta
    10441 gtatcctttt accatcggat caaaaaatac tcgattataa aacaaatcat acatataagc
    10501 agctttttgg tcttctggag aattggatcg agcatagacc atttcaggat ttaaaataca
    10561 gccaatcctt ccaccataat ttccgctgcg gtaaatctct actgctttag ccgttgctaa
    10621 aactttatga taattccaca tcatccactt ttttgtgttt tgttcatgcg gccagcgaac
    10681 agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa accagttttt
    10741 taccctatca ccgtaacgat caaaggcaat ttgagcatac ttagcataaa aatcaactac
    10801 ttttcttgaa gaccagccat catatttttc caaaagaaaa gctgggactt cataatgttc
    10861 caaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt cacttatatg
    10921 ctgagcatat tcttcatcta ctatacagtt ctcataatca gtaaaaaaac gagcccaatt
    10981 gatagaagtc cgataatgtg tcagatggat ggatttcatc agatccacat cctgctggta
    11041 ttgttccata aaattagttg ctacggctgg gccataccca ttatgccaaa caaaatgttc
    11101 atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt tccaaccttc
    11161 cgtttgccaa gcagaagatg cagcaccaag aataaaattt tttggaatag taattttcat
    11221 gatttcctcc tgttttttag atcccatcat tttccacacg atttgctact tttacaaatg
    11281 gcagatagat caaaatagaa acaataatac aaacaagttg cgtaataact gcaccgatat
    11341 ttcctgctgt tgccagataa gcattgacga tcggcggcat tggccaaggg accattacaa
    11401 ctgctttcgc cgcaaatcct aaagcggttg caaaataacc aatcgttcca gtgataagag
    11461 gcgttaagat aaacggaata gctaaaattg gattcagcat gatcggcata ccaaaaataa
    11521 ctggttcatt gatgttgaag atcccaggag caagggataa cttggtgatt tgtttcatat
    11581 cttcccgttt tccaactaac aatactgcaa tcagtaaacc gatcgtgact ccagacccgc
    11641 ccatactcat atacatatcc caaaatggca tagtaataat gttcggtact tcttttccag
    11701 cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttcc cgtatcggct
    11761 tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaata aacatcaata
    11821 gcaaaatacc cggtaaccct tgtacgacac cttctaatgg gcgttgtacg acattgtaaa
    11881 tgatatcata aaggtacatt cctgtaagtt ctttgatgat aaatcccgca gccgcgatac
    11941 ccgatacagt cagaatcgtc gggaataaag cagaaaaact agcagaaaca ttcgttggca
    12001 cggtatcagg cattctcagc tgtagtttct tctgtttcct caaccagcaa taaagttcaa
    12061 tagaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta tattgtttag
    12121 ccaatacgtt ttctactata acagtctgat tttcattcac taattcataa acagttggat
    12181 taacagataa ataagacatc actgcgatga taccagggaa tacaccatcc actttattca
    12241 gcttggctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga
    12301 catagctgat ggcagatgcg ataggcttta attcttctaa aaaacgcaag aaacttatct
    12361 gtgctaagcc attttccgag tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg
    12421 taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata taccttaaat
    12481 tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata aaactcattc
    12541 cagatcccct cattcctgct tcttctcctt gacgtcatct ctttttgtct attagtcagc
    12601 gcttacattt cgatgaacag aaactattta ctcctctttt ttaccaaata taaaacaaaa
    12661 attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag tttctttttc
    12721 tcctattatt ttctgtaatt ctctccgatt tattgccata tggacagtca ttgcatcaaa
    12781 cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt
    12841 ctcgattttc agatgctgga tatcataatt tttattaaac aaagcaattg attcattaat
    12901 gattgggata atatgcagca aggctacgtt tccgctagct tctgcaatca tcgtatgaaa
    12961 atcgatgtct aattgaacat gttcttgtgt gccttctcga aaagaagttt caatcttttg
    13021 cgttaatttt ttcatttgga cgatttgatt cttcgttgca tattccgctg ccaaacctgc
    13081 catcttaggc tccaataaat atctcagttc aaataagtct cgtatcatcc gatcctgatc
    13141 ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt
    13201 ccccgctcct tgccgaactt caaggatatt cctggaagct agagcccgca cagcttctcg
    13261 caccgtgctt cgccccacct ctaaataatc ggacaattca tattcattcg gcagtttatc
    13321 tcctacttgc aactgcattt cctttaaata tctaaggatc ttgtcagtag ttacttctac
    13381 tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgtat tttttcgtaa
    13441 ttattataac acgtttgaat catctgatga ttttttgtat acaaaattga ttaatccgtt
    13501 catagacata caaacgaaga agatatttac cataaaaaag actaggataa aattcactcc
    13561 tagccttcga tcatttttat tttgaacgac aaaaaatatg atgttcaata acagacgatc
    13621 ttcattcgat tcttgatgtc atcttttgtt ttttttaata tatggataaa aagtttgatt
    13681 acatcgtttc tattctagaa tttggtcgat tctcaattct tcttctggtg agaaatctaa
    13741 attatccatc attcgaacac tttcttgtaa ctgacttaac cgacttgctc cgaccagtac
    13801 acttgtaaca gatttttgtc gcaaattcca tgcaagggcc atttgcgcta atgattgtcc
    13861 tcgtgatacg gcgattgttt gcagtgcttt gattttttcc agagttgatc caacttgttc
    13921 ttctgaaagg aatggtattt ccttacgatg tgctctagaa tcttcaggaa taccatgtag
    13981 ataacgattt gtcagcaacc cttgagcaag cggactgaac gtgattgccc ctagcccttc
    14041 ttcttctaat actggagata gatcgtcttc tagtagtgca cgagaaaaca tattgtagcg
    14101 catctgatga ataatgaacg gtgcttcctt tctttttaaa atttcagtca ttttttttgt
    14161 atcttctcca ttatagttgg atataccaat atataatgct tttccttgtc ttacaagttg
    14221 catcaaagct tctgctgttt cttccaaagg tgtatcaggg tctgggcgat gatgatagaa
    14281 gatgtcaaca taatctagcc ccattctttg gagactttgg tcacaacttg aaatgatgga
    14341 tttacgagag ccccattccc catatggccc ttcccacata tgataacctg ctttgctaga
    14401 aataatcagt tcatcacggt aggcttgaaa atcttctcta aaaatacgcc caaagttttt
    14461 ctctgcagct ccagcagggc ctccataatt attcgcaagg tcgaaatggg taattcccat
    14521 gtcgaatgcg cctcttaaaa tctctctttg attatggata ggatcataat caccaaaatt
    14581 ttgccataat ccgagagata aaatcggtaa tttcaaacct gaatgaccgg atttacgata
    14641 ttccattttt tcataacgtg cactatctgc aatatacaat ttttctcctc ctcatcaaat
    14701 ctttttaaaa cgctttcttt tttctatttt tagtttacta cattttaaag aaaaggagtt
    14761 aatcaaaatg attttttcgt atattttttt gccatttgtt ttttactcgt tgatagatca
    14821 gaaattgatt acgtgcttgc tggatttttt tataacgttt cggttgtttt ttgtaaaatt
    14881 gctgatgata gttttctgct ggccaaaacg taccggctgg ttcgattttt gtgacgattg
    14941 gttggtggaa agttcctgaa ctgtctagtt tctgttttga ttgttccgct aattcttttt
    15001 gtcgttcgac ttgataaaaa atcactggac gatattgctt ccctctatct tgaaattgtc
    15061 caaatgtatc tgtgggatca gtaatttgcc aatagattgt cagcaactcg ctataactta
    15121 tttctcttgt gtcataaatg atctccacag cttccacatg acctgtgtaa ccaccactta
    15181 cttgatcata atttggtttt tcaacatgtc cgcccgtgta tcctgacaaa acagatagga
    15241 tccctttttt cgattcaaac ggctcgacca tacaccagaa acatccccct gcaaatactg
    15301 cacgttcttg atagtcagca tcttgcatgg catgcttgga aaagtcatat ttttgctcct
    15361 tatcaaatcc ttccgtaatc aataaataaa agtccattac atcaggcgtc agattcattc
    15421 ggatagctaa tggacgtagt gttgcttcta gttttgataa ctgttcttta acggatatat
    15481 tttctcttat gtcattcttt gtctgaatca atacttttcg ttcccaatct cgtgtattcg
    15541 gattcaaaat cagattataa agtgtttgaa tgaatatttg ttcctcatcc atagattctc
    15601 ctcctttccg aagaagacaa gctagtgact ttagctgtaa tttatcataa agtgaggaaa
    15661 taagcgatta cttcatcccc ctacagattt ttcgtataaa aaagagagaa tccttatcga
    15721 cagattctcc tttttaatca atttatttta taaatgaaaa gttaatttac cttttatgga
    15781 aaactatttt aaaaaataaa cataaatatg cagttaatgt aacaagataa gaaataatag
    15841 tcgttactgt ataacttgtg cttactttaa tatcagcaac tcctttgact ccgcttattt
    15901 gtaccaatcc attagcattc acctgctcct ttacaggttg atgatttatt tctacattcg
    15961 ttcctaaata agcatataca ggaagtgtga ttacttggct cttctcatta gttactctat
    16021 atatgataga actaccagtt gttttataag gaaggtttac ccattgactg ccaatctttg
    16081 cttcgtgttt tattatatgc tccttattct ccaatgaagc gataggagaa taatcatata
    16141 aaaaataatc cattccttta gtggtatacg cctctactgt ttctttgttt acaacaatat
    16201 gtcccggctg agaaattatt ttttgattga aatttttttt actagaaaca gttaaagtga
    16261 ttaataacaa aacagtcata aataaataaa atagttctct tgtcttattg tatcttctat
    16321 ttttgaaaaa tatcatcgtt aatatcagag aagagaatat tatttgcaag cttaagatcc
    16381 gccaaggaaa ttgtaacaat ttggctggcg tattttgcag aagataccat ggcaccaaac
    16441 tagttgtagc tagccaagtc aaacacgtac cataataaat ctttttttct atgtttgtta
    16501 attttttaaa taaaatcagg ggaatacata aagataacag accaactagt cccattgagt
    16561 aagtagttaa gtcgttgttc atagcttcag tcagactatc gaacacatta gatgcatgag
    16621 catataaatt tgtcacgcta ggtttgttga ttttttggta gagcatttgt tcaaacatcg
    16681 gataccaaaa agcactcgtc agaaccaatg tcattcctgc cgcactaaat aattttttaa
    16741 atatagcaat aaaatctgca ttcttgaaac ctttagaaac caaacgaata aaaataaaaa
    16801 tgataattaa tgctgtactc attaatgttg atagtacatg gctgtacact agcaaactca
    16861 tactcaatgc tagctttaca gacggatact tttcttcttt tccagagatg atttgataag
    16921 cataaaataa tacagggata acaagtgtta ttgcgatagc ttcggcaatt gcggaacgat
    16981 agtagatgtc tactgtccga taagcagaga aaatatagag acaactaaat aaaaatgccg
    17041 cttttttttg ttttaaaaac cgctcgccat aatgataagc tacgagtgtt gtacacaagt
    17101 tcaataacca aacgtaaata atatagctta caatcaaatt ttttgtcatc caatataaca
    17161 ttacagctgg aaaaaaagtc agaaagggat aaaaataatt tacaccatat ccagtataat
    17221 tgaatgtctc ataattaatc ggactggtaa aaatagtcga taaccccttg attcgagata
    17281 agtgaaaaga aagatctcca gtggtataaa tactctgtgt atttttgaaa aagatgaaag
    17341 aatatatact actaactata attaatccac tcaatattac tactttttta tttattttca
    17401 ttcttcttgt ttatctcctt tttagtcaca tatcgagaat ctttcaactt attaggtgca
    17461 aatcgtttaa aacttgaata caccactaaa caataagtca gaatactgat ccataaagcc
    17521 atgagagcag ttggagtata ttttgttgta atttctatct tattttctcc agcatttaca
    17581 tccacattca ctaagccatg ttttttataa gagtcattta cgactttacc gtttactttt
    17641 acctgtgtac ctaaataagc atatactggt actgtcactt tttgcttttt gttgcttgcc
    17701 actgtgtaag taatgttatt atctgcagct ttatacttcc ctttttccca cgattctcct
    17761 acttttactt catgtttttc taagtgaggc ctttctttta tcgcttctgt tggggcgtaa
    17821 tcgtataatc ctcccatatc tgatgttgta taacgtggga gtgtttcttc attgataaca
    17881 atatgaccgt gttttgattg gataactttt gcgtaatttt cttttgctgc tatagacgtg
    17941 acgagtagaa acaccacagt agcgccaatg attaaccatg aatatttttt attcatagtc
    18001 tttttcataa aaacaagcgt tatgatcatt gaagaaaaaa agatttggat acctaagata
    18061 cgccaaggaa actggagtaa gcttatcggt gttttttgaa acaaaaacca tggaaataaa
    18121 ttagttacgc atacccatgt agaaactacg ccaacataga tgattttttc ttttttctcc
    18181 atttctttca gtatgaataa aggaaaaatc aacgcaacca atcctaataa ccccatagtg
    18241 tatgttgtaa tatcgttatt taacgcttga atgatactat ctgaaataga aagtgcttct
    18301 agctgcaaat ctgttttgaa gggactgtgt atttcttgat aattcatctg ctgcaacata
    18361 ggataccaaa aatagctagt taatgcacaa gtcaaaaaaa cagcctttac taaattcacg
    18421 accacaaatt ttatatcgtt ttttatttgc ttgcttaaaa acaaacgaac tataatcata
    18481 caagcaatca atgcactaca caatagtgta gttaagacat gagtatacac aagtagactt
    18541 atactgacag ataaagcgat gacttttttt tctctattga atatcaatcc ataagcataa
    18601 tataaaacag ctgggataat cgtaagagcg attgcttctg caattgcgga tcgataatag
    18661 atatcaactg ttcgataagt tgcaaaagta tataaacaac taaataaaaa agcagcatct
    18721 atctttttaa aaaacttttc tccataataa aaactaatca gaatcgtaca aatatttaat
    18781 aaccaaatat atagaacata acttaatatt aagttatttg aaattccata aaaaacgact
    18841 gcaggaatta ttgtaagaaa aggataaaaa tagttcagtc catctccata attgttaaat
    18901 gttgtatagt tgattggccc ttcaaaaata gaagataacc ctttgattcg tgaaagatga
    18961 aaagataaat cacctgttgt ataaatttct tgagcatttc tcataaaatt tactgaatat
    19021 aaagtactaa gtaagataag aactactaaa agaaatgctt ttttacttat tttcatagat
    19081 ttgttgttct ccttttttaa ttctattttc agtatactag aattgtcttt ttttaataag
    19141 ttttgcgtta aattt
//
LOCUS       DAZKMB010000052        18523 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000052 DAZKMB010000000
VERSION     DAZKMB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.052"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..516
                     /locus_tag="ACG8V3_001574"
     CDS             172..516
                     /locus_tag="ACG8V3_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0300115.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="ACG8V3_001575"
                     /pseudo
     CDS             517..936
                     /locus_tag="ACG8V3_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="ACG8V3_001576"
     CDS             complement(1028..1378)
                     /locus_tag="ACG8V3_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300116.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="ACG8V3_001577"
     CDS             complement(1344..2675)
                     /locus_tag="ACG8V3_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0300117.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="ACG8V3_001578"
     CDS             2931..3497
                     /locus_tag="ACG8V3_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0300118.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="ACG8V3_001579"
     tRNA            3642..3729
                     /locus_tag="ACG8V3_001579"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="ACG8V3_001580"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="ACG8V3_001580"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0300119.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="ACG8V3_001581"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="ACG8V3_001581"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0300120.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="ACG8V3_001582"
     CDS             complement(7452..7898)
                     /locus_tag="ACG8V3_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0300121.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="ACG8V3_001583"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="ACG8V3_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0300122.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="ACG8V3_001584"
     CDS             8363..8881
                     /locus_tag="ACG8V3_001584"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0300123.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="ACG8V3_001585"
     CDS             complement(8958..9914)
                     /locus_tag="ACG8V3_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0300124.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="ACG8V3_001586"
     CDS             10080..10886
                     /locus_tag="ACG8V3_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300125.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="ACG8V3_001587"
     CDS             10883..11872
                     /locus_tag="ACG8V3_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0300126.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="ACG8V3_001588"
     CDS             11869..12873
                     /locus_tag="ACG8V3_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0300127.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="ACG8V3_001589"
     CDS             13005..13547
                     /locus_tag="ACG8V3_001589"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0300128.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="ACG8V3_001590"
     CDS             13567..14265
                     /locus_tag="ACG8V3_001590"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0300129.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="ACG8V3_001591"
     CDS             14286..14498
                     /locus_tag="ACG8V3_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0300130.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="ACG8V3_001592"
     CDS             14498..15460
                     /locus_tag="ACG8V3_001592"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0300131.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="ACG8V3_001593"
     CDS             15478..15873
                     /locus_tag="ACG8V3_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0300132.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="ACG8V3_001594"
     CDS             16036..16401
                     /locus_tag="ACG8V3_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0300133.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="ACG8V3_001595"
     CDS             16398..18209
                     /locus_tag="ACG8V3_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0300134.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18438)
                     /locus_tag="ACG8V3_001596"
     CDS             complement(18271..18438)
                     /locus_tag="ACG8V3_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0300135.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         5955 a         3131 c         3821 g         5616 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga aaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaaaga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggaggggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgtat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaatg
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttcattg aatccacctc atttattatt tgttatatct attatggaac
    18481 aaaagagaaa aaaaggctag ttttacgttt gtttgtaaac ctt
//
LOCUS       DAZKMB010000053        18137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000053 DAZKMB010000000
VERSION     DAZKMB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.053"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG8V3_001597"
     CDS             complement(164..388)
                     /locus_tag="ACG8V3_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0300136.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG8V3_001598"
     CDS             complement(586..2166)
                     /locus_tag="ACG8V3_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0300137.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG8V3_001599"
     CDS             complement(2567..3943)
                     /locus_tag="ACG8V3_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0300138.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG8V3_001600"
     CDS             complement(4071..5159)
                     /locus_tag="ACG8V3_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0300139.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG8V3_001601"
     CDS             5331..5765
                     /locus_tag="ACG8V3_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0300140.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG8V3_001602"
     CDS             complement(5843..6376)
                     /locus_tag="ACG8V3_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0300141.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8V3_001603"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8V3_001603"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0300142.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8V3_001604"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8V3_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0300143.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8V3_001605"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8V3_001605"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0300144.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG8V3_001606"
     CDS             complement(10101..10679)
                     /locus_tag="ACG8V3_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0300145.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG8V3_001607"
     CDS             11001..11471
                     /locus_tag="ACG8V3_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0300146.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG8V3_001608"
     CDS             complement(11579..12805)
                     /locus_tag="ACG8V3_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0300147.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACG8V3_001609"
     CDS             complement(13559..13759)
                     /locus_tag="ACG8V3_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0300148.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACG8V3_001610"
     CDS             complement(13893..14501)
                     /locus_tag="ACG8V3_001610"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0300149.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACG8V3_001611"
     CDS             complement(14586..15056)
                     /locus_tag="ACG8V3_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0300150.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACG8V3_001612"
     CDS             complement(15104..16018)
                     /locus_tag="ACG8V3_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0300151.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACG8V3_001613"
     CDS             16164..16967
                     /locus_tag="ACG8V3_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0300152.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACG8V3_001614"
     CDS             17145..18092
                     /locus_tag="ACG8V3_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0300153.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5455 a         3633 c         2970 g         6079 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaa
//
LOCUS       DAZKMB010000054        17593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000054 DAZKMB010000000
VERSION     DAZKMB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.054"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..808
                     /locus_tag="ACG8V3_001615"
     CDS             <1..808
                     /locus_tag="ACG8V3_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0300154.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1161..2009)
                     /locus_tag="ACG8V3_001616"
                     /pseudo
     CDS             complement(1161..2009)
                     /locus_tag="ACG8V3_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2162..3274
                     /locus_tag="ACG8V3_001617"
     CDS             2162..3274
                     /locus_tag="ACG8V3_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0300155.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="ACG8V3_001618"
     CDS             complement(3352..3645)
                     /locus_tag="ACG8V3_001618"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0300156.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="ACG8V3_001619"
     CDS             3813..4127
                     /locus_tag="ACG8V3_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0300157.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="ACG8V3_001620"
     CDS             4148..6127
                     /locus_tag="ACG8V3_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0300158.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="ACG8V3_001621"
     CDS             6140..6466
                     /locus_tag="ACG8V3_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0300159.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="ACG8V3_001622"
     CDS             6482..7000
                     /locus_tag="ACG8V3_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300160.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="ACG8V3_001623"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="ACG8V3_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0300161.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="ACG8V3_001624"
     CDS             complement(8436..9941)
                     /locus_tag="ACG8V3_001624"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0300162.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="ACG8V3_001625"
     CDS             complement(10033..10623)
                     /locus_tag="ACG8V3_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0300163.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="ACG8V3_001626"
     CDS             10802..12448
                     /locus_tag="ACG8V3_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0300164.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="ACG8V3_001627"
     CDS             12497..13216
                     /locus_tag="ACG8V3_001627"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0300165.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="ACG8V3_001628"
     CDS             13695..14642
                     /locus_tag="ACG8V3_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0300166.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="ACG8V3_001629"
     CDS             14654..15574
                     /locus_tag="ACG8V3_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0300167.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="ACG8V3_001630"
     CDS             15615..17072
                     /locus_tag="ACG8V3_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0300168.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..>17593
                     /locus_tag="ACG8V3_001631"
     CDS             17417..>17593
                     /locus_tag="ACG8V3_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0300169.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITI"
BASE COUNT         5524 a         3015 c         3588 g         5466 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ata
//
LOCUS       DAZKMB010000055        17337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000055 DAZKMB010000000
VERSION     DAZKMB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.055"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..605)
                     /locus_tag="ACG8V3_001632"
     CDS             complement(60..605)
                     /locus_tag="ACG8V3_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0300170.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(617..1309)
                     /locus_tag="ACG8V3_001633"
     CDS             complement(617..1309)
                     /locus_tag="ACG8V3_001633"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0300171.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1328..1612)
                     /gene="macP"
                     /locus_tag="ACG8V3_001634"
     CDS             complement(1328..1612)
                     /gene="macP"
                     /locus_tag="ACG8V3_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0300172.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1625..2188)
                     /locus_tag="ACG8V3_001635"
     CDS             complement(1625..2188)
                     /locus_tag="ACG8V3_001635"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0300173.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2416..3078
                     /locus_tag="ACG8V3_001636"
     CDS             2416..3078
                     /locus_tag="ACG8V3_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0300174.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3202..4398
                     /locus_tag="ACG8V3_001637"
     CDS             3202..4398
                     /locus_tag="ACG8V3_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0300175.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4499..5875)
                     /locus_tag="ACG8V3_001638"
     CDS             complement(4499..5875)
                     /locus_tag="ACG8V3_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0300176.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5912..6778)
                     /locus_tag="ACG8V3_001639"
     CDS             complement(5912..6778)
                     /locus_tag="ACG8V3_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0300177.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6867..7892)
                     /locus_tag="ACG8V3_001640"
     CDS             complement(6867..7892)
                     /locus_tag="ACG8V3_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0300178.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7910..8584)
                     /locus_tag="ACG8V3_001641"
     CDS             complement(7910..8584)
                     /locus_tag="ACG8V3_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0300179.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8768..9661)
                     /locus_tag="ACG8V3_001642"
     CDS             complement(8768..9661)
                     /locus_tag="ACG8V3_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0300180.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9735..11432)
                     /locus_tag="ACG8V3_001643"
     CDS             complement(9735..11432)
                     /locus_tag="ACG8V3_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0300181.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11425..12738)
                     /locus_tag="ACG8V3_001644"
     CDS             complement(11425..12738)
                     /locus_tag="ACG8V3_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0300182.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12738..16679)
                     /locus_tag="ACG8V3_001645"
     CDS             complement(12738..16679)
                     /locus_tag="ACG8V3_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0300183.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16788..17246)
                     /locus_tag="ACG8V3_001646"
     CDS             complement(16788..17246)
                     /locus_tag="ACG8V3_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0300184.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4852 a         3896 c         3014 g         5575 t
ORIGIN      
        1 gttttatgct cttcttttat tattttgtgc tttttatttt atcaaatcac tttgtttttt
       61 tataaaattt ctgcgcctag tgtggactca aaatgtctca atgcccattc atgtccaacg
      121 ggatcgaaac ttgccacgga atctttgaaa atatggagtt tgtatcctag attatacgca
      181 tcgactgctg tatgcaacac acagatatct gtacatacgc cggtcaaata aatatctgtg
      241 atctggcgtt ctcgcaaacg gatgtctaga tctgttccac taaaagcgga ataatgccgt
      301 ttgtctatcc aataaacatt ttcctcttgc tcatagtctt ggtataaact ttttaaagag
      361 ccgtacaatt cccgtccacc cgttcctatc acattatgcg gaggaaataa gcggttctcc
      421 ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa atccttttga
      481 tcaataaact tctttgtatg agcagtcagc tttttttcaa ttacttgtcc tgctgtccca
      541 gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg
      601 ttcatcttag tcctccttat acgagatgtt tgacaaattc gatcaccatt tctgctgatc
      661 gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt
      721 cgctcattgc acgtacgatc aaaaatggga tattgaactg acgagctgtt tgtccaacag
      781 cggctccttc catttcgcaa gctaatgctt cagggaaatt cgttaagatt tctttgattt
      841 tatctggcga gtcgacaaat gtatcacctg taacgatcaa tccttctttt gcgttcaaat
      901 tcgttgcttt tgctgcttta accatttcac tgcgcaaata tgtgcttgct tcataatata
      961 gaggcattcc tggtaattgt cctgggttgt agccaaatcc tgttgcatct gcatcaaaat
     1021 aagctacttt gtctgatata acgatgtcac ctacttgtaa gccttcaccg attccgccag
     1081 ctgaacctgt attgatcacc atgttcacgc cgtattgttg gatcaacaga cttgtggtaa
     1141 tagatgctaa gacttttcct ataccggaac gtacgacgat cacttcgtgg ttacctaatg
     1201 aacctgaaat aaataaagcg cctgcgcgtt cccatgataa tggttcgctt aggttctctc
     1261 tcaaaatttt tacttcttct tccattgcgc cgataatacc gattttcatc tttttaattc
     1321 cttctttcta tataaatgcg attgctaaaa aaaccacaca taacattagg ataacaataa
     1381 taagagattt catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg ctgatccttg
     1441 ttttcgtgat tggtttattc tttttgcttt ctttacgata aaagctggca atcttttttt
     1501 cttcttgctg ataagctgtt tctgcttttc gctgtttttt taatgactcg cttgcttgtg
     1561 cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc ataagccgat
     1621 ccctctactt tcctttggtc aatagttccc agtattgaat cgcataaatc gtctttgcat
     1681 cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc aagagttaga tgatacaact
     1741 ccagtacttc gtcttcatct tgtgctaaag gattctcgac cttctctacc ccttgtgcat
     1801 gataaatatg aaggacttca tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc
     1861 tcagcgactt cgcacgatag ccagtttctt cttctaattc tctagctgct gtcatttcgg
     1921 gattttgtcc ttctcctgga tcgatttttc cagctggaat ctccaaaatc actttttcta
     1981 aaggttttcg aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc
     2041 cgacaccgcc aggatgaaat accagttctc tcttcgaagt tcctccatct ggcaaacgaa
     2101 cttcgtctac agcaacatca ataatttttc cgctatagat ttctttgcga ctgattgttt
     2161 tttcttcaaa ttgctcattg ttcagcatat cttctcctgc tttctttgta atcataactg
     2221 cttttttttc atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc
     2281 ttagattttc tctaaatata gaaaatcctt ctttagtccg atactctttc tagtagtccg
     2341 tatgctaaaa taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga
     2401 catggagggt ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta
     2461 tttctctttg tccgaaacaa tagtttttct tttgttctag gacttttatt gataggtgta
     2521 gttttagttt tgtggggact ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt
     2581 cctgcactga ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa
     2641 atttcatttt ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca attgcaaatg
     2701 aatatgcagc agacagccaa acttaaagca atcgatctcc gccatgatcc agtcaaagca
     2761 gcgaaagctt tattcaaaga attggtgaaa gaaccaaacc gactgcatga agcaagtcag
     2821 tttctttata cgcatctgcc gaatctagtt gatttgacaa ataagtataa tgagatcaat
     2881 gaccatgaga tcaaaaacaa acagacctat gaaaaactag aagaaagcgc gcagatcatc
     2941 gaccagctgt cagctttaat cgcacaagat tatcaaaaat tcgttgctga ggatctagat
     3001 gatatcgacg tagagatatc tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa
     3061 ccagaacaaa aggattaact cttttgttca taaaagaaga ctcggaacat tcagatgatg
     3121 tctggctgtt tcgagtactt tttctaaagt agagacattc atgtaagaat cgagaacaga
     3181 ttcaacaagg aggaattttt tatggatcat aaaccgactg aaacaaaaga tgttacacca
     3241 gttaacgata cacttgaaga tttattgaac aacccgtttt caactccaac agatacgctg
     3301 accacttcac aacaagcgga gattgatcag ctccaagagc aacagacagc tgctcgttta
     3361 attgacaaac taccggcgga acgccaagaa caagctaaac agctagcagc aaaaatcgat
     3421 gctaatgatg cacagtctgt catcagctac ggatcggctg cccaagcaaa attaagtgaa
     3481 ttctcacaat cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt aggagattcc
     3541 ttaactgaac tgatgtatcg tctccaagaa gccaatcctg acgaactgcg tgccggagaa
     3601 gggaacatct tccagcgtgt cttcgggaag gtcaaacaat cgatctatga agtgacagca
     3661 aaatatcaaa agattggtgc acaaattgac aagatttcag tgaaattaga taaagaaaaa
     3721 gacggtctgc taaaagataa tctgatgtta gaacagcttt accagaaaaa caaagactac
     3781 tttgatgcac tgaatattta tatcgctgct ggagaattga aaatggaaga acttcaaaca
     3841 acgatcattc cagaagcgat gaaaagagca gaagaaactg gcgatcagat ggatgttcag
     3901 attgccaatg attacacgca attcctagat cgtctggata aacgtacgca tgatcttcgc
     3961 ttagctagac agatcacgat ccagcaagca ccacaaatcc gtttgatcca aaacacaaat
     4021 caggccttgg ctgaaaagat ccaagcatct atcgctacag ccattccgct gtggaaaaac
     4081 caagtcgtga tcgcattgac cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa
     4141 gtctctgaaa caacaaacga cttattgaag aaaaattcag aaatgctgaa aatctcagcc
     4201 atcgaaacag cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct tcaaaccact
     4261 caaaatgatt tgatcgaaac aatccaagag acattgcgta tccaaaaaga aggaaaagaa
     4321 aaacgtcgtc atgcagaagt agagcttgga cacatggaag aagatctgaa acaaaaactg
     4381 ttggatttaa cccaatagat cacctttcgc ttgttactaa aacaaacgcc caacaacagt
     4441 ttattacaac tgttgatggg cgtttttctt atctttcttc taacttctcc attggagtct
     4501 atttctcatt catcttgctg tgtccatagc catagccaaa ataaatgatc agtccaataa
     4561 ccattacgat accgaacacg atccatgtca cagtttgcag acgaagcatc aatacaatac
     4621 aagtcaagat ggagacgatt ggcaatagcg gaacgaatgg cactttgaat tctccagctt
     4681 ttggtttgcc aaacattttt ctcaacttca gtaagcctag actaacaagc atcaaatagg
     4741 cgagcgccat gatattcgtt agttccgcta atacattcaa tgggaaaaat ccggcaaaga
     4801 atacagctgc cacaccagcg gcataagtcg cttttttcgg tgtacgatgc ttttgatcga
     4861 tttctgtcat aaacttagga agtaagccat ccttgctgac agcaaacagt aaacgagcaa
     4921 gtgaatacat catcgagatc gttaccgtca gtagcgttaa aattgcccct acagcgatca
     4981 ccgcgcccac tgttccatgt cctacatacc tcattgcaaa agccactggg tcattgactc
     5041 ctagttcagt atacggaacg attcctgtca agatcagcgt cacgatgatg taaaggacag
     5101 tagcaatcag tatcgaacca atgattcctc ttggcacatc tttttgcggg tttttgactt
     5161 cctcggctgc cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac
     5221 catctagcac accggaaaaa ccaaaaggca tgaatggctg ccagttatca ggtttaacga
     5281 aaaatattcc tacaatgata aacaacgcga tgataccaaa tttgacatat accatggcat
     5341 tattcaaacg taatgctttc ttggcttcaa gcgacacgat gtaagtgacg aatatcacta
     5401 cgagcaccgc aatcaagtca atgtatgtac cattttccgg attgtacccg ccagttaatg
     5461 cttgtggaag ttcggcccct aaacttttca aaaaaccttg catatagcca gaccatccgg
     5521 aagcaacaga ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct gtcagccagg
     5581 cagcaaattc accaaataca acatacagat aggcatatgg cccaccaatc actggcactc
     5641 gcgaagcaaa ttctgcgaaa cttagtccag acaaaatgac aacgattgca gctaagacaa
     5701 atgaaagtga cagtgctgga ccagcatttt gattggccgc aacccctgtc accacaaata
     5761 ttcccgtacc gatgatcgca ccaattccaa gcatgatcaa atcagacgtt tttagttctt
     5821 ttttcatctc actcgtatga ttgagattga tctctttctt acgaaacaaa tccattgaag
     5881 ttcctcccca ttttaggttg gaaaacagcg attaaactgt tttcatatac cctaaaagga
     5941 taccacctaa aatgactaaa aggcaaccaa caataacata aatgagctct tttttcgttt
     6001 ttctttcgcc aaggatgaaa atcccaccta aagtagagat aatgatcccc atttgtgaaa
     6061 gtgagaagcc aacagctaaa ccaagtgatt tgactgttag aagcatgcag atgtttccta
     6121 agccccataa taatccacag atcatgtttt tccacacaaa ctgatcgact ttaactttac
     6181 ggaaagcaaa gaagcttgcg cctaagatca ttccgatact ttgaggtaag ataattgaca
     6241 tcgcatccaa attcgcccag ttgacgatga tggtatatac accataacca atcgtagaga
     6301 agatcaatgc tctaaaaccc ttaccaaaat caagcatttt attatctgta tctatttttc
     6361 cttcgtcatc ttgtcgggca gtcaaatacg caccgcttac aagaagagct aaagcaataa
     6421 taccgtatac ataatctttc gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg
     6481 tattcaaaat taattgaaaa ccagtagaca atggtagccc tacagaaaca cccatgtact
     6541 tcattccatg gaactgtcca ttttgtccaa cactccaagc taagccggat aaaaatccga
     6601 tcaagaaaat ccaaggggtg attacaggac taacaacgaa aaagacaact aaagaaaaca
     6661 gaaatgcacc gatcgtcatt cctagtgttt gttgattggc atcgccacca attttaccac
     6721 ttactaaacc aatactaccc catgccaaca tagggactaa tgctattaaa atttccataa
     6781 ccaatctcct tttatttcac atgataagaa acttatcagc gaaaacgtta acaacacaag
     6841 cacttttttt cgtttctaac gattttctaa cgtttttcat tataaaaatt taagattggg
     6901 aaaaataaag tcattgttgt cccttttcct tcagttgatt cagccgtcaa atggatacct
     6961 aatttttcgg ccagattttt tgctaaataa agtcctaatc ctgttgaatg tgttttgcta
     7021 agtcgaccat ttctcccagt aaatccctta tcaaagatcc gtttcaagtc ttccgctggg
     7081 ataccgatcc ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt ttctatgctc
     7141 acaatgatgt ttccgtgatc cggcgtatat ttgatggcat tactaagcaa ttggttgaag
     7201 ataaaagcga tccatttagc atcggtcaaa acagtatgat cctcaccaac gatctcgtag
     7261 cgaatatttt tttggataaa ataatttgcc tgactgcgta tcacaggctg aatgattcct
     7321 tttagtccat attcttggat gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac
     7381 aaaacttgtt ctacgtattc atcgattttt gataattcat tttctagtaa tgtatacttt
     7441 tgatcgtcaa tatcgaactc gatcgaacga actagcaatt tagaagcgga aagtggcact
     7501 ttgatttcat ggacccatga gtcaatataa tctttttggt cttgttgatt actgattgct
     7561 gattgcatta cttcttggtg ttcacggatc agctgattga tatattcttc taccagtttt
     7621 tcttcttctg tcctcccacc actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg
     7681 gctaattttc cccaccatct tctcttgctc aagtaaccac ccagcagata aaaaatcaga
     7741 aagacgccct caatcaaagc taaataagca attgtgctca aatcaacttt gatgtttggc
     7801 gccagccaca tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc
     7861 cactgatctt tcaaatattt aaaaaaattc atcttaaccg ctcatccttt cagggaacga
     7921 tatatccctg tcctactttt gtttcgatat atccttggat acctgcctgt tcgatttttc
     7981 ggcgtaaccg attgatgttg actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc
     8041 acagtgctcg cagcaatttt tcccgactta agatctttcc attgtgcttc atcaaaataa
     8101 ataatagacg atattcattt ttgctcaaat caatgatctc tccgttgatt tccatacttc
     8161 cgttgtcaac attcaacgta atgccattgt ggctcatcat ttcactagat aattctgtgt
     8221 agttgtaaga acggcgaagt aatgcattga tttttgcaat cagtacatcg atttggaaat
     8281 gttttgtcac aaaatcatct gcccccatgt tcatagccat gatcatatcc atattcgtat
     8341 tcctgctgga gataaaaatg atcggtactt tcgatacttc acgtattttt tgacaccaat
     8401 aatagccatc caaaacggga agattaatat ctagtaatac aagctgtggt tcttctcttt
     8461 caaagtctga gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct
     8521 caagttcctc catgatcaat tgtcgaatga tctcttcgtc ttcgactacc atgatttttg
     8581 ccataaaaaa tccctccatc tttcttcttg ctactacaat atcaaagaag cccttgtgaa
     8641 acaagttggc ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg
     8701 cgatttaatc gcgtttgtct cttccaagca gcttaaattt attatgttat tcttaataat
     8761 tggtctatta tctgttattt ttctttcgtt gtttttgtct tttaaatttt cgataacacc
     8821 aataaccgaa aagcgttagc actagcaata tacctagaag gagtataagt aatcgataaa
     8881 gatggtattt ttttgtctgt tctatctttg aagagacttc ttctgtaaag ggaactcgtc
     8941 ttcctgtcac tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga
     9001 ctagatcctg cttttctcta attgctaatg tatttgtttt tgttggaagg acaacaattt
     9061 tctcaacgac ttcataagcg agcatccgac catacacttc aagatagaat cggtcatctt
     9121 gctccatttc ttcaagatcc gtaaagagtt ttttctcagc aataccgcta tgggcggtaa
     9181 tcacggaatg tgtactctct cctccagttg gaaaagaggt gccttgcagg actgtcgctc
     9241 ctctatctaa caaaagatca ttcgtttcat caaataaagg caagctaaca ttgattttcg
     9301 gaatatagat ggctccgatc atatgttgtt cgtaatattc ttttcctgga ttttggacat
     9361 ttctaaccgc ttgctcaaaa ggatctttga cttgtcccat tcctgggatt actgatgtat
     9421 gagcaagcgt ctgatttttc ttttcatgca ctgctaattg ttgctcatgt ttttcttctt
     9481 tatcgcgttc aagctgcttt tgataattct cgatccttct ttgatccaaa taattgctga
     9541 gagcatctgc taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca
     9601 taaataattg taataaaaga ctgatttggg tttgaggtct attacctatt ttctttttca
     9661 ttttcttcac cttcttattt aaaaaaactg cagcaacctt tataaaaccg attgctgcag
     9721 cttatttttg ttgcttatac ttcagacttc attttagatt tacggtacca gctgtacgct
     9781 ccaatcatca aacttattcc gattagtaag aatgccaaga gcccgttccc accagtagca
     9841 ggaagtgacc cgcgtttcat attcggtact ctttctggcc ctggattttc tactcctacg
     9901 atctgaattt cttcattata gcttccataa gagatgataa attcagtaaa ggctgcctca
     9961 cctgttggaa gtacataacc ttctggcgcc atggtctctt tcatttgata ggttccataa
    10021 tctaaccctt gaacctcaaa gtatccattt ccatctgcga caaattctgt tgcttgtccg
    10081 cttgttgccc actcaattct atctgctcct gattcataaa cagctaaagg cgactcccct
    10141 ttataaaata ttgcgtattc agaacggtct tcattccaca attcgaatct tgcatcttta
    10201 agcaattcac tgcttgatgc atcatatttt tcaaactttc ttccacctgt tgtgactttc
    10261 tctggcggct ctactccttc ttcataatca aattcatcat ttcgtccgat ttcaataacc
    10321 gctgtgttgt tatggaaatc atccgcttct agttctgtgg aaaccttcaa attatacgtg
    10381 atcgttaaat ctcctccacg tgcatctgct aatctaccta aagtatctac tttggctact
    10441 ccattttctt ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc tactatataa
    10501 tcttgtcctt ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg atcataataa
    10561 gttagaccaa ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc gcttccgttt
    10621 tcaaggacag aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga agccgttttt
    10681 cctctttcta gatcaatata agaaatcgtt tcttgttctc cagcttcatt aataatcgtt
    10741 acttgtctaa ggtcgctttc ctctacattc atctgtttat ctgcttctct gatttcgttt
    10801 ttcggataca aatgcacatc tgtattgtat gtgtctttcc atgtagaacc atcccacacg
    10861 ccatccacca tatcaggcat catcactggc atcataacaa ccattggaga cgcgattctg
    10921 gaaatatgag ctggcgaata agtttctaag aaaagataga ctttatctct agaagaaggt
    10981 gcttcttccg tcacttggac ggtagagaat accgcttctc cagcttcatt cgttgtttgt
    11041 ttgtcaatac ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg tttttgaatc
    11101 cattcaatcg cttcggcaga attaagaccg tcttctcgtt ccggtgttaa aggattatct
    11161 cgaatcaaat cgtaatatac ttccgttacg tcaaacaaat caaaatcgac tcctggaata
    11221 ttttcttcac caaaatccgt agaaaccaat ccgctattcg gtcgttcttc cggaacagat
    11281 gagaaacctt ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg
    11341 gctttgacta gttttccgtt cccgaaaatc cctattcccg cagtaacgag taacagtaca
    11401 ctcattcccg tgacaatcat ttttttattc atttttttct ccccgttctt tttttattaa
    11461 taaaacagca cctaacaata aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt
    11521 cgtttgcggg aatacggacc attttcctga ttgacctgga ttgcttggac cactcggctc
    11581 tttcggtttg tccgttgttt cttttacggt ctcgttataa acacgtggtg ttgcggattc
    11641 ataggcagag actggaactt tcccggattc taattcagcc atctcctgtc cgttaactac
    11701 tacatattgt ggttggcctt ttgcatccac ccaaaattca gggatgatga cctcgattct
    11761 cttacttgct tcatccatct cgtaaccttc cacacctttt aattcttcaa aataataggt
    11821 gccagaaggc aagaaacgtt gtcctgtcgt aacaaggccc tctttatccg aaatgaattt
    11881 gcttacattt ttgtcattca aaggatcatt gtctgctggt ttgatccatt gatttttcaa
    11941 gtcattagca ggagccatat ctaaataata tttcccccct tctatcatct gatatagagc
    12001 aaaaacagca ccttctaatg gttttccttt ttcagttgtt cctctttgac gtccatactt
    12061 gaagaagtat ggatctctta gataaccgac atttttagga tagatatgaa tctcttgcag
    12121 ttcctcggtt gattctgtcg ggtgataaat tggcaaaata gcagcaattg ggatagctgt
    12181 ctgatcgata tcaacatcca atccaacttc tggatcaatc gattcttcga aaatcaaata
    12241 aactgcatcc cgatcttggt ttttcttagg tgctttgata cgtgcaatcc cgtctcctaa
    12301 tccgccaacc gtatctttcc cagttgttac cgaagtccct atttggacaa gcctttcttc
    12361 tgtgattagc tgacggatat tgtttctgtt cgtttcagcg atttgctgga cgaattcttt
    12421 aggagtcatg ttttcagcag cttttttttc atgataataa tcggtcatgt cgtaaatggc
    12481 aaatgtcgct ccatttagtg gcaccgtctg tgaaatgata tctgctgttt cccctgcttc
    12541 ctcagagata gccaagccat cattttcatg gtattcaaat tgatcgttat aatcgatatc
    12601 tcgaatcatc cgtttatgca acacgaattc cacgacatct tcccctgatt cttctgccga
    12661 catcgcatgt ggaagaagga ctgctgcaag cggtagtaaa acaagtaatt gaagcatcaa
    12721 acacaacagc tttttcatta ccttgctccc tcctttttag attgtctgac aaacaaacca
    12781 taggctgctc ctgcaagcgc catgaagaat gcagcaagca ggatatagaa taatcgtccg
    12841 gatcctcctg tagcaggcaa cggattttca ggatcgttcc cgacactaag ctgtaatctg
    12901 attcgatttg tctccaaaag ttcatattct aactcttgct gtagatcagg cgtctctgct
    12961 tcttcgtttg gataatacga aatctcaact tttagtgtac ccgcttcttt tcctggaact
    13021 acccgatcat tttctatgtc aggttcttct cgataactaa tttctaaaat aaagtaacct
    13081 ggaagttttc tatagctgtc aggtgacttc acttccttga tccgatagac cccttccaac
    13141 aagtctgaag cattgaatag ggaagaatcg tttggatctg gtgccaagcc tccctcgatg
    13201 atttcatact ctcctgcact attttgtttc tcaattgaaa attctgcgcc ttcaagacgt
    13261 cggctattca tactgtcaat tttcaaaagt tccaaatcga ttggcgctag ttcatcttct
    13321 aaggttactt gtagtacacc atcgatgatt tcatagcctt ccggcaaggg agcagtttcg
    13381 tcttcgatga ccgtatcttc aatgataaac ttaccttctt cattcagtgc aaactggaac
    13441 acttgatcag atagccgata cccatttggt gcgacagatt ccgagacctc atattcacca
    13501 atagacagat gcagaggttg gtcgtaattt aaatcgacat acttttctac tcctacggca
    13561 cgagaataga agtatccgat cccaggcaaa cgtttcgatt cttctggttc taacggatta
    13621 ccagtgtatg ctcgatacag cgggaaggaa gaatccccca ctttcctgat atcgaaacct
    13681 gcaccatcta atgtatggcc ttcatggtct aattttctaa ctcggatctc ccgataatca
    13741 aacgtattgt aagcagtgaa tgctacccat aaacgatcct cttcatctgt tgtatacgtg
    13801 agatattgcc aattttcttc acctgtttgc ttgagttgga tagtccctat agcattatcg
    13861 taaagtcgca agatccatgt gtcattttca tcaggttctt tgaaaccttc tgggttctgg
    13921 ctctcaatga ttttgtattc tgcatagttg gtagtttctt cactatcctt ataaacaaat
    13981 gaatctggct ctccgattgg atgaataact ttctcaccat ccacgattac atatttcccg
    14041 tcttcatcta ctgaatagaa cgtgagtctc cctttctctt cctctacttc gttcacacca
    14101 gaaagccctt tcgctactgg atatccgatt tccagtccgt taccatctgt ttcataaagt
    14161 tcaaactctg cgccattgat tggcatatta ctgccttcaa ttagtttatt gatattcatg
    14221 tggattggct tgaattcatt tcgcatttcc agtttgacat acaactcatc atactctgtg
    14281 ccttctatgt taggctcttg tacaacagaa agcttgtgat attctccttc ataataggaa
    14341 ttcggatctt taccttccgg tccgccgact ctcgaaccag atgtatattc atctacaagc
    14401 tcgtagtgac ctaacatctc atcttcgttg gctgcatatt ttttgatttg gaattgatac
    14461 gtctcatcta ctaactgata acgtggtggt ccagcgatct ctctgatcca aaatatgtcg
    14521 ctgactgctc cagtttcatc tcctaaaatc ggcatcttcg tttcaaagat cgctattccg
    14581 tccgtggtag ttttacttgt ttcgattggt tcggcttccc cactctctgg attttggtca
    14641 tataatgcaa attcgactcc agccaacggc tcgccatttt gacccttctt gctgacttgg
    14701 aaattacggc ctttatgttc gtaacgaatg gaagaagatg ggaaatacat ccgtccagta
    14761 gtgatatcat tctcataatc ctccaggttc tgaatatagg ccatattgtt cactgtgcaa
    14821 aaatttccgt cttgagctcc cgcatcaagt tttacttggt acttgatcct gaccatttcc
    14881 ccttcaccta aagaaatcgg agaaaattta taagcttggt tttcccgtcc gacatacgtt
    14941 gtcggagtcg ccactacttc tggtggggct tgtccatgac gttcttcatc ccatatttct
    15001 ggctcttggt acttcatgcc gttttctcct ggatctagat ggtaaaactg aatatctgcc
    15061 gatctgccag ctccaaatgg tgagacatta aaaccagttt catcataaag tgtggcttgc
    15121 tggatcgttc tcttgaaggt agaagagata tcctccaagg ctgtaacaat gtcattcttg
    15181 ttgttcgcat tgtaatatcg cggtgttacg ccatcttctt ctaaatcagc aatatttttc
    15241 aatacatttc gtccaatagc atctagtcgt tgtctcccgc taacgtttcc atcgattcct
    15301 aaaccaatgg agaagatact tggcgggttc tcttgatact tgtttttcag ccaatgatga
    15361 taagcaactg tgttgatcgc agatgacttt tcttgcgaag taggactgcc attcgtattg
    15421 ttattagaag aaccatcacc aacccagtga gtcgctttat tatctccttc ttttacctct
    15481 ccataacgat agtgtacatt tacttcatct tctggacggt taaagtcaga tgcataggta
    15541 actggatgag taaaccctgc attagaagta ttcccagaaa actcctcaaa atttctgaac
    15601 aaaacgtcat tatcttcagc aatcttatct tccatcacag tccagttgct ccaagctcca
    15661 ttagtgctcg tacgataact tgactgaatc ggtgcataac tgaaggtcgg tgtcccatct
    15721 cccacaacga ttaggatttt ttctggattt ctattttctc cgccattgtc tttatacaat
    15781 gtttcatagc catttttaag ccctagtgta agcggagttc ctccgattgg tgtacgattt
    15841 aatgctgtat tctcacttcc cgtcaaacga ttgatatcat ttgtcaatgg aaagatgtcc
    15901 gaactgattt gctcatggtt attgatagct gtactattat ggtaaaaatt caccataccg
    15961 attcgaatat ccagactcgg gtcagagagc agatctttac tcatttcgtt tactgcctca
    16021 atcaacgcag catttttagt ttgactattg gtcccagcgg ttaattcact catactcgct
    16081 gacttatcta gaacaaaaac gatatcgatt ggactgattt cctttttctc gcccccgatg
    16141 acatctagat agacattgta aagggttgtt gcatcttcag gatgtagatc agatgctgtg
    16201 ctgcttggca gaacaggttc tacccatttt ttgatgtacg ccccgttata atccaagtaa
    16261 ctgttttcaa aattccgttc atttccccaa agctgaacgg tttgagctgg aacgtttcta
    16321 tcttcttcgc ctgcagttga tccattatta gtagcatagt cgtaatttcg ccagttcgct
    16381 cccccgtttg tataatcact atcatcgatc aagttctctc cgtcatatgg cgagcctttg
    16441 tctccttgat agcgttcatt gctttgtgtc caatttccta acacttttgt atagtcattc
    16501 gttgtaaact tcgggtagcg atgctgttcg ttttcaactt ctccgctagg atagatatat
    16561 tcataggggt cttgcgtagc tgttggccca agacgctcca aaataccgtt gaataactgg
    16621 cgttgaatgc gctggagtgc ttgaccgctg ggtacgattg gttcgtccag cttttccatc
    16681 tcattttctt cgctacgttg aatttcagcg gaatcttctg tatcagtttc attttcttgt
    16741 aattcttggt tatctattct ttcatcaacc accacttgaa gaggtagtta cccttcgttc
    16801 acatcgggga tcttttctgt ttcaaaaatc aatgtgccgg tttctgcttc ttttgaataa
    16861 acttttccga cataccattc ttgaccatcg atcgtctcga cttttaggtc ttctccatct
    16921 tcatcatagg aggatagacc tgttccacga acgtttttta cggtaccaat cccggcggct
    16981 gccgcatcga ttgctaaacg tattctacct tcattggcac tttccttttt ttcaaaagcc
    17041 agatgccatt caagggtttc ttctttttca atatacttcc cttcaatttt tgcatagtcc
    17101 tcatctaaga atacctcatc ctcctttgtt tctgttgact cagcagctac atccagggtc
    17161 gtcatatgat agactgactg gaaatatggt ccaaatagca aactgacagt tactaactgc
    17221 aaaagcttca attttaaccc tttcacatcc ttatactcct ttcaaaaata tttgtcttct
    17281 ttcacaacaa tcaacaaaac aatgcccatc taattataat ttaatttaat tttaatt
//
LOCUS       DAZKMB010000056        17185 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000056 DAZKMB010000000
VERSION     DAZKMB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.056"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1387
                     /locus_tag="ACG8V3_001647"
     CDS             47..1387
                     /locus_tag="ACG8V3_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0300185.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1605..2048
                     /locus_tag="ACG8V3_001648"
     CDS             1605..2048
                     /locus_tag="ACG8V3_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0300186.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2552..3970)
                     /gene="nhaC"
                     /locus_tag="ACG8V3_001649"
     CDS             complement(2552..3970)
                     /gene="nhaC"
                     /locus_tag="ACG8V3_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0300187.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4373..5596)
                     /locus_tag="ACG8V3_001650"
     CDS             complement(4373..5596)
                     /locus_tag="ACG8V3_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0300188.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5627..6466)
                     /locus_tag="ACG8V3_001651"
     CDS             complement(5627..6466)
                     /locus_tag="ACG8V3_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0300189.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6483..7163)
                     /locus_tag="ACG8V3_001652"
     CDS             complement(6483..7163)
                     /locus_tag="ACG8V3_001652"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0300190.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7165..8238)
                     /locus_tag="ACG8V3_001653"
     CDS             complement(7165..8238)
                     /locus_tag="ACG8V3_001653"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300191.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8294..8532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8575..8808)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8885..9556)
                     /locus_tag="ACG8V3_001654"
     CDS             complement(8885..9556)
                     /locus_tag="ACG8V3_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0300192.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9643..12291)
                     /gene="mgtA"
                     /locus_tag="ACG8V3_001655"
     CDS             complement(9643..12291)
                     /gene="mgtA"
                     /locus_tag="ACG8V3_001655"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0300193.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12633..12807)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12980..13417
                     /gene="ndk"
                     /locus_tag="ACG8V3_001656"
     CDS             12980..13417
                     /gene="ndk"
                     /locus_tag="ACG8V3_001656"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0300194.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13439..14305)
                     /locus_tag="ACG8V3_001657"
     CDS             complement(13439..14305)
                     /locus_tag="ACG8V3_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0300195.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14465..16558)
                     /locus_tag="ACG8V3_001658"
     CDS             complement(14465..16558)
                     /locus_tag="ACG8V3_001658"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0300196.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4966 a         3565 c         3037 g         5617 t
ORIGIN      
        1 tcttaatata aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa
       61 attgaaaaaa caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg
      121 tgcaggtcta tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat
      181 tgcttttttg attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt
      241 tttaagcggt ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc
      301 tcattctgct gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc
      361 tgaagctgtt gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt
      421 atttgttttt atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt
      481 cgcagaaaca gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat
      541 ttttgctact tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca
      601 gcaattgact gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt
      661 gcttgtagtt atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc
      721 agatgatcct aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc
      781 cttttacatc attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg
      841 cacgaatgtc agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga
      901 tatcgtcaat tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc
      961 atcttcaaga attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc
     1021 tgttttaaac aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta
     1081 tcttggtgta gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc
     1141 cctttcgtat acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc
     1201 actaagaaaa ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg
     1261 attgattttc cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct
     1321 gctttacctg ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa
     1381 tgaatgaaat cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga
     1441 aaggagttgt gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc
     1501 tcttggtaaa cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca
     1561 tgacatgata gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga
     1621 agtttttgtt gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg
     1681 tctatgcgct cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg
     1741 gtgcagtcat ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga
     1801 taaatgttat gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac
     1861 gaagccaaac tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta
     1921 tcgctatcct ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg
     1981 aagaaatcat tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa
     2041 tggaatgacc attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca
     2101 agaaataaga agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc
     2161 tgcatcaagt aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag
     2221 gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg
     2281 ttctgacctg agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt
     2341 tgagctaata ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc
     2401 gcttagtttc gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat
     2461 ttggcttaat accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac
     2521 aaaagtcttg tcacaatccc tttttatgtt tttattgttc taattgcgct gtcttgctta
     2581 ctgcttcttg cggtttcaca aatttttttg agattcccca acctgtgaag gcactgacca
     2641 tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa
     2701 taccaaatgt acttgctacg aaacttccgg caattcccca aggcactaag tagttgatta
     2761 ctgttccacc atcttctagc gttctaccta atacagttgg atcaaatcct gctttttcat
     2821 aagtttcttt aaatgcatta ccaggcagaa taacagatag gaattgttca ccaataaaaa
     2881 tatttactcc gatacttgtc atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct
     2941 ttcttgatac tcgttccatc acggttgaga tcaagccaaa ttcgatcaac aatccaccta
     3001 atgacaaagt tagaataatc aatgacacag taggcatcat actttcgatt cctccgcggc
     3061 taagcagtga atcgatttgt tggtttccag tatttgaaac aaaccctgat tcgatgaccc
     3121 cagccaactt cgttaaggaa gtttgtgggt tttgaataaa aatcattcct actgctacag
     3181 cgatattcaa caaaatcgtt aaaattgccg gcaccttttt ccatgcacac agtaacatta
     3241 aaaataaagg aatcagtgac cagattgaaa tagtaaagtt tgcttctaga acatcagtca
     3301 cttcacgaat agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa
     3361 agaaagaaac aacaaatgcc gggacagttg accacatcat atgcttgatg tgtttgaaca
     3421 aatcggcatc aacaatagct gctgctagat ttgttgattc ggataatgga gacatcttat
     3481 ctccaaaaac ggcacctgaa acaatagctc caacaaccag cgctggattt aagcctagtg
     3541 ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat aacagtaaag gcactcccta
     3601 cagcgctacc tacgattgca cacacaacaa atacagaagg gacaaaccac tggacactga
     3661 tcaatttgaa cccaatgacc attagagttg gaatgatccc tgcttgtatc catacagcaa
     3721 tcaatgaacc gactaaaata aaaataaaga ttgggatgat tcctggtttg atgccattga
     3781 tgattccttc gtgcagccaa tctgtcgggt atctttttat cactgcatac aacatgacca
     3841 ttccgatcgc tgctaagatc gtgatattag gtgagacctt taatccgaca acgccaatcg
     3901 caatgcctaa aacaataacc attaatacaa gtgttgcttc taaaaaactt ggtgttcctt
     3961 tttttcccat gataatctct ccttttttag ttgttcttat gttctaaaaa agttaagata
     4021 tcggattact ccgccgcaat tcatccgcga ttcctccttt ataaaaataa aaagacctgt
     4081 tttaaactaa ggacgaatga ctccgcggta ccaccttaat taaaaacaga tctgtttttc
     4141 actttcaaac ttttattcaa atgtatggac tttccatgta attcgtcaat taggcttgat
     4201 cggttcacag cgaccaccga ctttctgaac aaaacaccta accgctactg cgtgaaaagt
     4261 tgacttgtac gaagtttacc ttccataaga tcatttgtca atgaaaaaga aaatattttt
     4321 tcatttttct gaaaattatt tttataactt tcacacacaa gattattttt tactagaaag
     4381 tagcgccctc ttgttttttt cttgctacta tttcttgatg ttcttgcttg attctttcta
     4441 atagctttgg ctctttcaac agatcaagag ctgtattggc gagtagttct gctgcaatcg
     4501 caatagaagc aaggcctttg tcacttttag cagcagcttt gaattcttct gtgtgtccgg
     4561 caatatatgt ctcagagatt gctaccgtag gctggattgt tggtatgact tggctgacat
     4621 ttcctacatc agaagaaccg agacttgttc tttgttccgt ttctatttct tcatctggaa
     4681 cacctgcttc ttttgcatgc tggaagaata agtcatcaaa agaaggagta acgatgacat
     4741 catcaactgc attttggaac aatccaaact caaaagtcgt gcccgtactt atcgcagcac
     4801 ctcgtacgat attttccact ttttcatata ctttatctaa tgtttgccga tttcctgcac
     4861 gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt ggcagctact cctccgtctg
     4921 taatgatccc atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccat
     4981 taaacacttg gatcaatgct tccaaagcat ttactccttt ttcaggtgct gctgctgcat
     5041 gggaagcgac accgtaaaat ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt
     5101 tcccataacg aaaagctgga tggacgcata acgctacatc gacatcctca aaaaaacctt
     5161 ctcgaacaaa gctgccttta gctgatccgt tctcgccccc ttcttcccct ggggtgccat
     5221 atacacggat ttctccacca gtttcatcaa tgaattgctt cactatgctg gcggctaata
     5281 cggagtaagt accaaacaaa ttatgcccgc atgcatgacc aatacctggt aaagcatcga
     5341 attctgccaa aaaggcaatt gtgggaccgg gtttattgga aatataacgt gcatcaaagg
     5401 ctgttcggtg tccagctact tctttttcta cccaaaacct ttctttttct aattgtttaa
     5461 tcaacgctgc tgaggaataa aactcatagt tacttacttc aggatgttcg tggatatcta
     5521 gagcaagtgc ttgatattct ggtaaacgat attggattag atccttcaat tggtttgttg
     5581 agattgttgt tgtcataaaa aattcctcct atgctagtca tttttcttat tttctcccga
     5641 atgtttccca tgcaggcacg cttgatcctt ttgatgtttt ctcgattact ttgactgtgt
     5701 cttctgtttg ataagcgtct actatttttt gatacgtttt attgttttca tcttcttttc
     5761 ttgcaacgat gatattcaca taaggacgtg catggtcatc cactggttct aagaagatgg
     5821 catcagaagc aggattaaat ccagcatcta cagctacacc gctattgatc actgaaatgt
     5881 ctacatctga caaagcacgt gctgtttggg aagcatctaa ttcagaaatg tctagatttt
     5941 ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta tccactttga
     6001 tcaatccagc agtttgcaat aacagaagag ctctgccgcc atttgtaaca tcattaggga
     6061 ttgctacttt tgctccgtct ttgatatcag ctacatcctt caaagtgtta gaataaattc
     6121 ccagcggtgc attgacagta tttccgatcg atactaaatc agttccgtgt tctttattgt
     6181 aattgtctaa aaagaattga tgttggaaag aattcaaatc aatcgatcca tctgctaaag
     6241 ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc caaattaatg ttctcctttt
     6301 tcaaacggtc tttcacgttg tcccatacat ctgtatcttc tccaataatc cctagtttta
     6361 cgatagtttc ttttttgtca gttgtatctg cttttccttg gctacaccct gccaaccaaa
     6421 taattgctac aaatgtaatg actacaaaac gaataatttt tttcattttt tctcctccac
     6481 tttcaatgtg taattttttt aatcagttga ttactgataa attgacttaa aaaaactaag
     6541 attagaatca ataatgtagc cacaatcgtt acatctgttt ggaatcgatt ataccctctc
     6601 gatatagcaa gattccctaa tccgcctgca cctactgctc cagccatggt agtcagtccg
     6661 atcacgttga tgatagtcaa cgccgagacc cgaatgatac caggcaaacc ttctaacaaa
     6721 tacacacgta aaatgatacc gattggactg gttcccatcg cttctgccgc ttcgatcacc
     6781 ccaggatcaa cttctagcag tgcgttctcg atttgacgag caaaaaacgg aacaactcca
     6841 aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta
     6901 gtcaatgcta aaagagaaag caagataatg aaagggatgg aacgaatgat attgatcagc
     6961 ttatcaagta tttcaaatag cagacggtta gctagtaatc cattagggcg tgtcactact
     7021 aacgtcacac ctaagatgat tccaatcatc ccgccaatga atgctgtcca aaaagacata
     7081 tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac attgggcaaa
     7141 tatgttttaa agaaatcagt cattttatct tcctccttcg atcactttga tacgactatc
     7201 taaatcgttt ttttgtaaaa tatttatttt tacaccagat ttgatcaaat aatccaatgc
     7261 ttttttccgc tgtttggttt ctccagctaa agtaacgatc aaactcccta acggcgtttc
     7321 ttgtaataac tctacattgc catataaaat attcgctgtt acttgatatt tgctgtacag
     7381 atgagcgatt agaggttcgt tcgtctgatt tccaatataa cttaattcca ccagccattt
     7441 attggcgatc tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg cttggtcaag
     7501 atgagtagct gttcgaataa aatctttggt caattcttct tcaggattgc tgaagatctg
     7561 gatgctgcta cctttttcaa cgattcttcc atcttccata accgctacct tgttgcagat
     7621 ttctttgaca acttgcatct catgagtgat caggacgatt gttaaaccta actggcgatt
     7681 caattttttt aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg cactggttgc
     7741 ttcatcacat aataatacct taggttcatt cgccaaagct cgtgcgattg ctactcgttg
     7801 tttttgtccc cccgacagtt gtttaggata agcagatgct ttttcttcca aaccgacaag
     7861 ttccagtagt tcattcactt tttgacgacg ttcctgtttc gattttccag aatactttaa
     7921 agaaaaatca acgttgtcaa aaatagttcg gctttccatt agattgaaat gctgaaagat
     7981 cataccgatg gcctttcttt cttttcgcaa ttctttggga gagagctgac tcagattcgt
     8041 ctgattgaca atgacttctc ctgctgttgg tttctgcagc aaattcatca cacgaactaa
     8101 cgtacttttc cctgcacctg agtaaccgac gattccataa acatcgcctt tttcaatcgt
     8161 taaatctaca gaatctactg cctgtatctc tttttctttt tgtttgaaac atacagaaac
     8221 atctcgcaat tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg
     8281 aaaatgtcat tttcttgagg acgctttttt gcgtgttacc acctcatgtt caagctgtgt
     8341 tcacacacaa ctcctcttca agtacgatca tactctagca agatatcggt cgcagccgtc
     8401 atcgccttac atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc
     8461 ttgcttcttc tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt
     8521 caatgcaaat aatattcaaa aaaaaagtcc ttttactaac ataaactatg tcagtaaaag
     8581 gacgacgtat cgcggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc
     8641 aaaaagagag attggcgttc gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg
     8701 acaaattatt ctccaaggcc attttcagct tttcttactt gactcttctc accagccaga
     8761 gcctctctgc aaagtttcca aacttacttt ccttttcaac gaaatcgata ttttgttgtt
     8821 gggattaatc tacatgagga aaactgaaaa gtcaagtacg aaattcaaat tattttttct
     8881 atatttattt ttctgttttt ttatttaaca tttcaataac agttcgaaga tcttttcctt
     8941 ccgcaagaag tttcaatttt tccttttctt cgacggtata atttcggaca aaactggaat
     9001 caggtatcaa aacaacgcct aatcctgctc gtgaagcagc tgttgctcct actaaagaat
     9061 cttccactgc aaaggctgaa ctagcttcta atcctgttcg ttccaaagct gtcagataag
     9121 gaatcggtga aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg tccaagtaat
     9181 cagctaattt aaagtgatcc aagatatctg ttgctctttt cgtaacagtg gtcgtagcaa
     9241 tgcctagttt catatgacgt tcttttaagt ctgccaatat ttcttttgca tatggtttta
     9301 agtggatggc tccgcttgtc agctgttgat aaaaatattc ttcacgcttc gctcggactt
     9361 cctgtacttt ttcagggcta cctgctgctt tcactaaaat atcaaaactt tgtttccagt
     9421 tttttccgcc ccaagcgtca acgatttctt ttgggatatg gatatcaaat tgctgaaatc
     9481 cccaaagcca gccatcacga tacgtttttt ctgtgtcaac taacaaacca tctaaatcaa
     9541 atattgcacc tttcatctat acattaatcc tttccaaaac aatacacata gtttagcacg
     9601 actggtccaa aaagaacaga agcaaagaac cagccttctc gtttataaga gttcttggaa
     9661 tcgacggata tagattttct tcacagctgt cacaagcgcc agataagcga tgactgtagc
     9721 aaataaccat aaccagtaat tgccaggaag tggcatcaag cctaattcag caccaaaact
     9781 tgtaaaagga aggacagaac cgacaataat tcctaatgta gtgataacaa acatggcaaa
     9841 tgttgcgttg ctttggataa atggtacttt cggtgtacgc agtgcatgga gcactaaggt
     9901 ttgtgtccat agtgattcga caaaccatcc tgcatggaat aatgcgatga aaatcactcg
     9961 ctgtgaacta tccaatgtat ggaaacttcc gcccactaca gcaggacaaa taacaaaata
    10021 catcaacaag taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt aaaccatgaa
    10081 ttccccaata ctagatgcat tccatttttt tggctcgtcc aaatattctt tatccatccg
    10141 atcccatggc aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca aaagttgtaa
    10201 aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa acatattgcc
    10261 aaaattagaa cttgctgtag ccttgatata tttcatgata ttcccaaaag tttctcgtcc
    10321 agaaagaagg cctctttcca aaatcgtcaa atctttttct aaaaggatca cgtctgcaga
    10381 ttctttcgca atatccacag ctgtatctac agaaataccg atatcagcca ctttcattgc
    10441 aggagcgtca ttgataccat ctcctaaaaa tccgacagta tgaccatttt tacgtaaagc
    10501 tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg aatacttcat ggtcttcagc
    10561 tacttgtgct aattcatgat cactcatttc cgagattttc tcaccggtaa ccaattcatt
    10621 gatctctaga cctacttgac ggcacactga acgtgtaact aattcgttgt ccccagtcag
    10681 aacttttaca gctacaccat gttctttcag agctttcaac gcttgtttcg ttgtttcttt
    10741 tggtggatca aggaaagcaa gataaccaat caaaaccatg tctgactcat ctttgactga
    10801 aaattctcca acgacgcttg gattggtctt ttgtgcgaca ccaatcactc gtaagccatc
    10861 ttcattaagt gactgtactt ttgcaagtac ggcttctttt cgttcttttg ttaatggtgt
    10921 aacagtaccg ccaagatcaa taaatttaga aatgttcagc atttcttcaa tcgcaccttt
    10981 tgtgatcatt tgtgttttac cagctggatc agcaaccaca acactcatac gacgacgttc
    11041 aaaatcaaaa ggaatttcat ccactttttg ataattgatc ttatctgtat ctaaagtttc
    11101 tgttgcttca tcaatgatag ccacatctaa caaatttttc agccctgttt gatagtagct
    11161 atttagatac gcatggcgca aaacacgatc atcttcattt ccttcacaat ccatatgata
    11221 ttcaagaatg attttatctt gtgtcaatgt ccccgtttta tccgtacata acacatcgat
    11281 tgcaccaaag ttctggatgg cattcaaatt tttgatcact gttccttttt tggccatcac
    11341 acttgctcct ttgaccaaat tcgtcgtaac gatcattgga agcatttcag gtgttagacc
    11401 aactgctaca gatagtccaa aaagaaaagc ttctagccaa tccccttttg taagcccatt
    11461 gatgacgata actgctggag ccatcaatga cataaatttg atcagtagca tggatgtttt
    11521 atggatgcct aattcaaagc ttgattgaat tggtttttca gatagcgttt ctgctacatg
    11581 accaaatagt gtttcatttc caacagaaac aatgattcct tccgcacttc cactgatgac
    11641 attacttccc ataaagacaa gattttcgta acttgtttcg ctttgctgat cgtcttcaca
    11701 tactttcgca tgcttttcta ctggatagct ttcccctgtc aatgctgctt gggaaatgaa
    11761 taaatccttt gcggacaata aacgcatatc cgctggtatc atatcccctg ctgaaagccg
    11821 aacaagatct ccacagacga tttcttccat aggaatctct gtgtactttc cattacgttt
    11881 tacagctgcc gttactttca caagtgattt cagtttttca gccgcttgat tggaacgtac
    11941 ggactgaatc aacgtcattg tcccgctgac gataaccata gtgaagataa tggctacacc
    12001 gaaaagatct ttatctccag gagctgctaa tacatattcc gtgatgaatg aaataattcc
    12061 taatgcgatc aacactaatg taaatggcgt gatgtatgct ttcaatattt ctacaatgaa
    12121 tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg aacgttcgat
    12181 ttgttcttct gtcaatccat ttgctgatgc atggaatagt tcaaataatt cttgtttcgt
    12241 ttttccagca aaagctgcat agattttttg tgtatctttc tttttttcca tctcaatctc
    12301 tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg tctttagcta ttcatcggat
    12361 cgatagagaa aaaactgaaa ggggctgaga acagatagat aatagccctg gcctttcctc
    12421 tagatccttg tttttcgaat gactgtcatc catttgttct catctccttt actaagtaat
    12481 tttaattcat gacttccaat catttttcga atagttattt ttccaattag accacctcct
    12541 ttttattttt caccgatggg gatttgcggc aacaaaaaaa cctctcgcca aatactcagc
    12601 gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg agttttgaca
    12661 ctgtacaacg taaaaagcag atacctgctt ttagctacct tatgaaaagc cttatctcga
    12721 caattcctgt tctacccatt ggcgtctctc gacgtttttg ggcagtagcc tatgtttgta
    12781 taggagcctc acctaacaaa tcttattcgg ttttccttcg ttaatataac aactaaaaaa
    12841 gaggatgtca aatcaatttt ccatgttttt catacttttt ttaaaataca cacgttatat
    12901 tcctgtctca cgcggtttta tttgtctcat tttccacctc ccgctataat agaaatatag
    12961 taggaaacga ggtgctgtta tggaaagaac acttgttatt atcaagccag acggcgtgag
    13021 acgccattta gtcggaagca tcatccaacg ttttgaagca aagggactgg ccattgctga
    13081 aatgaagttt gaaactatga ctcctgaatt agcaaaagaa cattatcagc acttgactga
    13141 gcgcagtttt tttgatgaac tgatcgatta catgacttcc ggtcctgtcg tttatcttgt
    13201 gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa caaaagctgc
    13261 cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg ccggggactg aaaacatcat
    13321 tcacgcttct gattcccgtg atgctgcggt aaaagagatc gcccgctttt tcccgccatt
    13381 cgatataaac aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa ctgctggtct
    13441 agttgctggg ttcagcaatg gccagcagtt ttttctctaa ggacctccgt tgattctttc
    13501 ctaacatata ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt tcttccccta
    13561 aagcagcaga aatcaatctt tgataataat ctgccatcaa tgtttcatag atcactgtac
    13621 ttctttcttt atgtgctaag ctccgtttca tatcttcatt cacactacgt gcctttttca
    13681 accgaccatt ttctatataa aacgaagtca ggttcattaa aaaagtaaag agatactgct
    13741 catgattgtt caactgtgct aatcgtcgca ttcttttttt gattttcaaa taattgttct
    13801 caatgaaccg atcactgaat atccaaggaa aatcagctag aatagatagt tcaaattgac
    13861 catagtctgg tatctgcatc aagtattctt caatgattcg gcattcataa cgtaaaaatt
    13921 caaattctgt ctcagatact tctgtttttt cgaccatgtg aagattcaaa accaaataat
    13981 acagatcttc tgttttctta aaatgcctac ggttattgct gatctcttta taaggatcaa
    14041 agctgtcttc ttgtacccga tctagcattc gtcttttttt ctttaacttt attggttcat
    14101 ccagctggtt tcgcatccat tcatattctg caagtgtcat gttcattcgc tctaaaaatg
    14161 atctcaatgt tacaaatgac atttttttca ctgaatttgt ctcaatcccg actagagaag
    14221 tcttaaaaca gattccttcc gctaattggg actgtgtcaa cttcttgttg actcgaagtt
    14281 tcttgatcaa ttcaccatca tacataatag ttctcctttt taatttaaat ttttccacat
    14341 tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa aaataaaaaa atgttttttt
    14401 cataaaaaat accaaccttc aagcattaga atgttgtcta actactgaag gttggtttga
    14461 ttgtttattg attatttttt tgctgttcaa cgtctgttct tgttactgga tataagttgg
    14521 ttgttgtttt attaccattt tttgaaaaga cacttgtcga atgttcgcct ttttgctttt
    14581 cgattgcttc attacgttcc gcttcatttt tatagtcata ttttttcgga tcaatcgtct
    14641 caaatccgtt tggcacataa aaccgcaaga gattttcctg attcagctta tctgaaagac
    14701 ttaacgcagt cttgactttt tcctgatctt tggagatttc ctcatcgatt cctgctgctt
    14761 tcgtatcgat gatttctcct gtctgattat tgtaagcttt tcctcctagg acggtgtatt
    14821 tgggtgtcac aaagttccca tttcgaaaag caactacttg atcatgctga gaagataata
    14881 agtctgtacc aaaatgaaga taatctttat catcgattcc caatagatgt agtaatgtcg
    14941 gcaaaacatc gatttctcca ccgtattcat gattgactgt ccctttagta taaccaggca
    15001 tatggatcat caatggcact cgttgcatct gtgcgttgtc aaattcatcc catgtatctg
    15061 gatctttacc aagagcgctt gctaaatctt tattatctgt attcgagatc ccaaaatgat
    15121 ccccgtaaat cacaaagatt gaattttggt aaataccaga ttttttcaaa tacgtaaaaa
    15181 attgttccaa tgattgatcc agataatgtg ccgttcgaac ataattatct actgtgctat
    15241 tgccagtatt caaagatgga aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt
    15301 tggttacgga aagaaatttc gtgtaaaacg gctgttgtag atgctcaaga tattgggcag
    15361 attctctgaa taaatcttta tctaatatcc cataacctaa cgtttcgtcg gattcatcaa
    15421 aataagatcg atcaaagaaa ttgtcataac ctaagttttt atatacatga tctctattcc
    15481 aaaaactagc tacattccca tggaaaacag cgcttgtata gccttgcttt tgtccaagga
    15541 tcgctggtgc ggcttgaaag acattgtctg acccaagttc tgtgaataat gagccttgag
    15601 gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc acttgtcttt ccctgtccta
    15661 cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt ctggaaaata ctatttaaaa
    15721 acggtgtgac ttcttgtccg tctaccttca tattaatcaa aaactgctgg aaactttcta
    15781 aatggagaac gatcacattt ttccctttag cgatcccaaa tgtttcggga tttggctctg
    15841 cataatgttt ctttgtataa tctaaaactt catcaatccc attactgctt gcatgcgcgc
    15901 gaacactgct agtcattcct gttttgattc cgtcataaat agtatacgca tcgattccta
    15961 gatatttgac aatatatgaa cggtcaaatg ttcgctgcag caattgtgga cggttggctt
    16021 cgctcagcgc tagatttcct gaaaaaataa gccctgaaag acaggtaaca gcgatagcca
    16081 tcggtttgcc gacagggttg cttttgatag gtattttctt gaccttcaac caaaccagta
    16141 gcccaatgaa cacagcgata tccagccagt agatgacatc atggggcttc atcaaagaaa
    16201 aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc taatacagat ttgactgtaa
    16261 tgaaatctgt gaattcccga taaaaaataa tattcaagta caacaaaagc gtatttaaaa
    16321 tatcaatgat caaaagaata gctaacgctg gctttggctt tttaatatac aaagaaaggc
    16381 caaaaaacaa taaggtagtc gcaattgggt tgatccacaa gataaagtac tggatcgtac
    16441 cttccacacc taatgaaaaa tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga
    16501 aaacagctgt caaaacgatt gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt
    16561 atatcacttc tctctattca cattttttct tattttgctc ctgaaaaatg aaaatttcct
    16621 aaaaaagttg ttatcttaag atttttttat tctttctaaa gattattccg tccacttttc
    16681 acagcataaa accgctgcga tgtaagcgat taaactaatg aagagataaa agtcttgtgg
    16741 tgaccgcttc actccacggt attcaaaagc cagtttctcg gtattcgtac aaaaaaagag
    16801 gctgtgacaa aattcttgtc gcagctcctt atttcttgat gcaggacagc tttttcacaa
    16861 cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc acaagtcaca atatttataa
    16921 aacatgagaa gctgtttttt gaaattgttc cttgtggctc agagttaaac ggcaggctca
    16981 agacctcttg atacacggtg ttcaatcagc tgaccctcgg tattagatta tgtcaggacg
    17041 ctaattttac aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    17101 aaccaaacga aaaatataag gagctatttt ttgaaattgc tccttgtggc tcagagttaa
    17161 acggcaggct caagacctct tgata
//
LOCUS       DAZKMB010000057        16516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000057 DAZKMB010000000
VERSION     DAZKMB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.057"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG8V3_001659"
     CDS             complement(<1..1835)
                     /locus_tag="ACG8V3_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0300197.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG8V3_001660"
     CDS             complement(1832..2536)
                     /locus_tag="ACG8V3_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0300198.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG8V3_001661"
     CDS             complement(2548..4857)
                     /locus_tag="ACG8V3_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0300199.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG8V3_001662"
     CDS             complement(5052..5516)
                     /locus_tag="ACG8V3_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300200.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG8V3_001663"
     CDS             complement(5516..6142)
                     /locus_tag="ACG8V3_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0300201.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG8V3_001664"
     CDS             complement(6149..6514)
                     /locus_tag="ACG8V3_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0300202.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG8V3_001665"
     CDS             complement(6504..6842)
                     /locus_tag="ACG8V3_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300203.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG8V3_001666"
     CDS             complement(6832..7158)
                     /locus_tag="ACG8V3_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0300204.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACG8V3_001667"
     CDS             complement(7136..7417)
                     /locus_tag="ACG8V3_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0300205.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACG8V3_001668"
     CDS             complement(7419..8783)
                     /locus_tag="ACG8V3_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0300206.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG8V3_001669"
     CDS             complement(8796..9365)
                     /locus_tag="ACG8V3_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0300207.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG8V3_001670"
     CDS             complement(9337..10524)
                     /locus_tag="ACG8V3_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0300208.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG8V3_001671"
     CDS             complement(10588..12315)
                     /locus_tag="ACG8V3_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0300209.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG8V3_001672"
     CDS             complement(12312..12764)
                     /locus_tag="ACG8V3_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0300210.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG8V3_001673"
     CDS             complement(12876..13256)
                     /locus_tag="ACG8V3_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0300211.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG8V3_001674"
     CDS             complement(13253..13639)
                     /locus_tag="ACG8V3_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0300212.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG8V3_001675"
     CDS             complement(13641..13919)
                     /locus_tag="ACG8V3_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300213.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG8V3_001676"
     CDS             13967..14695
                     /locus_tag="ACG8V3_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0300214.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACG8V3_001677"
     CDS             complement(14841..15317)
                     /locus_tag="ACG8V3_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0300215.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACG8V3_001678"
     CDS             complement(15277..15633)
                     /locus_tag="ACG8V3_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300216.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACG8V3_001679"
     CDS             complement(15611..15796)
                     /locus_tag="ACG8V3_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300217.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACG8V3_001680"
     CDS             complement(15797..16069)
                     /locus_tag="ACG8V3_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0300218.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACG8V3_001681"
     CDS             complement(16066..16266)
                     /locus_tag="ACG8V3_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300219.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT         4649 a         3480 c         2560 g         5827 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctact
//
LOCUS       DAZKMB010000058        16275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000058 DAZKMB010000000
VERSION     DAZKMB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.058"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..879)
                     /locus_tag="ACG8V3_001682"
     CDS             complement(16..879)
                     /locus_tag="ACG8V3_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0300220.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1075..3174)
                     /locus_tag="ACG8V3_001683"
     CDS             complement(1075..3174)
                     /locus_tag="ACG8V3_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0300221.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3394..4503)
                     /gene="rpoD"
                     /locus_tag="ACG8V3_001684"
     CDS             complement(3394..4503)
                     /gene="rpoD"
                     /locus_tag="ACG8V3_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0300222.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4527..6368)
                     /gene="dnaG"
                     /locus_tag="ACG8V3_001685"
     CDS             complement(4527..6368)
                     /gene="dnaG"
                     /locus_tag="ACG8V3_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0300223.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6550..8865)
                     /locus_tag="ACG8V3_001686"
     CDS             complement(6550..8865)
                     /locus_tag="ACG8V3_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0300224.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9050..9910)
                     /locus_tag="ACG8V3_001687"
     CDS             complement(9050..9910)
                     /locus_tag="ACG8V3_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0300225.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(9969..10601)
                     /locus_tag="ACG8V3_001688"
                     /pseudo
     CDS             complement(9969..10601)
                     /locus_tag="ACG8V3_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            10768..11529
                     /locus_tag="ACG8V3_001689"
     CDS             10768..11529
                     /locus_tag="ACG8V3_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0300226.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11555..14146
                     /gene="mprF"
                     /locus_tag="ACG8V3_001690"
     CDS             11555..14146
                     /gene="mprF"
                     /locus_tag="ACG8V3_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0300227.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14334..15521)
                     /gene="tuf"
                     /locus_tag="ACG8V3_001691"
     CDS             complement(14334..15521)
                     /gene="tuf"
                     /locus_tag="ACG8V3_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0300228.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            15889..16245
                     /locus_tag="ACG8V3_001692"
     CDS             15889..16245
                     /locus_tag="ACG8V3_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0300229.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4447 a         3377 c         2872 g         5579 t
ORIGIN      
        1 acatgcaagg gattctcaat tagatttttt gactagtttt gtcacaccgt tttcttgtgt
       61 aatcaatcct tgtttcaaaa gattacctaa tgcgcgctta aatgctcctt tgctgatacc
      121 aaaagctttc atgatttctt ctggatccga tttatctgta aagttgattt gctgatctgc
      181 agaacgttct aaaatggtta aaatcatttg tgcatcatct gatatagctt catatgctct
      241 tggcttcaat gaaagattca gtacaccgtc tggtcggaca ccaatcactc gaccttttat
      301 ctgctcgcct agacgaggtt cattatatcg ttcagaagga tggataaacc caagatagaa
      361 atcttccgtt aaaacgtacg tacctgttaa tttcaagcgg tatgctgttc ctatcacatc
      421 tttgtttttc atttcttctg ttccatattt gcttaaagat ttgaatattt tttcatctgc
      481 taaactgccc caaatacggt cttttgcatc aactctaagg gaaatcatca agcgatcccc
      541 ttttttaggc catagctctg tcattgtcgg caattcatca agagatacga caaagtcttt
      601 atctggaaga ccgatatcga caaacgcacc taaatctttt cgtgtagcag taacgattcc
      661 aaaagcgtaa tgaccgatac ggcttttagg aatttgtgta gtgatccgat tttcatgttt
      721 ttgattttgg taaccgaatc cttcaactgc ttcaccgatt ttgtgttcac cttctgattt
      781 gcttaagcca aaggtttgtc cgtttttttg gacaaagtag tttttttcat tttcatcaat
      841 aataatacct gtaaatatag ttgctaataa atcgttcatt tttttctccc tctatagtga
      901 aaggttttct taatacattt ctttgctatc ataacataat gttatctact tgaatagctg
      961 agccttggtt tggaaaggtg aaaaaaggag tatgacaaca gttgtgtcat actccccttt
     1021 ttcgaataaa cggtgttaaa cggcaggctc acagcctctt tcatttgcct aaaattaatt
     1081 gctcccattt cctccgaacc atccagtttt acagttgatg gttacgatgt aaaatggcaa
     1141 agcactttct ccaatgcctt tcatcaatgc agattttgtt gaagtggcat atagattata
     1201 atagccttcc ccattttcga ttctggcttt ttctaatctg taacctcctt caacgatata
     1261 tccacgttgg atacatgtag caatcacttt gctttggaca cctggtgccc acgtccatgc
     1321 tggattagat gcatcggcaa tatcacttgc tctggcagat ttgattggct gattcgctga
     1381 gtttgaagag gaaggggtcg aggatgccgc ttcttgctgt gctgcttgtg cgaccgcagc
     1441 ttgttgagcg gcttgagctg cagctgctgc ttgagcggct tgttttgctt gttcttcagc
     1501 tgcttttttc gcttcttctg cttttttctt ttcctcttct tcttttttct ctttatcagt
     1561 gagcatctta tctacttgtt tcaattgttc attcaactga gcagatagat ctgtattttt
     1621 cactttttct actgcttctt ttgccgtctt gtattggtcg cgtgttgctg aatccactac
     1681 ttttccgtct ttaaagacta tagctgtttt ttctttagct gtttgcaatt cagcatattg
     1741 atttttagct tctgtttgtg cttgttgaat caattttcca aaatctgtgt tatccgtttc
     1801 gattgcttgg atctctttat ctgctttcaa cttgatgtca tctgctagat gatcatcgac
     1861 gatggcaggt gctatgaacc actcattcac tttttggata tctcggatct tagattggat
     1921 atcttggtaa gttgtttcta actctgaata tcctttttca ttcttaagac tggataaatc
     1981 attttttagt tttgataaat cttgatgaat catatctgca cgaataaatt gatggtgatc
     2041 atctgtataa aaggcagcaa gtttctcatg aagttcttcc atttgttgct gtctttttgc
     2101 ttctgctgct acttcggctt ctactttttg atgatggtca taataagccc agcttcctcc
     2161 accgagtaga agtactgctg ctaaaacgag ataaggtact ttttttgatt ttttcttttg
     2221 cgctttatct ggatctgttt tttctaagtt tgattcttga tgtgtttttt cagtatctgg
     2281 cacgattgtt attgatttcg cttctgcttc aaacagctta ggctggttct cttctttttc
     2341 tatttttgct tcaagatcag taccctcttt gcttacagat tgattttcag catcggattc
     2401 actatttatt tcatcgggct ctgttgactt tttgtctgtg ttttcgagta tttctttttt
     2461 ctctgcttcc ggcagtttat tcgttttttc cttttctaat tgtttttcag taaatacttc
     2521 agttttttct gtgatatccg tttttacgtc ttttattttt actgtttctt tacctgtggc
     2581 gttttctcta tcagagtctt tttcagtttc ttgttccttt tctggctcta ctttgttttc
     2641 ctgtgcaatt ttttcactga tcattttgag cagctctttc gcgtgctctt cttttgtatc
     2701 ttcacgatgt gctttgatat aagcggatag tatcgaattt tcttcctcat tatcgacgat
     2761 ttctttcagt tcagctactc ttcttttttg ttttgattca agcgtttctt cttgattctt
     2821 tcctgattct tccgaagtct catgaacagt tgctttcttc tgttcatttt tctctatatt
     2881 agttacttga tcgtgagcag gtggttcttc gtcttctggt tctgcttttt cgaataattc
     2941 cattacagat tctagaggta aatctttcaa ttccgaccat tcgattggat cgtttaattc
     3001 tgtatcagga aagactgcat cttcagttga tttatcctgc atagctgcat ctgtatttgt
     3061 ttcttcaatt gattctttta tttgctgatc ttctgcagtt atttgatgac cgcagtctgg
     3121 acagatagta ttctgctcca tttttttcga tccgcaattt ggacattttt tcatcactta
     3181 gcgatcggct cctttactag gtttacgcta ccatttaatc tatcatattc tatacgtcac
     3241 gccaatagct tatttttagg atttacttaa aaataatgaa tgaatttcct tcctatagaa
     3301 aaaaaagctt gagactgcgt agtctcaagc tttttttctt ttaaaataaa ccaaacagtt
     3361 ttactgattt ctcttctggt caatagtcca cgcttattct aagaaatctt ttaattgttt
     3421 tgaacgagaa ggatggcgca attttcttaa tgctttagcc tcgatctgac gaatacgttc
     3481 ccgagtaaca ccaaatactt tccctacttc ttccaatgtt ctcgtccggc catcatctaa
     3541 accaaaacgt aagcgaagga cattctcttc ccgatcagtc aatgtatcta gtacatcttc
     3601 caattgttct tttagtaact cataagctgc gtgttctgct ggacttgttg cttcctggtc
     3661 ttctatgaaa tcacctaagt gagagtcatc ttcttcaccg attggcgttt caagggaaac
     3721 aggttcttgc gcgattttca agatttctcg gactttttct gttggaagat ccatttccgc
     3781 accgatttct tctggcgtag gttctcttcc aaggtcttgc agcaattgac gttggatacg
     3841 tatcaattta ttaattgttt caaccatatg aactggaata cggatcgtac gtgcttggtc
     3901 agcgattgct cgagtgatgg cttgacgaat ccaccatgta gcataggttg aaaacttaaa
     3961 tcctttgcgg tagtcaaatt tttcaactgc cttcatcagt cccatgtttc cttcttggat
     4021 caagtcaagg aactgcatgc ctcgtcctac ataacgttta gcgatactta ctactaaacg
     4081 aaggtttgct tctgccagac gctgtttcgc ttcttggtcg ccttcttcga ttttcaatgc
     4141 taacgctact tcttcttccg cagttaaaag ggagacacgg ccaatttctt tcagatacat
     4201 acgaaccggg tcattgattt ttacaccggt tggcgcagaa agatcttctg tttttgcttg
     4261 ttcagctttt ttctcagcac tcttcaagct gtgaattgat ggatcaccat tttcatccac
     4321 cacactgata cctgcgtctt caatcttttg gatcaatttg tccatttcat ctgcattcaa
     4381 agtgaatggt gtagcaagct gattggataa atcatcatat acaacttgtc gtttgggttt
     4441 gttttctttg atgaaagctg ctactgctgc ttcatacgtt tttttatctg ttccatttgc
     4501 cataaaagaa ggcccccctt gagtagctat ttggcttgtt ttaactgttt cgttaaattg
     4561 atgatttcaa tcgtcaattc aagctcaagc tgtttgtttc cagttcgtct ggcttcttgc
     4621 tgcatttgtt tttttaatgt tatttcatca gctaagccag atttgctgat cacatctagc
     4681 aagtcttgga tttctcgtgg cgaactttct tctgccaccg gtaaatattc gatttccacc
     4741 accaaccgcc ttaaattttc atcttgcaaa aagtctaaaa actctgcaag gttgaattca
     4801 taatgttgtg agacatatgc atcgaataaa aaataaactt ctgcatatgc atcatgaata
     4861 aactgaaatt ctcgttcttt taaaatatta cggacagaaa cttctttaaa tgcacgatat
     4921 aatagaatct gctctgcctt ttctacttgc gtcaaaggtc tctttttccg tgcagtttct
     4981 tgtctcgtaa cctgagtgtg ttgactttcc tgaggttctt gacgattttg ctgatttgtc
     5041 cttcgaagat cgcgcagttg tttttgtatc gtctctctgc cgatctgaaa ctctgttgac
     5101 agttgagata gatacatatc ttgttcgatt ggtgaaacaa ctcggctaag ttcaactagt
     5161 aattcttcga ggtattcgat cttgtctttt tcattatcta aatttttccc ttgttttaaa
     5221 taacgtgatt taaaagtaaa gactgtttcg cgcccatgag ccactaattc ttggaaagat
     5281 gcttctccat acttacggag atattcatca ggatcaagct tttctggaac gactacgaca
     5341 gaaagtgcta atcgactgtt ttgccgaagc aattcaatcc ctcgattggt tgcttcaaaa
     5401 cctgcgttgt ctccgtcata gcaaaagacc aattctttcg tcagcttttc catttgttgg
     5461 atctgttgag ctgttagact tgtccccata gaagctaatc cagttttgat accagcctgc
     5521 catgcagcaa tcacatccat aaacccttcg aacaacaaaa tttctccgtt tttccggata
     5581 tcgcttcttg ctttatcaag attaaataaa acttgtcttt tattgaacag ttctgtttct
     5641 ggactattca agtatttggg ctgatctttt tcctgccctt tttcaggtgc aagccagcgt
     5701 cctgaaaaac cgatcgtctg gccttgagaa ttacgaagtg gaaacatcac tctttgataa
     5761 aaacgatcag acatacggcc atcatcatgc gtgacaaaca atccagagcg ttctaacaat
     5821 tcatttgaaa ttgattcatt cttgaatact tgctgaagaa aagaacgctc attaggtgca
     5881 aaacctatgt tgaattcttc aattaattct aaagacaacc cacgactttg caaatattct
     5941 aaagctggct gtccagcttt tgtatgcaat agcatgtgat gatagatttc cgctgctttt
     6001 tcatgagcag caatcagttt ccccgtagca gattcaggat ttcggacagc tatagtacgc
     6061 tgacgccatt gatcttctaa cggtatctgt tcgaagtctg ccacttttac cactgcttct
     6121 ggaaaactta gaccttctag ttcttgaaga aaagtaaaca cgtttccgcc tttaccacaa
     6181 ccaaagcaat gaaaaatctg cttgtcttct gcaacagaaa aagacggtgt acgttcttca
     6241 tgaaaaggac acagccccaa gtaattttta ccagactttt tcaactggac atattgtcca
     6301 atcacctcaa cgatattcgt ttgactgcgt actgtttcga taacctcttg aggaatccgt
     6361 tgagccattt tttcaccccc tgctctattg atatcttttg ggtttaacag aagatatttc
     6421 aggagaataa ccggtaaaaa atcgcactaa ctgaaatcag tgcgatttta aaggacacaa
     6481 ttatacatgt ttgattgtat catatttcat tgctttttca agtataacac atttctatct
     6541 cattaagcat caatttgcta ctcttttcag taactgattt tgaatatcag ccttccagta
     6601 caatactcga agaacaatat ttacgagcat gaatacaaag aagtatccta cgctctctcc
     6661 caaccctaag tttacttcca aaattccatg gaacagcatt gcagctacat atatccctac
     6721 gatcgtcgtt actgctagga agatgcgcaa tgggttgaag ggataacaag cttttacaac
     6781 tgccatgcaa ctgatcccaa tcagcaagta atacatcaaa gtagtagttt cagcatttcc
     6841 ccatgcgaat gtttttccaa gtagatacac cgcaatgata ttcactacta caagcaaggc
     6901 atttggtaaa gcattaataa gtgctgtagg caaataacgg taagtgattt ttcttttatc
     6961 ttgttcaaaa gataaaaaga aggaaggata cccttcaatc gctaaatcaa tcagcgtgat
     7021 ttggataggg ataaacggaa atccaattgc tgtaacggca catataatcg ataaaataaa
     7081 tgaataaatc gttttgataa aaaagatgcc tgagactttc gtcacattgt tgaccactcg
     7141 gcgtccttca aacaggactt ctggcaatgt agtaaaatca gagtccaaca ataccagatt
     7201 cgcaatctga cgtgttgctc catcaccttc tgccatagca atgctgcaat ctgcttctct
     7261 taacgccaaa acatcattca caccgtcgcc tgtcattgca accgttctgc cgctttcttt
     7321 caattcattt accagcaatt tcttttgctg cggactcact cgtccaaaaa ccgtatagcg
     7381 atgagcagct tctcgcactt ccatttcttc agtaatttga gaaagatcga catacgcatc
     7441 ataattcggt aatcctgcac gtctagcgat attggagaca gtaaaaggat tgtctccaga
     7501 aatcactttc agatccacac cttcctcatg aagataagct agtgtttctt gagcgttttt
     7561 tcggattgga tcatcgattt caaacagagc aaccggctca acaaatgctg gtttttcttc
     7621 gttcaattgc tgatttggag cgatccccaa cataagtacg cgatagccat tcgcttgggc
     7681 tgtttttaga ctgtccggaa gccgatcttc ttccaccaaa cgttcaggcg ctcctacgta
     7741 cacgactccc agttctggaa attccatcgc gccccatttg cgatcagaag aaaatgcgac
     7801 tttgtttgtc acatcatacc gattagaaag ctcaaaataa tctctcatag cccgcatagt
     7861 gatgttgtta tcactgcttt ctgccagata cgtccccata atttctggga tggctttctc
     7921 gtacagcgga tgtagtgctt ctacccgctg gatactcatt ttcccttcag taatggtccc
     7981 tgttttatcc aagcataatg tatcgacatg ggccagcgtt tcaattgaat acatgtcttg
     8041 aacaaggatt cgtttttttg ccagtttggt tacccctgtc gttaatgcaa tacttattag
     8101 caataccaaa ccctttggaa gcatgcctaa caaagctgct gcagatgcaa taacagaaat
     8161 tttgatagaa gcatcgcgga tgaacagtgc ttccaaaaat agtatgatcc ccaaaggaat
     8221 aatcacgaaa ctagtgaatt tggatacttt gcgaatcgaa tctacaagtt cagaacgaat
     8281 agctttgtga gtctttgcct cgctagtcaa gcggacagca taattatctg cacctactcg
     8341 gataacttca gccatgacag aaccgcttgt caaaaagctt ccagaaagca attcagctcc
     8401 agtttctttg acaatcaaat cagattcgcc tgtcaaaaga gcttcatttg cttctgcccg
     8461 tccagatata acctgtaaat cggctggtat ctgttctcct gctccgattt ttaccaaatc
     8521 tcctaatacc agctctttag tagaaatcga ttgttcttta ccgtcccgga tcactagtac
     8581 ttctgattgg gaaacaatcg ataatttccg tacaagattt cttgcatgta tttcttgata
     8641 aattccaata acgatattga cgacaataat tgctaaatac gccatgttgg aatacgcacc
     8701 tacaaataac agacaaatcc caataccaaa gttcagaaaa ttgaataacg tcaatatatt
     8761 atcgctgaat atttgttttg tactttttga tgcatcttcc tggtagtcat tttgctggcc
     8821 agaagccatt ttctgaacta cctcttgctg tgtaagtcca ttcattttct ttttctccat
     8881 tttccgccaa ctttcaaaaa taatcacgtg tttaagtcta gcgtgttcca agattaaaaa
     8941 caacttgttt ttataaaatt tatagaaaaa cacacgaatg attttacaaa aaagatgcca
     9001 acctttttgt ttgaatcacc cgtgtgctgt ttttattatc aatctatctt tacttatatt
     9061 gttcctccaa acggctcatc caccaaccta gtgctgtacc ggctataaaa agaacaggac
     9121 aagctagtac cccagcaact cccaatcccg cgtaatcggt aggaatgatc ataatcgttg
     9181 aagcaaaaac tacacctaaa atgaaatgga atagcttgct atatgccttt gagaaaatgt
     9241 aatccatgat ttttgacaat ccaagaacac aaagaatccc accaattccg atcggcacaa
     9301 tcacttcaag tcgtacttct ttaaatccgt cagccatcgc tttatacaat cccatataca
     9361 ccaaaaaatt agaagggctc aatcctggga cgatcatacc caaaccaatc aatccgccgg
     9421 caatcagcca tgttccaaaa ttctgatcga cttgcccgtc aaataattga gcgccgtata
     9481 ataaaaacaa ataagccaga acaaaagtaa caaccatgat gatcacatca gaagtatctc
     9541 ttcctttttt ccctgcttct ttccataacg caggaacagt tccagcgata catccgacaa
     9601 aaaaccataa tatcgttgtt tcatacgcac ctaataaaaa actgactaaa aaagaaagta
     9661 gaaagatgcc tgtcactccg cctagaccaa caggcagaaa aaacaaaaca ttttctttga
     9721 aattttttgt aaggtgggct aaaaaagaaa tcagccgctc atacatgcca aagacagctg
     9781 ctaatgcacc accgctgact cctggcaaga taaatcctga accgataaac attcccttca
     9841 caaaccgtaa catccagtct ttggttgtgt accccacttc ttgattttcc attttttgcc
     9901 ttttttccac tatttgcgta ctccttcttt catttaattt tattcatgat tgaacatttc
     9961 ctgctatttt acttaaagac tttttcatag gtctctatta ttttttcatt cattttgaca
    10021 atatcaggat aaaacatgtt acctccatta tgatagagat agccgccatt gtaaagcaag
    10081 gactggatcc gccaatatcg gtattgtttc ccttcctcat ttcctaaaag tgggctcagc
    10141 acatctttag aatacgcttc tgctaattct actgtgtttt ctccgccatg attttttaca
    10201 taggcaatat aatctttccc aaaattatat gcttggacag ctgtccataa atcgcatcct
    10261 tgtttctttg cttctactat catttccgcc aaatatttga caccttgatc aatactgtca
    10321 gctggattat caatgatctc agcttctcca tgcagacttt ccgagctctg catgagatct
    10381 gtcccttttc cttttgattc ggtcaatatt atcgccttga tcaaattctc gtagttttcc
    10441 atatgataat tttttgcagc tgttgttatt tctatttgat agctttccac tcgttttacc
    10501 gaacgataag ttcttcctca taaaaacaac accgcaccaa taaccgtcag aatgatcatg
    10561 taaaaaagaa tacggataat agataatcgt tttcgcttca tttcatcacc tagttatttt
    10621 gacatctttt tttatcttac tatttcttta tcttcttggc tatttcttcc ttgatttctg
    10681 tgtacgcctt aagaaaaaat gaattttcct gtattttttg acatcttacg aaaatgatcg
    10741 ttgtttttca gtaaaaacaa ttgcccaatg actgatataa tcgttatact ttacacagta
    10801 ggtgattatg tgaaaaaaga aaagaaatat gcaaaaatgc cagatgggag cgaaatttac
    10861 tatgaaaaga gcggtcaagg cttccctctc tttttactgc atggcaatga cggcagcggc
    10921 cgcttttttt cagaacaggt ccctgtactc gaacgctact ataccgtgta tctagtagat
    10981 agccgaggac atggtcgttc gacaaacgaa gcatctatgt tgaattttca gttaatggcc
    11041 gaggatctga atacgatcat gttgctggaa aaaatcgatc aggcagattt tcttggattt
    11101 agtgatggag caaatctagc actggtattt gccagcagct tccctaaaaa agttcatcga
    11161 ttgattttaa attctggaaa cacgcttgtt aaaggcgttc gtttttctgc cagagtcatc
    11221 agtaatattc attatgcatg ggtatggtta ctctctttat ttcgcccaag tcttcgcaaa
    11281 aatcttttag ttatcaaact tttgctgcat gacatcgggc ttacagaaaa tgatttaaaa
    11341 aaaatcaact cccccacttt gatcatcgtg ggaaaaaaag atgtaatcaa gctaaaacat
    11401 tctttgtata tagctaaaac tataccaaaa gcttcctttg tgcttgtgaa agaacaagga
    11461 catgaattag ctagaaaaga tcctgaacgc ttcaatcgag aagttcttca atttctttct
    11521 gaaacataac tattaggaat agaaggtgat ttatttgtta aaaaatacca tacaatggct
    11581 gaaaaaccac ctaggactgt tcaaaacaat ctttttgatt tctgtcattg tcattattgt
    11641 cggtgaattg atgtccatcg gtaaaactct atctatccag caattaggag aaacgtttgc
    11701 cactatacct gtatggaaaa gcggactaat gctattgatt ggtcttatct ctgtgttacc
    11761 tatggttggt tatgatatta ttttgaatcg gatgcttgaa caaaagcaga atcctcgtta
    11821 cctgtttgag acaagctggc tgatcaatac gatcaacaat attgccggtt tcggcgggtt
    11881 tgttagtgtg gggcttcgct ctgaactata tggacaaaag aaagagagca gaaacgtgat
    11941 ccaagcatta tcgaaagttt tcttgttttt gatggctgga ctttctattt acagtctgct
    12001 ttctttttta cttgtagtct tcacgccagt tgctcctttt ttgaagcaat attggatatg
    12061 gttgatcggc ggcggtctat attttcctgt cgtcttttta tttactactt tcaaaaagaa
    12121 aggattcctt ggtggtacct cacttaaaac tcaaggacag ctattgctag tttcattgtt
    12181 ggaatggacc ggtgtcctgt ttagtttcct ttctgttggt tatttaatgg aaatccggat
    12241 tgatctgtgg caaacaattc cattattcat tgctgcttca gtgataggaa tcgtgtctat
    12301 gattccagga gaaattggga gtttcgatgt aatgatgatt atcgggttgt ccgctattgg
    12361 tgtgccaaga gaaatagtcg ttgtgtggat cttgctctat cgcctgttct actacatcat
    12421 tccgtttttg attggaatcg tctttttctt caaaaatatc ggttccactt ttgatcagcg
    12481 ttattctggt atccctaaac aattagcaac agaaattgcg cataaaatcg tggttgtctt
    12541 attatacttt tctggaatca tgctagtact ttctgccact atcccgcaag cttttacaga
    12601 gtttcgctgg cttcatagtt tgaacccatt gaaatttcat tttattatcc aatttcctag
    12661 tatactattg ggatttcttt taatcattat gggcagaggg atcgcagcac gtgtgaaacg
    12721 agcatatttg cctacaatct tcttgattgt tctagcttta ctttatgttt tattaagtga
    12781 ttttagtttc acaccggtgg ttttcttgtc tattctgctt ctgatcattc ttgcttctaa
    12841 aaacgaatta tttcgggaac aattgatcta ttcatgggag tggcgtacga tcgatggaat
    12901 cattattggt gcactgtcct tgctttacat cgttattggg gtatacaatc tgccggattt
    12961 ccctcatcgg cgtcaccatt ttatttcctt tttcttattt ccttctgaaa agatttggtt
    13021 ttcgggattg ttggccatca tcgctgttag tttcatgatc gttctatttg ttcatttcct
    13081 tcaaggagaa aagaaacaga ttggcgaagc ctttaatgaa gaaaaggccc tgaagatcct
    13141 tacaacttat ggaggaaact ccgatagtca attgatcttc ttaaaagata agcggatgtt
    13201 tgcctatgaa aaagacggtg aaccaactgt tcttctccaa ttcgcttgct tcaataataa
    13261 atgtattgta atgggtgatc cttctggtaa gaaagaagat tttccagaag caatcgaagc
    13321 ttttatcgaa gaaacagatc gactatgcta tttaccagtt ttctacgaaa caagcgaaga
    13381 gattgttatg attttgcatg aattcggcta cgatttcatt aagatggggg aagaagctta
    13441 tgtagacctg aatagtttta caacttcagg gaaaaaaatg aagggcactc gtgctgtctt
    13501 gaatcggatc gaacgagaag gatttacatt tgatgtctta caaccaccat tttctactga
    13561 gcaaatgagt actttcaaaa atatctctga taattggttg ggttcacgaa aggaaaaagg
    13621 gttttcttta ggctttttct ctgaagatta tctacaaaga gcgcctattg cagttgtcaa
    13681 aaatacccaa gaagaaattg tcgcttttgc aacgatcatg cctacttaca cgaacaataa
    13741 agtaggaacg attgatctga tgcgatatga ttctaaaaaa gcaccttctg gcagtatgga
    13801 tttcttgttc ttgcatttgt ttgcctatat gaaagaagaa catatccaat ggttcaattt
    13861 agggatggcc cctctttcaa atgtcggaac ttccagaaag agttttttac aggaaaggat
    13921 tgcatacttg gtttacgagt ttggttctca tttttattct ttccatggat taagagaata
    13981 taaaagcaag tacgctacaa actgggtctc acgttatact ctatattcta gagatagttg
    14041 gattgcatat gttatgattg ctttgctgat tattgataat gcgccagttg agcagacaca
    14101 aggtagaata agtggtttga aaaaatggat acgaagaaag tattaaaaac tgcataccga
    14161 tagatgaaca ctctcatgat gaataaaaga gactgaagta aaacgaaagt tcatgcttct
    14221 acaatctctt cttatacaat tgaagaacaa agagcggtcc aaacagaaat cctgtttgga
    14281 ccgctctttt attttaaaca tttttcatta ccaaaatatt cgagtcgcgt ttcttatgct
    14341 tcgatctctg taacgatacc tgaacctact gtccgtccgc cttcacgaat tgagaatgtc
    14401 gttccgtttt caacagcgat tggatggatc aagtcaacat caatcgtcac gttgtcacca
    14461 ggcatgacca tttctgtatc ttctggcaat gtgattgttc ctgtaacatc agttgtgcgg
    14521 aagtagaact gtggacgata gttgttgaag aatggagtat gacgtccacc ttcttctttt
    14581 gtcaacacgt acacttctgc tttgaatttt gtatgtggag taatggaacc tggtttagca
    14641 agtacttgtc cacgttcgat atcgtcacgt tggatccccc gtaacaacac gcctacgtta
    14701 tccccagctt ccccgtaatc taacgtttta cggaacattt ctacacctgt tacaactgct
    14761 ttttgtgttt caggtttgat ccctacgatc tctacctcat caccgacacg aacagcacca
    14821 cgatcaatac gacctgaggc aacagttcct cgacctgtaa ttgagaagac gtcttccact
    14881 ggcaatagta atggtttatc tgtatcacgt tctggtgttg ggatatattc atctactgta
    14941 tccatcaatt ccataatagc agcttcagca tctggatcgc cttgcaatgc ttttaatgcg
    15001 gaacctttga tcacaggagt gtcatcgcct gggaaaccat attcgctcaa taactcgcga
    15061 acttccattt ctaccaaatc gatcaattct tcatcatcga ctaaatcaac tttgttcaag
    15121 aaaacaatca ggtattttac accaacttgg cgtgacaata aaatgtgttc acgtgtttga
    15181 ggcatcggac cgtcagttgc agaaacaact aagattgcac catccatttg tgctgcccct
    15241 gtaatcatgt ttttcacata gtccgcgtgt cctggtgcat caatatgcgc gtagtggcgt
    15301 ttttctgttt catattctac gtgtgcggta ttgatcgtaa tcccacgttc acgttcttct
    15361 ggagctgcat caatactagc atagtcttga ggatttgcta ggcctttttt gcctaatact
    15421 gtagtgatcg cagcagttaa agtagttttc ccatggtcaa cgtgtccaat cgtaccaatg
    15481 ttaacgtgtg gtttacttct gtcataatgt tcttttgcca taatttacaa acctcctaaa
    15541 taatatagat cactgaaaaa tttcagctct caccttttta ttatagtctt tttttgacta
    15601 aatcaaaaag aatacgtccg acttactttt tgtaagattc gaactaagct tattatacgt
    15661 caatattggt caaagtcaac taagagcact cgtctgtctt atacgcgaaa aaagtacctt
    15721 ttataaataa aaaaatgtgt tttttgagaa aaacttcaag atagtcctta actttctttt
    15781 gaagcaataa gaacaaaatg aggaaaagtt taaaattcag ctgttgccaa caactttcaa
    15841 tagacgaaat cttctatttc gttaataata aggaaagagg tgaagtacat gaaaaaatta
    15901 attggcgtac ttgtcgtttt aggcatattt ctcggcggaa gttttgcagc ttatcattat
    15961 ttttacggcg gcgaagctta ttatacaaaa atcacgacga atggcgaaat gtcagctacc
    16021 caagcagata acggagagaa attcacgact tatacctaca agcaagcggc ttataataaa
    16081 gatggtgaaa aaaaacaggt gacgcttcgc gaagaaagag aacatccatt aaagatgaat
    16141 gcgtatctta aactcaaagt caatcctcgt aaaggtgtat taagctggga agaaataaag
    16201 gcttctgaag tgcctaaaaa tgcggcagaa aaaatcgata attagtaaat ggtcttaaaa
    16261 ttttattaga taaaa
//
LOCUS       DAZKMB010000059        16062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000059 DAZKMB010000000
VERSION     DAZKMB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.059"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..684)
                     /locus_tag="ACG8V3_001693"
     CDS             complement(37..684)
                     /locus_tag="ACG8V3_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0300230.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(692..1378)
                     /locus_tag="ACG8V3_001694"
     CDS             complement(692..1378)
                     /locus_tag="ACG8V3_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300231.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1384..2457)
                     /locus_tag="ACG8V3_001695"
     CDS             complement(1384..2457)
                     /locus_tag="ACG8V3_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0300232.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            2679..3353
                     /locus_tag="ACG8V3_001696"
     CDS             2679..3353
                     /locus_tag="ACG8V3_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0300233.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3358..4698
                     /locus_tag="ACG8V3_001697"
     CDS             3358..4698
                     /locus_tag="ACG8V3_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0300234.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(4847..5833)
                     /locus_tag="ACG8V3_001698"
     CDS             complement(4847..5833)
                     /locus_tag="ACG8V3_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0300235.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            6557..7588
                     /locus_tag="ACG8V3_001699"
     CDS             6557..7588
                     /locus_tag="ACG8V3_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0300236.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            7697..9229
                     /locus_tag="ACG8V3_001700"
     CDS             7697..9229
                     /locus_tag="ACG8V3_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0300237.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            9368..10180
                     /locus_tag="ACG8V3_001701"
     CDS             9368..10180
                     /locus_tag="ACG8V3_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0300238.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            10170..10655
                     /locus_tag="ACG8V3_001702"
     CDS             10170..10655
                     /locus_tag="ACG8V3_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0300239.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            10678..11223
                     /locus_tag="ACG8V3_001703"
     CDS             10678..11223
                     /locus_tag="ACG8V3_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0300240.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(11296..12939)
                     /locus_tag="ACG8V3_001704"
     CDS             complement(11296..12939)
                     /locus_tag="ACG8V3_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0300241.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(13068..15296)
                     /locus_tag="ACG8V3_001705"
     CDS             complement(13068..15296)
                     /locus_tag="ACG8V3_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0300242.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(15329..15808)
                     /locus_tag="ACG8V3_001706"
     CDS             complement(15329..15808)
                     /locus_tag="ACG8V3_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0300243.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT         4741 a         3062 c         2907 g         5352 t
ORIGIN      
        1 aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca
       61 acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag
      121 atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc
      181 cgacgattga atggagaaat gattgattct taataattcc agccattttt cgttcattgg
      241 agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa
      301 gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt
      361 ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg
      421 gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata
      481 tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg
      541 gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc
      601 gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat
      661 acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca
      721 agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag
      781 taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt
      841 catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct
      901 cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag
      961 gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg
     1021 aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa
     1081 aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc
     1141 gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg
     1201 gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa
     1261 tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct
     1321 ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt
     1381 ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa
     1441 tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa
     1501 aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc
     1561 aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa
     1621 gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat
     1681 cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc
     1741 atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt
     1801 taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac
     1861 gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc
     1921 agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa
     1981 gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt
     2041 atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg
     2101 ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt
     2161 ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc
     2221 ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc
     2281 ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga
     2341 aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc
     2401 gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg
     2461 tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc
     2521 taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc
     2581 tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc
     2641 atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag
     2701 aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa
     2761 cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg
     2821 caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga
     2881 aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga
     2941 ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac
     3001 tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc
     3061 tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag
     3121 ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa
     3181 tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga
     3241 ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg
     3301 ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg
     3361 aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt
     3421 gcttttatct tttttctttt gggattcatt actttacaga tattaaacca atctgcatac
     3481 agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag
     3541 atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga
     3601 acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc
     3661 ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa
     3721 atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat
     3781 gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat
     3841 caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta
     3901 gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt
     3961 atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa
     4021 aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat
     4081 agtttggaac atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc
     4141 gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc
     4201 gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat
     4261 atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt
     4321 ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg
     4381 gttattttgc tggataatgc attaaaatat acgaatccag aagataagat cattttggat
     4441 tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt
     4501 gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa
     4561 gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat
     4621 ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg
     4681 ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca
     4741 gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
     4801 atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat
     4861 tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat
     4921 cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa
     4981 taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat
     5041 cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga
     5101 cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt
     5161 ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta
     5221 gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac
     5281 ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg
     5341 gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc
     5401 cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg
     5461 tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag
     5521 aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat
     5581 aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat
     5641 ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag
     5701 atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca
     5761 accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat
     5821 ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat attgttactt
     5881 ataaatttat tctataggag acgattgttt tctacaaata tttagattgg aatttgatta
     5941 aaacaattta attgaaattt gaaaacattt atgttaatga aagtcatatt ctaataaaat
     6001 gtcatttgtt tctatatgaa tttttatgaa aaatttcata taatttccac tcgttgtgat
     6061 ggtttaattt atccaatcca tcaataagca aacaacttat ataaaaaagg attaattctc
     6121 aatataattg ctcctaccat agaataaatc ttcatgtaaa gtaatgaagt accaactatt
     6181 tctaatactt ctttaagaat tatcccaaca atatcgttag acaaaccatt attgaataaa
     6241 tggagagcca atcgattctg aaaaaatgtt ttctaatttt tcggaaataa aagaaagagc
     6301 ctcctgccat tagaacgaac caaatccatt tcattgctca tgaagtaacc acatagctaa
     6361 aaagaggata aatcaattaa aagatcaaaa gaatgactgc actaataaaa taactaaaaa
     6421 tgtatcttct tacttcatat cttttagcta gtacttagtt aaaaatttgc ttcctaagat
     6481 caagacaaac tgtctatctt catttgatga aatgcggtaa aatatagaaa atgaacacta
     6541 ttggagggaa tttgtaatgg acagcaaaac tagaaaacaa ctgcaagaaa agttaaagca
     6601 acgtacacac ttgctccctt ccgtctttca acagttttta gaagaaaatc gttcttccct
     6661 ttcattaggc agtcgctatg aatacgcaaa agattttcat ctgtttactg attatttgag
     6721 aaaagtattt tctgaagaag tggatgatca gctgattatc catttagaaa aacaagatta
     6781 tcagaatttc ttagctaaaa tctatcgaca tactcgttcg ttccaaacta cagcgaacca
     6841 agaaacggag caattgtttg aaaatagcta ttacgggatt tcaaggaagc aatattcatt
     6901 aaatcatttt ttacgatttt tgtatcaaaa aggactgatt gaagaagaga tttctctttt
     6961 gggtacaagt ttaccggaaa caaccaaagc agctccgcga tatatcgatg cagaactttt
     7021 caaagatttt gagcatttgt ttatcccgat tgaaggcttt caaacaagca gagaagctag
     7081 ctttgaagaa aagaatagac cgaggaacct tgcaattact gccattcttc tgtattgcgg
     7141 attaaagatc catgaagtag tagaacttaa acgtaaagat gtatctttga ccaagcagat
     7201 tcttaatctg aatagaaaag aaaacagact gaataaagta ccaataccaa aagaagcgat
     7261 gccatttctg gagaactatt ttaagttaac agcacaaaat acggacgaaa attcttttgt
     7321 ttttcgttcc tcacatgatc agcaaatttc tcctcggact attcgccaaa ttttgagcaa
     7381 aattacagta tataaaaacg tatcgccaga actatgcaga aaaacatatc gttattatgc
     7441 agaactatat ttagatgatg ctgacgcggt aaattattta tgtggtaatc gatacctctc
     7501 atcccattct tttcaagata tctgggaaaa gatggttgat ttttcttatc acgaattagc
     7561 ggcattcgtt cgggtttctt ccatataaaa caaatgtatg atcttttttt cacaataaac
     7621 tatgttatta tataaaggat tatgttacga ttatcttgaa tttgtatatt acttaacaaa
     7681 agataatgga gtgtgaatga tggagaccat aacgtattcc cctttgaact tatttacgaa
     7741 ttttcaagaa gctgcagcta acttttccga gaccccgatt atctttgatg agccactttc
     7801 agctttccca gaattaggac ttgaaacaac ttacaaaaaa tgctgcgaaa gtgtgttgaa
     7861 acgggcttat caattggctc atcttggagt gaaatccgga gacaaagtta ttatttataa
     7921 gagttccgct tttgacacat accttctagc tgtttctgtg tcttaccttg gtgcagtgcc
     7981 tgtcatgact tcttatcatc taccaacaac aacaatggaa gtatttatcg atcgtttaga
     8041 agaccctttc attttatttg atgatgagac aaaaaagcga gtgagagaaa tttcaaatgg
     8101 aacaaaatca aaacagattc ctattcttta tttaatggaa caacctgctt tgtctgtttc
     8161 tcaatcattt ttagacaaaa atgaaatttc ttatatgacc catacttctg gtacaactgg
     8221 tatccctaaa cttatttgtc attctgctca ttctatggga tggcggacga aatggcaaaa
     8281 aacagttttc acaaaaattt cggaaaagaa attaatcgct tttcacatct ctccagtgca
     8341 ttccagattc aatattggta tctcttcttt gatgtccatg ggatttccta tgatgccttt
     8401 agcaaacgca caaagttcaa aagttgttca tatgttggaa gctcaccggc caattgcact
     8461 agagacccat ccgaataact ttgttcagtg gcgttttact gctaaggagc atccagaagc
     8521 tttttcgggt atccgatact atcactccac ttttgatgct ataaacaacc agacaatgaa
     8581 gacatttttg cgaacgtcga tggcacagga tgcggtattt cttcaagtat atggccaaag
     8641 tgaatgcgga ccaatgattc ttaaggctca tacactagag agtttgaaaa caagtgatgc
     8701 aagagatatg ggcgttggac tggaagacat gacaagcgct cggattacag attcagttgg
     8761 gaatgtccta cctgaaaaca cagatggtca tatacaatta ctgtctaaag gacgggcttt
     8821 gacttattac aaggaagatg cacggttcca ggaaaatgtt tatggcgatt ggtgggatag
     8881 cggagattac ggttttatgg ataagcatgg ccacctattt ttgaaagatc ggcaagtgga
     8941 tttgatcgaa acgatcaata gtacattagc tattgaagac tttttattag attcgttaga
     9001 ttttttggca gaagtagtga ttgtaaggga taaaaatcat tctccacaac cgattattgc
     9061 tttagctcct gataaagaaa tggattggaa tcgatggtgg gagcaagtcc atgaacttcc
     9121 tcgcttgaac gtccctatca tccgtgattt tgatgcaatc ccgcgaacag ctacaatgaa
     9181 agtgcaacgg cttcaaatcg agaaagaatt aaaatcacaa aagtcctaac tatttaagga
     9241 aagaaagatc cattttggtc tttcttttct ttttgttttt ttaaattgga tgagataaaa
     9301 atatgccaat tggctgtttt tcttttttga aaatttcgtt atactcttat tcatggagga
     9361 aaatgttatg aaaagaatct tgacaattgg cggttctgat ccatttgctg gtggaggaat
     9421 ccaaacagat ttaaaaactt ttgagaattt tggtctattc ggactaagcg ccttgacttc
     9481 tataggggca ctagatatta atgagcgatt tatgctggaa tctatatctt tggaattgct
     9541 taaacgacag ttagtttcga ttgataaaat gacttcatta gatggtatca aaattgggtt
     9601 attgaattca agtgaagcaa tcatggttgt tcgagacttc ataaaaacaa aacaaaatat
     9661 cccaatcatt ttagaccctg ttttggcatt caaagaaact gaagctgtga tcaatcaatt
     9721 gtatatttcg cagctcatcg aagaactttt tccacttgtt acgatgatta cgcctaattt
     9781 ggcagaagct gctttacttt ctggtcaagc tcttccctct tcgttaacag aaatgatcca
     9841 tctcgctaaa gaactcactg cttcaggaac aaaaaacatt gtgatcaaag gcggcacaag
     9901 gatggctgga gatgaagctg tggatcttct ctattcaatt gatcattatg aggtattttc
     9961 tcataaaaaa atcgacacta caacagtaaa cggggccggt tgtgcctttt cttctgcaat
    10021 aacagcgaat cttgtgttag gggcagattt taccaaagca gtagcagaaa gtaaaaaatt
    10081 tgttgcccat tgtatttcta atggcgtgct gatgaacgat cataccggaa gtgtctggtc
    10141 tggcggtatt caaaaaggag gaatgagaaa tgaaaactaa gcaattaacg atctatgcta
    10201 tgctgattgc gttgacagtt gccctttcat taacaatttt gatccctgta ccggctacga
    10261 atggatttgt gacattatgc gaggccggga tctataccgc tgcttttctt tatggtccaa
    10321 cgggtggatt agttgttggt gcttccagtg gtctattgat cgatttgatt tcaggatatc
    10381 cgcagtgggc agttttttca ttagtgatcc acgggttaca aggatatgtt gctggttctt
    10441 tcagcaaatc ttctactcgt atgtggggat tagggctttt attaggaagc ttagttatga
    10501 tcatcggcta tggtatcgcc ggttggattt tatatgattg gccggctgga gtggcttcta
    10561 taccaggaaa catcttccaa aatctgattg gaatgggact tgcctttcct ataactgcaa
    10621 gtcttcaacg tattcaagca cggaagcaat attaattgaa aaagaaggtt ttagaaaatg
    10681 aagttgtcag aaaaagaatt aaaagaccaa ttgactgaaa tagttaatga catattggct
    10741 gaggcacact tgaaaaaagg tgatcttttt gttttaggat gcagcacaag cgaagtcgtt
    10801 ggaggacata ttggaaaaaa ttccagtgcg gaagttggtc aatggatcat ccgtacgtta
    10861 aaagagttgt tggatcctaa agaaattgcg ctagctgtcc aaggttgtga acatttgaac
    10921 cgagcattag tagtagaacg aacagtggca gaagcaaaaa atttcgaaat tgtctctgtt
    10981 gttcctgcac tacatgctgg aggtgcttgt tctgttgctg cttttgacca gttcaatgat
    11041 cctgtcgaag tggaacacgt agttggacaa gccggtatcg atatcggtga cacatcaatc
    11101 ggtatgcata tcaagcatgt ccaagtacct gtcagacctc gtttgaaaac ccttggacaa
    11161 gctcacgtga cagcccttcg ctctcgtcct aaatatatcg gtggtcctag agcgaattat
    11221 tgaaatgagt aattgacaga gcaatctgaa aaacttcctc gcaaagtcca actaatgaac
    11281 tttaacgagg aagttttact tgtttgtttt atataaccga gcttcataag gtgctagtat
    11341 gcctttagct tcaaattctc cgctagagga atttgacaag agtaatgtcc aggactgatc
    11401 tgcttctttt ggaagttcat agtcagcaag tgattctgtt aagtttacca taataagata
    11461 ttgttcatct tctaaatatc tttcatagac ataaagttgt ggatgttcat gaagatattc
    11521 tttataagaa ccgtatatca gacctttatt ggtttgtcta atgcggatca tgttcttata
    11581 gaattgcagg accgactctg gttcatattc ttgttgttct acattgatcg ttttggtatt
    11641 tggattagtg atcagccaag gttcatgtgt agaaaaacca gcatattgtt ccgaagtcca
    11701 ttgcatgggt gtcctgctgt tgtctctcgt tgtttcacga atgatatcca atgcttcttc
    11761 tcttgtctta ccttccgcaa gcagcctttt ataaagtctt ttcgaatcta ctgcatcaac
    11821 ttgatcgatc gattcaaagg gcatattcgt cattccgatt tcctgtcctt gataaataaa
    11881 tggcgtacct tgcaaaaaga aatacatcat agcgattgct tttgcagaca tagcccaaaa
    11941 atcgggacga gtatctccaa acactgaaac ggcacgagga acatcatggt tttccatata
    12001 taaagcgttc catccgatac catccaatga agtttgccaa gcgcttaatg catgtttcaa
    12061 tttaaggaca tctaattgcc cttctttttc ttgctgccag atatggatat gatcaaattc
    12121 gaatatcata tcgaaataac cgtctttccc tacccattgt ggtccttctt ctgcagtgac
    12181 accacttgct tctccgacag tcatgatatc gtactcttca aaaacatgtt tcaaatcggt
    12241 caaatgttct tcgattcctg agacattttg aaagggtgag aaagggttct ctgtggcttt
    12301 tactgaatat tcatcttttt ttacatgaga aatcgcatct aaacgaaatc catctatccc
    12361 tttatccaac caccatcgga tcatattgaa tagttcttct ttcaatttct cgctttccca
    12421 atttaagtct ggttgttctt ttgcaaaaac atgcagataa tactgtccag tttcatgaga
    12481 atactcccaa gcagaaccgc cgaaaatcga tgtccaatta ttcggttctt tgccatctac
    12541 cccgtcgacc caaatgtaat aatcgcgata cgggctttct ttagattttt tcgattcttc
    12601 aaaccattga tgctgatccg aactatggtt gatgacaaga tccataatca ctttgatccc
    12661 taattcatgt gcttctttta gcaataaatc aaaatcttcc atcgttccga atctctcttc
    12721 tatatcttga tagtcactta cgtcataacc attatcgaca aatggagatt gatagatcgg
    12781 attgatccag ataaagccga taccaagatc tttcaaataa ttaagttttt cacggatccc
    12841 attgatgtct ccttgaccat cgccattgct atctttgaaa ctagcaggat agatttgata
    12901 gcctactgct tctttccacc atttttgttc ttgtatcata tcgtttcact cctttaacgg
    12961 tccctcttga ttataaccga ttctatttta gaagtatcct tgttaccggt aactagaaac
    13021 gaaaagctca acgaatctgc taagtgcgtt cgttgagctt tattttatca tagatagcgg
    13081 gcaatcagtt gaatcatttg cttgggaaat tcattcaagt cagtaatatc tacaaaacga
    13141 ctttcaccgt agatcgactt gatttcttct ttgtcttggc caatagctgc ggagacaaat
    13201 aggattcctt gacgttcata ttctttcaaa acagactgga tatcttcttt tgcttttttg
    13261 cctgtataat ccggcaacgc ttttggctga ccgtcactaa tattaagcaa cagtttggtt
    13321 gttgcggatt gtctgtttaa tttttctgct gctaaacgaa gtgcagcacc atcacgattg
    13381 ttttgccttg gtttcatagt gactaatttt tcatttaacc attgaaatcc gtcttcaaat
    13441 tctttataag aaaacaaaga agtcttttct cttgacgaac ggtctgctgt atctccgtaa
    13501 atcaacaatg ggatatcgac tttcttagaa aattcggcaa tggcaatcgc tgcttttttt
    13561 gccgcttcga tccgatcatc ccgtatcatt gacccagatt catcgatccg tacagctacc
    13621 gctaaagaag gttgctcatg tggcgggttc tttttgtcaa aatttctcaa atcaccatat
    13681 gctactttgc ttgcattgaa ccgctgacct tcgtattttc ctttttggta cgtctctgtt
    13741 tgttcattat ccagtaactc taaaatttgc cggctaagtg attctactat tggcattact
    13801 tgcatgtgta gctcacgtgc ttccggcgtt tttccttgga aagctggtcg gtgaacgatg
    13861 agaccttctt tttcatgaat cgtttccttc atgatttgtc gcgcttcttt attcagttgc
    13921 ttcttgattt ccttatcatg tgcttccatg tcagaagcag acatttcttg agaatcatct
    13981 cctgtttgtt gttctttttg attttttcct tcgatatcgc tactgcgttc tttttgcatt
    14041 tgaggaggaa tctcgttttt ttctattgtt tcttcttgtt tctcttctcc ctcatttggt
    14101 tcttcagcag acaacgattt cttttcatct aagcgcagaa tttcttcagc attttcagtg
    14161 atgtttcgtt catttgcttc ttcggcgcta atggttcttt cttttgtatg ctcagattga
    14221 ggatgacccc atttttcttt tctcaatatg tcctgaagtt ccagtagcaa tccactgtca
    14281 gacaaagctt gttcaagaat tgccaattca tctgaatcag tggataattt atacaaaact
    14341 tttggataaa tcgactggag taaatcttgt ccttgcttat aggtatttgc ccaataaaaa
    14401 taagaacgca ttcctgctac acctttgatg gcgttagctt ttgcagtttc atccaacaaa
    14461 cggataatct ctgccatctt taagagaagt tcttgttttt ggatcatagt tttcgccatc
    14521 gcacgttctg ccatgacttc taaggcagga agatccattt tttccgcatg ttgcatccga
    14581 tctcttaaag attcatttaa tgggcgattt ccttggtaat ttcgatttgt agtgatcaca
    14641 atgacgcaat ctggatggcg acgaatgatt cctgtcggta aattcaacat cccatttggt
    14701 tcgagcgctg agttaagtgc cacaagaaca gaggcatctc ggatgacagt cggctcttga
    14761 atctctaata aatagccttt ttcaatcgct cgaacgattt cagaagggta atagctgtaa
    14821 tggattgcat ctggataatc agcttcgatc ctttgaacta gctctgctaa tttttcttta
    14881 gcagaaaaag gatcaatgcc gtaatggttc tggatcagtg tcagtacagc ttcaagtgta
    14941 tctgtttggt aaatagcttc tagtaattct tggtcttgtt ccttattaga atctactaca
    15001 ggtaataagg ctccgaaaac atctgatttg tccatatctg caaaacaggt cacttttgta
    15061 tagggaagtt gcagatcagc agatagtgct ttggctaatt gtgtcttccc cgacccggca
    15121 tctccttcca aaagtacgtt actgattttc atttcaggat cttgccaatt ccctttgatt
    15181 tcggaggcga tccgccattc ttctgcactt gtccgatggc ttttcggttt cttccagatc
    15241 atttgctttt ctagccgtga aaaggttcgg ttttctgtta gtgagaaatt ctccatgctt
    15301 ctcttcccct tttgatcatc tctgtctatt aatatcctag ctcatctaaa agacgtttta
    15361 aaatacggaa tcgttcccca atcagttctc cgtctttttg tagtgtatcg taatacaaaa
    15421 tgatgtcttc atcgatcttt ggatttttgg tatctacttc taccgtcatt cggttccctt
    15481 gtaatccaat gatgtctacg actggatttt ctggatcata aaatttttca taacgataag
    15541 catcttggaa gtataaactt ccttgcgcag ctaagatcgc taaatctaaa atacgtgtga
    15601 ttggtaattc ttccgactgt cgtgaccatt tttctccagt atgtcgccat accttgcctg
    15661 agatatcgac ttttccacga tcattccact gagcaaggcc cagtgaaagc ccttttgcat
    15721 ccatatttaa tgcatttctt ccatcaactt tatcatagtc ttctacaacg attactggtt
    15781 tatgttttaa atttgttggt atttccatta ttttaactcc tcatcctttt atttttagta
    15841 aataagtaaa ttagtagttt actcatttac taaactatat gcgacattat agaggattga
    15901 agtgaggatt gcaaagaaaa agagcttcat tgatagaaat aaaaacacca tctcgttctt
    15961 ttacacgctc attagtaaca tgtagaaagt ccaagatgat gttggaatat tttggctcta
    16021 tactttttga tgttggtgaa cttcaccaac atcttttttg ta
//
LOCUS       DAZKMB010000060        15995 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000060 DAZKMB010000000
VERSION     DAZKMB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.060"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG8V3_001707"
     CDS             226..894
                     /locus_tag="ACG8V3_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0300244.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG8V3_001708"
     CDS             complement(1094..1414)
                     /locus_tag="ACG8V3_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300245.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG8V3_001709"
     CDS             complement(1451..2833)
                     /locus_tag="ACG8V3_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0300246.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG8V3_001710"
     CDS             complement(2989..4176)
                     /locus_tag="ACG8V3_001710"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0300247.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG8V3_001711"
     CDS             complement(4183..5214)
                     /locus_tag="ACG8V3_001711"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0300248.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACG8V3_001712"
     CDS             complement(5230..6684)
                     /locus_tag="ACG8V3_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0300249.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="ACG8V3_001713"
     CDS             6859..7764
                     /locus_tag="ACG8V3_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0300250.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG8V3_001714"
     CDS             7855..8532
                     /locus_tag="ACG8V3_001714"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0300251.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG8V3_001715"
     CDS             8529..8699
                     /locus_tag="ACG8V3_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300252.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG8V3_001716"
     CDS             8813..9709
                     /locus_tag="ACG8V3_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0300253.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG8V3_001717"
     CDS             complement(9764..11134)
                     /locus_tag="ACG8V3_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0300254.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG8V3_001718"
     CDS             11372..12118
                     /locus_tag="ACG8V3_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300255.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG8V3_001719"
     CDS             12115..12954
                     /locus_tag="ACG8V3_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0300256.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACG8V3_001720"
     CDS             12955..13905
                     /locus_tag="ACG8V3_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0300257.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACG8V3_001721"
     CDS             14046..14798
                     /locus_tag="ACG8V3_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300258.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACG8V3_001722"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACG8V3_001722"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0300259.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5190 a         2997 c         3222 g         4586 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agttt
//
LOCUS       DAZKMB010000061        15743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000061 DAZKMB010000000
VERSION     DAZKMB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.061"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG8V3_001723"
     CDS             complement(24..722)
                     /locus_tag="ACG8V3_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0300260.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG8V3_001724"
     CDS             complement(715..2067)
                     /locus_tag="ACG8V3_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0300261.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG8V3_001725"
     CDS             complement(2290..3690)
                     /locus_tag="ACG8V3_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0300262.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG8V3_001726"
     CDS             3851..4588
                     /locus_tag="ACG8V3_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0300263.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG8V3_001727"
     CDS             4713..5192
                     /locus_tag="ACG8V3_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0300264.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG8V3_001728"
     CDS             5189..5956
                     /locus_tag="ACG8V3_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0300265.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8V3_001729"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8V3_001729"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0300266.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG8V3_001730"
     CDS             complement(6859..7461)
                     /locus_tag="ACG8V3_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0300267.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG8V3_001731"
     CDS             7567..7797
                     /locus_tag="ACG8V3_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300268.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG8V3_001732"
     CDS             7788..8036
                     /locus_tag="ACG8V3_001732"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0300269.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG8V3_001733"
     CDS             8142..8726
                     /locus_tag="ACG8V3_001733"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0300270.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8V3_001734"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8V3_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0300271.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8V3_001735"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8V3_001735"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0300272.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG8V3_001736"
     CDS             10627..10806
                     /locus_tag="ACG8V3_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300273.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG8V3_001737"
     CDS             10828..11853
                     /locus_tag="ACG8V3_001737"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0300274.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8V3_001738"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8V3_001738"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0300275.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACG8V3_001739"
     CDS             13425..14372
                     /locus_tag="ACG8V3_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0300276.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACG8V3_001740"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACG8V3_001740"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0300277.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACG8V3_001741"
     CDS             complement(15274..15516)
                     /locus_tag="ACG8V3_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300278.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACG8V3_001742"
     tRNA            15637..15709
                     /locus_tag="ACG8V3_001742"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5350 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tta
//
LOCUS       DAZKMB010000062        15667 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000062 DAZKMB010000000
VERSION     DAZKMB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.062"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..749)
                     /locus_tag="ACG8V3_001743"
     CDS             complement(36..749)
                     /locus_tag="ACG8V3_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0300279.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            924..2348
                     /locus_tag="ACG8V3_001744"
     CDS             924..2348
                     /locus_tag="ACG8V3_001744"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0300280.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2647..3354
                     /locus_tag="ACG8V3_001745"
     CDS             2647..3354
                     /locus_tag="ACG8V3_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0300281.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3341..3664
                     /locus_tag="ACG8V3_001746"
     CDS             3341..3664
                     /locus_tag="ACG8V3_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0300282.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3753..4790)
                     /locus_tag="ACG8V3_001747"
     CDS             complement(3753..4790)
                     /locus_tag="ACG8V3_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0300283.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5111..6094)
                     /locus_tag="ACG8V3_001748"
     CDS             complement(5111..6094)
                     /locus_tag="ACG8V3_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0300284.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6509..7279)
                     /locus_tag="ACG8V3_001749"
     CDS             complement(6509..7279)
                     /locus_tag="ACG8V3_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0300285.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7272..8207)
                     /locus_tag="ACG8V3_001750"
     CDS             complement(7272..8207)
                     /locus_tag="ACG8V3_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300286.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8523..10172)
                     /locus_tag="ACG8V3_001751"
     CDS             complement(8523..10172)
                     /locus_tag="ACG8V3_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0300287.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10289..12892)
                     /locus_tag="ACG8V3_001752"
     CDS             complement(10289..12892)
                     /locus_tag="ACG8V3_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0300288.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12904..13953)
                     /locus_tag="ACG8V3_001753"
     CDS             complement(12904..13953)
                     /locus_tag="ACG8V3_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0300289.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13979..14293)
                     /locus_tag="ACG8V3_001754"
     CDS             complement(13979..14293)
                     /locus_tag="ACG8V3_001754"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0300290.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14309..14755)
                     /locus_tag="ACG8V3_001755"
     CDS             complement(14309..14755)
                     /locus_tag="ACG8V3_001755"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0300291.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14756..>15667)
                     /locus_tag="ACG8V3_001756"
     CDS             complement(14756..>15667)
                     /locus_tag="ACG8V3_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0300292.1"
                     /translation="AAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCI
                     HFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIED
                     VLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDA
                     FLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMND
                     FAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEI
                     ASNHELLANLKTSSSFEKLVYILKEGL"
BASE COUNT         4649 a         3222 c         2605 g         5191 t
ORIGIN      
        1 aaaaaataag ctctgaaaaa ataattttcc cgagcttatt tttcaacaaa aacagcttca
       61 aaaacaaagt cttgatataa atgtctcgat accgaatact cgaaaggttt accagacgat
      121 agagcaacaa ctttgtctat ctcaatcata aattcacctg gatctatttt taatattttt
      181 gcgtcaaatt cattcgcagt atctcctttg atacgtatgt gcgaagtctt tatttccaga
      241 cccagttcat ttgtgatata agcatataca gatttcttta aatgttttgg ttccaagcct
      301 ggtatgactg ctaatggcat gaatgtttgc tcaatcgagt atggttgaca atcaataaaa
      361 cggcttcgta caatattata tacatagtct tttacactga tacttaattt atcagctact
      421 tttttcgatg gaagttcaat tgagaaagta tggatttctg aatctacgtg ttcttcacca
      481 accacgctaa acaaaccttc gtttggacca agaactcgtg agttatcaat tggtgttctg
      541 acgacttcag tcccataccc acttctactt cgcaaaactc cttcggaaac aagtaagtct
      601 acgcctttct ttactgttaa cttgcttgta tgataaataa ctgccaattc atctcctgta
      661 ggaagtttct caccaatttt atactctcca tttacaattt ttttctttat atctaagtaa
      721 atttccatat acttcgcaat ttttttcaat ggtgaccctc caatcaaaaa catatcttca
      781 tctatacttt aacataaata aaccgataaa atccacatca aaattataca cacaataaaa
      841 aagatatata tatttaaaca aaactattta ctttaagttg taagcggtgt tatcattaag
      901 tggaaacagg aggtaaaaaa attatgacaa ctatgaaaaa gctaactttt ccagatgatt
      961 tttggtgggg ttccgcttgg tcagctgagc aagcagaagg aaaaggagaa acaggaaaag
     1021 cagagactat ctgggatcgc tggtttaaag aacaacctta ccggttttat caacgaatcg
     1081 attcctctat tacaaccgat cacatccatc aatataaaga agatatagaa ctaatgaaaa
     1141 aaactgggca taactccttt cgcgtttcta tttcttgggc acgaatgttt ccaaatggcg
     1201 gtaccggaaa agtaaatccc aaagccattt ctttctatcg aaaattattt tccgaaatga
     1261 ataataacgg aatcaaagta tttgccaact tatatcattt tgacatgcct gctactcttc
     1321 aagataaagg aggatgggaa tcacgtgaag tagttgatgc ttatgttcat tacgctgaaa
     1381 cttgttttaa agaatttggt gacttagttt gtcattggtt taccttcaat gaaccacttg
     1441 ggcctatttt aggtacttat cttgaagatt ttcattatcc aaacctcatt gactttaaac
     1501 gcggagcgca agcagcattc ttcactatac ttgcacacgc aaaagcaata caagtattta
     1561 aagaactagc tcttcctagt aaaataggcg ttattctaaa tttgagtcct acttttcctc
     1621 gtagtcaaaa tcctgctgat gtgaaagcag cagaaatcgc tgatttattt tatacgcgga
     1681 gctttttaga tcctatggtt aaagggaatt tttctaaaaa gcttatcctg ttattacaag
     1741 aatataatca gcttcctgaa gattatacga aagaagatct tgtatgtatc tacgaaaata
     1801 ccgctcaaat tcttggacta aactattacg agccacgtcg cgtcaaatct cgttcttcta
     1861 tggtggatac atctagtcca tttttaccag agtggttttt cgaaccacat atcatgcctg
     1921 gacgacgaat gaacgaatac cggggatggg aaatttacga aaaaggaatt tatgatttgt
     1981 gcatggatat caagaaccat tatggaaaca tcgaagcatt tatctcggag aacggaatgg
     2041 gcgttgcgga tgaagaacgt ttcatggatg atacagggca aataattgat gattatcgga
     2101 ttgagtatat caaagatcat cttgcttact tatggaaggc aataaatgaa ggatgtaaca
     2161 ttaaggggta tcatctttgg acatttattg attgctggtc atggatcaac gcctataaaa
     2221 accgatatgg gctggtttca ctcgacctta ctactcaaaa aagaacaatt aaaaaaagtg
     2281 gcgaatttta caagcaatta agtgatacaa atggatttag atacgatgta gacttgttag
     2341 ttgaataata ttctaaaatt ccacttgaag tgaacacatt agaaaacaag aataatgaga
     2401 aataccaata acaaatactt tttcttcata cttcatttat acttgtcagt ataagagact
     2461 gtgacaaatg tagtgatcct cagaagttag gtctggaaat ctaacttgag aagatttttt
     2521 taaactacgt ccagtctttt ttatttgttt aaaattgaag ttgacaagtc ttcattaaaa
     2581 atttagaata ttcacaagtt tgacgacaat tatgaaaatt tatgtcgtgc taaaggagtt
     2641 ttgttattgg atgaaaagtt agatataaaa actgcgatca aagacacgtt acctaccgtt
     2701 tttggttata tcggtattgg acttgcattt ggtatcgttg ggaaagctgc cgggtttcat
     2761 ccgctggtcg tcacgttgat gtccctgctg atctatgctg gttctgccca atttatcacg
     2821 gtcagcatgc ttgctagcca tagtccactg ctctccatcg ttctctcgac ctttctagtc
     2881 aattcccgaa tgattctgat gagtatgacg attgctcctt atttcaagaa aaatcggctg
     2941 cttcaaaatc tgctgattgg cacattgctg acagatgaaa gtttcgcact gggaatgaat
     3001 aaattgaact atactggaca aaagttaaac tttcgttgga tgaatacagc taattggatt
     3061 tcttatttga cgtgggtggg ttcttctctt gttggcgcac ttttagggaa ttttattact
     3121 gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg gttactttat
     3181 cttcaagtca tttctgatag aaacacctct aaacgattac agttgatcct gattggtctc
     3241 actttgattc tggtatatgt cggactgatt tttatcccaa gtaacttagt gattttagtt
     3301 gtaacactga ttgggtgcgg attgggggtg tggatcaaac atgccttctt ttgagtttac
     3361 attgctgact attttaggtt gtactttagc aacttggctt tctaggatcc taccatttgt
     3421 cttactgaaa aaattcgatt tgccgcaacc gttgcttgag tatttaagtt ttgtcccgat
     3481 cgtcattatg tctgctttat ggttcagcag tctatttacc caaaatatcg gacatctgcc
     3541 gcagatcaat ctcgaaaatg ctttggcttc agttcctaca cttttagctg ctattctttc
     3601 aaaaagctta ttggtgatcg tcttggtagg aattctttcc ctttcgttga ttcgcttatt
     3661 ataaataaaa aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa
     3721 gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc actaacaacg
     3781 acttttccta atatatgtcc tgtttctacg tctttggttg cttgtttcaa gttcttcgca
     3841 ttcatcccat ctgaatagac cttgctcaat gtactgcgga taatcccttc atttgctaaa
     3901 gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt catagttata gtctgtttta
     3961 gcgaacatat actcccagtc gaagctgata gattggtttt tccaaacacc tatatctaac
     4021 ggctctttga tccctacgat cgttcccaca tgtccaaatg gtcggacaag gtgtttgatt
     4081 tgatcaaagt agcgggtaat atcgaataat acagcgacat actctacttg atcgattcct
     4141 aattcacgaa aacttttact taagtcgtga tgataatcga ttggatagtc tacacctgtt
     4201 tttttcagcc attcaaaatt cttcggactt gcagtcgcgt agacttctag gccagcccaa
     4261 tgcgctagtt gattcaaaat cgaaccgacg cccccagctc cgttgatcac taagattttt
     4321 ttcccgcggt tggcattttc ttctggagtc agaccaaatt tttcaaataa caattcataa
     4381 gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat ctgaaagatt ttttggtgca
     4441 agagcgacaa tacgttcgtc aaccagttgg tattcttgat tgcttcctgc tcttgtcgtt
     4501 gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt gcacttcatc acctacagca
     4561 acaactttcc cgactccatc aaaccctaag atacgtaaag catttcttat cccgttatct
     4621 tggcgaagtt tcgtatcgac aggattaaca gatactgcgt tgacttttac cagaagatcc
     4681 cgtgaactgg gtacaggtat ctgttctttt tcatcaataa agctttttgg atcatcgatc
     4741 gacaaccctt cataaaaacc gactaccttc attaattcct tttctgacat acgaattcct
     4801 ccttagcttc atcaagttgg agcatccata cagttttcat ttcaatatat atagtatgct
     4861 atttgttcca cttttattca tcataattat tctcttactt aaagtaaact atttgcactt
     4921 ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca acctctttta aacggtgttc
     4981 aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa tatgaggagc tatttttgtt
     5041 tggctgttct tattacttga tgcaggacag ctttttcacg acctctaatt cacggtgttc
     5101 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtgtctaaga
     5161 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
     5221 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
     5281 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
     5341 tttctgtatg ttccctcggt tctttttcaa atgcataagg attcttgaaa atcccccggc
     5401 caatcatgat gccatctacg ccatattttt cggccaattc ttctcctgtc tggcgatctg
     5461 ggatatctcc gttgatcgtg atcagtgttt gcggagcgat ttggtcacgt aaagcaatga
     5521 ttttagggat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
     5581 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
     5641 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagccg
     5701 cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa gccactgcct tttccacgtt
     5761 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
     5821 ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
     5881 tcggctgttc atcttctgta aaggtcaatc ttcctcgtac actgtctttt ccatctggat
     5941 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
     6001 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
     6061 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
     6121 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
     6181 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaagactgt gacaaaaatc
     6241 ttatcacaat tccttttccg gaataaatgg tgttcaaaag cttgcatctg cggtaataag
     6301 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
     6361 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
     6421 agctcaacaa acgacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
     6481 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
     6541 aataccaagt aacggcgtac aaaacaacga tgaatagcac aagtacgatc atcgatgttg
     6601 ctactggcac atcgattgtt ccaaggaatc cgtaacggaa accggagatc atataaacga
     6661 tcggattgac tttcgaaaca gcttgccaaa atggcggcaa catagagatg gcatagaaaa
     6721 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcgtcat
     6781 aagactgagc aaaaatcccg ttgagcagtc ctgctaacga aaacaagatt gccgtcatca
     6841 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
     6901 tgatcgttac gagagagccg acgagaatac tgcgacccac tccgccaatc acaaagcccc
     6961 aaatgatcac atgcgtagga acaggcgcta ccaagatttc ttcgatgttt ttctggaatt
     7021 tttgagagaa gaaggacgaa gaaacgttgg catacgaact agtgatagca gacatcataa
     7081 tcaaaccagg cacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgaccac
     7141 cgatcatttt tccgaaaata acaaagtaca aggatgtcgt gatcacaggc ggaaccagtg
     7201 tttggaccca gatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
     7261 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaaa
     7321 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
     7381 gtcagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
     7441 tgttcatctt caaatgtaca agcgtatcct tcaatcacag gtttggtctc atatggcgct
     7501 aaatcaaaga taaatgtttc aaattgcagt ttggatagaa gctctttcat actagtattc
     7561 tcgatcagtt cacctgattg gatgatcccg atgttgcgac aaagcatttc agcttcttct
     7621 aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg gagaaactcc
     7681 cacatctcgc gtctcaattc aatatcgact cctgctgttg gttcatccaa gatcagcaaa
     7741 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
     7801 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
     7861 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
     7921 tggaccgttt caaagggatt gaaattgaac tcttgcggga ctaatccaat ctgttgcttt
     7981 gctcggacta gatccgtatc cagatcataa ccgaagacat tcacttttcc agatgtttta
     8041 ttgactaaag aagtaatgat tccgatcgtt gtagacttcc cagctccatt tggtcctaat
     8101 agcgcataga aatctccttc tttcacggaa agatcgattc ctcgcaatgc ttcgacccca
     8161 cttccataaa cctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
     8221 ccttatagac atgattcttt attgtatcat ggaatacagc agatgaaaaa acaacttggt
     8281 tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc taatgaaaga catcaatcgg
     8341 acgaagtcag acaacctttt cagaaaaaac aaatcgaaga taaaggatta ctattcatat
     8401 gtgcaacaaa aggagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
     8461 gtataaacgg cggaagcaaa agccaagatt tttgatccaa taaatacttt cgatcacttc
     8521 gcctatccca atacatgcca ctgccgcttt tcatcaaata acgcggcttc cgtttttgcg
     8581 gtttctacta aagtctctgg gtaatccaaa actttcagca aggcaatcgc atttcgggtc
     8641 cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt
     8701 tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt
     8761 gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa
     8821 gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat ctacgaagca gtagcagcgt
     8881 tctttggaag ccacttggtc caacaaacgt ttgatcgatt tgatttctga aacaaaataa
     8941 ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc
     9001 agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct
     9061 atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc
     9121 tgccagttga ccggattcac taccgcttct tttaacaatg gatgatagat atctttcgct
     9181 gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg gcatataagt acggtaattc
     9241 aatattgcag cggctacttc taattctccc agcaattccc acagcttgat ggcttcagtt
     9301 gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg
     9361 atgttgacat attcaaaaat caactctgct tcactcccac tcttcatacg aaatgaaaaa
     9421 cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttc ttgaagtggt
     9481 gtcggcagtt ttgctagctg attggcggct gaaatcgttg aaaccaaata actcatgcta
     9541 ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaac
     9601 ccaccgattg ccaaatagat tcctagtact tgtcctaaaa gaagcaaacc caaacccacc
     9661 agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca
     9721 tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat
     9781 tggatttttt ctctcagttg aggattttcc tgataaaaag caatcagttt ttctaactgc
     9841 tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca acgcttctga acctacacta
     9901 gaataggtac tgttgattcc ttcgaatact tcgaacatat ccaggtcata ccaagtaaga
     9961 tcatcgattt cgctatccca tgaacggaac ttcttctccg tgagccatgc ttccttcaag
    10021 ctttcttctt tatcaaatct cggttgataa ggaatttttt cccatttgct acgaactgtt
    10081 tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta ataaaataac agctgcaata
    10141 ataattaaaa cgataaattc tctttgaccc atatccctct ccgttccttt atttgttagc
    10201 tttagtatag cactgttgcg ctatttgaaa agagggtaaa aaaacacctt tcaaaataaa
    10261 aggtgttttt ttcttgtata ttacatattt atctctcatt tatcaaaatt gacaaataat
    10321 catagggagg aattgttgtt aattcttgtt tattctccag tgcattgaca actgtcccaa
    10381 gggaaaggat agcctcaggt actatttttt cttgttctgt tggattagct aagcgaagaa
    10441 caaatccttg ttcatgaaaa taagaaggat aaatcgatga tacaattaag tcttcaggta
    10501 attcaatcaa tgaaaattta cgaggaacat caagttcatt ttgtaatggc caaatcttgt
    10561 tatctaacct gtgaataaat acattcagtt tctgcttttg ataagaaata gattgttcta
    10621 accgtttttc tgcctgtttt gccactttga actcattaaa tcttccttct tctacattaa
    10681 atccaaaagt gactttatac tcacctatct cttgagctag cggtgtttcc atcatgacat
    10741 gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg ccaagcaaga ttaggttttc
    10801 caagttcccc tgttgttgaa aataaggtta tgattaattg attttctata cgttcatact
    10861 cttttaaacc atctgcataa aacgtaaaat aatgatcatt ttctgccaca gagacactct
    10921 tatcgaatat ttctaaatta acaggtttct ctaccatagt ttctgaccaa ttcggaggaa
    10981 tgacatcagg ggtattttca ataaatccgt tttgaatttg tgcaattgat ttttcagatt
    11041 tatctaaaac attaattgct aagcgcatac gatgcgagta aacagtatta tgaattttga
    11101 gtgtgccttc aacgatttga ctttctccca gactgaacga tatttcataa gatatttctt
    11161 ccatttcggg tttttcagat aaacgatctt ttaaatcttt aggaagttga gcttttccgt
    11221 aaacaactaa tgtttcttgt aaagaatctt tatatacttt agctgtttct aattttagag
    11281 atagttctgt atctccttct aatggtgaat aatcatacgt atcgccatca tttgctgaat
    11341 cgatcaaatg aacgaaatca agatattgtc tatctccaac tatcaaattc aattgcccat
    11401 ctttaaaaga aagtttatat ttattgttac ttatctcagt tttgtctatc tcgattgctg
    11461 tttctaattt ttcattattt ttctcaaatt caacaacagt gtatcctagt gctggcacat
    11521 ttacatttat ttttatgtca agttcataat aagctggttc atcaaaatat tcgaaacctg
    11581 tagcaatcat ccgataagca tttttccgtt ctggaaaata aacttctttt tcaatgatat
    11641 tatagggact acctttgaag tgtattttct ttgaatcagc tactacatgc acgattttcg
    11701 atccactata attctcagtc gttgtgttga aaacaaggac ttcattcgat tttagcttta
    11761 atgcatcagc catccgtttt acaataaggt tttcgattcc gtcagctatc tcattagctt
    11821 ctttgattcg atggaaaata tcttccgcaa cattatctga aacacagcca ccaatactat
    11881 catgagcctg gttttgcaaa atctttttcc ataaccgacg aagtaggcct ttactgatat
    11941 ggataccatt tctttctgca atcagcatga gcggctcaac tcgttttaga attttttgtt
    12001 ccaatttaaa attttctatt ttcatttttg ttcttactga gccaatagaa cgatgaacac
    12061 gagagtaaac cggctgacga agttcacctt tatattcagg taattctttt ttgtttctga
    12121 taacttctac ataagaagga taatctgaaa taatattgtg gaatttactg tatttattca
    12181 acttctctac gacttgatca aaatttaaaa tcattgactt ttgatctaca cccgaaggta
    12241 ctaaaatatc tttgtttttt ccttgcatat gaagcatttc aactgcttca tccaactttt
    12301 cttttgcaaa ttcaggaata tctttaattg cgtctaaggt catcattcca gtcatataac
    12361 caaatggaaa attcatggca taaacaaatt tatctcccaa tcctttccag ataaaataag
    12421 gagatggaac catcgtttca aagtttaatc ctcgccaaca catgaaatta tcgatttcta
    12481 cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa cgtgtcaggc aaatacccta
    12541 ccatcattgg atctccatat ttaaggatcg tatcactgat tccaatcatt aagtttctta
    12601 aaattgattc agcatccact aataaggcat ctgtttgtgc ataccacggt cccacgaata
    12661 gttgtcctgt tttcatcagt cgttggataa ccttaagctt ttgcggattg atctccaaat
    12721 actcatcgac gattgaagtt tgtccatcta aacaaaagtt tactttaggg ttcctttcca
    12781 attcatctag aacttcagta aagacctgat cagacaatac gatggcatct tcatttgtaa
    12841 aataccattc tctgtcccag tgcgtgtgtt gaattatatg tgcatttacc atatcagatt
    12901 ctcctacttt ccttcttcta ctgaatttac ttgttgttct tttttatact tgatggattt
    12961 tagtgcattg accattaacg ctgtaaccac tcccccaata acaattgata ctaaatataa
    13021 aggtatattc gtaacaaaag gaattgccat aaaggttgaa agcattgttt cttgagtaac
    13081 accaaaaaat ccatttagac cacatgctac tgcagtgccc accacgatgc ttggtattac
    13141 tcgtaaagga tctttcacgg caaacgggat cgtcccttca gtaatcccca caaggcccat
    13201 aatcatagct gtctttgcat tatctcgttc cgattcatca taaagttttt tgtttaaaat
    13261 tgttgctaaa aacattccaa atggcggaat agcaattcct aataacatgg ctgtactagg
    13321 tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca tatgcgattt tattgatagg
    13381 tcctcccata tcaaacgcca tcattcctcc taaaatagcc gacatcaaag cagcatttgc
    13441 tcctgacatg ccttctagcc aattagtcag tcctgttgtc attgcttgaa taggcgtagc
    13501 tagtacatac cataatatga ttccaacaac taatgtagta acaaaaggaa tcagcataat
    13561 tgattttaga ctaataactt gcatcggtac ttttactttt ttcaatgcat tagctaaata
    13621 gccacaagct aatccagcaa ccattcctcc aataaatccc ataccaagat tttgacacat
    13681 catcccagtg ataaaagctg gtgctagacc gggacggtca gcaattgaat atgcgatata
    13741 ggctgaaatc atcggtacga taaactgtaa tcccgtctga ccaatcattc gcaggactcc
    13801 ccagaatgta tccgtctggt cccaaacacc tgtgccacca aaaatgactg caatagaagt
    13861 tagtaaccct gctgctacga caagcgggat catataggaa accccacttt ggaaatgatt
    13921 tagaatttct cttccttgtt tcttgaattc cattagtaac tcccccacga tatatttttt
    13981 atttgttaat taatgcaatt gcttcatcaa tcgtctcttc accatctttt actacctctg
    14041 aggtccctac tgttaaaatc ggtttaccgt caaagcgacc tcgttctgaa acatttgtat
    14101 ctactgcaaa tatgacaaca tctgcctcgg aaacatccga aattgaaagt ttgttttcta
    14161 tccccatagc cccctgtgtt tctactttaa tatctaaccc tttcttcttc gcaaactttt
    14221 ctaaattagc ttttgccata taagtatggg caatccctgc gatacatgct gttactgcta
    14281 ctatttttgt catatgaaat tcctcctttt aaaatgatag agcttttacc aattcttttt
    14341 tatttctcga atcttttatt cttttaacaa actcgtcatc catcaatctt ccagataatt
    14401 ctgctaaaat acgtaaatga ttagtacctt tatcagtgtc ttttattgct aacaaaaaga
    14461 cgagatcaac tggttgatca tctaatgcgc cccattcaac agaccgcttt aacttggcta
    14521 cagccactgt tgattcaatt actgctaggc ttttgccatg aggaatggcg atatttttcc
    14581 caattcctgt ggtcatatgg ttttctctgt caaatacgtc tttttgaaat tgtttgtggt
    14641 cagacacaaa cccattagtc tctaacaatt tcgataattt ttcgactaca ctttttttat
    14701 cagatgcatt taaatctatc actatattat ttatattgat tttttcactg atcatttata
    14761 agccttcttt caatatataa actaattttt caaacgacga actcgttttt aaatttgcta
    14821 ataactcatg attcgaagcg atttcattga atgtttcata aattttattc aattcaggtt
    14881 ttactttttc attaagtgca aaaaagaaaa cagaatgaac aagtgtccca tcccaatcaa
    14941 ttccttctgg attaacgtag acacagatta ctggcttttt tataaaatct acttcgatat
    15001 gcggcatagc aaaatcattc atcgctgttg aagatagttt ttctcttttg attgctccct
    15061 ctagtatccc ttctttcgcg taattttctt ttaccaattt tttggtaatt atatttaaaa
    15121 cgttcacttg attatccttt atttggtggg taaaaaagca aaatcgttta tctaataaat
    15181 ttagaaaagc atcttctgtt tgttcattta actgtagata ctgttttact tttctgacat
    15241 ctgcccctaa catcaaagga ctaacaacaa taacaggaaa atttgtattt tcaatcggaa
    15301 tcgtgcttat aacaagttca tcagtgactt ttctatcttg tagttcattg atgcttagta
    15361 catcttctat ctcaaccata tctgcaaaac cgttatttat tctttgctct aataattttg
    15421 atgttccata tccgctacta caaactaata tcactttcgt tttgtcaggt ttgtaacgat
    15481 ccaaaagtga ttgaatatgg agtgttacgt aagcgatttc atcttcatta aaatgtatac
    15541 agaatctctc ttcgatttcc tctaataaat ctacacttat cataaatgac cttttaaaag
    15601 aatatttgat tttgtccgta taagggttat agatatttac tcccaatttg agtcttttaa
    15661 ctgctgc
//
LOCUS       DAZKMB010000063        15451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000063 DAZKMB010000000
VERSION     DAZKMB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.063"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1016..1357)
                     /locus_tag="ACG8V3_001757"
     CDS             complement(1016..1357)
                     /locus_tag="ACG8V3_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0300293.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1357..1587)
                     /locus_tag="ACG8V3_001758"
     CDS             complement(1357..1587)
                     /locus_tag="ACG8V3_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0300294.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1874..2212
                     /locus_tag="ACG8V3_001759"
     CDS             1874..2212
                     /locus_tag="ACG8V3_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300295.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2417..2979)
                     /locus_tag="ACG8V3_001760"
                     /pseudo
     CDS             complement(2417..2979)
                     /locus_tag="ACG8V3_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3711..4292)
                     /locus_tag="ACG8V3_001761"
     CDS             complement(3711..4292)
                     /locus_tag="ACG8V3_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0300296.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4475..5101)
                     /locus_tag="ACG8V3_001762"
     CDS             complement(4475..5101)
                     /locus_tag="ACG8V3_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0300297.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5123..6454)
                     /locus_tag="ACG8V3_001763"
     CDS             complement(5123..6454)
                     /locus_tag="ACG8V3_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0300298.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6609..7010)
                     /locus_tag="ACG8V3_001764"
     CDS             complement(6609..7010)
                     /locus_tag="ACG8V3_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0300299.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7096..7302)
                     /locus_tag="ACG8V3_001765"
     CDS             complement(7096..7302)
                     /locus_tag="ACG8V3_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300300.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7397..8899)
                     /locus_tag="ACG8V3_001766"
     CDS             complement(7397..8899)
                     /locus_tag="ACG8V3_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0300301.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACG8V3_001767"
     CDS             complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACG8V3_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0300302.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9470..10087)
                     /locus_tag="ACG8V3_001768"
     CDS             complement(9470..10087)
                     /locus_tag="ACG8V3_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0300303.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10308..11681
                     /locus_tag="ACG8V3_001769"
     CDS             10308..11681
                     /locus_tag="ACG8V3_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0300304.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11756..12868
                     /locus_tag="ACG8V3_001770"
     CDS             11756..12868
                     /locus_tag="ACG8V3_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0300305.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12940..14577)
                     /locus_tag="ACG8V3_001771"
     CDS             complement(12940..14577)
                     /locus_tag="ACG8V3_001771"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0300306.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14745..15353)
                     /locus_tag="ACG8V3_001772"
     CDS             complement(14745..15353)
                     /locus_tag="ACG8V3_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0300307.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4281 a         3168 c         2694 g         5308 t
ORIGIN      
        1 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
       61 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
      121 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
      181 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
      241 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
      301 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
      361 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
      421 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
      481 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
      541 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
      601 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
      661 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
      721 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
      781 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
      841 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
      901 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
      961 ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa
     1021 acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt
     1081 ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt
     1141 ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa
     1201 ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc
     1261 ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat
     1321 cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg
     1381 agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg
     1441 atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc
     1501 ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc
     1561 ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag tatactatat
     1621 aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag
     1681 tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa
     1741 aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta
     1801 accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac
     1861 aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa
     1921 atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa
     1981 agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc
     2041 tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac
     2101 tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc
     2161 caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac
     2221 atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac
     2281 attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc
     2341 tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt
     2401 tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga
     2461 tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa
     2521 gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg
     2581 attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt
     2641 ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg
     2701 attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt
     2761 aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag
     2821 gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc
     2881 tgctaattct gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag
     2941 gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt
     3001 tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga
     3061 actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc
     3121 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg
     3181 tgactcggag tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt
     3241 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct
     3301 taatactcga aactaagcga ctttttcaca acctcttcct taaacggtgt tcaaaagctt
     3361 gcatctgcgg taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg
     3421 ttcttattac ttgatgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc
     3481 agctaacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
     3541 tttctctaat actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga
     3601 agtagcttct tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
     3661 attccttctt atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc
     3721 ttttgcgaag ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga
     3781 tcctgtgtga ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct
     3841 tgacggtctg cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc
     3901 gtatacttga cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact
     3961 gtactcaact caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca
     4021 tgatcagcga aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca
     4081 tttaagatgt cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct
     4141 agacaaactt ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa
     4201 gctgctagaa tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg
     4261 gtcgcttcca agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag
     4321 tcctatatta tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc
     4381 caaattatct gacacagccc tcactataaa actgttctca tatagctaga tgttcattga
     4441 ttcaagaact gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct
     4501 ctgttgtcat cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt
     4561 cttggccagg agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctacca
     4621 attcacgcat atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt
     4681 cgattccatt ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat
     4741 gaggagagct gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg
     4801 ctcctgaacc ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa
     4861 gcatcatatg ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat
     4921 gaggagaaat aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat
     4981 tttcgatcgt attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg
     5041 gtttcaagaa ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca
     5101 tggtggattc tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg
     5161 gatgagaata gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg
     5221 aaatcaagtt gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt
     5281 cagtttcagg gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt
     5341 tagggacacc gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt
     5401 gtttttctgt taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag
     5461 ctttccggtt attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat
     5521 aatcatctag agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa
     5581 tgttgtctgc atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt
     5641 taacatctgt attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca
     5701 aaacttttga cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg
     5761 ttgattcttg gtctgctact tgatctacag atacacctag atggaattcg attcccgcat
     5821 ttgtcatgtc ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag
     5881 aatgggcatc aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca
     5941 agccaatata tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg
     6001 cttgcgtaga atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg
     6061 gttcagaacc agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat
     6121 tcgtcacttc gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag
     6181 ttgcctcatc gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt
     6241 tccgagcaac agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag
     6301 aaggaataca gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga
     6361 ctaaaaccga ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa
     6421 aaccgatcac aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac
     6481 cgattggtac aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt
     6541 tgtcaaaaaa aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct
     6601 ttattcttct aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa
     6661 gggcagaatt catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg
     6721 atatgcactt ctaatactac accgatctga tagccgccat catctaaatt ataaagagag
     6781 acacgtgctt ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc
     6841 gccccattga aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct
     6901 gcatggattc ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct
     6961 ccattgcgcc cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc
     7021 ctttcgattg accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt
     7081 ctacaaaaat tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa
     7141 caaaaaaata ctactcgtga acaacagcgt catatttgtt ccaaaataaa tagcaatctt
     7201 ttctacataa tcttttatat gattgatttt aagatacata tataccagaa agttgacccc
     7261 ataaaccaat actgttaaaa aaataaccat attcgttacc attttttcct ccttttgctg
     7321 gtctccctac cagcactcga tttaaccata ccactttttt tgccgtccaa gcaactagcc
     7381 tttcaaaaag actggtttat tctgctgtta atgattcaag atcagaaaaa tgtccgaata
     7441 attttgctaa ttttactaac gccaacttgt tctttccgaa ttcatcattt tctatctgta
     7501 atactagtaa cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct
     7561 cttctagatc atatcgaata ccttcttcat tttccgggtc gaagttcatc cctttttctt
     7621 cttccaacag tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc
     7681 tgaacaattt taatcggatt tcttgtactt ctacgggctg tttcaactct ttgatttgtt
     7741 catctaatgt gatacctttt ctttttaatt ctggcaaaag catcaggatc tttgcgatga
     7801 catatgcacc atcatccaga aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga
     7861 tggctagctg tgtatctttc ccaagacggt taagttctaa tgctttattg attacatttc
     7921 gatagccgga aatataacga acttggatgc ctcctttttc ttcaatgaat ctttgtagat
     7981 gagaagaagt aggggaattc gtcacgattc ttgttcctgg atgctctttt aatacgattg
     8041 tacttaatac tgcgatcaaa ttatttcgat tgatcactgt tccagaagca ctgacaactg
     8101 cagaacgatc cacatctgta tcgaaaatga tcccaagatc tgcttcattt gctaaaacag
     8161 cctgcttgat actttccatc gcttctttat tatcaggatt tgggatatga ttaggaaatc
     8221 ggccatctgg ttccaaaaat tgggaacctg ttgaatccgc acctaagcta ttcaatactt
     8281 tttcagcaaa gaatccacca gcaccatttc cggcatcaac aatgattttc caaccattta
     8341 gtggtttgct ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat
     8401 aaggtgtcaa aaggtctgct ttttctacgc ttccagtttg ttcgcttttt attttttgct
     8461 gctgtgatag gataaaagca atatcttctt tttctgctcc tccttctgag gtaaagaatt
     8521 ttattccatt ataataatag ggaagatggc tagcagtcag cataaccgca gcatcgcacg
     8581 agaactgttc aaattgagtg gacataaaca tagcaggcgt agtagctaat tcgaaatcga
     8641 tgacatgtat cccttgttct ttcagacttt ggatcaacgc ctctttcagt gctggtccgc
     8701 ttatcctact gtcatgacca atcccaatcg tccatttttt atttggatcg gttgatttct
     8761 tctttttctt tatccagttt gctagtcccc agccgatctt ttttaccatg tctgtcgtca
     8821 agtttacttc atatccttca actggtaatg cgattcctcg aatatccgat ccattttgca
     8881 gcgctttcat atctttcata taaaagtcct cctcatttat tccctcattt tttccagctt
     8941 ttctgcaaat cgctgtgctt cttcttctga gcggcaaata ataatgaatg tatctacacc
     9001 ggccatcgtt ccgacaactt cagggtaagc cacttcatct agccaattcg ttaccacatc
     9061 ggcaccattt cgttcagtca atacgataac catgaactga acagactgga tacgcaaaac
     9121 ctcgcgtcta attgctgtac gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa
     9181 taatgtatag cgtactattt tgttttcatt ttgacttttt actaacttca tttctcgaat
     9241 gtctcgagaa atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag
     9301 ctcttcctgt gtcccaatag gttgttctgt aatgatctgg cggatcagtg cttgtcgctg
     9361 tttctttttc atattagaca cctctcaatg ttgattatag caaaagataa gtaatatttc
     9421 ttcatatttt ggtatttttt acttttattt tttgttttca ttcaggttgc taaccttttt
     9481 ctattttaac acgcaaaatc gtcttcattt tttcaggtgc tgattttctt ggatcgctga
     9541 tgtaaatctc tttgtgttct ttagaagctc tttcatagcc ttcttttgcg atgaatgctt
     9601 ctaatttctc aaacgttgct ggttcttcgg catagggccc gacgtgtaag atttgggcac
     9661 aaagaccttc ttccatcttt ttgaatacta cttgttcttt caagtcttcc ggaagtttgc
     9721 tagaacgctg aatggcttct tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca
     9781 tcaattgata agaaaaatgt tcttttttca tcactggttc atgcaaatac ttttcttgca
     9841 gtccccattg tccttccagt ggatagacag tatacatctg ataatcagga atcagccaat
     9901 tttttttagg cgacatgcgt attgcatagc tgactgcata tagacaggct actcttcgtt
     9961 taaagtcttc ctgattcggg tcgccgatcc ctctgatgct aaagaatgat tttttaggaa
    10021 cctctagtat ttgcgggctt tttttcatac tatagctctt gtctttacgc cattcaattt
    10081 tttccatttt tcttcctccg cgaaattcca tttagttatt tattatcata cctctttttg
    10141 agtgacttgg ctaatcgtgt tgacactatt tttcagtttg tgctagaata agtgaagtaa
    10201 ataaatcgta cagataacac gtttcggcgt gtcgagagag aggcggtttc tatttttata
    10261 atagaaatca ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa
    10321 aacagctgct tttagtacct gcagtaattg caacagggat gatgtctttt gcgggcgtat
    10381 tgatcgagac ggccatgaat gtaactttcc caactcttat cagacagttt gatttaacga
    10441 ctgcacaagt ccaatgggta acaacgattt atcttttgat gatttcgatc attgttccgc
    10501 tgtctactta tttgaatcgc aattttagtc tcaaaaaact ctttttagct tcaaatctgc
    10561 ttttcttggc aggtataatg atcgatttct tctctcctac cttcatggtc ctattgtttg
    10621 gtcgattgct ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc catattatct
    10681 tgacatttgc ccccctcaat cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg
    10741 ccattgctcc agcaattggc cccacttatg gaggcattat gacttcacat tttacatgga
    10801 atcatatctt cctgtttctt gtgcccgttt tgtttctttc tttgattatc ggctggtttg
    10861 ctattcccga gatggcagta caaaaaagtg gttatttgga ttggataagc gttttaggaa
    10921 tttgtctttt gtttagtgga agtctaacat ttttgagtac gttgggaaca gtttccagct
    10981 ggattgcttt aatcatcggc ggtattggct ggttcctttt ttactatcga agtaaaaaag
    11041 tggagcatcc actgatccga ctgtctattc tttcaaataa aacattccgc ttgtttttga
    11101 ttggcttctt agtttatcag tttttattac ttggtgtatc ttttgtcttg ccgaatttcc
    11161 ttcagatcgt tcaaggaagt agtccttttg tcgctggatt agcgatgctt cccggcgcag
    11221 cggttggtgc tttgctttct cctttttccg ggaaaatgct ggatcaatat ggtccgaaga
    11281 agccaatttt tgctgggctg attctttctt tgattggttg gctagcatta ttcctactca
    11341 taggacacgc ttcagtgggt gtattgattg cttgtcatgt gttttatatg atcggtatcg
    11401 gattatctta cagcaatatg atgaccaccg gaatgaatgc actgaatgaa gaactacaag
    11461 gagacgggaa cgcaatgttc aataccttgc agcagttttc tggcgctgtg gctacttcat
    11521 tagtagcggt cattatcaat tacgtccagc atcatacttc ccataactat gaagtgtcta
    11581 ctacattagg atcaaaagca gcattaggcg tcttgttgct gttgcttctc gtaagctttg
    11641 ctcgattcgc ctattatctg ttctttgcta aaaaagcgta gattccatat acggcaggtg
    11701 ttgcatctgc cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct tttttatgga
    11761 aaaaattgcc attatcggtg gcgggatcat tggtatgacc ctagccagtt acttagatac
    11821 atcaaaattc aatgttgctt tatttgatga agggacagga caggcaacta aagctagcgc
    11881 tgggattatc tccccttggt tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa
    11941 agacggcgct gcttttttcc ctaaacttat aaaagatttc caacttgatc aaacgattta
    12001 cgcacaatca gggacacttt tgcttcgacc tgaaaaagat ttagctgaat tggctgttct
    12061 tgccgagaaa cgaaaagaag aagcgccaga aattggaaac atcgaatggc tttcacctga
    12121 acagacatct caagcacttc cgttattgaa accagcagtt tcactgcgta tcagtggtgg
    12181 tggtcgtctg gatggaaaag catatttaga acatcttggt cagcgtttag cacaaaaaca
    12241 aatggagcag atccaggcaa aagtcacttt gcataagaag agcgattctt ggctcatcaa
    12301 gggaccgttt ggagaataca cagctgatcg tgtaattttg acacctggcc cggcacttaa
    12361 gcctttatta caagaattag gctatgctgc agatatacgt ccgcaaaaag ggcagctctt
    12421 agcatttcag acatcttata agatgagtca agactggcct gttgctatgc ttgacggaga
    12481 agctgatttc attcctttca tggatggaaa gatcctttta ggtgctaccc atgaaaacga
    12541 tcaaggctgg gatttggctc ctactagcga agcttataag ggattgacta agagtacaga
    12601 acagttcttg actgatccag atcagataaa aaactggcca tacgagttcc gtgtaggtac
    12661 ccgtgcttat acgtcagatt tttccccatt tttcggcgta ctgccagatg atccaagctt
    12721 actggctgct agcggtctag gctcttctgg cttgaccaca ggtccttata tcggctatct
    12781 attagccaac ttcttaaata ctggtcattg gcctacggaa gattatcaaa aggacatctc
    12841 cacctatatt acgaaaaatc catcatagaa aaacagcttt ttcaagtaag gatcccgagc
    12901 gatattgcgc atggattact tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa
    12961 attctccaaa ctttcttttg cttcatctgg caagagatct tttcctatct gagcttcaat
    13021 gaatcgtaaa cgaatatcaa gtcgttgttt caccagattt ttataggacg tatcctcaaa
    13081 agaaacagga aaatctggta taggtaaata gtggatgtct tcaaatggac caaacgactt
    13141 gaattctgcc gtttgttgca cgattctttc aacgattgac aatgtcgttt caggtttgat
    13201 ttttttatca tcgaaatatc cagtattcag gacatagcag gctgtttgct gtttcaaaaa
    13261 caactctcga aaagcttgat agtcttccct aagaggatag acacggaaag gattagcaaa
    13321 tggttcaatg actagctcat ctagctgtct ttttgtagtg atattttccg cgtctgatcg
    13381 ttttgttgct aatgttgcac caaaagacgc tgctaaaact gggtcattga tcttgataat
    13441 tggcggaagc gaatcatctt tcatgatcca aaaaacagca tcgatctttt cgctaagatg
    13501 atcgactcgg tcgggtgtcg cataacgtga tttgactgtc cgcccatttc cgtttcgtat
    13561 atctcctgtt actaggactt tttctccttt acttgtacat gtcacgccca cattctgaca
    13621 agtaaggaaa gaagaaatgg ccggatcatc agacggatag tcttgtgttt tatcaaagta
    13681 agaaggttcc aaggcagtag caaaaccatt ttttcgagaa ataacgaaag catcgtcatg
    13741 caagatcgaa acagagtgac cttccgtatg ttttgctaag gtaatcgttg attttccaga
    13801 accggacaac ccaaatacag ccatcgtata ggtctggtca ttactagcat attgtttcat
    13861 cccaccgtgg caggcaataa agccattgcg atttcccaat gcccaagcaa gcgtcaaagt
    13921 cgcttttttt aattctccaa agtaacgtaa tcctagcaca gccgctgtat tatggagagc
    13981 atcaaacaaa atgagtccat ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat
    14041 ataaagatct ccctcatcaa aggaaacaga cttttgataa gtctctaaga tcttcgaatt
    14101 cgctggctga aaattcagca aataagaata caaattgttc tcaaatcctt ctggaatcag
    14161 caaatgactt tgcacagaaa attcttcaga taggccaacg attacttttg tatggtaaaa
    14221 ctggcgcttg cgactttgat acacagcttc tcttaaaatt ttctcgtatt tgctttgttc
    14281 cttgtctaag tcctcaaaga tccgccgagc acttgctgtt cttcccacaa tgcttccatc
    14341 attgaatact agtgtcctaa catcagctgg taaatctagt tcttcagcat gtgagattgt
    14401 taaatctgtg atgatcgttc ctggactccc ttttgctagt tgataagctg tcttcctatc
    14461 aaaaacttca tgaacattat taccgtaaaa tgtcgtttct acaatcgatc gaatactgga
    14521 taacagtgga tttccacttg tgatttcgtt taattcgaga ctctcttttg tactcatggc
    14581 tcctcctcat tccctaaaca aaaaaacgtt ttctttatta tacattattc gagtttttat
    14641 ttgtactgct gcgctgtggg aagcaaacga gttatttttc acacaaaaaa accgagaact
    14701 tgaataaaca agtttctcgg tttcatcatt ttccttttat cggcttagta ccagccgttt
    14761 gctaaccaga atgttttagc agcatcccat gaaccataac gagatgttac atattgttct
    14821 gctacacgtt cttgattggc agctgaatag tcgccgttta agtatgaagc atctaattga
    14881 taacggccga tgtaacgtcc gttagtagct gtgtatgaac cgcctgattc tttttgtgcg
    14941 atccattctt ttgcagagct tgaagttggc gctgttgcag cagctggggc agcaggagct
    15001 acttcaactg tttcaacagg tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta
    15061 gatgctgtat tttctgttgt tttgttttct gttgttgctg aagaatcatt tgaagttggg
    15121 attgttaatt gttcaccaac ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca
    15181 attgcatcga tcattgagtt gtctcctgca aatttttgtg agatcttaga taatgaatca
    15241 cctgatttta ctgtataaac ttcatcagcg tgagcatttg tagttcctac aaacattgca
    15301 gtagcaccag cagcaaaagt tgtacctaaa acaattttct taaatgaatt catattaaac
    15361 tccttttttt ctccgttttt tattctttcg acgctattaa ctttatcatt tagaaatatt
    15421 tcataggtaa aagaaacatg acatttaatg a
//
LOCUS       DAZKMB010000064        15423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000064 DAZKMB010000000
VERSION     DAZKMB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.064"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..2213
                     /locus_tag="ACG8V3_001773"
     CDS             153..2213
                     /locus_tag="ACG8V3_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0300308.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2296..2865
                     /locus_tag="ACG8V3_001774"
     CDS             2296..2865
                     /locus_tag="ACG8V3_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0300309.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3040..3846)
                     /locus_tag="ACG8V3_001775"
     CDS             complement(3040..3846)
                     /locus_tag="ACG8V3_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300310.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3984..5498
                     /locus_tag="ACG8V3_001776"
     CDS             3984..5498
                     /locus_tag="ACG8V3_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0300311.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5901..6884)
                     /locus_tag="ACG8V3_001777"
     CDS             complement(5901..6884)
                     /locus_tag="ACG8V3_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0300312.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6889..7536)
                     /locus_tag="ACG8V3_001778"
     CDS             complement(6889..7536)
                     /locus_tag="ACG8V3_001778"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0300313.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7621..8253)
                     /locus_tag="ACG8V3_001779"
     CDS             complement(7621..8253)
                     /locus_tag="ACG8V3_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0300314.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8269..8820)
                     /gene="coaC"
                     /locus_tag="ACG8V3_001780"
     CDS             complement(8269..8820)
                     /gene="coaC"
                     /locus_tag="ACG8V3_001780"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0300315.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8817..9554)
                     /locus_tag="ACG8V3_001781"
     CDS             complement(8817..9554)
                     /locus_tag="ACG8V3_001781"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0300316.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9551..10540)
                     /locus_tag="ACG8V3_001782"
     CDS             complement(9551..10540)
                     /locus_tag="ACG8V3_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0300317.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10798..11340
                     /locus_tag="ACG8V3_001783"
     CDS             10798..11340
                     /locus_tag="ACG8V3_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0300318.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11357..12442
                     /locus_tag="ACG8V3_001784"
     CDS             11357..12442
                     /locus_tag="ACG8V3_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300319.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12454..13260
                     /locus_tag="ACG8V3_001785"
     CDS             12454..13260
                     /locus_tag="ACG8V3_001785"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0300320.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13261..14088
                     /locus_tag="ACG8V3_001786"
     CDS             13261..14088
                     /locus_tag="ACG8V3_001786"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0300321.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14085..15158
                     /locus_tag="ACG8V3_001787"
     CDS             14085..15158
                     /locus_tag="ACG8V3_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0300322.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4960 a         2784 c         3139 g         4540 t
ORIGIN      
        1 aagttattat tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact ctcctaatag
       61 tccatcttct tttgatttta tttatataat gaaatcatct tatgaaaatt actacttcat
      121 gtttagacaa gagtagggag gactaagaat aaatgtgttc aaaaagacgt tcaaaacaac
      181 taaataaaca cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa cactacgtta
      241 agtttatgat gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg catgtacccg
      301 ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc tgtttctgag accactgatt
      361 cccctatcac tattactcat actttcactg atgcaaatgg aaaacagcta cttcctgcat
      421 tgacttccta taataaagtc agttatcctc caacgataaa ccactactgg ttaattccta
      481 acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc gctttctatc ttttccgata
      541 tgactgatcc tacaaaaggt gatcagttaa aacagatcac tgatattttg aatcatgctt
      601 tgacagatat aaaggacggt ggaagcgcac aagtttctta tgtatatgaa aaagattatt
      661 cacagtttga aagaaagaaa atcgatgttc tggcagggac aaaagtttcg atatctgatc
      721 tggtagaaag gctgaagaac gcagatggaa cacaaggaga tatgtccaaa gtaatttcaa
      781 acaatgggaa tatcgtggat acgactaata caggaacatc tactatttcc ttgtcctatt
      841 ttgatccaat tgctttaaaa acaatgactg catctgctgt gatccatgtt cgtgctgatc
      901 aaacagctat caaaggaaaa gatcttcact taagaacagg agagagttac gcccctaaag
      961 atcttgttga atatgtaaca aaaagcgatg gaacttcagg aaataaagaa gaaatcaaaa
     1021 tgaacgggac agtagatact acaactccgg gagaatatcc cgttgtatta acttatatag
     1081 actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa gcatctgctg
     1141 cttcatcgac tgtcatcact cattttatag atagtcaaac aaataaagaa cttcaagcag
     1201 aaacacatat gagtagtgta aatcgtattc ctgatttgcg cctacctgct gaattttctg
     1261 atcgagtgcc aaatatagat ttatcgactt atgaacaaaa tgggaaagtc acttctattt
     1321 ctgattggat gcaagcaaac ggattagaca gaaaaagtca ctggtcggag atccttgaag
     1381 tacttcactc agaactgatg aataatagga agcaatcagg aggggaaatt acctttactt
     1441 atgtttatat accagacaat cgttcattag aaggtcatga tcttattgtt catccaaacg
     1501 aaaaaataaa caaagaagag ttgatccaat cagcaacaaa ttccttaggt aaacccgtac
     1561 cgttaaagga tgtatcggtt acaatcaatg aagtaccatt ggaggatggt tatttgacca
     1621 aacaaacagg tgattttacg attatgtata gttatcatga cccgtattca ctccaagaac
     1681 tccatgctca gtcactatta catgttttac cattaaacaa acagatagat caaaaagaac
     1741 aaaaaagaca agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat gcagtttctc
     1801 caacagggaa agtaaaacaa acaagaagcc tagagttagc aagaatatct agaaaaatct
     1861 cagaatctaa taaaacgata aggcaagaag ctcatttggc cccacaagtt tctggattgg
     1921 aaaatgaaat tttagagcct aaaataaaac aacaatcaga aaaagaattt gttgctaaac
     1981 agtcggaaga tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa cgcttgtcaa
     2041 ctattgattc caccgatgag catctgcagc cgcaagtacc acttcctagt ccgggaacac
     2101 ccgcacctgg tggaggtatt ccttcttctg ctttaggaga tttcatgcga ggtatggcag
     2161 gaacatgggt atacgctaat cgaaatgaat ttgacgcaga tgactggttc tgatttatat
     2221 ctctatctgt tatagtcaaa taagtagctg agtcatatgt tatttggcat aatggagacg
     2281 aggagggata gaaaaatgtt agataaaacg ttaccttacg cagaaatctg gatgacaaga
     2341 ccacttcaac aaaatgttcc aatcacgcct ttagcggaag gttttgaatt tcgattttat
     2401 caaaaagaag atgaatctgc ttgggcggag attgagactt ccgttgggga atttcaaacg
     2461 acagatgaag cacttgagta ttttcaacaa aactttgctc cttatcctga agaacttgaa
     2521 agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg taccgcttgg
     2581 cgaaaaaaat caagagagca agtgtatccg gtttttcatt ggttagcagt gaaaccagat
     2641 taccaaggaa aagggctcgc aaaagcattg actgccagag tcttgcaaga atttcctacc
     2701 ctccatacgg acggaccaat ctatcttcac acacagacat ggagccatca ggcgattgct
     2761 ctttataaaa aaatgggttt tacttttatt gctgaaaatc tagacggcac acctaatcca
     2821 gattatgaaa aagttatgca ggttttaagt aaattagata aataacgaaa tggtgaaata
     2881 agtcctcagt attgggataa aaaacgaaat agtcattcct catatcttac ttttttgagt
     2941 taatactatc ggataactaa tggcatgcca tacattcaaa aaagaagggg cagtgacaaa
     3001 agtattgtca ctgccccttc tcttgttttt tagcatgttt tatcgtttga tttttttagg
     3061 aataaaattc ttgatgaacg taaatttttg gtaatcgttt cgactataga ggtaaccaaa
     3121 tacactgaaa acaactgctc caataaaatt aacaaataaa tccttcatag tatccatgat
     3181 tcccacgtct aagtaaccat tttggatgat tgttttatct gttttaccat ctgcattttg
     3241 actttcaatg attgtccgag tgatatctgg aacatgatag acagtgtttc cgtctttact
     3301 taactccaca ctattgattt gctggatgat cgtatctttt tgcatatcga attgcagtaa
     3361 tttatctgct gaaaattcca caaactccca cataacacca actgtcatcg aaaaacagaa
     3421 ggaaacaact gctagtaata aaggagtcaa gttcatggtt tttgtatttt tattgagcaa
     3481 ataaacaagg gaaaagccga cacccccagc taaaaatccg ctgaatgcat gtagaaaagt
     3541 atcccacatt gaaaaatagc cataaaaatc acttaactct cctaaaactg ttgaactaaa
     3601 aataaaagat aacaaaatga tttctagcat atttggaatc tgtatcttta atatcttttc
     3661 cagaaaataa gggatttcaa ataaaattaa tgtcagtaat aaaagcattg tatttcccca
     3721 gtgttggttc cagacctgat ccactagcat aataacgacg atgatacgta ataaaatata
     3781 gattccgttt gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg ttttcaaatg
     3841 attcataaaa taactccttc atgtatatcc ttaattatac taagaagcgt gtgtttacca
     3901 aagctatatg acttaaagaa aaacagagtt gatattgtga aaaaaatcac ttaatatagt
     3961 ataaggagaa aagggggata caaatgaaaa aattagttgc tggtttatta acagccgttg
     4021 cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag acaagttcta
     4081 gtacgaagga atctgccgaa gtatcatcag gcgcttctga acaagtatat acagatccaa
     4141 aagaaatgaa agaatcttat gatgtcgtga ttattggagc tggtggtgca gggatggcag
     4201 cagctatcga agcaaaagat gctgggttga atccagtaat tttggaaaag atgcctgttg
     4261 ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca aaatttcaaa
     4321 aagaagaagg aatcaaagac tcgaatgatg ccttctatga agaaacgcta aaaggtggtg
     4381 gaggaacgaa tgataaagaa atgcttcgat actttgtcga tcactctgct gatgcggtcg
     4441 attggctaga tcaaaacggt atcaaactgg acaacttaac gatcactgga gggatgagtg
     4501 aaaagaggac acatcgtccg tctgatggct cggctattgg tggatactta gtggaggggt
     4561 tattgaataa cgtccatgag cgggaaatcc ctatttttgt caacgcagat gtgacagaca
     4621 ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca tgttcaagga gaagaaccaa
     4681 aagaggtcac cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc agcaaagaat
     4741 ttattgaaaa atatcgtccc gatttagctg attatattac gacaaatcaa gaaggatcaa
     4801 ctggagatgg tatcaccatg attgaaaaat taggtggaca aaccgttgat atggataaaa
     4861 tacaaattca tccaactgtg cagcaggatg agggggtctt gattggtgag gttgttcgcg
     4921 gagaaggagc aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa atggatacaa
     4981 gggataaagt atctgcagca attacagcat tgcctgaaaa atcagcttat ttgatttttg
     5041 accaaggcgt gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa ggatatgtca
     5101 caactggaaa gacaatggaa gaattagcag aaaaaatcga tttaccaaaa gaaacactag
     5161 caaaaacagt tgatggatgg aatacggatg ttgcggagaa aaaagatgct caattcaacc
     5221 gtgccacagc catggaacat gacttatcgt ctccgaatta ttatgcgatc aaaatcgcac
     5281 caggaatcca ttacacgatg ggcggtgtga aaatcaatac gaacactgaa gtattagata
     5341 aagaaaacca gccaattagg gggttatatg ctgctggtga agtcacaggt ggcttacatg
     5401 gaaacaaccg gatcggtgga aattcagttg cggagatcat tatttttggg cgacaagcgg
     5461 gcatacaagc agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa ataaaaagag
     5521 gttgtgaaat cagcgttttg agctaatacc gagggtcagc tgattgaaca ctgtttagtg
     5581 aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt aaaaacagct
     5641 tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg aacaccgttt
     5701 attcggaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     5761 tataaacggc ggaagcagaa gaaattcctt cggaaataag ctgaaattca caaaaatttg
     5821 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     5881 cttttttgat cagcatgatt ttattcagtt atcttcaaaa tgatttttcc gatgtttttg
     5941 tttgcttcca tatattcatg cgcttgtttg actgcgctga attggaatgt acggtcaatg
     6001 attggccgaa tcttgccatt ttctagaaaa ggcccagctt tttgcataaa agtttgtgcg
     6061 agctctgctt tatactgatc acttctaggt gtcaataatg taccaagcag ttggacacgt
     6121 ttcccaatca atacaccaag gtctatctca ggcacgatgg tcccacctag cataccaatc
     6181 aagacccaac gtccatcaac ggctatagaa gctaaattct tttcccagta agaagcgccg
     6241 atgaaatcca aaataacatc gactccccga ccttcagtga tttttcgtac ctcttcttga
     6301 aagtcttgtt ttttatagtt gatcaattca tctgcaccaa gttctttaca aaaggcgagt
     6361 ttttcttctg atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct agctaattgg
     6421 atcgctgctg ttcccacacc acttgctcct gcatggatca agacagtttc gccttcttgt
     6481 aattttccaa gccagtaaag tgtttgataa gcagtcaaaa aaacttctga gattccagca
     6541 gcagaaacat agtctagcgt atcagggaga aggattgcac gatcggcagg catcacagca
     6601 tattcggcat aaccacctag attgactaat ccgtatactt ttgtcccagg agtaaaagtg
     6661 gagatatcac ttttattctc aactacctct ccagctactt cgactcccaa aatagggtag
     6721 ggaggtttca aagagactga ttctcttttc ataatatctg ttcgattgac agctgctgta
     6781 tggactttga tcagtaattc gccttgtttt ggtttaggaa caggcacttc aatttgttct
     6841 aatacatcta cagaaccagg atgcttgatt gaaatggcac gcatggatct attcctcctt
     6901 tgcccattct tggaatgctg tattcaactc tttgctacct tcagaaagta ctagagtcgt
     6961 acgagaaata tccgtttctt ccactagagc ggcaagtaca gcagcaactg tagagcgagt
     7021 gatcgttttt gatgccttct cattaggctt agttaattga atggaagtaa cttcttcatc
     7081 gttagtcaat gtgactggtt gaagaatgac aaaatctaag ttagttcgtt tcagccattc
     7141 atctgcataa tgtttagtaa tataataatc tgtcatggac tcaggccatt ttgctggttc
     7201 atctgcataa acagcactga ccatcagata gcgagagaca tgcgcatttt cagctgcgat
     7261 cactgttttg attgcgccat ccaagtctac ttgcagaaga ttctttcctt gagagccggc
     7321 agcaaaaatc aatacatcga tgcctttgaa tgcttcggtc attttttcca cggaccaagt
     7381 taaatcaaaa gagacatacg aaacattctc tttttttact aatgtttggg aagcaacatc
     7441 tctcacccct gcaaaaattt catgggaact tggagctaaa tcttttatca ggtgtcggcc
     7501 aatttgtccg tttgcaccta caacaaaaat tttcatttgg atcatccttt cgacgtcaaa
     7561 tctttccatt caatagtaac accaaaagag aaaaaaatca gagatcggct acgatgacta
     7621 tcagttagta agagaagggt gcttgaagta tcttaacaag acattgcata ctgctgcagt
     7681 cgcaagacct gcgacgattc cttcaggtat accacttgta aaaataaccg ctagaatagc
     7741 tgaataaaca gcatttaaat ttgttccgat gactttggca taatcttgct ggaataaaaa
     7801 gtagatcata ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc cagctaagaa
     7861 taaagaaacg gaatctgctt tttgtttcat tagtttgcgc agacctttat aaacgaaata
     7921 aggaaccacc ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt tccagcttcc
     7981 atgttctgtt ccgatgactg ggataaacgg agaaaagaca aaagacaaag gtgtttggat
     8041 aactgtgcta cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag cacctagtct
     8101 tggtcctaaa acgatcgaag caatgattac aggaatatgc atggttgttg cattgattgg
     8161 accgataggg ataaaaccta aaggcgtaac agctaataaa atcaaaatag ctaaaaacat
     8221 cgcagttaat gtaaagtttt ttgtattttt cataagtact ccttttttct aagcatttct
     8281 ttcattaaga gtcagcatga cctgttcgat gatcgtctga atatcagcta aggcaccttt
     8341 tccaaaatct ccacaagcta aaagagattc tcttggttca atttcctgat agccgacaga
     8401 tctcaatgtt tccagattac gctgagtgat cgggttttga tacatattcg tgttcatggc
     8461 aggggcaatc aattttggtg ttcccactgg aagcgccatt gcgactgttg aaagaagatc
     8521 gtctgcaatg ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg cgatgagaaa
     8581 caagtcagct tgttttgcta gttcgatatg attgattacc tgcggatttg attcttgcat
     8641 gacgtctgta tggactgcgc gcttcgacag agattgcaag gttaatggcg tgataaaatg
     8701 tgtgctgctt ttagacataa tcacatctac ctgggcacct aatttgacga attgacttgt
     8761 aagatcagcg cttttgtaag cagaaatact tccagtaacg cctagtaaga tctttttcat
     8821 tttgattcct ccagtctaat tttctcagta atcaatgcag caatctcaga tttactttga
     8881 gcttcttcca ctgttccatc tttagataag agatagccgt ggtgtgtctc gccatggatt
     8941 tccgtcaaat catttgcgaa aataaaatca gttttgttat ttatcagact attttttgcg
     9001 actgccagaa gagattcctt tggtacatct actaatagtt tgaaaccaat gatttttgct
     9061 tttggctgcc attcacgtag ataagaaatg atttttggtg ttttcttcaa aacgagcaca
     9121 agatgctcgg tgttagaaga gatctttgaa acagtggtat ctgtctgttc attttccgca
     9181 aaccaattgt ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc cagctgttct
     9241 tctgaaaaac taaaagcagg agtaaaatca ctgactgcca tactgtggat aatcgcatcg
     9301 tagtgttctt ttttcgttag ttcttctaat tgtaaaaata agtccagtgc agattcgatc
     9361 gtataaatcg ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt agtcacgtaa
     9421 tcgattgtgg tatccggatt ggcagctaaa taatcagcta atgcttgccc taatcgtcct
     9481 gtcgaatgat ttgtaatagc acgtacttga tcgatttttt cagtcgttcc tccagctgtg
     9541 attagaatct tcatttatgg tcacctacat ttactagtgt tcttccttgg tgtgttccag
     9601 caagtagttt agatgctgtt tcaggcaatt gatccagagt aatctcttgg acgaccagtt
     9661 catctgcaat ctgcaaatcc gccatctgtt gccataaaga aagacgttga tctattggta
     9721 cattgacaga atcaattcca atcaactgga tgtttcgcag aataaaaggc aagacagtcg
     9781 cgtttagttt gattccccca gcattgccac ataaagctac tgcaccgtta taagagatca
     9841 atggaagaag actgcttagc tgctcgccac ctactgtgtc gatgacatag tcgatttgct
     9901 gttttcctaa agcttttgtt gtctctggca gaaattcatc aggtcgtacg acttgcaaag
     9961 cacctagact ttttagccat gtgacagact cttcttttct ggaaaaggta atgattgact
    10021 ggaatcctag tttatgtaac agggctatcg ctgtactgcc tacaccgcca gaagcaccag
    10081 taacgacaac ttttttatcg gaggtcatac cttgacggat taaagcgttc acacacaaca
    10141 tagcagtaaa ccctgctgta ccaaggatca tcgcttgacg cagcgtcata ttttttggta
    10201 gaggaactaa ccagtctcct ggtacttttt gatattggct ataaccgcct ggatgggtga
    10261 cgccaagccc aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt ggattgttac
    10321 tttggacgac agttccggat aaatcaatcc ctggagtcat cggatatgtt cgtatcacct
    10381 gaccagactc gctcattgcc aatgcatctt tataattgac atcagaatag gcaattttta
    10441 ccaagacttc attttcagcc aactcagtaa atggcagatc actgattgct gtatggaaaa
    10501 caggttcctt tattacctga aaagatcgaa aagcgttcat tcgtgagaca ctccttttaa
    10561 aattttcttt tatcatacca taaagcctac tcttttattc ttgttcttcc gtaaatttag
    10621 acaaaaatta ggtgaaaaat aaagaatcaa acaactagaa tttattccaa aagataaaga
    10681 aatattcagc aaaaaaaatt ttacaaaggg ttgccttttt caataaattt cgtatactaa
    10741 tacgagtggt agtaaacagg aagtttacta ttagaaaact tcgattcgga ggaaattatg
    10801 gaaattggtg agaagttaag aaatttgcgc atccaaaaga atttgacaca agaagaacta
    10861 ggcgaacgca cagatttgtc aaaagggtat atttcccagt tggaacgaga tttaagttca
    10921 ccatcaatgg aaacattctt ttctattctt gaagtcttag gtgtgactcc agaagaattt
    10981 tttcatcaag aagcagcgaa tctgcaagtg gtttattcaa aagaagatca tacggtttat
    11041 ttcgatgaag aaaacgggta tgaattggaa tggctcgtgg ctgattcgaa tgaaaaagaa
    11101 atggagcctg tcttgctgac ttttgaaggt gcaggagaat ataagacgtt tgaaccttca
    11161 ttatctgaaa cgtttatttt tgtattagaa ggggaattag agttaagctt aggagagacg
    11221 gtctatgtgg caaaagcagg acagtcgatc tattatcaag cgacaaaaca acatcaatta
    11281 aggaatcatc tcaaaaaacg aacaaaagta ttgattgttg caacgcaatc atatttgtaa
    11341 tatagggggt aaaaacgtgg ggaaaccaat catttcattt gatcaagtag tgaaacgcta
    11401 tgatgatgaa acagtattaa aaaaagtaag ttttgagatc gaacaaggaa aattttatac
    11461 actgcttggt ccttccggct gtggtaaaac aacgatccta cgtattatag ctggattcac
    11521 cgaagctaca gaaggggaca tctattttga agggaaacgg ttgaatgatt tgccagctaa
    11581 taagcgacaa gtaaatacag tcttccaaga ttatgcactt tttcctcata tgaatgtttt
    11641 tgataacgtt gcttttggat tgaagatcaa gaaaatgtcc aaatcagata tcgaaaagaa
    11701 ggtaaaagat gctttgcgta tggtccagct tcctggatac gaaaaacggg agattagtga
    11761 aatgtctggt ggtcaaagac agcgtgtagc gattgcacga gcaattgtaa atgaaccgaa
    11821 agtattgcta ttagatgaac cgttatcggc gcttgatttg aaactacgta cagatatgca
    11881 gtatgaactg cgggatctgc aacaacgatt aggaatcacg tttatttttg ttactcatga
    11941 ccaagaagaa gcgttggcaa tgagtgatga gatttttgtc atgaataaag ggaaaatcgt
    12001 ccaaagtggt acacccgtag atatttatga tgagccaatc aatcatttcg ttgctgattt
    12061 tgttggggag agcaatatcg tggacggtgt aatgatcgaa gataatctag ttgagtttgt
    12121 tggtaagcaa tttgaatgtg tcgatggcgg gatgcgtcta aatgaaccag ttgaagttgt
    12181 tttacgccca gaggatttga cgatcacgac accagataaa ggaaaactag tcgtaacggt
    12241 cgatactcag ctgtttcgtg gtgtccatta tgaaatcatc tgttatgatg aacagcaaaa
    12301 tgaatggatg gtccattcaa cgaaaaaagc taaagaagga agcaaagtag gattggcctt
    12361 tgagccagaa gatatccatg tcatgcgttt caacgagtct gaagaagaat tcgatgcacg
    12421 tcttgacagc tacgaagaat aggggggaca agaatgaaaa caatgcgcag gatctattcc
    12481 gtcccttata ttttgtggct cggtttattc gtgatcgcgc ctgtattaat gatcatttat
    12541 caatcttttt ttgatatgaa tgggcaattc acgctgaata attatctatc ttattttacg
    12601 tcggggacat cgctttctat gacgatcaat tctgtatggt atgcattttt gatcacgttt
    12661 ttcacgctga tcatcagtta cccgactgct tattttttaa caaaactgaa gcacaagcaa
    12721 ttatggctga tgctgattat tttgcctaca tgggtgaatc tgctgcttaa agcttacgca
    12781 tttattggga ttttcagtgt gcacggcagc gtgaatcatt tctttgattt tatcgggatc
    12841 gggccgaaac agatattatt cacagacttc agttttctgt tcgtggcaac gtatatcgag
    12901 attccattta tgattatgcc aattttcaat gcattggaag agatgaatcc ttctttgatc
    12961 agtgctagta gagatttagg cgcgaatagc tttgagacct tccgacgagt gatttttccg
    13021 ttatcattaa atggtgtgaa aagtggtgtc caagcagtat ttattccttc tctttcttta
    13081 ttcatgctga ctcgtctgat tggcggaaat cgggtgatca ctttaggaac ggctatcgaa
    13141 cagcatttct tggttacaca aaactggggg atgggttcta caatcggtgt gattttaatc
    13201 attgctatgt tcatcgtgat gctcctgaca ggcgagaaga aaaaaggagg tcgctcataa
    13261 atgaaaaaat ttaaatggtc gtatctttat ttagggatcg tctttttatt cttatatgca
    13321 ccgatcttct atctgatttt ttattccttc aatgaaggag gaacgatgaa cagctttaca
    13381 ggatttactt gggaaaatta tgaagctgtt tttgaagata cacgtttgat cattatcgta
    13441 ttgaatacgt tcatgctggc tttcttatct gcattgatcg ccacgatcat cgggacattt
    13501 ggggctatgg gaatctatta cacgaaaaaa cgacgtgcac ggacagcgtt gttgagcatg
    13561 aataacatct tattagtctc tcctgatgtt attatcggtg ctagtttctt gattttcttt
    13621 acactactag gattcggatt aggttttcag agtgtattgc tttcccatat tgctttcagc
    13681 attccaatcg tcgtcttgat ggtcttgcca aaattgcagg aaatgaacga ttcaatggtc
    13741 gatgcggcta gagaccttgg tgctaataat ttccaagtag tcaaaaatat cattttgcct
    13801 tatctttctc caggaatcat agctggatat tttatggcat ttacctattc tcttgatgat
    13861 tttgcggtta catttttcgt cacaggaaat ggctttacga cgctctcagt agagatctat
    13921 tctcgtgcaa gacaaggaat cagtttagaa atcaatgcgc taagtgcgtt agttttcttg
    13981 ttctccatga tattggtcat tggatactac tttattagta aagaaaaccg tccaaaacgg
    14041 ctgaagaaaa acagaagagt acgcgagggg gtggcacggc tgcgatgaaa aaattacaat
    14101 ctctgttttt aggtattctc atcattattg cggtgctgct gcttggtgtt cgtcaacttg
    14161 aaaaaacaag tggcatgagt ggagcaaagg tacttaccat ctataactgg ggagattata
    14221 tcgatcctag tttgatcacg aaatttgaaa aagaatatgg atacaaagtg aattatgaaa
    14281 catttgattc taatgaagcg atgttcacaa aaatccagca aggtgggaca aactacgaca
    14341 tcgctatacc aagtgaatat atgatccaaa aaatgatcaa agaaaaacta gtacttccat
    14401 tagatcattc gaagatcaaa ggattaaata atattgatcc acgattttta gacttggatt
    14461 ttgatccaga taatcagtat tcgattccat atttttgggg aactttaggg atcgtatata
    14521 acgataaatt ctttgatgca aatcaaatta agcattggga tgatttgtgg aagccggaat
    14581 taaaagacag tttgatgctg attgacggtg cacgagaagt tatggggctt tctttgaata
    14641 gtttaggcta ttctttgaat agtaagaata tggcacagct gacagaagca gcaaataaat
    14701 tgaatcattt aacaccaaat gtcaaagcaa tcgtggcaga tgagatcaaa atgtatatga
    14761 tcaatgaaga agcctccgtg gcagtgacat tttctggcga agctgccgat atgatgtggg
    14821 aaaatgaaca tttacattat gtgatcccac cagaaggatc caatctttgg tttgataata
    14881 tcgttatgcc aaaaaccgtg aaaaataaag aaggtgccta tgatttcatc aactttatgc
    14941 tagaaccgga aaatgcggca caaaatgcag agtatattgg ttattctaca ccaaatgaag
    15001 aagcaaaagc gttattgcca aaagacattt cgtcggacga acaattctat ccatctgatg
    15061 acacgatctc ccatcttgaa gtctacaaag atctaggatc gaaataccta ggcatctata
    15121 atgacctatt cttagaattc aagatgtata ggagataatg gcaaaactca gtaagaatgg
    15181 ggacttatca acgttctaaa gagctaaaaa tcgtcctttt tctatctgag ttttccaatc
    15241 ttctaccaaa agcggatgaa acgccgcttt ttttgtgtta ttttattttt tctaccacgt
    15301 ttttctacca gagaaaagtg gacaaaacca ttgtaaaata gggcccgaga cgggctagta
    15361 aatctaagat tgaaaaagtt acagaaggat aaagtaattg gaaactatgc aaatatactt
    15421 aac
//
LOCUS       DAZKMB010000065        15260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000065 DAZKMB010000000
VERSION     DAZKMB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.065"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG8V3_001788"
     CDS             <1..878
                     /locus_tag="ACG8V3_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0300323.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
     gene            complement(1119..2735)
                     /locus_tag="ACG8V3_001789"
     CDS             complement(1119..2735)
                     /locus_tag="ACG8V3_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0300324.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            2853..3071
                     /locus_tag="ACG8V3_001790"
     CDS             2853..3071
                     /locus_tag="ACG8V3_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300325.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3266..3727
                     /locus_tag="ACG8V3_001791"
     CDS             3266..3727
                     /locus_tag="ACG8V3_001791"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0300326.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3920..4159)
                     /locus_tag="ACG8V3_001792"
     CDS             complement(3920..4159)
                     /locus_tag="ACG8V3_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300327.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4183..5706)
                     /locus_tag="ACG8V3_001793"
     CDS             complement(4183..5706)
                     /locus_tag="ACG8V3_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0300328.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(5724..5846)
                     /locus_tag="ACG8V3_001794"
     CDS             complement(5724..5846)
                     /locus_tag="ACG8V3_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0300329.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(5876..6859)
                     /locus_tag="ACG8V3_001795"
     CDS             complement(5876..6859)
                     /locus_tag="ACG8V3_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0300330.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="ACG8V3_001796"
     CDS             complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="ACG8V3_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0300331.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(7053..7430)
                     /locus_tag="ACG8V3_001797"
     CDS             complement(7053..7430)
                     /locus_tag="ACG8V3_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300332.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="ACG8V3_001798"
     CDS             complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="ACG8V3_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0300333.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="ACG8V3_001799"
     CDS             complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="ACG8V3_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0300334.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(8448..10214)
                     /locus_tag="ACG8V3_001800"
     CDS             complement(8448..10214)
                     /locus_tag="ACG8V3_001800"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300335.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(10174..11928)
                     /locus_tag="ACG8V3_001801"
     CDS             complement(10174..11928)
                     /locus_tag="ACG8V3_001801"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300336.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(12038..12607)
                     /locus_tag="ACG8V3_001802"
     CDS             complement(12038..12607)
                     /locus_tag="ACG8V3_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0300337.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(12625..14040)
                     /locus_tag="ACG8V3_001803"
     CDS             complement(12625..14040)
                     /locus_tag="ACG8V3_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300338.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(14051..14719)
                     /locus_tag="ACG8V3_001804"
     CDS             complement(14051..14719)
                     /locus_tag="ACG8V3_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0300339.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(14849..>15260)
                     /locus_tag="ACG8V3_001805"
     CDS             complement(14849..>15260)
                     /locus_tag="ACG8V3_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0300340.1"
                     /translation="TSFFVWIFSFTQNILHSQSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT         4902 a         2915 c         2211 g         5232 t
ORIGIN      
        1 tttctttgag ccagctgtta ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa
       61 atgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctataaaag
      181 tccgtgaaca agtgcgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacattg
      301 gtcgggcttt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
      361 ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gattccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgttat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatac caaacagaaa
      661 tcaaacgagc gttgcgcgtt ccctattcca acggccctat agaatgtttg aataaccata
      721 tcaaagtact aaaacgaatt gcctatggct atagaaactt tcaaaactac cgagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa tactacccaa ctgactaaag
      841 ccagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcttcaccaa cactcattga cgaagagcca aaataagtaa tctaaccgac
      961 tgttaactaa accattgcag gtcccgttaa agatacttgt gcctgttgtc accgcttctt
     1021 tttcttgtcc acaagcgctt aacacactta ataaagcccc tgctattatt aataataata
     1081 tctttttctt attaaaaacc tcctcatcta tttccagatt agtaaatgta gtaatctgtg
     1141 ctttcttttt tctcaatact aataactaac ttatggggta aacaaatact cgtttgaccg
     1201 ggttttgaga tccatccagt tctaaccgcg atttgatcag gactattatc ttctttaact
     1261 cgaattcttc catctttaat ctcaacaata ttatattgtc ccttcgcagg atgataattc
     1321 acgagtttat gattgatctt atccaaatcg aagcgatcca tttccttgcc atcaattcga
     1381 acgatggcat atttgatagc atcttcagtt gtttcattct ccttttctat agagaataca
     1441 aataagggaa taaaagaggt aataatcaaa atgccaataa ttatgacatc gcctatcttg
     1501 acaccagctt ttcttaaaaa tttcattaac gatatctggc taaacatatt agactttttc
     1561 catggaaatg gtgaaatttt tcggaaatta tctgcattaa gagaatcagt taaacaaccc
     1621 tcaagttgtt ccttaataca ttgttgatcc atcttcatac caataaaaac tagtttatta
     1681 atgcgttcac caaacaacgg atgccaggct tccttcaatt cgggattttt ttgtataatc
     1741 atttgacact caattttagg taaactagct acccaataat ttaatgattc aatactttta
     1801 tagctacccg atgtttcaaa gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac
     1861 ccttttgctc ttgtaatatt gccaggaaaa tttttatccg taagatgggc aaatttttgt
     1921 gcgtcaagga gtaaccgatt tcgatagata aaacttgaga taccatattc ttctgtttca
     1981 ggaatatgat tttgatagcc aagctctaac tctttgaacc agcccactga ataggaagtc
     2041 tcatctatat taaaaagatt tgtatttaga attttcgata agtctacttc acctttaatt
     2101 gtcctaatta ttttggcatt ggactgtaac ttagcaatta gaccgttaat tttgtaaatc
     2161 gtcttggttg acactaagtc actattattt aaaagtaata tgttacaaca ctcgaattga
     2221 tcaattaaca agccacgaat atcctgttct tcttcaacta cattaccttc agcttttctt
     2281 tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac gttttacatc cactactata
     2341 ataatggcac caatctctaa tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat
     2401 ataattgctt gaataattgg aattggtttg ctgattcctg aagcttcaat aacaatttga
     2461 ttcaagtcag aaaattgcac taattgatct agttcaacaa tcaaatcttc gcgtaatgta
     2521 caacaaatgc atccattcgg catcgaaaca agtttttctc cagttctttt aaaacctttc
     2581 ttgtcgacta ataccccatc aacatttact tcccctatgt catttacaat gacagcaatt
     2641 tcctttccgt gatcaccgta cagaatatga tttagaagta tcgtttttcc tgaaccaaga
     2701 tagccagtta agattgtaat aggtattttt ttcataggga acctctttct ataccttgaa
     2761 tttacacttt aagtgcaaca cataaaaatg agtcataatc gatatccaac tagtataagt
     2821 ttgtcgagaa cactcctaga aggaaaataa ttatgagcca gtatcgtcat cttactatac
     2881 tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt ttctattagt ataattggca
     2941 acgacattca gcatagcccg tttactattt tacatgaact gagaagaaac aagaaaaaaa
     3001 ctattcacct tccaaagcgg aaaaaaatta tgcttatcga agaaaaaaat gcggacgtta
     3061 tcacattcta aatgatccgt gagtttggtc aattgttcgt catttatttg tcgattttca
     3121 gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa catatggact tttcaattag
     3181 ttttatcacg atctaacgtg caatttgcaa tgatcttttc gatacagaac ccctctcaca
     3241 cggcaataaa ggcctgatcc gttcattgca ccatcgagga aaaacaagac atacaaaaaa
     3301 tcatgtcgag tctcgtggaa agatttgcct tactcattcg atccatggcc gtccaaaatc
     3361 cgcagatgat agaagtgatt ttggtcattg gaaagccgat attgtagctg gcaaaactgg
     3421 tggtacttgt ttagtgactt tgacagacag aaaatcatgc ttccttttag caggaaagaa
     3481 ggtccccaga aagaaagccg cttatgtgag cgagaaaatg attgaacttt taagcagtat
     3541 tccaaatgag gcggtcaaaa cgatcactcc agatagagga aaagaattct ccaatcattt
     3601 cgcagttaca gaagcattga ataatatgcc cttttatttt ccagatcctc atgcccttgg
     3661 caacaatgaa caaattaaaa tacgaataga ctcattcgag aatacttgcc aaaatcgaaa
     3721 gagatagcta cggtagataa ctcaacgata aatcagatga ttgaaaaact gaataacaga
     3781 cctagaaagt gtttagattg gagaacacct tatgaagtct tctttgataa agtgttgcgc
     3841 ttgatttgac aattcaagac ataataaaaa gcagatcaaa tccaaataag ggaaaaatga
     3901 tctactctgg tattatcaat taattggcca agctattatg ccgttttgct aaacgtctac
     3961 ttcttccagt attgaccttg ataaaacaac aatcaggatc ccgttttaat actctatgaa
     4021 ctgtgtcaat actaacatgt aaagttgcag caatatcagc taatgtactc ccatttaatc
     4081 tcatttgctt tgcaaatcga taacgccgat aagcatcaat attcttggaa tccatagaca
     4141 ataatacttt tcttaccatg tatttcctcc aaaaattgaa ttttaatgag ccatcatttc
     4201 ttgagcaatt tcatgtttag acaatgaagc tggatagaaa gtaggccaat tatgcaattc
     4261 attatagagt ttttcttgac tttctccacc aaagaaaata tggaaatgct ctgccttact
     4321 tggtgcaatt ccatgatcac taaattgaat atatttaaaa cgaccagcac ctgcatcatc
     4381 agtttcaaaa ttaaaacgaa caccacggtt tcctttttca taattcaaaa ttttgtatcc
     4441 aacatatttg tatgtgtatt gatgatgttc accgttgact acaaaatcaa tggtgttgtc
     4501 tttaatgttg atagtatcaa tatctgtctt atagccagta gtataataat ctttgtactc
     4561 ggcagctgtc atgtctttat tgatttttgc tttgtaatcg aaaacttcat ccaaaattcc
     4621 atcttttaac aacggataaa cagattgcca ttcgcctgta taatcactca attcgcgatc
     4681 cttcacatcg gcatctttaa agtacccatt agcaactgtc ttttcatcct tcgcttcttt
     4741 ttctggaact acctcttcac cagcaaccat agttgttttt tctaacgctt ttagattgtc
     4801 ttccattact gaaacataat cttcaccatt atccatttgt tcttttgtaa gactttcaag
     4861 aggattcagt acttccaatt ttatcccagc ttcgttggcc aaagtttttg caattttatc
     4921 attagcattc ttttcaaagt aaatataatt gatcttattt tttttaacat actcttttaa
     4981 ttcagctaat cgaccagaag atggctcttc ttcaggactt agtccagcaa taggaacttg
     5041 tttcaatcca taatccaaag ctaaatatcc aaaggctgcg tgttgcgtca caaaactttt
     5101 ttgtttcgct tctttaagac tagtggtata gtcagcatcc aaccgtttca gtttagtcaa
     5161 atacttttca gcatttgtct caaatacttt tgcttttttc ggatacaatt taactaactg
     5221 atccttgata tttgaaactt ccttaattgc cattttgggt gaaacccacg tatgaggatc
     5281 tagttcatga tggtgatctt cttctccgtg atcatgatct tcttcttcac tacctggcaa
     5341 taaaatcatg tcttttgttc cttctactac attaggccca gctttcttcc aactttcttt
     5401 agcttttggt acccatgatt ccatgttttc gttatgatat acaaaaacat cagcatcatg
     5461 agcttttgcc atatccttag ctgagggctc atagtcatga ggttctgatc ccgctggaac
     5521 cattaaatcc acgttcgctt catcgccgac gatgtttttg gtgaaatcat acattggata
     5581 gaatgttgtt acaatttcta attttccatc tttatcattc gatacttttg catcattagt
     5641 gctggagcaa gctcccaata atcctagagc tgctatccca ataagtccta cactgatttt
     5701 cttcattctt tttccttcct taattatgct aatttcttca ttttatttcg tttatgttct
     5761 ttgataattt ttaatgccct ttttctagtt ttaatattcg ggcttttaag tcgtcgatat
     5821 gcactagata tatccgtttt ctccattaga aattaccccc aatcactact tatcattatt
     5881 tgctttccgc cagtaattta tcaaaactct gtttgatcct ttcttcatcc aacatttttc
     5941 cgattagaat aatagtggtt tggtcttttt gtcgagtggt gtattgaaaa ttaacctgtt
     6001 cgtttacctc ttgcatggca actataaaat cctgctcttg taaagcaatc aatcctttaa
     6061 aacgatacag gctcccttgg ttggttaaaa tcagccagtc aatccattgg agtaaaaaat
     6121 ctttttttat gtcttgatct gaagttaaga gaattgattt aaactcatgt acttgatggt
     6181 gttgatgata gtatttgcca ttatagtgat tttctgatga atcactttca aaaactaagg
     6241 gatgattaaa ttttttcaaa ttataaaaat cctttttttg aatcgtttca ctatgtgtga
     6301 acattcgaat atcggataaa gggttaatac ttttgatttc tctctgtaat ttttttagtg
     6361 cagataaatt caagttttgt tttctagaca caaaaatgcg atctgccatg acaagttgct
     6421 taagggcttg tggttctctt tcaataagtt caaaactgtc actatcaata acagtcaata
     6481 aactatcgat atatactctt cctcttagtt gaggcgctgt taaaagtgtt tgaatgattg
     6541 gttggggatc tgctatacct gtagtttcaa tgatgatttg tgaaattggt tgttgatgtt
     6601 cttctattgt ttccagtaaa gaaatcaata ttgctaataa atcactgcga agactgcaac
     6661 acatacatcc attatttagt tgtaacaccc gttcctcgct atgaactaat aatttgtgat
     6721 caatcccaac ttcaccaaac tcattttcaa ttataataat ttcttccctg gggtagggtg
     6781 agacttgcaa ggcttgattg attagcgttg tttttccagc ccctaaaaac ccagaaacga
     6841 ttgtaactgg tatgcccatt ttgtcacctt tttaccactt agctatttca cttctttaaa
     6901 aattgcccgt ctgcgcaatt ttggcgaata cttttttaat tctaaccgtt cagggttatt
     6961 ccgtttattt ttgctggtga gatacagacg ttctccagtt tcgacacatt ccaaaataat
     7021 attttgacgc ataaatcttc ctccttcaat acttatcgat cttcaagata tgcttgaatt
     7081 tgttgtgcat cttcaaaagt taactcgcca gattccaatt gtgaaactaa attttctttc
     7141 atatcaacag gtatctccca ataaccatcg atacattgaa ataactcctc gtcatactct
     7201 tcaaaaattg gatagattct taaaaaatca tcaaatgata agccttggtc ctcatagtaa
     7261 tctttgacta atccattttc aattgcctgt tgggtcggta aacccgtttc agtaataatt
     7321 cctctctcta ccatcgaacg ataaaaagtg catttatcta gcgattgctg ggtcgtttca
     7381 agataatact ttaatccttc ttcaactaaa ggtgggtaaa taatttccat tttgattccc
     7441 tcctaaaagt tatatattaa actattttga agctccttta attactttct ttccattata
     7501 gtaccctttt aaagatacat gatggctacg tctataatca ccattcgctt cgtcaaatga
     7561 aatttctggt tttactacag tttgatgagt tcttcttaac cgtttcttcg ccttagatgt
     7621 ttttcttgct ggtactgcca tgatatttcc tccttttacc aacttgcttt ttttactcca
     7681 ggaatcaacc cttgatgggc aagttctcta aacttaatac gggacatacc gaactttcgc
     7741 atataccctc ttggacgacc atccgtctga tcacgaagcc tcagacgatt cggatttgaa
     7801 tctcttggca atttagcgag agcggtgcga tctcctgcgg cttttaattc ctgacgttta
     7861 tctgcatact tttcaatcat cgccatttgt ttctttgctt tagcaatttt cgactttttt
     7921 gccatatacc tttatactcc ttttattttg atttagacac ctcaaatcgt aatcgttacg
     7981 ttttgacact gatactttac caacaagagt tgctcttgtc aacaataaat aacatgaaat
     8041 aaaactcata tgaatagata actatttgaa aacgaattta tcggtacata gagataatca
     8101 aatcatttaa aagaatatgc agtttaggct ctgaaaaata agaattcact tttattagtc
     8161 caatcaaaca caaataccgt tttaagtagc ttttcctcta cttaaaacgg caatatgaaa
     8221 gaaactataa ctttttattc aatcttttat gtttaactgg tttatcgcca attttttaaa
     8281 actttgatta ttccttatat tataagaata gacattaagt gaactaattc aatcatactg
     8341 attctttcgt gtattcaaaa ttagatgctg tttaataaaa ttattctttt tgaaaaagaa
     8401 ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca tcgtgattga
     8461 gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag atttttgtat
     8521 atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt tccttgatcc
     8581 atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat aattagtact
     8641 gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt ttccgcatct
     8701 aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa agcccgtgct
     8761 atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc gaccaatgta
     8821 tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg agctgctttt
     8881 ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa aatacttgta
     8941 ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga agctggagat
     9001 acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc atacagtcta
     9061 gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa cgcagtcact
     9121 tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt atcatatgag
     9181 aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga tccctcttgg
     9241 atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata aaatatttca
     9301 agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc tagtaagtaa
     9361 ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc tactagaatg
     9421 actactgcta aactaacata tgagaaaata gaggataagg ccatgactga aaaagtactt
     9481 aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag tctgtcttga
     9541 acttttttag atagattaaa gctattaatc tcttgctgca attctatagt ttcttgaaat
     9601 tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt ttgggatttc
     9661 tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc taatcccatt
     9721 ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc cataccgccg
     9781 gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga catgatagtt
     9841 tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt tgacaaatcg
     9901 tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata taagttttca
     9961 tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac ggcaaaataa
    10021 agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat tataatgaat
    10081 gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt agttaaagct
    10141 tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat tgtcaagcct
    10201 ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat gtttagccat
    10261 taattcatta tgatttccac gttctactat tttcccttct gacaagacaa gaatttcatc
    10321 tacagattta atgctggata atcgatgagc tatcataata actgtttttt ctttcatcag
    10381 atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg cactggcttc
    10441 atccataata acaatctttg catcttttaa aattgctctg gcaatagcta tacgttgttt
    10501 ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc tttcgggaaa
    10561 tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt tacttgctgg
    10621 cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata gtttcgtatc
    10681 ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat ttaaaggctt
    10741 accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta gtttagctat
    10801 cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt gttctaaaac
    10861 taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat tttcaaatgc
    10921 aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt gcttcatttc
    10981 taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt gcatacttac
    11041 atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca acatcaacaa
    11101 gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta taggaactat
    11161 caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact
    11221 caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca aggctttaaa
    11281 tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat attctaccgt
    11341 ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg tattctctgt
    11401 cattttcttt aacaagaata caccaattac taacatggcg attagaacca atccaatttt
    11461 aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg cttgagcact
    11521 atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat caattgtctt
    11581 tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct
    11641 aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag aaaataagcc
    11701 agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa ccgcataact
    11761 cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt aaatactgta
    11821 atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta accatccgta
    11881 gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt tttcctcccc
    11941 cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta atagcgtaaa
    12001 ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga
    12061 cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac actgaaatga
    12121 ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca gattgtcctt
    12181 tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc cactgtccgg
    12241 cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct cgatcatatc
    12301 caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg ataaaccaag
    12361 gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg taaatagtaa
    12421 aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca gataataatc
    12481 caaaaatccc gggggatatg ctgtgaccaa acgccccacc aatactacta accattattg
    12541 aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaata taatcttttg
    12601 ttttcatata tattctcctc aattctattt cgttatgtca ttaaaaatct gttctatcaa
    12661 agttgaatct tctactctaa gttttatatc tttttttatt ccaccatttt ttaaataaat
    12721 aattctattg catgttttat aaagaaattc cggatcatga gtagctataa ctactgcacc
    12781 cttttctttt aagtctttaa taaggtcagc aatcctttcc atattaatat aatccaaccc
    12841 acttgtaggt tcatcaaata gaaataaatt agtttttgac aatgaagcta tagaaaccaa
    12901 taatctttgt ttttctccac cggataaatc aaacggtaat tgatctttca ttttataaag
    12961 ttccattttt tttaaaattt tttcttgctg atctttacta actatttcat tccccagggc
    13021 taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc atttcttgca ttactaaaaa
    13081 cggcgatgac aaattttttt tattttcgat tatctttcct ttggtagggc gaataatctt
    13141 catgagtatt cttagtaagg ttgttttccc cacaccgttt ttccctacca aaccaataac
    13201 atctccaact ttgacatcaa aatttatgtc ttttagaata ttttcataag taatttcttc
    13261 aacttttaat acatctgcac ttaccttttc gatataggga acttcaatcg aactattaaa
    13321 aatgtcaaat ttccttaatg cgttattgtt ttcagttttt aacaactgaa tagttgtagt
    13381 ttttacagtt ctatttttaa caaatagaac ttgatcaatt aattctttca aatagtaaaa
    13441 acgatgttca gctataacaa tagtaaatcc tttatttttt aaatcacaaa gtaagtcagc
    13501 aagtttctta gtcgctttga agtccaaatt tgaagacggt tcatccaata taattaattt
    13561 agagtttagc gataaactac atccaataga aattttctgc cgttcaccac ttgatagcga
    13621 aaaaatgttt ttatctaata tatttgaaag atctaataat tcagctaatt cattgattct
    13681 tttatccata tgctcttttg taaatccaaa gttttccatc gaaaatacta actcactcgt
    13741 tgtatttgtt gtaaagaatt gtcctctagg atcttgactt acgtagccaa tattttttga
    13801 tatactttct tgtgttaagg attgtagcga gttattttca aataaaattt ccccaaccaa
    13861 ttttccgtca taaagtcttg gaataagtcc attcattaac tttaatagtg tggatttccc
    13921 actcccagaa gaaccagtaa tcaatgtaag actgcctttt tttattgaaa aattaaggtc
    13981 aacaagcgct tgttccttaa catccttata tgaaaaagaa acattgttaa attcaaacaa
    14041 ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt aacggaaaac aggatgatat
    14101 agtctaataa gctcatgttt gttttatagt aagaacttct tttcacagga gattctatcc
    14161 cctttgttat tgcattagcg gtcatttctt cagcaatcat agttgcacgt ataagaacgg
    14221 gaactaacag aaattccaaa tattgcagag gctgaaaaaa agacaagcga atgtttctca
    14281 tttttaaaga ttctttaatc attcttattt cagaacgata agtcggaaag aatctgatca
    14341 ctactgtaat acttaacatt atttttttcg gtaaatgtat tttttctaaa gaggataata
    14401 attcattgct agtatagaca ctaaccaaca accatgagat cattacgacc ggcaacattc
    14461 gaaatacaat atataaggat gtcgcaaaaa atatagtaaa aaactctatc ttgatagcat
    14521 caataatttt cattactaaa aacagaactg aataaatcaa gatgaaataa atacatgtct
    14581 ttattttacg aaacatcaat actaaaactg tagcaataat gaaacacccc acttgaaata
    14641 tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat atttaaagta atcaaggtta
    14701 acggattgat ttttgacact ctttcacctc caaaaaataa gatcgcactg ttatcttaag
    14761 ctgataaaaa tgcaccgtta ttcagataac agtgcgatct tatcaaaaag cataactgag
    14821 aacaattctc aatgcaagct ttttttaatt aactcagtaa ttcaatccac ccaggaacag
    14881 aaaattgata caatcttctg gttaatagca tagtcttttc ttttgaatag ctatgcttca
    14941 atatatcata gaatgtagcg tagaatgagt aaatgtagaa gtggatttct tcttcattaa
    15001 agactaaatt ttgattgatc attccgatag tctttaactt atctagacaa cagactgtct
    15061 ttttcgtcac aaatgatgta attttttcaa caatatcttg atatttggtt ccagctgatt
    15121 ggaataaaat caaatcgaat atacgtttat tctcaaaaat ataattaata aatggatcta
    15181 attcattgaa gttctctctc caactttgag agtgtaaaat attttgtgta aatgaaaaaa
    15241 tccatacaaa aaaggaagtc
//
LOCUS       DAZKMB010000066        15255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000066 DAZKMB010000000
VERSION     DAZKMB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.066"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1021
                     /locus_tag="ACG8V3_001806"
     CDS             2..1021
                     /locus_tag="ACG8V3_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0300341.1"
                     /translation="MSIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLIT
                     GKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFF
                     TSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMI
                     ISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSV
                     FLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNIL
                     LFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTV
                     RRLKR"
     gene            1018..1785
                     /locus_tag="ACG8V3_001807"
     CDS             1018..1785
                     /locus_tag="ACG8V3_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0300342.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8V3_001808"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8V3_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0300343.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="ACG8V3_001809"
     CDS             3750..4070
                     /locus_tag="ACG8V3_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0300344.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="ACG8V3_001810"
     CDS             4070..4420
                     /locus_tag="ACG8V3_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0300345.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8V3_001811"
     CDS             4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8V3_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0300346.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8V3_001812"
     CDS             5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8V3_001812"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0300347.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="ACG8V3_001813"
     CDS             6414..7439
                     /locus_tag="ACG8V3_001813"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0300348.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="ACG8V3_001814"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="ACG8V3_001814"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0300349.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="ACG8V3_001815"
     CDS             8517..8957
                     /locus_tag="ACG8V3_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0300350.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="ACG8V3_001816"
     CDS             8960..9562
                     /locus_tag="ACG8V3_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0300351.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="ACG8V3_001817"
     CDS             complement(9625..10728)
                     /locus_tag="ACG8V3_001817"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0300352.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8V3_001818"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8V3_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0300353.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="ACG8V3_001819"
     CDS             12294..12689
                     /locus_tag="ACG8V3_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300354.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="ACG8V3_001820"
     CDS             12689..12940
                     /locus_tag="ACG8V3_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300355.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="ACG8V3_001821"
     CDS             12983..13378
                     /locus_tag="ACG8V3_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300356.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="ACG8V3_001822"
     CDS             13375..14631
                     /locus_tag="ACG8V3_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0300357.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="ACG8V3_001823"
     CDS             14643..14966
                     /locus_tag="ACG8V3_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300358.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="ACG8V3_001824"
     CDS             14959..15225
                     /locus_tag="ACG8V3_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300359.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DAZKMB010000067        15073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000067 DAZKMB010000000
VERSION     DAZKMB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.067"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACG8V3_001825"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACG8V3_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACG8V3_001826"
     CDS             706..1734
                     /locus_tag="ACG8V3_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0300360.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACG8V3_001827"
     CDS             1731..2489
                     /locus_tag="ACG8V3_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0300361.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACG8V3_001828"
     CDS             complement(2575..3315)
                     /locus_tag="ACG8V3_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0300362.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACG8V3_001829"
     CDS             3376..4629
                     /locus_tag="ACG8V3_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0300363.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACG8V3_001830"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACG8V3_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACG8V3_001831"
     CDS             5300..5626
                     /locus_tag="ACG8V3_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0300364.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACG8V3_001832"
     CDS             5694..6407
                     /locus_tag="ACG8V3_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0300365.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACG8V3_001833"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACG8V3_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACG8V3_001834"
     CDS             7690..9555
                     /locus_tag="ACG8V3_001834"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0300366.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACG8V3_001835"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACG8V3_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACG8V3_001836"
     CDS             11120..11821
                     /locus_tag="ACG8V3_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0300367.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACG8V3_001837"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACG8V3_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12857..13066)
                     /locus_tag="ACG8V3_001838"
     CDS             complement(12857..13066)
                     /locus_tag="ACG8V3_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFQ0300368.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13307..13882
                     /locus_tag="ACG8V3_001839"
     CDS             13307..13882
                     /locus_tag="ACG8V3_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0300369.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13882..14322
                     /locus_tag="ACG8V3_001840"
     CDS             13882..14322
                     /locus_tag="ACG8V3_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0300370.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14338..15039
                     /locus_tag="ACG8V3_001841"
     CDS             14338..15039
                     /locus_tag="ACG8V3_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0300371.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5084 a         2501 c         3179 g         4309 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    13201 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    13261 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    13321 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    13381 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    13441 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    13501 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    13561 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    13621 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    13681 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    13741 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    13801 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    13861 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    13921 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    13981 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    14041 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    14101 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    14161 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    14221 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    14281 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    14341 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    14401 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    14461 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    14521 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    14581 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    14641 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    14701 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    14761 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    14821 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    14881 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    14941 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    15001 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    15061 gaaagaaaaa tta
//
LOCUS       DAZKMB010000068        14895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000068 DAZKMB010000000
VERSION     DAZKMB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.068"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..253)
                     /locus_tag="ACG8V3_001842"
     CDS             complement(23..253)
                     /locus_tag="ACG8V3_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300372.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(689..1255)
                     /locus_tag="ACG8V3_001843"
     CDS             complement(689..1255)
                     /locus_tag="ACG8V3_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0300373.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1255..2211)
                     /locus_tag="ACG8V3_001844"
     CDS             complement(1255..2211)
                     /locus_tag="ACG8V3_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0300374.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2211..2867)
                     /locus_tag="ACG8V3_001845"
     CDS             complement(2211..2867)
                     /locus_tag="ACG8V3_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0300375.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3237..5255
                     /locus_tag="ACG8V3_001846"
     CDS             3237..5255
                     /locus_tag="ACG8V3_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0300376.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5325..5531
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5682..8096
                     /gene="leuS"
                     /locus_tag="ACG8V3_001847"
     CDS             5682..8096
                     /gene="leuS"
                     /locus_tag="ACG8V3_001847"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0300377.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8444..9997
                     /locus_tag="ACG8V3_001848"
     CDS             8444..9997
                     /locus_tag="ACG8V3_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0300378.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10378..12162)
                     /locus_tag="ACG8V3_001849"
     CDS             complement(10378..12162)
                     /locus_tag="ACG8V3_001849"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300379.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12177..13898)
                     /locus_tag="ACG8V3_001850"
     CDS             complement(12177..13898)
                     /locus_tag="ACG8V3_001850"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300380.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13998..14759
                     /locus_tag="ACG8V3_001851"
     CDS             13998..14759
                     /locus_tag="ACG8V3_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0300381.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4829 a         2755 c         2934 g         4377 t
ORIGIN      
        1 gtctcattaa aaagcataac cattactccg atctattttt aaacttagtc tctacctcta
       61 atatatatcg agtttttatt ttgccttcag agaatactgt ttcttttttt gcagttacag
      121 gttgtttatt ttcggaaaaa gctaatatag ctaaaattga aacatccatc tggaatttat
      181 cttttttact gctttgatca taaaagtctg catattcatc actgatattt tttctaataa
      241 attcttccat cataaaaatc acctcaaaac gattatacga acttacgttc ttgttgtaaa
      301 gcgatatttg aggggcaaaa aaggggcata atttgactga tattaagtta gctattgata
      361 tatgaatgac atacaaaaat ataaaatctt ttaaaaatgc ttatatatca atgtttttgc
      421 cattctttta tatatctgtt atatgtcaaa aaaatgtgtt caatttcaat ttgtatttga
      481 ataaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca atcaaaaaca
      541 gcttctcatg tttttgattt ggttggactt attaccgcag atataagctt ttgagcaccg
      601 tgaataaaga ggttatgaaa tcaacgctct aacctgagta ttagagaaaa aaacgcaaaa
      661 catatcattt atgttttgcg ttttttattt atcttttttt ctcaatacaa tataaaatag
      721 gcggctggtt cttttggttg ataaaccgat aattcagcac attatatgtt tcctgtggca
      781 gtttggaaca aaaagaatca accatatcca gttctttttc tccaccttca tgaccatagt
      841 agacgacaag gatcaatcgt ccgcgaggca caagtcgttt caatatttct tccatagctg
      901 tgcgtgtagt ctctggcata gtgatgattg ctttatcact ttttgggaga taccctagat
      961 tgaaaatccc tgctttgaga ttttgttgtt cagctatcac ttctcctaac gtttcgtggc
     1021 cttgtagaaa caatgagaca cgttcttcta actgacgctg ttctagcctt tcacgagtag
     1081 catcgattgc ttgttgctgg atatcaaaac tatagacatg accagttttt ccgatgtgtt
     1141 ctgctagaaa aaccgtatcg tggccattgc ccatcgttgc atctacgacg tgatcccctg
     1201 gttgcaagat ttcttgcagt aatgtatggc tgaaatgcaa agcagtttgc aacatcagac
     1261 gctcaccttt ttccgtacat catattttcc ttggaagcta tctctgcgaa gaagttcttc
     1321 atcaattgca ttcaagactt cccatttttt caagctccac attggtccaa tcaaggaatc
     1381 ccacggcgca tcccctgtca aacgatggat gatgatctct tttggtatca tttccagctg
     1441 gtcgcaaatg acattgacat agtcaggacg gctcattaat tgaaggcgac cttcatgata
     1501 gtcccgcaac attctcgttt tgcgcatcag atgcatcaaa tggagcttga ttccttggat
     1561 atctgaatct aaaatggtgc gtctgacatt ttctttcatc atttctaaac tttcgcctgg
     1621 taatccgttg atcaaatgcg tacaaacacg gatattatgt ttgcgtaatt tagccacgcc
     1681 atctaaataa gtttgataat catgtgctcg gttgatcagt tttgaagttt cttcaaaagt
     1741 tgtttgcaat ccaagttcta cccataaata catccgctcg ttcagttcag caaggtaatc
     1801 aactacttca tctggcagac aatccggtcg tgtaccgact gataatccta cgacacctgg
     1861 taaattgact acttgctcaa atcgttcttt gattatttca actggcgcat gggtgtttgt
     1921 aaaattttgg aaataaacga tgtattgttg gacatgaggc catttttgat gcatcatgtc
     1981 gatttctttc tggaattgga ttggaagagg gtcttcaggt gcaacgatca tgtctcccga
     2041 accagataca ctgcaaaaag tacaaccgcc atgggcaaca gtcccatctc gattcggaca
     2101 gtcaaatcct ccatcgatcg gcaccttgaa aattttcccg ccaaactcat ttcgtaaagc
     2161 gtaattccaa gaatgatatc ttttatttcc ttctgaataa ggaaaagtca ttaaaagtcc
     2221 ttctttctgt aagagttatc tatttgtttt cttttttctc tatacactgg ataagtcagg
     2281 cacagccaag aaagactgag taagtaaccg ccgatgatat cagtaggaaa atgaacgccc
     2341 agataaatgc ggcttatccc gacacttact ataataaaca tcatcagcaa ttgaagcaat
     2401 gtgcgtaagg ttcttttttg aacaaaagct ggaagcagaa atagtaaaga accgtaaaga
     2461 atcatactgc ctgctgcatg tccactaggg aaactgtaac ttgtttcaac tactaagtgc
     2521 gccaatgaag gacgctctcg catgaaaaat agtttgatca ccgggttgat gattccagca
     2581 attacaataa atccagaaac gaaccaaaat gtttctgccc tttttttctt ataccacatc
     2641 cataaagccg cacaagcaaa taaaatgatg acggtaagag gattaccgaa tttagtcata
     2701 aagagttggt agctgttcag atcaggataa gaagctctga caatgcctgt aaaccactga
     2761 tcgaaaccag aaagccacga cagatggaac tttacgatcc agcccaaaat gaaaaacagg
     2821 acaagaaaga agctaccagc cacctgaaat gatgttttat atttcataca attcctcctt
     2881 tgaagcaatg acttattata gcatttttgg tccagcgaaa aaaacggtaa agagataaaa
     2941 ccaatgtatg attcctcttt gaataaccaa tattcttata aaaaaaataa ctgtaatcgg
     3001 aaagacattt tttggtaatg caaatgaaat gttagggaaa taaaatcagt gtttttcgaa
     3061 atccttttaa acaagaacgt agacgtagga agaacttgaa aatgaacaga agttgttcat
     3121 tacggtaaat tacaatatga ttacaaaaaa caacaaagct cttaaataac ttttaataaa
     3181 tattaagaga agttataatt attaataata gataaattga agttggagga tagaagatgg
     3241 aaaacattgc aagaaaagaa cggagacgtt tagaacagac caaacgtttt cgaaaagcaa
     3301 aacgaggggc agcaatcgtg ggaactgcga tggtaggctg ttctgtcgct gccccattag
     3361 tacagccggt tcctgttcaa gcggatgaaa cacctgttca gttcggcgca agaatcaata
     3421 cagcggcatt tatcacagaa attgctactt atgctcaacc aatcgctcaa gccaacgatc
     3481 tatatgcatc agtgatgatc gcacaagcag ttgtagaaag cggttggggc agtagtgcgt
     3541 tatctcaagc accttattat aatctttttg ggataaaagg cagctatcaa ggacaaaccg
     3601 tctacatgga tacgcttgaa tttttaaata atcaatgggt aaataaaaag gagcctttcc
     3661 gacaatatcc ttcttttgct gaatctttca atgacaatgc ctatgtactt agaaatactt
     3721 catttggcaa cggttattac tatgccggta cttggaagag caacacgaaa tcctatacag
     3781 atgcgacagc ttgtttgaca gggcgttatg cgactgatcc tggttacgca ggcaaattaa
     3841 ataatatcat cgcaacttac ggattgaccc aatatgatac accagctagc ggaaacgcag
     3901 gcggtggagc gactacaggt ggcaataata ctggaagtgc aggtagtact ggcggttcaa
     3961 gcaattctgg taatacaaac tcaggttcag caacttcgac cacttatact gtccaatctg
     4021 gtgattctgt ttggggaatc tcaaataaat tcggtatcac aatggatcaa ttgatccaat
     4081 ggaacaacat ccaaaacaat ttcatttatc caggacaaaa actgacaatc aaaggtagcc
     4141 aggaaaatgg ctcttcgacg aataattccg gtaacaatac caattcttca ggaaatgctg
     4201 gtacatcaaa cggaggacaa acaactggtg cgaaatacac tgttcaatct ggtgattctg
     4261 tttggaagat ctcaaatgat cacggaatta cgatggctca attgattgaa tggaacaata
     4321 tcaagaacaa tttcgtttat ccaggacaac agctaatcgt atcaaatggc ggttcttcta
     4381 atgctggagc aaatactggc tcaaatactg ggaatacaag tggctctagt tcgtccagct
     4441 caagttcttc taatacagct tcaggaacga aatatacagt taaagccgga gactctgttt
     4501 ggagcgtctc aaataaatac ggaattacga tggctcagct aatccaatgg aacaatatcc
     4561 aaaataactt tatctaccca ggacaacaac tgactgtttc aaacagtgga tcaacaaaca
     4621 gcacgtcagg taatacaggt tcgtcaacca atacaaataa cagcagttca gttaatactg
     4681 gcagcagttc gtctcaaggc acttataccg tgaaagcagg tgaatctgtt tggagcgtgg
     4741 caaataaaaa cggcatcacg atggatcagt taatcgaatg gaataatatc aaaaacaact
     4801 tcatttatcc aggacagcaa ttgatcgtta aaggtggaac ttcggcaaat acaaatacag
     4861 gttcaactac ttctgcagcg aaaccaaata caccgaatac gtctgctacg acaagtacat
     4921 catccactgg taatacgatg tataccgtga aagcgggcga gtcagtttgg agcgtagcga
     4981 acaaacatca tatcacgatg gatcagttaa tcgaatggaa taatatcaaa aataatttca
     5041 tctatccagg acagcaagtg attgtgaaga aaggttcatc tcaatcaaca aaccaacaat
     5101 cagttactgg ttcgaaaacc tatactgtga aatctggaga atctgtttgg agtgttgctg
     5161 acagtcatgg aattacaatg gctcaactaa tcgaatggaa caatattaag aacaacttta
     5221 tttatccagg tcaaacgttg attgtagcta aataaaatag ctgttcgaag aaataacgat
     5281 tgaattccac aaaaatattt gttactataa ggctacttaa atatgacaac acttgaaaag
     5341 gaagtagtaa aaagactgaa gtttcagaga gcgcatggct ggtgagaatg tgcacggatg
     5401 cttttgaact cgcctttgag ttgcccttgc tgaatacccg agtaagcaag cggcggtgac
     5461 ggccgttaca acgttcgagg ccttttcact aaggtaaaat taggtggtac cgcgtagatt
     5521 acgtcctata aaaagagcaa tctttttata ggattttttt atttcttttg tgaagtttga
     5581 gaaaacaaag caaaaggaaa taaaaataaa cagattctgc cggttttttc gttatattta
     5641 taatagaaac aagtgttttc aatagaacag gaggacagca aatgagttac aatcataaag
     5701 aaattgagaa aaaatggcaa aaatactggg caaaaacgaa tactttcaac acccatgatg
     5761 atcctgaaaa gccaaaattt tacgcactgg atatgtttcc ctatccatcc ggtcaagggc
     5821 tacatgtagg gcatcctgaa ggttatacgg caacggatat cctttcacgt gtgaaaagaa
     5881 gtcaaggata caacgtgctg catccaatgg ggtgggatgc attcggtctg cctgctgaac
     5941 aatatgcgct agatacagga aacgatccag ctgagtttac ccaaaaaaat atcgaaacat
     6001 tccgacgcca aatcaattct ttaggattta gttatgactg gaatcgcgaa gtgaatacaa
     6061 ctgatccgga gtattataaa tggacacaat ggatctttac gaagctttat gaaaaaggtc
     6121 tagcttatga agcagaagta gcggtaaact gggtacctga attaggaaca gttatttcaa
     6181 atgaagaagt gatcgatggg aaaagtgaac gtggtggcta tgatgtagtc agaaaaccaa
     6241 tgcgccaatg gatgctgaaa atcactgctt atgctgatcg cttattggat gatctagaat
     6301 tagttgattg gccagaaagc attaaagaaa tgcaaagaaa ttggatcggt cgttcagtag
     6361 gagcaaatat tgaatttaaa gtggctggta cggataaatc ctataccgta tttacgacac
     6421 gaccagatac tctattcggt gcaacttact cggttcttgc acctgaacta gatcttgtta
     6481 gagaaattac gacccctgaa caaaaagctg ctgtagaagc ttatatcgaa gagacagcta
     6541 aaaaatcaga tctaaagaga acggatcttg caaaagaaaa aacaggagta tttactggtg
     6601 cttatgcaat caatccagtt aatggcaagg aaatcccaat ctggatcgcc gattatgtat
     6661 tatcttcata cggcacagga gccatcatgg ctgttccagc acatgatgaa cgtgactttg
     6721 aattcgctca aactttcgga ctagaaattc ttccagttat cgaaggtgga gatgttgaaa
     6781 aagctgctta cacagaagat ggtacgcaca tcaactctga atttctagac ggcatgaaca
     6841 aacaagaagc aatcgataaa atgaatgaat ggctagaaga aaatggcgta gggaaaaaag
     6901 aagtcagcta tcgcttacgt gactggctat tctcccgtca acgttactgg ggtgaaccaa
     6961 tcccaatcat ccattgggag gacggaacag tcacagctgt cccagaagaa gagctaccat
     7021 tacggttgcc taagacaaaa aacatcaagc caagcggtac tggagaatct ccattagcaa
     7081 atatcgagga gtgggtcaat gtcgttgatc ctgtaacagg taaaaaaggc cgtcgcgaaa
     7141 cgaatacaat gccacaatgg gcaggaagct catggtatta cctgcgttat atcgaccctc
     7201 ataacaaaaa agaactagca gattatgaaa agttgaagcg ttggctacca gttgacatct
     7261 acattggtgg agcagaacat gcggtacttc atttactata tgcacgattc tggcataaat
     7321 tcttatacga tatcggtgtt gttcctacaa aagaaccatt ccaaaaacta tacaatcaag
     7381 gaatgatctt aggcgaaaac aacgaaaaaa tgtcaaaatc tcgtggtaat gtagtcaatc
     7441 ctgatgatgt ggtcgaaact tacggtgcag atacactgcg catgtacgaa atgttcatgg
     7501 ggccattaga tgcttcgatt gcttggagcg aaaatggctt agaaggaagc cgtaaattct
     7561 tggatcgtgt atggcgtttg atcgttgatg agaacaataa aatgcgtgac cgtatcacta
     7621 cattgaatga tggcaagctt gataaagtgt atcatcaaac agtgaaaaaa gtgacagaag
     7681 actatgaaaa tcttcatttc aatacagcaa tctcccaatt gatggtcttt atcaatgaag
     7741 cctataaagt ggacgctctg ccttatgaat atatcgaagg atttgttcaa ctactcgcac
     7801 cgattgctcc tcacatgggt gaagaactat gggcaatcct tggaaatgat ggtggtattt
     7861 cttacgcacc atggccaaca tacgatgaag cagcacttgt tgaagacgaa gtagaagttg
     7921 ttttccaagt aaacggtaaa gttcgtgcga aaagcaatgt tccccgagac ttaggaaaag
     7981 atgagctaga aaaagcagca ttagccaatg aaatagtgca agaatatatt gaaggcaaaa
     8041 cagtcagaaa agttattgcg gtaccaaaca agttagtcaa tatcgttgct aattaattgg
     8101 ctctttgtca ataaggactg atgagttgtg caaaatcaaa tctgggccag aatgaacttc
     8161 attctgtctc agattttttg ttatttcaaa ttgattaatt gattgatcaa aaaggttttg
     8221 attttcatgt tctcaaacac gttcaatgtc tttttcttct gaatggtctt gtaagagatc
     8281 tctgtaatca tcaaattttt gtctattaat cttagtattt tttgatgtat aaactacctg
     8341 aaattgagag tacataccaa attcataaat tttttattag tgggactagt gattgtatgg
     8401 attttgacgc ataatgaatg aagtatgaga aaaggagaga caaatggatc aagaactatt
     8461 tattatcgaa gtattatcag ctaaacaaaa aattatcgag aaagcgaaaa aaagagcgaa
     8521 aagaataatg ttcaagcttt taagcttagt atttttctct ggtttgcttt ttttcggagc
     8581 tgtatcgttc ctatatcgcc atatgagtgc agtagtccgt tcagctgaag ggatgcaaaa
     8641 attaaataat ttgtatcact tttcgtgggg agttggcgga ataacaattg ttgtgcttat
     8701 tgtttttctt ctacttattt ggctccgtta ccatcaattg tcgcgtcatt tcgatcaatg
     8761 gcttagtcaa agagcacaac agtttttaca taataaacca acttctcaag atgatgacta
     8821 tctttatttt gagaagactt cgacgagtca agcttttcgg atacaaaaaa gtgattgcca
     8881 gttattgctg agatcacttg atgatttacc tatctactta ggcgaaagca aatcaaaatt
     8941 cggaatgaac acattccata tttttttact agaaaacgaa cccagatggg aaaaaagcca
     9001 gtcttcgcct ggtggatacc tgacaactaa gatgagtaca ttagtcactg gttttttcct
     9061 cattctttta gctggaacaa tctattatgg gagatacact cagccaaaaa tgtcttctca
     9121 tataaatgct gcttctcaca tgacaatgaa ctcaactaac gatacgcata tacaaaagac
     9181 aaatgaaggg ttgttagctg atcctaagca aaaggtaaat ttgacaacac aaaagataaa
     9241 tgatctcaaa cttgatacag agacgcactc tctctatatg acaacagata gtggtgctaa
     9301 ttgggtattt gtgccgattg attcgcaatg gttgcgtttt ggcgaataca ccctgacaac
     9361 ggggctgatt cctgaaggtt attggatgga caacacatat gatatttccg ccgatttctc
     9421 atggtttatc tattcttctg gggatgatgt acgtctgctg acttcaagag ataatggtaa
     9481 aacatggcaa acgtctatta tcacccaatc agttcagccg atacgttatc gaaaagcggt
     9541 attttatggc aacgatggtc tatttatctg tacatcagcc agtgggatgt ctgcagaaac
     9601 tttgtacatt tacactacta tggatggcgg aatcacttgg ataaaaagtg gaagtacaat
     9661 gatcaatcag ccagtacaaa atgtcagctt cgttacaact tctttaggtt ttgtttcgac
     9721 acgtgaaagt ttgttttata caaacaacgg aggacgctca ttcaaagaat cagttctgtc
     9781 gattccggaa aactatcaga caggggggat agatcttttt caatcaccaa atgaagttac
     9841 tcaaataagc gcgaatacgt tagagacaaa attttatttg ttgaaaaaag gaccaatcga
     9901 tgcagggaaa atgtttgcct gcctcttccg ttcaactgat aacggggaaa catggcaatt
     9961 cgttcagcaa ctatcgcaag tagaacaagg agattaaaga agagagattg tgagcctgac
    10021 gttcagcacc gagtcacaag aaacaaattc aaaaaacagc ttctcatatt tgttgaatag
    10081 tgtaactcgt gattcggagt tgacaagagc aacaagaagc gattttgaag tatagcttct
    10141 cgtattttac aagatcgtgt cttattgccg ataggttgtc tcttgaacac cgtttattcg
    10201 taaaaaggga ttgtgacaag tcttttgtca ctacttaccg aacgaaactg aaggagctat
    10261 ccaaaaagta tcattagtat tattctttgt gtttctagtg gcaccactag tacatttaaa
    10321 aaagctatcg atcatggact aatcatacat gatcgatagc tttttagata atgtttgtta
    10381 cgctatctga aattgactat tataaagact ggcataaagc gttggtgatt ggagtagttc
    10441 agaatgtgtt cctttttcga cgatatcgcc attcttcatt acaaggatca tgtctgcatt
    10501 ttcaatcgtt gacaaacggt gtgcaatcac aaaacttgtc ctatcttttg tcacagcttc
    10561 cattgcttgc tgaatcaatg cttctgttcg tgtatctacg ctagaagttg cttcatctaa
    10621 aataacgaca ggaggattcg ctagaatgac tcgagcaatt gtcaataatt gttgctgtcc
    10681 ttgagataac gtgccgtttt cgctagaaat gatcgtatca tatccgtctg gcagtgttcg
    10741 aataaagtga tcacattgtg cgatttcagc cgcttcgata atttgttctc ttgtggcatc
    10801 cattttcccg taggcgatat tatcagctac cgttccttca aacagccagg tattttgtaa
    10861 aaccatccca aaagaacgac gtagttctcc acgtgaaagc ttggtaatat caagaccgtc
    10921 gaattcgatc tttcctttat tcagttcata aaaacgcatc agcagattga ctaaagtcgt
    10981 ttttcctgca ccagttggac caacgatcgc taccgtttgt tttggcttta ctgaaaaatc
    11041 gacttgattc atcaatattt tatctgatgt gtaaccaaac tgtacatttt tgaaagctat
    11101 ttttcctttc ggttgttcta aaagtcttgc atctggaagg tctggaattt catctgtttc
    11161 atctaatatc tcaaatatcc tatcgattgc tgccattgcg gattggatcg agttgatcac
    11221 ataagaagcg gtagaaattg gttcagaaat ctgattgatg tactgaagat aagcttgtaa
    11281 gaagccgatc gtcacaccac ctgatagaac aagcatagca ccgaataccg cacttatgat
    11341 gaaggcgagt tgattaatga aacgaatggc cggatagata gcacagttca taaattgagc
    11401 acgtttaaaa gcgttgtatt gctttttatt tacttcgtgt atagcagctt ctgcatgagc
    11461 ttgctgattg aatgttttga ccactagatt accagctaag atttcttcca ttcgattatt
    11521 taaaacaccc agttcatact gattctgttc agaaatctgt ttgtttttgt tcgcaatctt
    11581 agttgtaaca attgaactgc caacgatcag caataagacg agcaaagtaa gcttgctttc
    11641 gatataaaac agcatcagac cagcagcagc tattgttacg acagaagtaa aaaattgatt
    11701 gatacctgtc aataacactt gagaaagttg attgagaccg gtagttgtcc gactaatgat
    11761 gtcacctact tgatgattgt caaaaaacga catcggtaat gttttgaatt tttttgtgat
    11821 ttcttttcgc accctcaatg tgatcttttc gcttaacgct gccattgtcc gttcctgaat
    11881 aaatgaagtg atgctgctta agactgaaca gccgatcagc aataagacag ggaacaacaa
    11941 tgcttgtttg atatcactga aagaaagctg tccaatacct gtttttctta tttctgataa
    12001 gagatcatcg atcccgattc ccatgatcaa gggcatggct acgactaata ggttcccgaa
    12061 taaactgcat agaaacaatc cgtaaaaata tttatgttca ggtttgatca ttttccaaaa
    12121 acgtatcaaa gcacgcgtgt tcaacgtttt tttcttttcc atcgtatccc ttctttctat
    12181 attccttgtg attttgcaaa ctcctgataa gtttggcaat tagacagtag ttcttcatga
    12241 gtaccttgtc caacgatttt tccttcatct aaaacaatga tgttatctgc ttgttggatt
    12301 gtactgaggc gttgagcaac aatcacgaga gttttctccg acatgtgttt atgaagggct
    12361 gctcttaatg ctgcatctgt tttatagtct aaagcggaga aactatcatc gaatacatag
    12421 atctctgctg gtttgatcaa cgctcgagca atacatatcc gctgtttctg gccccctgaa
    12481 taattagaac cgccttgagc aacaaagctg tccaatccgt caggaagggt gtccactagt
    12541 tcttctaatt gagcgatctt gatcgcttta gacaactctt ctgttgaggc atattcattt
    12601 cccatctgca agttgcttaa aattgtcccg ctgaataaga aagccttctg agggacataa
    12661 ctgatgtggg agcggatctg ttcttgagtc atttgagtaa gaggaatacc accaagggtt
    12721 acttttccta tcgtagggtc actaagtctc aagagaagtt tcgccaaact actttttcca
    12781 gatccggtcc cgccaacgat cgctgttgtt tttcctttag gtattgtgaa atcaatatct
    12841 tctagaactg gatcagagat ttcagtatat ttaaatgtga catgttcaaa agcagctgca
    12901 tttgtctgat caaaggttgg ttcagctact gttttgatcg tctcttcctc aatttcgatc
    12961 tctgcttgca gtatttcttc tatccgtcgt aatgaggcaa acgatttagg gataatgacc
    13021 aataccatag ctgcgatcat caagtaagca agtgtcagca tagagtattc gactacagca
    13081 gtcacgacac cgatttgtaa tgctccttct gctacaagat ttccgccaaa ccacaaaata
    13141 gaagcataaa cgattcccat gagcaaaaaa gcaattggag tgacccaagc aaatattcgg
    13201 ttcactttga tcatttgatt agcatagtca gcaaagctct tattcgttcg cttttcttca
    13261 tatgattgat tatcaaaggc acggatcata ttaatcccag taaagaattc tctcaatgtg
    13321 atggtgatcc gatccatttt tggctggata ctcaaagata aaggtgcccc tcgtttcatc
    13381 agtcgataaa caatccatac ataaaaaaca acagagatca atgtaaccca tgttagtaat
    13441 agagaataac gaaacatcat gagaatggtg aaaacgcaaa taaccggtgc tggtaagatc
    13501 atttggcaaa ataaaacaat catttgctgg acattatcga catcattcgt cattcttgtc
    13561 aaaagagatc cagaactaaa acgactgata tccttcattg acagctgttg gaatttacgg
    13621 aaatatttct cccgcgtttg gaatccaaac ctagcggaag ccaatgctga aaagtaacta
    13681 gaggcaactg cagcgattgt tcctagaaac gctacaagca gcatttcaac tccaattttt
    13741 tttataactg aggcttcgcc acttgctata cctgtgtcga tcagctttgc tactaatttt
    13801 ggtacaccta atgtaccggc gatttctaaa cagatcatca caaaggtaag gagagcgagt
    13861 cctttgtttt tctgtaagaa tgtttttaag atagacatat tattcctcct tttttcctga
    13921 tacttttcga ttctatacct tcaagtaact tgaaggtcaa tagaaaaaag aataaactga
    13981 aaaagaggtg agaaaaaatg aaaaaaatgt atacgattgg ccaagtatcg gaactatttg
    14041 atttgccaaa atcaacgatc cgctattggg atgaacaagg attaatccgt tcaagtagac
    14101 aagaagagaa tgattatcga ttgtttgata tcgatgatat ttttatgctt tatgatatcg
    14161 ttttttatag aaaattagat ataccgatca aacagatgaa gaatttatat ggaaaaacat
    14221 tgacggagtt atatgagaca cttgatgaaa cagaaagaag aatccataaa gaactggtgg
    14281 tgatgaaaca aaaacaaaaa gagataaggg agcaaaaaaa acagttgaaa ttgatgatcg
    14341 atacaaacga ggaagaattt cctgtagaag agatcccttt tgactgtatg atctcaacgg
    14401 agtttgaaga tattgtggaa atcaaaaaat ttctacctaa ctattcaagc tttgggatga
    14461 tgagtatgcc agcttccagt tcacagacga tgtatggatt ttttatcgac cctagtgaag
    14521 tacatttatt tactcaagac gtgatttggg agaaaaaaga tacggctgtt tatcgtcgat
    14581 tcttattgaa atccgagatg aaccatgcgg aacgtaataa tattttagaa atcagagaaa
    14641 gaatgtatga aaaaggatgg aaaacaggag aagtgatcgg ccagtatcta ttgacgaata
    14701 cggatgaaaa caatatcaga actgaatatt atcatgcatg gatagaaatg aaaaaatagg
    14761 aaatatgtgt ttaagataac tcgaaaatga tggttgtttt agttataaag aaaaattttc
    14821 tggttcgatt acgggtttaa tgtgggggat tattagtcgt ggatagatag aattttagtt
    14881 ttttagagga aatat
//
LOCUS       DAZKMB010000069        14625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000069 DAZKMB010000000
VERSION     DAZKMB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.069"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG8V3_001852"
     CDS             4..816
                     /locus_tag="ACG8V3_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0300382.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG8V3_001853"
     CDS             813..1613
                     /locus_tag="ACG8V3_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0300383.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8V3_001854"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8V3_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0300384.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG8V3_001855"
     CDS             3110..4819
                     /locus_tag="ACG8V3_001855"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0300385.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ACG8V3_001856"
     CDS             5031..9389
                     /locus_tag="ACG8V3_001856"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0300386.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8V3_001857"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8V3_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0300387.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8V3_001858"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8V3_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0300388.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ACG8V3_001859"
     CDS             11227..11520
                     /locus_tag="ACG8V3_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0300389.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ACG8V3_001860"
     CDS             11517..11828
                     /locus_tag="ACG8V3_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0300390.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ACG8V3_001861"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ACG8V3_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0300391.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLQEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8V3_001862"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8V3_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0300392.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4982 a         2599 c         3255 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgcaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DAZKMB010000070        14129 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000070 DAZKMB010000000
VERSION     DAZKMB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.070"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..819)
                     /locus_tag="ACG8V3_001863"
     CDS             complement(<1..819)
                     /locus_tag="ACG8V3_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0300393.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKN"
     gene            complement(792..1472)
                     /locus_tag="ACG8V3_001864"
     CDS             complement(792..1472)
                     /locus_tag="ACG8V3_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300394.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1495..2433)
                     /locus_tag="ACG8V3_001865"
     CDS             complement(1495..2433)
                     /locus_tag="ACG8V3_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0300395.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2678..3529)
                     /gene="ispE"
                     /locus_tag="ACG8V3_001866"
     CDS             complement(2678..3529)
                     /gene="ispE"
                     /locus_tag="ACG8V3_001866"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0300396.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3683..6256)
                     /gene="mprF"
                     /locus_tag="ACG8V3_001867"
     CDS             complement(3683..6256)
                     /gene="mprF"
                     /locus_tag="ACG8V3_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0300397.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6397..7203
                     /gene="proC"
                     /locus_tag="ACG8V3_001868"
     CDS             6397..7203
                     /gene="proC"
                     /locus_tag="ACG8V3_001868"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0300398.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7282..8838)
                     /gene="rny"
                     /locus_tag="ACG8V3_001869"
     CDS             complement(7282..8838)
                     /gene="rny"
                     /locus_tag="ACG8V3_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0300399.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9249..10298)
                     /gene="recA"
                     /locus_tag="ACG8V3_001870"
     CDS             complement(9249..10298)
                     /gene="recA"
                     /locus_tag="ACG8V3_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0300400.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10407..11648)
                     /locus_tag="ACG8V3_001871"
     CDS             complement(10407..11648)
                     /locus_tag="ACG8V3_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0300401.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11888..12466)
                     /gene="pgsA"
                     /locus_tag="ACG8V3_001872"
     CDS             complement(11888..12466)
                     /gene="pgsA"
                     /locus_tag="ACG8V3_001872"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0300402.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12675..13598)
                     /locus_tag="ACG8V3_001873"
     CDS             complement(12675..13598)
                     /locus_tag="ACG8V3_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0300403.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         4044 a         3150 c         2469 g         4466 t
ORIGIN      
        1 attttttctt ctgaaggcaa ccaatattcg tttgatgata tttacaatca ggaacaaagc
       61 aataaagact aatgtgattg ttgctcctgg aggtgtatct agataaaacg agctagttag
      121 tcctgacagc atccctagaa atcctacaca aacactgata gcaatcacga cattgaagct
      181 tttgcctaat cgcataccga ttgctgcggg aagaaccatg attgcggata tcaaaagtgc
      241 accggcaatc gggatcatca cagcaatagc cacaccagta atcacattga acatcatcga
      301 catcaaatga acgggtagcc catccacgtg ggcggtatct tcatcaaaag tcagaacata
      361 catcggccgt ttgaataaaa agaacaatat accgatggca acgaataaga ttcctaaaaa
      421 gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac agataggatt ggatacttgt
      481 tgctgaattc cctccgctca aattcatcaa tactaacgct agcgccaaac caccagccat
      541 taaaatggca atggagattt ctgaataggt tcggtagatc gttcgcagat actccaatat
      601 cacagctgct aatacaacga ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa
      661 tcctaacgct acccctgcta aagagacatg agaaagcgta tcagccatca gcgattgtct
      721 tctgatcact aaaaacacac ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag
      781 aaacgctctt ctcatgaatt catatgaaag caacgccatt gagaatcctc ctttctcacc
      841 agtcggatct gtctgtccgc atactgtttg atatcttcat gatcatgggt gatcattaga
      901 actgatttcc catggacatg cgcactatgt cggagaagac gataaaattc atttctggat
      961 tcttcatcca tacctgttgt tggttcatca agaacaaata agtcagggtc agtagcaaaa
     1021 atacgcgcta aactgattcg ctgcttttgt ccgccagaca attcacctat tcgccgattt
     1081 cgcatatccc acatccctac agcttctaaa gcacgctgta catgttgatg atctttttca
     1141 gagagacgct tgaaccagcg gcctcttgga tatcttcctg aacggactaa ttcgatcacc
     1201 gtactaggaa aacctgcatt gaaagatgca acttgttgag gaatataacc gatacttaat
     1261 ttttttcctt cttgattttt ctcagcaatc tttatcattc cttttgaagg tttcagtaat
     1321 cctaacgatg ctttcaccaa ggttgacttg gctgctccat tttctccagt taaaatgaca
     1381 aattcacccg catctacatg ataagagaca tcttctagta caggttcatc atcatagtaa
     1441 aaggtcaaat cagatatttc gatataacgc accttacaac ctccattcta aaacttaatt
     1501 gatactttct tttaaagctt tcagattttt ttccatgaca gaaatataat tttcgcctgc
     1561 tttgatttgt tcattagtca agctttctaa aggattcaac acttctagct tcactcctgt
     1621 ttcattcgac aatgtctcag ctaccttcga agaggcattt tcttcaaaat aaatcacttt
     1681 gatgttgttt ttcttcacaa attcttttag ctcagctaag cgacttggtg tgggttcctg
     1741 atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg tattgattcg ctaaataagc
     1801 aaaagcagca tgctgagtga caaatgtgcg gtttgttgca tcttttagct catcggagta
     1861 tttttgatcc agtgcttcaa gctttttgat ataagcatcg gcattcttcg taaaagtttc
     1921 ctttttatct ggatattttt cgcctaattc ttcagcaatc gttctgactt cttttatagc
     1981 taataccgga tcgagccaaa catgtggatc gtattcatgt gaatgaccat gcccttcttc
     2041 tgattcttca tgttcgtctg cttcttgcga ttctaaacgg tcgattcctt tagctgcttc
     2101 gatcatcaag gtctgtttgc tatccactga atcctttaac gaatccacaa aaaattccat
     2161 gtctgaacta ttatacacga aaacatctgc atcggaaatt tctgctcttt cttttgcact
     2221 tggttcgaaa tcatgcggct ccgttccagc agggatcaaa agtttcacgt ttccttcatc
     2281 ccctacgatc tcttttgtaa aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc
     2341 ctcttttttg tctgcttcct tgttcgtatt tccacatgct gcaaacagta aagcacctat
     2401 cattaccgta gctgtcaaaa gatatttttt catttatatg ctcctttcaa tgaacaaatc
     2461 ggaattttac gatttattaa cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag
     2521 aaatccatca atttctgatt taagcatgta tattcttgat taaatcgtaa aatggatgag
     2581 ttgctgctca tatctagcca tttcactaca ataaaaaact gaagaatacc attttaggat
     2641 tcctcagttt ttcatcacct attttacttc ttatatttta ttgcagggtt cgtactaaat
     2701 aaacttcctc acaaaaacct tttaacccat tatatactct ctgcgctctt gaataatgtt
     2761 gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa tgcagcatct gctccgtact
     2821 tgatcatacg ctctttgatt tgatggatga ctggatgccg ctttcccgtt atatcttcta
     2881 aactgtttcc catttttttg atcatctgct gataatcttg ctctcggatg gcttcagcaa
     2941 gtccgtggat gtcttgatgt tggatggctt gcgtatctac ttctctaaac actgtacgtg
     3001 tagagacact cattcttggt ttgaccaaca caacccagca ctgtggcata gaaggtaata
     3061 cttggatttc ttcccctttg ccaccgacaa atgccgtact tccgtagaca cagtaaggaa
     3121 catctgtacc gacttccaaa ccgatttctg ccagttcttc taacgaacag cccagattcc
     3181 ataattggtt gatccctctt agagcagctg cacagtctgt acttcctccg cctaatccag
     3241 ctgctactgg aatattcttc ttaacaaaga tcttgacacc ggttttgata ttgtatttat
     3301 tttttagcaa taaagcagct tgataaacat tgttccgttc atctaccggt aaaaatgctt
     3361 tatttgtttc tacaatgatt ttgtcttctg ttatttcttc aaaaaataga tgatctgcca
     3421 agtctacgct cgacatcacc atttccaact catgataccc atcttgccgt ctatataata
     3481 catccagtcc taaatttatc ttggctggcg ctttttcaat caattccatc ctctcaccca
     3541 ttcgaaaagt ctaagtcgca agtattctat catagggaaa aataaaagta tagcttatgt
     3601 ctgcctagtg gttgattttg tcatcgtttc aacagctttc acagaatttt ctcttcataa
     3661 tccaaacaag ttctagtgat tcttataatt ctgtgagcac ttcttctgcc aaactcggaa
     3721 catttccttt ttcttttcct atcagactga gtagctgcat ggtagcaaat ccgaaattac
     3781 ttcctttcct ataagcaaaa taacgtcctt cccaatgatc gacatatttt aatttctttt
     3841 cataagcaga ctggaaggaa tagaaaaaat taccataatt atatacgata tgcatgactt
     3901 tttcttgaaa gaaagaaaaa tctgtttctc caacattagc taatggagct aagcctagat
     3961 tggcttccga gtagcccatt ttctcgtata caataaacag attggctaac agatagtcag
     4021 ctaattcctc agattcttta ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg
     4081 tcatcggctg agcggtgatg aagccaacga ctttttgatc tttacggaca ataccgacac
     4141 cgctagataa tacatatgat ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac
     4201 cttgaagcca tttttgtgaa acttcattta cagcttgatg taattcttcc ggcagttgat
     4261 cgtaccaagt aaaatggtat ccttctttca tcaaacgttg atattcaagt tgctgactca
     4321 gccgagacaa gtcttctgtt gctagatcaa caatccctgc ttcacctact ttcgtgaagt
     4381 gaaaacccaa ttcatgaagc atcactacgt agctttctga tatcttataa aacgcaagct
     4441 gatagcctaa acaatctgct tgtttcataa actgattcgt tgctgcccgc atcattttag
     4501 gattgccaac tggatctcct aaaacaaagc atttgtttcc tttgatctga taaccaaaaa
     4561 cgacttgatc ttggtcgtct tcttggtaat aaaaataact gtaacctgac aagcgaagct
     4621 gatggcttgt agctgtgcct ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt
     4681 actcactgcc aagcttcgta gtatcttcag ctaaatattg ataaagcgcc actaaagcta
     4741 gcaatgagat gcccaaacca ataagccctg agaaccacac atcatctgaa ggaaacaaaa
     4801 taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg
     4861 cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa gattatagct ccccaagaat
     4921 aaacgaattt tgttcgataa aattcttttc tggataacca cacagctaac agaatcaaga
     4981 catataccaa tatcaactgc caagataacg tacgtgtgat tgtatttaaa ataccaaaag
     5041 atagtaaagc aatcgtaggc agaaatgcct ttttgacttt tactgaaagt gctctagcaa
     5101 ggccgagtaa taaaaagcca accaatagat tcactgtctg atctaaaaaa ttgaatgaga
     5161 acggcaataa tacttgaaat agccgactga ttgcagacag attagtgatt gttgatagaa
     5221 gtaccatcat gatccccgca aaatagacag cacttacgag cacgatatgc gcaacctttt
     5281 ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat tttgactcct gtatgtgtga
     5341 tgaacaatag aagtcccgtc ataaaaggta gaacataata aaacaaccga taatataata
     5401 gccaaactac tgctacatct tgccgaacag acaattgact cattcctaag atcatcaaaa
     5461 cgtcaaaagt ccccattcca cctgggacca tcgtcagcat tcctatcaat gtagcaatca
     5521 tgaacatagg atagatcgaa agcatcgaaa tatctattct catcaacttg ccgatgatca
     5581 aaaataccgc aagggctccc gtccattgtc caaaggatgc cagtagcaga ccaagcaagc
     5641 cttttggata aaactcgaag aacaatgtct ttctctttag ataagcaaaa aagaaaatga
     5701 ctggtgctaa aaaactaccg ccgaaaagcc agatccaata gcttcgaaaa atactttctg
     5761 actgaataaa aaagacatca ataaatgtaa gaaacgacca tacagacaaa ccagtcagca
     5821 taaatagtgc gacttttgat acagtggcta aaaccatttt tctatttgtc tgctttccat
     5881 aaaaactagc ccgcagactt gctcctacca caccaccaaa accggcgaga ttgttgatcg
     5941 tgtttgttac ccacgcagca agaagaaact cttttctttc catttttggt tttccttgtt
     6001 tttctaagac tctgatcgtc acccaatcat acaacaacat cggagtgaca cccagcagtc
     6061 cagccagaat catgagcagg atcgtcatgc ggctttgttg tcccatcctt tgccaaatat
     6121 cttgccaact catgccatta gcaatatttg cgacttgatt tgccacaaag actaacacgg
     6181 agccgaaaaa aatcagtttt aaaatcagac tatgttcctt tatccattgc agcatcaagt
     6241 atcgtttatt tttcacattt tcccattcct aacttgtata cttttgtgtc ataccccaat
     6301 gttatcttat ttattttaat ttttccactt tagacctgtt ctttgcttgc gatgatagaa
     6361 aaagaaagat atcctaatgc taaaggagag gataacatga aagtaggatt tatcggcaca
     6421 gggaatatgg cacaagctat tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa
     6481 gaggtgttcg tttccagtgg acattttgaa aatgcacaaa aatttgctgc tgaaactggc
     6541 gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat cagatatcgt tcttttagcc
     6601 gtgaaaccaa aaatcattga aaaagtattg gaagaactaa gagaatatag ccagaaagtc
     6661 ttattcattt ctattgcttc aggaaaatca ttaatagatt tagaaacagc gcttgaagat
     6721 cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt
     6781 tctgcgattt gcaaaaatga agctgccacg gaggaacagc ttcagcaagc tacgatgatt
     6841 tttgaagcaa tcggcagcgt ctatccttta gcagaatcag atttcagtac attcattggc
     6901 cttgctggca gctcacccgc attcgtttac atgctgatcg atgcgatggg acggactggt
     6961 gtactgaatg gtttgtccaa acagacagcc actgagattg ctgcaaaagc aattatgggt
     7021 tcttgtcaaa aattcctttc aagtgataaa aatccatggg aacttgtaga ccaagtttct
     7081 tctcctggcg gtacaactgt tgcaggtgta gttgccttag aagaagcagg attcatccca
     7141 gcaatcatca aaggaatcaa tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa
     7201 taatgatagc aatgtttttt cgtgccttac aaaaaaagaa gagctgtggc ttttgccgcg
     7261 gctcttcttt tgtatttttt attatttagc ataatcagtt gcacgagttt cccgaataac
     7321 gattactttg atgtgtccag gataatccag ttcttcttcg attcgtttac gaatatcacg
     7381 tactaaacga acagcgtcta aatccgagat ttcttctggc ttgaccatta cccgaacttc
     7441 gcgtccagct tgaactgcgt aacttgattc tacaccggcg aagctgttcg aaatattttc
     7501 cagacttctt aaacgacgga tgtagttttc taatgattca cttcgcgcac caggtcttgc
     7561 tgctgagagc gcatctgctg cagctacaag gacagagata acagaagtag cttccacatc
     7621 tccatgatga gaagcgatcg cgttgattac tactgggttt tctttgtact tggcagctaa
     7681 ttccgcaccg atctcaacgt gagatccttc aatctcatgg tcaagtgctt tcccaatatc
     7741 atggagcaac ccggcacgtt ttgctaattg gatatcttcg ccaagctctg ctgctaagat
     7801 accagctaat tttgcaacct cgaccgaatg cttcaagacg ttttgtccat aactagttct
     7861 gaatcgcaga cgtccgatga tcttgattag atctggatga agtgtatgag caccgacttc
     7921 aaacgctgcc tcttccccgt attcgcgaat acgttcatcc atttctttac gagatttctc
     7981 gaccatttct tcgatacgcg ctggatggat tcgtccatct tgtatcagtt tttccaaggt
     8041 catccgtgcg atttctcttc ggattggatc gaatccggaa agaacaactg cttccggtgt
     8101 atcatcgatg attaaatcga taccagtcag cgtctcaagc gttcgaatat tacgaccttc
     8161 tcgtccaatg atacgacctt tcatttcatc attcggaagg gtaacgactg aaacagtcgt
     8221 ttctgaaact tgatctgctg cgcagcgttg aatcgctaat gataataagt ttttcgcttt
     8281 gcgatctgct tcttctttgg cacgctgttc agattctttt accattagag ttaactcatg
     8341 attcagctct tcttcagtcg atttcatgat gacatctcgt gcgtcctctt ttgataaacc
     8401 agcaattttt tctagttctt tttgttgatc ttcaatcatt ttttcaacgt ctttttctcg
     8461 ctcttcaatt aattgctgtt ttgaactaag tgcttcttct ttactttcaa gtgagttctc
     8521 gcgtttttct aacgagtcat ctttacgatt cagcgtttgt tcgcgttgaa tcaaacgatt
     8581 ctcttgagat tttaattcta acttactttc tctcagctca ctttcaactt cagaacggta
     8641 tttctgattt tcttccttag cttctaacaa tgcttctttt tttagagttt ctgcttcttt
     8701 ttttgcacta tcgatgattc cagcagctga tgattgtgca ccagctattt ctttctcgtg
     8761 acgtgacttt gcgaccataa accctaaacc aagaccgaca attaaaccga tgatagcgag
     8821 gagaatgttg aataccataa aatccacctc catactatct ttttcatttt tatcatctat
     8881 acagcttcta tgataaatta ttctaaactc gtcaatatgc atcttcgcat atatgtgtac
     8941 aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt agtgtcaact ttaaataaac
     9001 aaagaaacgg ctttctactt attcattaaa cgatggtttt taaaataaat gacactagtt
     9061 tcatataggt aaacctttaa attgagaaac ttttctatta aattattctt tgtagatgaa
     9121 cagatatttt tattttatgt acccactcgt ataattaaat aactcacgat ttctttattc
     9181 aataaaaaaa cggcaggtca aggagagtac ctactcttca gaccagccgt ttttgtttat
     9241 cataattttt attcttctac taaaggcaat tcttcttgtc ctttttcatc ttctgggaca
     9301 gcgacttctt caccaatacc ataagctgct ctaactagag cagaaacttc tgccatcatt
     9361 tcaggatgat tagccatata gattttagcg ttttcccgtc cttgaccaat ccgatcttct
     9421 ttataagaat accaagcacc gcttttatca acaatgtctt tttctactgc catgtctaga
     9481 agttctcctt cttgtgagat ccctaaacca tacatgacat ctacttcagc gattttgaaa
     9541 ggaggtgcta ctttgttttt tacgactttg atctttgtac ggttaccaac aatatctgta
     9601 ccttgtttca attgttctgc acgacgtact tccaaacgga tcgttgcgta gaatttcaat
     9661 gcgcgtcctc caggagtgat ttctggattc gcaaacatca cgcctacttt ttcacggatt
     9721 tggttgatga aaatagcgat tgtttttgtt ttgttgatcg aaccagagag tttacgcaat
     9781 gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt cacccatttc gccgtcgatt
     9841 tctgctcgtg gaactaaagc agcaactgaa tcgactacta cgatatctac agccccactt
     9901 gatactaaag catcagcgat ctctagacct tgttctcctg tgtcaggctg tgaaagaagt
     9961 agttcatcga tattcacacc taatttttgt gcatattgcg gatctaacgc atgctcagca
    10021 tcaatgaaag cggccgttcc gccatttttt tgtacttctg caatagcgtg tagtgcaact
    10081 gttgttttac ctgaactttc aggtccatat acttcgatga tacgtccacg ggggtaaccg
    10141 ccgacaccta aagcgacatc taacgcaaga gagccacttg gaattgtaga gatttgttgg
    10201 tcgatttttt cgcccaattt cataatagag cctttaccat agctcttttc aatttttttc
    10261 agtgcagcat ctaaggctgc tttacgatca tctgccaata cgataatcct ccttatattt
    10321 acaaatcaga gcaacatttc attatcctta tcatatcttt tttagtgaaa aaaagcaaga
    10381 aaaaaccgaa caaatattcg catttatcat tttcttaaaa gctgttttct caaaagatca
    10441 aatccttgca taaccgcatt atatctgata gcagcacgat ctctggcaaa gtgatatttt
    10501 tccgaaacca cgccgtcttt agatgaaata gcgatccata ctgttcctgc agattgacct
    10561 tctaacgtgt ccggtccagc cactccggtg aaagctacac tgtaatctgt tcttgctatc
    10621 tcacgtgctt gaatggccat ctgctccaca cattctttgc tgactgttcc atattctgct
    10681 aatagctccg gatcaatctc cagaaaacca gcttttgttt ggagcgagta ggtaacgaat
    10741 ccgcctggaa atacttcaga tacaccagca atgtcgccta atgcggcttg gaaggcgccg
    10801 gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt ctttcagtag ttccactacg
    10861 acttttgcta gcgaattatc atcgccgtat ccataaaaat attcccctac tcgttcttgg
    10921 attttttctt ctaatgctaa aagagcttga ttccctgcct gaacatcatt tgttttcact
    10981 gtcagtcgta atgtcacttc atttggtttg gcatatggtg cgatcgttgg attgatctgt
    11041 gtctcaatca gatcttttag ctcagtgacc agtcgcgatt caccaatccc ataaaagcgc
    11101 aaaactttag aaattagttt ttcttcagaa ggaaaacgtt cctctagtaa aggacgtact
    11161 tgttccacaa acattggttt caactcatta ggcggaccgg gaagaaggat atacgcatgt
    11221 gtatctgttt gataaaaagt ccctaaagca agccctgtac gatttggtag aggaaccccg
    11281 ccttcgataa tctgggactg ttgcagattg tttttggtca tttttcgatg ggttgtttca
    11341 aaatatgcca ataatttttt gtatccttcc gtattttgta tgagagactt accgaggtga
    11401 gccgcagttg cttcttttgt caggtcatct tctgtcggtc ctaatccgcc acataagagt
    11461 atgagttcac ttcttgtttc agctagagca atcaactctt ctaaacgctg ctgattatct
    11521 cctacaactg tttgataata aacttcgata cctagatcag ctagctgttc tgacaagaaa
    11581 gtggcattcg tattcacgac ttgtcctagt aaaagttctg ttccgacagc tattatttct
    11641 gctttcatta cttcccgctc ccttatatag tatacgctgt atttttatat ttttttattt
    11701 aaaaagaggg caaggagctg tgacaaaaat cttgtcacag ctccttgttc caaataaaca
    11761 gtgttctcct gccaaaacct cggtcataag tcacaatata cataaaacat gaaaagctgt
    11821 tttttgaaat tatcccttgt gactcagagt tagacggcag gctcacaacc tctccactaa
    11881 acgatactta cattgatcct ttgaacacac cgctgttttt gatgaagtaa tccacacctg
    11941 aatagatagt aaagatcaga caagcataaa gcatgatttg atctactgga ataccgatca
    12001 ggctgaatgg gatattattg ataaacagca agataatagc aaccatttgt gtagccgtct
    12061 tgacttttcc cggccaagct gcagccatga cttctccatg ttcgacaagt agtaaacgca
    12121 agcctgtgac ggctaattca cgacagacaa tgatcgctac tacccaacca ggggcctttc
    12181 cttggtcaac caacatgata aaagcagtca taactagcat tttatctgcc aacgggtccg
    12241 caaacttgcc aaaattagtg acaaggcctc gcgcacgggc aatttttcca tctaaccagt
    12301 ctgtgatact agctaaagca aaaataactg cacctactag ctgtgtgaca gccagtgtgg
    12361 tgtctcctac gttaaattgt ccccaatcta aaggtaaacc tacaaccaaa atgaaaatcg
    12421 ggatcataca aattctaagt acagttaatt tatttggtaa attcacacta tcgactcctt
    12481 tcttccctat tctacgtgac tatcagcgaa gtttccaccg atattattta cttttccaaa
    12541 tgaatttatt caaaggagta tttttattga aattgatctt tttgaattaa cgaagaagga
    12601 ccgcgacaca gatttgtcac gatccttctt ttttatacct acaagtaaac agatgcgtaa
    12661 actaatttat acgatcattc tgcgtattga agagtaaggt tcacattttt tggactctga
    12721 ttgttttccc ctggattaac tggtacggat tctccattcg ctttgatcgt tgcattgctt
    12781 gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg gtaacacggt actctgtgtt
    12841 tcatttgctg ccagtgtccc ttgataaaca taggcattgt ctactagcac acctaaccat
    12901 acacgattga cagcagtaaa ttcaagggtt acaggcttgg tagcgtctgt gatatttact
    12961 gcgatggcac tgccagaatc ttctcctgta gctattgcca ttttcttttc ttcacttgat
    13021 tttgttgttg attctactgt agaagactct ttggtctgcg tgctttgctc tgaggaagag
    13081 gactcctcga ctgatccttc tactttaagc gaagaagctg tgtctccgat tattggatca
    13141 gcttgacgat cttgccaagt catatagccc acgacaacga caatcgccaa agcaaccaat
    13201 cctaataaaa tgacaggaag acttgtccaa aagcgacttg gatgcgattc ttcctgatgc
    13261 attgcttttc tagaagtact tactgtttca ggctgcggac gttcagggac cgatcctgtc
    13321 aatttgcttt tcccatcaaa aacatcgact aaatggtctc catcttctcc tacagcttca
    13381 gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag gcaattggtc aaaatcatca
    13441 ttgtcgattg cttccaaata acgtttttga atttttgtaa tctgctgtaa ttcatctaaa
    13501 gatatttttt tattcaggcg tgcttgccgt aatttttctc cgatggtttc aaaagccact
    13561 cgttcatctt ctccagtctg tttgattctc cgaagcatct gctgttgcaa cgaaagacgt
    13621 tatgtttggg tgctgtgaca aaagtcatgt cacagctcct tttttccgaa taaacattgt
    13681 tctcctgcca actcctcggt cacaagtcac aatatccaat aaatataaga agctgttttt
    13741 tgaacttgtt ccttgtgact cggagttaaa cggcaggctc acgacctctt tcttaaacgg
    13801 tgttcaatca gctgttcctc ggtattagct caaaaaccgg caaaatataa ggagatattt
    13861 ttccgtatgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac
    13921 ggtgttcaaa agcttgcatc tgcggtaata agtccaacca agttaaaaac attaagaagc
    13981 tgtttttact tggctgttct tattacttga tgcagggcag ctttttcaca acctcttggt
    14041 acacggtgtt ccagaagtag cttcttcggc aataagccga aatatcacaa aaatttgaga
    14101 agcaattttc gtaaattcct tcttatttc
//
LOCUS       DAZKMB010000071        13978 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000071 DAZKMB010000000
VERSION     DAZKMB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.071"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..460
                     /locus_tag="ACG8V3_001874"
     CDS             140..460
                     /locus_tag="ACG8V3_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300404.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACG8V3_001875"
     CDS             912..1112
                     /locus_tag="ACG8V3_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0300405.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ACG8V3_001876"
     CDS             1259..4048
                     /locus_tag="ACG8V3_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0300406.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ACG8V3_001877"
     CDS             4095..4622
                     /locus_tag="ACG8V3_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0300407.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ACG8V3_001878"
     CDS             4643..5833
                     /locus_tag="ACG8V3_001878"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0300408.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ACG8V3_001879"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ACG8V3_001879"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0300409.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ACG8V3_001880"
     CDS             7966..8640
                     /locus_tag="ACG8V3_001880"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0300410.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ACG8V3_001881"
     CDS             8637..8978
                     /locus_tag="ACG8V3_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300411.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ACG8V3_001882"
     CDS             9008..9367
                     /locus_tag="ACG8V3_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0300412.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9627..9749
                     /locus_tag="ACG8V3_001883"
     CDS             9627..9749
                     /locus_tag="ACG8V3_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300413.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9891..11975
                     /locus_tag="ACG8V3_001884"
     CDS             9891..11975
                     /locus_tag="ACG8V3_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0300414.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="ACG8V3_001885"
     CDS             12286..13752
                     /locus_tag="ACG8V3_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0300415.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4635 a         2430 c         2974 g         3939 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct acttctggaa
    13801 caccgtgaat cagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    13861 aaaaatagct cctcatattt tgccgttttt tgagctaatg ctgaaggtca gctgattgaa
    13921 caccatttat tcgaaacaag ggactttgac acgacttttg tcacagtccc ttgttttt
//
LOCUS       DAZKMB010000072        13959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000072 DAZKMB010000000
VERSION     DAZKMB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.072"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACG8V3_001886"
     CDS             <1..135
                     /locus_tag="ACG8V3_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0300416.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG8V3_001887"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG8V3_001887"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0300417.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="ACG8V3_001888"
     CDS             complement(2412..2663)
                     /locus_tag="ACG8V3_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0300418.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="ACG8V3_001889"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="ACG8V3_001889"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0300419.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="ACG8V3_001890"
     CDS             3613..3831
                     /locus_tag="ACG8V3_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300420.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="ACG8V3_001891"
     CDS             complement(4120..4632)
                     /locus_tag="ACG8V3_001891"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0300421.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="ACG8V3_001892"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="ACG8V3_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0300422.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="ACG8V3_001893"
     CDS             complement(7085..7528)
                     /locus_tag="ACG8V3_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0300423.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="ACG8V3_001894"
     CDS             complement(7529..8317)
                     /locus_tag="ACG8V3_001894"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0300424.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /gene="rnz"
                     /locus_tag="ACG8V3_001895"
     CDS             complement(8314..9255)
                     /gene="rnz"
                     /locus_tag="ACG8V3_001895"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0300425.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="ACG8V3_001896"
     CDS             complement(9432..10019)
                     /locus_tag="ACG8V3_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0300426.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="ACG8V3_001897"
     CDS             complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="ACG8V3_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0300427.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="ACG8V3_001898"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="ACG8V3_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0300428.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="ACG8V3_001899"
     CDS             complement(12936..13871)
                     /locus_tag="ACG8V3_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0300429.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3863 a         3126 c         2454 g         4516 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAZKMB010000073        13648 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000073 DAZKMB010000000
VERSION     DAZKMB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.073"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..266
                     /gene="yaaA"
                     /locus_tag="ACG8V3_001900"
     CDS             24..266
                     /gene="yaaA"
                     /locus_tag="ACG8V3_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0300430.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            253..1377
                     /gene="recF"
                     /locus_tag="ACG8V3_001901"
     CDS             253..1377
                     /gene="recF"
                     /locus_tag="ACG8V3_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0300431.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1374..3320
                     /gene="gyrB"
                     /locus_tag="ACG8V3_001902"
     CDS             1374..3320
                     /gene="gyrB"
                     /locus_tag="ACG8V3_001902"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0300432.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3340..5811
                     /gene="gyrA"
                     /locus_tag="ACG8V3_001903"
     CDS             3340..5811
                     /gene="gyrA"
                     /locus_tag="ACG8V3_001903"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0300433.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6047..6346
                     /gene="rpsF"
                     /locus_tag="ACG8V3_001904"
     CDS             6047..6346
                     /gene="rpsF"
                     /locus_tag="ACG8V3_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0300434.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6396..6908
                     /gene="ssb"
                     /locus_tag="ACG8V3_001905"
     CDS             6396..6908
                     /gene="ssb"
                     /locus_tag="ACG8V3_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0300435.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6934..7170
                     /gene="rpsR"
                     /locus_tag="ACG8V3_001906"
     CDS             6934..7170
                     /gene="rpsR"
                     /locus_tag="ACG8V3_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0300436.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7313..9286
                     /locus_tag="ACG8V3_001907"
     CDS             7313..9286
                     /locus_tag="ACG8V3_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0300437.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9292..9744
                     /gene="rplI"
                     /locus_tag="ACG8V3_001908"
     CDS             9292..9744
                     /gene="rplI"
                     /locus_tag="ACG8V3_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0300438.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10018..11385
                     /gene="dnaB"
                     /locus_tag="ACG8V3_001909"
     CDS             10018..11385
                     /gene="dnaB"
                     /locus_tag="ACG8V3_001909"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0300439.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12165..13457
                     /locus_tag="ACG8V3_001910"
     CDS             12165..13457
                     /locus_tag="ACG8V3_001910"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0300440.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4544 a         2475 c         3007 g         3622 t
ORIGIN      
        1 ataatgaaaa tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga
       61 cgttgggcca aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt
      121 atttggcaga aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac
      181 tttatcccgg catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag
      241 gaaacgacga taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag
      301 ctgaacatcg aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa
      361 acgaatcttt tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt
      421 gagcaggaat tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa
      481 ggttcatcca ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc
      541 aaccatatcg aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt
      601 gcaccagaag atctttcttt agtaaaaggc agcccgcaga tcaggcggaa gtttttagat
      661 atggaattag gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt
      721 ttgaaacaaa gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt
      781 tatttggata ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga
      841 gcacagttcg tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat
      901 caaaaagagc agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg
      961 caagagcaaa tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt
     1021 tttcggggaa caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag
     1081 aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa
     1141 ttagcagaaa tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat
     1201 gatgtgatga gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga
     1261 aaagttcaaa cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt
     1321 gaacctgaga ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag
     1381 aagaaagaag tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg
     1441 aaggacttga agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg
     1501 gtctgcatca ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt
     1561 ttgcaacgaa aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg
     1621 gtcgggggat acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct
     1681 ttactgtttt acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac
     1741 ttcacggagt aggatcttct gttgtaaacg cgctttctac acagcttgat gtcaaagtat
     1801 acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa
     1861 agattatcga acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa
     1921 tttttacaga gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg
     1981 ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca
     2041 tcaatgaata ccattatgac ggcgggatca aaagttatgt cgaatacttg aatgcaaaca
     2101 aaaccgtgat ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg
     2161 aagtctctat gcaatataca gacggctacc attccaatat catgagtttt gccaacaata
     2221 tccatacgta tgaaggtgga acacatgaat caggattcaa gactgcttta actcgtgtga
     2281 taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg
     2341 aagacgtgcg agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg
     2401 aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat
     2461 ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgg caaattgttg
     2521 aaaaaggatt actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga
     2581 gacgtaaagg tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca
     2641 acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta
     2701 agcaaggtcg taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg
     2761 ttgaaaaagc aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg
     2821 ctatgggtac agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag
     2881 ttatcatgac tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt
     2941 tttatcgtta catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt
     3001 tatacggggt aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag
     3061 aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt
     3121 ataaaggact tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca
     3181 atcggatgat ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa
     3241 tgttgatggg ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg
     3301 tgaagaactt agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa
     3361 aacatccatg atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg
     3421 agtgttattg tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc
     3481 cgtattttat acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct
     3541 gccagaatcg tcggagatgt catgggtaaa taccatcctc atggagacat tgcgatctat
     3601 gagtcaatgg tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat
     3661 ggaaactttg gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg
     3721 atgagcaaaa tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa
     3781 agcaactatg atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt
     3841 ttagtgaacg gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac
     3901 ttgtctgagg tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat
     3961 gaattgatgg aagtattacc tggaccagat tttccaacag gtggtttggt tatggggaaa
     4021 tcaggaatcc gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt
     4081 gagattactg agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg
     4141 gtcaataaag caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa
     4201 gggatcacag atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc
     4261 cgtcgtgacg ccagcgcatc cgttatcttg aataacttat ataaattgac atccttgcaa
     4321 aattcatttg gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg
     4381 aaacaaatcc ttgaaaacta tatcgaacat caacgcgaag tcatcacacg acggacggtc
     4441 tttgaaaaag aaaaagcaga agcaagagca cacatcttag aaggtttgcg tattgctttg
     4501 gatcatatcg atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa
     4561 gcaacaatga tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg
     4621 cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc
     4681 ttaaaactga ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc
     4741 atcaagaatg aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta
     4801 ctagtcggtg aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt
     4861 attacattga caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa
     4921 cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat
     4981 ctggtttctt gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat
     5041 cgggcaaaag gatacgaaat tccagaatac gggcgtacag ccaaaggaat cccaatcatc
     5101 aatttactag gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa
     5161 gcagaagaag gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct
     5221 gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac
     5281 gatgagctcg tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat
     5341 aacggctact ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc
     5401 ggggtcagag ggatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag
     5461 gaaaatcaag aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg
     5521 gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa
     5581 aatggtccac tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca
     5641 gataaaggtg tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca
     5701 ttaggcgtac gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc
     5761 gaaccagaag ctgcagaaca aacagttgaa gaacaaggat cagaagaata accatcaaga
     5821 ctaagtgtgt aagctagcaa gaataaccga ttaaaaaatc tggttattcc tgctggcttt
     5881 tttgattagt tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac
     5941 caaaatagag tgtataatta gagcttgtga gtagtctaat taattagata actctccttg
     6001 ctcttaggaa cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa
     6061 atatgaaatc atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat
     6121 cgaacgtttc gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg
     6181 ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt
     6241 taaagtatct tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat
     6301 caacgacgat atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt
     6361 gaaacaatag aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg
     6421 gacgtctgac gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat
     6481 ttactttggc tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta
     6541 tcaactgtgt gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa
     6601 cacttttagg tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac
     6661 gtgtatacgt gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat
     6721 ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg
     6781 gcggaaacaa taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc
     6841 gtgatacttc tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac
     6901 cattctaaac gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt
     6961 aaacgtcgta aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat
     7021 atcgaattac taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact
     7081 ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg
     7141 ggcttgctac ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt
     7201 ttgatcagct attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc
     7261 gaatgacgaa agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa
     7321 taaaaacaat catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt
     7381 attggtagtc ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa
     7441 tttatggggt gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga
     7501 aaaaatccag caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat
     7561 gcctgtagga gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt
     7621 agcattgaaa atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt
     7681 tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga
     7741 tatggataca gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa
     7801 actccatcag acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga
     7861 tgccattgat aaattagatg acaaagagat ttcttatttg aactctttcg tcactacact
     7921 agtttctgac tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt
     7981 tttcttcgta gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct
     8041 ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat
     8101 tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt
     8161 tgctttagtc cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc
     8221 tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc
     8281 gatgagtacc gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca
     8341 caagtttccg gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt
     8401 caaccagaaa aaagcttaca tcgttatcaa tgagaacgag atcattccag atgtagaacg
     8461 gtgtcttgaa gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg
     8521 tgccatagag ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc
     8581 tctatcgatc tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca
     8641 tcgtcgtggg gaagagtttc caagtaaacc tctgctgact tatattgaat cttcagcttc
     8701 ttcagcttca gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt
     8761 agatcgtttt gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa
     8821 tgtccgtaca actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga
     8881 cgcatcctta gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa
     8941 tttgattgct ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga
     9001 aaaagagtat aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa
     9061 tatcaatgca gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag
     9121 aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt
     9181 cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt
     9241 tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta
     9301 atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca
     9361 ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt
     9421 atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa
     9481 gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg
     9541 aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg
     9601 ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc
     9661 cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc
     9721 aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa
     9781 ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca
     9841 aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca
     9901 taaagaaaac gagtataata aaagagaact atttttttat tacactttcg gtacgaatga
     9961 tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg
    10021 aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta
    10081 ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa
    10141 gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt
    10201 aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa
    10261 gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat
    10321 atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca
    10381 gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat
    10441 gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct
    10501 attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa
    10561 gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag
    10621 cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg
    10681 aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa
    10741 atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc
    10801 catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg
    10861 agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc
    10921 cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat
    10981 tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc
    11041 tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag
    11101 ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa
    11161 tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat
    11221 gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa
    11281 gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa
    11341 gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag
    11401 ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta
    11461 gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct
    11521 ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa
    11581 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac
    11641 gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca
    11701 tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc
    11761 tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca
    11821 aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc
    11881 tttttcacaa cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa
    11941 atttcacaaa aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc
    12001 acttctgtta caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag
    12061 cgtttttata aaataaaaat aaaaatcatt cgattttatt tgaaatatgt atctattatt
    12121 gatacaatgg ttcaggatta aataatatag aacgaggtgt tcacatgtca tctgtagtag
    12181 tagtaggtac gcaatggggc gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa
    12241 atgctgaagt gattgcacgg taccaaggtg gcgacaatgc agggcacaca attaagttta
    12301 acggtgtgac ttataaactg catttgattc cttctgggat tttttataaa gacaagatca
    12361 gtgtgatcgg aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt
    12421 tacatgaaaa aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct
    12481 tgccttatca tatcaaattg gaccaattac aagaagatgc taagggcgaa aataagattg
    12541 gtacaacgat taaaggaatc ggacctgcct atatggataa agcagctcgt gtaggtatcc
    12601 gtattgcaga cttattagat aaagaaattt ttgaagaacg tcttcgtttg aatctagatg
    12661 acaaaaaccg tcaatttgta aaaatgtttg attcagaacc acttgatttt gacgatattt
    12721 ttgaagaata ttatgaatac ggacaacaaa tcaaacaata cgtcacagat acttccgtta
    12781 tcttgaatga tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa
    12841 tgctggatat cgaccaggga acttatcctt ttgttacttc ttctaaccca gtagccggtg
    12901 gggtaacaat cggaagcggt gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca
    12961 aagcctatac gtcacgtgtc ggtgatggac catttccaac tgaattattt gatgaaacag
    13021 gagaacaaat ccgcgaagta ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg
    13081 taggctggtt tgattctgta gttatgcgcc attctaaacg agtttcaggt attaccaacc
    13141 tatctttaaa ctcgatcgat gtattaagtg gattggaaac agtaaaaatt tgtacggctt
    13201 atgaattaga tggcgaacta atctaccact atccagcaag cttgaaagag ttgaaccgct
    13261 gcaaaccaat ctatgaagaa ttgccaggct ggtcagaaga cattacagga tgcaaaacac
    13321 ttgcagaact gccagaaaac gcacgtaact atgtacgtcg tatctcagag cttgttggtg
    13381 tacgtatctc aacattttct gtcggacctg atagaacaca aacaaacatc ttagaaagcg
    13441 tctgggcgca aatctaagga taaaaacgac gactgaatca ggacgttctt accagagcga
    13501 gtcgaagtgt ttatctatac gataataata acttgaagag gtagtgaaag agacgtttag
    13561 ccttattgcc gataggctgt ctcttgaaca ctgtttattc agaataaggg actgtgacaa
    13621 aaaccgtgtc acagtccctt tttccgaa
//
LOCUS       DAZKMB010000074        13240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000074 DAZKMB010000000
VERSION     DAZKMB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.074"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..1946
                     /gene="yfcC"
                     /locus_tag="ACG8V3_001911"
     CDS             207..1946
                     /gene="yfcC"
                     /locus_tag="ACG8V3_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0300441.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2103..3287
                     /gene="iadA"
                     /locus_tag="ACG8V3_001912"
     CDS             2103..3287
                     /gene="iadA"
                     /locus_tag="ACG8V3_001912"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0300442.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3398..3601
                     /locus_tag="ACG8V3_001913"
     CDS             3398..3601
                     /locus_tag="ACG8V3_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0300443.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3702..4364)
                     /locus_tag="ACG8V3_001914"
     CDS             complement(3702..4364)
                     /locus_tag="ACG8V3_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300444.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4377..5879)
                     /locus_tag="ACG8V3_001915"
     CDS             complement(4377..5879)
                     /locus_tag="ACG8V3_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0300445.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6427..8106
                     /locus_tag="ACG8V3_001916"
     CDS             6427..8106
                     /locus_tag="ACG8V3_001916"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0300446.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8233..8712
                     /locus_tag="ACG8V3_001917"
     CDS             8233..8712
                     /locus_tag="ACG8V3_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0300447.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8931..9611
                     /locus_tag="ACG8V3_001918"
     CDS             8931..9611
                     /locus_tag="ACG8V3_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0300448.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9619..10950
                     /locus_tag="ACG8V3_001919"
     CDS             9619..10950
                     /locus_tag="ACG8V3_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0300449.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10965..11927
                     /locus_tag="ACG8V3_001920"
     CDS             10965..11927
                     /locus_tag="ACG8V3_001920"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0300450.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12038..12376
                     /locus_tag="ACG8V3_001921"
     CDS             12038..12376
                     /locus_tag="ACG8V3_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0300451.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12455..13162
                     /locus_tag="ACG8V3_001922"
     CDS             12455..13162
                     /locus_tag="ACG8V3_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0300452.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4235 a         2326 c         2895 g         3784 t
ORIGIN      
        1 attttaagga aaaacagaga aataaaatac ttaatataaa tggaaagaga aaaaaataat
       61 catacatagg agaaaaaaaa caaaatagaa aaaagtgatt ttctaataaa ttattcatgt
      121 ggaaatgtca tttttactgt ataattcatt tgattatcca ttttttattc atcaaaatta
      181 ctgaaaaaag aaaggaagaa ataaatttga agaaaacaaa ttcttcagga aaaccgaaac
      241 gcgacttcac taaattatcg acaccccata cgtatgtcat tatttttggt gtagtcatct
      301 ttgcatggat actgactttt gtcgttccag caggaaagtt cagcacgcaa gatattgaat
      361 acaaagatgc aaatggcgaa acaagtactc gtactgtttt acgtcaagat tccttccgtt
      421 atgcttacga gctggataag tcgtatgtgt ttgatcaatt agaagaactg caagatcatc
      481 cagcagaacg tgaaaaactg gatgttccgg aaaaaggatt ggaaaaagtc attgctgacg
      541 gagaaaaaaa tcttacgcag gaaaaactag atgaaatctc cttgacagat gatgtgcttt
      601 acgatcagta cggcgaaaat atctatgata cttctaaaaa attgcataaa acagccaaaa
      661 tatggggaac agatgatttt ggcggctttg gtttcttaaa ttttgttttt gaaggactag
      721 tatctggaga taagtatgga tctgctgtag ggatcgctgc attgatcctt gttgttggag
      781 gtgcttttgg tattattatg cgtaccggag cgatcgacgc aggtatctat gcgtttatca
      841 gtaaaactaa gggactggag cgtttagctt taccgctatt attctttgct ttttcattcg
      901 gtggcgctac attcgggatg gcagaagagg taattccatt ctcaatggtg atggttccat
      961 ttgtcattgc gttaggatat gactcgatcg tagcagtaac cgtgacttat gtcgcatctc
     1021 aagtcggaaa tgcgacctct tggatgagcc cgtttagcgt agcggtggct caagggattg
     1081 ctggaatccc cgtgttgtct ggtgcgacat tccgattgat tatgtgggta gttgttactg
     1141 cattagccgc tggttatatg atgatatatg cagaaaaaat ccgtaaaaaa ccagaaagtt
     1201 cgttgactta caaatcagat gattatttcc gttctcatat caaaaagaca tctgatgaaa
     1261 ataaaaaatt catgcttggg cataaattga tcttattaga gatgttagtt gtccttgtct
     1321 ggatcgtttg gggcgtgact caaaaaggct attacattcc agaaatcgct tctcaattct
     1381 ttgtgatggg attagttgct ggaatcattg ctgttctttt caaattagat ggaatgaaag
     1441 tcaatgacat cgcttcttct ttccaatctg gtgcagctga tttagctggt acagcaatcg
     1501 tagtaggtat ggccaaaggg attcttcttg tcttgggagg atctgatgca tctgttcctt
     1561 cagctttgaa tacgattttg catgggatcg ggacggcgtt gaccggtgtt ccagctgtca
     1621 ttggtgcttg ggcgatgtac atcttccaaa gtttgttcaa cttggttgtt acatcaaatt
     1681 caggacaagc agctttgact atgccaatca tggctccact ggcagattta gtaggtgtat
     1741 ccagacaagt agcagttctt gcttatcagc ttggttcagg ttttatggac gcttttacac
     1801 ccgtatcagc cagcttgatt ggtgtgctag gtgttgcacg gatcgaatgg gcaaaatggg
     1861 caagattcca aatcaagatg caaggattcc tgtttgtttt aggaactatt ttcatcatga
     1921 ttgccattgc aataggtctg cagtagtact ggagacctgt aaataaaaaa gtaatccaaa
     1981 cagtagataa ggactccaag aaccgtcatt ttacggtaaa atcaaaggag taaagatcct
     2041 atcgtttgga ttatttttta agatcctatc atcagttata cttaaagaaa gaggaaaaat
     2101 gtatgaaaat cattcgacaa attgaagtgt ttgcaccaga ttacttagga aaaatggatg
     2161 tactgattgc cggtgggaag attttggctg tagaagaaca gctagaaggc ggctacgaag
     2221 gagtggaagt agaagaactt tcaggtgagg gaaaagtact aaccccgggt ttcattgact
     2281 gtcatttcca tattttaggt ggaggaggag aaggcggtta tcaaaatcga acgccagaag
     2341 tcacattgag ccaattgact acagctggtg taacgacagt tgtcggttgt ttgggaacag
     2401 acggagaagg tagagatatg actgcgttga tcagcaaagc gaaaggtctg gaagcagaag
     2461 gaatctccac ttatgtatac gaaggttcgt accgattgcc ggtaaaaaca gtaacagatt
     2521 ctatcatcaa agactttttg acaattgata aaattatcgg aatcggagaa atcgcagtat
     2581 ccgatcaccg ttcttctcag ccaagttttg aagagttcgc tcgtgcagta gcagatgctc
     2641 gtgtaggagg catgttatcc ggaaaagctg ggatcatcaa tgtccactta ggcggaggaa
     2701 aaagaaagct agagctattg acacgaatcg tagaagaaac agaaattccg atcacacaat
     2761 ttttgcctac tcatgcaaat cgcacaccag aattatttga agcttgcgtg gcatttgcca
     2821 aacgtggtgg cacgattgac ttcactgcta gcgaagatcc tgatttttgg gagaagacag
     2881 atggtgaagt tcgcttcagc aaagcactga aacgattgat cgaggaagat gtttctttag
     2941 ataatttcac gatgacctca gatggacaag gaagcttgcc ttattttgat gaaaacaatc
     3001 atttcctagg gttaggagta ggaagtgcca aggcattgct agtagggatc aaagaagctg
     3061 tgcaaaaaga aagtatccct ttggaaatcg ccttacgagc tataaccagt aatccagcac
     3121 gtatattgaa attggataaa aaaggaaaaa tcgaaattgg tgctgatgca gatctgtgta
     3181 tcttggataa agaaacgctt gatattgata cagttatcgc taaaggagaa ataatggtcc
     3241 aagaaaaaga agtcaaagta tgggggactt tcgaaaaaag tttttaatca acagctctaa
     3301 ctatcttttt tagaaaacga gcgttctgtt tgtctgtatc tgttgttgat gttaccctga
     3361 aggtagatca ataaacaaac taagaggagg aagcacaatg gcagatttaa aaggacgttc
     3421 taaagatatg aaagataaag tagtcggtga aggaaaagaa gcatacggca aagcaacaga
     3481 cgacaagagc aaagaaacag aagggaaagc acaatcacta ggtgctgaca tcaaagaaaa
     3541 agctcgaaat ttaggcgacg aagtcaaaga caagttttct cgaaaagacg aaaaaaaata
     3601 aaaaccactt attactaatt gaatcaagca aataaaaaat ccgaaccata tctgcgagac
     3661 agatatggtt cggatttttt atttgatcat tatcctgttt tttaaacttc gattttttga
     3721 aattttttat tttttagttc aaagacttca tcacagcttt ttgctacttc aatctcatga
     3781 gtcacgatga taatacagcg attttgttca tgggcgattt cttggaaaag tttgacgata
     3841 tcttgtgaag ttgtttcatc tagatttcca gtaggctcat ccgctacgat tagttgatgt
     3901 tcacagcaga tagcacgaac gatagcgact cgctgctgct gacctcccga aagtttggtc
     3961 acctgttttt ttgctaatat ttcatcaatt cctacacgag ccagtcgatc taaagcaact
     4021 tgtcgataat tctcttgctt ggtcttagtg atttccattg ctgctatgac gttgtctaga
     4081 gccgataaat aaggaagcaa gttgtaagct tggaaaataa tcgagacatc atttttacga
     4141 taacttctta acccgatttt gttcagtgaa cgatcttgat agtatacctt tccttctttt
     4201 ggtgcatcta gtccagaaat aagcgaaaga aaagtcgttt ttccagaacc acttgctcct
     4261 aaaatagcgt acatttttcc agcttcgaaa ttcagttcta catctttata aagcgcatct
     4321 tgcggatctt gataccaaaa tcccaattgt tctgtccgta acatcaaatt cctcctttag
     4381 tttaatagga tttttcttgg gtttaatctt aatatcccta tagaagctaa aaagacagaa
     4441 aacagactga ttccaaatgc tactccggct aatagtccca attgtgcagg tttgacattg
     4501 atttccaatt cgctgacttg atcagaaaca gcaaatggat tattcgtggt tccttgttga
     4561 ccatcgcgtt gaccgccttg ttcttgtctg ttacctggca tttgtccacc gccaggagct
     4621 tgctggtttt gtgtcgtggt ttctgtctgt tggtcaagta gctgctgtcc cacggcattc
     4681 gcgacgacgt ttccgctaag cgaagcaact cctaatgcaa gaatcataca aatagccact
     4741 tcggtaaaca attggagaat gattttactt ctggattctc ctaaagatag caagacgcct
     4801 aactcatgcc gacgctcccg aatggtaatc ataatgatca gagtaagtat gataataccg
     4861 gctacagcca ctagtaaaac aacattttta gaaaaacttg ccacgttgtt taaaggttcc
     4921 aacattgact gatacatgga atcatttgat tgtaagctaa atgtatctgt gtcgatcagc
     4981 ttctcagctt ctttgagaaa ctcatccatt ttatctggat cagatagagt gtaaatagca
     5041 gaatctatcg tgttatcgtc agatgtccca tttagttcat ttgcaaaagt gtaagaagtg
     5101 taaagtgtat tcgacggatt catgaagttg aatttcatac ccatactgcc gcttgtttct
     5161 ctggattcat aaattccttt gatcgtcatt tcataagtgg tatcttcatc ttcagtactc
     5221 gtgattgtga aagtatctcc gactgccagg tcatttgttt ctgctagagt agaatcaatg
     5281 actacattgt ttgtgccttt gtcgctgtca tctattcctt caccatccgt gatttttgct
     5341 gttccatcag aaaaattact attttgtgca gttgctgaca caccactgat ttgaaagtct
     5401 gcttgagtca tttgcggatt cccgcccatt cctccagcag gttcaccgcc tgcatcagta
     5461 gtttctgaac tgtcttcact tgaatcagaa ctagtgatgg cagttattcc atcttttgcc
     5521 atagctgagg cggaactttc aaaggaataa ctctttacat tatctaattc agctattttt
     5581 tccgcgtccg ttaaggaaac tggtgtaaga gaaaaactgc ctggatctgg acgttcttca
     5641 gtggtagaac cgcttgaaga agtttgttcc tgttttttaa atgaagcttc tcgattggca
     5701 cttaaagtca ctgttgctcc aacttctttt tgtgcttgat tcgctgcttc ttctgcagcg
     5761 cttcgaatcg tgaggccagc tagcacaaaa ataagaatgg ctgtgaacac ggcaaacaat
     5821 aacaatgaac gtccccattt tacttttgta cttttccatg cacgtttcat aaaattcata
     5881 ttttgcatcc ctcctagtag atgcatttga taatagacaa cctatcttaa agctagctta
     5941 aatcttatgc tgtttgcaca ccatcaacat gaggttctgt atatataaaa agtcactata
     6001 gttattgcaa aaaatgtggt aaaatgaaaa aagtatgttt caaaccgacg aagaagaaat
     6061 gaaagaggaa tagcaaaaga aatcctagat gctacagagg gatttttacg tgcatagttg
     6121 ttctaacaat tgctgatact agcgatttag tttatttgtt gtataataat ggggaacagc
     6181 tgaatactta tataagtatt ctggctatag cgtttcgcaa aagatccatt ttgttaatga
     6241 agtatatcca aaataaaatg cccatccaac tgtcagaacg cggatgtgct ggtaattttc
     6301 ctgctgattc tccataagtt gagaaatagt gattttgcat atgcttcatt ggaatggctc
     6361 atatatttag ataaagaaga aaatcgacat ctttaaactt ggatgtcata agaagaggtg
     6421 aagtttgtga gtaccattaa aattatccca ttaggcggcg ttcgtgaaaa tggaaaaaac
     6481 atgtatgtcg cagaagtggg cgaggatatt tttattttag attgtgggct aaaatatcct
     6541 gaaaatgagt tgttggggat cgacgtcgta atccctgatt ttacgtattt agaagaaaac
     6601 attgatcgtg tagctggtgt ctttttaaca catggacatg cagatgcgat cggggcactt
     6661 ccttacttgt tgtcaaaaat ttctgtacca gtattcggga cagagttgac gatcgagtta
     6721 gcaaaattga atgtcagtcg taatgaagca gccaaaggat ttaaggattt ccatgtggta
     6781 gatgagcata cagaaataga ctttggtgaa acagtagtga gctttttccg taccactcat
     6841 acgataccag attcagtggg gatcaatctg aaaacaaaag aaggtagtat cgtttatacg
     6901 ggagatttta aatttgacca aacggctatc ccaatgtatc aaacagatta tgcacgattg
     6961 gcggaaatcg gcaaagaagg agtcttagcc ttactaagtg aatcggcaaa tgcagaaaat
     7021 cctcagcctg tagcttctga agcccaaata gcagatgaag tatttgatac gatcaaatat
     7081 tgggaagggc gtatcatcgt cgcttgtgta gcaagtaatt tgcaacgagt gcaacaagta
     7141 ttggatgcag cagaaaaagc acaacgaaaa gttgtcttga ctggtcaaga tttcgaacga
     7201 attattcgca cagcgatgcg tttagaaaaa cttcaattac caagtgaaga tctgcttgtt
     7261 acgttgaaag acatgaaaaa ctatgatcca gaagaactga tcattttaga aactggccga
     7321 atgggtgaac ccatcaagtc tctgcaaaaa atggcaaacg gtacacaccg taatgtcaag
     7381 attgaagaag gagatttggt ttatatcact actacgccaa gcatcgcgat ggaaaccatc
     7441 gtggcgaaaa cggaagatat catttatcga gctggtggaa cagtgaagtt gatctctgag
     7501 aatatgcgcg tttctggcca tgccaatcct aatgacttgc aattaatgat caatttgatg
     7561 aaaccaacat attttgtgcc agttcaagga gagtatcgtg aattagctgc tcatgcagat
     7621 ttggctcatg cagtagggat gccatacaaa aatatctata ttactggacg cggagacgtg
     7681 ctggaatata agaacaagcg tatgagtgtt gcaggaacta caagtgcaga aaatgtaatg
     7741 attgatggat taggcatagg tgatatcgga aacattgttt tacgtgatcg aaaaatcttg
     7801 tcagaagacg ggattttcgt agcggttgtc actatcagtc ggcgagaaaa gaaaatcatt
     7861 tcaaaacctc aaattacttc acggggattc gtttacgtaa aagctagccg tgatctgatg
     7921 aaagaaagca gcaatatgat tgaaacaatc gttgagaaac atttagaaag caatgatttt
     7981 gagtggagca aattaaagca agacatccga gatcaattag gccgttactt atttgaacaa
     8041 acaaaacgcc gcccagtgat cttgccagtc attatggaag ctactcaacg tcgaaataaa
     8101 aaataaacaa aaagagtaag atgaacctct ctatagagtg ctcgtcttgc tctttttttg
     8161 cgaaaaacaa ataatttttg aatacagcat ccctcgcttt ataataaacg atagaataaa
     8221 gggagtgaat caatgatgag tcaaacaaaa gtcgaacgtt atttagaaga acaaaagatc
     8281 agctatcaac cacttgattt tagtcatgtg gtagaagaat ctttcacaga agaactacaa
     8341 aaaagaggga ttgatccagc tttgatatgt aaaactctcg tagtgaaagg aaacaaaact
     8401 ggggtgatca tcgcattgat tcctttgaac gatcacttag attacaaaaa aacgagacaa
     8461 gtgacgaaag atcgtaaagt tggttttcct ggaatggatt ttgtattaat gcatacagga
     8521 tatccccacg gagcaaacac ccctattgga ataaaactcg cacatcctga ttattgtttt
     8581 ttagcagaca gttctttaaa agaaaaagaa gaagtaattg tgtcaagcgg tgaaatcggg
     8641 agaagccttc ggataaaagt agccgattta gaaaagatcg tccatcctat ttacgctgat
     8701 ttaattagtt aagggacgag aagagaagct aagcagagca gagacctcaa acttgagatt
     8761 atatctgctt ggtacttggt gcatcctgta ttatctgtta caattgcttt gtacatactg
     8821 tactatcaat ccaatgaaaa tatatttagc agctatattt tttcattgat gtgcaagtga
     8881 tgaaatcgtt tttaataaaa aagatacaga gaaaacgcga ggtgtaaaat atgaaaatta
     8941 cgtatcatgg acattcctgt ttaacaattg aaatggaaaa tggccaacgc gtgatgattg
     9001 acccgtttat tacaggaaat ccattgacag acttaactgc agaagaagta aagacagatt
     9061 ggttgctgat tactcatggt catagtgacc acataggaga tatgattcct attgcaaaag
     9121 aaaatcaagc cacaattatc agtattgttg agattgctga ttatgcgaac agcagaggag
     9181 tagattcatt cggtatgaat atcggtggaa aacatgcgtt tccattcggc gtggtaaaat
     9241 tcgtacatgc acagcatagt tcaagctatg aagtagacgg tatcgttcag tatatgggcg
     9301 agcctagcgg aattattatt caagcagaag gaaaaacgat ttatcatgca ggggatactg
     9361 cttatttcag tgatctagga ttgcttgcag aagaattcga tattgatgtc gctttcttgc
     9421 cgattggcga taactatacg atggggccag aagatgcagc aaaggctgcg caaacaattc
     9481 gtgccaagaa agtagttcca atccattaca atactttccc tgtcattgaa caagatcccg
     9541 aatcttttgt agagttactg gaagatagtg aaggaaaaat aatgagcgtc tcggaaacgc
     9601 tggaagtgta ggcagaaaat ggcgacaaaa catgatttga ttttagaata tattgaaagt
     9661 ttacctgttg gcaatcgaat ttctgtaaga agcatcgcaa aagaattgaa tgtcagtgaa
     9721 ggaacagcgt accgtgcaat caaagatgca gaaaatgtcg gactagtttc aacaattcaa
     9781 cgagtaggga ccatccgtat cgaacgcaaa ttaaaaaaac atattgaacg tttgacattt
     9841 ggcgaggtcg tcaggatcat tgaaggggac gttttaggcg gggcggcagg attagacaag
     9901 gtcttgaata aattcgtcat cggcgcgatg actaaacatg ccatggaacg ctatattacg
     9961 ccaggatcac taatgatcgt cggcaaccgg gcggaagtcc aaaaattagc attggaagat
    10021 ggcgccgcag tattgatcac aggaggattc aatacgactg ctgagattgc aaagctagct
    10081 gatgatcttg aaatgccgat cttacgcacc tcttatgata catttacagt cgcaaccatg
    10141 atcaaccgag cgttaagcga ccaattgatc aaaaaagata tcatgcttgt cagtgatatc
    10201 tatatgccag ctgaaaagac tcattatttg cagcagacag atacgatttc tgattatcag
    10261 cgtttgtcag aagaaacgca gcattcgcgt tttccagtcg tcaaccgcca tcatcgtttg
    10321 atggggattg ttacggcaaa agatgtttta gggaaatcgc caaaccagat catcgatcgg
    10381 gtgatgacga aagaaccaat cagtgtaaag aaaacaatga gtattgcctc tgttagccat
    10441 caaatgatct gggatgggct agaagtcatg ccggtcgttt ccgatgactt gacattggaa
    10501 ggcttgatta cccggcaaga tgtaatgaaa gcgatgcagc ttgtccagcg acaaccgcag
    10561 attgcagata cgatttcgga tcagatatct ggaaatatcc atacgattga tactgaccgt
    10621 gaaggaaatc gtttggaaaa ccctaagttc aaattcgtag tagcaccaca aatggtcaat
    10681 ggcgttggaa ccatttcttt tggcgtactt agcgaaatca tttctgatgt cgcacaaaaa
    10741 acaatggtga tgaaccaaaa acgaaatatc ttgattgaac aagtgaacct acattatttg
    10801 cggctgatcc aattggaaag cgaattagac attcgtccac gtgtattgga aatcggcaga
    10861 cgttccgcta aactggatat cgaagtgttt attgaaaaca ccatcgtttc caaagcaatc
    10921 gttgtttgtc aagtaatgga acgttcttag aaagcaggag tgaaatgaca gtacagcaag
    10981 aaatagtaga tacaataaaa aaatacgata aaatcattat ccacaggcat ttgcgccctg
    11041 atcctgatgc attaggatca caagtaggat tagccgaact attacgggaa agtttcccag
    11101 aaaaagaaat acgtcaagta ggtgaaacga ttgaagggct tcgcttttta gcagaaatgc
    11161 aagaagttga agatgtcttc taccaaggag cgctagtcat cgtgactgat acagctaatt
    11221 cacccagaat cagtgatcaa agatatcagt tggcagaaaa actgatcaaa atcgaccatc
    11281 atcccaatga tgacccttat ggagatcttg tgtgggtaaa tacaaaagca agtagctgta
    11341 gtgagatgat tgcagaattt gctttgatgt ttcctgaatc tttaaagatg aacgttaagg
    11401 ctgcacggtt actttatgca ggaatcgtcg gagatacagg gcgctttttg tatccctcca
    11461 cgacgtcccg tacattagag attgcttcga tattgagaaa ttatccattt gatgcaagtg
    11521 ctttgaacag agagattgaa caaatgccga tgaaagtcgc taaactttcc ggttatcttt
    11581 atcaaaatat ccaggtggat gaaaatggtg caggaagaat aatccttccg caagaaattc
    11641 ttgataaata tggaatcgat gattcagaga cagcttcgat cgtttcactt cctggagtga
    11701 ttgacgaagt gctggcttgg gggatttttg ttcagcagcc ttcagggtat taccgcgtac
    11761 gtttacgttc taaggggccg atcatcaatg aattggctaa acgccatcat ggaggaggcc
    11821 atccattagc aagtggtgcg aatgcaaaag atttagcaga agtagaggaa atctataaag
    11881 agatccaaga tatatgccaa tcgtataaga gcagaaaaac agaatgatac ttcgttcgaa
    11941 aacgtgcctt ttgtagtaaa aatcacaaat gtatcttttc aagatcttct ttttccgtta
    12001 caatagtgct cactaaagat tttaaaggag aaagaagatg acattaccaa attgcccaga
    12061 atgtggctca gagtacacat atgaagatcg aggcttgttg atttgcccag aatgcgggaa
    12121 cgaatggaac aaagaagaac aagaaactgt catagaagac ggattagtgg tattagatgc
    12181 aaatggaaac cgtctgttag agggcgatag cgtgacagtg attaaagact tgaaagtaaa
    12241 aggtgcgtca ggtgccatta aacaaggaac aaaagtcaag aacatccgtt tagtagaagg
    12301 cgatcataat atcgattgca aggtagaagg atttggaccg atgaaactga aatcggaatt
    12361 tgtgaaaaag aattaaagaa tattcaaggt cttttcacat tcttttccta ctactaatga
    12421 taaaataaag taaacgaaaa ggagtgaaac gaaaatgtat ggttatggaa tgtatggttt
    12481 tttagatccg acatttcttt tggtcattct tggattagtt atttccggaa ttgcgtcagc
    12541 gtacgtcaat agtactttcc gaaaatacga tcaagtgaga agcaaaaaca atgtgactgg
    12601 tacacaagca gcacaatata tattacaaag tcaacagata aataatgtcg gtgttcaaca
    12661 aattgccgga gatctcacag ataactacaa ttcaggtaat aagatgttga gtttgtctca
    12721 agcaactgca cagtctactt cagttgctgc gataggtgta gcagctcatg aatgcggaca
    12781 tgcggtacaa gatgcttcgg gttatgtccc attgaagatt cgtgcagcac ttgttccagc
    12841 agctaacttt ggttcgatga tttcgttccc cttgattatg gtaggtgtat tgcttagctg
    12901 gaactctacg ttaatcaata tcgggatttt cgctttttct ttagcattac ttttccaatt
    12961 ggtgacatta ccagtcgaat ttaacgcgtc tcggcgagca atccaaattt tgagtgaagg
    13021 cggactactg acggaagaag aagtaccgat ggcaagacac gtattatttg cagcagcact
    13081 gacttatgtt gcagcagcgc tttcgacatt cctgcagctg ttgcgcttga tcttattgtt
    13141 cggcggcaac cgaagagatt aaaataaaga agttgtgaaa tcagcgcttt gatctgagct
    13201 aataccgaga gttagctgat tgaataccat ttatttggaa
//
LOCUS       DAZKMB010000075        13209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000075 DAZKMB010000000
VERSION     DAZKMB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.075"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(269..341)
                     /locus_tag="ACG8V3_001923"
     tRNA            complement(269..341)
                     /locus_tag="ACG8V3_001923"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(306..308),aa:Lys,seq:ctt)
     gene            722..1291
                     /locus_tag="ACG8V3_001924"
     CDS             722..1291
                     /locus_tag="ACG8V3_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0300453.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1278..2138
                     /locus_tag="ACG8V3_001925"
     CDS             1278..2138
                     /locus_tag="ACG8V3_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0300454.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2328..3032
                     /gene="yycF"
                     /locus_tag="ACG8V3_001926"
     CDS             2328..3032
                     /gene="yycF"
                     /locus_tag="ACG8V3_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0300455.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3037..4872
                     /gene="walK"
                     /locus_tag="ACG8V3_001927"
     CDS             3037..4872
                     /gene="walK"
                     /locus_tag="ACG8V3_001927"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0300456.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4869..6185
                     /gene="yycH"
                     /locus_tag="ACG8V3_001928"
     CDS             4869..6185
                     /gene="yycH"
                     /locus_tag="ACG8V3_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0300457.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6186..7055
                     /locus_tag="ACG8V3_001929"
     CDS             6186..7055
                     /locus_tag="ACG8V3_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0300458.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7110..7919
                     /locus_tag="ACG8V3_001930"
     CDS             7110..7919
                     /locus_tag="ACG8V3_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0300459.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8022..8651)
                     /locus_tag="ACG8V3_001931"
     CDS             complement(8022..8651)
                     /locus_tag="ACG8V3_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0300460.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8821..10113
                     /locus_tag="ACG8V3_001932"
     CDS             8821..10113
                     /locus_tag="ACG8V3_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0300461.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10189..10851)
                     /locus_tag="ACG8V3_001933"
     CDS             complement(10189..10851)
                     /locus_tag="ACG8V3_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0300462.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11076..11360
                     /gene="groES"
                     /locus_tag="ACG8V3_001934"
     CDS             11076..11360
                     /gene="groES"
                     /locus_tag="ACG8V3_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0300463.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11411..13036
                     /gene="groL"
                     /locus_tag="ACG8V3_001935"
     CDS             11411..13036
                     /gene="groL"
                     /locus_tag="ACG8V3_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0300464.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4392 a         2280 c         2841 g         3696 t
ORIGIN      
        1 ccttttatag tacttagatt taatcctata tgtttagaga gttcattggc agcatgattt
       61 tgcaaagctt ctcgaattat tgttatatta gaaactttac tgttcataat tatgcttctt
      121 ttttaactca aagtccaaag ggctacagtg gactttgagt taaaaataaa aagccctagt
      181 tattttgaaa aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat
      241 ttacctgttc cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc
      301 tgattaagag tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata
      361 atagtagcac taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt
      421 tacttcctta taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat
      481 accaacaaac aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc
      541 tttcccgccg tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag
      601 ccttcttgtt ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta
      661 gattgaatgt ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat
      721 gatggaaaaa atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct
      781 gcctcactgg gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt
      841 tgatcgttat ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat
      901 ggtaaacaat tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa
      961 agaacacgta gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga
     1021 aatcaaagaa gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct
     1081 attttgcgat gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc
     1141 tcatccgcaa aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac
     1201 tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt
     1261 aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg
     1321 atgttccaga agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt
     1381 ataaagatca ggtatataca gataaaattg acattacccc tgaagagatc tatcaacgtt
     1441 tgccagagga aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg
     1501 agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga
     1561 gcgggacata taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg
     1621 tactagatac gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat
     1681 ggctggaaga aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa
     1741 gaacaaaagt tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg
     1801 gtttagttgc ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg
     1861 aagaagggat ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa
     1921 cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc
     1981 atggggatgc gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac
     2041 aagcaaacca aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg
     2101 gattattggg tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag
     2161 gtgaaaatca gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg
     2221 cactttcatt ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata
     2281 cggtataatg agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt
     2341 tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag
     2401 gctatgacgt ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag
     2461 aacctgattt gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc
     2521 gtgaggtacg caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa
     2581 ttgataaagt cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca
     2641 accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg
     2701 aaccagaaga ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag
     2761 cttacatggt gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac
     2821 tcttttactt ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg
     2881 tttggggtta tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc
     2941 gtgaaaagat cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg
     3001 gttactattt gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt
     3061 cgatcagtca attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa
     3121 attattggtg cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa
     3181 gatatgaatc aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa
     3241 gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg
     3301 actagcgata tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc
     3361 gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc
     3421 acgactaaac gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa
     3481 ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc
     3541 gaacaaaaat attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt
     3601 gcggcattta tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc
     3661 ggtgaaatga gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa
     3721 gtgcatggac aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt
     3781 attgaagaag ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc
     3841 catatgacag acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat
     3901 atggctgttt ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca
     3961 cttttagata tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg
     4021 gtacttgacc gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt
     4081 tctatgatcc gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg
     4141 acagaacaag aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa
     4201 cttcgtacac cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg
     4261 aaagacgagg aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg
     4321 attcgaatga tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt
     4381 caattagaat atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg
     4441 atggtgggaa atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt
     4501 tgggtcgaag tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg
     4561 atcaagtatt caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac
     4621 gtgattttaa gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg
     4681 ttcgagcggt tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt
     4741 ggattagcta tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc
     4801 aaggaaggaa aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa
     4861 tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt
     4921 taagcatata cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg
     4981 tagatgagac aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct
     5041 ttcttccgct tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa
     5101 atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag
     5161 tagtttcagg agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt
     5221 tggcctataa tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag
     5281 atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc
     5341 aaggaaataa aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata
     5401 tttcgttgaa ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag
     5461 aaatgacaac agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac
     5521 tgaagaagta tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt
     5581 tccagaaacc agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg
     5641 ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc
     5701 cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag
     5761 gcagttcttt agggaacctc agatattttg accgcagtga caatacgatc gattatcgga
     5821 tattcgtgga aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag
     5881 ttggcggtga aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc
     5941 aagtgccgat accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc
     6001 agttaaaagc taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat
     6061 ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat
     6121 ataaagataa ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga
     6181 attaattgga tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg
     6241 tgtttttatt cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta
     6301 atcagactga atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga
     6361 tttcgtctga gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt
     6421 cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag
     6481 acggtgatgt tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc
     6541 cagataaaat tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg
     6601 gtaatgatta tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc
     6661 aagcaagcca agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt
     6721 ctttagaaaa aaacgataat gattatagca tcacgaaata catgcagacg catttatctg
     6781 atatcgagca gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat
     6841 tgtacatcaa taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt
     6901 cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg
     6961 aaacaagcga taaagtggtc caaattgaga gcgtcaacgc tttcagcaat acgatcgtga
     7021 caaacaatac tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt
     7081 tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca
     7141 gtatcctagc aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc
     7201 ttttggtcga tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc
     7261 ggacaccgga ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg
     7321 gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg
     7381 aagcgatgga accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg
     7441 ggaaagtcag aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag
     7501 cggctccgca attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca
     7561 ccggatattg cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg
     7621 aaagtaatca tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc
     7681 gtattttagg agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg
     7741 tgatcggtaa tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga
     7801 aagaacttgc tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac
     7861 atgattttga aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa
     7921 cagttcaatc aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc
     7981 ttctattttt atttttttag catattaaaa aaataaccgt attatcgact gactacactg
     8041 atgtgctgct tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct
     8101 gccatgctga tcgtactgtc gctagcagcg tttctttgta aataatcatc acttaacaaa
     8161 taagtacctg tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa
     8221 tgtagatttt tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga
     8281 tagaagggtg catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag
     8341 aataacgaag aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca
     8401 atcaaatggg acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca
     8461 tcaacgacaa catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt
     8521 ataaacggaa catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa
     8581 tctctggaca atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt
     8641 gcaattttca taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat
     8701 tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca
     8761 ttgggtatag ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa
     8821 atgaaagagt ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga
     8881 cccttaactg ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg
     8941 ctgtttttct tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca
     9001 ggattttcta tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg
     9061 atcgtttttg ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc
     9121 ggcataacag ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat
     9181 actgtctgga caggtgctga agggatacta ggagcagtta caactgggct catcatcgga
     9241 tggggctatg cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct
     9301 gaaaatgttt ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg
     9361 cttatcggtt cagcggtttt tcaacatttg ttcagaatga ctgttgttca aggagtatgg
     9421 acatatgtcc agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta
     9481 ggtttttttg ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg
     9541 ttggttagtc ccgtcttgca agcgaattca ttagcaaatg cagaaatctt agcctcagga
     9601 caagagttga cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc
     9661 atgacggtta cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg
     9721 aaatcgaaga aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc
     9781 aatgaatcaa ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt
     9841 attttcgcac cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt
     9901 cctttattta cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta
     9961 gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt
    10021 tatttcccat ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa
    10081 aatgatggag cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag
    10141 attgggaaaa tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc
    10201 tttttggaca gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat
    10261 cgatgtccag atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa
    10321 gtagattagc agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc
    10381 tccgacccaa aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa
    10441 ctcttccatg atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat
    10501 catctggatg atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc
    10561 taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat
    10621 aacaatcttt ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg
    10681 cttaaaataa aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt
    10741 gatcaattca gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa
    10801 aattagacca tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct
    10861 ccttttaagg gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca
    10921 taatttcaat aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa
    10981 aaagagggag aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta
    11041 acagattaaa aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg
    11101 tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg
    11161 ctgctaaaga gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag
    11221 aaaacggtga aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat
    11281 acgccggtac agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata
    11341 tcatggcgat tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg
    11401 tgaatcaatc atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg
    11461 cggggtagat aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt
    11521 cgtactagaa aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa
    11581 agaaatcgaa ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc
    11641 atcaaaaaca aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc
    11701 aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg
    11761 cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt
    11821 tgattcaaaa gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg
    11881 acatttgatt gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga
    11941 atcaaaaggg atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta
    12001 cttatcacaa tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata
    12061 tatcttgatc acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca
    12121 aatcttgcaa caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt
    12181 gccaactctt gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc
    12241 tggtttcggc gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac
    12301 agtaattact gacgatctag gtttagaatt gaaagatgca acaatcgaaa acttaggaaa
    12361 tgcttctaaa gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa
    12421 agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga
    12481 ttttgaccgc gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt
    12541 taaagttggt gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc
    12601 attgaatgct acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct
    12661 agtgaacgtt atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat
    12721 caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta
    12781 tgaaggatca gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc
    12841 tgctactgga gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac
    12901 acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt
    12961 cgttgcagat aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat
    13021 gggcggtatg atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac
    13081 agataaaaag aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga
    13141 aattgctttt caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc
    13201 gctgtttat
//
LOCUS       DAZKMB010000076        13141 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000076 DAZKMB010000000
VERSION     DAZKMB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.076"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..781)
                     /locus_tag="ACG8V3_001936"
     CDS             complement(<1..781)
                     /locus_tag="ACG8V3_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0300465.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYN"
     gene            complement(778..1431)
                     /locus_tag="ACG8V3_001937"
     CDS             complement(778..1431)
                     /locus_tag="ACG8V3_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0300466.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1719..2239
                     /locus_tag="ACG8V3_001938"
                     /pseudo
     CDS             1719..2239
                     /locus_tag="ACG8V3_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2237..2956)
                     /locus_tag="ACG8V3_001939"
                     /pseudo
     CDS             complement(2237..2956)
                     /locus_tag="ACG8V3_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2907..3116)
                     /locus_tag="ACG8V3_001940"
     CDS             complement(2907..3116)
                     /locus_tag="ACG8V3_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300467.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(3098..3166)
                     /locus_tag="ACG8V3_001941"
                     /pseudo
     CDS             complement(3098..3166)
                     /locus_tag="ACG8V3_001941"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            3393..4666
                     /locus_tag="ACG8V3_001942"
                     /pseudo
     CDS             3393..4666
                     /locus_tag="ACG8V3_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4773..5804)
                     /locus_tag="ACG8V3_001943"
     CDS             complement(4773..5804)
                     /locus_tag="ACG8V3_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300468.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5811..6641)
                     /locus_tag="ACG8V3_001944"
     CDS             complement(5811..6641)
                     /locus_tag="ACG8V3_001944"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0300469.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6638..7453)
                     /locus_tag="ACG8V3_001945"
     CDS             complement(6638..7453)
                     /locus_tag="ACG8V3_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0300470.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7450..8274)
                     /locus_tag="ACG8V3_001946"
     CDS             complement(7450..8274)
                     /locus_tag="ACG8V3_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0300471.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8264..9364)
                     /locus_tag="ACG8V3_001947"
     CDS             complement(8264..9364)
                     /locus_tag="ACG8V3_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0300472.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9542..10327
                     /locus_tag="ACG8V3_001948"
     CDS             9542..10327
                     /locus_tag="ACG8V3_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0300473.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10360..10743
                     /locus_tag="ACG8V3_001949"
     CDS             10360..10743
                     /locus_tag="ACG8V3_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0300474.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10819..10950
                     /locus_tag="ACG8V3_001950"
     CDS             10819..10950
                     /locus_tag="ACG8V3_001950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0300475.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10919..11155
                     /locus_tag="ACG8V3_001951"
     CDS             10919..11155
                     /locus_tag="ACG8V3_001951"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0300476.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            11167..11253
                     /locus_tag="ACG8V3_001952"
                     /pseudo
     CDS             11167..11253
                     /locus_tag="ACG8V3_001952"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(11233..12204)
                     /locus_tag="ACG8V3_001953"
     CDS             complement(11233..12204)
                     /locus_tag="ACG8V3_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0300477.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            12856..>13141
                     /locus_tag="ACG8V3_001954"
     CDS             12856..>13141
                     /locus_tag="ACG8V3_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0300478.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         3955 a         2898 c         2348 g         3940 t
ORIGIN      
        1 tattgtattt catgttttcc agtattattt taagactagc aagaggggtt ttaagttcat
       61 gggatgcacc tttaaaaaag tcatatttta acttttctaa ttttaaaatt tctttatttt
      121 taaattccaa atcgtcaatt gttcttaata aagtagaata caaatcatta atttgttgtt
      181 ttaattctcc aacttcatca ttagagctaa cttttaaact cattttttta tcaagtttca
      241 tcattttatc agtaacaatt tttatttctt gtatattatt ttttattgat tttgcataaa
      301 ttaaagaaat aatagcagaa aataaaattg atattaataa agaatatggt aaaaatttaa
      361 ggcttaaatc tttcgcatct ttttgcatat cagctgtaga aacaaattgt agatttgttt
      421 ttttaccatc attaagcttt atttctcttt cttcaattat caaagaatta ctatcacttt
      481 ctaaattaac atttatatta tctttgattt gtatttcatt cttattattt ttttctttta
      541 tgaatacttt tatttcacta ctcttggaat aaaactccaa agtttgttct atatatttta
      601 tttcttttcc attcatatta ctagaaattt catttgccat attatgaatc ttttcttttc
      661 tcgtctccaa atatgtttta ggaaagataa aaaaaattaa tgaatgaact aatataatta
      721 ttattccaag aacagaaaat atttgtatga acatttttgg aaatatcttt aattttttca
      781 ttttctctcc aatttatatc ctacatttct tatagtagtt atacagtcta attgtaattt
      841 ttttctaagt tcttttatat atacatctat tactcgatca taaggagttt cttcgctatc
      901 tttccataca taatcaataa tttgcattct tgttaacact tgtccattat tatccaataa
      961 acattttaat acttctagtt cttttgcatt tacatctatt tcttcaccat ttattttagc
     1021 tgtatagcta ttaaaattaa ccgatagatc cttatattca aacttctcta aatgtccata
     1081 attcttctta attaaagaat ctattctagc ttttaagacc ggcaatgaaa atggcttttc
     1141 tacatatccg tctactaaat tagtaaatgc atcaatttta tattcttcat cactaaatgc
     1201 agtcaagatt agaattggta aattgctttt ctttctaatt tctttcaaca cttctaaacc
     1261 atttataaaa ggtatctgaa tgtctaagat aaccaaattt atatcgctat taaattttga
     1321 caaggcttct cttccatctt tagcttgaat aacagtatat ccaaattctg aaagatattc
     1381 acttattccc tctcttatca tattatcatc ttcaacagtt aatattttca tatatacctc
     1441 cacttaagcc tatacactag atattataca gcatattatg gctctataat atctagtgtt
     1501 ggtgacgcct tctgttgcca atactttttc ttttttttaa taaatgcaaa cagacacctt
     1561 gaaatccttt agaaaaaagg cttctataat attttgtgtt gttggaaaat cgaaggcgat
     1621 tttctactaa cactttttct tcgtttcaac tcaaaaagag actcactttt ggtaaaattt
     1681 agtcgaccaa aacatcaccc taccaaagga gtcgctctat ggataatcat actagaaaat
     1741 tactcggatt aacagataaa catttatttt ttgatgaaga atggctgatt gaaaaagaat
     1801 ataaaggcgt tcaagcgcag tttattaaag ggagattgga tgactcacct tcccattgtg
     1861 aacactgtgg atcgattcgt attatccgca acggttccta tacgacacgt actcagatgt
     1921 tgaaagtcaa agaaaagttg actattttag aattaaaacg tacccgtttt ctatgctatg
     1981 attgtgggca gaccttttct gcgaaaacgg acttagttga tgaacatcat caactaacaa
     2041 aggaactgaa gcaggcgatt ctcatagaac tgtacgaaaa tcaatcacgt aatctgaaaa
     2101 tcagaagaag taccgtcggc tcaagcgata ttggaaactc ttactcaaag attccacgac
     2161 attggagccg ctcaaacggc attatcatcg actgtttaaa cgaccaatta gtcaaacaga
     2221 aatcgtcgat gaaccgttgt gcacaatggt gtcctgaacg tacagcgaca ccttccatat
     2281 ccagagcagt cgccacatca cggggatgga tgccatccaa attaaaagca accacgccag
     2341 tatgatgttc aggatcttgc ggtccgtaaa tggtgatacc gccgatcgct tgtaatttag
     2401 gtaaaacgta ggcaaccagt tcttgctcat aacgatgaat attctccata ccaatctcat
     2461 tcaagtaatc gattgctcct gcaatgttcg gagtaccagc ttcaaatttc catggcagct
     2521 ctttccatga actatcgtaa agattgacaa aatctatcat ttcaccgccg aattcgactg
     2581 gctccatttg ttctaacaat tgacgtttgc catataatac accgattcct gtcggcccgc
     2641 acattttatg cccgctaaag gcgaaaaaat ctgcatccaa cgcttgtaca tcaactgtca
     2701 tatgcggtgc tgcttgggca ccgactggac tatcctcaac tacaacagcc tgttcaaccc
     2761 tcagccgttt caagcacaac aaataacctt ctggatctgg tttgccgacc tgaagatcat
     2821 cgatcgtcac aaaggaatca attttttcct taatcatcgg atgtgcttca aaaaaatagt
     2881 ctagctcccg cagttcagaa ttgagactac tccgattttc cagtgctgct gatccgctgc
     2941 tcaaaatgtt cagcatccac tggtctccca accgcttgac ttatcccttc ataaaaggac
     3001 agttctgctg ccgtcccaac accttgttga tagtctgcaa aaggaatttt atagccgaac
     3061 tgctccagta aataggcttc agtaaatagt gatgaatact ctcgtaaaac atccattcac
     3121 tgtcaatcat cgtcccatca aaatcaaaaa taagtgcttt ggtcatcttc tttccccctt
     3181 acccaaaaca agcaaaagct gtgtcctgct ctctctacag tatcagcata aaaactgccg
     3241 tctgaaaagt caaaggtgaa agaaatgtgg cgctataatt tctaggactg atagtaaatc
     3301 attctatcag tccttttgag cataaaaaaa caaaacatac agaagctccg ttagaattga
     3361 atcaccacaa cacaatcaaa ggagcgttct gtatgcctga tcttcatctt atcaaactat
     3421 tactaggaat caaagatgga aacattcaaa tcactcatgt cgagaagaaa aaaatacatt
     3481 caatcaacag tatcgttgtt tctgccacgc tcattaaaac gattcagccc tgtcctcagt
     3541 gtaaacaggt gaatcaacat gggctgatcg ttaagaacgg aaagaaaaaa tcgctggtcc
     3601 ttctcaataa gtgtgcgaat caacggactt atcttgcttt ggcaaagcaa cggtacctct
     3661 gtcgtggctg tcacacctat tttacggcga atacctacat tgttgaaaaa aactgtttta
     3721 tcgccaaaca agtacattat aaaatattgg aggaattgac tgaaaaacaa gcaatgacga
     3781 cgatttctaa acattgcggc gtttcttggt caaccgtttc gcgaacactc gcttctctgc
     3841 tccccatgac aaacgtgaaa ataaattggt tgcctcgctg ttcattggta gatgaatttc
     3901 gttcattgaa aaatcaagtc ggtccctatt ctttttcttg tatggatggc gatactggga
     3961 aattactgga tattcttcct tcacgtaaga agaaagatct catttcttat tttatgcaat
     4021 ttgaacgacg tgcacgttta aacgtaaaaa tactcgtaac agatatgaac gcctcttatg
     4081 ctttgctgat aaaagagtgc tttcctcatg ccaaactcgt tgtggatcgc tttcacatcg
     4141 tcaagcacct cattcgcagt tttaaggata tttgactatg agtcatgaaa agcttcgacc
     4201 gaaacgatcc aatacaagcc aaacactatc gacaagtgaa ggctttatct cgacttctga
     4261 tcaaacgaca ggacacgctt gtttatgata aatggacaaa atggcgtaat tttggttggg
     4321 cctatttaac cgaaagtgaa gtcgtagaac gtctactttc tacttctgat gaactacgaa
     4381 tagcttatgc ttactatcaa gaaattcttc aagcttttta tgacaaagaa gcggacatct
     4441 tctttcagct tgtaaaaaca atgcccaagt cggttccaag agaactgcat catataaaaa
     4501 aagcgtttat aaactatgaa tccggcattc gtttagcctt agagttgcct tactcaaatg
     4561 gcaaaataga aaatctgcat acccacatca aagcattgaa acgaatagct tacgggttag
     4621 gatgagaacc agaattttct taatcaatca tctgatcact tattaaacat aaaaaaacat
     4681 ctaaagcaga aaatcaattc taccttagat gttcttatat cactctatca gtcctatttg
     4741 acaaagagcc agaaatgttt gccgactata cactagcttt gttgtgcaaa atgctgagta
     4801 tcaatttcca cagggattgc tagaatatct ttcagctgaa tgtataaggc atcgctaatt
     4861 ttctcttccc gatcgttcaa ccgatccact aaaatttctt tgccctccag cgtttgaaaa
     4921 acataccgtt gaatgttgcc taaaataaat gattccttcc atagaaccgg aatcttaacc
     4981 ccctgaggaa ccggttcaaa ttgaatcagc tccggtctta aataataaat gcagcgattg
     5041 cccttagcgg aaaactcgtg ggtatactga ttcagctcat accattcgaa acgattatag
     5101 ttgccgataa attccgcaac aaatcggctc tgaggattct gatacaattc cttcgaggtg
     5161 gaaacctgag ccacccgccc agcttccatc acaaaaatcc gatcactgat tcgcatcgcc
     5221 tcttcttggt catgagtcac aaaaatcatc gtcatcccta gcgattgctg gatagcgcgc
     5281 aaatcccgct gcagctgttt tctaattcgg gcatccaaag cacttaatgg ctcatccagc
     5341 agcaggactt tcggttcagt gaccaatgag cgagccagtg ccacccgttg tcgttgaccg
     5401 ccagaaagaa actggacatt tttatctgcc tgctccgtca tatccaccaa tgatagcatc
     5461 tcaaaaacct tttcctgaat cacctcagga ctcagcttct ttaccttcaa accaaaggct
     5521 acattttcaa aaacagtcat agttggaaac aatgcatact gttgaaaaac aaagccgata
     5581 ttgcgatcct tcgtaggctt atcggcaata ttctcattgt ccagcacaat ctcaccgctc
     5641 tttggttgtt ccaatcccgt gatacagcgc agcaacgtag actttccgca cccgctggga
     5701 cctagtaaag taaccagctc acccttttca atctgaattt catcaatagc taaaatctgt
     5761 ttttcatcaa aaaacagatt gagatcatgt atttctaaaa acattttccg tcacaactcc
     5821 tttaagggct tctttttcga ctgcttctct tccctcgtct gcttaaggga cagctgttgc
     5881 ttttgtcgat aagtcagcat aaaggtagtt aaagcaattc ccaccataaa cagcaaatac
     5941 aaaaccatca cagcgctagc caaatggccg ttttcgttca tccgccgcag catatagatg
     6001 cgtaccgtct gaaatcgacc gccaatcaac aggttcgtca gcatgtactc cccaaaaacc
     6061 gaagaaaaaa tcatcagaga aactagggtc acaccgatac ggatgtttgg aaacagcacc
     6121 tggataataa tcgtagacat cggggcgccc aaggtttctg ctgcatccac taattccttc
     6181 agattaatta agcgcatctg gttgttaaga ctaagataga caatcggcaa cgccgtaata
     6241 aacaatgcac ctaccaacac tacaaacatt gggataccag tttcagcata cgttcgaagc
     6301 aaagccgtta ccaaaatcac tcctggtaaa gcatacggca gcaggagcag cttttccagc
     6361 caacgattca aacgagggaa atacaaatga atccaaataa tggtgggaat catcaacaac
     6421 agaatcgtca ccaagaccac acctgctaat aaaagtgagc gacccaccgc agcaataaat
     6481 tccctgtccg tcagcagctg ttggaaccat tgcaaggtaa aatcactagg taaaagcgat
     6541 tttgaccatt tagtggagct ggcatacaaa atcgttccaa taatcggcag caagtaatac
     6601 aggacaaagc agaacaaaat cggttttatt aactttttca cgtctgcacc accttgcctt
     6661 tcttttgaat aatcaacatt gccgttacca gcaggacacc taaagcgact gccaacgcac
     6721 tgcctaaatt aggcttggcg aacacatctc ccgacactaa agccgcaatt cgaatcgtta
     6781 aaagatttac attactgctg gtcagggcat acgctgtttc ataggtcccc atgccattag
     6841 caaataagat gacaaaggtg ctggcaatcg atggtcgcaa aaaaggcaaa gccaccttga
     6901 accaagcata cgctgatgaa gcgcccagca attgtgcgga ctccagccat tcctttttca
     6961 cttccttcat gctgggatac aaaaacaaga cccctaacgg aatttggaaa taggtatacg
     7021 tgatggtcaa tccccaccaa gaatacaaat tgaagtccgc tagaaatggc aaaatcattt
     7081 tcagcacacc tacattcccc aacaaaataa taaaagagaa tgccagcgga atcccagcaa
     7141 aattagctgc caaattggaa acaaaggtga ttttctcctg caatttagtc gatagattca
     7201 ttaagcataa acataaaata aacgtcccga ttaacccaat cacactggag ctgacagcaa
     7261 tcagtacgct gttaacaaag gcctgtgtgt aatatttgtt gctgaaaata tcactgtaat
     7321 tggccaatgt ccactggtta gttaaatcac tgtgcaagct gcacagaacc ataaaaagca
     7381 gcggcagcag taaaaatgca cagaagataa aggaggcagg aaccagaacc aagcgttttt
     7441 tcatctcttt catcccctca tcaattcttg aatgtcctgc attttctctg acggtgcaat
     7501 tcctaataac cagcaaaaca atggagccgc cagcagctgt ggtacagttt gttccttcag
     7561 cttttggcgt ttatttgaaa tcaaataaaa aggaacttca cgatgtgcag ccaagctgcc
     7621 gccatgcagt ccgtgcgcat ccatcccgtg atccgcagtc accacgactt gataaccttt
     7681 ttgcagccat tcaggaaggt aaatagccaa taatctgtcc gcctgattaa ccgctgctgc
     7741 atattcatga gaatcggcgg taaatttgtg accgacatca tcaatattca tcgaatgtac
     7801 gaccaaaaag tctggctgat actgattcac taaataatgg gcatccgcaa ataaatgcga
     7861 atcagggtaa gtgtcctcac tataaaagat gccatgctga atcagtcgct cctcctgatg
     7921 ctggatgcga tcaaccaaac gattataggg ggcttgattg tacaactcgc tgtaccagtg
     7981 gtaagcggcg gctcctgttt ttccgccagc ttccttaact aattcaaaca gcgaccactc
     8041 tttggatcgc tgagcatggt aattactata gatgccattt tcataggctg gaacgccggt
     8101 catcagtact tcgtataacg gacgtgaatt gctgggcagc tcacttttta ccttcaataa
     8161 gcttgcttgc tggtgctcaa ctaaatgatt catgaaccca agctgggcga cggctgtgtc
     8221 gtagcgacag ccgtctagta taactacaat cagtttgtca agtctacttt gcataggtca
     8281 gtacctccgc ctgccaaagc tctggaagct ctacagaggt cttgttccaa gcttcattgt
     8341 cttcaactgg caccgccgct tcataagcat cctccggcag taatttttca gctacttctt
     8401 taggcagctc taccttgcga ataggtcgcg catagccctt tgccaaattg atttgccctt
     8461 catcagataa aatatattcc cgtgctagct ttgccgcatt gggatgaggg gcatttttat
     8521 tgattaaggt tgtatacccg ctaatgatgg agccgtctgt tggcacagtt acctcaaaac
     8581 gctcggagtc aatttggtca cgataattta gtccattgaa gtcccaaaca actgccacat
     8641 cgatttcacc tttttcaaga tttgctacgc tggcatcaac ggtggacaaa cggccttttt
     8701 ctgccatttt tttgtagtaa ttcagacctg gctgaatatc cttttcgtca cctccgttgg
     8761 caatcgccgc agccaataca gcaaattgtg cttggttggc aatcgtcaca tcccccacgc
     8821 taacttgata gtcaccctct tgcagctcct tccagctagt cggtgcttgc ttcacattct
     8881 ctttatcggt gataaaggaa atggttcccg tatagcttaa caaccaatcc ccatcatcat
     8941 cctttgccca atccggaatt tcctcccaat agttggtctt ataaggtaag gttaactctt
     9001 tgctttcagc caacggaccg aagctgatac cgacatcacc aatatcggcg gttccatttt
     9061 ttccttctga ttcaaactta gccaattctt ctgcactgga catatccgta tccacgtgac
     9121 ttaagccgta ttctttttca aggtcgttcc aagtgcctac ccaatttgcc caagtatccg
     9181 gcattcccaa gctggcaatc tctccttctt ccttggcttt ttctataatc gtatctaaag
     9241 agtcgtctgc cgcagccgat tgcttcgttg cttcacctga gccgcagcct gtcagcagga
     9301 tacttactgt tgctgcacaa aataaaccga tagtcatgtt acgaattttt tcattcattt
     9361 tcattcattc ttctcccctt tcgataattc cattctaaca aagctttgta atgagagaat
     9421 cagtttcttg aaaaggtttc atgaacaatg taaaggttgt atatggtttg taaatttcga
     9481 cgaattcccg ttaaccttct aaagcttttg cggtatgata gtagaaaaaa ggaggctccc
     9541 tatgcagtat tatgatcagc atctccacac gtatttttcc tttgattcag aggaaaaatt
     9601 tgaaaactat ttagcctatc agccggagtt tttcgtttct acggatcact ttgatttaaa
     9661 caatccatct accggaaaag atgacattcc ggattatcca gcctatataa acaaactgga
     9721 ctctttaaaa gaaacctatt ccaccaaatt tctgcgaggt atcgagattg gcgttgttcc
     9781 aggacaagaa gagcggatcg tggattacct cgctcagcat ccttatgatt taaagctggt
     9841 aagtattcat caaaatggcc gctttgatta tatggacgat gtggttttaa ccaaagatcc
     9901 cattcaaact accaaggaat attttgatca gatgttgcag gtcttgacgg attttccaga
     9961 aggagatatt ttgactcact ttgattatgg attacgccgc ttcaccttca cagcggaaga
    10021 attcgcggag cattttgagg ctcagctgct aacgattttc aaaaaggttg ttgaacgaga
    10081 attggcgatg gagttaaatg ccaagagctt cttgaaatac ggcaacgaag acctttaccg
    10141 ttatgccgtt cctttgtatc aaagtgtcgg cggaaagctc tttaccttag gctcggatgc
    10201 tcatgttgcc gaagattatc agctgggctt taaaaaaatg agtcagctgt tggcagactg
    10261 tcaggtagaa aagttgctag tcattcaagg aaaagaacgc cgcctgacgc cgctgccgat
    10321 tttgtgattc ctatataata gaagaaagtg gtgaaaaaaa tgagcaacct gtgggactac
    10381 aatcaagagg cacccatcca ttatttaatt gcgcgacatt gggatgcgtt gaaaattgag
    10441 gcggtttgcc gatccttgtt agcagccgtt ccaaaacaac aactggaaaa ttttttggtg
    10501 gctgacagct tgcagcggga aaaagttcag gcttattttg cagcctttaa agatcagcct
    10561 ttggaatatt tgcatgcgca gtttcatctt ttttatcaag tagccgcccc agatgactac
    10621 aacgatttac gaggacagct gcaattgacc tttcaagcgg atgaaaccgc ctataccgtt
    10681 ttattgggaa tggctcgcct tggtgatcag gcgaaggtcg agtggcggat tttcgatatt
    10741 tgatggcagg atcaaggcgt ttcagttgca aaccttcctc tctttcaatt agaaaaaccg
    10801 cagcaggaag aagtttgtgt gctggtcacc gatgctggtg aaattcgtct ccgcttattt
    10861 cctgaccaag ctcccctggc tgtagccaac tggcgctccc tagctgagca aggcgtttat
    10921 gacaacacac ctttcggctc gtgtcattaa ggaatttgtt attcaaggtg gcgctttgga
    10981 tggttctggt gatgaagctg tttccagcta tgagggcttt ttcgctgatg aagtgcatcg
    11041 cggactttat cattttaatg gcgccttggc cttaggggac cacggccccc acaccaacgg
    11101 caaccagttt tttattgtgc aaaacacaaa ggcgcaggct gacctgctaa tgtaaagcaa
    11161 gcatacttgg agcttggagg actaccggag ctggatggtc gttatactgt ttttggacag
    11221 gtataccaag ggttagaaat cgtagcagcg atcgcagctc agccgacaaa cagtgaagat
    11281 gtaccgattg agccagtgtt cattcgacaa attaacttcg aaaaaacatc gtaaaaagac
    11341 actcactgac ttcagcaaaa gctctattct tgagctggct gcagctagtg agtgtctttc
    11401 atttatccat aagataaagc ttggattcaa taaataaata aatgttttgg ttaaagctgt
    11461 gaccagcaaa tgaggaaccg tgcgagccag ttgatctgcc aatgcaaaca tttctttagg
    11521 tgaaggcgct ggcgtgtctg ccaacggata ctccagtccc aatgcttggt aaccgtgcat
    11581 accatagcag tggtacggta gtaattctaa cggtacgaac cgatcaagat ctgccacaaa
    11641 acaagcaatc gtcagcagct cggtcaggtt catattgata cctggaacca caggaactcg
    11701 aattcgcact tcgcagttag gatactcaat taaaaagcga cgaatattct tcaaaatttg
    11761 ttggttatct aagccggtcc attgtttgtg gagcttgctg gtaaacattt ttacatccac
    11821 aaacaatgta tccacatagg gagctacctt ttggatttca ttataagcac cacaaaagct
    11881 ggtttccagt acggtattga ttccaatgta tttcgatttt tgcaaaattt ctttagcaaa
    11941 atccgcctga accagtgcct cgcctccgct aatggtcact ccgccatccg agtgaaagta
    12001 aaaaacagca tccttctcaa tctcatccat tacctcttca gcggtcatgg tctggccaaa
    12061 tcctttttca atctgcttgc tttgtgattc gggaatcgaa caccaggcac agcgtaatgg
    12121 gcagcccttt aaataaacaa cggtccgtaa accagcacca tcatgtagag aaccctgttc
    12181 tattcttaat actgttcctt ccatatagat ttttgaatta cacactagtt taaactaaaa
    12241 cacacaagga aaaacttacc atttaatcat aaaattagct catataaata tactaggatg
    12301 taaaatagac tggtcaaaat ctggtcaaaa aagaggacta atacaggtag tcctcttttt
    12361 tgttatttta atattctaaa tcatttttta ttggtttatt cccttattcg atgtagactc
    12421 aaatcactat atcactagat atcaaacggc gtcatacgga atcaaaaaca tcaaatttga
    12481 cctgttaaat ggtcattgaa taacattcaa aatcaataaa acggagtcag acacggagtc
    12541 aaaaacgctg agagtaaaaa atatatacag ccttgtaggt aaagtaccac agggctgtat
    12601 attttttatt tcagattacc ttaaatgatt ccatttactt tttatctcat tttttcctac
    12661 tagttagtat caaatactct cctattttct caataaacta tttttggata catggtatct
    12721 gaaacagcta aaaccttgtc attacagcaa aaaatcacgt tatccagact gtagacaaag
    12781 tccattttat ggatgatgtc tacagtcttt tcttttataa tagatttatg aaaacgaatg
    12841 caaggagtga tagctatgat gtctaaaaac aatacgaatg gtagaaatca atttgcgatg
    12901 cttacgattg atgatctagt tcctcaagat catctcgttc gaaagattga tgccgcgctt
    12961 gattttgaat ttatttatcc aatcgtcgaa gccacttact ccgatttagg tcgcccaagt
    13021 atcgatccag tgattctgat caaattggtg tttattcagt atttattcgg tatccgttcc
    13081 atgcgacaaa ccattaagga agtcgatacc aatgtcgctt accgctggtt tttaggctac
    13141 t
//
LOCUS       DAZKMB010000077        13073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000077 DAZKMB010000000
VERSION     DAZKMB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.077"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      198..364
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            512..1069
                     /locus_tag="ACG8V3_001955"
                     /pseudo
     CDS             512..1069
                     /locus_tag="ACG8V3_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1127..3169
                     /locus_tag="ACG8V3_001956"
     CDS             1127..3169
                     /locus_tag="ACG8V3_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0300479.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADDKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAE
                     IKEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3278..4429)
                     /locus_tag="ACG8V3_001957"
     CDS             complement(3278..4429)
                     /locus_tag="ACG8V3_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0300480.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4583..5113)
                     /locus_tag="ACG8V3_001958"
     CDS             complement(4583..5113)
                     /locus_tag="ACG8V3_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0300481.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5212..5562)
                     /locus_tag="ACG8V3_001959"
     CDS             complement(5212..5562)
                     /locus_tag="ACG8V3_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0300482.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5719..7248)
                     /gene="ahpF"
                     /locus_tag="ACG8V3_001960"
     CDS             complement(5719..7248)
                     /gene="ahpF"
                     /locus_tag="ACG8V3_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0300483.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7258..7821)
                     /gene="ahpC"
                     /locus_tag="ACG8V3_001961"
     CDS             complement(7258..7821)
                     /gene="ahpC"
                     /locus_tag="ACG8V3_001961"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0300484.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7965..8627)
                     /locus_tag="ACG8V3_001962"
     CDS             complement(7965..8627)
                     /locus_tag="ACG8V3_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0300485.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8811..10457
                     /locus_tag="ACG8V3_001963"
     CDS             8811..10457
                     /locus_tag="ACG8V3_001963"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0300486.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10448..11530
                     /gene="uxuA"
                     /locus_tag="ACG8V3_001964"
     CDS             10448..11530
                     /gene="uxuA"
                     /locus_tag="ACG8V3_001964"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0300487.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11638..13073)
                     /locus_tag="ACG8V3_001965"
                     /pseudo
     CDS             complement(11638..13073)
                     /locus_tag="ACG8V3_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         4380 a         2394 c         2425 g         3874 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca
      541 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
      601 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
      661 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
      721 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
      781 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
      841 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
      901 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
      961 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
     1021 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
     1081 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
     1141 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
     1201 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
     1261 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
     1321 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
     1381 taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
     1441 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
     1501 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
     1561 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
     1621 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
     1681 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
     1741 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
     1801 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
     1861 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
     1921 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
     1981 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
     2041 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
     2101 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
     2161 gacaatcgat gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc
     2221 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
     2281 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
     2341 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
     2401 aatgattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
     2461 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt
     2521 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     2581 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     2641 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     2701 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     2761 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
     2821 ctatcctaaa ttgattgcag atgataaaga gacaaaagat aagaagaatg tcatcagcga
     2881 aacagcagta caaacgatcg tgccagatct gagagaagtt gtgcaagatg taaatggtac
     2941 agcacattct ctttctgctt tagggattcc gttggcagcg aaaactggta cagcggaaat
     3001 caaagaaaaa caggatgtaa aggggcaaga gaacagtttc ttgtttgctt tcaatcttga
     3061 taaccaaggt tatatgatgg tcagcatgtt ggaaaataaa gaagatgatg attcagcaac
     3121 caaacgagca tcggaactat tgcaatacct caaccaaaat tatcaataaa aaattgcttt
     3181 ttaagtacag taaaaggact gtggcgttat cgtcacagtc cttttttcaa ataaacggtt
     3241 ttcacgatgt ctctttcaca acttcttttt gatttactca cacttcataa actttttttg
     3301 taaaatattt gagaacaagt ggagaaataa gtgtacttaa taaaatcacg ataactaaag
     3361 gtgaatagta ctgtggttcg atcaattgac tttgctgtcc aatttgtaaa atgatcaaag
     3421 ccatttctcc acgcgaaatc ataccagcac cgaccatcaa tgaactatgt tgagaaaaac
     3481 cggcaatctg agcaccaaaa taacctccaa gcaatttggt caaaatcgct acaagcgtca
     3541 atatagaaat aaagagtagt tgttcactaa agtcagaaaa atcaacttct aatcctacac
     3601 tgacaaaaaa tactggtata aatactgcat atcctaaagc ctctacattg tggtatactt
     3661 cttctttaac ctttgtctga ctgacagcta tcccagcaaa aaaagcacca atgacagagc
     3721 taagcccaac aagatccgct acgtaagaca ttcctaaaca aataactagc gatacgatga
     3781 taatagaaga atttgcgtac actttttctg caaatgaaag taggaaaggc gcaatccatt
     3841 tgactaataa gaaaataaaa acaaaataaa ttagctgttc aatcaaaaca agcggtaaag
     3901 aagcattgct agaagcttcc cccgttagaa aagataggct gaaacttaga actaaaacga
     3961 ctaaaatgtc atctaccaca gaagcaccca aaattgttga tccttctttt gtattcacta
     4021 cgtttagttc ttttaatact tctactgaaa tactgacaga agttgccgca agaataatcc
     4081 caaagaaaat cgcttcattt cccgaaactt ggaatgcttt gcctgtcaac cagccaaaaa
     4141 caaccgggaa gatgatccca agcaaagcaa cgaacatacc aggtcggaaa tattttttca
     4201 aaagagataa atcactttcc agaccagcta aaaacattaa taagatcaca ccaatctccg
     4261 aaaagtcatg gactagaata tcaggtttga cccaattcaa tccagcttgg cctagcaaaa
     4321 caccaacaag taattgtcca atcactgctg ggatacctaa tctccgcgag atatgtgagc
     4381 caatcgttgt tgaaatcaat atcaggcata aaataccaat aaattccatt ctcatcattc
     4441 ctttgtataa aatatagccc gaacttagca taacatacat tattgaaaaa ataggaaaat
     4501 atactcacaa aaaaaccaac aatccaaaag atttttttgg attgttggtt tttatacggt
     4561 ctattttaat aactaaaaat gattaacgta tagtcatttt tcttattctg atgaaattga
     4621 taatctcctg gatgcccatg aaaatcaaaa taaagccgaa tatttggaat agaagcatca
     4681 aacttttgaa tggattgaac aataagacga atccgccaat caacaacaga atactactat
     4741 aaacactcca acttttagct tttgtattca tgctgaagaa caactggaac aagctgttga
     4801 tgataacagc taatccaaga agaatcggca ggatagaaac gattgcaggt gcaaaagcat
     4861 agacgatcag agctaagatc aggaaaaaaa gaccactgaa cagaccgata ctccaagaac
     4921 ctgttttcct tttaaaacgg taagcctcaa aaagattcaa caacccaagc aaaacaagat
     4981 aggcggtgat cagataaccg ataaaatgaa aaacagcaga agggtttagc acaatcgcaa
     5041 tcccagaaat aatataaaca gcagctctta ataaggcgta tttttgaata ttttgtttaa
     5101 tcgtctcaaa catataagaa aacctccttt gacttatttt aacgtgaact agtcatttta
     5161 ccaaattaaa tgttacaaaa tgagcgaagg agaagaatat ctctggaaaa atcaacttct
     5221 gaggatcttt tccatttttt tgccttttgc tagctcatcg atcattttat ctaaataacg
     5281 aatttctttc atcaagttat cctcgatgtc ttctactcgt acaccacaaa caacacctgt
     5341 aatcaaggaa cgtgaaggat tggggacagg ggcttccgca aaaaattgtt cgaaagtcac
     5401 ttgtttttgt agttgatatt ctagttctgt ttgggaataa cccgtcagcc aacaaatgat
     5461 ttcatctact tcctctttta tacgatcttt tctctccgct tttgctacat acataggata
     5521 aacgcttgca aaacttgtcg taaaaatacg attttgcgcc ataatcagtc acctttcttt
     5581 ttttcaaatt atttactact tatagtatat aagaaaaaga gaagaaaaag cgattattca
     5641 aaaaaacaaa aaggattgtg acaaaattct tgtcaccatc cttttttccg ctctctgact
     5701 ttttacaatc ttcatagctc aagtgcgcat cagataatcg aatgctccta aagctgcagt
     5761 agctcctgaa cccattgaaa caatgatttg tttgtatgga ctatctgtac aatcaccagc
     5821 tgcgaaaacg ccggggatat tcgttgcgcc atttttctcg gtcactattt catttcttgg
     5881 attcatttcg atcgttctat ctagccaatc cgtattaggg agtaagccga ctagaatgaa
     5941 tactcctgaa acagctagtg tatgcatctc catcgtacgg cgatcgatat aggagagaga
     6001 ttctacctga ttcgtaccgc tgatttcttt cgtcgctgca tgtttgataa cagtgacatt
     6061 cgcaagagag actagtttat cctgtaagat ttgatctgct tttaattctg gtaagaattc
     6121 tagaacatat acatgttttg ctaacccagc caaatcgatt gctgcttcaa caccagaatt
     6181 accaccaccg ataacagcaa tctcttttcc tttaaataaa gggccgtcac agtgtgggca
     6241 ataagcaatt cctttatttt taaactcttt ttcgcctggt acattgatag aacgccagtg
     6301 cgcacctgtc gcgatgatgg cggtttttgc tttaagtgat ataccgttag ctaagccaat
     6361 attaataaaa tgttgctctt tatttaagga aacggcctgt tgattcatca taatgtcgat
     6421 agggtaacta cgcacatgac tttctacttg ctgcatcaat tttggacctt ctgtgtaaga
     6481 ggtaccaatc atattttcaa tccctagtgt ttcaacgact tgtccgccga aagaatcagc
     6541 gacaagtcct gtacggattc ctttacgtgc tgcatagata gctgcactac tagctgctgg
     6601 tccaccgcca atcactaata catcataagg ttctttttca aacaactctt cttcaatcaa
     6661 agggccagtg attttttcaa caatttgttc gatcgtcatg cgtccgctac taaattcttc
     6721 tccatttaaa aacacagttg gcactgccat gattttttta tcatcgattt ctttttggaa
     6781 catcccacct tcaatcatcg tatgagtaat atttggattt aatacagcta atatattcaa
     6841 tgcctgtaca acgtcggggc aattatgaca agtcaagcta acataactgt caaaatgcaa
     6901 tgtttgtgtt atttttttga ttttaagact caaagattca ctgattttcg gtgcccgtcc
     6961 actaatttgt aacatagcca gtaaaaaaga ggaaaattca tgaccgaggg ggataccagc
     7021 aaaaacgatt ccactttctt tgccaactgg attgatagaa aaactaggtg tgcgcatcga
     7081 atctcttttt tcccaagaga ttttaggaga catttctgct atttccttcg taaattcagc
     7141 tagtttttga ctattttcac tattatccaa actaacagaa aaaacaatag gagattcaat
     7201 cagtgctaaa tattgttcga gattttgttt agtttctttg tctaacatat ccaccaccta
     7261 aatctttcca acaagatcaa aacttggttt caatgtttcg ccactttctt tccattttgc
     7321 cggacaaact tcacctgggt gggtacgtac atattgagca gcgcgaatct tatcaatcag
     7381 tgcactagcg tcacgaccga ttccatctgc attgatttcc atagcttgaa cgattccgtc
     7441 tggatcaata ataaatgttc ctcgctgagc taatccttgc tcttcatcca aaacatcaaa
     7501 aatacgagaa atctgatgag acggatcacc gatcatcacg tattcgattt taccgatagc
     7561 atctgatgta tcatgccatg ctttgtgagt aaagtgggta tcggtagagc aagaaaagac
     7621 ttcaacacct aaagatttca atgtactgta ttgttcttgt aagtcttcta attcagtagg
     7681 acaaacaaaa gtaaaatcag ctggatagaa gcaaagaata ctccagttcc ccataagatc
     7741 tttttcactg acttctaaaa attctccttg atgataagca tgtgcattaa attcttcgat
     7801 ttttttccca atcaatgaca tagtaaaacc tccaataata attatttatt tataattatt
     7861 gtaatttagt tggagttgca aagtcaatgg atattttgtt atttaataaa gattactcaa
     7921 aaacgaaggt taattataaa aatagaagag gtcgcaagaa aatgctactg ctggaaatac
     7981 tcgggataac gttgagtaac agtttcctgt tctttcagca tcatgtgtaa gtgcaatccc
     8041 gtttgaaatc gagcttcttc tggtttttta tcaactaaag ccttgagtaa atcataatgt
     8101 tgttccatga tcgtttccca tggaagcccc tgaatggtca ctcgtaacca gcggaaacgt
     8161 tctaaatggg tattatggtc atctaaccag ttccaaactt cttttcttcc tgcaatttcg
     8221 aaacaagctt catgaaataa attatctgca cggaaaaaag cgcgttcgtc atttattttg
     8281 actgcttttt cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg tgtgtccaat
     8341 ttggaacaac attcaatcat gatttgctct tccaactgtt ctcggacaaa gcgagcattt
     8401 tctgcagaaa caagattaat tttggaaaca tacgtaccac tttgaggtat cgtatacacg
     8461 agttcttgct gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc gatagcaaga
     8521 tgttcctcca gtgccttttc agatattttc cttccaggtt ctaagtcagc atagataatt
     8581 tgtttacgaa tcgcttgata agcttgtgat tgcaagctgc ttttcatctt tattcctcca
     8641 aaatcatttc tatgaacgta gtataacaga agaggtctga aaaacaatct ggtatgtcac
     8701 attaactagt aaatttgtat aaatagtaaa attttggttg acatagcgat ttttattgca
     8761 taaaattcac ttgaatacaa aaactaatat gttagtttag gaggggaaaa atgattgatt
     8821 taaacaactg gaggaacttt atccctgaat tatctcaaaa agaagtagaa atacctcatt
     8881 ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc tgtctggatc catttcggtg
     8941 gcggaaatct ttatcgagga tttcatgcag aaatcgctca acgtttagcg gatctaggcg
     9001 aattgtccac tggtgtcatt gtttgtgaaa cattcgatga agaaataatt gaaagggctt
     9061 accaagattt tgatgatttg attcttgaag tggtcatgcc aagcagtgga gagttcgtca
     9121 aacgtatatt agctgcgaca gctgacagtc tgtattgtca tccagattgt tctgaacact
     9181 ttgataagat gattcgacac tttgaaaatc ctagtttaca attagttact tttgctatta
     9241 ctgaaaaagg ttatcgattg aaagatacaa ataataaata tataggaccg attgcagtcg
     9301 atataaaaaa tggtccaaag aagccatcac atacaatgag tattcttgct gtgctacttt
     9361 ataaacgttt tttatctgga gcttggccga ttgcacttgt ctctacagat aatttttcac
     9421 aaaacggtag tcaattacgt acttctttgc taacgatcgc aaaagaatgg caaaaggaag
     9481 gttatgtaca aaatacattt atccaatact tatcgaatga agaaattgtt gcgtttccat
     9541 ggacgatgat tgatcgtatc accccgaatc cttccttgtc agttagcaaa atgttggcaa
     9601 aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa aggaaataat attgctccat
     9661 ttgtcaatac agaggacatc cattatttaa tcatggaaga tcattttccc aatggacgtc
     9721 cgaaattcga aaaagctggg gtattcttga ctgatcgtgc aacggtagat aaagcagata
     9781 cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc tttagctatt atgggttgtt
     9841 tactggggta taggagcata gctgaggaga tgaaagatcc agatttatcg ctgctagttt
     9901 ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc tccaggtatc attgatccaa
     9961 agatctttct ggaagaagta attactgtgc gcttttctaa tccaaatatt cctaatatgc
    10021 ctcagcgaat cgccacggat acctctcaaa aggtggcgat tcgttatggt gagacgatta
    10081 aaaaatatat ggaaatagaa gttaaagacc cgcagacact tgtattcata ccattgactc
    10141 tcgctgcttg gcttcgatac cttattggca ttgatgatca aggaaaacca ttcatcccga
    10201 gtccagaccc actattgaca gaccttcagc ataagctttc cgatattcaa ctgggtacgc
    10261 aagatacatc acatattcat gagaaaatgg ctcctatttt gtcaaataaa gaaatttttg
    10321 gtgtagatct ttaccaagca gagcttggca gaaaaataga agaaatgttc atcaagatgg
    10381 tagcaggacc aaatgccgta cgaaaaacat tacaggatta cttacaagaa catggaggaa
    10441 atttatcatg gaaatgactt ttagatggta cggatcacag gaagaaaaga ttaccttaga
    10501 tgaaatcaga cagattcctg cggtcaaagg gattgtaggc acattgatgg atataccagt
    10561 aggagaggta tggcctaggg agaggatcaa agaactaaaa gaagaaattg agtctcgtgg
    10621 gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat gccattaaaa ttggattacc
    10681 ttcccgtgag cagtatatcg aaaactataa gcagacaatc cgaaacttgg cagaatatgg
    10741 aatagaagtc atctgctata attttatgcc tgtatttgac tgggtgaaat ctgaccttga
    10801 ctaccgctta gaagacggct catccacgtt agcttttata tctgatgata ttccagatga
    10861 tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat gaatttggat tgccgggatg
    10921 ggaacctgag cgcttagctc ctatcaaatc cttgcttgaa gcctatgcat cagtagacga
    10981 agaaaaactt cgagaaaatt ttgcctattt tctccaatca atcatcccaa catgtgaaga
    11041 ggtaggggtc aaaatggctg tacatccaga tgatccgcca tactcgattt ttggattagc
    11101 tcgaatcgta aagaatcgag aagatttaga ttggttgtgt cgcatagtag acagccctta
    11161 taacggaatc actctttgta cagggagtat tgcagaagat ccagaaaatg atgtatatgg
    11221 gattttagca gaattttgcc aaagagatcg aatcccattt gcacatgttc gaaatattaa
    11281 atttttagct aatcgagatt tctatgaggc accacataaa tccgaatacg gttcattaga
    11341 catgtaccgc atactaaaaa caatgtatga caacggattt aacggataca tccgtcctga
    11401 tcatggaaga atgatctgga atgagacagg aagaccagga tacggcttat atgatcgagc
    11461 attaggtgct gtttatctaa atggattatg ggaagcaatc gaaaaaggaa acaaaagtaa
    11521 agataattag tgaaaaaaac tgtaatttta ttatgtaaat aagaaggatg ttcaaattct
    11581 tgatacataa atccggaggt tgttaataag cattcggatt tatgtatctt atttggtcta
    11641 ggaagtaact tgattttctt ttttcgattg atccatgagt gcttcttctt ttaattcaaa
    11701 gatatattct gagatcctca taatcacttt tttccagtct tctggtgtga tgattccttt
    11761 taaaatatag atttttttga tatgagaagg caattgtttc aaagggcagt cgctgataac
    11821 gatgttgcag ttctccataa aatcattttg tattttgatc gattcatttg aaaaaatttg
    11881 ttctatctta acgatcagat ctttactgat ataaaaatca cgcgtatagt tcacaccaat
    11941 cacaattcgc ggctgttttt tgtagcgatc atacaaatgt actaggtctt caaccatcca
    12001 ttgcacattt tccttttcta aaataactgg taacaagtgt tgattttgtt ctctgaaatc
    12061 catataaaat tgttcaatgg cggtttggac ttctttcaat acgctatgtt cttgataaag
    12121 catgaaagta ggaaggggcg tatttggttg attgaagtta agaaatccat tataaagttt
    12181 atttcgtgcg aatgccaatg agtaaagaat cttttctttt tcaggtataa acaaatcaaa
    12241 tacagtacaa agggactcca ccatttctct tgaataaaga acatgaggaa cgtttgcttt
    12301 ttttaaaagt ttgtattgct ccaagcttgt ttcttccgat tcgattttcg tgatagaaag
    12361 tcgagctaaa atattcacta agatgcgttg ttcgcgacaa attggcagat taggtcgcaa
    12421 aatatctaca gagagatcaa taaaaaagtc aatatattca tactctgtga attctttttg
    12481 tacgtctttc acagaagttg caggaaaatt ctctttacat atttttaata gaaaatataa
    12541 tttatctatc ataccgctat ttagtcggtc aagtacttct tttttaaaaa aagtattcat
    12601 cataaaagcc actacttgcg gatcttcatc ataaaattct gtagcagtac ttgaaaagat
    12661 gttccaataa atatctaatg ctgcatattg aatttgtacc tcttttccag aaaaagactt
    12721 tctgccatta ttagaaaagg agatagacaa accgtactat gctaaaaatg cattaatctt
    12781 tttcattttc gtatacaaat aagaggcact atagtttgtt tgctgactga aactgataat
    12841 cgaatttgtc tgacagctga acaaagaagt caagatctga taactgactg attcgttgca
    12901 gtatcttcca aatagacgat aatagcagta agccggatca attccttcaa aattcaactt
    12961 taaaaaaggt ttttgcttat caatatgcag ttctgttaga ttgagacgct caaagtcttg
    13021 ttctaattct ttgatgtata gtaaaaaatt tgattcagac atatggaaat gat
//
LOCUS       DAZKMB010000078        12885 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000078 DAZKMB010000000
VERSION     DAZKMB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.078"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1135)
                     /locus_tag="ACG8V3_001966"
     CDS             complement(<1..1135)
                     /locus_tag="ACG8V3_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0300488.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHN"
     gene            complement(1257..1853)
                     /locus_tag="ACG8V3_001967"
     CDS             complement(1257..1853)
                     /locus_tag="ACG8V3_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0300489.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2053..4695)
                     /gene="alaS"
                     /locus_tag="ACG8V3_001968"
     CDS             complement(2053..4695)
                     /gene="alaS"
                     /locus_tag="ACG8V3_001968"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0300490.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5083..5514
                     /locus_tag="ACG8V3_001969"
     CDS             5083..5514
                     /locus_tag="ACG8V3_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0300491.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5559..6908)
                     /locus_tag="ACG8V3_001970"
     CDS             complement(5559..6908)
                     /locus_tag="ACG8V3_001970"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0300492.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(6978..8141)
                     /locus_tag="ACG8V3_001971"
     CDS             complement(6978..8141)
                     /locus_tag="ACG8V3_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0300493.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8270..8593
                     /locus_tag="ACG8V3_001972"
     CDS             8270..8593
                     /locus_tag="ACG8V3_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300494.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8776..10125)
                     /locus_tag="ACG8V3_001973"
     CDS             complement(8776..10125)
                     /locus_tag="ACG8V3_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0300495.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10194..11147)
                     /locus_tag="ACG8V3_001974"
     CDS             complement(10194..11147)
                     /locus_tag="ACG8V3_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0300496.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11249..12439)
                     /locus_tag="ACG8V3_001975"
     CDS             complement(11249..12439)
                     /locus_tag="ACG8V3_001975"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0300497.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(12639..>12885)
                     /locus_tag="ACG8V3_001976"
     CDS             complement(12639..>12885)
                     /locus_tag="ACG8V3_001976"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300498.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         3702 a         2714 c         2226 g         4243 t
ORIGIN      
        1 aattgtgatc aaacgtaatt tccaattggt aatctccgat tttttcttct atttgactac
       61 gtaagattct ttctagttct gtatcaacaa actggaattt ctcagggacc actacatcaa
      121 attgcagcgt ttttccttga tgaattactc taaaatcatg aatattaagc cctgtacctg
      181 tttcaagaag aatttgcttt aactgccgca agatattata gtatttttcg ttatcaacag
      241 gcattggatc caagtgacag acaagttcca catccaaaga ctttttaaag tctttttcta
      301 tcagatcaat aatcttatga gccacattca aattcattga ttcatctact tcgatgtgga
      361 ctgatgcaaa gcgtttattg ggtccatagt tatgcaccaa taaatcatga tagccaagta
      421 tatcatcgca agcatttaaa tgctcttcca tttcttcaat ctctgctggc gttgggcgac
      481 ttcctaatag ttcgtataca aaatttcgaa gcatcgtcaa tccactataa aggatatata
      541 atgctaaagc aaaacctata tatccatcaa tacgccatcc agttacccat tcgattgctg
      601 ctgaaagcaa tacagccagt gtagtaaaga catcattata actatctgtt gctgtagctc
      661 tcaatgtttc agaatcaatg gattgcgcaa taacctgata cattttgcct tgtgcaaatt
      721 tcaaaaagat ggaaatgatt aggacaaaaa agacgatggg tgttaaagag acattttctg
      781 gatggatgat tttttcagca gaagactcta aaaattgaaa tcctacataa gtaatgatga
      841 tcgaaacaaa taacccactg atatattcga agcgttcgtg tccataagga tgttcctgat
      901 ctgctggctt tgcagcaatt ttaaatccga tcaaagtcaa tatggatgaa gcagtatctg
      961 atagactgtt cactgcatca gccataatgg agacacttcc tgaaaaaagc ccgatcatta
     1021 gcttagagac aaacaagatc agattcgtaa ccaatcctaa gatacccgct agtactcctt
     1081 tttttgtccg ctgttgcggt atttgtttct ttttttcttc ttgtttctta gccaagtttt
     1141 ttaccccttc tagtttgctt gttgctcagg taaaaagatg gagaggacga ttgttttcct
     1201 ctcctctctt cctttttcta tctatcgtgc gtcctattac tattaaaaga tggtcattat
     1261 tctggtttca acggtgttgc tgcaaataac gcttttgcta gtaatggtgt tatgattccc
     1321 gcacctattg cttctggtat cccattgatt gctgcaaccc ctgctaaggt tttaaataaa
     1381 agagcagagt ctacaccata ggcatctgcc gttgcacctg tataaaataa tcccatgaat
     1441 cctaacacta agactgtatt gaccaatgaa cccagtaccc ctgcaaaggc tgagctgatc
     1501 gttattgatt tatttttttg ataaatccag tggaaaacca agcctgctac taagccaaca
     1561 agtactctgg gaactaccga gataatcgga ttcgtgaaca ctaatgtatc aaatggcgtt
     1621 gttggcatgg cataggcacg aacaattgta gcgattcccc aaaacaagcc aatcaccatg
     1681 ccatcttttg ggcctaatac tactgcagca ataattactg tgatatggat aattgttaaa
     1741 ctaatgaatc ctaatggaat aaaccccagc caaggaacca tcgcttgtac gatgatgatt
     1801 gctaacaaga tcgcacgtaa gactagtcga aaggtcttat ttctactatt cattttttca
     1861 ccccttttta tttatactaa tcgtaaaaat ttctgtcact ttcgtcaact aaagaccatc
     1921 cgaatccgac catttgatta gatgcgccat ccaattgaaa aaagtatgag ggcaagactt
     1981 gtttgtcttc gcccccataa tcatgtttcg ttcaatgttt gaacttttgt ttttctgtca
     2041 aacgtaaacg ccctattgtt gcgcaaccca ttctttcgca gctttaagtg catctggaat
     2101 accagcagga tttttcccgc cagcttgtgc catgtctgga cgtccaccac cgccaccgcc
     2161 tactttcggt gcgattgctt taatcaagtc gccagctttt agtccagctt catttgattc
     2221 tttcgtcatt gccgctagca gactgacttt tccttcttgt tcgtttgcta aaacaagaat
     2281 atctgaaata tttttttgtt tccattggtc agctaattga cgcagctgat tcatatcttt
     2341 cacttttact tgtgctgcaa tgattgtttt tccattgatc gtttctggat ctttgaatac
     2401 atcaccagct tgttgattcg ctaacttgct agctaattgt tcattttcct tttgtagctc
     2461 tcttacttgc tgttgcaatt gttccgcttt tgaaacaact tctttcaatt gaggtgattt
     2521 aacaattgtt gctacttgtt ttagttgttt ttcttcttct tcaagtagtt cgtacgcttc
     2581 tttacttgtt acagcctcga ttcgtcgcac gcctgcacca attcctgatt cagaaacgat
     2641 tttgaagatt ccgatgtttt ctgtatttgc tacatgtgtg cctccgcaca gttcgattga
     2701 ccagccgcca atatttacta ctcgaacgtc atgtccgtat ttttcaccaa acaaagccat
     2761 tgcacccatg tttttggctg tatcgatatc tgtttcaatt gtttcaactg ggatagcttc
     2821 ccagattttt tcgttgacga ttctttccat ttcagctaat tcatctgaag tgatttgacc
     2881 aaagtgggta aagtcgaatc gtaaatgtcc aggagcgact aaagaaccag cttgatttgc
     2941 atgctcgcca agtacttctt tcaatgcctt gtgcagtaga tgggttgctg tatggttttt
     3001 gatgatccga ttgcgcatcg attgatcgat caatagatga taggtttgtc cttcactcat
     3061 tggtgctttt acttctacgg tgtgcataaa ttgaccatta ggcgcttttt gaacatctgt
     3121 aacaactgct acgatttcgc ctacttcatt catgatccat ccttgatccg ctacttgccc
     3181 acccatttct gcatagaaag gtgtttgatc aaagatcatt tgagcttcac cttctgcgac
     3241 ttcattcaaa agagactcac ctttaacgat gaccaataat tgactatctg attcttgttc
     3301 tgtgtatcca atgaatttac tttctacttt gatatctgtc aataaagcag attgtacccc
     3361 cattgatttt tcagtactgc gggcagagcg agcacgttcg cgttgtgcat ccatttcttt
     3421 ttcaaaacca gcatgatcaa ctttcagtcc ttcatcttct gccacctctt ccgttaattc
     3481 taccgggaag ccatatgtgt cgtatagttt gaagatgtct tttcctgcta gagtcgtccc
     3541 gttttcgttt tttacttctt ggatcacttg atttaagatt tccaaacctt cattgattgt
     3601 ttcattgaaa cgttcttctt ctgtacggat aactttttca ataaattctt tttgttgaag
     3661 tacttcagga taataactta ccatgatttc cccaacaaca gggactaaac gatataagaa
     3721 tgcttgttca atacctaatt ttttcccatg catgacagca cgacgaagca aacggcgtaa
     3781 gacataccct ctcccttcat tcgatggcaa tgcaccgtcg ccaatagcaa atgaaagggc
     3841 gcgaatatga tctgcgatta ctttgtaaga aatatctgtt actgggtttt caccatattt
     3901 tacttgtttg cttagctttt ctacggcgtg gataataggc ataaacaagt ctgtctcaaa
     3961 gttcgttgac gcatcttgaa cgattgagac catgcgttcc aatcccatcc ccgtatcaat
     4021 gtttttatga ggaagtggtt caaatgtgtt atcaggctta tggttgaatt ctgaaaatac
     4081 taagttccag atttctaaat aacgttcatt ttctcctcct ggatagtttt caggatcgtc
     4141 ttctgctaaa tcgttataag cttctccacg gtcatagaaa atttccgtat ccggaccaca
     4201 tgggcctgca ccgatatccc agaagttgtc ttctacatca ataatgtgat ctagtggcag
     4261 gccaacttct tcatgccaga tgcgttttgc ttctgtatct tttggataaa ctgttacata
     4321 aagtttttct ggatcgaatg ccatccattt tggatcagtc aaaaattccc atgcccagtg
     4381 gatggcttca tttttgaaat agtcaccaat cgaaaaatta cccagcatct caaacatcgt
     4441 atggtgccgt gctgttttac cgacattttc gatatcattt gttcgaatag atttttgggc
     4501 atttgtgatt cttggatttt ctggcacaac tgaaccatca aaatattttt tcaatgttgc
     4561 aacaccagag ttgatccaca ataaggttgg atcgttcacg gggatcaaag acgcactagg
     4621 ttcaatcgta tgtcctttag attggaagaa atcaagaaac atttgtcgaa cttgtgcact
     4681 acttaattgt ttcatgtcat tacctgcttt ctataaatta aatacaaaaa aacaccatca
     4741 cagccaagga cgaaaactcg cggtaccacc ttgattgcaa tgatgtgtaa tcattacctc
     4801 ttaaactcga taacgctgag ctgcgtcggt atttctacca aaatagaaaa agggagcaat
     4861 ttgattagtg actattcttt tttcagccaa gaaagaattt tctgtgagtc ttaaaaaaca
     4921 aatcatatcc ttttcgtact taattagtat acgcattcta ttgatcactg tcaatatctc
     4981 aatttttatt ttcatttcct tttggtcata ctccacgatt tttggtacga taagaataaa
     5041 ggaatcaata catctctgct attcatgaca aggaggaata tgatgaaaaa aatagttgga
     5101 gcggatgaat ggatacgcgc agctagctgc tttttgagat atcaagtttt tgtattagaa
     5161 caagggattc ctgcagaact agaattcgac ggaaaagata tcccgcaaca aacctattat
     5221 ctatttcttg atcaaaaaag accagtagct actttgcgtt atgaaaagaa aacacctact
     5281 tgcttcaagc cggaacgatt ttgcgtagca aaagaatacc gaggaaaagg gttaggcggt
     5341 cagttgcttg aagctgctga aaaacgggcg gtttcagaag gttgtcagac tagttatgta
     5401 gtagctgaaa tgacggctgt agatttttat gaaaaacaag ggtatcatac ttgcacagaa
     5461 ccttttattg aagatgaagt actttgtatc ggaatgaaaa aaagtctagc ttaaaaacaa
     5521 gagattgtga cacgcctttt gtcacaacct cttgtttttt atttatgtga atttttctta
     5581 tttttacgag cagtacggct ttgctgtctg cgttcgattt tgcgttcttt tttatttttt
     5641 tctgcaattg cccaatcgat ttttttctta taaccaggct tgatcttttt ctttttcttc
     5701 ttcacaagac caatcagcgt gggatctaat acatcttttg atttttctcg tttggcacgt
     5761 ctattacgat cgtatgtttt gacgatttcc ccgtttttga tttcttttgg ttcaaatgag
     5821 acgcccaatt tttctatcgc atcaatggct tgctcatcat ttggcgaata taacgtaatg
     5881 gccgttcctt ttaagccatt acgtccagta cgtccgacac gatggataaa gaagtctaga
     5941 tcttctggaa tttctgcatt aataacatga gagacacctt cgatatcgat ccctctagca
     6001 gctaaatcag tcgctacaac atattgatac tccagattct gtacttgtcg catcactcgt
     6061 tttctctctc ttggggtgat gtctccatga atctttgcta ctttcaagcc ttggtttttc
     6121 aagtaatcgg caatttcatc cacatgctgt tttgtattgg caaatacgat tgctaaataa
     6181 ggatgaccaa ttgtcagcag ttgataaatc atttgattct catctttacc ttttgttgaa
     6241 atcagccaat tttcaatgtc ttcagaaata actgcttttg gtttgatttc ttcaatgatt
     6301 ggattttcca tgtattttcg taagaatgga cgaagttttt ctggaatagt ggcagaaaaa
     6361 actaaaaact gcagcttttc tggtaatcga cttgcaattt ggtctacttc atttaaaaaa
     6421 cccatatcca atgtcatatc cgcttcatcg acaacaaatg caaatgcggt gtgaatcttc
     6481 agcgcttgtt cattcatcat gtctaatatt ctaccaggcg tgcctactac aacctgtggc
     6541 tgttggtgat gcagacgatt taattgacgc tgtttgtctg taccgccgac aaaattcgag
     6601 acacggatct ccggtgtaga aaatttagcg atttgtgtag ctgcttcata gatctgtgca
     6661 gctagttccc tacttggagc tgtaataaca acttgtactt cctctttttc tgcattgatt
     6721 ttttcaaaca aaggcaacaa aaacgtatgt gtttttcctg taccagtttg agattgccca
     6781 atgatatttt ttccttttgc aattaaagga atcaaacgtt cctggacctc tgtgggctgt
     6841 ttgaagccct ttgcggttaa cgcttcattg ataaacggtt tgaagcggaa tatatcaaaa
     6901 ttattcattc gtgcacctct tctctttaaa ttatcacgac tttggcatta taacacagac
     6961 aaagcgtttt tgctcagcta atttcgagaa tcatcatcct ttgtttggcc aatcgtgcta
     7021 acagtatatg tgtgactagc tttttcataa aacagaatcc gtttttttct caaaaatgat
     7081 agcaagatat ttaaaataaa aataccttgt agtaacaaga caccgagaga aatgaatact
     7141 gcactcatct gcaacaccgt attcgttgtc gaattcatga tttgctgaag caactgcccc
     7201 aaccagctta aaccataaaa aagaaagtac agatacccgt agcggatcat caatgctttt
     7261 aaagaaattc gttcgcggtc ttcttccacc aaacggatac gcacaatttt tttgccaagt
     7321 gtccatccat tggttataaa cggtacaatc ataaagtata cccctacttc taataaaagc
     7381 caccagatat gagaagtaac tgcccctgaa tcgaacgcaa ttttaaagaa aatcgccgct
     7441 accaaattaa taagagataa aaatgtccag tcgatcaaat aagcgaccaa gcgtcgagtc
     7501 aatgagacag tctttccttt ttcataagaa agttcatcca ttcgtcttct tgtaggaagc
     7561 ataaatgtaa ataacggcgt aacgacaaaa ccaatgattc cacccaatgt atttgtcact
     7621 aaatcattca catcaaataa gcggtatggt cttgggtaga taaaataaag tccacttagc
     7681 tgcgtcaatt caaaaaatag agaaaggcag aaactagcaa tcatcgtttt cataaaagaa
     7741 agccggaaat aatatcttaa ataaatgcca aacggtatga gtaataaaac attaaaagct
     7801 ggttccaaaa atacactctg cttcattgcc ggcaggtagg tactaggttc agacaagacc
     7861 aataccgtgt tgtctctgaa atgttgccat gaagcaaata aatgcagctc catctttggc
     7921 cctgtcattt gggcaaccgt ctcctttgcc ggaagtggta agatcaccaa aaaatacgca
     7981 cacaataaat aaaaaacgaa agaatacaaa ataactgccc ttgttactag aaatgttcca
     8041 tactttcgat attcataaat aaaaaaaaca gatgagatcg ctaaggctaa aaatggaaaa
     8101 acaatcagcg caaaacgaat cggttcgata taacttgcca tatgactcct ccttgattct
     8161 ttgtatctct cttatcttag cagtcctttg ctgtttcaag caagtaacga ggctattatt
     8221 tctcatttct ttcaaagtaa agtaagataa acacaaaggg ggtgtcgaga tgctgaatat
     8281 gtactttgtt tttggcgtac ctattttttt actgttcctt tatgctacga tcgcttatgt
     8341 aagaaaaaga actacaattc attatttagg atttattctc ctgattattt cgggtttcat
     8401 gctagtcttc aatcttcaga cttggcagca agcacttctt gaaatggata aaatgactcc
     8461 acatgctttg tctaaagtgc ttggctaccc tgtctatctg atttggcttc ccattttcat
     8521 ttctggttgc ttggttttat taaatattta tcgcggcgta cgccgaatag ttcagttacg
     8581 caaatcaaag taaaagacag aaatcggttg agcccgattt ctgtctttta ctttgctatt
     8641 tcatcgtaga caaaacgaca tttacgtgat caaaattaac gagtactttt gacatttctt
     8701 ccacgtactc ctcatttaaa taaaatattt tgtttggcag agaagtgcag ttctaataaa
     8761 attcgaatgt tttagctatc tacgttttaa atagagataa atcgggatac cagcaagtgt
     8821 caatactatc cccatcgaag ctaaaaaggt ctgagtgata agcgtgcttg atacgataaa
     8881 agtccctcca atgattgcta ctagtggaat aaacggatac attggaactt tgtatggtcg
     8941 aaacaaatca ggttcttttt tacgaagaag aatgacacca acaaagacca tcgtataaaa
     9001 aatccaaatg acaaagatca gcatatccgt tagtgtatca aaaccaccga tcatcatcat
     9061 gccaatagcg atgactagct ctaagatacc tgcggctaca ggaactttag ttttatcatg
     9121 aagtttagca aagagcttgc taaaaggcag gttgttttct tttgccataa cataagggag
     9181 acgcattcca gtaagggtat agccattgat ggttccataa acagaaatca agataccaac
     9241 tgtgaccagt cttccaccga agccgccaaa aatcattttt gcaacgtcac ttgccgcatt
     9301 gctgtttcct gcgactccgt cgatagatgc ggttcttaag aacaccgcat ttactaataa
     9361 ataaacgatc atgattccga tgattcctaa agaaattgct tttggcaaat ctttcgccgg
     9421 ctttttcaat tctcccgaaa tatttcctac gtgtatccaa ccgtcgtatg cgaacatcgt
     9481 tgctagcaag cccgctccca aggctgagaa aaatgaaaga ttttctcctg cttggatcgg
     9541 aaacagttgg aagtctactc cttcttgtcg gaacaaaccg aaaatgacga tcacaaacaa
     9601 aggaataagc ttgcagacaa gcgtaatcga ctgaaaagcc cctccagctt tagaacctaa
     9661 aaaattgatc aacaggatcg atacagcagc tgtaacagct acgggaacaa tcattgactg
     9721 ggaaagacca aatagattga cgaactgtgt accgaaaatg atcgacaagg ctgctacatt
     9781 tgctggaaaa taaatcacga cttgcgccca gcccaataaa aaagctgctg tatttccgta
     9841 aatccgttcg atatatttaa tcatccctcc tgtttctgga atagcagctg ccagttcagc
     9901 tcctgttagt cctgcacaga cagaaatcat accaccgaga aaccaagaaa acatatgcaa
     9961 actggcggat cctgtaactt ctgctacgct tgcggcttta aaaaataccc ctgcaccgat
    10021 cactgtgccc ataacagtcg ataaagcagt aaaaaatccc atcgtacgtt ttaattcatt
    10081 ttctattttt acttctttta ctggcgtggc ttgatattct tccatccctt gtcccctttc
    10141 ataccatagc caatcccaaa agtataaaaa actttttgga tgtggttctt gttttatacc
    10201 gtctctacca atttttcttt cagtttttcc aacatatcct ttgtcatcac atctaagtcg
    10261 tatttcggcg aaaagcccca ttcctcttgc gcacaagaaa catctagtga atttggccaa
    10321 gattctgcta ttgactgacg tacaggatcc acatcataat ccatttcaaa atctggtatg
    10381 attttttgaa tagatgcttt gatctcttcc gggtcaaaag acatcgccga tatattgaag
    10441 gcatttcgat gaaccaatcg atcaggatct gcattcatga gttttataat ggcatcgatc
    10501 gcatctggca tatacatcat atccattgat gtaccttgat caatgaaact agtgtacttt
    10561 ccttctttga ttgctgaata ataaatatcg actgcgtagt ctgtcgtacc gcctcctggc
    10621 aaggttgcat aagaaatcaa tccagggaaa cggacaccac gagtatctac gccatatttc
    10681 acataataat aatcacatag tagttctcca gctaccttcg ttaccccata catggttgta
    10741 ggacgctgaa gagtatcttg tggggtatga tcttttggag tatttggacc aaacgcgccg
    10801 attgaacttg gcgtaaaaaa tttcagctgg aattctctag cgacttccaa tgcattgacc
    10861 agcccagtca cgttcaaatt ccaggctaat tgtggttttg cttctgcaac agctgacaaa
    10921 agagcagcca aatgaatcaa tgtgtccact tgatattgtt ctgctatgct gcgcattttt
    10981 tcgtaatcca ttacatctag gatctcaaag atatcgtttt tctcaatgac ttcaggcatg
    11041 cgtatatctg tcgcaatgac attttcactg cctagttctt gacgtagccg tgtgatcaac
    11101 tctgaaccaa tttgtcctaa acatcctgtc accaatactt ttttcatgat gatcctccct
    11161 gatctttttt actactatct tcagatgatt aacaatatat gcgtgaacat gttctctaat
    11221 tttaaataga cgaaaaaact aaatttagtt aaataatttc catttctttt cctatttttt
    11281 gataaactgc tactgcttca tcaagcattt ccctcgtatg ttccgcagtc ggcatatttc
    11341 tgatcctgcc tgtcccaaga ggcacagttg gataaacgat tggtttagca taaatcccat
    11401 gttcgatcag cttcttagag aaagtctgag tcactttttc gtctcccaaa atgactggag
    11461 taataggtgt ttcagacata ccgatgttat agcctacttt tttcaattct tctttaaaat
    11521 aattagcatt ttcccaaact ttttcgacaa gttccggatg ttcttgcatc aaagtaatcg
    11581 aagccaatgc agctgcagct gcacctggag ttagcgaagt agaaaatagg aatggccgac
    11641 ttctagcttt caaccagtcg attagtgttt tcgatccagc tacataaccc ccaacaacac
    11701 ctatagcttt cgataacgtg cccatttgca tatcgatttt gtcgcttaat ccaaaatgct
    11761 tgacggttcc tgcacctttt ccagttacgc ctgagccgtg cgcatcatcg acgtaggtga
    11821 tcagtccata tttttcaaca atcggaataa tttccggcaa tcgtgcaatg tctccatcca
    11881 tggagaaaac accatctgtg atatacatga tttttttata tttcttactt tctatcgctt
    11941 cctttgcttt tttctcaagg tctttcatat cttgatgttt aattcggatg atttttgctc
    12001 cggataagcg gcaaccgtca ataatagatg catggtttaa ttcatccgat aagatagcat
    12061 cttcttttgt cattactgca gagatagcgc ccatattgca gttgaatcca gattgaaagg
    12121 cgattgctgc ttctgtccct ttgaattctg cgattttttc ttctaattgc tgatggattt
    12181 tcagcgaacc attgatcgtt cggactgctc ctgctcctac accataagtt tcggttgctt
    12241 caatacatgc ttttttcaat tcttcgcgat tggcaaatcc aagataattg ttggatgcta
    12301 gattgatcat cttttttccg tctacgatga tacaagcacc attttcactt tctagtatgt
    12361 cgatcgtatt gtataacccc ttttctttca gttgatccaa ttctttctct aaatacattg
    12421 ttagttcttt ggaagacatt gtctccacct ccatattcat ctgtcttgca gaacatacca
    12481 aacatatatc gtgtcattca tctacaaagc aagtatgcta tatttttgat aacgcttaca
    12541 ctgattatga gaattattta accacatttt ttatttacag tatcttttta ctttcaaatg
    12601 acttatatag tatgaacaat taccttttag ataatgatct attttactct acctgggcag
    12661 ataaatacaa cacaaccatt gatagaagcc ctttcgttaa aatcaattgt attctgaata
    12721 tttaatttat ttattgcata gaccaataaa aatacataaa atttccatct tcgtttatca
    12781 gcaattttta attttactct ctgaatattt cttaaaagtt ttacttgttg aattcgaaaa
    12841 aaggactgtg acaaaagtct tgtcacagtc ctattcttcc gaata
//
LOCUS       DAZKMB010000079        12809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000079 DAZKMB010000000
VERSION     DAZKMB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.079"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..177)
                     /locus_tag="ACG8V3_001977"
     CDS             complement(<1..177)
                     /locus_tag="ACG8V3_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0300499.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
     gene            319..933
                     /locus_tag="ACG8V3_001978"
     CDS             319..933
                     /locus_tag="ACG8V3_001978"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0300500.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1122..3002
                     /locus_tag="ACG8V3_001979"
     CDS             1122..3002
                     /locus_tag="ACG8V3_001979"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0300501.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3081..3308
                     /locus_tag="ACG8V3_001980"
     CDS             3081..3308
                     /locus_tag="ACG8V3_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0300502.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            3313..3855
                     /locus_tag="ACG8V3_001981"
     CDS             3313..3855
                     /locus_tag="ACG8V3_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0300503.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4082..4798
                     /locus_tag="ACG8V3_001982"
     CDS             4082..4798
                     /locus_tag="ACG8V3_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0300504.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(4858..6693)
                     /locus_tag="ACG8V3_001983"
     CDS             complement(4858..6693)
                     /locus_tag="ACG8V3_001983"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0300505.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6889..7887)
                     /locus_tag="ACG8V3_001984"
     CDS             complement(6889..7887)
                     /locus_tag="ACG8V3_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300506.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8106..9326
                     /gene="sstT"
                     /locus_tag="ACG8V3_001985"
     CDS             8106..9326
                     /gene="sstT"
                     /locus_tag="ACG8V3_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0300507.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            9473..10108
                     /locus_tag="ACG8V3_001986"
     CDS             9473..10108
                     /locus_tag="ACG8V3_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0300508.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            10299..11039
                     /gene="truA"
                     /locus_tag="ACG8V3_001987"
     CDS             10299..11039
                     /gene="truA"
                     /locus_tag="ACG8V3_001987"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0300509.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11184..>12809)
                     /locus_tag="ACG8V3_001988"
     CDS             complement(11184..>12809)
                     /locus_tag="ACG8V3_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300510.1"
                     /translation="KNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIG
                     LDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLT
                     MVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCY
                     EIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLK
                     ATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLF
                     LFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNG
                     FSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAP
                     EDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSN
                     NKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIA
                     SRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4284 a         2034 c         2508 g         3983 t
ORIGIN      
        1 atcatataga tcatagatat ccttataatg tgtataaaaa gtcgaacgat gtacatctgc
       61 cgtttctgtt agttcgcgta tagtgattgc ttgaatcttt ttatttacta ataatgtgga
      121 tagtgtttca gccaaagctt tttctgtttt tttgacgcgt ctatctgtct ttttcattta
      181 cctatctcct tatccgacat atcaaagcaa agctgtcgct taatcgacaa acttatagat
      241 gattgattat agatagtaga cactgtgtct attataatgt aagtataaca caatcattga
      301 agttgaggag gcgaaatgat gaaactaact tttaaaccct tagaaaagaa agattacaaa
      361 aaagctatct attttgctgt tgaaggaatg aactttgaga gatatatgga aaatgaactt
      421 ctgctgaatc tttacggaag atattttttg tatatggagt tgaatcgagc aactcaaatt
      481 cttgctttgt atgcagagga cgagttggca ggtgttttgc tggcggagat aaaaggagaa
      541 aaaaagaaac atcgttcttt tggacagcaa atatatgtta agatattcga ttttttacaa
      601 aatatatttg ctaaagaaag taaaggagct tacgagacag caaatgaaca aatgcttgca
      661 gcatatttaa agaagcattc gccagatgga gagatcattt ttttagctgc aaatcctgat
      721 ttgaaaacaa aaggcatcgg taccaagttg ctaacagaat ttgaaaaaag agagaaagga
      781 aaagaaatat tcctttttac agatagtggc tgtacgtatc aattctatga acgacgtgga
      841 tttgaaagac taaaggaaaa aattagcaag ataaaaatct tagataaaga aatagatctc
      901 cgatgtatgc tgtacaggaa aatcattcaa tgaaaatttc gatagaaacg acaatagttg
      961 gcaagatcac ttttagtgag tcttcttgct aactattttt tgtagtgtat tctaccaaaa
     1021 gaaaaccgcc tttttgacta aacttgatag tggtcaattt tttatgatgg ataagtatct
     1081 atacttagtc tcgtaagaat gaaaggaaaa gagggagaaa gatgaaatta caaaagtgga
     1141 tccaagagca tcgaaatcat atcaccgcct tatcagctgt gttgattatt tttggtttta
     1201 tcggaaaatt tactggtttt gtcttgttct ataacattgc tctagccata gcttctatta
     1261 ttgcggtcat acctattatg atccatgcgt atcaggcagt gaaagtcaaa gttatctcaa
     1321 ttgatttact cgttactatt gctgtgcttg gtgcctttat gatcggagaa tacaaggagt
     1381 cagctatcgt tacattcttg tttttgtttg gtagctatct ggaacaaaaa acattgacca
     1441 aaacgcggga gtcaatcaag gctttgaccg agatggcccc atctactgct agtgttgtcc
     1501 aagaagatgg aacgactgaa gaagtggaaa tcgatgacgt ggaagaaggc gatatcgtct
     1561 tagtaaaaac tggtggagct attcctgtgg acggacgagt cattgacggg aaagggtatg
     1621 cggatgaagc cgcagtgact ggtgaatcac gacaagtcgt caaaatcagt ggagatgaag
     1681 tcttctctgg aacgatttta gcagatggct acgtgacgat tgaagcaact aaggtcggag
     1741 atgatacgac ctttgccaaa attatcgaat tagtagaaga agcacaagat accaaatccc
     1801 atgcggaaaa attcattgat cgattttctc agtattatac tccaaccgtt ttacttattg
     1861 cgttggttgt tggtctgatt agtcgtgatt ttcgtttagc aatcactgtt ttggttcttg
     1921 gttgtcctgg cgctttagtc attggtgcgc ctgtttcgaa tgtagcagga atcggtaatg
     1981 gagcgaaacg gggcatctta gtcaaaggcg gtgaagcaat ggatactttt tccaaaatcg
     2041 atacttttgt ctttgataaa actggtacat tgaccaaagg aactactgct gtatccacta
     2101 tcaagaacta tacagaggat gaaggggaca gcttagcttt agtggctcga ttagaaacgt
     2161 tatccgatca tcctctgggg cgcgccgtga tccattatgc tcaggagaaa gacattgatt
     2221 ttcaacaatt agcagtcaaa gataatcaga caatcaaggg acaaggaatc acagctgaga
     2281 ttgatggtca tacagtttgt gcgggcaaca ccaaattgat tgccgctcac aaactttctc
     2341 tcacaccaaa gcaagtacaa gatttacatc aattacaaca aacaggcagc tcggtcatta
     2401 ttgtagcaat cgacaaaaaa atcactcaaa tcataggtgt gtcagatgtg attcgaccag
     2461 aagtcgctga acaattagct aagttgaaac aagcaggagc aaaacagtta gtgatgctga
     2521 caggtgataa tcagatgact gctgattatg tagctgatat gttaggcatt gatgaagtcc
     2581 atgctgaatt attaccagac gaaaaggttc aatttgtgaa aaaataccaa gaacaaggat
     2641 tgcgcgttgc ttttattgga gatggaatca atgattctcc ctctttggct gcggctgata
     2701 tcgggattgc tatgggatca ggaacggatg ttgctatcga aacgagtgac gttgtcttga
     2761 tgcaatctaa ttttgaatcg ttggttcatg cctaccgctt ggctaagaaa actgttttaa
     2821 atacccgtga aaatgtagtg attgccattg gtgtcgtctt atttttactc aacggcttat
     2881 ttgccgggtt tatttacatg gctagtggga tgttcgttca cgaagctagt atcttgatcg
     2941 ttatctttaa cgcgatgcgt ttattgtatt ttggacacga aagcaaagaa gaaaaatcgt
     3001 aaaacttgat aagaatcaat ttttttatgg aagctgtaat ctagaataag actatcaaga
     3061 aaaaggaagg aagaactacg atgaaaaaaa ttattatgca attagataca ctagcttgtc
     3121 caagctgtat gcaaaaaatc gaacatgcgg tttctcaaca gcctggggta agcaatacga
     3181 aagttttgtt taatgcgagc aaaatcaaag caaactttga tccagatgaa gtccaccctg
     3241 aacaattagc tgcagttgtg gaaaaattag gttatgaagt ccaatcagca aaagtaaaag
     3301 aggcgtaaat caatgaatat agtggaagaa aaatttgttc aagaaacaaa acaagcagat
     3361 atcgaccatc ataaaccaac tgctggcgcc atggtggggc atgtattagc aaattttaaa
     3421 ttagaagaaa tgaaattgat ccaagcacaa ttgtatgttt tgggtacatc ttcgcaagaa
     3481 ttaagtaccc ttttttcaga atatgctgat caagaagcga gatggttcaa taaaatctct
     3541 catgagttag tgattgaaaa tgaaatcgtt ccgtctactt tagaagaagt catgcgttat
     3601 ggaaaaattt acgaacatgg tcaaaacaaa tatttaccag cctcaacaat gttggaaaac
     3661 tttgcaaaag attttgattt ccaaaatgtc tttatcacac gggcaatcaa attagctgaa
     3721 aaagaagaaa aatatggctt gcaacaattg cttgttgaac ttttaagttt caataaagaa
     3781 atcatctacc aagtacaaag actccaagga aaaactgtcc gacaagattt ggatgaggaa
     3841 gacgatgatg actaaaagtc tatgtttctt caaatacaaa tataaagcgt gaggtaaatt
     3901 ggaaaatcac gaagattaaa ataaaagaag cacccgctga tgtattacag acaacatcag
     3961 cgggtgcttc tcagttttta gaagatttga aagattgatg agaaaatacg aaaaatcaat
     4021 aaaatcacat tgcttatttg atagaataaa gaagtgggag taatcgaaag gagtttttta
     4081 attgaaaaaa aggattttag taacaattgg tctagctttt ttgttagctg gttgtaccaa
     4141 cgggaatgaa tcgaaaaaag aatcaacaaa agaaacagcg aaagcaacag ttgcaactac
     4201 tacaacaact agcacagaga gcaagtcaac tgcagcaagt acagaaacag cagatacctc
     4261 tacttcgtca gagtcttcca aaacgactga aacaactcaa acatctactt caagttcttc
     4321 tgtagaggct tcatcttcct cttcagaaag cagcgaacaa gttgaacaaa cactgtggaa
     4381 tgcagacaaa tccgcacaat tagaggcatt tatgaccaaa tggggccaaa caatgggaca
     4441 acaatacaaa agttatacga atcaaatgag tgtggactta tatggtctta aagtccctca
     4501 agctattttg aacggggagt ggaagatggc tattggtgga gtgcctgttt ctgcagaatg
     4561 gtcagaaagt gggacaggtc aagcagacta tcaaatcaca gccgtctatt ccgatgcaga
     4621 gacagaacca tatctgaaaa agcacgtcta tctattcggg tttcagcaaa atcagccaaa
     4681 agtattggtg acacaacaaa atcaggggaa tcccgataat tatctttact tcaacgagac
     4741 agcgaataat gaattgaaaa atggctttaa tcaaatcgtt tatggacaag caaattaatg
     4801 agataaacaa ggggttgtga catgtctttt atcacaaccc cttgctcagt ttatttgtta
     4861 gtcttttatt ttttgtgtcg cccatgttgc actgtactgg ttacctgcaa caagatcata
     4921 ttgtatcaga cgataatctt ctagaatttc tttttgtttc tcagttaaat ttttttcttg
     4981 gataatttga ctatcctctg taacaaagac ctgttttcca ttaaaacgat tagacaagct
     5041 ttctgtagga tcaattgtta atgcaggtag atgattagat acttctgtca acaacgcata
     5101 gtaaggagtt actttgatat tcgcatgctc aagagccata gcaccaaagt tattaggagc
     5161 aacaacttct ttatttaacg tagctgcttg ttcacgacta tacttgttgt tataaatgaa
     5221 ataatctgtc tggtgctgtt ctaatccata ttttttcgaa tcattcccac tatagattgc
     5281 gggtaaatgg tcaccataaa aaacaaaggt aatcggttta tcgattttgt cgatttcttg
     5341 gatgaatttt tttactgctg tatcggtata gtgaaggcct tgcatataag tttgtaattc
     5401 ttttcttcta ttaggaatga cagcagttcc ttcagcagtg tagttcaatt cactatagta
     5461 atcatcataa ggcatatggt tttgcatagt ggataattgg atgaattgtg acgtttccgt
     5521 attgctttgt aaagctttca aggtttcttt gtatgctgaa tcatccgata tgcgtgggct
     5581 ttgttctatt ttatccgtat aagttaattt atccggactg tctaaataat agaacctgtc
     5641 aaaaccaaat ttatcaaaca cttgttttct gcgataaaga gcagctgtaa aaggatggat
     5701 ggctattttt tcatcgaata gatcggtaat atttggactt attttctgac gctccactag
     5761 ctgggtataa ggtgtaacta acgaagcaga caggttactg atactcaagc ctgtcagtcc
     5821 ttcccattcc atgttagctg ttccgccacc ataaccgacg gataacatca atcctcctgt
     5881 tccttctgtt ataatgcttt tgatgtaagg aatagggtct gtaggtactg tcagattagg
     5941 gacacgtgca ggatcactga agctttcact taaattgaga acgatcgtct gattgtttaa
     6001 ccagtcactt cttgtttggt tgatttcatc tgcctttgtg ttgtactttt tcattattcc
     6061 attaattttt tctttcgaat agccagcagg ttcttccatg attttaatgt cgatattgtt
     6121 taggaattga atgactggtc cgttatcttt tacggcgcct tgctggttaa agaaagtctt
     6181 gtttacttta aataaattaa acagcaagaa agaaggcgaa tttttgtgat taatgaaaaa
     6241 gacacccgaa aaacatccta ataatacagc gatactgata aatcgacgtt tccaatgaat
     6301 cttcaaggaa tactgttttt gtagtctacg ttgtacaata atactagtaa tcaataatag
     6361 gagaaggatc aatccgccca cgattaataa gactgggcta accatcgata atagttctga
     6421 caatccggtc agcattttta aatctgaagg aaagacaggc tcttcccgca tttccatttt
     6481 gattactgtc gaaatagtga gcagtaagtt aatgatcaag gtagcagtca aggcgtacca
     6541 aaaacgattg gaaatcaaca atagtaagaa gaaaaatccc ataataatga tgattgtcag
     6601 gatcatgggt gcttgtcgct gagtaaatat ttttaaggca acctggatag tttctttcca
     6661 accttctttt ggttctaaaa cgaaaatctg cacgaaggta aagcagtaaa aaaatgctgc
     6721 aactaaaaaa taacttttat gaacaaaaag ccaatgaatt ctttttttcc aacgctgagt
     6781 taattgttca atcgaatcaa aagcgaagta ttcgatgaaa cgatgaatga aacggatttt
     6841 ttgtatataa aataaaaaga taactaggag ggacactgct aataaatatc aaataaaaaa
     6901 ctgcccgata tttatcagcc atgccttccc tgagtttgga aattcacgtt tcataaatgt
     6961 agatacgcga taagaaacta aagggaaatt acatataact tgagtgacaa tcaaaaatac
     7021 agcaagaaaa tttcctcttc cttttatacc taatttatca gatagcattt ctaaaaataa
     7081 aaacgaagag aaagtataat acattgcgaa gagagaatat ggtttagaaa acctcagcgc
     7141 tgtatctgta tttcctctga tgatgagtga aatttgtgtc atcaaaataa tcaaaccgaa
     7201 taaaacaatc aagctgataa atacttgagg aagcttatag cggacctttt ctttccagtt
     7261 ccattctttc aaaccataac ccattaaaaa taaataaaaa atccataaaa tactctgtcc
     7321 attctgtaat gcaaacgggt ctttattaaa tagagtagag cagccaacta atccaattgt
     7381 taaaaataat agtagatatt taattgttgt tttcgataat ttatttaaaa atccaactgt
     7441 ataaggaata ctacaaaaaa gcaaagtaca agaaacagca taagtaaagt aattctgact
     7501 gataggtgtt aggataagcc aataatcacg aatattcaag ctgccagctt gtacgtaaaa
     7561 aaagatattg caaactagtg tggctattag gaatataaag gcaaaacgac ttaaataatg
     7621 taaaactttt aaacgggcga ttttttgata ttggtaaccc atgtaaagtg ggatcaaatt
     7681 tgcaccaata acaacggaag tttgtaaaaa tgtatagatg tagaatcgac cagggtttgt
     7741 tgcatatgta ggaatagtga ctgctgaaat caatatacgc tgtaaaacca tcattataca
     7801 agcgacaaag attgtcagga agccataact ctccttcttt atttttttca agaataaaca
     7861 cctcacaaaa aaattggaca aaaacataag aaaacgtttc ctgataagtt gcccattaat
     7921 aacatcctaa cttagaatat ctcaaaaaac aacgtgtaga cagaaaaagt acaaaattag
     7981 caagaaatgg ttgtttgtat tgttagaatt tttagaaaat tataccttta gtttctttgc
     8041 ataaaatgat acaatgattg ttaaaattgt gaaagggtgg tcatttgtta gagaagtttt
     8101 tggatatgtc gttagttcaa cgtattgttg ttggtatttt agtcggaata ttgtttggta
     8161 tctttttccc cacatggaca tttatttcaa tattgggaac gttgtttgtc agttgcttga
     8221 aggcaatcgc acctattctg gtttttttct tgacgatggc gtcaattgcc aagcataaaa
     8281 ttgggaataa gacattcgtg aagcctattt tgatcttata tttagtggga acattgcttt
     8341 ctgcacttgt agcagttatt gtaagttcaa tttttactgt tcctattact ttacaagaaa
     8401 cagtatctga aaaagcaccg caaagtttgg aaagtgtttt atctactatg ttgacgaatg
     8461 tcacacaaaa tcctgttcaa tctcttattg atgcgaatta ccttggcgtc ttattctggg
     8521 ctgtattgtt gggactagct tttcgagcac gttcagaatc gacaaaagag ttggtggatc
     8581 agatatctat agcgttgtca caagtcgtac aagtgattat tgcatttgct ccgataggga
     8641 ttttaggatt ggtttatcaa tcaattgcta cgactggaat cgctggattg gcagagtatc
     8701 tgcagctttt acttgttttg atcggtacga tgctgtttgt cgcattagtt gtgtacccat
     8761 tcctcacgtt tttgtttatt aaagaaaatc cctatccatt gattttcttt tgtttgaaag
     8821 aaagcgcact tcccgctttc tttacccgaa gttctgccgc aaatatcccg ataaacatga
     8881 tgttggctga aagattgaaa ttgacaaaag agtcttattc catatcgatc ccgttaggtg
     8941 ctacgatcaa tatgggcggt gccgcgatta ccatttcctt gatgacgttg actgctgtac
     9001 attctttagg gattgaagca ccttttgttt tgaaagtcct tttatgtatt ttgtctgctt
     9061 tagctgcttg tggagcttca ggtgttgcgg gcggttcact gttattgatt ccattagcat
     9121 gtagtttgtt tggtatctct aatgatgtcg cgatgcaaat cgtcggtatt ggatttatta
     9181 tcggtgttat ccaggattca atggaaactg ccttaaattc gtcaagtgat ctgttgttta
     9241 ctgcaatagg agagctaggc gcgcgtaaaa gaagtgggga aaaaatcaaa ttgaaagatt
     9301 gtttagatgg attaagtgaa cgttgaacgt tagatattta gtatttttaa aatgattcga
     9361 gtttttaatg agttatatag atgttcgtgt aaatagaggt ttctgttaaa tcaagataaa
     9421 gtcgtttata ataacaatta aaagcatagg taattgttat tggaggcgta tcatggattt
     9481 tttacgtaaa tttggttttt tctttttatt attaggcgtg ctgagtgatt ttttgactcc
     9541 gtacgtatta ggattgtatt atcctgaatt agatcaaatg aagagcgtga tcagtgtatt
     9601 tggagaggtt tccagtcctg taagaaaagc gtttcttatt tggtctgtgg tttcagggat
     9661 ctttttcatc ctgtcattgc ctgcattgta tgctgcattt gttagtacat ctcgtgtgtt
     9721 agctacccta gttacagcag ccatcgcgtt ctatggtata ggtgattgta tctttacagg
     9781 tttgttcagt atcaataccc atgaatcttc gtggaatgtc tcgacatggg tccataatat
     9841 cggatcaggt ttaggttaca gtggtttttt attgtttcca gttcttcttt tcttgatcta
     9901 tcgaaaaaat gggcaaatgg acgtaagcca cttatacctt ggactgtctg tgatcagttt
     9961 gctttttgcg gcgatttatg gactggcaag aattccttct ataaattctt ggccattact
    10021 gaataaaata ggtttttgcc aaagaatcag ttttttcttt aattatgtgc ccattgcctg
    10081 gttgagtatc ttgcatctca aacagtaaaa cttgagccgt caaattgaac agtcagcgga
    10141 tacttcatcc ggtggctgtt ttttgttttt ctgaaatata tatttccaca gttttcttgt
    10201 atagcttctg tcttagagaa gcaaggagct atattttgtg ctacaatctt aaaaaggtag
    10261 agagaagacc aaatggaaga cagaggagcc actaaaatat gagaaacatc aagttgacaa
    10321 tcgaatatga cggaaaaaga tattcaggat ggcaaagact tggagatgac gataaaacca
    10381 ttcaaggaaa aatcgaaaaa gtattgcatc agatgacaaa tgaagagata gaaattattg
    10441 gatcaggaag aacagatgct ggcacccatg cgcgaggaca agtagcaaat tttaaaacaa
    10501 acacagaaat tggactggca gaaatgatcg actttatgaa tcgttacctt ccgcgggata
    10561 ttgtcatcaa aagaatcgaa gaaatgcctg agcgcttcca ttcaagatat aatgcggtgg
    10621 gcaaaaaata cagctattat gtttggaaca acgtgattcc ttcagcattt gagcgaaacc
    10681 acagctttta tttccctcaa gagttggata tggacaagct gaatgcagct tgcgaaaaat
    10741 tagtgggtac acatgatttc attggttttt ctgcattgaa aaaatcaaaa aaatccacag
    10801 tgcgtacgat tgatgaatta tctatcgaac gaaagggaga aatgcttcat tttacttttg
    10861 tcggagacgg attcttatat aaaatggtgc ggattatcat ggggacatta ctagaaatcg
    10921 gcacaggtgc catgccgata gaaacaatcg atgaaatatt tgaaagcaaa gtgcggcaac
    10981 atgcaggaga aaccgtccct gcacaaggtc tgtttttgga tgaagtttat tatcgataaa
    11041 aatactttga taatggatga aacgcatgtt tcacagtata aatatcgatg ttaaataatg
    11101 tatgaagcaa attaaagaaa tataaaaaaa gactttttag cctgtttatg tgtacagcta
    11161 aaaagtcttt cttaaataaa aatctagtga gaatgttgta cttctcttct cggttgtttt
    11221 tgtttagaaa aagtaaatcg ataaagtaaa aataaaatca aagtaacgat agataaatat
    11281 cttgaagcaa ttgctagttt tgtgtactga taagtaataa cgactttatt tatacctgga
    11341 ggaatagtta attcagttgt gccaaactta gataattttg tttgtactag tttgccatta
    11401 atagaagcca cttgcccttt ataacgataa ataggtgtcg tcaagacagt atttttatta
    11461 ttcgaattat ctaattcaat tgtaagtttg ttactcataa aatgagttgt tggatttatt
    11521 tcctggttac ctagtaaata ttgatcatta ttaatcatat caggatgata gattgactct
    11581 ttgtttgcat aatcagtatg attgtaattt tcagctatag caacagcgtc ttcaggtgct
    11641 ataagtgtta atgaatcttt gttagcatgg atagaattgc ttactcctga gagatgcaat
    11701 attagtgcaa gaaacgtaaa gaccattatt ttccatgaat tatttttagt agaaaaaata
    11761 taatagatca ctatactcat cgtataggct ataaataaag aagaaaaacc atttaaccgc
    11821 cagacaaatt gtagactctt aactggtgta tggttaaaaa gttgccaagg aaataaattg
    11881 gttgaacaaa aaagaacaaa tacaccaaga aagaaaataa ataaatcatc agcagttaat
    11941 ttttttataa aaataaaagc accaatcaat ccgagaaata agaataatcc aatagtatag
    12001 ttgttatagt tattatttaa aatatgtgtc agcatatcac taggagccat accacttaat
    12061 tcttttccca gtgggacttt caattcttgt gctctagatt gttgaataaa aggaattaag
    12121 aatcccgcac ttaaaaaaaa ggtgaggacg gtagctttta aaagacttaa gagtctagcg
    12181 attttttggt cccaaaaata aaaagaaaga aacaacgttc ctccaataaa tacagaaacc
    12241 atagcgacag aaagtaaatg agtatacact actagcgtca tgccaataga caaccagtac
    12301 catttttggt aatctctaat ataaatttcg taacacccca ttagtatcaa aggcaaaaat
    12361 gttaaagcga cagcttctcc caatgaagca cgatggaata tgtcaatggc acgataggcg
    12421 gaaaatgtat aaataatcga aaataacagc gaaatataac gattgttttt tatttgtttc
    12481 atagaaaaat aagaaacgac cattgttaaa aaagtgataa aaaaatagta gatattataa
    12541 cttaaaacaa gatttcccat cattttataa aataaaaaag ctggataaag agtaagccat
    12601 ggataaaaaa tattcatcat tgttccatga tgatcgaagt tattgaagtt tactggagaa
    12661 ctccatacat tatctagtcc tatgattcga gataaatgaa agtaagtatc ctgattgaca
    12721 ctatgataga ttcccttaga catgaataga gggatgacgt aagcaaaact tagaaataac
    12781 ataaaaatga tatctattat ttggttttt
//
LOCUS       DAZKMB010000080        12746 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000080 DAZKMB010000000
VERSION     DAZKMB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.080"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8V3_001989"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8V3_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0300511.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8V3_001990"
     CDS             complement(323..892)
                     /locus_tag="ACG8V3_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0300512.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8V3_001991"
     CDS             1078..1980
                     /locus_tag="ACG8V3_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0300513.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8V3_001992"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8V3_001992"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0300514.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8V3_001993"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8V3_001993"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0300515.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8V3_001994"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8V3_001994"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0300516.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACG8V3_001995"
     CDS             8554..8739
                     /locus_tag="ACG8V3_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0300517.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACG8V3_001996"
     CDS             complement(8801..9997)
                     /locus_tag="ACG8V3_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0300518.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACG8V3_001997"
     CDS             complement(10167..11603)
                     /locus_tag="ACG8V3_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0300519.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACG8V3_001998"
     CDS             11896..12705
                     /locus_tag="ACG8V3_001998"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0300520.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4017 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aatttt
//
LOCUS       DAZKMB010000081        12742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000081 DAZKMB010000000
VERSION     DAZKMB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.081"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..544
                     /locus_tag="ACG8V3_001999"
     CDS             101..544
                     /locus_tag="ACG8V3_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0300521.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="ACG8V3_002000"
     CDS             complement(550..1734)
                     /locus_tag="ACG8V3_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0300522.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="ACG8V3_002001"
     CDS             2002..2700
                     /locus_tag="ACG8V3_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0300523.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="ACG8V3_002002"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="ACG8V3_002002"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0300524.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="ACG8V3_002003"
     CDS             complement(3510..4043)
                     /locus_tag="ACG8V3_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300525.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="ACG8V3_002004"
     CDS             complement(4340..6721)
                     /locus_tag="ACG8V3_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0300526.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="ACG8V3_002005"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="ACG8V3_002005"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0300527.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="ACG8V3_002006"
     CDS             7422..7961
                     /locus_tag="ACG8V3_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0300528.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="ACG8V3_002007"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="ACG8V3_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0300529.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="ACG8V3_002008"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8V3_002008"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="ACG8V3_002009"
     CDS             9068..10234
                     /locus_tag="ACG8V3_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0300530.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="ACG8V3_002010"
     CDS             10280..11776
                     /locus_tag="ACG8V3_002010"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0300531.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="ACG8V3_002011"
     CDS             11857..12540
                     /locus_tag="ACG8V3_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0300532.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12549..>12742
                     /locus_tag="ACG8V3_002012"
     CDS             12549..>12742
                     /locus_tag="ACG8V3_002012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300533.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4273 a         2230 c         2630 g         3609 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggc gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta tt
//
LOCUS       DAZKMB010000082        12448 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000082 DAZKMB010000000
VERSION     DAZKMB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.082"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..1340
                     /locus_tag="ACG8V3_002013"
     CDS             39..1340
                     /locus_tag="ACG8V3_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0300534.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1519..1788)
                     /locus_tag="ACG8V3_002014"
     CDS             complement(1519..1788)
                     /locus_tag="ACG8V3_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0300535.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(1778..2011)
                     /gene="relB"
                     /locus_tag="ACG8V3_002015"
     CDS             complement(1778..2011)
                     /gene="relB"
                     /locus_tag="ACG8V3_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0300536.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            complement(2113..2337)
                     /locus_tag="ACG8V3_002016"
     CDS             complement(2113..2337)
                     /locus_tag="ACG8V3_002016"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0300537.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            complement(2545..3177)
                     /locus_tag="ACG8V3_002017"
     CDS             complement(2545..3177)
                     /locus_tag="ACG8V3_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0300538.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            complement(3190..5913)
                     /locus_tag="ACG8V3_002018"
     CDS             complement(3190..5913)
                     /locus_tag="ACG8V3_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0300539.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            complement(6049..7014)
                     /locus_tag="ACG8V3_002019"
     CDS             complement(6049..7014)
                     /locus_tag="ACG8V3_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0300540.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            complement(7068..7880)
                     /locus_tag="ACG8V3_002020"
     CDS             complement(7068..7880)
                     /locus_tag="ACG8V3_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0300541.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            complement(7894..8667)
                     /locus_tag="ACG8V3_002021"
     CDS             complement(7894..8667)
                     /locus_tag="ACG8V3_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0300542.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            complement(8681..9151)
                     /locus_tag="ACG8V3_002022"
     CDS             complement(8681..9151)
                     /locus_tag="ACG8V3_002022"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0300543.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            complement(9148..9564)
                     /locus_tag="ACG8V3_002023"
     CDS             complement(9148..9564)
                     /locus_tag="ACG8V3_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0300544.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            9907..10731
                     /locus_tag="ACG8V3_002024"
     CDS             9907..10731
                     /locus_tag="ACG8V3_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0300545.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            11126..11743
                     /locus_tag="ACG8V3_002025"
     CDS             11126..11743
                     /locus_tag="ACG8V3_002025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300546.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            11858..12136
                     /locus_tag="ACG8V3_002026"
     CDS             11858..12136
                     /locus_tag="ACG8V3_002026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300547.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(12190..>12448)
                     /locus_tag="ACG8V3_002027"
     CDS             complement(12190..>12448)
                     /locus_tag="ACG8V3_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0300548.1"
                     /translation="GITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFFSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         3916 a         2440 c         2019 g         4073 t
ORIGIN      
        1 aattgaatca ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca
       61 aactactact tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca
      121 ttaagaatat caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc
      181 ctttgtgtaa acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc
      241 tcattcaact taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct
      301 accactgtcg tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact
      361 gctttatcgc taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa
      421 tgacgacaat tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt
      481 ctctgatccc catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg
      541 aatttcgttc actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata
      601 ctggaaaatt actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta
      661 tgcaatttga acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt
      721 cttatgcctc tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc
      781 acattgtcaa acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct
      841 ttgatcgaaa cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc
      901 ttctgatcaa acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg
      961 gttgggcgta tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat
     1021 tacgaatcgc ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg
     1081 acactttctt taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta
     1141 taaaaaaagc ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt
     1201 caaatgccaa aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg
     1261 gctttcgaag ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct
     1321 atgaatcaaa aaacatctaa agtagaaaag gaattctacc ttagatgttc ttatctcact
     1381 ctatcagtcc tatttgacaa agagccataa aaaagcagaa accaaccgat agtatcgggt
     1441 agtttctacc tgtggtatta tgcaaaattc aaaagattat acattgactt tcgaaactct
     1501 acttcgttcc aaaaacggtt atcttttata tattgtactt ctatgtccaa ttgagaatat
     1561 ctggatttca atttcagaat cacaaatatt agccaatatt cgatagtttc caattcgata
     1621 gcgccattcc ccactcttat ttccagtcaa accttttccg tgctgacgcg ggtcagtcgt
     1681 gttttctaaa ttctttctga tccaggcgac aattatttta gcttggtttc tgtccatttt
     1741 ttttaattgc ttctgggctt gcggcatgta gatgacttta taattcattt agcagtttct
     1801 ccatttcctc ttgagaaata ccactctctt ttttctcttt ggaaacttca attgcactcc
     1861 gtaaagtccg tagatcgatt tcatcttcga ttttatccag tacagcattt cgataaagtt
     1921 cggtcaatga agaattgtgt gcttttgcat attctaatac cagttctttt tcatcgtcgg
     1981 gaatcctaaa tgtaatggtt gattgagaca tgattctacc tccgttcatc atttgtacta
     2041 acattgtact aaataaaatc ctttttgtca atacaacgtt agtacaaaca agatttgcat
     2101 catatccatt attcaaattc tttgagatag cacggtatcg caaaatctct gactgatcgt
     2161 tttaagaagc tggtcatttt gaagcgattt tctccaattt tcagggatca cttcaccccc
     2221 gtaaagaata cccgcaagag caccagttac ggcagcagta gtatcagtat cccctcctaa
     2281 attgaccgca gtcaatatgg ctgcttgata actattcgta ttttggttct gttgcaaaat
     2341 attctagcaa tccctaggat cattcacatg accttaggga ttgcgaacaa aataaaaaac
     2401 ttaaaaagcc aatcccatta tcactgccga agcattttcg taaaaggtgt taccagcacc
     2461 ttttactaca cctgaattat agcaagtttt tttggttagt caaaatagct agcacagata
     2521 tatagcctta tttccattaa ttcactaacc tttttctaaa acaatttgtt ccagttcttg
     2581 tagactttca atttgtatgt ctgcttcact ctgatcgatt tgcattggtt gttttagtgc
     2641 cgccgtatag atatttgctg cctttgctga tttaatacca atcgttgaat cctctactgc
     2701 aataaagaag tcacagtcta attgctgttt cgcttttaaa tatatttcgg gatgtggttt
     2761 gctttgtttt aatgactcac cacttataac aaaggaaaaa tattcagtaa gttcacattg
     2821 aatgagcata ctctctatct cgacagctgg agaagaagat gccagtgcca atggtatatc
     2881 ccgcttcttg aggaagtcaa gtaacggttt cacttctttt cgtaaagctt tagcgaaatc
     2941 aattggatgc gtttttcgat aatctacata tttcggataa agttcctctc ttaatgattt
     3001 gtcctcagga actaatgttt cccatattgc agcttctgtt ttccccacat aatcatcaat
     3061 ctggtttgat cctggatcta tctccagttc ttgaaagaag ttcattcttc tctcaaaata
     3121 aaatgtctcg ctatctatga gtaccccatc catatcaaaa attactccca ttgtcattcg
     3181 aattcactcc tatccttgaa caatatcgct caataatcgc aactctaaat ctggtatttc
     3241 aattgtgtat tccctttcaa tctcaaaaaa agcgctatga attttatcca ttatttctcc
     3301 atttgcttgc atatattctt caatgtcctc ttgctcatcc acactttctt tacgtacaat
     3361 tcgttcaatc atacaactac agtgaaggta caacaaaaac agctgattat tagaaaaatc
     3421 aaaatttaat aacttcgata attctttaat tgctttttgt acaaaagata gcaccttatc
     3481 tggatccaaa atggttaaat tttcgacaat tttactaatt gaaaaattga aaattaattc
     3541 tttttgtaaa tcgttcactt caatagtcag ctctttttcc aagtaatgaa caaattcgcc
     3601 aatgcttccc ccattaatca ggcgatctaa tccaatatat ggaatctgat taattttggg
     3661 atcttctgta cctacaatca tgatgacatc gtataattga aaaggagtct caaaattctt
     3721 gttttctaac agctttttgt aatcatacgg aataattact aattctctaa ctgcatcttt
     3781 caagcatttc gttaaaatct cctgaatctg ggtcgcacta ccaatccctg tataacaaca
     3841 agtaatgatg gctttctttt tctttttctt gggaagtagt aactgctttt tgaaagtaag
     3901 acttgtttca atttctgctc ctatttcggt gatactttta ttgtttaata cttgttggcc
     3961 gacttccaat aataacggtg tattgatatg atcaatgatt aacagtggtc catttagatg
     4021 attcactaat aaaccgccta gttggtttag tgaacccata tctaccaata aaatcaagcc
     4081 tgaatctgtt cgataatctc caaggaattg aatcaatttt gcttcaattt tctctaatgt
     4141 aatatctaaa ggcatatcat aagcttgaaa aacgttctta ttcagcatac ggttggcaac
     4201 attcgccata ctactagctg tagaataacc atgagctagc acaatactct taatacctgt
     4261 tttaacattc gtgacattca aactataaaa atatagcgta ataaagaaaa tgtcctcttc
     4321 atacacaggt tgatctaaac actcagataa aaggcttaag attttttttg cataccaata
     4381 aggggtttca agaacattag caaaaaattc gttaagctga tctttggttt tcttttcttg
     4441 aagactattt tcaaaaatca cttgattatc cttgacatat agatagttgg ccaaagcgac
     4501 tacccggtta ccatcttgtt caaacccgta gctttctcgc ataaaatcaa tactttgacg
     4561 aatatgatag tttagaatgg cgtataaaga ctgctctttt tcgtaatcct cttgaaaaat
     4621 aagcgtatcc attacttgtg ttaccttggt aaccatttct tctaaaaata gtttaatatc
     4681 cttgttattt tcaatcactt cagagaatcc agtaagtaat tggtcaaaaa attgaacaaa
     4741 aagctgattg tttctttcta gttgcacttg tcgtgtttct tctggatagt agattcgatc
     4801 tgctcttttc tttctgggtt tatttagttg ctccttgaat ttataactgt actctaaagg
     4861 catatccaat aatttaactt gaattgtttg ctgactcttc tggtggatat atgcgtttcc
     4921 gcaagcatac ttgataatat ttttaatatt ccccacgtta ccttcaaaat cactattcat
     4981 taagaaatga atcaatgcat cagaaacaga tagttcctta gctaaaattt ggctctcaga
     5041 aacaaaaaat tcatcaatca atgacagacg ttcaaacgac ggacgttgtt gaaatctagg
     5101 tagattaaca ataactggca atctcctaag aaaagttggt aagaaagtcg tatatatatt
     5161 ttctgttgtt gcaaagacta atcgaacatc ggcttttttc cgaatactat tatcccccat
     5221 aggagagaat tcacccgtat ccataaatgt aaataatttc tcttgccctt cttcagataa
     5281 gcgatgcact tcatctaaaa acaatactcc tccatcggct ttttctagca aaccagtatg
     5341 ctcctggctt gctccggtaa atgctccttt cgtgtagcca aataaagcag aggatagtaa
     5401 ctctggattg ctcacatatt gtgcacaatt ataatctatg aaaggtgccg atgtgtgaat
     5461 gaccttttct tctactgcat attcatatat tttattcgct agaaaggttt tccctgatcc
     5521 actagcgccg atcaataata aaggcaaccc ttttccagga tataagactg ccgttttaat
     5581 ttgatcaatt gcttctttca aacttccttt agagccaatt acttgatcta atgcgggttt
     5641 ttgagaaatt gttaacaagc tttcaattga ttctatttcg gagttattca gtgtgatatg
     5701 tagttcttca agagctgttc tatgtaaaaa taaaaccggg taacttttta cttttatgaa
     5761 aagcccttca cgaacacctt catttaagta gctactgatt gtacttctat tcaactgtag
     5821 agcttgcgaa atactgcgag ctgaataatt atctgaaggc gtagatttca aaagcaatgt
     5881 tagttgataa agatagtcat ttaatcgctg catctgccac ctgctttcac aatatgtact
     5941 agttatttta ccatatgtac tagttatttt accatatgta taaaatcaca ttgaagaaaa
     6001 caaggtggct taatttctca agccacctta tttagtttga tctatttttt aataagttag
     6061 tttccacata taacgtctct tagttaatgg atgttctctt gcttctgaca attcttctgc
     6121 ataaactcta aatactgcag taatcacgaa tgggttaaag tagtctaaga cttctttagc
     6181 aatatgtgaa gacaagccat aatccaaggc atctacaact tctgttttcg tatcaaagcg
     6241 ttcaaggaaa gttaatgctc gagcatctat atttctagtt tttccatcat tcattaataa
     6301 tataaacggt acgtctttat ccacaatttc gaatggtccg tggaaaaatt ctcctgaatg
     6361 gaaggtacca gaattaatcc attgcatttc catcatcaag caaattgatg ttgagtatgc
     6421 tacttcatgt gtagcaccac tactcattac ataaataact ttttcatctt taaagcgttc
     6481 agcaaatttc ttcgcatctt tacgagctga ttctgcactt ttttgggcaa gatcatagat
     6541 agtagcaaat ccttcttgca ttgcttcata atggtcataa ccttctactt gatgtaaaag
     6601 ttctactgcc aattctaaac agtagcctgt tttttctaat ttagcagcat agttctctgc
     6661 aaatcgatga taaattacat aatctgcatc ttttgttaac ggtgagtcaa tgcttcgtgt
     6721 tagactaata actgtagcac ctttttcttt agctactgcg ttagcttcaa ctgtttctgg
     6781 agtcgtacct cctaaagaag ctgaaataac gacagttgac tcatctacag atgctggtgt
     6841 tgcatagtta aattcgctgg ctgtgtaatg actaatccgt aactgcttcg cattttggtc
     6901 tagaaaatac tttgcaggat aaaggtccgc ttttgaagcg ccacagccaa caaatacgat
     6961 tgattttacc tcttttttcg ctacgatttc tttaacaatt tcttttgaat tcatcaattg
     7021 aattcctcct tatttatatt attcaaccaa atttattggt tattccctta ttgtaagata
     7081 cctaacaaac taaaaataat tcctccgact aaacagattg ccaacatcca tcctgttttg
     7141 acctttttct gcaataacca gtacatgaag aaggttaatc ctaatggtaa aaattgtggc
     7201 ataatactat ctaatacatc ttgtaagaca aaagctttac ccatttgaat tggtgttgta
     7261 atcccgatca tgctggcaat catcgaacca atgaccatca accccacaat ggagcaaaca
     7321 tacattactt tatccatcaa accttcttga tacagtttag acaagtattt attccccatg
     7381 ttatagccaa ttttcccacc atagtaagta actaaaaagg aaggtacaaa ggaaattaac
     7441 attgctagaa ttggacctaa aatactacct tgttgggcta aattaattcc tagtccaaag
     7501 gctaataccc gaatcgttcc ttgaaagaaa gaatcaccaa caccagatag cggtcccatc
     7561 agagaagtct tcactgcact gattgattcc ggattaaagt tttcttggtc ttcgtagtat
     7621 tgctcctcca tagaagcagt taaccctaac acaaatgcac ttgtttgagg ggtacagttg
     7681 tagtacatca tatgccgctc gtaagcttct ttcttcattg cttcatcttc tggattcttg
     7741 taaatgttat ctagaattgg tgcaattccg tacataaaac ccatgttcat ttgtcgttca
     7801 tagttccatg aggcttgaat tgcccatgaa cgccataaga actgccgata tttccatgca
     7861 ttttgatctt tctctttcac ttaatctacc tccttataag tcatctaatg gatctggctc
     7921 attttggtta ccatttgagt ttttaccttg aacttgaacc atgacaaacg caacaataca
     7981 tgcgaaggct gcaataccaa tgattcccaa tccagaataa gcagtaatta aaaatccaag
     8041 taataaaaat ggcaccattt ttttcgttaa catcgtttgt aataacaacg caaacccata
     8101 tgctggtaaa attttacttg caaccgttaa gccatttgtt agccactcag gaatcgcatc
     8161 aacaatctga gaaacaagat tcgttccaaa ataaattgct agaaaaatag gaataaaata
     8221 catgagagca tataatcccg agccataaag tatatgaatt ctccttgctt ggctaaattt
     8281 tccttgttcg ataaagcgat ccgcaacgtg agtcaaattt gacaaaatcg tccgcattaa
     8341 gatacctagt atttgaccta acactgaaat tggaatggca atcgttaatg ctgcttctgc
     8401 attcgcattt gataaaatgg caaaggctgt agcaataatt gatgccatat tcatatcagg
     8461 tggtgaagca gctccgatat ttactaatcc taaggacatc aattccaaag aggctccaac
     8521 aattaatcct ttttccatgt ctccaagtac caaaccaaca agcgtactga caatcaacgg
     8581 tcgttcaaaa ttcattcttc ctaaaagcct tgagtctaaa atacaaaaaa caccgattaa
     8641 ccctaaacca attgactgaa cgagcatttt cattcctcct ttataacatt gtattcaaat
     8701 cttctttttt agaagttggt acctgttgaa taaattgttt aatgccaaga gcttttaatt
     8761 ttttggcatc ttcttgctca gattcattca agaaaatggc attactaaat tgcttggaac
     8821 cttctttttt gattatccct ccatagttga tttcttgtac tttaggatag gcttcacaaa
     8881 attgacgaat ttcttttgta ttaccaaaaa taagcatgat attttcgtta agtgtttcga
     8941 ttttaggcat tttttctagt aactgttcaa ctgaaaaaat tgttaattta atgccagtcg
     9001 gtttagataa attcaaagac atttttttga attcatcatt cgctgcctct ttattggcaa
     9061 taataatacg attgatattt aatgatttac tccaagaaaa tactacctgt ccgtgcaaca
     9121 aacgatggtc aatacgtact aacttaatca taggtcatct cctccagtaa gttgttctac
     9181 taataatttt ctacaatcaa tcactgattg ttgactctct ttaataagct tttctagctc
     9241 actttcccca atttttccat tataggttgc aatagaaata actaaaggta agttcattcc
     9301 agtaattaat tgcaatccct catactcttt aataatagtg agcatatcat tattcacact
     9361 accacctaga atatcagtta aaatgtaggt ttcttctata tctattgtgg ctaaaagttt
     9421 ttttatttgt accaaaatag attcatcttc atctctaaat gctgacaagg catgaattgg
     9481 tacactatct ccaataatca ttgtcgctgt ttgctttaat ccttgagcaa attccccatg
     9541 gctggcaaga attatagttg ccattttcat tcctcctatc cttacgcttt tctataagca
     9601 agatgcgtgc caattttagg aaacgctgtt aattaaaggt ttttctatat catgccaaca
     9661 cttttctttc cctaaattat agtgttgatg aaagtgtggg tatttaaagg tgtctaatta
     9721 aatgatcact gtaattggaa tttagctttg ccccaatccc ccgccacatc ctaagaaatt
     9781 ttcttccaca tacgaaataa agcgtaaaaa gcgtccatta atattttttt cggtaattac
     9841 aacaaaaaat agaacttaat gtgctacaat atatgaatac acgttcatgt gaaaggagat
     9901 gttatagtgg aaatcaaggt tttatttgaa aactcaagaa taaatttgaa ctttaaaagt
     9961 ggacatgggt tatctctctt aattaactac tcagggaaga aatatctctt cgatgctggc
    10021 gctaataaaa aattagtcta taatgcgact aaaatggggg aaactttgaa tgatctggag
    10081 gcagtgatta tttcacacgg tcattctgac cacaccggcg ggttaagata tttaaaatca
    10141 ggaaacgccc ctatttatac agctgaaggt gtaactttac cccattattt aaagatttta
    10201 accacgtttc tgtatgttgg tattcctaaa aatgaaatcc ttaaaatgca tgagaattta
    10261 catacaataa aaacgaaaac agaacttgct ccgagcttcc atatcatccc cctaggcaaa
    10321 gcaaactcga ccaccaagaa tttatacaaa aagatggatg ataaatttgt ttatgatgat
    10381 ttttcagatg aattattggt cgtaattgag caagaagacg gtctgattgt ctttactggc
    10441 tgtagtcatc atggcattat cgatatgatg gaaattgctc ttgaaacatt tccgactaaa
    10501 cccataaaat acctaattgg tggcttccat atgattggca taccttatat aaataattta
    10561 ggtctacctg agcaagaggt aatgaaaatt ggaaataaat tgaatgactc acgagtagaa
    10621 aaaatcctta cttgccactg cacaggttca aaagccttca acatattaaa aacacagtta
    10681 ggaaacaagc tagaagctat taaaactgga cagcacttag agataagtta aaagagaaac
    10741 tgataggact gtcgagagga caaaagctat atgaaatacc ccctctaatg tttgctggcg
    10801 ataacacaaa aggcagacct tctgtgaata gggctacctt tttgtgtttt ttcactaact
    10861 ttcaatatct tggtcattca aggaaaatca cctatatttc tgtgtgggtt tacttcctta
    10921 ttaaatcgag tcatctattt cgtttattca ctttttcaag tggacattca ttaagctgtt
    10981 tatggcagaa taaaagtaca tacaagtgca acgaaaaact acacaactcc gcaaatcgat
    11041 taagttaaga taaaaagcca ttgcgggcaa cctgaaactc ctctaaagtt atagtctcga
    11101 cactgtaact ggaggtaaga aaggtatgct cacaatgacc gatattaata ctatcagaaa
    11161 tttacgaaat aatcatgata aatccattaa tcatattgca aaggagctag atgtaaattg
    11221 gcgcacggct aaaaaatatg cagatgatga tttgctgccg caacctaaag cacgcaaaaa
    11281 gactggtatg atgtatacag aaaagtgggg aggcatcgtt tccctatggc tttcagaaga
    11341 tgctaagctg ccaaataaaa agcgtaggac tgctgaagca atgactactg ctttaaaagc
    11401 cgatggcttt ccaggatcct accgaacagt ctgtgtattc attcaagatt ggaaagcgac
    11461 acactacttg gaaacactgg aagaccaagg ctttgagcgt cttgaacacc cgccagcaga
    11521 agcgcaactt gattttggta cgatggaagt ttgtcacgat ggagcgttta aagatgtgaa
    11581 ggtattagtg atgactttcc cttttagtaa tgcaggattc gccgttgtac tccccgccga
    11641 gaatcaagaa tgtttgttgg aaggcatgaa agaactcttc agccaatcag gtggcgttcc
    11701 tcggaaaata cggattgata atatgtcaac ggccgttact tagacaaaaa caaagactga
    11761 acaagcggtg ctgactgacg gtttcctccg attttctatg cactatggtt ttgagacaca
    11821 ggtgtgcaat ccacgtagcg gaaacgagaa aggtagtatg gaaaacaaag tcggctatgt
    11881 acggtacaac ttcttttcat ctacaccgat tatgaaggat ttcgccagtt tcaacgaggc
    11941 attagcggtg gagctgtcac acgatcgaga gcgaatccat tacgaaaaac atgtcattat
    12001 caatgatcta tggaacacag aaaaaacaga tttgctagct ttgccggaca ccgattaccc
    12061 agtatttaaa gaaatcgaag tcaaagccaa taagtacaac gaaattaaac aaaacaaaat
    12121 aacaaattta tcgtagttca atatacttga tagaaaaact atttatttta cccgtggtgc
    12181 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
    12241 acttaataga acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac
    12301 agaacgtgag ttgaaatttt gacaacctaa tttttctaag gaaaaaaaaa cagtctcaac
    12361 tgtttttctt tgttttccaa gtaagctgtg gtctagtttt tctgatttat ccatgttttt
    12421 acgtggcgga acggatatag taatgccc
//
LOCUS       DAZKMB010000083        12200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000083 DAZKMB010000000
VERSION     DAZKMB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.083"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..649)
                     /gene="frr"
                     /locus_tag="ACG8V3_002028"
     CDS             complement(92..649)
                     /gene="frr"
                     /locus_tag="ACG8V3_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0300549.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(652..1374)
                     /gene="pyrH"
                     /locus_tag="ACG8V3_002029"
     CDS             complement(652..1374)
                     /gene="pyrH"
                     /locus_tag="ACG8V3_002029"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0300550.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1510..2391)
                     /gene="tsf"
                     /locus_tag="ACG8V3_002030"
     CDS             complement(1510..2391)
                     /gene="tsf"
                     /locus_tag="ACG8V3_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0300551.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2490..3272)
                     /gene="rpsB"
                     /locus_tag="ACG8V3_002031"
     CDS             complement(2490..3272)
                     /gene="rpsB"
                     /locus_tag="ACG8V3_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0300552.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3631..4110
                     /locus_tag="ACG8V3_002032"
     CDS             3631..4110
                     /locus_tag="ACG8V3_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0300553.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4327..5418
                     /gene="phnW"
                     /locus_tag="ACG8V3_002033"
     CDS             4327..5418
                     /gene="phnW"
                     /locus_tag="ACG8V3_002033"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0300554.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5411..5539
                     /locus_tag="ACG8V3_002034"
     CDS             5411..5539
                     /locus_tag="ACG8V3_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0300555.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5543..6088
                     /locus_tag="ACG8V3_002035"
     CDS             5543..6088
                     /locus_tag="ACG8V3_002035"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0300556.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6542..8233)
                     /gene="argS"
                     /locus_tag="ACG8V3_002036"
     CDS             complement(6542..8233)
                     /gene="argS"
                     /locus_tag="ACG8V3_002036"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0300557.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="ACG8V3_002037"
     CDS             complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="ACG8V3_002037"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0300558.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9715..10734)
                     /gene="argF"
                     /locus_tag="ACG8V3_002038"
     CDS             complement(9715..10734)
                     /gene="argF"
                     /locus_tag="ACG8V3_002038"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0300559.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="ACG8V3_002039"
     CDS             complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="ACG8V3_002039"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0300560.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3341 a         2529 c         2203 g         4127 t
ORIGIN      
        1 taattttttc tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg
       61 aatgatccgc ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat
      121 cagcagcgat cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt
      181 ctaatccgcg caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat
      241 cgatcgcatc acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttca
      301 catctttagc taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga
      361 catttccatc attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat
      421 cttgaagaat gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga
      481 ttgatgccat ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca
      541 aaaggctagc gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg
      601 ctgctttttt cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc
      661 ccttacagtt gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt
      721 taaattgaag acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc
      781 catgacttgt aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc
      841 atttgcatct actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag
      901 gataacatct gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta
      961 cgggttgccg gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc
     1021 tttacggcga atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt
     1081 cggtacaccc acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa
     1141 catacccatg taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg
     1201 ccagatattt ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt
     1261 gatttcttgg atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc
     1321 taaagcttct ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat
     1381 tcctccaata ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact
     1441 ggtttttctt gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat
     1501 tcaacagaat tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt
     1561 ttcgatacct tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc
     1621 aacgtatttt tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat
     1681 ttctgctttg aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt
     1741 gccttcgttc aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg
     1801 aatttgtgat tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct
     1861 tgcaactgtt tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat
     1921 atgcaagtat ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa
     1981 gctgattttt tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt
     2041 atctgttttg attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc
     2101 tgcaatttct tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga
     2161 gtttacttca acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc
     2221 tgcaatgcgg tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac
     2281 tgctttttcc atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac
     2341 gccagtcatt tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc
     2401 ctcctgtgtg tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc
     2461 agcctttgaa acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca
     2521 acatctacga tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact
     2581 acttgatctt caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat
     2641 ttaacggcac gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg
     2701 cagtttgtat caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca
     2761 atacgttctt tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg
     2821 ataccgccta agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt
     2881 ttaggtagaa cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg
     2941 atacgttttt ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg
     3001 aagtattgtc ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta
     3061 cctacgaata atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca
     3121 tctactaatt taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag
     3181 atatatttct tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct
     3241 tctagtaatt gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt
     3301 ttcctcttct tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa
     3361 taagaatgtg gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc
     3421 gtatcgcaag agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa
     3481 aagaattcaa gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa
     3541 taattttagg tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac
     3601 atgtgaaatc tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt
     3661 aaacaagtca ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg
     3721 cattatggcg ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc
     3781 gtgaaggcac ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact
     3841 cctgagacta ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg
     3901 aaagtcgaac gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta
     3961 tttgcatctg ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc
     4021 gatacgatca tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt
     4081 caccatccaa ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg
     4141 tgttttttta ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata
     4201 catcattttt tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata
     4261 ttggatgtta acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga
     4321 aatcatatgt ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat
     4381 gctatgctga tcgatcattg tacttgggat caagaataca aagaaattac acaatggata
     4441 agaaaaaaat tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg
     4501 caaggtagca gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat
     4561 actgtactga tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt
     4621 ctgaaactga actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg
     4681 attacacagc tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag
     4741 actacttccg gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat
     4801 cttttgttta tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa
     4861 ctagggattg attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt
     4921 tcttttgtac ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg
     4981 tcacttgatc tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc
     5041 acttccccta cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa
     5101 ggaggaatcg aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa
     5161 atgacagaga tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct
     5221 tttctttacc ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa
     5281 aatggatttg tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat
     5341 attggcgaga tctatccgga agatattttg gaactgacag caattatcaa agactataag
     5401 gagaacgtta atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg
     5461 ctgtatggct cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa cccaactgac
     5521 tgacaaagaa atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct
     5581 ttccctccct tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa
     5641 agaggtggag gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca
     5701 ttctaccttg aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa
     5761 tattggttcc acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa
     5821 aaaagaaagg tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg
     5881 accgtttcct tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga
     5941 agtaatcaaa gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt
     6001 gacagtaggt gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa
     6061 attagagatg gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc
     6121 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     6181 aagaatccag aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa
     6241 gtaataaaaa cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     6301 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga
     6361 cttttgtcac agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta
     6421 aacggtgttc attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc
     6481 tattttctct tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt
     6541 cttacatctt atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag
     6601 tcactgcata aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg
     6661 tatgagcata gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg
     6721 acggttcgta tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa
     6781 ccacttccca gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc
     6841 ctgctttttc caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt
     6901 ctccttcaaa tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt
     6961 tcaaatcgtg gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt
     7021 ccaaatcagg gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata
     7081 cttcttctaa taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga
     7141 tcaaaccaaa tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga
     7201 cagctttcaa ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt
     7261 tgaagtcgta agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg
     7321 tcgcaccgtc tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga
     7381 tctctgcacc tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat
     7441 ccatcttgtc attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat
     7501 catagatttt attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag
     7561 cctctttatc gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg
     7621 gttctgtttc agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt
     7681 ctgctttaac agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact
     7741 gtgttcccca gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa
     7801 tgaaaccgat cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg
     7861 caatattagg agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc
     7921 cataatgttc tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat
     7981 tcatgaaaaa gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga
     8041 ttttttctgc taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg
     8101 aaaaggctgg aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct
     8161 cgacttgttc cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat
     8221 ctttattatt catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc
     8281 tctagcgacg gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc
     8341 caattgtcct aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa
     8401 cagaattctc caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat
     8461 tcgctagcca agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact
     8521 ttcttgaatt atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaaaaatcca
     8581 gatggcctaa aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa
     8641 agacttaaca aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc
     8701 gatattttct aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat
     8761 agctgcttct actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg
     8821 tttcatttct gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac
     8881 atacacattg tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa
     8941 tttttctgaa gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt
     9001 ttctacaaca ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag
     9061 cgtttcgatg acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct
     9121 ccatccacgt ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt
     9181 ggcttcttct tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc
     9241 attttcatcc acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc
     9301 cttcagtact tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc
     9361 aacacatgta tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat
     9421 caataggtta ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc
     9481 gataaatttc actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact
     9541 tgcatccgta gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat
     9601 ttcattcgtc ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt
     9661 tgtgattttc cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac
     9721 gcgagggata aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg
     9781 attttctgct tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc
     9841 catttcagtg atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg
     9901 gaaagctggc aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat
     9961 ttccatattg atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc
    10021 acccattgat acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac
    10081 atcgtcggta atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac
    10141 gatttcttct tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat
    10201 tgcacctgtg actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac
    10261 agtgatacct tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg
    10321 tgttggatgc cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag
    10381 ttcctctacc attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag
    10441 tactttagct gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag
    10501 atattcagga tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt
    10561 agatgttttt tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc
    10621 acgttttttc aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt
    10681 tgtaaagtct ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa
    10741 cattctcctt tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca
    10801 gtaagactgc cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat
    10861 gcagcgtgga ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat
    10921 gccatagcta cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc
    10981 tggagcgatt gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc
    11041 atctccatta ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt
    11101 ttcgtgtaag ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa
    11161 gacatccatc ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc
    11221 taccattgta aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc
    11281 caacactttt tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc
    11341 tttcgcatta gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc
    11401 gtctccgcct tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt
    11461 tgcaaatctt ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc
    11521 ttcaaaagtc atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt
    11581 aaagtacaag tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc
    11641 atatagactg ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc
    11701 taccatttct tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt
    11761 cgatgcaata tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc
    11821 gtcaatcgct tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa
    11881 tgtcttcgca aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa
    11941 caacagacga tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag
    12001 taaaactgtc tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg
    12061 ttgcctcctt ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa
    12121 ttttgtctca ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa
    12181 cgtttttcta tttttaagcg
//
LOCUS       DAZKMB010000084        12131 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000084 DAZKMB010000000
VERSION     DAZKMB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.084"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(253..2328)
                     /gene="glyS"
                     /locus_tag="ACG8V3_002040"
     CDS             complement(253..2328)
                     /gene="glyS"
                     /locus_tag="ACG8V3_002040"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0300561.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2330..3247)
                     /gene="glyQ"
                     /locus_tag="ACG8V3_002041"
     CDS             complement(2330..3247)
                     /gene="glyQ"
                     /locus_tag="ACG8V3_002041"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0300562.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3633..4451)
                     /gene="recO"
                     /locus_tag="ACG8V3_002042"
     CDS             complement(3633..4451)
                     /gene="recO"
                     /locus_tag="ACG8V3_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0300563.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4594..5493)
                     /gene="era"
                     /locus_tag="ACG8V3_002043"
     CDS             complement(4594..5493)
                     /gene="era"
                     /locus_tag="ACG8V3_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0300564.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5508..5903)
                     /locus_tag="ACG8V3_002044"
     CDS             complement(5508..5903)
                     /locus_tag="ACG8V3_002044"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0300565.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5887..6363)
                     /gene="ybeY"
                     /locus_tag="ACG8V3_002045"
     CDS             complement(5887..6363)
                     /gene="ybeY"
                     /locus_tag="ACG8V3_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0300566.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6392..8584)
                     /locus_tag="ACG8V3_002046"
     CDS             complement(6392..8584)
                     /locus_tag="ACG8V3_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0300567.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8608..9579)
                     /locus_tag="ACG8V3_002047"
     CDS             complement(8608..9579)
                     /locus_tag="ACG8V3_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0300568.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10284..11663
                     /gene="brnQ"
                     /locus_tag="ACG8V3_002048"
     CDS             10284..11663
                     /gene="brnQ"
                     /locus_tag="ACG8V3_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0300569.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11945..>12131
                     /locus_tag="ACG8V3_002049"
     CDS             11945..>12131
                     /locus_tag="ACG8V3_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0300570.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIK"
BASE COUNT         3327 a         2576 c         2205 g         4023 t
ORIGIN      
        1 ccctatataa tcataccaca tacagttttt ttgactaggg aaattcctaa tttaaaagta
       61 aataaaacag taacaagatt ataattaatc gttttcattt ttatttcctc acttttcatg
      121 agcgttttga aaattcgaac aaagaataaa ataacaaaaa ttattaaaat agaattaata
      181 acagctattt ataacaaaaa aaacagagac acagcacgtt tcgtgcatgc tctctgtttt
      241 tcaaacaaaa gactattttg tgatgatttg atccaagctt gctaatgaca aagccatggc
      301 agctatttga cttagttgtg ttagtcggtt cttacgtact gctggttcat cagccatgac
      361 catcgtttga tcaaaataat tttcgatcag tggacgaaga gcgacaagct ggtcgtagtt
      421 ttcgtggatc gtttggtcag caaaacttgc tttcattgct tctactgctt tgtgtaattc
      481 tttctctgcg tcgttttcga acagctcagg gtctacttct gattgttttt caagctgcgc
      541 ttttttcgct aagttcacga cacgtgtcaa agcttccatt gaaggtttga agtcttcatc
      601 tgcaagatgt tgtttgaaca attgtgctgc tgcaaatact tttgtcagat ctttttgttc
      661 cgcattcaaa acagcatcga tgacatcatg acgaatattc ttcgttgtca ataattggcg
      721 cagacggcct ttgataaagt cgatcacttg ttgttgccct tctgataaac caatgccgaa
      781 acgatccgta tctttactga taacatcgct aatcgtttct tgcagaacag acaaaggaaa
      841 ggcccatttt ttcgattcga tgatccgaac gacaccgtat gcttggcgac gtaaggcata
      901 aggatcgttt gacccgcttg gaatcatccc aactgcaaag aaagaaaaga tgctatctaa
      961 tttatctgca atcgctaaaa cagaacctac tgtagactga ggtagctctc cttcactgga
     1021 tataggcatg taatgctcac gaatcgctgt cgctacagca ggtttctcgc ctttcagaag
     1081 agcatatttt tcgcccatga tcccttgtag ttcaggaaat tcccctacca tgttggttac
     1141 tagatcaaat ttatagattt cagcagctcg ttgcaaatcc atcaattctt cctcagatag
     1201 tcctacttgt tcgccaatga tacgggcaat gacttgtacc cgttgcattt tctcatagat
     1261 actaccgatt ttttcatgga aagtaacagt ttttagtttt tcaacgtatt ggtcgattga
     1321 agctttcttg tcttcattat aaaagaattc cgcatcttct aaacgagccg tcaatacttt
     1381 ttgattgcct cgaaccacat cttccaattt tacccgatca ccattacgag cagaaataaa
     1441 atgaggaagc agcatacctt gatcattccg tacctcgaaa taacgttgat gttctttcat
     1501 agaagtgact agtacttctt caggaacaga aagatatttt tcttcgaagt cgccagaaaa
     1561 gacagttgga tattcgacta aattgttgac ttcttctagt aaatcttcgt ctagatcaat
     1621 aacccagtga tttttttctg ctaattcgtt tgcttgtgta acgatcattt cttgtctttt
     1681 ttgcggagac gcaatgacga attgttcatt caatttttct tcatattctt ttgcatgcgc
     1741 aaatgtcaca tcttcgccta agaaacgatg acctcgagag gctctgcctg ttttgacatc
     1801 caagatttcg aatgggatta cttcgtcatc taacaatgcc acgatccagt gaatagggcg
     1861 gatatattca aaatcgtatt ctgcccaatg catcgttaca gggaaagtca aacttttgat
     1921 cacccctgct aagcctgtca atacttcttt tgcttcctta ccatgagtgt atttcgttac
     1981 atagacatat tccacacctt ttaattctct aaaagtgata gcgtctgtcg tcagaccttg
     2041 tccgcggaca aatccttccg ctgctttcgt ccagtttcca tctgcgtcca aagcaatttt
     2101 tttggctggt cctttgactt cttcttcact gtccgcttgt cgttctggaa tatttgttac
     2161 tttgattgct aaacggcgcg gtgtggaaaa tgtttcgatc gtatcataag tcaattggtt
     2221 ttcatctaaa aattttgctg ttttttcttc taattgtttc atgcttggtg tgacaacatg
     2281 ggcaggcatt tcttccagcc cgatttcaag taataagtct tttgccatct tattcagcct
     2341 ccttcacggt ttctggattt gctgtgtttt tattcaacaa agggaatcct aatttttcgc
     2401 gttctgccac aaagattttg gctacagcac gagccatgtt gcggatgcga gaaagataac
     2461 cggcacgctc tgttacggat actgcaccgc gggcgtctaa taagttgaat gtatggctac
     2521 atttcaagat ataatcatac gccggatgaa caagattttc atcgatacag cgtttagctt
     2581 ctttttcaaa acgatcaaag ttgcttagta gcaagtcttg gtcgcttact tcaaaagaat
     2641 atttggaatg ttcatattct ggttgtttga agatctcgcc atatttcacg ccatcagccc
     2701 attctaaatc atagacactt tcaacttctt ggatataaga agcaagacgt tctaatccat
     2761 aagtgatctc agaagttact ggtttacaag gcagtccacc aacttgctgg aagtacgtga
     2821 attgtgtgat ttccattccg tcaagccata cttcccaacc cacaccggca cagcccatgg
     2881 atgggttctc ccagttgtct tcaacaaaac ggatgtcgtg ttctaatgga tcgattccta
     2941 aaaggcgcaa gctttctaaa taaagctctt gaatattctc aggtgaaggt ttcatcacca
     3001 cttggaattg atgatgctga tacaaacggt ttgggttttc accataacgt ccatcagctg
     3061 gtcggcgaga tggctctacg taagcagcat tccatggttc tggtccgatt gcccgtaaaa
     3121 aggtatatgg gctcatcgta cctgctcctt tttctgtgtc ataagcttgc atcagcatgc
     3181 agccgtttga tgaccaaaat ttttgtaatg ttaaaatcat atcttggacg gtcaattttt
     3241 tactcatctg ttttgcccct ttcaatttta aagcgttgct gtcaaataag aatacaggaa
     3301 agaaaacgac aaaaaatccc tatgtctgat tacagacata gggacgaata attcgcggtt
     3361 ccaccctact tccgaataaa gattcggcag cttcatttat cgtactcgca agtgcctctt
     3421 caaaaatcgt ttgggtccgg ctcccactct cccgaactcg ctaaaccacg tgttttttac
     3481 tgatcttggt caatgtacgt attcattttg cagttttctt gtttctataa tagatcctat
     3541 cactgaacga tctatttaat ctatggttaa taatagacgg aactccctgt gtttgtcaaa
     3601 tggttttgat tttttgttgt atgttttatt tactattctt ctttttcagg tgtctctttt
     3661 tctttttcag acatgtcttt ctctgacgtc tttaatacgt gtgcccaaga cttcatttga
     3721 ttgatgaact ttttgctttt caaatggata ccgacatatt catcatacag ctggtcgatg
     3781 agttcacgga ttgcttgttt ggtttcttct ttcagatgga ttccactaat tttatcataa
     3841 gaaatagctg aaaacatccg aacgaaataa acagctcgag gatctgcatg ataacggtga
     3901 tcgtccatcg accaatgtct ttcgcatatc acaccaccat atttggaaga atagtcaaat
     3961 tttccttttg tttccccgca tacgacgcag tgtgtccaga ttggtgcaat tccgaagcgt
     4021 tgcagtattt ggacttcaaa aatattcgtg atgatttctg catcgtctcc ttggtccatc
     4081 cgttgcagtg cttgttgcgt aaactggaac aaatgcgggt cgtactgatg atcttcgatt
     4141 gccgcatcta ctagattcaa taaatatgtg ccatagccgc tcaaaaagat gtcttcttgg
     4201 atgtgtcgaa acgcatggac atctttgaca ctgcttaaaa atgacaaacc ttcacttcga
     4261 aaattcccga tataaactgc ttcagtaaaa gggagcaaag ccggagctaa gggctgattt
     4321 tgccgatgga tctctttgat ataaaacatc attttgccag aagattctgt aaagattttg
     4381 actaatttat ctttttcttt gtaattacgg ctagataaaa tcaagccttt tgattctgca
     4441 agctggctca tattctccct cctaaaaaat aaatctggaa aagcagattc tgccgatcca
     4501 gatcatttat gttgcgatct tccgcagctt ttctcaacac gcggattttt attcagttta
     4561 tttctatacc taatttacaa gaagctcaga atatcagtat tcgtcttttc ggtagccgaa
     4621 gtcttgcaga tagacttttt tgtctcgcca gtctttttga actttgaccc aaagttctag
     4681 aaagactttg tctcctaaaa gattttcgat atcttttctt gctttcgttc ctacttgctt
     4741 cagcattttt ccgcctttgc cgataatgat ccctttttgg ctatctcttt cgacaataat
     4801 cgtagcctgg atatggattt tatcattctc gtttcgcttc atcgaatcaa caacgactgc
     4861 gacagaatga ggcacttcgt ctcgtgttaa aaacaagact ttctcacgga tcaattcaga
     4921 aacgatgaag tattcaggat gatcagtcac ttgatcatct ggaaaatatt gtgggccctc
     4981 tggcatttca ctgactaata catccatcaa acgttcgacg ttgtttcctt ctgtagcaga
     5041 gattggaatc acttgtgcaa aaggcatctg cgttgaataa tcttcgatga ttcccaacaa
     5101 ctcgtctgga tggattttgt cgattttatt gataatcaga taaacaggag aatgcacgtt
     5161 ttttagacgt tcaataataa aatcgtctcc ccgaccgcgt ttttgatcag cactaatcat
     5221 aaataagacc gcgtccactt ctttcaacgc actgtaagct gcttctacca taaaatctcc
     5281 taaacgatgc tttggtttat ggattcctgg tgtatcaata aaaatcaact gagcttctgg
     5341 tgtagtgtat actccttgga ttttatttct agttgtctgt gccttgtcac tcatgatggc
     5401 aattttttga cccacgattc gattcaataa tgtggatttc cctacatttg ggcggccgac
     5461 gattgcgaca aaccctgatt tatgttctgg catatatgtt ctccttttta attaaacaac
     5521 aaatgatata ttttagggac aaataaaatc aacccaacga tgacggtaaa acaagcggtc
     5581 actaaaactg ctccagctgc catatctttg atttttttcc caatcggatg aaaatgaaaa
     5641 tccgtaaaca tgtccacgac attttcaaaa acagtattga tgatctctac tatccatacg
     5701 agaaaaacag ctaaaagcag ccataaccat tctatccgat caagttggaa aatcacgccc
     5761 ataaacaaag cgaggattcc gaacgcaaca tgtttcctca tgttgcgttc ttcctcaaaa
     5821 acagttttga ctccttggac ggcaaattcc acggacatca ggaaatgttt gtttttctct
     5881 acgtttttat cttttaagtc cataggcatc taagatctct ttctgtaaac caaacatttc
     5941 tttctcatcc tctggtgtca tatggtcata cccgttgata tgcaagaatc catgaaccgc
     6001 taaaaagcca agttcccgat catacgaatg gccgtaagct tctgcttgct ctgcagcacg
     6061 ttcagtagag atcatgatgt ctcccagtac acgaggcaat ggattctctc catcttcaaa
     6121 aatcactggg atttcgtctt cgccctcttc ttccaaggca aaactgatga cgtctgtcgg
     6181 tgcatctttt ccacggtaat cacgattgat tacttggatt gctgcattgt ccatgaaagt
     6241 aacagacatt tctgtttctt ctggcaactc taaatatgat gctgcaaatt gcagcaagtc
     6301 gtcgatttct ttcattttct cttctgagac ttggtttgtt tcatccatga aggtaatatc
     6361 cattatacat cgcttctttc tattcttctt gctattcttc ttgttcttct tttatcttct
     6421 caacttccga tttcatttta ggatatttga tccgagaatg gaaggtactg cttaaaccgc
     6481 tgatcaatga tttttctact ttacggattt cttttaacgt caatccactg tcgtccaatt
     6541 gtccatcgga aatacgctct tcaataagat tatggacaaa actttcgatt ttttcgctcg
     6601 ttggatggtc cattgcccgt actgccgctt cacaactgtc agctatattg acgattcctg
     6661 cttcacggct ttgaggtttt ggtccaggat aacggaattg ctcttccgta acttccgggt
     6721 tgcgttcctt tgccttaacg tagaaataac gcatcagcgt ggttccatga tgctgtctac
     6781 aaatatcgat caccatttgc ggcatattat aatcttccaa tattttggca ccatctgtca
     6841 catgtccaaa gataatatgt ttactgtctt ctggcaataa gaaattgtgc ggattttccg
     6901 ctcctgaagg caagttctca acgaaaaagc tggcgtgttt gatcttgccg atatcatgat
     6961 aatagcaagc tactcgagtt agtaaagaac gtccaccgat ttctgctaca gcattagcac
     7021 ttagattggc taccatcatg ctatggtgat acgtacctgg tgcttcttcc agcaactgtt
     7081 tcaataaagg atgattggga ttgctgagtt catttaatac gatcacacta tcatcattga
     7141 ccatcaattc aatatagggg tgcagaccta tcgtcagcaa gaatgaaaac agtgcacctg
     7201 tgaatccgca gactagcgtt aaccaagtcg ttgcatcccc aaagctcatt ccttggtaga
     7261 taatcaaaat gacatccatc atcacaggaa aaataatgac ccacatagca gcggataacc
     7321 cttgttctga gatccgtttg cgtctgacga tcgttgccat gagaccagaa aatacatagg
     7381 acatcaaaat aactgtcaga aagttcgtgc caatcgaatc ataaaaaatg aaaagtgcgg
     7441 atactgcttg aaaaatacca gccatgatcc ctgctcttcg attcaagaaa aagcttaaaa
     7501 caagtggagc aaaagccgcg gggtaaaaca gcggaatata agcagcttgt tctgtttgga
     7561 agagttggaa aaatttcatt agtacgacgc ttactgacat tgccgtcacg tagaaaagta
     7621 cgtattttgt tctttctcct gcattttgga attgaagtga attataaatc agtacaccga
     7681 tttgaagcaa aacagctaaa accatggcaa tgagtgggaa aatagaagtc gtttgattcg
     7741 tcaatcctag taattccaat tttttcatcg ctgaagcatc aatttgattt ccttcgcgaa
     7801 caataatttc tccttggaag atcatcactg gttgtacagc ttctcgtgca gattgtttta
     7861 attcttctgt ctttttctca ttcaatacat cattaactac gatgccttga tccactaagt
     7921 agcgaatcat ttgttgctgc tctgttgtga cattcaaata ttgaatctga tcttctgctt
     7981 cttgtttaaa agcttccaat tcgctttcac gaacttgttt actcatctgt tcgtcaatga
     8041 gcttcaaact ttcatcccta actgtgtgaa tttccgtttc agtcatttca aatatcgtcg
     8101 aataaaaatt gttcggcagt ttttggtaaa acgcaacgtt ttctgcattc acgttttcaa
     8161 aatttttctt cagtgtagca atccgctctt ctgtagtagg tttcgggata ttttcatctt
     8221 ccttcgcctt atctaccttg ccattataag atttgttgat ggtatcattc gtcttatcga
     8281 tcaattcgaa taattgcttg atccgattgt tctggtcatc tgccagatct ttttgataag
     8341 tatattctgg agtcactgct tctgctgcca gttttcttct ttgttcggtt tcttccgtgt
     8401 tctcaatcgt tttattcgca cggatactct cttctgctac ttggccttct ctgaaatcaa
     8461 tggccttttg tttgacacta ctaaatgtaa cgccgcagac aatgataaaa aacaagaaca
     8521 gaaggaaagg caatgctgcc tttccgagtt tttcttgtag tttctgcagt gttttattca
     8581 acatgtgctt atcctcctaa gcaaagctta ttcttgatga agatccgcct tttcataagc
     8641 tttgatgatt tcggcaacta cagggtgccg aacaacgtca cttgcttcaa aattaacaaa
     8701 atcgattttt ttgatttgct ttaatgttcg ttctgcatga acaagaccac ttgtcgtccc
     8761 acgaggcaag tctatctgac ttgtatcccc attgacaatc atttttgatt gataaccgag
     8821 acgggtcaag aacattttca tctgagcgac tgtcgtgttt tgtgcttcgt ccaagataac
     8881 gaatgcatca tccaatgtcc gcccccgcat gtaagctaaa ggtgcgattt cgatcacgcc
     8941 tcgttccatc aatcggttcg tatgatccaa tccaaaaatt tggtataaag catcataaac
     9001 aggccgcaga taaggatcca ctttttcttt cagatccccg ggtagaaagc ctaggttttc
     9061 ccctgcctcc acagcgggcc gcgtcaaaat gattttttgc acttcgcctt tttttagggc
     9121 agcaattgct aatactacgg caagaaacgt tttccctgtt ccggctggcc ctacaccaaa
     9181 tacgatgtca tgtttggcta ccgattcaac gtatttcttt tgaccgctgt tttttacccg
     9241 tataggtttt ccattacggt ctttgacgat ctcttcctca tacatctcaa caaaataatc
     9301 taatgtacct ttttgcccca ttttcaatgc cgtcacaaca tctggcgtac ttacgtggat
     9361 tcctcgtttg atcaattcct gtaatgtccg aatgactgag gcagccaagg aaatctggga
     9421 ttgctcacca atgatctgaa tgatttctcc acgtgtatgg atggttgttt ccgttgtttc
     9481 ctcgatcagt tttatatgct tgtcgtgtgc acctaaaagc atgctgacat catcagcagc
     9541 tgtcaatcta atctctaaag aactagactc ttccgtcaaa aatagacatc cccttttttt
     9601 cgattcattc tttcctattt taccatactc agaagaaact atatagcaaa taagaaaagg
     9661 ttgtaaaaga gtcgcttaac catacgtacc aagaaacgtt aaaaaatagc cgtttccatt
     9721 cgctaaaggt ctcgttttga taaaaagaag ctgagaatgt aagttatatg aaagtggaga
     9781 acaaaatatt tcgtttcatt caacaagtat tctctaggta tttttcctgt ttcaaaaaaa
     9841 gaaaacaaaa tagttaaaat attcttgcat ttcaaaaaaa acaggtgtat tattacgtca
     9901 acaaataaac atacagcaaa gcgtcgagga aaacagtagg ctattcaacg atttattccc
     9961 agtgagtcat gtaagtgaga aatgacaata aatcgaatag acgaacatca tttccgagtc
    10021 aatgtcgaaa acgtagatca ttgtggctgc ctccattatc aggctagcgg ataactttat
    10081 caaaaagcgt ccgtgaatga agaagctttt ttaagcgaaa aaaggtggta ccgttctaga
    10141 atctagagcc cttttcccaa gtggaaaagg gctttttttt attttaaggt ggtggttttc
    10201 atggaatctg atattagtgg cattactaat tgctgcaacg tttatatcgt caatcaaaat
    10261 gataattatt ggaggaaaaa tttatggaag aaacaaaaac ttattcatgg aaacaactat
    10321 tgatggtcag ttcacttatc ttcgggatgt tcttcgggtc aggtaatcta atctttcctg
    10381 tccacttagg tcaaatggct ggcgcaaact gggtcagtgc tggcgtagga tttgctatct
    10441 caggtgctct tttcccatta ctagctatct tagctgtagt agtgacaaaa agtgatggtc
    10501 tgtatgactt agctaaacct gtcggaaaat ggtatgctgc cttgttctta atccttgttc
    10561 atttgacgat cggaccattt ttcggtacac cacggacagc tgctactgct tttgaaatgg
    10621 ctgctaaacc atttttgcct gaaaaatata attcattagg tatgttttta ttctctgctc
    10681 ttttcttttt acttgcttat ttccttactg tccatccaac acggttggtc aaatacgtcg
    10741 gaaagtatct aaactccttg tttcttgcat tattagcaat catttttgtc gtttctttat
    10801 tccatccaat gggaaatcta aatcaatctg ctgcagatac ttatcaaaca aacgctttaa
    10861 caaatggtgt cctagaaggt tataacacgg ttgatgcagt agctttgctt gccttaagtg
    10921 tcacttttgt ccatgcagtc cgtgaccttg gtttcaaaga taaaaaagtc acagaattaa
    10981 cggcaaaagc tggtagcctg agcgttgcgc tagaagtcat gatctatttt ggacttgttc
    11041 ttctcggtgc gatgagtttg aataagatgt ctttatcaga aaatggcgga actgcacttt
    11101 ctcagatcac taatttttat ttaggtaact tcggcgctag cttcttagga gtgatggtca
    11161 cattaggtgt cttcactacc gctatgggac tcgttgtttc ttttgcgcaa gatttccata
    11221 aactgttccc taaagtcagt tatatgacat ggctgcggtt aacgactttc gtctcttttg
    11281 tcgttgcaaa tgctggttta gacaatatca tccaatggtc tttaccagta ttgatgcttt
    11341 tgtatccatt gtcgcttgcg ttgatcttgc tttccttaac agcaaaattt ttccaaaaga
    11401 caccatttgt ctatcaagta acgatgttgt ttgctgctgt tcctgccgta ttggatatgc
    11461 tggcaaattc gcctgcttta gtgagtcaac aaagagttgt agcaagcatg ctcgaatttt
    11521 atcatcatca cgttcctttt gctgcattag gacttggctg gatggtccca acgttacttg
    11581 gttacgctgg cagcctactg ttttattacg cctatcgcct atcagggtat aaacaagaag
    11641 caaatgaact accagaagaa taaccaaatc aaacacaatt taacataaga aatattaaac
    11701 aggaaaaaac cagctagatg aaagaatcct attctttcgt ctagctattt tttagttctc
    11761 ttttttacgc gcacaaaaaa actgcggaca atgattgtcc gcagtttaaa tgggctctat
    11821 aatatctagt gttggtgacg ccttctgttg ccaatacttt ttcttttttc taataaacgc
    11881 aaacagacac cttgaaatcc tttagaataa agtcgacgaa aaccattcaa aggagtgttt
    11941 caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc taaatcttat
    12001 ctttcatgaa gatgcctttc gcaaagagcg aatcaatgac gaaacatgtt ttgtttttga
    12061 tggcacattg acctatcaac cagaggaatg cttccattgt cactatcaaa ataagcaaac
    12121 gatcattaaa t
//
LOCUS       DAZKMB010000085        12021 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000085 DAZKMB010000000
VERSION     DAZKMB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.085"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..962)
                     /locus_tag="ACG8V3_002050"
     CDS             complement(51..962)
                     /locus_tag="ACG8V3_002050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0300571.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1339..1977)
                     /locus_tag="ACG8V3_002051"
     CDS             complement(1339..1977)
                     /locus_tag="ACG8V3_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300572.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1981..3963)
                     /locus_tag="ACG8V3_002052"
                     /pseudo
     CDS             complement(1981..3963)
                     /locus_tag="ACG8V3_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4143..4808
                     /locus_tag="ACG8V3_002053"
     CDS             4143..4808
                     /locus_tag="ACG8V3_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300573.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5205..7238)
                     /gene="brxL"
                     /locus_tag="ACG8V3_002054"
     CDS             complement(5205..7238)
                     /gene="brxL"
                     /locus_tag="ACG8V3_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0300574.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7356..9878)
                     /gene="pglZ"
                     /locus_tag="ACG8V3_002055"
     CDS             complement(7356..9878)
                     /gene="pglZ"
                     /locus_tag="ACG8V3_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0300575.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(9994..>12021)
                     /gene="pglX"
                     /locus_tag="ACG8V3_002056"
     CDS             complement(9994..>12021)
                     /gene="pglX"
                     /locus_tag="ACG8V3_002056"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0300576.1"
                     /translation="KKDLFSVFIKLNSQMLIDNGYASFMTPFVWMFISSYEKLRSFLI
                     NERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFKGGMEVQKIKTLEA
                     IHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTNDVADAKQGLATGN
                     NNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKWYGNNEYVVNWEND
                     GDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQNQAIFETKGSVLFS
                     KDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVTSERKLKKIREKVD
                     ENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLVNQFNTLKSNEEEL
                     NDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCIFGRYSLDEDGLIF
                     AGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVEITFGKETLEENLQ
                     FIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGKQNGFKALMYLHRY
                     TPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTKEIVKIQKQLKEVR
                     EYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKGVKEPKK"
BASE COUNT         3796 a         2306 c         1731 g         4188 t
ORIGIN      
        1 gatgaacagt tttattgatt taaatagttc atatttttta tttataccgc ttatttacta
       61 cttgctgtac tagaatctga agtgaatgta ctcaaaacat tttcaaagtc tttgtctttg
      121 atttttacat tagcgtcttt caatacttcg ccaattactt tattctggaa ggtactgtca
      181 cttaattttg tgtctgttgc aatttctttc aattcttttt tgtatttatc catgtcgttg
      241 cctttgtttt ggtttttcac cattttaacc acataatatt ccgtagtata agttgaagca
      301 ttggtagaag tgatcacatc agaaacttga ccatctttca acttaaaggc tgcttctttg
      361 acttctgccg gaaccgttgt agtagtggaa tcaaatttca cttttccgcc atcttctttt
      421 gtcgtagtat cagttgactt gtctttggct aatttagaga aatcgtctcc cttatcagca
      481 gctttcttca catcttttgc atcatcttca cttgctactt ggataatttg agcttccact
      541 tctggatgga aagattccca agccgttttt aaatcttcat cggtcaattt gatatgtttc
      601 tttagacctt cttgaaaggc tagactttgt ttgatactgt ctttgaatgt ttgttctgtg
      661 taacctgcag atttcaattg tgaatcaaat gaatcaccca attgtttctt tgtttgatca
      721 tattgggaat cgatttcttt ggttgatacg tcatctccat atttatcttc aaatacttga
      781 taaacgatca actgttgcac agcggaatct gctccttgca tttttttcaa gctatccatt
      841 acttcttgtt cgctgattgt accgccttta taactaacgg ctgttttttc accggcattc
      901 gtacagcctg acaataagcc agtgcctagt acgatggcaa gtgctaaaaa tctttttttc
      961 atttatcaat tctcctactt ttcgtttttt gcgaattgct aaaaacaaag gtgttcaaga
     1021 tgaaaatcat caagaacacc tttactaatc tgctcgatac gtcctgaata gatataaggc
     1081 tacacaatcc agaaagttcg ctcagcagaa taaatgtcaa gtagcacatc acaccttaat
     1141 atactacttc ttttgttacc gtttttcaag tctatcttgc gttccgtatt taacgtaact
     1201 aattcttaga acaaatgtta tcgattttgt ttttcttctt ccgctgttgg gatattcaaa
     1261 tcagacactt ttatgttcga ttttccagac atttttttgt tgtttttatt agaaaaagtt
     1321 tgttttttca tttgttttct aaagcatgat cttcttgttt cctattttgg tcagatatgg
     1381 atcatgcgtg accacgataa ctaacgtacc ttgggaagcc tgttgcgata agatttcaac
     1441 gacttgctcc ttatttcgtt tatctaatga tccagttggt tcatcggcta aaatgatttt
     1501 gctattcttc aataagactc tcgcacaggc gattctttgc ttctcaccac cagataatgt
     1561 gtagataggc gtacttagct tcttgtttat atttactttt ttcaatgcct catactttgc
     1621 tgttcgctta tctgaaacat ttaaattcga aaacttcaat ccaatatcta aattaaaatc
     1681 gattgtttca tattcaatca acccaaaatc ttgaaaaaga aaagagaggg catttctttt
     1741 tatcaagcgc ttctctcttc cagttaaatg ttttgtttct tgattaaata aataaaattg
     1801 ccctgaactg aattgatcat tcaatcctaa tatattcaat aaagtacttt tccctgctcc
     1861 gctctctcca actacagtca caaaatcccc agcatccgca gtgaaacgat ccaaatgaag
     1921 aatcgttttg tttcgaatga tttttttcgc accttttatc actatcaacg gctccaattg
     1981 ttaacctcca ttcatcagca ataactgact tttcttttct gtagacaaaa taaaaacaac
     2041 tgaaagaata aaatcaatga agctaaagaa aaaaatatta aaaagatatt taggctctct
     2101 ttctataaga aaaaggaaga ttacacttgt cgttccccat ttgatgaggt tcatcatgaa
     2161 gaaatcttta tacgtttcaa agagcgaata accacttgaa cgaaagatag cgattttccg
     2221 acaattgtag acaaaatata tgcataacat gacatagctc aacaagaaga aagaaaagaa
     2281 agtgagtaaa aatatctgta tcaagtaatg aaatgatctg cttaacactt tgacagctat
     2341 ctcttcagtc tgagtatacg gaaatgataa ctgcaagtta tcacgtaact gactttcttc
     2401 aatcagctca aataaccttt ctttcgacaa agagcctatc ttgatcttta acggtggata
     2461 gatttctccg ctgagaatat ttctttccca atacgtactg tttttcaatg tcagtatttc
     2521 aacaaccgga taatgttcga tccaggggat agaaggaata aatgtataaa tgggttgatt
     2581 gttttttata aatagaaatt tcggttcttt ttcactgatt tcttgataat attctgttat
     2641 ttgcgccagg tcatttttta aatctgtatg tctatatcgt tcagggatca atataacagg
     2701 gtgcgtttct ttttcggtta tttggatcaa ccttccagag acatcaatca aaggaaattt
     2761 cttaagataa ttcagattta tttttatccc tctgccaaac tgttcatttt cttcattcaa
     2821 gtaatctgat acattgacta atatacttcc gtttttattc aaagtttcat ataattcctg
     2881 atcggctgct gatgcaaaac gattggaata aataaaatcc acgtggtttt tcccgatggt
     2941 tactggataa aatacatgat agtcctcacc agcgatttct tttggtgttt catgggaaac
     3001 agctaactgt gaagaagaaa aaacgatagt tgctaattcg acattggcaa cggtcaaaag
     3061 aagagaaaac aacaatccaa atgtaaaaag aatgtattgg atatagttga acgcttctct
     3121 taacgaagcg attttttcaa ctaataaaag agaaatcaaa gagataccct atattccgct
     3181 gaacaagaaa acgtacatct ggaacaaata aagaaattgc tccttataat acataaaaat
     3241 aaaaccacca atgattcctg caattatgaa tgtaataatg attttctttt tccccaataa
     3301 acaccacaat acttgatgta atgaaaagcc attattcaac aataaactga tttctttatt
     3361 catcaataag aaaaaggatg ataacaaaag gaataaaaga atcaatccaa tagttaaata
     3421 cactcgataa ttatatacgt ctgtacttga aataaccgaa tacgaatagc tattttcaaa
     3481 gtcttcaaaa atattagtgg cataatcagt gtgaaatgct tgattgaatc gttccacaaa
     3541 tagtaaaatg aaccgttcat aggcttcttt ttctctcgtc atcaagaaaa attcaccttc
     3601 aaaatctttt attttgatcg attcttcaat ggacgttacg cgaaaggagg tattagaata
     3661 gggtaccttc tcgtgagaag atttgacatc cccttgataa tacaatgtca tttcaagctt
     3721 tggctgtatc ttgaagttcc aagatgtatc tacttcattg gattcattta ctgttttttt
     3781 catataagaa agctcagttt ctgatgagac attttcaaat accatgatta gctgctcata
     3841 ttcttcttgc ccaataaaat gttgtggcaa tcgaatcgga gtataagtat ctgtttgatg
     3901 gaaaaattca tttgtccgat agagtacctg tttatttata ataaccaagg caaaaccact
     3961 catgataccg gtcactatga ttgtgagtaa aactgtgaac ttttttttga ttatcaccac
     4021 tctcccttac ttaaatgatt ctttgataaa atcataccgt ttttcatcaa aaaaacgtat
     4081 ctatcaaatg tataggtcat gtagaataaa tctatatact gtagaatcga aaggagaaga
     4141 agatgaacat cggagaaaaa atacgaactt accgcagaaa ttgtaagctt acgcaaaaag
     4201 aattaggcga aaaaatcggt gtttccgata aaacaatctc tagttgggaa aattctcgta
     4261 caatgccaga ccttgaaatg ctgtcacttt tacatcagac tttaggtctg ccgattgatt
     4321 ttccttctat tgaatcagct accgaatcaa tagaaccaac attgccggat gaacctcttg
     4381 ttatcaagaa atcttattca tcaaaacaac ccttctcact caaaaagaca ctatctttct
     4441 ttctgctcgt cttttgtttg gtctatcttt ttcttatcaa ttatcaatat aaaaatgaaa
     4501 cggttgatcg attcaatcct tttttgaaac cacaaatcgg ctataccaca ctccctgtag
     4561 gatacgtgga aaattccatc gaacattggg tatgtaatga tatctttgtt catggtacat
     4621 ggctgtcttt tcacgctgga gaggtagaaa aaggtaagaa tcatgctgta atcaaacaca
     4681 aaggaaaaca cgtcatgaac gttcgcctgc tcacttggga agccattccc tcagattacc
     4741 aagaaatcct catccaagac tatgacgatg ccttaaaaag aagccctcag gagccagata
     4801 ggcattaagt aggagatgaa cgtagagata gaaaaaatca atgcacatga tcttatcaaa
     4861 aaaaaacagg ggtacaaatt aattgaaaaa gctttgagat gaaagttcct aaataaaaaa
     4921 gttcagaatg ataggtaaaa aaatgactaa ctattctagt attaggctat ttgtattatt
     4981 cttctgttat ttttatatca aaaaatccag aaataactaa atataggtat ttctggctta
     5041 tagtataata tttacgccac agcccccaat gattcttttt ggaatcatga tcaaggacgg
     5101 acatacacaa aaatattttg cccattcgat ccgaatagat taacgactaa tctagtaagt
     5161 aatatagatt ggtacgtagt caaaccagca aatggaaaag ttgtctattc caccccaagg
     5221 gctttaaaga cagcatcttc cggcgtttga taaaaaatca aattaaaggt acccattaat
     5281 tctgggggta cagttccaat atcagcagcg cttatcatag gtacaagtac ttttttcgct
     5341 ccggcatctt ttgctacttg taaacagtta gctaaattat ctaccttaat catagaacca
     5401 ccaattgtaa tatcccctaa aataactaaa cttggtaata caggtttatt taaggaaact
     5461 gagcataaag ctattatagt tgataacgat aatgtttttg tcataccaat gccattcata
     5521 tcttgaacat gaacgacaaa atcttttgtc gtcaaactaa tagactgact aacacttcca
     5581 taattagctc ttaaatatcg gtatgccgaa tccatcgctt ctcttgctcc agcatctgaa
     5641 ttgatcccag tacgttcaaa ctttccattt cccgaagtca tttgcgtttc caatttatag
     5701 gtacctaatt gcccagtttt gccataagat accgtgtaca aatgccctgg cttccctaac
     5761 ccttctggaa tcaaagttcc tccaccttgt tcaggaacag aaacatactc ttctcgctct
     5821 tcttctaaat caatgtatga aaaattgaca tcataaaatt ccattccacc aattttcttt
     5881 aattgttctt ttacacgacg acgcatttca agagcaaatc ttagtatttc ttcgacatca
     5941 cttttttcaa atctaccatc aggatacaac agtttcatca tcccagatac aatccgacga
     6001 actgcaatcg tatcccgctg atttaattga gaaccaagtt tgaaatattt atcaaaacta
     6061 tcgccaaaac taacttttcg aagctcacgc atgacttcag aaaaataatc tgtaatgaag
     6121 ccatagtcat ttgtaaaaaa atctggtcga tatttaggaa tttcccaacc tggtaaataa
     6181 caatgcatcc gatccaaaaa ggctgaatcc gtccccataa cctccggaaa tggctcaaat
     6241 aaattcgaag ttttcaatat tacatcaacg ctttgattaa tatttcctac aaaagccatt
     6301 gatgctgaag cattcttttc ttctttgcct ctagcaaaag atcctgaagc catgtaatct
     6361 ttcatgattt gaacgccatc attatcctta aatttaatcc cagcaacttc gtcaaaagca
     6421 acacagtccc acatacctac aagtcccatt gtacgactag ccatattata gaaaaggttt
     6481 gctactgttg tttgtccacc tgaaacaagg attgagtttg gtgaaatttc tttatataca
     6541 tgcgatttac ctgtaccacg tggtcctagt tcacagagat taaaattatt ttcaattaat
     6601 ggaataagtc ttgctaaatg aagccatttt acccttttat taaattgtgt aggttccata
     6661 ccaatggacc gtagaagtaa gtcaatccat tcatttgtag taaactcctt gcgagccatc
     6721 ttgtaagcct caatgtctaa accaggcatt tggattggat caaccttttt tgcaataaaa
     6781 gggctattcc aacgatcatt ctcatcatac caatattcca tcgaaacaat acaccagatc
     6841 cccccaccta ataatcgatc aaattttttc ggatactctt cactaatttg aacatttttt
     6901 aatcccaggt tagagaattc tgcttcataa caatctttct tttcatttaa atgtacagtt
     6961 actttatcaa taactgtata accacccaat tcacgaattt ttgcttttac tttttctgct
     7021 tcatcaggac gcacatagtt tcgagcaaga atatttttaa cattttccac acctttttca
     7081 accaatgttt catccaatga cgaagcatac ataccaagta aatattccaa tacataaaca
     7141 ggtacattcg caccttcttt aattttcttt gttaaatctt ttctgacaat ctttccggga
     7201 aagaattcag ataactttaa aatttcctgt tgttccacta gaattcctcc atgtgttcag
     7261 tcattataat agcaattaaa gtatatcata cttaaccagt aggcttgata atacaaaaga
     7321 aaaagtatcc acaaaaaatt gtggatacct cgtttttata tatcaaaatc aaatgattct
     7381 gcaaaaggat tatctataat gaattttatt cgctcggtca aatcattttt ttgatttttt
     7441 atcactaaat aataagtatc ttcaactgta tatcgacgat ttacaaattc aaaaacaaat
     7501 ttagtaaacc gctgatttgc atcttctgtt tttgaatccg caacatagat ttcttcattt
     7561 gagatacgtt caccatattc atcttcaaaa taaagccaga aagatgcggg gtcataaaca
     7621 tcactaattg catttttttg atagaattct aacgtggttg ataatcctgt aattctgcgg
     7681 ttgctagtca ttaattcaat tgctactggt tcctttgcat tgctaccttt ggctaccgta
     7741 atttctagaa cagggactag catttcttga atagatgacc ctccatgaac gtagctttgt
     7801 cccccgccag ctttcttaaa aatacttgaa gtaatcggga cacaaatgtt tctagtgtca
     7861 tcatttccta aagaatctcc caaagagtaa tttttcgtac caattatttc gtattcttct
     7921 tcactaagaa taaaacgacg ttctacacga tctttttcat ttctagatgg attttcaatc
     7981 ttatctactt cttcaacgtg gcttctttta taaataaatc catgatcagc cgtaactata
     8041 aaacgataga tactgccatc attatgcaag cgattaataa gtcgggaaat ttcgcttatc
     8101 gctaaatcac aggcctcaaa aactcgattt tcattcgagt gatcacctgt tctatccact
     8161 tgatcgtgat aaatataaat gactgatttg ccattaaata cttctcgtac ctcagcctta
     8221 ttcatcttaa ccacatcatc ataatttaaa gctacagcat ttgcatctcg ttgctggaga
     8281 atcgcatttc gattggataa accattcgtt tttttcccat caatcagtac ttctgttcct
     8341 tcattgtaag ttagttgatt gtttggtaat aaggcggctt taccgaattc agtaactgaa
     8401 ggtaatacag aaagaaagct agccatgttt gttgaatact tcttttctct aactaactta
     8461 cgttctaaat tcatccctaa ctcatatcga aaagcatctg aaataataac tacagtttta
     8521 acagagcgtt tcattacagc ttgattataa aattttcgaa tggatggacg attcttaaat
     8581 tcaaaagttt gattccaaat ttgtcctgat tgatcaagaa acttcgaata atttgcttca
     8641 actaatcgat acaattggcc atatttttgc ttttcttcta tatgatcact attccaaata
     8701 aatttccgat aatgcgtatc aacccaatac atttccttct catattgagt tagcaaatta
     8761 tccatgttca tcggcaagca caatctgttt ttatccacaa gataataagc atgtttcaat
     8821 aattgatact cataaatata atccgatgcg aaatgcattt tttcgcgctg ctttaaacta
     8881 tcaaaaattg tttgtcctgc aattttcgtt gtagtgtcag aagcctttaa tcgttctgca
     8941 taaaacatta atatttttgc atgaatctca gaaaatacgt ctgcttcaat tagatcatca
     9001 atttctatac tgtctaataa tccatcacca tcaatagcct gatatacttt tttagatagc
     9061 tgttcaaagc tttccatgta acgtgtatca ttcataaact gatccatgaa agtaataatg
     9121 tttgctttca aatgtagtat ttcaaatcct ttgaacttct tcggcatctc ttgatcaatt
     9181 tgataataaa aatagttcaa ataaagacac atcgtcagct tttccaatgt tggctggcta
     9241 tccgtaaagc caaatttcat ttgaataaat tcccaaaatt tagctgttaa ctgatactta
     9301 tcaagttccc ttaaaggatt ctccctactt tctgtaaagc tttttagaac tacacgcagt
     9361 aaatcattta atcgaatact tttcgatttc aacaaaacag ccattatcat taattcaatc
     9421 gtttctgatt tattaattgt catatgcgta ctaaattcac caaaacttct catgcgatcc
     9481 tttgcattaa agaaattacg atactgttta actgtctgat gaaatctaag tggaatagcc
     9541 aattcattca tgataatcgc taagcggtct gcactaaatt cctctgaata attcaataca
     9601 ctcaacaaat gattatcttc atctttatca tcccaacgag gaaatggcgc atagattaaa
     9661 tagttagttt cgggatcttc atattccaat aaacgcttag ttttaaattg tgcgtccttt
     9721 tctaatcgat agatttttgc attttgaatg accaatgagt caatttcttc aagaaaatcc
     9781 tgatttgaat caaaccaaaa aatgattttc ctacatccat aattaggaaa acgttgattt
     9841 aatttttctt caatttgttg cgtatttata tctgtcatca atctcaccta atcgttctct
     9901 ttctataccc cttatattga cataatttgg ataatttcgc acgcaattta agaataaaaa
     9961 agaaagttgt tagaaaacaa ctttcttaga taactatttt tttggttctt tgaccccttt
    10021 tgcaaaaatt tggaatttct cgccggtttt aggatcacgc tgcaacttat cataattgac
    10081 aattacacca tcatctaagt ctaattcaat tcgttttaat gctaagtgac ccaacttctc
    10141 gtcatactca cgaacttctt ttaattgctt ctgaatcttt acaatttctt ttgtcattgc
    10201 atttctttct tttgggttat cacttgttga aatttgttgc tgacgcaatt cgattcgatt
    10261 gtcatacgct ttttgtagtt cgtgtaggta ttcagtacgt acttttccta attgatctgg
    10321 tgtatagcga tgcaagtaca ttaacgcttt aaagccattt tgttttcctg agtccaattg
    10381 ccaataaatt ggtcgattgg agtatgtttt taaatggtct ttgtaaaaat cttgtacgaa
    10441 gtaacgacga ataatttctc gacttgtccc tttgccacct aaagtgtcag cgataaactg
    10501 gagattttct tcaagggttt ctttaccaaa agtaatctca acgaaatcaa tgaaacgatt
    10561 gacaatgtca atttggctat ccttgaaata ctcatcatcg gtgatgagta aaacattatc
    10621 agtatcaggt ttaaattttt gatagcggga aggatcccat tcgccgccag cgaagatgag
    10681 accatcttcg tctagggagt aacgaccgaa gatacaacct attgaataag aaataaaatc
    10741 cttaatttcc ctcgccttat caggtaacct tgcagagata tattcatctt gaacctcttt
    10801 tgttaattct ttttcaagag aataatgatt aatgaatatg tcgttcagtt cctcttcatt
    10861 cgattttaga gtattaaatt gattaaccaa ctgcctactt agacagttaa atgcctctga
    10921 gagatctttt ttaaacatat aacttaacaa aatgtttctt ctaaactcgt atgacgtttc
    10981 aagagcatta tagtcttttc tagctatatc gatattttca tccacttttt ctcttatttt
    11041 ttttaatttt ctctcagatg taactttaag aaaaggtagt tttcccattg gtccttcatg
    11101 gtaatccaag gtgggagaaa taaaagttaa aaagtgatta actaatttag agtttacata
    11161 gccaaatatt tcttcaaagt cagttatatc ctttgagaac agaacagatc ctttagtttc
    11221 aaaaattgcc tgattctgca atctaaaaga tacttgtcca gatgaaagtg tcgaccaagt
    11281 aattcccttt ttaaaatagt aattaagatt ttgtggtcta gattttaact tcccattttc
    11341 attaacataa ttttttattt catctccatc attctcccag ttaaccacgt attcattgtt
    11401 tccataccat tttctaaaac gaccaccttt agttgtagga aaccattttt ttctagactt
    11461 tttagcgtcc tctaatgttt tagcatcaaa ctttattttg ttaaattcta cttcaaacca
    11521 ataccgcaaa aatttattgt tatttccggt tgccaaccct tgttttgcat ctgcaacatc
    11581 gttagttaat tttccttgag caaaatcagt gattacgtta tcgcttgccc agtaagcaat
    11641 cggcattcct gggatttttt tgaagttgtc ttgattagta ctataaaaat aatcgcattc
    11701 accatgctga tgaattgctt ctaacgtttt aattttttga acttccattc cacctttaaa
    11761 atctgataat tttatatagc ttccatcttg ttcatttgaa ttttttatcg taaacgtaca
    11821 aattggaacg gttgcttctt caaaagctga atactccatt tgaatcaaac tagaaatgtt
    11881 tctttcatta attaaaaatg agcgtaactt ttcatagcta gaaatgaaca tccaaacaaa
    11941 tggcgtcata aaggaagcat atccattatc aatcagcatt tggctattca atttaataaa
    12001 cacagaaaat agatcttttt t
//
LOCUS       DAZKMB010000086        11733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000086 DAZKMB010000000
VERSION     DAZKMB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.086"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(366..1484)
                     /locus_tag="ACG8V3_002057"
     CDS             complement(366..1484)
                     /locus_tag="ACG8V3_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300577.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1717..1839
                     /locus_tag="ACG8V3_002058"
     CDS             1717..1839
                     /locus_tag="ACG8V3_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300578.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1955..3262
                     /locus_tag="ACG8V3_002059"
     CDS             1955..3262
                     /locus_tag="ACG8V3_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0300579.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3381..4580
                     /locus_tag="ACG8V3_002060"
     CDS             3381..4580
                     /locus_tag="ACG8V3_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0300580.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4607..5875)
                     /locus_tag="ACG8V3_002061"
     CDS             complement(4607..5875)
                     /locus_tag="ACG8V3_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0300581.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5930..5996)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6038..6684)
                     /locus_tag="ACG8V3_002062"
                     /pseudo
     CDS             complement(6038..6684)
                     /locus_tag="ACG8V3_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7092..7595)
                     /locus_tag="ACG8V3_002063"
     CDS             complement(7092..7595)
                     /locus_tag="ACG8V3_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0300582.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7718..8482)
                     /locus_tag="ACG8V3_002064"
     CDS             complement(7718..8482)
                     /locus_tag="ACG8V3_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0300583.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8590..8865
                     /locus_tag="ACG8V3_002065"
     CDS             8590..8865
                     /locus_tag="ACG8V3_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0300584.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9303..10253
                     /gene="yidC"
                     /locus_tag="ACG8V3_002066"
     CDS             9303..10253
                     /gene="yidC"
                     /locus_tag="ACG8V3_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0300585.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10432..10611)
                     /locus_tag="ACG8V3_002067"
                     /pseudo
     CDS             complement(10432..10611)
                     /locus_tag="ACG8V3_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11437..>11733
                     /locus_tag="ACG8V3_002068"
     CDS             11437..>11733
                     /locus_tag="ACG8V3_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300586.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         3560 a         2449 c         2076 g         3648 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt cagcacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaac atctttccat cggaaagatg
     3301 tttttttgct ttttctagtt taaataacag cttttttcat ctattttcgt tatactgtat
     3361 aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta gttctctatc
     3421 ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct tccttgttct
     3481 tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg ttggaaattt
     3541 tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt gctttttctt
     3601 atttcacaga aaaacgaagg ctactgaatg gtcttttatt caatgtttta ctcatcagtt
     3661 tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tatttttctt ggcggactga
     3721 tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat ggattgattg
     3781 tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca ttagctaatc
     3841 ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac tttttcttgt
     3901 taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg atcttgatct
     3961 atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag ttcaaccggc
     4021 ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg attgatcaat ggagaaacag
     4081 tttctcctct tctagccaag cgaatcaata aggcaattgc tttttatcgg gctcaaagcc
     4141 gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca gacgaaaaag
     4201 taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct gaaagagaca
     4261 ttcttgtcga aaccaattcg acaaccactt tggaaaacat gctctattca aaggaaatca
     4321 tggatcagca aatgaaaggg ccataccgcg cgatctttag ttccaacaat taccacattt
     4381 ttagagccgg tctttatgcg cggcaagcca aattgaaagc aaatggtatt ggtgcaaaaa
     4441 cagctttcta ctacttgcct aatgcgtttt taagagaata cctcgccatc ttgatgatga
     4501 acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca ttcttatcgc
     4561 tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac accttgtttt
     4621 tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc gctacttgct
     4681 gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag atcataaaga
     4741 agtatactgc cgcttgtgaa ccgaacaatt cgatccccat cacaaagcag gcaatcggtg
     4801 tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa ggaacagaaa
     4861 catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac aatggtgtca
     4921 cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac ttcccaataa
     4981 aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga ctcaatccga
     5041 gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca cctatcgtat
     5101 ttcgacaaac aggatgagga atccattgtg catatcgcgc ttttaaaaag acgatcgaac
     5161 ggctgaatac ccaaccaatc aaaccaaaag caatcccagc aacaaaaact ttgaagaaca
     5221 gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa acaccatagc
     5281 tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc gcttctgttc
     5341 ggactttccc gattgccaac acttctaatc caaataatgt tccagctaaa ggtgtgccaa
     5401 atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt tctgatttat
     5461 ccaatcggaa caaacgtgcc gtattatctg ctaacgctcc ccccatttgg actgctgttc
     5521 cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac agtgtaagtg
     5581 gaataagccg caacgggatt ttttcttctc caccatttcc ttgatcgatc actaagttgt
     5641 ttccacgact agaaagtccg ccgtgatacg catagagata agcaaaaagc gcaccactaa
     5701 ctggtagtag acaaaggagc caaggatgtg atagtcggat atctgtcaca actgtcaggc
     5761 tatttaagaa aaaagcagac aagctcccca tgaatatacc gagaatcgct gtgatcacca
     5821 gccacttcag catatacact aaaatgcgta ctggactcgg taattgtttt tccataaaaa
     5881 attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga ctttagtagg
     5941 cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat tcacttccat
     6001 taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc tttacacgtt
     6061 cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg tcactatcat
     6121 gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc gttgtagaaa
     6181 gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta tttatcgttg
     6241 gtgataaagt accgctaatc ccttttaatt tttcatcttc gtaatagacc atgaattttt
     6301 tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg atttcattcc
     6361 aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt ggtttgctag
     6421 gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct gcttgcgctt
     6481 ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gattctggca gttcgtgatc
     6541 taatatgtct cgaatctttt cagctgctag tgcctgaact gtttgtaccg tttttggatc
     6601 attgacagat gagtacgcat ttaataactg ttctacttct cttattataa aaaaatattg
     6661 gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca aagtgcatac
     6721 tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatccc ggactctttt
     6781 aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattat tctatgtaca
     6841 tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac tgcaaacaga
     6901 aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat ggtgttcaaa
     6961 agctggcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagca attttcgtaa
     7021 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg gtctattatt
     7081 ggttttatct attaaatcga ggattctttc ccaaagtaac gttgccatct cttacgcata
     7141 cttgctgaca taggctgagc ggcttctttg actgcgcagt atttgtctac gaatgttacg
     7201 atatatcctt ctttgtactt aggcggtgca atcgttgcgc cccacatgtg cttcaaaatg
     7261 atatctcgct caagatccga caattcggtg atcttttctg cattctttac agcaattctt
     7321 ggatggatat acgcatgtgt gccttcatca aatttcgtcg tacgccaatc atagtagaac
     7381 agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca tttttttgcg
     7441 agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga atgaacatgc
     7501 tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttc tacataagac
     7561 atatattctt cgtcttcacg ccatttttct gtcataatta tcgatctgcc acctttttga
     7621 atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt attctagtcg
     7681 ccttggataa aatagccaaa gcttaatagt tccttaatca taaagaaaaa tgataaagaa
     7741 caatccctgc cgcaacccca acattcaacg attccgcatt tcctttcatc gggatatata
     7801 cgttctgatc agtcaaagac aagatttcct gacttacccc ctgaccttcg ttccccatga
     7861 ttattgcgaa gttatctgat ttttctactt tgtctaatgc gtgtgcttct tcatttaatt
     7921 ctgtgccata cactgtaaac cctttgtctt tcattaaaga aatcacttct gtcaaatctt
     7981 tttgcaacac aggcaaatga taattgcttc cctgcatgct gcgcagcacc tttgtactat
     8041 aaatatctgc cgttcctttg cctaataaaa caccagccat atcaaatgca tctgccgtac
     8101 ggatcatcgt ccctacattt ccaggatctt gtacattatc taacaacagc catcctcctg
     8161 aaaaatctgg cgtgccttca gccgttaacg ccatcactgc taagattcct tgtggtgtag
     8221 gtagatccga tacagcatcc atgacctcat ccgaaactag aaaagtagaa atctctttgt
     8281 ttgcggccag ccagtctccc cattctttca tgcctcgctg tgtcacaaaa agttctttta
     8341 tttctgcatc agacgacgca gcttcttcaa tcagatggaa cccttccaaa agatattctt
     8401 ccttttcttc ccgatatttc tttttatgta gtttttttaa ttctttgatt cttggatttt
     8461 ttgctgattg gatctctttc acattcctca cctccccatg attatatcat ggaataggtg
     8521 cttgagcttg tttcatccta aaaaaatcgg tatcattaat ttataaaata ggaaagaaag
     8581 gtgacgaaca tgcgcaaagt taaaatgaac gtccaaggac gcgtccaagg cgtaggattt
     8641 cgctacatga ccaagatggt cgctgatcaa ctaggcgtga ctggaacggt aaaaaacgaa
     8701 gatgacggct cagtcacgat cgaagcaatc ggcaatgacg acatcatcca gaaatttatt
     8761 gaagaagtca agaaatctcc gagcccaagt ggacgagtgc agtatgtgga tgtccaagaa
     8821 gacccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg tgaaaaagct
     8881 ggtctgcatc aagcattaag gacaggttgt gaaaaatagc tctccatatt tttcaacagg
     8941 ctggacttaa tgccgtagat gcaagctttt gaacaccgtc gataaggtta ttcttgaggc
     9001 gctttgcctc aaaaattaag gacagcagtg aaaaagctga tctatcactt gcagtgagtt
     9061 catttgactc cgctttttcg ggcagaaaaa aagtttttag tttattaaag taagaaagaa
     9121 acacttggaa tcattctact ttttcctagt atttctttct tgattttttg tctttttttc
     9181 aaaatttttc gctacgtaaa agaacgaata tttatatgaa atttctatga agttgattga
     9241 attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag aggaagtatt
     9301 ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt
     9361 tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcggga atcgtttatc
     9421 gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgaaaat ttcaattggt
     9481 cttatggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc ttgccactag
     9541 gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac
     9601 aagttgaagc agcccaacaa aaattgaaag ctgcaacaac acgtgaagaa caaatggcag
     9661 cacaagcaga aatgcagcaa gtataccgcg aaaacggatt gagtatgacc ggcggaatcg
     9721 gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtacttt acggcacgtt
     9781 atacagaagg tatccgggaa tcatctttct acggcatcga tttaggctca ccaagtatgg
     9841 tacttgttgc cattgcaggg gtcgcttatc tgcttcaagg atacatctca acgatcggta
     9901 ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct gctatgatcg
     9961 tcttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt
    10021 tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag
    10081 tggcagaaga gttgaaacaa aacccaccaa aacaagtcgt cactccaaga attgatgtga
    10141 cagaatcagc taaaccagct gacagcaaac aagcaaccaa acaaatcaag caacccaaaa
    10201 acagtacaaa caataaaggc cgcaacgcag gaaagcagca gaagcggaag tagatagtaa
    10261 ataagagtgt aataaaaatt cattaacagt tttagcaatt aaaaatatcg accaagattt
    10321 ttgaaaaaga aatctggtcg atatttttat ttaagtgaat gtttaatttg aatcatcgat
    10381 tgatcgctat ctaattgcag cagcatcttt ttatgaagaa acaacttttt cttatctatc
    10441 aacgtagaca ggtgttgctg tttcttctaa tagtgtactt aattccttaa tattttgttt
    10501 tccttcttct aacatccaac tactcgcttt ttcttcgcct tcggtaacaa aaggcgccac
    10561 tcccttcgcc catgttgctc gcccacataa gacaccattc tccataaagg acaacgcgga
    10621 gataccaccc atctaaataa catccataaa cctttctagt agaagaatag tgatagtatc
    10681 catccctttt tctactggga atacaattac acgattcaaa gcttgataca caacatttcc
    10741 cttcttataa accgctattg taaccgaatt gttttgaatc acctttttgg gatagaaact
    10801 ggctcctttc cgcaacttct taaatgttgg tttcttttat actttctttg tagcaactac
    10861 aaatttcttc aaatctctaa atgtttgcat aacaaccgcc tttctgactt ttactcagga
    10921 tggtatttaa aaatcagcaa aataaggctt ctctccatct tttttatttc ctagctgctt
    10981 taaagacact cttgctgttt tttaatctct atcaaggggg ctctgaatct attttgatga
    11041 acttatctta ttaatttctc aaatgagctg tcagacacaa ctgtattttt tcaatctcat
    11101 accggacaac tatgccagct taaaaaaatt tcctctggca aaacttcttt attgaaaaat
    11161 gacaatgaaa aaagaccatt ctttttgttg aatgctcatt gtgtaaacat atttcctgcg
    11221 actgctcttc gaaccattct cagtgcaatc cctaatttat ctattaaatc ttcttttagt
    11281 tcctcatctt tcatattcaa aatttccttt gtatgttaca caagtttgtt ctctaaataa
    11341 cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac tcaaattgta
    11401 tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca tcttaccata
    11461 gacgaactga caatgataga atcatattat cttcaacata ataagccggt tgaaatcgct
    11521 aaccgaatgg gacgtgctat acaaactatt tataatgtag tcaataagtt caagcaaggt
    11581 aagactgctc ttgattattg gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa
    11641 gtcattcaat tacctgctca tgaagtagat tacattaaag agaaagtcac tcttggttgg
    11701 acgcctgacg tcattatcgg gcgaaaagaa agg
//
LOCUS       DAZKMB010000087        11602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000087 DAZKMB010000000
VERSION     DAZKMB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.087"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8V3_002069"
     CDS             6..305
                     /locus_tag="ACG8V3_002069"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0300587.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8V3_002070"
     CDS             378..1739
                     /locus_tag="ACG8V3_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0300588.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8V3_002071"
     CDS             1761..2021
                     /locus_tag="ACG8V3_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300589.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8V3_002072"
     CDS             2035..2787
                     /locus_tag="ACG8V3_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0300590.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8V3_002073"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8V3_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0300591.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8V3_002074"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8V3_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0300592.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8V3_002075"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8V3_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0300593.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8V3_002076"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8V3_002076"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0300594.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8V3_002077"
     CDS             7161..8558
                     /locus_tag="ACG8V3_002077"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0300595.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8V3_002078"
     CDS             8639..9079
                     /locus_tag="ACG8V3_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0300596.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8V3_002079"
     CDS             9227..9553
                     /locus_tag="ACG8V3_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0300597.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8V3_002080"
     CDS             9554..10303
                     /locus_tag="ACG8V3_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0300598.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8V3_002081"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8V3_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0300599.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8V3_002082"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8V3_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300600.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3661 a         2014 c         2357 g         3570 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa ac
//
LOCUS       DAZKMB010000088        11496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000088 DAZKMB010000000
VERSION     DAZKMB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.088"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..252
                     /locus_tag="ACG8V3_002083"
     CDS             85..252
                     /locus_tag="ACG8V3_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0300601.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(312..665)
                     /locus_tag="ACG8V3_002084"
     CDS             complement(312..665)
                     /locus_tag="ACG8V3_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300602.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1169..1588
                     /locus_tag="ACG8V3_002085"
     CDS             1169..1588
                     /locus_tag="ACG8V3_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0300603.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            1614..1820
                     /locus_tag="ACG8V3_002086"
     CDS             1614..1820
                     /locus_tag="ACG8V3_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300604.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(2048..2221)
                     /locus_tag="ACG8V3_002087"
     CDS             complement(2048..2221)
                     /locus_tag="ACG8V3_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300605.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            2233..2862
                     /locus_tag="ACG8V3_002088"
     CDS             2233..2862
                     /locus_tag="ACG8V3_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300606.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(2944..4110)
                     /gene="dnaJ"
                     /locus_tag="ACG8V3_002089"
     CDS             complement(2944..4110)
                     /gene="dnaJ"
                     /locus_tag="ACG8V3_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0300607.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(4261..6090)
                     /gene="dnaK"
                     /locus_tag="ACG8V3_002090"
     CDS             complement(4261..6090)
                     /gene="dnaK"
                     /locus_tag="ACG8V3_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0300608.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(6141..6704)
                     /gene="grpE"
                     /locus_tag="ACG8V3_002091"
     CDS             complement(6141..6704)
                     /gene="grpE"
                     /locus_tag="ACG8V3_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0300609.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(6798..7841)
                     /gene="hrcA"
                     /locus_tag="ACG8V3_002092"
     CDS             complement(6798..7841)
                     /gene="hrcA"
                     /locus_tag="ACG8V3_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0300610.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(8075..9240)
                     /gene="hemW"
                     /locus_tag="ACG8V3_002093"
                     /pseudo
     CDS             complement(8075..9240)
                     /gene="hemW"
                     /locus_tag="ACG8V3_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(9256..9483)
                     /locus_tag="ACG8V3_002094"
     CDS             complement(9256..9483)
                     /locus_tag="ACG8V3_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0300611.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            9967..10574
                     /locus_tag="ACG8V3_002095"
                     /pseudo
     CDS             9967..10574
                     /locus_tag="ACG8V3_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            10747..11460
                     /locus_tag="ACG8V3_002096"
     CDS             10747..11460
                     /locus_tag="ACG8V3_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300612.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         3340 a         2318 c         2009 g         3829 t
ORIGIN      
        1 gttaaataag atgaaatatt ttttaataaa gatttgaatt aaagtgtaaa ggaggagata
       61 gttattataa actacaagtg gatattgtgt cctgtatgtg gaaataaaac acgattaaag
      121 ataagggaag atactgaatt aaaaaaattc cccctctatt gtccgaaatg cagacaagaa
      181 aatttaattg aaataaagca gttcaaagta actgtgatta cagagccaga cgcaaagacg
      241 cagagccgat aaaatgagat taatacaatc tcattttatc ggctctttcc gttatgtatg
      301 gattctttta attagtcttc gatgtttctt gcttcgttga taccgctggc taaagattcc
      361 attaaggata gttctttgtc tgtaaagcta tccatgtatt tctctatctg taatcgtcgg
      421 gtgcttttta ccaagttatt agcaggtaag aaaaattcat caacggaaac atgaagtaac
      481 gatacaaggt cataaagaac ttgtatgctg gggtgttgcc ctttattttc aatattagtt
      541 aagtaccgtg ggtcaatttc aatcaatgct cccacttgtt cacgagttaa acctcgtttc
      601 aatcgagctt ctttaatggc taaaccaaag gctctaaaat catatttatc ttctttttta
      661 cgcatagtag accacctcta tacattttat tgttcctact gaattaaaaa caggtataga
      721 aaaacgtgtt atatggttta taggtttata tttaataaaa agcactacta aacgccaata
      781 aaaaaaaccg ttatatggta gtgctattta cgctgttaaa atattgtata ttacttccaa
      841 atggcggttt gttggaggtc aacgtcgcca tgaagtacat catatacaat aaatttcctt
      901 acattgggtt cttgtcaaaa aaagtcgtct atctgcaata gataagtacg tccaccaatg
      961 tggttttata aatcatatag atagaataac agaagcatgt aaacagagaa ataaatctgt
     1021 ttatatgctt ttttggctat tcagaacttt tttacaaagt ttatttatca gtaatgcaac
     1081 aaatccccct ttcacattgg gactaagagt gaaaggagat aaacgagcaa ggctcacttc
     1141 ctttcctaga cagaaagggg gtgagattat gaggacatct tctttcgagc atatcgtcag
     1201 attacagttc aattctttaa tgctgattgt cattaagaac aaagtgaaaa gtcgaaacag
     1261 acagcttgca agacattcta aacgtgaagt attgttttgt gaaatagccg aaacgaaaca
     1321 agttgaacgt ggaacgattg atacctgttt ttatgattgt gtatcattta aagtcttgca
     1381 ctttacaatt tatgtatctg atgaaacact tggtacagtt ttacatagac tatcagaaaa
     1441 acagcgtagt gcgattttat tacgttattt tcaaggaatg aacgacagag aaatttcaga
     1501 actgtaccat gtatcacgtt ctgctatttg cagtagaaga actagaggac taaggaagtt
     1561 aaaaatgctt ttgagcgaaa ggaagtaaag attatggcta tccctctttt gaattgattt
     1621 gcaaagcgtc aagcggagat gaaatggcag ttaaagagat tttgaagttt tatgacggtt
     1681 atatttctaa attatgcttg cgtccttttt accactctga aagtggtaaa atcattatgc
     1741 aggttgatga agaactaaaa ggagaaattc atacagacat gatgaaagca atattgaaat
     1801 ttgaaatcgg agtgaagtaa tcaactgtat aaaaaaatcg ggagatacac agtttcgtgt
     1861 tatctccctt agtagtttta cggtcatatc aaggctcatt caatcgtggt aaattcgtgg
     1921 taagatgagt gtttttctat acttcaaaat gcttgaatgt atagtgttta tgcggataat
     1981 tgaaaattag atataaaatt taataaaaac caaaaatgca cggtcgccat gaagggaccg
     2041 tgtattttta tcttcttatt ttctcaccta atcgtatcag ccgcatcgct tgttttcctg
     2101 cacaaacaag caatcgctct acctgttcca tcgcataaga aagtaccatc ggttcattga
     2161 tgatatcaag tacatagtcg ataccaccct cacttaattg agcttgattt ttcttctcca
     2221 tattatgtag ttgtgggaca actttctgac aatcagcaat tgtgtcaaga acggatcaat
     2281 cctttattgg agttgctcga aagggtcttc tcttattacg gacaggcttt gctcactgta
     2341 catagacaac aaatcattat cctagtcaat cggatttcca gagcctcatt gctatcattg
     2401 cttgataaaa tccagacaaa gtttaataaa atgtatcagt tacagctaaa ctttggaatt
     2461 gggtcgcttt gttatacaga gcaagagaca ccgcaatctt tccttcatgc caaacaagtt
     2521 tgcgaatgga tagctttcca tcaatctgtg aacgagatcc gcttttttga agatttagac
     2581 ctcgggatcg ttttaccagc aatcccgtca gatcaacgaa cattatatgt aaaacgtatt
     2641 ttgaaaagcc tgacagaaga agaagtacat ttgttcaaga agactttggc atgcttcagt
     2701 aaaaataacg gaagcatcaa aaattgctca gaagaattat ttattcataa aaacaccttg
     2761 caatatcgat tgaacaagtt ccattctctc actggttata cccctagaaa ctatgacgat
     2821 tatcatattc taaagctggc ttttttgttg gtacaaacat gacaactaca aaaaaaccag
     2881 gcgaacatat gattgttcgt ctggttttta attattaatg acttagcgtt tttactagaa
     2941 aaactacttc cgctttttcc cgccaaaagc atctttaaac ttatcgaaga aaccgtcttc
     3001 ttgctgttct tcaactttga tgccgccagc ttcagcgaat gcacgtaatg cttctctttg
     3061 ttgttcattt agatttttcg gtgtgatcag ttttactttg acatgttggt cgcctgttgc
     3121 accaccacgt aacttcggag cgccttttcc acgtaaacgg aagttcgtac ctgtctgtgt
     3181 tccagcaggg atcttcaatt tcacatctcc atggacagtt ggtacttgga cttcatcccc
     3241 caaagctgct tgtacaaaac ttaaaggcaa ttcatagtaa atttcagaac cttcacgatc
     3301 aaagatatca ctttcttcta cacggaagac cacatacaaa tcaccaaatg gtccgccgtt
     3361 ttctccagct tctccttgac cagctaaacg catttgttgt ccttcttcta caccagctgg
     3421 tacattcact ttaactgaat gtgctttctt ctcatgtcct gagccatgac aggttggaca
     3481 aggttctttg atttctttcc ctgtcccatg acaaacatca caagtttgac ggctcatcac
     3541 tcgtccaagt ggtgtttggc gttctacatt aatcgtacct gcaccatgac atttatgaca
     3601 agtagttggt tgtgtgcctg gttttgcacc gttcccacca caagtatgac agatttcttc
     3661 ccgattatac ttgatttctt tttctacacc aaaaatcgct tcttcaaaag acaagtcgac
     3721 cgtatattgc aaatcagcac cttgacgagg cgcatttgga tcgactgacc gaccgcctcc
     3781 tccaaagaaa gagtcgaaga tatcttcaaa accaccgaag ccgccaccag agaagccgcc
     3841 gaatccacca aatccgccac cagctccacc agcaccgtaa tttggatctg tgcctgcatg
     3901 cccgtattgg tcatatgcag cgcgtttttg cgggtcactt aaaatctcat atgcttctga
     3961 tacttctttg aatttttctt ccgcatcagg ttctttattg atgtcaggat ggtatttttt
     4021 cgaaagctta cgataagctt tctttatttc atcttctgat gctccttttg agagccctaa
     4081 gacttcataa taatcacgtt ttgttgccat aggctttcct cctattggtt ctcatcattt
     4141 tcacgtcgct tgttatcata tcatattttg tttgaaacaa aaaccgatac ttcaaactat
     4201 ttttcaaaag aaaaagccaa agtagcaact ttggccttct ctcgaaattc agtgaactga
     4261 ttatttatcg tcgccatcga cttcttcaaa atcagcatca acgacatcat ctgctccgcc
     4321 ttgagctgct tctgggtttt cttgagcttg ttgttgtgcc gcttgttcgt atagtttaac
     4381 agtcaagttt tgaacgattt cgttcaatgc atcgcgttta gttttcattt cttcgatgtt
     4441 gtttgcttca acagcggctt tcaattcatc gcgagctgtt tctgcttttt tcacttcttc
     4501 ttcgtccact ttgccttcta attctttcaa tgttttgtcc acagagaata gcaatgcatc
     4561 tacatcatta cgtaggtcaa cttcctcttt acgagcttta tctgcttctg catttgcttc
     4621 tgcatctttt accatacgtt cgatttcttc gtctgttaga cctgaagaag atttgatcgt
     4681 gattttttgt tctttttgtg tacctagatc tttcgcagat acatttacaa tcccgttttt
     4741 atcaatatcg aatgttactt cgatttgtgg aataccacga ggagctgctg gaatatctgt
     4801 caattggaat cttcctagag ttttgttgtc agcagccatt gggcgttcac cttgtaatac
     4861 atggatgtct actgctggtt gattatctgc cgcagttgag aatacttgtg atttacttgt
     4921 tggaatcgtt gtattgcggt caatcaattt agtgaacact ccacccattg tttcgatacc
     4981 taatgacaat ggtgtaacgt caagtaaaac aacatctttc acatcaccag tgatcacgcc
     5041 accttggata gcagcaccca tagcaactac ttcatcaggg ttaactgatt tgtttggttc
     5101 ttttcctgtt tctttacgta ctgcttctac aactgcaggg atacgagtag atccaccgac
     5161 taaaataact tcgtcgattt ctgattgtga taaacctgca tcttttagcg cttgacgtac
     5221 tggtactttt gtacgttcta ctaagtcagc tgttaattca tcaaattttg cacgtgtcaa
     5281 tgtcatttcc aaatgcaatg gtccagattc tcctgctgtg ataaatggca agctgatttg
     5341 tgtgcttgat acgccagata aatctttttt cgctttttca gcagcgtctt tcaagcgttg
     5401 taaagccatt ttgtcttgag acaagtcaat gctgttttct tttttgaatt ctgctaccat
     5461 gtagtcgatg attttgttat caaagtcgtc cccacctaga tggttgtcgc cagctgttga
     5521 caatacatca aagacaccgt ctcctagttc aaggatagat acgtcaaaag taccgccacc
     5581 aaggtcgaat actaagattt tttcgtcgcg atctgtttta tctaaaccat aggcaagagc
     5641 agctgcagtt ggttcattga cgatacgttc tacttctaga ccagcgatct taccagcatc
     5701 ttttgttgct tgacgttgtg catcattaaa gtaagctggc acagtgataa ctgctttttc
     5761 gactttttca cctaaatagt cttctgcgaa tcctttgata tattgaagga tcattgctga
     5821 gatttcttgt ggtgtatatg atttaccttc tacatctact ttgtaacctg cttcaccgat
     5881 atggcgtttg attgatgaaa tcgtatttgg gtttgtcaca gcttgacgtt ttgcaacttc
     5941 ccctacttgg atttctccat ttttgaatga tacaacagaa ggtgttgtac ggtttccttc
     6001 tgggttagcg atgatttttg cttcgtttcc ttccaatact gctactgcag agttagtcgt
     6061 acctaagtca ataccgataa ttttactcat aattaacgtc tccttcttta attgaaagtg
     6121 taagttttat atccgcacaa ttattgtgca acaatgacca tggttggtct taacacgcga
     6181 tcatgtaatt tgtagccttc ttgtagtact tctacaatcg tatcggctgg tgtatcttca
     6241 gtcgctggaa cagtttgtac tgcttgatga agattaggat caaaggcttc tcctttagca
     6301 gggatttttt caattccttc ttcttccagt gcgttacgca aactttccaa gaccatttcc
     6361 acgccttttt tcaaacctgc cccttgatca tctgacactt ctgtggctaa tgcacgttcc
     6421 aggttgtcaa ttgatggaag taattttttg gctaaatctt gtgaacggta tttttgtaat
     6481 tgctcacgct cattttttcc tcgatttgcc atatttgcga tttcagcacg agcccgcaag
     6541 tatttatctt ccatttcttc taactcagct tttaatttcg ctgtttcgaa ttcttccgct
     6601 tcagcttctg aaatacccga atcttctgca gcttcgacat cgatttccgg ttctggctga
     6661 gcatctgtca tttctttatc tagttcttct tgattttctt tcacgataca gtcttccttt
     6721 cattattcag tcagctgttt gattgctctt actaagaaag agagaacgat cagctgcaaa
     6781 tagcttcgtt attcacatta cgagtttgac aagtcaagcg aacggtagta atcagcaagt
     6841 ttagctgcta gttctcttcg ataaacatcg actagaccga acatccgaga ataaggcata
     6901 ctagtaggcc ctaacaaggc tattgtacct cttccatgac catctatctc gtaacttgct
     6961 tgaatcatac tcatattctg caataaatca ttgcctaatt cagcaccaat cttgatatga
     7021 atattgctat ccatcggagt gatcatttgt gtaagtccgt ctgagtcttt cataaaagaa
     7081 aacatcgatt tgaactgatc aagatcttga cggggctcat aatccaagag attcatttgt
     7141 ccactgacat aaaccttatc ttcaaaaaca tgaccaagaa cggtgtcaaa taactgagac
     7201 atcccttctg tcgtttggaa atatttatga agaatcatcg gaatctccgt acgaagttta
     7261 tggtacaccg tcatcagagg atctccgact agacggtcgt ttacaatctg taccattttt
     7321 tctagttcct gacttcccat atattcggga agagtgaaca cttggctttc tacattcccc
     7381 atatctgtta cgacgatagc aataatctgt cgattattca agggtacgat acgaaagcca
     7441 gtcagtcggc ggtcttttat ttcaggtccc aatgaaaaag tcgtatagct tgtcagctca
     7501 gataatatct cagcagattg cttaataatc tcattgattt catgaaattc tctgcctaga
     7561 gactgacgaa tcagcagcaa atcatcttct gctacgcgag caggttttaa caaatgatcc
     7621 acgtaataac gatagcctaa agcagatgga atccgcccag aagaagagtg agtcttcaac
     7681 agcaatcctt catcttctaa tgctttcata tcattgcgaa tcgttgctga gctggccttg
     7741 actccctcag acatcaatgt tttggaacca acgggcacgc cactactcgt atagtgttgg
     7801 atgatcagac gtaaaatgtc gttttgtcgc tttgttaaca tcttcctcac ctctcattag
     7861 cactcatata ttctaagtgc taatccactt ataaatatag caagaagccc ttctcctgtc
     7921 aagagaaaga ctccattttt tagcactcta aacacaagag tgctaattct aaataattga
     7981 tgtaaatcca gttatatcaa cgtttcaata gtcctttatt gtatttttga acttttttta
     8041 tttaaattca gataaaaaac aaatattctt taaactattt ttcaaacaaa aagcgctcaa
     8101 acacattgtt ccccaaaaag gtccctcttg gtgtcaaata aacacggtct tcttcattaa
     8161 caagtaaccc ttcttgctct aattccctta gcacatcacc gtacaccgat tcaattgttt
     8221 tttgatagcg atcatagaaa tgttttttag taatccctgt ttttttctta gtcctaagaa
     8281 catttcttct tctatttgat tttttcgtgt caatttttct tcttccaata ccgggcgttc
     8341 atttgctcgt aatgggcgta aatagtgttg gattggtcct ttgttgcggt aacgaatatc
     8401 tccaagataa ccgctagcac cagctccaaa cccaaaatag tggtcattgt tccagtacat
     8461 gaggttatgt tgagattcat gtccggagag cccaaagtta cttatttcat attggtgacg
     8521 tccatgcttt tccattgctt caatcgtctc ttcaaacatt ctggtttccg tttcctgatc
     8581 aggcagttcg agtcttcctt gtcgtaccca gttcatgaac attgttttgt tttcgaggat
     8641 caatgagtac aaagaatagt gaggcagatc caaagccaaa gctttttcca aagtatccag
     8701 atatccttct agtgtttgtc ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa
     8761 attttctgct tctaaaaatt gcatagtttg atacacatct tctgcggtgt gtttacgtcc
     8821 aatttttttc aataagcgat tatcaaatgt ctgcactccc atggataaac gattgacgcc
     8881 atagtttttc attacttgaa gtttttcacg ggtcaaatca cctggattcg cttctacagt
     8941 aaactcatta ccattttgaa aaggcaggat ctctctagca cctgataaca gtcgatcaag
     9001 ttgtttagca gataaagaag ttggtgtccc gccccctacg taaagtgttt ctaaattcgc
     9061 tgtcggatga ctttctagca ttattttcat ttcattcagc agcatttcca catactcatc
     9121 tactggctgc ccttctaaaa aaactttatt aaaatcacag taatagcaga tatgctcgca
     9181 aaatgggata tgaatgtacg cagaaacctt gttttctgcg ggatttattg tttctatcat
     9241 tgaaattctt tctccttacg tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg
     9301 ttcaagcacc aactcattga aataagccgc atatttagaa tcaaggaaac ctagctctaa
     9361 aaatgcttgc ttattgtcat cggagctaca cggtgctttt acagcctctc gataaacggt
     9421 aatcgcaaag ctgaccttcg gcattaagtc aaaatctgca aaaatatgaa aagctatttt
     9481 cacagattcc tccttaagtg ctcagaccac aacgctttgc ttacaacctc tcatatacgc
     9541 atactaaacg tatttggcga acaaatccac taaaaaatcc aagaatcgac ttcttggatg
     9601 attttttctt tcattaaact ggttacctag ttatatatct ctagggttgt tttcatgtct
     9661 gagtgcccga tatgatttat ttttcgtttc tataattact ttttgccttc cagtttcttt
     9721 taatattgta ctcaagcaag tgggaattgg ttcttttgtc atccatgtat gcaaaatgtt
     9781 ataatgctgg taaacaaaaa tgattacctg tcatttttat ttagttcgta tttaattaag
     9841 ttgactatct gtatactctg tctaaaactt aagaattgtt gttttcgctg tcgctaccca
     9901 attttaatga atgtctgtag gttaaaactg ttgtccttgt tatagatagt aaaggagagc
     9961 ttgaaaatga aaactgcgat tacgtttcta ttagaggaat atgccgattg ggaaggtgct
    10021 tatattacta gcacactaaa ccaaaatgag aaatggcaaa acaaaactgc ttctatccaa
    10081 tcagaggtca cttccattgg aggattaaaa acaaaaatcg attacttaat cgaacaaata
    10141 cctgaaaaag ttgacctttt tattatgatc ggtggcaatt catgggacat aaaaaatcat
    10201 catttatatc aaataatcga taaattgtta aaaacagacg taccagtagc tgctatttgt
    10261 ggggcagttg attatttagc tcagagcgga ttattgcaag gttacaaaca caccggaaat
    10321 gcgcaatttc cttggttaga atcggaaaat tatcagaata aagaagattt tatagaacaa
    10381 caagctgtga aagatcgtaa tttgattact gcgaatggaa cagctcctat agaattcaca
    10441 gacttgattc ttcaagcagt ccattttgaa acagaagaaa taagagaaaa aacgattcgt
    10501 ctatacaaac ttggattcta tgattactat aaaaagtacg gagatccttt ctcctaatca
    10561 ttctcttcaa ttaaatactc cttatcaata gtgtgacaag taattttact gatgattatt
    10621 tgtcacactt tttctgacat ttatcaaaga agatacactc tatttatatt catagacaag
    10681 gttttacgga ttcaaaaatt caggattatg ctatactggt attaaaaaaa taatgaggta
    10741 ttttatatga aaaagttagg ttttatttta ttgttggtct ctttattcct tgtaggatgt
    10801 acgtctccta gtaaagaaaa tagtgatcca tctgcgacca cagacaaaag ttcaatcaga
    10861 caaaccagtt cagctgaaaa atcaagtact cagaaatcag cttctactga aacgaataca
    10921 tcttcaacaa gcagtaaagt ctcagagccc aatacttctt ctacatctag tagtacagtt
    10981 gagacaaata tttcttcttc aacaatagac acaacctcct cacagtcttc tcagtcaata
    11041 gatccgttag caggatattc tgatctacaa attgagtacg cacgtgtttg gctagctatt
    11101 agaggtaata cttttagaga tacatcgggt gatttcgaat tacatgttat tcattatcct
    11161 gcaggaacac ctatcgcccc ttatgatgca gggagtgcag tctatccgaa agatgtggtt
    11221 atgctcactg ggaaatacag ttaccaggga gttttagtgt attcaagtaa ccatgacggg
    11281 acaatcacta gttatccagt tccttcacat tggcaaatgc ctgcagatca aacatcttat
    11341 ccagcgttca ttagagaaac aactcaagaa atcattgata atgcatctgt agtagctatt
    11401 cctacaggaa atcccaatga cgtgaaacag ttaatcgatg tgacagttat agataattag
    11461 actttctaat atgttataca aaaagaagct gacagc
//
LOCUS       DAZKMB010000089        11444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000089 DAZKMB010000000
VERSION     DAZKMB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.089"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..2758)
                     /locus_tag="ACG8V3_002097"
     CDS             complement(56..2758)
                     /locus_tag="ACG8V3_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0300613.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(2771..4060)
                     /locus_tag="ACG8V3_002098"
     CDS             complement(2771..4060)
                     /locus_tag="ACG8V3_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0300614.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            4210..5256
                     /locus_tag="ACG8V3_002099"
     CDS             4210..5256
                     /locus_tag="ACG8V3_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0300615.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            5258..7411
                     /locus_tag="ACG8V3_002100"
     CDS             5258..7411
                     /locus_tag="ACG8V3_002100"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0300616.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(7884..9335)
                     /locus_tag="ACG8V3_002101"
     CDS             complement(7884..9335)
                     /locus_tag="ACG8V3_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0300617.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(9332..11056)
                     /locus_tag="ACG8V3_002102"
     CDS             complement(9332..11056)
                     /locus_tag="ACG8V3_002102"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0300618.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(11049..>11444)
                     /locus_tag="ACG8V3_002103"
     CDS             complement(11049..>11444)
                     /locus_tag="ACG8V3_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0300619.1"
                     /translation="NLLFSLFLSGTALLSYNLYLSVQIKGLIFLAIDFILLFALLCLF
                     ATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSGIILGVTWQFKGLILF
                     GVIGLLTVWNGTMTTHWREELDKQLESYE"
BASE COUNT         3225 a         2301 c         2008 g         3910 t
ORIGIN      
        1 tctaaaattt ctccaccatc tgaaaccgca cacacaaatt ttgttccgcc tgcttctatt
       61 ccgccataca ttcgttttcc tcctcgaatc cgattgtccg aatctcataa ggttggatta
      121 ttgggacaac cgcttgcttc gttggttctt caagcagatt caatatgaca gtttttccat
      181 tgtctttagt tatttccaat ttttctaaat gactagacat attgaaacct cttactacta
      241 ctttatcact aaacttgctg cgtttcaaag ctgtaattgc aaacgtttca ctttttatcg
      301 ttaaatattg ttgttttgaa atcaatgttc cttcatgatg tttgatttgt tgagtggaaa
      361 aaggaatctg tgctgcgtat gcatgcaaat aagtagagaa tttttcttct ggtccatgta
      421 gttcaatgct gtaattgaac cgatgctcac ctagacactg tgcttctggt gttggaaaat
      481 atccccaatc gcctagttct cccacactgc gaagtagcgt cactgcgatt tgtccgtctt
      541 ctgttacttc atattcattc aaaccaaagt ttccgactgt tacaccgtac tcttcatcat
      601 gaatattgac aaatgcttgc tgatgttgcg gattcgtggg gttttcccaa cttttagaca
      661 catggtttgg gcgcttcacc acttcaaaaa tactatctgc ttcgtgatgt tccacatgta
      721 actttgtagg aaacaagaca cgtaagcgat gatctttcat ctgattgtcg agagtcgttt
      781 caaagtcgat ttttttacta tcttttctga ttgtcatgat tgtttttatt tgaaggattc
      841 gtttttcttt ggatcgttct gcttttcgat aacgaaattc cacaaccatc tgctgttctt
      901 tttctaaacg ctcgtctgca gaaacaggga tttccatttc ctgtagaata cttatttttg
      961 ctatttccgg tgtatctact aatactttta cttcagagtt ttctttgtta ctggatagga
     1021 ttggctgatc acagaaaggt tgtttaaaga tatactcatt tcctatatcg ccgatatctt
     1081 caaaaatatg cagattattc aactttcgtt tgtttttgcg atcttccatt gtaatggtac
     1141 catttttttc aatcgtcaaa tgtagagggc cgttttcgat gattcgtcca ctctgatgaa
     1201 tcattgtttc cttttcttct gctttcgctt ccccttctac aagcgcgaat gtttcccaag
     1261 aaaatgctgg catgttttct aatggtaaag tcactttgat cattcttgcc atatatggca
     1321 cacggaaacg gtctttcggc aaatcataac caaattggac agtttcttca gataaaatag
     1381 ctggtatagt ggccccactt tttgtttcta catgatactt acgtttaggc aaattttcta
     1441 actcttgata tagttgttca ggtattcctt cagcgaatct ttttctttct aattcgatgg
     1501 ttatttctgc ttcaccagtt ttatccattc ctgcagtatt tacaatgaca aatggaaagc
     1561 tgtcttttgg gaatccggtc gtatcgattg ctgcggtcag ctgttctaag gaatcttgtg
     1621 caagatactt tccaacttca tctgcttttt caaaacgagg tatcatttcc cgatggacag
     1681 aatcgactga acaaccgcaa atactgtcgt gaggatgatt ttgcattaat gtcttccaag
     1741 cataatctaa ttgatcatgg ggatagtttc cactaacttc ataagccatc gtcgcaagtg
     1801 gttctgtaat attttctagc tgtcgctgga ccttggtatt ccattgtttt aagtaaaccc
     1861 gagcagatgc tgtattggct aaagtatacc agccatccgt ttcttgactt gtcaattctc
     1921 cttctactga gcctaagtct tctggcacat ctttttgaac tgcttccaga tagtcagtaa
     1981 aattagaatg aataaactcg taatctggaa acagttcatt tgcgagatgg attgcttttg
     2041 atatatcttt ttgtaccggt tgatgatcaa caccattcat catcaataag tggttcgtag
     2101 aggcatattg ttctgcatcc gctaattttt gcttccaaaa ggcaagagct gcttcttttt
     2161 ctactggaat ctcattcccg ttgctatacc aattggcaaa tagcagacca aagattgctg
     2221 tttgatcagg tcctttccac cacatctctg agtattggga agaatagttt tctgcttcca
     2281 aaacttgatt atcaaagcca attggtttta caccacggcc aaacgctgct gctgaaatac
     2341 cagcttgttt cattaattga ggcgtctgtc ccatatttcc aaatgtatcg ggaaaatagc
     2401 ctaacatgac aggggttccc catttacggc tttcttccat gccaatcagc atatttctca
     2461 cgttcgattc cgagctgata agaaaatcat cttgcaagat ataaaatggt ccaattctca
     2521 attttccttc attgattgct tgttggattg cctgtctttt ttctggcctt acttgaagat
     2581 agtcatctaa aataatcgtt tgcccatcca aatgaaaact gttgaaggac ggatcaactt
     2641 caaatagctc tagtagatcg tctatcaatt caatcagtcg catgtgatgc tgttcgtaag
     2701 ccatgtacca ttcacgatcc cagtggctat gagaaatgat atatactttc tttttcattt
     2761 acgatacctc ctacttttct actctaatat caaagtaatc catgactaat tcgcaaaaca
     2821 tcatatttgc ccaagaaaac cattctctcg tataattttc aggattgttc acatcgaatc
     2881 cttcgtgcat aaggtgtgtt cctgcgtcag ttgctacaag taaattcaat atccgctctt
     2941 tttcagattt gtctgacgtt gtcattcctt ccatagctaa tgcaatcggc caaacgtatt
     3001 tttctggcgt atgggaggat ccaattcctt ttgcgtactt tccttcatag aaatatggat
     3061 tttctttact caacaatgtt cttcttgttt gcagataatc ttcctcttgt tcagaacaat
     3121 atcccaagta aggtgcggcg atcagattgg gcacattact gtcatccatg actgtggcgt
     3181 ttcccattcc atctacttca tatgcataaa tcgtttgccc tttttgatta accgttcgtc
     3241 cgtgattctg aatcccctct tggatctctt tcttcaattt ctgtgtttgc tgtacaattt
     3301 ctttatcatc gagtatcgtt tgaaaaattt cttccacata acctaacacg accaccgcaa
     3361 acatattgga tggtaccaga tatccataac ggcaagtatc atcgctagga cggaaaccag
     3421 accatgtcat tcctgtaaaa gctgtttttg cacctcgccc gtcattgatc agcgtatctt
     3481 cttttcttgt cgtgtctcgt acaaatgtat aaggagagtg ttcatgatct tgctctgttt
     3541 tgaacacgtc caagattttt ttgatacctt caataaaaga ttgattgaat tgatcagttt
     3601 ttcctgtatt tttgtaaaga agataggcca actgtattgg gtagcataaa gaatcaattt
     3661 catattttct ttcccagatc caatcgttca tttttgtatg atctgtttgg tggccagcac
     3721 cgttagcttc ttcgttgaag gcattcgcat atggatcgat attgatataa aaaaattgct
     3781 ttttgactaa cccgctgatc atgtcagcta attcctcatc ttcttttgca atcactaaat
     3841 aaggacgaac ttgtgcagta gagtctctta accacatcgc tggaatgtct cccgtcaata
     3901 aaaaggtggt tccgtcattt tgctttttga ctgttgtcaa cagagtattg gcaaatgctg
     3961 ctgtaaaatt ctttgcccag tccgcatgga tatccccaca tttttgtgtg atatcttcca
     4021 taaatttctg caccgatttt ggtatatctt tataagccac taataaaacc ttctttctat
     4081 ttgatatatc tttaaagatt atacttcata cagccaatta tgtctacgct ttcaaaaaat
     4141 atctttttat ttactttttc atccattatg gtattatttg aatatgatat atcattaaga
     4201 ggtgtttata tgaatcaacc attgtaccag acaatattta atgatttgca aacggctatt
     4261 ttaactggtg aattacctgt agacagccaa ctaccaactg aaaaagaact ttcacgtacc
     4321 tataaagtaa gcagaatcac ttccaaacgt gcattgactg aattagagca attaggactt
     4381 atttatcgcg ttcgcgggaa aggcagtttc gttaaatccg cttctccgga acagattctt
     4441 gcttctccta caaaaacacg acggattctg tttcttcttc catatgtcgc tgatttgtct
     4501 gttggtgatt ttagcaaggg attgaaccct gtgatgcaag ataaacagat tgatgtcatg
     4561 atgacgacgc ttgactttct acaaaataaa acagcaaaag agctaatcaa tgaatttgac
     4621 ggattgattt attatgctct tgatacagaa caacatcttg acttactttt tgagctttca
     4681 ttgaaagaat tccctgtcgt catattagat aaaaaaatct atgagttacc ttttcctacc
     4741 gttctttccg ataatttcca aggcggtgcg cttgctgcct cttatttgat cgaacaggga
     4801 cattcaaaaa tcgcttattt gtttggtacg cagactcatc cacaatccgt tcgacagcgt
     4861 taccttggtt accttgaagc attggacaaa gctggtttga ctttccacac ttcactggat
     4921 gacaaaagcg cgacattaga tgaattgctt tcttatatca ctaagtatga agtaactgct
     4981 tttgtttgtg aaaacgatgt tgtcgctatc cagaccatga atctcttgag acagcatggt
     5041 tatcatatcc ctgaagattt ttcaattatc ggatttgatg atatccaagc agcagctctt
     5101 gttgacccgc cactgacaac catcgcacaa gattttgaac agttaggtaa gcttgcggga
     5161 gatgctttgg tcacttggtt agagacaaaa gatcgaccat ccgatagtaa atttccagtt
     5221 tcattcatca aaagacaatc aacaaaggag aaataaaatg aatatcggtc aaatagatac
     5281 tagacatgca acagcgaatc agccaaattt ctctaatggg aactgccagc cttatactgg
     5341 cgttcctttt gggatgaatt attttgcacc gcaaactacg gatcaaaacg gcagctggtg
     5401 gtttcatcct gatgaccgcg tttttcaagg ctaccgtctt acccatcaac caagtccttg
     5461 gatgggtgat ttcagtcata tgctgtttac gccgatcaat ggaaaactac aggagaacac
     5521 ccttttccat gctcaaagtt cttaccgccc agaagaaagt atattttgcc cgactcatct
     5581 gtcgatctgt caattacgtt atggcatctg ttctacattg atacctagca tgtatggcgg
     5641 aatcttatct attgattaca gcaaaaatga cagcggttta cttttatcct ttccgggacg
     5701 ccatcagctc tttgttatag atccttacac agtggaagga aaaatcacac attttgctgg
     5761 ttcagaagat cctgattttt ctttttattt tatattgaga tttgaatatc ctttaacaat
     5821 tgatgagtta gctgtttcca acaaaaatag cgactcgatc ccgttgtatt ttgaacagac
     5881 aaaaaaacaa actatccgat ttgggacatc ttttatcagc tcggaacaag cagcttataa
     5941 cctggaacta gaacaaaacc aaagcaaaga gacttacctt gctcacagtc aagaacaatg
     6001 gcagtcctat tttgatcgta tacagctcac acaccatgat caacaacaat tgcggacgtt
     6061 ttatcataat ttatatcggg tgtttttatt tccacaaaca ttttatgaaa ttgatcctag
     6121 tgggcaaaaa atccactatg atacgactag tcgatctata aaacctggtg ttctctatac
     6181 aaataatggg ttttgggata cgtataaaac ggtttatcct ctttattctt tgattgctgt
     6241 agaaaaatac gaagaaatgc tggaaggttt tttgaacagc tatagagaaa gtggctattt
     6301 gccaaaatgg ctgtctcctg atgaacgtgg acttatgcct gggacattga tcgatgcggt
     6361 cattgcagat gctgctgtaa aagatatccg acgagacttg atgcctgaat ttctaacggc
     6421 aatggaaaaa ggtgctacgg ttcaaagcaa acatccaaat tatggacgcc aaggaacaga
     6481 agattacttg acatatggct atgttcctaa tcactaccac gaatcagtca atcacacatt
     6541 agactatgct tatagcgatt tttgtattag ccaagttgcc aaggtactag aagaagagga
     6601 aatgacgaag aaatatcagc agcaatcatt gaactatcaa aatatctttg actatgagtc
     6661 aggatttatg cgttcaaaag ataaagatgg caagttcaga gaaccatttt cagctaatcg
     6721 ttggggacaa gattatgcag aaggaagtgc ttggcaaagc agctttgctg tttatcacga
     6781 tttttcagga ctgatcggaa aatatggtgg cagtgagaga tataaagaaa aactcattga
     6841 gctgtgcaat cagcccccaa cgtttgaccc aggaggatat ggttttgaga tccacgaaat
     6901 gagtgagatg gccgcgatag aatttggaca attagctatt tcaaaccagc caagttttca
     6961 ctatccttac ttgttcagtt atataggaca acctgaaatg gcacagccac tgcttaaaca
     7021 gttgatgact cagaccttcg acgattctcc aatgggttac cctggagatg aggacaatgg
     7081 aagtatggcc gcttggtata tcttcaatag tctgggattt tatcctgtca cgcctggaac
     7141 cggtgaatat gttatcggta tgccattaat tgacaaggct gttttgcttt tgtcaaatgg
     7201 aaaggaattg acgattgaag caacgcctaa tcggccgcag caacaattta tccataaaat
     7261 caaacggatg gacaaagaat atacgaaatt atattttacc catcaagatt tgctgaaagg
     7321 cggcacgatc tcttaccaat tagggatcat ccctcaccct aagaaatatc aagcaaaaga
     7381 tttgcctttt tccttatcaa actataatta acaagaggtt gtgaaaaagc tgtcctgcat
     7441 caagtaataa gaacagccaa gcaaaaatag atcctcatat ttttcgtttg gttggtctta
     7501 ttaccgcaga tgcaagcttt tgaacaccgt ttattgagag gttgtgaaat cagcgttctg
     7561 acctgagtat tagagagaca aatggcaaaa tagttcctca tattttgcca ttttttgagc
     7621 taataccgag ggtcagctga ttgaacaccg tttagtggag aggtcgtgaa aaagctgttt
     7681 agtttcgagt attaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg
     7741 gcttaatacc gagaaactgg cttttgaact ccgtttatcg agaggtcatg aaaaagctgc
     7801 cttgcctcaa gtaataagaa cagcccaaaa ttagatttct agacaatttt ttataagtta
     7861 tttcattaca ttggcttttt cttttatgat tctccactgt tttctcggta ctctttaggg
     7921 gatctgccga cgattttctt gaataattta gagaaatacg tggtgttgtt gtagccaatc
     7981 tcacttgcta tctcattgat gttttgatta ctgtgcaaaa gcagttgttt cgctttttcc
     8041 atacgtaaat ggttcaaata agctgaaaaa ctttttttcg tttctttttt gaacagctgt
     8101 cctagataca tgacatttaa atgcaggttt tctgacactt ctttgagtgt aagtggttct
     8161 tgataatgct ggtgcaggat tacgattact ttttccacat tgtcactgta ctttttctgt
     8221 ctctgatgct ttttgatgaa tgacagaagt agctgctgaa gttcttgcac cgtttttgcc
     8281 tgatggattt tttcgattcc ttgtaaatat tcctcatctt ccagatcaat caattcccgt
     8341 tgaatctcca taaacagtaa aaaactggaa tatcgtatat cttccggcat catggctgct
     8401 aactgaaact gttcaaaaaa atcagtgatc attttttgag cgacttctaa ttgtttattt
     8461 tgcagcacgc gtctgaaact tgaaaaatcg atggtttgat cttttgtttc tgcttgtact
     8521 gcgtaaaaaa catgctcgtc tttatctcca taaaattgat gcaactgtag attgtctttc
     8581 gcttgttgga atgtttcagg gattttgtcc aaagaatctg tctcttcccc gatactgatc
     8641 agccattcct gatcaggaaa gttttcttga acaaaacaac agaagccatc aacttcatct
     8701 gcctccaaca acaacgtgat caatacacgg tctccgtaat ttcgttgata gtaaaagatt
     8761 tgttgccgtt cttgcaacca tctattgata agagtatcat gagttcttgc cagttgaatc
     8821 aatactatcc tatgctgctt ctcacttatt tgtcctatta tttcttcttc acttatttca
     8881 tccaattctt cattcaacca ttgactgaat aacagttctt gatagatttc ttgttgattt
     8941 ttttgttcat tttgctgatc taaccgtgtt ttgattttct tgaggctttc aaccaattcg
     9001 aacttattca cgggtttcat caaataattc acggcgccta attgcatacc tcctttcaaa
     9061 taatcgaatt cttggtatcc tgataaaatc ataaattcaa aattatgacg ttctttttga
     9121 gccgcttcaa taaattccag tccatttatt tctggcattg tcacatccgt tactactaaa
     9181 tcaatctgtc gctgttcaag tacagctagt ccttctttgg cattctctgc tgttgctact
     9241 atttgaaagc ctaaactttg ccaatcaatt atctttttta acccattcaa aatcatgtat
     9301 tcatcatcta ccaataacac ttgatacacg ctcatccctc cttgaatgta atagaaacgg
     9361 tcagtccacc tgtctcatta ttctcaagcg acatataata actgctgccg aagctggcac
     9421 gtaaacgttc attcacgttt tgcagaccga tagaaccgtg aagttccact ttaggatgag
     9481 acagtttttc ttcaatctgt tttagccgtt tttcagtcat cccttttcca ttatctttga
     9541 tgatgatcct tacccgtttg ccttcctgca agactttgac acttagcgca ttatcgaatc
     9601 gagtaaaatc aataccgtgt ttaaaataat tttcaactaa tggctggatc acaaatttag
     9661 gtacttcgat cttttccaaa tcaggatcga tcataaaatg ataggctact cgattaggat
     9721 aacgcatttg atacaaatag acatattttt cgcaaaagtc cagttcttct tttaaagtga
     9781 tcgttttctc ttgattcgta ttgtttcgaa gcaaagcgga aaacgcatac acaacatctg
     9841 ctaattcttc acttccttcg ctgattgcat acattcggat atattctaat gtattatata
     9901 aaaagtgtgg attgatctgt gcttgaagcg cccgcatatg tgcgtcttgc tgtttgattt
     9961 ctaatttgta gatgtcttct acaaactgat tgatgctatc caacatttcg ttgataccga
    10021 ttgaaagttc cttcagttca tattccgtgt ttgttatgtc gatccgcgtc tctaaattcc
    10081 ctgctgctgt ttgatccatg gcctgcatga cgatagatac ttgctgtgaa tatcgtttga
    10141 atgttttata aagaaagata agcagtatat tgtctaaaat aatgatacca atgatcagcg
    10201 gggcaagatt ttttcccgtt tcgattttta cacttttttt atctagtaca gctaatatac
    10261 gataatcatc agacagttct tggtgttgaa caaaattgtt tttttccaga tcgtcaatcg
    10321 gcaattgtga atttttcttc agactctctg tgatgatcat ttcttgtctt tcaaacgttt
    10381 ctttagctat tttattatca tggaatgtgt atagtcgatt tgtcgttcca gaaatcatgt
    10441 ataaagagag tccattgaat gacgttagat tttctaaaat catatctagt tctttctttt
    10501 gaaaaccaat aaaaatactt cctgactcag ctcctccttg tgtgataggc aacttgatat
    10561 aaaaggcgtc tagtaatttt ggtgtcccct tttttatttc cccacctttg ttttctctag
    10621 aagattcata atactccggt agttgattca acacgatata tatcgcactt agttcttcat
    10681 aatttgcgta gagatctttg atttgatttg ggaaagaaac taattcatcg gtactgtttt
    10741 gctgttcgta tacatacatc agatactgat ctatgggttt tgttaagtag gtcgtcacat
    10801 tttcgatctt ttcggaattc cctacaagct ggtttttgat gatcctactc aaggttgttt
    10861 tatcattcag cattctgctt gtgctttgaa cggcatcact tgtcatgttt tctgcctgtt
    10921 tttgattttg ttcccaagta tggatactaa aaataacggt agcagtcatc gttacaccga
    10981 tcatgataac cgcgtatttc cgcaacagac gatttagtaa ggttcctttt gaaaatagaa
    11041 tccgaaactt attcataact ttccaactgt ttatctaatt cttcacgcca atgtgttgtc
    11101 atcgtaccat tccaaaccgt caaaagacca atgacaccaa acaggatcaa tcctttgaat
    11161 tgccaagtca cgcctaaaat tataccgcta ccaataatta tttttagaaa agacgaaaag
    11221 ctggaaaaaa ccgaaataaa acttagcttt agcagattag gaacagagat cgtatattca
    11281 ctatctaaaa tcatactgta ttgataagta gcaaataggc agagcaaggc aaaaagtaaa
    11341 ataaaatcaa tagctaaaaa aatgagccct ttgatttgaa cagataaata taaattgtaa
    11401 ctcaataaag cagtaccaga taaaaataaa ctaaatagca gatt
//
LOCUS       DAZKMB010000090        11300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000090 DAZKMB010000000
VERSION     DAZKMB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.090"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACG8V3_002104"
     tRNA            12..84
                     /locus_tag="ACG8V3_002104"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACG8V3_002105"
     tRNA            88..160
                     /locus_tag="ACG8V3_002105"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACG8V3_002106"
     tRNA            168..249
                     /locus_tag="ACG8V3_002106"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACG8V3_002107"
     tRNA            267..339
                     /locus_tag="ACG8V3_002107"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACG8V3_002108"
     tRNA            351..422
                     /locus_tag="ACG8V3_002108"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACG8V3_002109"
     tRNA            439..524
                     /locus_tag="ACG8V3_002109"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACG8V3_002110"
     tRNA            535..608
                     /locus_tag="ACG8V3_002110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACG8V3_002111"
     tRNA            618..691
                     /locus_tag="ACG8V3_002111"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACG8V3_002112"
     tRNA            711..783
                     /locus_tag="ACG8V3_002112"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACG8V3_002113"
     tRNA            811..884
                     /locus_tag="ACG8V3_002113"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACG8V3_002114"
     tRNA            898..971
                     /locus_tag="ACG8V3_002114"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACG8V3_002115"
     tRNA            995..1084
                     /locus_tag="ACG8V3_002115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACG8V3_002116"
     tRNA            1097..1170
                     /locus_tag="ACG8V3_002116"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACG8V3_002117"
     tRNA            1189..1264
                     /locus_tag="ACG8V3_002117"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACG8V3_002118"
     tRNA            1266..1336
                     /locus_tag="ACG8V3_002118"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACG8V3_002119"
     tRNA            1350..1423
                     /locus_tag="ACG8V3_002119"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACG8V3_002120"
     tRNA            1448..1535
                     /locus_tag="ACG8V3_002120"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACG8V3_002121"
     tRNA            1557..1628
                     /locus_tag="ACG8V3_002121"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACG8V3_002122"
     tRNA            1651..1740
                     /locus_tag="ACG8V3_002122"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACG8V3_002123"
     tRNA            1753..1826
                     /locus_tag="ACG8V3_002123"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACG8V3_002124"
     tRNA            1845..1917
                     /locus_tag="ACG8V3_002124"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACG8V3_002125"
     tRNA            1921..1993
                     /locus_tag="ACG8V3_002125"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACG8V3_002126"
     tRNA            1998..2068
                     /locus_tag="ACG8V3_002126"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACG8V3_002127"
     tRNA            2081..2154
                     /locus_tag="ACG8V3_002127"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACG8V3_002128"
     tRNA            2166..2237
                     /locus_tag="ACG8V3_002128"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACG8V3_002129"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACG8V3_002129"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0300620.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACG8V3_002130"
     CDS             3826..5298
                     /locus_tag="ACG8V3_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0300621.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACG8V3_002131"
     CDS             5381..6169
                     /locus_tag="ACG8V3_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0300622.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACG8V3_002132"
     CDS             complement(6236..6904)
                     /locus_tag="ACG8V3_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0300623.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACG8V3_002133"
     CDS             7019..8731
                     /locus_tag="ACG8V3_002133"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300624.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACG8V3_002134"
     CDS             8734..10506
                     /locus_tag="ACG8V3_002134"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300625.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>11300)
                     /locus_tag="ACG8V3_002135"
     CDS             complement(10588..>11300)
                     /locus_tag="ACG8V3_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0300626.1"
                     /translation="TGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSK
                     EEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYE
                     LKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAY
                     NQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTH
                     TTKNMQNQLVEKLGKIEI"
BASE COUNT         3252 a         2024 c         2555 g         3469 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat
    10921 ttctttagca ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt
    10981 tttaattttt aactcgtaat cataagtacc agaaaccgta attttattac catcataatc
    11041 agattcagtc aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt
    11101 aattaaagaa taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac
    11161 ttcttctttt gataaataac catctttttc tttttttatt tcttcgtttg tcttaggttt
    11221 tcttgctaaa gaaaccccat ttacgggatt atattttaat ccatatcgac ggttggcgaa
    11281 tttaaaaatc attcctgtaa
//
LOCUS       DAZKMB010000091        11197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000091 DAZKMB010000000
VERSION     DAZKMB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.091"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG8V3_002136"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG8V3_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0300627.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG8V3_002137"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG8V3_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0300628.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACG8V3_002138"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACG8V3_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0300629.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG8V3_002139"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG8V3_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0300630.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG8V3_002140"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG8V3_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0300631.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACG8V3_002141"
     CDS             complement(6343..7062)
                     /locus_tag="ACG8V3_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0300632.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACG8V3_002142"
     CDS             complement(7059..8372)
                     /locus_tag="ACG8V3_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0300633.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACG8V3_002143"
     CDS             complement(8453..8773)
                     /locus_tag="ACG8V3_002143"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0300634.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACG8V3_002144"
     CDS             complement(8881..9168)
                     /locus_tag="ACG8V3_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300635.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACG8V3_002145"
     CDS             9295..9939
                     /locus_tag="ACG8V3_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0300636.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACG8V3_002146"
     CDS             complement(9998..10705)
                     /locus_tag="ACG8V3_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0300637.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACG8V3_002147"
     CDS             complement(10925..11095)
                     /locus_tag="ACG8V3_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300638.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1956 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaac
//
LOCUS       DAZKMB010000092        11161 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000092 DAZKMB010000000
VERSION     DAZKMB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.092"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..1382
                     /gene="lysA"
                     /locus_tag="ACG8V3_002148"
     CDS             123..1382
                     /gene="lysA"
                     /locus_tag="ACG8V3_002148"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0300639.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1369..2169
                     /gene="dapF"
                     /locus_tag="ACG8V3_002149"
     CDS             1369..2169
                     /gene="dapF"
                     /locus_tag="ACG8V3_002149"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0300640.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2172..3053
                     /gene="dapA"
                     /locus_tag="ACG8V3_002150"
     CDS             2172..3053
                     /gene="dapA"
                     /locus_tag="ACG8V3_002150"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0300641.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3050..3766
                     /gene="dapB"
                     /locus_tag="ACG8V3_002151"
     CDS             3050..3766
                     /gene="dapB"
                     /locus_tag="ACG8V3_002151"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0300642.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3772..4467
                     /gene="dapD"
                     /locus_tag="ACG8V3_002152"
     CDS             3772..4467
                     /gene="dapD"
                     /locus_tag="ACG8V3_002152"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0300643.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4483..5484
                     /locus_tag="ACG8V3_002153"
     CDS             4483..5484
                     /locus_tag="ACG8V3_002153"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0300644.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5481..6842
                     /locus_tag="ACG8V3_002154"
     CDS             5481..6842
                     /locus_tag="ACG8V3_002154"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0300645.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6802..7953
                     /locus_tag="ACG8V3_002155"
     CDS             6802..7953
                     /locus_tag="ACG8V3_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0300646.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7928..9097
                     /locus_tag="ACG8V3_002156"
     CDS             7928..9097
                     /locus_tag="ACG8V3_002156"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0300647.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9272..10651
                     /locus_tag="ACG8V3_002157"
     CDS             9272..10651
                     /locus_tag="ACG8V3_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0300648.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10932..11161
                     /locus_tag="ACG8V3_002158"
                     /pseudo
     CDS             10932..11161
                     /locus_tag="ACG8V3_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3718 a         1949 c         2497 g         2997 t
ORIGIN      
        1 atatttcgaa atatactaga caattcagaa aattttgttt aatattattc caataaaatt
       61 caaatcttta tcaaatgaaa ttccaataaa aacggaaaaa aagtgaaaag aggaatgaaa
      121 aaatgacaga attgataatt ggcggagtca cagcaagcac attggcagaa aagtataaga
      181 cacctctgta tgtgtatgat gaagaaaaga tgagacaaac gatgctgtca ttcaagcgag
      241 gctttgtatc gaaacgtttt gatacaaaag tgctttacgc ctctaaagca ttccaaacga
      301 ttgagatgct gcatttagtt cgtgaatatg gattcgggct agatgttgtc agtggtgggg
      361 aaatctatac cgctttgcaa gctgattttc ctagagatac tatctacttc catggtaaca
      421 acaagacgcc agaggaactg atctatgcac ttgaaaatga tttgttgcat attgttgccg
      481 ataatttgat ggaagttgaa ttattagcag aattaagcca acgctattta aaaaaaatct
      541 gtatcatgct tcggttgaat gtcggagtag aagcacatac tcatgaatat atcgtcactg
      601 cgcatattga ttcaaaattc ggcatgagct atgaaagtaa agagtgccaa caagcccttc
      661 agttgataaa agaaagcagc tatctagagt tagaaggatt tcatgctcat atcgggtcac
      721 aaatttttga tgttactgct tggttagctg aaatcgacaa attgacgaat tacttaaccg
      781 attttgacca acctctttcc ttgaatcttg gcggagggtt tgggattcgt tatacacaaa
      841 atgatcagcc gatcccagtc gaagaggcac taaaaaaact gattgaatat acggaagaag
      901 ctttggaaaa acgaaaactg accattcgtc aattactgat cgaaccgggt agaagtattg
      961 ttggagaagc tggtacaaca ctatacacga ttggtttcat caaaaagaca ccacataaaa
     1021 aatattactt tgtagatggc gggatgacag ataatatccg tccagctctg tatcaggcag
     1081 aatacgattg tgatttagca gcaaaactgt cggaagaaaa aacagagaaa gtgacgattg
     1141 caggaaaaat gtgtgaatca ggcgatgtgg taattaaaga agcttatttg ccgaaagcag
     1201 aatcgggtga tcttttagct gtttattcca cgggagctta tggatactcg atgagcagca
     1261 actacaatcg agcgacacga ccagcagttg tttttgtcaa taatggccaa tcaagactga
     1321 ttgttcgcag acaagcattt tctgatctac taagggggga agtttcctat gatccttcat
     1381 aaatataatg gctgcggaaa tgattttatc ttattagatt acgcagaaga cacagattat
     1441 agtcaattag ccgcttcttt atgcacaaaa gaaaaatatg atatagacgg attgatcgcc
     1501 gtaaaaatcg atccgttgga aatgatctat tacaacaaag atggcagccg cgcgccgatg
     1561 tgcggaaatg gcattcgctg ttttgctcga tacgtaaatg atcagaacat gatgaatgaa
     1621 caggaattca acgtagagac attagcaggc acaagaaaaa ttacggtgtt ggatccctca
     1681 cctttttatt gcagcgtaga tatgggagtg ccagatgatt ctccggaaaa aatcaaagct
     1741 aatcaaactt atccaataaa aaaccaggaa atagagatca atggtgaaaa agtaacgatc
     1801 actagtttat ttttaggaac gattcatacc gttgtttttg ttgaaaatgc gcgtgaggaa
     1861 gtctggagaa aaagaggtga actgatctgt catcatcctt tgttcaaaga aaaaaccaat
     1921 gtcaactttg tagaagtagt caatgaacaa gaattgatcg tccgtaccta tgaaagagga
     1981 gtaggttgga cacttgcttg cggtacaggt tgctgtgcct cttatgtggt tgctaaggaa
     2041 actgggaaaa tttcagcaga tcaggtagtt gttcacttgg aacaaggaga cttgatgatc
     2101 tctggtgagg atgcgataca aatggctggc ccagcagttc atgaatggac aagaatattg
     2161 gaggaatgaa gatgataaaa ggttcgatcg tggcgctgat cacgccgatg aatgaagagg
     2221 gatcagtaga ttatgctggt ttggaaaaat tgatccaatt ccatttagat gaacaaacag
     2281 atggattatt agttttaggt acaacaggag aatcttctac gctcacacaa tctgaggagg
     2341 agcagatcct tcaactgaca gtaaagaaag tggctggccg tgtgccggtg attgcaggag
     2401 ctggaacgaa taatacgaaa gaaacgattg aaaaagcgaa gcattttgct tctttggggg
     2461 cagacgcact tttggtcatc accccttact acaacaaaac aagtgatgca ggactagctg
     2521 ctcattttac agcaattgca gaagcctcac cgattccgct tattttgtat aatgttcctt
     2581 ctagaacagg catgtcgatt ccaatccatg tattagtaaa tttagcagaa catcctaata
     2641 tcattggttt gaaagaagcc tcaggagata tggcttatgt gatggatgcc gctcggttga
     2701 ttggagaaga attcttcctt tacagtggaa atgatgattt gatcctacca gtaatgagtg
     2761 taggaggaag cggtgtaata agtgtatggg cgaatatcca acctaaaata gtccatgaat
     2821 tagtaaaaga tacgcaggac ggaagatggc agcaagcaaa agaaaagcaa ttaaatgcac
     2881 ttgaactgat ccatgccttg ttcagtgaga ccaatcctat tcctgtcaaa gcagccatgt
     2941 cattattaga cttaccttcc ggacctctac gcctgccttt agtttctctt tcaaaagaga
     3001 aaaagaaaca attagctcag cttttgttaa aagaaaggac ggttgaaaaa tgaaaatcgg
     3061 actgattggc agtggacaga tgggaaatag agtcgaagaa gttgcacaag aaagaggaga
     3121 tacggtcctt cttttacgga cggcacctaa agaagcaact acgcttgctt ttacggaact
     3181 gccagaactg cttattgatt tttctcatcc agataattta gagaaaatcc tttcgtatag
     3241 cttatcatat caacttccgt tagtcattgg cacgacaggt tatacacaat cacagttcca
     3301 agaaataaaa gaacatgcca aacatgtacc tgtgatgtat agtgctaatt tttcgttcgg
     3361 catcatggtg atgaatcaac taatcaagca agcagctgca atgttgcaag gctggcagat
     3421 cgaactgatt gaaaaacatc atagtcgtaa aaaagatgca ccatctggta ctgcaaatat
     3481 gctgcttcaa acaatccaag aagtgcaaaa gctgcagccc gtatacaact ggcaagggaa
     3541 aaaaagagaa gaaaaccaaa ttggtgtaca cagtattcgt gcaggttctt tacctggtga
     3601 acatgatgta ttgtttgctg ctactgatga aatatttacg atcaaacatg aatctttttc
     3661 taatcgaatc tttgctgcag gcgcagtaga agcagcggac tggttgaaag atcagtcacc
     3721 aggttattac aaattagaag atttattaat ggacaaaggg gtgtaaaaaa cgtggaagct
     3781 caagaaatca tccgctacat tcaaacggca tctaaaaaga cgcctgtgaa agtctatctg
     3841 aatgtaaccg agccaatcga atttaaagaa agtaaagttt ttggtgaagg aagcagcttc
     3901 gttgtttttg gtgactatga gacgattgct ccggttttag aagctgaatc agaaaaaatc
     3961 attgaaatcg aaatcgaaac tgctgcaaga tacagcgcag ttcctttgct agatattaaa
     4021 aaaattaatg ctcgtatcga accaggtgct attatcagag atcaagtgtc aatcggcaac
     4081 aatgccgtga tcatgatggg agcgattatc aatattggtg cagtaatcgg tgaaaacacg
     4141 atgattgata tgggtgctgt cttaggtgga cgagcgacag tgggcaaaaa ctgccatatt
     4201 ggtgcaggag ctgtcttagc aggtgtgatc gaaccagcat cggccaaacc ggttatcgta
     4261 gaagacggcg tactggtagg tgcgaatgca gtgattgttg aaggtgtcca tatcggaaaa
     4321 gatgcagtcg tggcagcagg agcagtggtt ttagaagatg tggcagcaga aactgttgtc
     4381 ggtggaatac ctgcacgtgt attaaaaata agtgatgatc agacaaaaga aaatacagca
     4441 ttgattgctg cgttacgcga attatagagg agatggagaa aaatgcgcga aatagatacc
     4501 tgtattgtgg gggcaaccgg attagtcggc caaacgatgc tcaaagtgtt agaagaatat
     4561 gattttccag taggacgatt gaaattatta gcttcaaaac gttcagccgg taaagtttgt
     4621 gtattcaaga aaaagaaaca cactgtacag gaattgacag aaacttcatt tgaaggatat
     4681 gatttagcgc tattttctgc aggtgcttct gtatccaaag agtttgcgcc aatcgcacga
     4741 agtcagggac tatttgtcat agataattcc tcagcatggc gggaagattc ggatattcca
     4801 ttaatcgttc cagaggtcaa tctggaagac tatacattga atcggctgat tgcaaatcca
     4861 aattgttcaa ccattcaagc ggtacttccg cttaaagctt tacaagaagc ctttggtatc
     4921 cagcgagtca attattcgac ctatcaagca gtttctggtt ccgggcaaaa aggcattgaa
     4981 gatcttcatc gtacgcaaaa tgacgaggaa ccagttttgt atccttataa tattagccaa
     5041 acggtgattc cggaaatcga tcagcctttg gaaaatgggt atacaaaaga agaacagaaa
     5101 atgataaatg agacccgtaa gattctgcat cagcctgctt tgccagtatc agcaacctgc
     5161 gtacgtgtgc caatcgaaaa tggacatgga gtctctgtgg cagttcaact gcaaaaagag
     5221 tttacgacgg aagaaattag agagtgtctc agccagtttc cagggattca attagaagat
     5281 gatctagcag cacataaata tccgacgagc attcttgcta gaggaacaga tatggtttat
     5341 gttgggagga tcagaaaaga cacttcttta gagaacggat tgttgttcta tacgactgcc
     5401 gataacatac gcaaaggagc cgctgcgaat gccgtacaga ttgctgcagc tttaatgcag
     5461 aaaatagcgg aggtggcaag atgagtaata cgctgaaagt agcgaaattc ggcggaacat
     5521 cgatgtctca tgaagagcaa ttcaaaaaag tccgtacaat tgttctttca gatcccaatc
     5581 gccaagtgat cgttgtttct gctttaggca aacgtacgtc agatgatgac aaagtaacag
     5641 acttacttta tttgatttat gcttaccttc agcatcatgt tgattggaca cccctttgga
     5701 aaaggatttg tgatcgtttc attcaagtaa gagatgttct tgccctgcag ttacctattg
     5761 aagatgaact agcacgcata cagaaacaac tggaagacga aaagataact gaagattatt
     5821 tagtaagtag aggtgaatat ctgactgctc aactgatggc tgagtatctt ggttaccagt
     5881 ttattgacgc aaaagacctg attttctttg actatgatgg gaaaatcgat caggaaaaaa
     5941 cagcacagga aatcacaaaa gcatttaaaa gatatccgaa aatggttgtt ccagggtttt
     6001 acggggcgaa ccctgcagga aaagaaaaat tattgggccg tgggggaagc gacattaccg
     6061 gcgcgatact ggcgaagatg ttgcaagcgg aaagttatga gaattggacg gatgtttcag
     6121 gaatcatgat ggctgatccg cgaatcatcg atcatcctaa aatcatcgag gaaatctctt
     6181 ttagagaact gagagagatg gcttatatgg gggctaacgt tcttcatgaa gcagctgttt
     6241 tcccagttca agaggctgat atccccatcc aaataaaaaa taccaatgat cctttcgctc
     6301 aagggacaaa aatatcgaat cacgaaatag aaaaggaaaa tggactgaca ggaatagctg
     6361 gacgcaagga ttttctttcc atcactttat tcaaacgtca tatgtccgat gaaatcgggt
     6421 ttatctggaa agcaatgagc atttttgcta aacatgagat atcaatcgaa catattcctt
     6481 cagggatcga caatatcgga gtgatcgtct cagcagaagc gattgccgat aagttatttc
     6541 tcatcacgaa agaattaaaa gaaacacttg gcgtggaaga aatcgtcgtg atcgaagact
     6601 tagctttgat ctctgtcgtt gggggtccgc ataaagaatt gattggatta tctgggaaag
     6661 tgttatcgat attgaacaaa ttagaaattc gcacatcgat cttatcacaa ggagcacagg
     6721 agttgaatct gatcatcggt gtaccgaata atcaatatga aacagtagtg aagggtatct
     6781 atgaagggat ggtgaatacg gatgctgaaa gaatatcgaa tggcactcca tcaaattcct
     6841 gaaatcgggt ttcatgaatt caagacaaaa aaatatttat atgaacaagt aaaaaacagc
     6901 gaggccgtta tccatgaaat cggcgaaaca ggactactcc tttattttga taatcagcaa
     6961 gaagaaacga ttgcttttcg aacagatatc gatgcactgc cagtcaaaga agaaacgaat
     7021 ctctcttttg cttctaagca tgaaggttat atgcatgcat gtggtcatga cggacatatg
     7081 gcgatgcttt tagggttgac tgactttata actaaacaca ttgaggatct tatttataat
     7141 gtagtactga tctatcagcc atcagaagaa atttccggtg gcgcacaaag cgttattcag
     7201 tcaggattgt tggataaata tgaagtacaa gcaatatttg gctttcatct atggcctgga
     7261 ctagctaagg gagaagtttt ctctcgacca ggacctttga tggcacaaag cagtgaaaca
     7321 gacattatca ttaaaggcca gtctgcacat attgcttcta gtgaaaaagg aatcgatagt
     7381 ctggaggcag ctgtacgatt tttgaagaat gtttatgatt ttgaccggca attaccggca
     7441 gagtggaatc atctaatgaa atttggccaa atccaaggtg gaacgatccg aaatgtttta
     7501 gctggagaag ttattgtatc cggaagtatc cgttcttaca gttcagatat gcaaaattat
     7561 ttgaagaggg gattagacca tttagcagaa cagtttcact ctacagctac tatttctttt
     7621 cgctataatg acggctatcc agctgtaatc aaccatagag gcttatatgc agcactgtta
     7681 gattcaaaca aactacatga attagatgct ccagttcttc aagccgaaga ttttggcata
     7741 tatacgcaac aatatccatg tgtatttttc tttttaggtg tcggtgatac ccctgcttta
     7801 catgatccga cgttcgattt tgatatggat gttttagaaa atggtgtaga atggtacaaa
     7861 acgattcttt ttacagaaaa tcttcttgta aaaatcgata gcagttcaaa agaggagcga
     7921 gtaaataatg atgaatttat ccaatcgatt tagtcctagg ctttctagaa tagaagtatc
     7981 aaaaatacga caattcgatc agcaaatatc gtcgatacca gatgtgatca aactcaccct
     8041 gggagaaccg gattttccaa ctcctgaaca tgtcaagcaa gcaggaatag cagcgattga
     8101 agaagacttt tctcactata ccggaatgag aggattagag gaactgcggg aagcggcttg
     8161 tatatttcag caacagcgtt atggattaac ttatgatccg caaacggaag ttttgacgac
     8221 agttggtgca acggaagcga ttgcctctgc gttgttatct gttttggaag aaggagacaa
     8281 ggttcttatt ccagcgcctg cttattcagg atatcagcca ttagtggaac tagctggtgc
     8341 tgaattgatc ccgatagata cttctgacac agggtttgtc tgtcagccgg aacaatttga
     8401 acgagcattt gaacaatatg gatcagctgt caaagcagtc attttaaact atcctaacaa
     8461 tcctactggg acgacattgt ctgcaaaaca attgaaagca atcgctgagg ttttaaaaaa
     8521 atatccggta tttgttataa gtgatgaagt atatgctgaa ttgacttata gtggaacgca
     8581 tatgtccata gctacttatc ttccagaaca aacaatcgtg ataagcgggt tatccaagtc
     8641 acatgcgatg actggttggc gagtgggctt tatttttgct caaaaagccc ttattgacga
     8701 actaattaaa gtccatcaat atctggtgac tgccgctaca acgatgtcgc aaaaagctgc
     8761 ggctgaagcg ctaataaatg gggtgaaaga tagcgagaat atgaaagagc aatatcaaac
     8821 acgaagagac tatctggtcc agcaattggc gcctttagga tttgaagtga cccagccaaa
     8881 cggtgcattt tacttgtttt gcaaactccc agctgccatt caaactgact cttggcagtt
     8941 ttgtgtagaa ttagcggaac aggcaaaagt tgcgtgtatc ccaggaattg catttggccc
     9001 agaaggagaa ggttatatcc ggatttctta tgctagcagc atggaaaaat tacatgaggc
     9061 ctgcaaacgg atcaaagact ttttaacaga acagtgattc atagatttta tcaaaaaaac
     9121 aatcgttaat tttgcggtat attctgcaag gagacgattg ttttttaaat ttagttcaaa
     9181 tgaacgcgtt tcttttgatt cataactaat gtaatgttaa gtttaaattg tttagaatga
     9241 ttttaaaaga atattcaagg aggtttcatc aatggaaaaa atcaaagttg tcattgtagg
     9301 tgcatctcat ggagggcatc aatcgatctt ggaactattg acacgatacg gtgaaaatgt
     9361 agatatcacg ctatttgaag caggagacta tatttccttc atgtcatgtg gtatggagct
     9421 gtatttagaa gacaaggtaa cagatgtaaa tgatgtacgt aatttcagac cagaaaactt
     9481 tccgcaatcc aatgttcata ttttaaataa tcacgaagta aaaacgatcg atgccgataa
     9541 aaaaacagta acggttacta gcgcatcaga taacgaaaca aaagaatatc cttatgacaa
     9601 attgattttg agttctggtg tcaaaccaaa caatctgcca gtaccaggag cagatctaga
     9661 aaatgtctac ttgatgcgtg gatacaattg ggcaaccaaa atcaaagaaa aattaaatga
     9721 tccggctatt aaaaaaatcg ctgtcgtagg tgcgggttac attgggatcg aagcagcaga
     9781 agcaagccga aaagctggga aagaagtcgt attgcttgat gtgatcgata gacctttagg
     9841 cacttattta gatgctgaga tgacagatat tttagaacaa cacctaaatg aaaacggtat
     9901 tgaagttttg acaaatgcaa aaatcgaagc ttttactggt aacggaaaag ttgaggcaat
     9961 caaaacaagt gaaaaagaaa tcccagcaga tctagtcatt caagctgccg gtgtgaaggc
    10021 aaatacagaa tggttaaaag ggatcgtcga cttagatgaa cgtggctgga tccaaacaaa
    10081 tgaatacttg caaacgaatc ttccagatgt gtatgcagta ggagatgcta cacttgctta
    10141 ttctatccca gcacgcaaaa aaatgccgat cgctttagcg actgttgcac gtagagaagc
    10201 acgttatgta gtaaaacatc tttttgaaga agtaccaaac aaaccgttcg gcggagtcgt
    10261 aggttcctct gcattaagtg tctttgacta tcattttgca gcaagcggat tgaatagctt
    10321 tacagcagca aaagcaggag tagatgtacg agtgtcttat tatgaagata cgttacgacc
    10381 aaaatatgta ccagaagaac aaggaaatcc aaaagtgtcc atccaattat tctcggatcc
    10441 aatgacccat caattgctag gaggagcagt attatctacc tatgatgtta caggtcaagg
    10501 aaatgtattg gcactagcga tccaaaacaa actgacttta gaagaactag cagaagctga
    10561 cttcttcttc cagccaggct ttgaccgaca atggagcatg ttgaatctag ccgctcaaca
    10621 agcgttagga gaagaaccat ttgtagaata agaaaacgaa atatttgaaa agaggttgtg
    10681 aaaaagctgc cttgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt
    10741 taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta ttttcaaaaa
    10801 agggactgtg acacgatttg tgtaccgacc cccaaaagtt agaccaaaaa atctaacttt
    10861 tggaggtcgg tattttttat ggttaaatat acttttgaac ttaaattaaa gatcgttcat
    10921 gattatttgg atggcaaagg cgattggacc gtaaaaatcc agatcaagag attgaagcgg
    10981 aaatgcttaa aattcgggag aagcacaagg attatggctg tttacggatg accaatgaac
    11041 taagaaaccg cggtttctct atcaataaaa agaaagttca gcgcttgatc aagaagctag
    11101 gaataaaagt aacggcatac acgcgtaaat cacgtcgcta caattcttat cgaggaaaag
    11161 t
//
LOCUS       DAZKMB010000093        11087 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000093 DAZKMB010000000
VERSION     DAZKMB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.093"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..1132
                     /gene="pplA"
                     /locus_tag="ACG8V3_002159"
     CDS             197..1132
                     /gene="pplA"
                     /locus_tag="ACG8V3_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0300649.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1222..2325
                     /locus_tag="ACG8V3_002160"
     CDS             1222..2325
                     /locus_tag="ACG8V3_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0300650.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2338..3291
                     /gene="menA"
                     /locus_tag="ACG8V3_002161"
     CDS             2338..3291
                     /gene="menA"
                     /locus_tag="ACG8V3_002161"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0300651.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(4105..5451)
                     /gene="gor"
                     /locus_tag="ACG8V3_002162"
     CDS             complement(4105..5451)
                     /gene="gor"
                     /locus_tag="ACG8V3_002162"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0300652.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(5585..6838)
                     /locus_tag="ACG8V3_002163"
     CDS             complement(5585..6838)
                     /locus_tag="ACG8V3_002163"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0300653.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(6844..7767)
                     /locus_tag="ACG8V3_002164"
     CDS             complement(6844..7767)
                     /locus_tag="ACG8V3_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0300654.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(7988..10480)
                     /locus_tag="ACG8V3_002165"
     CDS             complement(7988..10480)
                     /locus_tag="ACG8V3_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0300655.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(10487..10954)
                     /locus_tag="ACG8V3_002166"
     CDS             complement(10487..10954)
                     /locus_tag="ACG8V3_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0300656.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         3266 a         2264 c         1942 g         3615 t
ORIGIN      
        1 tttgtctaac tccttttgga tttttttcaa attttaaaat aaagcgtttt ttcacgaaat
       61 ttgctgaaag aaaacgtttg aaatattaca tgggttattt atcaaaaata gttgacttat
      121 aggctaaaaa gattagaatg tataatgaat cacaaagaaa aattgtggac tagaaatgga
      181 atggtggggt attaaaatga agaaaaaacg ttttgtaact ggatttgccg tattagcatt
      241 ttcagctcta gtgttaggcg cttgcggcgc agatgacaac ggaagctcaa actcttcttc
      301 tgaatcatct acagcacaat cttcgactgc aaaaagcaca acagaatcat ctgcaaaagt
      361 tgtagctggc ggagatttgc aagacggcac atataaatta gaagaaaaaa attattcaaa
      421 tggttaccgt gcagtctttg aaatggttgt aaaagacggc aaaatcactg aatcaaaata
      481 tgataacgtc aatgaaaacg gcgaatcaaa aacaaaagat gcagaataca acaaaaacat
      541 ggaagcaaaa tctggcacaa acccagaaaa atacattcct gagttgaatg accaattctt
      601 aaaagcacaa agcgcaagcg gtgtggaagt cgtaactgga gctactcact catctgaaag
      661 cttccaaaac tacgcacaac aattgatcca agctgctcaa gctggtaaca cagatacaat
      721 cgaaatcgac aatggtgctg atttgaaaga cggcacttac aagcttgaag aaaagaacta
      781 ctctaacggc taccgtgtgc aattcgaaat gacagttgct ggcggcaaag taactgaatc
      841 aaactttgat tatatcgaca aagacggtaa atcaaaacaa gatgatacag aatataatga
      901 aaatatgaaa gcaaaaagcg gtacagaacc aaaaacttac atccctactc tgaacgacga
      961 attcgtcaaa gcaatgggcg aagaagatgg ctcacctgct aatgtagaag ttgttacagg
     1021 tgctacacac agctcacatt cattcatcat gtacgcacaa caattagtga atgcagctga
     1081 aaaaggcgac actcaaacaa tcgaagtaga taatatcgta acagaaaaat aatcacataa
     1141 tcatactaat aaaagttgga gggtgggacg atagccagat taggcagtcg tctcaccttt
     1201 caaaatgaag agaggaaaaa gatgcggaaa aaactactat tttccttaat cacactgggc
     1261 atctctttta tggtgctaac tggctgcagc aactcagcaa ataaaacaac cactgaaagt
     1321 tcagcagcta ccaagatatt gaaagatcca tattctgatg aacagttctt gttagggact
     1381 tacgttcgta tccgtgtgta tgatgaaggc aaagaatcag cattgaaacc tgccttcgat
     1441 cgagtaaaag aacttggtga taaaatcacg atcaatcaaa aaggttctga gatcgatgaa
     1501 gtgaatgagc aagctgggat caaaccagta aaagtttctg atgacgtcta cacgttagtc
     1561 aagcgggcct acgaatatag ccaagattct caaggcggat tcgatatggc gattggtgca
     1621 atcacacaat tatggcgaat cggttttgat gacgccagaa aaccttccca agaagaaatc
     1681 gatcaggcat tgaaattagt ggattatcat aagatcgaac taaatgacaa agaaaaaact
     1741 gtttatctaa aagaaaaagg aatgatcatc gatttaggtg caatcgccaa aggttacatt
     1801 acggacgaag tagtgaaagt cttgaaaaaa caaggagtga ccactgccat cgttgacttg
     1861 ggcggtaatg tctatgtctt aggccacagc ccaagaggcg aaaaccaaga ctggacagtc
     1921 ggcatccaag atccgaacat ggctcgcgga tctgttttag gttctattaa agaacggaac
     1981 aaaacattag ttacctctgg aatttacgag cgttatttag aagtagatgg caaaaaatac
     2041 catcatttgt ttgatccaaa aacgggttat ccgttcgaca atgatatcgc aagtgtcacg
     2101 atcataacag ataaatcaat tgatggtgac gggttgtcta cagcagtctt ttcaatgggt
     2161 gtgaaaaaag gactggaata cgtcgaaagc gaattgaata acgggacgga agcgatcttc
     2221 gtgacaaaag acgataaagt atatgtgacc gatccaatca aagacacctt caaattatcg
     2281 gaggactccc attacacgat gggtgaccgt tctgaactaa aataaggagt acgaaccatg
     2341 acactaaaaa tctttttaga agtagttgaa atacgaacaa aggtggctag tgtatttcct
     2401 tttgcggtag gggtattatt ctctatcgct ttttttcatg agatacattg gctaaacact
     2461 tttttatttt ttattggaat gctagttttt gatttagcga ctaccgctat caataattac
     2521 atggattatg aaaaggctca ttctgaagta tataagtatg aagaaaatgt cattggccgc
     2581 agaaatctat ctcctgtatt gattcgaaac atgattttag ctatgattgc atttgttttt
     2641 gttatcggcg ttattctaac gatcaatacg ggttggcttt tacttttgat gggatttatc
     2701 tgttgtttta ttggagtatt ctatacattc ggaccgattc ctttatctcg aatgccttta
     2761 ggggaaattt tcagtggggt gactatgggc cttggaattt ttgccatggt cgtttatgtc
     2821 aatacattcg gttcagatgt ctatgatctg acagtcaact tttcatctgg acaatttgct
     2881 ttggtaggaa atctttgggc tatagcagca atcgtattag cttccttacc cttggtgttt
     2941 acgattgcca atatcatgtt ggctaataat ttgcgggatc ttgaccgtga tattgaaaat
     3001 catcgctata cactagtttt ttatatcgga cgtcctgcgg gaacagtctt attccagcta
     3061 ttgatgtatg cttgctattt cgtgatttta atggggttgt tgagcggtat ctatgaatgg
     3121 ccgatcttgg tcaccttctt gaccttgcca attgtgaaga agcatttgga tcaattcaag
     3181 caaacgttac cccagcctat cagtttcagc tattccatca aaaatatggt cttattcaat
     3241 gccagttatg tattggggct attactaaca atcattatgc aagccattta acagcatgaa
     3301 gaggttgtga aaaaactgcc ctgcatcaag taataagaac agccaaataa aaatagctcc
     3361 ttatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttat
     3421 caggaggttg tgaaaaagtc gcttagtttc gagtattaag aaaaattttg tgaaaatggc
     3481 tttccacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt
     3541 atcagaggtt gtaaaatcag tgttttgtcc tgagtattag agaaacaaat ggaaaaatag
     3601 ttcctcatat tttgccgttt tttgagctaa taccgaagga cagctgattg aacactattt
     3661 gttcggaaaa agtgactaac tatgacacaa tttttttcac agtccctttt ttcacttgat
     3721 ttgattcgta ccagcgaact tgacgagaac tttcttggtt tggtaactat atggtattct
     3781 tttaacatcc tcgtttgctt cttctaattt atttatacaa agtcacactt ttttaaacat
     3841 ctgttcttcg atacatttct atttctttta aacatcgata ccaatatttt gaacttaaga
     3901 gtgcatacca aatggattca tttactcttc gtcagttacg aagataatca aaatctccaa
     3961 cttccctact tatttcgttt tgagaattat cgaagcaatc ttacataaat aactaaaaaa
     4021 caaaaaacca gaaaatcagt atactgattt tctggtttta catggtttgt tcaataaaaa
     4081 gctgttggaa ctgttaatat taaattatcg catcgttacg aattcttccg ctcctgttgg
     4141 atggatggct actgtattgt caaagtcttc tttagttgca cccatcttga tagccacggc
     4201 aaaaccttgc agcatctcat cgacaccaac accaatagca tgtaagccga cgattctttc
     4261 ttcttttccg acacagatca atttcatttc gcatttttgg cgataatcat ttaatgcaaa
     4321 gtacattggg gtaaagcgtg aacgataaac tttcaattca ttttcgccat atttttccaa
     4381 tgcttcttct tctgtcaaac caattgttgc aattggtgga tgcgtaaaga cgactgtcgg
     4441 aacaagttta tagtcaagat aagaatcttt ctttccatta aataaacgtt ctgataaacg
     4501 tctaccagct gcgattgcaa caggagtcag atctaattta ccgatcacat caccaacagc
     4561 aaaaatatgt tcatccgttg tattttggta tttatctact ttgataaacc cttttttgtc
     4621 caattccaca tttgtatttt ccagacctaa tttttcggta ttcggtacac gtccaccggc
     4681 aaaaagtaca caatcaccag ttaaggtttc accattttca aatagcaccg tatattcgtc
     4741 attgttttta gtaatttctt tagcaatatg atgtgcgtaa gtttttattc cagcttcttg
     4801 gtacatttgg atcaaattgt ccgaaagcat tttatcaaac gttctaagtg gtcgttcttt
     4861 tcgatatgcc cacatgactt cactacctaa accacgaaat acacctgaaa gttcagccgc
     4921 aatgtatcca gcacctaaaa cgattgctct cttaggtaac tctgttaatt caaaaaatcc
     4981 atttgaatca attgcgtatt ctccaccagg aatggtcatt ttacttgcac ggcctccagt
     5041 agcgatcaag attttaggag cacgataggt ttcaccgttt acttcgatcg tttgactatc
     5101 gataaaagta gcgtaacctt ttatagcctc gacatgattg ctatctaatc ctctttgata
     5161 agcaccgtgt agaaaatcaa tatatttttc tcgtctttct actagaccgg cgaaatcaac
     5221 attttttaaa tctgcttgga taccgtaact tgaggcatct cgttcaattg tttccaaaat
     5281 cgtacttgct tgccacataa cttttttagg tacacaaccg acgttgacac atgttccgcc
     5341 caattcattc gcttcaatca gcaaaacttt tgcgccatgc atccctgctc tgtttgctga
     5401 agcaataccg ccagagccgc caccaattac gatgtaatca tatatttgca ttttgttcct
     5461 ccttattgaa gagatcaatc aaaaccttta tttaacgatc ctttttctac agaaaacaag
     5521 tgtaacactt ttcttgaatt ttgtatgcga agatgcttaa aacagggata tatcagcttt
     5581 ttccttattt acgttttcgg atcatatctc caggtttcac cggctgtgcg actggcatag
     5641 ttaaaatcat cattggattt ggtgctcggt caatggattc gccttcttca ttatgcataa
     5701 cttcaacggt ttgactgaaa tgactgaatc ctggaccata aaattcgatt tcatctccta
     5761 cagaaaacaa gtttctctga cgtatcgtag caatctgtgt tgcctcatca taagataata
     5821 cttcaccgac aaatttatat actgggattt tacgacgttc accgaataat tgttcgtttt
     5881 cagttggtgt attgtagtaa aaacctgtag ctaattctcg ttgtgctact ttccataact
     5941 cgtcgatcca ttcttgcttg cagacatagt tttcaggatc ttccatataa ctgtctacag
     6001 ccgctttata gacatttgac accgtagata cataatggat cgatttcatt cttccttcga
     6061 tcttgaagct gtctacgccg ttttcaatca attctggaat atgttcgatc attgacatat
     6121 ccactgcact catcgagaat tcttcttcca cttcgcctgt atcagttaaa ctgtttcgtt
     6181 cttgaccaaa aggcatatcg aacaatccat acttccagcg acaagattga gaacatccgc
     6241 cacggtttgc atcccgcatc gacatatggt tggacaatgt gcatcgacca gaataagaga
     6301 tacacatggc accatggata aatgcttcga tttcggtatc cgtattttta cgaatctctg
     6361 ccacttcctc cattgaaact tcacgagcta aaacgacacg ttccaaccct tcttctttcc
     6421 agaattccaa tgtctcaaaa ttagttgctg aagcttgagt ggataaatgg atcggcagac
     6481 ctggggcttc tgtcgcacaa atttcgatca aagctggatc agaaacaatc actgcagaaa
     6541 taccgacatc acgcaaactg cggaaaaatt cccctgctcc atcttcattt ccttcatggg
     6601 tgaccatatt agcagcaacg tataccttcg catcatgttc ttttgcaaaa gcaactcctt
     6661 cagccatttc ttcataagta aaattacctg cacggcttct taaaccatat gcattcccgc
     6721 caatatatac tgcatctgct ccataatgga tcgcggtttt taacttttcc agtgttcctg
     6781 ctggcgctag gatttctggt cgtttcaatt ttctttttac tgtcatttct ttcatcatac
     6841 tccttatttg atagcagaag gatcaatata aaagaatcct gtatctaatt cacggttttc
     6901 aggatgcaat ttactgattt cgctagtcat tttttccgct ttttcttgat cccagctacc
     6961 ttctaaaatt tgtttttttg cttcggcgaa tagtcgagtg atgtttacga atttttcgcc
     7021 tggagtaaat aatccgtcca atttccatgt acagtaatga tgctgataaa gttcattcaa
     7081 ttcattcgag agattcagat cattgctggc aaaaatatgt gtgccatgac tatcttcata
     7141 aatagaataa tgtgtctctt cttttttagg ttccgagata aaaagacctc tttcacgatc
     7201 tttttgttcg tcttgtttcg tatagttata gtaattctgt agcagtggac gtttggattg
     7261 atggatgcat gtagcaccat acacaagggt ttctacagga atatcgagtt tttcttccat
     7321 cgccttcatc tcttcaaaag gaacttcacg agcaagtact gctccaactg cgcctttttt
     7381 cgcccagaaa ttgatttgac gtgcgctcgt tactaatgtt tcagcatcat aaatatacgg
     7441 tatacgcaat tcaggatttt ttttcatcgt ataaataata cctgtatcac caactgtgat
     7501 cacatctacg tttatttctt ttaaaaaagc caaatattca ggtacatttt tcattttttc
     7561 aggatgaatg atcccattta cagcaatatt cgctttttta ccatacttgt gtgctaattc
     7621 taccaattgt cgctgttctt ctcgtgtaaa tgaagcaggc agacgcaatc caaattgctc
     7681 ttcaccgaaa tatagtgtat ctgcacctgc agcaagcaac tgttctgctt gttcaatagt
     7741 ttctactgtg gttatgagtt cgatcataat tttctcctcc tacaacttca taatagtagc
     7801 atagctctaa aaactttcca atacttcttc tatttaaagt cggatttttg aaaattattt
     7861 cacaaaaaat caaaataaag ttcgatatat tctgtccaaa tataaaaaga gattgtgaca
     7921 aaaatcttgt cacaacctct taatcaagtc ttactattat aaaaattcag ctacacaggc
     7981 caagtaacta tagggaacct actgtttcct ttttaggtcc gcgaacattc aatgtgatct
     8041 taccttttga agcacctact gttacgtggt cacctaaacg aatttctccg ccaagcaaag
     8101 cctcgctcaa tcgatcttca atttcttttt gaagtgcgcg acggattgga cgtgcaccat
     8161 attcaggatc aaatccagct ttcccgatca catcgattgc agatggtgtg attttcactt
     8221 gaatatcttg ttctttcaat cgtttgataa tcgctttgct catgatttgg acgattgtat
     8281 ggatttcatc tttactcaat gaatggaaga cgacagtctc atcgattcga ttcaagaatt
     8341 caggtcggaa agctttcttt aactcttcta agatacgttt ttgcatggct ttgtgatctt
     8401 tagtaacatc ttggacattg aatccgacat ttttttcttc tcgaatttgt gtcgctccga
     8461 tatttgaagt cataatcatg attgtatttc taaagtccac ttttctacct ttagaatctg
     8521 ttaaatgacc atcatccaat acttgcagaa gtaaattgaa aacgtctgga tgtgctttct
     8581 ccacttcatc taagagaatc actgaatatg gtttttgacg aattttttct gtcaactgtc
     8641 caccttcatc ataccctacg tatcctggag gagacccgat taatcggctt gtactgtatt
     8701 tttccataaa ttctgacata tcaacacgga tcagggcatc ttcacttcca aacatgactt
     8761 ctgaaagtgc tttggccaat tctgtttttc cgacaccagt tggtccaagg aacatgaatg
     8821 aaccaattgg acgatcagga tctttcaatc cgcttcgtgc acggcggatc gcacgtgaaa
     8881 ctgctttgac agcctcatct tgaccaacga cacgttcatg cagaagtcct tccagttcta
     8941 acaagcgttc gctttctttt ttctccaact gctgtagagg gacaccagtc cactccgata
     9001 caactgtagc aacatcttct tctgttacac gatcagaata accttgctcc tgttttgctt
     9061 caattgcaga aactttttgc agctttttac gtagtgcttt ttcttgtctg cgtaattgag
     9121 cagcattttc aaagtcttgt ttttggatcg ctgcttcctt ttcttgtact agttcatcga
     9181 tttctagttt gatcacagtt gattttgtta agtgatcggt ttgatccaaa cggactttcg
     9241 ctgctgattc atcgatcaaa tcaattgctt tatcgggtag ttgacgagaa gtgatataac
     9301 gaacagaaag attgactgct gctcgtacag cttcatctgt gatctccact ccatggtgct
     9361 cttcatatct tgtacgtaat ccttgaagaa tcacttctgc ttcttcagga gttggctcat
     9421 ctacttgaat gcgagcaaaa cgtcgctcta atgcagaatc tttttcgata tatttttgat
     9481 attcatctag ggtcgttgct ccgatcgttt gtaattctcc tcgagctaac gccggtttca
     9541 agatattcga agcatcaatt gctccttctg ctccacctgc accaattaag gtatgcaatt
     9601 catcaatgaa taagattact tgaccatcat tatagatttc gtcgatgacc tttttcattc
     9661 gatcttcaaa ttcaccgcga tatttcgtac cagcaaccaa agatcccata tcaagcatca
     9721 tcaggcgctt ttcttgcatg tcttctggca cttcacctaa aatgattttt tgagctaacc
     9781 cttcagcaat ggctgttttg cctacaccag gttcacccac cagtacaggg ttattctttg
     9841 tccgtcggct aagaatctgg ataagccgtt tcacttctct cgaacgacca acaactgggt
     9901 ccagcttgtt ttcttttgct aatttcgtca aatcacgtgc taatgaatct agcgtaggtg
     9961 ttccttgcgg tgtttgccgt ttatcttgta catttacatt tctacgacga ttcattcctt
    10021 ttccagcaga atcaccagct agccccattt tttgtttcag aacttgacgg gcttttgaaa
    10081 ggctgatacc taagttgatc atgattcgag tagccatgat actttcatct tttaataagc
    10141 tcatcaaaat atgttctgtt ccaacttttt gagaaccagt tttcttcact tcgctgctag
    10201 ctaaagcaaa aacttgtttc attcttggtg aatatggaag aaagactcct ttagggtaag
    10261 aacggatacc accataacca atcagatgtt caatttcttc ataaatttcc gtttcattca
    10321 aatccatttc acgtaatact tttccagcaa ttccattttg ctcgaccact aaggctagta
    10381 aaacatgttc tgaactaact gattgatgtt tcaaccgttt tgcttcttct tgtgcaattt
    10441 ctaatactgc ttgggctctt tcagtaaaaa gttcttccat ctaatctcac ttcccatctt
    10501 cataacttaa acgttccaga aatgctcgga gaatccgagc acgtgtatga tcttcatatt
    10561 cattgaaatt caatgtgttt tttgcaattg cactcaacat caagtttcct tctttttttg
    10621 taattatttg gtcttcataa agtttttgaa taatagcaaa agcatttttc tcactgattg
    10681 tatcatcaaa taactgattt atctgctcaa gtaattgttt tttatctgaa atccttactt
    10741 ttgcaattcg tatataacca ccaccaccac gtttgctttc tactagataa ccacgttgga
    10801 tggtaaaacg ggtattaatc acatagttga tttgtgaagg aacacagtta aagaggtctg
    10861 ccatttcaat ccttcgaatc tcgatcattt cactattttc cagaattttc tttatatatg
    10921 attcaattaa atccgaggta ttctgatgtg ccatcttatt cattcccttt gactaatatt
    10981 gaccttaatt ataacgaatt tgataataga aagaaagaag aacgcttttt gttaaataaa
    11041 tcctttagaa tcaacaaaaa aaaatacgga atatactatt ccgtatc
//
LOCUS       DAZKMB010000094        10897 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000094 DAZKMB010000000
VERSION     DAZKMB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.094"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..696
                     /locus_tag="ACG8V3_002167"
     CDS             118..696
                     /locus_tag="ACG8V3_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0300657.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            696..1625
                     /locus_tag="ACG8V3_002168"
     CDS             696..1625
                     /locus_tag="ACG8V3_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300658.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1622..3358
                     /locus_tag="ACG8V3_002169"
     CDS             1622..3358
                     /locus_tag="ACG8V3_002169"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0300659.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3421..4257)
                     /gene="pheA"
                     /locus_tag="ACG8V3_002170"
     CDS             complement(3421..4257)
                     /gene="pheA"
                     /locus_tag="ACG8V3_002170"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0300660.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4254..4763)
                     /locus_tag="ACG8V3_002171"
     CDS             complement(4254..4763)
                     /locus_tag="ACG8V3_002171"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0300661.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4756..6051)
                     /gene="aroA"
                     /locus_tag="ACG8V3_002172"
     CDS             complement(4756..6051)
                     /gene="aroA"
                     /locus_tag="ACG8V3_002172"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0300662.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6064..7155)
                     /locus_tag="ACG8V3_002173"
     CDS             complement(6064..7155)
                     /locus_tag="ACG8V3_002173"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0300663.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7172..8338)
                     /gene="aroC"
                     /locus_tag="ACG8V3_002174"
     CDS             complement(7172..8338)
                     /gene="aroC"
                     /locus_tag="ACG8V3_002174"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0300664.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8340..9404)
                     /gene="aroB"
                     /locus_tag="ACG8V3_002175"
     CDS             complement(8340..9404)
                     /gene="aroB"
                     /locus_tag="ACG8V3_002175"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0300665.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9437..10459)
                     /gene="aroF"
                     /locus_tag="ACG8V3_002176"
     CDS             complement(9437..10459)
                     /gene="aroF"
                     /locus_tag="ACG8V3_002176"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0300666.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         3028 a         2402 c         2006 g         3461 t
ORIGIN      
        1 agcatctaga ccaaagatcg aaatggtgag gtttctttaa aataaaaata tccttgcatt
       61 ttctcctaga aataagtatt tttaaattac aagactattc ttaaggcggg taaaaaaatg
      121 ccaatacgcg taattcttat cgatgatgaa cagacgattt tagatggaat gaaacagttg
      181 atcaattggt ctgagcacgg gtttcaatta atagggactt tttccagtcc gctgaaagcg
      241 cttgattttt ctttggtaca tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa
      301 ttaaatggaa tcgaattatc gcggctatta aaacagcaac aacctgaaac acagatcctt
      361 atcctctcta gctatgatga atttgatatg gttaaagatt cttttaaaga aggcgtcgct
      421 gattatcttt taaagcctcg attgagtcct gatttttttc taaattcctt aactgccgtt
      481 tcacaaaaaa tcaaaaaaaa gcccaacaaa agcccaatca ccaatcgtta tgagcagatc
      541 tgcgagaatc tttcacagca gattgctgga caaactcgtc cattgcttga ccctcttctt
      601 tttgaacaca ccttgttttt ctgttatata ccgaaaaacg gccattcagt tcaaaacaag
      661 gctctaaaca aaaacagtcg atcacccatt cattgatgga tagcgacgat tcgtttacta
      721 tttatcctgt ttctaccttt tcggaagaat ccggctatgt aatcaatact gattccaaag
      781 atacacttag gaagtttgtt caaaagtttc gtcaagaagt ttttccaggt gatttttttg
      841 ttgtttctga ttcttttgca atccaagata tcaatgaaat attccaagag atcagaaatt
      901 gcaccaaagg acaacgtttc tatcataagg gaaagaacct tgcttatcag catgaacttc
      961 ttccgttatt aaccaaccac gagcagcaag caagtagtta tttaacaaaa atcgttcata
     1021 aagactacgc tttagctgtc aaagaacttg agaatttctt cacgacgata ttggaacagc
     1081 ctattgatcc agcttatttg aaacatgagg caatcaatcg tttctatgca ttactcaacg
     1141 ctttggctga cgagcatgtt gggaatgaag aactttcttt gctcaagatc gcccttcctt
     1201 ctttattagc cgatgctgag tatttcgaag atttttctat tcttgtcaaa gaaacacttg
     1261 agaaactcac ccacttttct gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga
     1321 tctacgagtt cgtccaggaa aattatgaga aagaaatcaa tttacaactt ttatccgaaa
     1381 aatatcattt ttcatatagc tatctctcag ccatctttac tgaaaaattt ggtatcaact
     1441 tttcaaaata tctaaaaaaa gtccgtatca gtaaagccaa gctctttttg acggaaacac
     1501 ggcttacttt gacggaaatc tgtgagaaag tcggctatac cgaactgggt tatttctcca
     1561 gagtttttaa agaagagaca ggaatgacac cttctcaata ccgtaaagga acttttgtaa
     1621 aatgagaaaa aaaatcaaag agcatgaact ctttgccaaa cttctgttca ttttgttcat
     1681 cggtcttttt ctgcaagcca tcgtcatttc tttgtttatt tatcatcgat caagggatgc
     1741 ctatatccag ctgttcaatc aatcgaatga tgtggtacta aaaaagattc aatctgaatt
     1801 tgaaagtttg aatgatacaa tcgagaatac attagccgct tttgattcta atccagctgt
     1861 caaaagctat ttttccaatg atccagccca acatatggaa cagcttcagc aactaagaac
     1921 aatccaaaaa atgaatgact ctctttcaaa aatccatcca atgatcgatt acgatgtatt
     1981 gatctttgga gaaaatggaa gaacttttgt cggaaacgac atgctcactg ccgtatctgc
     2041 cgattctttt tttcaatctg cgattgctca gagagtgaat gaacgtgctg ctgatacaca
     2101 aatgcttttt gcacaccacg gattgactct tcgcgataag aaagcaccca gcgtcttctt
     2161 tgtcaaaaaa ctgaaaaata cattgaatca cgtttacgga tatgctgttt tatcgatctc
     2221 atccaagcaa ctagcgaatc tttttcaatc tgtagtaaat cctgagatat cgaagatctc
     2281 cttcatgaac gaagaacaga cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg
     2341 atcaatgatt ttcaaaaaac taacagtagg tgaaacaacg atttcaaatg gacagcagtt
     2401 gacccgtctt cctctgtacc gccaggattg tacattactc agtcaagtcg atcttcattc
     2461 tttatctaac caaatgggcg tgatttttcc gattattttg tataatatcg tgacacttgc
     2521 ttttgctggc tcattggtat ttatgtattt ggatcgacac acaaaatcga tctaccgctt
     2581 gattgattcg ctaaaacggc tagaggaaga tggcttaaaa gctactgttc ctgtacaagg
     2641 aagccatgaa gtccaattgc tagggaatac gatcaatcaa ttgctaagta atgctcagca
     2701 tcattatgaa cagacgatcc aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat
     2761 gcaagcacag atacagcccc attttattta taatacgctg actagcttga aatttttgat
     2821 ttggcagaaa aaaacagatg aagcagttca aggaatcgac agtttcatta ctttactaag
     2881 aagtacgatt gggaaaaaag aagaagtgat tccagtcaag gaagaaatca aaagcgtaca
     2941 aagttacatc gatcttttga gtatgcgata tggcgaaaga atctcggcta aaataatgat
     3001 tcctgatgaa ctgcagtcat tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa
     3061 cgcctatctt catgcttttc acaataaaac gcaaggattc atcacggtgt acggcaaaac
     3121 aaaaaatcag cttcttttat tcgaagtgat tgataacggc gatggcttcg atactgaaaa
     3181 taccaaacaa aaaaatgatc atttttcagg aatcggcatc agccatgtag atgagcggat
     3241 acgcctaatg tatggcgatg catatggctt aacgatccaa tcagtgattg ggacaggaac
     3301 gactgtaacg atttgtctac cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag
     3361 aatagccaag cgctgatttt tttattttaa gttaaataaa tcaatcagta ttcttgactt
     3421 tcatctacgc gtctcttcta ctgtcagcac tggataacag cccaatattt gactttctcc
     3481 tccgagcatc ttgatttctt ggagcgcatt ctctactaat gtcattggac ggtccaacaa
     3541 caaatcgatc acgaaaaaat attctcccaa gctagttttc aatggtcggg attcaatctt
     3601 acttaagttg atttcccgcc agccaaaagc tgccagcatt ttatgaagca tccctggccg
     3661 attagcaggt agtgtcaaga tgacactcat ttttactgga gctgactgct ggctggtcat
     3721 ctttcggtca ccgacgatcc aaaaacgtgt ctggttcaac tcattatctt ggatattctc
     3781 agctaagatc tccaatccaa catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc
     3841 ttctttcgga tgttctgcta catacattgc tgcagcagtt gttgattctg ttgctactaa
     3901 aggaacatta ggataatgcg tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg
     3961 tgaaaggatt tttgtaatct ttgttgctgg attgcctaaa agctgctgct tgataggaag
     4021 aacgatttca cttttaactt cgacttctgg atgtttggaa agaagatcga ttgtgtgatg
     4081 aacagctcct tccaatgaat tctctacagg aacgacagcc agatcgactt gtttgcgaaa
     4141 caaagcgcgc aaacaaacag ggatggaagc atacgcagtc aattgttctg taggaaatag
     4201 ctgacaagcc gcctgataag tgaatgaact ttctggccct agatagctga cgttcatatg
     4261 cttttgatcc tttctataat ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg
     4321 attcgatgtg tcgcactttc ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt
     4381 tcttctcttg tttttccaag agctaaaggt cgaatagacg tttcatcttg ttggatacga
     4441 tcaactagac agtccgcttc tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa
     4501 cgccgatttt ctgcactttg gacaatccct ccaccagtcg cgataatttt gtcttcattt
     4561 gacagctttc ttaaaatatc tgactccact tttcgaaagc ttttttcacc gtaatagtga
     4621 aaatattcgg caatcgacaa ttggatatgt tcctcaatct ttgtatctaa atctacatac
     4681 tctttttgta aacattgagc taacccttta ccgattgttg tttttccagc gcccatgaaa
     4741 ccaatcagca aaatgctagc catgagcttc tcctcctgac acaagttctg ctaaatcgtc
     4801 aaaaaatgcc ggataagaga ttgaaacagc ttcagctcct tctagttcgc aaggtccatc
     4861 agctatcaaa gcagctactt gcagcatcat accgattcga tgatcgcctc tgctggacac
     4921 ttttgcgcca tgcagaggtg tcggcccatg gatgatcaga ccgtctgctg tcgcttttat
     4981 ctttgctccc attttctgta attcttctgc caccgcatca attctgttgg tttcttttac
     5041 ttttaattct tctgcgtctt tgatgatcgt gattccttct gcttgagtag ccactaaagc
     5101 tagaatgggc aattcatcaa tcagacgcgg aatgatttct ccaccaatcg ttgctttctt
     5161 caaatgactc gtcttgacac ttaaatcagc cgattggttc tgttcattgt gattcgtctg
     5221 ggtaatcgct gcgcccatct cctccaacac atctaagata ccagttcttg ttggattgac
     5281 cccgacattt tttaacaaca gttggctttc cggaacaaga agtcctgctg ctagaaagaa
     5341 agcagctgat gaaatatcac ctggaacagt tatctgttga ccagttaatt tttgcggtcc
     5401 ggtcactatg atcgttttat cttccactgt gatccttccg ccaaattgac gaatcatttc
     5461 ttccgtatgg ttacgggaac gttctttttc aacgattacg gatgtgcctt ctgcttgtaa
     5521 tgccgcaaac agcaaagcag atttgacttg cgcactggct acaggcatat tgtatcggat
     5581 aggtgataaa gagcgggttc ctgtcacagt gattggtaga tactggtcat tttcttctcc
     5641 ttgcaagtca gcgcccatct cgcgtaaagg ttccatcact cgccccattg gacgtttaga
     5701 caacgaagca tccccgaaca gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa
     5761 taaacgtatg gtcgtaccgg agtttcccat gtcaattgat tcggttggtt ggacaaatga
     5821 gtggctaccg cgaccatgaa tcttgatcac ctcttcctct tcttcgatct cgacacctaa
     5881 ttgttggaag gcatgtaaag tactcagaca atcttctgcg cgcaaaaaat tttgtatagt
     5941 cgtcgttcct tcagcaattg ctccaaacat gatgctgcga tgggaaattg atttatctgc
     6001 tggtatccta acagtccctc ttaatccatg tatttgttgt aataattgca tttatccagc
     6061 ctcctatttg atccagcaat gataactggt attagacata atgattcttt ttgcttgtaa
     6121 caaatcctgt tggtttttaa aactgatttg cagtattcct aaaatatctt ccctggtctc
     6181 ttggatcttc aaattaatca aagaaactcc cgcttttccg atcagtccag ttacttctgc
     6241 aataacgcca ggttcatctg gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc
     6301 ttgcttatgg acaggcaact ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat
     6361 ttccgattgg ttctcagtca tgatccattc tgctatttgt gtcatttctt gttgccaatc
     6421 tgccaatcgt tccaataata tctgcttatt gctcaacaaa atatctgtcc acatgacagg
     6481 atctgaggaa gcgatcctag tgatatctcg aaagcctcct gctgccagtt gtttggctcg
     6541 cggatactct tgattgaatt gttctgcctg attgactaag ccagctgcca gaatatgtgg
     6601 aaaatgactt agcattcctg ttatacggtc atgttctctt gctgtaagag ttacatattt
     6661 tgctttggtt ccgcaaaata gttcttgcag tttttcaaca tcttttttcg tcttttccaa
     6721 aggtgtaaag atgtaatagg cattttcaaa tagtttagca tctgctgcct gtatacctga
     6781 tttatgagaa ccagccattg gatgtccacc gacaaaccga agatttttct gctcagcgga
     6841 tgtcacgact tgctgcttcg tactgctgac atccgtaatg atgacgtgat cagacaagtt
     6901 gattctttca aggacatcaa gataagctaa ggttgtacgg acgggaacgg ctaataagat
     6961 aacgtctgct tgttctgcac ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg
     7021 ttctactgct tgctcacggg tttttgactg actatcccaa ccaactacta tcagttctgg
     7081 attggtctga cgaatacaaa gtgccataga agcaccaatc agtccaagcc ccacgataaa
     7141 aacatgttgt gccatcttat ttgccccctt tttagtaatg tttcaagtag tcacggtact
     7201 ccttcactgc agctttcaac tcaacaagcg aatcacttgg gaattttgac aatagctctg
     7261 tcgctaccac agtggctaca acattttccg caacgacgct agctgcagga actgctgtac
     7321 tatccgaacg ttctacactt gctttgtaag gttcttttgt atcgatatca acactttgaa
     7381 gtggcttgta caatgtagga atgggtttca tgaccccgcg aatgacaagt ggttcgccat
     7441 tggtcattcc gccttcgaag ccgcctagat tattggtacg tcggaaatag cctctttctt
     7501 cttcccatag gatctcatcc atcacttctg agcctggtaa tgaaccagaa gtgaatccga
     7561 ccccaaattc tacgccttta aaggcattga tactaaccac agcttgtgcg attttggcat
     7621 ctagcttcgt gtcccattgt acgtagcttc ctaatccagc tggaacgcct ccaacgagca
     7681 cttctacaat tcctccgatc gtatctccat ttttctttgt ctgatcgatc aaatctttta
     7741 ttctttgctc aactgctgga tcaacaactc gtacttctga cgcttcagaa atttctttga
     7801 tttcttgaat cgagatattt ccaggtattt ctgcttggat tccaccaaga caaatcacgt
     7861 gtcctgctac gatgatgtca agttcttcca gaattttctt ggcaattgcg ccgattgcta
     7921 cacgcatggt cgtctctctt gcagaggacc gttcaagcac attccgaaga tctcgatgat
     7981 ggtatttcat accgcctacc aaatccgcgt gtcctggacg cggacgtgca actcgccgca
     8041 gtgattggtc tttttcaggc acttctgcta cagacatgac agttgtccaa tttttccaat
     8101 ctttattttc taccactaaa gtgacaggtg aaccaattgt tttgccgtga cggatgccag
     8161 acgtgatccg aacacgatct ttttcgatct tcatccttcc tcctctgccg taaccagctt
     8221 gtctgcgagc gagttcgtga ttgatttctt cttctgacag aggtaatcct gctggtagtc
     8281 cttcaataat ggctgttaat tctggtccat gggattctcc cgcagtcata taacgcatct
     8341 tacagtcctc ctaagtaatc tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc
     8401 ccaatctttt ctagcaagat gatattcaat tggcttcctc ttgctttctt atcatgggtt
     8461 atcgcgtgga ataattcatc tggatgactt agatcgtacg taatcggcag atggaatttt
     8521 tgaagtatcc tttctaaagc agctgtcgat ccagttggag acaagccgat tttttctgca
     8581 tgagaagtga tggctaccat tccaatagct actccttctc catgactgac taccccatat
     8641 cctgctgtat tttcaattgc atggccaatt gtatgaccaa aatttaaaat aagtcgattt
     8701 ccttggtcaa agacatcttc ttccactact tttcgtttga tttccaacgt agcagtaatg
     8761 attggaacag catgttccag aagatctttt tcgtctttca gctcgttcag ctgtttccaa
     8821 agcgttgcat ctgctatagc tgctgatttg atgatttcag cgattccttc tcgtaaacga
     8881 cgtaccggca aagtttttag aacctcagga tcaatcaata caccagctgg ctggctaaat
     8941 gttccgacaa ggtttttcgc tttgtttgta ttgactgctg tcttcccgcc aatactagaa
     9001 tctacttgtg caagtagcgt agtcggaatt tgaacgaaat ggatcccacg catgaacgtc
     9061 gaggccacga atgctgctag atcacccacc attcctccac caagtgcaag tatcccatcg
     9121 ctgcgggtaa aatcattatc tgctaaaaaa tcgtataact cagctgcttg ttctaaagat
     9181 ttactggtct cacccgccgg tacgattgaa aggactggtt caaaattagc ttgtctcaat
     9241 tggaacatga ttttttctgc atatagtgga gcaacgtttg tatcagtgat cacagctact
     9301 ttttgaggtc tccaaatcgt ttgaagccat gatccagcct gttctagtgc gcctttttca
     9361 atatgaatat cgtatttttg gttatcaaca tgtacagtaa gcattcgttt cacttctttc
     9421 ttttaaaaag ctggaattaa tcgagtgtct gaatcttttc cattgcttct ttcatgatcg
     9481 caacttcttt catcatgcgt gagtaagtct tttcattcaa cgattgggga ccgtctgacc
     9541 aagcatttgc aggatcagga tggatctcta cgatcatgcc atctgctccg cttgccacac
     9601 ccgcacgagc cattggtggt acaagttccc aaattcctgt accatgactt ggatcaacaa
     9661 tcacagggaa atgagttaat tttttcatca atgggacagc acttaaatca aacgtattac
     9721 gtgtcgctgt ttcgtaagtc cgaatcccac gttcgataaa aatgacagga gatttgtctt
     9781 cgactgcaat gtattctgca gcattaagcc attcattgat cgtaccggaa attccacgtt
     9841 tcaatccaac aggtttacct gttttaccga cagcagatag cagtttaaaa ttttgcatat
     9901 tacgtgcgcc aatctgtagg atatcgctgt actcagccac catatcgata tgcccttcat
     9961 ccatcacttc agtaatcact ttcattccaa attcatcagc cgcttgacgg atgtatttca
    10021 atccttcttc ttctaaacct tggaaagcat atggagaagt acgtggtttg aaggcaccac
    10081 ctcgcagaat ttttgcgccc ccggctttgg ccattcgcgc acattctctg atttggtcta
    10141 atccttcgat agaacaagga ccagccatcg tcacgaaact gccgtcacca atttttactc
    10201 cgtctacatc cacgactgta ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt
    10261 ttaaaatacg cacagcattt tctactcctt catagcgtgt aaatgctcct tcttgtaaac
    10321 ttcgcgtatc accttttagt ccaatcacta ctcgttctgt tccttgatcg attgagactt
    10381 tgaatccttc tttttcgata tgatcgacga ctttttttat ttgttcttcc gttgcttctt
    10441 ttttcataat tactatcatt gtttttcccg ctttcttttg tttacttatc tatcttggct
    10501 gattttctct actttttcaa ggtggtttta cattaattcc tactaaatga ccgtgtcctg
    10561 ttgttctcat tttcgctcct tctttcctac ttatgaccca gataatcaac aaaaaaagat
    10621 gtccctgtag gaaattgcct ctacagtgaa catcttcaac gagccttaaa aagcgctgca
    10681 aaaatataaa atagccacta tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa
    10741 atctttagcc atcatagata caaacctggt ttgtttgtac ccacgaagtc atgaatacaa
    10801 acctatctaa aatagctaaa gtaatacgta ttcagttttg taagttttct agatttcata
    10861 aaaaatcgct ccaaactgat gtaagaattg tacctag
//
LOCUS       DAZKMB010000095        10825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000095 DAZKMB010000000
VERSION     DAZKMB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.095"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8V3_002177"
     CDS             476..1339
                     /locus_tag="ACG8V3_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0300667.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8V3_002178"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8V3_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0300668.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8V3_002179"
     CDS             3339..3860
                     /locus_tag="ACG8V3_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0300669.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8V3_002180"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8V3_002180"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0300670.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8V3_002181"
     CDS             5941..6759
                     /locus_tag="ACG8V3_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0300671.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8V3_002182"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8V3_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300672.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8V3_002183"
     CDS             7228..7806
                     /locus_tag="ACG8V3_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0300673.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8V3_002184"
     CDS             7901..8038
                     /locus_tag="ACG8V3_002184"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300674.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="ACG8V3_002185"
     CDS             complement(8851..9354)
                     /locus_tag="ACG8V3_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0300675.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="ACG8V3_002186"
     CDS             9713..10738
                     /locus_tag="ACG8V3_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0300676.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT         3308 a         1884 c         2107 g         3526 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagc
//
LOCUS       DAZKMB010000096        10800 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000096 DAZKMB010000000
VERSION     DAZKMB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.096"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG8V3_002187"
     CDS             85..624
                     /locus_tag="ACG8V3_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300677.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            897..2069
                     /locus_tag="ACG8V3_002188"
     CDS             897..2069
                     /locus_tag="ACG8V3_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0300678.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            2325..2525
                     /locus_tag="ACG8V3_002189"
                     /pseudo
     CDS             2325..2525
                     /locus_tag="ACG8V3_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3237..3842)
                     /locus_tag="ACG8V3_002190"
     CDS             complement(3237..3842)
                     /locus_tag="ACG8V3_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0300679.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            4024..4359
                     /locus_tag="ACG8V3_002191"
                     /pseudo
     CDS             4024..4359
                     /locus_tag="ACG8V3_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            4524..4739
                     /locus_tag="ACG8V3_002192"
                     /pseudo
     CDS             4524..4739
                     /locus_tag="ACG8V3_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4877..5848
                     /locus_tag="ACG8V3_002193"
     CDS             4877..5848
                     /locus_tag="ACG8V3_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0300680.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            5988..7301
                     /locus_tag="ACG8V3_002194"
     CDS             5988..7301
                     /locus_tag="ACG8V3_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFQ0300681.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            7344..8210
                     /locus_tag="ACG8V3_002195"
     CDS             7344..8210
                     /locus_tag="ACG8V3_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0300682.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            8207..9055
                     /locus_tag="ACG8V3_002196"
     CDS             8207..9055
                     /locus_tag="ACG8V3_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0300683.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            9069..10541
                     /locus_tag="ACG8V3_002197"
     CDS             9069..10541
                     /locus_tag="ACG8V3_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0300684.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            10558..>10800
                     /locus_tag="ACG8V3_002198"
     CDS             10558..>10800
                     /locus_tag="ACG8V3_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0300685.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         3484 a         1837 c         2206 g         3273 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttgata aagtccatat aattgtgtaa aagataaaag gccatgtaac
      841 agccctttta cggtacaatg tttttaacca caaaaacata cccaggagga cgttacatga
      901 cccaagtaca ttttacactg aacaacgaag aggttcaaag tattattgaa cattcggtaa
      961 aagatgatgt ttctaaaaat attttaacca ctgttttcaa ccaattgatg gaaaatcaac
     1021 gaacagaata tattcaagcc gatgactatg aacgttcaga aagtcgtcag agtcaaagaa
     1081 atggctatta tgagcgagac tttacgactc gtgtgggtac actcgaatta aaagtgccta
     1141 gaacacgtga tggtgaattt tcaccgacgg tgtttgagcg ttatcagcga aatgaaaagg
     1201 cactgctcgc ttcaatgctt gagatgtatg tttcgggcgt ttcgacacgt aaagtttcaa
     1261 agattgttga agagctatgt ggaaaatctg tatcgaaatc ttttgtttct agcctgactg
     1321 agcagttaga cccgatggtc aacgaatggc agaaccgttc actctcaggt acgaattatc
     1381 cttatctgat gactgatgtt ctctacataa aagtccgtga ggaccatcga gtgctttcta
     1441 aaagctgcca tattgcgatc gggataacag aaggtggcga ccgtgaaatc attggcttca
     1501 tgattcaaaa tgaagaaagt gatgacacat ggtccatctt ctttgaatac ttaaaagaac
     1561 gcggcctaaa ggggacagaa ctcatcattt ctgatgccca taaaggccta gtgtctgcga
     1621 ttcgtaagtc atttaccaac gcaagttggc agagatgcca ggtccatttt ttaagaaaca
     1681 tcttcagttc cattccaaaa aagaattcaa aaccgtttag agaagcagta aaagcgatct
     1741 ttaagtttac ggatattgaa ctcgctcgaa cagctaagaa tgccttagtc ggtgaatata
     1801 tcgaccagcc taaatataca aaagcctgcg aaatattgga taatggcttc gaagatgcct
     1861 ttcaatacac ggttatcgga aatagtcata atcggctaaa aagcaccaac cttcttgaac
     1921 gactgaacca ggaagtccgc agaagagaaa agattattcg gatttttccc aaccgaacgt
     1981 ctgccaatcg attaattgga gctgtcctta tggaccttca tgacgaatgg ctcagttcta
     2041 caagaaaata tattaagttt gatcaatgag agaccggtaa aacattgtat agtattttac
     2101 acaggattat ggacttgact tgagaacacc acgcatagtt tttattttga gatattctag
     2161 aatgaaacac attaagaacg aaagcaaagc aaaagacgcc agaacttcta ctaccttcta
     2221 agttttttca tatttgtttg ttatgctata catagatctg cataattaga cttgcgctgc
     2281 ccgttattga agtttcaggc agcgctcttt ttgggggatc gataatggaa gaagaaaaaa
     2341 tatttgagaa acgctggcag ctagcatcaa gtgaacaaag agcaagatac aataatttga
     2401 tcacctccta ccccactgtt gactagacat ataaggaaaa gaaatactta ctttggctgt
     2461 gccagttaga tatagatacc tttgaaacat ttgaagtgat cctagataaa attaaacaga
     2521 attgatcaga aacaagcgaa gccttctctc ggttcgcttg ttttgctatt tattagttgg
     2581 ctcagggcaa ctctaagctc gtttgaaaga aaaccgatgg ataatcccct tgtttgtacc
     2641 actttagaat acatggatgt acgtattgcc attgactcct attagttact gaaaaaaaat
     2701 ttaaacaagt tgagtattgc agataaatag ctccagtgag gtgaataact gctccactca
     2761 aggcaataga tagctccatc cagggcaaca aactgctcca ctttcatatt taatagatga
     2821 ataaccttct gtataataga aaggcatacc tttcggtatg acaacttata cagaatgtta
     2881 ttttattggt tcactatcga aaaattttgg agctttattt ttccggtatt tctcaacgaa
     2941 cgattagttt aactgtggga agctccagac atacgattcg agaagttact tctcgagcag
     3001 acaggaaggg ggtgactgaa ctaaccgctg atatgagcga tacttggctg taggaatatc
     3061 tttttccgga aaaacaaaca acagcacgag gttaccttcc tgtagactgg gaatatgttc
     3121 ataaagaact aatgaaaaag aatatgacat tgaaactcct tcatagagaa tatggggtac
     3181 atggaacata gaaacgaaaa tgcacggtag aaaagcgaat gaatatagta taaattctac
     3241 aaatcattcg ctttctttat tttatatatg gttggtcgag agagaccatt ttctttggca
     3301 atttctgcaa ctggaactcc ttgatttaac atgtccacca cagttttaaa gactaatcgt
     3361 ttttgcggat ctgttgagtt ctcagaatat tcaattggtc gccccttata tcgaccagct
     3421 tttttggcca ataatactcc ttgccgctga cgttcacgga ttttctcacg ttcgttttgt
     3481 gcaatataac ttagcaatga taaaaacata tcgaacatag cagtatctag gagttcattt
     3541 ccagtattaa aatctaaaaa ctgtgcatct agtattttta atgctacttt tttctgtttc
     3601 ataaaggtaa ctgtattttt tatgtcttca taatctctgc ctaggcgatc cagtgatgca
     3661 acaatgacac aatcaccttc tcggatataa tttagtaatt tttgaaactc aggtcgctgg
     3721 atatctttac cactggcttt ttcttggaat aatttatcaa tgttaaggtc ttccatcatt
     3781 gccaattgtc gatctaaatt ttgatcttgt gaagacactc taatatatcc aatttttgcc
     3841 ataaaaactc cttttaaaaa atgatataat ttacatgtaa aatttacttg tagttttact
     3901 gttatcatag catttttaaa aataaatttt aagtctactt ataatttact ttggaggttt
     3961 tgaatgaatg aaattaagcg caataaaaat tcttttggtg tttatttagg agagcctagc
     4021 tcggaacagt taaataaagg tgaagctcgt catgctctat gtcgaaatat attttatggt
     4081 agaaaaggca aactttatca aacttattac gacggtatgg aggaacagct caacgcatta
     4141 ggattagtaa ctaatgctat catatattgg aattcactat atttagaaaa agtctttgaa
     4201 caaattaaaa cggaagggtt cgactgttcc gaagaagtgt tacgcaagca atttccatta
     4261 attagtgatc acattaattt tgttggcaaa tatagtttcc gctatgaaaa agaattatcg
     4321 aatggaatac tacgtccgtt aaatatagag aattattgag aatgttcgtc ttttggttcc
     4381 taccgtgcat tttcgcacgg atgttccaga tactattttt gaatgtataa aattgaaaac
     4441 acaagaatac taataaatca acgattcttg tgtttttaat ttttcactac cgtgcatttt
     4501 cgtttctatg ttccatgtac cccaatcttg aaaaattaat taaagactta gataaagcca
     4561 ttgacgactt agtcgtggtg ctaccggagt atcaatgttt aaccagtatc ccaggtattg
     4621 gtcctgttta ggctgctgga atactcgctg aaattggtcc aattgaacgt ttttctgatg
     4681 aaataaaatt gacaaagtat gccggcctat attggcgaca gagtcaatcc ggtaaatctg
     4741 aatacgaaaa tttttttacg aaaagcttca cgtctaatat ctattttaaa ataaaaatat
     4801 tcctgctaat ttggaatcgt ttccttcttt tggtataata atacctgaaa ttcttttgag
     4861 aggagttgtt cgtgaagtga aaactactat gaaggatgtt gctgcgttag caggagttgg
     4921 tgtgggtacg gtatctagag taataaatgg agtaaaggtg aaagaggtta ccaaagaaaa
     4981 agtaatggct gcaattagtg agcttaatta tgagcctgat gaatatgcca gaggtatgaa
     5041 aacgaatagt agcaatacaa tagctcttat tttacctacg atttggcatc ccttcttttc
     5101 cgagtttgcc tactatgtag aggaagctct cagccaaaaa aattataagc tttttttgtg
     5161 taatgcgaac ggcagtgcgg aaaaagaaaa agaatatatc caaatggtca agcagagtaa
     5221 agtagacgga attataggta ttacctattc agacattgat aaatatgtat catcaaattt
     5281 accatttgtc agtatagacc gccatttttc ggaacaggtt tcttatgtaa cggcagataa
     5341 ttatttaggt gggcaaattg ccgctcaaga actacttact cgtaactgta aaaacttgtg
     5401 ctatgttggg ggtgtttcac cttaccctaa cgaaacaaac aatcgtaaag ctggttttta
     5461 caatatttgt gaggaaaata aaatagctgt gaaggtattg gatatgcctg aaccaattag
     5521 caatttagag gaacaattgc tcgctttttt ttcggataat cctgaaattg atagcatttt
     5581 taccatcaat gatttcatgg ctttggatgt gataaaagcc caaaaaaaac tagggaagga
     5641 agcacctaag gattttcaaa ttattggttt tgatggagtc aagctatcgt tgcaaaaaga
     5701 ctatttacta tcaacgattg tacagccgat agaggaaatg gccaaggttt cagtagaaac
     5761 actcatggcg atgattttaa atgaagaacc gcctaagcgt gtggtagtac cggttttctt
     5821 tcacgctgga gagacgacta aagagtaaaa aaccggttta taacgatttt tttgagatag
     5881 gttgacagaa agtgatttca atgatatctt aaaaccaatt aattggaaac gttaccaatt
     5941 aattgaaaaa taaaacggaa acgtttcaaa aaataggagg aacatcgatg aaaaagagaa
     6001 tgttagcaat tgcagcaatt ttagcattaa cagctggagg acttgcagga tgtggcagta
     6061 aggagaaaag ttcaggtagc agtgatccag gagaaattaa gatttgggtt cagttttctg
     6121 atgaaacgcc tgaaggaaaa gcttgggcag agattgtgaa caattttaat gatgaatacg
     6181 agggtgaata taaagctgtt actgagtata ttcctcgtag cggaagcggc ggtggatatg
     6241 aggataaagt aaatgctgca attacaacca atacccttcc tgatgttatt acgttagatg
     6301 gaccgaatac tgcagcttat gctcattcag gagtcattgc tccattagat gattatttga
     6361 aagatgcgga catggatgat gtattagaca gcattattca gcaaggaacc tacgatggca
     6421 aaatgtatgc gtttggtttc tcagagtcca gtgtgggtgt ctattacaat aagtcgctgt
     6481 tcaaagatgc tggaattaca gaagatcaat tgccgacgtt agaagatcct tggacttgga
     6541 cggaatttca agcaatttgc caaaagctga ctgataaata caaaacgcct gcaattgata
     6601 tgcaaatggc gtcgggagac gaaatgctga cctacgcata tacaccattt atatggtcga
     6661 atggcggcga tgtcgttaat gctgatggaa ccaaagccga agactatttt aacggtaaaa
     6721 aatccgttga agcgttggaa tttattcaag aattggttga aaaaggttac accacaatca
     6781 gtcctattga aaaaggattt gaaactggca aatatgccat gatgctgagc ggttcttgga
     6841 caatggcaga tttagaggaa aactatcaag agattgatta tggtattttg ccttaccccg
     6901 tttcagatgc aactggggag ttagtttcac caaccggaag ctggcagtta gcaatgactg
     6961 agaaaacaga gaaaaaggat gcttctgcgg cctttattaa gtttgcaacc agtacagaat
     7021 ctagtcgtat tatgagttta gggaacagtg ttctacctat cagaaattca acagttgatt
     7081 taattaaaga tgaagtgtcg gaaggaatgc aggtattaat taagcaaaac caactttctg
     7141 gacatgcccg cccagtggtt gtagcttatc cgcaagtatc tagagcattc caacaagcag
     7201 ttcaagatat tagctacttc gaggaaaacc cagatgtaca aaaagtagca gatagtcgcg
     7261 ctgctgaaat gcaacaggca attgatactt cactaaaata gaagtttcta aagggggcca
     7321 atcaagtgtt aaaaaaaccg acaatgaaag acaatttgat aggttacggc tttctagcgc
     7381 cagctttgct gctgctgacg atttttctga tcatacccgt aatcatggta atttattatg
     7441 cctttactga ttattatttg ttaacgccag atgctagaca atttatcggc ttagaaaatt
     7501 ttaagcgact gttcgctgat ccaatttttg taaaaagtat ttggaacacg gctaaatttg
     7561 ttgtttggat tattccggtc caaatagggg cagctttagg aatggcactt attatcaaca
     7621 aaaatcgtaa aggaaatatc ttttttaaag tagccttttt tgcgccagtc gtgatgtcct
     7681 tagtcgttat ttcgatttta tggttgtact tgttaaatcc taatgaaggt ctggttaata
     7741 cgctatttgc aaagattgga attgcttcgc agcctttttt gactagtccc aaacaagcaa
     7801 tgtatacgct tgttttcgta tcggcatggc aaggtgcagg atatcagatg ctgctgtttt
     7861 tagggggaat gcaaaatatt ccgcaagacg tttatgaggc agccgaaatt gatggttttt
     7921 ctaagtttca gcaatttcgc tatatcacca tgccattatt aaagcccacg gcgattttta
     7981 ttttacttac cacattgatt tcggccttta aactaatcat tcagccaatg gtaatgactc
     8041 aaggtggacc aatgaattct actatgacaa tggtttatta catttaccaa actggattta
     8101 cagaccgtat ggtaggctac tccagctcca ttgcgttaat ctttaccact atgattggtt
     8161 taattacaat tgctcaaaga aaattggtaa aggaggatga tccagcatga ccaagaaatt
     8221 tggaccgcta atgatcgtag aatattgttt gctgttcctt ttagcggcat tattcatttt
     8281 cccgatgctg tggatggtag tttcctctat gaagcctgaa gcagatgtct atcaaaattt
     8341 agcttccttc aaagcttttt tacctagttt gaatccagct aattggttta aaacttatca
     8401 agaggttatt tcccggttca gtgtgggaac ttatttatta aacagtattt tttatggttt
     8461 aacctttgcc tgtgggtcaa tcattgtaaa ctctttggcg ggttttgcct ttgcgaaggt
     8521 taatttttca ggcaagaaaa ttatctttgg agctttgtta gctctgctga ttgtcccgat
     8581 ggagacggtt ttaattccgc aatttaccat cgttaattca ctaggcttag tgaatactcg
     8641 cttggcagtg attttaccag cgatggccag cgcgtttaat atctatctgt ttagaaattt
     8701 ctttatcgcg attccagaag aaatcattga atcagcgaaa ttagatggtg caaatattgt
     8761 taaaattttc tttcacatca tgctgccaat gtctaaacca gccatagcca cagtaggtgt
     8821 cttatccttt atctccagct ggaatgatta tatttggccg ttaatggtct taactgacag
     8881 cagcaaattt tcaatgcaag tggcaattac tacgatcaat actacacagc cagtttatat
     8941 caatcaagtg atggctgttt taaccattgc aacgattcca ctgattattg tttatatcgt
     9001 tgcacaaaaa tatattctgc agggacttgg cggttctgga acgggtataa aataggagtg
     9061 aatagtgaat gttgcaaaaa cttttaaagg ccaatgagtt tattgagaaa aatgctcaaa
     9121 cagtcaatca agaataccgg aataaatttc atttgatggc tccgattggc tggatgaatg
     9181 atccaaatgg ttttgtttat tatcaagagg agtatcactt gttttatcaa tattatcctt
     9241 acgacagcat ctggggacca atgcattggg gccatgctaa aagcaaggat ctaattcact
     9301 gggagcattt gccagtagca ctggcaccag gtgaaagcta tgacaaggaa ggctgttttt
     9361 ccggcagtgc catagaaaag gatggcaagc tgtatttgat ttatacagga cacaatgttg
     9421 tggaagggca agttcgacaa gttcagtgtt tggcagtttc agaagatggc attcactttg
     9481 aaaaatatgc cggaaatcca atcatcgcag aagatcactt agcttctgtt gcaacgaccg
     9541 aagatttccg cgatcccaaa gtgtttcagc gaaaagaaga ctattttact gtagtcgcta
     9601 ctaaaactgc agatgctcgt ggacaaattg tgatgttcca ttccaaagat ttactaactt
     9661 gggagtttta ttccgtattg ctggagggaa ctccagaaca aggcattgta tgggaatgcc
     9721 cagacttgtt tgaactagat ggcaaagaag tcttgattct ttctcctatt caaatgactc
     9781 cacaaggcaa tgcttaccaa aatacgaact caactgttgc tttcatcggt actgtcgatt
     9841 ggcaaacagg caaatttcta gtagagaatt ttcatgaaat cgatggcggt ttagattttt
     9901 atgcacccca gacctgtgaa aacgaagcag gccaaagaat aatgattgct tggatgcaaa
     9961 tgtggcagcg taaaatacct acgaatgatc tgggccatgg ttggtcaggt tccatgacct
    10021 tgcctagaga gctgagggtg gctgataatc aattgttgca acggccagtt tcatcaattt
    10081 atcaagtaat tgatccgcag gaaaggattg aatgggcgaa gactgaagag aatcgcttct
    10141 tactgaagga cttagtatat gaccagcact atttacgctt ggaaatcaat ttaaaacagg
    10201 caacgaaact agaacttcac tatgcgaagg gacccgacca tacattactg cttaactatg
    10261 atgttgaacg ggagtgcttt actgcttcca gggaacacat gccgtatgat ttatcaggag
    10321 ctgaaaagga agtgttgaaa aagcgcagtg tgacgattcc tttggaaaat aattgtctaa
    10381 ccatcgaaat tttccgtgat acttcagcta ttgagctttt tctaaatgat cgtctgacta
    10441 tgagtacaac tttttatgag acagaaaaag gacaggattt tcttttgctg gcagacagtg
    10501 aagtaacctt ttcattggaa agcggcttaa ttaatctata gaagggagag ctcagtgatg
    10561 atttatggtt caatcgaagc aggcggcaca aaatttgtgt gtgccattag cgatgaagaa
    10621 atgacgatca aggaaagagt gagctttccg acaacgacac ctgaggaaac aataccttta
    10681 gtcattgact tcttcaaaca ataccaagca gatttagcag ggattgggat tggctcattt
    10741 gggcctatcg atatccatcg cgattccgcc acttatgggt acattacctc gaccccgaaa
//
LOCUS       DAZKMB010000097        10718 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000097 DAZKMB010000000
VERSION     DAZKMB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.097"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..1461
                     /locus_tag="ACG8V3_002199"
     CDS             37..1461
                     /locus_tag="ACG8V3_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0300686.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1642..2415)
                     /locus_tag="ACG8V3_002200"
     CDS             complement(1642..2415)
                     /locus_tag="ACG8V3_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0300687.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2412..3317)
                     /locus_tag="ACG8V3_002201"
     CDS             complement(2412..3317)
                     /locus_tag="ACG8V3_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300688.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3471..3791)
                     /locus_tag="ACG8V3_002202"
     CDS             complement(3471..3791)
                     /locus_tag="ACG8V3_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0300689.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            4265..4951
                     /locus_tag="ACG8V3_002203"
     CDS             4265..4951
                     /locus_tag="ACG8V3_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0300690.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4953..5897
                     /locus_tag="ACG8V3_002204"
     CDS             4953..5897
                     /locus_tag="ACG8V3_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0300691.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            6096..6740
                     /gene="pcp"
                     /locus_tag="ACG8V3_002205"
     CDS             6096..6740
                     /gene="pcp"
                     /locus_tag="ACG8V3_002205"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0300692.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            6819..8096
                     /locus_tag="ACG8V3_002206"
     CDS             6819..8096
                     /locus_tag="ACG8V3_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300693.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            8354..9571
                     /locus_tag="ACG8V3_002207"
     CDS             8354..9571
                     /locus_tag="ACG8V3_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0300694.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(9892..10641)
                     /gene="pnuC"
                     /locus_tag="ACG8V3_002208"
     CDS             complement(9892..10641)
                     /gene="pnuC"
                     /locus_tag="ACG8V3_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0300695.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         3379 a         1977 c         2189 g         3173 t
ORIGIN      
        1 tttgataaaa gcggttttat tttggaggat gcttacatgg atacagagca acagaaaaaa
       61 ggaattggtt taattccact tgctgcctta gtaattggtt cagcgattgg aggtggcgta
      121 tttggtatta tgacggacat ctccggatcg gctggcggcc cagctatttt cagttgggta
      181 ctcgtaggaa cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa taaaaaacgt
      241 ccagatttaa aaggcgggat ctttagttac gcagaagaag gttttggccg tttcgctggt
      301 tttatcagcg gttggggtta ctggctcaca tgctggttag gcaacgtagc ttttgcaaca
      361 ttgttgatga gcgcattagg atatttcttc ccagtatttg aaggtgggca aaatctggca
      421 tcgatcgtga tgagtacagt ctttttatgg ggatatgcat ggctagtgaa tcggggagtc
      481 gaaaatgcta gtttcgtcaa tgctatcgtc acattctgta aattagtgcc attattttta
      541 tttattgtag tagctatcag cacgttttcg atccaacaat tcattgataa cttttctagc
      601 gacattatat tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt attgagttgc
      661 atcatggtga tgctttgggt atttgttggt attgaaggag cctctgtcgt aggttcacga
      721 gcaaaaaaac aatcagatgt tgggaaagca acgattctag ggatattcgg cttgattatt
      781 atttacgtct tggtttccat gcttccatat gggacaatga cgatgaatca gctgcaaacg
      841 atcaaggcgc agcctgctat gggctatgtg tttgaaatga tggttggtaa atggggagca
      901 gtcgtgatca acggcggttt gatcatttct ttagttggtg tttggctctc atggacgatc
      961 ttaccgattg aaacaatggg aaacatggct gaagacggac ttttgcctaa agcatgggga
     1021 aaactcaaca aaaatggggc accaacttat gctatcgtat tgacgacgtt atgtacgaat
     1081 gtctttttac tttcattatt attcactgac agtgcttata actttgctta cacacttggt
     1141 acagcagcta ttttcttcac gtggcttttc ttaggattgt accaaatgaa actttcttat
     1201 cagcggaaag aatgggtcca atttttagta gggcttttag caagtgcctt tcaagtatgg
     1261 gctatgctag tcgtagcctt taatgaagta atgcttgttc ttgttctttt cttgccaggc
     1321 atttattttt acctcaaagc acgtaaggag caaaacgagc ctgtacaaca tatccactta
     1381 gataagattt tacttatagg tgtgacgatg gtcggagtga ttgcaatggt tttatttgcc
     1441 actgggatgc ttcacgtata gaagtataaa agaagtataa agagaggttg tgagcctgtc
     1501 gtttattcaa aaacatacta ggaaaggagc tgtgacacaa atgttgtcac agctcctttt
     1561 tcagaataaa cggtgtttaa gagacagcca atcggcaata aggctgaacg tctctttcac
     1621 agcctctttt ttgatgagtc atcatacatt ttttcgctta aatacgagat atcctaatgc
     1681 taagaacaaa acgatgtaag ctaaattacc gaatagcatt tgattagtag aaagatgagt
     1741 caattgcttc atgatctctt cgtttccaat ttggttttgc agattcaaca tactgatcgg
     1801 attccatttg atccactccc atttttggat gagtgcaata aggataccag atatgattga
     1861 cgaagcaaaa taaaagacga ttcctgctga gatggatgcg ccggaactat tgataaagca
     1921 agcaatcatc aacactaaac ttaaaatcag ccataaaccg atataactac ccaaagtata
     1981 agtgaacaag ctttggatca gtgtttgacc agtagaaacc ttctccgtga aggaaatcga
     2041 agggaaaaga atcagtttca tcaacacagt gacaataata gctaaaaggt ataaataaac
     2101 agaataaata actaatgtga tccatttgct tactaatatt tctcctcgag aatactttcg
     2161 ataaaggaga tttttcaatg taccatattg tgcttccatc gaaatgatcg tgcttgcagc
     2221 tgcaatcatg ataaagacaa tccaagatgt ggcactaaaa agctgagcag tcatttcaac
     2281 tggatcaatg atcgtcgtgt attttttggc tagaagtgct gttcctatga gcaaaacaac
     2341 taatagaaca gataaaacag cagttgactt tttcttgatc aatttaaata attcttgttt
     2401 tacaagcaat gtcatttttc ttcttccccc tttactgaat ccagaatttc caatagagaa
     2461 gattccaaat cattcgtcag atgctggacg tctgtcacat ggatattttg tgcagacaat
     2521 agctggatca cggttcctaa taattcttcg ttttctttta aaagcttgat tttatcagga
     2581 tcttccaaag tatatccagc ttctgtcaag atcgttcgag cagctagatc atttgttgta
     2641 gagataacga tgaaatcttg cgctgtttgg ttcaattcag ccattgtaca ttgtttgatg
     2701 atctttcctt gattgatcag gatcagctca tcgaccattt tttcaagctc gctgagtaag
     2761 tgactggaga tcaagaaaga ggtgccctga gtagccaagt cggtaatgat ttttcgcaca
     2821 tctcttgttg cttgtggatc caaaccgttc atcggctcat ctaaaataac gatagacgga
     2881 tggttcagta aggcaagagc aattcccaat ttttgtttca ttcccaacga gtaggatttc
     2941 gcttttttgt gaatgtactt ttccatcttg agctgattca cgatagtgtc gatagcagat
     3001 acatcgttgg tttcagagaa taacttcaga tgatcatagc ctgttaagaa tggatagata
     3061 cccgggtatt cgatcaatgc tcctacttgt tccaaccctt tatgactaga gggagaaatc
     3121 tcattttcac caatacagat tgttccatct gagtaatgga tcagtcctaa aatagctttc
     3181 atgatcgttg tttttccagc gccattagga cctactaatc caacgatttt gccaggggat
     3241 aagctaaaac tgacatcgct gagggcttgt ttgtgaccaa agttcttgga taattcttgg
     3301 accgaaagac ttttcaaaaa tattcactcc ttttctatag atagtacaag catacacctg
     3361 atgagtggag aagtcgctag gtcattggac ttatttctta ttttttcata aaaaaattga
     3421 atattaaata aaaaggctta ttttttctga tcagtactta ttccaagtgt ctagtggccg
     3481 ctagtaatct tgatgccaat gatactgatg agtagcatcc ccacaaagaa ccaagtggaa
     3541 agattcagct tatcatgaaa caagaccaca ccaattatga ccgaaccaac tgcgccaatc
     3601 cctgtccaga taggataaga gagactcatc ggcaaacttt tgattgcctt tgacaaaaag
     3661 taaaaactag caatcatgcc aatcactgtg taaaaagaat aattgatttt agaaaaacca
     3721 tcactccatt tcatcatcgt tgaccagaat acttccaaaa ttcctgctat gattaattcc
     3781 atccatgcca tacttacatt cctccttaac tataaaaaaa tacacaaaaa gaggaaaatc
     3841 atcccttcaa agacctaatg gaatgatcat cctctttatc ctacccggtg gcagattgta
     3901 tatctttttc tgtagtatag cacacctatc cagatatcgg caatctcata ctttttagta
     3961 aagtgtacca agcactgacg gatatatcgt tagcggaaaa gaagatgaat gctttgcaat
     4021 ttcatagaaa gaagttgtta tttattaatc atttaaaaaa aacagtaact agaccttatt
     4081 tctatatatt atcgggcaat aaattaaaaa aagctttacc ttacaataag taagataatt
     4141 tttgtgataa aaaaataacg aaaaatattt aaaaatagtg tctatttcgt gagaaaacaa
     4201 ctatattttt tatgtgtttt gctttataat taaaaaaata ttgtcatttt atggaggaaa
     4261 atatatggaa aattatctta gccttttagg cgtcttgatt gttattctcg ggtttgcatt
     4321 taaattagac tcgatattga tcgtaatggt tgctttagtt gttacagctt tgactggcgg
     4381 tcttggcatt caaggaatgt tagaactttt aggtacaagt tttgtaaaca atcgcgggat
     4441 ggcgattttt atcatcatta tgttggcaac aggaacgctt gaaagaaatg gtctgaaaga
     4501 atcagcggct tccttgatca agcgattcaa gaaagtatct gctggtatga ttgtagatat
     4561 ttatgggatc tttcgaatga tctttgcggc ttttaacgtg agttttggtg gagttgctgg
     4621 atttgttcgt ccaatcattt taccgatggc attggggacc atcgaatcaa aagggttaga
     4681 agtccatcct aagcatgaag aagaactaaa aggtatggct tcagctatgg aaaatgtctg
     4741 ctggttcttt ggccaagtac tattcatcgg aggagcaggg gcgttgctag tccaatcaac
     4801 actgaaagat ctaggatatg acgtaacatt gggacagctt gctttagtgg aagttccagt
     4861 ggctatcacg gctttagttg tggcaagtgt ctacttctat cttaaagaaa agaaattagc
     4921 gaagaaatat tatccaggaa aggggcaata agatggactt ttttacaaat cctgatattt
     4981 tattaggcga taaattactg gagatccttt atatcgtgat gggactcgta ttgatttaca
     5041 ctggagtaag aaatttaaca gataaaacca atcctcatcg ttatgggtca gcctttttct
     5101 ggacggtctt aggggtagtt attgcaggag gaagatggct tccggcaatc gtgaacggtg
     5161 gcttgatctt tgcgatgacc ataccagcaa ttgctaagaa agtcagcaaa ggagaaagcc
     5221 ggcttccatc aaaagaatat atggaaaaaa tgtcagacaa actaggaatg aaaattttta
     5281 ttccagcttt gagtatcggt gtctttgcga tcttgtttgc tctatttacc aatttaggtg
     5341 ctttagtcgg cgtgggcgtc ggtgtttttg cagccatgct aatcttgatg ttcttttctc
     5401 gagataatcg accaagtaca tttttagatg atgcagcaga tatgctgggc acagtagggc
     5461 cgttaagcat gttgccaatg cttttagctt ctctaggtgc ggtatttaca agcgctggag
     5521 ttgggacagt gatttccaat gcagtaggta cgatcgttcc agaaggaaat gtgaacatcg
     5581 gcatcatcat ttacgctctt ggcatggtga tatttacggt aatcatgggg aatgcatttg
     5641 cggccatcac cgtgatgacg gttgggatcg gtgcaccatt cgtatttagt catggagcga
     5701 atccagcgtt aataggaatg gttgcattga cttgcggttt ttgtgggacc ctgctgacac
     5761 cgatggctgc taacttcaat atcgtgcctg tagcgatgct ggagatgaaa gacaaatacg
     5821 gagtgatcaa aaaccagttg tttattgcgt tatttatgct agttttccaa attgcttata
     5881 tgattctatt taaataatag aagagagggg ttgtgacaga ataattgtcg caacctcttc
     5941 taacattgta gtggaacact atttttttat aagaatatat taagtttgat ctgttatgtt
     6001 atttaaacta ttgtttcctt tatttacgct gttcaaggaa aatggtatga taaaagataa
     6061 ccatatgtta aatcaatcgg acggaggaac aacagatgaa agtactagta acaggttttg
     6121 atccatttgg tggggacaaa gtgaatccag cctatgaagc agtaaaaaaa atgccagatg
     6181 aaatcagcgg tgctgaaatc atcaaagtgg aagtaccgac cgtattcgaa aaaagcagtc
     6241 aagtagtcaa agaagccatc caacagcacc aaccagatgt cgtgatctgt gtcggacaag
     6301 caggtgggcg gtcagcagtc tcgttcgaac gtgtcgcaat caatctagca gaagcaagaa
     6361 tccctgataa tgaaggaaat cagccattcg atacggcttt agaagaaaac ggtccagccg
     6421 cttactttac ctcattgcca atcaaagcaa tgacaaaaaa tgtacaagat catggtctac
     6481 cagcgtacat ctcttataca gcaggaacat ttgtctgcaa tgatatcatg tatcgtttgc
     6541 ttcatttgat cgaaacacaa ttcccggcaa ttcgaggtgg gttcatccac gtgccattct
     6601 cacccgatca agtgatcgat cgtccagtgg gtacgccttc tatgtcttta gaagacattg
     6661 cttcttcttt gacttatgca gttgaagcag cagtagaaaa taaagaagat atttccggaa
     6721 atgccggaac aactcactaa tttagcaaga aacactggct ggttgctaac gttatgttag
     6781 tagccagctt tttgtaatta gaaaggaaag aaacagccgt ggaagaatgg caatccgtct
     6841 ttgaagaatg gtttccaaaa gaaatcagca aaagctatcc catcaaaatt tctaaacaat
     6901 acacaagcag tcaacgttgg gagatctatg caaaattaac gaaaaaacag cgggaactag
     6961 tcgataagca ccgccgatat ctaatcagct ctcgttttat ggaagaacat tatttagctg
     7021 caacagattg ggtgttttct gacttcaaga tcaatccgtt ttttcgaaca aaacgaagcc
     7081 agcaaaaatt gtattgtgag tgtggtagag aattaaaagt gcaatacatc gttaaatcgc
     7141 caaaaaccgg aaagatccta aaactaggca tcaaccactt tgccgatcac ctgcatgtct
     7201 ctccgacagt ggcagcttcg atccatcagg gaatgacaaa agttgatctg gccttagacg
     7261 aattattatg gcttaagcag aagaatatcg attttcctga aggactatgg caaaaatact
     7321 gttttgtctt gtaccaaaat cgtcggatga aacagccata tttaccggat ataaaattag
     7381 ctcagcggct cgcagaattt cgccaagtag aaatgccgat ttacatagcg gattatcaag
     7441 cattggaaaa tgaaatcaag aaaataagtg aacacatcaa tgggcagtca aaaaagcgac
     7501 agataaaaaa agaactattt gacgactttg cagaagaatt agtaaaggat gtagaagaat
     7561 tcttgatcaa ctatcgcgca tttttacgta aagattggca atccatcgta tatgaagaag
     7621 tgccagtaca tcctaatgct tattttgaaa catttatttc cgtgttgaga aagacaaaac
     7681 gtcaacgaac accagaagtc actgctcaga tggaatattt tgcgaaaaac caacgattca
     7741 tccaaccaaa aatctatcta tttatttgga agcaatattg tcgctatggt tttacagaag
     7801 ggttcttcga cagcattcct cggatagtca gaaatggttt tttaaaagta ttgcgtaaag
     7861 aacgagaagc tatacaatct gctgataaga aagaccgtac agtctcaaaa gaaaaatggc
     7921 aactagtagt gaaagatatc caatctggaa atgtgcaaga gacaatcgac aaatggaaag
     7981 gaaaacatta tcgttttact gaagctcaga aacaagcgtt ggaatattat cagaagttag
     8041 aagaatcact gcgtttcaat gatgaggcaa gaaagtatct gaaggaatta ctataaaaaa
     8101 catactaaga ttagagggga acatcattga cgaatgatgt tctcctctat tttctatttt
     8161 tagattatag tgaagagtga aagaaagttc gaacaaagcc ttgaggacta cgagtcccta
     8221 tcaaaaaccg aacttcttta ctgtcatttt agaatcaggt ttacgtatgt tggggccatg
     8281 agcccgtgta taggaaattg gaatcgaaat gaagcagtaa agttctttca aaacttaaaa
     8341 aggaggaaaa caaatgcctt acattcttgc attagatgtt tcaatgggac acagctacag
     8401 tgtcctatac aaagacgaca cctgcttatt tgaagacgag attgaacaca ctcaaagagg
     8461 atttcatgca ttactcgaag agatccagca cttgcctgag cgtccattga ttgtttttga
     8521 agcaacgggg atttattcca gaccagtcga aaagttcttt caagatcatc acttttctta
     8581 ctgtttgtta aacccgttag aggcgaaaaa acagatggaa gaaagcacct tacgcagttg
     8641 gaaaacagat aaatcggatg cccaccggct cgcacagact catcttaaaa atcaacgtca
     8701 gccaaaagaa gtacaaagca atttttactt agagatgcgc gatttagccc gtttttatca
     8761 agaaattgag aagaaaatca ctcgtctgcg catggattta cacaactgcc ttcaattgac
     8821 ttttccagag cttgaacagt tcttttcaaa caggctgacc ccttacgcat tgacattgat
     8881 ccgtcttttt ccccatccag attttgtatt ggcatctact cggacaaaaa taaaaaataa
     8941 actgatcaac gaaacacgga aaaaaatctc cgcgaatcgt gccgagcaaa aggcagatca
     9001 aatcatccac tatgcacagt gtgcctatcc agctgttgaa aaagacagta tccactgtca
     9061 aaaaacgatc tattacgccg aacttctcca agatttactc gaacaaaaaa aagcactggc
     9121 gacacaaatg atcaaaaaag ccgaaggctc tccttgcttt ttgttgtacc aaacgttccc
     9181 tgggattggc gcactcacag ccgctttatt gctcggtgaa ttaggagata tcacacgatt
     9241 taaaacccat aaacaattga atgcctttgt cggaatcgac atccgacggt atcattcagg
     9301 taagtatact ggccaagacc gcatcaataa gcgtggtaat cccaaagcac ggaaaattat
     9361 tttcttcacc attcgaaata tgattcgcca gcaacgcgca gctcctaatc atattgtcga
     9421 ttattactac aaattaaaaa agcaacctat ccctaagaaa gataaggttg ccacagttgc
     9481 ttgtatgaac aagcttctga aatgtatgca cgctatggtt agggcacata cagagtacga
     9541 ctatgcgtac gcggtctcag tggaccatta actaaaccac ttacagtata ccacttcttt
     9601 gcccagatcg aaaatttttt acgaacgggg ctttatttgg tgtgctttga ttttgtcgct
     9661 ttcgtttacg agctattttt cttgcacctt ttacttgact aatcgtagga aatagaggct
     9721 gggacagaag cgtttaactc caagaaataa gaagaaattc acgaaaattg cttttcaaat
     9781 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg attccgccgt ttatacgtgt
     9841 ttagagcgtg agacaaaagt gttctttact tttgtcccac gctcagtttt tttatgcgtg
     9901 ttgttgtgcg ttgtagtatt cttcttccat tttttgcata cgttcacgtt ctttttggtt
     9961 ataccaaggt gaaacggtaa atgcaaggac atcattcagc aagtaaacag agctgttcac
    10021 gaacatcgcc aaacttgcag atccatgcgt aaatgaaata taccaaagga ccatttgagc
    10081 aagccctgaa gctaaccacc agtaatattg gttgttgtaa cgcaagaaac tgatgatacc
    10141 agcagtcaag ctaatagaaa aagcaatggc atctacccac ggccgaggat catctgtata
    10201 acgaccaatc aggaatcctg aaacaagata gacaaggaaa gtactgatga ttgcgataaa
    10261 ccagcttttt ccagtgaatt ttcggatttt tgaagccata ttatgattcc attctttact
    10321 taacaatacg gggatatcca atgtgatcat ataagcaatc tgttcaccga tacttaaata
    10381 gttctttgct gaaaacccaa cgataataaa acaaatcgca gacaaaatac ctaaaatacc
    10441 gttgactgat tttgccgcat tgatggataa aatacacaat acaccaagcg tggtaccgat
    10501 aaatgtaata acggacagcc aagtgatggg actattgaca agagtcatga cctggcagcc
    10561 aagactgaaa aagaaaagat aataattttg ttgcggccag ccttttagct gatgagctaa
    10621 aaactgaaaa tatgacttca tgtatgagga ctccttaaga cactaaatgt agtgtgttct
    10681 gtatttacta acacaatcta ttgtatgctt ttcttgaa
//
LOCUS       DAZKMB010000098        10712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000098 DAZKMB010000000
VERSION     DAZKMB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.098"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..957)
                     /locus_tag="ACG8V3_002209"
                     /pseudo
     CDS             complement(1..957)
                     /locus_tag="ACG8V3_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            1448..2386
                     /locus_tag="ACG8V3_002210"
     CDS             1448..2386
                     /locus_tag="ACG8V3_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0300696.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2516..3163
                     /locus_tag="ACG8V3_002211"
     CDS             2516..3163
                     /locus_tag="ACG8V3_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0300697.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(3856..4794)
                     /locus_tag="ACG8V3_002212"
                     /pseudo
     CDS             complement(3856..4794)
                     /locus_tag="ACG8V3_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4794..5411)
                     /locus_tag="ACG8V3_002213"
     CDS             complement(4794..5411)
                     /locus_tag="ACG8V3_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0300698.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(5425..5595)
                     /locus_tag="ACG8V3_002214"
     CDS             complement(5425..5595)
                     /locus_tag="ACG8V3_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300699.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(5943..7433)
                     /locus_tag="ACG8V3_002215"
     CDS             complement(5943..7433)
                     /locus_tag="ACG8V3_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0300700.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(7476..7775)
                     /locus_tag="ACG8V3_002216"
     CDS             complement(7476..7775)
                     /locus_tag="ACG8V3_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300701.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8141..8425)
                     /locus_tag="ACG8V3_002217"
     CDS             complement(8141..8425)
                     /locus_tag="ACG8V3_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0300702.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(8944..9429)
                     /gene="vanZ-A"
                     /locus_tag="ACG8V3_002218"
     CDS             complement(8944..9429)
                     /gene="vanZ-A"
                     /locus_tag="ACG8V3_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0300703.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(9582..10493)
                     /locus_tag="ACG8V3_002219"
     CDS             complement(9582..10493)
                     /locus_tag="ACG8V3_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0300704.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3308 a         1864 c         1659 g         3881 t
ORIGIN      
        1 acttttttct gcttcttttt ctttctgttc ctggtaatca gaaaaattta gtttttctat
       61 atcgttaggt acagcttcca gtaaatgaac aatgaatttt ttgatattcg taataaagtt
      121 ctcttgattg ccttctcttt taccgatttt ttttaaatac gtttcccatt tagccgtcat
      181 ttcagcactc gttaaaaggt gctgactttc aactgcctgg cacaataatt ttcctttttc
      241 agttacaaca agcttattct taatcacttg gatatattct ttttgtttta acgcttcaat
      301 aatacttgct cttgtcgctt ctgttccaat cccctcaact tcttgtaaaa tcttgattgc
      361 ttcttcatca tcaaccgttt tattcgccgt tttcatagca gttaataatg taccctctgt
      421 aaaagctttt ggcggttgcg tttctttttc aacactctta acaccaactt cggcacgttc
      481 gccaatgata acgagtggta acgtttgaac gtcctctttt tcttctttgg tttgttgttt
      541 gaaaagaatt ttccaccctt cttgctttgg tgtctttccg atcgattgaa acagcaaatc
      601 agctactttc gtttgtatct tggtttcttc atacaagtaa tcaggtaaaa acatggcaac
      661 ggtcgtttta acgactaaag cataaatttt tcgttgtaaa tcgtccattt tcgctaatgc
      721 agattcggta ggtacttgtt ttgttgggat aatggcgtgg tgttcctgta ccttactacc
      781 gtccacataa cgctttctag gctctgtttg aaccatttca agatcaagtc ctaaaaaacc
      841 gctatatttg ccaaaattcg cttttaaata agcaaattcg ttctctgtaa taaatggtgt
      901 atctgttctt ggataactca ataattttgc ttcatacagt ccttgcatag cttttaaacg
      961 taaaattttg cgttctaaga cgttttaaat atatctgcgt atatttttat gtgttttatc
     1021 ttttcgtcgg caaatgcgtt aaaatttgcg aaataaaata gctcaagggc gaacgcagtg
     1081 agtgcattta cccttgatct atttttttga gtaaattagc ttttgaaacc gacggaaagg
     1141 aaacacaaaa cttttcagag gcaaggggtt tttaaggggc gcttttagcc ccttgaattt
     1201 atttgcttta agatatattt agttttaaaa tagttttaac gaaaaaaaga cataaaaaaa
     1261 gacgagcaac gctaccaacg ttctcgcccg gaataacaaa ccacacttgt tattcattcg
     1321 atgattagta tatcaatttt tgattttgaa tacaaaacaa aatggacgaa tggatacagg
     1381 cgaaatttat tttgcgattc cactcgtcct tttttcgttc caaaaattat tttaggagcg
     1441 ataaaaaatg aagaaaattg atgaaaaatt agctgcagaa atctcactaa aaaaaccggc
     1501 cgtctctaat agccggttaa gtaatagccg gttaagtggt caaactttgg gaaaatctca
     1561 acccgcatta agttttgatg ccatgacaat cgttggaaat ttgaacaaaa ctaatgctaa
     1621 aaagctatct gactttatga gtgtagagcc acaaatacga ctttgggata tacttcaaac
     1681 aaagtttaaa gctaaggcac ttcaagaaaa agtttatatc gaatatgaca aagtaaaagc
     1741 agatacttgg gatagacgta atatgcgtgt tgaatttaat cccaataaac tcacacatga
     1801 agaaatgatt tggttaaaac aaaatattat cgactacatg gaagatgacg gttttacaag
     1861 attagactta gcttttgatt ttgaagatga tttgagcgat tactatgcaa tgactgataa
     1921 agcagttaag aaaactgttt tttatggtcg taatggcaag ccagaaacaa aatattttgg
     1981 tgtccgtgat agtgatagat ttattagaat ttataataaa aaacaagaac gtaaagataa
     2041 cgcagatgtt gaagttgtgt ctgaacattt atggcgtgta gaagttgaat taaaaagaga
     2101 tatggttgat tactggaatg attgttttaa tgatttacac atcttgaaac ctgcgtgggc
     2161 tactttagaa aaaattaatg agcaagctat ggtttatact ttgttgcatg aagaaagtat
     2221 gtggggaaag ctaagtaaga atactaagac taaatttaaa aaattgatta gagaaatatc
     2281 tccaattgat ttaacggaat taatgaaatc gactttaaaa gcgaacgaaa aacaattgca
     2341 aaagcagatt gatttttggc aacgtgaatt taggttttgg aagtaaaata agttttattt
     2401 gataaaaatt gctaattcag tataattaat atttacgagg tgacataacg tatgaaaaaa
     2461 tcagaggatt attcctccta aatataaaaa tttaaaattt aggaggaagt tatatatgac
     2521 ttttaatatt attgaattag aaaattggga tagaaaagaa tattttgaac actattttaa
     2581 tcagcaaact acttatagca ttactaaaga aattgatatt actttgttta aagatatgat
     2641 aaaaaagaaa ggatatgaaa tttatccctc tttaatttat gcaattatgg aagttgtaaa
     2701 taaaaataaa gtgtttagaa caggaattaa tagtgagaat aaattaggtt attgggataa
     2761 gttaaatcct ttgtatacag tttttaataa gcaaactgaa aaatttacta acatttggac
     2821 tgaatctgat aaaaacttca tttcttttta taataattat aaaaatgact tgcttgaata
     2881 taaagataaa gaagaaatgt ttcctaaaaa accgatacct gaaaacacca taccgatttc
     2941 aatgattcct tggattgatt ttagttcatt taatttaaat attggtaaca atagcagctt
     3001 tttattgcct attattacga taggtaaatt ttatagtgag aataataaaa tttatatacc
     3061 agttgctctg caacttcatc attctgtatg tgatggttac catgcttcac tatttatgaa
     3121 tgaatttcaa gatataattc atagggtaga tgattggatt tagtttttag attttgaaag
     3181 tgaatttaat tttatacacg taagtgatca taaaatttat gaacgtataa caaccacatt
     3241 ttttggttgc ttgtggtttt gattttgaat ttggttttga acttatggac tgatttattc
     3301 agtccatttt ttgtgcttgc acaaaaacta gcctcgcaga gcacacgcat taatgactta
     3361 tgaaacgtag taaataagtc tagtgtgtta tactttactt ggaagatgca ccgaataaaa
     3421 aatattgaag aacaactagc aaaagatttt aaagagttat tttattttaa gtctttataa
     3481 catgagtgaa gcgaattttt aaatttcgat agaaattttt acatcaaaaa gccccctgtc
     3541 aaaattgacg aagggggttt tttggcgcac gcttttcgtt agaaatatac aagattgaaa
     3601 atcgtgtata agtgcgccct ttgttttgaa cttagcacgt tacatcaatt ttttaaaatg
     3661 atgtataagt gcgccctttt aaattttgag tgattatatt ttttgagtta gaaaaaggga
     3721 ttgggaaaat ttcccaaaat aatttaaaaa ataagcaaaa attttcgata gagaatgtgc
     3781 tattttttgt caaaggtgta taccttgact gtgcttgctg ttacattaag tttattttta
     3841 agttattaaa aaagaaatag cttttaaagt ttggctcgct gtcgctttat aaagctgatt
     3901 gacttttgat tgcaaactac ttaaagaaaa caaactcgga ctattcgttt tcttctcttt
     3961 ggtttgaaca tcagcaatta tcccctcttg attgcctatt ttagcttgtt tagaagaaac
     4021 aaaagctaaa agctcctctt gggttttaaa acgctgtgtg gggcttagaa cgcccttaaa
     4081 cgacccttgg tttactttta tactagcttc cacctcgaaa aaaggttctt ttttaaaatt
     4141 ctctatggct tcctggcgct gaaaaataag gtataaggtg ggcgtttgaa cacgtcctag
     4201 tgaaaatgta ccttgtacgc ccttctgttg taaatttaac gtatacaaag ggcttgcgtt
     4261 catgccgatc aaccaatcgg caatttggcg tgtttgcgct tcttgataaa agggatagta
     4321 attcattcca ggttgcaaat tttgaaaacc gcttcggatt acatcttttt ctaagctatt
     4381 gatccatagt cttttaaatg ttttatcttt tgaaaaggca tttgctttat ggataatcga
     4441 ccaggcgata ttttcacctt ctctgtcgct atctgttgca acaataattg tatttgcctt
     4501 tttgagaagt tctgcaacaa ttttaaactg ctttccctta tcttttgcaa cttcaaaatc
     4561 gtatcgatca ggaaaaatcg gcaaagattc aagtttccaa ttttgccact tttcgtcata
     4621 atgacctggt tctgctaatt ccactaaatg cccaaaacca aaggtgataa acgtttcatc
     4681 tgtaaatagt gggtctttga tctcaaaata accgtctttt ttggtgcttt gttttaaagc
     4741 acttgcgtag gctaatgcct ggcttggttt ttcagctaaa ataaccgtac tcattaacta
     4801 tccctctttt cattgttttt tctttgatcg actgtcacgt tatatcttgc tcgatacctt
     4861 ctaaacgttc ggcgattgat tccagtttgt tcttcaactt ctttatcgga taaaccattc
     4921 aaaaacaaat cgaaagcatg ctttaaacgt ggatcatttt ctttaaattt atgctgacga
     4981 cctttgaatt tgcctttttt cttagcaatt tcgattcctt gtgcctgacg ttccttaatt
     5041 ttttttcgtt ctgattctgc ttgatacttg tacaattcaa tgacaaggct attaatcaaa
     5101 cgccttaaat tttcatcttc aataccattc attgagggta aatttaagac ttccagggtt
     5161 gcccccttaa tttgaatttg attcatcaat tctgttaatt ctttattatt tcgtcctaat
     5221 cgatctaatt cagtaacaat aacaatatcc ccttcacgaa tatagttaag catagcttgt
     5281 aattgtgggc gttcgaccga ttgaccgctt aatttgtctg aaaagacctt agaaacgccc
     5341 tgtaacgctt gtaattgccg atctaagttc tgttctttgc tactgacacg tgcataacca
     5401 attttagcca ttttcaacca acctctaaaa ttctctcggt tgcaataacc aatcagcaat
     5461 atctactttt tcaatttcaa attgcttatc agaaattgtc ttttcgtaag cgataaaatc
     5521 ttgcgcatat tgttgctcat taaaaatagc caccacttcg tcattttcta aaactcgata
     5581 aataaatttt ttcattttac tcctcctatt atgcccaact taaatgacct attcaccaag
     5641 tcaattatac tgctaaaatc atattaggac aaataggtat actctaatga cctataaaag
     5701 atagaaaatt aaaaaatcaa gtgttcgctt cgctctcact gcccctcgac gttttagtag
     5761 cctttccctc acttcgttca gtccaagcca actaaaagtt ttcgggctac tctctccttc
     5821 tccccctaat aattaattaa aatcttactc tgtatatttc tgctaatcat tcgctaaaca
     5881 gcaaagaaaa aacaaacacg tatcatagat ataaatgtaa tggcatagtg cgggttttat
     5941 tttcagcctg tatcatagct aaacaaatcg agttgtgtgt ccgttttagg gcgttctgct
     6001 agcttgttta aagtctcttg aatgaatgta tgctctaagt caaaagaatt tgtcagcgcc
     6061 tttatatagc tttctttttc ttcttttttt actttaatga tcgatagcaa caatgattta
     6121 acactagcaa gttgaatgcc accatttctt cctggtttaa tcttaaagaa aatttcctga
     6181 ttcgccttca gtaccttcag caatttatct aatgtccgtt caggaatgcc tagcacttct
     6241 ctaatctctt ttttggtcgt cactaaataa ggcttgtata catcgctttt ttcgctaata
     6301 taagccatta aatcttcttt ccattctgac aaatgaacac gttgacgttc gcttcttttt
     6361 ttcttgaatt taaaccaccc ttgacggaca aataaatctt tactggttaa atcacttgat
     6421 acccaagctt tgcaaagaat ggtaatgtat tccctattag ccccttgata gttttctgaa
     6481 taggcacttc taacaatttt gattacttct ttttcttcta agggttgatc taatcgatta
     6541 ttaaactcaa acatattata ttcgcacgtt tcgattgaat agcctgaact aaagtaggct
     6601 aaagagaggg taaacatgac gttattacgc cctattaaac ccttttctcc tgaaaatttc
     6661 gtttcgtgca ataagagatt aaaccagggt tcatctactt gttttttgcc ttctgtaccg
     6721 cttaaaaccg ttagacttga acgagtaaag cccttattat ctgtttgttt gaaagaccaa
     6781 tcttgccatt ctttgaaaga ataacggtaa ttaggatcaa aaaattctac attgtccgtt
     6841 cttggtatgc gagcaatacc aaaatgatta cacgttagat caactggcaa agactttcca
     6901 aaatattctc ggatattttg cgaaattatt ttggctgctt tgacagattt aaattctgat
     6961 tttgaagtca catagactgg cgtttctaaa acaaaatatg cttgataacc tttatcagat
     7021 ttgataatca tagtaggcat aaaacctaaa tcaatagcgg ttgttaaaat atcgcttgct
     7081 gaaatagttt cttttgccgt gtgaatatca aaatcaataa agaaggtatt gatttgtctt
     7141 aaattgtttt cagaatgtcc tttcgtgtat gaacggtttt cgtctgcata cgttccataa
     7201 cgataaacgt tgggtgtcca atgtgtaaat gtatcttgat tttcttgaat cgcttcctcg
     7261 gaagtcagaa caacaccacg accgccaatc atgcttgatt ttgagcgata cgcaaaaata
     7321 gcccctttgc ttttacctgg cttggtagtg attgagcgaa ttttactatt tttaaatttg
     7381 tactttaaca agccgtcatg aagcacagtt tctacaacaa aagggatatt cattcagctg
     7441 ttctcctttc ctataaatcc tataaaatag gttgtttaat taacttggtt tgctttttca
     7501 ttcaactgtt tcaatattgc atgttttgaa aaagattttt ttcctttata agtcaatttt
     7561 tttccactaa tcgaataaat tattttgtta ttttctatta acttatatat ataatcttcc
     7621 ccctccgaag aaaaatactt atctgatttt gtttctaagt agatatttct cttttctaac
     7681 tctttcttaa acgtttctag tgtatagata tttgctaatt ttcttatctc caataaacta
     7741 ttttttatat aagttttaca ttcatcatga ttcatacaaa ctccaccttc tataaatgaa
     7801 tacaaaaaaa gcaatcaaac gatttccgat tgattgctta acaattctta aattcagtag
     7861 cttagatact tgaaaactct ctgatttccc tatataatga tagtacggtt atataccgtc
     7921 ttcaaacaaa gttaattaaa taacttctta cgagggaaga gttcatctga ctaactgata
     7981 agcgttggtt tggcaatctt atcgggctat gcatttataa aatgtcgtca aacattttat
     8041 aaatgtgtca tggctctttt ttcgtttcta ttcagttcgt tgtttcgtta tatctagtat
     8101 accgctttta aaaaaaataa gcaacgattt cgtgcattat tcacacgaag tcattgcttt
     8161 tttcttcttc catttctaaa tccaatgtta cttgttctga ttctgtttct ggttctggtt
     8221 ctgttggctc atttgggatt aaatccacta ctagcgttga gttagttaac tttgcaattt
     8281 gttctagtgt ttttatggtt ggatctgatt ttcctgattc tattcgtgaa taatttgatc
     8341 tactcatttc taattcttgg gagaattttt cttttgattt ggtacctctc atctttttta
     8401 agctttctct aaatgctagt tccaagtttc tcccccctct ttttgtgtaa taaaattcac
     8461 gctttattta aattatagct tttaatatct taataaacaa gtgattaatc acttgtgatt
     8521 tgtgattatt ccaaaagtag gtaatttcac caaccttttt caaaagtttt tatcgacgat
     8581 tattggactg gatcgtgttt cctaaagcga ttctaagcgc atgtttaatt ttaaagtggg
     8641 taattctctt taaaacgtcc tacaaagatt caaaaaaagc atggctgcta gtgggggtac
     8701 cgtcaggaaa atgcggattt acaacgttaa ggaaatttat taaagagacc aattcctcag
     8761 cactaaaatt gataattggg ctttctttgt tactatagtg ttaggtaagc tttttacttt
     8821 atcaccggtt ttcccctcac ttcacaccta cgggcgagtt tccccacatc ccgttttcca
     8881 tctaatccaa ttcattcaat atctttatgt gtatgaaaga tcagattgct tgatttagac
     8941 atcttacacg taatttattc ttaaatgggt acggtaaacg agcaataata cgagcaaaag
     9001 tatacctaca aaaataataa ctctgtctaa ttttttttga ttcatatgct tattgcttaa
     9061 accatataat ttcagtccaa gaaagcctcc aacagtattt gtaattacat ctgttatgtc
     9121 tgtcgctcca atagcgaaga taaattgaat tatttcaaaa gtaagactta aaaccagtac
     9181 aaaagcaaac ttaggtaaaa atccgatttc tttaaaattg acattcaaaa gcaagccaaa
     9241 tggaataaag attataacat tatctatcat ctctctgaaa ttcccagtag cagtaaatgg
     9301 agtcaagtta agacttcttt gatgataatt aaatactgat aaaatattgt attgtaattt
     9361 gaataacact aaccagatta gtgtcactaa atataaagct agcaatcctc tagataatat
     9421 ttttcccaat ataatttcct ctaattcttt tgtttaagtt gtatcattag tatagcaatg
     9481 tcctagttaa cgtgggtttt aaattaaaaa gctaacgtta ccccaaaagt gctagtacaa
     9541 taatgctgtt cattaatttc gttggtttca ttccgccatc cttacctcct tgaattagta
     9601 tgtgttgatc cgggaaacac tgtcacaatt acaccgtcta tattgtttcc tgatatctca
     9661 taacgaagat tagtcggcac atgaatggtg gtatttttag taacaggata ataaaagatc
     9721 tcatattttt ccccatttac actaacagaa atggtttttt cttcttttag gtaatccata
     9781 tattcctcga gaacgaaatt cttttctttc ataatcgcac tatgtggtaa accaacatag
     9841 cgaatatgcc atggttcata ttgaattcct gttaactctg ttttgtcctc tggataacgt
     9901 aaaatgaacc cgtatttcca agcattttct tctatccact ttccttcagg ggctcgttcc
     9961 attttcgtca agcttgatcc tacatctagt gataaacctg aattatgctc actataacct
    10021 gctggtaagg catactcagc ccccatttct tggtaaagca cactttgctc atcaaagtct
    10081 cgatagccac tattaataat aaaatgacta acgccaccct ttacagcatc attgaccatc
    10141 tctgaaaatt tttgtgctat ttcttttgac atataaatat tactatcaag caacccgtat
    10201 ccatttatta attcgtcatg tttagataaa ttcacgatat ctgacttcac actttcttgg
    10261 cgaacaggat atttactatt gattaatagc agatttcctt gataaacctg ttcttctgta
    10321 attgttttct cttgggtatt ttcagaagtc ccactatttt ctaaatgttc tttgggattt
    10381 tgatcataat tttgaaattc gactttttcc tgagaaaaca gtgcttcatt aacgtagtca
    10441 taacctaagt atattaagaa taacaataac aataaaaaaa acaacttctt cattttcagt
    10501 ctcctcctta actatatagt tggaagtatg aaatatttct agaatcttcc ccttaaacgg
    10561 cacaatctcg tttgagattg tgccgttttg ccccgtaaat tacagtcaat tatttcgccc
    10621 tactggattt actattatca ccaatgtaga tgaattttca taagtgatac ccatgctgaa
    10681 tttaacgagg ctgaactgcc tgttgacaat gg
//
LOCUS       DAZKMB010000099        10656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000099 DAZKMB010000000
VERSION     DAZKMB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.099"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACG8V3_002220"
     CDS             34..657
                     /locus_tag="ACG8V3_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300705.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8V3_002221"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8V3_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0300706.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACG8V3_002222"
     CDS             2153..3034
                     /locus_tag="ACG8V3_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0300707.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACG8V3_002223"
     CDS             3037..3807
                     /locus_tag="ACG8V3_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300708.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8V3_002224"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8V3_002224"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0300709.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACG8V3_002225"
     CDS             5118..6281
                     /locus_tag="ACG8V3_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0300710.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACG8V3_002226"
     CDS             6398..6808
                     /locus_tag="ACG8V3_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0300711.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACG8V3_002227"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACG8V3_002227"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0300712.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACG8V3_002228"
     CDS             9381..9665
                     /locus_tag="ACG8V3_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300713.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="ACG8V3_002229"
     CDS             9825..10061
                     /locus_tag="ACG8V3_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0300714.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="ACG8V3_002230"
     CDS             10058..10456
                     /locus_tag="ACG8V3_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0300715.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3723 a         1773 c         2253 g         2907 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
    10621 gaaatagtgg tttagaacta gtgggctgat ttttta
//
LOCUS       DAZKMB010000100        10576 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000100 DAZKMB010000000
VERSION     DAZKMB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.100"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            173..1117
                     /locus_tag="ACG8V3_002231"
     CDS             173..1117
                     /locus_tag="ACG8V3_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0300716.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            1131..2204
                     /locus_tag="ACG8V3_002232"
     CDS             1131..2204
                     /locus_tag="ACG8V3_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0300717.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            2194..4365
                     /locus_tag="ACG8V3_002233"
     CDS             2194..4365
                     /locus_tag="ACG8V3_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFQ0300718.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(4477..6648)
                     /gene="copB"
                     /locus_tag="ACG8V3_002234"
     CDS             complement(4477..6648)
                     /gene="copB"
                     /locus_tag="ACG8V3_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0300719.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(6668..8854)
                     /locus_tag="ACG8V3_002235"
     CDS             complement(6668..8854)
                     /locus_tag="ACG8V3_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0300720.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(8854..9063)
                     /gene="copZ"
                     /locus_tag="ACG8V3_002236"
     CDS             complement(8854..9063)
                     /gene="copZ"
                     /locus_tag="ACG8V3_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0300721.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(9076..9516)
                     /locus_tag="ACG8V3_002237"
     CDS             complement(9076..9516)
                     /locus_tag="ACG8V3_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0300722.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(9590..10088)
                     /locus_tag="ACG8V3_002238"
                     /pseudo
     CDS             complement(9590..10088)
                     /locus_tag="ACG8V3_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            10290..>10576
                     /locus_tag="ACG8V3_002239"
     CDS             10290..>10576
                     /locus_tag="ACG8V3_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFQ0300723.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT         3009 a         2224 c         1896 g         3447 t
ORIGIN      
        1 atttaaatac gcaaattttc ctcattattt ttaattttac tcaatttaat cgtattctca
       61 tttcaaaacg aatgtacttt aaaaaaacat aataaaatca ctttgcttgt aggatgtatt
      121 cccttccatt acaatgaaat aaatagcaat tttatgagaa ggtgatgtga aaatggaaga
      181 aaaaatcgaa gaatccaaac aaaaaatcga tcagttgata caagaattag aaaaagcggt
      241 catcggcaaa cgttcaacta tccaattgat gatcgtggcc ctattagcag gtggtcatgt
      301 cttattcgaa gatattcccg gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc
      361 tgtacaggga accttcagca gagtccaatg tactcctgat ctgctgccta gtgatattct
      421 tggtttttcc gtatacaatt cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt
      481 taccacgatc ttgttggcag atgagatcaa tcgcacgact cctcgtactc agtcagcact
      541 tttagaagca atggccgaga atcatgcaac gatcgacaac aatacatacc ctctagacaa
      601 tcattttttt gtcttagcta cgcaaaatcc aatcgagtat gaaggaacat atcctttgcc
      661 ggaagcacag ctcgatcgtt ttttattccg actacaaatc ggttatcctt cttttgatga
      721 cgaacttttg ctacttttag ataaattcga aaaagtgttg gataacttaa atgaagtatt
      781 gaacagttat gaaatagaag aaataaaaac aaatgtggcg aatgtctttg tcagtcccga
      841 agtggcatct tatgccttgc agttagtgtc agcaacaaga agccatgctg ccatccagct
      901 tggcatcagt ccacgaggaa gcctttcctt tattcaggca gcaaaagctt atgctttaat
      961 ccatgggaga aattacgtca cgcccaaaga tttgcaggat ttagttcctt acgtgttttc
     1021 ccatcgcctg attttttacg atcgttcatt gaaggaagac gaaaaaaaac aaatcattca
     1081 tactcttgtt gcacaaattc ctgttcctgt gaggtagatt gaggtagata atggaaaaaa
     1141 tcaaagatta tttcattaat tttgtgttat ttctggtata tttcatcatt gtgctttttg
     1201 ctcttgtatt caacaattct ttaggctggt tcttgttggc cttttttact ctttatgtat
     1261 catggagctt gatttcatta agcagctttc taaagaaact ttcggttact tgtacattgc
     1321 agccactcgt ttatcgcaaa aaaagtatgc aggttatttt ttccatagca aaaaagagaa
     1381 ctttttcttt acctctccct cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa
     1441 aacaagagat tttgattctt acaaataaac cgcaagaagt ccaattgctt tggcagccaa
     1501 aagaaagagg atgccatgaa acattaactg tcatttttac agcctatgat agtctaggac
     1561 ttttcagaaa aagtgcaaaa agagagatct cttttcctgt gactgttctg cctgcttttt
     1621 gtaagattca agcagaaaaa ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt
     1681 accaaagagg tcttgatttt cgggaacatc gcgcttatcg tccaggtgat tctttcaacc
     1741 gaatcgattg gaaattgtct gctcatactc aagagtggtt ataccgtgaa tacgattatc
     1801 aagaagaaga atgttctcct cttttttgct tttggggttc gcacagtcca aaatttgaac
     1861 ctctgctaga cttgtatttt tctttatggc atttgagaaa agaaaagcag ccggaacttc
     1921 tgatattagg caaccgcgcc tttcatggca aagaccccgg tcatacctat tttgcttctt
     1981 tggaagctgg cgatgagaaa gcttttttcg cacacctaaa aaaatatgca aaaaaacaaa
     2041 tcgtcctctt tgttcctgag aattctcctg aagtctcaga agctattgaa acattgtcaa
     2101 aagatcgaac ggtttatctt gtttatttcg tagaaaaaca actcatggtc caagaaaaag
     2161 acaagatatg ttcattgcca ggaggtgaat ggattgacga ttaaatggga aaaatggctg
     2221 tgtggcgttt actcctttgt gatactaagt gtaggtgctc cgatatttgt agtagctaat
     2281 caattgcaaa acagctacct tcttatccta actattggtg tcctatgcct tattgtctgc
     2341 tgtattccta aaaaatgggt acgtttgctg ctttctttgc cagtaatgat aggttgtttg
     2401 tttttgtatt ttccttcgaa gacattttcc ttattatgga tcttacagtt tttttcgtca
     2461 gtcacagagg aattcttcca tctatcccaa ggtaaacttt attatttgcc agaaaagaca
     2521 gctttttttc tcataatgtt gctgatctat ttatttatta cattgaccgt ggattacgac
     2581 tatttgtacg caccctttct ttctttgatg gcttacttca tgatgcttac cgtttttcgg
     2641 gaaaaagatt tactgtacga aatgattgcg gtagtaactg ttttgttcgt gtatctggca
     2701 gcaaggcaat ttttctccat gcggttgttg tcgggaaaca ctcgattcca gtcattgact
     2761 acagttctgc ttatttgttt cttaactatt agcgctatct tgcctttata tcttccgaaa
     2821 gtacaccaat ctctcgagga aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg
     2881 ctttatgata cactccatga gtatcaatta accggaagtg caagaacagg cttcagtgag
     2941 aatgatgaac agcttggtgg acctttatat gataatggcg aagttgtttt tacagcgaac
     3001 caaaaaggaa atggctattg gaagatcgaa aataaaaatc attacaccgg ttctggatgg
     3061 atagaagatc aggacgcacc catccaacca atccaagaat tccctgttga catcacgagc
     3121 taccaaaccg ttatggaaga caaagaaaac attacattga ccctaaaaaa acaagctttc
     3181 ttaccttatc cttatggaaa tactaccttg actgattttt cattagctag tggacaaacg
     3241 ctttactatt tgcctgaaag ccagcgattc attcctgtcc cgaatgaatc gacacgttct
     3301 gcaactttac aaacagaaag gttagttttt acgagagaag aattaaaaag cagtcagtta
     3361 aaagctgatg atttagaaaa tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa
     3421 cgtattgagg acttggcggt cgaattgacc aaaaatgaag caactttaat tcgtaaagtc
     3481 gaagctgtcg aacagtattt aaaaacaagc ggaaatttcc gctattccaa aacggatacc
     3541 ggacatactc cgaaagaaca cgattatgtg gattatttcc tctttgattc aaaagtcggc
     3601 tattgtgaca acttttcaac ttctatggtc gtccttcttc gaacacttgg tatcccagct
     3661 cgctggacaa aaggttttac aaatgggctc agaacaggca cagataatca agggaatgag
     3721 gaatacagca ttctcaatag tcatgctcat tcttggccag aggtatactt tcctggattt
     3781 ggctggcttc catttgaacc aaccccttca tttgccaatc caatgggttc acaaccagtt
     3841 gctgaaacaa ccgaaaccgt ccagcctgct atcgaacaac caaatgaaag cgaaaatatg
     3901 gcagaaacta tttccagtga gagatctgag gtcattccat cagaagaaac aacactcaca
     3961 ccagcttata aaatcgtctt attcagcctt ggggtgtgtt tatacgtctt gttgttcttt
     4021 ctattgttat tcagaaaatc tctgcgctgg acaagttatc ttatctatct tcattttttt
     4081 gcttctacta atacgattgc atatcaaaaa cttcttaacc atttggaaac attggtaccg
     4141 cgcaaacccg cagaatcttt gcaggaatat agcctgcgaa tagaacaaga attcaagcta
     4201 gatctgcgtt tcattcgatt aactactcaa tatgaagcag aaatatatgg acgacaagca
     4261 gatccggtaa atttcgataa aaaaacctat ttagaaatta cgcatataat aagaaaaacg
     4321 gtatacaaaa aagttctgac aaaagggagc cgcttcaaaa aataagataa aatttctgga
     4381 gaatctactt ctggaatgcc gagaaccagc tgcttgagta tcgattattc gaaaaaaagg
     4441 ttgtgacacg ttttttgtca caaccttttt tcattattat ttgattttca atgtcaaagc
     4501 atttagagct acaactaccg tacttaacga catcaagaca gcaccaactg ctggacttaa
     4561 tatgatacca attggcgcaa gaattccggc tgctagcgga attgcaagaa tattgtaacc
     4621 tgccccccac cagaggttct ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa
     4681 atgcaaaata tcttttggat cgctgtttgt caatacgaca tccgcagaat cgattgcgat
     4741 atctgtccct gcaccgattg ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt
     4801 gatcccatca cccaccatga ttactttttt cccttggtca aggtatttct ggacaacagc
     4861 ttctttatca tctggtaata aaccgccata atattcttcg atccctaaat aatctgctac
     4921 tgcttgggca gcgtttttat tgtcaccagt aagcatgaca ggagtgattt ttctttcttt
     4981 caatgttcgg ataaattctt ttgcttctgt ttttactacg tcacctaaag caacgattgc
     5041 agctagatga ttttctaaaa tcaggaagct gatggtattt ccttgttctt ggtaaggttt
     5101 caataatgct tgttctacgt ttaatccgag acgctcaact tctttttcat taactatttt
     5161 cacttgttgg tttttgactg ttgcctctag cccaactcct gaaagatttt ttagattttc
     5221 ggcttgataa ggcttgattt catgttcttt caaataattc ataataccaa cagccaatgg
     5281 atgattggca ttcgcttcca atgctcctaa atattgaagg atttcatttt tattgaacgc
     5341 atcatcaagt acttcgacac ccgttacagt aaattttcct tctgttagtg ttcctgtttt
     5401 gtccagcatg atcacatcta gatcattggc ttgctccagt gcattgcggt ttttcaaaag
     5461 taatccgttt ttagctgcta ttgatgtaga gcgagcaaca actaatggga tagctaaacc
     5521 tagagcatga ggacaggcga tgataaagac tgttaccata cgttctaatg cattaggaag
     5581 atctgtcaag aacaaccagg cgatgaatgc caggataccc acaattaatg cgacataaaa
     5641 cagccatttt gccactttgt cagataatga ctctagcttg gatttttctc cttgtgcttt
     5701 tcgcaccatt tccataacct tcgctagata accattttca ccagttccag ttacttcgat
     5761 ttcgatcgtt ccatccccat tgatagaacc gccaatcact ttgtcattgg cttgtttttt
     5821 gattcctctt gattcgccag tcacagctga ttcgtcaaca attgtttcac ctttcaagat
     5881 tacaccatct gttggcattt tatcaccaga ccggacgatc aaacgatcac cttcgtttac
     5941 ttctttcaat gaaactgtct cttcttttcc atctttcgtc aagcgtttta ctgattctgg
     6001 cagtaattct gctagttttt gtaaagcatt gctggcattc gacacagcat tcatttcaat
     6061 ccagtgacct agtagcataa taacgatcaa agtagccaat tcccagaaga aatccatgac
     6121 gtgtgtatgt ggattgagca gatttgcgat aaacgaatag acactataaa tatatgcaac
     6181 tgtaatcccc atcgcgatca gtgtcatcat agcaggactt ttttgtttca attccatctt
     6241 agctccactt aaaaatggct gaccgccata gataaatagt atagttgcta agatcaatac
     6301 gagccaatca gaaccaggaa acgttacttg aaatggaaat tccattccca tcattggtga
     6361 aaaaagaatg attgggatag ctaggataag cgatagccaa aatttttgtt tgaaatttcc
     6421 catgtgcatg gaatgatcca tgccgccatg atcgtgatcc atgtgcatcg aatggtccat
     6481 ttgtttgctg tttgctatat ctccatggtt atgtcccatg tgcatggaat ggtccatctg
     6541 gctatggtca tgtttcatct ccatctgttt ttcatgagat tctgtacttt ctttgtttac
     6601 tacttctttt ttatgttcat gttttttctc catatcgttc ccattcattt ttactccatc
     6661 ccttctctta ctttttcatg tgccgattta agctcaggga gttcaacaat acactgacgg
     6721 aactaaacgc catcgctcct cctgcaatga ttggattcaa aaagcctagt gcggcaaaag
     6781 gaataccaat cgtattatag acaaaagccc agaaaagatt ttgtttgatt tttctcaatg
     6841 tcaccctaga caactcgatt gtttgtgcaa tggatgccaa actgctactc atcagcgtca
     6901 catcggctgt ttccatcgcg atatccgttc cacttcccat tgccatacca acatcagcta
     6961 atgctaaagc cggtgcatca ttgattccat cgcctgccat accgactttt ttgccatctt
     7021 tctgaagttt ttctacgtag gctgctttat cctctggaag tacttcagca aagatatgct
     7081 ttggatcaat accaacttgt ttaccaatcg tttcggctgc cagtttgttg tctcctgtca
     7141 gcataaaaac atctaatcct ttattctgga gctgcttgat tgcttgtttg gcttctagtt
     7201 tgatttgatc agccacggca atcaacccga tcacttgctt ttcatcagct aaaaacatga
     7261 ctgtttttcc ctgctgttct aattctaacg cgtgttcttg gtattcatca aatgataggt
     7321 tcaattcaat taatcgttta cgtgtacccg caaaatagcg tactccgtta atcgtaccgc
     7381 tgattccagc acctgggtga gctgtaaaat caatcatatc gtatgttgct gcttcttgca
     7441 gttttccgta ttccacgatt gctttaccta gtggatgttc agaagcttgt tctaaagtat
     7501 aaaaaataga taggacttct ttagaaccga ctagatctgt aactttgggc tttccttctg
     7561 taatcgtccc agttttatcc aaaacaatgc tgtctaagtg agcagctgct tctaatgctt
     7621 ctccgccttt aattaaaatt ccatttcgtg caccaacacc cgttcctacc ataatagctg
     7681 ttggagtcgc taaacctaat gcacatggac acgcaatgac taatacagag acactatgga
     7741 tcaatgcgag ttgccaatca ccagtgatca aacctgtagc tatgagcgtg ataaacgcga
     7801 tgactaatac gatcggaaca aaaatccctg agatccgatc tgcgattttt tggattgggg
     7861 ctttgctccc ttgagcatct tccaccattt gaatgatttg cgctaatact gtctgcttgc
     7921 cgacttgtgt gacttccatg tgtagcagac cattcgtatt gattgtcccg ccaaaaagcg
     7981 tgtcatccgg attcttttct acaggaagat tttcaccagt aagcatactt tcatctatcg
     8041 cactgcttcc tgaaataatt cgaccatctg ctggaatctg ctcaccagga cggatgacta
     8101 gttgatcacc aacgactact tcttcaatcg ctaacgtttc ttctttcccg tttcttatca
     8161 cttgggcggt ttttgtttga agagacatca attgtttgat tgcattacct gttttcgttt
     8221 ttgctgtatg ttccaaatat ttgccgagca aaatcagcgt gatgatcata ctgctgcttt
     8281 caaaataaag atcttggggg tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg
     8341 cagccgatgt tccaattgct accaatacat ccatatttgg tgctttcgac ttgattgcat
     8401 ggtacgcacc tttataaaaa cgagcaccga cataaaattg gacaggagca gacagcacta
     8461 actggacgat tggaaaatga aaaaagtgaa caatcgccgc atggcttcct aacatcatag
     8521 caatcattga caacatcaac ggcaaagtca aaaccgtact caatataaga tcgaatagca
     8581 tttttttcaa atacgcttgc ttttcttctg caattttttg tttgtgggct tcatcgtaca
     8641 aaattgcacc atagccgatg ttttctacgc tttggattaa tttttcagtg gtcgtccctt
     8701 catatttgac acttgctttt tcagtggcca agttgactgt ggcattgacc acaccaggtt
     8761 gtccgttcag ctctttttcg atcctagcag aacaatttgc acaagtcata cctgtaatga
     8821 cgaacgtatc cattttggtt tctgctgttg ccatcaaatc acctctgctt tataacccaa
     8881 tccattgatt gcttgacaaa tcttttcgga ttgaactttt gtttcatcaa acttgaccgt
     8941 accgtttgct tttttcaaat ttactttcac tttttgcacg ccgtcaagtg cgcttactgt
     9001 ttcttctact cttgctgcac aatgattgca gctcatacct tcgattgaaa attgttgttt
     9061 cattttttct tcctcctatt gatgttgttt acattcacat tgacctggga tacagttaca
     9121 ttcgatcgta tctactggtt gcttttgttc cagttgtttc aaaatctcat tgatatcttc
     9181 ttgagtcaaa gtggcttctt ctactagatc cgcaatcgtc tctccaatcc tttttgcaca
     9241 gatatgggaa aaaagatttt ccgtcgcact gcgaactgtt tccatctccg agacagctgg
     9301 atgataaatg aatttttttc cttcttgttc tgtccagatc acttcttttt ttaccaaacg
     9361 acccagcaaa gttttgatcg ttgctggttt ccaatccatc gaatcagcta agatctgcgt
     9421 gatttcttgg gctgtcgtct gtccaagtgt ccaaataaca cgcatgatct cccactctga
     9481 atcactaatc tttactggcg ttttgacttt ttccatttat ttcacacctt tcgtttacaa
     9541 atgtaatcat taacttatga atacagatta cacttgtaaa caaaaactgt caattatttt
     9601 tgctctctat tttcaattta gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga
     9661 tcagtttatt tcttgcatta gctacttgga tcagctgctg tatttttggt tcattgtttc
     9721 cttcaaaatg ccagataaaa gataaaaatt ctaagtattt cctgtttagt ttctccgaaa
     9781 atgattcagg catatctggc cgagattttt tgtttatctt tctttttaga gaacgccaaa
     9841 ctactcggta gatggcgaga taacgcggca ctttaagcca aatgatctta tctgcttctt
     9901 taatcctttc ctctaacgta gaagtataat ttccttcaac gatccatgac ggattagata
     9961 ccataaacga ttgctgttct tgcaaaaacc acttttttgc ctgaatgcgg aaagccggtg
    10021 atttctttga tcttccttgc gagggacgat tttcccgacc cactttgtcc aatcacgatg
    10081 atcttcattc ttctccctca cttctctttg gaatttggtt cgatcatacc acttataacg
    10141 ccgcaaaaaa acaggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    10201 ccatttttat tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcacgacaa
    10261 aataaacaac tgagaggacg atatgctata tgaatgattc tatcaaaaaa atgctgcgat
    10321 taatagagaa agatttgatg attacagagg tctcttacga gacctttcag aagaaaaaga
    10381 cgttgatcgt cgatgctgtt ttctcacctg ctccccatac ctgtagaaat tgtggttcta
    10441 ctgtggtaga tggaaacggg aaagtaattg tagtgaaaaa tgggaagaag gaaacgattg
    10501 tccgctttga acaatacaat cacatgcctt tggttatgcg cttaaaaaag cagcgctata
    10561 cctgtaaaaa ctgtcg
//
LOCUS       DAZKMB010000101        10561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000101 DAZKMB010000000
VERSION     DAZKMB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.101"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            195..1091
                     /locus_tag="ACG8V3_002240"
     CDS             195..1091
                     /locus_tag="ACG8V3_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0300724.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1241..2182)
                     /locus_tag="ACG8V3_002241"
     CDS             complement(1241..2182)
                     /locus_tag="ACG8V3_002241"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0300725.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2308..3429
                     /locus_tag="ACG8V3_002242"
     CDS             2308..3429
                     /locus_tag="ACG8V3_002242"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0300726.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3687..5189
                     /gene="eat(A)"
                     /locus_tag="ACG8V3_002243"
     CDS             3687..5189
                     /gene="eat(A)"
                     /locus_tag="ACG8V3_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0300727.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5256..5783
                     /locus_tag="ACG8V3_002244"
     CDS             5256..5783
                     /locus_tag="ACG8V3_002244"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0300728.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5994..7055
                     /locus_tag="ACG8V3_002245"
     CDS             5994..7055
                     /locus_tag="ACG8V3_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0300729.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7058..7954
                     /gene="murQ"
                     /locus_tag="ACG8V3_002246"
     CDS             7058..7954
                     /gene="murQ"
                     /locus_tag="ACG8V3_002246"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0300730.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7968..9950
                     /locus_tag="ACG8V3_002247"
     CDS             7968..9950
                     /locus_tag="ACG8V3_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0300731.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10015..10461)
                     /gene="nrdI"
                     /locus_tag="ACG8V3_002248"
     CDS             complement(10015..10461)
                     /gene="nrdI"
                     /locus_tag="ACG8V3_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0300732.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3450 a         1861 c         2280 g         2970 t
ORIGIN      
        1 tgataacaaa ttttcagaca attacaacta ttcagaaaac ttttaggcga attttaatag
       61 aagaatgatt tatttcatca tattttcata caaatgttaa attttcatac aaatatagga
      121 aacgttttac tggcttttta ctatttaaac aggctatcat aggaaaaagt gacagagaaa
      181 gtgaggcgga aaagatgaac aaaaatgacg tactgctagt tgcaaaaaat aaatcacttt
      241 attttttgcc ttccatccgc tctttagatg tttggtccgg aaaagattta ctacagtcgg
      301 aattcattaa atcagctaga caacagttgg caaattatca gctgatcgtt tttttagatt
      361 atggatttga tatggagatg gctcgacgcg tgcgcccatt taccaatgca aaaattgtgt
      421 tatttttttg gaatcacttc aaagcagaac acgaggcgat gctgaagcag gcacaagatg
      481 aaccggcaat cgatgagatt tatcattttg attttttgga agcaaaagaa cttgggctga
      541 aacataatag ttcattttat tcgaaacata tgaatttttc ttctagcgaa ccaaagacgg
      601 atctattttt tggtgcaacg aataatggga gaaaagaacg agcagaaagc tataagcagg
      661 aatttataaa aagagatgtt acgaccaact atttcattct tccacatcga gggaatgaac
      721 aacctggata tcttacttat gaagagtacc tgaaaaaaac aggggaatcg cgcgggattc
      781 tggaactgtt gcgggagggt cagcaaggag tgactttgcg gacatttgaa tccatgtatt
      841 ttcaaaagaa actagtgacg gataatcaag cgattgctca ttatcatttt tatcgtcctg
      901 aaaatatttt tttgcttcga gaacgtgatt tagatgaatt accggaattt ctgcatacac
      961 catttaagcc aatcctatca gagctattgg aattttatga tgcagaaaat tgggcaaaac
     1021 gatttatcac taacgattcc actatttatg agcaatacga atatgacctc tcctctgttg
     1081 aaaacaaata aaaaacctac attttgaaga gattatgtac tgaaacaaac aagcacaatt
     1141 cttatggcat catgagagtt gtgttttctt tttgatcttt tattgggctt aaaaaaagag
     1201 aaccagctgc tgattcagct gattctcatc agagagcctt ttattctgcg ccaaggattc
     1261 cttcttttgc atggactaat tgggtcaaaa aggcacaata tggtgtagca atatcatatt
     1321 tttgtccttt tcgccaaatg gcaccattga tgtagtcgat ttcagtcaaa cgatggtttt
     1381 tgatcaggtc ttggtacata gatgggtaat gcaacccgat gttgtcagga tcataagtag
     1441 aagcaatatg ttggtacact tcttcttgat ctagtatgat tccttctttt gctgcgatag
     1501 aagcaaattc agatacgatt gttcgaacca tgctctctgc cgctttttgc gcgcctagtt
     1561 cagcaatatt acaatccaaa atcgtgcata aaccattcaa tgttccattt acacaagctt
     1621 ttcgccaaat ggaataacga acgttatggc tatagatcgg atttaaattt gcttcttgaa
     1681 agacttctac aacttttttt gtaaatgctt cgccttctgg ttccaagttc tctagttcga
     1741 tttctccatc acctaacaat gtgacttttc cgggaccagc tagaccagct gtccacatcg
     1801 tgatcccaaa taagatattt ttctttggca catatttttc cagtacatct tcatgaccta
     1861 atccatttag taaacacaaa acataagtat tgtcagtaat aatggattgg atcgaacaaa
     1921 acatatcatc taattgattg gcttttgtta atgctacgat caagtccacg tgttcgttgc
     1981 tttcgatgat ttcttctggt gaatagattg gcagttttgc gaccacttct ttaccgttaa
     2041 agtcagcaat caacccattt gtacgaatgg cttcaatgtg agcaggccaa cgatcgatca
     2101 agaggacctc atttccgctt tgatgtagca tcaatccgat tcggctgccc attgctccgg
     2161 ccccagctat tgcgattttc atgttttttc tctcctttat tcaatgagtt cccttttagt
     2221 atatagaaaa atggaagaaa atagcattgt tttgtttgaa aaaaatgaaa aaaagtgtct
     2281 tacttaagaa gaaagtgagg gatacagatg gatattttgg cggcaattga ttcatttaaa
     2341 ggaagtgctt cttcatttga actaaatcag gcagttctaa atggctggga agatcgtaaa
     2401 ggaagaaaaa tcaatgtgcc gattgcagat ggcggagaaa atactgcaga agcaatctat
     2461 tacgcgctca ggggaacatg gcgtaccatt gatgggatgg atctcttatt ccgtcagcga
     2521 aaaatcagct atttattgac aacttggcaa ggaaaaaaca ccgcagtcat cgaatctgca
     2581 caagcattgg ggttagatct acttgataaa ccaacggatc aaactatccg acttgcttcc
     2641 agttatgcct taggagaact tattaaagat gcactgcttc aagaagtaga acaaattatt
     2701 gtcactttag gaggaagcgg gtgtagcgat ggtggtctag gattactcca atccttgggt
     2761 gcttctttag aagggtacac agaaggaaat cctttattat ctactaaaaa acttcatttt
     2821 gatcgaagaa acgttcttgt ttctaaagta aaattgatta tcgctgcaga tgttaccaat
     2881 ccatatacag gtcaacaagg agctgcttgg caatttggaa gacaaaaagg aggaatagat
     2941 acgacgctag ctttcttaga taaacaagca ggaaagattg ctcagcaaat aaaagatttc
     3001 tgggaggtgg atttagatga tattccagga agtggagcag caggcggtat tggtggagcc
     3061 ctttttttgt tgggagcgaa gatggtttct ggtttcgatt tagtcagcca aatcactgga
     3121 ttggaaaaaa agatcgaaca agcagatgtc atcattacag gagaagggcg gatcgatcag
     3181 caaacgatcc acggaaaagt tccatacggt gtagcagtgc ttgcaaaaaa atatcgaaaa
     3241 ccaataatcg ctatttgcgg aagtagagac caagagctag aaggactttc tgattttctc
     3301 ccaattgtct taagtattca tttgggtcca ataggtttag aagaagcaat ggaacacagg
     3361 caaacattag aaaaaacaaa aatcttagga cagacactta gcagattttt caccatcctc
     3421 ccgaagtagt tgacaaaatc attttcttta tgctatcgta atttcaaata taggacaaag
     3481 tgagggatcc gtgcattgaa aaattaagtc gattgatgaa agctgcattc agactgtacc
     3541 ttgtggagac aaggaagcag tgtgcagatt ttcgggattg gcttggtttt ttcgatgtat
     3601 gggtcttttt gtgtgtatcc aacccttgca tcctctttag ccagttctga aaataggact
     3661 ggtctttttt tacgaggtga tggaaaatgt ctaaaatcga aataaaaaat ctgacattcg
     3721 gctacgacag ccaaggcaca ttattatttg aacaagcaaa tctaaatttt gacacacaat
     3781 ggaaactagg acttatcgga cgaaacggtc gaggaaagac aactttactg aatattctac
     3841 aaaacaaact accttatcaa gggcaagtaa tccatcagca agaatttgcc tatttcccgc
     3901 aacagacaaa agataaagaa cgtttaacct attacgtgtt aaatgatatt acggattttg
     3961 agatatggga aatcgaaaga gagctccaat tgatgcaaac agatcctgaa atcttatgga
     4021 gagaattcag cacactatcg gggggagaga agacaaaagt cctactggca cttttatttg
     4081 tggatgacac tcatttcccg ttaatcgatg aaccaacgaa tcatttggat atctctggta
     4141 gaaaacaagt agcggcttat ttgaaaaaga aaaaacaagg cttcatcgtg gtcagccatg
     4201 accggggatt tatcgatgaa gtagtggacc atgttttagc aatcgaaaaa agtcaactgg
     4261 aactttatca agggaatttc tctatctatg aagaacagaa aaaacttcgt gatgaatttg
     4321 aaatggctca aaatgaaaaa ttgaaaaaag aagtcagtag gctaaagaaa acagcagctg
     4381 aaaaagccga atggtctcgt tcccgagaag gagataaaac aaagaaacaa gtcggattca
     4441 tcgatactga atctagacga gtgaataaag gagcagtggg tgctgatgct gcacggacga
     4501 tgaaacgatc caaagcaatc gtgaatcgga tggagaccca gatcagcgag aaagaaaaac
     4561 tattaaaaga tatcgaatat atcgattctt tgacgatgaa tagccaagcg tctcaccata
     4621 agcgactttt aagcgtagaa gatcttcaat tagggtatga aaatctgtta ttcgagccaa
     4681 ttcattttac aatcgagcct catcagcggg tggcgatttc aggtcctaac ggtgcaggaa
     4741 agtcatccat tatccattat cttctggggg cattcaacgg caaggttata ggagaaaaat
     4801 accagccaaa acatctgagc attagttatg caagccaaaa ttatgaagac aatcgaggaa
     4861 cgttggcgga atttgcagag aaaaaccaag tagactacca agcatttttg aacaacctcc
     4921 gaaagcttgg gatggaaaga gatgtttttc ataacaagat cgagcagatg agtatgggcc
     4981 aacggaaaaa agtggaattg gctaaatctt tatcacagcc agctgaacta tatacatggg
     5041 atgaaccatt gaattatttg gatgtcttca atcaagaaca attagaacaa ctgatcttga
     5101 acgtgaaacc tgccatgtta ctagtggaac atgatcaaac cttcctggat aaagtatcta
     5161 ctgagattat ttctcttgag agaatctaac ccttgtaatc tcaagaacaa caaggtatgg
     5221 ttaaggggaa ctttgccaac gaaggagaga atcacatgct ttatgtaacg agacacggag
     5281 aaacaacttg gaacgcacaa ggattagttt gcggacgggc agatgtgccg ctgactgaaa
     5341 aaggacagat gcaggcacag aaactagcag aaaaggtagt agatttgcct gttccaatca
     5401 cgaaaatcat ccattcccct cttcaacgag caagagatac agcacaagca gttgctgatc
     5461 gtctcagctt gccgctgacc gttgatgaac gattggtaga aatggatttt ggggattatg
     5521 atggactgcc aagcaaagat gagaactttc aaaaagcacg tttagcgttc gctgtacgat
     5581 tcccaaatgg ggagtcagtc ttagatgtgt atgcacggat cgttccttta ttgaaagaat
     5641 gcatcgaaga cgaagaaaat gtctatctgt tagtttgcca taatgccttg ataagagtga
     5701 tcaatgctta ttttcatccg atgcctaatg aggggttctt tacattcatg gtagataata
     5761 cagaattggt ttcttatgag taattatgtt tttgtgttat ttaaataaaa tatttcaagg
     5821 cactgctttt gcttagcagt gcctttttta ggttatgaca attcttttga taatataaac
     5881 tcaaccttta gaaatgaaat ttaattattt taataaaagt attgaaattt tgtttcttta
     5941 gatatatact ctagtcatag aaatgaagac ggatacagaa aaagagggat aatatgtttg
     6001 gcttttcaat atttatgaac gattctttgt cagaagagaa gaaacagtat atccgccaaa
     6061 tggcatccaa tggctttgtc ggtattttca cttccatgca tataccagaa gatgatgttt
     6121 cggcatatca aaagcgattg atcgaactag gaagcgaagc gaaaaagaat aaactagaat
     6181 tgatggtcga tatttcagga gaggcattgg aacgagcggg tttttcatta gaagatctaa
     6241 cgcctttgaa agcaattggt gtaacaggtt taagaatgga ttatcatatt tccaatcagc
     6301 agattgccga ctggtcacat caattgaaaa tcagtttgaa tgctagcacg atcacgccta
     6361 aagatatcga tgaattgaaa gaagcagaag ctgatttctc acagattgaa gcttggcata
     6421 attattatcc acgtcccgaa acagggctgg acaaagaatg gtatcagaaa aaaaatcaat
     6481 ggctcaaaat tcaaggtctc cttgtccaag gttttgtacc aggagataca gagttgagag
     6541 gcccactata tcaaggattg ccaactttgg aagaacatcg agggatccat cctcttgcag
     6601 gcgctctaga tctattagca agtaacacgg atattgttta catcggagat gcaggtctgt
     6661 cagaaaacgt tcaggagcaa tttgcttctt tccaaaaaga acagacagtc ttgctccata
     6721 cggaacctgt agatgaagaa ttttacgaat atatattagg gaagcatacg aatcggcaag
     6781 acgatgcaag agatgtcata cgaagcgtag atgcacgttt tcgagagatt ccgcctatcc
     6841 cagcaagaaa tacagctacg cgtatgaaag ggtctattac tttagataac gaaaagtatt
     6901 tgcgctatat gggtgagatc caattaacca aatatgattt gccagcggat gaaaaagtga
     6961 atgtagtagc taaagttatc aaagaagagc taccattgat caaccaaatc aaagctggta
     7021 tgaactatca atttataaga aaagaaggaa gataaaaatg agcaaaatca atttagagaa
     7081 cctaacaacg gaacgcagaa accaagaaac ttttggatta gatgagatga gtatttctca
     7141 agccgttcaa ctaatgaaca aagaagatca gaaagtggcc gctgcggttg aaaaagaact
     7201 gacaagtatc gaaccagtga ttgctaacac gattgagtcc tttaaaaaag gtggacgtct
     7261 gatttatatg ggagccggaa caagtggaag actaggtgtg ttagatgctg ctgaatgcgt
     7321 accgacattc ggtgtagaac cggaaatggt cataggtcta attgctggtg gagaagaagc
     7381 aatgactgta gctgtcgaag gggccgaaga ttcttatgag ctagggcaag aagatctaat
     7441 caagctgaat ttgacaaaaa atgatatagt tattggtatc gcagctagcg gtcgtacacc
     7501 atatgtgatc ggtggattag attacgcaaa atcgatcggt gctgtcacag gaacgatttc
     7561 ttgcaataaa caagccgaaa tcagtaaaca cgcggatttc ccaatcgaag tcgatgcagg
     7621 tccagaattt ttgacaggct ctactcgatt gaagtcagga acagcacaaa aacttatctt
     7681 gaatatgatt tctactatca gtatgattgg tataggtaaa gtattcaata acttgatggt
     7741 cgatgtcaaa ccaacaaatg aaaaactaat cgaacgctca aaacgaatca tcatgcaagc
     7801 gacagaagct gattacgaaa cagctgaaaa gtactttgaa gaagcagatc aaaatgttaa
     7861 gctggctatc gtcatgattt tgacaaatag tgataaagag gaagctcaag caaaattagc
     7921 tgaagcaaac ggatttgtaa aagggacctt atgaggggga aaagaccatg gcaaaaaacg
     7981 aactgacttt ggaagaactg gcaaaacaaa tttataaagg ggcaggcggc atgggcaatg
     8041 tagaaagcat tgtccactgc atgactcgtg tgcgtatgaa gatcatcaat gatgatcttg
     8101 taaatgtctc tgaattaaaa gcgattccag gagtgcttgg tgtagtagaa gatgaacagc
     8161 ttcaagtcat catcggacct gggaaagtga acaaagtagc acagcaaatg gttgatttgg
     8221 caggcgtgaa attaggagaa aaactttctt ctgtaagtac agctacagca aattcatcag
     8281 catctggaaa agaccgtgta aacgaacggg cacaagaaat gaaagcggca caaaaatcga
     8341 aacaaaaacc atctaaaatc aaatcggtat taaaagacat ttctaatatc tttgtgccaa
     8401 tgattcctgc atttgtcggt actgggatcg tggcagggat tgccgcagtt ttagcaaact
     8461 tagtcacagc cggaacactt gatgcagcaa catggcaaca atatatcgat gtgatgaaca
     8521 tcttgaaaaa cgggatgttt agctacctag ttatctatac aggtatcaat gctgcacaag
     8581 tttttggtgc aacaccaact cttggtggag ttatcggggc cgttgtgatg ctgacaggga
     8641 tgaatccgga agcaccactg aaaaacttat ttactggtga agctcttgca gctggacaag
     8701 gtgggatcct aggtgtcatc ttcgctgttt ggctattgtc aatcgttgag aaaaaactgc
     8761 ataaagtagt acctgatgcg gtagatatca tcgttacacc aactctttca ttgattgcca
     8821 tcggtctgat tgaaattttc ttcatcatgc ctttagcagg tttcatttct gatggcatgg
     8881 tcggtgggat caactgggta cttggcgttg gtggagcatt tgccggcttc gttctaggca
     8941 cattattctt gccgatggtt atgttcggac tgcatcaaat tttgactcca atccatgtgc
     9001 aaatgattga tgaaactggt cgtacgctat tgcttccaat tcttgcgatg gctggtgcag
     9061 gacaagtggg tgcagcattg gcattatgga ttcgctgcaa aaaagataaa gaattaacag
     9121 aaatgatcaa aggagcttta ccagtcggga tcctaggaat cggcgaacca ttgatttacg
     9181 gagtaacatt acctctagga cgcccattta ttactgcttg tatcggtgga ggtatcggcg
     9241 gggcagtgat cggaatgatc ggaaatgtcg gggctatcgc tattggacct tcaggagccg
     9301 ctttgatccc attgatctct gacggtaaat ggttaggcta catccttgga ttattggcag
     9361 cttatgctgg tggcttcgtt gctacattct tcttcggtat tcctaaagac cgtttagaaa
     9421 aagaagaatt agcgactgaa acagccacag taaatgaagt gcctgaaaaa gtagtacctc
     9481 aaacttcagc tgaaagtgtg actatcctct catcagttgc tgacggaaca gttgtttctt
     9541 tagataaagc aagtgatcca gtttttgcac aaaagatgat gggtgaaggc tactttgtag
     9601 aaccagaaaa cggacatatc tattctccag ttactggggt agtcagctct gtcttcccaa
     9661 cgaaacacgc tattgggatc acaacgccaa atggtttaga aatcttgctt catatgggaa
     9721 tcaacacagt tgacttaggc ggtacgccat ttgatgtgaa agtaacagaa gggcagcaag
     9781 tgacacctga tacgcttgta gcagaagctg acttagaagc gatcaaagca gcaggcaaag
     9841 aaacatccat gatcgtcttg gtaaccaata tggatcgtgt aaaaaacttt gtcttggaaa
     9901 aaacaggtaa agcaaaagca aaagcacctg tgatggatgt caaagcttaa tatgatagaa
     9961 acaatagaaa cgaaagaaga aactgaaatc tatttttcag cttcttcttt tttattattt
    10021 attgttttct gctaaaatct gataaattcg tgtcacatca gatggtgttc cccgtaattc
    10081 ataatctgcc aagaaaggaa agccaaatgt ttgtgcatat tgtttggctg ttagacaata
    10141 ctgattattg aagtttttat ttccgctacc gactacacca agacaaagtt tatgattgtc
    10201 ttcatgttcc agatattcac gcatcgtttc ggtcaagatt tctgtatcac cgttatctaa
    10261 accatttccg ccgtctaaat aagtaggaac gaaagtaaaa aatggttctg tttctttcgt
    10321 aaaatcacta ttttcatgga tttcttttaa agtaacttca ggattgacag gatcttctgc
    10381 atgcattttt tcagcatatt ctgctagatg tttggcaaag gcacgcgtat ttcctgaaat
    10441 cgatatatat aaaatgttca tggatcatcc tccaattctt tccttttagt atagcatctt
    10501 ttttaagttt tttggtggtg taatttggaa agggactagg cgctttgctt gtataatgag
    10561 a
//
LOCUS       DAZKMB010000102        10517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000102 DAZKMB010000000
VERSION     DAZKMB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.102"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACG8V3_002249"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACG8V3_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACG8V3_002250"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACG8V3_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACG8V3_002251"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACG8V3_002251"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0300733.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8V3_002252"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8V3_002252"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0300734.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACG8V3_002253"
     CDS             5704..6489
                     /locus_tag="ACG8V3_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0300735.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACG8V3_002254"
     CDS             6502..7353
                     /locus_tag="ACG8V3_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0300736.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACG8V3_002255"
     CDS             complement(7477..7929)
                     /locus_tag="ACG8V3_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300737.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="ACG8V3_002256"
     CDS             complement(8394..8762)
                     /locus_tag="ACG8V3_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0300738.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="ACG8V3_002257"
     CDS             8912..9394
                     /locus_tag="ACG8V3_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0300739.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="ACG8V3_002258"
     CDS             9431..10441
                     /locus_tag="ACG8V3_002258"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0300740.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         3576 a         1748 c         2192 g         3001 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaa
//
LOCUS       DAZKMB010000103        10107 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000103 DAZKMB010000000
VERSION     DAZKMB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.103"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..262)
                     /locus_tag="ACG8V3_002259"
     CDS             complement(65..262)
                     /locus_tag="ACG8V3_002259"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0300741.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(408..1286)
                     /locus_tag="ACG8V3_002260"
     CDS             complement(408..1286)
                     /locus_tag="ACG8V3_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0300742.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1325..2443)
                     /locus_tag="ACG8V3_002261"
     CDS             complement(1325..2443)
                     /locus_tag="ACG8V3_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0300743.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2440..4539)
                     /locus_tag="ACG8V3_002262"
     CDS             complement(2440..4539)
                     /locus_tag="ACG8V3_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0300744.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4590..5411)
                     /locus_tag="ACG8V3_002263"
     CDS             complement(4590..5411)
                     /locus_tag="ACG8V3_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0300745.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5408..6217)
                     /locus_tag="ACG8V3_002264"
     CDS             complement(5408..6217)
                     /locus_tag="ACG8V3_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0300746.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6238..6732)
                     /locus_tag="ACG8V3_002265"
     CDS             complement(6238..6732)
                     /locus_tag="ACG8V3_002265"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0300747.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6744..7244)
                     /locus_tag="ACG8V3_002266"
     CDS             complement(6744..7244)
                     /locus_tag="ACG8V3_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0300748.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7309..10005)
                     /locus_tag="ACG8V3_002267"
     CDS             complement(7309..10005)
                     /locus_tag="ACG8V3_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0300749.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3072 a         2272 c         1659 g         3104 t
ORIGIN      
        1 ctttttataa tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa
       61 tctatcacca aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa
      121 tgaacataca ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat
      181 tactatttaa gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag
      241 aaaaaaggaa acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac
      301 ttagagcctt tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt
      361 actcggttaa atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta
      421 tttcttaccg cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat
      481 tcgggcgtaa taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca
      541 acaaatttat cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa
      601 ataattctat ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag
      661 gcttcaatct gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt
      721 ttttcaatcg ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt
      781 cctgaaaaat tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg
      841 aacccttcca cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg
      901 aagtatgcaa cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc
      961 gcattgacat ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca
     1021 aaattgaaat tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat
     1081 ttgcattgaa tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta
     1141 aaaggtttaa aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa
     1201 ctgactcttt ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct
     1261 ccagcttcaa ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac
     1321 ttcattaacg cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt
     1381 ctgttgaata ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat
     1441 catcgttgcc gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt
     1501 gaagatgcca acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg
     1561 cattcaattt tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat
     1621 accctaaaaa ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat
     1681 gaaaggccca cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact
     1741 cactatcccc atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc
     1801 aggtaagcgc aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact
     1861 tataatcctc ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc
     1921 tattttcttc caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat
     1981 ccttcggatg taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat
     2041 aggaattaaa gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct
     2101 ctccaaaccc attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc
     2161 catcccaata aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat
     2221 cacgaatcgt catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag
     2281 tttcttcacc agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc
     2341 ttggcaacat cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac
     2401 tctggatttt tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat
     2461 gtgaatcgtt tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg
     2521 cttttctgac aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc
     2581 ttgaccgaac ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa
     2641 tactcctttt ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc
     2701 cgatgcccag ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc
     2761 taagcgatac ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc
     2821 taatcgggtt acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc
     2881 aggaaaagtg gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt
     2941 tggattgaaa gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc
     3001 taaaaattga taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa
     3061 attaattttt ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg
     3121 tttttgatag tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa
     3181 ttgctcacca aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca
     3241 ggcaatgttt ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc
     3301 aagtccaata caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc
     3361 aaaggcgtct gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc
     3421 taatatgtcc tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa
     3481 ttttgtatcc ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt
     3541 actcagagaa tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg
     3601 atcaaaccat agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga
     3661 tgcgtaatac atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct
     3721 gccgacgacc tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg
     3781 acgatagaga tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa
     3841 atagttcaat gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc
     3901 ttcaaaagca tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat
     3961 cgtgtcctta tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg
     4021 atacgaaggc acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta
     4081 aacctgttga acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac
     4141 aatgacctta aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc
     4201 gacttcaata cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt
     4261 taaggataac gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc
     4321 agttagtaaa ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt
     4381 tgctaatgtc ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg
     4441 atagtagctg tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa
     4501 tgggtgtttg ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa
     4561 aactgcagcc tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt
     4621 cccaagacga ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg
     4681 aaacagctca atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct
     4741 tggacattca ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc
     4801 atactgatcg tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga
     4861 atttcaatta acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct
     4921 aaaacgccgc catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata
     4981 attggtgcca aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca
     5041 cgccaaactc cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt
     5101 ttgatcgcgt tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc
     5161 gatgccgcaa tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta
     5221 tgacgttcca gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc
     5281 atcatagaat aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata
     5341 gtcatcgagc gccaaaacat cttccgaacc aattttttct catctttaga aattccatca
     5401 gctaatttca ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat
     5461 cgctttgaac tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa
     5521 ttgcaataat cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa
     5581 caacggcccc aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca
     5641 tcgccatccc aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct
     5701 ctggaatatt atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg
     5761 gaataagagc ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc
     5821 ctttttgatc accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa
     5881 aagaaaaaat catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa
     5941 taatcgcatc cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca
     6001 aagaaggctc cgccgtacta gagattccga tattgacgac acccataaag atggcttcta
     6061 gtgacgcccc gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag
     6121 caacaacaat cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa
     6181 taaaaacggc caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta
     6241 aaaatcactc aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac
     6301 ggtatctgga taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat
     6361 cacttccttt gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc
     6421 cacgttcatt aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac
     6481 attagggact aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat
     6541 cgcgtcatca actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt
     6601 catcttttga atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt
     6661 tagactcttt gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac
     6721 tgctttaatc atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc
     6781 tcatcaagaa tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc
     6841 tcctttgtta attccttggt tgccaatact atttcgagca gaattggcag attaaagcca
     6901 gacaaaatag ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca
     6961 cttccgccaa ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca
     7021 aatacattca actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc
     7081 tcttgaacct tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg
     7141 tgagtcgcta ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata
     7201 taagcaattc ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa
     7261 aaaagtgtct aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat
     7321 atacggtcct aataattcat agagcagcga aagctcataa gcattgactt taaaattata
     7381 tttcatctcc attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg
     7441 tttcaaatca taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat
     7501 catcaatgcc gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata
     7561 gtaattttca tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag
     7621 aaagttcaaa cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa
     7681 gcggttaaat tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc
     7741 atcaccgtct aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc
     7801 cgaagtcgtg atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa
     7861 ttcacgtagt tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg
     7921 acgaaaaaca tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat
     7981 gttgctactt tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc
     8041 atcctccgca atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc
     8101 aatggaagtc gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg
     8161 tgtgtaaatt tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac
     8221 ttgccgtttc acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg
     8281 catatccaac gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct
     8341 gctagccgta ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac
     8401 ataatcagat aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag
     8461 cggtggtaaa tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc
     8521 taatgtacga tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct
     8581 gctaataatt tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga
     8641 tacccagacc tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc
     8701 cttatcaatg tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt
     8761 gaaattacct tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg
     8821 tttgattcga tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa
     8881 gctttctact tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt
     8941 taatggttct tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt
     9001 cagcttacca atattcccac taaagggatg agataacatt aaagacaagg cttctcttga
     9061 aacaataata tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc
     9121 taatcgttct cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg
     9181 ccgatcaaag gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg
     9241 aaccttagca gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat
     9301 aaatagaaag agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag
     9361 atagccgttg ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc
     9421 ccccttcttg tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc
     9481 acagtttaag atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc
     9541 aaaaattttt gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact
     9601 gagtccgtta ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact
     9661 tccgtcatga ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac
     9721 cgcatattca tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt
     9781 atcttccact aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt
     9841 cgaaattttc tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa
     9901 aactcgatct ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata
     9961 ataaactatc ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac
    10021 acaaatagtc gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt
    10081 tttatctaat atgattttta tgttttt
//
LOCUS       DAZKMB010000104        10086 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000104 DAZKMB010000000
VERSION     DAZKMB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.104"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..366)
                     /locus_tag="ACG8V3_002268"
     CDS             complement(46..366)
                     /locus_tag="ACG8V3_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0300750.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="ACG8V3_002269"
     CDS             complement(514..2748)
                     /locus_tag="ACG8V3_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0300751.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="ACG8V3_002270"
     CDS             3227..3493
                     /locus_tag="ACG8V3_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0300752.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG8V3_002271"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG8V3_002271"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0300753.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5384..6430
                     /locus_tag="ACG8V3_002272"
     CDS             5384..6430
                     /locus_tag="ACG8V3_002272"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0300754.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6447..7670
                     /locus_tag="ACG8V3_002273"
     CDS             6447..7670
                     /locus_tag="ACG8V3_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0300755.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7759..8646
                     /locus_tag="ACG8V3_002274"
     CDS             7759..8646
                     /locus_tag="ACG8V3_002274"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0300756.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8732..8962
                     /locus_tag="ACG8V3_002275"
     CDS             8732..8962
                     /locus_tag="ACG8V3_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0300757.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9019..9984)
                     /locus_tag="ACG8V3_002276"
     CDS             complement(9019..9984)
                     /locus_tag="ACG8V3_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0300758.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3209 a         1784 c         2038 g         3055 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttg
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtagtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 acaacgtatc aaaagctggt gtggcttgta aaagagctac gccagttttt ttgttctttt
     5281 ttaagaaaac gtttttgtca gtaagaatag acaaaaatag ggcttaagtt tgagtgacag
     5341 tttgtaggct atgttatact atataagtga agggggacgt aaggtgaagg tattattata
     5401 ttttgaaagt gaaaagatgc ttgcaaaatc tggaatcggg cgtgcgcttg atcatcaaaa
     5461 acgtgctctg acggaagtcg gtatctctta tacactagat gaaaaagaag attatgacat
     5521 cctgcacatc aatacatacg gaatcaatag tcataatatg gtcaataaag ccagaagaga
     5581 aggaaaaaaa gttgtctatc atgcgcattc tacagaggaa gatttccgaa attcctttat
     5641 cggatcgaac cagctttctc ctatcgtaaa aaaatatctg gtaggattgt accaaaaagc
     5701 ggactatctg atcacgccaa caccttattc aaaacagttg ttggaaagct atggtatccg
     5761 tgtgccgatt caagctattt ccaatgggat cgacttagag aaatatcacc ctgatccaat
     5821 gaaagaacaa aagttcagag aatatttcaa actgtctccg gatcaaaaag taatcatttg
     5881 tgtcggtttg tttttcgaac gtaaaggaat cattgatttc gtagaaattg ctaagaagat
     5941 gcccgaatac acatttattt ggtttggtca tgttcccatg tactcgattc ctcggaacat
     6001 ccggaaaatt gtaaaagaag atcatcctga aaatgtcctg tttcctggtt acattcgtgg
     6061 agagatcata gaaggtgcgt attcaggtgc agatctattc tttttccctt cttatgaaga
     6121 gacagaaggg atcgttgttt tagaagcttt agcaagcaaa caaaacgtac ttgttcgaga
     6181 tattccagtg tataatggct ggttagaaga tagcaagaac tgctatatgg ggaagacaaa
     6241 cgaagaattt atccgactga tccaacagat taccaatcaa gaactaccga gtacgacaca
     6301 agccggttat caaacagcaa aagagcgaag tatcaataaa atcggtgaag aacttaaaaa
     6361 tgtatacgaa agtgtactga atgaaaaagt ctctagcaaa ttaaaaaaag taaatcaggt
     6421 caaggactag tttggaggcg aaaaacatga aaatcggctt ttttaccgat acctactttc
     6481 cgcaagtcag cggagttgct acatcgatca aaacattaaa acaagaatta gaaagacatg
     6541 ggcatgaagt ctatattttt acaacgaccg atcctaatgc ggataagttg gaaaaagatg
     6601 tgattcgaat gccaagtgta ccatttattt ctttcaagga tcgaagagtt gttgttagag
     6661 gcatgtggta tgcttatttg gttgccaaag aactagaact agatcttatt catacccata
     6721 cagaattcgg agcagggata cttgggaaga tggtagctaa gaagttgaaa atacctctga
     6781 tccatactta ccatacgatg tatgaagact accttcatta tatcggtaaa ggaaaagtga
     6841 tcaggcaatc ccatgtaaaa tacttgtcta gattatttgc aaatcatacg acaggtgtag
     6901 tttgtccaag tgaacgtgtt attgatacat tacgcagcta tggtgtaata gcaccgcttc
     6961 gcgtgattcc tactggtatc gacattgaaa aattcaaacg ttctgatatc actcagcagg
     7021 atatcagcaa attaagggag tcactaggtc ttcaggaaaa ccatttgatg attctttctt
     7081 taagcaggat ttcttatgag aaaaatatcc aagccttgat taatgggttt cctcaggtca
     7141 tctccgctta tcctaacgcc agattagtta tcgtaggaaa aggaccttat gtagaagagt
     7201 tagaggaatt gatctcagaa ctgcagttag aagaatttgt gcagttcaca ggagaagtgg
     7261 acaacaatga tgtagcactt tattataaag cggcagatta ttttgtcagt gcttctactt
     7321 cagaaacaca agggcttact tatacagaag cgatggcagc cggaacacct tgtgtcgtgg
     7381 aaggaaatgc ttatttgaac cgattgttcg atcatccttc attagggaaa acttttgaaa
     7441 ccgacgaaga ctttgcacca gcacttattg attatatttc cagtgaagtt cctaaagatc
     7501 caaaagtttt agaagctaaa ctttatgata tttcatctac acatttcggc gaagctatga
     7561 tcgaatttta tcaagacatg gttgtttatc atgaagaaga gatgcgaaga aaagaagaag
     7621 aagactcgat tgaacgaatc aaaataaaat taaattcatt taaaagataa aattcaaaca
     7681 ttttttatga aaaaatcctt gtttctagcg tatactagca tagaagcaag gatttttgat
     7741 ttttaggagg tcgaaaaaat gaaacttggt tttatcggaa caggcgtcat gggaagtgct
     7801 gtcgcacgcc atctactaga agcaggacac gaagtagctg tctataatcg gacaaaagca
     7861 aaagctgatc ctttagtaac agaaggagct atctgggcag atacaccaaa agcggtagca
     7921 gaacaaagta atattctctt tacaatggta ggttacccca aagatgtaga agaaatctac
     7981 tatggacagt cggggatttt ttctgcagat atctcaggac atattttagt tgatttgact
     8041 acaagtacac caagcctggc agaaaaaatt gcaaaaacag caaaagaaaa aggcgcagat
     8101 gcgttagatg caccggtatc cggtggtgac ttaggagcaa agaatggtac actgacgatc
     8161 atggtcggtg gagaagaagc cgtttatgat caagtactgc ctttattcaa agaatttggt
     8221 acgacattta cattgcatgg ttctgctggg aaaggtcagc atacaaaaat ggctaatcag
     8281 atcatgattg caggtacgat gacaggaatg acggaaatgc ttgtctatgc acaaaaaaac
     8341 ggacttgatt taaagaaagt gattgagacg ctttcaggtg gaagtgcagc taactggtct
     8401 ttaagcaatt attctcctcg tattttaaaa gaagattaca cacctggttt ttttgtcaaa
     8461 cattttatca aagatttaaa gattgcatta gaagaagcag aaaaaatgga tcttgtattg
     8521 ccagcaacta cccaagcatt gaaactatat gaagagttgg cagataaagg ttttgagaat
     8581 gacggaaccc aagctttgat caaactttgg tggcctgaag gaaaaatacc agaaaaaaaa
     8641 tcataaaaaa cgagtttttt tagagaaaat caatgcaagt aggctatgct tttgatacaa
     8701 tataacagaa ataagaaaag gaggacctcc gatgacacat tcacaattag tcgcaatcat
     8761 aaaacgactg gaggctatgg tcgagtcagc agataatgaa ctgcaagttc gccgttttga
     8821 aaaagaaggc gtagagaagt gtacagtaac ttatgataaa gcgaccgata catttgaatt
     8881 aactgaaaca gatactcatc agcaatacga atttgataat atcgatatcg tagcgatgga
     8941 aatctatgac ttaatccaat aaattttagg ataattgaca tttgaagttg aagatgccta
     9001 aaagaagccg agaagcaact aatttagttg cttttcagct tcttttttgt ttaacagttc
     9061 aatttcaaac tgttcttttt ctaatttttt gcggtaactg atcaagattt gtacaggatc
     9121 gatttcttct tttgacaatt gacgtttcaa agataaatga gcttctttct tttcataata
     9181 gaagtcagga gaaacgtcac cagaggtaat cattgtttct gcaagttgac cataacaaac
     9241 gaggtagtgc tttttctgac tctcatctgc tgctaatggc atgctgaagg tgagcgatgg
     9301 tgttcttaaa aactgctttt gttcctggat ccgtctaata aaataagtaa ctaattcgat
     9361 atctttttga agcataccgg ctttttcgaa attcaactga tagatgaaat gttgttgtct
     9421 ttttttcaag taagtcagta gctttttatt tcggccttga aacagccctt ggagttcctt
     9481 ttttttctgt tcaaaagtca tttcctgtag gtcaggaagc tggactcgca aagctaacaa
     9541 actaatttca gacaaccaag gcatttgata agtttcttcc attatacgca aaatagacgg
     9601 cattttttta tagagacgaa aaggcccgta tgttcgagcc gtaggagtat ctgttaatac
     9661 aaatccctta ttggtaatat gtacataatt ataattactg aaataattca tttgtcggtt
     9721 ataaaaagga cggtaatgtt ggatcaattg acactcgagc aataaagcat ctaattccgt
     9781 gtctactacg acgaaatcaa aatctgttat atgatgaatc atacgtaata ctttttttga
     9841 atgctgttta ttctgatgaa aatagctgct tactctattt ttgagccgtt ttgctttacc
     9901 aacatagatg accttaccat ctcggtcttt cataagatag acacccggca gcatggggag
     9961 agttttcatt ttttctttta acataggatc tccttttacg ctttgaataa aaagactata
    10021 acaaattttg aaataatgga caagttttag ggaattctat agttctgttc acagaaaata
    10081 ctttga
//
LOCUS       DAZKMB010000105         9811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000105 DAZKMB010000000
VERSION     DAZKMB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.106"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2154)
                     /locus_tag="ACG8V3_002277"
     CDS             complement(34..2154)
                     /locus_tag="ACG8V3_002277"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0300759.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ACG8V3_002278"
     CDS             2384..3562
                     /locus_tag="ACG8V3_002278"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0300760.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ACG8V3_002279"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ACG8V3_002279"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0300761.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG8V3_002280"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG8V3_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0300762.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ACG8V3_002281"
     CDS             complement(4915..6168)
                     /locus_tag="ACG8V3_002281"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0300763.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG8V3_002282"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG8V3_002282"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0300764.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ACG8V3_002283"
     CDS             7746..9092
                     /locus_tag="ACG8V3_002283"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0300765.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="ACG8V3_002284"
     CDS             complement(9400..9786)
                     /locus_tag="ACG8V3_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0300766.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DAZKMB010000106         9592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000106 DAZKMB010000000
VERSION     DAZKMB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.107"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..433
                     /locus_tag="ACG8V3_002285"
     CDS             11..433
                     /locus_tag="ACG8V3_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300767.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(599..1792)
                     /locus_tag="ACG8V3_002286"
     CDS             complement(599..1792)
                     /locus_tag="ACG8V3_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0300768.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2016..2393
                     /locus_tag="ACG8V3_002287"
     CDS             2016..2393
                     /locus_tag="ACG8V3_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0300769.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2381..2719
                     /locus_tag="ACG8V3_002288"
     CDS             2381..2719
                     /locus_tag="ACG8V3_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0300770.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            2853..3296
                     /locus_tag="ACG8V3_002289"
     CDS             2853..3296
                     /locus_tag="ACG8V3_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0300771.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3558..4373
                     /locus_tag="ACG8V3_002290"
     CDS             3558..4373
                     /locus_tag="ACG8V3_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0300772.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4383..5045
                     /locus_tag="ACG8V3_002291"
     CDS             4383..5045
                     /locus_tag="ACG8V3_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0300773.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            5250..6914
                     /gene="alsS"
                     /locus_tag="ACG8V3_002292"
     CDS             5250..6914
                     /gene="alsS"
                     /locus_tag="ACG8V3_002292"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0300774.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            6927..7637
                     /gene="budA"
                     /locus_tag="ACG8V3_002293"
     CDS             6927..7637
                     /gene="budA"
                     /locus_tag="ACG8V3_002293"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0300775.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            7767..8264
                     /locus_tag="ACG8V3_002294"
     CDS             7767..8264
                     /locus_tag="ACG8V3_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0300776.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            8449..8709
                     /locus_tag="ACG8V3_002295"
     CDS             8449..8709
                     /locus_tag="ACG8V3_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300777.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(9071..9310)
                     /locus_tag="ACG8V3_002296"
     CDS             complement(9071..9310)
                     /locus_tag="ACG8V3_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300778.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         3243 a         1580 c         1914 g         2855 t
ORIGIN      
        1 aggagaaaat atgcagactt ttttaacaaa agtttctcaa aagaaaatta aaatgattca
       61 tttactgctt gaaacagaac gttggtgttc aatagaagaa ctacaaaacg aattgaaggt
      121 atcaagtaag tctatcttga attatctaac agatttagaa gaattgttcc aaaaatatcc
      181 tgataaggtc attttgaaga atgagaataa tcggcgcttt tctatggaaa agcaggagaa
      241 cttcccaatc tacattattt atcttcattt ctacagaaaa tcatataatt atcatttgat
      301 tgaattcatg tatcaacatc ctgagaaaaa tttggaagat tatgcagacg ctcaatatac
      361 gagtgtatcc acagtttttc gttatgctaa gttattagta aagtactttg aaatgacgga
      421 cgtcaaagaa tagtcgccgc cagatagaag gaaattagca gttgaaaaag ctgccctgca
      481 tcaagtaata cgaccagcca aacggaaaca gtgtctcatg tttttgattt ggctggtctt
      541 attaccgcag atgcaagctt ttgccacacc tctttcttgt catttacaga ctcaactatc
      601 aatgctgcaa gctttccctt aatgctgtaa caccagtaag agttgtcaag accagcagac
      661 tgactaggta aataccgctc aaaaacaaga ttacggcagt taaactataa tgatccatca
      721 agaaaccgat gacgatagaa gaaaaaccac caacagctcg tccaaggttc ataatgaaat
      781 tattcgccgt cgcgcggatt tctgttggat acaaactact aatcactgcg ccatatccac
      841 catacatgcc attgataaag tagccgacta tcacagcggc tgctaccaaa ctccactggc
      901 tatgggctaa cacaagtaga tagacagata aggatgaaca aattaaaaac aaagaaaagg
      961 ctagtttagg ccccaattta tccatgatta tgccaaaagt catcatcccc aaagacatgc
     1021 ctaaaatagt actgaccatc cacaatgaag acccagaaac cgaaagccct aatcgatttt
     1081 gcataattga aggcagccaa ttcattaacc caaaatagcc ggcaatttgg acagttacca
     1141 tgatactaag gccacatgtt tgccaagcaa gatgttttga ttcaaacagt ctgctaagtt
     1201 tggttttctt ttcggcagat tgtacagatt tgaattcctt tggttcagtc aagccgcgac
     1261 gaatgaacaa gaccatcaac accggcagta cacctacgac atacaacatc ttccaaccaa
     1321 acgttggaat gatcacagca gctaaaatcg cagccaaagc cgcaccgacc tgcgcaccaa
     1381 taccagcaac cgaagaagct cgtccaattt gtttttttga aaaactttcg gaaatcaagg
     1441 acatacacgc cccgtattct cctcccgctc ctacacctgc gataaagcga aagagataaa
     1501 ccaagtagat gtttgaagca aaatacatca ataaagaagc aatcgaaaac agaaggaccg
     1561 tttgagaaaa tactttcaca cgtccatatt tatcagctat gatgccaaaa aaaatacctc
     1621 caatgagcat ccccaaatta gtgatcgttg aaatgaaacc cgcttgcgta ccactaatat
     1681 gaaaagaagt aatcattgaa gataatgaaa aagctaaaaa cataatatcc atattctcta
     1741 aagcaattcc cgccgatgtc gataataata cctttcgctg atacgttgtc atttttatac
     1801 cccttttcct aaaatttccc taaattgtta aaagtatatc caaaaaagaa taaaaaaaca
     1861 agactttttt atttgaaata atacacaaac aaccgatata gataatccag cttagtttta
     1921 atagaaatga gtattgactt ggagtacact taaagttgta gaataaataa aatacataag
     1981 taaagtggca agaaattaag agaaagagtg atcagatgaa tatcaaacaa gtcagtgaag
     2041 aaaagggcat ttcggccgat acattgcgct attatgaacg aatcgggttg attccaccag
     2101 ttaatcggac aaaaggcgga atccgtgatt atacagaaga agacctaaaa tgggtcgact
     2161 ttacgatctg tatgcgaaat gccggattat cgatcgagtc tttgattgaa tatattcgcc
     2221 tatctagcga aggagaagca actgtatttg agcggcgtaa gcttttgatt gaagaaagtg
     2281 aacttttgac gaaagaaatt gctgaaatgc aagagtgttt ggaacgttta caaggaaaaa
     2341 tcaagaaata tgatcgtgtg ttatctagaa ataaaatgga atgtatcaag taaaaaaatc
     2401 aaggagcatc gctatggagt tacgcttact aaaatatttt tggacaattg cagaagaagg
     2461 aacaatctct caagccgcag aagttctaca tcttacacaa ccaaccttaa gccgacaact
     2521 gaaggagtta gaagaggaac taggaacaga actgtttgta cgcgaaaacc gcaaaatgat
     2581 cttgacagaa gcaggttatt ttctaaaaaa gccgagcaga agaaatttta gatttaacga
     2641 atcaaaccac tcaagaattc gagaacagaa aaaaacaatt attcagcggc cgatttacga
     2701 tcggctgtgt ggaagctgat aattcgaata cattggcact attactagaa aagttgatcc
     2761 gtgattatcc cagcgttcaa tttcatatcc acagcggaac aagtgatgat atcacggagt
     2821 gactagaaaa aagcctgtta gatttagcga ttttgctaga tccagtagat acagagaaat
     2881 acgaaacgat tttgttacca agaaaggaaa aatggggatt gctcatttta gaaaattctg
     2941 ttttagcaaa aaaagacgtg ataacaaaag aagactttgc gaatgtacct cttttgatct
     3001 caaagcgtcc agaaatacag cgaatgtttg ctgactggtt aggcataaaa aaggagcagt
     3061 tgctgatcac aggaacgtac aatttgattt tcaatgtatt tgcgctaatt gagaatcagg
     3121 taggtgctgc tttagtcata gaaggtgctg taagtcatct ttcaaagcga gtgacattcg
     3181 ttccgcttga cccaatgatt gaaacgaact gcatgctagt ctggaaaaag aatcggattt
     3241 tatcacctgt agtcaatgaa ttgattcacc gtttcaaaga agcttttgaa cagtaataag
     3301 tatctctaat tgaagaaatt tcaatacaaa agcgagacgt atgacagtct tgcttttgta
     3361 ttttttcatt tttaaatcaa aaaaaataaa gagatttaca taacttgtta aataagcgct
     3421 tacaaacata agaagaacgg aaatgaatca gctataataa ccgttgttct ggattgtaat
     3481 cattacaaag aaatgaagat tataactttg gaggtgttcg aaatgaaaga aaaaaagcaa
     3541 aaattcgttc aaaaaacatg ccctttattc aatggttggg tatgattctg ctgattgtat
     3601 tagctttatt gacgatcatt ccttttttat ggatggtttc tgcttcattc aaagcaaata
     3661 atgaagtgtt cacgattccg attcaatgga tccctaaaac ttggcattta gagaattatc
     3721 tggttatttg gtctcgtatt cctttagtga cattttttaa gaatacagct tttttaagcg
     3781 ttgtgatcac gttgattcag ttgtttactt ctagttttgc tgcttacggg ttttctaaac
     3841 taaattttaa agggcgcgat attttatttt tagcatatat cggcacgatt gctgtccctt
     3901 ggcagtctta tatgatcccg cagttcatta tgatgagaaa agtgggttta acatatacgt
     3961 tgtggtcgtt ggtgctatta caagcgttca atgcttttgg tgtgttcttg ttgaagcaat
     4021 actacagtag tatcccagat tctttatgtg agagtgcgcg gatcgatggc ttatcagagt
     4081 ggggaatcta tcgcaagatc attttacctc ttacaaaacc ggcgattgcc agtttgacga
     4141 tcatcacatt cgttaataca tggaacgatt atatggggcc attcatttat ttgtcttcta
     4201 cggaaaataa aacaatccag ttagggttga aaatgtttat tgggctattt gatgcagagt
     4261 acgcattaat catggctgcc tcagttgttt ctgttcttcc tgttgctttt atatttttag
     4321 cgatgcaaaa atattttgta gaagggatcg cttcatcagg aatgaaggaa taggaggtga
     4381 ttatgaaaga gcagcgtttt gacaaaaatg tttatatgga attcttttat tggttgtatc
     4441 aatcattggc aagtagcctt tgtttttggt gtgtcaatat cttgtatata gcagctgcgt
     4501 tccttttgtc gatagatgga aggaatgtct tttcctttat tttttcttta ctttttattg
     4561 gtccaggttg catcgtgatg ataggtatga cagaagaatt agcaaaagaa aaaagttttt
     4621 caccagtacg tttgttcata aagacgtttc gccaattttg gctaaaggga tttttttatt
     4681 ggctatttgc ttggatagtt agtgtgataa tgatttttga ttgtttcttt tttatacgtt
     4741 tttcttatgg aaaatggttg attccgttgt ttgttttact tgcttgttta tcagtttcct
     4801 ttagcatcaa ctactggtat tttcaggtga gaaatccagc gtcgaaacca aatcaagttc
     4861 tacggatagc gttctactat acgttgaaaa aatggtatgt gagtttacta gattttttac
     4921 tattgacaag tttgtttctc ttctttttcg taaagcccca atggtgtatt ctactaggtc
     4981 cttctatcgt atttggtttg atttacttca acaatcgtaa attgatgaga actatggatc
     5041 tgtagacttt ataaattgaa actttcaatt aagaatgaca gtttttatgt aaaaactgtc
     5101 attctttttc attttttgaa acatatggct aaacaaatga aataaaacgt gctatacttt
     5161 tgaatgaaac aggtttagaa actgtatcaa ttatttgtga ttatttctaa ctaataatta
     5221 aaaataaagc ataaaaagga cgatgttcaa tggacgaaaa aatgaaacaa ggttcgaaat
     5281 ctgtagtaga aagtttgatt aatcatcatg ttgattatgt attcggtata ccaggtgcaa
     5341 aaatcgatgg tgtatttaat gaattagagg atcaaggtcc tgaattaatc gtgactcgtc
     5401 acgaacaaaa tgctgcattt atggcacaag caatcggacg tattacagga gagccaggag
     5461 tagttatcgc aacaagtggt cctggtgcta gtaacttagc tacaggtttg gtaacagcga
     5521 cagcagaagg tgacccagtt ttagcaattg ccggacaagt aaaacgaagc gatctattga
     5581 agttgaccca tcaaagcatg gataatgcag cactgttcca gccaatcaca aaatacagtg
     5641 cagaaatcca agatccagaa acaatttcag aagtgattgc caatgcttat cgtatggcta
     5701 agagttcaaa aaaaggcgcg agctttatca gtatcccgca agatgtagtg gatgcacctg
     5761 tccaagggaa tgtcatcaag ccattgagcg atcctaaatt aggttctgct tctgcagatg
     5821 atatccgtta cctagctgaa cgcattagag aagcaaaact accggtacta ttagtaggta
     5881 tgcgaggatc aagtgaaaaa gaaacgttag ccatcaggca attagttgga aagacagcat
     5941 tgcctgttgt agagacattc caagcagctg gagtgatctc acgtgaatta gaagcgcatt
     6001 tctttggtcg tgtgggttta tttagaaacc aaccaggaga tatgcttttg aaacgaagtg
     6061 atctagttat tgctattggc tatgatccaa tcgaatatga agcaagaaac tggaatgcgg
     6121 aaaaagatgc gcggatcatt gtgatcgatg aagcgccagc tgaaatcgat ccttttatgc
     6181 agcctgaaag agaattgatc ggtgatatct cagccactct tgatttattg acaggttcat
     6241 tagagcctca acaagtgtca gaagatgcaa aagaatattt agcatctctt caagcagaat
     6301 taacggagcg ggacattgtt caatcaaaag gagaagcagg tatcttgcat cctttagaag
     6361 ttattaacac gcttcagtca aaagtaacag atgatatgac tgttaccgta gatgtaggta
     6421 gtcattatat ttggatggca cgccacttta gaagttatga acctcgtcat ttgttattca
     6481 gtaatggtat gcaaacgctt ggtgttgcat tgccatgggc gatttctgct gcattggtgc
     6541 gtccaaatac acaaatcgta tcagtttccg gcgatggggg atttttattc tctgcccagg
     6601 atttagaaac ggcggtacgt aaaaaattga atatcgttca tttgatttgg aatgatggac
     6661 actacaatat ggtagagttc caagaaaaaa tgaaatatca gcgggcgtca ggtgtagact
     6721 ttggtccagt tgattttgtg aaatatgcag aagcttttgg cgctaaagga atccgagcta
     6781 caagtgtaga ggaattagaa aaagctttag aagaaggatt tgcgacagaa ggacccgtaa
     6841 ttattgatat tccgattgat taccgtgata atgagaaatt gggagaaaca atcttaccag
     6901 atcaattcta ttaaggaagt gtcgacatga ctgaaaaaat cttataccag cacgggacat
     6961 taggagcttt gatggctggc ttgatggatg gaacagaaac gattgctcat atattagaaa
     7021 aaggaactct gggattggga acattgcatg ggcttgatgg tgaagtcatc tttttagatg
     7081 gcgtagctta tcaaggaaga tcagatggct cagttgccca attagatgga agtgaactta
     7141 ccccctatgc agcaattaca gattttacgc cagatactag tttttctgtc tcaaaaactg
     7201 cagatggaga acaattgaaa aaagacatat tagtaagaga agccggggaa aacctatttt
     7261 tggcagtgaa gataactgga ttattcaaaa atatgcacat ccgtatcatg ccgaagcaag
     7321 aaaaacctta cagaagacta gcaaaaattt cagagtctca accagaattc caacaaagca
     7381 atgtccaagg aacattagtc gggttcttta cgcctgaatt attccaagga gttgctgcag
     7441 cgggatttca cttgcatttt atcgatgata cacatacatt tggcggacat gtaatggatt
     7501 ttgaagtggc tgaaggaaaa gttgagatta gccgcatctc gtcccttgta cagcattttc
     7561 cagttgatga tccgactttt gttcacgaaa cgattgatta tgctgattta gcagcagaaa
     7621 tccaacaagc agaatagaaa aagacagcga gatgcgttca aggtgattaa cctgacgtat
     7681 ctcgtttttt tgaggaggaa acctagcgtt tacaaaaaaa ctgagctata atcttctcca
     7741 tagatcaaaa aggagcggtt tttatcatgt ggacagaaaa acagaaaaaa gatgcatacg
     7801 aaattttatt attacagcaa caaggattat tagaagcaga agataactgg ttagcaaatt
     7861 tagcaaattc atctgcattg ttgaatgaga ctttgcctga gactgtcttt gcaggatatt
     7921 atttattcga tggtaatgaa ttgattttag gaccgttcca agggcgagtt tcttgcacac
     7981 gtatcaagat gggaaaagga gtttgtggag aatcagctga aaaacaagaa acattgatcg
     8041 ttgatgatgt caaaacccat gcaaattaca tttcctgcga ttcagccgca caatcagaaa
     8101 tcgtagtgcc aatggtaaaa gaaggtaatt taatcggtgt tttagatatc gattgcggag
     8161 taacgaaagg gtatgatgaa atcgatcaag catttctaga aaaatatgta gatcttttat
     8221 tagcaaattt taatataaat gctttttcat ttattaataa ctaaaatcgt ctattgaaac
     8281 aaaaaagaag agaaatcaag aataaaaaga gccggaaaga cagtgggcat ttctgttttt
     8341 cggttttttt atttttttat aaaatcacaa gtgttattga ttttcataat catattttaa
     8401 tggtattatc attttaaata atggaaaaac attaaggatg gatgaaatat gaataacgaa
     8461 actatcattt taggaaaaaa atatatttgc caacctattg gcttgaaaca aaaagtggtt
     8521 ggagaagtaa tcagtaaatt agaaaattgt gttgtgatct gcgtagacga atatcatgac
     8581 gtcgatcgca atgaaatatt agaaaaatgc ggaaaagtag tggcaaaata tactcacgtt
     8641 tatgagttgg aaacaacaga atatctatat aactatagca acaaatatga acatacttta
     8701 gtttcataaa gatttaaata aagtgccttc aaaagcgaga agaccaagct tttgaaggca
     8761 ctttatttag ttggcagtga ttgttcatga tttttgattt attcaaaagg ttcgcctaga
     8821 gaatctgtct ctgtaacgag gcctggtgga gaaactcgat aaaaacctat actgccagaa
     8881 ctgtcctatt ttcggataga caaactagta gttttaaaaa gatcatccga tccaatcatt
     8941 tcaaaaaatg caaaactgat atcaggatca tttagctcag gcttgttatc gtaaagcatt
     9001 tgtaaaggtt gcatttagtt aggagtaaac gcgaaactat catcattatt tgctgaagaa
     9061 gatgaagaag ctaattcaga gtttgtttga aataaaggag acgtgctgac agtttcttta
     9121 ccctgttttt tgcagaggta gaaactttta tatcggaagt ttctaccgga gaatttttct
     9181 catttgtaga aattgttgaa tcgatactat tcgaaatttg cgtatctgaa ggtatgttct
     9241 ttgatgcaga agtcccacaa gcagtaagta acaataaatt tgataaactg ataaacagaa
     9301 ttcttttcat tatgtacaca ctccttatag tatcgtttag catacaccta tgctcagtat
     9361 taggcaaaat caatgcaaaa ctatcaagtc ttttatagta tctattagtc ttagtaatac
     9421 atatatggag agggtgatat cccagttcaa accagataca ttggttacca tctgtattaa
     9481 cgattatgcc tctaaacgct ttttttatga aaaaatttat aagtgaaaac gtaggtatga
     9541 tattatctag aggtaatttt tttgggtatt taaatagttg ctaggaggat ag
//
LOCUS       DAZKMB010000107         9570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000107 DAZKMB010000000
VERSION     DAZKMB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.108"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..818
                     /locus_tag="ACG8V3_002297"
     CDS             <1..818
                     /locus_tag="ACG8V3_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0300779.1"
                     /translation="LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYL
                     ECYFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHR
                     VKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIVD
                     RLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTLRTFKKFLP
                     QIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTFLTK"
     gene            938..1138
                     /locus_tag="ACG8V3_002298"
     CDS             938..1138
                     /locus_tag="ACG8V3_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0300780.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1214..1411
                     /locus_tag="ACG8V3_002299"
     CDS             1214..1411
                     /locus_tag="ACG8V3_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300781.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1708..3255)
                     /locus_tag="ACG8V3_002300"
     CDS             complement(1708..3255)
                     /locus_tag="ACG8V3_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0300782.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3339..4142)
                     /locus_tag="ACG8V3_002301"
     CDS             complement(3339..4142)
                     /locus_tag="ACG8V3_002301"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0300783.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="ACG8V3_002302"
     CDS             complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="ACG8V3_002302"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0300784.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="ACG8V3_002303"
     CDS             complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="ACG8V3_002303"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0300785.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="ACG8V3_002304"
     CDS             complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="ACG8V3_002304"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300786.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6527..7456)
                     /locus_tag="ACG8V3_002305"
     CDS             complement(6527..7456)
                     /locus_tag="ACG8V3_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0300787.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7651..8334
                     /locus_tag="ACG8V3_002306"
     CDS             7651..8334
                     /locus_tag="ACG8V3_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFQ0300788.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8495..9538
                     /locus_tag="ACG8V3_002307"
     CDS             8495..9538
                     /locus_tag="ACG8V3_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0300789.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2931 a         1956 c         1900 g         2783 t
ORIGIN      
        1 ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa
       61 tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc gaaaatcgac
      121 gcttgcctta tttagaatgc tatttttctc ggttttcatt agcgactcgt gaagccgttc
      181 aatggattgt tattgatatg tatgcgcctt atgtttcatt ggtgaaaaag cttttcccga
      241 atgctcagtt gatcatcgac cgtttccaca tcgtccaaca tattggcaga accttcttga
      301 atcatcgagt gaaagaaacc acggcacggc ttaaaggaac ttcccactct caacgagggt
      361 tagggaaaaa attgaaacgc tattggaaac tcctacaaaa agaagaagca aagttaaact
      421 acgaaaaaag ggtttggcgt gccagtttta aagattattt gactgaaagt gagattgtcg
      481 atcgactgtt atctgcttgt cccaacctgc gccaaggcta tcagttatac caagacattc
      541 tttatgcggt aaagaaaaga gatcagtcat tatttgagga ctgtttaaac agagaagtca
      601 gcggtcttcc tgaaacctat acaaccactt tacgaacgtt taaaaagttt ctgccacaga
      661 tccaaaatgc gcttcactac agctattcaa atggaccatt ggaatgttta aacaaccaca
      721 tcaaagtatt aaaacgcaat gcgtacggct ttcgcagctt ttacaatttc aaactacgga
      781 tcatgattcg tcatggcaaa acatttttaa ccaaataaac agaacagaaa gagagtttct
      841 tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacatcat ttgacaaaga
      901 gcctgtattt attactgtcg aggacaatgg catcggcatg attgaagagg aacaaaaaaa
      961 catttgggat cgctattata aagccgatcc gtcaagtaaa aatacaaaga taggcgagtc
     1021 aggtcttgga ttatcaatcg tgaaggaact agtgcgcctg caccacggtt caattgaagt
     1081 gaccagccga aaaaatgaag gaacgacctt cctgatacaa tttccaaaag aaaaataaaa
     1141 ttaccagtaa aaaaccgtgg agagatagct gctattgatt tttgctatgt cttttctttg
     1201 agaggtatcc ggtatggtgg agtgtctgaa aaataaaaaa atggaggcca attttgtgaa
     1261 agctgttatt tctggaagta ttgttttcca agaagaatat cgagatatgg aaaaagattt
     1321 cagtaaaaaa ggcattgaat taattgatta tcctcgctca tgtccgatcc tagacaaaga
     1381 gtatccagaa atcttgacga ctttttttta aatatcgcag cagcggattt attttttgta
     1441 tttaatcaag atcggaaagg ggtattaggc tatattggtg ccgcaacttt ttcagaaatg
     1501 aagtattgtc tgatgcaaaa tcttattcat ggaaaaagga tcaaaatagt cttatttaag
     1561 gaaccatcag aggaaaattt tttgttttga tgaggtagat cgctggctaa agaatggctg
     1621 gatggaaatc agacagccta acaatttggc ataacaaagg tgtgggcaaa aaactgctca
     1681 cacctttttg aattttttat ttatatacta caaaatcact tgaaaattat gattcacttt
     1741 tgcttcgatg cgtgggtgaa tattcaaata gtcggcaatt aattcggtca tgtcgatttg
     1801 gatttctcgg acaattttct ccgcaccaaa catcgcataa tttcctccgc cgattgcccg
     1861 atattgattt gtcaccactt ccagcatttg ttgcggaaga acggggtgac ctttaaaacg
     1921 aagttcggta atccgttgtc caacaggctg tttcatatca atagtgtact gaatcccttc
     1981 atacatgtca taattgtagt actggggctt aggctgaata aaggctggat tgaatacgat
     2041 ttggttattt tctacagcta aataatttgc ggtctgctcc aacgccgctc tcaaatctgc
     2101 accatttatt tgaatcactg ccagagtatt cggataaata tagttggtga tgacgtcccg
     2161 catcgtgatc gtctcaccaa agccttttcc ttcattattg aataacgcag ttccagaaat
     2221 ctctgagccg ctggcaaata attgtacacg attaataaac tctacatagg gatgttcctc
     2281 aagacgagcc tgcatcggat cattaatacg catatttccg ttcactttac ctaagggttg
     2341 atccaaccat ttctctagtt tttcttctac cggtacgatc gctgcaagaa tttctggatc
     2401 aggctctgat ttttcaactg agtgtaaagc aacctggtga tctgtaacaa ccacttgccc
     2461 ctcttttttc tcgacggtaa gacgaatttc accgatgaac tcgccacgat agcctggttg
     2521 aacaacggga actccatgaa ccgtttcagc aattttccgg tgctgatgac cggtgaaaag
     2581 ggcgtcaatg ccttcaacct gctgcaatag ctcgtagcct tcattctctc cagttagcgc
     2641 ttctgtgtcc tcaccggtta acaagtcttt ttcaaatccg ccgtggtaac taacgattac
     2701 caagtctgct tgtttcctta atagaggaac atattctttt gctgtttcga cgatactctt
     2761 aaacgtaatt cctgctaagg tttctggctt ttcccagtga ggaatgtatt gtgtcgttaa
     2821 tcccaagata gcaattttta cacccacctt ttcgataatc ttataggcgt ttccaaagta
     2881 tggttggtca tttttgttca ccatattggc tcctaatata ggcgcttgat aattcttaat
     2941 cgcttcctcg agataatcca caccatagtt aaactcatga tttcccaaca cttgaacatc
     3001 atagctcatt tggttaatca agtctgtaag ctctttggct gaaccatatt ctttagcaag
     3061 ataataactc aaaggagacc cttggatgaa atcaccattt tcgatcgtaa ttaccggtcc
     3121 atctgctttt gcccgtaatt ccttcagttt cgacgatact ttagccgttc ctaaggccag
     3181 atcggttctg ccggaatagt ctgttggtga aacatagcca tgcatatcac ttgtctctaa
     3241 aaacgtaatg ttcattctgc gtgctccttt agctgactca aaatagaaaa accgaagcgt
     3301 attcagctgt tcggtctctt tttttgattt ataatgcctt aatccttcat ttgaagtgtt
     3361 tttaaaatgt tgcctggaat aaagttttct gttgtctggc gaacaatcct tccttgatcg
     3421 agatgataaa ggttataccc atgacgttct tcaaagcgta cgaagccatc gccttcatct
     3481 tcaccaacaa aggacatact cgatacagta aaatagggga tcccattaat ttcatcagta
     3541 aatgtgtgat gggtatgacc gtttaggata cagcgaatat cgtaaggtgc gattagcttc
     3601 aagaaacgtt cggtaccagg ccattttggt atactgcttt ggtgagacag tagatgatga
     3661 tgggtgacca agaaaatcgg ttggtctttt tcttgctcta aggctttttt cagccattga
     3721 aattgctgat cgtccactat accatctgcg tatccatagc ttgaattatc aaaggaaatc
     3781 actgtaaact caggatattt gactatctgg ttgtacgggg cagaagaggc ggcttcatcg
     3841 caccagccta cccaaaaatg ttctttgata tcgtgattgc ctagagtaac aagaatcgtc
     3901 gtttctccta gcgcattttc cagccaagtc ttcaagtatc gataatcgtc tatttcgcca
     3961 tcttcggtta aatctccgct gataatcaca aggtccaagg gtgtttgctg caggagatga
     4021 tgaatgcatt gctccagagg gataagggga ttctgcatcg tagccagcat tcccttatag
     4081 ccttcttcac aagcctcata ctctctgcga aagtgtatat ctgatatatg caatatattc
     4141 attcttatct caatctcttt cttataattc cggttatttg atctaaaata aacacggtta
     4201 cgactacgcc aatcaaaata atactcactt tgctccaaga ccgagtttga atcgcgaata
     4261 tcaatggagc accgattccg ccagcgccaa ctaagcctaa ggtagccgca ctacgaaccg
     4321 ctatttcaaa atgatttaat gctaaagaac aataaatcgg catcaaatag gggatgcgaa
     4381 caaagaaaat cgtttgccaa aaatttgctc ctaccgcatg catttcttca acaagtcgct
     4441 catccatcgc ttccatttct tctgtaaaaa gtttgccaag cattccgatc tgatggacgc
     4501 caatcgccat aacaccagca aaggggccag gtccgacgac tttcacaaaa ataattgcaa
     4561 aaactagttc aggaaaagct cgtaaaacat tacagataag cttgccaatt tttgttaccc
     4621 atggatgtgc ttgccagagg ttaaccgcac tgatcaaggt aatgggaatc gctaataccg
     4681 tagcgatcat tgtccctaaa caggcgattc caatggttaa taatagcaaa ctaatcaaat
     4741 cctcgccgct gccatcatat agaaactgcc aatcaggctg acctaaccct ctcaaaacgt
     4801 ccattcccat ggaaggagag aaggtgctta atccagagta atccagagta gcggccgaca
     4861 aaataacgag aatcactaaa gcaactaatg ataaaagggt tcttctgttc atttcacgaa
     4921 gcatcataac agggacctcc tgacacgttc acttaattta tcaacgatcg cgacagttac
     4981 taggatcgtt agaataatga ttgaaactcg ttcataattc aaaagcgcaa gtgaggaatt
     5041 tagaataacg ccaataccac cagctccgac atatccaagg acagtagaag cacgaacatt
     5101 cacctcaaac gcgtaaaaag catagctcgc aaactgactc aaaacttgtg gagcgatcga
     5161 ccaaatagcg atttgcattt tatttgctcc aacagccgca gcggcttcaa tcgggccaaa
     5221 gtcaatggtt tcaattgctt gaaacattaa ttgtgacacc ataccaaaag taaagacagc
     5281 gatagtcaac acgccagtcg cttcaccaat tccaaacaca gcaacaaaaa gcgcggctaa
     5341 aagcagggta gggatggtcc gtactaaacc taaaagaaaa cgaaagatgg tagaaagaag
     5401 acggttttct gtcactacag tggtcgctaa aaaactaaca gggatcgcta aaattaagcc
     5461 aatgaccgtt ccaagcaaag acatttgaag tgttttcacc ataggctcga ctaatttagg
     5521 taaatagcta aagtctggat gaatgaagcg tgataaaaag acagtcattt gattggagtt
     5581 ttgaaaaacc tgagaaaaat cagctgacgt gatccaggca cttccttgaa aacaaacaat
     5641 taagaacaga agaataagca ggtgtttata ccatttaaag tgaacggttt ctttcaagct
     5701 catcgatttc gcctgccttt tcttcaaaga ttgcattgcc ataaatttgc tctaagcgtt
     5761 catcggttaa gtcgtctgga cgaccatcaa agacaacctg agcagctttt aacgcgagca
     5821 cacgcgtaga aaactcacga gccagaggga ctgaatgcag attgacaatg actgttttat
     5881 ccatcgtttg attgatattt tttaggtcca tcataacctt ttgagtcgtg attggatcta
     5941 atgatgcaac gggttcatcc gctaaaataa tgggggcttg ctgcacgagt gctcgcgcaa
     6001 tggatactcg ctgttgttgc ccaccactga gctcatcact gcgtgtctgc aatttatcag
     6061 acaaaccaac tttttccagt gcttcctttg tctgctggta gtcttccttc gtaaaaagac
     6121 ctaaaatact tttccaagtg gagtaatagc ccaaacgtcc agatagcaca tttttctgaa
     6181 ccgtactacg ctttaccaga ttgaagcttt gcgagattag accaatctgg cggcgaatct
     6241 gtcgcagctc ttttttattc gcttttgtaa tagaggttcc attaatgcga atatctcctt
     6301 tggtggtttc tgccaagcga ttgatactgc gcagcagagt gctctttcca gcaccactca
     6361 accctatgat ggaaacaaat tctccatctt caattgttaa gtcaatatcc ttcaagccaa
     6421 caacgccatt gggatagacc ttcgaaacat tgtcaaaaat aatcattatc cttctccatt
     6481 tttttaagtg taagcaaaaa aaagcttgct gacacttact gatgttttat tctgctgctt
     6541 ttttcgtgta gtctctgatc gtgtcatagg cagaatcagc agcttcttca tagccctgat
     6601 gcccccacaa agccattgct tctttgccat ctttatcatc cttcatattt aagaaggtct
     6661 ctttaatttt cgcttttaaa tcttttgaca tgtcaggctg tacagcgatt gcgtcattcg
     6721 gaatatcgcc ttttgtcagg tacaggactt ttaaatcctt aaataaatca ttatctggga
     6781 aattcgaagc aaaaacattt cgcgcgcctt caaaaacaaa cgccgcatcc atttgcttgt
     6841 tcaacactgc ggtaatctca ctcggaatat cattcacagt ggtcatcttt gtatctgcag
     6901 taggatcaat cccagcatct ttcaattcag cgacaggata gatgtagccg ctagcagagt
     6961 taggacttaa ggttgcgata ttttttcctt ttaaatcagt taacttattc aatccgctat
     7021 cggcacgaac caatacttct cctttaaagg tgttcgtaag tttgtccttt aatggcaagc
     7081 cagtttcttg atcataatcg ccgagctgag aagtcaggat tgcatctgca gctccagcat
     7141 cctttgcttg aacataggct gcaggcggca taattccgac atcgacttga ccagaagaca
     7201 tcgcttcgac gatcgttgaa taatcagtag ctaacgtcac ttctacctca cgatctaact
     7261 ttttcgataa atacttagca aatggttttg cttttgcctc catactacca tcattgtttg
     7321 ttggaacgaa ttgaacaact aatttttctt tgctctcttt ttctgagctg ctacttgtat
     7381 tcccagaacc gcaggccgcc aaagtggtca ataacatgac tccgatgaac ccttttgcta
     7441 actgtttgaa ctgcattttt ctcctcctgt tggatataat ttctaatacg tctaaatcat
     7501 agcagggaat caaaatcaat gaagtgacgc acgtcctatt ttacacaact tttacataca
     7561 atttacaaac aattataaat aattattcgt attgagccga aaaacaatct aagctataat
     7621 aaagaaaaac ttcttagagg tgacgatttc atgaaaaaaa tccatgatac gaagcaatta
     7681 actgcttacg ttgagaagca caacctgcag gattatatgg attcagatat attggcaatg
     7741 gcttctttac ttttttttga aaaggaagag cacctcattc aaacgggaaa aacctcagat
     7801 tatttgtatt ttttagtgga tgggacggta atggtgtact cgtattcttc tgatacacaa
     7861 aatatttgca tcgattacgc aaagcccgct acaccactcg gcgaagcctc ttcattgtgg
     7921 gaattatcgc cgaaaagcag cgttaaagca gtgactaagt gcatctgtat cagcattccg
     7981 ctaaatcaat atcgaacaca gcttcaaaaa gatgttcgct ttttgcagaa tatttgccag
     8041 ttactaagtt atcgtttaaa ttccgggata aatttggcga actcactgac agaacctgtt
     8101 gaaactcgtt tagcaaagtt tattttgacc catcacgaaa acgaacgttt ctcatttcgg
     8161 ttaaccacct gcgcctcaat cttaaatgtc agttatcgac atcttttacg aacaatcacg
     8221 aattttcgtg aggagaatat tatcgaaaag aaaaagaatt attacttgat tcatgatatc
     8281 gaagctttag aggacttagc tgagaatata agtagtccag taaaagcaga ataacagcta
     8341 taaaaaaaca gcagtcagaa gtgattgctg gttttttgat agtggagaaa ttgggaaacg
     8401 gaaaatcttc gttgaagagt agtgttctat atattggtat gctatacttt taaaagttca
     8461 atgtgtcaag acacataaaa aggggagaaa aattttgaag aaaatacttg tttcattgtt
     8521 gtttttggtg tcattagtaa cgttagtcgg ctgtagtcag tctcaggggg agaagcaaag
     8581 tgaaaaagag aaaagtaaaa tcgctttggt tcttggattt ggcggggttg atgaccgctc
     8641 cttcaatcaa gctggctggg aaggattaga ggcttgggga aaagagaaaa agctgacagc
     8701 aaaaaaagat tttacgtata tagaaacgaa caatccagag gataacagta ccaatatcga
     8761 tcaggcaatc agaaacggct ttaccaccat tattagcttg agctacttgc aagcagagga
     8821 actggaaaat gctgctaaag ataatccaaa gttgaaattt gccttggtag atggtgagat
     8881 tactgggaaa aagaatatct catcagcaac ctttaaagat caagaaagtg cgtatttagc
     8941 aggactagct gccgcatata cgacaaaaag tgatcaagta ggctttattg gtggaatgga
     9001 agggcccgtg atccaacgtt tccaaagcgg atttgaagct ggcgttaagg caggggccac
     9061 agcactgaac aaggaaatta ctgttaacgt acaatatgct gcatcctatg attcgctaga
     9121 taaaggaaaa gctattgctg cttctatgta caaagaggga gtcgatattc tttatcaggc
     9181 ggcaggaagt actggagctg gggtgttcca agaagcaaaa gcccgcaatg aaaacgacta
     9241 tagcagaaaa gtatgggtga ttggtgtaga tcgcgatcaa gcggatgaag gggattataa
     9301 aatggagaac ggcgagacag ggaatttcac gttgacttct acaaagaaaa atgtcggtgc
     9361 agaagtcaaa gaatttgcag aaacttctca taacgataaa tttcctggcg gcaagcattt
     9421 gacctttggt ttgaaagaag aaggggtaga tctgacaaaa ggacaattat cagaggaagc
     9481 ctatcaggca gtttcagacg caaaagagaa aatcatcagt ggtgatttca gactgtagac
     9541 aaagtccatt ttatggatga tgtctacagt
//
LOCUS       DAZKMB010000108         9557 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000108 DAZKMB010000000
VERSION     DAZKMB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.109"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(223..1005)
                     /locus_tag="ACG8V3_002308"
     CDS             complement(223..1005)
                     /locus_tag="ACG8V3_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300790.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1070..1906)
                     /locus_tag="ACG8V3_002309"
     CDS             complement(1070..1906)
                     /locus_tag="ACG8V3_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0300791.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1924..3333)
                     /locus_tag="ACG8V3_002310"
     CDS             complement(1924..3333)
                     /locus_tag="ACG8V3_002310"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0300792.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3347..5170)
                     /locus_tag="ACG8V3_002311"
     CDS             complement(3347..5170)
                     /locus_tag="ACG8V3_002311"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0300793.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6013..6339)
                     /locus_tag="ACG8V3_002312"
     CDS             complement(6013..6339)
                     /locus_tag="ACG8V3_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300794.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6385..6747)
                     /locus_tag="ACG8V3_002313"
     CDS             complement(6385..6747)
                     /locus_tag="ACG8V3_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0300795.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6784..6998)
                     /locus_tag="ACG8V3_002314"
                     /pseudo
     CDS             complement(6784..6998)
                     /locus_tag="ACG8V3_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7126..7461
                     /locus_tag="ACG8V3_002315"
     CDS             7126..7461
                     /locus_tag="ACG8V3_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0300796.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7537..9534)
                     /locus_tag="ACG8V3_002316"
     CDS             complement(7537..9534)
                     /locus_tag="ACG8V3_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300797.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         2944 a         2004 c         1431 g         3178 t
ORIGIN      
        1 aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt
       61 aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga
      121 aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata
      181 acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt
      241 aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatctttttt
      301 cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca ctgcattact
      361 gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt
      421 atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag gtggtatagc
      481 tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag tatctacttc
      541 tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg taactgcttc
      601 tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt gtccatcttt
      661 agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac cagttctttc
      721 aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga catgtaaaag
      781 cattttgttg tttacatcta cataataatc ctttgactct acttgagacc ttcctatacg
      841 gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa tctgccaatc
      901 tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca cttcactggt
      961 agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat caggagcctt
     1021 agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt cagtacaccc
     1081 ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca cccatctctt
     1141 cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa cgctgcttca
     1201 ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc attcgggcaa
     1261 taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta tcaacctttt
     1321 tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt accgagagca
     1381 tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac gcaatgtttc
     1441 cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg atagcatagc
     1501 agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg cgattttgaa
     1561 aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa
     1621 atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata attcgcttga
     1681 cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc ttttttagag
     1741 catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga accaaatcgc
     1801 ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg ccttgcttat
     1861 ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga ttcaccgttc
     1921 tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc tttctttttt
     1981 tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa aaccataacg
     2041 cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta aataaccaaa
     2101 aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg ctagataatc
     2161 aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc
     2221 attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca atgttaatcg
     2281 tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag gattttcagc
     2341 tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc tagcggaaac
     2401 atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat taatcgcaat
     2461 aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt taggatataa
     2521 tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa ctgctacatc
     2581 taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat cttctggact
     2641 ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc ctttaagatt
     2701 caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt gattaatttg
     2761 aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg ctccaggatg
     2821 aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt caccatactc
     2881 agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat ttaaccaacc
     2941 gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa tcggttcgat
     3001 tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct tttgattggt
     3061 tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa
     3121 gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat ccgaggcaac
     3181 ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg gttgtttccc
     3241 atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc ataaaaactt
     3301 ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa ctaatttgca
     3361 tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg taattttcac
     3421 tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg gcagcgaaat
     3481 cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca aatacttcaa
     3541 agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat tcaatacctt
     3601 ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact tctccctcaa
     3661 caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc aatagtccct
     3721 tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt accggatttt
     3781 caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta tcgtcaaatc
     3841 caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta ataattgcgt
     3901 gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag ccaggggaaa
     3961 caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt gcaccaccaa
     4021 tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt gaaggctcag
     4081 taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac tctttgtttt
     4141 tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa gccagcattc
     4201 ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg attggcgtca
     4261 ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc aaaaatggaa
     4321 ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga tcgttcagca
     4381 acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa aataccagca
     4441 tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag ggctctacat
     4501 aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa tctgcagcta
     4561 ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt gaaatatttg
     4621 gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca aactttatgg
     4681 ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca atagtattca
     4741 aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag acggccaaaa
     4801 tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag gtggtagaaa
     4861 tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg ccctttttaa
     4921 ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca ttgccaatca
     4981 ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt ttttctaagg
     5041 ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg gcacaatggg
     5101 ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt cctacttctc
     5161 tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca
     5221 atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa catgccaata
     5281 acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa cagggaattt
     5341 aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc acatcaacta
     5401 gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac tataccagta
     5461 aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat
     5521 ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga atttggtgag
     5581 taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata cttgtttttt
     5641 tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa ggtatcgata
     5701 gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact gatttatttc
     5761 taatctacat gtagtaagta tttcaattcc accaattatc aataaaaata ccaaacatat
     5821 ccataataat tttttctttt tcatttcttt tatattgtta taacagtgaa taacataaaa
     5881 attcccgaca atcattgtaa tatttttttg tcacctgtat tagtatttaa tttataggaa
     5941 actataaaaa ctccctagtg aatttacacc tttgggagtt cctgtttgga cggtatgcat
     6001 aatacattta aatcaattta acattaacac ctcagcatat ggtatctcca catatatacg
     6061 gtctttcttt ggatagtact caatatttat gttgatagca cttccattgt ctaatatagc
     6121 tttagcccgg tatgtgttat ctccattttc atgagtaatc gtcacatcat cacatgatcg
     6181 atttaagttg tactgttctt gtaaaatctg tgttaccaat ggtttcgatg acttctctaa
     6241 tccagatgta cctgtcattt ttgaaaaaat gatacctata atagccaaga caacgataac
     6301 tataatgata aacttcttgt tcatatttac tttattcaac tttttatctc ctatctccaa
     6361 aataatttgc tcttatccct cttatcagta cattgacatc cctaaaagta caccaaagat
     6421 tgctccagct acccctgcta tcatcaaaat cgtacccatt tgttcatctt tatctcgata
     6481 taagtagccc ataataacag caacagcacc aaatacaaca ggaataaatg ctaacgctac
     6541 taccatagaa atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat
     6601 ttgttccttc atttcattaa ttgtctgctc agtacttttc tttactgcgt ctgctccagt
     6661 tgtctcatct gtttcttgta gtgctccgca aaactggcaa tacttacttt catctgttat
     6721 ttctttgcca cattttctac aatacatgtc ttctcctcct aaaaacattc aattgtttat
     6781 tagttaactg tttactttat taaatacatc agaaaacagc tcattcattg gtacacttaa
     6841 tatctcagaa agcatcttca tttgataggg tttaggaatc gttctccctg tttgccacga
     6901 acttacacat tgctgtgtaa cattcatttt tttgctaact ccgtttgaga aagtcctttt
     6961 tcttttctgt atcggctgag attacttact tttttcatga ttctcccctc ctgttttatt
     7021 atatacaaca atatgatgta tttcaactca taaaatataa aaaaacttca aataccatta
     7081 caaatacaac aacttgttgt actatactga gatgggggtg tattaatgaa acagaaacaa
     7141 tatgcaaagg tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa agaattagct
     7201 gaaaaaatct tctctacaca acaggcagta gcaagatggg aaaattctgt aacagagcct
     7261 aacctcgata gcttaaccgc tctttctaga gccctaggaa cccctgtaag ccattttcta
     7321 gacaatgttg tcgtagatta tgaggaagag tttttggctc tctatcgttc tcttagcact
     7381 gaagatgcag ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc
     7441 aatcagctat tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt
     7501 gagatgaaat aagattaaga gctatggata acctgtctag tttaatacaa ttgggtcttt
     7561 tctcttcctt actctccgtc catcatctct tgttacactt gtaaattgat aaagaggaac
     7621 taattcaact cctgataata tcaattcttt tattacttca tctatccctg ctccttttgc
     7681 taggtagact aaaaaactac tacttgcttt cacattgtat agtctcttaa tatccttaga
     7741 agctcgttca agagtgaatc cttgtttaac aaagtagcct ctgttttctt ttaaagcatt
     7801 ttccacgctg ttattcccaa cattacgtag acgttcacaa acagtgttag taagttcttt
     7861 ctctgtcatt agatatgttc cttgccacga taagccctgt aaaacttcat gtgcttcatc
     7921 taaaatcaat tgtttttcct tgctaagttc tgatcgggaa ttttgaggaa atactctttt
     7981 agcctctttt tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa
     8041 atgaaatttt tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc
     8101 attaaaaacg tctaatcgat agacattgct tctcttatga ttaaagtttc ctaacgcttt
     8161 ctcttccttt aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa
     8221 tttttttatc aatcctataa aagtcataag tgatagtaac ttattacatt tatctatgtc
     8281 ataagtttta ccatagtaaa aactcatttt tttagctaat tgttcccccg acaaaataca
     8341 agtcacactt tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac
     8401 acgtcgcatt tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc
     8461 aagagtgtca aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa
     8521 gtctaggttc tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag
     8581 catcacaact ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat
     8641 ggaatgccca gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag
     8701 acatcttaag tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc
     8761 ataaatctca ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt
     8821 catttcttct ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc
     8881 atactctctc cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg
     8941 aagctcagta atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata
     9001 tcctccaaaa aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt
     9061 tttcaagtat tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag
     9121 aaagacaatc ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa
     9181 cgcagaatta tgaactaaat tgatagataa aaccttttct atgcttaagt atctactatc
     9241 atctttactc aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag
     9301 aataattact ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct
     9361 tggaataaat gattgtgatg aatttagtat ctctacaaac tcatctcttg taacttctct
     9421 atagttatct aaacacctat ctcttacatc agttttttct ttttttgtaa ctggcttctc
     9481 taataatttt tctttatcta cataaattgg aatattaatc attgatgttt ccatttgtgt
     9541 ttcctcctta taaaatt
//
LOCUS       DAZKMB010000109         9557 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000109 DAZKMB010000000
VERSION     DAZKMB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.110"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(437..1051)
                     /locus_tag="ACG8V3_002317"
     CDS             complement(437..1051)
                     /locus_tag="ACG8V3_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0300798.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1657..2034)
                     /locus_tag="ACG8V3_002318"
     CDS             complement(1657..2034)
                     /locus_tag="ACG8V3_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300799.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2334..3215)
                     /gene="galU"
                     /locus_tag="ACG8V3_002319"
     CDS             complement(2334..3215)
                     /gene="galU"
                     /locus_tag="ACG8V3_002319"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0300800.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3258..4277)
                     /locus_tag="ACG8V3_002320"
     CDS             complement(3258..4277)
                     /locus_tag="ACG8V3_002320"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0300801.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4910..5914)
                     /locus_tag="ACG8V3_002321"
     CDS             complement(4910..5914)
                     /locus_tag="ACG8V3_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0300802.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5927..6976)
                     /locus_tag="ACG8V3_002322"
     CDS             complement(5927..6976)
                     /locus_tag="ACG8V3_002322"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0300803.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6993..7814)
                     /locus_tag="ACG8V3_002323"
     CDS             complement(6993..7814)
                     /locus_tag="ACG8V3_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0300804.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7801..8580)
                     /locus_tag="ACG8V3_002324"
     CDS             complement(7801..8580)
                     /locus_tag="ACG8V3_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0300805.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8597..9067)
                     /locus_tag="ACG8V3_002325"
     CDS             complement(8597..9067)
                     /locus_tag="ACG8V3_002325"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0300806.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(9079..9498)
                     /locus_tag="ACG8V3_002326"
     CDS             complement(9079..9498)
                     /locus_tag="ACG8V3_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0300807.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
BASE COUNT         2816 a         2048 c         1525 g         3168 t
ORIGIN      
        1 tgtgtatttt atttctatta gaaaaaggta tcctgttgga caattttgtt atatcaggta
       61 aacttcattg caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga
      121 agactctttc atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt
      181 ctctttttcc actacttttt atttttctga cactacatat tcttataact catttctcat
      241 aactgtgaaa caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga
      301 tgatccatct taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat
      361 cgttttcttt ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt
      421 gtcaacatgt ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat
      481 acaaaacttg ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat
      541 ctagatttgg caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat
      601 cagcaaattc aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat
      661 caaatccagt aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga
      721 tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag
      781 aaagctgatc aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg
      841 gattttcaat caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta
      901 caccacttag tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg
      961 cgatccaagt gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt
     1021 ggtgataata gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca
     1081 tgaatagaaa cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa
     1141 cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc
     1201 ttgacatagc aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt
     1261 ttcaccctag attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg
     1321 atcggtcagg gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt
     1381 cagggagcct ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc
     1441 caagtgcaag caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca
     1501 taaaaaaagg aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat
     1561 ataagtgaaa aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc
     1621 attattttat ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct
     1681 actttgttcg agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat
     1741 ccgagacttt cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa
     1801 agcaaatacg caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc
     1861 aacggatgct gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa
     1921 catgataaat agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat
     1981 cagccacttg agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa
     2041 cacacacctt tttttataat taaaaaattc gcctttttat tctactatga atcttctggc
     2101 ttggctttca tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac
     2161 cattagcata atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca
     2221 cgactcaact gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc
     2281 tgccgcttta ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt
     2341 tctgttctta gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc
     2401 gaatgttgaa gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg
     2461 tatctttttc ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt
     2521 gcatccgtta gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat
     2581 attttaggag tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt
     2641 tcaggttttt ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga
     2701 tcaataattc cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa
     2761 gcttgtgttt cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc
     2821 aaatcatccc ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc
     2881 acggcatccc ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc
     2941 gtttcttcaa ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat
     3001 tcgatatttg catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag
     3061 atgtcttcga ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc
     3121 acgattggaa gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc
     3181 ccagcggcag gaatgactgc ttttttgacc ttcacttgct gttcctccta aacgacgatt
     3241 aactattgat catctattta acgaagagaa aattcattct ccatttttcc gtcacgtaac
     3301 atgatttctt ttgctgcttt tttgatatct tctccattat acaaaacatt atagattgtt
     3361 tgagtaattg gcatctcaac acctagctgt tgagctagct ctactgctgc ttttgtcgtt
     3421 gccacaccct caacgaccat tcccatgttt tctagaactt cttccagctt atgtccctgt
     3481 cctaacaagt taccggcacg ccaattgcga gaatgaacac ttgtacaagt aacgatcaag
     3541 tcccccacac cactcaaacc gataaatgtc agaggattgg cacccatcgc gacaccaaga
     3601 cggctgattt ctgccaatcc tcgagtcatg attgctgctt ttgcatcatc tccgaatcct
     3661 aatccatgga tagcgccggc accaattgca atgatatttt tcagtgctgc accagtttct
     3721 acgccaatca catcattatt tgtataaatc ctgaaataat cattcatgaa cagtttttgt
     3781 acataggctg ctgctttttg atcaatactt gcagctgtaa ttgttgtgat atcatgtact
     3841 gccacttcct cagcatggct aggaccagat aatacaacaa ttgcctgtct tttttcttct
     3901 ggaatttctt cagccaaaac ttctgagatt cgtttatgcg tatcttgttc tagtccttta
     3961 cttgcatgta taatgattgg cttagtattc attttaccaa taagttcctg tgcgactgaa
     4021 cgaatcgctt ttgttggaac aacaaataaa atagcatctg cgttatccac tgcttctgcc
     4081 aattctttat atccgataat cgattctggg atcgtcaaat caggtaaaaa atgctgattt
     4141 gtatgatact tattgatttc atcaatctgt tcaggtacat ttccccaaat acggacatca
     4201 tgcccgtttt ctgccaatgt ttgtgctaaa gcagtgcccc aagatccagg accaagaact
     4261 gcgatttttt gtttcatgga tattcctcca aataaataat tgataatttc cattatagta
     4321 tacatttctt aaagttggga ctaatttcca ttgtttttaa gtgtttgttt acttctttta
     4381 tcgtttttac ttgaaataat caatgatatg ttacaatagt ctacatttta tttttacact
     4441 cgttatgctt tgcttgttcc taagaggaag cagttttgac aatgttacta ccagcattac
     4501 tattaggagg ttaggaaact tctcctagtg agtattgatt taaaatacat aagagacaga
     4561 ttgaacattt ctaaatctgt caggtcctgc aacggacaga ttgtctctgt tgacggttga
     4621 cataggaact tttgttttag tcaacattct ggatacaaaa cgctatgtaa actggcgcaa
     4681 ctcgttgtac ttggactttt actacggatc catgcaacaa ctaaatagta ctattgcata
     4741 acaaaaaatg gttggcaccc acatgtccca ttaaacagat tataagttga actgtagata
     4801 atgcaaaaaa tgtgttttat aacgtaattg atgccaatat tttaaaaaaa taaagggagc
     4861 agttatcgct gctctcttta ttttttatat tctttttatc cagtctatcc taataatcaa
     4921 actgacgata gtagcggcgt atctctaatg gatggcagtt caactgctcc acatgagcat
     4981 cgatgcgttg tgtcactaca tgcatcaata agaaagacaa aacacgtccg cgatattttt
     5041 cactgatacc ttctactgga tagtcttttg tgtctatcaa tgtataattg ctgcaaattt
     5101 ttggcaaaag tttagccaca cgttcagata acactctctg ttcatcttcg cctaaaaata
     5161 atgtgactgg ggttgtttct tctacgatct ctaacgtccc gtgaagaaac tcggcagcat
     5221 ggatagattt cgatggaagc cagctttgtt cttcccagta acacatggca tatgaataaa
     5281 cagctcccca ttgattccct gctccaataa aataatgcat actatcacgt cgatgttttt
     5341 ctgcaaatga taagccaaat gcatctgctt ttttctctgc ttccactaac cctgttggta
     5401 aataacgttc gagctgttca tagtattctg aataagcttc gaattctcca ttcaaataca
     5461 tcaatcgatc tgctgccata aaaaatttga tttgttctgt tccaggagct ggataagtga
     5521 ccacaaaatc gcatagctga gacaacttac tgttcgcttt atcgataaat ccaatcagtg
     5581 tcgctcctac tttcttgatt tgttctactg ctttcactac ttcttgagtt gtaccggtaa
     5641 cagaggacaa aataacgata gaatcttttg ttaatctttt gtttccagta gtgtaatatt
     5701 ctgcagcatg ctgtacaaag acaggcaagt tgcttttccc attcatataa gtgacagctt
     5761 gcatcgagga agcatacgtt ccaccgattc ctagataata gacagcgtca aatttttccg
     5821 cataaagttt atcgatgatt tcttctactt gcggacgtaa agctagtgcg ccttttattg
     5881 ctttgatttg ttcttcttca ttgaatttca gcatgatttc cacctcttat aatttacttt
     5941 tcaagactgt atcaaagtct tcaaaaatct ttttgttcaa gtcaatacct ttagctactg
     6001 cggttttata agcgaatagt tgaaacggaa cgacaagcgc taatacagac agatattcat
     6061 cacattctat tgctaatcca agtgttttct cgtttcttgc tttttttgtg gtaatggtga
     6121 gagtctctcc aacgtaccga ctcatatatc tttgcaactt ttgcgaacgt tccttattta
     6181 cactttcccc ttcaataaag aatagcaaat gcgaagcatc tgcctccagg tatgggccgt
     6241 gcatataagc ttctaactca aatccttgtg acggaacgcg gattgtttca gtcaatttcg
     6301 tctctgcttc tttagctgtt ccccagtttg gaccatatcc aatggtgacg aaacgctgtg
     6361 ctaaacgaaa taaactttga tatccatcaa aaaattcttc cgttttttga ataatcgctg
     6421 gtaaatggtt gattattttg cgcaactgtt gcatgtagtc ttgttctatt tttttgctga
     6481 ttttattttt acttattcca attcctatcc ctaagagtag taactgtaaa acagtcgcag
     6541 aatagccttt tgtaacgaag cctactgatt ctaccccggc acctaaatca ataatttgtg
     6601 cagcttctaa agcaagtgga ctttgcacgt catttgtgat agcaacaaca ggtaaccctt
     6661 gtttttgtat catttttact gcttcaacag tagaagcact tttcccgctt tgtgacacag
     6721 caatcacagt atcgattgaa ggagaaagtt ttccataatt gaaaaaataa aaaggttctt
     6781 gaatatcgac agtaagatca ccataagatt ccaatgctgg cttcgctgct aaacaagcgt
     6841 tataagatga acccgttgct aagatcagta aattcactgt ttttttcata tgttctcttg
     6901 tcgaaaaatc attctttttc aagatcgtac ttagtacttc cttttcttca tagatataat
     6961 cttgcatcgt aaacattttc tctgctcctt ttttaaaaaa tccctatcca cgaaccaaaa
     7021 attccaaaga aagctagtcc gatcaagatc gttaatggtt tcacttcttt cttcaataga
     7081 taaaacacta tgccaaatgc tccaagacat aagatatttg gaacgatatc atcaaaaata
     7141 ctttgaacag tcacagcatt ttctcctgaa ccgattttta aaggaatatt gatggtgatc
     7201 atcgtggcag tcattgctcc aacgaccatc agtccaataa tagacgcgcc taatgtcaaa
     7261 ctttccatca taccgtttgc ctggatcttt ttcaaaaaac ccgttcctaa gcgatatccc
     7321 caacctgttg ctagatagcg aaacagaatg tgcggaatat tgaaaatcaa aatgaacaag
     7381 atagggccta aaatatttcc ttgcatggct aaagaggtac ccacacctgt ggcaatcaat
     7441 cgcaaagttc cccaaaagaa agaatctcct aaccctgcaa gaggtcccat caaagatgtt
     7501 ttgatactat cgatcgcagt cacatcaaat tcaggatcat ccttattttc ttcctccatt
     7561 gcagccgtga ttcctaaaat cagtgtaacg atatgtgggg tcgtgttaaa aaactctaga
     7621 tgccgtttca aagctgctgc catttcttct ttcgactgat ataacttttt caaaatcgga
     7681 atcattgcat aaacataagc catattcatc tgacgttcat aattccaaga aaactccatt
     7741 tgaaaagaac gccaaaatac gtgatttagc tcttttttcg ttattttgct gtttttgatc
     7801 tcagaattct tcatcttctt ctacctcccc tgcagttgtt tgaagtggta gttgacggac
     7861 attcataata ttgactacca caactgctaa aatcgcacca aaaatagcta ctccggtcac
     7921 aggaatctct aagtaagaag ctatagcaaa acctaagaaa aaatatggtg caacttgttt
     7981 gttgatcagc aaacgagcaa gcatcgcaaa accaagagct ggaatcaacc ctgtagctac
     8041 tgacaacccc ttttgaacga agtcaggaat catatttaat agatttccga tcgtattgct
     8101 tcccaccata taagaaacca ttaccacaac accaagcatc aaggaaagac cgataccggc
     8161 aagcaagtgc atatgctcga ctcctttata atttccttct tctgcataat tgtctgcttt
     8221 atggttcata attggaatca aaagccctaa gtaaatattt ttcaatatca atgtcaacgt
     8281 agcaatcggc aaacctagta aaagcgcggt ttctgtaccg gctccagctg taatagcaaa
     8341 agccgtccct aaaatcccac cggtcacaac atcaggtgga atcgaagcac ctactgagaa
     8401 cgatccgata aatgctaact ctaaagttgc gcccataatg atcccagttt ttacatcacc
     8461 taacacgatt cctgtaaaca gccccgtcac aatcggacga gaaagcaacg atgtccctag
     8521 tgcatattct gattgtgcga caaatgcaac aattcctaat aagactgctt gtacaatcat
     8581 tgaggttctt cctttcttac aaaacttctc ttacatccac ttttttatct gctggtactt
     8641 gacggatttc tacttctatt tgtttgtcaa ttaatcgttt caagcgttcc tcctcttctg
     8701 gtaaaatatt taccgctttt ccgatgctgc gagtaccagg ttttgccttg atccctccta
     8761 agttgatggt tttgatttct ggatacgctt ctgccagctt atatgcatcc tcgatcgatt
     8821 ccactacgat gaataactta tatttatctg tcaccccgct tttcaacgcg gcaatcgagt
     8881 catcaattga tttgatcact agtttgactc cttgtggctt ggctaatttg atcgttgtct
     8941 ttcgtatctc attcgtaggt acatcatcat tagcaatcaa aatacagtct gcgcctattg
     9001 tttgtgtcca tgaaaaagct acttgtccgt gtaataaccg atggtcgact cttgtcaata
     9061 caatcatttt tcttcctcct aaaattcgtc ttcttctatt ttctgattga gccgctcgtt
     9121 acaaaactgg atcatttttt ttgaatcagt tagcacctgc tggatcaaaa cagttgtctc
     9181 tacttcagcg tccaaccgag atactagctc aatcaatagt ggcaaattca atccggcaac
     9241 gagatagaca tgatcccgat tgatatattg taagaattcg ttgttgacac ttccgccaaa
     9301 aagatccgtc agaacaatga tttctttatc tttgtacctc ataaaaagct gccttactgt
     9361 ttccgctaaa tcaaaattct catcagtata gcaatcgaat acagtaatag aatgattttt
     9421 cccatagatc agctctaatg aatctaatat ccctgaagca aattttccat gggaagctaa
     9481 gataatttct ctatccactt tttcatctcc tcttttcact tacacatact aaaaaagcaa
     9541 gttgcatgcc aaaatta
//
LOCUS       DAZKMB010000110         9050 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000110 DAZKMB010000000
VERSION     DAZKMB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.111"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..82
                     /locus_tag="ACG8V3_002327"
     tRNA            9..82
                     /locus_tag="ACG8V3_002327"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Ile,seq:cat)
     gene            complement(467..964)
                     /locus_tag="ACG8V3_002328"
     CDS             complement(467..964)
                     /locus_tag="ACG8V3_002328"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0300808.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1004..1813)
                     /locus_tag="ACG8V3_002329"
     CDS             complement(1004..1813)
                     /locus_tag="ACG8V3_002329"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0300809.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2147..3004
                     /locus_tag="ACG8V3_002330"
     CDS             2147..3004
                     /locus_tag="ACG8V3_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0300810.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2997..3899
                     /locus_tag="ACG8V3_002331"
     CDS             2997..3899
                     /locus_tag="ACG8V3_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0300811.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3932..4282
                     /locus_tag="ACG8V3_002332"
     CDS             3932..4282
                     /locus_tag="ACG8V3_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0300812.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4331..5254
                     /locus_tag="ACG8V3_002333"
     CDS             4331..5254
                     /locus_tag="ACG8V3_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0300813.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5332..6225
                     /locus_tag="ACG8V3_002334"
     CDS             5332..6225
                     /locus_tag="ACG8V3_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0300814.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6263..6826
                     /gene="amaP"
                     /locus_tag="ACG8V3_002335"
     CDS             6263..6826
                     /gene="amaP"
                     /locus_tag="ACG8V3_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0300815.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6840..7028
                     /locus_tag="ACG8V3_002336"
     CDS             6840..7028
                     /locus_tag="ACG8V3_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0300816.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7041..7943
                     /locus_tag="ACG8V3_002337"
     CDS             7041..7943
                     /locus_tag="ACG8V3_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0300817.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7975..8217
                     /locus_tag="ACG8V3_002338"
     CDS             7975..8217
                     /locus_tag="ACG8V3_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0300818.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8473..8844)
                     /locus_tag="ACG8V3_002339"
     CDS             complement(8473..8844)
                     /locus_tag="ACG8V3_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300819.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3157 a         1562 c         1748 g         2583 t
ORIGIN      
        1 tttatgatgt acctttagct cagttggtta gagcagacgg ctcataaccg tccggtcgta
       61 ggttcgagtc ctacaaggta cataagaggt tgtgaaaaag ctgccctgca tcaagtaata
      121 agaacagcca agtaaaaaca gcttcttatg tttttacttg gctgttctta ttactgcaga
      181 tgcaagcttt tgaataccgt ttaaggaaga ggttgtgaaa tcagcgtttt gacctgagta
      241 ttagcttaac aaacggaaaa atagttcctc atattttgcc gtttgttaag ctaataccga
      301 gggtcagctg attgaacacc atttaagaaa gaggtcgtga gcctgccgtt tcactctgtg
      361 tcataaaaaa gccaatctac tttgatagaa aaagcacgta ggactaaaac agttctgcgt
      421 gcttttttta ttgtgtgtta aatatcgtaa atgaagatag atatttttat gattcgacga
      481 tagagacgtt gaaatctttc aacagctcta aagcggattc tccgtgtttt ctactggaga
      541 ctaacattgg atcaacaaaa aagtctgctt caggaaaagc actctgcagg cctatcagat
      601 tagagagtac acacaagttt gtttcaatcc cgactacttc gatctctttt tgttcttctt
      661 tgctttcaaa taaagtttcc tggatttcag ctaaagtttc aggtggaatc gtaaagtaat
      721 actttttaat cactcgttca ttttcttgtg gagaaagaag agggatcaat tgcaaatctt
      781 ctcgttcttc ttctttgtcc ttcagttcaa ctggtatatc ccgtgtgaag agaatatatt
      841 cattattttg gcgtgcttta gctaatcttt gagcaatacg ttctgccaat tgatctgact
      901 ctggaatata aaattcgctg tttggatcaa gaatatgatt ttgcatatca ataataacta
      961 acattttctt tcctcctaca ttcatatcgt cattttattc tttttaatag aagtttttta
     1021 aaatataagc aacaccagct tcttcattcg tcagtgttac ttggtttgca atttctttta
     1081 cttgtcgctg tgcattcccc atcgcaatac tcaacccgct gtaattcaac atagcaatgt
     1141 cgttttcttg atcgccaaaa gccatgatgt cactcgattc gattccaagg taatccgcca
     1201 gaaaaacaag tgaatgagct ttagtgatac ctgtaggcgt gatttctaag aaatgcggat
     1261 cagagaatac tgattggata ccatctgctt gcagatctct ttgtagcttc tgtagttttt
     1321 cttcactctt atctgttaat gctaatttaa gatagctaag agaaggatct tccaacatat
     1381 ttaaaatttg atcaaaagaa tggttctgta aattgaaatg ttcataataa gggtcatctt
     1441 tgactagatt tgattcatcg aatgtcagct gatcatcttg ggtttctcgg ctagcaatga
     1501 tggatatttt cgcttcttga gcatagaaga aggcttttct cagtgtatcg ctatctattt
     1561 ttgaacgtct gagaactttc gccatttttc catccttcat ttcagcaatc agtgaaccat
     1621 tggaagaaat caaatagcca ccgattccta attcggaaaa aatatcttta gcagaaatgc
     1681 tgttccttcc ggtagcgacg gttacgattc cgccattttc atcaaatttc cgaagtgctt
     1741 ggatattttc ttctggtatc tttccatttc gatcaagcaa ggtaccgtct aaatcaatcg
     1801 ctgctagttt cattttatct tcctactttc tttttacata tctattttcc tgttacctta
     1861 agattaaatg agaatttaaa aaaatgaaac taacttgctt tgtcattatg aattttttca
     1921 aaaaacattc ttaagaaaaa ggtggctata tatagtagtt tttggtcaat acctttgaat
     1981 aaaaagccat gaaaaacgaa tagttagcgc ttaccgatgc gttttgtgat aaaaaaattt
     2041 taaaatattc aatgaaaaca aagcatatat tttgctttaa aaccaaatga ggaatattct
     2101 agatgtagaa caggttagaa aaagattaga aaaaggagtg attttgatgg agaagagata
     2161 cggcgtagtt cctgtggtaa ctgcttcaga cgaaaattat gctccttact tgagcgtgat
     2221 gatcgcaact gcattagaaa attgcaataa aacaagacgc ataaaatttt atgtgattga
     2281 cgatggtcta tcagaatata gtaaagaagg attggaagaa acggtaaata aatattcaag
     2341 taatgcaagt atccaattcc tgacagtgga aaaagatatc tacgaagact tcctagtcag
     2401 tgaccatatc acaactaccg cttacttaag aatttcttta ccaaacttat tagctaaaga
     2461 ggattataag aaagtccttt acttagattc agatgtgtta gtactagacg atatcgtaaa
     2521 actatatgat gagccgttaa atggaaaaac aatcggcgct attatcgatc caggccaagt
     2581 gaaagctttg gaaagattag gaatcgattc cgatgacttg tacttcaatt caggagtgat
     2641 ggtgattgac attgatcaat ggaataaaaa agaaattaca gaaaaaacta tccattacct
     2701 cagtgaaaat ggcgatcgaa tcatctatca cgaccaagat gctttgaatg ctgtacttta
     2761 tgaagattgg gaacaacttc atccaaaatg gaacatgcag acttcgctga tttttgaacg
     2821 tcatccagca ccaaatgaaa aatatgaacg tcagtataaa gaaggaaatg aaaaaccttc
     2881 gatcgttcac tttacaggac atgataaacc ttggaatacg ttgaaagatc atccgtatac
     2941 caatttatac ttaaaaaaat tagcacacag tactctaacg aaagtaggcg agatcaatga
     3001 ataaaaaaga aatagcagta gtcgcaagtt gtaacacaaa attcgtacca catttagctg
     3061 cattatttgt ctcagtatta gataattgca acccgtctaa atttgttcgg ttctatgtga
     3121 ttgacgatga tatcgatttt gaaagtaaac agcttttacg cttctcagtg aaaaacgcac
     3181 gaatgaactc agatgtggaa ttcttgaaaa tcaataaaga attctttaca aatgtggtaa
     3241 tcagcgatcg tattccagaa acagcttatt atcgtattgc gattccggaa ttgttccgcg
     3301 gcacagaagt tgaacgtatc ttatatatgg attgcgacat gatcgcttta caagatatct
     3361 ctaaattatg gcgtcttgac ttcggtgatt caatcgtagc agctgttgaa gacgctgggt
     3421 tccatcaacg actggaaaaa atgaaaattt cagctaaatc aatgcgttat ttcaactcag
     3481 gtttgatgct gatcaatgtg aaaaaatggt tagatgaaaa tattacgcaa aaagtattag
     3541 atttcatcga acataatcca gaaaaattac gattccatga ccaagatgca ttaaatgcta
     3601 tcttacatga ccgttggtta ccacttcatc cgcgttggaa tgcacaaggc tatatcatgg
     3661 caaaagcaaa aaaacatcca acagcagcag gtgaacgtga atacgaagaa actcgcaaca
     3721 atccttacat tatccatttt tcaggacatg tgaaaccgtg gagcaaggat tttgaagggc
     3781 caacgaagaa atattacgaa aaatatgcag ggatgacggc tttccgttgt gtagctaaat
     3841 tccctaaata tccaaaatat gcaaaagttc aaccaaaaac agatgtagtt gttaaataat
     3901 agaagacaac aactgaagag ggggagtagg aatggaaatt acaatgtcta aaaatgcagt
     3961 agagacgtta attgaaaagg ttggggaaaa tacaaaaatc gcactcgcgc ttatcaatga
     4021 ttccgatcca tttctaaggg acaaaggtgc ttttgcaaaa ggcagctttt ttcaaatcat
     4081 tccttttgtt tcggaatttg gagaatatgc gactaaaatc gagcatccat tattggacat
     4141 ctatacatct aaattagagc aaaattattt cggtaaacga ttgaacatgg acttcaacaa
     4201 acagcttgac agcttttcat tagaaaatga aatcgcagta ctcgattata atatcaaatt
     4261 aaagaactgc ttcttcagct aatagtttcg agaaagtcca aaaaggaggt atacattgag
     4321 atactacttt gtggatgaag aaaagaatgt aatagaaaca gatgaaaaaa aatataattg
     4381 gctcgtactt gatagtgcaa agcccgaaga gattgagcaa gcaatcgaag aattcgagct
     4441 tcctgatgat attttcgtag gaaccgaata tccggaagaa gtttctcgga tggagcgcct
     4501 ttatgagacg aaactagagc atccattttc tttggtagtg attaatctag atcagtcaga
     4561 taatcgtatc gaaaaaaaat taacgccaat atcttttgta ctttccgata atctcttgat
     4621 tacttgtatc gatagaaaaa ccgaatttgt ggatcggctg ctgacaaagc atggaaatca
     4681 tttaaactcg tttgaaaaga tcatcacgta tacattatta gatatttaca cccattacgt
     4741 gaaagaacta agagagatga aaaagcgaat tgacgctttg gataaagcag ctcgtaaaac
     4801 aacggaaaat gaagaactgt ataaacaggc agatcttgaa agagacatcg tatatatcga
     4861 tcatacctta agagatcaac gggaaacaat ggatcaatta tggaattctg aaactttcat
     4921 caaacgttta gacgatgaac agctactcta tgatgtccat ttgagacagc gacaaacaga
     4981 aaaaatgatc gagatctatc gagatttatt agagagtatt ggtgatctat tcaacgggat
     5041 gatggacaat aatctaaatc atatcatgaa atatctagat tcagctgcat taatcatttc
     5101 cgttccggcg ttgttcgcag ggatttgggg aatgaataca ggaggattac cgggtaaaag
     5161 ttctgctcta ggctttatac tcgtagtctt tggctctttg atagcagcag tagttttagg
     5221 ttaccattta taccggaaag attatacaaa ttaaagaatc tatttaaaac aggtatcaaa
     5281 caaagaaatt tgttttggca atacaattta aaaactagga ggaatacagc aatgacagaa
     5341 ccaaaattaa tcgacccgcg aaacttgtac catacggaga aatttcctaa acaagatcag
     5401 gatacaccag ctttacagca gaatatgatt cctgttcctg attgtggaga agaatcctat
     5461 gaaggcaata atcaactaga aaaccgccgt gtactaatca ctggtggaga ttcagggatc
     5521 ggtcgtgcag cagcgattgc atttgctaga gaaggggcag atatcgctct tcaattcttc
     5581 ccaggagaag aaaaagatgc agaggaagta gcaaaatacg tgaaagatgc aggacgtaag
     5641 attgtcttac taccttttga tcttcgtgac gaacaagcac ctaaagaaat catagataaa
     5701 gccgtgagtg aacttggcgg gctagatacg atggtactaa acgcaggaca gcaaatttct
     5761 tgtcagtcta ttgctgagtt accacttgag caagtcaaag acacatttat gataaacatc
     5821 atcgctatgt ttgctttagt gaaagaagct gttccgcata tcccagcagg aggagcaatc
     5881 gtaacaacaa cttctgttca agcattcaat ccaagcgaac atttattaga ttacgcagca
     5941 acaaaagctt caatcgctaa ctttacgatc ggtttagcaa aacagcttgc acctaaagga
     6001 atccgtgtaa atggtgtagc acctggtcca atctggacac cattacaact agacgaagga
     6061 caaccttctg attcgatccc tgaatttggt cagcaaagtt tactagaacg tgcaggtcag
     6121 ccaggagaat tagcaccagt ttatgttttc ttagcatcga ataaagcaag ttatgttaca
     6181 gcccaaattt acggagtaac tggtggagaa gcgatcaact tataagaagt tgatcgtttc
     6241 acccctatga ggaggtgcga aaatgaataa aggtgtaaaa acgatcggag tgatcggaac
     6301 attacttttg ctatctgtgc ttttgtttgc gatactatta agtgcaacct atccattgcc
     6361 ttttcagcta gagaagtttc gattcttttc catcactaat tcatacgtgc aacaatatgt
     6421 attttggctt gccgttgcat ttgctatttt gacaattatc acgttacttg ttttcctgtt
     6481 ttatccaaaa acaatcggaa actttctact gaaacatggt gatggggagt tgacgttgga
     6541 taaaaaagca attgaaggaa tggttcgttc tcacttgcat gaagatgaat tcatccattc
     6601 accaaaagta aatgtcaaag caacgaaaaa caagatcgcc gtcaatgtaa aaggggagtt
     6661 gaaacgaact tcagcactca ttggtaaaac aggaacattg atgaaagaaa tcgaaaatga
     6721 agtacaacac atattgggaa caaaagagcc agtgaaagta tcggtgaaat acacaggctt
     6781 tgaagaacca gctaaacgaa gcaatgaccg caaacgagta gaataggggg ggaccaaaaa
     6841 tgaaagaact attttcgaca tacaagctcc ctatcatctt tggggtactt ggattgatat
     6901 tagccgtttt atggatcacc gtgggctttt tcaagacctt attgatcttg atcatggcag
     6961 cgatcggcgt cgctatcggt ttttatctga aacaaacgaa aatcttagat cactatttta
     7021 aatcttagga ggaaacatcc atgaataaca ataacaaaaa taataacaat aaaatgaaca
     7081 accaaaaacc agtgtctgca catccagacc caacgacacc aaaccaatca aatggtgtga
     7141 acgatgaaaa gaacaaaaca gcaccagtat ctgcacatcc agatccagta gtccctaacg
     7201 ttcaacaagg agtagaagag aagaaacatg atgcagaaaa gcatgcacct catcatccag
     7261 gaactccaga atctaaagtt ccagaacaac atgatgcaat cgatccttct aaacctgtgg
     7321 aagagcaaaa acatccttct gaccatgcga aagcacaaga aaaacttagt tctgaccaca
     7381 caaacggaca aggaaaactt ggttctgatc atacaaataa ccaaggaaag catgattctg
     7441 aacaggaaga ttcaatgcgc ggtgagctga attatgaaga taaagttgtg caaaaaatca
     7501 tcggaatcgc aattgaacaa gtagacggtt tattatctgc aaacggtggt ttcttctcta
     7561 atgtggcagg aaaacttgtg aatacagata atgtaacggc tgggatcgaa acagaagtcg
     7621 gtaaaaaaca agtagcagtt gatatggatg tcattgtgga atatggcaaa gatatcgaaa
     7681 aaatctttga agaaatgcaa gaagtaatcg gcaaagaagt aaaaaatatg acacatttag
     7741 aagtgatcga agtcaacgct aacgtagtgg acatcaaaac aaaagaagaa tttgaaaaag
     7801 atcaagaaac ggtgcaagac aaagtaactg acgcagcgaa aaaaaccggt gaatttgctt
     7861 cgaatcaaac agataaagta aaatcagcag ctggaagcgg cgcacaaaaa gtaaaagaaa
     7921 atacagaacc acgggttcag taataaggaa gtaaaacatt aggaggaacg aaacatggga
     7981 tttatttggt cacttatcgt cggaggaatt atcggagcaa ttgcaggagc aatcacgaat
     8041 aaaggttctt cgatggggat tattgcaaat gtcgtagcag gtttagttgg atcagcactt
     8101 ggtcaagcat tattaggttc ttggggacca agtattgcag gaatggcaat cgtgccatca
     8161 ttgattggtg ccatcatttt agtactaatc gtatcattct ttgctcgaaa actgtaaaaa
     8221 gacttttaaa aagaggtcgt gaaaaagctg ttcttcatca agtaataaga acagccaaac
     8281 aaaaatagct tctcatattt ttcatttggt tgggcatatt atcgaagatg caagcttttg
     8341 aacactgttt atacggaata agaggctgtg acaagacttt tgtcacagcc tcttatttat
     8401 gcgggcaaat tcccaattag atttttaaaa gtttttcctt aaatgtgtct ttattgatac
     8461 agagaagctt ctttaataaa acaatgcctc cctaaatttt cttgagatag gaaaggtgaa
     8521 catacaaaat aatacgacca ctttgaaaac ccagatgatc cacaaatgga tcgtatgata
     8581 ctgatgaagt aaaaagcaat ggatcagaaa ctcaaagaga ttgaaaaaga caaggaagaa
     8641 ataatatctc ttttttactc ttgtctgttt tagccaataa tagctcgtaa agtaatctag
     8701 acaaacaaat agaagtaaac aaagtttcgt aagagcagca atcgtatcat gttgctctgc
     8761 tagacgattg acgagcagga aaaatgttgg taatggaata tagatcacga gtaatgaaag
     8821 aaaaaagtaa acaataaatg gcatactatt cctcctaatt gtgtgagttc gccctcattt
     8881 taaaataacg atatttgttt gtgaagcttt ttcgtgtcgt aacaaatgtt atctaatgta
     8941 tttttaaata aataatcatt attatcaaaa taatgttaaa aataaaaaag aattgtccat
     9001 cactgttatt caaatcattt tatcaaaact acatagtaaa tgttaaaaag
//
LOCUS       DAZKMB010000111         8969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000111 DAZKMB010000000
VERSION     DAZKMB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.112"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..701)
                     /locus_tag="ACG8V3_002340"
     CDS             complement(21..701)
                     /locus_tag="ACG8V3_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFQ0300820.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
     gene            892..1047
                     /locus_tag="ACG8V3_002341"
     CDS             892..1047
                     /locus_tag="ACG8V3_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300821.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            2214..3182
                     /locus_tag="ACG8V3_002342"
     CDS             2214..3182
                     /locus_tag="ACG8V3_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0300822.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3629..3910
                     /locus_tag="ACG8V3_002343"
     CDS             3629..3910
                     /locus_tag="ACG8V3_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300823.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3912..4328
                     /locus_tag="ACG8V3_002344"
     CDS             3912..4328
                     /locus_tag="ACG8V3_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300824.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTITTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            4939..5373
                     /gene="bacA"
                     /locus_tag="ACG8V3_002345"
     CDS             4939..5373
                     /gene="bacA"
                     /locus_tag="ACG8V3_002345"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFQ0300825.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            6663..7586
                     /locus_tag="ACG8V3_002346"
     CDS             6663..7586
                     /locus_tag="ACG8V3_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0300826.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            7602..8141
                     /locus_tag="ACG8V3_002347"
     CDS             7602..8141
                     /locus_tag="ACG8V3_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0300827.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         3214 a         1453 c         1533 g         2769 t
ORIGIN      
        1 tcccttacgg tatctatgat ttaagctggg attcccaata ataccttgat ttcagtacag
       61 accgaaaacc cgaagagagt gccttctttt cgggttttct tatataatcc tcgaatggct
      121 tccatgcctt taatcgtggt agaggcagtg cgtaaacttc gatagaattt attgcgtctc
      181 tttactggac gatggtcttg ttcaatcaaa ttattcaggt atttaatggt acgatgttct
      241 gtcccttgat aaaagccgta ttcttttagt ttcttaaagg cacttgtaat agagggggct
      301 ttatctgtga ctacaacctt cggttcatca aactgcttca ctaaccgctt aagaaaagca
      361 taggctgctt gtgtgtcccg ttttttacgt aaccaaatat ccaaggttaa accatctgca
      421 tcgatggctc gatacaaata atgccatttt cctttaattt tgatgtacgt ttcatccatt
      481 ttccatgaat aaaaggattt tttatttttc tttttccaaa tttgatagag tagtttgcca
      541 tattcttgca cccaacgata aatcgtcgta tgagaaacgt taatgccacg atcatataag
      601 atttcttgaa cttcacgata gctaaggtta taacgaagat agtagcccac ggctacaata
      661 atcacatcct gctgaaattg ctttccttta aaatgattca tcgtcattcc tcctgctatc
      721 tttttctatt attctacctt atttgatagt agatttaaaa ctttgcaaca gaaccgtttt
      781 gtttatatac aaacaaaaaa tatatggtat tccatagttg ctcatggatt ttataataca
      841 atcggcatat taatctattt gatatctata taagataagg tggcatactt catgaaatta
      901 acaaaactta ctataagttt ggtaattatt gctggtatta ttttatattt tgtattacct
      961 gctaatcaat tttttcttta ctttggaata cctacgattt taatagtaaa tggcgtaaga
     1021 atatttgaac aacgaaaaaa aatgtagctc tacctgatcg ttgcaaagca acaatttcac
     1081 cgaaggtgaa acaagcgaaa ctttacaaaa agtggctagc atttgctagc cttttttgta
     1141 ttgtggagcg tgggttttga ggggggattc aagggggagc ttgcgcccct ttgacaagcg
     1201 gttatttgca ccaaatacgt aggtatttgg ctagcaaata tagagcttgc caaacccttt
     1261 agtgagtttt gtagaaagta cctttaaagg tactttcaca ttacgaacga tacgtccgta
     1321 ggacgtactg aaagggtggg atttatgagc gaactaatgc aaaatttggc tagcaaaaaa
     1381 agttaaaaaa tatatgctaa taatcgagcg aaatgaacac tttttcaagt gagcgaatcc
     1441 gccatgacaa agctgaagca gtttatttca gctttgtcat ggcgtgtgcc tagactttta
     1501 aaaaagtcgg cacacgagca aatatcttta tcaagctcta atttgcgttt taagacgttt
     1561 gaaacgtcta agtatataaa tatataagaa taggtttaag agtgcttaaa tcgaatttta
     1621 gagggcattc agacaacttt agggttgtta gggcttgccc tgaccgcctg taaggcgctt
     1681 taagaacaag ataagggatt tatactaaga ctgtcttaaa acgtctcaaa cgaaggctca
     1741 tagcttctca aagagttcct tatgaatatt tttagcaaag gagccggaag cgactgcgca
     1801 gtataatgtg agagcagagc gatcacacct ttttaagcga ctgtagggag taagattccc
     1861 agttgagaaa aatgcgcgaa tctgtttgga gaaaaaagaa gtggtttttt aggcgggaaa
     1921 ttttgtcccc ctcttttata atctcttttt aaacctcttt ttaaacctct ttaccgtcgg
     1981 ctatatcctt actctcccaa gggaatgacg cttttatcta catcgttttg tctgtttatc
     2041 tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tatctacatc gttttgtctg
     2101 tttgtacatt gaattagtac atcgacgatg ttaatataag atgtagataa ataggcattt
     2161 tgtatttcat aacgtgtatc tcttgtgcga gtaagaaagg agctatttta aatatgtcta
     2221 atgaaatcgt aaagtatcat catgaattaa atacaattcc gttgcgaaaa tttacaccag
     2281 tcgaaatgaa tttgttcttt tctatcgtat caagaatgcg tgatgtaggt gataaaaaag
     2341 tacaatttac attcgagcaa ttaaaagatt taagcaatta taaagcaact gcaaatgttc
     2401 gattcataga tgatttagaa acaacgtatg acaaattaat ggatttacgt tttggtagaa
     2461 gaagtgctga cggtttgcaa cgtgaacgtt ttgttttatt taatcaattt aaaatcgacg
     2521 gaaaatctga tattcctttt gctgaaatac aagttcatga aaaagcctta cccttactta
     2581 ataatcttga agaatgggtg cgctattctc tccaacaatt caatgaatta gaaagcagtt
     2641 attcaaaaac aatgtttcga ttactaaaac aatacagaac caaaggtttc gcttattttt
     2701 caaaagaaga ctttcatgaa ttacttgata ttcctaaaag ttacaaccaa ggtacgattg
     2761 atcaacgtgt gttcaatcca atacgacaag aactaactgc aatctttaaa ggtttaacaa
     2821 tcaaaaagaa atacggaaaa ggacggggaa agcctgtcat tggctatcaa tttacattta
     2881 aacctgagat aaaaaaatca gacgacttta acaaacatgc cccaacaaaa gaagaacaaa
     2941 acaaagcatt agaactagct aaccaacaag ttcaatctga aaaagcacct acttcattac
     3001 aagaaaaaat caaagaacaa aagcgaaaat tggaactttt acaacagtta gaaaagttag
     3061 aaagagaaca aaatacccaa aataaagaaa ataatgcaca aaaatcagag ataaattccg
     3121 atattccttc agaactaaaa aaagacttat tagacaatct tcaaaactta ttcaaagatt
     3181 gaggggtttt tagattttat gacagaaaaa acaatcaaag acggagaaaa aatggtggca
     3241 attctggcaa taaaaccagc aatttttatt aatttaaatt gctggtttta ttcttctttt
     3301 tgcttttgcg agcagagcaa catggaccca tgttgcgata ttttggtggc gccccaatcc
     3361 ccgttttttc gttgaatctt actcggactg cactcgtttt acagctgatt tttctgaatt
     3421 ttgaaagagc ataaggaaac gtggtatcac tttcttacaa aaggtgctag ataggctgtg
     3481 cctatcacgt ttctcagaaa aaaatttgct atcacttttt tgccattgtg gctaggctat
     3541 gtatagtgtt ttggcgaaaa atagctatac aaatactata tttgtatgat atacataaat
     3601 tatacgctga tacgaaagga aaatttccat ggacatttta gttcgaaata tcaatcccaa
     3661 aattattgca agaatagata aacgttgtca ggaactcaca accaaaacgg gcaaaaaatg
     3721 gagtcgaaat aaatatctaa aagttctaat cgaaagtgat tttgaccgtc aacttatgaa
     3781 ttacaaaaaa gatcaattcg atttattact cgaaaaattt attgccattc aaacagaaaa
     3841 tacgaacata ctgaatgact atgttcacac aaacaaccaa ctcattgaaa cacttatcgg
     3901 aggaatataa catgagaaaa acttctgttc aaaaacacgt aaccctttat cccagtcacg
     3961 ttgacgtttt agattccatc atcgaaaaaa atccagacgt caacaattat tcagaagcca
     4021 ttcgtttttt atgtatgagt tatgaacctg ataccaatac aatcacaacc agtaaccaag
     4081 caaaactcaa tgccatggga aaagaaattt ctatcatgac agaaatcatg gcagaatttg
     4141 ctgatttata tctaaaagat actggtgttc ttatcggtac aaattctaaa gtgtatcaag
     4201 aagtgaaaga aaaaattgaa aatcaactca atccttcccg acgaaatcaa cccaaaaatc
     4261 gtcaaaaaga aaccaatcaa acctttactt ctggaaattt tgacccaaca aactttctaa
     4321 aaagataaaa aaagaaaccg atccctaaaa aaattcctcg atttcttttt agggatcagt
     4381 cttccagcaa gatttgcaag ttggtaccaa cttgcgaaaa aaaagaaaaa tccgcttatg
     4441 caacttcttc tttttgtatt ttggggcgct accccaatcc ccaagtatcg gttatttaat
     4501 agcgcggaaa tccaaagaat aggacgtacc ctgaaacaaa aaaagactgc tctcacgagc
     4561 agctaaaaat gagattatcc tattcattaa cattagaaaa gaagagattg aggtataaaa
     4621 agagcatatc aaatgctcac aaaaataaca aatctctaaa aaatgaccgg tcaaaatgca
     4681 caaagaaatg aaaaaagaaa agtgcataaa atcaagcatt ggctgaagtg tttaagctag
     4741 accaattaga aatcacaaag tgagcgtctc tatccattgg tagaggggct acttggcata
     4801 gtgtacatta tgtctactaa cctttaaaat aacacgtttt ataaaataaa ccctcttgac
     4861 aatatttaat tttctgatta ttctaaatat atacagaaag cgctttccta ggattttata
     4921 tctaaggagg aagtaacaat gaaaaataaa attattaaaa ttattacttg tctatttttg
     4981 ttcggtatga ttgttggagc aagtactccg ttaaatctgt ttccttttag tgctagaggt
     5041 ataaaagtgg aagctgcaag ttcacgatat aatcataatc atcgtggatt tacttgctat
     5101 aaaatgaact attatgttac taataaaatg tgtagagatt tgaaaaaaaa ttataataag
     5161 ctaaagactc ctgctcaaat tgcatctttt attcctattg gtggagttgg aacatgggtt
     5221 attacaaatg cttttagtag tgctgttgat gcaatgaatg tctttgtaag agctgcaaat
     5281 caaggaaaag gtgtacaatt aacatataat gctcacttta gcaatacaac atcatatcaa
     5341 tacaatgatt acgctcgtta tgtaataaaa taatcagaaa gtgaaactat ctcatgaata
     5401 aaaagatttt agctgtaatt atttcaataa tagttttaat cactatgttc ttcatattta
     5461 ggctaatttt caatataagc ttacaaaaaa gtattcttta tctaattcct atagcattta
     5521 tcttagcaat ttttagaagt atttacggaa ataaaaactc taaatagcta aaaataaatc
     5581 atactaaatt ttacaaaaag tggctagcat tttctagcca ctttttgtat tgtggagcgt
     5641 gggttttgag ggggattcaa gggggagctt gcgccccttt gacaagcggt tatttgcacc
     5701 aaatacggag gtatttggct agcaaatata gagcttgcca aaccctttta gtgagttttg
     5761 tagaaagtac ctttaaaggt actttcacat tacgaacgat acgtccgtag gacgtactga
     5821 aagggtggga cttatgagca aattaatgca aaatttggct agcaaaaaag ctaaaaaata
     5881 tatgctaata atcgagcgaa ataaacactt tttcaagtga gcgaatccgc catgacaaag
     5941 ctgaagcagt ttatttcagc tttgtcatgg cgtgtgccga cttttttaaa agtctaggca
     6001 cacgagcaaa tatctttatc aagctctaat ttgcgtttta agacgtttga aacgcctaag
     6061 tatataaata tataagaata ggtttaagag tgcttaaatc gaattttaga gggcatttag
     6121 acaattttag ggttgttagg gcttgccctg accgcctgta aggcgtttta agaacaagat
     6181 aagggattta tactaggact gtcttaaaat gtcttaaacg aacgctcaga gcttctcaaa
     6241 gggttcctta tgaacggctt ttagcaaagg agccggaggc gactgcgcag tataatgtga
     6301 gagcagagcg atcacacctt tttagcgaac cgtagggagc aaggtttgga attggcagtt
     6361 ttgataaatt cgcgaacctg tttagagaaa aaaggagtga ttttttaggc gggaaaatta
     6421 gcctcccctc tttttatatc tctttttata tctcttttta tatctctttt atgtcggctg
     6481 tgtccttact ctcccaagca attcagagtt ttttcaagta ggttttctac gcttttcaag
     6541 taggttttct acgcttttca agtaggtttt ctacgctttt caagtaggtt ttccgacatt
     6601 attgagtatt tttataaagt atgttacttt tatttcatac tttataaaag taggagaagc
     6661 ttatggcaaa tgaaattgta aaatatgaaa atagattaaa tcatattcct ctgagaaaat
     6721 ttaatagtcg tgaaatgaac ttattttttt caattgcttc acgtgtacgt gaaaaaggtg
     6781 tagatgagat tattttttca tttcacgatt tgaaaaaact aagcggttat acagaacgtg
     6841 gggaacaatt tataaaagat cttagtagca cgtatgacaa attattaagt attaatgctt
     6901 ggaatgatga tggaaatact ttaactaaat ttgttgcttt cacaaaatat agcattatac
     6961 gagataaaca acttgttata atttcagtta atccagaatt taaaggactt tttaatcaat
     7021 taaagaactg gacaagattt aatctagagc aatttactaa tttggatagc acatattcaa
     7081 aaactatgtt tcgattatta aaacaatatc gaactcaagg ctggactgaa ttttccaaag
     7141 aaaaattttt tgatttacta gatattccta aaagttattg gaatagtccg tcaaatattg
     7201 acaaatttat cttaaggcca attaaagaag aactcactcc cttatttaaa ggattaacta
     7261 ttcgtaaaaa atatggtaaa ggtcgaggta aacctgttat cggttatcgt tttacttgga
     7321 aagctgaaat taaccatgct gacgattttt ccaaagggaa acaagaagat ttacgtataa
     7381 aattatttaa tattgatcac aatggagaat taacacgaga agaaaaatgg cgcgctaaag
     7441 atcgaatact aaatcttccc ttaggcactc atgaagctga ttttaaacaa caacaacaaa
     7501 ctgaagaaaa acaggcagaa aaacgagaac tttcaaacaa actcaaacaa gatttactag
     7561 gaaatttaca aaacttattc gattaagctt aaggagtatt tatggcagat aaaacaatca
     7621 aagaacttgc ggaagaatta ggagttagta aaacagcaat taacaaaaaa gtttctgaca
     7681 aaaatcgaaa actttggttt gcaaaaaacg gaaacaagtt tgtgattaac gaaaccggtc
     7741 aaaaagctat aaaatcaatg tttttagtcg ataacgcaaa cttaaatcga aaactagttg
     7801 gcgaaaaatc aaaaaccaaa aaccataaaa acaccaacca agtttccgaa ttaaaactcc
     7861 tttctttcct agaaaaacag ttaaatcgaa aagatataca gctacaagaa aaagataatc
     7921 aaataaaaca aatgcaaaaa ttactcgacc agcaacaaca actcactcta caagcgaacc
     7981 aacaaattga gaacctacaa gaacagctcc atttaactta tacagaagaa actccaacaa
     8041 ccgaatcaac tgccttttct gaaaaaaaag cagacaatac acaaccactt attgaaaaaa
     8101 agtggtggca aatttggaaa tctaatagaa ccaatctcta aaattttcta gaagttagtt
     8161 cggatagtag aggaaaaagt tgtactacct gatggggaga tcactcaaat ttttatgaga
     8221 aagaaaaaat agtttacaac tgtaaacaat caatgtttat agttgtaaac tatcaaacta
     8281 tatggtgact tattgcttaa aataggttga aatctcactc aatttttggt ggaaaaacaa
     8341 aataaattct aatcgatttt gttgaaaaag tcataaaaaa acaaatgtga ttttaacaaa
     8401 atttcaaaaa aatcgtttga aatttcgttt attttttttg agaattttga tcatcgtagg
     8461 gtcattgtag aatcattatg agatcatcgt gggatcatcg aataaagtat gggaaaggct
     8521 tgcggtgctt ggtattaagg gcattctccg cctcccaggt tcttgaccat tttctcttat
     8581 aagtgggatt tttaccacgt tgagagaaaa tggtcaagaa cctgggaggc ggagaaaaca
     8641 taccaagcac cacaagcaaa aaaagttaga gtaacttcca aaaaagaaaa attttttaga
     8701 acaacttttg aaaatttaat tttgatttat atccgttcaa tttaaacatt tgataaactg
     8761 aaggctagta tttaaaatgt gtatacaagt ggtagcgtta aaaaaagtgg aggtataacc
     8821 tatgttaaaa aaaactgtgt tggtactggg ttctatttct ctaggaatat taggttttag
     8881 ccccatgcta ttatccgtaa ttggaaaaaa caattccaac agagaactta aattgaattc
     8941 aaattattac aacagtccta acgaagaat
//
LOCUS       DAZKMB010000112         8851 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000112 DAZKMB010000000
VERSION     DAZKMB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.113"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..2724
                     /gene="adhE"
                     /locus_tag="ACG8V3_002348"
                     /gene_synonym="adhC"
     CDS             127..2724
                     /gene="adhE"
                     /locus_tag="ACG8V3_002348"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0300828.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2953..4059
                     /locus_tag="ACG8V3_002349"
     CDS             2953..4059
                     /locus_tag="ACG8V3_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0300829.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4076..5104
                     /locus_tag="ACG8V3_002350"
     CDS             4076..5104
                     /locus_tag="ACG8V3_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0300830.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5110..5589
                     /locus_tag="ACG8V3_002351"
     CDS             5110..5589
                     /locus_tag="ACG8V3_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0300831.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5605..6900
                     /locus_tag="ACG8V3_002352"
     CDS             5605..6900
                     /locus_tag="ACG8V3_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0300832.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6911..7735
                     /locus_tag="ACG8V3_002353"
     CDS             6911..7735
                     /locus_tag="ACG8V3_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0300833.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7900..8799
                     /locus_tag="ACG8V3_002354"
     CDS             7900..8799
                     /locus_tag="ACG8V3_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300834.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
BASE COUNT         2838 a         1536 c         1965 g         2512 t
ORIGIN      
        1 gcaattttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt acatagaaaa
       61 ataaatattg tatgatatac atgtgaatta tttaacaaat aaccatgacc cattaggagg
      121 ataatgatgg ctaaagtaat agaaaaagaa aaaacgaaga caattgacgt acaagcaatg
      181 attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag ctttgatcaa
      241 gaaaaagtag accatatcgt gcatgaaatg gcaatggcag ctttggatca acatatgcca
      301 ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa agcaatcaaa
      361 aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac agttggtgtg
      421 atcaatgaag atgtccaaaa agggttgatt gaaattgctg aaccagtcgg tgtggtttgc
      481 ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat gatcgcgttg
      541 aaaacaagaa atccaatcgt ttttgctttc catccaagtg cccaaaaatc ttctgcagaa
      601 gcagcacgag tggtacgtga tgctgctatc gctgccggag ctcctgaaaa ttgtatccaa
      661 tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc aggtatcgcg
      721 atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac aggaaaacca
      781 gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc aaaaattaaa
      841 cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat ttgtgcttct
      901 gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga attccaagca
      961 caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc agtgatgaac
     1021 gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga aatcgcaaaa
     1081 ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt ggaaggcgta
     1141 ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat gatcaaagct
     1201 aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg aggattagga
     1261 catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg tcttcgaatg
     1321 aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg tgatatctat
     1381 aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa ctctgtttct
     1441 cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg gagaaataac
     1501 atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct attgtactta
     1561 gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat ggtccaattc
     1621 ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt gaagatcgaa
     1681 gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg aacgaaactg
     1741 atggcagact tcaagccaga tacagtaatc gctcttggag gcggttctgc aatggacgca
     1801 gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg tgcaaaacaa
     1861 aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa aacacaattc
     1921 gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc ggtcatcact
     1981 gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc agatgtcgca
     2041 atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga taccgggatg
     2101 gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga ttatacacgt
     2161 ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc agtaaaaaca
     2221 cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg tatggcgttt
     2281 gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg ggaatacggg
     2341 atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta taatgcaaaa
     2401 gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga tacagattac
     2461 gcagatatcg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt agtcgaagct
     2521 ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag tctaaaagct
     2581 caaggagtca cacaagaaac actagataca accgtagacc gtatggctga attagcttac
     2641 gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt gaaacagatc
     2701 atcattgatg cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata agaaaataga
     2761 tgaaaaagaa cttggaaaac gttgtttctc caagttcttt ttttaatttc caagtaactg
     2821 aaagttactt ttgttattta acatttaggt caattttaga cagtttttgt tcaaaaagcg
     2881 atatttatag tggacggtat tactgctata ttaaggatat aaaatgaaag cgtttaaaaa
     2941 ggtggcgtaa agatgattat taaagaagag atttttgtac ctaaagaatt agtgaaagaa
     3001 aaaattgacc tgctcgttga aaacctgaca acgatcaaag acgataatgg tgaatttttg
     3061 ttggactttg atggactgaa agtagatgac aaaagctgga cagtgtggaa ttggccacaa
     3121 ggtgtcggtc tatatggtat ttataaaaat taccagatga caaaaaatcc tcgcgcttat
     3181 caagtggtca atgaatggtt tgaggatcgt atggaagaag gcgctccgcc caaaaacgtc
     3241 aatacaatgg caccgctatt gactatggct tacttgtatg aagacacgaa agattcacgc
     3301 tatttgcctt atttagaaca atgggcagaa tgggtcatga atgacatgcc tcggacaaat
     3361 gaagacgggt tacagcatgc cacatatgga ccggaaaata agaatcagtt gtgggacgat
     3421 acattgatga tgacagtgct tcctttggca aaaatcggga aactgttgaa tcgcccagaa
     3481 tatttagaag aagctcgtta ccagtttatg atccatatca aatacttgat ggataaacga
     3541 acaggattgt ggtatcacgg ctggacgttt gaaggaaatc ataactatgc cgaagcattt
     3601 tgggctcgtg gaaactgctg gttgacgatt gctattccag agatcattga aatcttagaa
     3661 ttatcgaaag aagatgcatt gcgcaaatta ttgatcgaaa cacttgaagc acaagtgcgt
     3721 gcattaaaaa cttatcagtc tgaaagcggt ttatggcata cgttgttgga tgatccttct
     3781 tcttacgtag aatcttctgc aactgcagga tttgcttacg gtattttgaa agcggtccat
     3841 aaacgttatt taccacaaga atataaagaa gtcgcttatc gtgctatcca aggattgctt
     3901 gaacaaatcg atgaaaaagg agaagttcaa aacgtttcaa tcgggacagg aatgggggac
     3961 agtttagatt tttatcggaa tatcggaatc acagcaatgc cttatggaca gtcgttaacg
     4021 gtgctttgtt tgacagaatt gctcgtctct tattgttaaa agaaaggaaa cccatatgaa
     4081 aaaaacatca ctgctgtttg cagcaagttc gcttagccta ggtctgctag gtctaagcgg
     4141 ctgcggaaat aattctagtg tggcaactgt cgcagatgca gatgtaaatg agatagttac
     4201 cttgcgcttt gcttatgcaa gcaacagcca gccagtaatt gatgcaatga atgaatttgg
     4261 gcgtttagta aaagaaaaaa cagatggttc cgtacagatc cagtatttcc cagatggaca
     4321 actgggtgga gaacgtgaac tgatcgaatt gacacagtca ggagcagtag atttcacaaa
     4381 agtaagtgcc tcagcgttgg aaagtttctc taaagattat tcgatttttt cagttcctta
     4441 tctattcaat gatgaagaac atttctttaa ggtgatggat aatgaagaaa taatggagcc
     4501 aatctaccaa tcgaccaaaa atttaggatt taccggattg acctattatg attctggtca
     4561 gcgcagcttt tatatggtag acggtccgat ccatacgccg gaagatttga agggaaagaa
     4621 gatccgtgtg atgcaaagcg aaaccgctat caaaatggta gagcttttag gcggttcgcc
     4681 cgtacctatg ggaagtgatg aagtgtacac gtctcttcaa tccaatttga tcaacggatc
     4741 agaaaacaat gaatttgtct tgtatacagc tggacatggt ggtgtggcta aatactattc
     4801 ctatgatgag catacacgtg tgccagatat catcatcatg aatgatgcca tcaaagaaca
     4861 gctaactgat gagcaggaaa aagccattga agaagcagcg aaagaatcaa cggtttttga
     4921 aattgaagct tttgcaaaag caattgaaga agaaaaagct ttagcacaag aagaacatgg
     4981 agtccaattc aatgatgtag ataatacacc gtttcgagag gcagttgccc cacttcatga
     5041 tatattcaaa aacgatccta cctacaaaga cctttatcaa aagatccaag agcaaggagc
     5101 gtagctgtga tgaaaatgat tttaaacaag gtattagaga tcctgggagc tgttttgctt
     5161 gttgggatgg tcgctgttgt tttatggcaa gtattttcca gaacggtttt aggaaatcca
     5221 aatacggtca cggaagagct tgttcgattc ggtcttgtct ggtttgccat gttgtcttcc
     5281 gcgtatgttg tgggacaaaa agcacattta gccgtaacgg ttttaagtga aaaattaact
     5341 ggcaaaaaaa gtaaaatttt agaagttgtt gttcaaagtt tatttctggt atttgctgcg
     5401 atgatcatgg tgtatggcgg atggaatgct gtcacattga caatggggca gatctcgcct
     5461 tctttaagta ttcctatggg atatgtttat ctgtcagtac ctgtatcggg ggtactcatc
     5521 attatctata gcctgatcaa tctaatcgat acattaagca acaagccagc aattcaagaa
     5581 aaaatataaa aatggaggaa taaaatggca atagaagccg gattagtgtt attttttgta
     5641 tttgttgtgt tgcttgtatt agggatgccg attgctataa gtattgcatt ctcatcgatg
     5701 atgactttat tgttagttat accgtttgat gtttctgctt ttagttcagc tcaaaaaatg
     5761 gtgggaagta tcaatagttt ttcgctgatc gctattccgt ttttcgttct ctcgggcatt
     5821 atcatgaata atggagggat agctaagaaa ctagtggatt ttgcgatgtt gttcgtcggg
     5881 cgcgtgccgg gggctttggc tcatacgaat gtgttaggga atgctttatt tggttccatc
     5941 tctagctcgg ccattgccgc ttctaccgcg attggcggag tgttgatccc tcagcaagtg
     6001 gaagaaggat atgaccggaa gtttgcgaca gcggtcaata tcgcgtcagc tccgacaggt
     6061 atggtcattc ctccaagtac tgcttttatc atgtattcac ttgttgctgg tggtgcttcg
     6121 atctctgctt tgtttatggg aggttatctc gtaggcgctt tgtgggcgct aggaatcatg
     6181 ctagtagcat tcatcgttgc taaaaagaat aaataccctg ttgtcgaaaa aggacagatg
     6241 gctcatgctg gaaaaataat aaaagaggcg attcctagcc ttctattgat tgtcatcatt
     6301 atcggtggta tcttgacggg gatatttaca gcagtagaag cttcagcgat agctgtcatt
     6361 tactccctgt tgattgctat gttttactat aaaacggtaa aactaaaaga tatacctaat
     6421 atgttgtttc aatcagtagc aatgtcaggg acgatcatgt tcttattggc tacttcttcc
     6481 atgatgtctt tcgccatggc gtttaccggt attcctcagg caattagttc gttgattatg
     6541 ggcgtaacgg ataataaatt tattattctg ttattagtca atcttgtgtt attactggtg
     6601 ggtatgttta tggatgtggc gcctgctatc ttgattttta caccaatatt tttaccaatc
     6661 gtcacaagtg taggggttga tcctgtacat tacgggttgt tttcaatcat gaatctgtgt
     6721 gtaggctcca tcacgccgcc agtaggtaca ggactatatg tcggtgcaag tgtaggaggc
     6781 gtaaaagcag aggcaatgct gaaaccgctt cttccttttt atggggttat cttagtcgtc
     6841 ttgtttttga ttacttactt cccgcaaatc gtcatgtggc tacccaatat gctgagttag
     6901 gagaatagtt atgttgaaca ttacaattag aggacatgat ctttccgaag tccaaacgat
     6961 agaagatctt gcagaaaaaa caaaagaaca agggattcat acactgcagc tcgccttagg
     7021 tctttcgttt cctgaaatac cttctggcgc aaatgagatc aattctggga tgggaaatta
     7081 tgtcaaacga gttttagaaa agcaagaagt gaccgtagga attttaagtt gctatatcaa
     7141 tatgattcat ccagatttaa cgatacgaca agagttgctg acaaagttcg agcagtatct
     7201 tcgctatgct tcttcctttg gtgcgtcgat ggttgcttcg gaaactggat gtgttttgcc
     7261 ggaaatccaa tatacggaag aaaatttcac agatgaagca tttgctgaag ctgtgtccgt
     7321 tatccgtcgc ttggtaaaag ctggagaaaa ataccaaatg atggtgggga tagaaccagg
     7381 tttgaatcat ccggtgtatt ctttagcaag ggtagagcag ctgatccaag cagtagattc
     7441 ggactatcta ggaatcattt tagatccgac caatttgatc acaagtagtg attatcaaga
     7501 acaagtccaa ttggtagaag aagcttttga acgttttggt gaaaagatat gtgcggttca
     7561 tttgaaagat tttcgtgtcg aacaggaaaa aatcgtgccg gtaaatttgg gagacggtgt
     7621 gatcgagtac acaaaaatca aagaaatcat catgaagaat cgtccgtacc tgtatgtggt
     7681 tcttgaagaa acaaaagatg atgggattcg ttatggaaga agtcttcttg aataatgtaa
     7741 agaagacgtt ggttaactaa tatttagaat ggagggggcg gggctagaag atgccacgtc
     7801 tcttcttttt tataagagta tataaaagca gctctttttg attacaaaaa acgtggtata
     7861 ataaaattgt gcataagtga acaaaaggag gtgcggcaca tgctccaaaa gaaaatcagc
     7921 ggtgaacaag tcaatacgat ggtttctgat cagtatgaaa tctatcatgt caaagatgtc
     7981 gtcagccaaa ataaaacgat ctatcatcat catgatttct atgagatcca tgctacatta
     8041 aagggagaag cgacttttta tttgaacgga cgacaattta cgatcaaagc aggaagtgtg
     8101 ctgctcatcc attccaatga tttgcatcgt atcgtccgcc aatcttcaga tgtattcgag
     8161 cgtgtctata tctttgtcac cccttctttt ctagaaagtc gttccacgcc atggtctaat
     8221 ctatcggctt gttttcaacc aatgggagag aagaggagtc gtgtattgca aatagaacag
     8281 gaagtattga agcaaaaatt atcatttatc gaccatcctc ctgataaaaa aatttacggt
     8341 gaagacataa aatatgaaca agctctggta gaatacttga tttttctcaa tcaagttgta
     8401 caaaaagaag agagtgcgca agaacagaat gctgtagttc ctaatgagcg tgtggaacaa
     8461 atgttacaat atatctcaaa aaatttatct catccgcttt ctttagagga aatggaaaag
     8521 cagttttttg tcagcaaata ccatatcaca cgagaattta aaaagtacac tggatttacg
     8581 tttcatcagt atgtaatgaa gaaaaagctg atctatgcca agcacttgtt gcgagaatac
     8641 cgcagctcta gtgctgtata tagtaaaagt ggtttttctt cttatccgca ttttctgaaa
     8701 gcattcaaga gagagttcgg cataacgcct aaagaatttt tgaaacgtga tacacagaaa
     8761 cagttggttc attatgaaca ttttgaagat gaacaatgag aatgatcact aaaaaataac
     8821 cgcagtagct gctgttgttt acatagaagc a
//
LOCUS       DAZKMB010000113         8494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000113 DAZKMB010000000
VERSION     DAZKMB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.114"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8V3_002355"
     CDS             complement(<1..149)
                     /locus_tag="ACG8V3_002355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0300835.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8V3_002356"
     CDS             complement(593..1714)
                     /locus_tag="ACG8V3_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0300836.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8V3_002357"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8V3_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0300837.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8V3_002358"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8V3_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFQ0300838.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG8V3_002359"
     CDS             complement(4304..5215)
                     /locus_tag="ACG8V3_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0300839.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG8V3_002360"
     CDS             complement(5232..6239)
                     /locus_tag="ACG8V3_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0300840.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG8V3_002361"
     CDS             complement(6236..8365)
                     /locus_tag="ACG8V3_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0300841.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG8V3_002362"
     CDS             complement(8370..8480)
                     /locus_tag="ACG8V3_002362"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300842.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZKMB010000114         8334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000114 DAZKMB010000000
VERSION     DAZKMB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.115"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..1040
                     /locus_tag="ACG8V3_002363"
     CDS             168..1040
                     /locus_tag="ACG8V3_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0300843.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1037..2995
                     /gene="nagE"
                     /locus_tag="ACG8V3_002364"
     CDS             1037..2995
                     /gene="nagE"
                     /locus_tag="ACG8V3_002364"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0300844.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3327..4178)
                     /locus_tag="ACG8V3_002365"
     CDS             complement(3327..4178)
                     /locus_tag="ACG8V3_002365"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0300845.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4301..4768
                     /locus_tag="ACG8V3_002366"
     CDS             4301..4768
                     /locus_tag="ACG8V3_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0300846.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5031..5513)
                     /locus_tag="ACG8V3_002367"
     CDS             complement(5031..5513)
                     /locus_tag="ACG8V3_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0300847.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5613..6476)
                     /locus_tag="ACG8V3_002368"
     CDS             complement(5613..6476)
                     /locus_tag="ACG8V3_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0300848.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            7074..7949
                     /locus_tag="ACG8V3_002369"
     CDS             7074..7949
                     /locus_tag="ACG8V3_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0300849.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(8094..8246)
                     /locus_tag="ACG8V3_002370"
                     /pseudo
     CDS             complement(8094..8246)
                     /locus_tag="ACG8V3_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT         2578 a         1686 c         1485 g         2585 t
ORIGIN      
        1 attgattacg ttaacaataa ttcgtttcat gtgactagat catactaggc atgaaaaatc
       61 tctgaggaac atggtttccc taggaggttt ttcgtgtcta tttttgtttg gccgcaatca
      121 actggtctag caaacgtgaa aaccaaatct tcaaaaaggg tgcgcgcatg aaaatcaaaa
      181 agatctttaa tcaaaatgca gtattggtag atgacggtgg acaggaaaaa gtagctattg
      241 gaaaagggat tggatttgac agaaaaagaa atgatttgat ttttgatcgt gatgtcgaac
      301 ggatatttgt catggaacca gaaggtcaaa tcaagttgca aaatttactt aatcagattg
      361 atgaacgctt tttatttgca gcagaaacga tcattgatca tgcggaaact gttttgatgg
      421 aaaagttgaa tgaacatgta ttgatcgctt tgaccgacca cattgctttt tctgcggaga
      481 acattaataa cggtatcgtc atccgcaaca aacttttaag agaaatcgaa gtgctttaca
      541 gtgatgaatt cagtattgct caatgggcag tagagtatct gacaaaagag cttgggattc
      601 catatactta tgacgaagca ggatatatcg caatccatat tcacagcgga cgaagcggtc
      661 ggaacaataa ccatcgcagt atccgtgaag tgacgattgt atctgacatc atccatttag
      721 tggaatcaga attggaaaca gatatccatt cagaaccatt ttcactgaat tattcaagac
      781 tggtgaatca tttacgactg cttctacaac gaacccatgc gcaacaatat gctgttttag
      841 acacggaaat cgtagaaatg gtcaaacgaa aatacccaga aagctataag atttctaaaa
      901 aaatccgtgt gttgctgacc aaagaatacc agctcgcgat cacgaaagaa gaactgggtt
      961 acttggccat ccatatcgag cgtttaagaa gcgcaatcgt acaaacaaat gttaacaatc
     1021 atgaggagga aaaaaattga aagcatatct acaacgaatg ggacgttccc ttcaacttcc
     1081 agttgcggtc ttacctgctg ctgctttact agtagggatc ggaaactggt gggcatctta
     1141 tagtaacgac attgtcgctc attttttaca agctggcggt aacgcagttt taggtcaatt
     1201 acctttgctg tttgccgttg ggttagcact agggatgtca gtggacaaat caggtgctgc
     1261 tgccttggca ggtttggtcg catttgaagt tccagtaaat gttttaaaaa ctgattcagt
     1321 agctacttta ttgaatatca aaccagaatc agttgatcca tcttttgctc agatcagtaa
     1381 cgtatttatt ggtattattg ccggattgat tgcggcagct ctttataatc gtttccacga
     1441 agcaaaatta ccgatggctt tatctttctt tagcggcaag cgtctagtgc caatcttggc
     1501 tgcctttgtc atgttgctga tcagtgcagt gctactgctc gtatggcctc cggtttactc
     1561 tgctttagtt tcttttgcta aattcatttt gagtttaggc tggggtggtg caggtcttta
     1621 tggcttcttc aaccgtttac tgatccctac aggattgcat caagcaatga actccgtgtt
     1681 ctggtttgac gtagcaggaa tcaatgatat tggtaaattt ttggcttctg aaggaacaaa
     1741 aggcgtaaca ggaatgtatc aagctggatt tttcccaatc atgatgttcg ggcttccagc
     1801 aggagcatat gctatttaca gaaatgcacg tccagaaaac aaagcaaaaa cagcttcctt
     1861 gatgttagca gcagcttttg cttcattctt tactggtgta acagaaccac ttgaattctc
     1921 atttatgttc gttgcttggc cattatatgt cttgcatgca attttcactg ggatttcttt
     1981 agcagtgagt gcattcttcc attggacagc aggatttgcc tttagtgccg gatttgtcga
     2041 ttacttccta tctttgaaaa atccgattgc gaatcagcca ttgatgttga tcgtccaagg
     2101 attagtaaca gcagtgattt actactttgg ctttaacttt gctatcaaaa aattccatct
     2161 gatgactcct ggccgcgagg aagcagatct ttcagatgac gatactgcaa cgactaattc
     2221 agacaataaa tatgcggcac aagcaagcaa aatctacgct gcattaggcg gagccgacaa
     2281 tgtaacttcg atcgataact gtactacacg ccttcgtttg caagtcaaag atacaggcct
     2341 gatcgatcaa aataaaatca aagccactgg cgtacctggt atgaagatca tcgacggaaa
     2401 aaatgcgcaa gtcatcgtag gtacagaagt acaattcgtt gcagatgaga tggcaaaact
     2461 acatggtggt gctgcaacac gacctgcaca aacaaacacc gtggttaaaa cagaaacatt
     2521 tgccggtgga acgatcgaac aagatattta cgctatcgca aatgggaaac tgattccaat
     2581 cactgaagtt tcagatgatg tattcgcgga aaaaatgatg ggtgacggct atgctgtatt
     2641 accagaaaat ggagaaatct tctctccaat cgctggtacg atcacaaaca ttttcccaac
     2701 gaaacatgct gtcggaatcc aaacagatgc tggtatcgaa gtcttgcttc atatgggcat
     2761 caacactgtt gacttaaaag gcgaaccatt cactttatat gtaaaagaag gtcaaaaagt
     2821 cgcaagaggt caattgattg ctttagttga tcttgccgct attcaatctg caggcaaaaa
     2881 cacggatatg gtcgttgtat tcacaaatgg cgacaaagtc caaagcttag aaatcgaacc
     2941 cgcacgtgat gtaaaagcaa atgataaaat cggctctgtt tccaacaaag cctgagccat
     3001 cgtcttcccc agactttttt atcgataaac aaacaggagc tgagacaaaa atcttgtctc
     3061 agctcctttt tccgaataaa aacggcgttc tcctgccaac tccgcggtaa taagtccagc
     3121 caaacgtaac atatgaagag ctatttttta tttgactctt cttattactt gatgcagaac
     3181 agctttttta caacctctga taaacggtgt tcaaaaacca gtttatcggt atgaagtcaa
     3241 atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttcatact caaaactaat
     3301 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
     3361 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
     3421 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
     3481 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
     3541 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
     3601 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
     3661 tcacgtttca atacccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
     3721 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
     3781 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
     3841 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
     3901 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
     3961 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
     4021 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
     4081 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
     4141 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
     4201 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
     4261 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
     4321 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
     4381 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
     4441 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
     4501 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
     4561 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
     4621 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
     4681 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
     4741 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
     4801 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
     4861 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
     4921 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
     4981 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
     5041 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
     5101 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
     5161 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
     5221 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
     5281 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
     5341 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
     5401 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
     5461 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
     5521 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
     5581 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
     5641 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
     5701 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
     5761 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
     5821 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
     5881 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
     5941 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
     6001 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
     6061 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
     6121 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
     6181 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
     6241 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
     6301 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
     6361 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
     6421 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
     6481 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
     6541 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
     6601 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
     6661 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
     6721 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
     6781 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
     6841 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
     6901 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
     6961 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
     7021 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
     7081 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
     7141 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
     7201 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
     7261 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
     7321 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
     7381 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg caatttatcg
     7441 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
     7501 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
     7561 tcatggagat gcgaaagttc atccaagaag cagttgaatc gctcattcag caaaatgcct
     7621 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
     7681 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
     7741 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
     7801 tcgatcatac tgacgcctct tattcaaaac gctactccat aaaaatcctg aaagaagact
     7861 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaaact aaagaaacgg
     7921 taaagaaaga cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca
     7981 caagaaatac agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa
     8041 gacttaacgc gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag
     8101 tcatgacaaa tttccttcgt tgctgggtag attttccggc attgttgata gattttatga
     8161 tacttttcta cattttgtaa gagaggttcc actgcatctg tatattggat aaaattctga
     8221 caacaacttt caggtgtgtc gaaccactct aatctttaaa gataaatatt aaaataacga
     8281 ttaataatat taacagtctt tatttttcac caccaaaaac ctatctttgc atat
//
LOCUS       DAZKMB010000115         8066 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000115 DAZKMB010000000
VERSION     DAZKMB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.117"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            311..2077
                     /locus_tag="ACG8V3_002371"
     CDS             311..2077
                     /locus_tag="ACG8V3_002371"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0300850.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2179..3438
                     /locus_tag="ACG8V3_002372"
     CDS             2179..3438
                     /locus_tag="ACG8V3_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0300851.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3585..4886
                     /locus_tag="ACG8V3_002373"
     CDS             3585..4886
                     /locus_tag="ACG8V3_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0300852.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4889..5740
                     /locus_tag="ACG8V3_002374"
     CDS             4889..5740
                     /locus_tag="ACG8V3_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0300853.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5753..6568
                     /locus_tag="ACG8V3_002375"
     CDS             5753..6568
                     /locus_tag="ACG8V3_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0300854.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6719..7885
                     /locus_tag="ACG8V3_002376"
     CDS             6719..7885
                     /locus_tag="ACG8V3_002376"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0300855.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2629 a         1384 c         1601 g         2452 t
ORIGIN      
        1 taaaaaaatt aggctagtct aaattattat gtttcattct tctagaatat acttctacgt
       61 taaaaactgt taaaaaactt ttactttaga aaattggaga aattgatcgc ttaagataaa
      121 ttcaggacac taaatagtac aggaagtaga ttaaaacttt taaaagtttt aagtctatct
      181 atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga taacattttt
      241 gaaagcgttg tcttttgttt ctctatcact tatagtgatg acgtcatatt aaaacacaag
      301 gaggaaatca atggatacag ctgcaattta tcatcgaccg gaaagtgaat atgcatttct
      361 atatacgaat acaagatttc gtattcggct tcgtactagg aaaaatgata tcaaaaaagt
      421 atatgtgctt tgtggagatc catataccat aacaactgaa aaatggtatc aaaaacaacg
      481 accaatgaaa aaatgtctaa gtaccaatgt tcatgattat tgggaaattg aagtgacgag
      541 cgaaacaagg cgcttacagt atgcgttcca tgttgttggg gaagataaca cggactgttt
      601 ttacggtgat caaggaattt ttccttatcg agaaaatgtg attactgaac caaattttta
      661 ttttcgaata ccttattttc atcaaatcga tcgattcact attccagaat gggtaaaaga
      721 aacagtatgg tatcaaattt ttcccgaaag atttgcaaac ggggacaaag caaatgaccc
      781 cgaacacact ttaccttggg gaagtaaaga tccagaccga gaagacttct tcgggggaga
      841 tttgcaaggt gtgagggatc atttggatta cttgacggac cttggcgtca atggcatcta
      901 tttttgtccc atatttaaag ccacatccaa tcataaatac gatacgattg attattatga
      961 ggtggatccc gcttttggcg ataaaaaact tttaaaaaac ttgattgatg aagcacataa
     1021 aagagggata cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc attcaccaca
     1081 gtggcaagac gttctcaaaa acggagaaaa atcgatctat aaagactggt tccacatccg
     1141 ttttttccca gtagattctt atcagatgac tgacttacca gaaactgctg aaaatatccc
     1201 atatgatact tttgctttta ctccattcat gccgaaacta aatacagcaa atccagaagt
     1261 gcaaaaatat ttgctggata tcgcaactta ttggataaaa gaattcgata ttgatggatg
     1321 gcgtcttgat gttgctaatg aagtcgatca tcatttttgg aaaaaattca gagaagcagt
     1381 tacagaaatc aaaccagaca tctatatcct tggagagatt tggcattcct ctcaagcttg
     1441 gctgcaaggg gatgaattcc atgcagtgat gaattatgca ttcacagatt cgattaaaga
     1501 ttattttgca aaaaagaaaa tcacagcttc acaaatggtc agtggaatga atcatcagca
     1561 aatgctttac agagatcagg tgaacgaagg aacattcaac ttattagatt ctcatgatac
     1621 tgccaggatc ttgacccttt gccaaggtaa caaagaattg atgaagtctg tgatggcctt
     1681 tatgtttctt caaaaagggt ctccttgtat ctattacggg acagaaatag ggatgactgg
     1741 tgaagatgac ccggattgcc gcaaatgtat gatatgggag aaggaggaac aagatctaga
     1801 gctattcggt tttataaaag aacttgtttc tctaaggaaa cagttgtcta agattatttc
     1861 tgaaggttct actcagtggc taatagtata tgatcgtgaa gataagctct atttcacacg
     1921 ggaactagag ggacagatca tctatgttta ctttaatcag tcaaaagaac cttgggtagt
     1981 agaacaggaa aatgaggtga ttcttagtca aaattgtcag ttgttagaag acgggaaagc
     2041 agagatacga caatacggat ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag
     2101 aaagcgtttg caaatgaaga tttgtgccca ttataataag ggtgtaacag cacgttgaat
     2161 acacaggagg aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt agggttaatc
     2221 tctttaggaa tcttaggcgg attagcggct tgtggcaatg gtaactcttc agcagaaggg
     2281 gaagccgatg aaggaaaaac gttaacagtt tccgttgatt ctggatataa agattatata
     2341 aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag
     2401 gacatgttcg atcagttgga atcattagcg ttagatgggc ctgctggaaa gggacctgac
     2461 gttatgatgg cagcatatga ccggatcggt gcgttaggac agcaaggaca tctggcggaa
     2521 gtaaaactgg gaaatgaagc agcttacgat gaaacagaca aagcccaagt gacttatgat
     2581 ggaaaaatct acggagaacc tgcagtaatc gagacccttg ttttgtacta taacaaagat
     2641 ttagtcgata cagcaccatc aacgtttaaa gatttggaga atctatccaa agacagtcgt
     2701 tttgcttttg agtcagaagc aggaaaaaat acaggatttt tagctaaatg gactgacttt
     2761 tattattcct atggtttaat tgcaggttac ggtggctatg tatttggtga tgatggcaca
     2821 aatccttcag atatcggttt gaataatgca ggtgctgtag aaggaatctc ttatgcaaca
     2881 gattggttca aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc aggagacttc
     2941 gccagccagc aattcatgtc caataaaact gcagcaatca ttgacggtcc atggcaagcg
     3001 caaacataca aagaaaacaa tatcaattat ggtgtagcca aaattcctac tctaaataat
     3061 ggacagcctt atcaaccgtt tggcggagga aaaggatggg tagtcagcaa ttatgctaaa
     3121 aacaaagacc tctctcaaaa atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa
     3181 ttctttgaaa tggtaaatga aatccctgca aaccaacaag ctagggaaac agcaaaggct
     3241 aaaaacgatg aattaacaac agctgtgatt gaacaatatg aaacagctca agcgatgcca
     3301 aacatcccag aaatgggcga agtttggaca ggtgcagaaa acttgatgtt cgatgcagca
     3361 tcaggttcaa aaacaccaaa agaatccgca gatgaagcag taaaaacaat cagtgaagcc
     3421 atagaacaaa aatactgatt aaaatgataa ccgtccattt ttataaaaaa tggaacaggt
     3481 gcttcagata aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt tgccattatc
     3541 ttaaaagcag tttgcggttt agcaagttta gagggggcat tcagatgcag caagataaaa
     3601 caagcaatat caacgaaaaa aaaatcacgc tttgttcact tattccaggg atggggcagt
     3661 ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc catattcgtc ctattcattg
     3721 gtcagatgct tctgggcgga tttcatgcat tggtaggatt ggttactttg ggaagtatac
     3781 caatggaaga tcattcatta tttattttga ttgaaggcac tttgcaattc atcattacaa
     3841 tcattttctt gattttttac ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc
     3901 gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt catacataat ataggtgatg
     3961 aaggttttcc ttatctattg acacttcccg cttatcttct catgattttt acgatcattt
     4021 tcccagtcct agttacattg ttcactgcat ttactaatta tgacttcaat catattccac
     4081 cagccagttt aattgattgg gtaggggcta aaaacttttt tagtatcttc tttttaagct
     4141 catatcgtaa tacttttttt gcagtctttt cgtggaccgt gatttggact ttatgtgcaa
     4201 caactttgca aatcaccctt ggtattctca cagcggttgt tgctcaccag aaatttatta
     4261 aattcaaacg tattttcggt atcattttct tattaccatg ggcggtccca gcttttatta
     4321 ctatcatgag tttttcgaat atattcaacg atagcgtagg tgccatcaat actcaagtga
     4381 ttcctttcat aaaccagctt ccttttgtag atatcagtgc aatctcttgg aaaacggacc
     4441 cgaactggac gaaagtagca attatcttag tgcagggctg gttaggattt ccctatatct
     4501 atgtgatggt tacaggaatc ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa
     4561 tcgacggagc aaatgcatgg cagcgctttt caaacatcac gcttcctgct gtcttcctag
     4621 tggcagcgcc aacatttatt acacagtata cggggaattt caataatttc tccatgatct
     4681 atctgtttaa caatggtggt cccggaagcg taggcggagg agctggttct actgatattc
     4741 ttatttcctg gatctataaa cttacgactg gaacttctcc gcagtattct atggcttcag
     4801 caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga
     4861 caaatgcgtt caatatgggg gattaacaat gaaaaaattt cattcgagtg ctcgattcca
     4921 aaaacgactt tctcactttt ttacttactt ttttctttta atactgtcag tcattatcat
     4981 ctatccgctt ttgatcacag caagtactgc cttcaagtca ggaaatattg cagcttttag
     5041 tttagatctt tcaagtgact ggactttagg aaactttact cggctattca gtgaaacact
     5101 ttacgggact tggtataaaa atacattgat catttctgtc ctaacaatga tcgtacaagt
     5161 gactgtagta actttggcgg gctatactta tagtcgctat cgttttatcg gccgcaaatc
     5221 aagtttgatg ttctttttag tgattcagat ggtaccgact atggcagcat tgactgcctt
     5281 ttatgtcatg gctttcttat tagaagcgct cgataagtac tggttcttga caatgatcta
     5341 tataggcggt ggaattccaa tgaatacttg gctgatgaaa ggatactttg acactgtacc
     5401 aatagattta gatgaatctg ctaaacttga tggggcaggg catttcagga tttttttcca
     5461 gattgtatta ccgcttgtca aaccgatgct cgccgttcaa gcattatggg cttttatggg
     5521 accttttggg gatttcatgt tagctaagtt cctgcttcga acacctgaaa atcagactgt
     5581 tgctgtcggc ttacaaacat ttatttctga tgtgacaaac caaagagtct ctctttttgc
     5641 tgctggggca attttgattg cactaccgat ttgtctatta ttcttctttt tacaaaagaa
     5701 ctttgttacc ggcctgacat ctggcggaac aaaaggatag gagcagagta tcatgcaaac
     5761 atttccgtta aactattttt ccgcattacg tacgccaact caagtgtttg cgggacgcaa
     5821 actattatca tggcctaaat ttttcttgat atttgtattt ttagtttcct tgatggtcat
     5881 gcctgtgact cttttttatg ccaatcagat ccaagcgatt ccattagaac aatttctatc
     5941 cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg aattatctga
     6001 aactgggtta caagcatcgc aacaaacgat agctgtgacc cctgaaattt tggtaggtgt
     6061 gtctttatct gagaaacaac aatccgatca tggaactttt attgattttg agaaggaaca
     6121 atgggtgatc caacaaaagg ataaatctgg tatacgccgc tatacgatga attatagccc
     6181 atctttccaa ccagattctg tacgtacgcc cgaagatttc caacgatttt tagaaagaga
     6241 attttatgcc agtaatcgtc caacaatcat tttatcttat agtttaagtc ttggtcttgt
     6301 cctatttgtc atgacgagtc tcattttatt cggggcttcc tttttcttgt ggatgacaag
     6361 aaaaagccaa ctttcatcca ttcatacatt caaagagagt gcaaatttga tgctgaatgt
     6421 tatgggaata ggttccatgg cagcagcagt tgtagggttt atccactttg actttatctt
     6481 gatgctaagt gtccaaacaa cgataactgt cttgctgctt ttatgggttt ttgtcaaaac
     6541 aagattcaaa gataagcgtg tcgaatgaca agtttaagcg atttatgcga caatgaatgt
     6601 gaaaaaaatg aaaaataaag tagtttttga aaaatttctg aaaaaattaa acttcttagt
     6661 tgattctttt cttttttagt attaacgtaa tagggtagtt tttttaagga ggaaaatcat
     6721 gttagagaaa actttataca atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt
     6781 aaattattgg gacggtacaa cgaaacaata cggagatacg gacactcctc cccaaatcaa
     6841 aatcaatatc catgaagaaa tcccaatgaa agagattaca agtaacgctt ctttggcact
     6901 aggagaagcc tatatggatc atcgaatcga gatcgaggga agtatccaag cattgatcaa
     6961 cgatgcttat cagcaagcaa acagcttttt acgaggaaaa gattatctaa aatggctgcc
     7021 aaagaaagaa aaacatacga aacaaaaaaa caaagaagat atccatgcgc attatgactt
     7081 aggtaacgac ttttataaac tttggctaga tccaactttg acttattctt gtgcttattt
     7141 caatacacct gaagatacgt tggaacaagc acaaatcaat aaagtccatc atatcttaga
     7201 caaactcttt atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt
     7261 gatgttcact gctaccaaag agtataatgt caaggcaact gggatcacac taagtgaaga
     7321 acaatacgat ttgattacca aaagaataaa agaagaacat ttagaagata aatgccgtgt
     7381 attattaatg gattaccgcg aactaaaagg cgaaacgttt gatcatatca caagtgtagg
     7441 tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt
     7501 attgaaacca aaaggaacag ctttgatcca tggaatcagc cgacaacaag gcggtgcgac
     7561 aaatgcttgg atcaataaat atattttccc tggcggttat gtccctggtg ttactgaatt
     7621 agttgataat attacaaaaa acgatttgca actgattgat ttggaaagct tacgtcgtga
     7681 ctaccaattg acattagaac attggacaaa gaattttcat caagtccaag atcaagtcac
     7741 gaaagaaatg ggtgattcct tctatcgaat gtgggatcta tatttacaag cttgtgcagc
     7801 ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa
     7861 tgacatccca atgcatcgtg cataattgag gcaaaagtac tgtttaaaga ctatttaaaa
     7921 agaagctaag ttttatgatg ctccagaagt gaactttttt gtctaacttc taggattgtc
     7981 tctaaagctt agtttctttt tctttatatt ttcataataa aagtttggtt ctttaatttt
     8041 taaacatgac agaaaatttt ttcttc
//
LOCUS       DAZKMB010000116         8023 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000116 DAZKMB010000000
VERSION     DAZKMB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.118"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..1797
                     /locus_tag="ACG8V3_002377"
     CDS             259..1797
                     /locus_tag="ACG8V3_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0300856.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1794..2759
                     /locus_tag="ACG8V3_002378"
     CDS             1794..2759
                     /locus_tag="ACG8V3_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300857.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2746..4068
                     /locus_tag="ACG8V3_002379"
     CDS             2746..4068
                     /locus_tag="ACG8V3_002379"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0300858.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4131..4235
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4309..5052
                     /locus_tag="ACG8V3_002380"
     CDS             4309..5052
                     /locus_tag="ACG8V3_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0300859.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5067..6059
                     /locus_tag="ACG8V3_002381"
     CDS             5067..6059
                     /locus_tag="ACG8V3_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0300860.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6071..6808
                     /locus_tag="ACG8V3_002382"
     CDS             6071..6808
                     /locus_tag="ACG8V3_002382"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0300861.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6798..7520
                     /locus_tag="ACG8V3_002383"
     CDS             6798..7520
                     /locus_tag="ACG8V3_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0300862.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7604..>8023)
                     /locus_tag="ACG8V3_002384"
     CDS             complement(7604..>8023)
                     /locus_tag="ACG8V3_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0300863.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2570 a         1389 c         1751 g         2313 t
ORIGIN      
        1 aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag
       61 atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct
      121 tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt
      181 aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat
      241 aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag
      301 ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca
      361 ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta
      421 ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc
      481 ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt
      541 ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca
      601 gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca
      661 ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact
      721 ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga
      781 aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc
      841 agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca
      901 gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa
      961 accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa
     1021 gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg
     1081 agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt
     1141 actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa
     1201 gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta
     1261 aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa
     1321 catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc
     1381 acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg
     1441 aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga
     1501 gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg
     1561 actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa
     1621 gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata
     1681 acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca
     1741 gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta
     1801 aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc
     1861 taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa
     1921 aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact
     1981 tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg
     2041 tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc
     2101 ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa
     2161 aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag
     2221 aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg
     2281 acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt
     2341 ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa
     2401 tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac
     2461 cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga
     2521 aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa
     2581 taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc
     2641 ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga
     2701 caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat
     2761 gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca
     2821 ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga
     2881 tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg
     2941 agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa
     3001 cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc
     3061 gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa
     3121 gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg
     3181 gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa
     3241 gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct
     3301 gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg
     3361 tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt
     3421 gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg
     3481 gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta
     3541 gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg
     3601 caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa
     3661 ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca
     3721 agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct
     3781 gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt
     3841 accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt
     3901 gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc
     3961 gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc
     4021 atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa
     4081 ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc
     4141 tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag
     4201 ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc
     4261 atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat
     4321 ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt
     4381 tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca
     4441 gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg
     4501 gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta
     4561 gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg
     4621 ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg
     4681 ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag
     4741 caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa
     4801 ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc
     4861 agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata
     4921 cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta
     4981 ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt
     5041 tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt
     5101 tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt
     5161 tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc
     5221 tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag
     5281 gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt
     5341 atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg
     5401 ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt aacatccatt
     5461 caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg
     5521 tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa
     5581 gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag
     5641 gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag
     5701 aattagatga acggttagat tactatactc cggtaattat tacgaatgat caactgatca
     5761 aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag
     5821 ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc
     5881 ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga
     5941 attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg
     6001 gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg
     6061 agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat
     6121 attagaagac atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag
     6181 cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg
     6241 aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc
     6301 gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa
     6361 aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt
     6421 aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc
     6481 cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac catttggagc
     6541 gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc
     6601 aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag
     6661 gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa
     6721 acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt
     6781 attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt
     6841 tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag
     6901 aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc
     6961 atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa
     7021 ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa
     7081 aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc
     7141 acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt
     7201 tgatccattg gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt
     7261 tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc
     7321 caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg
     7381 ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg
     7441 agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt
     7501 tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg
     7561 ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg
     7621 cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg
     7681 ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt
     7741 aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga
     7801 tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag
     7861 caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa
     7921 caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt
     7981 gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAZKMB010000117         7969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000117 DAZKMB010000000
VERSION     DAZKMB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.119"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..919
                     /locus_tag="ACG8V3_002385"
     CDS             92..919
                     /locus_tag="ACG8V3_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300864.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(1192..1962)
                     /locus_tag="ACG8V3_002386"
     CDS             complement(1192..1962)
                     /locus_tag="ACG8V3_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0300865.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            2180..3247
                     /gene="ulaG"
                     /locus_tag="ACG8V3_002387"
     CDS             2180..3247
                     /gene="ulaG"
                     /locus_tag="ACG8V3_002387"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0300866.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            3265..3726
                     /locus_tag="ACG8V3_002388"
     CDS             3265..3726
                     /locus_tag="ACG8V3_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0300867.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            3746..5230
                     /locus_tag="ACG8V3_002389"
     CDS             3746..5230
                     /locus_tag="ACG8V3_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0300868.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            5273..5572
                     /locus_tag="ACG8V3_002390"
     CDS             5273..5572
                     /locus_tag="ACG8V3_002390"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0300869.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            5587..6225
                     /locus_tag="ACG8V3_002391"
     CDS             5587..6225
                     /locus_tag="ACG8V3_002391"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0300870.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            6230..7090
                     /locus_tag="ACG8V3_002392"
     CDS             6230..7090
                     /locus_tag="ACG8V3_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0300871.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            7080..7790
                     /locus_tag="ACG8V3_002393"
     CDS             7080..7790
                     /locus_tag="ACG8V3_002393"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0300872.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
BASE COUNT         2603 a         1258 c         1706 g         2402 t
ORIGIN      
        1 ttattctgtt ctaaacattt ttggttgttt agaacttttt tttgtgtatg atataaagga
       61 gaaataattt tttaaaggga aggaaattgt tttgattaaa gtagatggag cattactaaa
      121 agaataccga aagaaaaagg ggttaacaca agtacaatta gcagaaggca tttgtgaaca
      181 agcaactgtc agcaatatcg aaaacaagaa tatgtcgcca ggcatcaata tcttaagtgc
      241 gctttgcaaa aagctgaatg tcgagatctc cgatatatta gtagaaacag aagaagacaa
      301 aatcaggaaa aaaataaatg agattcaatc attgaattct gtatcaaaat accaagaatc
      361 ttataaaatt ttacaaacga tcgatcagga aaaagtaagt aattctgatc ttttaaatca
      421 aattttattt tatcaaggac tgcttgatca tctttatgcg aaaaaaccag atgcagcatt
      481 attttatttt aatcaagtgt tggaacaaac gaccgaaaca gatatccatc acttacaagc
      541 agctgcaaac gtggcaatga tctacttgtc aaaaggagaa ttagattttg cgaaagttta
      601 tgtggagcgt acacttaaaa tattgagtga gactgatttt gataatctga tggtctgcat
      661 tgtatattat gatatcgcca cctattaccg taaaatcgag gattatgata aagcaatcca
      721 attatgtgag aagggtattg agtataataa gaaacataaa tcgacttatg cattggaata
      781 cttgctttat gaaattgctt cttgccataa gcaattagga gaagatgatt atttagaaag
      841 atatatggac gcaaaaaaaa tagcgcgttt caacggaaat gattacgcgg taaaggtgat
      901 tgaaaacgac ctgaaataaa gtggttgtga aatcagtgtt ctgatttgaa taccgtttat
      961 tcgaaaaaag gaggtgtgac ttttgtcata cctccttttt tcgatattgt ttgattccta
     1021 tttaggcaac atataacggt tataaaagaa gcaatagatt atgatggtat cgattggatg
     1081 gatacatctt gggattcctt tatcaatttg agcgtagcgt gtttttcatt tatcgataaa
     1141 tttgttattc atttttttga taatcctatt tacatattta cgtcctggtt cttaaaatga
     1201 atctacttga atggttttta tccccgtatt tctgatctcc tctaatactt tttcagaaca
     1261 aaatttatca gtgatcaaat aatcaaagac tgaaatatca gcaatcttga actttgatgt
     1321 cagtccgatt tttcgatgat cagcgactaa aatcttacat ttgattgtct tgttgaccat
     1381 catttcgttg atcgttgatt catttaatat ctttgttgtg accccatgta ctacatcgac
     1441 tccgctacag ccaatgatac aaatgtctgc attgactgaa gaaatcgtat ttaatgcgat
     1501 gtcgcccact agtgcttctt ttggaaagcg catctctcca ccagttaaaa tataactaca
     1561 attatctttg tgcggagagt aattgatttt cacgttgttt gtaatgactg tcaaatggtt
     1621 agattgaaga aaatccagtg ttttcaacgc agtcgagcta gaattgataa acagcgtatc
     1681 ataatcgtta atgtatttac tagcttctct ggcaatcgct tgtttaatcc tttcaggacc
     1741 agattgatca aaatcagtct ggtttttgta attataagaa caataaccgt aattgcgtat
     1801 gatctcgttt ttttcttcta ataaatttaa atctcttcgt atcgtactaa tagatacatc
     1861 aagggattca gctaattcga aagttgatag ttgttcagtc tgctgaagca gttccaaaat
     1921 ttctttttga cgtttttcaa tattgacaag tgagtttttc atgacttttg cctcctgagg
     1981 gacatcttga tccatggatt tatttgacta gttgttcatt taaacattca tatctgtctt
     2041 tattatactg aaacaatcat tgattactga aagaaaatca tataaaaaac aaacggtcat
     2101 ataaaagggt tttcatggat gctgatacac attaatattt acttgtaagt tggaacaacg
     2161 atatgggggg agcaagcaaa tgagtaaaac tgtcaatgaa gtaacaaaag aaaagtggtt
     2221 agtagaaaca tttcctgagt ggggcacata cttgaatgaa gaaatcgaaa cagaagtagt
     2281 ggatcctgga acttttgcta tgtggtggct gggctgtacg ggaatttggc tgaaaagtca
     2341 tgaaggaacg aatattttat gtgatctatg gtgtgggacg ggaaaacgta gtcatgggaa
     2401 tggcatgatg aaaaaaggcc atcagatgat gcgtatgagt ggcgttcaaa atatgcagcc
     2461 taatttacgt actcagcctt tcgtaatcga tccttttgcg ataaaaaatg tagacgcttt
     2521 aatcgttaca catatccatt ccgatcatct agatatccat acagccgcag cagttcatca
     2581 aaattgtcct gaagcaacat tcattgggcc aaaagaagtg gttcgcacgt ggcaaaaatg
     2641 gggaattcct gaagaaaaaa cgattacagt aaagccaggt gatgcagtca aaatcaaaga
     2701 catcaatgta gtagctttag aagcgtttga tcggacagcg ttggtcactt gtgaagatcc
     2761 agaagaggta ttaaaaggaa aaatgcctca ggagatggat caaattgcag taaactattt
     2821 attcgaaaca tcaggaggga atctttatca tgcaggagat tcacattact ccaatttatt
     2881 tgccaaacat ggtaatgaac acaaaatcga tgtctgttta ggggcctatg gtgaaaaccc
     2941 acgagggata acagacaaag ttacttctgt tgatatgctg cgcatggcgg agtcattgaa
     3001 tgcaaaagta gtgattcctg tccactacga tatttggtct aattttatgg ctgatccaaa
     3061 agagatcact gaaatttgga aattcaaaaa ggatcgcctg aagtatggat ttaaacctta
     3121 tatctggcaa gtaggcggga aattcatcta tccgacaaac aaagatgatc ttgagtttaa
     3181 tttttatcga ggatttgaag atgtcttctc gattgaaaat gatacaccgt ttccgtcgtt
     3241 tttataagaa agaaggggag tttcttgctg aaatactttt ttgaccatca attgattcat
     3301 tacccagata aagaaatcac ggattggcgt gaagctatcg ccgaaagctg tcttgtattg
     3361 atcgataagg aaattattga tgaaacgtat gtggaggaga tcatccattg tgtagaaaaa
     3421 tacggtcctt atattgtgat cgcacctcaa gtagcaatgc cgcattcttc agaaaaaagt
     3481 gatggtgttt ttggaacagc tatttcattt accaagttga aacagccaat ccgttttgaa
     3541 agtgaagggg agaatgagga gaaaacggct agtttgtttt ttacactcgc agcaaaagat
     3601 ccagaagaac atctagaaaa tatccaaaaa ttatccgaat tattaatgac agatggattg
     3661 gttgagcgtc tcatctctac gaactccatc gatgattacc aagttgtgat ggcagaatat
     3721 aattaattga tagaggagaa aaaagatggg ggaatttttg ttaagcattt ggacgtattt
     3781 tgcaacaaat atcttgacgc agccagcata cctgattggt tttatcgtgt tattaggcta
     3841 tattttgctg aaacgcccgc ttcatgaatg catcgctgga ttcttaaagg caacggtcgg
     3901 ttactttatt ttgactgttg gctctggtgg tttggtcaat aattttcggc caatcttggt
     3961 tggactaaag gaacggttca atttggatgc catggttaca gatccttatt ttggacaaaa
     4021 tgctgtggat gctggattaa tggagacatt tggccgtacc tttggagatg tgatgatttt
     4081 attactgatt gcatttgttt taaatatctt attagtccgt tttcagaaat atacgaaact
     4141 gcgttccgtt ttcactaccg gaaacgttca ggttcaacaa gcagctaccg ctttttggat
     4201 tttactgttt tgctttcctg atctaggtag gatacaagca ttgattttta tgggactgat
     4261 tttaggttgt tattggacag tagcttctaa cttaacagta ggaatcacgc aggaattgac
     4321 agaaggtgcc ggatttgcag tggcacatca gcaaatgttc ggaatcttta tatttgcaaa
     4381 attagctgag tggatgaaaa agcgggatga aaagaaaaat agatcaatca agcaagacaa
     4441 aaaaattgaa gatatcaaat taccaggatt cttgtcgatt ttcaatgaga atatggttgc
     4501 tacttcgtta ctcatgctat ttttctttgg gatcattctg attgtactag gcaaagatta
     4561 tttgattcaa gcgcaattca tgcaagaagg gcagagtttc ttattctaca ttatgacgac
     4621 ttctttaaat tttgcggtat atctggcgat cttgcaatta ggtgttcgga cattcgtcga
     4681 tgaactaact caatctttcc aaggaatctc aaacacgatt ttaccaggag ctgttccagg
     4741 aattgacgta gctgctactt ttggctttgg ttcgccaaat gcggtaacga ttggggtctt
     4801 gtttggtgct ttgggacagt ttttgatgat tggtttactg attttattga aatcaccaac
     4861 gatcgtcata gccggtttca ttccgttgtt ctttgataat gccgttatag cagtttatgc
     4921 caataaccga ggtggattca aagccgcttg cctgttcccg tttctttcag gaatcataca
     4981 agtaggcggt tcagcattat tcgctacatg gattgggatg tcaacatacg gtggttatat
     5041 cgggatgttc gactgggcaa cagcttggcc attgtttaca gtaatcatga aagtcctagg
     5101 ttatgctgga attgccgtag ttgtcattgg tttattggct attccgcagt tacaatatcg
     5161 gaaaaatcct gatggttatt ttttgattgt agaagactat gacacatatg cagaaaagca
     5221 gagagtataa aagaaaatct gattagacaa cactcataat ggagggatta agatgagaat
     5281 tttagtatcg tgtgcaaatg gatcaggaac aagcttgatg atgatgagaa gtgtggaaaa
     5341 agcaatgaaa gaactaaacg taccaattac caaaatccat cattgtgcca tttcagaggg
     5401 aaaaagttca gctggccaat acgatgtagt ttttacgcca gtcaattttt tgaatatgtt
     5461 tactcaggca gagaaaaaag gagttacagt tatcggtatc aaaaatgtga tgtcagtcaa
     5521 agaaatcagt gaaaaatttg ctgaaacaaa gtggtaccaa aaaaacaagt aaaggaagga
     5581 actaagatga gcaggccgaa tttgcaaatt gcgttagatc acaatgactt agaacatgca
     5641 ttaggagatg ttatgaaagt tggtgatgta gtagatatta ttgaagtggg cacaattctt
     5701 tgcttacagg aaggacaaaa agcgattcgc tgtattcgca gtatgttccc agataaaaaa
     5761 ctagtcgctg atacgaaatg tgcagacgct ggaggaacag tggcgagtaa tgttgcaaaa
     5821 gcaggagcag actggatgac agtcatttgt tgtgcaacga ttccaactat ggaggcagca
     5881 caaaaagaaa taggtgaatt gcaagtagaa ctctacggca attggacttt tgaacaagcc
     5941 atggattggc ataacatcgg tatccggcag gtaatctacc atcaaagccg tgatgcgctg
     6001 ttatctggtg aaacttgggg tgaaaaagat ttatcgaaga tcaaaaaact gattgaacta
     6061 ggctttaatg tttctgtaac tggtggatta aatccacaca cattacactt atttgaaggg
     6121 atcgatgtgt atacctttat cactggtcgt ggtattacag cagcaaatga tccgatgaag
     6181 gccgcacaaa acttcaaaga tgagatcatt cgtatctggg ggtaaggaaa tggctcaaat
     6241 tgggatttat gaaaaagcgt tacccaaaga tatttcatgg aaagaacgtt tttcccttgt
     6301 aaaagaaatg gacttcgatt ttatcgagat gtcgattgat gagacggatg agcggttagc
     6361 acgattagat tggtctgaag aaaagatagc tgagctgaga gaagagatgt tcagctccgg
     6421 tgtacgtatc cattcgattt gtttaagtgc gcatcggcga tttccgtttg gttctgctga
     6481 tccagaaaag aaaaaagcag cgaaacaact aatgaaaaaa gcaatccatt tggcgcataa
     6541 tctaagcgtc aaagtaatcc aaatagctgg atacgatgtt tattatgaag agaaatcaat
     6601 gaattccaga gaagacttta tcgaaggaat aaaagaaagc gtaaaggaag cttctaaata
     6661 tggcatcatt ctttccgtgg aaatcatgga tgatccattt atgaactcca tttcaaaatt
     6721 tttagaaatc aaagaacaga ttcattcacc ttttctacaa gtatatccag acctaggcaa
     6781 tttgtccgca tggccggaaa ataatccggc tcgagaactt gagaaaggga tcgattatat
     6841 cactgctatc cacttaaaag atacttaccc tgtaacaaaa gaatattcag gccagtttcg
     6901 tgatgttcct tttggtagcg gttgtgttga ttttcttgga ttattaagac atttgaaacg
     6961 tctagattat gatggaacat ttctgattga aatgtggagt gaaaaaagcc aagactttcg
     7021 aagagaaatc cagcaggcaa aagactactt aagttctaaa ttgaaggagg ctggctatga
     7081 tgtcttgtga agaaaaaatt gcagcaatga agcaacgtgt ccttgaagca aatctggcat
     7141 tgccgcgata cggattagtc aaattgactt ggggcaatgt gagtgaagtg gaccgtgagc
     7201 taggagtcat cgtcattaag ccaagcggtg tcagatatga atgcatgcaa gcagatcaaa
     7261 tggtggtaac agatttgtcg ggaaatataa tagaagaaga tagcttaaaa ccatcttcag
     7321 atctccccac acatgttgtt ttgtatcaaa cttttgaaga tataactgct attactcata
     7381 ctcattccac gcactccgtc atgtgggcgc aagcaggacg cgatttgcca gcttatggca
     7441 cgactcatgc tgatgctttt tatggtaagg ttccttgtac acgccaatta acgaaagaag
     7501 aagtaagaga agcttacgaa gttcatacag ggaatgtgat tgtcgagacg tttaaggaaa
     7561 gaaaattaga tccaaacgaa gtccctggag tacttgtcta tggacatgga ccgttcacat
     7621 ggggggattc acctatgaaa gcagtagaaa acagtttgat cttagatgaa atttgtctga
     7681 tggccaaaga aaacgagctg atcaacccca atatatgtga aatcccgcaa tatttactag
     7741 ataaacatta ttttcgcaaa catggaaagt atgcatacta cgggcaatag agatgctagg
     7801 gtaagggtag aaaattaaaa gataacactt gatagagaga aacagaggtt gtgaagaaaa
     7861 tttatcacaa cctttttatt tatcaaaaag tatgcattct aaaaggaaag aggaatttta
     7921 aggaatcgag agaatcttta caggagagtt atatgtgata gattaaata
//
LOCUS       DAZKMB010000118         7820 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000118 DAZKMB010000000
VERSION     DAZKMB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.121"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..768
                     /locus_tag="ACG8V3_002394"
     CDS             67..768
                     /locus_tag="ACG8V3_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0300873.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1377..2393)
                     /gene="tsaD"
                     /locus_tag="ACG8V3_002395"
     CDS             complement(1377..2393)
                     /gene="tsaD"
                     /locus_tag="ACG8V3_002395"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0300874.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2390..2854)
                     /gene="rimI"
                     /locus_tag="ACG8V3_002396"
     CDS             complement(2390..2854)
                     /gene="rimI"
                     /locus_tag="ACG8V3_002396"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0300875.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2861..3403)
                     /gene="rimI"
                     /locus_tag="ACG8V3_002397"
     CDS             complement(2861..3403)
                     /gene="rimI"
                     /locus_tag="ACG8V3_002397"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0300876.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3387..4109)
                     /gene="tsaB"
                     /locus_tag="ACG8V3_002398"
     CDS             complement(3387..4109)
                     /gene="tsaB"
                     /locus_tag="ACG8V3_002398"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0300877.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4300..5280)
                     /locus_tag="ACG8V3_002399"
     CDS             complement(4300..5280)
                     /locus_tag="ACG8V3_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0300878.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5304..6788)
                     /gene="galT"
                     /locus_tag="ACG8V3_002400"
     CDS             complement(5304..6788)
                     /gene="galT"
                     /locus_tag="ACG8V3_002400"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0300879.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6800..7789)
                     /gene="galE"
                     /locus_tag="ACG8V3_002401"
     CDS             complement(6800..7789)
                     /gene="galE"
                     /locus_tag="ACG8V3_002401"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0300880.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2188 a         1693 c         1388 g         2551 t
ORIGIN      
        1 aaactttaca aattctttaa aaaaagtgac aataaatata gtgaacaata aggagaggac
       61 tttttaatgc gtgttaataa aatcgatttt cacttttcaa aactcagata tcaacctgat
      121 tttgaacatt tctcggatga agagttccaa cagatcaagg aacatactgt tcttcgttct
      181 tataaaaaag gacagatcct tttcgacgat ggtgatccca gacaacggtt gtatatcttg
      241 accaaaggac tcgttcgttt agaacgttat gatgaaagtg caacttatta ctactatgac
      301 tatgtgaata caaataacct ttttcctatg tcaggaatgt ttggagaaga gacctacact
      361 tatacagctc aagcgatgac tgatattgaa acattctacc ttcctgtttc ctactatgaa
      421 aaattagcac gaaaaaatcc cgaacagttg atctatatga tacaaaaaat ttctaaagta
      481 gttgaaatac atgaattgcg tatccaaatc ggattgacct ctagcgcctt tgaccgggta
      541 aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc
      601 accattcctt atccgatcac cttaaaagaa ttagcagtga atagcggcac gacacgtgaa
      661 actgctggac aagtaatcaa acaactacgt gattccgggc tgttagatta tgacaaaaag
      721 cgttttactt tctgtaagac agctactact ccagctatga gagaataacg cgaacaaaaa
      781 taagcagtga ccctgataaa tatcagggtc actgcttatt ttcctcccta aaacttctga
      841 aagatccggc agttttagat cactggacaa gactcaaaga ttttctatgt gtgtttcagt
      901 tatgtaatat tcactgtttg caaaaaatac tgttagatta actagatgaa gcgtcttgtc
      961 ctcatccgat ctagattatt cgatattcct cgacaataaa cgattactca gtgagggcaa
     1021 tcagaggaac ggataatgat aaataagctg taaaaaatag caaaataaca ataatgatta
     1081 aaagtatgta aaaaataggg ctttgacaaa aacgtgtcac agccctattt cgattaaacg
     1141 gtgtgtacct gtcaactctt cggtcacaag tcataatgtt cataaacatg aggagctgtt
     1201 ttttgaaatt gttccttgtg aatcagagtt aaacgacagg cttacgaact cttgattaaa
     1261 cggcgttcaa aagcttgcat acgcggtaat aagaacagcc aggtcaaaaa catgagaagc
     1321 cgtttttcct gggctgttct tattacttga tgcagggcag ctttttcaca acctctttat
     1381 tcagataaaa ccaagcttgg atcagcattc aactgatagc tagcaaaatt tcttttttgc
     1441 atttccacat gtgctgctgc tccaatcatt gcagcattat ctccacaaag tcgaagaggg
     1501 ggaatcacta gttctacctc agggagttta gttgccaaag catcctgcaa tccttcacgc
     1561 aacccttgat tggccgctac acctccagct ataatcagtt gcttgactgg ataattttga
     1621 caagctcgaa gtgttttagt aatcaataca tcgatcacac ttgcttgaaa gctagctgct
     1681 aaatcatttt tgtctagatc ttctccacgc tgttgtgcat tatgcaccaa attgataaat
     1741 gcacttttta atccgctaaa actgaaatca taattatctt catggatcat cgctcgcgga
     1801 aaatggtaat tatcttttcc ctgatgagct agctgatcga tttcttttcc gcttgggtaa
     1861 gaaagaccta atactcttcc aactttgtca taagcttctc ctgctgcgtc atctcttgtc
     1921 tccccaatga tttcatatga tccatcttct tgcatataga ctaattctgt atgtcctccg
     1981 ctaacaagaa gtgccatcaa tggaaactgg aatggtttaa ccagacgggc tgcatagata
     2041 tgccctgcca tatggtttac aggaataagc ggcaattggt gtgcccaagc aaaagcctta
     2101 gctgcagaaa taccaatcag caacgagccg acaagtcctg gtccataagt gacagcgact
     2161 gccgataaat cttctgccga cacaccagct tctaccagtg catcttccaa gcataaggtg
     2221 atttgttcta cgtgatggcg gctagctact tcaggtacta cgccaccaaa acgtttatga
     2281 ctgtttattt gagaagcaac gatgttagat aagatctctg ttccatttct aacgaccgct
     2341 acactcgttt catcacaact tgattcaatc gccagaatca attcttcatt catttcatcg
     2401 tctccgtttt taactttctg ctcataatga tcgcatcttc cgtcggattg cgataatatg
     2461 atttccgttt acctaatata cgaaactttt ctgattcata aagttttctt gctgcttcat
     2521 tagatagacg tacttctaaa aaaacatgca gcgaatcttt tatttgttcc gtaatcagta
     2581 attgtaacaa atgccgtgca tgcccttttc cttgttctga aactgcaaca gcaatatttg
     2641 tgatctcgac ttcatctatt actttcgagt atccaataaa gccacagatc tggttatctt
     2701 cagctaaaag cagataatca gagtgaggct gttggatgtc tgccaaaaac tgcattttcg
     2761 tccaaggtga accatgtgga taagcatttt cgctaatcat ccatacgcat tctgccaagt
     2821 acgcttcatc aaaatctttt tttctgcaaa tcaactaatc tcactcattt tctaaaagtt
     2881 ttatcatatc caacgcatct tcttttgttt cagtgtaata tctttttttg atcgttgttg
     2941 cttgaaaccc caatttccga taaagtcttt gtgcatcttg attactcata cgcacttcta
     3001 atgacatcgt ctcacagcga ttcattatag caaagttctc gatttcgtca ataagcagcg
     3061 aaccgattcg tttacgctga tatgccggtc gaacggcaat attcgtgata tggcaatcta
     3121 aaccgaacgc tcttgaaccg ataaaaccaa tcattcttcc attatcaaag atacctagat
     3181 atagatgagg aagcggtgac ttcaattctg atataaaggc actttttgtc catggcaatt
     3241 cgccattata gacttctcgt tcgacatcca gcagctcttt gatatcttct gtagtcaatt
     3301 ctcttagtac gtaacgacga tcgatttgtt cgactgtttt ttctgtataa atcattttat
     3361 cttgaaaaaa gcttctggca tacgctctaa attttttcaa cataactttc gtctccaggt
     3421 gtgtgcgtct ccaaccattt ttcttctgct tctactcgtt ttaaatagcg aggaagaaaa
     3481 gcttgtttct ctgcaactgg ttcaactaaa gcacctaatt gagccacagt tacaccactt
     3541 ggaatatccc atgctggaac tgtattaatc atcgcactag gcaatatttc ctttatttca
     3601 gagaagaatt tgtgacaatc actcccgacg aaataaatag cttcattcag cttttgtagt
     3661 tgtgtgaaca aatcagaagc agaaatatgt acatcttcaa gtacagtttc taatctattt
     3721 tctttccagc ggtatgcccc agcgtataca ttttttcttc gagcatcaaa catgggcaca
     3781 ataatattat ccacaccgat gcaattagcc gcgatggttt tcagactaga tactcctact
     3841 agctctttat ctaatgtata agcaatcgtt tttgctgtcg ttacacctat acgaagccct
     3901 gtataagaac cgggaccatc agaaaccgcg attcggtcaa tgtcttttgg acttatttga
     3961 agatctgcaa acaattggtc gattgcaggc atcagtgtta cactgtggtt gcgtttgata
     4021 tttgtttgta tttgtccaag aatttgttcg tcttctacca caccgatcgc caaagtttgg
     4081 tttgcagtat ctattcctaa tgtaatcata tatttgatcc tttctgctat gacattctcg
     4141 ccttccattg tagcatactt ttttattctg aggatgaaaa taccaaaggc aaaactagta
     4201 caataataca tgattatctg ctggcgggca aattttgccc cttcctgaaa cacaaaaaag
     4261 ctgcggtatc actccatttt cccacagctt ttccatcttt tatttcgttg attctcgaat
     4321 aatcaactct gtttccacaa taatctttct tgaaaccggt gctttggaat tacttaattg
     4381 aagaatcgtc tccactgcta gctctcccat ccattcggta taaactttta cagtagaaag
     4441 cgagggcgtt acatatttcg ctacactgac atcattgaag cccatcacag aaatatcttc
     4501 tggtaccact aaaccttttt cttgaatcgc tttcagtgcg ccaatcgcta atgcatcatt
     4561 agctgcaaaa agcgcatcag gaacatgcgg attcgttttt aaatactgct tcactgcttg
     4621 atatcctgac tcgacagaaa aatctgcttc gataattgga ccttcgtcgt agcctttttc
     4681 tgccaaaact tgttgaaatg ccataattct gggatctttt agccgctgat ggtttttctt
     4741 cgtatattct gttccagcaa gcatagcgat atgttgcgct ttatgggaga caaagtgttc
     4801 aatgactttt tgaacacttg acttaaagtc gacaactaga gagtcgattc cttgagaagt
     4861 gccgtcagaa tctaccaaaa gcaaggcatt ttgggatcct ttcatttcta ataactgagc
     4921 ttcgtcgaat tttcctagtg cgatcgttcc atctgccgga agatcactaa tggaatccca
     4981 cgtttctttc aataaagtaa cgtttgcttc ttctgccttt ttttcgattc ctaggcgaat
     5041 agataaataa taaagatctt caagctcttc cgtatcgtcg taccactgga tcaggcgaag
     5101 cgtttgctta gtatttgctg catttttacg atgtttcgta taatttaagc gttctgctgc
     5161 ttcaaaaatt ttttgctttg tggctaagcc aacggaaaga tcaggatcat agttgagaac
     5221 tcttgaaaca gtagcaggag aaactcctgc caactgcgca atgtctttaa ttgttgccat
     5281 ttgtctctcc tcctttttta cgtttataaa atcgcagtaa atcgttcaaa cgctgcctgt
     5341 cctttttcat ctcgcttgaa tactccagcg tcttccagta cacgtgcaaa aacctgacct
     5401 acagattttt gtacgacatc ggtgaccgta tctttcgtca aatcaggatg ttcttttttt
     5461 aattgttctg cccacggacg atgatattca gccatctggt tttcttctcc taataagaaa
     5521 tgctctactt ctactagttc tggtttcaaa cgaggtggta aaacagcaag ccccattact
     5581 tcgatcaacc caatgttctc ctttttgata tgttggacat ctttatgtgg atgaaaaatc
     5641 ccatcaggat gttcttctga aacattgttg tctcgaagga caaggtccaa ctcaaagtaa
     5701 ttgtcccttc ttcgtgcaat tggcgtaatc gtatggtgtg gtgtaccatc tgctgaaaaa
     5761 gctctgatag atactgtttc atccgaatat gctttccatt tttccaatac tgagttagct
     5821 gcttcaataa gcaagttttt ctctgttgat tgcaaacgga tcacagacat cggccatttc
     5881 acgactcctg cgatgacttg cggataatct tctagtttga taagtttttc tatcggtgct
     5941 tttgccatag gaaattcatg acgtcctgcc tgataatgat catgagaaag aatcgatcct
     6001 ccaacaattg gtaagtcagc attcgaacca acaaagtaat gaggcaagac ttcagtaatc
     6061 cgaagcagtc tagcaaatgt ttcctttgtg attttcatcg ggcggtgttg ttctgataaa
     6121 atgatcgaat gttcattgta ataagcataa ggggaatatt ggaatcccca gctttcaccg
     6181 tctagattca ttcgaataat ccggtgattg gtgcgggctg gatagttcaa gcgacctcta
     6241 tatccttcgt tttccatgca aagcatgcag gtaggatagt ctacttttac cgcctcgcgt
     6301 tctgctgcga tttgttttgg atctttttct ggcttagata aattgattgt gatttccagt
     6361 tcaccatact ccgactctgc cggaaatttg atgttttttg cgatggctct tgttttgatg
     6421 tagtcatttt tcttacttaa attaaagaaa tactcagttg cttcctctgg gcttttcgaa
     6481 tagtgctgtg caaagaatgc attgacgact gaaggtggcg gtgtgagaaa atccattaat
     6541 tgcgcttcaa aaatctcttg ttctgctggc aattctgaaa gaacaccatt ttctcttgcc
     6601 ttagcgacaa gctgatccaa taagtctaca gattcttcct taactggtct tacttcaatg
     6661 ctttctagag catcttctcc taccatccct aaaagacgat tctgtaaata taagcggtcc
     6721 atttccatcc atccgccagc ttcgatcgca agtgtggcaa aatcacaaat cgtttgactg
     6781 attgacatat ctcattcccc tattcttcat aaccatttgg atggctgaca tgccaatccc
     6841 atgctgtttt gataatcgct ttgatatctg tgtattcagg tttccagccc agaatctctc
     6901 tcgctttttc acttgaagcg actaatcggc taggatctcc agctcttctt ggtgctattt
     6961 ctgctggaat ttcttttcct gtcacttctc tagctgcttc tagcatttcc ttgactgagt
     7021 atcctttgct gctgcctaaa ttgaagaagt tgctttcatt tccttctttc aagtattcca
     7081 aagctaaaat atgtgcagca atcaaatctt ctacttgaac ataatctctg atacatgttc
     7141 catctggtgt atcataatcg tctccataaa cagccaaggc ttttctttga ccaagagcta
     7201 cttgtaaaat gattgggaca agatgcgttt ctggtgtatg atcttctccg atagatgcat
     7261 ctgcttttgc tccggctaca ttgaagtagc gcaaagcgac atagcgcatg ccatatgctt
     7321 ggtcacacca cttcatcatt ttttccatca tcaatttact ttcaccatac ggatttttag
     7381 gattagctgg tgtatcttct gtgattggag actctttcgg ctctccataa gttgctgcag
     7441 tagaagagaa tacgatttta ttcacgttga actcgtgcat gacttcaagt aagatttgca
     7501 taccgtatac gttgttgttg aaatacatca atggtttttc gacagattct cctactaaag
     7561 agcttgcagc aaaatggatt actccttcaa tcggttcctt ttcaaatacg gaacgtagaa
     7621 attctttatc ccggatgtct ccttcataaa aatgagcatc tggatgtact gcttgtttgt
     7681 gacctgtgag caaattatct accacagcta cttgataccc cttttgaact agctggtcta
     7741 cagcgtgcga accgatataa ccggctccgc ctagtactaa aattgtcata aaaaacactc
     7801 ctttgattag taaaattgtg
//
LOCUS       DAZKMB010000119         7799 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000119 DAZKMB010000000
VERSION     DAZKMB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.122"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..641)
                     /locus_tag="ACG8V3_002402"
     CDS             complement(<1..641)
                     /locus_tag="ACG8V3_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0300881.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="ACG8V3_002403"
     CDS             complement(643..1407)
                     /locus_tag="ACG8V3_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0300882.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="ACG8V3_002404"
     CDS             complement(1415..2092)
                     /locus_tag="ACG8V3_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0300883.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="ACG8V3_002405"
     CDS             complement(2129..3514)
                     /locus_tag="ACG8V3_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0300884.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="ACG8V3_002406"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8V3_002406"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG8V3_002407"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG8V3_002407"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0300885.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="ACG8V3_002408"
     CDS             4453..5310
                     /locus_tag="ACG8V3_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300886.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="ACG8V3_002409"
     CDS             complement(5391..5726)
                     /locus_tag="ACG8V3_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300887.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="ACG8V3_002410"
     CDS             complement(5793..7163)
                     /locus_tag="ACG8V3_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0300888.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="ACG8V3_002411"
     CDS             complement(7241..7777)
                     /locus_tag="ACG8V3_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0300889.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2214 a         1661 c         1268 g         2656 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcat
//
LOCUS       DAZKMB010000120         7721 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000120 DAZKMB010000000
VERSION     DAZKMB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.123"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..2426
                     /locus_tag="ACG8V3_002412"
     CDS             99..2426
                     /locus_tag="ACG8V3_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0300890.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2423..3337
                     /locus_tag="ACG8V3_002413"
                     /pseudo
     CDS             2423..3337
                     /locus_tag="ACG8V3_002413"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3410..3925
                     /locus_tag="ACG8V3_002414"
     CDS             3410..3925
                     /locus_tag="ACG8V3_002414"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0300891.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3922..4368
                     /locus_tag="ACG8V3_002415"
     CDS             3922..4368
                     /locus_tag="ACG8V3_002415"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0300892.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4518..5333
                     /locus_tag="ACG8V3_002416"
     CDS             4518..5333
                     /locus_tag="ACG8V3_002416"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0300893.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5539..6711
                     /locus_tag="ACG8V3_002417"
     CDS             5539..6711
                     /locus_tag="ACG8V3_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0300894.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6873..7367)
                     /locus_tag="ACG8V3_002418"
     CDS             complement(6873..7367)
                     /locus_tag="ACG8V3_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300895.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7364..7558)
                     /locus_tag="ACG8V3_002419"
     CDS             complement(7364..7558)
                     /locus_tag="ACG8V3_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300896.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2610 a         1453 c         1531 g         2127 t
ORIGIN      
        1 aaaaagcccc aaactttcta tacatcaata aaataataaa aattaaattt agaataacgt
       61 gtaaaggatt atttgatgat tttttattga gaggagggat gaaaatgatt aagaaaactc
      121 aaaataaaat ccaacgtata tgtcaaaaac attttgtttc tagactcaaa gctgtatcta
      181 aaagaaaata cttacatccc attatgattt cgatcatctt gatttttact ttcttaggga
      241 gcttatcaag tatttatgta aacgtttata cagaaacgtc aaattttgct aaccaaacga
      301 cagaggagac aaatagtcca ccctattctg aaatacagtc catagaaagc accaatcagt
      361 ctagcaacaa cgagacaacc tcaaccacat cctcgactca actgattgat ccgacagaaa
      421 aagaaatcac agcagcaaaa gcagcagggg agaaagctta ttcagaaaca ggtagaagcc
      481 agacatttag tactgttgcg gccactgcga atttcaacgg acataccata ccagatggat
      541 atacagtgat atatgccaaa gatggagtca ttgttgcggt taacggatcg atgagtgcgg
      601 ataaaactgc gttacaaggc gtaaccaaag caatcgcttt tattgataac cgtccagata
      661 tccataatct gtttattggc tccacagtaa atggtactac tatcacattc ccaaaaagta
      721 tctatatggg ggtggctacc gctggaaata cgatcgattc agttacaccc aatgcggata
      781 aaagttattc ttttacaccc tcaacatggg gagggcttcc tgccggagga ggtatgagta
      841 ctttttcaat cggtatacag ttagcaaatg atattccatt cacggggtac tcgatcaaaa
      901 atgggcagat cgttgatggt aagggaaatg tttcaaattt tccaacctat caagcagcaa
      961 cggttcaggt caatgcgaat cggacaggaa ctttttctta tacagtcaat ggtggagcag
     1021 caaaaatcgg aacgtacggg actacattat ctggaatcca tgtgggtgac aaagtggtta
     1081 tcactccaac agcagtaacg ggctattctt ttgcacaaac aaacggttct ttcacagccc
     1141 tagaaagtaa cgtcgatacg gtgacctata caggcaaaca aacaccaatc actattaagt
     1201 atcaagacta ttttggacag atgattgctc cttctaaaat aatgaatctt acttacggaa
     1261 gtgctgcaca agacctgacg actaatacgc ctactatcaa cggatatacc tttacagctg
     1321 tttcagcgat agaaacaaag aaccaatcag caatgagtgt tttagctagt ttagatacaa
     1381 atggaaatgt aatcgtaaag gacgcaaatg gaaaacagat ttctgaagtc atcctatatt
     1441 ataagacaaa tgcaacagta tcaatcaatg caaatggcag taagtactat gatggtcttt
     1501 ctgttgtacc gatcgttaaa tatgccttta atgataaaac agaatcaaca tcccttttaa
     1561 acaatgatct cagtattgat caaatcacat ggaatccaac tgatttcaaa gcgatgaatg
     1621 aaaagggagt tgaagtaact gcgcctactg aaattggaac ctatacgtct tggcaattga
     1681 caaaaagcgg actagctaaa ctagctgcca gaacaaacta tttatttact atcgtgcaga
     1741 catctgatgt ctatacgatt aagcaaatca gcggtgcggt agagctcagt ggcagtaaga
     1801 catatgatgg aaaagcaggt gtcccgtcaa tccatgtcaa gctagcagag ggtgtcacta
     1861 gttcgcttac tccagtacaa gtggcactta gcagtatgga ttacacagca gatgcacaat
     1921 cagcaaaaaa tatggttaat gctggcagct atacaattaa actaacgaat agcggaatag
     1981 acaaagtcaa agcagcaaat cccaagttgt catttactga tatagccaac acatcaggaa
     2041 cttataccat ctataaagct aacgcagtga ttactgtcga tgacgctgcc tttaattatg
     2101 atgcccaaag tcactcgatt tcggttggaa atctccacgt taccggtgtt gtcttaggcg
     2161 aatcattgga ttatacccta acagttaaca gccggacaga tattggatca caaatagtaa
     2221 cgattagttt aggcgaagga agtgtaaaca ataattatac gattatcgca acagataccg
     2281 ctaagttgac gatcaatccg actctcttgc ttccttcgac aggtgggata ggtctactgc
     2341 cctttgtctt tttaggactg atctttattt tttctggctt cttttatttt atccatcgta
     2401 agaaaaaagc aggtgaacag cgatgaaaaa aacaaaaaat gtacgtgtgg atatatttgc
     2461 tgttctcttt tttatcctac tggtcttttt tataggttct ccgactgtgt cagctagaga
     2521 atcacctatt acatttactt tgcataatcg aatgttcgaa agcgataaga tgccaagtat
     2581 cagtcaaaac gacggaataa atatcccgga taactttcaa ggagtaaaag ggttgaacgg
     2641 cgttactttc gctatatatg atgtctcgga tgaattttac aagctgcggt cagaaggaag
     2701 tagtgtggaa gacgcacaaa gaaaactagc acaaaaaagt gatagtgaaa aaatactggc
     2761 agaaaatgtg acaaaaacag tagacgagga agagggcatt gcttctttta gtgcatcgga
     2821 caaagatgag caaggtcgtg acgctgttta ccgatttacg gaaataaaga cttcagatta
     2881 agtaaaagaa aaatcagcac catttgtcgt tgtgcttcct gtgacagatt catctaatca
     2941 taaattgacg accatccact tgtatccaaa gagcgaacaa aaaatacctg ctgcactcac
     3001 attaacaaaa acagtagaga ataagcagac agattttgca gatggggata aagtccctta
     3061 tctcatcacc gtaacaattc ctgagaacat caacgagata ggaacctata cgatcaaaga
     3121 tacagctgat cctcagttat ggctagaacc agaaacgatc gattttttga ttggtggaga
     3181 caagattcat acatttcaca cacaaaaaat aaaacatggt tttgtcttat cagattctgg
     3241 aaaagatctt agttcgtttg ctggaaaaaa attaacgatc cgttaccaaa tgacattgaa
     3301 agaaaacagt gaaaaaacga catttgataa cgatgtacgt ttgacgactg acaatcaaac
     3361 agtagaaaca catcttgcca tccaaacagg aggcaaacaa tttgtcaaag tggatcggca
     3421 aacgaaacaa aaactagcag atgctcaatt tctagtcaaa aaaaatgacc aatacttagc
     3481 gcaagaaaac gggataaatc attgggtagc caaagatgat tcaagagcaa cgatttttac
     3541 ttccagtaaa gatggaacat tttctgtcca tggattgtct tttggaagtt acgaactagt
     3601 ggagattaaa ccgcctaaag gatatatatt gagtcaatca accattctct ttgaagttga
     3661 aaaaggcagt tatgatccgc ttcatcctat accattggaa atcatcaacg aaccaaaagc
     3721 aaatccgacg aataatggaa aaacgccagt tcagatgcag acaaatggtc catcaaatga
     3781 aaaaaatgga ggaagctttc caaaaacaag cgaagagatg cttggaggtt tctcaatttt
     3841 agggttattg ctagtccttt caacagggac agcttggttt tataaaaaac aacaaaaagg
     3901 aaacaatagg agggaaataa aatgaaaaac atcaaaacaa aaatatggac gtttttagga
     3961 actgctatca tgctgcttcc tttcgttctc ggcctaggaa ctgcagaggt ttctgcagcc
     4021 gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt cacttcagca
     4081 ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga accgttgaac
     4141 ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa agacacagca
     4201 gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg attagctaac
     4261 ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt agaaacacca
     4321 aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctagac cctgacaaac
     4381 ctttagacac gattcaccta tatgctaaaa atgtcatcaa agaaagcaat atcaaaatcc
     4441 agaaagctgg gaacttttca ggggtagata agttagcagg ggcgcaattc cagatcaaaa
     4501 attcagagaa tcaattcgtg accggttttg atgaaaacac tggtgtcgct ctctatgatc
     4561 ctgctgagtc agcgttgaca ttcacaactg atactgaagg atccatcagt gtcaatggat
     4621 taatagacgg caattatact ttagttgaaa caaaagcacc agatggttat caattaacca
     4681 atccacaaaa agaaactact tttatagtaa aaaacggaaa attgcaacaa acaaccggta
     4741 tcatccatgg cgcagatgaa gccaatcttg ggaatccaaa cggtgtagtc aatcgggaaa
     4801 atacaactac ggataacacg attactgtag aaaatgtcca aaactacggt gatttccact
     4861 tcatcaaaca agatgtgaac acgcaagaaa aattagccga tgcagaattt tctattctaa
     4921 gagcaaaaga tccaactgtt aaattaatga aaaaaatagc aaaccttgcc tcaggagaat
     4981 acccatatgc ttggtcagat gaagtcacag gttctgactg ggaagaagtg aaattgattt
     5041 ctgattcaga agggaaattt tcaatcgaag ggctgaaaca aggggcatac tgccttcaag
     5101 aaacaaaagc accagaaggc tacgcattac ctaagaatga agctgcgtac actgaattta
     5161 cagtagatga tgatccaaca acttctgatg aaacaacgat cgataaccaa cctaaaggaa
     5221 tcttgccttc aactggtggt atggggatca tcgtattcat cttggtaggt actgctttag
     5281 ttggaggagc agtgatctat ttcaaaaaac gccatcaaga aacagaagct taaactattg
     5341 gaaacaaaaa tcatacgata aataaacgat agtgattcaa atggggagaa aggttgtgac
     5401 aagtggtcgg tctcttggta ttcgctcaac aaacggcaaa ataggaggaa ctattttgcc
     5461 gtttgcttcg ctaatactca ggtcaaggcg ctgatttcac aacctcttca tatacatatt
     5521 agaaaatagg tggacatgat gaataaaagc agaataagaa tactggacat cttcatggca
     5581 atcattttag tcgttgggat cgggatcttt tcttatcctt ttgtagaaga ttcactgaat
     5641 gattttcttg cccagcaaat gatcatccat tatcaaaagc aagcatcgaa taagaattca
     5701 gaggaaataa aaaaacagca ggaaaaaatg acaaagaaaa accaacagtt ggcagagcaa
     5761 aacgtatcac cagggatagg cagtttcaac caagcagttg atgcaaaagc attaaaagat
     5821 ttacctagta atgccttttt tatggaacat atgcttggag tcatcgaaat cccaaaaatc
     5881 aatgtcagct tacctatatt cgatcagaca acggaaatat ttttgcaaaa aggaacgtca
     5941 ttattggaag aaagttctta tccgactggc gggaaaaaca cccatgccgt tctttctggg
     6001 catcgtggac tcccagaagc aaaattattt acagatttgc ctaagttgaa aaaaggagac
     6061 cagtttttca tccagatcaa tggaaagaca ttggcgtatc aagtagaaaa aatccaagtg
     6121 gtcttacctg atgaagtaga ttctttgggt atccaaaaag gcagagatct cgtgactttg
     6181 cttacctgta ctccttatat ggtcaataca catcgactgc ttgtgacggg gcatcgtatt
     6241 ccttatcaag caaaagaggc aaaaaaagcg atccaaggaa ttgatcaatg gaaaaaatgg
     6301 aaatttttta tttggtttat cggtatacta cttggatcaa tcggcttagt ttggctactc
     6361 atcgcttatt tagattcttt ggccatcgct aagcgaaact atcctttatc tttttatgta
     6421 aagaacacaa acggacgacc aatcgaagga atggtctttt ctgtaaaaac gctcaacggc
     6481 aaacactata ttactagaga aaaagttcca tttgtgaaag cttctgatga atacggacta
     6541 gtgagatttt ctgatttaaa aggcaggaac tatcgcttgc aacatgagga gctcctgctt
     6601 aaaatccatg taaaacataa acattcgaaa cagttttcaa tgaaattgaa aaaaggaaga
     6661 tataaactgc gaaaagaaaa agaggtgtac tatttgattg agaaagaatg aatgaagatg
     6721 tccaaaaaag cagagggaac agactaaatg aaaaaagaga gaagctgacg aaatggacgt
     6781 gtaaactgtt atttttggtt tactatctgg aacgccatct tctatttttt tatgtttcag
     6841 aagaatttta ttattatagt ataacttttg aattagtttc cataacattt ggagcaaggg
     6901 gttaaaccac gagcttgagc ttcgcttaaa gtggcagggg tatacgtacc atttccacat
     6961 ttatgtgtat ggtattttga accggtcggt gtgacataga cagtttgttc attattttgc
     7021 tcaggagctg cggcttgtgc ctgctgagag gcctgttcag ctgctgattg ctgtcgcgcc
     7081 tgttctgctt gttgctgtgc gattgcttct tgcctcgcct gttctgcttg ttgctgtgcg
     7141 attgcttctt gttgcgcttg ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt
     7201 tcttcttctg cttcttttgc tttgattgtg gaatcaattg cagcaagacg gcttgtcaac
     7261 tcttgtttgt tttcaggtag cgcttggata gctgctgaag cagttgcata gttttctttt
     7321 gtaggatttg cttcagcttg tgacaaagca gattccgctt ctgtcacttt ttttctagtg
     7381 cagctttttc cgctttttgt ttctcttctt ttttcttttc aatttgttct tgttctttcg
     7441 atgaatctgc cgtactttcg gagatcgtgg tactgctgct ggaagcctga tctgaacttg
     7501 tgctttgagt agtagacgta gtagcaccac acccactcaa caaacttcct gcgaccaaaa
     7561 acaatgcaac taattttttc gctaaatttc ctccttaagt aaaaaacaac cgttttcaat
     7621 aaatgtcctt ttcaattgtt tacttacttg ttataataat tgtgttatga atagtgattg
     7681 aggaaactta atctaatccc gtgttgatac acgggatttt t
//
LOCUS       DAZKMB010000121         7601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000121 DAZKMB010000000
VERSION     DAZKMB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.124"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..525
                     /locus_tag="ACG8V3_002420"
     CDS             16..525
                     /locus_tag="ACG8V3_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0300897.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            618..1780
                     /locus_tag="ACG8V3_002421"
     CDS             join(618..894,894..1780)
                     /locus_tag="ACG8V3_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0300898.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2060..3040
                     /locus_tag="ACG8V3_002422"
     CDS             2060..3040
                     /locus_tag="ACG8V3_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0300899.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3162..4580
                     /locus_tag="ACG8V3_002423"
     CDS             3162..4580
                     /locus_tag="ACG8V3_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0300900.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4651..6120
                     /locus_tag="ACG8V3_002424"
     CDS             4651..6120
                     /locus_tag="ACG8V3_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0300901.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6130..6597
                     /locus_tag="ACG8V3_002425"
     CDS             6130..6597
                     /locus_tag="ACG8V3_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0300902.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6633..7505
                     /gene="scrK"
                     /locus_tag="ACG8V3_002426"
     CDS             6633..7505
                     /gene="scrK"
                     /locus_tag="ACG8V3_002426"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0300903.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
BASE COUNT         2606 a         1154 c         1569 g         2272 t
ORIGIN      
        1 tagggctata ctatattggt acaaggaata gaaagaggtg tttatgtggt tgataaaatt
       61 cagtttaatc aagttataga agaattcatc aaagtaaata atttaatgac ccatattcaa
      121 aaacgaccaa tcagaataac taatgatcta aaactcagta cgagtatgat tcatttgatt
      181 gatgtgattg gaaattatcc tgaaactagt attacggaat tagctgatcg attaggcgtt
      241 accaaaggag ctgtatccca acagattccc actttagaga aaatgaatct tataagcatt
      301 tctcaaaaaa aaggaaataa gaaaactaaa cttgtatcgt taacaaaata tggtcgaaag
      361 gtgtacgaat cacataatta tttacatgaa gagctttata gctcaataaa tgaagcgtta
      421 gaatcattct caccagacca aatttcaacc attaaacaga ttctagaaaa agtatcttat
      481 acaatcaatg aatatcaagt aagtctgaca agaaaggaag attgacatga aaaaaatgat
      541 ttttgaatcg gctacactag actagacaga aaaaataagg tgtgtaaaat aaatgaaaac
      601 acactggagg aatttttatg tcaagacgtc aacgtagaac ctattcaaaa gaattcaaac
      661 aacaagtcgt cgatctttat ctcgcaggaa agccccgagc tgaaatcatt cgagattatg
      721 agctgactac ctcggccttt gataagtggg tcaagcaagc gcaaactact ggctcgtttc
      781 aggaaaaaga taacttgacc cctgaacaag tagaactaat tgcattacga aagaaaaata
      841 aacagcttga aatggagaat gacattttaa agcaagcggc gctgatattg ggacgaaaag
      901 acaagtaatt gataccaata agcacaaata ctcaatatca gcgatgtgta aagtgttaaa
      961 catctcttgc caaacctatt attatcaagc caaaccagtt gaagatgaat cagaactgga
     1021 agaactcgtt caagaagaat ttattcgcaa tcgaaaagct tatggtacca ggaagctgaa
     1081 gcagtgcttg gccaatctag gtcttcaagt gagtcaacgc agaatcggcc gtatgatgaa
     1141 acagcgtggc ttgcgttcaa catacacatt ggctcatttc aaattccatt cttctacttg
     1201 taacgaagcc aagaccgcca atattctaga cggaaccttc ctacaggaaa agtcattaga
     1261 agcgattgta accgacctta cctatgttcg tgttgggaag aaatggcagt atatctgcct
     1321 gattcttgat ttgtttaatc gagaaatcat cggctattca tgcggggaga agaaagatgc
     1381 gacgttagtc aaggaagctt ttgcaaagat tccgtactcg ttgaataatg tacaactttt
     1441 tcacacggat cgtggaaaag aattcgataa ccagacgatt gacgaaattt tatgggcctt
     1501 taatatcacg cgttccttaa gcaaaaaagg ctgcccctat gataacgccg ttgcagaatc
     1561 cacctataaa tctgtcaaag tagaattcgt tcatcaatac cgctttgaga cattggctaa
     1621 tttgcgatta gagctatttg attacgtcca ttggtggaat accatccggc tgcacggcac
     1681 gttaggctat gagacaccga tcagttttcg acagcagaga ttggcgcagc gaatccttga
     1741 taatgagctc ggatgtgata ccggtggcga ggcaccttaa ttatttgaat gagcttctgc
     1801 cttagagaat cagattcgag gaggctcgtt gtcaagggca gtcggaacac cagcagcaag
     1861 cttacgcaat accttataat ttttgtcaaa aaaagtgttg ccattccaca tatatcttta
     1921 aacagctatt gccagataaa aataaatgat attatatata taaatactta tccgaaaagg
     1981 ggaatgagag agtcaaggtt cttggaatgt cgaatagtcg agtaggtaga ttttctatct
     2041 acaaccttgt tgtttaaaaa tgaaaaaaat tggaattaga gaagttgcga aagaggcaaa
     2101 tgtttctcct acgactgtgt cgagggtatt gaatgatcgt ggatatttga gtgaggaaac
     2161 aaaaagaaaa gtttttgatg ccatgaaaaa attagaatac tatcctaatg aactggcgag
     2221 agcacttttt aaaaacgaaa catataccgt aggtttgatt tttcctacaa ttactaatcc
     2281 atttcacgct gagttaattc aagatatcga gttcgagctt tccagcaggg gatataaagt
     2341 gttgctgtgc aatagcttaa acaatccaac aaaagaaaaa gagtacttga cgatgttgag
     2401 aaagaatcag gttgatggaa ttatagtagg aacgcacaat aaagatattg cggattatga
     2461 tattccaggg ctacctattg tagcgattga tagaagtctt ggtgaaaata cagttacagt
     2521 atcttgtgat aattacaggg gtggggaatt agctactcaa ctacttgtaa atcatggctg
     2581 tcagaatata ctgtgtattc ggggagatag taagataaag ctacccgcga atagtaggac
     2641 tttagcatat aaagatgttt tgaataaatt catgctggaa ccgttagtcc tagaagtacc
     2701 atttgtaaaa gaaatcaatg aaaaaaatga aataattaag acatatctgg aaactcatcc
     2761 aaatattgac ggggtttttg caggagatga tttattggct tctttggcga ttaacaactt
     2821 aggaagttcg ggcaaaagag ttccaaaaga ggttaaagtg attggatttg atggagctca
     2881 acaaacattg atttataatc cagaattatc aactattcaa cagcctatag atcaaatatc
     2941 taaagtagca gtaaaaaaat tattgaatat gattaagggc gaaaaggaga cggatgaact
     3001 ttcacttcca gtaaagctca taaaaaaaaa tacagcataa gttaaatcgg cgaaaaattc
     3061 cttcgtcgat ttaaaaatat ttttatgaaa cgggttgaca tatgaaacgg gttgacatat
     3121 attagttgtg aagataaaaa ccttttcact ggaggaagaa aatggagaaa ttattaatta
     3181 aaagcaataa tcatcaactt tactaccaac ctaaggatgt ttgggtcgga gatgttatgc
     3241 cttacgggaa agaaggaaaa ttctttattt atcatcaacg tgatactaga aatccagttc
     3301 cttttggaga accgtttggc tgggctttag caactacgaa agattttgtt cattttgaag
     3361 actttgggga atctctttta agaggggatg acaatgagag agaccagttt atctacgctg
     3421 gttctgtatt tgaggcaaat ggaaaggttc acgcatttta tacagggtat aatcgtgaat
     3481 tcttagcgca gggaaagaca tctcaagtgt tgcttcatgc tgagagtgat gattttgttc
     3541 attgggaaaa atcagaaaca gcattggagt tagaaccaca agaagggtat gatattcgaa
     3601 actggagaga tccatttgtc gtttgggatg atgaaaaaga agaatatttg ctaattttag
     3661 gggcgcgaaa gggtcaggat aaacatctac aaaccggacg tttagtggaa tttacatcaa
     3721 aagatttaac taattgggaa ttcaaaggtg atttttgggc tccgggattg tataccatgt
     3781 ttgaaatgcc agaacttttc aagattggtg actggtggta tcttgtctat tcagaatact
     3841 cagatggaaa taaaatactc tatcgaatga gtcaaagttt ggaaggtcct tggattaagc
     3901 ctaaagatga agcttttgat ggacgggctt attatgcagg tagaacagct tatgatggaa
     3961 aacgtcgagt cctatttgga tgggtaccaa caaaagatca agaaaatgat aaaaacaatt
     4021 atttatgggg aggtactttg gttcctcatg aagtctatca aaaagaggat ttttcacttg
     4081 gggtaaaacc catagacgaa gtgtggaatg cattcataaa tttacatgat attgccgatg
     4141 tagagataga aacagcagac acaagagaag aagtaacgct tgtcaaaaat agtggaactt
     4201 tgtttaattt agaaacaaca atttcatttg atgagaatct tagtgacttt tcactgagat
     4261 tatataaaaa ccttgagaca gatgaatctt acgaatatcg cttcaattta gatgaggggc
     4321 gtcttacatt tgataaaaac ccttgttacc cttggtttca atatatggat aaaggactta
     4381 ttagacctat atctttaaag cctaaccaat cttataaact aaaagtgatt gttgatgaca
     4441 atataatgac actttatatt gatggaatcg ctcttaacgc tcgtgtacat ggttgttttg
     4501 gtcaagcaat tactattaca gcaacaaatg gatcaatgac tgcaataaat accaagtttt
     4561 caaatgaaat aaagaaataa taaacgtcaa tcatgtggca ggtctgaaaa tcaaacatga
     4621 cctgtcacat ttaaaaggga ggataaatga atggactaca aaaaaattgc cgaagatgta
     4681 ttggaaaacg ttggaggaaa agagaatatc tcaagcgcta cacactgcat tactcgttta
     4741 cgtttaattc ttaaagacaa aagcaaagcg aatatggaaa tgcttgagga aatagaaggt
     4801 gcaaaaggtg ttcttttcaa tagtggacag ttacaagtaa ttttcggaac aggtataatt
     4861 gaaaaggttt acaaagagtt cgttgaattg acaggagcta aggaagtatc cgtttctgaa
     4921 atgaaagacg aaggagtcaa taattcagga aatagacttc aacgagcttt taaagttttc
     4981 tcggatatct ttattccgat cattccagct tttattgggg cagcaatggt tttaggaatt
     5041 cgttcgttga ttaccactcc tggaatgttt ggaatggatg gctcattcgc tgatcacaat
     5101 gtattcctaa atgatttatc caaatttctg ggtattattg caaccacatt tgattattta
     5161 ccagttttag ttatgtattc tgcaacaaaa cgttttggcg gaaaccctat tttaggatta
     5221 cttgtaggat ttgttatgat tcatccagga ctagccaatc ggaatgattt cgtcatggga
     5281 actataaagc ctgattattg gcatttattt ggattaagca ttccagcagt tgcgtttcaa
     5341 gggggtgttt ttccagcaat tttgacttct tggtttttag caaaagttga gaaaattaca
     5401 aataaatatt ctcctcaagt acttgcttac attttagtcc caactgtgac aattgttctt
     5461 gccaactttg cactattttt agtgtttggt ccattgggga attttatcgg taatgtttta
     5521 ggtggaatca ttgatttcct atatatgaag atgggtgcct ttggtgcttt tgtttttgca
     5581 ggcgcgttac aaccattagt tgtaacagga acacaacatg ctattcaagg tattgaggca
     5641 aacttagtcg caacaactgg attcaattat atccaaccta tttggtcagt ttcaattatt
     5701 gcccaaggtg gcggttgtat tggtatgtat ttactcttta agaaaaaatc taaagataga
     5761 gatattgcga tttcaagctt tattccaaca ctggtaggta ttactgaacc ggctatcttt
     5821 gctgttaatt tgaaatattc aatcgttcct tttgtttgct cgtttattgg tgctggtttt
     5881 ggtggagcat ttatgaaaat ttttgatgta aaagccattg gacaaggatt gactgtattg
     5941 ccaggtctga ctattgctaa tccagttctt ccttatatat ttggtaatct catagcattc
     6001 attttaccaa tcatttttat tctcgcttat gataaatata aagtgtttat ccctgaagct
     6061 aaacaccgta aatttgcaga aaaaactgac tcaaaaaaat tgccagttca agtaaagtga
     6121 gatgagagca tgttaaattt ttttagaaaa aatgaaataa gcgttgtagc acctgcaacc
     6181 ggtgtttttg aaaaactgga gaaattaagt gatccagtct tttcaaaagg aatgatggga
     6241 caaggatttg caattgatgc aaaaaacgaa acgattgtat ctccaatcga tggttttgtt
     6301 tcaagtattt ttcctacaaa acatgcctta ggaataaaaa caaaatcagg aattgagata
     6361 cttgttcatg taggaataga tacagttgag ctaaatggtg agggatttga tattaaagtt
     6421 caagaaggac aaaaagttaa ggctggggat attcttatga ccattaattt tgtagttttt
     6481 gaaaagaatc acaagactaa agatgtcatt gttgtttttc cagactacca agacgaaatt
     6541 ttcattccga aacttgaaga agtaaaacaa ggacaagaat tgtttgttct tggctaatga
     6601 taagtaatta ataatagaat cgaggaaaat gtatggtttt tggggctatt gaagctggtg
     6661 gaacaaaatt tgtttgtgcc attggagata aagatttgaa catatttgaa aaagtcagct
     6721 ttcccacgac tacacccaaa gaaacaatga atttagtttt agattttttt aatcatcata
     6781 aagatgattt agaaggtatc ggagtgggat cgttcggtcc tattgatatt cattgtgaat
     6841 ctaaaacgta tggcttcatt acatcaacgc caaaacttgc ttggcaggat tttgatttta
     6901 ttggaagttt aaaaaagcaa cttaatattc caactgcttg gacgactgat gtaaatgcct
     6961 cttgctatgg tgaatatgtt tctggaaatg gcagaggcct ttcaagtgtt gtttactata
     7021 ccgtaggaac gggcatagga ggtggcgcta tccaaaacgg aaacttcatt gaaggtttta
     7081 gtcatccaga aatgggacat atgcttgtaa aacgacacaa taaagacaac ttttctggga
     7141 catgtccatt ccacggagac tgtttagagg gcttagctgc tggtccggca atcgaaagaa
     7201 gatcagggaa aaagggacaa gaagtgactg aaaatgacgt tacttgggaa atagaagctt
     7261 attacattgc tcagtgtgca tataatacaa cattgatgtt atcacctgat gtcattatat
     7321 ttggtggcgg agtaatgaaa caaaaacact taaaaaagaa agtacaagat aagtttattg
     7381 agttgatcaa cggatatgtc gctattcccg atgttgacaa ttatattatg acaccaaaat
     7441 tgttagataa ttctggtatt gtaggttgtt tagctttagc aagagacgtt aaagaaaaat
     7501 cttaatttgg aaaaaagatc gtctcgacta tttgtttggt tgatgcgatc ttttttttca
     7561 attagtaatt gaagggacgc ctaaaagtga aaaaaaaaat g
//
LOCUS       DAZKMB010000122         7600 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000122 DAZKMB010000000
VERSION     DAZKMB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.125"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..427)
                     /locus_tag="ACG8V3_002427"
     CDS             complement(137..427)
                     /locus_tag="ACG8V3_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300904.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="ACG8V3_002428"
     CDS             complement(545..805)
                     /locus_tag="ACG8V3_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300905.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="ACG8V3_002429"
     CDS             complement(942..1355)
                     /locus_tag="ACG8V3_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0300906.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="ACG8V3_002430"
     CDS             complement(1811..2296)
                     /locus_tag="ACG8V3_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300907.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="ACG8V3_002431"
     CDS             complement(2435..2650)
                     /locus_tag="ACG8V3_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0300908.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="ACG8V3_002432"
     CDS             2874..3674
                     /locus_tag="ACG8V3_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0300909.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="ACG8V3_002433"
     CDS             3693..5561
                     /locus_tag="ACG8V3_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0300910.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="ACG8V3_002434"
     CDS             5554..6045
                     /locus_tag="ACG8V3_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0300911.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /locus_tag="ACG8V3_002435"
     CDS             6033..6587
                     /locus_tag="ACG8V3_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0300912.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..7600
                     /locus_tag="ACG8V3_002436"
     CDS             6605..7600
                     /locus_tag="ACG8V3_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0300913.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctga
//
LOCUS       DAZKMB010000123         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000123 DAZKMB010000000
VERSION     DAZKMB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.126"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACG8V3_002437"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACG8V3_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0300914.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACG8V3_002438"
     CDS             1483..1935
                     /locus_tag="ACG8V3_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300915.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACG8V3_002439"
     CDS             1949..2638
                     /locus_tag="ACG8V3_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300916.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACG8V3_002440"
     CDS             2763..5114
                     /locus_tag="ACG8V3_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0300917.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACG8V3_002441"
     CDS             5194..5589
                     /locus_tag="ACG8V3_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300918.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACG8V3_002442"
     CDS             5914..6531
                     /locus_tag="ACG8V3_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0300919.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACG8V3_002443"
     CDS             6572..6871
                     /locus_tag="ACG8V3_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0300920.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACG8V3_002444"
     CDS             6905..7072
                     /locus_tag="ACG8V3_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0300921.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACG8V3_002445"
     CDS             complement(7117..>7537)
                     /locus_tag="ACG8V3_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0300922.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAZKMB010000124         7317 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000124 DAZKMB010000000
VERSION     DAZKMB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.127"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(406..780)
                     /locus_tag="ACG8V3_002446"
     CDS             complement(406..780)
                     /locus_tag="ACG8V3_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0300923.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="ACG8V3_002447"
     CDS             complement(803..2203)
                     /locus_tag="ACG8V3_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFQ0300924.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="ACG8V3_002448"
     CDS             complement(2207..2617)
                     /locus_tag="ACG8V3_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0300925.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="ACG8V3_002449"
     CDS             complement(2635..3126)
                     /locus_tag="ACG8V3_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0300926.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="ACG8V3_002450"
     CDS             complement(3136..3927)
                     /locus_tag="ACG8V3_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0300927.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="ACG8V3_002451"
     CDS             complement(3920..4693)
                     /locus_tag="ACG8V3_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0300928.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>7317)
                     /locus_tag="ACG8V3_002452"
     CDS             complement(4849..>7317)
                     /locus_tag="ACG8V3_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0300929.1"
                     /translation="SAQDIAMKFCIKRNVASHYLNQLFSDGKLLKNDSVRPVMFKYNQ
                     QKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLPLIIKGNSGVGKSFLASLIYQYA
                     LDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKKGAFTGAEKDTAGMLSSANGGYL
                     FLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEYANVRLILATTEDKNTSLLATFR
                     RRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITIDSKIFCSIELFTDLLSKTFEGNV
                     GELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWIDPEISIDLKKMFLKSLSEIDLI
                     ELFQNHISIEESMSQLRDRILKECAGSSEYNYLESDEFIALRNYVVNKISPIIDSTGL
                     CLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFRMKNDKQKLLANEIWSSLEVEES
                     KQELLLKWILFLVKKFYVKIPETHCLIVMHGKITASAIARETNKLLNTYVYEAFDMPI
                     EGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTAL
                     AIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDI
                     LKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTTEFYIQGIDCLNLENLIN
                     GNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEK
                     YYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIK
                     DIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         2359 a         1434 c          950 g         2574 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaggtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt
     5101 ttcatacaaa tatagtactt tttctatctc attgaatact tttttggaat ctaaaaagcg
     5161 aagcttagta ctagcgcctt caatagtaaa gagtttaact aattcattta agcagaactc
     5221 acagctatcc gagtccatat acttatttaa tttttctaag gtttgattcc cattaattag
     5281 attttctaaa tttaaacaat cgattccttg aatgtaaaat tcagtggttg aaagaacaca
     5341 aattgtattc ttaaaaatca tagtatcttt ttcctttgct attttcttta atgcgttaaa
     5401 atcaaaaatc aaaatctcca cttgattatc gagataccgt tttaaaatat ccttaacttt
     5461 ttcagaaatc ccctcaccag agagacaaga tactattaca tttttctttt gtgataatcc
     5521 tttataatac tgaactttaa cttgaaacga atcaaaatcc atctggtaaa aatgactaat
     5581 tggctttttt tggcaaactt gaatcccaca ttcaatcgct aaagctgtag aaacattgtt
     5641 taaaattacc aaatcaccta ttacattgga ttcaattttt tcatacattt gttccaatga
     5701 tcccatatca actagcagaa tgagtccatt cgtcgtatct atacttttac aaaaattgtt
     5761 gattaggtta attaagtcac tagtctcacc ttcaattggc atatcgaaag cctcataaac
     5821 atatgtattc agcaacttat ttgtttccct tgcgatcgca gaagctgtta ttttaccgtg
     5881 cataactatc agacaatggg tttctggtat tttcacatag aacttcttta caagaaaaag
     5941 tatccatttc aacaaaagtt cttgtttact ttcttcgact tctaaactac tccaaatctc
     6001 atttgccaat aatttttgtt tatcattttt catacgaaat tttctgaact tagacagcaa
     6061 aaaatttctt gatgtttctt caattacatc aataaacatt ataaatagac tgatatccgc
     6121 caagaattca tctaataaac atagacctgt cgaatcaata attggtgata ttttattaac
     6181 aacatagttt cttaatgcta taaattcgtc actttctaaa taattatact cagaagaacc
     6241 tgcacactct ttaagtattc tatctcttaa ttgactcatg ctttcttcaa ttgaaatgtg
     6301 attttgaaaa agttcaatca aatcaatttc agaaagtgat ttaagaaaca ttttttttaa
     6361 atctatacta atttctggat ctatccaaaa accgctgtct agtttgtccc ctaaataaat
     6421 tgaagacttt ctcttatttc ctaaataacc ttctgcacaa agaagcttga tttcattcct
     6481 caattctcca acatttcctt caaatgtttt cgataaaaga tcagtaaaaa gttcaatcga
     6541 acaaaatatt tttgaatcaa ttgtaattgc ttcatcttta agaaattcaa ataataattg
     6601 aattttttca gatgtacttc tattattgta atctggcaga gtgatttcag ctgggatgcg
     6661 tcttctaaaa gttgctaaca aagaggtatt tttatcttct gttgtggcta agataaggcg
     6721 aacatttgca tactcccatt gcacactttc accaagttta cgatactttt gagaatccat
     6781 aagaagaaac aatttctctt ggttttctgc acttagattg tgaacctcat ctaaaaaaag
     6841 gtatcctcca ttagcagaag atagcatccc tgcagtatct ttttcagccc cagtaaaggc
     6901 tcctttttta tatccaaata aaactgctga caatagctct ggattatttg cataatctgc
     6961 acaattcaca acaacaaatt ttgcgtcatt atgaattact tttctatcaa gagcatactg
     7021 atagatcaaa cttgctaaaa aactttttcc aacgccactg tttcctttta ttattaatgg
     7081 gaggccatta gggggataca ttacagtagc tttgcattta tctatagtgg atttcaaaga
     7141 tatgtctgct ccaataaact tagaaaagca gtcttttggc ttttgttgat tatacttgaa
     7201 cattactggt ctaacagaat cattctttag taattttcca tctgaaaata actgattcaa
     7261 atagtgggaa gcgacatttc ttttaataca aaatttcatt gcaatatctt gagcaga
//
LOCUS       DAZKMB010000125         7218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000125 DAZKMB010000000
VERSION     DAZKMB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.128"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(230..907)
                     /gene="phoU"
                     /locus_tag="ACG8V3_002453"
     CDS             complement(230..907)
                     /gene="phoU"
                     /locus_tag="ACG8V3_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0300930.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(920..1678)
                     /gene="pstB"
                     /locus_tag="ACG8V3_002454"
     CDS             complement(920..1678)
                     /gene="pstB"
                     /locus_tag="ACG8V3_002454"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0300931.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1694..2500)
                     /gene="pstB"
                     /locus_tag="ACG8V3_002455"
     CDS             complement(1694..2500)
                     /gene="pstB"
                     /locus_tag="ACG8V3_002455"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0300932.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2515..3399)
                     /gene="pstA"
                     /locus_tag="ACG8V3_002456"
     CDS             complement(2515..3399)
                     /gene="pstA"
                     /locus_tag="ACG8V3_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0300933.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3399..4319)
                     /gene="pstC"
                     /locus_tag="ACG8V3_002457"
     CDS             complement(3399..4319)
                     /gene="pstC"
                     /locus_tag="ACG8V3_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0300934.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4434..5291)
                     /locus_tag="ACG8V3_002458"
     CDS             complement(4434..5291)
                     /locus_tag="ACG8V3_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0300935.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5628..7076)
                     /gene="cls"
                     /locus_tag="ACG8V3_002459"
     CDS             complement(5628..7076)
                     /gene="cls"
                     /locus_tag="ACG8V3_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0300936.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2281 t
ORIGIN      
        1 tttgcattct ttatcctttc tatgaaatga tgaaaagggt ttgtgacatg ttttgtcaca
       61 aaccctttcc cgaataaacg gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa
      121 acgaaaaata tgaggagcta ttttttgttt gactgttctt attacttgat gtagaacagc
      181 tttttcacaa cctcttttca taaacaaacg gcataatcac agcttcagtt tagaaaatct
      241 cattgtggtt cgtatttaac tcagtgattt tccctgtagc caagtagacg atccattcac
      301 agatgtttgt cacatagtct ccgatccgtt ctagataact ggcaacattc aagtagtctg
      361 ttccactgat gactgtctca ggattttcct gcatattttt gattgttgca ttatagattc
      421 gattgaaata gtcattgact ctttgatcca tattggcgat cgtacgtgcg tctttttcgt
      481 ctgttttgac ataagcaact aggacgttgt ccaccatttt tttcacataa tcggacatat
      541 cggagatctc tttttcgatt tccggtattc tcgtctgccc ttttacgcga atcgtggatt
      601 tagcaacaga caccgcatga tcggccatac gttctaaatc cgagctagct ttcatgaccg
      661 taatgatcat acgcaaatca gttgtaactg gctgttgcaa tgcgatcatt tcaaaacttt
      721 tcttctccaa gcgtgtttcc atgttattga tatttatatc gttttcgata acttcttggg
      781 ctaagatttt atcgtgtttg atgtatgcac gaactgactt gtgcacggcg ctgctcacca
      841 tcatccccat ttcgtagaac tgattatgga gattcagcaa ttcttcttca aattgtgtac
      901 gtagcattcc gtttcctcct tatccaaatc gacctgaaat ataatcttct gtctcttgtt
      961 tttctggatt catgaatatc ttcttcgtat cgttgtattc gatcaagtga ccatctaaaa
     1021 agaatgccgt cttatctgaa atacgtgaag cttgagacat attatgggtc acgatgatca
     1081 tcgtataccg ctctcttaat tctaacagca tactttcgat tttcccagta gaaacaggat
     1141 caagcgcact tgttggttca tccaataaga tgatttccgg attaactgcc aaaacacgtg
     1201 cgatacatac acgctgctgc tgcccgccgg ataaagataa agcactttta tgcaatttgt
     1261 ctttcacatc atcccaaaca gacgctgcct tcaggctttc ttcaactact tgatcaagga
     1321 cttttttatc tttttcccct tttaatttca atccatagat gacattttca tagatagaaa
     1381 aaggaaatgg gtttggctgc tggaatacca tgccgatttc ttttcttagt tcaacaacat
     1441 ctgtttttgg accatagatg tctttgtctt tatataaaac acttccggtg atcgttacat
     1501 tcggaatcag gtcattcatc cggttcaaag aacgcagata agtggatttt ccgcaaccag
     1561 aaggaccgat catcgcagtg atttcccctt gattgaaact tagatcgatc ccttttagtg
     1621 cttctttctt tccataatac agatgaagat ctttagatga aataatttct tttgtcatgt
     1681 tgaggcctcc gtcttaacca aaatgaccgg acacataatc ttcggttgcc tgaatttttg
     1741 gtctggtaaa tatcttgtta gttttgtcat attccattac ttttcctaga tagaaaaagg
     1801 cagtatagtc gctgatacga gctgcttgtt gcatgttatg cgtcacgatg atgatcgtat
     1861 agtcttcttt caagtttaca agtgtttctt caaccgttcc tgtcgaaata gggtccaaag
     1921 cacttgccgg ctcgtctagc agcaagatat caggtttcat cgcgatcgca cgagcaatgc
     1981 ataaacgttg ctgttgcccg ccagataaag ccaaggcact tttgtccaag ctgtctttta
     2041 cttggtccca caaggcagct tgtttcaagc ttgtttcaac gatttcatct aatttctttt
     2101 tgtttttttc gccgtgctgt ttcaaagcaa atgtaatatt gtcataaatc gacttgctga
     2161 acggattagg acgttgaaaa accatgccaa tccgtttgcg catctcataa acatccactt
     2221 gaggcgcatt aatatctacg ccttgataca tgattttccc agtgattttc gtattagcga
     2281 tcccatcatt cattcgattc aacgaacgaa gatacgtaga ttttccgcag ccggaaggac
     2341 caatcagtgc agtgatctta tttttttcaa attccagatc cactcctttg attgcttcat
     2401 tgtcgccata ccagacatgt atatcatccg tatatagtgc tacttctttc tcttttggca
     2461 tggtgatgac atttgtttca ttccagttat attttttcat tttgcttctt ctccttaggc
     2521 tgaagtcatt tttcggtata atcgtttgcc aatcgcacga gcgccgaagt tgaacagtaa
     2581 aacagcaatg atcaacacgg cagaagcgcc agccgaaaca gaaacaccat caggcatcgt
     2641 gccttctgta ttgatcttcc aaatatggac agctaatgtt tcagcttgac ggaaaatgct
     2701 gatcgggctt gaaacactta atggattcca gttcgtaaaa tctaacgcag gtgcactttg
     2761 tccagccgta tagatcaatg cagctgcttc accaaaaatt cttccagaac ttaggatcac
     2821 accagtcaaa atccctggtg tcgcttctgg aaccaccact ttcatcactg tttcccagcg
     2881 tgacaaacct aaggccaatc ctgcttctcg ttgtgtgtaa tggaccgctt gtaaagactc
     2941 ctcaactgtt ctagtcaaaa gaggcaagtt gaatacagtc aaagccaatg caccagaaat
     3001 aatcgagaaa ccatactgga attgaataac gaaaaccaag aaaccgaata aaccgacaac
     3061 gacagaaggc aacgagctca agatttcaat cgacgtgcga atcacatctg ttagccagtt
     3121 ttttccagca tattctgaaa gatagatacc cgcacctaaa gaaataggaa aactgatgat
     3181 catcgtgatc aaaagcaaat aaatcgagtt aaatagctgg atcccgattc caccaccttc
     3241 ttggtaggct ttggaaggtt cggtcaagaa ggaccatgaa atgtgcggta ttccccgaac
     3301 taagatataa agtagtaaag ctgctaaaat caatacgatg atccccgaaa ctatatataa
     3361 aacacctgta gcaattttat ctgagcgttt tgcgttcatt atttcagctc tcctttcttc
     3421 ccaatcatac ggatcacgat attaaagaat aatgacatca atagcaagat cagcgctaat
     3481 gaccataaca cattattttc aactgttccc atgattgtat ttccgattcc cattgttaag
     3541 atggacgtca atgtagaagc aggagtagta agtcctgttg gcatcaatgc tgcattaccg
     3601 atgaccattt ggatagctaa ggcttcacca aacgcacgag ccataccaaa aacgactgct
     3661 gttaaaatcc cgggcacggc tgctcgtagc acgactttgt agattgtttg ccatcttgtg
     3721 gcaccaagcg ctaatgaagc ttcccgatag tgccgtggta ctgcctttaa tgcatcaaca
     3781 gtcatcgttg tgacagtcgg caagatcata acgaacaaca caaatgtacc agctaagata
     3841 ccaaaccctg tcccgccaaa aatacttctg acaaaaggca cgataactga taaaccgata
     3901 aaaccataaa cgacagaagg aatacctaca agcagctcga tgaccggctg taagatcttc
     3961 gttccgtttt taggagagat ctccgtcatg aatactgctg caccaatggc aaatggcgtg
     4021 gcaacgatag cagataaaaa ggtcacaata aacgaacctg tgatcatagg caatgcccct
     4081 accattggtt tcccatttgc tccgatttgg cttggattcc aatctgtacc aaacagaaaa
     4141 tcaaatacat tgatcttatc agtaaaaaag gtggccaatc ctttactagc tacaaaataa
     4201 aaaatagata atacaaccaa cacgatcagc gcgatacata aaaaactgat gaattttcca
     4261 cgctgttcca tttttgaacg ttttgatttt gttaataata cttttttcac atcttccaca
     4321 gatgactccc ccttaatcag gtttaacata ctcactcagt gtacaaattg aacatcaatt
     4381 tggttttaat aacatgtaaa agtttagcta agtttgtaaa ttttgtgtaa agattactct
     4441 ttaacgatat ttccttgcca atcacgttcg actttcatat gagaaacagg gatataacct
     4501 agttctccaa cgatgttatc ttgtacttca tcagataaga tatagtctaa aaattctttt
     4561 gtcagttttt ttggatgatc tcttgtgtac atatgctcat atgcccaaat cgtccatttg
     4621 ttcgttgtca cattttcatc tgtcggttct acaccgttaa cattcaacgt atctacatca
     4681 tcggaaacat aagaaaaagc aacatagctg attgcaccgg gtgtgtcggc aacgatctgg
     4741 cgcaccattc cgcttgaatc ttgttcttgt gcctgaacag ctgtttctcc gtccaatacc
     4801 catttttcaa acgtgctacg ggttccgctt cctgaagcgc ggttcaatat aactactggc
     4861 tgatcttttc caccgacttc actccagttt ttgatctcgc ctgtaaatag ttttttcagg
     4921 ttttccatac tgatgtcttt tacgccaata tccttgttca caataggagt gatcccaaca
     4981 actgctactc gatgatcgac taatgctgag gcatcgatcc ccgatttttc ttccgcaaac
     5041 aaatcagagt ttccgatgtc gactgctcct gactgtactt gagacaaacc agttccgctt
     5101 ccgccccctt gtacattgat gaattttcca ggattttctg tctggtaagt ttcagcaact
     5161 gtttctacta aaggctgtaa agcagaagag ccaacagccg taatggattc tcctcgatca
     5221 atccactttg aacagcctga taaaagaatg gctaatccta ttattggtaa gaataattta
     5281 agttttttca ttagaaatct cctttaatcc taaagtatgt ttattttatc attttcatta
     5341 cttgatgaaa acgaatcaaa atcattaaat aacaaaataa attctcataa ttgtaaaaaa
     5401 aactatctca ttaaaagttt ctatgctata ctggctttga aatgtgtctc ttttaatagc
     5461 atatattagc ttatttcttg ctttccccaa aaaacaagag ataccccaaa gttaatttcg
     5521 ctcatgcgag acgctttctc catttctgac gtactctcag aaatggcccc gttagaaaag
     5581 gacacgagat ctccatctgt tgatggttct catgtccttt ttatgtttta aaggagcggc
     5641 gaaatcaatc ttgctaatcc ttctttcatc ttgatgatta ttcctcggtt ttcatacatt
     5701 tcttttgtga gatgcgtaga aattttcgag tcttcaaaaa aagcttttct tacacgtgaa
     5761 gccattcgtt catcataaac cagtgtattg acctcaaagt ccaagcgaaa tgaacgaaca
     5821 tcgatatttg ctgagccaac tgaaacaatg ccaccatcga taatcatcgt ctttgcatga
     5881 ataaatccat tctcatatgt ttcgatcctt gctccatact cgatcagctc tgcagcgaaa
     5941 gaatacgttg cccagtaaac gagaagatga tctggtttgt taggtatttg gatatgaact
     6001 tttacgcctg aaagaagagc caacttcagt gcttcatgaa tcgatccatc cggtatgtaa
     6061 taaggcgttt ggatcaatat ctcccgtttg gctaacgaaa tcattttcaa ataagtcatt
     6121 ttgatttgtt catgttcggt atctggtccg cttgtcacga tttgaacagc tatatcacca
     6181 gtagtgtgaa tggacgggaa aaatttggga gaatagccta cttcttttgt atgctgagaa
     6241 ttccagtcca tcaaaaaacg gttttgcaag gtatacaccg cttctccgta aatacgcaaa
     6301 tgattgtctc gccaataacc aaatttcttt accaatccta gatattcatt tccaacattg
     6361 aacccacctg tatatccgat tttcccatca ataactacga ttttacgatg attgcgataa
     6421 ttgatccgag gattaatata cggaacaaat agaggaaaga aaaatgcgac ttctccgcct
     6481 gcttttttca aattctgaaa aaagctggaa gaaacttgtg tggaacccca tgcatcaaga
     6541 aggactctga cttttacccc tcgtcttgcc gcctctgtca atgcatctcg aacttcttca
     6601 cctaatgcat cactgcgata aatataatac tggaagtgga tatggtcttc tgcctgcttg
     6661 atatcttcaa tcagcgcatc gaatttttca cgaccatctg taaataaagt gatttcattt
     6721 ttagtcgtat agagcgtact ttcaaatacc gtgagcatat agatcaattg tttcacatct
     6781 acttgtccag taggcggatg aggataaagc cctcgcatca atgcctgttt gtcttcttca
     6841 atttccaggt tcttaccgat tttcccttgg atcttcaaat caaagatttt ctctttggaa
     6901 atccctcttc ctaaaaaaat atataaaata aatcctacaa ctggaatgaa catcaaaacg
     6961 agcagccatg cccatgtttg agcagtttcc tttctttctc gaaaaacgat gataaaggaa
     7021 agcaaaatat tcataatcaa aaggatcgta aaaaaattgt caacgataat tttcacggct
     7081 tcacctcttc ttctaccaac ttaagaatac ccaagttgtt ttttaaagta aagggaaaaa
     7141 agccgcttcc ttctattctc ctagccaata aaaaaagaaa gctggaacga aactgtccaa
     7201 ctttctcatt ttatttat
//
LOCUS       DAZKMB010000126         6810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000126 DAZKMB010000000
VERSION     DAZKMB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.129"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            527..721
                     /locus_tag="ACG8V3_002460"
     CDS             527..721
                     /locus_tag="ACG8V3_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300937.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            912..1161
                     /locus_tag="ACG8V3_002461"
                     /pseudo
     CDS             912..1161
                     /locus_tag="ACG8V3_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1405..2235)
                     /locus_tag="ACG8V3_002462"
     CDS             complement(1405..2235)
                     /locus_tag="ACG8V3_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0300938.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2443..3777
                     /locus_tag="ACG8V3_002463"
     CDS             2443..3777
                     /locus_tag="ACG8V3_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0300939.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3818..4729
                     /locus_tag="ACG8V3_002464"
     CDS             3818..4729
                     /locus_tag="ACG8V3_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0300940.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4791..5579
                     /locus_tag="ACG8V3_002465"
     CDS             4791..5579
                     /locus_tag="ACG8V3_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0300941.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            5576..6192
                     /locus_tag="ACG8V3_002466"
                     /pseudo
     CDS             5576..6192
                     /locus_tag="ACG8V3_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            6194..6712
                     /locus_tag="ACG8V3_002467"
     CDS             6194..6712
                     /locus_tag="ACG8V3_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0300942.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2044 a         1184 c         1367 g         2215 t
ORIGIN      
        1 ttattttata ataaagatat gatgactaat ttagctcaac tattccagcg tcagctgaag
       61 atattgcaat tcccgattcc ttaatcattt atctggatga gacagaaagt gaagttgcaa
      121 cagatgtgca tgtagttgag gataaaaatg atagtttaaa aattaatagt tcagatactt
      181 tactaagagt aagtcctatc ttttcagctg ttatttagat gcaagtagca actaagttag
      241 gtagtactcc tgcaatggat aggtatttgt gtgttcgata tataagtggt cgaagtatca
      301 aggttatgta tactggatgg gagatagaag agttaaaaat tggaggtcac cgccacaaat
      361 ggaatttaga tttagaggta ccttagattt ggtatagtta ttaaacacaa gatggcctgg
      421 tttgtaggga aagtcgaaga gtatttttcc acttctgcgc ccctttaatt tttctagtaa
      481 tgccgtgaat ttgtgattaa ttggaaatat gaagaaagga gagtctatga aaacaacact
      541 agttctattc tataaaaagc atccttattt tacacttctg ataaatatat tgttggctag
      601 tgttatcggg atttctgttg aatatctaat aaataaagat ttcataggtt cttgttttta
      661 tacggcttta tttcttggac ttcttgaagc attttccatt tataaaaaat caaaaaaata
      721 aaatagattg ccgtaagaga tctttttcat ttcatttttt tctgaaaacc cgttgtttct
      781 gtcagtgaag cagcgggttt ttagatttat tgtctttaga cctaattgag cgcgccgagc
      841 gattgaaata caaaaccact tgctatgcgg gtggagcaat aaagggaaat agtttagccc
      901 gtacaaagta gatgtgtaaa taccacattg tattcacacc aaagtataga agaaaaataa
      961 tttataatca atatcgagcc aatattcagg agatcatcaa gagattgtgc aaatctaaaa
     1021 tataaatttg ggtatcgaca cttttgggtg gaaggatact atgtgacaaa agtaggacaa
     1081 aatgaagcga ccatcaaaaa gtacattcaa gaacaagaaa aacatgatag tgcgttagat
     1141 aagctgagtg tacgagagta agacgatcct tttaagggct aaggagtagt acataaacct
     1201 tttaagaggt gggcaaaact ggcaaaggca atatggcttg aacataaaat atgaaagcca
     1261 gcgtctttag gcgctggccg gtgatttgag cttatagctc tagagaaaac cacccttttg
     1321 aagggtggtt atgattaatg actttagtca gttttgctca caaagcgtgt ggaacaaaaa
     1381 acgggtgttt ctacgggtag atatttattt cttgaatgga ggtgtcccat acatttttgg
     1441 tgttactggt tctaattttg gtgcagcgcc ttcgatctta ggttctttca accattgata
     1501 ttcgccttcg ccatccagtg ctttgccact agcccaagaa ccttcactac tttggtctcc
     1561 ctctgagaag ttatagaagt cataagcaat gtcttttcgt tctttgtcac gcgggaaagt
     1621 acttgggact aagattgaac cttctttttc ttctaattct ttcactgcac attcccatag
     1681 attttgatga tagctatctc ttgcaaggag aacagacagc atatctttta cgccacgatc
     1741 atcgatcatt tcgtaaatac gtgctacttg aagtcttcct tgtgattcgg catttaaatt
     1801 agctctaaag tctgctaaca agttgccact cgcaatgata taggcaccgc tccatggatt
     1861 gcccacgctg tcagcaggac gaggtcctaa gccagctacg atcgcgtgct gtggattcat
     1921 tccagagata atggcaccga ttgttggatc tttttgcatc gctgcttctt gatcttcaac
     1981 aggcgcaccc tctaataaac gagcaaccat tgtcgccaac atttccacgt ggccgatttc
     2041 ttctgttgca atgtccatca gcatatcttt atatttttct tctcctcggc agctccagcc
     2101 ttggaacagg tactgcatag ctacactcat ctcgccatat tggccgccga tcagctcctg
     2161 cagatatttc gcgattaatg gattcggacg atctggtttt gcttcaaact gcaattcttt
     2221 ctggtgtcta aacatcaaaa ttcctcctaa tctattttta atacattctc attctgctac
     2281 gaaagattaa aaaattcaaa gaaagcgctc gcgaattata taaagtattg gtatcattaa
     2341 taattttttt agaatcatac cttataaatt gttattttca gaacaaaatg atatcgttat
     2401 attaaaaccg gcatcggtta cattaaaatt tggaggtaaa atatgaaaaa attattgttt
     2461 acagggttgc tggcaagttc agcattgttt ttattaggag gatgcggatc aagtaatgca
     2521 agcgatgcca gttcaagtgc agcaaattct tctgaaaaaa caagtgatgg aagaaaaaat
     2581 attgatttct ggtccttttg gggatccggt gcacgaaaag aagctatcga agaaatcatt
     2641 gaggatttca atcaatcaca aaacgagatc catgtaaatt ataagtatca gccatggggt
     2701 gacatctgga ctaaatcttt atctgcaata acagctggaa atgcacccga tgttattgta
     2761 caggacataa attccgttgc ccaaagagca gaagcacagc aagcaactaa tctctccaaa
     2821 tatgtagaaa aagacacttc taaggatttt tatccgcagc tttgggatac agtagaatat
     2881 gaaggtgatc cttatgcgat cccattcaat acagatacac aagtcatttt ttataataaa
     2941 aagatattta aagaagctgg aatcagtgaa caagatctac cgacaacttg ggatgaatta
     3001 gaagaaacag cacgtaaact agataaaaaa gaaggaaaag aatttacacg catcggtttt
     3061 tatccattat ggaatttagg ggctgatgta tgggcgctaa acgcagataa cgggactagt
     3121 tggtttgaca aagatgaaaa agtcaagatt gatactaaaa ataaagtgga agccttggag
     3181 tggatccaag actggcaaga ttattatggg aaaaatacga tcaataaatt agaagccgaa
     3241 tttgggtctg gtgtatctga tccatttatt tccggattag tagccatgcg tgcccaaaat
     3301 atcaattatt actctagttt gatggaaaat gcaccggatg attttgattt tggcgtgatg
     3361 cagctcccag aaaaagaaga aggttcaggc cattggtcgt ggggtggcgg attcgtcttg
     3421 gaagtaccat acggagcaaa agatcctgag gcctcttatg aattcatcaa atatctttct
     3481 tctccagaag tacaagaaaa attcggcgaa aaaagttttg atatcatggc gagcaagaca
     3541 gccaatgaga atttagtgaa taatgacaac ctaacggata aaggaaaaat gatctatcaa
     3601 atggcggacg aaaactttaa aaatacggtg atcacaccag ttcctttaga tgctccggat
     3661 tacacgacat tagtgaatga acagattgac cagattcttt taggaacaaa atcaccaaaa
     3721 gaaggattgg cagatgcaca aaaagcagta gaaaacttag tcaagcaaaa tcaataatcc
     3781 atttttgact gtctaggtac agaggaggca agaaaatttg aagcgtttat ctatccgaaa
     3841 gaaaaaagaa gcaagatggg cctatttatt tatctcgcct tttattatcg ggttcttggt
     3901 atttatgcta gggcctatgc ttttttcagt tattggtagt ttcaccgatt ataatttgac
     3961 atccaagatg aatttcattg gtctagcaaa tttcaaaaga atgttcgttc acgatgattt
     4021 attttggaaa tcgttataca acaccgttta tttcgtcatc ttcaatgtac cgctgacgac
     4081 gattttcagt gtgtttttag ccacagtact caatcaaaaa atcaaaggaa tcagtgtatt
     4141 ccgtacagct ttctatttac cggcagtctt atcaggagta gcagtctaca tcttatggat
     4201 gcagctgctt tctccatctg caggattgat caacacattt ctgggctggt tccatatccc
     4261 tggaccagct tggctgtttg atccacaatg gacgaaacct gcgttgattt tgatgaaaat
     4321 atggagttct ggaggagcaa tgctacttta cttagctaca ttacaaaatg ttcctagatc
     4381 tttatatgaa tcagcagaaa tcgatggagc aggcgtttgg ggaaagttca aaagtattac
     4441 tttgccatta atcactccta ttatttttta cgatgtgatc acctctacga tcggttcttt
     4501 ccaaattttt caagaagctt atgtcatgac gaaaaatgga acaggcggac ctgcaaattc
     4561 tttgcttttt tataatctgc atatgtggaa caaggcgttc gtagcttttg atatgggtta
     4621 tgcgatggct atgtcaatga tcctgtttct gatcgtgttg gttctgacgt ttgtcaattt
     4681 gaagcttgca cctaagtggg tctattattc tggaggtgac ggcaaatgat ggattatttc
     4741 aaaaagacca gtgtgaatcg gcaaacaaaa aaatactaag ttatgtcctc atgacagtaa
     4801 taggaatcat tttgatcatt ccattgcttt ggatggtgtt tacttcattg aaaccaatgg
     4861 aagaaatcgt tcgttatcct ccgacctttt tcccagaaaa aatcgtatgg gaaaactatc
     4921 tggatacgat tgccgccttt cctttttggc gctatgcaag aaatacatta ttcatcactg
     4981 tgctagtagt gatcggtaat gtcctttcaa attcttttat tgcttatgga tttgcgaagc
     5041 ttgattttcc tgggaaaaag ctgatgtttg ctttggttct ttctacgatg atgatcccgg
     5101 ggtttgtaac gatgatccct caatatgtat tattctccaa gatcggctgg gtaggtactt
     5161 atttgcccct gatcgttcca tcgttcttcg gcaatgcgtt caatatattt ctgatgcgtc
     5221 aattttactt atcgatcaat aatgaattga tcgaagctgc tgaaatcgac ggagcgaatc
     5281 atttgtatat ttggagtcat ttgatgcttc cattgacgaa acctgccttg attaccattg
     5341 cgatcaattc cttcaacgca gcttggaatg atttcttagg tcctctgtta tatatccaag
     5401 atcaagaaaa atacacgtta cagattggct tgcaagtttt tcagaaccaa gcaacgacac
     5461 aatggaatta tctgatggca ggagcaacac ttgtcttgat cccgacgatt ttgctgttct
     5521 tttttgcaca acgttatttc attgaaggga tggatttgac aggaggttca aaaggatgat
     5581 gaatcgatcg attggtaaaa aagccaatga ttatttctta gaaatcattg aatggattcc
     5641 acgtcttatc catttacagc tactatggtt cctgtgcgtc ttgcctgttt tcacattagg
     5701 aactgcaagt cgaacagttc tgtatatgat ttatcattac aaaaaaatga agagaaaaag
     5761 ctcggttcac tattctggaa aaagtttcgt gagaacttct ctctttatgg aaagcaagat
     5821 agtttagcaa gtgtttattt tcttcttcta ttgatcgatt atcagatttt ccgctattgg
     5881 ggtgggggaa ttggctatac attgatgtat actacgttgt ttttgcttct attaagcgcg
     5941 cttctcttta gttacaaaat cttgcttcaa ctggaacaaa aagaagcttc ttggattact
     6001 gcatttttct tgttttttaa tgatttcaaa agcgcgcttt tctatttggc aggcacgttg
     6061 atccttcttg tcgcattctg gtttgcagga ccaatctatt ttgctttatt aggtttcagt
     6121 ttcttgtttt attttcaagt cctgcttttt atccatcgta cgcaagaaaa ggttcttaaa
     6181 aaggaggaat aacatgttgc gtttgattgc taaaatcgat gatagtatta tcaacaagtt
     6241 cgccaagcaa cggaaaacgg atagtcgaaa aatcaatttt ttgaaattta tcagtaatat
     6301 ctctaatggt ggttttattt ggttgctcct tgctgctgtt ttagtcttga tacctgatac
     6361 tagagaatat ggtctagcca ttcttatcgg cagtttgatc tatttttttg ttggtttgct
     6421 ttttatcaaa aaaacgacaa gacggaaaag accttatgaa aaaaatcgtc ggcttgtcct
     6481 ttacacaagt aaaccgaaga cttcttcttt tccttcagga catacagcgc tagccttttt
     6541 atcagtagct gcgttgttcg aattaagtcc agttgcagga atattagcct ttttatttgc
     6601 ttgtttgatc gcttattcga ggattttttt acttttacat tatcctactg acattttagg
     6661 aggagctatt ttaggaagtg ttattggcat cctatgcgct gccgttatct gaattaattg
     6721 tacgaataaa gcatgagtgc gtaaatgcgc atttgtgctt tatttttttg tttcgaggaa
     6781 agcgtagttg tttattcatt tgtcttaata
//
LOCUS       DAZKMB010000127         6738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000127 DAZKMB010000000
VERSION     DAZKMB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.130"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..910
                     /locus_tag="ACG8V3_002468"
     CDS             20..910
                     /locus_tag="ACG8V3_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0300943.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(983..1807)
                     /gene="nadE"
                     /locus_tag="ACG8V3_002469"
     CDS             complement(983..1807)
                     /gene="nadE"
                     /locus_tag="ACG8V3_002469"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0300944.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1819..3291)
                     /locus_tag="ACG8V3_002470"
     CDS             complement(1819..3291)
                     /locus_tag="ACG8V3_002470"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0300945.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3928..4290)
                     /locus_tag="ACG8V3_002471"
     CDS             complement(3928..4290)
                     /locus_tag="ACG8V3_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0300946.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4513..5265
                     /locus_tag="ACG8V3_002472"
     CDS             4513..5265
                     /locus_tag="ACG8V3_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0300947.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5405..6328)
                     /locus_tag="ACG8V3_002473"
     CDS             complement(5405..6328)
                     /locus_tag="ACG8V3_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0300948.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6343..6615)
                     /locus_tag="ACG8V3_002474"
     CDS             complement(6343..6615)
                     /locus_tag="ACG8V3_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300949.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2053 a         1348 c         1154 g         2183 t
ORIGIN      
        1 cttttaagag gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga
       61 agcattagct agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa
      121 tgtcaaagat ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa
      181 aaagctagag gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag
      241 ccttcgtgtc gataaaatcg atattagttt tccagaaaaa atattcaatg cttttgatac
      301 gaaagaaaca tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg
      361 tttagctacg atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc
      421 tcgaaggatg gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc
      481 taatttttta aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc
      541 tgaatttcct ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt
      601 ggagctagga acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc
      661 tgattggtat ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa
      721 acacttgacg aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa
      781 agagcaagac acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg
      841 ctgtacttta tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta
      901 ctacagctga tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg
      961 gacggctgtt tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat
     1021 gtcgtttatg ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa
     1081 cttcttttcc ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat
     1141 ctgcaatcat tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt
     1201 ccggtgcatt caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg
     1261 gcaaaatgtc ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag
     1321 catgatctgt ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca
     1381 tacgttgacg tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc
     1441 ttaatacttg tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg
     1501 gctggataaa gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa
     1561 gacgaacagc gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg
     1621 ttagttgcgc caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa
     1681 aagttttaag aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta
     1741 tttcttcttt tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca
     1801 ggctcattta ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg
     1861 ttccatgata gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg
     1921 cggattcaag tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc
     1981 tttgatttcg tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg
     2041 taaaactggg cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg
     2101 gaacatgaag atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc
     2161 ttcagatttc ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt
     2221 tactttttct gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc
     2281 gatcgatact agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt
     2341 tgtcccaaca ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc
     2401 ttcatccaag tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg
     2461 ttcccgaaca cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa
     2521 gttgattttg tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa
     2581 cgtatcataa gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa
     2641 tgcttcataa tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg
     2701 tatcccaaat atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc
     2761 cgctctagtt ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa
     2821 tagtggatct tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg
     2881 gaaattgacc atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc
     2941 cacttggatc aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt
     3001 tgctttaaat tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc
     3061 acgcaagtaa gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg
     3121 ttccaatcct gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc
     3181 aaaaacagca tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg
     3241 atatagatcc gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta
     3301 ctcctcttgg caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga
     3361 aatcttccat tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac
     3421 tccatctgct aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta
     3481 ttttattcaa tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata
     3541 aaataagtag attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata
     3601 taaccaaatg tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt
     3661 tccttttttg cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt
     3721 tggaaaggcg aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc
     3781 acacgttttt aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat
     3841 ggcgagacat caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt
     3901 taccataaag atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat
     3961 cactacaata tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt
     4021 cactcctaca tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata
     4081 gtcgatttct tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc
     4141 atatgtgtct accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat
     4201 agccgtattc ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt
     4261 actattcaca ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta
     4321 gatccaatct taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa
     4381 cctttaaaga caaaaggatt taccttttat cacttaatag tataacataa tttcatgaga
     4441 aaaaaataca attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca
     4501 ggaggaaaaa taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg
     4561 agaaccttgc gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca
     4621 ggttatcgga tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga
     4681 acaatggcat tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac
     4741 cagaaagctt taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc
     4801 ctcttgacaa ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac
     4861 aaagaaaaat ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga
     4921 caagagattc gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg
     4981 aatatggata aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat
     5041 ctcactcttt acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct
     5101 cataagaagt ggttatctta cagctggcct gaatatcagc caaaagcgca taaaggacta
     5161 ggaatgatgt acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat
     5221 ggtgcagcaa aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc
     5281 aacaaaaaag tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt
     5341 ttatgctaac ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat
     5401 atattcatcg ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa
     5461 acaattgaaa tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa
     5521 gaagctcgct gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat
     5581 agctttgcaa gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa
     5641 gctctttaaa aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa
     5701 caagtgattc atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt
     5761 tataacttag cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct
     5821 tcaaataagt aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct
     5881 gttttccctt gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat
     5941 catctagtgt attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt
     6001 cttcaagata ccttcgctct ttttctttct gatagcccgt cggtaaaagt gtcactttgt
     6061 aatcataatc aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat
     6121 tggtataagg cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat
     6181 ttgcattagg gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct
     6241 catctacata tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga
     6301 gctttgcgat atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc
     6361 ttgtaaatag tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg
     6421 cgaaaaactg atccgctgcc cgccccaata tatatctttt gtcaaatttt cgatttcatt
     6481 cattggtatt tcttttacta tattcctatt caacccttga aaactgtaca acgttaatac
     6541 ggatcccttg aactcgacaa acgcataaaa ataattgttc gttgtgccat ctgttatctc
     6601 tacataagta ttcattgcaa gccctccttt tgatactttg agtatagtgt atgaaacgcg
     6661 tctcatagca aggagtttgt tttgccaaag aaaagtttta ttcctctttc attattacca
     6721 aagtctctta ttttaaat
//
LOCUS       DAZKMB010000128         6644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000128 DAZKMB010000000
VERSION     DAZKMB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.131"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8V3_002475"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8V3_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8V3_002476"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8V3_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0300950.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8V3_002477"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8V3_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8V3_002478"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8V3_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0300951.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8V3_002479"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8V3_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0300952.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8V3_002480"
     CDS             3582..4271
                     /locus_tag="ACG8V3_002480"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0300953.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8V3_002481"
     CDS             4285..5787
                     /locus_tag="ACG8V3_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0300954.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8V3_002482"
     CDS             5800..6276
                     /locus_tag="ACG8V3_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0300955.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8V3_002483"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8V3_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZKMB010000129         6614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000129 DAZKMB010000000
VERSION     DAZKMB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.132"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG8V3_002484"
     CDS             complement(105..629)
                     /locus_tag="ACG8V3_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0300956.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8V3_002485"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8V3_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0300957.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8V3_002486"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8V3_002486"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0300958.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG8V3_002487"
     CDS             complement(2351..3031)
                     /locus_tag="ACG8V3_002487"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0300959.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG8V3_002488"
     CDS             3157..4026
                     /locus_tag="ACG8V3_002488"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0300960.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG8V3_002489"
     CDS             4052..4567
                     /locus_tag="ACG8V3_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0300961.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG8V3_002490"
     CDS             complement(4614..5177)
                     /locus_tag="ACG8V3_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0300962.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG8V3_002491"
     CDS             complement(5232..5411)
                     /locus_tag="ACG8V3_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300963.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG8V3_002492"
     CDS             complement(5488..5997)
                     /locus_tag="ACG8V3_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300964.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG8V3_002493"
     CDS             complement(6093..6473)
                     /locus_tag="ACG8V3_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0300965.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         1860 a         1395 c         1172 g         2187 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aaga
//
LOCUS       DAZKMB010000130         6609 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000130 DAZKMB010000000
VERSION     DAZKMB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.133"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1570)
                     /gene="pglX"
                     /locus_tag="ACG8V3_002494"
     CDS             complement(<1..1570)
                     /gene="pglX"
                     /locus_tag="ACG8V3_002494"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0300966.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKK"
     gene            complement(1634..5305)
                     /gene="brxC"
                     /locus_tag="ACG8V3_002495"
     CDS             complement(1634..5305)
                     /gene="brxC"
                     /locus_tag="ACG8V3_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0300967.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(5330..5905)
                     /locus_tag="ACG8V3_002496"
     CDS             complement(5330..5905)
                     /locus_tag="ACG8V3_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0300968.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(5905..6504)
                     /locus_tag="ACG8V3_002497"
     CDS             complement(5905..6504)
                     /locus_tag="ACG8V3_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0300969.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         1943 a         1253 c          880 g         2533 t
ORIGIN      
        1 atttcttatc cattttatta agataaggcg gattagtgac taccgctaca tatttatcaa
       61 ctagcatttc agccacgtca agaatgtgca agtatttttc ttgtatttcc ttcatcccat
      121 aaagaatatc catattcgta aattcgttga attttgatat atactctctt acttttccta
      181 agtattcttt agttattgcc tgattatttt tatcgactaa cttaatgatt gatccaagtt
      241 ctttcccatt ttcaaattga tcaaaaatta actctttgta ttcattccag cgttcactat
      301 acttatcatt gttcaattca ctaatacgct cttgatactc gtctgtcact ttaggagaat
      361 caataaactc tcttaaattc ggcgatagac tttttttcaa aaaaagacga tcgtattgac
      421 gccctttcat cataattgca aaataagaca attgatacgc tcttttatcg atttcaagac
      481 catataaatt gtattcaacg attgcacgtg cagcatcacg ctcagaatat cctaacgtaa
      541 gataaatttc catcaataca tcgaaggcgt aaactaaaat atgcccagac cccattgcgt
      601 tatcgataat ttttagttct tctggtttta aattacttac agatgttgct tgttcaaaag
      661 catatttatc tggaattaag tatttcaact tatttcttaa ttcactattg ggataatgat
      721 ctaaccaata ctttcctaaa ctattgtcaa ccatataacg aacgacccaa tctgtggtaa
      781 atagttgagt tgctgccgga atatcttgtt ttttgacaat ccctttataa atattcacca
      841 cttgattatg tggctcacta ttatagtact gatacaacca cccaataatc tctacctgtc
      901 caccagcttg aacatagaaa tcatcctctg gaatacttaa aagatcagcg attacaccat
      961 cttttttaat ataagaagct gtaaacaaaa gttctgtata atcttctact tcttcaaata
     1021 gctcaggtaa gtaatcattc aattgattac agatttttat aaaaatcaat tgatacagct
     1081 tgtccattgc ttctgctgaa ttatctgttt tccaagttct gacttgttgc tgttcattac
     1141 tagataattc ttcaaagata tcggcatcat aaatatctgt aataatatct ggctcaattt
     1201 tcccacttat ttcactagat aatacacgat acgtgtcagg taaataatca tttacctcca
     1261 taaaccgaat ggcaatcaat cggttaaacc acgtataagc aacctcttcg attaactctt
     1321 gaaaagcttc tgaataagaa gtgacagaag cacgttgttt tagttctgca atcaatttgt
     1381 ttctttgcgc tatttctttt ccttgaattc ttattcctga tttttcgatt tcttgtaaat
     1441 tgctatcaat tttcttcaca tcttcaattt ttgattcatc aattcctaaa cgttttgctt
     1501 tagtcattac ttcatcaatt aattttctac gggcattgac cgcgaaattc tttatcgctt
     1561 tcttatccat ttttctttcc cttctatatg taacctttga aaagacaggc acctttttat
     1621 gcctgtcttt tcactagaat tgaatcgtga tttctgtatt ttcttgaagt tgttccttca
     1681 gtttttgatt aatttcagct gttaactcat caatatctgc tgctgactta atagttttaa
     1741 catgaacgtt aagtgttctc attggtactg ttttctgttg aatagtaggt tttactatcg
     1801 ctatttcttc agcttttgct gcagagtcta tttcatcttt ttcagtttca cgaatcaagt
     1861 ttgctgcttc aatttgttta cgtgcttgtt cttcttgacg tctgcggttt tcttcattat
     1921 cgaaacgttg attaaagatc aaatactgtt ctgctacgtt taatgataag aaaacaagat
     1981 catctatagt attcgcttgt tcaatttttg ctttcagttc ttgaaattct tggtcaacta
     2041 ttggtacaaa ttgatcctta aaatcttttc cttctaataa tttaaatgtt gcttgttttt
     2101 cttccgttaa tttatttaat atttcctgac tttccaactc taacagttga ttatattgtt
     2161 caataaattc ttgaatcaac tcatttagtt tcatcatatc tgaataagga tcagcctttt
     2221 tcaaaatggt tttcatctca tgaatcaatt gatcaatttc ttggctagaa gtttgacgcc
     2281 gacttttttc gattaatcta attttatctt gtgcattctc ccagattttt ttctgagtac
     2341 tattttgctt gtggaaattt ttaattttat catatgtttc agcccagtcg aataattcat
     2401 cgcgatattt tttgattgta taaaaaaagt cttcaacatc tgacgtttga acaattactt
     2461 caagatattt ttttatttgc tcatattcat aagcactagg gtaggtataa gtgttttgta
     2521 aatcaaaata agagcgtaaa ttgtctaatt gaccttttaa atatttcttg aaattttcca
     2581 ttgtatgttc attgttattt tcatcaacaa tattacgact ttgaaacagt tctctaccga
     2641 tttcgtcaac caattttttt gcttttggcg aaatcaccgc gcttggttca atttttatct
     2701 tttcactttc tcgtcgtttt tgaataatca ttacaatact atttggatca tccatccgtg
     2761 aaatttgtac attgttataa agtaaatcaa ttttaccaga catgaaaagt ttagcgatgc
     2821 accattccac atcaagttct gtgaatccat acggttgttt tgtttcaaaa cgatcacgaa
     2881 ttgttttcat cgtaatcgtt aggtgatttc tcttttgatc attgataaaa ttgaaaattt
     2941 cctgaactgc ttgctcattt gatggatctt caataatttc atttttgctt tctttcacta
     3001 aatataagat atcttctttt tctttaggcg catcaatttg gtttaattta tggtatactt
     3061 cttcagccat tttctgaagt gctgattgga attttgcttt aaagtcggag cctgtcagtt
     3121 gaatttctgc accgttatag aaaaattttg cagatttcaa cagttcattt aattgatcgt
     3181 tgatacttgc acttagttcc tgtaattcac cagatttttc cgaacgaatc gcgtcaaagt
     3241 tctttactac actcgaataa gtcgttgtta aataaagatt cattctagca tacattgcta
     3301 tatcatcttt aaattcttta tctgatggca attctacgaa taataagtta cgggaactct
     3361 taagcgcaag ttctgcctct tgacctgcta aatcagagta tggtgaaata aaattaactg
     3421 tcatttcagc atttccagct tgcttgtatg gacgttgatc taacacttgt gcataagtaa
     3481 acgtataccg gtttttaaat ttatttccac ctgacatgcc taatttttca taggcaaaac
     3541 tatttcggtc tagaacgccg tcaaaaattc gtttagatag ttctacgatc aaatcactat
     3601 ctgtaatatt ttgattttta atctcacgag taatttcctg ttcttcatca gtttggaaaa
     3661 tataactatc taagttcttc cgcacaaagt tttccttaat taaaaggtcc aatgcttctt
     3721 ctactcgttt tttagtttcc aggcggtctt cgttgatatt tcttatcatc aagctcgtaa
     3781 tattttcaac atttggtttg atggcttgat caacatattt aatcatgaat aaaattcgta
     3841 agacatttac gttaaagtta tctatatcag ttggtctttg aatatgagaa ttatttgtag
     3901 cttgtgtaat cactcggcta attgctgtat caatccaacg ttctaacgag taataaaact
     3961 gatctaaaga aaccaaactg gttgtatcat tttccttatt tcgttcagcg gcttctttaa
     4021 acattgctag taatgaacgt tcaccagacg ataagtttga tccttgatag ctgtgattac
     4081 gaatctcggt tagaatttgc cccaataaat tgaattgata gggaataaat ggataaactt
     4141 ctgcgtaatt ttcagcatct tggaagagct ttaattcagg actatcaaca aagagtaatt
     4201 tatttttcaa ttctgtttga acagtttcaa attgtaaggc taaagaatca ttggcaaact
     4261 cttttttctt caaaatacgt tctttaatta cttcatcggc atttgctgaa gataaactca
     4321 cagttgtggc aaaacgatcc ttgatcttag aaaaatcatt atcagaattg ttatcaaagt
     4381 ttgttacgtg atcaatcgct tcctgacttg tcactaatac ccaagcctta ccttcacaaa
     4441 ttcgacccaa atcttccaca acggtttgta actgcaaagt caactcacga ttaccgccga
     4501 tatattgtcc gacctcatcg actagaaagg caatatgatg gtctgcttct ttggaatcaa
     4561 gatagctttt aactagattt gcaaagtcat caatgcttat taaataattt cctgatgctt
     4621 tttctaaaaa attatcaata gcggcttcac tcataaaatc gattttaagt gccgcttctt
     4681 tgatatcatc ttggatccaa tcaaactcat ggcgctcttc ttgccatttt gccccattaa
     4741 tctcttcaaa agcagctttg aatgcatcaa acttttcttc tttaattaac cgacgttcaa
     4801 aatctgctag atgtggaatc gtgcttaatc cttgcatttc gttaaatacc tttaaaaaaa
     4861 cattcacgat tccgtttttg tcttttttac cacctgcatc actttttgaa tcaatattaa
     4921 acagaataac atctgtattt atttgtgagc accgttccat cgttgccaaa gtcagtggat
     4981 ctttgatttt cccatcttct ttgaagtaat caatggcttt cttgtcattt atttctcgat
     5041 ttgcgagcaa gtaagaaata atttttaaga aatgagattt acctgaacca aagaacccct
     5101 gaatccaaac acccatttta tctgttggtg tattgatact ccgttggtag gcatcaaaaa
     5161 ttgttttaaa gtgtttttgt agttctttcg taaccacgta ttcttctaat tcttgttgaa
     5221 cattttcatc ctttgcttga ccgaccttaa taactccttg aatatttcgg tcaattggtt
     5281 ttgcaaaaat atctttaatt agcatttaat ctcctacctt ccatcccaac taaattctaa
     5341 atgctcgata ataattatca tctttattgg tattaagaat tgtcaaactt aattcatcat
     5401 attcccctgg atacatcatc acaactggta cgcgatcaaa tacctgatgc attttattta
     5461 aaatagagtg cgctctaatt aatggatata attcgccaat tcctgtcagg aacacaactg
     5521 atttttctgg tgtatgggca tccacatatt caaaaaatac attttcttcc tcaggtaaat
     5581 ccattttcaa taaacgacca atgctttcca ctaatttttg taaaccacgt cgttcttcca
     5641 tcttaatcgc tttttcagtg aaaccgcgtt tttctagata ctctaacata atcacataga
     5701 gattataaac tttcacttca taattatcac caacagatgt tccagatata tcattaatat
     5761 aatctcttac aattaactcg tctttcggat cgtagtcaaa gatataatag cctacttcat
     5821 tacctaaacc acgattcaat aaaaaatctt ctttttgaat tacttcattc aacttttcca
     5881 aatgctcttg aatcgataat gacattataa aattcctccc agtgctagta caatatcttt
     5941 acgattcttg tcaactaacc attgaatcaa tcgtttatct aataatatcg gagtcattcg
     6001 tccatattct ttttcccctc tgactaatcc tgtttcaatt agattcgttt gataagcggc
     6061 aataagccgt ttaatcgtat aatctttcca ttttgaaata tcttctgaac ttctccgctt
     6121 ctctgtaaaa aaagaacggt aatcaactgg tgttaattgc aaatttcctt tacgtagatt
     6181 ttcagcatat acctcagtca taaattcatt aaaaagatca tcaatcataa ggatggaaat
     6241 caaattcact aacttttgat tatctatatt taactgattg aaaagttgca atccagcttc
     6301 atccagttga ctaactctcc ttttcacgtt ctgatagatt tctttttgac ggttactgct
     6361 cactgcaaaa aaaatatttt catgttcatt tttctcttta atttctatat ctgtatatcc
     6421 ttctagtcgt aattgaatat ataacttgaa ctctgaaaac caaaagcgtt tagaaactag
     6481 accagcagaa tattttttat tcaatagatt gcctcctaat catctataat tcaataagta
     6541 aacaagtgtt ttttgctcat cgttagcatt cattttttag tatctactat acaatacata
     6601 tttattata
//
LOCUS       DAZKMB010000131         6517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000131 DAZKMB010000000
VERSION     DAZKMB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.134"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..325)
                     /locus_tag="ACG8V3_002498"
     CDS             complement(<1..325)
                     /locus_tag="ACG8V3_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300970.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDE"
     gene            complement(322..1014)
                     /locus_tag="ACG8V3_002499"
     CDS             complement(322..1014)
                     /locus_tag="ACG8V3_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0300971.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(1020..1691)
                     /locus_tag="ACG8V3_002500"
     CDS             complement(1020..1691)
                     /locus_tag="ACG8V3_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0300972.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(1684..2025)
                     /locus_tag="ACG8V3_002501"
     CDS             complement(1684..2025)
                     /locus_tag="ACG8V3_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300973.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(2203..2901)
                     /locus_tag="ACG8V3_002502"
     CDS             complement(2203..2901)
                     /locus_tag="ACG8V3_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0300974.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2988..3458
                     /locus_tag="ACG8V3_002503"
     CDS             2988..3458
                     /locus_tag="ACG8V3_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300975.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(3501..3680)
                     /locus_tag="ACG8V3_002504"
     CDS             complement(3501..3680)
                     /locus_tag="ACG8V3_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300976.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3667..4005)
                     /locus_tag="ACG8V3_002505"
     CDS             complement(3667..4005)
                     /locus_tag="ACG8V3_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0300977.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(4021..4224)
                     /locus_tag="ACG8V3_002506"
     CDS             complement(4021..4224)
                     /locus_tag="ACG8V3_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300978.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(4239..5015)
                     /locus_tag="ACG8V3_002507"
     CDS             complement(4239..5015)
                     /locus_tag="ACG8V3_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0300979.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            5314..5634
                     /locus_tag="ACG8V3_002508"
     CDS             5314..5634
                     /locus_tag="ACG8V3_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0300980.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            5652..6083
                     /locus_tag="ACG8V3_002509"
     CDS             5652..6083
                     /locus_tag="ACG8V3_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0300981.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            6213..>6517
                     /locus_tag="ACG8V3_002510"
     CDS             6213..>6517
                     /locus_tag="ACG8V3_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0300982.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLK"
BASE COUNT         1934 a         1214 c         1014 g         2355 t
ORIGIN      
        1 tttcgtcaat aggggtattg cgtttttcgt caatagggta ttgattaatt cgtaggtacc
       61 tattgattat ttgattggta ccctctttgt aaatgatttc ccgattcaag tatccaaact
      121 taatcaaatc acttacccat cgcgatatgg tctctttatt cacaccatat aaatctgcaa
      181 agtactcatt gcctgcccaa caaaagcctc tttcattaca caaggccgtt atctctccgt
      241 ataacaactt agtatttggt ttaagtcttt tgtcgtacct tacgttggct ggtataatcg
      301 cataataact tcgatgttct gtcatttttc ccctccaata tttaactttt tgattgtttt
      361 ctggtttaat ttgatccctt tgatttgata tttatttttg aaattaatca cacctatttt
      421 gtgcttctct gtgtgatgga ttctgcagag tgctgcaaat gtgtactctg catgatcaac
      481 ttctttgcgc tttcgtcttc ctagcgcttt gtcaaagtga tcgatgtcag ctcctgtttt
      541 gccacagatg cagcagactc tttttgtgat gcatttgtag aagtaatatt cttgattcgc
      601 aggtaaaatc tcatagcctt ctttgaaagg aatatgatgt tcaaagatga aatctaagat
      661 gatatttgct aagacattgg catcactcac agttgtattc gattcgtctt tcaggcttat
      721 tttgcgccct gtgacacctt caaaacgaaa gtagaagaat tccttccaga agtccgttgg
      781 catgcctgta tcgataaaaa tatcgcctat cagcgcatag atgaagtttc gttgctgtac
      841 ggtgaaacgt ctaggatcaa taaaacgaat ttcaatgact cgatcaccat cgtagccgtc
      901 atacatcgtc tttagtcgat caatgttcac ttcctcattg atggttgcgc ttatgtcttt
      961 tcctttgaac tttttcagaa ccgctgaata tgaatcgatt aatggtttaa acactcatat
     1021 cacttcttat ctaattcttt tctcttagct gctattgctc gctccatcaa ggcacattgc
     1081 tcatagctta actgttcaat agtttcaacg ttatcagcta agagccctaa tttatctgtc
     1141 tgctcattaa catattcgat taaggttttg gtcatatctt tacccatctg ctcattgaaa
     1201 gcttctagaa tcgtctctag catgtttaat ttctttgtat cgattctagg tggtgttgga
     1261 atatcttccc cttgaaatac atataatccc agtccgtgta gagccaatgc tttcacaaag
     1321 catcgcttca atgagttatt gatttgcatt gcatttggtt taacaactgg ttggtttcga
     1381 taatctaaaa caggaaataa ttcggtttcc gtgtgtcctt taaccgttac tgagacagat
     1441 acataagtcc cagtttcatc cataagaaaa ggtttatatt cctcaacaag aaagtcttga
     1501 tgagttccag aaacaaccct gtagtgtttg tactcattaa tagttaccgt tgcctgtgga
     1561 tcattctttt tcataatctc ccacgcgtga gcccaagata aataatcaaa atttcctttt
     1621 ttcttgagaa ttttatttaa cttacgacta aaaagttttt caaagttcgt tgtccctttg
     1681 atttcactca tcaaattctg cctccatttc agcaatgtat ttcttacctg gtccgtaata
     1741 agagatatca atcaagttat ctctgtcgta ctcttctagc gcatcaatca agccatcttc
     1801 gatgacgtaa atatattcag gtttattcga atgcttcgat agatggataa gataaacatg
     1861 atcccaaata gttacatggt tgcctaaatc atcttgatcc caagctagtt cttcattcgt
     1921 caaaagattt cgtctgattt ttcgaccact tgtttcctca attttcggct tgccccaatc
     1981 aggatcaatc aaatattgat ctagagtgga aagttctttt tccatatgct aaaatctcct
     2041 tatgatgtgt tttctttgtg actctttgct tgccggcgga gtcacttttt tatttgttgc
     2101 catgcttttt gcttgtcaat atgttgttgg cttaggatga ttggtttata gtatttccac
     2161 cagcaattag caattgccgt ccctattctt agcgcttcag ctctattcat tgtcatcacc
     2221 aaaaagtctc tgttgtctgt tcagttgatc aatttccata cggatcgcag tttctggcaa
     2281 ccacatttca ataaatgaaa cagcatcatc gaatctctta cgaggtaact cgccatatct
     2341 tgggattgaa aaggtacgtt taaattcaga ccaaaatttt gagaatactt ttttgctgat
     2401 ttcttcataa gctcggcttt cttttccccc tagaactccc ataactttca tatttccttt
     2461 ttgcttaatt tcaaactctt gttgtccgct aattcgcata gtatctttaa gcatggaaac
     2521 atctttttta acatctttca tttcttctaa ttgatagatc atcatatctt caattgtttg
     2581 aggaacagta ttcttgcgaa taacatcttc catttcgttg aatgcttcaa tgtatttttg
     2641 tttgaagtag atagctttct ttcctgtaaa ccccatagcc agcaagaaaa atccatctct
     2701 actaatgaag aaaactcgtc gatttctgcc atatgaatct ggttcattac cttctacaaa
     2761 catctgtcca aaattggaca catctttttt tagtgcatca atatctctta aaacatgttg
     2821 atgttttttc tcgaagcttt ctgccacttg taagctcgta gtcacagctt ctttattttt
     2881 caaaattact aattcttgca ttatttcttc tctccttttt ggtataattt agttaaaaac
     2941 tggtggtgtt tattttgaat ttttttcact taacaatttt tcaatggatg tcaataataa
     3001 gttttatatt gagtacgttt ttaacaggga taaaaataaa aggaatgcgc cctcaactag
     3061 aagttgaact taatgcttct tacttcgctg cagatatgat ttatacaaaa gtaatcattt
     3121 ctaactattc aacagaacca gctatgttag ttaatttaga actttcatcc gctaacttaa
     3181 accgcagatg gtcagctact ccatttaaga aattaattgc taaaggagga tcagtcgatg
     3241 ataatagaat atactctgaa gctgtaccgc taaatattcc tcctaaaagt gctatatctt
     3301 gttaccttgc atttgaagta gggaaaatta attttaataa actccttaat caccaaacaa
     3361 aaatgatatt tactctaaat agaacccaaa tccacaaaat tgttgatatc aaaaatacaa
     3421 acttcccaat agagaaatta gtgaaagaat taaactgagt aatagtaata tacattctag
     3481 tttctctttt ctattcattt ttagtcagcc ccctcggttg gctttttcgc tctgtactca
     3541 gcttcagctt catcaagccc cataaaaatc caaaccatgt aaacaatcgt tcctatcaac
     3601 gcttgcctgc ttccccaaag tcctaaagcg tagatgatta gcggtgcgct gaacactagc
     3661 gctctattaa attttcccat ccgcttgcct ccttaagatt tccgaaaaat ttgtttctaa
     3721 aaattcaagt gttttacttc ttaaaaatag atatgtgtct cttccttcaa ctggataata
     3781 gacaaatcca tttttgtttt tctcgatatc gataatattt ctatatcttg ggttctttaa
     3841 aactctagaa gtaaaccaat catattttct gttaatccgt tctagcactt ctggcaacgt
     3901 catccatcta ccagtatcat cagctttttt taactcctca taatccactt gggagataat
     3961 tacatagcct tctggaattg ggatttttgc ttctagatat tgcattagct gttcctccct
     4021 ttacaattcg tacatagtga taatcgaatc tataattctg tttgcttctg cagaagtctt
     4081 tttaccgttt aaaattaaag ataagtagct tttgctaatt ccaaatcttt cagcaagcat
     4141 ggtgtaagtt aagaactttg aactttcgac atattctttg attttttctc tatctcgttg
     4201 agtgatttct gcaatgtcag tcatactaaa actcctttct aaccaatttc ctctaaatcc
     4261 atttgagggt aatatccttc tttttttagt aattcgtaga taaatagacg ccctttctgt
     4321 gtccatttgg tattcattac aattttagtg ccaccatcgg ctttcgggat ctcagttgta
     4381 tgagattttg tgtatccttg gttcatgtgt tttttgcaca ataaccattg gttaccgact
     4441 tttttctgaa tacctagttt atgaagtaat ttattcatct gttgtggaga catcccataa
     4501 tctgctgcaa tctgactaat tgttactgaa tctgtagaag ataatatgct atctaaatag
     4561 gagattttag gttcgtactc tgcaatcttt tgttctgcga ttaatcttcc agttctttct
     4621 tcttttagtt gagttgctaa ttgaatgatt gtatctggat taagcaaagc ttcttctact
     4681 ttttctggag ttagataacc tccatgtttt ctaattgctg gcaacacttc acttgttacc
     4741 caacgtttaa attttttggc agagggaagt tttgatttta agattaaact gtacaaacca
     4801 gattcgttga tgattgtcat ctctcttgat tgacctgagg tcgtgattcg cgaccccatc
     4861 ttatcttcca aatctacatg ccgcgacaaa gcatctttag tgtttgaata acccaaaaca
     4921 ctcgcaacgt ctttgcctac aaaatatggt tcatcattta ctaaaattgt ccgaacttcg
     4981 ttttgttcga aattaaaaat ttgcggtgtg ttcatatttc tcattccttt cttttatatt
     5041 cgtaaacaaa tttaacaact tttttctaaa ttcaattgac agtatctgag taatactcta
     5101 tactaagagc ataattaaat aagacataaa aacattgatt ttaaagcttt cttggcggtc
     5161 tgcatttaca tatcaatagt gttttttgtt gtctttttag ttgttaaatc tgtttacaag
     5221 atatagtata gctcattact cagaattagt caaattattt ctgcgtattt ttctaaactt
     5281 tttttgtaaa catttagaaa ggttgtttta tcgatgaata cctatgaaat aataaaagac
     5341 ttggcgaaga ggaaaaagat atctatacgg cagttagaaa ttaattttgg atattctaac
     5401 ggttatctag caaagtggaa aaataatact cctaattcag atgaattaag tcggattgct
     5461 gactatttcg atgtctctgt agactacctt ctaggtagag aagaaagaga aacccctaaa
     5521 catgtggatt tatcagaaga cgatactgtt ttttcttttg atggaaaaga aatatctaag
     5581 gagacaatgc gtaaagcgat tgcaattgct aaagctttag aggaaaatga atagttggag
     5641 tgatggtttg tatgtatttg aaattgaaag aaatgttgag cgaatacaat ttgaaagtag
     5701 tttatatgga gatgaaagag cctggtttct actatccaaa acccagaatc atttttttga
     5761 atgaaaatct atacggtgaa actgcagaag cctttcattt atctcatgaa cttgcacatt
     5821 tcagcgcctc tcattttgaa ttttcagttt tgtacgatac ttctacaact tttcactcaa
     5881 aatttgaaac agaagcagat aaaatagcta ttctaatttt attaaatatc tatattgaga
     5941 acgaactgac tgatgaatct cagtttaatc tcgaaaaatt tatggagtat tattctattc
     6001 agaataagct cagatacact tgttatgctg tctgccaatg ctattttaag aaaaaatatt
     6061 cttacgcaag gcaatatgta tgaatacatc aagaattatt aaaaaaaccg atttcagcaa
     6121 aacattaaaa aagcccgtgc tgcaacacgg gctctttcct catatatgag cttctacaaa
     6181 aaaaatcata tcacagaaat gaggaagaaa aaatggaaaa agagatttta ataaaatctc
     6241 caggaaaaac cttgattaaa gtaactgatg attctatttc aattattaga aaaggattta
     6301 taaatttagt taatcaaggt attaaaggag aaaaaactat cccctttaaa aatatatcag
     6361 ccgtacaatt aaaaaaacca ggtatgagta atggttatat acaatttacc ttgctaggcg
     6421 gaaacgaaag tcgcggaggt atattagcgg ccacgaaaga tgaaaacacc gttatgttta
     6481 caaaaaaata ttggaatgaa atggaaaatt taaaaaa
//
LOCUS       DAZKMB010000132         6346 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000132 DAZKMB010000000
VERSION     DAZKMB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.135"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8V3_002511"
     CDS             complement(99..1229)
                     /locus_tag="ACG8V3_002511"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0300983.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8V3_002512"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8V3_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0300984.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8V3_002513"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8V3_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0300985.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG8V3_002514"
     CDS             complement(3485..4552)
                     /locus_tag="ACG8V3_002514"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0300986.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8V3_002515"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8V3_002515"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300987.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8V3_002516"
     CDS             4880..5692
                     /locus_tag="ACG8V3_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0300988.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8V3_002517"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8V3_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300989.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1895 a         1300 c         1001 g         2150 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagata
//
LOCUS       DAZKMB010000133         6090 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000133 DAZKMB010000000
VERSION     DAZKMB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.136"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACG8V3_002518"
     CDS             complement(254..541)
                     /locus_tag="ACG8V3_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0300990.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACG8V3_002519"
     CDS             complement(561..785)
                     /locus_tag="ACG8V3_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0300991.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="ACG8V3_002520"
     CDS             complement(1240..1410)
                     /locus_tag="ACG8V3_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300992.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACG8V3_002521"
     CDS             complement(1425..1958)
                     /locus_tag="ACG8V3_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0300993.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACG8V3_002522"
     CDS             complement(1979..2923)
                     /locus_tag="ACG8V3_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0300994.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="ACG8V3_002523"
     CDS             3560..3646
                     /locus_tag="ACG8V3_002523"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0300995.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="ACG8V3_002524"
     CDS             complement(3767..4456)
                     /locus_tag="ACG8V3_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0300996.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4701..5615)
                     /locus_tag="ACG8V3_002525"
     CDS             complement(4701..5615)
                     /locus_tag="ACG8V3_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0300997.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="ACG8V3_002526"
     CDS             complement(5597..5977)
                     /locus_tag="ACG8V3_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0300998.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1612 a         1125 c         1027 g         2326 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagctcac taaagggctt ggcaagctct
//
LOCUS       DAZKMB010000134         6064 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000134 DAZKMB010000000
VERSION     DAZKMB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.137"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..887
                     /locus_tag="ACG8V3_002527"
     CDS             42..887
                     /locus_tag="ACG8V3_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0300999.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1168..2388
                     /locus_tag="ACG8V3_002528"
     CDS             1168..2388
                     /locus_tag="ACG8V3_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0301000.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2385..5105
                     /locus_tag="ACG8V3_002529"
     CDS             2385..5105
                     /locus_tag="ACG8V3_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0301001.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5089..6033
                     /locus_tag="ACG8V3_002530"
     CDS             5089..6033
                     /locus_tag="ACG8V3_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0301002.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2270 a          955 c         1185 g         1654 t
ORIGIN      
        1 cttacaataa gcagtgaatt tataatcaac gtataggaga catggatgaa atgaaactaa
       61 ttggtataga tttagacggt actcttttga attctgacca gagcattagt caaaaaaatg
      121 caagaacaat gaaacattta ccggaagact gttttccatt catttgttct ggaagagaag
      181 tagaagacat taataatatt ctcaaaaaat ctggtttaag gataccagca gtagggttaa
      241 atggttctgt tggatttgat ggagaaaaaa agatatttga gtcttccttc gataaaaatg
      301 atgtaaaaaa aattgattct gtcctatcga aatttccaac caaaatatat acaaatctcg
      361 gaagttatga atctcagaat tataaaaata aaatgaaaaa aatttttcac gaggtaggga
      421 gtgaattttc tatcaaagag ctaaactatg agcttgagta tgaaaaatct atttgttcaa
      481 tcccttataa taatattgag gaaattatag aaaaggatgc tatcagcatt tacaaatttt
      541 ttatttttat tcctaatcgt caattgaaag ctaatatatt taaccatttg aaaaagatgc
      601 taaatgtgtc tgttactgaa tcggcacctg taaatattga aatcgtacct caaaatgtat
      661 ctaaaggtgt ggtgtttgat cgtttggaat caatctacaa tttaaagaaa cctttgagaa
      721 tcgcgatcgg agatagttta aatgacttat ctatgtttga aagtgcagat attagttttg
      781 caatgggaaa tagtcatcaa gaaatcatga aaaaagctga ttatgtcact gtttcaaatg
      841 ataaagacgg agtatctcaa gcatttgaaa gaattcttac catataaact aattaaggta
      901 gtatagaaca tagaattcta aatacggaaa aatggagcat aaattccagt acaaatagta
      961 cttcttcagt gtcaaacgaa gcgaatataa catagatatt taggattgtg acaaaaatca
     1021 tgtcacaatc tttttttcga ataaacttat tttttgaagt cggccattta tcgtaatttc
     1081 tttgcataat acgaacaaat tttcggttat tgatgaaaaa aaacattgaa aatggtatga
     1141 taaaagaaaa aaaggaggaa atttttaatg gtgaaattta tccatgcggc agatttacat
     1201 atggatcggt catttgaagg actgacaact cttgataaaa ctgtacagga aaaactattg
     1261 aagacgaatc tgaccgtttt atcaaatatc attgaacagg caataataaa taacgttgat
     1321 tttgttttac ttgcaggaga taattttcat caaaatcgtc cttcattaaa aatacagaaa
     1381 catttttcag aacagatgaa acggttagaa aagaaccata tacctgtatt tataatattc
     1441 ggaaatcatg atttctatca aaaagagcgg tattggttct cctttcctaa aaatgtccat
     1501 ttatttacat ccgaaacggt agagacaaaa aaaataacga taaaaagtgg agaaactgtc
     1561 tctctcagtg gatttagtta tcgtcaaccg tggattcaaa aagacaaagt gatggaattt
     1621 ccttctagag aactcacaga ttaccatatt gggttgtacc acggggaacc tggcgtctct
     1681 caaaaaggaa actatgctcc tttccaaccc tcaaagatgc aagaaaaggg atacgattat
     1741 tgggctttag ggcatatcca tgtgccaacg gtcttaaatg aaaagcaaac aattgtttat
     1801 ccaggtgcgc ctcaaggtca tactaaaaag gaacaagctt ctacatctat tctactagtt
     1861 gaattaagta aaggaagttg ccaaatacat ccgattaaag tagcagaggt ctattggaag
     1921 acaaaggaaa tatcattgag gaaagcaaga acgacaaaag aggtcattgt tcacattcgt
     1981 cagcagcttt caaaagtaga ggatggattt agtttaattg agataaaatt aaaagattat
     2041 gaccatctca atacagatgt tttggaacgg atacaatcag gagagctatt agactatttg
     2101 ttagaggaat tctcagatag gataaatgat ttctttattt ggcgtatttc tctcatagag
     2161 aatacttttt atacgaagac cccacttgct gcttctgcta aattgatgga acagttattt
     2221 caatactatc agacacctca agatttccaa caaattttaa atgaagtata tagtcatcaa
     2281 gaagcggcta gaatattgag cgaactgccg gaatatcaag aagaaacact tgaaaaagcc
     2341 aagcagctgc ttaatcaaga ttttttattt gaggaggatc aggaatgaag attttaagga
     2401 tcgagattgc tgcttttgga aaatggcgtc aaaaaagttt tgacttttat tcaggcaatc
     2461 agctgatcta tgggggaaac gaagcaggaa aatcaacgat ttatcaattt attcaagcaa
     2521 tattatttgg tttcccttca aaaggccgta aaaaaaagga ttatacacca aaagacggct
     2581 cagcctatgg cggcaaaata tggcttaaac atcctgtata tggtgaattt gctgtcgagc
     2641 gctacaagca gcaaaatcgc ggaaaatcga aggtgtggct gggggatcaa gtgggaagtg
     2701 atgagttact agaaaaactg atcgcacctt tgacgaaaga gttattccag caggtattta
     2761 ctttccagca agagcagttg atggaactag atcatttgag agaaaaagag ctacatgatg
     2821 cgctgatttc tttaggcatc acgggcagtt ctcaagtatt ccaaaagcgg caagaatatt
     2881 atcagaaagc acaacaatta ttcaagaaaa aaggccaaaa attactaatc aatcaaaaat
     2941 taaatgaatg gaaagagctg caagaaaaaa ttcatcaaaa acaagaacag gaagcttatt
     3001 ttggcaagtt gatccaacaa gaacaggaat acgagtcgca acagcagcag ttattggcac
     3061 aattaaaaga ggcgaacaaa caattttcat tagccagaca gcaagaactt aatttttctt
     3121 tatatgaaga atggcaatct ctcaaaaaaa acagcactac aggtcttcct gatgagcaac
     3181 ttcttttaga tctggagtct ttctccaaac ggtatcagca aacaaatgag cgtttggaag
     3241 aattagaagc aacactggaa aagcatagcg ggatggatca gcagtcagcc agatattatt
     3301 tttatttaga gcaggagcag gaactaaaag atctccagca acttcaattt acagcggaac
     3361 aattaaacga agaatggcaa agattacaaa ttgagactag agaaacaaat tcagaattga
     3421 agattttgga acaaaggtgg cattggcaga aaaatccgtt accggaagaa tttttatatg
     3481 aagaagaatg gaagcatctt ctggctgaag aaagaaggat tgctgatcta gaaagccaga
     3541 tttctgtgcg tctccaaata gctaaagaac agcaagagat gattgaagca gaagtgaatg
     3601 acttggaaga gacctatcca gcattattac aaaatcagtc agagatagca ccagtctctt
     3661 catctaaacc gtatatgctg atggctggcg gagcaataat cattgcaggt atatttgtac
     3721 caatgccctt gaaagtgatc cttttactaa ttggtgcagg catgattggc tatttcttct
     3781 acaaaaggca accaaaaatt aaaactaaaa atgatttctc tgagataaaa ggaatgtggc
     3841 aagaaaaact aggacaactt gatctagcct tagctcaatt agctgaagca gaagaaaaac
     3901 aaaagagaac tggagaagaa aaacagattc ttcagcaaga agtatatcgt tttgcggatc
     3961 atcggcactt aggaaagatg gatacgctca aactcatgaa agaatatgga caggaagtca
     4021 tagattatca gaaattatat ggcgtatttg agcggaaaca taaaagagaa caggaaatac
     4081 aagaaaaact acgacaaatc gaccaaaaat ttgagtttgt taaagaatgg ttaccgatac
     4141 aagataagac aatttcagaa aaaatggtat tgctttcccg ttttatacaa gagatggaac
     4201 agatgaaatt tgccagaagc tatcagcaaa atactttatt gaagcaagaa atcaatcaat
     4261 tgaaaaagaa acaaaaagta tggatacaag aatacagcga acaattgact caagcaggct
     4321 tatcttatcc ttcggaagta cctgtctttt tacaaaaagc caaacaagcc gaacagcaga
     4381 tgaaaagata caatgaatta tcggaaatgc ttactccact atttcctgaa gagctgacaa
     4441 aagagaaatt atctgtaaga ctgcgccaac tgaaagaaaa gcaatcccaa ctccagcaag
     4501 aagaaaaaga agcaagtgaa aatcggcaac gactccaact tcaagtagaa gagttgcaaa
     4561 gagacggcac attggacgaa ctttatcaac aagctagccg gcaaaaagca aaattacagg
     4621 aattactgga ggaatgggga gcacttgaaa tcgccgggac attcttaggt gatctttcaa
     4681 cagagatgag tgaaagacaa ttaccccagt tgttaagagc agcaggtgat tatttaggta
     4741 ttttaacaaa aggaaattat cagaagatat tactgaaaaa tgatacactg actttaacag
     4801 acggatatac agaatttccc atctatgagt tatctacagg tacgaaagat cagctgatta
     4861 tggcgatgag atttgcttac ctctcattag aagctaaccg ttcgatatgc ccgatcatca
     4921 ttgatgatgg ctggcttcat tatgatcatc agcgaaagta tcaattagct gaacttttag
     4981 ctaaatttgg agaaaagtat caaataattt gtttttcttc tgataaagaa atggtaagct
     5041 attaccatga gatgaaccaa atcgtaaaac aattagaagg agcaaataat gaaaaaaatt
     5101 cgtgatttag taatcgatga gcagtttgaa caatttgtcc tgatcaaaag tgcagatgta
     5161 agaatcgcta aaaacggaaa aaaatttatt gcttttactt tccaagatac ttcaggtact
     5221 atcgatggta aatactggga tgcttcggag gaggaaatca ataaatttac agccggcaca
     5281 gtcgtttttc taaacggtaa aagagaggtt tatcaaggaa accctcaagt aaagattctt
     5341 catatgcgct taacaagggt agatgagcca ggagagccta gtttatacat ggaaagagcc
     5401 cctttgaaga aagaagaaat ggaagaagaa atcaatcaat tgttgtttga gatcaccaat
     5461 gcgcattgga atcgtattgt tcgctatcta cttaataaat atcaaaaaca attttttgag
     5521 tttccggcag caaaaagaaa ccatcatgca tttgccagtg gattagctta tcatactttg
     5581 acaatgcttc acttagggaa atcaatcgcc gatgaatatc ctgagctgaa tcgagcattg
     5641 ttgtatgctg gtatcatttt gcatgattta ggaaaggtga ttgaactttc cggtcctatg
     5701 tcgacagaat atacgcttgc aggaaatttg atcggtcatc tggtattgat tgacgaagaa
     5761 ataactaaag cctgtcttgc gttgaaaatc agcgaagctg acgaagatgt tctcgtatta
     5821 cgtcatatgg ttttagcaca ccatggctta ttagaatatg gctcacctgt acgtccaaga
     5881 ataatggaag cagaaatatt acatcaaatc gacaatatcg atgcttcgat gcagatgatg
     5941 ttgacttcga ttcgtcaaac agaaccagga aaatacaccg atcggatttt cggtatggac
     6001 aaccgaagtt tctatgtacc aaaagattta tagcaaaaat ttattttttt cattcaaaac
     6061 aaaa
//
LOCUS       DAZKMB010000135         6052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000135 DAZKMB010000000
VERSION     DAZKMB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.138"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            205..1836
                     /locus_tag="ACG8V3_002531"
     CDS             205..1836
                     /locus_tag="ACG8V3_002531"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0301003.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1839..2918
                     /gene="uxuA"
                     /locus_tag="ACG8V3_002532"
     CDS             1839..2918
                     /gene="uxuA"
                     /locus_tag="ACG8V3_002532"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0301004.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2992..4419
                     /gene="uxaC"
                     /locus_tag="ACG8V3_002533"
     CDS             2992..4419
                     /gene="uxaC"
                     /locus_tag="ACG8V3_002533"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0301005.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4503..5150
                     /locus_tag="ACG8V3_002534"
     CDS             4503..5150
                     /locus_tag="ACG8V3_002534"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0301006.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5270..>6052
                     /locus_tag="ACG8V3_002535"
     CDS             5270..>6052
                     /locus_tag="ACG8V3_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0301007.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMN"
BASE COUNT         2083 a          818 c         1253 g         1898 t
ORIGIN      
        1 gaatacatga aaaaaagaaa aaataagttt cgataaaatc agtccaaaaa gaaggagtat
       61 tttatttttc tgaataaaaa atacaatcat tctgatatgg tagaaaaaag caagtttatt
      121 ccctttttat attgacaacg gttacaatgg gttttaaaat ataaaatgta tcaaaactaa
      181 catatcagtt tgaggaggtt aattatggtt tcgattaaaa attggcaaaa ttctatcgaa
      241 gaattcaatg aaatgcagat tctactgccg tgttttgatg tcaaagaagt taagcaagaa
      301 ggccagcaac atccagtatg gcttcatttt ggtggaggaa atctttatcg aggatttcat
      361 gcagaaattg ctcaatgctt agcagatcaa aatgaattag aaacaggagt agttgtgtgt
      421 gaaacatttg atgaagaagt agtggaaaaa gcttatcatg actataacaa tgacattttg
      481 gaaattgtta tgagtggaga tggtgaactc aaaaaaagac ttttggcagt aacggctgat
      541 agtttatatt gcaatccgaa gaatatagcg gactatcaaa gagtggttaa atattttgaa
      601 gatcctaaat tacagtttac aacgtttact attactgaaa aaggttacag tttaaaagat
      661 tctaaagggc aattcttttc ggtagtcaaa gctgatatca aatctggacc tagcaatcct
      721 caacatacga tgagtattgt aacatccttg ttatatagca gatttaatgc tggagagttg
      781 ccgattgcga tggtttctac tgataatttc tcacagaatg gtcaacgttt tcaagatagt
      841 atttttacga ttgcagaagc ttggcaaaaa gaaggatatg tttctgaaaa atttattgct
      901 tatttaaagg ataaagaaaa agtgactttt ccttggagta tgattgatcg aattacgcca
      961 aatccttcag agaatgtaaa ggaagatcta ttgatgatgg ggctcgagga tttagatatc
     1021 attcacacaa aaaaacatac caatattgct gctttcacta atactgaggt agttcattat
     1081 ttagttattg aagatagttt tccaaatggc cggcctaaat tggaaaaggc tggtgtgatt
     1141 atgacagata gagagacggt tgataaagca gacatcatga aggttacgac aagcttgaat
     1201 ccacttcata ctgctttggc agtaacaggt tgtctgctag gatatcaatc aatttcagca
     1261 gagatgcagg atgatgattt ggttggttta atcaaaggaa taggttattt agaaggttta
     1321 ccagtagttg aaaatccagg cattattgat ccaaaacaat ttattgatga agtaattaat
     1381 aaacgtttgc ctaatcccta tattccagat acaccacaac gaattgcaac agatacttct
     1441 caaaaagtag cgattcgcta tggtgaaaca attaaaaagt atgtcgaaaa agacggaaat
     1501 gcagaaaatc taacatttat tcctatcgct attgctgctt ggttacgtta tttactggca
     1561 gtcgatgatc aaggggaaaa ttttgtgcca agtcctgatc cgttattggt agagttgcag
     1621 gataaattat cagtattgaa attaggtgaa caagatcctt caaagattca ttgtgcggta
     1681 caggaaattt tagaaaataa agaaatcttt ggtttagatt tatatcaagt tggggtagga
     1741 aagaaaactg aaaaaatctt ttgcgaaatg ctagtaggtc ctcaagcagt tcgtaaaaca
     1801 atcaataaat atataacaga agttggagga aagtaactat ggaaatgact tttagatggt
     1861 atggggcaaa tgaagaaaaa atctcactac aagaaattcg tcagatccca gcaatgaaag
     1921 gaattgttgg aacattaatg gatataccag ccggagaggt ttggccgaga gaacgaatta
     1981 aatctttaaa agatgaagtc gaagccaatg gacttacctt gaaagttatt gagagtgtta
     2041 atgttcatga tgatatcaaa attggtttac caacacgaga tcaatacatt gagaattata
     2101 aacagacgat tcgcaatttg gcagaatttg ggattgaggt tatctgttat aattttatgc
     2161 cagtatttga ttgggtaaaa tcggatttgg attatcgttt agatgatggg tcttctacgt
     2221 tagcctatat ttcaaaaaat atccctactg atccggagga aattattaga gaagtagaaa
     2281 attctactaa taattttgaa ttgccgggtt gggaacctca tcggttatca caaattaagc
     2341 aattattaga agcctataaa gaagttgatg aagagaaatt acgagagaac tttgcatatt
     2401 ttctcaaagc aattatcccg acatgtgaag aagttgggat taaaatggca gttcacccag
     2461 atgatccacc atattccatt ttcggattac ctcgtattgt taaaaatcgt gacgatttag
     2521 attggttgtg taatgtagtg gattctcctt ctaatggtat tacattatgt actggaagta
     2581 ttgcagaacg accagataat gatgtctatg aaattctatc tgaattttgt caacgggatc
     2641 ggattccttt tgcacatgtt cgtaatatca aatatttaag tgacaatgga ctcgattttt
     2701 acgaagcgcc gcataaatca gaatatggtt ctctagatat gtataagata ttaaaaacaa
     2761 tgtacgataa tggatttaat ggctatattc gtccagatca tggtcggatg atttggggag
     2821 aaaccggtag acctggttat ggattatatg accgagctct aggtgcagtt tatttaaatg
     2881 gtatttggga agcgcttgaa aagggggcga gtaaataatt atataaggga gtttatttag
     2941 aggaaaaatt ttctcttcta aaaactaacg gaaggaaagg agtgtttaaa tatgttttta
     3001 gataagaact ttttattaga gaatgaaata gctaaaagac tttttcataa atttgcaaaa
     3061 caccaaccaa ttatcgacta tcattgtcat ttagacccag catatttgct agaaaataag
     3121 caatacaaga atttgactca ggtatggtta aatgataaag gagctggaga tcattataaa
     3181 tggcgattga tgcgagctaa tggtatagaa gaagagttga tcagtggtcc aggtgatgat
     3241 tatgaaaaat ttttagcttt tgtaaaaaca gtggagaagg cgccaggtaa tcctattttt
     3301 gaatggtctc atttggaatt acaacgttac tttgatattg atttgacaat ttctgagaag
     3361 aatgcaccag aaatttggcg acgtgctaac gaaaaaattc aaacggattc ctatcgcccg
     3421 aaagaattgt tggagatgat ggatgtgaaa gccatctgta caacggatga tcctacagat
     3481 gacttgtggt atcatactga gctttctaaa caggataatt ctttcaaggt attacctact
     3541 ttccgtccag ataaggtctg gagtatcaaa tcatcaggct tttcgaacta tattttaaaa
     3601 cttagtcaaa cagcggctat accaatcaaa gactttaatg ggttgaagca agcattgagc
     3661 caacgagtgg atcattttca tcaaattggc tgtcgattag cagatcacgg tttgaatact
     3721 tatttttatg aaatagcaga tgaaaaaata ctcgacaatc tgttaataaa agctttagca
     3781 ggaaaaacgg atttccattt atcagaaatt gcacaatttg ttacaggtat tcaattacat
     3841 ttgatgtctg agtatacaag atatggttgg acgatgcaaa tgcatttaaa tgctttaaga
     3901 aataattcaa gtatattaaa ggaacaaatt ggagtagatg tcggtggtga ttcgatgggg
     3961 gatcaagcta gtttgacaca aaatgttgta gcattgttcg ataccgctga aaagagtggg
     4021 tgcttaccaa aaacaatttt atattcatta aatccaactg attggcttcc actagcaaca
     4081 gcaatgcaaa gcttccaagg gggtatgatt caaaaaatcc aattgggatg tgcttggtgg
     4141 ttcaatgata cagctgaagg aatgcgtcaa cagttgacta cgatggcaca acaaagtttg
     4201 ttgtcgaatt ttacaggtat gttgactgat tctcgaagtt ttctaagcta tcctcgccat
     4261 gagtattttc gtcgagtatt atgtcaatta ctaagcgagt gggttaaagc aggccgaatt
     4321 ccagatgatg aaatgtatct tagtcagata gtggagaata tttgttatcg taatgcgtat
     4381 aattatttta atttttttga aatcggaagt agaaaataat acttgaaata aaagcttaaa
     4441 tttattcttt gtaagttagt acagctagac aaagtatgta tccataaagg aaggagatag
     4501 atatgaaaaa agtagaaatt ttaagtaagt tagaagcggc tggagtcatt gcggttgtac
     4561 gtgggaaaaa taaggatgag gccattaaag caataaattc aattattgcg ggtggaatta
     4621 aagggattga actaactttt acagttccta atgctgaaga aataatcaaa gaaattgcta
     4681 aaaaatatgc tgatcaaaaa gatgtaataa tcggagccgg tacggtttta gatgcgttta
     4741 ctgctcgtat cgcaattatg gctggggcag aatatatcgt tagtccatct tttgatcgac
     4801 aaacagctga gatttgtaat ttatatcaaa ttccttattt accaggttgt atgacaatta
     4861 ctgagataaa ggaggcactt actgctggcg tagatatcat taaactattc ccaggttctg
     4921 cttttggtcc atccattgta aaagcctatc aagctccatt accacacgta aatataatgc
     4981 ctactggcgg agtaagtata gagaacttag aagattggtt tgaagtagga gttgttgcag
     5041 ttggtgtagg tgggaattta ttggcttctg ttgaaaaagg tgattttgaa gaagtaagtc
     5101 gagtagctag taaatatata gataagtttt atgagataaa gaattattag gagaaaaaac
     5161 aaataatact ctgaaatgta aaaaataaat actgactctt tacgcttcag agtatagtac
     5221 gtactttcat cagaatgaaa acgcttaaca acagaaggga gagaattgta tggaaagaaa
     5281 atttggtata agagacaaaa taggatatat gtttggagat tttggaaacg attttacttt
     5341 catttttgct agttcttttt taatgatctt ttatacaaaa gtattgggga taagcggagc
     5401 tgccgttgga acattatttt tagtagcaag atttatcgat gcagttactg atgtaactat
     5461 ggggcgtatt gttgatagat cgaaagcaac taagcagggt aagtttaaac cgtggatttt
     5521 aagaatgagt ggtccagtcg ctttagcaag ttttttaatg tatcaaactt ctatgatttc
     5581 agcttctatg agcgttaagg tagtatacat gtatgttacg tatatattat gggggagtat
     5641 tttttacaca tctataaata ttccttatgg atcaatggca tcagctatta cttctattac
     5701 agaagaaaga acaatgctat cgacattccg aaatgttgga gctactttag caagtttggt
     5761 cattggtgta ttaacaccat tatttattta tacaagagat gattcaggcg ctcaaatagt
     5821 tcgtggtgga tcaactttta caattgttgc gggaatattt tcgatatgtg cacttatttg
     5881 ttatttaata tgttataaac taactactga acgagtaaga attgaccaat ctcaatacaa
     5941 agtttcattg ttaaaatctt taaaagggtt agttaaaaat agggcattac tttctataat
     6001 tggtgcggct attttcttac tccttgctca attattaatt atgagtatga at
//
LOCUS       DAZKMB010000136         6014 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000136 DAZKMB010000000
VERSION     DAZKMB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.139"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..1556)
                     /locus_tag="ACG8V3_002536"
     CDS             complement(105..1556)
                     /locus_tag="ACG8V3_002536"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0301008.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(1877..2641)
                     /locus_tag="ACG8V3_002537"
     CDS             complement(1877..2641)
                     /locus_tag="ACG8V3_002537"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0301009.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2669..3367)
                     /locus_tag="ACG8V3_002538"
     CDS             complement(2669..3367)
                     /locus_tag="ACG8V3_002538"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0301010.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3379..4158)
                     /locus_tag="ACG8V3_002539"
     CDS             complement(3379..4158)
                     /locus_tag="ACG8V3_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0301011.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4174..5118)
                     /locus_tag="ACG8V3_002540"
     CDS             complement(4174..5118)
                     /locus_tag="ACG8V3_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0301012.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5164..5943)
                     /locus_tag="ACG8V3_002541"
     CDS             complement(5164..5943)
                     /locus_tag="ACG8V3_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301013.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1631 a         1260 c         1001 g         2122 t
ORIGIN      
        1 tttatctgta atgcgtcatc aaaatctaga ttccaaatct ttatcaattt ttatccactc
       61 attctaccac tttagtaata ttttctattt ataacacttt cgtcctacat tgatcccccc
      121 ctctgaagta cagtttttac tgtcttaaat aaaattttaa aatctaaccc catactccaa
      181 ttttttatat attccgtgtc aagttttgct acttcttcaa aatcagtaat atcacttcta
      241 ccacttacct gccacattcc agtaattcct ggcttaatag caattcttgc acgatgatga
      301 taatcatatt cttcatactc tgatttaagg catggtcgaa atcctataag gctcatcttt
      361 cctgttagaa cgttgaaaaa ttgagggaat tcatctaaag acgtggatct aataaattga
      421 ccgattcctg tcttttttgt tccatctgga agtattttat ttccgataat tcttggatca
      481 aactccatct taaacataag attattgctc atcttatttt ctttcattaa ttctactttt
      541 ctttcttctg catccatata catacttcta aacttataca tcttgaacgg tttaccattc
      601 tgacctatac gttcttgtgc aaagaaaatt gggcctggtg aattaatata aataagtgga
      661 gcaataaaga taaaaataat tcccgttgca atacatccaa caatacctgc aagaatatca
      721 attgttcgtt ttataaatgc ctgttgagtt gtcataacat tgatacttgt tgtaagtact
      781 gtatattctc ctatacgacc aatagattgt tttcccaaag cactatctga tacttttgca
      841 agattcaaat gtacagtaag gcccatttca gagcatcgtt caataaattc tttcatacac
      901 gaagtacttt tattcaaaat aataaagact tcatctaccc atccctgaca taagtaatcc
      961 gctgcatttt tggaatcaga aacaactgtc actccctcaa catcttcacc aatcatgctt
     1021 ttatctatga taatgacacc atttatttta tacattttat agttatgctc tttcacatta
     1081 cgaataacta cttcagcaat actactagat gtaactataa taagtgattt gttcccctca
     1141 tttttcattt tatgtttcaa gtaatgtttc cataagaggc gagttatata tgtaattaca
     1201 acgtataaac ctcccatcaa gaaaagaacg actctagaat aatcatttcc accctgaaca
     1261 ctaatgagat acaaacttcc taacagcacc aacataatcg cttgctttac agaagaagca
     1321 aattctatat agtaccctct ttttaataca cctctatatg cttcaaacat gaaaataact
     1381 aaaagatctg ccagttcaat gaagactgcc atgttttgat acattggaat cacataagga
     1441 ttccaatcta cctggcgtaa gacatacgat agataaaaag caagctggat acacaataaa
     1501 tctaaaataa taaaatcaat atgtttaacc catccccttg aacttttctt gtacattcct
     1561 ttttactccc ctctttttaa taaaataaat tgtattactt gttttctctc ctaaatatct
     1621 ttctgaagga ttattgactc gacaaattgg cttgtggata ttcttatata tagaacatcc
     1681 gtaagccaac ttaaaactcc ttaacaaaat ttttttactc atattgtaga tcaaaacaag
     1741 tatccaagaa atcatatttt atttcttttt tctgcttact caacaacagc taaaagaaaa
     1801 atcatcataa acaaattcaa ctaagccaac gatccccttg tgaaatcaag ttttttcttg
     1861 atttacgaat aaagagctaa aataaaccaa acttcttctt ttttacctct tcatagactg
     1921 gataactgat ttcatccccg ttgatcagat cctttgcgac ttgtttcatc tgtactactt
     1981 tttccttacc aaagtccttt gcaatctgct catagcactc ttttaagtaa aaggtgcgct
     2041 tagagacccc atgggcatca gaagcagcca tctgcacgag attatgtttg accatcgttt
     2101 tggctgtttt ctggatgtct ttgccaaata ccccaacata acttggcgca gtgagttggg
     2161 ccaaacatcc catatctagg aacggaagca aatgatttgg gtcttcgcga aaatgcacgt
     2221 tgcgttctgg atggacgatt actggtgtct ttcctagttc taacaactca aaaaaaagcc
     2281 gttctgcata taacggcact tccagtgtcg gaaattcgat cagaagatat gtatcatcta
     2341 aatctgtaaa taatatttca tctcggtgga tgtcttcgat gagcgttccg gtaatacgta
     2401 cttcttgtcc ttccaataac gtcaaaggca actgtctttt ctctaattcg gcttgtaacg
     2461 aagcgactag agaaatgact tgtgattttt cattgctgta ttttccatta ttgtggtgtg
     2521 gtgtgcataa aatatgcgtg atgccttgct gaatcgcttt ttcagccatt gcaatacttg
     2581 cttctaagtt ttctgccccg tcatcgattc ctggtaggat atggcaatgt agatcgatca
     2641 tttctcttgt ctcctctctt cttattcctt aatttccgta gtaataacca gcatctttgc
     2701 tatgttctgc tccgttgtac acaacaccta agactcgtgc ttgtaccatc tccagcagtt
     2761 cttttgcttt agtcaaggat tctttacgtg acatattttc acgtacgacc aataaagtgc
     2821 catccgtttt taaagacatg atctgtgcat ctgttacggc tactactggt ggcatatcga
     2881 atatcaccac gtcaaataac tgtcttgctt cggataggat ctgatccatt cttggcgagc
     2941 ttaacaattc tgatgggttg ggtggcttag gtccactagg caaaatcgac aagttctcga
     3001 ctggcgttcg ttggatcaca tctgctacac ttcctgatga actaagtgct gtacttaatc
     3061 cactggcatt gttcagctgg aatgtcttat agacgacggg tttacgtaaa tctgcatcga
     3121 ctagtagaac tcgttgacct gatttagcaa aaacgactgc aatgtttgct gcagtggtgg
     3181 atttcccttc tccaggacct gatgaagtca cgacaatcgt tttgatttgc tgaccagctg
     3241 cggaggcaaa ctggatattc gtccggatcg tacgatattg ttcggcgatt ggtgaagatg
     3301 ggtttgttaa tgtgatcaaa ctgactgcat gagtttgtgc tgctttttgt ttctgtgttc
     3361 gtgccatttt tcttcctcct agactcttga gcggcttctg cgtgatgggg ctgttttatc
     3421 gtttgtagct tttggtcgtg caggagctgt tcctttttta cggataccag cgttcaattc
     3481 tttcgctgac atttctggaa ctgttcctaa gattgggaat cctaatgtat ctgtcacata
     3541 tttttcatct ttcactgtcc gatcgagtaa ttccaataag aatgccagac ctacacctac
     3601 catcattcct aacattaagc cagtcattaa attcaattta ttatttggcg aaacaggtgt
     3661 cgtattggca actgcacctg aaatgatcga gattttatca acattcatca catcttttgc
     3721 gttctcttgg aaaacttgtg aagttgtgtt agcgatctgt tgagcagcca ctgcattcgt
     3781 acttattgcc tgaatcgaga acatttgtga attttctgcc tgattaactg agatggcatc
     3841 tttgatttgt tcagcagtca ttttcacatt gtcttctgtt tctaaacgat ctttgacttc
     3901 attgagcact aaatcactga cgatcatatc tttatacgta ttgatcattt gcaggttcgt
     3961 attgacatca ttgacatttg cagtgtctga ttgtggcagt gtcacgatca attgtgcttg
     4021 tgaactgtat tttggtgtca agatgaaaaa tgttacgatt cctgagatgg ccaagccagc
     4081 aaacattgag atgatgatta ttaccaagtg tttgcgtaat gtttcaaaaa tttctttcaa
     4141 gctgattgtc tcttccattt tttctccact ttcctattta ttctctagat tcatttgatc
     4201 ttttaattct tttttcacac gatcaagttc ttgatcactg actttttgat aggagatatt
     4261 atcttgcatg aagccttctc cttgcagctg atcttgctta atcgtaccaa atgcctcccg
     4321 ataatctaaa gcaatttttt tcatttcatc aaagttcatg tctgttttca tattcgactc
     4381 tagagcattc aaaacggatt gatagttgct gacaccgttt agactcaata cttttttcgc
     4441 tacgccttct acgatctttc tttggcgccc ttgtctaccg taatcaccat ttgggtcttc
     4501 ataacgcata cgagaataag ataatgcctg atccccgttc aatgtaattt taccgattgg
     4561 aaagtcatag ccatcttgtg agaaagtgag gtcattattg acttcgatac caccaactgc
     4621 atcaacgagt tcctttagtc ctttcatatt gatagagaca tagtgatcga ttgggatgtc
     4681 tacatatttt tgtacggtat ccatcgccat ggcagctccg ccgaatgcgt aagcgtggtt
     4741 gatcttgtct tttgtgtcac gacctacgat gtcgacatac gtatctcgag gaatgctgac
     4801 gatcgttgtc tgtttgtctt caggactgac tgtggcaacc atcatcgtat ctgatcttcc
     4861 ttgatcgacc cgacctaaat ctccagtatc aatccccatc aatagaacag agaaagattc
     4921 ttgtttgtcc agatcaactg gtttcgttcg ttctccttgg gttcgttcca ctgattcgta
     4981 agttttctca atagaatgtg agagatccag atataatttt gctcctactc ctacaattgc
     5041 taatactaat acgataagaa ctgctaaaat aatcaaaaat atttttttcc ctcttgattt
     5101 tcttcttcgt cttgccatag tttcctccgt ttgctaacct tgcttgaatc aacgttttaa
     5161 ctgtcaatag cttatattgc tatagcccat ttgctgagca acagtttgtg cttttgttgc
     5221 gtatgcggct cttatttttt cattccggat ctgctctatt tgactgagcg ccgtcaaata
     5281 ttgatcgtaa gtagcggtag ctgttacttg accgtctttc agcatcgtat cgaatagctg
     5341 atctagttgc gtgacttgtg caatttgggt atcagcgaat cctagatatt gcataacgac
     5401 tgttttccaa gcactatcct taaaaaagct catattttca cgtacatggg cgacatctgc
     5461 acttgccaac ttttctttga tgatgacatc atccacagct tgctgccagt ttgatacgtt
     5521 tttagtaaat aatttattga cagccgtctg gatcttcagt tttgattctg catcctctaa
     5581 ctgcgttaag actgctttct tttcttcttg gatcgcagca gcttctttag gtaattctga
     5641 ttccttgatg tcataatcat ctgccgaaag tttgatagaa gatagtttat gtgacaaatt
     5701 tgaaactgtt tcttctgtta tttcaggatt taggaaaatt ttttcttttg tactgtacag
     5761 cttatctact tcttttgata aagaggtcac ggtgtctttt tgattggtga tggcagattc
     5821 tgcgtaattg atccgttgat gatattgccg ctgattattg agatggtatc caaacaagca
     5881 aatcacaata gcgcctgtgc ctaccgccac agtagccaat aacttgacga acggcttttc
     5941 catagattca ctcctaaata ttgatgatag ataagtaaag atgtgattca ttcacatcag
     6001 aatgagcgct tttt
//
LOCUS       DAZKMB010000137         5836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000137 DAZKMB010000000
VERSION     DAZKMB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.140"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..384)
                     /locus_tag="ACG8V3_002542"
     CDS             complement(<1..384)
                     /locus_tag="ACG8V3_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301014.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="ACG8V3_002543"
     CDS             complement(569..967)
                     /locus_tag="ACG8V3_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0301015.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="ACG8V3_002544"
     CDS             complement(964..1737)
                     /locus_tag="ACG8V3_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0301016.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="ACG8V3_002545"
     CDS             complement(1734..2417)
                     /locus_tag="ACG8V3_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0301017.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="ACG8V3_002546"
     CDS             complement(2584..3471)
                     /locus_tag="ACG8V3_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0301018.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="ACG8V3_002547"
     CDS             complement(3468..4337)
                     /locus_tag="ACG8V3_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0301019.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="ACG8V3_002548"
     CDS             complement(4474..5760)
                     /locus_tag="ACG8V3_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0301020.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1795 a         1297 c          963 g         1781 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttc
//
LOCUS       DAZKMB010000138         5695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000138 DAZKMB010000000
VERSION     DAZKMB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.141"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2144
                     /locus_tag="ACG8V3_002549"
     CDS             <1..2144
                     /locus_tag="ACG8V3_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFQ0301021.1"
                     /translation="FPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTDLEGT
                     SAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKTKGNV
                     LWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWKQKGL
                     VFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVLFEYS
                     RSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDALASAT
                     LSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEMAAGA
                     AVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIKHLIQ
                     LISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQVANNR
                     LNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPSHSKT
                     AIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQESFFFS
                     GTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGGQRQR
                     LALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAKACDQ
                     ILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2113..3528
                     /locus_tag="ACG8V3_002550"
     CDS             2113..3528
                     /locus_tag="ACG8V3_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0301022.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3561..4595
                     /gene="sppA"
                     /locus_tag="ACG8V3_002551"
     CDS             3561..4595
                     /gene="sppA"
                     /locus_tag="ACG8V3_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0301023.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4597..5118
                     /locus_tag="ACG8V3_002552"
     CDS             4597..5118
                     /locus_tag="ACG8V3_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0301024.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5115..5501
                     /locus_tag="ACG8V3_002553"
     CDS             5115..5501
                     /locus_tag="ACG8V3_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0301025.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2043 a          776 c         1079 g         1797 t
ORIGIN      
        1 catttccttt tgtgcagcaa caagatgaaa aagattgtgg tgttgcttgc ctttcgatga
       61 ttttaaaata ttttaaaact gatatcccga ttcataaatt acgggaccta tctgggactg
      121 atttagaggg tacttcagca tttggattga aaaagacatt tgaaaaattg tcttttgatt
      181 gtatggctat tcaagcagat agtgagatct ggaaagaaag agatcttatc ttaccattaa
      241 ttgcacatat attggttgat aacagctata tgcactacgt tgtaatttat aaaacgaagg
      301 gaaacgtact atggattgca gatcccgcta atggaaaaaa tagaaaaacg attgaagcgt
      361 ttcaaaaaga atggacaggt attcttttat tacctatgcc tagagaaact tatattccta
      421 ttaaagaaaa agtagctgga ttaagttctt ttttgcctat aatttggaaa caaaaaggat
      481 tagtattcca catcgtttta gcatcgattt tcattacgtt ttttggcata ggaagctcat
      541 attattttca aggtattctt gattttttca tccctaatca agcacgttca actttaaata
      601 taatttccat tggcttgatc gctgtctatt tatttcgggt attgtttgaa tatagtcgta
      661 gttatcttct tattatttta ggacagcgaa tgagtatggc aattatgctt cagtatttca
      721 aacatgtgtt aactttacca atgaattttt ttgctacgag aaaatcaggg gaaattattt
      781 ctcgttttct tgatgccagt aagattattg atgctttagc aagtgctacg ttatcaatat
      841 ttttagatat tggtatggtg ttgcttgttg gggttacgtt agctattcag aatagtacgt
      901 tgtttttaat cactctatgc tctttgcctt tttatgtagt tgcaatattc gctttcgtga
      961 aaagctatga aaaatcgaac agggaagaaa tggcagccgg tgcagctgta aattctagta
     1021 tcatagaaag tcttaaagga atagaaacaa ttaaagcata tagcggtgaa gagaaagtat
     1081 ataaccgtat agaccatgaa tttgttaaat taatgaaaaa atcatttcgt acggttacgc
     1141 ttgataatat tcaacaagga attaaacatt tgattcaatt aattagcagt gcattaattt
     1201 tatggctagg ttcttatttt gttatggata atagaattag tctaggtcaa cttatcacat
     1261 acaatgcttt actagtattt ttgacagatc ctttacaaaa cattattaat ttacaagtga
     1321 aaatgcaaac ggctcaagtt gcaaataatc gtttaaatga aattttcgcg attgattcag
     1381 aaaaatcgaa ccagtctttt gaatatatgc tttctgaaca attatttcaa caagatatca
     1441 ctgtaaaaaa cgtttcattc tcgtatagca tgaaggcccc aacgttgaac gaaatttcat
     1501 gtacaattcc ttcgcacagt aaaacggcta tagttggagt tagtggttct ggaaaatcaa
     1561 ctcttgcaaa gttatttgtt cgtttttatg aagcatcaga aggatcgatc cattatggaa
     1621 acattaatca cttagacatt ccttttcaga tattacgcga tcgagtgaca tatttgcctc
     1681 aagagtcatt tttctttagt ggaacaatca ttgagaatct tatattcggt ttagatagcc
     1741 caccaagttt cgaacaaata atagaagttt gtgagatagt ccaactaaat gaatttatta
     1801 atcaacagcc attacgttac gagacattgt tggaagaagg tggaaataat ctttcaggag
     1861 ggcaacgaca acgattagcc ttagcacggg ctttattaaa aaatccggac atattaattt
     1921 tagatgaagc aactagtgga ttagatacct tgttagaaca tgcaattatg aattatctcc
     1981 tcaatttaaa agaaaaaacg attattttta ttgcgcatca tttaccaata gcaaaagcat
     2041 gtgatcaaat tttagttctc catgaagggc atttggtaga aaaaggaacg cacgatgaac
     2101 ttcgttttgc cgatggtatc tatcaaaaat tatgggaaat ctaggagagg gaaacaattt
     2161 atgcagaata ataattggtt cgaacattct agtatctata ctaaacaaca tcatagcttc
     2221 taccgttggg tgttataccc tgtaattctt tttttatgta ttattggatt gtttttaacc
     2281 tttgctaaaa aagaggttgt tattcggaca acggctcaat tgaaccccga aaaaatagag
     2341 aagttacaag tgccattaga agctaaaata atggaaaact tcctaaaaga aaatcaattt
     2401 gttcataagg acgatgtgtt ggttagattg gattgttctt taatagagaa tgaaaaggct
     2461 cagatagaac aagaaaacca acgtattact caacaaataa agatggctca gctatttatt
     2521 gaaagtataa gtaaaggaaa aaatttgttt tcaacggatg acagttttgg ctacagtaat
     2581 caattaaaga gcatgttgtc agaaaaagaa tcactccgct acgctttgaa gcaaagtgaa
     2641 ttaaatgatc aaaagcaatt agaagtatac gaaaagacaa aaagacaact agaaaaacaa
     2701 attgagagtt cagatagtaa attacaagaa tggcaacaag tacaggtagc ttggagtaat
     2761 aatcaatcat taaaagattt ttcaaaagaa atgatggcaa actatgagaa ttggcaagaa
     2821 caactaaata atgtttctga tgatcaaaaa aatcaagtga aactgacaat ttcagcaagc
     2881 ataaatgaac aaattgagca actaaaaaaa gaagtagaac agtatcagtc agaaaaagct
     2941 aaattagtta aaccaactac ttctgagaat gacagaatta gtcaaacgga aaaaggaaag
     3001 caagagctag aacaagcaat aacaactgta aaacaaacag ttacagagct aaatgaaaaa
     3061 caagagaaga accagtcagt tatcaaaagt ttggacgatc aactttcaaa aggaatacta
     3121 aaagctcctg tatctggaac ggttcattta aatgaagaaa caaagggaca agcagaacta
     3181 gccaaaggaa cagtgcttgc agaaatttat ccgatccacg aaaaatcaaa aatgaaattt
     3241 acagcacttt tacctgcgaa tgaaagtacc tatattaaag aagggatgaa ggttcatttt
     3301 aaattggatc aaaaaggtgg ggctccgatt acgatcgacg gaactttaga tgaaatttca
     3361 gaaaattcta ctgccactga gaaaggtgtt ttttatgcag tgaaaggaac gttaaaaaca
     3421 actcaatcgt ttccgtttcg ttatggatta actggagagt tgtctttaat tgtaggagaa
     3481 aaaacatatt ggcaacaaat tgtcgatgtg tttctaaata aaaaataatt atttttacta
     3541 agaatatagg aggagtttat atgaataaaa gaagatggat tgctgtggga gtagcgtgcg
     3601 gattattgtt actatccatt atcgtcgcgc taattccagg aaaagataaa gaggaagcat
     3661 caaatacaac cctaacagga ataaataaga ttttttacgg atctaacgaa atcacagaag
     3721 aaacgttaga agaaggcgca tcaaataaaa agattgtcaa actatccgtc aatggagtta
     3781 ttgcagatac aggtgaaagc aatcttttta gtagggaaca atataaccat cagaattttt
     3841 taacacagct aaagaaaatt caagaagaca aagcggttaa gggagtgtta ttagaagtaa
     3901 attcacctgg gggaggaata tatgaaagtg ctgagattgc taaagaaatg gcaaaaatta
     3961 aaaagctaga tattcctatc tatactgcat ttaaaaatac agcggcaagt ggagggtact
     4021 acatttctgc tggttcagat aaaatatttg ctacggaaga aacaacaact ggatcaattg
     4081 gtgtcattat ttctggttta aattattctg gcttattaga aaaacttggc gtaactgatg
     4141 cgacgtataa aagtggtgca ttaaaagata tgatgtcacc tcaacacaaa ccttcagagg
     4201 aagagaataa agttatacag gaatttgtga tgagtgctta tgatcgtttt gtcaatgttg
     4261 tagcaaaagg aagaaatatg gatactaatg cagtgaaaga acttgcggat ggaagaattt
     4321 atgatggtaa ccaagcagta gaaaatgggt tggtggatca aattggttac tctgaagatg
     4381 cattagattc cctaaaaaaa gagaagaaat taactgatgc gacggttata gaatataaaa
     4441 atgacacaac tggctttgct tcttcttggt taggtaataa gattgctgaa tggcaagggc
     4501 taaaggcaac tacatcagat cgtgtaattt cagtttttga aaaacttgga ttttctgata
     4561 ctccaaaacc aatgtattac tatggagggc tataaaatga ataagaagag tggcattgaa
     4621 actttgccta attatgtatt tgcgggattt tggttacgat tttttgcttt tcttattgat
     4681 ttaatttgta ttagcatcat cacccatgct actataggtc tcttttacaa aatcatggga
     4741 ctggaatctg acaaatccat tattactttt tatggtatag cgtcattagc tatttattta
     4801 acctattttg tacttttgac taagctgaat tcaggacaaa caattgggaa aatgattttt
     4861 gggataagag tagtgagctt tgaggaaaaa gagctaagtt ggcaaactat ctttattcgt
     4921 gagtgtatat gtaggtttat attaaaaaat tttgtatttc aaattgggta tttggtggct
     4981 gctttcacgc ccaagaaaca acatattggg gattattttt ctaacacgtc agtggttgtt
     5041 attcgttcat tagaagctat taactatggt caaaaaattt ctaattctaa aacacttaat
     5101 ctggagagaa ttaaatgaat aagagaaaaa cagagtttat tttgatttta attagtggaa
     5161 taacagggat tttatgtagt cttttagcat tgatgggtgg tatgtttcta tcttctattg
     5221 attcaactac tttaaatact acagaactaa ccagtaagga tatagatatg ttagtcaatc
     5281 taagcgggaa tgttgtttta tttggaggaa ttaccctagt tttttcgata atgattttca
     5341 taataggttt tttagtaaga aaaaatcaaa aaacagtggt ctatggtatt agtttactca
     5401 ttttatctat tattccattt tttcttttaa cattattgtg ggtaattcct ggagttttag
     5461 gtattattgc gggattgatg cttctaataa gaaaatctta attaggcaat gtgtagacaa
     5521 cgtgaaacga ttaatcaatt aaaagctaaa tacgttttga gaaatgataa cttgtagtcg
     5581 atggaagaca tgtagataaa aaaactacgt gtcttttatt tattctaatt ttttcgaaaa
     5641 aaagacactc tctgattgtt gtattaatgg aaaaggggaa agcagcaagt aaatt
//
LOCUS       DAZKMB010000139         5689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000139 DAZKMB010000000
VERSION     DAZKMB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.142"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG8V3_002554"
     CDS             complement(488..1297)
                     /locus_tag="ACG8V3_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0301026.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG8V3_002555"
     CDS             complement(1297..2043)
                     /locus_tag="ACG8V3_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0301027.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG8V3_002556"
     CDS             complement(2061..2528)
                     /locus_tag="ACG8V3_002556"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0301028.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG8V3_002557"
     CDS             complement(2528..2938)
                     /locus_tag="ACG8V3_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0301029.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG8V3_002558"
     CDS             complement(2949..3962)
                     /locus_tag="ACG8V3_002558"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0301030.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG8V3_002559"
     CDS             4179..4913
                     /locus_tag="ACG8V3_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0301031.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACG8V3_002560"
     CDS             4897..5607
                     /locus_tag="ACG8V3_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0301032.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAZKMB010000140         5481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000140 DAZKMB010000000
VERSION     DAZKMB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.143"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG8V3_002561"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG8V3_002561"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ACG8V3_002562"
     CDS             complement(580..1353)
                     /locus_tag="ACG8V3_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0301033.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ACG8V3_002563"
     CDS             1497..1847
                     /locus_tag="ACG8V3_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0301034.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ACG8V3_002564"
     CDS             1828..2544
                     /locus_tag="ACG8V3_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0301035.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ACG8V3_002565"
     CDS             2544..3992
                     /locus_tag="ACG8V3_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0301036.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ACG8V3_002566"
     CDS             complement(4062..4571)
                     /locus_tag="ACG8V3_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301037.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ACG8V3_002567"
     CDS             complement(4561..5286)
                     /locus_tag="ACG8V3_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301038.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DAZKMB010000141         5388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000141 DAZKMB010000000
VERSION     DAZKMB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.144"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1089
                     /locus_tag="ACG8V3_002568"
     CDS             <1..1089
                     /locus_tag="ACG8V3_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301039.1"
                     /translation="VLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNWEGYENKNP
                     YVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDKKSAETK
                     PLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVYKNFVLN
                     DLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLSEKEQLL
                     YGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVSESSVKQ
                     FFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGLETEKTK
                     IYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1310..3073
                     /locus_tag="ACG8V3_002569"
     CDS             1310..3073
                     /locus_tag="ACG8V3_002569"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0301040.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3157..3615
                     /locus_tag="ACG8V3_002570"
     CDS             3157..3615
                     /locus_tag="ACG8V3_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0301041.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3724..4407
                     /locus_tag="ACG8V3_002571"
     CDS             3724..4407
                     /locus_tag="ACG8V3_002571"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0301042.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4554..5168
                     /locus_tag="ACG8V3_002572"
     CDS             4554..5168
                     /locus_tag="ACG8V3_002572"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0301043.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5184..>5388
                     /locus_tag="ACG8V3_002573"
     CDS             5184..>5388
                     /locus_tag="ACG8V3_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301044.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1915 a          845 c         1109 g         1519 t
ORIGIN      
        1 gtattggatt tttttaaaga aaatgattat ttgaaaaata aagaaatcat tatcccagaa
       61 tttgactcgc ggaacatctt gctagcttta gtacgttatg tcaattggga aggctatgaa
      121 aataaaaatc cgtatgtaga agaagcttgt aacgagttga ttgattatgt agagactcat
      181 ttttttaaaa gaagatattc ggaagatgaa aagtccttta tcatacgagg aatagagatc
      241 gcaatcggaa gaaaggcatt tccccttgga ctgaaagaag aagataagaa aagcgcagaa
      301 acgaagccgt tatttcaatt agtctgtcaa ggattggaag agcaaaaagg aattctagac
      361 ttacaagaag aagatgtcta ttatattttt tctttgttca acagtagaaa ttataccaat
      421 gagaacatgg agctgttaag aaaagatttc gaagttgttt ataaaaattt tgttctcaat
      481 gacctatcac tgaatgaatt gattttacag attattagta tcgctgaaat cgacgatgca
      541 gaaaatttta ttttcaagca atccttttta caatttgtcc gtacgttgtg ggcagatgga
      601 caagtatttt taccggaaag gatctatctt ctatcagaaa aagaacaact tctgtacgga
      661 aaaattgtag aaatattgga agagtggaaa aagaaaaatc aaatccaact aagatggaat
      721 gaaaatttca ttcgaaaatt cgtcaaaagt ctttctttga tcaacagtga acaagcgggg
      781 agacaggaaa tagaaatttt tgttgtttca gaaagttcag tcaaacaatt tttttatcga
      841 acccaatttc atttgtatgc ggaagaaggc atcgctgaaa taaatcctat tttatttcgt
      901 tcattagaag aattgcctga tgaatgtcta tgtgcgaaaa aaagagtggt tttgtgcgat
      961 gctgctagct atcaaaatgg attagaaact gaaaagacaa agatctatcc aatctcatta
     1021 aaaggtgcaa acatccattt gtatcatatt gctcaggaac ttcacctatt aaaaaaacat
     1081 atttcttgat gagaccttaa ttaggagacc ttctacaaag ctttttaatt gtctgtagaa
     1141 ggtttttatt tgagtcctga ttcgtgcgaa gatctactta tataacaagc tttctctgaa
     1201 atttttttaa aatgctattg acttagagtc aactctaagt agtagaatag ataaagtgta
     1261 acaaatctat tttcattaaa agaagatagt acggaggaga gaatgtatta tgagtacagt
     1321 attacatgta ttggatgcaa tcaatagccc cgcagatgtc aaaaaattat ctgttgagga
     1381 aatgaaacag ttggcagaag agatgcgcca aaaaatcttg caaaaggaca gcgatgtagg
     1441 tgggcacgtt ggtccgaatc taggtgtagt agaattaacg attgcgtttc attatgtatt
     1501 tgattcacca aaggataaaa tcgtttggga tgtttcccat cagtcttatg gtcataagct
     1561 tttaactggt cgaaaaaatg gttttgaaaa aggacattac cgtgaagtga gcggatattc
     1621 caaccaacat gaaagtaaac atgactattt tacagtgggg cacacatcta catctatcag
     1681 cttagcaaca ggtttagcta aagcgagaga tctgaagaaa gaatcaggaa atgtggtagc
     1741 atttatcggt gacggctccc taagtggtgg cttagctttt gaaggattga acaatggtgg
     1801 tgttttacaa tccaatctaa tcgtattagt aaacgataat gagatgtcta ttgatgagaa
     1861 tcatggcagt ttatataaaa acttagctga gcttcgtcaa accaacggta aaagcagcaa
     1921 taacctattt aaagcaatgg gatttgatta tcgttacgtg gataatggaa acgatctgga
     1981 aacggtgatc cgtttatttg aagaagtaaa agatattgat catccgattg tacttcacgt
     2041 ccatacggaa aaaggtcgcg gttatcagcc ggcaatcgat aataaaatga aatatcattg
     2101 gcatgtacca tttgatctgt ccaccgggga ggcaaagcaa gtaacttctg gagaaacatt
     2161 taatatgatc atcatggatt atttggataa gaaaatgcaa acaagtacag ctcctattgc
     2221 cgcagtcaat gcagcgatac caggcgcctt tggactgaaa acattccaag aaaaacatcc
     2281 agatcattat tttgacgtgg ggatcgctga acagcactct atcactttcg cttctgcttt
     2341 ggcttctcag ggagtacgtc cagtcgtctt ccaatcaagt actttcttgc aacgggcata
     2401 tgatcaatta tcacatgatt tagcgatcaa cgagaatcca gtgattattt tagtcggtgg
     2461 cggaacgatc tcagctagtg accgaacaca ccaaggaaca tttgacattc cttacttatc
     2521 aagtatcccg aacatcaaat atcttgctcc agcaacaaaa gaggatatga tctctatgct
     2581 agattgggcg ttcgaacaaa cagcgcagcc tgtagcaatc cgaattccta cacatggtgt
     2641 agaacatggt acagctatcg caaatgatta tagtattcca gcctatcaaa cagtaaataa
     2701 tggaggaaaa gtagcaattt tagctttagg cggttttctt gaattaggaa aagaaacagt
     2761 aaaagctttt agaaaaaaag gaatcgaagc aacgttgatc aatccaggaa cgatcacaga
     2821 aaatgacgta gaaacattag ataaattaaa agaaacacac gaattagtca tcacattaga
     2881 agatggtagt ttgtcaggag gatttggcga aaatatcagt cgttattatg gtgattcttc
     2941 aatgaacgta ttaaattttg gtgcagagaa agaattcacg gatagcgttc ctgtagggca
     3001 gctttatgaa cgttatcatt taacaccaaa acaaatcgtt gaagatagtc tgcgtgtctt
     3061 gaatattcaa taattcatta tgctcaccat ccagataatt gtttatacta gtatctggat
     3121 ggttttttca ttataaaaag caaaggggtg gatgttatgt atcagcaagt agaagaatat
     3181 gtcaaaaagt tccaagaaaa atatcctagt tatcaatcag aaaaattagg tttcttttca
     3241 tttggggata cagaaaaaat ggcagacgag ttgagcgaac tagttgtaaa aaagataaag
     3301 actggtacat caagtggtta cgatctgtat aaacaagaga atgagccttt accaaaagtt
     3361 ggtcaattag acgtggtcct gaatggaaaa aatgaaccag tttgtatcat caaaaatata
     3421 aatgtgacca tcttgccttt tagtgaagtc acgaaagagc atgcttggaa agaaggagaa
     3481 ggggatcgga cattagctta ttggcggaaa gtccatgctg agttttttaa acatgcttat
     3541 aaagaagcgt tgaacataga atttacagaa gagaagctgg tcgtatacga agaatttgaa
     3601 gtaattttct tttagtaaga aaggtctgct gaaagaaata gctcattatt gtctcctgaa
     3661 tagaaaaggg ttacattttt atatgaagct gtatttttat ataacattac tggaggagat
     3721 attgtggata cactggaaca aatcaaaggc ggagtcgtcg tttcttgtca agctttggaa
     3781 aacgagccgc tacacagttc ttttattatg gggagaatgg ctttggctgc aaaagaaggc
     3841 ggagcagttg gtattcgagc aaatacgaca gaagatatca atgaaatcaa gcgaatcgtt
     3901 gatttaccgg taatagggat tatcaaaaga gattacgatg ggtcagaagt gtttattact
     3961 gcgacagaaa aagaagtgga agaacttttg gagacgcaag cagaaatcat tgcactggat
     4021 gcaacttctc gtaaacgtcc gacagaaaaa acaactgctg aacttgtcca aatgattcac
     4081 gaacacggtc ggttagctat ggcagatatt tcgacttttg aggaagcgct tcaagcgcaa
     4141 gaagatggtt ttgatctttt gtctacgact ttagcaggct atacagaata ttcacgaaag
     4201 acgcagacac ctgattttga tttattgaat caaatcatag aacaagtcga tatcccagta
     4261 attatggaag gacatactga ctctcctgaa caagtagaga aagcatatga attaggtgct
     4321 ttttcagtcg tagttggatc tattatcacc cgtccgcaat tgatcactaa aagatatgtc
     4381 gaagccgcaa gtaaagcaaa taactaaaaa acacgcacgg cacagattct tgacaaaaaa
     4441 gaaaaattga tagatagttt aaagcagatt ctaagaagat gaaaaaagca ttacttttca
     4501 ttttgtcttc aagatctgct ttttaacgaa ttttatagaa agaaggcgaa accatgtttg
     4561 aacggagtcc ttcagaattg acagaacgtg aaaattacaa gttgctgatt ggttcagtca
     4621 tccctagacc agtagcagta gtaacgacta aatcagcaaa agatatagtg aatatcgcac
     4681 ccttcagtta cttcaatatc gtcagttcga atcgacccat cttatccttg gcgatccaga
     4741 gaaaacaaga tgcagtaaaa gacacagcaa agaatattct tgagacaaga gaagctgtta
     4801 tccatatcct tgatgaggac aacgtagaag aagtaaataa aacagcagcc aatctgccta
     4861 atgaggaaag cgaactgtac ctgactgact tgactttaac tgattcagta atcatttcag
     4921 taccagcatt gacagaagca aaagttcgtt ttgaaacatc cttatatgaa catgtcgaga
     4981 tacggaataa aaataaagta acagctgatt tatttttgct agaagtacag aaatatcatt
     5041 taaatgaaac agtctatgat gataagacag gaaggatcga tccaagaaaa ttgaatgctg
     5101 taagccgttt ggcaggaaat gattatgcag caataggaga aattttcacg attgcacgtc
     5161 ccgaataaaa aggagcacta gttatgttaa aaataagcta taaaccatcg acagattcaa
     5221 aagaaatgaa aaaagaatat gaaacagtga atgattttct gcaagggcag tacttagaag
     5281 ttccgccatt gcaagaccat tttgtcgtta ctactgttac tttagatggt aaggaaatag
     5341 aaatgccaga tcaaacgatt agcggattgt ttaattactt taacaaat
//
LOCUS       DAZKMB010000142         5248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000142 DAZKMB010000000
VERSION     DAZKMB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.145"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8V3_002574"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8V3_002574"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0301045.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG8V3_002575"
     CDS             complement(1297..2133)
                     /locus_tag="ACG8V3_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301046.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG8V3_002576"
     CDS             complement(2317..3534)
                     /locus_tag="ACG8V3_002576"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0301047.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG8V3_002577"
     CDS             complement(3683..3844)
                     /locus_tag="ACG8V3_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301048.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG8V3_002578"
     tRNA            3917..4000
                     /locus_tag="ACG8V3_002578"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5248)
                     /locus_tag="ACG8V3_002579"
                     /pseudo
     CDS             complement(4240..5248)
                     /locus_tag="ACG8V3_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1624 a         1074 c          834 g         1716 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatg
//
LOCUS       DAZKMB010000143         5178 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000143 DAZKMB010000000
VERSION     DAZKMB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.146"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..1100)
                     /locus_tag="ACG8V3_002580"
     CDS             complement(138..1100)
                     /locus_tag="ACG8V3_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0301049.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1102..2541)
                     /locus_tag="ACG8V3_002581"
     CDS             complement(1102..2541)
                     /locus_tag="ACG8V3_002581"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0301050.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2726..4672
                     /locus_tag="ACG8V3_002582"
     CDS             2726..4672
                     /locus_tag="ACG8V3_002582"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0301051.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4936..>5178
                     /locus_tag="ACG8V3_002583"
     CDS             4936..>5178
                     /locus_tag="ACG8V3_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0301052.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1667 a          878 c          856 g         1777 t
ORIGIN      
        1 gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacaagttga caactccttt
       61 ttgtttacaa atcaattgga tatctacaaa ttgtattttc taacgaattt tatagacatt
      121 aaaaaattaa tttaacgtta aatacttcct ccagaaagta ttgagattgg taaaatataa
      181 tccttatttg tcttttcgcc tttaattttt ttcagaagaa tatcaacaat aagacttgca
      241 atttcatcaa ttggttgtct tattgttgtc aattgtggga aaaatttctc aataaaagag
      301 gtaccatcgt atccaataat ttttaaatct tctgggatat taagtttaag ttgatgagca
      361 attttcatag tgagaatggc tgttaaatca tcagaaacaa aaataccatc tggtttgtga
      421 tttataatag ttgactttat ttccatttca cgacggatag tagacagatt attaggaatt
      481 ttgaaaattt ttccatttgg attttgaaaa gaaaaaccta gtgcacgtaa ttctgtggga
      541 gaatccgtgt tatcatttcc tgtaatcata atggtatttt gacaaccatt tttttgtaaa
      601 gtcttagctg caagttttcc cccttcaaag ttatctgaag aaacaatgga aacattagga
      661 gctaaatttc tatcaaaagc aacaataggt gctctcacac gctcataatc agcaattcca
      721 agattatggc ttgatgagat tatgccatca acttggttgg cttcaagcat ttcaatgtat
      781 tctctctctt tagcaggatc attttgacta ttgcaaataa tcgttttata tccatgttta
      841 aataactcta tttcgagata ctcaatgagt tcagagtaaa agatattact aatattagga
      901 aaaataagac caataagctg aggtgacttt ccttgaagtc cacgtgctaa attatttggt
      961 ttataaccaa gagttttcat agcttcatga actttttgtt ttgttttttc tgataaatat
     1021 cccttattat taatgacgcg tgaaacggtt gttgggctaa ctccagctaa ttcagcgaca
     1081 tcagttaatt ttgctaccat tttaatattt taattcaaag tattttccag taggttcgcc
     1141 tgatttgatt tgaataccat tttggtgatt ttcagggaat acacgactag taaacacttt
     1201 ttctccttta ttaataaaaa tctcaatgat tgagttatct acaaaaatgt tagcagttgt
     1261 ttctttagta tcaattgagc attcacgaat cgtaccaaag tcagtggcat attgaatgcc
     1321 tgctttgctg cgatctaaaa taatttttcc ttctttggta tctacaatca agctaagtcc
     1381 atttccttta ctatcagaga aaagaagtaa ctccgatttc tgatttggag gaaaagttag
     1441 ttcaagctca taagtattat ttgtttctgc ttttgctgta aattgttcag aactagcacg
     1501 taatgatgta atagcttcta ctggatattg gtaaagttta ccatctttta ttgaaagttc
     1561 ttttacaaga ctcattgctc cctgataatc atacttatca gttggataat cgatatcagg
     1621 tagtccaatc caactgacaa tcagagcacg accatcagga gtattgaatc cttgagtcgc
     1681 atacgcttca aatccataat caagattttg aatttctgaa gcgcccacaa gttttacttt
     1741 ttctatgtca agcgattgac agatcttaaa agtattcgga tagatattcc cataatttag
     1801 ctcagattta tccaaccctt gaggacaata aatgagaaca ggttttttgt ctacaaatac
     1861 tagatttgga cactcaatca tgtattctga accagtgccg ccaaaatcaa ggttgccaac
     1921 ttcctcccat gtttccacgt tattgtcatc cgctttgtaa agtttgataa agcctgattt
     1981 ttctaaattt tgtgctccaa caatcgcata caattgtcct tcataattaa acatttgtgg
     2041 atcacgaaaa tgatcagtta catcagcagg tttatgaata agaacattct cacctttcgt
     2101 aatgttgtag tctttatcca tccatgctcc gatttgaagt gtatcacgtt cccaattttc
     2161 atttcgaacg tttcctgtat agagtaagaa tagattcccc tctatttcat atgcagaacc
     2221 ggaataagcg ccgtggctat ctcgttttgt gtctggaaga agttctgctc ctgttacatg
     2281 aaaatgaact aaatcatctg attcattgtg tacccattct ttcaacccat gagcagctcc
     2341 aaatggccaa ttttgataaa aaagcgtata ttttccgtta aaaaaagaaa aaccattagg
     2401 atcgtttaac aatcctgttt taggttcgat atgatagcta gcacgccaag gtgatcgttt
     2461 tacattatcc ttaattttat ccatctcatc tttagtccaa tcagaataag cacgatatcg
     2521 tatttctcga ggtaagttca tattttactc cttcaattta tctttttaca actagtatgg
     2581 taaacgtttt ccgttgaaaa atcaatcatt tcatttaaaa aaataaacta tatgacaaac
     2641 gtttgacata tttttaagat atgctaaaat aaatttgcat ttcagtgaac cgttttcacg
     2701 taaaaataaa aaaggagtga ttcgaatgaa ttacagtaaa gtagcttccg acgtaataca
     2761 agcggtcgga aaagataacc ttattgcagc tgcacactgc gctacacgtc ttcgtctcgt
     2821 actaaaagat gacacgaaag tgaatcaaaa agctttagac gaaaatccag atgtgaaagg
     2881 cacatttaaa attgatggac aatatcaagt gattatcggt gcaggtgatg ttaatttcgt
     2941 ttatgacgaa cttatcaaaa aaactggact ttcggaaatt tcaacagatg atttaaaaca
     3001 aattgctagt aaagataaaa aattcaaccc cattatggca ttaattaaat tattatcaga
     3061 tatttttgtt ccaattatac ctgcccttgt tgcaggtggt ttattaatgg cattacaaaa
     3121 ctttttaaca gctgctggac tttttggacc aaaatcaatt gaagaaatgt atcctgcgat
     3181 tgaaggtatt tcctcaatga tccaactgat gtccgctgca ccattcatgt tcttaccaat
     3241 tttagtaggt atttcagctg ctaaacgttt tggagcaaac caatttcttg gtgctgcaat
     3301 tggtatgatc atgacaacac cagatcttgg tggctcaact gaatattgga atatttttgg
     3361 ttatcatgtt tcacaaacaa actatgctta tcaagttatt ccagttttag cagctgtttg
     3421 gatattatct atattagaaa aattctttca taaaaaattg ccatcatcta ttgactttac
     3481 attcacgcca ttattatcag taatgattac tggatttttg actttcacag ttattggccc
     3541 tgtaatgtta ttgttgtcta atggaattac agacattatt gtttggctat ataacactac
     3601 tggatttatt ggaatgggta tttttggagg aacatattca cttatagtca tgactggttt
     3661 acatcaatca tttccagcta ttgaaactca attactttca gcgtggacaa atggtatcgg
     3721 acatggagac tttatctttg tagttgcatc tatggcaaac gttgctcaag gtgctgcaac
     3781 atttgccatt tggttcttaa ctaaaaattc taagacaaaa agtcttgctt cctctgcggg
     3841 tgtctcagca cttcttggaa ttacagagcc tgccttgttc ggagttaact taaaataccg
     3901 ctttccattc ttttgtgctt taattggttc aggaattgct acagcagtag caggattact
     3961 taaagtagtt gctgtttcac ttggttcagc aggtttcttg ggatttcttt caattaatgc
     4021 aacatctatt ccattttacg tattgtgtga attgattagc tttgtagttg cttttgcaat
     4081 tacttacttc tacggtaaaa caaaagctgc tggtatcttt aaagcagaag aaattgctga
     4141 acaaacacca gttaattctt caaaagtagc aacaacagat ccaatatcta ctgatgttga
     4201 atcaacacaa acaaaagttt catcagaaac aattgttagt cccatttctg gtaaagtgat
     4261 tagtctttct tcagtaaatg atccagtctt ttcatcagaa gctatgggaa aagggattgc
     4321 cattaaacca aatggaaaca cggtccattc tccagtagat ggtactattc aactcacgtt
     4381 tgaaactgga catgcttatg gtattaaatc aaataatggt gctgaagttc taattcatat
     4441 tggtattgat actgtatcaa tgggaggaaa aggatttaca caaaaagtta ctactaatca
     4501 aaaagttaga aaaggcgata ttcttggagc atttgaccgt tcaatgattg agaaagaagg
     4561 tcttgatgat acaacaatga ttattgtgac caatacttcg gattattcag aagtaaatat
     4621 agtagctgaa aatacagtca ctgagggtac tgatttactt gaaatcaaat aaatatattt
     4681 ccctgagtaa agatggaatt ttctttccaa aatcaaataa tatagattaa tcaaatattt
     4741 gagaataaca ttttatttaa tcttttcata tatcaattgt atgaaaaatt attagaattt
     4801 taaaaaatga taaaaattca aaaagaaaaa atagtagtta tttacttgtc atgttactaa
     4861 ataaaaaaat aatttaaata tgatatatag tcatttattt agtgatttca ttctaaaata
     4921 aagggagaaa tgtctatgct atacggtgca attgaagcag gcggcacaaa atttgtgtgt
     4981 gccattggcg atgaagaaat gacgatcaag gaaagagtga gctttccgac aacgacacct
     5041 gaggaaacaa tgcctttagt cattgacttc ttcaaacaat accaagcaga tttagcaggg
     5101 attgggattg gctcatttgg gcctatcgat atccatcgcg attccgccac ttatgggtac
     5161 attacctcga ccccgaaa
//
LOCUS       DAZKMB010000144         5162 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000144 DAZKMB010000000
VERSION     DAZKMB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.147"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            228..1369
                     /locus_tag="ACG8V3_002584"
                     /pseudo
     CDS             228..1369
                     /locus_tag="ACG8V3_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1495..2574)
                     /locus_tag="ACG8V3_002585"
     CDS             complement(1495..2574)
                     /locus_tag="ACG8V3_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0301053.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2683..3123)
                     /locus_tag="ACG8V3_002586"
     CDS             complement(2683..3123)
                     /locus_tag="ACG8V3_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0301054.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3180..3590)
                     /locus_tag="ACG8V3_002587"
     CDS             complement(3180..3590)
                     /locus_tag="ACG8V3_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0301055.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3771..4205)
                     /locus_tag="ACG8V3_002588"
     CDS             complement(3771..4205)
                     /locus_tag="ACG8V3_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0301056.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4305..5153)
                     /locus_tag="ACG8V3_002589"
     CDS             complement(4305..5153)
                     /locus_tag="ACG8V3_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0301057.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1885 a          982 c          864 g         1431 t
ORIGIN      
        1 gataggaaaa gaaaaatatc taattttgta ttgctttttt tcttcaagat aaacgtttta
       61 atcgcttctt gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa
      121 atattactgt ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc
      181 tactatctta tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa
      241 ccatcttaat atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc
      301 acacagagga gacagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa
      361 ttacctttca ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac
      421 aaacagcgac catcaaagca aaaattgact attcgggaga cttagcacct atactacaat
      481 cagctgttac atttgagaat gttgatccca ctttgtcgat tggctatgac gagaacactt
      541 tgactatcca acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt
      601 taaaaccagc gctttttact gtcaaggtat cagatggact accgaacgat catcatttaa
      661 cgccgtatag tcagcggcag acaattgagc aagattcagc catcgactca ggaggagatc
      721 ttgttgaaga accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag
      781 aaaatccaga cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa
      841 ctcaaccaga gccagacaat aaatcaaaac ctgaaataac ccctggagaa aaaccagatg
      901 taactccaga accagatacg gattctggaa accaaacagt accggaaaca aatccagaca
      961 cagataatga aactgaaaat ccagaaaaac cagaggtaga tccagaagaa aaaccagatg
     1021 tgactccaga accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa
     1081 aaacaattca agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa
     1141 taaatgaaga gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa
     1201 ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa acgaaaaata aacagctacc
     1261 aaaatccgga gagtcacaaa acaaagttat actgtggagc ggaatcattc tattaagtat
     1321 agcaacaatg ctttctgcaa aaagatttaa acaaaacaga tctctctaac atttgaataa
     1381 tgtctgtgaa cgcatttttc aaataaaaag aaatatccag aaaatgaccc caaaatacca
     1441 gattgaaatc tgatatttgg ggtcgttcat actttatctt tcttcatatt tattttaagg
     1501 gatatctgtt tcttttggaa gatggttcgg gatctttttc actttcaata catctaaaaa
     1561 gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc gttcgctaac
     1621 aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca tcaacttcgg
     1681 attcagcaca taagactcaa ttagatggac aatcaaaatc gtaatcagga tataaacaac
     1741 atctcggatg cctccttggg aataagctat gaaagataga ggaatacaag aaatgataac
     1801 acctgctact gggatcagac ttaaaatgaa aatcatgatc gctaaactca gcagctgatg
     1861 aaagccaaat acagccaagg cgattgtagt gagcaacgta tttaaaagag cgacaacaaa
     1921 ttgagcttct aataccaaac caaatgtatt gacaaactta tcggcaaaat agtaaatatc
     1981 ttgaaagaac caagaaaatt cactttttaa aaacaatctt gagaatacaa ttgttttttt
     2041 cttttcaatc ataaaaaaga aacttaagat gaatgacata gcaaacgcca ctgccatttt
     2101 tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct gttcaaaaaa
     2161 atcgtttcgc tcaatataag tatggatata ttgtaaaaca ggatctgcat cttttggctg
     2221 atgctcgtaa aaatcaataa cggactggat catatcaaat gtttgatgaa taagcatcgg
     2281 gatatacttt gtgaatgcca gatagataag gtaaacgatc agtgtataaa tcatgatagc
     2341 taaaacacct gtagggattc ggacaaatcg ctgaatgatt tgaacaagat gaatcatcaa
     2401 ataagtaaag ataaatgtga gcaaaatggt tgtaatcata cttctaacta agaaaagaac
     2461 aaaaacgaca aataataaaa cagtgagacg gcgtattttt tcattcgcca aaaaacgctg
     2521 ataaacggtc aaatcgacaa cctccaaata acaaaaaata aacacgttat tcattatatg
     2581 atggaatatg aaaataagaa agagaaagag caaaaagaaa aaccgctaaa tgacgttatc
     2641 ttatgagttg gttttcctca taaaatcgtt catctagcag tttcagccct ttttctggtc
     2701 ttgatgaaaa ttatctagca tttttcgggt cagatgttgc agtgtatcca attcttcctg
     2761 ttcaagtgga agataagact tgatcttttg tggaatacct tctgcttgtt tttgcaacgc
     2821 agcaccttga ggagtcagcg taacatttac gattctttca tcacccgttt cacgctgacg
     2881 attaatataa tttttttctg ctaacttttt tagtaaggga gtcagcgtac cagaatccag
     2941 acacaaatca tttcctagct ctttcactga agtctttttc ttttcccata aaactaacat
     3001 cacaagatac tgggtatagg taagattcag cggcttgagg agggaagtgt agtgtctaat
     3061 gatttcctta gaggcggcat ataaaggaaa acatacttga ttaactaaat atagatcttc
     3121 catttttcca cctccgctgt tttactatag tgtatcatag cggatttaca caaaaaagtc
     3181 tattcactaa ctgctttcag agtaacttct atatttccag ctgtagcttt tgaatacgga
     3241 cacactttat gcgctgcctc taataattct tgcgtttctt ccagagaaag accttcgata
     3301 gatccttcaa tctctacacc taagacaaag ctcgtttcac tttctgaata aagggaaact
     3361 tgtgcactga cagtagaagc cccagagata tttttttgct gtttgatcaa ttctaatgca
     3421 ctgttaaaac atgcactgaa accagctgca aacaattgtt cagggttggt tgcattttcg
     3481 acgcgtttac caggagctgc aatattcaat tcgaatgtac gatcgggatt atgaacttct
     3541 ccagagcgtc cgcctgtgtt gatcattttc gtagaataca tttttttcat aaaattacca
     3601 cctttaatta tattttatac aattgaattt tacacaatat aattgtggaa agcaaagaaa
     3661 tggattggca taagataaat aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc
     3721 aaacatatac aaaaaaatat cagagacaaa aactaatctc atcataaaca ttacatatat
     3781 ttctcaaaaa gttgttttct ttcttcaaaa cttacttcac gatggacctg atgaagcatc
     3841 cataaataac caaaaggatc tctgaacatt gagttagtca caccaaagtc catcatttct
     3901 gtcatttctt gaatctctac acagccattt cgtaatgctt tattatgtgt gtcctcgata
     3961 tcagagacga tgatattgaa ccagatcgta tttggatgtt cagcagtagg agcagtcaaa
     4021 ccaagagacg tgttttcatc cagcatatgg aatctcgtgc catacaaggt aaaaatgact
     4081 tcattattgc ctctagggta atctgttgcc tcaatcagtt gtatttcatc aaagattgtt
     4141 tgatacagtt ctagtgcttt caaactatca tttaatacaa aatctatttc cgttccttta
     4201 tgcataaaga tctcctctcc ttttcagtcg aacagttact agattctttt attataccag
     4261 ttaaagaaaa cgcattcaaa aaaatagcgg atttacttac caactcattc gattggatga
     4321 taagtaaatc cgcgtcccat tacttctgct acgctgctga caatgacaaa ggcacttgga
     4381 tcgatttctt ctatcaatgg tttcattcgg gaaaattcat gatcagtcac aacgatcaat
     4441 aaaacctctt ttgactttcg attatacccg ccttttgcct ctagcagggt aaggccacgt
     4501 ccaatctcag cacttaagcg aaggcggatg tcttccaaat aatgttcact catgaccata
     4561 acagatttct ttcgattgat acctgtttcg atataagtca tgacaattga agtgattgca
     4621 atagataaaa tagcataaag aaactcttct attccaaaaa caaacagatt aagagaaaca
     4681 acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca agtggaaata ttttttaaaa
     4741 atgaggggag ggatcgtcgt accgccgcta gaagagttgt ttttatataa aatcgcaact
     4801 cctaaagcaa aaatcgcact tccaaaaatg acagacaaca accgatctga agtaatcatt
     4861 gtttctggaa ctattgcaat gattaaagga aaaaacaagc ttccgaaaag tactttaaaa
     4921 aagggttttt tacctaaaaa tagaagagct agtaccaaca taacgatatt caacactaaa
     4981 ataactatag ctcggtcaaa tcctaaaaca gcttccagta atattcctag accgctgaca
     5041 cctccagcag caatatgatg aggccctaaa aacatattga tagcgactcc taatatgaag
     5101 agggcaaaaa taatcttaat gtatgtccat ataaattgtc tcgtttcttt catttatcca
     5161 ct
//
LOCUS       DAZKMB010000145         5120 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000145 DAZKMB010000000
VERSION     DAZKMB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.148"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..477)
                     /locus_tag="ACG8V3_002590"
     CDS             complement(19..477)
                     /locus_tag="ACG8V3_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0301058.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(490..1821)
                     /locus_tag="ACG8V3_002591"
     CDS             complement(490..1821)
                     /locus_tag="ACG8V3_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0301059.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1832..3274)
                     /locus_tag="ACG8V3_002592"
     CDS             complement(1832..3274)
                     /locus_tag="ACG8V3_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0301060.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3261..4025)
                     /locus_tag="ACG8V3_002593"
     CDS             complement(3261..4025)
                     /locus_tag="ACG8V3_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0301061.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4129..5004)
                     /locus_tag="ACG8V3_002594"
     CDS             complement(4129..5004)
                     /locus_tag="ACG8V3_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0301062.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1547 a         1085 c          751 g         1737 t
ORIGIN      
        1 aacttttttc atcgtacact aaaattcaaa acaactatct ttaatcttta cttcttggtt
       61 ttgttttaat aaatttcctt caaaaatgcc tggagaaact acaatgatca ccgtatcttt
      121 ttgtgcttgc tccaacttct tcaaatctat aatagctaac agtgtccctt cttttacctc
      181 ctggttactt tcaacccgta tctgaaacgg acttccttcg agttcaactg tatctaaacc
      241 catatggatc agtatttttc tgccagattc actttcgatc gttatggcat gtcgggtagg
      301 aaatatatta tcgattattc ctttaactgg cgcatggata tatccatcat gattcgttat
      361 agcgtatcca actcctatca tttttgaaga aaagacctcg tcttttactg ctgttaatgg
      421 aataatttga ccactagcta cagcttttag aatgtttctt gttttaaaga aatgcacttt
      481 ttatcacctc tactttaatg ctggccaata ttttttgttt actttgatca tttcatctag
      541 tatttttttt gcgactctgg catttggtac ggtttgattc aatgtaaatg cttgtaaagc
      601 tttttgataa gaaccttcaa agtaagcatc tactagtaac ttctctactg caacttgtgc
      661 ctccatcaat cctttatgaa aatcactgat tggttttctt agtgtaattg gctccacacc
      721 agttgcacca acatatgccg gtatttcaac aactgcatct gaccgtagat tcggaatcgc
      781 cccttgatta ggaacgatta gcataaagcg acgacgatca tcattcaaca aggaaatagc
      841 tatatcgacg atatattgtc catgctcccc aaaattaaag ttcaatactt cttccacaat
      901 accgcggcgt attttatcag ccatttcttt ggtatttttc tctcgtccat ccatgaccat
      961 attagcacgt gtatactctt tgtcagtatg ctcaacgacc atatcaggat ataagtaata
     1021 ctctaagtaa ttattcggga tattacttgg aaaatttttc gtgatgactg acatcatttg
     1081 aaaggttttt tgccaagttt tatctcctat attaaaatcg gcgacttgca tttcttttgt
     1141 tattagtttt tcaataatct ctggcataat ttctcgttgc aattctttat cataaatcga
     1201 tgtataccag ccaaagtggt ttaaaccata atacgtaacg atccaattct tacgatcata
     1261 accgtaattc actgcaatcg tttcttcaat tgaaatagtc atatcacagg cattaatcat
     1321 ttttacattc ggatatttac gacgaacagc ttcagaaacg atcgactctg gatttgtata
     1381 attcaaaatc catgctttgg gggcatagtc ttgtatatga tcgacaattt ccaataaacc
     1441 tttgattgag cgcatcccat aagcaaatcc tccgagtcca cacgtttctt gtcctactaa
     1501 tccatgttta agtggaatct tttcgtcctt ttctcgcatc tccaatcctc caacacgaat
     1561 ttgtgagaaa acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat
     1621 gcgaatattt gaaaaacctt ttcttttcag cataaattta acaatcaagt acatatccat
     1681 atttctttct tcatcaatat catataaacg tatttcagat aatggcaacc gtttttcatt
     1741 gttcaaaatg gcttgaacga ttcctggtgt atacgtactc ccgcctccag ctatagtgat
     1801 aatctgtttt ttcatcatca tcttttcctt tctattcttc taataattta ttgaattctg
     1861 ccataacata agtcacttct aagccgatga taatttgata atttttttca gttggtttaa
     1921 tgatcccgct tatccctaat tgcttgattc gctgttcctc gaccatggtg ggatccacta
     1981 cctctacccg taggcgtgta ttacaactat agacatccag gatattggct tttcctccaa
     2041 acgcttgtag catttttgtt gccatatatt cataattttt acttgctaac cctaactctg
     2101 tttcttctaa ggttacttca ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa
     2161 tgtttctctt attgatcaaa taataaaaag taacgtaata caaagcaaaa aagaaaattc
     2221 cgattggaag aatcaaaata gcattggtag cgattcgaaa attgatgaga taatcaacaa
     2281 tacatgctcc gaatgtaaaa ccaattcgta tattgaataa gtaacagaca atccctgcta
     2341 aaccggcaaa aagtgcatgg atcacgtaca acataggaga aagaaacatg aaactaaatt
     2401 caacaggctc agtgatattt gcaataaatg aagttcctgc accgcttccc atgattcctt
     2461 tgaccatctc tttattttct ttataagctg ctttatagat agctagacat atagctggta
     2521 taccaaacat cattactaca aaaaaaactg ataaaaacag tccagcctga ggatctccgt
     2581 tgataaagcg tgtcatctct cctctgaaaa cttccttgct aggagatgta tagcttccta
     2641 aatcgtaata aatatacgta ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat
     2701 tcaaaaacat aaatatcccc acacctaatg ctcccatatc aacgagcagt tttgcaaaat
     2761 tatcaatccc aatttggatg agcggccaga taaagcccaa aaaaacagct gcgattgttt
     2821 gtagaatcat caccatagtc aatgggaatt tttcgcccgc aaaataagaa aaaactactg
     2881 gtaatttttg ttctttaaag cgattatacg tccatgccgc tactaaaccg gaaataattc
     2941 caccaaaaat ccccatattc acttctgggt ccataatttc taagcctttt ttcaaggtag
     3001 caatcgctaa aaaaccagta agtgcaggta ttcctttgtc cttatttttg acaaaaccga
     3061 ttgcaactcc tatagcaaat aagatatcca agtttccaaa aacggcattt ccagctgcac
     3121 caattactgg gatgttcagc atatcgtcag ctgataaacg catgatcaat cccgctaatg
     3181 gcatcgctgc aactggaagt agcatcgctc ttccaagctt ctgcacattt gatttaacat
     3241 tcatttttat tcctcctcta ttaaatatcc tcatatgtta ctaactcaat caaaggatca
     3301 tttttcactt tttgagcaaa tgctgaatga attaaaaatt cgacttggtg ggtacgagga
     3361 tacacaaaac ttgaaccatt taacaattcc tgatccaaat acgctgcgtg acacatgact
     3421 tcgatcgttt tatcttctct caatatcatg agtaaacttg ccaaatattc ttccacactc
     3481 gtattgattt gtcgtttttt ttcatcagtt tctcctacga catcgaaaca aggctcaaaa
     3541 taatcgacat gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg caaatcatat
     3601 ttttttgcta atgtaacaac tacctcctga ttttcatcca aagtatgagt atgatgatga
     3661 ctgtctaaat gagttggtat gatccccgca tgataaattt tttgaatctg agcattccat
     3721 tcctgataca gttgatcttt gtcaatgtta aatggatgct gataataaga taaggatctg
     3781 aactctccgc tttcgataag agtatcgaca gttttgagga gtggtttgcc acacgtcaat
     3841 gttaaatgga ctcctacacc tagcctaggc atttcctttc ttagtttcac agcatgctcg
     3901 aaacccggca tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc
     3961 ataatcccat aattcacacc atggctatag ccaaaatcat ccgaattaaa aatcactttt
     4021 cccatttcat tctccttctt ttttctacta aaataggcgc aaccaaataa tcatagtcca
     4081 tttactatgt gtcatttggt tgcgcctata gatagttacg cccttatttt aatagattct
     4141 tgctagatga attgccaaat agatgatttc attttcttga atggcaagtt gaaattgctg
     4201 ttcgatgata tctttaatct ttagtgctgt acaatacgct ttgctatgtt taaatgtttc
     4261 agtaaaaaaa ccaatatttt ctactggcgt ttcttcctga taaatcaagc gtttaaccag
     4321 gaatttgata tgaaccatta agcgatgata agtcagtgat tctttatcat aaacgtttga
     4381 ataggatttt tctagatggg acaaaatcgt ggtcgtcatt tctactattt ttaagctctt
     4441 taaactatta ggctctccta gctcagcatt aatgatatgc atagtgatga atcctacttc
     4501 atttgaccct aactcggttt gatatcgatg attgattcgc tcgaccactt ctactgctat
     4561 atcatattca tttggataaa ggaatctcat ttctggcaac ataattgctg tgacatctct
     4621 atcttctctt tttcgttgaa tagcaaaata aatatgatct gtcaatgtaa tatagatatt
     4681 tgaatctaat ggagaggaga tcctagctgt tatcagttca atcacttctt ccgcaaaatc
     4741 cataaattcg attggaatct gcgagactat ctcactgaat cgtttgatct tttctttaga
     4801 atctaataca aataatttgt cagcatcctc aacatcaaat ggcttactca ctttagcaga
     4861 aaagccaatc cctttcccca tcgcaatttt ttctattcca tcgtcatcga tcaatacagc
     4921 attatgattc agctttttca tcacatacac cgttaattca cctcctctat atgataaaac
     4981 aaaacaactt gcagactcct tcatacatat ttttgtctcc aaacaatttt taaagacaaa
     5041 aaacgaccta agaaagaacc ctttcttagg tcgtgcctaa ttgaatagta gcaccctaca
     5101 taatttacaa aaatagttta
//
LOCUS       DAZKMB010000146         5111 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000146 DAZKMB010000000
VERSION     DAZKMB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.149"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(155..1963)
                     /gene="uidA"
                     /locus_tag="ACG8V3_002595"
     CDS             complement(155..1963)
                     /gene="uidA"
                     /locus_tag="ACG8V3_002595"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0301063.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(1986..3752)
                     /locus_tag="ACG8V3_002596"
     CDS             complement(1986..3752)
                     /locus_tag="ACG8V3_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0301064.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(3765..4559)
                     /locus_tag="ACG8V3_002597"
     CDS             complement(3765..4559)
                     /locus_tag="ACG8V3_002597"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0301065.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(4569..>5111)
                     /locus_tag="ACG8V3_002598"
     CDS             complement(4569..>5111)
                     /locus_tag="ACG8V3_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0301066.1"
                     /translation="NFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILF
                     LIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFA
                     RKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFL
                     YPLSKDKVLENCELLKQKHK"
BASE COUNT         1650 a         1018 c          607 g         1836 t
ORIGIN      
        1 atacaacaat cagaaagccc ctttttttcg aaaaaactag aaaaaaaaca taaaaatatt
       61 tatcattctt taaacagaaa atctttctat aaaaaacaaa aaaaaacgat taggctaggc
      121 taatcgtttt ttgaacattc ttaaaaaatc caaattattt tttataactg aaattgggta
      181 ttgatgacca tcgtttctgt agatagtaag caatcatttt aggttttcta tctctagtaa
      241 agattccttt cttatttcct tgcactctta aaatcccttg acttgttgca aaatctgcaa
      301 aattccacac ttgttcacca acaaaatttt cacatgtatc tattacttcg tgatttgctt
      361 cgtaataagc cacttgatac tcttctgtaa acattgttgg tactgtatca tgaaatccag
      421 ctactgtatc tgctccatat tctgtaaaca ttattggcgt atcaggacat ctttccatcc
      481 atccatctag ctcttttttc aacagatatt ttgcatgtgc cagatcccct cccgaaacat
      541 accaaccata ataacgattt aaacataaaa catcgacaat atctcctacc ttacaaacat
      601 ctggcgttga ttttagataa gtaacgattg ttacaggtct tttctgcgga tcaagttctt
      661 tagttaattc aactaaaggt tcaaaatatt ctttagcacc ttcggaatcc gaatctggtt
      721 catttgcaat agaccacatt acaacacttg ggtggttctt gtcgcgttct atcaattcct
      781 tgatcaccgt tttatgagaa ttttctgttc gtatctcttt ccacgtatta tgtttttcca
      841 cattatcatc taataaagtg gccatgaaat ttaaatgtaa ccctactgca ggtacttcat
      901 caataactac aataccttct cgatctgcta atcgcataat ttcttctgag taaggataat
      961 gtgatgttct aaaagaatta gcacctatcc atttcattaa attaaaatct ttaatattta
     1021 cagcctcatt caaaccacgt ccattaacat acgaatcctc atgcttacca aatcctttaa
     1081 aataaaaagg tttattatta atcaaaaatt tgccattggt aacctgaaca ctgcgaatac
     1141 caaattcttc tgtataggta tcgatcactt tattatcatc aattaaacta attttcatac
     1201 tatataaata agcgtctaga ggttcccaaa aaattggatt cttaagctga atcattcctt
     1261 tctttccttc agcttcagct acaagttgag cctcttcgtc atatatcgct attaagattt
     1321 ttccggtcca ttctgtatcg atattgaatt ctacacttgc atccaaatca gttattcttg
     1381 tattaatagt aatatcactt atatagtctt tcggagttgt atataactta acaggtctat
     1441 gaatacctgc ataattgaag aaatcaaaat taactaagtt ttcctttttt atatccccat
     1501 tttcctgtac tgtttccttc ataatcccca caggtaaagt actatcgtca ataatattgt
     1561 ttacagcaac tgtaattcga ttattcccgt cttttacaaa ccgattaatt tcagactcaa
     1621 atggtgtaaa cccacctttg tgttgagtaa taaactttcc attcaaaaaa acttttgctt
     1681 catgtgttgc agatccaaaa cgaagaacta atctttcttc taataatctt ttaggaagag
     1741 tcacttcttt ttcataccac acccatccaa catgatttct aatgttctct gtttcaaata
     1801 aatcattata agaagaggga actgaaatcg gataactctc tttttcattt attggttcta
     1861 aagcaagttc ttcctgtaag tcatcatttg tatctaattt aaatctccag agaccgctta
     1921 agtcaattac ttgcctcgtc tctgtcataa tgggatacaa catataaacc actcctatct
     1981 aaatattaaa taaatgtatc ctttaaacca caataagcat ctattggact tactccctta
     2041 aattcagaaa gacccattag tttgtccacc aatatttcta aagtttcttg ttttccatca
     2101 taagtattaa tataagtctt tacttgaggt acatcagcca aatgaaacgg acactgtaca
     2161 gatacaaaaa ttgttggaat ttcatgaatg taaaacggta tatccggagt tcctttactt
     2221 gctggccaga caattcgttg aactgtgcct ggattaacat tagctatatt aatgatcaag
     2281 tcatattttt tagttaaatt tttaattggt ctcttagcag catatattcc agctatattt
     2341 ttaatttttt cttcttgcga taatcctttc aattcttctt caggtgaaat gtatttttca
     2401 acttcaaatc cttttgctga caaaatctgt ttcaataaat caatggcttg aggtctattt
     2461 ccagcaacta cttcaccaaa cccacctttt accccatgta catctactag taaaatcctt
     2521 ttatattttt ctggtacaat aggccaaata tcttgtttat ctttaactaa tgtaattgct
     2581 tggtcagcca cctctttaaa aattgattta ttttcaatta aaccaatttt ttccagagct
     2641 tcttgcttag gtaataaaat ttgttctttt gcttttttgt gtaatcccaa tttagctttt
     2701 aaccctaaaa ttctttttaa ggcatctaat aaccgatcat cagtgataat tcctttacga
     2761 tagccttcca tcatataatt aaagtcttca tctggatcat taaaaaatag aaatagatca
     2821 catccagctg caatagaagt gggcaataat tctgatcgtt tcattgcccc agtcattcct
     2881 aacatatgac tagcatcagt aactactaat ccattaaatc cgagttttcc tctaagtaag
     2941 tcagtgatta attcttttga taaagttgca ggaagtaaat cttcatcttt cagatcagga
     3001 ttaaaatgtt ttgaataagc tggcatatgg atatgacctg ccataattga aggtaatcca
     3061 gcttctatta agcctgagta tacttttcca aaagaattat cccattcttc cgtacctaaa
     3121 ctattaatac taaaagatag atgctgatct cgttcgtcaa taccgtcacc agggaaatgt
     3181 ttagctgcag gcaaaattcc actctcctga atacctttca tataagctaa actaagttct
     3241 aaaactttat cagggttttg tccaaaagat cgactagaaa tgataggatt tctccaattt
     3301 ctattaatat ctacaatagg agcaaaactc cagttacatc caatagccga tgcttcaatc
     3361 ccagaaactc tccctaattc ataagcatat ttcgcatcat ttgttgcacc aatttttact
     3421 tctacaccaa cttcagtacc atctgtacaa gctccgttac cacctgcttc cgtattagca
     3481 gcaattaata gaggaatttt actttctgtt tgtaagatat agttttgatc atatacctca
     3541 tcagcagttc ctggattata tcgtacagct ccaatatggt actgattgac catattactc
     3601 aagtaatttt tatcacgact agatcccata ttaacaaaca attgacctat tttttcttcc
     3661 aaagtcatag aatcaatcgt ctcttcaatc caagaaatag catcttcatc taaattatat
     3721 ggtttttttc gtaaatctac ttttatagtc attttctttc tcctctactt attagtaaga
     3781 tttttccaaa aagtatctat atatttttgt tcaatatcta cataatctcc cgataaacat
     3841 ttaaataaaa catcagttct taagttttcc catgattctt tttctttatt gaccaaaata
     3901 ggataaaaga aaactttatt tttatttgca gcttctagat caccaggaga atcaccaatc
     3961 attaatatct tatccaaatc acaaccattt tcaattacac ttcgaataat atcttctttt
     4021 tttcctttat cttgacaaca cagttcatca actaatgata ataattcatg tctttcccat
     4081 tcttcttgaa cagcttcctt attagctgag cttacaataa ttatcttccc atattttttc
     4141 agagctttta tagtatctct agcacttaaa aagggtttat ctaatccttt tgcttgacta
     4201 atctgttgat tgacttcttc cgaccatttt aaagctttta ataaatcctc tgactgagaa
     4261 tattctattt ccctcttcaa agatggatta gacaattctt ttgttagaga tacccagtgc
     4321 tttaatcgat taatcgatgt tacatcattt ttttctaatg ttttaactaa acctaaaaat
     4381 ctgtttattc cacgagtctc tgaatataaa ttaatattat tccaatcctg taggaatttt
     4441 tctctatgat gaacatcaaa aatctctgca gcaattggtc cgaaaaagag attgtgttta
     4501 taagtcatcg tatccataac acagccatct gaatcaatac aaaatatcca attattcata
     4561 tttctctcct atttatgttt ttgttttaaa agctcgcaat tttctaagac tttatcttta
     4621 gataatggat aaaggaaaaa taaaattaat cctacaatca gaaaacaaat tgatggaatt
     4681 aatgttgaag cgttatataa cttttctaaa acatctcccg tttgtacatt tgctaaagag
     4741 tcatagccta taatagaaag agtccaacct ccgacgcctc cagctagagc ttgacctatt
     4801 tttctagcaa aagaatatac cgcatatacg gtcccatctt ctctttgatc agactttact
     4861 tcttgatcat caataacatc agtaatattt gcccaaataa cagtattaaa cacacccaaa
     4921 cccatatagc caaccgaact aagaattaag aatacataca tattactagc ttttatcaaa
     4981 aataagatca aatatactat tgctgaaaag aacattccta ctgatgccag ttcttttttt
     5041 ccatattttt tacttaaaaa caaagttaaa ggcgaaacaa ctattaaaac caaaatagga
     5101 ttgataaaat t
//
LOCUS       DAZKMB010000147         5052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000147 DAZKMB010000000
VERSION     DAZKMB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.150"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..612
                     /locus_tag="ACG8V3_002599"
     CDS             <1..612
                     /locus_tag="ACG8V3_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0301067.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            685..1614
                     /locus_tag="ACG8V3_002600"
     CDS             685..1614
                     /locus_tag="ACG8V3_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFQ0301068.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1751..2509
                     /locus_tag="ACG8V3_002601"
                     /pseudo
     CDS             1751..2509
                     /locus_tag="ACG8V3_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2557..3759)
                     /locus_tag="ACG8V3_002602"
     CDS             complement(2557..3759)
                     /locus_tag="ACG8V3_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0301069.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3839..4363
                     /locus_tag="ACG8V3_002603"
                     /pseudo
     CDS             3839..4363
                     /locus_tag="ACG8V3_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4570..>5052)
                     /locus_tag="ACG8V3_002604"
     CDS             complement(4570..>5052)
                     /locus_tag="ACG8V3_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0301070.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1581 a          868 c          980 g         1623 t
ORIGIN      
        1 atgtatttta tactacctac actttatagc cttttatgtg taatggcacc atgtattatt
       61 tatatggtat ttttttgcaa aaaaaatacc ctgaaacaca atatcctgat tttgattttt
      121 atgctgtact gttggaaagt ttttgaattg actggtacag gtggactaac agatattttt
      181 tatagacctg atacaggtag gaaattttct ctagtacagg gcagtgttaa tcttgtacca
      241 ttcagtggac taaacattag ctttatatta aatatagtaa tgtgtgtgcc cttaggattc
      301 ttactaccaa tcatatggaa aagttatagg aaaatagttc ctgttttctt ggctggggct
      361 ctattttcac taattattga agtctcacaa attataacta caagagcaac agatattgat
      421 gatttaatag caaatacctt tggtgcggtt ataggcttta tactgtgggc tgttttaagt
      481 agagtggtgt ccttgaagga caagtcatct acagtagatt atttagaacc ttatatttat
      541 atattgattg gttttaccgg aaaattctta ctgtattttc ctttctggtt tgcttttgaa
      601 gtattatcct gataattcca tagaaaataa tgagtttgag ttgagaaaga atattcttgt
      661 acacaactct ggaaaggagg aagtatgaaa agattcttaa aatgggggct tgttctttta
      721 gcgtctttga ttgtgattct aatgtgtaca gcggttgtta tttcttatgt gaatcggccg
      781 tatgcaagag ctgtttatga taggattaaa cgcattcttg acaaagattt ttatgaccat
      841 ctatatctag gttcttcatt tgaaataata aaccttgata caatggatat tcatagctat
      901 atgaaaaaag accaagaaga attacccgcc tcattgagca aattatttac gattgattat
      961 gcgcttagtg tcctaagttt agatgaacag attacaatta gtcctactgc acttgacatg
     1021 cagaaagctg actcatctac tgcacatctt caagaaggag atattttatc tgtttatgac
     1081 attatctgtg ctatggtctt accctcagga aatgatgcag cttatagttt ggcaatttat
     1141 acaggtggaa aacttgatcc tgatgcaaag aatgatgaag aacgctatca aagcttcatg
     1201 aaaaacttag atcgttatct aagtgagcaa ggctataaac atactcattt tactgacgct
     1261 gctggctatg actccaatgc tacaacatca gcggaagatt tgacaaaagt agctgcaaag
     1321 ctactaaaga atgataaatt tgcagaaata gttgccaagg aaacttatag agcaaaatca
     1381 attaagggaa atgtttataa ctggaatact acgaatcaat ttatgcaaca agataataga
     1441 tactttactg aaggtgtaaa aggtgtaaaa actggatcgt ttgatgggag atacaatcta
     1501 tgtgtgtatt atacatctag ttctgataac cactatttac ttgtaagttt tggttcagag
     1561 agtgatagta ggcgttatgt agatgttcaa aatctattgc ataatctgcc ttgaaatttc
     1621 gtgcctttaa gcgcgtatct aaagtttctt tgaaaagttt gagaatgaag aaagtgagca
     1681 gtcgaaaaac ggttgctcac ttttttgccc taaaatcgaa aaaatataaa aaagtccttt
     1741 acaacacgtc tcaggaaaaa ctgctaagtc aattctaata tcttgtggag caagacttgc
     1801 accttttgat ataagagagc ttagagaact aatgagcgaa gatgaactag aactcgataa
     1861 aattggagat agaaaaactg ccttgtttgt aataatatca gatacagatg atacatttaa
     1921 ttttgtagtt tcaataatgt attctcaatt atttaatcta ctatgtgata aggcagatga
     1981 tgtgtatggt ggaagacttc cagttcatgt taggtgtcta cttgatgagt ttgcaaatat
     2041 cggtttgatt cctaaatttg aaaaactaat agcgacaatt cgttcaagag aaatatcggc
     2101 aagtataata ctgcaagcac aatctcaatt aaaagcaatt tataaagacc atgcagatac
     2161 aatagttggt aactgtgact ctacactttt tctaggagga aaagaaaaaa caacagtaaa
     2221 agaattatca gaaaccttag gaaaagaaac catagaccta tataacacat cagaaacaag
     2281 atcaaatcaa aaatcttttg gtctaaatta tcaaaagact ggcaaggaac ttatgagtca
     2341 agatgaaata actgtaatgg atggtggtaa gtgtatttac cagctaagag gagcaagacc
     2401 ttttctatct gataaatttg atattacaaa gcataagaat tacaaattgt tggaagatta
     2461 tgataagaaa aacttgtttg atgtagaaga gtatttaaaa caaagatagc gtaaaatatt
     2521 ttgtgtaaat aactcgtcct tctgcaaaat aatgggttac tcagtaaaca ttgaagctaa
     2581 tgtatccgtc acttgttgga agcctttatg gcttctattt aagaattttt gattgtatgt
     2641 atcaaagatg gatactagga aacgctctag tgattcttca ttttgaaact gctcttttct
     2701 gtggctgtat ttcttgattt gcttattaaa ggattcaatc aagttcgtag agtaaatagt
     2761 cttgcgtatg gctaatggaa aatcatagaa agtgagcagg taatcgttcg aaaggatcga
     2821 ttccaccatt tttggataac tggttttcca tttttccgca aaagcaccac gtgcttccaa
     2881 tgccgccttt ttagatgacg cttgatacac cattttaaaa tcatcacaga cttccttacg
     2941 atcatcgaca cgcactttgt gactgatatt acgggaaacg tgcacacagc aatgttgaaa
     3001 acgggctttg gggtagaacc gctgcacggc ccctgccatc ccctttaagc catccgtgat
     3061 gaaaaggagg acgtttttca cgccacgttc ctggaggtct attaaaatct cttcccaaat
     3121 cgtgatagat tcagttggtg caatcgcata gctcaacact tcctttgaac cgtccgggcg
     3181 aatgccaact gcgatatgaa tggcttcctt ggcgacggtt ttccgcttta aagggatata
     3241 ggttgcgtcc atataaatag cggcataacg gtcatgaagc tcacgttcct taaatgaagt
     3301 gacttcttcc gtaaatgatt tagtcatatt ggacatggtt tggggcgtgt agtgatgccc
     3361 atacattttc tcaatcaagt ctgcgatttc cgacatggta ataccttttc ggaagaggtg
     3421 aatgacggtc tcctctaagg tatcattcgt ccgtctataa gcaggaacag tctgttgctt
     3481 gaactcgccg ttgcggtcgc gcggaatctg gagatgaagt tccccgtact cggtcttgac
     3541 cgtacggtca taggagccgt tacgagaatt acccgtgtta aaaccaatgc gatcgtactt
     3601 ttcgtaatcg aggaaagccg ttaactccgt tttgagaagc gtattgacag ctttttccaa
     3661 atggacacgg aataattcat tcaaatcgcc tttattcgct agagtcttta gaatttctgt
     3721 agtaaaatcg ttcatatgga agtcctcttt tctgtgaatg tgttgtggta actctattct
     3781 actgaaggga cttccttttt tctatttaca caaaatattt tacactctcg gattgaaaga
     3841 acgcggtctt tcaggagtaa agatggtcat ctcggatgcg catatagggc tcacaaaagc
     3901 tgtgaaagaa taccttcttt gtgcgagcta gcagagatgc caagcacatc tccttcgcaa
     3961 catttccgac aaattctcca agaaaatctc aagcgatatc aaagaagact ttaaagccat
     4021 cttcaaaacg gcaagcatcg agctggctgg agaacgtaaa caacttatta tggataaata
     4081 tggggaagac aagaaagtcg caactgcctg taaaatattg gatgagggct tcgaagatgc
     4141 catgcaagta atggttttac cagaaaatat ccgtcaacga ttgagaacaa cgaatgttct
     4201 tgaaagacta aatgaagaag tacgccgtcg tgaacgtgtt gtccgaattt tcccaaattg
     4261 tgattctgtt attcgtatca tcggtgcttt attgatggaa aaggatgacg aatggacttc
     4321 tgcacctaga aaatatcttg aatttgatcg taacgagatt tagcacatta agccgccaat
     4381 agtataatat actggtgaga agtatcaaga gacgcttcgc tttttgctct tgacacttct
     4441 taccagcata ttggaccgta gctaggctca acaaggcaaa acatcgtact ttgagatttc
     4501 ctttttacac aagattttgg acttgacaca gcccctatgg ttcttacctt gtttatgctt
     4561 atttaaatct tactttatta cagtactttc aaacttcgtt ttgtaagccg ggtattttga
     4621 tatttcataa ttatgtccct ctttttgaat atattctttg atatctgctt gttcatctac
     4681 actttctttg cgtaaaatac gttcgatcat tgagcagcag tgcatgtaaa gcaagaacaa
     4741 ttgattgttt gaaaattcga tccccattag tttttcgagt tctttgacgg ccttttgtac
     4801 caatctcaaa accttcattg catcaaggat cgttaaattt tctacgatct tgttaatcga
     4861 gaaattaaag tattgagcac aattataggc gataaatgga gcatttgatt ctatcacctg
     4921 ttgttccttg gcgtattcat aaattttttc tgccaaaaag gtttttcctg aaccgcttac
     4981 acccatcaat aaaatcggta atccgttatg aggatacaag acagccgtct tgatttgatc
     5041 gatcgcttcc tt
//
LOCUS       DAZKMB010000148         5029 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000148 DAZKMB010000000
VERSION     DAZKMB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.151"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..2005
                     /locus_tag="ACG8V3_002605"
     CDS             71..2005
                     /locus_tag="ACG8V3_002605"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0301071.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2462..2878
                     /locus_tag="ACG8V3_002606"
     CDS             2462..2878
                     /locus_tag="ACG8V3_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0301072.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2892..3458
                     /locus_tag="ACG8V3_002607"
     CDS             2892..3458
                     /locus_tag="ACG8V3_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0301073.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3481..4467
                     /locus_tag="ACG8V3_002608"
     CDS             3481..4467
                     /locus_tag="ACG8V3_002608"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0301074.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4622..>5029)
                     /gene="scm"
                     /locus_tag="ACG8V3_002609"
     CDS             complement(4622..>5029)
                     /gene="scm"
                     /locus_tag="ACG8V3_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0301075.1"
                     /translation="SASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSD
                     SSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPK
                     TGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         1535 a          926 c         1103 g         1465 t
ORIGIN      
        1 taataagtat caaactaaaa gaattatcat atattcatta tatagatagt ctataaggag
       61 cggatgatca atggcaaagc aaaatattgt cgttgtagga gcaggatatg ccggtgtttc
      121 agctacaaaa tacttggcaa agaaactgaa aaaagaagat gtagcaatta ctctgatcga
      181 tcgtcattcg taccatacta tgatgaccga gttgcatgaa gtagctggcg gacgtgtaga
      241 accagaagca atccaatacg atctgcagca tttgtttgct cgtcaaaaga atgtacaact
      301 tgtcacagac actgtaatcg gtatcgataa agaacgaaaa atcgtcaaga ccaaattagg
      361 ttcctatcct tttgatcagc tgatccttgg aatgggcgga gagccgaatg actttggtac
      421 acctggtgtc aaagcacacg gattcaccct ttggtcattt gaagattctg taaggattcg
      481 ggaacatatt gagaagacag tagcaaaagc tgcattggaa ccagatgatg aactgcgtaa
      541 agccatgctg actttcgttg tctgtggttc tggatttacc ggtattgaaa tgattgggga
      601 actgatcgac tggaaaaatc gcctagccaa agattataaa ttagatccaa acgaaatcac
      661 actgatggtc gtagaggcaa tgccgacgat cttgaatatg ttggatcgaa atgatgctgc
      721 aaaagctgaa cgctatttgc gtaagcacaa cgtggaatta ttattgaatg caccaatcgt
      781 agaagtggct gctgatcata tcaaattgaa agacggacgc aacattccga ctcacacact
      841 gatttggaca gctggtgtca aaggaacttc tgacgctgct gactttggat tagaatcagc
      901 acgcggacag cggttgatcg ccaatgaata tatgcaggca aaaggctacg aagataaaaa
      961 catctatatc attggtgatt tagtctttta tgaagaattc ccaggaacac cgacaccgca
     1021 gatcgttcaa gctgctgaac aaacaggaca cacagccgca gcgaatatcg tggcttcgat
     1081 caaaggcgga gaaaagcata aatttaaagg aaactatcaa ggatttatgg tttctattgg
     1141 tgccaaatgg ggggtagcta acctatttga taagatccat ttaagcggat ttttagctat
     1201 cgtaatgaaa catatcgtga atttgaaata cttctttgat attcgttctg gttattatat
     1261 gttccaatat ttaatgcatg agtttttcca tatcaaagat gatcgcaacg tgactcgagg
     1321 acattcttct cgttacggca atgtcttatg gagtgtgcca cttcgtattt tctatggttt
     1381 agtttggttc gttgaagctt ccaaaaaaat cattgggaat ggtgaagtgt tcaaaccaag
     1441 tacttggttt ggtgaaggat catggttcac tggaaacagt gtgttccctt ggacatggga
     1501 atacaatgta acagatgttg ccagtggtgc ttctgctgct ggtgatgcaa ccacagctgc
     1561 cagcggagca ggggcaagtg ccggagaagc agcgactcaa gctgcacatt ttggtttgag
     1621 ttatgcatac ggtgaagaac caatggctgt gattgagaag tcaccagact ggttcacaaa
     1681 gatcatgcaa gtgatcatgc caaacaatga tgtagcaatg ttcttccaaa aattcatggt
     1741 attctttgaa attgccttgg cattagcatt gattgctgga ttattcacat ggctatgcag
     1801 tgcgacaacg attgtattgg tgatcacgtt ctgcttatca ggcatgtttt actgggtaaa
     1861 catttggttc atttttgttg ctttcgcatt gatgaacggt tccggacgtg cgattgggtt
     1921 ggatcgctgg gtaattccat ggctacaacg tacactaggt aattggtggt acggtagacc
     1981 aagttcccag tacggcaaga aataaataga taaagaacgt tatttgaatg caagatttca
     2041 atagaaaggg gctgagacaa accgtcacag cctccttttt atgtaaaatt aaaggaataa
     2101 gatgcccgag ataagcctcc atgagcaaga cacaggtgtc acaacctctg ttacaacctc
     2161 ttgttataaa cggtgttcca gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa
     2221 acttgaaaag taattttcgt aaattccttc ttatttcttg aagttgccca cttctgtcac
     2281 aacctcttgt tataaacggt gttccacctg ctgcttctcg gtaataagcc caaagaatta
     2341 gaaaacatgg aaagctgttt tctattcttc gttcttatta ctcaaagcaa gacacaggtg
     2401 tcacaacctc ttgtttttat gctaaaattg ggtgagttaa tctggaaaaa ggagaacaaa
     2461 aatgagcctt aaagaatttg ctgaaaaaag tctgctcaaa ccttgggatg tttttattat
     2521 tttgcttttg actgttcttt cttttttacc agtcatgatt tttggtcttc agcaaaacac
     2581 cgcaacagca gaaaaagaag cggtactgcg tgtggatggc acagaaatca aaacattccc
     2641 tttgatggct ggagaagaga gcaacacata tacttacaaa gatgaacacg gagattataa
     2701 cttgatcgag atagatggcg atcgagtacg gataaaagaa gcagattgcg atgaccagat
     2761 ctgcgtacgt cgaggctggg caacgaaaaa tggcgaaacc atcgtctgtt tgccacataa
     2821 attagtgatc gaagtccgtt caacagatgg aggtgacgaa gacagtctga tttattagac
     2881 cttcacacac catgagtaaa ttacaaaaaa tgatttatat ttcgctactt gttgcacaag
     2941 gagtgatcat tggtttattg gaaaatatga tcccttatcc tttttctttt gcgcctggtg
     3001 caaaactagg cttagccaat ctaatcacga tcgttgccat ttttaccatg aaaaaacgag
     3061 acagcttctt actactttgg ctacgactat tcctgactac tttgctaggc ggaacgattt
     3121 ccacattcct ttatagtatg agtggtgctt tgcttagtta ttttggaatg ttattggtca
     3181 agcaattagg accaaaacgt gtcagtatca tcggaattag tgcggccggc ggtttcatgc
     3241 ataatgtcgg acagcttttg atggcttctt tcattgcaca atcatggaca gtcatgttgt
     3301 acttgccagt cctttcagtt ttagggattt tatcagggat tgcaatcggg atcgcagcga
     3361 attatctttt acgacatgtg cagacacttc aacgattcca aaaagattat gagcagggaa
     3421 ccaaacaaaa atggagccca atgtttacca aaaaataatc gtcacacaag gagttttttt
     3481 atgaagctac atccaatgtg ggaaaactat cctgctttaa gtaaagaatt gacagaaaca
     3541 ctaaaacgga tggaagaaac tgtccgcctg aaaaacaaac ctgtcgaaca agcaatcaaa
     3601 gaaacgatcc atgccggcgg aaagctgttg cgtcctgcct atcagctatt gttctcacaa
     3661 tttggaccag agcaagatcg agacaaagcc atcgctttgg cagcttctat cgaaatgctc
     3721 catacggcca cactgatcca tgatgatatc gttgatgaag cggatctgcg acgtggtcag
     3781 cctaatatcc gtagtcagtt tggtaatgtc gtttctgtct atgcaggaga ctatttattt
     3841 gtctgctgtt ttaaactatt gtctgattac tccacttcct tgaaaagtct ccaattgaat
     3901 tctcgcagta tggaaaaagt attgaatggc gaattagggc agatggatga tcgctataat
     3961 tatgaacttt ctgtaaaaga atatttggaa aatatttcag gtaagacagc tgaactgttc
     4021 caattaagtt gttctgttgg cgcttttgaa agtggaacaa gcgagcgctt cgcaaaaaaa
     4081 gcaggagata tcggactttc tatcgggatg gcctttcaga ttatcgatga tattttagat
     4141 tatacaaaag agagccagga aatcggcaaa cctgtactag aagacatgcg tcaaggggtc
     4201 tattctttac cgttgatcta ctctttgcaa aaaaacaaac caaagctact accttattta
     4261 gagaagaaag cggcaatgac agaagaagat gtcgacgccg tacgcaaaat cgttgaacat
     4321 acaggaggcg tggaagaagc taggaagtta gcggcctctt acacagaaaa ggctttgaag
     4381 gaaataaaga aattacctgc cacctctctt cgaacaaaag agaatctctt ttcccttacc
     4441 caactgattt tggatcgcaa agactgaata gggaaaggtt cgatacgtaa ataaatttct
     4501 cagaaggaaa tctacgtgtt ttttgacaaa ttgaattgta gttcttgtat ttatgataaa
     4561 aagcaaaaat aaagagcaac gtccgttagc gacgttgctc ttttttatct tgaatatttt
     4621 tttacctttt cgatttctta taactcataa aagtgattct taaaccgatt acaagtaata
     4681 taacgcctgc gagtgttatc caattattcg attgacttcc agttttagga agtgctcttt
     4741 ttgatttgtt tccattgtca ttgcttgttt tattagttaa gttaaagttc gtcctgttat
     4801 ccgaacttgt ttttgagaag gagacacttg catgacgtgt atcgcttgtg gtagacgacg
     4861 tgtctgtact atcgcttgaa gttgatgaat ctgagctatc acttgtggta gaggatgtat
     4921 ctgtactgtc acttgaagct gacgtgtccg tgctatcact tgtagtggta gatgaatctg
     4981 agctatcact tgtggtagag gtagtatctg tactgtcact tgaagctga
//
LOCUS       DAZKMB010000149         5012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000149 DAZKMB010000000
VERSION     DAZKMB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.152"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8V3_002610"
     CDS             complement(<1..182)
                     /locus_tag="ACG8V3_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0301076.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8V3_002611"
     CDS             517..951
                     /locus_tag="ACG8V3_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0301077.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8V3_002612"
     CDS             974..1294
                     /locus_tag="ACG8V3_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301078.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8V3_002613"
     CDS             1435..1626
                     /locus_tag="ACG8V3_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301079.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8V3_002614"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8V3_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0301080.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8V3_002615"
     CDS             2112..2375
                     /locus_tag="ACG8V3_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301081.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8V3_002616"
     CDS             2399..2587
                     /locus_tag="ACG8V3_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301082.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8V3_002617"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8V3_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301083.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8V3_002618"
     CDS             3070..3441
                     /locus_tag="ACG8V3_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0301084.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8V3_002619"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8V3_002619"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8V3_002620"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8V3_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301085.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8V3_002621"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8V3_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0301086.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1745 a          784 c          980 g         1503 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg ga
//
LOCUS       DAZKMB010000150         4939 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000150 DAZKMB010000000
VERSION     DAZKMB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.153"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..696)
                     /locus_tag="ACG8V3_002622"
     CDS             complement(334..696)
                     /locus_tag="ACG8V3_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301087.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="ACG8V3_002623"
     CDS             complement(770..1150)
                     /locus_tag="ACG8V3_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0301088.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1232..2407
                     /locus_tag="ACG8V3_002624"
     CDS             1232..2407
                     /locus_tag="ACG8V3_002624"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0301089.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2484..3068)
                     /locus_tag="ACG8V3_002625"
     CDS             complement(2484..3068)
                     /locus_tag="ACG8V3_002625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0301090.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3269..4267
                     /locus_tag="ACG8V3_002626"
     CDS             3269..4267
                     /locus_tag="ACG8V3_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0301091.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4379..4888
                     /locus_tag="ACG8V3_002627"
     CDS             4379..4888
                     /locus_tag="ACG8V3_002627"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0301092.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1569 a          923 c          857 g         1590 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tgtttcctcc taatatttgc atacttcata ataataataa cataatacct
     3121 aacctctcgt aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta
     3181 taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct
     3241 gctatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc
     3301 aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat
     3361 cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat
     3421 ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa
     3481 acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg
     3541 tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat
     3601 tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc
     3661 atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa
     3721 agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat
     3781 tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt
     3841 atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc
     3901 aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat
     3961 tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct
     4021 ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac
     4081 ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact
     4141 gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa
     4201 tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc
     4261 tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt
     4321 tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat
     4381 ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt
     4441 aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt
     4501 gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa
     4561 cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc
     4621 gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt
     4681 gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt
     4741 gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt
     4801 agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc
     4861 agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta
     4921 gacgagttct ttctttttt
//
LOCUS       DAZKMB010000151         4933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000151 DAZKMB010000000
VERSION     DAZKMB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.154"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG8V3_002628"
     CDS             85..972
                     /locus_tag="ACG8V3_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0301093.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8V3_002629"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8V3_002629"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0301094.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG8V3_002630"
     CDS             2274..2720
                     /locus_tag="ACG8V3_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0301095.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG8V3_002631"
     CDS             2993..4345
                     /locus_tag="ACG8V3_002631"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0301096.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG8V3_002632"
     CDS             4516..4803
                     /locus_tag="ACG8V3_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0301097.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAZKMB010000152         4823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000152 DAZKMB010000000
VERSION     DAZKMB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.155"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..313)
                     /locus_tag="ACG8V3_002633"
     CDS             complement(20..313)
                     /locus_tag="ACG8V3_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0301098.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(341..1006)
                     /locus_tag="ACG8V3_002634"
     CDS             complement(341..1006)
                     /locus_tag="ACG8V3_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0301099.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1146..1778)
                     /locus_tag="ACG8V3_002635"
     CDS             complement(1146..1778)
                     /locus_tag="ACG8V3_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0301100.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1785..2852)
                     /gene="liaS"
                     /locus_tag="ACG8V3_002636"
     CDS             complement(1785..2852)
                     /gene="liaS"
                     /locus_tag="ACG8V3_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0301101.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2849..3580)
                     /gene="liaF"
                     /locus_tag="ACG8V3_002637"
     CDS             complement(2849..3580)
                     /gene="liaF"
                     /locus_tag="ACG8V3_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0301102.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3749..4228)
                     /gene="greA"
                     /locus_tag="ACG8V3_002638"
     CDS             complement(3749..4228)
                     /gene="greA"
                     /locus_tag="ACG8V3_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0301103.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4364..>4823)
                     /gene="mltG"
                     /locus_tag="ACG8V3_002639"
     CDS             complement(4364..>4823)
                     /gene="mltG"
                     /locus_tag="ACG8V3_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0301104.1"
                     /translation="MVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEGVKENDRK
                     NIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTGYGPGPFD
                     NPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         1367 a         1022 c          734 g         1700 t
ORIGIN      
        1 tgtgtcaccc tctctttttt tattgttttt taaagtgata ggttggctcc tccaggttgg
       61 gatcataatc aacaagtaaa tcatatcctt taaagaacca ttcatctgct tcctcgataa
      121 aaaaaagtgt cccatcaatt tccactttct ttacaggatg ttctggctgt tcaactgaca
      181 ttccaatcga aaaaccatca tgaacctctg ttttgccgta gacttttcca aaaaaacgta
      241 ttccgtaagt ttctggcaga tcaatttctg agtcaaacca cttttgtgct tttggcgtta
      301 ccgttaattt catcccaaca acctactttc tcttttcttt ctattgattc atcttatcgt
      361 ctagaatatc cacatcgcct gtctctccaa taaccagtag attatcattt tcacgaacat
      421 attcatctgc tgcaggagaa gcgataaccg tttgatctac tcttcgtatg gcgactactg
      481 ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt tttgttgaaa aatttaggat
      541 tggagactcg gatttctgct aaagaatatt tatctgacaa ctctaggtaa tctaaaatat
      601 tctttgacac taaattatgc gcaatcctta ttcccatgtc tcgctcggga tggacgacgc
      661 gatcagctcc gatcttttca agtactctgg catgatactc attttgggct tttgctaaaa
      721 tatttggtac gcccatttcc tttaccatca gagtaactaa gatactggct tgaatatctt
      781 ctccaatagc gacaattaca tgatcgaaat ttcttatgcc taatgatcga agtgtcattt
      841 catcctgtgc atttgccaca acagcatgag tggctatatt catatattca ttgacccgat
      901 cttcattact atcaatagcc agtacttctt gaccagcttc gatcagtgtt cggcagatac
      961 ttccgccaaa tcgtccaaga cctacaattg caaaattttg tttcaccgtt ttcctccttt
     1021 agtatcttct tttctcagta taacaaagcc tttacaaaaa caaaagcaag catcattatt
     1081 gtaaaaaagc caagaaaaat aatagttatt ttctcagctt ttgattatcc tctttatcat
     1141 cgtttttatt tcaccaaacc atgtttgaat gcatagatag cggcttgtgt acggtcttcc
     1201 acttctagct tagccaatat attggaaaca tgtgttttta ctgttttcag tgtaataaac
     1261 aattcgtccg ctatttcctg attacttttt ccttcagaaa tcagcattaa aatttcattt
     1321 tcccgattcg ttagttcttc atgtaataca ggctcatttc gccgactcat tttttccatc
     1381 atttttgttg tcacttctgg ttctaatact cgctcgccac gttgcgttgc ccttatagca
     1441 tcagcaattt catgtgcggt agaggtcttc aacaaatacc ctgctgcacc tgcttcaatg
     1501 gcaggataaa ctttttcgtc atcaataaaa ctggttacga taatgatttt agcttctggc
     1561 caatctttta agattgcttt tgttgattcg ataccatcca tttcttccat gactaagtcc
     1621 attaaaatga cgtccggtcg aagtgtcatc gccttctcat agccttgtcg tccattttca
     1681 gcttctccaa ttacttcaat gtcttcttga atagataaat aaaaagagac gcctaagcgc
     1741 accatttcat ggtcatctac taataaaact tttatcatcc ttatctatgc ctccttcatc
     1801 aaagggacct tgatttctac acttgtacct tgacctttaa aactaatgat ttttactgtt
     1861 ccaccaatac cagctactcg ttctttgata ttgttcaatc cataacttcc cgttttcgtt
     1921 tcattcatat taaaccctgt tccgtcgtca ataatacgta acaaaagatt attgtctatt
     1981 ttgtgcaaat atacctctaa ttcattggct ttagcatgtc ttaatgtatt tgataacaat
     2041 tcttgaacga ttcggaacaa gtgatcctca atggaactag atagttttac atcttctaca
     2101 tcccatttca gcgaaatctg gattttattt tgcaattcct tcaataactg ctcgatacct
     2161 tgctttaaac ttttctcttc taaattgact ggacgtagat gaagaagcag cgcacgcatc
     2221 tcagactggg aagcgttgat gatctctgct accattttca actgcttgcg aaacatttct
     2281 ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca tcatcatcat tgctgcaaac
     2341 aattgttgcg aaactgaatc atgcagctca cgggctaatc gatgtctttc taattctaaa
     2401 atttcttctt tggtttgtcc atctacatat tggggacggc tcgttacaat ttgtaattca
     2461 ctagatactt caatcatttt ttcttttatc ttagtgattt ccttatcaat atctttgatg
     2521 tataaatcgt ccgatgcact tgggatcgga agaagcaaca atttggattc ataattgcca
     2581 gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt ttgtcttttg aataagtgat
     2641 aataatagaa agacaattaa tcctacacct aaggaaatgg ctaacaaatg gaagataagc
     2701 ggaacatata ataaacgtgc acgcagtaat tctccccacc aatgactttg attgctggca
     2761 taaaaataag tgaaaagtga aaatagaata ataaggaaag ccgcaatccc cgaataaaca
     2821 gctagcatcg ctctggatat ttttcccatc atattcgtat cacctcgaca tctccaatca
     2881 aggtattagt aataatcttc aatctacgag ggttttcatc ataatcactg ctgtagattt
     2941 tgatttgttc atttttcaaa gaaaattgct cttcttcaaa caagacattt cctactaatg
     3001 tcgcatgctc taatcggatg gctacgccca aaggtactaa gatccgtgtt ctgcctatcc
     3061 cttttcttac aatcacaata ttatcatctt ttggcaaaag cgtattgccc aaatctatga
     3121 tagtatcacc agaaatgacg gcgatgttga tatcgtccca ttcatagacg tcgtttccaa
     3181 tacgttggtt accgaataat tgctgtcttt tccgctcatt attatgtgtt tttaattgct
     3241 ctgtttggat catcatgatc tgtttctttc gccaaaaagc atttttggtt aaatctatcc
     3301 ctgaaatctc tacacctttc agaccaataa ataaaacggc aaatacagcc atcatccata
     3361 aagcaggact gttgactaag ctaaaaaaaa tgaccaaact tcctaatatc aattgaaaat
     3421 tttgaaattt agtacgagga tgtttgcgca tagctagata tatattgaaa ataccgaata
     3481 tcactaaaag caaaagtcca gtattgttta cgatttgcca aacagcaaat aacagcagta
     3541 gtgcttcaac taccacaaaa aaacgccaag aactattcat cgctctttct ccctcctgac
     3601 acattctcat ttcatcatac ctattttttt aactgttggc tataggactt tagatgtaag
     3661 atcgtttgtt gaaatctaaa atacacaaag agaaaaagat ctagagtgta tcatcagtaa
     3721 gatgaactct ctagatcttt cagtcagact acgcttgttc tactttgacg attttaacta
     3781 acatatcgcc acctggagta gcaatagtta cttcatcatt tttacgtttg ccaatcaatg
     3841 cttgcgcaat aggagaatca ttggaaattt tgccagaaaa cggatctgct tcagcgcttc
     3901 cgacaatcgt gtactcttct tcttctccat ctggtagttc gatgaatgta actgttttac
     3961 caattgatac ctcatctgca tctacaccgt cgttatcgat gatttgagca aaacgaatca
     4021 tattttcaat tgttgtgatt cttccttcaa caaatgcttg ttcatctttc gctgattcat
     4081 attcagagtt ttcggaaaga tctccgaaac cgcgggcaat tttgattcgt tcaatgattt
     4141 ctccgcgttt gactgttttt agttcttcta attctttttc taatttttct ttaccttcta
     4201 gagtcattgg ataaactttc tctaccatgt gtaacgtctc cttttttcga taaaataccg
     4261 caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt accacgcttt tcttcaaatg
     4321 taaatgtttt tctaaaaatg atcaatgtct agagcctgtt tttctaagaa ttatttacat
     4381 atttttccac taattccata tgttcttcat aagttttagc gaaataaaca tttccagtgc
     4441 tcgtatctgc tacgaaataa taatagtcat tttctgctgg ttctaatact gccttgattg
     4501 cctcttcgct tggattgtca aaaggtccag gtccatagcc ggtattggta tacaaattat
     4561 atggtgaatc tacagctgta tcttcatacg tcactaattc tttgtgttca cctaatgcgt
     4621 ataaaacaga gatatctgac tgaagtggca tatttgcttt gattcggtta aagaagactt
     4681 gtgcaatgtt tttacgatca ttttctttta cgccttcttt ttcaactaga gaagctagag
     4741 ttaaaacttc ttgtacagtc aaatttttct gcttgatagc tgaataatag ttttgcatga
     4801 ccgtattcgt cttgttcacc att
//
LOCUS       DAZKMB010000153         4734 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000153 DAZKMB010000000
VERSION     DAZKMB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.157"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8V3_002640"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8V3_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0301105.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACG8V3_002641"
     CDS             724..1878
                     /locus_tag="ACG8V3_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0301106.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACG8V3_002642"
     CDS             2093..3061
                     /locus_tag="ACG8V3_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0301107.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8V3_002643"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8V3_002643"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0301108.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACG8V3_002644"
     CDS             4091..4699
                     /locus_tag="ACG8V3_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0301109.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1457 a          909 c         1113 g         1255 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttg
//
LOCUS       DAZKMB010000154         4451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000154 DAZKMB010000000
VERSION     DAZKMB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.158"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(456..881)
                     /locus_tag="ACG8V3_002645"
     CDS             complement(456..881)
                     /locus_tag="ACG8V3_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301110.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1255..2226
                     /locus_tag="ACG8V3_002646"
     CDS             1255..2226
                     /locus_tag="ACG8V3_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0301111.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2258..3655
                     /locus_tag="ACG8V3_002647"
     CDS             2258..3655
                     /locus_tag="ACG8V3_002647"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0301112.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3814..4437
                     /locus_tag="ACG8V3_002648"
     CDS             3814..4437
                     /locus_tag="ACG8V3_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301113.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1556 a          715 c          908 g         1272 t
ORIGIN      
        1 tactacgtga tgtagaataa gcctttctat cgtttggtta ttttatattc aaatatttga
       61 aataaataaa tctttactct ttgatttgta aactgaaatg atccactgtt tgttttactg
      121 atgaagcttg atcaaaggaa gaagctgagt ttgaagttca taaaaatcag ccatagtgag
      181 ttttgcttgg atttgtttat cttcttctac tttttacata attttgaaca ggataagatt
      241 caaatgtttt aaatgaatct ttgactagtt tgtgacagtt gtcgcatatg ctcaagcttc
      301 cttggtcatt aaattctgta tggatatttt tgttgttatt acatgtacaa taatcaggac
      361 tatcgatcat ccatttcgtc tcctttacaa tcatcttgct tctttactat agcagaaaag
      421 aaggggagaa acaaaagaag atccaacaga cggcactata gactgtagat gttgccatcc
      481 tcatccttca aacggtaatt accgattttt gtaaatatga ctgttgtttc ttttttattt
      541 tcttttcctg aaataatata ttcgtcatct ttggtagtga tcttgatatc tgtcagcact
      601 gttttttctt cagatgattc agaagaatca gttccttgtg tctgatacgt gcgtgtccat
      661 tccatttttt tatctgtaaa tgtcaactgt cccgcggata aatccatatc tttacgtact
      721 tttagcgtat atacactgtt ggtggtatct gctttttccg cttttaagac agaagaatcg
      781 gttgtttcgt ccgaggattg gtcagagccg ccaaacaaat cacagccagt caatgtcaaa
      841 agtcccgcag acaaaataaa tagtacgatt aatttcttca aaatacttcc cccatttgat
      901 aggataaaga aattatacca ttggaatgta aaacttcaaa cggatggaag agaaaaacgc
      961 acaaaacacc cacaaataga gtaaatttta gaataaaatt ttaaaaaggc aaaaaatccg
     1021 ccactttcag aaagcgtttt aaaaaagtgt tgcaatcttt taaaaaaatg ctatattaaa
     1081 aatgaaccgg ttcaaatact tgtttaagta taattaatag tgagaggaag ttgctctact
     1141 gaagacttta gttatatgga tattgatgac aagcaacact atgcaaagaa tgcggtcatc
     1201 ataatcgaac aacaagaaag agaaatcaaa tgtaactggc aggagtgaga atgtatggca
     1261 tcgatccgtg atgtggctaa gatggctggt gtttcagtgg gaacagtttc ccgttacttg
     1321 aatggtcagc aactgaagga aaagaacatg aaaaaaatag cagaagcgat ttcagcgttg
     1381 gactataaag aaaacatcat tgctaaaggg ttgaagaata atcgaagctt ctctattgga
     1441 cttttgataa acgggatttc cagccgtttt gggtcggaag ttgtctcagg gatcgaaaga
     1501 gtagctgaag aaaatgggta tagcttatta ttaagcgggt ttgccgatca gccagaaaaa
     1561 attgaacaaa aaattgaata cctgatgaag catgtgatcg atgggctgat tgtcttctta
     1621 tccgaagaag agtggtctgg atttgaaatg ctcacaaaaa tgagtattcc agttatcgca
     1681 ttgaattgtc cagaaggacc agcaggtatt gatacaatat tagtgaatga tcgagaaagt
     1741 gtagcaaaag tgatcgctca tcatggtgaa caaggacata aaaaaatcgg atttattgct
     1801 gccactcaaa cagactacgt agcaagagaa cgtttagcag gagccaaaga agcggtccaa
     1861 aatgatccat cgatccaatt agaggtcttt acaggagact attctagaaa aagcggctat
     1921 tacggggcaa aagcattatt agaaaaaggg gcaactgcta tttttgtcag caattacaat
     1981 atgtcgattg gtgccatcga atgtttcaat caagaaggtg tcagaatcgg gaaggatatt
     2041 gcgttcagtc actatgatta tttagataaa aacgatctga gtatccttcc gaaaatcacg
     2101 atcacgccac caacagataa aatcggggaa cgagcagcag aacagctgtt aaaacgaatc
     2161 aaaaaagaag aggaagaaaa tgaaaaagtg attgtgatgg aaaatatcat acatggaatc
     2221 aactaaaacg aactaaaaaa gaaaaagagg tgctttgatg aaaaaatatc aatttccagc
     2281 aaatttttgg tggggttctg cagcaagtgg cccacaaaca gaaggaagga tttcaggcga
     2341 tggaaaagga gagaacattt ttgattattg gtacaaaaaa gaaccgcaaa agttctttga
     2401 tcaagtagga cctgaaaaaa cctcgcaagt ttatacgaag tatcaagagg atgtacagtt
     2461 gatgaaacag actgggcata attcatttcg aacatctatc caatggagcc gtttgatacc
     2521 tgatgaatct ggaacaatca acgaagaagc cgttcgtttc tatcatcgtt attttgatga
     2581 gttgattgaa aacggtatcg aaccctttgt gaatctgtat catttcgata tgccgatgta
     2641 tctgcaagaa aaaggcggat ggttgaaccg agaaacggtt gaagcctatg aaaaatatgc
     2701 caaaagttgt tttgaattgt ttggttcgaa agtaaaaaaa tggtttactc ataatgaacc
     2761 aatcgttcca gtcgaagcag gttatttata tcgatggcat tatccagaag agtcagatat
     2821 gaaaaaagcg atccaagttg gctatcatga agcgttggct tcagcgttag ccatcaaatc
     2881 ttaccatgaa atggagcaag gtggtgttat tggtatcatc ttgaatctga caccaagcta
     2941 tccaagagac gaatcaaacc ctgaagatgt taaagcagcg caaatagcag atgcgttctt
     3001 caatcgttca ttccttgatc cagctgtaaa aggcgagttt ccaagtgaat tgattgagat
     3061 agtaaaagaa cttgatctca tgcctgtgat ccatgcagag gatttgacgg ttatcaaaga
     3121 aaatacagta gatcttttag gtatcaatta ttaccagcca cgccgggtaa aagcaaaaga
     3181 aacaccaatc gatactaaaa acggtccaat gcctgaggat tatttcgaca attatgaaat
     3241 gcctggtcgt aaaatgaatc cttatcgcgg atgggagatc tatgaacaag gaatctatga
     3301 tattttgatc aatgtacgag atcattatgg aaatatcgac tgctttattt ctgaaaacgg
     3361 tatgggtgta gaaaacgaag cacgatttat caaagaagat ggcatgatcc acgatgatta
     3421 tcggatcgaa tttgtccaat ctcatctgaa atatgtccat caagccattc aagaaggtgc
     3481 caattgttta ggctatcata tgtggacatg tatggataac tggtcgtgga ccaatgcata
     3541 taagaatcga tatggcttta tttcagtaga cttagcacaa gatggaaaaa gaacaatcaa
     3601 aaaatcgggt cattggttca aagacgtatc agataaaaac ggatttgagg cttaagaaag
     3661 aatagttgag agcaaatgcg tgaaattctc cattcttacc attttgtgaa aaagaagttc
     3721 aattatttac gatctttttc aacttttttg tctttctatt tcattttttg tatataatag
     3781 aatagtcaaa attaaatagg agtggaggaa agcatgagga aaaaaagacc gtttcttctc
     3841 ttttggttat tcggtttttt cttactgctt attttatccg cagcattttc aggaaaaatg
     3901 acagcagatc ttgctcagaa agaaacagaa aaaatcgtag caagcaaaga atcagtagat
     3961 aatttcaaaa aagaattctc aacttattgg tgggcggagt ataaaataac tgccaattac
     4021 gaaaaaggcg aattggacat ttatttgcca aaaggaaaag aagaaaatca atttattaat
     4081 atcgatgacg tacttagtgc gatttctctc agacaatatc aatttaaaga caagcatttg
     4141 accgttttgc ttttgactca tgatggcgac aaagtagccc gtttgaaaaa tggaaaagtg
     4201 atcaccgctt attctgaaaa agtagacaaa aaagtggtcg ccgatcgttt agcggaatgg
     4261 aaaaagacaa atgaacaagt caagaaaaca actgtctctt ctcaaaaaaa cgagtcgaca
     4321 ggaacaagtc cttcagattc tggcagtgga caaacgataa cgggaagtga agaaaatcaa
     4381 gcctcaatta ttacgaatga gtcatcacaa ggacaatatc aatcctatgg acaatgaaaa
     4441 acagaggttg t
//
LOCUS       DAZKMB010000155         4451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000155 DAZKMB010000000
VERSION     DAZKMB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.159"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACG8V3_002649"
     CDS             <1..792
                     /locus_tag="ACG8V3_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0301114.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ACG8V3_002650"
     CDS             889..3138
                     /locus_tag="ACG8V3_002650"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0301115.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..4316
                     /locus_tag="ACG8V3_002651"
     CDS             3141..4316
                     /locus_tag="ACG8V3_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0301116.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4319..>4451)
                     /locus_tag="ACG8V3_002652"
     CDS             complement(4319..>4451)
                     /locus_tag="ACG8V3_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0301117.1"
                     /translation="QNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1550 a          641 c          815 g         1445 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctagagtaac ccgtggtgct agttaatttt
     4321 aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga cttaatagaa
     4381 cactgtaagc cagtaatatg ctttcaaatc tactctctag ccctttaaca gaacgtgagt
     4441 tgaaattttg a
//
LOCUS       DAZKMB010000156         4425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000156 DAZKMB010000000
VERSION     DAZKMB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.160"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG8V3_002653"
     CDS             690..1658
                     /locus_tag="ACG8V3_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0301118.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="ACG8V3_002654"
     CDS             1726..2484
                     /locus_tag="ACG8V3_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0301119.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG8V3_002655"
     CDS             2552..3262
                     /locus_tag="ACG8V3_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0301120.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACG8V3_002656"
     CDS             3298..>4425
                     /locus_tag="ACG8V3_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0301121.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZKMB010000157         4402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000157 DAZKMB010000000
VERSION     DAZKMB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.161"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..925)
                     /locus_tag="ACG8V3_002657"
     CDS             complement(29..925)
                     /locus_tag="ACG8V3_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0301122.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(918..1598)
                     /locus_tag="ACG8V3_002658"
     CDS             complement(918..1598)
                     /locus_tag="ACG8V3_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0301123.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1599..2381)
                     /locus_tag="ACG8V3_002659"
     CDS             complement(1599..2381)
                     /locus_tag="ACG8V3_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0301124.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2374..3291)
                     /locus_tag="ACG8V3_002660"
     CDS             complement(2374..3291)
                     /locus_tag="ACG8V3_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0301125.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3455..3709)
                     /locus_tag="ACG8V3_002661"
     CDS             complement(3455..3709)
                     /locus_tag="ACG8V3_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301126.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1291 a          952 c          767 g         1392 t
ORIGIN      
        1 tctactagtt ctcactaatc ttgtataatt aaaccctccg caacttgacg gagatggaga
       61 agatgccccc gtctaatcta gcggtcatct ctcctcccat tccttccacc aatttttttg
      121 acaggtaaag cccgattccg ctagattccg tatttgtgcg gctggtattt tcggtataaa
      181 aacgctggaa gactttttca acagaggttt gcgttcctgg tttgatagga ttggccactg
      241 ttaatcgaat gaatggatcc tcttttgaaa atgtcacctt tgcttgtcca tctgcataac
      301 gcaataaatt ctgtatgata ttttgcagca ctcgcagcaa catgagcgga tctgctaata
      361 cagttgtatt tatcgagtgt tcagcgaacg ctacctcgat atttttctca gaaaattgtt
      421 cataaaaaga gaaaaatact tcttccacct ctttagaaaa agaaagcgta tggagatcca
      481 tttctttttg cttcgtctct attcgagcaa gatcataaaa ctgatccgtc atactgagta
      541 atcttgcaac agattgtttg atgatcgtta aatacttctc ccgtttctcc gtatcctcta
      601 cctgttccag taattgtaca taaccgttaa tagcagtcaa aggagttctg agatcatgag
      661 aaaggccgac gatcgatagt ctgatgtttt cttccatttc ttgaaatgct tgtccttttt
      721 gttcattttc atccaaccat gtattcagtg catctactag gtccattaga ttcttttctc
      781 gaaattcgac tgaaagacga ccaccatatt cttgtcgttc agaaagagtg gcgattttct
      841 tagttaatct cttgatttcc ctttgcagga agaaaataaa acttcccata aagataacaa
      901 cgagaagcaa cagccattta atcaaatcta aaccccactc cccacaccgt atgaatatac
      961 acagcatgtg cctttgctag cttccctcga agattggaaa tatgcatatt cactgttttc
     1021 tcactatcaa aatatggttc ttgccaaact gtttcataaa gatttgctcg cgaaaatact
     1081 tttttcggat gttctaaaaa aagcttcagc agatcgaatt ctcttcctgt caaatggaca
     1141 gccacaccat tcacatttac atccctcgac tctttgtcca gtaagatttc cttgtaatag
     1201 atgcgttgcg acagatcttt tacttggaca gaagtctgat gacgaagttg gatgccgatt
     1261 cgtaacaaaa gttcttcgat atcaaatggt ttcgtcacat agtcatccgc tcctgctgcc
     1321 aacaaggaaa ctttatctct ttgatcgctt tttgctgaaa gaacgatgat cggaacattc
     1381 gatgtctgtc ggatttctgc taacaatgct tcacctgaaa gccctggaag catgagatct
     1441 agaaggatca aatcgacagg atatgtgctt aacaataatt ttccttccgt acctgaataa
     1501 gcatcaagaa gtttgtatct ctgttcaaga acctcctcca gtaaggaatg aacagcttca
     1561 tcgtcttcga taatcaaaat agtttccagc aggaccacct acttaatgtc agatttttgg
     1621 aaaataaaca ttcctagagc tgatgatacc acgattgtta ctagactgac cagaatcatt
     1681 ttcggtgctt gcgaataagt tgcttgttgc gcaaagtccg tatacatgag ccacgtttcc
     1741 gtaaaccact ccgttagaaa ttccaagcct ttgatttttg cgaaaacacc gggaatgaag
     1801 gccgttccca tgatataaag aaaagaaaga agcaaactga cactggtact tctagtgatg
     1861 aatgtcaaag ccactggaac agataatgtc cctaatgtca gcagaagcaa tacaggaaat
     1921 acttttagaa cataatttcc atagtttttg attccctcaa cttcaccaaa tccgaaagca
     1981 agtgtgtaag caagcatggc tagtcccgtg atcaaaacca cgccaagtgt tgtgatcaaa
     2041 agcgtaacga gaagtttcga cacataataa gtcattcgat tccgtcctaa agataacgta
     2101 ttgcgaatcg taccgttttg aaattcttcc ccaatataaa agcacgcaaa tgctgcgaaa
     2161 gccagaagaa tccatgcagc cattttttct accattaaaa tccctgttgc accgttgatg
     2221 actgtatccg ttgcttggtt taattcattg ccgtattttg tatgcatata ccattcgttc
     2281 caagaataaa aaagcggaag tacgaccatc agtcctagtg agacccacat tttacggctc
     2341 ttccatactt ttattttttc agcagttaat agattaaaca tgtggattgc ctccagttaa
     2401 ttgtaagaaa taatcttcca gtttttgccc aactaggtga ataccatcca caggaacttt
     2461 tgcttctacc aatgcttgat tgatggctgc tacttggtca attccttcaa aaagctggat
     2521 aacacggcta gattgtacaa aataatccct ataaccaaga gagtcgagaa ccgttactgc
     2581 ttgttccgtc gccgaagtgt caatttttat aaattgacga gattcttgtg ccaattcttc
     2641 tttcgataat tctttgatca agtgtccttc atgcaaaata ccatagtgag tggcgatctg
     2701 tgacaattca tctagcaagt ggctagaaag caagacggtg atgccccgtt cttttaccaa
     2761 acgttggatg atttctcgca tttctgccat ccctgctgga tccatcccat tcgtcggttc
     2821 atccaaaatc aaaaattctg gattcgtgat cagtgcattt gcaatagcta aacgctggcg
     2881 catacctaaa gagaaattct ttgctttctt tttccctgta ttttccaatc gcatcatttt
     2941 cagcaggtct tcgatttctc tatccgacac accgccgttc gctgcttgga tacgtaggtt
     3001 atctcttgca gttaattctg gataaagagc aggggtttca atcgattgac ccatacgtcg
     3061 tcttgctgcc tgcaaatccg tcgttccaaa taattcgatc gaaccttcat caatcgagat
     3121 caaacccata atagttctca taaaggtaga cttacccgct ccattttccc caatcaatcc
     3181 atagatcatt ccacgtttga tttcgattga tactttatct aatgctttgg cagcaccgta
     3241 ttttttggtt ataccagttg ctttcaagac cgtttcattt ccaaaatcca tacttatcct
     3301 tctttcttat tgatgagtga agtatagttg acgggtataa agaaagcagc cagaaatgta
     3361 taaagaaagt gtaaattttc caattgaatg tcaaaaaaaa caatcggaca atacgtgtct
     3421 ttcctattat caggcagttt gttatacgct tgtatcagtt tcctgattct ttcttttcga
     3481 ccaaagtcgt ttctctgtat tcctcttcac tgaaaatttt tgcttctttc agatcaagct
     3541 tgagtaattt taccagaccg gttataagga taactatagc aagactccct cccagaaaaa
     3601 ttagactgtc tcgcagacgt ggttgcgttg tcttccaatc aaagagcgca ccaaacagtg
     3661 ctaatccaag tggactggcc accatggtaa tcgttgttat cgttgacata agtcgtcctt
     3721 gtatatgcgt tggaacttct cgctgaagcc agaccatata aaaagcaaaa gaaaacatta
     3781 atccttaaaa acggacttat gaaaaataat acatgccgtt tgggatttac aattagctac
     3841 caaataagag gttatgacaa aagacttttc acaatccctt cttccgaata aacggtgttc
     3901 tcctgccaac tcctcggtca caagtcacaa tatttatcaa acatgagaag caatttttgt
     3961 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcagaa gcggataaat
     4021 aatattgttt gatgatactt tatctttttc ccgtttgttt atagatcgaa tgatattata
     4081 gaccaagaat cctacctatt aacatagcta aaaacacaaa aaaggcactt tccaaattgt
     4141 ggaaagtgcc agaaaagtta ggattaaggt aactgcgctc agcattgctt cgcccagtac
     4201 gattctcgta actcgtgaac tcgaacgata atcgcaatta acgttttgag aactgtgaag
     4261 ctttacgggc tttcttaaga cctggttttt tcttcgtatc attgaataag tttgaaaaac
     4321 cttgatttat aaaggttttc ccgtaaatat attttattat atttgattaa atttgaaatg
     4381 tttgtgttct aaaatgtgtt ct
//
LOCUS       DAZKMB010000158         4377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000158 DAZKMB010000000
VERSION     DAZKMB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.162"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG8V3_002662"
     CDS             73..234
                     /locus_tag="ACG8V3_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0301127.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACG8V3_002663"
     CDS             253..1530
                     /locus_tag="ACG8V3_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0301128.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACG8V3_002664"
     CDS             1540..2196
                     /locus_tag="ACG8V3_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0301129.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACG8V3_002665"
     CDS             2196..3572
                     /locus_tag="ACG8V3_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0301130.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..4115
                     /locus_tag="ACG8V3_002666"
     CDS             3702..4115
                     /locus_tag="ACG8V3_002666"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0301131.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4140..4377)
                     /locus_tag="ACG8V3_002667"
                     /pseudo
     CDS             complement(4140..4377)
                     /locus_tag="ACG8V3_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1699 a          522 c          761 g         1395 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DAZKMB010000159         4369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000159 DAZKMB010000000
VERSION     DAZKMB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.163"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACG8V3_002668"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACG8V3_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0301132.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="ACG8V3_002669"
     CDS             complement(2944..3378)
                     /locus_tag="ACG8V3_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301133.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..>4369)
                     /locus_tag="ACG8V3_002670"
     CDS             complement(3395..>4369)
                     /locus_tag="ACG8V3_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0301134.1"
                     /translation="RYYLFGPFRANNIDKVFFKLKMNNLNVAMADRERLYNSLQNLTL
                     YSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIY
                     LFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTS
                     ITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLIS
                     SVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIML
                     KSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT         1345 a          816 c          602 g         1606 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatct
//
LOCUS       DAZKMB010000160         4336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000160 DAZKMB010000000
VERSION     DAZKMB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.164"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..340)
                     /locus_tag="ACG8V3_002671"
     CDS             complement(<1..340)
                     /locus_tag="ACG8V3_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301135.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFI"
     gene            complement(548..1474)
                     /gene="glsA"
                     /locus_tag="ACG8V3_002672"
     CDS             complement(548..1474)
                     /gene="glsA"
                     /locus_tag="ACG8V3_002672"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0301136.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1497..2924)
                     /locus_tag="ACG8V3_002673"
     CDS             complement(1497..2924)
                     /locus_tag="ACG8V3_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0301137.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2955..4169)
                     /locus_tag="ACG8V3_002674"
     CDS             complement(2955..4169)
                     /locus_tag="ACG8V3_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0301138.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1369 a          922 c          770 g         1275 t
ORIGIN      
        1 aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa taataactta
       61 aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa agagcaatac
      121 catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt atacaatatt
      181 ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc acccttagag
      241 aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca cacagataca
      301 cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca cgcctccagt
      361 ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc tttttattta
      421 gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa acaagctcac
      481 aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca gcctctttca
      541 agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc aacaattgga
      601 taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct atgccgtaac
      661 gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt acaccaacat
      721 ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt gatttgacga
      781 ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt cctttatttg
      841 ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag cattgtctga
      901 aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct ttcatatagt
      961 aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt tcttcattca
     1021 atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc tcaaattttt
     1081 catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag
     1141 gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg aaggcaaaac
     1201 cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct ttgattgcta
     1261 ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc
     1321 cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt ttgggatcca
     1381 cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag tggaaatttt
     1441 ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt cattttttat
     1501 ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat aactaagacg
     1561 ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc tgcgatacta
     1621 gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc taaacaagca
     1681 acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag actgtcatct
     1741 ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag gtagattaca
     1801 acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct taatgtaaag
     1861 acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc aggaacaccg
     1921 tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc tacgtgaagt
     1981 gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa acaaacaaga
     2041 atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa taggaattct
     2101 aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt agtagcgacg
     2161 gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa cattgctaat
     2221 ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt ttctacgccc
     2281 atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga aaaatcagga
     2341 atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac ataatggatc
     2401 cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc gatccgagca
     2461 atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca gaaaataatc
     2521 aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc cgttgcatag
     2581 gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat ctcaaaccat
     2641 tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac ccaagtatag
     2701 acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa tgccacaggg
     2761 ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc tgaggtcgcg
     2821 aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa aaagccaaag
     2881 aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct tcctcttttc
     2941 tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa tccttcttct
     3001 tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa aatcactgtt
     3061 gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat ccctaagaag
     3121 taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc tatcagaatc
     3181 gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc atcccattgt
     3241 ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat cccaatcagc
     3301 atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa tccagcaacg
     3361 gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc aatgatcagc
     3421 caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt gactgcttgt
     3481 tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca aagcagtcct
     3541 gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa aggaatcttt
     3601 ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt gtggatgact
     3661 gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc ccatatccag
     3721 tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat atagcctttt
     3781 aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac gataaacatc
     3841 agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat gttggggaag
     3901 cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc gccgatatcc
     3961 cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat cccaacggag
     4021 agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc gccgtaaaaa
     4081 aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat gaatgctgta
     4141 tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg attacgagta
     4201 taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt gcatttataa
     4261 aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt cacacaaata
     4321 aagacaaaaa ataaac
//
LOCUS       DAZKMB010000161         4310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000161 DAZKMB010000000
VERSION     DAZKMB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.165"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..1285)
                     /locus_tag="ACG8V3_002675"
     CDS             complement(65..1285)
                     /locus_tag="ACG8V3_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0301139.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="ACG8V3_002676"
     CDS             complement(1305..1916)
                     /locus_tag="ACG8V3_002676"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0301140.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="ACG8V3_002677"
     CDS             complement(1959..2891)
                     /locus_tag="ACG8V3_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0301141.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="ACG8V3_002678"
     CDS             complement(3312..4172)
                     /locus_tag="ACG8V3_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0301142.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DAZKMB010000162         4296 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000162 DAZKMB010000000
VERSION     DAZKMB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.166"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..503
                     /locus_tag="ACG8V3_002679"
     CDS             75..503
                     /locus_tag="ACG8V3_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0301143.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ACG8V3_002680"
     CDS             490..732
                     /locus_tag="ACG8V3_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301144.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ACG8V3_002681"
     CDS             complement(1032..1247)
                     /locus_tag="ACG8V3_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301145.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="ACG8V3_002682"
     CDS             1354..1668
                     /locus_tag="ACG8V3_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301146.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="ACG8V3_002683"
     CDS             1772..2950
                     /locus_tag="ACG8V3_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0301147.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(3131..3316)
                     /locus_tag="ACG8V3_002684"
     CDS             complement(3131..3316)
                     /locus_tag="ACG8V3_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301148.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            3353..3646
                     /locus_tag="ACG8V3_002685"
     CDS             3353..3646
                     /locus_tag="ACG8V3_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0301149.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3734..3880)
                     /locus_tag="ACG8V3_002686"
                     /pseudo
     CDS             complement(3734..3880)
                     /locus_tag="ACG8V3_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1466 a          647 c          824 g         1359 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc ttccaaattt
     4261 aagcaatact tcaattaatc aagttttatc tttttt
//
LOCUS       DAZKMB010000163         4288 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000163 DAZKMB010000000
VERSION     DAZKMB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.167"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..525
                     /locus_tag="ACG8V3_002687"
     CDS             88..525
                     /locus_tag="ACG8V3_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301150.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            620..1504
                     /locus_tag="ACG8V3_002688"
     CDS             620..1504
                     /locus_tag="ACG8V3_002688"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0301151.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1726..>4288
                     /locus_tag="ACG8V3_002689"
     CDS             1726..>4288
                     /locus_tag="ACG8V3_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0301152.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1403 a          722 c          963 g         1200 t
ORIGIN      
        1 tacttagaat ccagccaccc tagatatttt tctgtataat cttaaataaa gaaaacgata
       61 ttaaaaaatg cacgtagaag tagataaatg gagtggtttt gcaaatttct tttatttcta
      121 gaagttattc acgtatacgg atttgttttt ctcacaaata aaaaggcagg aagtacaatg
      181 gatcatttgt atataaaata tattcatcaa ttttccgttt tggtattagg cacactaatt
      241 atagcgggga ttgttgcatt tattattcaa ggtcgtgaca tattaacagt acatcaagag
      301 tttttcagtt atgcatttgg tattcctctg atcgctataa ggtctgataa caaaaagtgg
      361 aagaaacgct attacattct cttgctattt ttgtgttttt atctgccacc taagattttt
      421 actttattag gtatacaatg cctatcattt tcttttattt cctatatcat tgttttaatt
      481 ttcctcagtt tcatagtatg ggtattttat cttgtcaaaa aataaatgaa tgtttcatga
      541 acagtacttc atcacagcct aatttttttc tttatgctat cgttatatta aagaaatcaa
      601 tcagaaggga aagaaagtga tgaaaaaagt tttattagtg gtaaacccca gttcaggcgg
      661 tgaacaagca aaggaatttg agcaattagc gatagcaaaa ttggaatcgg tgtttgatga
      721 agtggtcgtc ttgcatacaa aaaaagcagg ggatgcaaaa aactttactc gcgaagcggc
      781 tacggaaggg tatcatagtg tgtttgtcat gggcggagat ggaacagtca acgaaggaat
      841 cagtggaatc gctgaacaag aacatcggcc aaatttcgga tttttcccat tgggtacggt
      901 gaatgacttg gctcgagcat tagggatacc gctagaacct gaggaagcga tcaatcattt
      961 ttcgattgaa tcagtcaagc cattggatat tggaaaaata aatgacgact attttatgaa
     1021 tgtcgtcgca attggctcta tccctgaagc aatcaatgat gtagatgcgg agaagaagac
     1081 gaaatttgga aaactagctt atttcatgtc aggaatcaaa cagttagctt ccacacaatc
     1141 ctactcgttt cacgtagaag ttgatggaaa gaaggaagaa attgagagca gtatcttgct
     1201 gataggacta actaactcag taggcggttt tgaaacatta cttccaaatg caaaagtgga
     1261 tgatggtaag ctccatttgg tttatttaaa agattcttct ttgatcgata cgcttaaaac
     1321 attgccagac ctattaaaag gagtagatga atctacgaaa aatctagcct atcttacttg
     1381 tgagcaatta gttgtgtcat tagctgatga ggaagaattg acgacgaatg ttgatggaga
     1441 tgaaggggat aaattacctg tgacaatcaa agtgttaccc tcacatttac aggtttattg
     1501 ttaaaatgga ggttgtgacc gaagagttgt caggaggaca tcggttgttc ggaataaggg
     1561 actgtgacaa gacttttgtc acagcctttt tttgtacaaa aaagaaattt tcacctaaca
     1621 gaaaaatttg ccataaaaat acatatttta aatatgaaag tacaattaca aataaattaa
     1681 aacgttaaaa taaaattgtc aaaacgtcag agaggatggt ggagaatgaa tcaatatgta
     1741 aaaaagctga ttgcgtcgat tttgtttgtc ttccctctct tgatggtgac atccgttttt
     1801 gccgatgtag agaaaacaag cgaatcaacg caatcagaga atcaaagttt tgacaagact
     1861 actttttcag aagaatcaat tacttctgaa actacagaat cccaaagtga ggcgcctact
     1921 tctaatacaa ggcaagaaga aaataagaca agtgagacct cagaagtagc tgaacaaacg
     1981 tctgatagca gtgaagaggt acaggttaga gttgtttcag cggatgggta cagcgaagct
     2041 gccacaacag aagaactgac gcagcttctc gcagacgaat cagtagctaa gattcgttta
     2101 actcagcctt tgactttaga cagggaattg gagatcaagc gagatatcgt tatcgatttt
     2161 ggcggacttg ctcataattt cggcacacat cacatctata tcaatgagac acctcagtcg
     2221 atcgagtttc agaattttaa aggaacggca gctcatccag gtgggttgat accaaatatc
     2281 gatggaaatg ccattatcat tgcttatatg tccgattttt ggggaaacag atatcatttt
     2341 acgggagaaa taaaatttac cgggacactt gacttatatg atggttctaa attaggcctg
     2401 atttacgccc ctcgtgcaac tgtcacatta gatggtgttt ccggtgtatt ggatgtccaa
     2461 ccagtgaaag aagggatgaa tgccgctccg ggtaaagctt atttttgtag aagctatcag
     2521 ctaaatgtaa tcaatggtag tcagctttat ggaccatatc taggaaaatt ctatggtttt
     2581 ctagattcag gtgatgagac gggcagtgga aaaacagcac caggactcca tatcttgagt
     2641 ggttcaaagg tctctcttga ctatgatcgg gcagatggcg tcagcgatga aggagaggct
     2701 gtcgatacgc tacccgggaa tgtcacattt aaagtcagtg gctcaggaag tgaattttcg
     2761 gtaaatacaa agatcaatat taccaacgac aacaatcgcg gaattatcca aatgagaggc
     2821 tcaggatcaa gagtcgctgt gtcgaatgat gggaaaatca caatcaatac agcgactact
     2881 ggcggatttc gcctgcaagg agattctagt caaatccatg tatcaagtgg tggacaaatc
     2941 tcggtcaaaa tggctggaga tggcgaccaa cctggaaaca atgggatgcg ttttgtaggg
     3001 aaaggacttt ctttgatcgt agaaggagcc ggatcagtca tcgatattga taagcaaagt
     3061 ggtcgttcta gcgcagttcg ttttgagaac ggtacgcaaa aactaacagt aactaatgga
     3121 ggctcattga aggtaagaaa tgcaggggat ggcaaatctt atgtgaacag actcaatcaa
     3181 ggaaaccaag cgatccaatt ttatgacgct tctggttttc tagaaagtcc agggtcagct
     3241 gattttacgg ttactggaga aaaatcaaat atcgatatac gcgcagattc cggagctacc
     3301 gtagatacaa ctggagatat tgatttgaac tttatggctg gcgaaaaaac ttatttagtc
     3361 atggtcggga aaactgggga agataagact ggcatcatca gtggggatgt gttgaatgta
     3421 gacttagaat ctcctaccta tttcgatttc caaaacaaac ggatcggaag cgagactaat
     3481 ggcataggtg gctgggtatt tcaaggaaat ggcgattctt ctttgaagat cgctcattca
     3541 gaaattgctt tatggcgaaa ggcaggagta acgttcgata actttgaaaa agatccggat
     3601 tatttttctg atatgacaga cttatcgatc aatggaaccg acttaggtaa ttttgtatca
     3661 agccctagtg caggtttaac aaatgaattt gctcagacag gtgccggaat gaaaaattat
     3721 aatcgaatca gtgccaataa ccagcttccg atgattgaat ccttaagagt accaacgaat
     3781 gcagataaaa aaatctatgg gcatgtcgtt gttcccgaag gtgtcgaggc aaagccaaga
     3841 gatgcttgga caggcgaagt agaagtgact ttacgtatta cttatgcaga tacgacgaaa
     3901 tcacctgtag aaattactgc tctcacacaa ggacaaacag atgaaaatga agggacagga
     3961 tataatccgt ggggagaagg cgaacaaggc ggtttgtttg aggttcaagt acccaacgaa
     4021 gaaagactaa caacggggga tcaagtgcaa atagttgctg ctaaaaaagt aagaggcggc
     4081 caagaagtga cagaactgga ctctgccaaa acgacagtcg atgtgattcc tcctgagcct
     4141 gctaaattaa gtaccacatt gatcaatgca gcttctcgct caatcacagg tacaggcgaa
     4201 gcaggagctt tggtttcatt gaaaaaaaat caagctgttt tgtccgaaac aactattgat
     4261 gatacaggta aatttgttct ttctatcc
//
LOCUS       DAZKMB010000164         4138 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000164 DAZKMB010000000
VERSION     DAZKMB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.169"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1357
                     /locus_tag="ACG8V3_002690"
     CDS             56..1357
                     /locus_tag="ACG8V3_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0301153.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1384..1644
                     /locus_tag="ACG8V3_002691"
     CDS             1384..1644
                     /locus_tag="ACG8V3_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0301154.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2643..3878)
                     /locus_tag="ACG8V3_002692"
     CDS             complement(2643..3878)
                     /locus_tag="ACG8V3_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0301155.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1187 a          668 c          864 g         1419 t
ORIGIN      
        1 ctttgcgttg tcatgcaatg aatggttcat attttattga aaaggagtgg ttttagtgga
       61 aaagctgatg aaatgggtcg aagagaaact tgtcccacca atggcaaaaa ttggtacaca
      121 gcgtcattta ttagcaattc gtaatggggt tgtttcaaca ttgtcattga ttttgattgg
      181 gacatttttt atggtcttta tcaatttacc gtttcctgga tggaatgaat ttattgcccc
      241 ttattcggca acgattgttt taccctttag gatgacgatg ggattgatgg ctatttatgc
      301 aacttttgtt atgggttcgg atctagctaa atcatatgga ttagatagtg tgacaggtgg
      361 tatattatct ttaggaacgt tctttatgtt acaagttcct gtaaacgttt taactcctga
      421 agaggcacct cttggctggg tcttacctat gagttctttg ggagcatctg ggatgtttgc
      481 ggggatctta tctatgattt ttgcagtgga agtatatcgt ttctttaaaa atcgaaatat
      541 tactatcaaa atgccagaac aagtgccacc tgctgttgcc cgtagttttg aggcattgat
      601 tccaggtgca gtcattttaa caattacttg gctgattcga agcgtcgcgg gctttgatgt
      661 aaacgctgcc ttattatcgt tattcgagcc tttaaccaat attttaggga ataatttgct
      721 tggtgtttta ctaccaatgt ttttgatcca tttgttgtgg tcatttggta tccatggcat
      781 gagtattgta ggggctgttg ttcgtccaat gtggctgatc atgttggatg agaatgcaaa
      841 ggcattggca gatggtacag ctgccaccca attaccctat atcgctcctg aacagtttta
      901 tcaatggacg gtaacaattg gtggtgcagg agcaacgatc gtggtatctg ttttgtttat
      961 attcttctgt aaatcaaaat tcttgaaaga ggttggtcgt ttttctatta ttcctggtat
     1021 cttcaatatc aatgagccca taatttttgg agctcctatg atgttgaatc cttacatgtt
     1081 catcccgttc aacttagttc ctttagtttt gactattatc agttattttg cagtcaaatt
     1141 agaaatggtc aatagattta ctgttttgcc aacatggaca ttaccagctc caatcggcgg
     1201 ttatctttca gcaggaaatg actggcgtgt tgttatcttg atcatgatca acacattgat
     1261 agcatttatt atctattatc catttgtaaa agcttatgat aaaaagatgc tgtcagatga
     1321 acaaagtcgt ggagaaaacg aagtccagac tgtctaagtt tggtttttag aaggtgggaa
     1381 acaatgtatt ggctgatttt attttttgtt tttatctttt tattaacggc tagtcacctt
     1441 atattgaata tgctagctac atatcatatt caaatcaatc gatggatttg ggcactggca
     1501 tcatttttga tcgtgattct tccaaagatc attgtaccgc atatgaatgt tttgttttca
     1561 tgggggacat atgtactttg tggaattttt gctatcaatt ttatgatcga gcagcataga
     1621 tggtttgtca catcgaagct ttaaatgttc taaaagtggt tgtaacagaa gtggttagct
     1681 tcaagaaata agaagaaatt tacgaaaatt gcttcttaaa tttttgtgaa atgttggctt
     1741 atttttcgtt tggttgaact tattaccgca gatgcaagct tttgaccacc gtttattcgg
     1801 aatatgggac tgtgacaagg cttttgtcac agcccttttt tacataaaga ttattattgt
     1861 tttaactgtt ttggcagaaa gaaggaggaa aaaatggaaa ttttatcagc aatggattta
     1921 gaattagtag atctactacc aaagtgatca agtcaaatat agatttggga gcttacaacc
     1981 atatagtagg ggctgcagta tggtgtatga atggtaatat gtatgatgtg agtgtttatt
     2041 caaatcatga aacgtgtata gaaataattg tgctgggaat agcaatcagt catggtgaac
     2101 gtgactttta gtgtatcgtt gctgacgaca tagaacattc tgacatgatc tactcaatat
     2161 gtggggattg ctggcagttt atataggagt atgcatcgga ttgcgagatc attgtctcaa
     2221 cagaagaggg aggttgatta tcaaagataa agtggtaaat cctacatttg atttagtaat
     2281 tgttgagggg actagcggca caaatctcta caaagcgatg cttctagatc tatatgacac
     2341 attttaagaa aatgtatttg cctaattatt atggtctttc ttttgaagaa acgttgagat
     2401 gatccaacca gagaaaggtg tcaaagaatt tgactaagaa taaatggcag acgctaccta
     2461 tgctcatttg gatagcgtca gatcttttat tttaaggatg attttgtgtt ttttagacac
     2521 cgtaatttaa gtaactctaa cttttcaatc cactatttac tttttttgta ttttcactgt
     2581 ctgggtctcg aatagcgaaa ttactgatga gtctctatta aaaaagcttt ttctacttga
     2641 aactatattg ggtggttgct caggaaggtt tcgatttctt gaattgcttg ttgtggacca
     2701 tttattggaa aaacggcttg cagcgtgcgt gcctgctgac gaatcttggg tgaagaaact
     2761 tcgcgcagag caatctgcag ttttctttca gtcactggtt ttgtgagggc gattgcgttt
     2821 ccgtatttta cacaatagtc aatattacag ctttgctcaa attgcatccc cacaccgata
     2881 aacggtttgc cggattcaca ggcagtttgg acggtccctt ccccgccgtg gataaaagaa
     2941 aaatcaatga attcatgcaa aagatgacta gacagatatt ctgttattaa aagatcttct
     3001 gagtcagatt gaatgtcatc aagcagatag tcaataggtg caataaccgc tagatccggt
     3061 tgtttttgta agaagaacaa aattttgcgt aacatacgcg gattgcctga actacccatt
     3121 gcgaaataga taatacgttt tctttcttgt tgctttcgct gtataaaagt tagcacttct
     3181 tcaggcaagg aagtactgag cttagcaaaa attggaccaa cgattcgata attttccggc
     3241 agctgttctt cttccaaaaa tagtttaggc gttgtgatga gatttagatc agcatccatc
     3301 atttgtaaac ttgtttttgg taaaggaagc atttcttttt gtgcagcgtg tttgaaggca
     3361 ttggggatcc atcgggtatt tactatacgc ttttttgtcc agtgccaaag gggattgctt
     3421 aaagggccga agaaacgtaa ctcgtggggc atcagcgcat gctctgattg aaaaaaaggt
     3481 tcactcaaag catagggttt aacatatact aaaggaatct ttttattgcg ggccgataaa
     3541 aaaattgtca cattcgaacc tgtcacaatc agtcgaggct caaacttagc aatcagactc
     3601 tgttcactag tgatacgaga ggcaaccatt tctttggtaa aagggttttt aagtgtacgc
     3661 aactggtcaa attttatgat ttgttcttct tgttttttcg taaggatcgg cgctagtaaa
     3721 tgatactcaa agccactttc tgtgatcaaa ggagcaaaag ttttagaaaa gccaaagaat
     3781 acacagtaat aacggtcttt catagctttg gcaacctcaa tcattcgtgt tgtctcagct
     3841 aaattgaata ctgcaggagc aaaaagaatt gtcttcatga aatcctcctt attggaatga
     3901 gtctatccct tcgtttctta ctagtatact aaaaaacaca gcgttcgagt agcctatgta
     3961 tattttgaat attaaactgt ctctcgctgt tttcacaact tctaaattta aattttgtaa
     4021 tcaagttgaa actccaattt tcaaaaaaac acgcaacaat tttttgatcg atttatatgt
     4081 tgtgccagat aaatacgaga agttgtgtca ttaaaaataa acatgagaac ataaaaaa
//
LOCUS       DAZKMB010000165         4124 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000165 DAZKMB010000000
VERSION     DAZKMB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.170"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..327
                     /locus_tag="ACG8V3_002693"
                     /pseudo
     CDS             1..327
                     /locus_tag="ACG8V3_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(599..889)
                     /locus_tag="ACG8V3_002694"
     CDS             complement(599..889)
                     /locus_tag="ACG8V3_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301156.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1291..1527
                     /locus_tag="ACG8V3_002695"
     CDS             1291..1527
                     /locus_tag="ACG8V3_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301157.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1534..3486
                     /locus_tag="ACG8V3_002696"
     CDS             1534..3486
                     /locus_tag="ACG8V3_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0301158.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3558..4055)
                     /gene="dfrG"
                     /locus_tag="ACG8V3_002697"
     CDS             complement(3558..4055)
                     /gene="dfrG"
                     /locus_tag="ACG8V3_002697"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0301159.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1407 a          726 c          707 g         1284 t
ORIGIN      
        1 aaaagaaatc tacgatagtc aacaaaaaaa ttttcattat atccagaaaa atttttctta
       61 gttaaggacc tatggattgc agtaatgcag aattcgtcga ataaactgcc aaagacatac
      121 aatcatattg cttttaaaat tgacgaacaa gatatagatt cgtttgtctc aaaaatccaa
      181 atgttaggtt taactgtcga accaggacgt tctcgtgtga aaggcgaggc ttcttcaatt
      241 tacttttacg actatgataa tcacctcttc gagttacata ccggtactct ccaggaacgt
      301 ttaaaatcct acaattccac tacctgagac tagtaatgac tagtctcttt ttatggcctc
      361 tgatcccccc attgagcgaa tactaatagg gcttcgaatt gatctgttca atctttaagc
      421 aatatccctc aaatgagttt ggaaagttta gtgattcatc acaaatactt aaatacatgt
      481 agttatatat agattgaggt tcttgtgagc gtatgctcgt gtgggttcaa atcccatcgt
      541 ctgcatccat ctttccaaaa caagcggcta ggacattttt gtctcagccg cttgttttct
      601 agtttattct ttcatcttgt cggacctttt ttcttgggta tagacttttc tcatattcta
      661 ccacaatttt gggtttccct aacacatgat aggatctcac aacaaattgc tttctattat
      721 tataataacc tgtaacgact aacttcatcc cttcgtctac ttcagctagg aagttcaaac
      781 tatgggaagc aatcaaacaa ttgaccccat ttaaactgaa acggatcaac ggtctggcac
      841 taacttgcaa agtacggatc ttatctactg ttccatattt tttctccata accttcacct
      901 caataccatt atacgaacat acattccctt tgtaaagaag aaactctgtt ttatcttttt
      961 gactttttgt ctattacctt tcttaaaatc atattggcac ttcctgcgaa gatgaaggac
     1021 atgtactaaa aaaaagaggc agcagtatag atttaagtag ttcagttttg gagtacaaaa
     1081 agtgaaaatg taaacctaaa tgagtatcaa attgcaactg aatttgatac tcgtttttta
     1141 ttgttaaatc aacgtttgtt gttaatttac accacaaaat gtaagttaaa atgtatataa
     1201 aaatgcgaac gtgaaataat tattgtagga atgcaaattg gtgtgttaaa atggttacaa
     1261 tttactaagc aaaaaggggt gcattttcct atgcaatttc ttgagccaaa aaataagaac
     1321 gctaagtctg tggattggga aatatcagag caagtaaggg tgatagttaa gcaatacgct
     1381 gaatatgcag agcgtactga aagcgaagca gtggatgaat tcttattgaa cattttagac
     1441 gataagaagt ttattgaatg gattgccaac aagcgaagta ataagcggat tgttgaaaaa
     1501 atgggtatca aagatagagt gggttgatag acaatgacca agcttaaaag attagccacg
     1561 aaggaagatg aaattgtaga tgttaaaatt ccgatttcaa acgaagaatt aaaagatagg
     1621 gctaaacaat atgatttatt aactccaaaa cgatttgcaa caaggtataa caaaatgttg
     1681 ttcttgccaa cgtcttttaa atggaacggt tctgaatatc ccattcaata taattactgc
     1741 atcaatccgt tttgttgtaa ttttgggaaa gaacagcaca aattcaaaga tgtaaaaggg
     1801 aaaccaagtc gatataaaat gactggtagt tccaaagata aaggtcataa aggtatgtac
     1861 tgcaatgaca atcctattgg cagaggtgta tcgcaaaact gtacagtaac ccctttatct
     1921 aattggtctg ttgttgaaga aattaaacgc cttatagaaa tcaatagcat ccaagatgtt
     1981 gagccagatt atcaatttca taaagaaggc tgctcggaag aagaatcaac cccattcaat
     2041 gagccgaagc aattttacaa aagggggaaa agtaggggca aatctcaacg ctatcaatgt
     2101 aaggcgtgta agaagttcac taatgttcta ccaaaaagag aagaaaccac tacttatcac
     2161 caacaaaaga acaccattct tccaatgttc gctaagatgg ttgttggaag ggtttcagtc
     2221 agtcgtactt gtgatatttt aggaattgga gttggaacct actatcataa gctggagtgg
     2281 ctgtataggc gttgtttgga gtttttagaa cgatatgaga cacaacccct tcaaacaaag
     2341 aaatttaacg agatgtggct taatacggac aagatgcact actacctaaa taacgtccgt
     2401 aagaaaggac aaggttcaaa gaaatatacg gggtttgaag accttaatat gcaaacgtat
     2461 atagttgtat ctgctgaggt cttatcaaga tatgtgttcc gttctgatgt tgcctatgat
     2521 tggaatattt caatggatga attaaatgaa gatacaagaa aattcaaaga agaccatctc
     2581 aatactttta gtcgtaaaaa tgataggtta gattggtctt attatccaca agaaccatcc
     2641 gctaacgata gcgaaaatag gaatgcttat cttcacgaat tagggaaaat tactaatagg
     2701 agtagatttg tcgatggttt aaatgttgat gctccttata ctacaactgc tcactattgg
     2761 ttaattaaac aaatggttaa tgccgacgaa tggcgaatga ttagtgatga tgacttctct
     2821 attagaaacg ccttttatag agtattcacg aaagaattaa ggttgagcga tgcacatcat
     2881 tttatttgcc aagttaacaa gacgaaaagc agaaaacaat gcttaaaaga attcggacaa
     2941 gcaaaagcag aactgctcga ttggggagat atcagaggat tcaaaacaaa gtttctacgg
     3001 actttggcat ctcattatct aactgaatta ctaacaagtc atcaattcca cgaagaggct
     3061 attagtaaag atggagaaag atatcgcaaa tatgccgata accctatcaa acatccatta
     3121 gccactaagg ataaaggatt ttattcagtt gattgtagaa cggatttatc tgctctggaa
     3181 ccaaacgaaa tcgcaaagat gctattaaat gttaatgacc attcgaccaa tagctttatt
     3241 caacagataa gacgttatat atcatctctt gaaagaccat taacaacagc tcgtggtgat
     3301 aagaagagct atatttacgc taacttcaat ccgaagtatg ctcaatttgc gattacaatt
     3361 ctaagaactt actacaactt ttgtcgccct tttaaatcag cggataaaaa ggttttgaca
     3421 cctgctcaaa gattagggat tacagataaa caatttgatt ggaaagatat aatttatttc
     3481 aaatgacaaa aagagcttgg agtaatccca agctcttgtg ctttatcttt actgttcttc
     3541 ttcaattaac gcacccgtta actcaataag ttttttcttt catatacatg aaaatagtag
     3601 ttatacggat ttttatcatt ctttatccct ttttgggcaa atacctcatt ccattcctca
     3661 taattcactt ctggaaaaaa agtatctcct tcgaattcat gatgtatttt tgtgatgtac
     3721 attttctcaa cataagggaa aaacaaatta taaatctgtt ctcctccgaa aataaaaatt
     3781 tcttcttcgt ttttacataa ctcaaaaaca tcttctattg aatgaacaat ttcacaacca
     3841 ttaaaggtaa accccttatc tctcgtcaga ataatatttc ttctgtcagg taaggctctt
     3901 ccgattgatt caaggttctt cctacctaat attatcggat gtccctttgt agtattttta
     3961 acatattccc agtccttggg aatcctccaa ggaatgtcat tctctttgcc aatcactcta
     4021 ttcttatcca tcgcagcaat caaagaaact ttcatttata acacagcctt tttaactaat
     4081 ttaccatcta atttcttatt tctatcaaaa aaccttttct catt
//
LOCUS       DAZKMB010000166         4054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000166 DAZKMB010000000
VERSION     DAZKMB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.171"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..1668)
                     /locus_tag="ACG8V3_002698"
     CDS             complement(163..1668)
                     /locus_tag="ACG8V3_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0301160.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1665..2351)
                     /locus_tag="ACG8V3_002699"
     CDS             complement(1665..2351)
                     /locus_tag="ACG8V3_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0301161.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2544..3965)
                     /gene="gndA"
                     /locus_tag="ACG8V3_002700"
     CDS             complement(2544..3965)
                     /gene="gndA"
                     /locus_tag="ACG8V3_002700"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0301162.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1116 a          857 c          692 g         1389 t
ORIGIN      
        1 ttttttcgtt catctaaaag atacttacat ttaattaacg gatgttattc tatttaattg
       61 ttcgctagtt tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt
      121 attctttgtt ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt
      181 tgcgatagga ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat
      241 acgtccttta tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc
      301 cttcgttcta gctcttgcat tatccactcg ataaaaacga ttgaagatct tattaaggtc
      361 ttctttgggt attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga
      421 agaaatagag atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat
      481 gataatgatt aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa
      541 atcatcatct aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg
      601 ataggtgact tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc
      661 tcttgataag tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct
      721 ggcgcttaat gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact
      781 aagatgccct tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa
      841 ttgctcttgt tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc
      901 taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc
      961 tttacgtata gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga
     1021 taaaaagaaa cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt
     1081 caataatagc ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata
     1141 gccgatcttt tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt
     1201 tccttctagt gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt
     1261 ttcatgggtt ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg
     1321 ctgccctagc tcagctataa aggaatcgat cttcattaag tttccttcga ccagtgaagt
     1381 atgatctacg acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac
     1441 taatgtcagt tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc
     1501 tacattgttt ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc
     1561 gaaaattgta aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa
     1621 ggagggccct ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca
     1681 taacctgttc cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg
     1741 cgaagataac gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac
     1801 actttattta gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc
     1861 agcaactcgt attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg
     1921 ttttcttttt cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta
     1981 tcgccttcaa tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata
     2041 gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc
     2101 acagaatctc ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg
     2161 cagacttcta aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca
     2221 ttatttaatg ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct
     2281 tcatgcttca actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga
     2341 atattgctca tttattattt tatcctttct attctactgg ctttttaaaa actctatcat
     2401 tataccgata atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa
     2461 agagctgaag tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc
     2521 atttcctaac agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct
     2581 tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata
     2641 tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc
     2701 ggtacaggta cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat
     2761 tttttcgtga tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg
     2821 tcataggcat ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag
     2881 atttttgcga tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat
     2941 tgagcaaatc cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt
     3001 tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg
     3061 cttgcttgta cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg
     3121 atcaatggaa gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct
     3181 ttattcccag ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct
     3241 ttacgtgtca gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc
     3301 cattctttaa agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag
     3361 tcataagatt cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc
     3421 acataatgtc cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct
     3481 tttgcagaaa tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca
     3541 ggcatgattg aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata
     3601 aagttgatgc cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta
     3661 tttcctccgt caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt
     3721 gcatctgttg ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat
     3781 tctacaaatt cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact
     3841 acagcggttg ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct
     3901 atgttcaagg ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt
     3961 gacatatgaa ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac
     4021 acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAZKMB010000167         3928 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000167 DAZKMB010000000
VERSION     DAZKMB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.172"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            465..1958
                     /locus_tag="ACG8V3_002701"
     CDS             465..1958
                     /locus_tag="ACG8V3_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0301163.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2093..2389
                     /locus_tag="ACG8V3_002702"
     CDS             2093..2389
                     /locus_tag="ACG8V3_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0301164.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2490..3341
                     /locus_tag="ACG8V3_002703"
     CDS             2490..3341
                     /locus_tag="ACG8V3_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301165.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3362..3574
                     /locus_tag="ACG8V3_002704"
     CDS             3362..3574
                     /locus_tag="ACG8V3_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301166.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1392 a          573 c          796 g         1167 t
ORIGIN      
        1 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
       61 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
      121 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
      181 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
      241 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
      301 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
      361 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
      421 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
      481 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
      541 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
      601 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
      661 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
      721 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
      781 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
      841 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
      901 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
      961 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     1021 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     1081 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     1141 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     1201 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     1261 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     1321 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     1381 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     1441 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     1501 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     1561 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     1621 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     1681 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     1741 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     1801 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     1861 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     1921 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     1981 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     2041 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     2101 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     2161 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     2221 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     2281 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     2341 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     2401 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     2461 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     2521 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     2581 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     2641 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     2701 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     2761 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     2821 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     2881 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     2941 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     3001 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     3061 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     3121 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     3181 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     3241 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     3301 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     3361 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     3421 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     3481 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     3541 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     3601 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     3661 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     3721 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     3781 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     3841 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     3901 cgtaagagaa attgatcgaa gatgtgac
//
LOCUS       DAZKMB010000168         3866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000168 DAZKMB010000000
VERSION     DAZKMB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.173"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..469)
                     /locus_tag="ACG8V3_002705"
     CDS             complement(248..469)
                     /locus_tag="ACG8V3_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301167.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="ACG8V3_002706"
     CDS             complement(567..812)
                     /locus_tag="ACG8V3_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301168.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="ACG8V3_002707"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="ACG8V3_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2036..3037)
                     /locus_tag="ACG8V3_002708"
     CDS             complement(2036..3037)
                     /locus_tag="ACG8V3_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFQ0301169.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3196..3462)
                     /locus_tag="ACG8V3_002709"
     CDS             complement(3196..3462)
                     /locus_tag="ACG8V3_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0301170.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3452..3808)
                     /locus_tag="ACG8V3_002710"
     CDS             complement(3452..3808)
                     /locus_tag="ACG8V3_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0301171.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1434 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatgtatct tatgtaaact
     1861 aataagattt gaattttatt ttatacacac tcgataatag atcaatttta tggtcccccg
     1921 catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt ttcaattcaa
     1981 acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt aaaatttaaa
     2041 acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga gctttgtatt
     2101 tctccataat aacagatatc tttttttcat tttttaaaaa atacctttta aatttcttat
     2161 agtcctctga aatcaaaata tagcgtgatg gatactcatc aaaagaagaa tcgtattctt
     2221 cctttaaaat tcccgattta cttaattttt ctagtacatc aaatactcta tcactcaagt
     2281 aactccttaa attgttatca ctttcccatt gaactacttt tctataagtt gaattatcag
     2341 ttgaaaagat agaattttca tccataaacg catatattag cagcaatctt tcatcattgt
     2401 caaattctat tggatcaaaa atatctaagt tgtcatcaaa atctttatca gttccggtaa
     2461 cttttttgtt aattactgaa ggtgtcttgt aagaaataga atctaaatca aagcgttttg
     2521 attctaaatt attttgtgct ttctcaatta aataattcac tttttttgtg agaaaccttc
     2581 tgatattgtc tttgctatat gttccataaa aacccttatc ttctaaagag tccctgtact
     2641 tcttaacttc tatatactct tcagccaaaa cttgtggaac tggttcatct aagaaaaaaa
     2701 cccctacctc gcctttgcta tttttataaa agtaatcaat ttctgctata gttcccgatc
     2761 tataattatc aactggagaa cccaacttat ggccaaaaat cgctaataat atatcactat
     2821 tttctacaat ttgtttatta ataacacttt gcccagagct attatttata taagtaggaa
     2881 tcgcattcgt ttcccatcga attggcatca aaattacttt tgtattatct gtattttcta
     2941 tattccatct atatatggtt tcttctactt cattcctctt tgctgctata tcacctggtg
     3001 atgctataaa tacttttatt agatttatgg ttttcattta ttgtaccttt ctctttatgt
     3061 ctctttaaag taatttttgt ataaaattta ttgactttta cactatagga caacttttat
     3121 ctatttagta caaaaaggcc cactaaacta attgattagt taggagccta ttacaggtat
     3181 attttcaact cgcttttaag caaattctac taccagcttt ttgcctaatg ccctagcgat
     3241 atcatcaagt ttatcgaaag atacgttatc ccctctctca attcgagcaa tagtagattg
     3301 agttgtatgt gctttttgtg caagctccgc ttgagttagg cctgcttcct cccgtgcatg
     3361 ataaagagca accgcagttt cgagttttct tccttcacgg tcatatgctt ctgcaaaatc
     3421 tttgtctttt tgcttttctt caatcaattt tttaatcatc atttgtgaac cactcctctc
     3481 taagttcttt tgcatgttta agttcactta ttggtgtttt ctccgttttt ttagtaaatc
     3541 catgggtaat gacatacctt ccattgacca catggaaata gatagctctt tggatgtttg
     3601 atcctacctt acttcttaat tcgtacaaat tagtgtctaa ttttttcacc catttcaacc
     3661 gttgagctac tagcatccca ttcttttctg tttcttctat tgtacgtaaa agcttcgcac
     3721 tatctttttt tggtaatgtt tccaaccatt ctaaaaattc atcatgacca ttaggtcttt
     3781 taaatgtttc aaaagatatt ttttccatac ccaattatat catatattga ttttatatta
     3841 tagtatataa cataaaaatt cgaatt
//
LOCUS       DAZKMB010000169         3774 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000169 DAZKMB010000000
VERSION     DAZKMB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.174"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(605..1162)
                     /locus_tag="ACG8V3_002711"
     CDS             complement(605..1162)
                     /locus_tag="ACG8V3_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301172.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1341..1952)
                     /locus_tag="ACG8V3_002712"
     CDS             complement(1341..1952)
                     /locus_tag="ACG8V3_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301173.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2065..2322)
                     /locus_tag="ACG8V3_002713"
     CDS             complement(2065..2322)
                     /locus_tag="ACG8V3_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301174.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2919..3197)
                     /locus_tag="ACG8V3_002714"
     CDS             complement(2919..3197)
                     /locus_tag="ACG8V3_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301175.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3231..3482)
                     /locus_tag="ACG8V3_002715"
     CDS             complement(3231..3482)
                     /locus_tag="ACG8V3_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301176.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1131 a          778 c          512 g         1353 t
ORIGIN      
        1 tcttggagtt aaacgcttct gtcccagcct ccttattccg aataaatggt gtccaaaagc
       61 ttgcatctgc cagattatct catttcttat gacaagctaa gcaaattatc tccaccaaat
      121 aatttattca tatttttgac ttgcgcatac taactaattc ttaaattttc atacaaaatt
      181 tctcaatttc ccttaacgag taatatttat gtacggtgat tctcactagt atcccaatca
      241 ctttttaagt tatcccgatt tcaattctac ttcaaaaata gttgctttta tgtatgctgt
      301 tagttgatta gatggtcatt tgttgagcta ggtcgtttat tttaaacaag ttgtttcttt
      361 agcttatacc tatctcgtct accaaacgca agatggcaaa cacaaatgca tacgcatctg
      421 tatttgccac tttgattcgt ataaagctaa caactattaa ctcctctcag ctaatagttg
      481 ctctcttttt tattcagatt catttttgct gatcacagcc tagtatttca atggttttga
      541 acactaaacc aaattgttcc attacaacat tcactggctg atgaaatgga ttagtagatt
      601 tcgattattc aaagcgattt tctattggtg gatcgtgttc ccaactaata agacggattc
      661 caaacctatc gtacagatct tctaaaccac ttataaattt tgcacctatt tcaggtgttt
      721 tattgcgtgg atattctatt gttacgccat taaatggata ccaattagga ttaggctctc
      781 tttttttctg tacatctccc atagaatagt tagcactttc tgggacataa tatattcttc
      841 gaccatcctc taattcatct aataagatat ttcgtgatcg tctatcactt gctaaatgta
      901 gacgccacgt gcgataccat gccatgctac ctgcatctgc agaaaatcca tcaaataatg
      961 caacaattgt atcttctatc acaatccttt ccccatctac ttcatattct aatctaaaat
     1021 caaactcccc tcgggtaatt tctggtcttg gtggcttagg acccaatatt attctactta
     1081 aaaccgcatt cgttcccaga tgaaacacag ctataacaac aataatgatt aatattttgt
     1141 ttctatttga taaatttctc attattcgca catcacctct acattctcac atattagttc
     1201 gtaatactcc tttaatatca aaatatctac tggtcgtaat aatattcgct atgaattcct
     1261 actactcgta taagaaaggc caaatcatca gcttttactt gctgatgatt tggcttgttt
     1321 ctatttatcg taattgtttt ctaatcacca aaattgttaa caatcggttc agatatttcc
     1381 cagttgatta aggtaattcc gtattgtgtc aaaacttctt gcgcttctcc gaaattaagt
     1441 acagttctta tgtatccact ttctcctaat ataaagtcta cctctctttt aaaaccttcc
     1501 tcgagatttc taagggctat atgtggtttt aatgacattg attgtacatt ttcccccata
     1561 taatattgcg cacttcctcg actaaaatag attcctagat cgtccccaaa aggtagatct
     1621 tctctaccac tagctaatct acttctccat cttcgttctc ttttcataga acctgcgctg
     1681 aactcacacc cgttaaactc agctatgaca gtatcttcaa ttatgttaat ctgtccatct
     1741 agttcgtatt caatcctaaa tggaaattct ccataggtga tttccggttc gggtatttta
     1801 ggtgagatta atgcaaagaa aggaaatatt aagcttggca acatcaaaaa aactcctcct
     1861 atgatcacaa ttcccgatgt aatccaaaat ataatttgta caattctcat tgatccattg
     1921 tattccattt tattccccct attactattc atatccgcct atctatatcg atcttccaat
     1981 gcagagataa ttgcttccat accttgattt tttactgagt tacctatgcg agatatcatt
     2041 ctccactaag gtgcatcata ttgtctatct aacatgatta attctccaat aggtgtatac
     2101 tcctccaaaa acaacattta atgcatctcc ctcgacaaca taagctacca tgtttgctgt
     2161 atatgtgttt gagtctaatt cataagcaga taaatttact gtcgctggat taaatataat
     2221 cgcattcgta tttatagcta ctgaatttgc tgcttcagca ccaccttttg aatgtccgat
     2281 cattgtgatt tcaatagtag gatttacgtg gacagccctc accgtttacc ctgaccatta
     2341 tagaaatcgc ttactttttt gaactacgca acaaaggata caatgctcct aacttcactg
     2401 tctccatttt tgtccactaa ggaatataaa aaataggcat taattcattt aattcggtca
     2461 caacacgtta ccttttaaat cattatactg tatctcatac attattatca ataaactatt
     2521 ataaaaatgc aaaaaatgct ttatcaatct attttgtaat tggtcataat atcattgaaa
     2581 tcataccctg tatcgatttt tcctatgaat ctcgtctatt tgtgattgac gaaaaagagg
     2641 caagacagac actggaatgg taattttacc cactattttg ctccaactat ttgtaatcgt
     2701 acccactttt gaaataccga gatgatcatt ttaattgata tgctgattcc caagcaaaac
     2761 cccaagaaat taaatgctct atcgcatcta gcttcttggg ttttgcttat ataaattcca
     2821 acatttaaat tttttatgct gttcggtctc agaaatgccg tttttgctta tatcacaata
     2881 tcaagatgtc aaattattcg tgttgccgat gactttgatt aatttttttc aaattgttta
     2941 ttaagactaa tgctgtcttt ttgcagatac caatgttcga actttttaac attatcatat
     3001 gatcctgcaa gttttaactt cttaccatta gatgttatca aagttataat taaccttgcc
     3061 tttttcccat taggatcttt tattttatga attttctgca ggtaatctct cctaatggca
     3121 gctattttta cctcatcagt gctgataaac cattgatcat ccacatacat actgcctgtt
     3181 atgttgtgtt tttccatttc ttccgagaac gaaaagttta aggctcgttc ttacttccaa
     3241 agttgaatga gtccatggat tggacttagt gtgagaattg agaacaacaa taagaatcca
     3301 tatcctcccg caaacatccc taaggctata ctgtctctcc atccataagt aggattagaa
     3361 gagaaaagag tcaccgtttg taataatatg aaaaggaata atccagcaca aacaccaaag
     3421 ataacaactc caaacattga atttttatac aatgaccatt tatttatagg cttttctttc
     3481 attttacttc cccctactgt tctgttcaca cggaatgaat tcaaagttca taagccctcc
     3541 tctttgaaca tatgaatacg acttgctaac tatactttaa aaaagcttgt tttaagcggt
     3601 tataggcgag ttgtaatctc tattcatctc attagcaaat aacttgccga aaagtctact
     3661 gaaattaaag cagatagtgg aagaattatt tagccaggat agacactatt tgtgaaacac
     3721 atgtctttac ttttgaaatt ctatgatgaa aagccttgga atacagtagc tctt
//
LOCUS       DAZKMB010000170         3752 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000170 DAZKMB010000000
VERSION     DAZKMB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.175"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..347)
                     /locus_tag="ACG8V3_002716"
     CDS             complement(183..347)
                     /locus_tag="ACG8V3_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301177.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            642..1970
                     /locus_tag="ACG8V3_002717"
     CDS             642..1970
                     /locus_tag="ACG8V3_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0301178.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTVVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2172..3005
                     /locus_tag="ACG8V3_002718"
     CDS             2172..3005
                     /locus_tag="ACG8V3_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0301179.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3021..>3752
                     /locus_tag="ACG8V3_002719"
     CDS             3021..>3752
                     /locus_tag="ACG8V3_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0301180.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKN"
BASE COUNT         1246 a          592 c          781 g         1133 t
ORIGIN      
        1 tgactgttaa tcatgatgtc gtaggttcga gtcctactgc cggagtaaat aaagcttatg
       61 caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat gatgaatcaa
      121 gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt ggctactcat
      181 aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc tacaccaatc
      241 aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt attcataagt
      301 atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt attcatctcc
      361 tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat cttattgtat
      421 gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc atagaaatat
      481 tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga agttgtaagt
      541 atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa attcttaaga
      601 gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct attacgcctg
      661 atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag gcattgcagc
      721 gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca catgtggaga
      781 atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa atgcaatcgg
      841 ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg aatcaattcc
      901 atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa tatgaaaagg
      961 caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat agccggaaag
     1021 tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg atcgtttcat
     1081 tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg aatagttcaa
     1141 attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca gtagtgttac
     1201 ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa gaagaaatgc
     1261 tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa gaattcgatt
     1321 ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca ccacaaattt
     1381 tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc aatgcaccaa
     1441 caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat gtcgttggcg
     1501 ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta gcgattgctc
     1561 agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct tctactagaa
     1621 ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat acggatttta
     1681 caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat gcgatggtat
     1741 tgaccgttgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa aatagctggg
     1801 gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg gacgaatata
     1861 cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat gcgttcaatg
     1921 aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag acgagtattt
     1981 caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct ttttatcttt
     2041 aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa ggataaggat
     2101 tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa ttgggagtag
     2161 gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat ggtaaagtga
     2221 ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa catcgtgctt
     2281 attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa gtagacggat
     2341 taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt gaaacaagtg
     2401 aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa cgagatcttg
     2461 ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt cgtaaagata
     2521 actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg acttttttct
     2581 ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga tggaatgtac
     2641 agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt gaaccaaaaa
     2701 agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc tttgaccagc
     2761 aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa caagatattg
     2821 caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat caaactatat
     2881 ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat tatcttttgg
     2941 cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta ctagaaaatt
     3001 tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa gtttcatatt
     3061 atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta gttatcaaaa
     3121 cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat agctttggac
     3181 ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga gtcggtgaag
     3241 taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat cggaacttct
     3301 ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc gtattgatgg
     3361 attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct tttgagagtg
     3421 agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg ggcttcatgt
     3481 tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat aatgcgatgt
     3541 attttaattg gatcattgac gtattaggat atgacttttt gactacgcat gttccagaaa
     3601 aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa agccattttg
     3661 aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg caagaacagc
     3721 tgtattgtga agcaaatatt atatggaaaa at
//
LOCUS       DAZKMB010000171         3602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000171 DAZKMB010000000
VERSION     DAZKMB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.176"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG8V3_002720"
     CDS             <1..186
                     /locus_tag="ACG8V3_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0301181.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACG8V3_002721"
     CDS             329..553
                     /locus_tag="ACG8V3_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301182.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACG8V3_002722"
     CDS             568..1437
                     /locus_tag="ACG8V3_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0301183.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACG8V3_002723"
     CDS             1418..2152
                     /locus_tag="ACG8V3_002723"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0301184.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACG8V3_002724"
     CDS             2185..3093
                     /locus_tag="ACG8V3_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0301185.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACG8V3_002725"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACG8V3_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DAZKMB010000172         3548 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000172 DAZKMB010000000
VERSION     DAZKMB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.177"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..848
                     /locus_tag="ACG8V3_002726"
     CDS             <1..848
                     /locus_tag="ACG8V3_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HFQ0301186.1"
                     /translation="TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEM
                     TDLWGIGSRMKKRLNQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAI
                     PPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTS
                     ILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQL
                     DLFSDPQKQIDEENLDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAG
                     GLKND"
     gene            841..1191
                     /locus_tag="ACG8V3_002727"
     CDS             841..1191
                     /locus_tag="ACG8V3_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301187.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1188..1358
                     /locus_tag="ACG8V3_002728"
     CDS             1188..1358
                     /locus_tag="ACG8V3_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301188.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(2262..2537)
                     /gene="prgO"
                     /locus_tag="ACG8V3_002729"
     CDS             complement(2262..2537)
                     /gene="prgO"
                     /locus_tag="ACG8V3_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0301189.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(2509..3462)
                     /locus_tag="ACG8V3_002730"
     CDS             complement(2509..3462)
                     /locus_tag="ACG8V3_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0301190.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
BASE COUNT         1196 a          597 c          614 g         1141 t
ORIGIN      
        1 ctacagtagg tgtcggagat aatcccttat tagctaaact tgctttagac aatgaagcaa
       61 aacataatga tggattcctt gctgaatgga cgtatcaaaa tgttcctgat aaagtatgga
      121 atattcctga aatgacagac ctttggggta ttggatctcg aatgaaaaaa agactcaacc
      181 aaattggtat catgagtatt aaagacttag ctaattggaa tccatacact attaagagcc
      241 atttaggtgt gattggctta cagctgtatt ttcatgcgaa cgggatcgat cgaacagata
      301 tagcgatccc accagaaccg acaaaggaaa aatcatatgg taattcacaa gtgttaccac
      361 gagattatac caggagaaat gaaatcgaac tggtggtaaa agaaatggcg gaacaagtag
      421 ctattcgtat caggcaacat aactgtaaaa caggttgtgt ccatttatac attggcacgt
      481 cgatcttaga aacgagacca ggtttttcac accagatgaa gatccctata acagataata
      541 caaaggaatt gcagaattac tgtcttttct tgtttgataa gtattatgaa ggacaagaag
      601 taaggcacgt tggaataacc tatagcaagc ttgtgtacac tgattcgctt cagctggatt
      661 tatttagtga tccacaaaag caaattgacg aagaaaattt agataaaatt attgacaaga
      721 ttcggcaaaa atatggtttt acgtcgattg tacatgcttc ttctatgtta gaaggcgctc
      781 gtagtattac tagaagtaca ttggttggtg gccatgcagg cggcgcagga gggcttaaaa
      841 atgattgatt ataatgatcg tggacgttta aagtgggcag gtttttattt atctgatcat
      901 actgaaaaaa ttgatgccga caatgaacaa cgagccaatc aaaatttggc aaaagaacaa
      961 atgacgactg aagaggttgc ggaagtattg aacgctgcag tcataaaaaa tagatcagta
     1021 tgtattcaaa aagaagaacg aaacgctgaa ggatactatc cagaagatat tgttagcaag
     1081 atcttaggta gtgatgagct ggggatctac gttggaaaaa acaaagttca ctacgacgaa
     1141 atacgtcata tcgaatttta ccaggataat aaatggggtc gattagaatg aacattatcc
     1201 agcaatatga gctaaagtat ataacctttg atcagctatc agaagagatt tggggttatg
     1261 gccagcgttt gataaatgag gttggtgttg aacgcttctc tttctatgta gaagctgcag
     1321 caggatacca taaatacgaa tactatattg gctattaaag tgcaataaaa ttatatttct
     1381 tttatgtttt acattttgta tcaattctga taaaattacc tataggtaat taaaaaatat
     1441 aaaaaaacta gttacaacct taggacgttg taactagctt gaagcatact agagctaact
     1501 aaaatagcac tagaaagcaa tgttaaaccc gtccaacaaa cttgatttaa cattgagacc
     1561 agcttattca gctaaatata gttgaataag cttcttttgt tttaaaaaaa caaaaggtgt
     1621 actacgaata acgcaagtaa aagaaatact attacaacca gtaggctaca agtaagtatt
     1681 actaaacatt gcgtagttag aacctttgct ctattgtcta aagccacaaa attagagatc
     1741 agctttatga tgctaagagc gagctgcatc catgcggaca taggttcttt tgacactatt
     1801 aattctcctt tttaggataa gggaaattgc tagtagtttt ttcattagca ccatccccct
     1861 tcatttgatt gagtctagtg ctgctgtgat agtttgctgt ttaatatgct tacattctta
     1921 ttataacata aatgaaacaa acacatggtt agaggtcgta tcaatcaaaa tagtgtctca
     1981 caaacgattt attacttact cctgctgact aacggactgt tcttcagtaa ctgtaactat
     2041 atgtttgtta aaaccaccat tacctgtaga taaaaaccaa tatatttact atactcacta
     2101 tattaaatat atttagtata gtaaaaacta gaaacaataa aatttgtgaa tgttgtatac
     2161 ccccaaacaa tacacatatt ttgaaggatt aggattaata aagctaaaaa tatagtaagt
     2221 atactaaata tatttactat atctagtata cttactatat tttaatcttt tgatcgtcta
     2281 ttatatataa tccgttcctc gtcgtttaaa gtatcaacat aggcgtccat tgcaagtttg
     2341 actatttcat acataggaat atcttttgca taactaagat cccgaatggt ttttagtacg
     2401 ggaggatcta cttgaactgg ttttctttct ttcgcagtgt actttcgttc ccctaactcg
     2461 acattaatat catctttaat tgaaatatct tcagcaccaa ttttcttctt atctttgttc
     2521 gttggtaata atgccattta gtttaatctc ctttccaaga ggtaggatat gattggccat
     2581 tgagtcagca tatttaggtg tataatcaaa tccttcaata tcaccatgtt gttcatagta
     2641 ttcaattctt tcttctaatt ctgtaaagat gtctgcaaaa atagcccacc atcttcgatc
     2701 tacataatca tttaattgga taccattctc accatacatt tctaaccgat ccgagccttt
     2761 aacaatgctt ttaaaaatgt ttgattcacc atacttatca attgtttttt gatagtttgc
     2821 gtgttgagag gaacgtcgca ctgagaaaag aacaggcaaa attccaatga tttgaagatg
     2881 agactcttca aaattcgtga caatcggaac taaaacttta ttaataaaat cttctgttcc
     2941 ttccattgcg taccgtttta attcttgcat tgcaataata taatctgcag cagttaagaa
     3001 tgcacgaacc acattatctg ttgatggtgg gcagtcgata aagatataat cgaaattttt
     3061 tctcaattct tctaaaggtt taatgaaggc caatgatctc ttacgacgat caggatattt
     3121 ttcggacaag tattcattaa gttcttcaga tccaggagag ccagccataa tatataaatg
     3181 tggacttact tgagtaatac ctgaaaccca gtcccctttt ttgattgctg cagacatgga
     3241 tcgatcaaag ctagtaattt gatacgttga tccaatgttg gaagtagtat ttctttgttg
     3301 gtcataatca attagcaaca cgtctttttc aaaaagttga ctagaaacaa tcgctaataa
     3361 atcattgtct gtagtcttac caatgccgcc ttttctacct gtaagcatta caacttttcc
     3421 tagtttatct gatgttttaa atgcagtccg taagttttcc atagttccct ccaatttcct
     3481 atactaaata tatttagtat acttcatata cttaatatat tcgatatatc taatatactc
     3541 caaattga
//
LOCUS       DAZKMB010000173         3491 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000173 DAZKMB010000000
VERSION     DAZKMB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.178"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG8V3_002731"
     CDS             complement(<1..2232)
                     /locus_tag="ACG8V3_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0301191.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG8V3_002732"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG8V3_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          984 a          772 c          549 g         1186 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac a
//
LOCUS       DAZKMB010000174         3407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000174 DAZKMB010000000
VERSION     DAZKMB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.179"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..1223
                     /locus_tag="ACG8V3_002733"
     CDS             144..1223
                     /locus_tag="ACG8V3_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0301192.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(1524..1766)
                     /locus_tag="ACG8V3_002734"
     CDS             complement(1524..1766)
                     /locus_tag="ACG8V3_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0301193.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(1798..2355)
                     /locus_tag="ACG8V3_002735"
     CDS             complement(1798..2355)
                     /locus_tag="ACG8V3_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0301194.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(2368..2556)
                     /locus_tag="ACG8V3_002736"
     CDS             complement(2368..2556)
                     /locus_tag="ACG8V3_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0301195.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(2569..3135)
                     /gene="amaP"
                     /locus_tag="ACG8V3_002737"
     CDS             complement(2569..3135)
                     /gene="amaP"
                     /locus_tag="ACG8V3_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0301196.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT          944 a          769 c          687 g         1007 t
ORIGIN      
        1 tttattttta aagatatttt ttcataagga ataaagaggc ggattttata attaacttag
       61 ccatttggaa ataaaaaaac gccacgtgaa ttccatgtta tattgaagtt accacaactc
      121 tcaatagaca aggatgaatt cacatgacgc aaaaccatga taacacagtt tcacgcaagg
      181 gaaaacacct aacctactca gaacgttgcc aaatcgccat cctgaaaaag gaaaactatt
      241 ccaaccgtca ggttgcggat gtcctgggct gcgtgccgca gaccataaac aacgaaatca
      301 aacgcgggac cgttactcag cttaaccgcc agaatcagaa cgggaaggtc tacgactatt
      361 atcatgacgt ctacgacgcc gatgccgggc aggcggccta cgagaagaac cgcttgaact
      421 gcggcagacg gcccttatgg gccgacacgg acgccttcat tgaatgggcg gacgacatga
      481 tgctgttgga aaaatggtcc ccggacgtgg tggtgggctt tgccagaaaa cacgaattgt
      541 tcgacccggc cattatccct gggaccacca ccctctatca gtggatcgac cgtggcatca
      601 tgaaaacgaa gaatatggac ctgctagaaa aactgtcccg caaaccgaaa tcgtcccgtc
      661 atggcaaccg cccgaataaa cgggtgctag ggaaatccat tgaagagcgc ccggaggcgg
      721 ttggtacccg gaaaaccttc ggccattggg aagtcgacac ggtcgtagga aacaagacca
      781 agtcggatgc cgtgctgctg acgttggtgg aacggcagac acgcttcgag gtcattctga
      841 agctggccgg gaaggatgcc gaatcggtca accaagcggt ccgacagctg agagaacggg
      901 ctggtgcgta cttcggccag ctgttcaaga cggttacctc ggacaacggc tcggagttcg
      961 cgggactgca cgatgccttg caggatgtgg caaacatcta cttcagccac ccctatgctt
     1021 cctgggagcg cgggacgagc gaaaaccaac acaagctgat tcgccgtttc atccccaagg
     1081 gcagcccaat cggcaacgtg agcgaaaacc aaatcttgcg gattcagcgt tggatgaacg
     1141 attacccgcg aaagattttg ggctatgcaa cgccgcatga cacgttcgtc caggcattta
     1201 ggcaggaacg gttgacggct tgacacgtgg gcccttgaca acgagcctcc ctcggcatgg
     1261 cttgtgtctg gaaagctggc cggggccagc cagtcgggag actaccatgc ctcagctcat
     1321 tgtcaagggc ccactcgatt agaaaaatta gacggttggt ggtaacgagc aaccgggtca
     1381 ccatgggtgg ctaacttaaa cttgaaattt acgtaaggaa taaagaatac ttcatataaa
     1441 atatataaaa aagaaccgca gcgagtgtcg ctacggttct caaatttgtt cttcatttca
     1501 acataagttt ttcttcaatt tgcttataat ttacgtgcaa agaatgaaac gactaatact
     1561 aaaatgattg caccgatcaa tgatggaatg atagccattc ctgcaattgt tggtccccaa
     1621 gttcctagta aagcttgtcc aattgctgaa cctactaaac cagcaatgat gtttgcgatg
     1681 atccccattg atgatccttt attcgtgatt gctcctgcaa ttgctccaat gataccgccg
     1741 acgattaatg cccaaataaa tcccatttta aattcctcct aactgacttc ttacttctta
     1801 ttgtacacgt ggttcgttat tttctttgac tttttgtgca cctttgccta cagcagattt
     1861 tgctttatct gtttggcgtg aagcaaattg tcctgtggat tttgctgctt ctgttacttt
     1921 gtcttgtact gtttcttgat ctttttggta ttcttcttgt gttttgatgt ctgcaacatt
     1981 cacattcact tcgataacat ctaaatgtgt catattgttt acttcagtac tgatcaattc
     2041 tttgatttgg ttgtaaatat cttcgatgtc tttaccgtat tctacgatca catctagatc
     2101 cacagctact tgtttcttac ctacttctgt attgatacct gctgttacgt tatctgtgtt
     2161 gactaatttt tcagccatat ttgagaaaaa tccgccatct aatgtcaata gaccatctac
     2221 atctttcaat gcataaccga tgattttttg gatgactttg tcttcataag tcaattcacc
     2281 attcatcatg cggttttctc ctttttcatt tgccaaaact gatccatttg ttgtattcat
     2341 gtttttgttt tccatctttg tttcctccta tgatttaaaa aattgatcaa ctattcctga
     2401 ctgtttcaaa taaaaaccga ttcctgcgcc aattgctgtc atgattatga tcaacaatgt
     2461 cttgaaaaat ccaatcgtta accataaaac agctagcagt agtccaagac ccccaaagat
     2521 aatagggagc ttgtatgctg aaaatagttc tttcattatt tggccctcct attccactcg
     2581 agagtgcttg tagtctcttg tatcttcttg ttcttcataa ccgctgtatg tgactgccac
     2641 tttgaccgtt tcttttgttc cgagtacttt agttacttct tcttcgatat cttgcatcaa
     2701 tacacctgtt ttccctacta atgaagaagt tcgtttcaag tctccgttga catggatatg
     2761 gattcgattt ttcgttgaac gtatccgtac tttaggtgaa tgaatgaact cttcttcgtg
     2821 taaatgggaa cgtaccaacc cttcgatcgc ttttttgtcg attgttagtt tgccatcttc
     2881 ccgtttcata acgaaagttc ctcttgactt tggatagaaa agaacgacta gcaagatgat
     2941 aatagcaaga acggcaaata ctacagctgc ccagaaaata tattgttgca tgtagtaatt
     3001 cgttaaagta aagaagcgaa atcgttccaa ttgttcaggc ataatatacg gcgcattgat
     3061 caacgcaatc gctagaaata ctgaaagtag cagtaaactc atgataacac cgattatttt
     3121 taacccctta ttcattttcc acctcctata aacttatttt taccctgtaa ataactctac
     3181 cgacaatcac agtatagata gcgaacagtt agcatagcat cagtaaataa agcgctacca
     3241 aacattgctg gatcataacg gcgttttatt gccagtctga tactgaaata acaattgctt
     3301 acttattctc tatttacttt tgattgctgg ctatacttaa aggatattat tcaagattaa
     3361 aaattgcaaa gattatgctc agttattatt acaagattct atttatt
//
LOCUS       DAZKMB010000175         3300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000175 DAZKMB010000000
VERSION     DAZKMB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.180"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..736
                     /locus_tag="ACG8V3_002738"
     CDS             <1..736
                     /locus_tag="ACG8V3_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0301197.1"
                     /translation="NAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKD
                     SFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGV
                     KEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKL
                     DQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKI
                     TTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            726..1466
                     /locus_tag="ACG8V3_002739"
     CDS             726..1466
                     /locus_tag="ACG8V3_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0301198.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1463..>3300
                     /locus_tag="ACG8V3_002740"
     CDS             1463..>3300
                     /locus_tag="ACG8V3_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0301199.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1240 a          402 c          636 g         1022 t
ORIGIN      
        1 taatgccatt actacttgga ataatattaa acagtttttc aaagatactt ggaaaaatat
       61 taaagatacg gcatacaata cgtggatttc tataaaaaat accatgatta atacatggaa
      121 taacattaag gactctttct ggaatattgt tactggaatt gttaattctg ctgaaaatgc
      181 atggacaaat ctaaaaaatg gtgtttcaaa tgctattaat cgggtaaaag aaatctttaa
      241 ttcgttaagg gaagtcaatt tatttgaaat tggcaagaac atcattgatg gacttataaa
      301 tggtgtaaaa gaaaagtgga atgctttgaa agatactatc aaaggtattg caggtaatat
      361 caaaaattgg attaaaggag ctttagatat tcattctcca tcaagatgga tgcgtgatat
      421 ggttggtaaa aatattgttc aaggtattat cgttggtatt gataaagagc aaagtaagtt
      481 agatcaaacc atgacaaatt tagtaaaaac accgtctgtt caacctgtag taaatggaat
      541 aagtcaccta ccaattacac aaacaaagca aaatatatct gctagtgaaa caaaagaaat
      601 tcatttacat ttaaatgttt atggagattt acctgattca atggttcgga agattaccac
      661 aaaaattaaa acagaattga caagacaaat gaaacgagat gctggtgcag taggaggaac
      721 attatatgca acttaagaga ggacaatttt ttattaatca acattattct tctgaattta
      781 atgtgtatat tcaaaacagg cctgcttctg tttcagctag tcgagtaatt gaattgagag
      841 agcgagaagg aaatgattct atcattattg ataaaggcta ttacaaaaat gtgactagaa
      901 agattgagtg ttactataaa gcaccttcaa ttgatatggt gcaggagtgg gaagatcgaa
      961 tcactgaatg gttagatatg tcgtcttata gtgattttat tctttactat gatgaacaat
     1021 atatttatca agctgtagta atagaaggac ctgaatttaa aggaacaaga aaaacaggaa
     1081 atattgtccc ttttgaattt acagtgagta ttcgaccatt taaggaaaat tacaatggta
     1141 gatttactat tcgacaaaca gaaacttttg aaatttataa cccagagaag tattcttcaa
     1201 aaccgcttat taaattgagt ggttctggag atgcttcttt ttatattaat aaggataaat
     1261 atgatttgaa atcgttggac agagaattat ttatagattc taaattagaa gaggcttatc
     1321 gaaaattaga tggtaattta gaacatcagg atcaggtcac cttattttta gattttccat
     1381 ttttatatcc tggaaaaaat gaaattaaat ggacgaaaaa tattcattca tttgaaataa
     1441 tgcctaggtg gtggagaaaa gtatgaaacc aagaatatat agtcctactg aaacagattt
     1501 ctcaacgaat ggcttaggaa ttttaaagga ttgtacaaga tgtggaatat atgaagtagc
     1561 taatggaaaa tatgaattag agttggatta tcctttagga actagatttg atgaatattt
     1621 tgaaaatgac tatcaaataa aagcaaagcc aaacgatcaa gaagagtatc atatcttttt
     1681 tattgatgat aaagatatag atactttttt aaatacagta actatttatg ctcagagtcg
     1741 tacaaatcga cttggaagac gggtagtcac tcttgtggaa gtggactcta aaactggtca
     1801 agaagcgata tcaattattg aaactaaaat ggataaaaaa tctgacatac gactttattc
     1861 tgatattacg gctgtttcta gcacaatctt tgaagcgaga aatgttttaa attgtattgc
     1921 tggtgaacaa ggatcattgc ttcaatattg gggcggagaa attaaacgtg aaccatttaa
     1981 gctttctttg ttaaagcgaa gaggtcgcaa taatgttgga acgattcggt atggaaaaga
     2041 tatgtctggt ttgaaggtca aattagattg gacaggtata aaaacaagaa ttattccata
     2101 tgctgatcct caaagtgaag taggtacgac taatcgaatt tatggttcgc cagtagatag
     2161 tgcctatatt aataactatc ctgatgtcta tacagaacat gttcagttta cagaagaaca
     2221 aggagtaaaa gacattaaga gcttaaataa aatagctaaa aattacttta aaacgatcaa
     2281 tcctggctgt gataagccta aagtttctat tacggttgaa tttgataagt tgactgatag
     2341 tgaagaagcg aaagaatttg cgaagattag aaattatggt ctgtttgata catttaaaat
     2401 atatcataaa aaatataata tttatcttga atcaaaggtt agtggactac aatacgattc
     2461 tttatctgaa aaaactttga aattagaagc aggagacatt caagttgctt tttatcagca
     2521 acaagctgta actattcagg ataaattaaa agattatgca acgaataatt atatgagtga
     2581 ttttaatgat tatgtttctt caatgattac aggtcaaggc agtgcaggag gatatgtagt
     2641 tttatggcca aaagaaaaac cttccaacat ctttattatg gataatccag atttagaaaa
     2701 ggcaagagaa gtactaagaa tgaataaaaa tgggattgct ttttctaaga aaggatggcg
     2761 ggggcctttt aattcagctt ggacattaga tagtatattt aatgccaatt ttatacaaac
     2821 tggattaatc aaagcagata tctttcaaaa ttcttttaac aagactggag atgtattgaa
     2881 attagttaat ggactactcc aaatttggaa caacaaaaag aaaataatgg agttaactaa
     2941 aaaaggaatg gaattttgga attctaaggg ttcaattgga acgattggaa caactgattc
     3001 tgttggtaat ccttttcctg gggcttctac tccaacccct attgaagata attctttagt
     3061 tattcgtaca aatggagacg gcaaatatat tttgatttct cctaaagtag gaaaaggatt
     3121 agttttatta ggaaatggta aagcaattta ttttggagac ttagatgtac aaggaaaact
     3181 tacagttaaa ggaaaagaaa taattcctgg acaaggtggt ggtccatcgg gaggtggaga
     3241 atctactggt gggtatccta gtgaattaaa aacagatgca gaaaagagag cttggagaat
//
LOCUS       DAZKMB010000176         3297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000176 DAZKMB010000000
VERSION     DAZKMB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.181"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            693..1025
                     /gene="traE"
                     /locus_tag="ACG8V3_002741"
     CDS             693..1025
                     /gene="traE"
                     /locus_tag="ACG8V3_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0301200.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1644..1985
                     /locus_tag="ACG8V3_002742"
     CDS             1644..1985
                     /locus_tag="ACG8V3_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0301201.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(2222..>3297)
                     /locus_tag="ACG8V3_002743"
     CDS             complement(2222..>3297)
                     /locus_tag="ACG8V3_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0301202.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1067 a          490 c          674 g         1066 t
ORIGIN      
        1 gataaaaatc acaaacgtag ccttttacgt cataagtgtt aaaagaaata attaatttca
       61 aatatagaaa agggaacgta tgtttgcata ctcctttttt ttgagttata ataatacgcg
      121 aagagagata atttcattat ttaaacaagc taaaaatctt gatattactt cttttcaggc
      181 aatacgaata ttattcgctt acattttttg tataaattta attgtctgct atgcttgtga
      241 tgaattgatt ttcgttttgc acttttggat gataaatttg gacaattgaa aatacaaaaa
      301 aaagaaaggc ggtatgaact cggaaggctg gtaaccttcg tcagcgttca tacacggcat
      361 taagatttgg cccttaatgc gttaaccttt cagtgttacg gtattacccg caatggattt
      421 agtataacag atattcaaaa gtgataccac tttttttggc tgtgttaagg ttaaaaatac
      481 gtgatattac gatgaatcta tgcctaagca taatgttata aaaatgtccg tgtggctctt
      541 tgccgtatga cgaacgctga aattagaggg aacgcatgtt tggatgcgtt ccctcttttt
      601 tgtttgtcat gaacgaaaat caattctcta tttttgaaaa ataacaagaa aaacttgtta
      661 aaaatattta aagaaattgg gtggtttatt ttatgtcata tacaggaatt ttgagtctca
      721 aagatatttg tcactacggt aaacggtgta ccgctacaga gaaaatcacg aagaaactat
      781 caactggcca aagtaaaacc gttgtgcagt gtaagaaata tataatccag aaagacaaag
      841 tctcagagga aatgatttat tacattggga aacaaaagca aataatttta aaagatccta
      901 tccccttaaa agagctatat ccaacaatca aacatgttta tgaccaaaac ggtgtactga
      961 ttggtcgaag aaaaaatgga gtcttgcgtt gtaccgcaaa agggatgggg cgtttgatcg
     1021 gctgaattga tcattctcat aacttgtatt ttatcgtttt tcgcttgctt gaaaatattg
     1081 tcgagacttc taaaggaact tagagtcact agaaaaataa ggagtagact tgatctatca
     1141 acaattccct tcggggagtg acggggtagg ttttaacgat tggtaacgta aaaaattggt
     1201 caatcgagta aatgcgcttg tttggatagt ggtgaaatga atttgtgtaa ataataagcg
     1261 cagagtaaac aagagggaat ttagaagtta gacaagttag aatagcgtct agattaatca
     1321 tgagcagcta ctaagggatc tagactttga cgcaaactca tggaataagg agtaggtctt
     1381 ctcgtaatgg ggaggacgca caagaaattg tgtcgtgtgc tgcagtacat gtagatactt
     1441 gcgaggacgt tgattattgg atcaatcgag catttaccgc tgtctactag ggtttctcgt
     1501 ttcggacgcc ataaacgagt cgaaattttt tatgtatttt taagtaaacg aaaaacgata
     1561 aaatatgaac aagtcctgaa taaagtgaaa aaattaatat aaagcataga gaaaatgaaa
     1621 tttacgtaat ggaggaatga caaatgaaaa attatgatcc gaatatacga ttgggcacac
     1681 atactattaa ggtttctttc caacgttggg attacaaagg atttgtgacg tttagaaggg
     1741 gcggaaattg taaaggatta gatgttttag cattagacga ggatgattta tacgatcaaa
     1801 cattgactga taatccaatt ggattcggct tgctacctga agatgatgaa ggtaatgaat
     1861 ggtttaaaat gacgcttatg aatgataatg gagacgaatt atctgttgaa gatacttggt
     1921 catatttgag tgattatatt gtcagctttg aaatcattga atttgtagcg gataaagaag
     1981 aataaaaact ggaggaataa attttacaga taaaggctta gaaggagtca cgacgattaa
     2041 taaaaaggta gtattacctt aggcattgag taaggaattt atttagtcta atggtgataa
     2101 gtcaagatga aatttaagat tttctttatt gagtaatatg ttttgaagcg cacgaaacta
     2161 gacccgatca aaatcaaatt tttttcgggt tgattcaagg atttgcgtca ataacggatt
     2221 gttatttctc tatcacactc cttggtggcg tatagagttg gtgtttacgt agtagcgtat
     2281 ccaccagacg cgtaaatttt cttgcggtta ggacgagtgc acgtttgtgt tgatgctttg
     2341 gaacctcctt atatttactt tgataaaaag ctttgtattc aggaagataa ttttttacag
     2401 agttggcagc ttcaactaag tagtatctaa aatatcgatt tccttgtttt gtcagaggtg
     2461 tattttgcga ctgatagttt ccagattggt tcactttcca acttaatcca gcgtattttg
     2521 ccaattttgt ctgatcttca aatctttcga tttgaccaat ttcagcgatt aaaccggcag
     2581 catagacttt accaacgcct ggaatactgg ttaagcattg atattctgga atgacaacaa
     2641 ttacttgttc gatggcttta tctaattctt tgatattttt ttcaagtgct cgtatttctc
     2701 gtacgagaac acttaaaaca acattaattg attcttgtgc aagagcacct aggcggtaac
     2761 tcatggtaat tgcacgttga attgctttag cgattttttc tggttgcttg aaacggcctt
     2821 ttcctttttc ttgaagtaaa tcacagaaag cttctaaagg aagctcagca agttcatcca
     2881 atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc gtcgattcgt
     2941 ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga aaatgttgct
     3001 ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc tgtaaggcca
     3061 tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag aaatcagcga
     3121 ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta aattgtttca
     3181 cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc tcatcttcat
     3241 gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca atcacaa
//
LOCUS       DAZKMB010000177         3224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000177 DAZKMB010000000
VERSION     DAZKMB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.183"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            178..774
                     /locus_tag="ACG8V3_002744"
     CDS             178..774
                     /locus_tag="ACG8V3_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0301203.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="ACG8V3_002745"
     CDS             898..2697
                     /locus_tag="ACG8V3_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0301204.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="ACG8V3_002746"
     CDS             complement(2975..3187)
                     /locus_tag="ACG8V3_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301205.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          907 a          573 c          590 g         1154 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg agatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagcagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt tttt
//
LOCUS       DAZKMB010000178         3200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000178 DAZKMB010000000
VERSION     DAZKMB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.184"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8V3_002747"
     CDS             301..585
                     /locus_tag="ACG8V3_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301206.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8V3_002748"
     CDS             588..1004
                     /locus_tag="ACG8V3_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301207.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8V3_002749"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8V3_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0301208.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACG8V3_002750"
     CDS             2522..2608
                     /locus_tag="ACG8V3_002750"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0301209.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..>3200)
                     /locus_tag="ACG8V3_002751"
     CDS             complement(2911..>3200)
                     /locus_tag="ACG8V3_002751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301210.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt attttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DAZKMB010000179         3193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000179 DAZKMB010000000
VERSION     DAZKMB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.185"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..170
                     /locus_tag="ACG8V3_002752"
     CDS             <1..170
                     /locus_tag="ACG8V3_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301211.1"
                     /translation="SELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFI
                     LPDGNEIKIGN"
     gene            186..1028
                     /locus_tag="ACG8V3_002753"
     CDS             186..1028
                     /locus_tag="ACG8V3_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301212.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1091..2893
                     /locus_tag="ACG8V3_002754"
     CDS             1091..2893
                     /locus_tag="ACG8V3_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFQ0301213.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(2952..>3193)
                     /locus_tag="ACG8V3_002755"
     CDS             complement(2952..>3193)
                     /locus_tag="ACG8V3_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0301214.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1143 a          444 c          646 g          960 t
ORIGIN      
        1 tttccgaact tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac
       61 gagaaacggt aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg
      121 tgcaggcttt tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat
      181 ttgtattgaa aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag
      241 gtacttccaa accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa
      301 aacaactgag tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag
      361 caacaacata ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta
      421 taagtgaaac aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac
      481 agcataagtt tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg
      541 ttttgaatgc atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa
      601 ttgatgtaac aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt
      661 tggctaaagt aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc
      721 agttgataaa tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca
      781 agtactgtac atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg
      841 gtatcgttaa aaatactctt ccacactata tccaaggact aactacgaat aagattgata
      901 cgtggcgaag aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg
      961 aaaataatat acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga
     1021 ataggtagaa tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga
     1081 ggaaataata gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca
     1141 cgttgattat caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt
     1201 ggaagtggac atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga
     1261 aacaactggc ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa
     1321 agaaggagtt ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc
     1381 ctcagcttcg gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca
     1441 ggaagtttac ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat
     1501 gtgtgcacac aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg
     1561 gtatgatgct aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg
     1621 cattgtaaat cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc
     1681 ccatagagcg gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat
     1741 tattgaggag tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa
     1801 agaacttgaa gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag
     1861 ttggatcagg tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt
     1921 cttaaagttt aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa
     1981 tggaattgat ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg
     2041 agtctatttt cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta
     2101 tagcgattgt tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga
     2161 agcagagcaa agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt
     2221 attcgatgca aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt
     2281 gatatctaga gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat
     2341 acgaaatata gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg
     2401 agaaactgct aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg
     2461 atataacggc tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat
     2521 taaggattac gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca
     2581 cgatgttggt actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca
     2641 agaggcagag cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga
     2701 tgcgattagt gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg
     2761 aacaataata aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc
     2821 aaaaagtatt agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa
     2881 atacaaagat tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt
     2941 gctagttaat tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct
     3001 cgacttaata gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta
     3061 acagaacgtg agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca
     3121 actgtttttc tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt
     3181 ttacgtggcg gaa
//
LOCUS       DAZKMB010000180         3025 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000180 DAZKMB010000000
VERSION     DAZKMB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.186"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..964)
                     /gene="murB"
                     /locus_tag="ACG8V3_002756"
     CDS             complement(29..964)
                     /gene="murB"
                     /locus_tag="ACG8V3_002756"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0301215.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1037..1957)
                     /locus_tag="ACG8V3_002757"
     CDS             complement(1037..1957)
                     /locus_tag="ACG8V3_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0301216.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2070..2822
                     /locus_tag="ACG8V3_002758"
     CDS             2070..2822
                     /locus_tag="ACG8V3_002758"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0301217.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          852 a          608 c          523 g         1042 t
ORIGIN      
        1 ttttacttat tttaagttcc attacttatt aattggctaa acttctgctt gtttctgata
       61 ttgcttgttt ttctccaatg atccgcactt ctgtttctaa agacacatca aatttttctt
      121 tgattacttc ttggatgtgc gcaatcaatt caatataatc tgtcgctgtc gcatgatcga
      181 tattgacgat gaatccagca tgtttttcag agacttgtgc accgccccat ttcaggcctt
      241 gcaatcctgc ttgttggatc agctgacctg tatagtgtcc tactgggcgc ttaaagacac
      301 ttccgcatga agggtattct aatggctgtt ttgattgacg caattcggtc aactcatcca
      361 ttcttgcttt gattgcttca taatcccctg tttgcaaact gaatcgagct tccaatacga
      421 tagcacgtaa ttcttggact ttactgtgac gataagaaaa taccatttcg tcttttgtca
      481 aagttttcaa tgttccatct tcaagcaaaa gatccacttc agcaaaaaca tctttgatct
      541 ctccgccata cgcacctgca ttcatataaa ccgctccacc gacacttccc gggatgccgc
      601 aagcaaattc aaatccagtc aatgattctg ccaaagctgc ataagtcgta tcaatcagtg
      661 aggcacctgc ttccgcaatg accatcgttc ctttgacatg aatctgtttc atttcagtca
      721 gcatgatgac tacaccgcga atcccgccat cttgaacgat cagattgctt gcatttccta
      781 ataccatcca aggtatatca tggattcggc aataatcgat gatttgcttt acttcttctt
      841 gttttttcgg gaatgcaagg acatcagctg gtccacctgt tttcgtaaac gtatagttca
      901 tcaatggttc atcaaacaat atagtaatcg tggctaagct ttcaactatt tcttctttat
      961 tcactgagga ttttcctttc ttttctcaaa cgtcctcatt attctatcac gcttaataca
     1021 atcttgccag tcttgttcaa agttgatgac ggtagtgatc tttaagcact tcctcacata
     1081 attcatgact tcttacaact tctttttgtt taagcaattc tgtttccgat ttacctaaac
     1141 ggatctgaga aaagaaggcc tctaccattg gctcaaaccc gcgtttgact agcgtgtttg
     1201 tccagtcgcc aaattctttc actttttttc catcagcatc ttgaatgaca agctgggtca
     1261 gatcgtttac ttcataagta ccatttttac ttgttacttg atatttttct atattagcgc
     1321 cacttgctaa atccatcgtt aaaatagccg tttgattttg tgtttctaat tgtaaaatgg
     1381 catattccaa aaattccttt gtttctctta tctggtattt tgttctcaaa atgggttcat
     1441 ctaataaata cacagctgta tcaataagat gtaaaaacaa atcataaagg acgaactctg
     1501 tcgtttcttt tgccgctatt cgattttttt gcaggtggat caaccgctta tctgggatac
     1561 gtttcaattc ttctaccata ggagcaaagc gccggttgaa accgatcatc aaaattattt
     1621 ggttcttatc agctaattct tgcaattcac gaacttcatt tagatggaca ctcagtggtt
     1681 tgtcaatata aacatggata ccattttcta gacatttttt ggctaattca taatgtactt
     1741 ttgttgctgc gtggatcata catgcttcga ttttttcttc taaaagctca tcgatcgttt
     1801 catatagatg ttggaacccg tactcttttt gaattctttc ttttgtttct ttatttcttg
     1861 ttgcaaaata aaaatcaagt gttccttgtt ttttcgcata agttggtaaa tatgcttttt
     1921 ctgcgatatt ccctacaccg attacaccaa tcttcatcta ctttcctcct ggatcattta
     1981 gttactttca tcttacacca ttttttacta aaatgaggta caatagtatc gcaaaggtaa
     2041 ggaaattaat tcgaaggagt gtgcttttat tgaaatttat ctcttggaac gtaaacgggc
     2101 ttcgtgccat cgtaaataaa aattttttag aagtttttca tgagttagac gccgattttt
     2161 tctgtctgca ggaaacgaaa ctccaagcag gacaaattga tttagatctt ccaggttatt
     2221 atcaatattg gaattacgcg gaacgaaaag gctattccgg cacagcgatt tttgctaaaa
     2281 aaccagcatt gaatgccact tacggaatgg gtatcgatat ccatgataca gaaggccgtc
     2341 tgattacttt ggaatattcc gatttctttc ttgtaacctg ttacacgcca aattctcaaa
     2401 gcgaattaaa acgtttagat taccgattag aatgggaaga agccttttat aattatttgg
     2461 aaaatctaaa aaaacaaaaa ccagtcatcg tgtgtgggga tctaaatgtc gctcatcaaa
     2521 agattgattt gaaaaactgg aaaacgaatc aaaaaaatgc tggattcact ccagaagaaa
     2581 gagcggctct ttctcgactg ttggataacg ggtttatcga tacattccgt tatttttatc
     2641 caacccagga aggtgtctac tcttggtgga actaccgatt caattcaagg aaaaacaatg
     2701 ctggttggcg gatcgattac tttttgacga gcaaagattt agagccagga ctagctgatg
     2761 caaagatcca tacggacatt atgggcagtg atcattgtcc agtagaatta gatttgaaat
     2821 aaagaggttg tgaaaaagtc gtttagtttc gagtattaag aaaaattttg tgaaaatggc
     2881 tttccacatt ttgcataaat ttggcataat accgagaaac tggcttttga acactgttta
     2941 tcgagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
     3001 ttcctcatat ttttcgtttg gttgg
//
LOCUS       DAZKMB010000181         2990 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000181 DAZKMB010000000
VERSION     DAZKMB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.187"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..628)
                     /locus_tag="ACG8V3_002759"
     CDS             complement(86..628)
                     /locus_tag="ACG8V3_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0301218.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            763..1152
                     /locus_tag="ACG8V3_002760"
     CDS             763..1152
                     /locus_tag="ACG8V3_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301219.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1156..1920
                     /locus_tag="ACG8V3_002761"
     CDS             1156..1920
                     /locus_tag="ACG8V3_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0301220.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2049..>2990
                     /locus_tag="ACG8V3_002762"
     CDS             2049..>2990
                     /locus_tag="ACG8V3_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0301221.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETT"
BASE COUNT          959 a          501 c          615 g          915 t
ORIGIN      
        1 gatgtttttt aatcaaaata ataaataaaa actgtctaac atttaaaatt taatttagat
       61 attagacagt ttttaagttt gtgagttatt ttccttgtcg ttttctcact tttttagtcc
      121 agaaaccacc tgaaatatat tttggctcat aagaaatgat aaaagcacgc tcttcgattt
      181 ctttgatcag gcgatacagt tcacgttcgc tttttctagg agaaagtatt tcgagaacta
      241 gccgctcacc ttccagcccc attgcagaag tctgtgtcac accataaccg tgttctctca
      301 gtatacccgg caaactttta tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga
      361 tgtatcctaa tgccaatttg tcttcaatca aaattccgac actgatacct actgcgtaac
      421 ctaacgcata gacaaataaa ttcagtggat tatctaaacg atctaaaaca agggataagc
      481 cgacgatata aatcgtgatc tcgaccatac tgacaagtgg agcaatcagt cgatagcctt
      541 tcattgtcaa catgaaacgt aacgtgttca gagtgatata agcaaaatta atgaggaaga
      601 tcatccccaa catttttgga tcaatcattt gtaaaactcc tttcatttat ataacaacaa
      661 tatcaaaaaa aatcgtaagt gaagaaaaat tgaaacttta tacatacaaa aaaatgataa
      721 aatgattata atagaattat aaagataggt taggtgatta aaatgaaaac ggtcaatcgt
      781 gtgttttgtg ccagccacgt gctaagtctc cttctcagta tatacttagg tgtctattcg
      841 attcaacagc caattgaggt agagaacatt gtttttcaga tgtcgctgac agatggttta
      901 attttaagcg tttttttctt tttgatactg tttattggta atattttcgg tagcgttgcc
      961 agttttttga atttttctat ttatccactc ctttcgctag cattaggagt ggtgggactt
     1021 tgttctctgt gcttatttcc cgagccattc agtcctgcat ttatattatt tggtacactg
     1081 aacctgttcc agacaactgt tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa
     1141 ataggtgagt aacaattgaa tccaattata ttgaaacgtt ctgtccatca tattccagtg
     1201 ttagaagttg ttctgaaaga attaagagag gaacgtattc ccgttgtcat ctattatcac
     1261 ggatggcaga caagtaagga gttagttttg acgcaaggaa gaaaaattgc gcagcagggc
     1321 attcgtgtga tcctgccaga tgcgatgaat catggggagc gcaaacagcc ggtttcttct
     1381 attccatcct atacattttg ggatagtatc tacggaaatc tgtttgaatt tgacacactt
     1441 atcaagcatt tagaaaaaag aggtcttcta catgatcagc ttgcagttgg cggggtttcg
     1501 atggggggaa tgacaacatg cgccttgttg gctaaacatc ctcatatagt agcgggcgtg
     1561 tgtttgatgg gttcccctgc acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg
     1621 gaatataatt atgtcgtacc aaaagactat tttgatttga tttcatgggt taagagctat
     1681 gatttgtcat tgcagccgga aaagttagct ggacgtcctc tgttcttttg gcatggacaa
     1741 gctgacgaaa aaatccctta tatacaaacg gaacgatttg tagaaaaaaa tcggcataag
     1801 tcttacggaa aagaaattgt ttttcgttcc tcaaaaacac agcgacatat ggtacaaata
     1861 ccactaatgg aagaagcttc cactttttta gcaaaaaatt tgataaaaaa gtataaataa
     1921 ttgaaacgga aaatcactca tgacgataga ggattttccg ttttttattt tgggctataa
     1981 taaactctgt taggaaaatt tggtataatt gatagtaaga atgacaacat gttagaaagg
     2041 aagagagcat gcccacttta gcagatgttg caaaacgtgc caatgtttca aaaatgacgg
     2101 tctctcgagt catcaatcat ccggaacagg tgactgacga attgaaagag ttagttttct
     2161 cagcaatggc tgaactggat taccgtccaa atattgctgc aaaagcactt gtcacgaatc
     2221 ggtcgcagat catcaaatta tttatactgg aagaaatcga cacaactgag ccctattaca
     2281 tgaatttgct gatgggaatc gctaaacgga tcggtaaagc gcattattct ctccaattag
     2341 ttacaaatga tgccttcgac gtcggttctt gtgatggata catcatcaca ggtgtcagaa
     2401 aaaaagactt cgattggatc gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt
     2461 ctgaaggata tgattatgta gacagcgata atgaatatgg tacagcaaaa gcaacgcaat
     2521 atgccttaga aagagattat caaacgatta tttatatcgg tatcgaagtg gacgagcctt
     2581 ttgaaatttc aagggaaact ggttatttat ctgtgatgaa ggaagttgat cttcctagta
     2641 aaatcttgcg gtttccaaat cattcgacag aagtagaaag atatatgaca gaaaactggg
     2701 cagattttac agataaaact tgttttgtat gtagttcaga tcggctagca ctaggagcag
     2761 taagagggat tataaatgct ggaggacact tgcccgaaga atttggtgtg attggatttg
     2821 atggggtatt tttagaccaa atcagttcac ctaaattgac aacgatcaag caagatatta
     2881 tacgtatggg tgaagtatgt ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga
     2941 gtcaaggtta tcgtcgtttt tttcctgaac tagttatcag agagacaacg
//
LOCUS       DAZKMB010000182         2958 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000182 DAZKMB010000000
VERSION     DAZKMB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.188"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..280)
                     /locus_tag="ACG8V3_002763"
     CDS             complement(<1..280)
                     /locus_tag="ACG8V3_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0301222.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            742..2124
                     /locus_tag="ACG8V3_002764"
                     /pseudo
     CDS             742..2124
                     /locus_tag="ACG8V3_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2359..>2958)
                     /locus_tag="ACG8V3_002765"
     CDS             complement(2359..>2958)
                     /locus_tag="ACG8V3_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0301223.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT         1015 a          562 c          518 g          863 t
ORIGIN      
        1 aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt gatttactaa
       61 aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct tctttcggaa
      121 atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa ccttctctca
      181 atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta gccaataact
      241 caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc ttcctatctt
      301 gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa tttaattgta
      361 ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct
      421 tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa
      481 ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt
      541 aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa
      601 aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa
      661 agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat
      721 aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta
      781 tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt
      841 ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac
      901 cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa
      961 tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg
     1021 cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag
     1081 ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg
     1141 aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg
     1201 gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag
     1261 ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa
     1321 gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt
     1381 ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa
     1441 gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt
     1501 gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa
     1561 cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct
     1621 ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag
     1681 acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa
     1741 tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag
     1801 aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt
     1861 tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca
     1921 ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa
     1981 cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat
     2041 tggtataata atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg
     2101 aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt
     2161 gtaaaaaact attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga
     2221 gcaagaactt atctcattca aggcttgatc ttccaagtaa aacaaaaacc agttaagcac
     2281 tctgcctaac tggttatgac taaattttca gttgtatctt tctctaccaa cactaaatgt
     2341 cgaagagcct tttttcgttt atgaatgtaa gaccgcttct cgagccaatg ccaaacaacc
     2401 gagcgttcca gcgttgtctt ctaatgctgg ggtcacgata tatttgctta actcaggtgt
     2461 ttcgacatac ccattgacca attttgcaaa tgcgtcgtgg acgttttgca gcatatggcg
     2521 ttgtttcatc acaccaccgc caaaaatgat cacatctgga gaaaacatca aggttgtatt
     2581 gtaggcacat tgagcaatat aggaggcttc gatttcccaa taaggatcat cttctgcaac
     2641 ctcttgtcct tttttcccta agcgtttttc gatggcgggt ccagcggcca tcccttctaa
     2701 acagttgcca tgaaatggac agctgccagc aaagtcatct tttggatgag ggacgacgag
     2761 catatgcccc atttctggat gactaaaccc ttcgataaag cgcccttctt gtaaggcacc
     2821 gccgccaaca cccgtaccga tcgtatagta aacgacactg ctcaatcctt tgccactgcc
     2881 gaagacatac tcaccatagg cagccgcatt gacatctgtt gtccaagaaa tcgggatcgg
     2941 aaattctttt ttcatggt
//
LOCUS       DAZKMB010000183         2927 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000183 DAZKMB010000000
VERSION     DAZKMB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.189"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..981
                     /locus_tag="ACG8V3_002766"
     CDS             85..981
                     /locus_tag="ACG8V3_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0301224.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1073..1288
                     /locus_tag="ACG8V3_002767"
     CDS             1073..1288
                     /locus_tag="ACG8V3_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0301225.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1306..1578
                     /locus_tag="ACG8V3_002768"
     CDS             1306..1578
                     /locus_tag="ACG8V3_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0301226.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1580..2443
                     /locus_tag="ACG8V3_002769"
     CDS             1580..2443
                     /locus_tag="ACG8V3_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0301227.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1160 a          464 c          508 g          795 t
ORIGIN      
        1 gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa
       61 atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa agaatggggc
      121 gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat tttaaacaat
      181 aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg aaagtccaaa
      241 ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt aatgatcgat
      301 aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt agaattgcca
      361 cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat tgttcatttg
      421 actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc aaaattaact
      481 cgctcatgga cttttgaaaa tgaaagtaga ttgcttgcta ctcttttagc acctttaaaa
      541 agtgactatg atctcattat tattgatact gtaccaacgc caagcgttta tacaaataat
      601 gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga aagtacaaac
      661 aacattcaaa actatatttc ctatttgatt gatttacaag aacaatttaa ccctggacta
      721 gatatgatcg gttttgttcc ttatttagtt gatacggaca gcgcaacgat aaaatcaaac
      781 ctggaagaac tgtacaagca acataaagaa gataacttgg ttttccaaaa tattatcaag
      841 cgaagtaata aagtaagtac ctggtctaaa aatggaatta cagaacacaa aggctatgac
      901 aaaaaagttt tatccatgta tgagaacgta ttttttgaaa tgcttgagcg aattattcaa
      961 ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     1021 atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt gagtgattgt
     1081 gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg caaaaaaaga
     1141 tataatggga gataagacgg ttcgtgttcg tgctgactta caccatatta taaaaattga
     1201 aacagcaaaa aatggcggaa acgtaaaaga agttatggat caagccttag aagaatatat
     1261 acggaaatat ttacctgata aactttaaaa aggagtgaaa atgaaatggc agttacgtat
     1321 gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta taatcgagta
     1381 ttaaactatg ttttgaacca tgaactagat actaaaaata ctcgtttact agaagtgaat
     1441 cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc atttgaagaa
     1501 ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca aattttgaca
     1561 aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc aatttgaaga
     1621 tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg aatcgccaac
     1681 cgctttttta cttggtgggc aaccaggatc aggtaaaact agtttgcgat cggcaatttc
     1741 cgaagagaca caaggaaatg ttgttatcat tgataatgat acgttcaaac aacagcaccc
     1801 taattttgat gaactagtga aactttatga aaaagacgta gtaaaatacg ttacccctta
     1861 ttctaatcgc atgacagaag cgatcataag ccgtttgaga gataaagggt ataatttagt
     1921 gattgaaggt acaggacgaa caacagacgt tcctattcaa accgcaacaa tgcttcaagc
     1981 caaagattat gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact catatttagg
     2041 aacaattgaa cggtatgaaa ctatgtatgc agatgatcca atgacagcca gggcaacacc
     2101 aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa cccttcataa
     2161 aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac tctattcaag
     2221 cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa atcgtaaagt
     2281 atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg ttcaaaatca
     2341 acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct tgcagccacc
     2401 tacaccacca atacccaaaa cacctaaact tccagggatt taaaccttaa aaaagtaaaa
     2461 gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc tcatttttta
     2521 aaagctgatc gagaaataaa gcacacaact gtattctttc ttttttcagt tggtacattt
     2581 tgcagtctgt aacgtgccat ttactgtttg ttaccttaac atatccagtc gaaaacacaa
     2641 gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt gcgttctcgt
     2701 cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa aaagaaagga
     2761 gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt cacttcctca
     2821 aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaagaaaa gaggaaaaga
     2881 aaatggaaaa aacaaaaaaa ttacaattag aagattttac agaaaat
//
LOCUS       DAZKMB010000184         2920 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000184 DAZKMB010000000
VERSION     DAZKMB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.190"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..377
                     /locus_tag="ACG8V3_002770"
     CDS             <1..377
                     /locus_tag="ACG8V3_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301228.1"
                     /translation="EYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKTEFPND
                     YQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGRREKLPEWAI
                     DQEAYLKKKALERANRQSKAPF"
     gene            389..658
                     /locus_tag="ACG8V3_002771"
     CDS             389..658
                     /locus_tag="ACG8V3_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301229.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            664..828
                     /locus_tag="ACG8V3_002772"
     CDS             664..828
                     /locus_tag="ACG8V3_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301230.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            821..1120
                     /locus_tag="ACG8V3_002773"
     CDS             821..1120
                     /locus_tag="ACG8V3_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301231.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            1120..1422
                     /locus_tag="ACG8V3_002774"
     CDS             1120..1422
                     /locus_tag="ACG8V3_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0301232.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            1462..1827
                     /locus_tag="ACG8V3_002775"
     CDS             1462..1827
                     /locus_tag="ACG8V3_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301233.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            2317..2457
                     /locus_tag="ACG8V3_002776"
     CDS             2317..2457
                     /locus_tag="ACG8V3_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301234.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            2454..2636
                     /locus_tag="ACG8V3_002777"
     CDS             2454..2636
                     /locus_tag="ACG8V3_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301235.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            2649..2843
                     /locus_tag="ACG8V3_002778"
     CDS             2649..2843
                     /locus_tag="ACG8V3_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301236.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT         1058 a          458 c          653 g          751 t
ORIGIN      
        1 aagaatatat aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata
       61 aatacggaga gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag
      121 aattccctaa tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg
      181 gtaagactta taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg
      241 aacctaagaa cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat
      301 gggcaatcga ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat
      361 caaaagcacc attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga
      421 aatagcgaat tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact
      481 cgaagaagct ggaattgaag gaattaagaa tgatcgttcg agttggagta aactcagata
      541 ctatttcgca ctctatatcg atggaacgca attaagaaat ttagcatata ccaaattact
      601 attcattgat tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaaac
      661 aagatggacc taaagacatt tacagcacag atcgaattaa tgcatcaaga agctttaaga
      721 caaagtgtgt cgtacgaaga caagtggctc aacacatttc atggcggacg tgagagcgca
      781 cttgatcaag tactcaaatt actgaaagga gaatgtcggg atggataaga aagcggcaat
      841 gcagcgaatt atcgaattga cttattcaga agattggcag gaagataaaa aaatagttgc
      901 agaagtccaa aagctcggta aatcaatgtg gactgaaaag cccaaacgga gaacgccgag
      961 aaagattgca atctggcatg gtgatcgaat tctagtaacg ggtaccgcta aacagttatc
     1021 tgaaattact ggtctgagca aaaatattat ttgggataga gctaggagct tatggattga
     1081 ttcaaaagga agacagttta ggtatgtgga ggagaaataa tggacgaact aatcgcgaaa
     1141 gtagagcagt gggcaaaaga taagggattg gatcaagcgg atttcagcaa gcaaatgtta
     1201 aaaacggtcg aagaaatcgg ggaagttgcc gcttctctag ctagaaaaga tgaacatggt
     1261 ttaagagacg gaattggcga tgtagtagta accttgatca tcttggcaat gcaaaatgat
     1321 atggatttat acgagtgctt ggattttgct tatgacgaaa tcaaaggtcg gactggaaag
     1381 atggtcaatg gtgttttcgt taagtccagt gacctgaaat aattccatta atggaagaaa
     1441 taactgaata ggaggataga catgattgat gcaaaacaag tgcaaaaaca gaaagatgga
     1501 atgttgatgt ttgaagcata tgttctacca tttcttaatc aatttgaagt tttggaatgc
     1561 tctgccagcg gagaagaatt ggagtatgtt gtaattcgtg aaaccaaaga aaacgttcag
     1621 aagctaaacg aatttctttg cactattaac tgttgggata tgatagctcc aggattttta
     1681 tgtccagcta tgggagaatt tttggaatat tgtcgtcttg aagatgcagg cactttggat
     1741 ttagcttatc tggtatacaa ctacctaaac atcaacacag atcatttgtg gtttggcact
     1801 gccgaaagaa aatgggtagt ccgctaacca ccaatatcaa tgaaaaaagg cggtttcctg
     1861 aaagtggaat tgccgccatt gctgtaaggt tctttacgcg actcaaggtc acagaaatat
     1921 aaatgcaaat ctcttttgaa gtgatccaaa gctctcggaa aatggaagtt ttttgtttca
     1981 aagaataaat gagatttgcg tcaagaacct gagagttatt tccaaaaaag cttccaaaga
     2041 accgaatgag ctaaggacat catgtgtaaa cacataatat agctctcctt tcacggctaa
     2101 tcataacatg aaaaatattc gatgtcagta ggaaaaaagg tgaattatga aaagaatatt
     2161 aaattatccc ggcagtaaca gattctgcaa ttaaactccg aagctgatgt atcagtatca
     2221 actgtagaat attatgagcg tggagaaagt caagcagtca caaatgtaaa aatcgataac
     2281 gtcaacggtc ttatctgttt agatgaaggc gatagtatga atgaaattgc tgcagaccat
     2341 gaattatcag aaactgcttt aattgatcaa atcgaattag ctagacaata ctttgttcca
     2401 tatgcagata cctggtccaa gcataagaat gacatcattt ttccagagga acaatgagag
     2461 caactgatcc agtaattatc cttgaggaag ccaaatttat ttggactcac gaagagatag
     2521 agcaagcacg cttgctcttt tctcaaggag ttaagccgag caaagtagct gaaataatgg
     2581 gtcaaaagat tcttgatgtc ggattgcttt tgctccatct agcagaaaaa atttgatttg
     2641 aggtgaaaat gatgattcaa cttgcaggca tccaaacagg gaaaatttat ttttctggag
     2701 aaagcaaaag cgaagccaac caatggctat tgaaaactta cacgaacaat aagaagctaa
     2761 ggaaaaaata tcctgatttg ttgctaaagg atgatcagat tatgccggaa ccgatgattt
     2821 tgacccgtaa ggagggaagt tgaaactaaa aaaccagccg tgtgggctgg agttgctatt
     2881 tctttttaat aatgagtttt tctaaattta attcattctc
//
LOCUS       DAZKMB010000185         2859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000185 DAZKMB010000000
VERSION     DAZKMB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.191"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..2838
                     /locus_tag="ACG8V3_002779"
     CDS             61..2838
                     /locus_tag="ACG8V3_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0301237.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
BASE COUNT          977 a          514 c          559 g          809 t
ORIGIN      
        1 tcaattcttt attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg
       61 gtgaataagt tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc
      121 gatttagaac atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta
      181 aaaagaaaca cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa
      241 atcaattctc gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag
      301 ttacgcacga atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg
      361 caaccaaaag acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc
      421 gaacaaatca aaacagctct ttactatcca gatggcggat taccactttt gattacaggc
      481 gaaagtggca caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat
      541 gatctgctag aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat
      601 cctgaacttt tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa
      661 gaaaataaaa aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta
      721 catcgcttaa caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt
      781 tatcgtatgg gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg
      841 actgaagaga ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc
      901 gaactacctg cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt
      961 tttctcgaag aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca
     1021 cttttaacaa gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt
     1081 tcagcagcaa aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat
     1141 catttgcaaa aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc
     1201 tatattacgc aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag
     1261 cgaatcgtta aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta
     1321 tattcttgcg aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt
     1381 gagaccagta gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag
     1441 acctttcgac aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc
     1501 ttgagttact atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt
     1561 atccaactaa tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac
     1621 tatgtccaac gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg
     1681 gatcggattt tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg
     1741 ccaaaagcga tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta
     1801 aatcgtttat tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca
     1861 cagcagattg cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg
     1921 atcatattag tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta
     1981 tccgtaccgc ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa
     2041 tgcctgcaaa aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag
     2101 ccagaatggc gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc
     2161 ttcactggaa tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg
     2221 gcttgtcagt tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt
     2281 gacccattat tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc
     2341 accaatatcg actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta
     2401 gctgaatggt tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc
     2461 attcgtaatt tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag
     2521 gtgatgggag aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc
     2581 agcaatccca aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc
     2641 cgacaaatgc caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa
     2701 gggttatctg ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc
     2761 cccaattctg agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg
     2821 atagaagaag aattttgaac ataagcaagt tgttgccac
//
LOCUS       DAZKMB010000186         2835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000186 DAZKMB010000000
VERSION     DAZKMB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.192"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..1062
                     /locus_tag="ACG8V3_002780"
     CDS             55..1062
                     /locus_tag="ACG8V3_002780"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFQ0301238.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1074..2078
                     /gene="neuB"
                     /locus_tag="ACG8V3_002781"
     CDS             1074..2078
                     /gene="neuB"
                     /locus_tag="ACG8V3_002781"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFQ0301239.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2075..>2835
                     /gene="neuC"
                     /locus_tag="ACG8V3_002782"
     CDS             2075..>2835
                     /gene="neuC"
                     /locus_tag="ACG8V3_002782"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0301240.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKM"
BASE COUNT         1055 a          363 c          551 g          866 t
ORIGIN      
        1 ttaaagatta agaatcgaat tatagatgac tataattcat aggaggagat ataagtgttt
       61 aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga aattgttcgt
      121 cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga agaaaaacaa
      181 ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat tggtgatatc
      241 agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc
      301 gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg
      361 atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt
      421 atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa gttattgagt
      481 gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat attttcttgt
      541 gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa
      601 ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt tatgatgtca
      661 ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt tggagaaaca
      721 tatgtcttaa aaatgccggc aatcaatatt ttaactttag ctcaatcatt aataaaaata
      781 tataatgata cacatgatga aaaatataga ggtgaaataa aagttgtagg tatcaggcca
      841 ggagaaaaat tatatgaaga gctaatgact acagacgaaa ttgaacgctc atatgaaaat
      901 gataaaatgt atatcattcc accacactat catgctactg acgaactgta caaagattat
      961 gaaaaaatta aaatcactgc atactcttca gatgcaacag aattaatgag tggacaggaa
     1021 tttattagaa tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt atgatgaaat
     1081 cagaagtata tgtaattgca gaaattggtg taaaccataa tggaagtcta gatttagctt
     1141 tagaaagtat tgataaagct aaagaatgtg gagctaacgc ggttaaattt caaacattta
     1201 aaacgaataa gctagtttcc aaaaaggctc caatggcaaa atatcagaag gataatctta
     1261 aagaggacag ttctcaattt gaaatgctaa aaaaattaga attatctcaa tctgatttta
     1321 ttagaataaa agattattgt atacaaaata aaattgattt tttgtctact ccttttgatg
     1381 aagaaagtgc agactttcta aaacaaattg gtgtacaagg atttaaaatt ggctcaggag
     1441 atttaacaaa tttacctttt cttagaaaaa tagatatgta tgggttgcca gttttattat
     1501 ctacaggtat ggctgatatt catgaggtca gtgaggctgt agattgtttc atagatagtc
     1561 cagttactat tttacattgt acttcaaatt atccggctag ttctgaagat attaatttat
     1621 tggcgattaa cacattaaaa gaaatgttcg ggatacccgt tgggtactcc gatcattcat
     1681 tgggatatga tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt gagaaacatt
     1741 ttaccttgga taaggaacta cctggtccag atcataaagc ttctttagat cctcaggaat
     1801 ttgcagattt tgtaaagcat attagaaaca ctgaaatatt tttaggtgat ggtgttaagc
     1861 gagcaatgcc ttctgaactt agtacaaaag aagtatcaag aaaaagccta gtggtcactg
     1921 agaatttaaa tgtgggagat attcttacag agaaatctct tacaataaaa cgtcctggaa
     1981 atggaatagc tcctaagttt ttatatcaat atattggcaa aaaagttaaa agaccaataa
     2041 gtgcagatag tgttctgctt gaggaagatg ttgaatgaaa aaaatactta tagtaagtgg
     2101 aacacgagct gattatggaa tatataaacc tgttgctgag aatttactac aagataattg
     2161 ggatgtatca tttgttgttt ctggaatgca tttatcccat aaatatggat ttactaaaga
     2221 attgatctta gctgacggat ttagaattag tggagaagtt caaagtttgt ttttagagaa
     2281 caataaggga aacatggctc ggtctgtggc attagagata aatggtttca caaatatatt
     2341 tgaacaaact agccctgatt ttgtattatt actaggggat agaggagaaa tgttggcagc
     2401 tgcgatagct tgtttgtatc ttggaatcaa aacagcacat attcacggag gcgaagtgtc
     2461 tggtactgta gatgaatcaa taagacatgc cataacgaaa atagcatctg ttcattttgc
     2521 tgctacggaa aaaagcaaag agcgtttaat aaaaatgggg gaagattctt ggagagtttt
     2581 tcccgtcgga gctcctagaa tagatactat tttaaataat agattaccta gttttagaga
     2641 tataaaagaa aaatatcatc taaaatttga aaagaaaaaa tacgttttgc aagtatttca
     2701 tccagttgta accgaactag aaaatataga aaaacaaatt ttagctttga tcaattcaat
     2761 ggaacaacaa tctcaaccaa ttatttgcat tttacctaat agtgatgctg gtagcgaaat
     2821 tatcagaaaa atgta
//
LOCUS       DAZKMB010000187         2631 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000187 DAZKMB010000000
VERSION     DAZKMB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.193"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..2563
                     /locus_tag="ACG8V3_002783"
     CDS             122..2563
                     /locus_tag="ACG8V3_002783"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0301241.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          886 a          461 c          617 g          667 t
ORIGIN      
        1 gaaatataaa aaagagaata tttaaggatt gttaaaattt aatcttagaa aaaaataact
       61 ggaacaagag ctatttgttt ttacagcaaa gaatggtata tttttgtagg aggtggctta
      121 cttgaaagaa gtcgttatga tagatgctgc aagaacgccg ataggaaagt atcgcggaag
      181 tttaagtcca tttactgcag ttgaattagg cacacttgtg actaaaggat tattagataa
      241 aacaaaactg aaaaaagata aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg
      301 taatggtcaa aatgttgcca gacagattgc tttgaacagc ggtctccctg tagatgtccc
      361 agcaatgacg atcaatgaag tttgcggatc tggtatgaaa gcagtgatct tagcacgcca
      421 gctgatccaa ttaggtgaag cagaacttgt gatagccggc ggaacagaga gtatgtctca
      481 agcacctatg ctgaaacagt atcagtcaga aacaaatgaa tatggtgaac caatttccag
      541 tatggtcaac gacggattga ctgacgcatt ttcaaatgca catatgggat taaccgcaga
      601 gaatgttgca acacaatttt ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc
      661 ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt
      721 caagatttct gatgaagacg tgttatctga ggatgaagca gttcgtggaa atagtacatt
      781 ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa
      841 tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga
      901 aaataataat ctgccttatt tagcaaccat caaagaagta gcggaggtcg gtattgaccc
      961 aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa ttgacagata gatcaggaat
     1021 gaatttatca acaatcgatc tttttgagat caatgaagca tttgcagctt cttctatcgt
     1081 agttagccaa gaactgcagt tagatgaaga aaaggtcaat atatatggag gagcaatcgc
     1141 attaggccat ccaattggtg caagcggtgc acgtatcttg actacattag cttacggatt
     1201 gcttcgtgaa cagaagagat acggtattgc ttcattatgt attggtggag gtttaggctt
     1261 ggcagtcttg ttagaagcga atatggagca aacacataag gatgtgcaaa aaaaaaagtt
     1321 ttatcagctt acgccatctg aacggcgcag tcaattaata gaaaaaaatg tattgacaca
     1381 agaaactgcg ttgatttttc aggaacaaac actttctgaa gagctctccg atcacatgat
     1441 cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa
     1501 tggcaagaaa aaatggattc cgatggcaac agaggaacca tctgtcattg cagctgcaag
     1561 taatggtgca aaaatctgtg gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg
     1621 gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg atcaatgcag ttaatcatcg
     1681 aaaagaagag ctgattcttt gtgcaaatga aagttatcct tcaatcgtga aacgcggggg
     1741 cggtgttcaa gatatctcta ctcgggaatt catggggagt ttccatgcat acttatctat
     1801 tgatttttta gtggatgtaa aagatgcaat gggcgctaac atgatcaact cgattcttga
     1861 gagtgtggca aacaagttac gggagtggtt ccctgaagaa gagattctat tcagtatttt
     1921 aagtaatttt gctacagaat cattggcttc tgcctgttgt gaaatcccat tcgaacgctt
     1981 agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa atccaacagg caggggaata
     2041 tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa gggatcatga atggtataga
     2101 agctgtagtg gcagctacag gaaatgatac acgtgcggtt agcgcttcga tccacgccta
     2161 cgcggcaaga aatggattat accaaggatt aacagactgg cagatcaaag gggataaact
     2221 agttggaaaa ctaacggtgc ctttggctgt tgccacagta ggcggagctt ccaatatcct
     2281 gccaaaagca aaagcatctt tagctatgtt agatattgat tcagccaagg aactagcaca
     2341 agtaattgct gcagtagggt tagctcaaaa cctggctgct ttgcgtgcat tagttacaga
     2401 aggaatccaa aaaggccaca tgggacttca agctcgttca ttagcgatct caattggggc
     2461 tattggtgag gaaatcgagc aagttgcgaa aaaattacgt gaagcagaaa agatgaacca
     2521 gcagacagct atacaaatat tggaaaaaat ccgggaaaaa taaaaaagca atatctttag
     2581 aagaataaag caagatttac agttatttta tttactgcag atcttgcttt t
//
LOCUS       DAZKMB010000188         2503 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000188 DAZKMB010000000
VERSION     DAZKMB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.194"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..288)
                     /locus_tag="ACG8V3_002784"
     CDS             complement(13..288)
                     /locus_tag="ACG8V3_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301242.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            520..1254
                     /locus_tag="ACG8V3_002785"
     CDS             520..1254
                     /locus_tag="ACG8V3_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0301243.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            1293..2435
                     /locus_tag="ACG8V3_002786"
     CDS             1293..2435
                     /locus_tag="ACG8V3_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0301244.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT          961 a          404 c          412 g          726 t
ORIGIN      
        1 ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag attttttaaa
       61 tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat attttctaat
      121 cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat actctagagt
      181 gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct ctttcacttg
      241 ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat ttccccctcc
      301 tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg ctcaaaataa
      361 aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc atcgacatac
      421 acataatacc tcgataaaaa agaggtgtca atatctttgt aaaatttatt tacaacaccc
      481 ctttttcgag gtatactaat tttaggaggt gtggtcaata tgataaaacg tccaccaata
      541 aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag aaagagtaaa
      601 aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat tgacaaaaaa
      661 actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt atcccgaatt
      721 gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga agaatatatt
      781 ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt tccagataaa
      841 aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt agatacatgg
      901 ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa caagacttgt
      961 aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga agaattattc
     1021 atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag cagtatagaa
     1081 gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga catgtcttat
     1141 cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca aacgatttca
     1201 tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat ctagtggtaa
     1261 gtactccttg taaactaaga ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga
     1321 aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca cgaactggtg
     1381 aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa aaagattatg
     1441 acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat atgacatttg
     1501 aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct tcaacaatcg
     1561 caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag cttaaactat
     1621 cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt aaatatgaaa
     1681 gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt gcaatgcaat
     1741 atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa
     1801 atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt aaagattttg
     1861 gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg agaaagagtg
     1921 aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta catgtcaata
     1981 aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa acaagagctt
     2041 ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac tggaaattac
     2101 agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag caacatgttt
     2161 ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg ttaagatata
     2221 ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat acacatgcta
     2281 gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta gggcatgaaa
     2341 atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct aagaaaacag
     2401 gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa tctggtcatt
     2461 tctagtatac aggcaaaaca aaaaagctag aacccttata tat
//
LOCUS       DAZKMB010000189         2458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000189 DAZKMB010000000
VERSION     DAZKMB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.195"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1149
                     /locus_tag="ACG8V3_002787"
                     /pseudo
     CDS             5..1149
                     /locus_tag="ACG8V3_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="ACG8V3_002788"
     CDS             1320..1778
                     /locus_tag="ACG8V3_002788"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0301245.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="ACG8V3_002789"
     CDS             1937..2407
                     /locus_tag="ACG8V3_002789"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0301246.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          459 g          716 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaata aaaaaagatg aactatttct agttcatctt tttgcctt
//
LOCUS       DAZKMB010000190         2429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000190 DAZKMB010000000
VERSION     DAZKMB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.196"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..2282
                     /locus_tag="ACG8V3_002790"
     CDS             129..2282
                     /locus_tag="ACG8V3_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0301247.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          831 a          426 c          538 g          634 t
ORIGIN      
        1 gtgaaacaaa aagttgattt tatcttgaaa ttaggtaaag aatttcttgc tccgcccatt
       61 ttttcttttt tattaagaag tttttgggta caatgaagca tgaatgtaaa taggcggaag
      121 gataggaaat gatgaaagat tttatgaaaa aaatattaaa tacttcttgg ttagaaaaaa
      181 taagaaaagg tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat gttccttttc
      241 gcttaaattt tctttttttc attatattta cgttatttgt tgcgttgatt gctagattgg
      301 gttatttgca gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct acttctacga
      361 ttacggtcca agaaagtact cctcgcggaa tgatctacga ctccacagga aaagctttag
      421 ttacgaataa agcgaatcag gccatcacat atacgagagg aacacaagtt acggctaaag
      481 atttgcttga aatcgcaacg aagctgaact cgctgatcga tgttccggta gatgaaaatc
      541 tgacagatag agataaaaaa gatttttggc tggctgatcc caaacatttg aaagaagcca
      601 caaaaagact ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat agtgcgatgg
      661 tcaataaagt cacagctgat gagattaatt taaacgagga gcaattaaaa attgctacca
      721 tctttaaacg gatgaatagt gcatatgaat taaacactgt ctatatcaaa aactctggtg
      781 ttactgatca agagttagct gttgtggcag aacgcgcttc tgaacttcca ggagtttcga
      841 ctggcacaga ctggacacga gaatataatg cagcaagttc actgaaaagt attcttggca
      901 gtgtgacgac tgaaaagcaa ggacttccag cagatgaagc agagaaatac ctagcaaaag
      961 gttattctcg gaatgaccgt gtaggacaaa gttatttgga aaaacaatat gaagatgtcc
     1021 tgcaaggaac caaaacccaa tatgaagttt cccttgataa tgaaggaaat gtttcgaatc
     1081 aaaaagaaat cttctctggg gaaaaaggtt ctaatttaat gttatctatg aatgcagaat
     1141 tccaatcaaa agtagaagaa attttgaaaa ggaattatca aacattgatc aacaacggca
     1201 aagcacagta ttcgcctggt gcttatgcag tggctatgaa tcctcagaca ggagaagtgc
     1261 tagcaatgac tggtttcagt catgaacaag gttccaaaga aatcaccgag aatgctttag
     1321 gcacgattac tagcgccttt gatccagggt ctgtagtcaa ggctggtact ttgacagcag
     1381 gctgggcaag taacgcgatc agcggaaatc aagttttgat tgatgagccg attcgtctgc
     1441 aaggtgcttc agaaaaaagc tctgtcttta atcgaagcgg acaagttgct cttgatgcag
     1501 tcaaagcatc ggaattgtct tctaatacgt atatgatcaa agtggcgtta aagatgttag
     1561 gactagacta tacacctgga atgggactac ctagtttaga tgaagaagct aaagcttatc
     1621 agcaattgag agactctttc aaagagtttg gtcttggtac aacgactggg atcgatttgc
     1681 cgaatgaatc tcctgggatc tcacgttctg ttgattatat gaagaagttc aatgcggata
     1741 atggcaaaga atggtatgct ccaggtaact ttaccgattt ggcatttgga cagtttgaca
     1801 catatacacc aatccaatta gctcaatatg cttcaacagt cgctaatggc gggaaacgag
     1861 tacagccgca cctagtcaaa gcgatctatg gcaatgacga aaatggaaac ttgggtgaag
     1921 tgaagaaaga aattggcaca acagtagaaa atacggtcaa tatttctgca gaaaatatgg
     1981 cgatcctccg tgaaggattc caccaagttg tccatggtac cgatccttac acaacagcca
     2041 aaccattagc cagtgcgaaa atggaccttt ccgcaaaaac agggactgca gaaacggttg
     2101 cggaaggtca tccagatatt gcgacagtca atagtaatat cgtggcatac ggaccaacag
     2161 acaatccgga aattgccatc agtgttgtct tgccgaactt attagacgaa caagaccaca
     2221 taaacttgac gattgctaaa gaaataatgg acgcgtatta tgatatgttc atggcaaaat
     2281 aaaaaagagg tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt ttgtgaaaat
     2341 agcttctcac atttttgctt aaatttgact tcataccgag aaactggctt ttgaacaccg
     2401 tttactcggc aaaaaaggtt gtgacaaaa
//
LOCUS       DAZKMB010000191         2417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000191 DAZKMB010000000
VERSION     DAZKMB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.197"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..552
                     /locus_tag="ACG8V3_002791"
     CDS             <1..552
                     /locus_tag="ACG8V3_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0301248.1"
                     /translation="IFITLNDNGQSYADNFTIQGLLEYASRDSGYDGVGDLNRITGIS
                     RVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSFGNLHYQYFHHIWLFIEVGILG
                     VSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVFFFVYNTTLRGETTGPLIYMIL
                     AMPEVVKRKEHYYSEKNTKGEIL"
     gene            549..1268
                     /locus_tag="ACG8V3_002792"
     CDS             549..1268
                     /locus_tag="ACG8V3_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFQ0301249.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1283..2368
                     /locus_tag="ACG8V3_002793"
     CDS             1283..2368
                     /locus_tag="ACG8V3_002793"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0301250.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
BASE COUNT          868 a          261 c          484 g          804 t
ORIGIN      
        1 atatttatta cattgaacga taatggacaa agctatgctg ataatttcac aattcaaggt
       61 cttttagaat atgcgtcaag agattctgga tatgatggtg ttggtgattt aaatcgaata
      121 actggtatct ctagagtagt agatgaaata tttaataata attttagatt agtcctaatg
      181 ggagtaggca ttggcaatgc agagtttacc aatttttttg tcagtgagtt ttataaatcg
      241 tttggtaatc ttcattatca gtattttcat catatttggt tatttataga agttggaata
      301 ttaggagttt cgatattcct attttttata ataaagacat ttttcaatgc aaaaagatct
      361 tttatgaagg ataaagatgc aagtcttgtt aaagttattg caattatgtc ggtattcttt
      421 tttgtgtata acacaacact taggggtgaa acaacggggc cactgattta tatgatttta
      481 gccatgcctg aagttgtgaa aaggaaagag cattattata gtgaaaaaaa tacaaaagga
      541 gagatattat gattcctaag aagatacatt actgttggtt cgggggaaat ccacttccga
      601 aaaaagatag aaagtgcata gaaagctgga aaaagtattg ccctgattat gagattattg
      661 aatggaatga atcaaattat gatttaaata agaaccgtta tatggccgat gcatataaag
      721 aacgtaaatg gggttttgtt ccagattatg caagatttga tattatttat aatcatggtg
      781 gattttattt agatacagat gttgagctaa ttaagtcgtt agatatactt cgtgaaaatc
      841 atgcctatat gggatttgaa ggggaaatct gggttaatgg cggtattggt tttggtgcag
      901 aagaaggaca ttctgttatt aaaggtttaa gggatattta cgattcatta agtttttatc
      961 aaaatgatgg tacattgaac ttgaaaccga gtccgtatta tattacagat tatcttgaat
     1021 ctattggatt ggttcgcgat aacagcgaac aaataataaa tggcctgcat atctatccta
     1081 cggattattt tgctgccaag gattatgata ctggaaaaat ttataaaact agtaatacaa
     1141 taagcattca tcaatataat gcatcttgga aaacacctaa acaaaaattt gtttatagat
     1201 taaaatcaac aattggacaa aaaaaattta acaggttact tgcactgaaa aaatttttac
     1261 acttataaag gagaaaagta ctttgaagaa aatttgtttc attagtggtg taatttctcg
     1321 ttctggggga actgaaagag tagggagtat tattgccaat agcttgtctg atttaggtta
     1381 tgatgtcact attttaagct tttggaatag tggagagcct tattttgggt tagataagac
     1441 gattaaggtt gattatatgt taaatcctaa aacggaggga aaattatata gaacttattt
     1501 atatccggtt tggaaattgc acaagtatat atgcaaaaat gaatttgata tagtaattga
     1561 tattgatact ttgttgtctt tgtatactac aagggcaatt aaaggaacaa agtgcaaatt
     1621 gatttcttgg gaacatttta attactggac aatggaaatg ctgaaagaaa agaagagatt
     1681 caaggctaaa aaattgataa aaaaatatgc aaagaggctt gtggttctca cagaagaaga
     1741 tcgtcaaaaa catattgatg aatatagttt atcagaagat tttgttataa ctatgccaaa
     1801 tccgtgtata tctaatatcg agtacgaata tcattatgat aaaaagagat ttcttgcagt
     1861 agggcgatta gtggaagaga aaaattttgc tgacatgata agtgcttgga aaattgtaga
     1921 aaaaaaagta gatgattggg atcttatcat tgtaggaaaa ggtgaattag aaaaagatct
     1981 tcgccagcag ataaaagatt taaatctgca aagagtaact atagcagggc attcagacca
     2041 tgtggaggaa tattataaat ctgcatcttg ttatgtgctt agttctgttt atgaaggatt
     2101 tcctatggtt atattagaag cacagtctta tggattacca gttatttctt atgattgtaa
     2161 aactggtccg agagatttgg tacatcataa ttttaatggg atgttggttg aagataagaa
     2221 tatagaccaa ttagctaaga gcatgataat gtttactaag aatactgatt tagcaatgag
     2281 aatgtcactg aacgcatata ataatgtaca aaaatttaat ttgaaagaaa ttacaaaaca
     2341 atgggttgct ttgattgaaa atgtatagtt ttttgtggtt gtccaaacat gaaaattagc
     2401 gtaattaatc aaagagc
//
LOCUS       DAZKMB010000192         2398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000192 DAZKMB010000000
VERSION     DAZKMB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.198"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG8V3_002794"
     CDS             207..2069
                     /locus_tag="ACG8V3_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0301251.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACG8V3_002795"
     CDS             2069..>2398
                     /locus_tag="ACG8V3_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0301252.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAZKMB010000193         2387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000193 DAZKMB010000000
VERSION     DAZKMB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.199"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            308..562
                     /locus_tag="ACG8V3_002796"
     CDS             308..562
                     /locus_tag="ACG8V3_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0301253.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            563..880
                     /locus_tag="ACG8V3_002797"
     CDS             563..880
                     /locus_tag="ACG8V3_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0301254.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1439..2374
                     /locus_tag="ACG8V3_002798"
     CDS             1439..2374
                     /locus_tag="ACG8V3_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301255.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          855 a          338 c          453 g          741 t
ORIGIN      
        1 aaaaggcaat acgccttagt tgtcggtctt cgagttacca acggtaaccc tcataccgaa
       61 cggcattcat cacccaccta tagaggatgg gcgacttctg cctaattctg ttaaatggat
      121 gttaacggaa gtgtatggtg agtagttcac tcgtattttg atatcgtttg tatatgtttt
      181 cagcgttcta agtggcttat tttgtccaat aataatatgg agtggaatcc cttaagaaac
      241 accgttggca attgttgtta taacatgtta taatttattc atcaaaaact aaaaggagtg
      301 tgataacatg ggaaaagaac taaaggttag aaagatcggc aatagcgtgg gagtgatcct
      361 tccaagctct cttggattaa aaagcggaga cattatccaa gccaaacaag aaggcaactt
      421 attcatatta gatactactc aaatagcaaa agaacacgat cgcaaattga ttgaagaaag
      481 tttccaagac tttgagaaag gtttaacagt ttcagaaatt gaaatggtaa aagctttcgg
      541 aaaatacggc tggagtgaat agatgaaaga gtacacgcta gtttattcta aaagttttca
      601 gaccagcctt gaaacgataa tagaagaatg ggaaaatgag cttgctcttt ctccagccag
      661 cataaaaaga tttgtaggtg cgatccagaa ggctttagaa ctaaccaaaa attttccaac
      721 tatgcatgag gaagtttccg aaatctatgg aatggatatc ccaacatatc ggatcgtgat
      781 cggcaaaagc tatgccttat tttatcggat cgaccatgaa aaaaaagaga tacttgtagg
      841 acgtattttc caatctaaac aaatgaaact aggcttttaa caataattag gaaaaagtcg
      901 ctcatcctct acaggtgggt gattaatgcc gctcgatatg gttgttaacc gtcggttact
      961 cgaagattga tatataagac ttagtacctt tttattttgc tccatgttat ggccgaaggt
     1021 ctaaagatac aatcattagt ctagagaaaa attaagaacc gtaggaacta cggggatcgc
     1081 ttggtgaata agagacactg ctattagtga aaaaataaga tatcaagtac gctctattcc
     1141 caaaaatttc gtgactttac tcatgagagg ttcaagtagt atatttttaa tcaaaattga
     1201 cgtaactttt aataaatact acctaaataa ggtagtattt atttttgtgt tttaaaagag
     1261 gaagtccaat atatctatgg gaaacttctt aaatattatg ttggtacatg aaaatataca
     1321 tccctgctag actatttaca acattttact ttgtacgtat tcttgtttta aaaggaatgg
     1381 gataaaatat aggtacaata actatttgat gctaaatata attaatggag gagagatagt
     1441 gaacatactt aatgaaaaaa gatatgtatg gtttgctggt aaaaaatata ttactacttt
     1501 caaaagatta tccgagttaa tgcagcagac tgttaaaagt gctatgcctg aaggatacaa
     1561 gtttaatttt gttcaatctt cagtatctac tgaaagtatc tatttttcaa ttctttctga
     1621 gagtaggctt tttcatttta gaataagttc tcatgtaaag aaaactgatg ttgatatata
     1681 tacgttccgt acaaataatt ataaaaattt aaatgatttg agtagaagta tcagtgaata
     1741 tctcaagggg aatagctatc tcaagattcg attttcacat atattaattt tactaccata
     1801 tttgaatgag aatttcaccc taattcaagt agataagtct ttcattgcct caggtaatca
     1861 aggaaatatg aatattacaa gtaacaaaac gtcacgtgtg ctattggcgt tgatgggtta
     1921 cggaattctt tatagagatg accaaaatgg ccagattcta ataaatcggg ctgggcgttc
     1981 attattaatg aaatatagaa aatttatacc ggaagatttt caaaacttac agttagaaaa
     2041 catgcgttta gaagatttat ttagattagc agaagatgat aagtatagat ttgaagtttt
     2101 gaaaattcaa aagaatgaac tattgaataa gttatatcct gagaatgcga taaaagcaca
     2161 ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta gagggaaaat
     2221 caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg taatgataac
     2281 agatatctat gtagaagatg aagatataat caaatatatg aagcctgtga taaaacttgt
     2341 taataaaaat aaaggaaccc gtggtgctag ttaatttttt cctagag
//
LOCUS       DAZKMB010000194         2387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000194 DAZKMB010000000
VERSION     DAZKMB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.200"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..249)
                     /locus_tag="ACG8V3_002799"
     CDS             complement(4..249)
                     /locus_tag="ACG8V3_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301256.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="ACG8V3_002800"
     CDS             complement(266..1117)
                     /locus_tag="ACG8V3_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0301257.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="ACG8V3_002801"
     CDS             1283..2287
                     /locus_tag="ACG8V3_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0301258.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          762 a          403 c          482 g          740 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtg
//
LOCUS       DAZKMB010000195         2374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000195 DAZKMB010000000
VERSION     DAZKMB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.201"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..336
                     /locus_tag="ACG8V3_002802"
     CDS             <1..336
                     /locus_tag="ACG8V3_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0301259.1"
                     /translation="EYLTILKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSK
                     NIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDI
                     KWIQKQIAY"
     gene            309..986
                     /locus_tag="ACG8V3_002803"
     CDS             309..986
                     /locus_tag="ACG8V3_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0301260.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            937..2118
                     /locus_tag="ACG8V3_002804"
     CDS             937..2118
                     /locus_tag="ACG8V3_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0301261.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2131..>2374
                     /locus_tag="ACG8V3_002805"
     CDS             2131..>2374
                     /locus_tag="ACG8V3_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0301262.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEA"
BASE COUNT          877 a          311 c          475 g          711 t
ORIGIN      
        1 gaatacctga ctatattaaa agagtgcaaa tttatgatcg gaaattcttc ttctgggata
       61 attgaagctg catcattcaa aataccagta ataaatttag gaaccagaca aaatggacga
      121 gaacaatcta aaaatattat aaatgcaaat ctaaatgaaa atgaaattga aactgctttg
      181 aataaaatac aagatcctca gtttataaaa agcttgaaaa atgttaagaa catctacggt
      241 gacggcaaca gttctagaag aataacaaaa acattgaatc aaatattgaa tgatggggaa
      301 gatattaagt ggattcaaaa acaaatagct tattaaaata tactagtgat ccatacatta
      361 ttattggggc tggcggacac gcaaaagtca ttattgaaac cttaagatta gaaaataagt
      421 taatatatgg attttttgat gataagcaaa ataaaatgca agatctgaaa tatcttggga
      481 aaataaacga attgaatagc tgggcggata gcaaaaaaaa aatctataaa tttattgtag
      541 ctattggtga ctgtgtagta agaaaaaaga ttataaaaaa attgaatatg ccaagtgagc
      601 aatatggaat agctgttcat ccaagtgcta tagtggcaaa agaaatttca attggttatg
      661 gaactgttat ttttgcgaat gctgtaatta acccagatgc tgtaattgga gagcatgcta
      721 taataaacac aggtagcatt gttgagcatg attgtagaat taataactat gttcatttat
      781 ctccaggtgt atgcttagcg ggtggagtac atgtaggtgt aggaactcaa ataggaattg
      841 gttctcaatg tatccagatg aagaaaattg gatcttggtg tatgataggc gcagggagca
      901 ctattgttaa ggatattccc tcacattcct tagcttatgg caatccagct aaaattaaga
      961 aagaaggaat taattttgaa ttctagaatt ttattatcaa gtcctcatat gggaggaaca
     1021 gaagaaaaat atgttgctaa ggcttttaaa gataattgga tagcgccatt aggtgaaaat
     1081 gtggataaat ttgaagaaag tattacaaaa tatactggtt ctaagggcgg agcttctgcg
     1141 ttaagttcag gtacagctgc catacatctt gccttaagac tgcttaatgt tggaaaaaac
     1201 gatattgtct ttgctccttc atttactttt atagctacag taaatcctat tttatatcaa
     1261 ggggctgtac cagtgttcat tgactcagaa cctaatacat ggaatatgag ccctgttgca
     1321 ctagagagcg cattagaaga agctgaaaga aacaaccaat tacctaaggc aatcatcgta
     1381 gtgaatttat atggtcaaat ggctgattat gaacgaattt tagctattgc aaaccaattt
     1441 aatgttccag tgatagaaga tgcagcggaa tcgttaggtt ctacttataa gggaatttcg
     1501 tcaggcttat ttgggacaat tgggatctat tcatttaacg gtaataagat catcacaacc
     1561 tcaggtggag gagctctagt gtctagtgat aaaaaatatg ttgagcattc aagattttta
     1621 gcaacacaag caagagatca agcaccgtat tatcaacata gcgaaatagg ttataattac
     1681 cgtctaagca atatttctgc cggtatcgga cgtggacaaa tggaagtatt gaatgataga
     1741 gtagctaaac gaagagaaat tttcaatcga tataaggaag ctttttctgg aattgataga
     1801 attgaaatga tgccagaagt ggatggcatg gtgccaaatc gttggttgtc tactttaacg
     1861 atcaatgatt ataattctat tgatcaagta gttaacgtaa taaataaact aaatgaaaaa
     1921 aacattgaaa caagagcttt gtggaaacca ctacatttgc aaccagtatt tcaaggggta
     1981 aaaatgtatt accatgataa taatcttagt gtgtcagaaa agttatttaa tagcggctta
     2041 tgcttacctt ctggaagtaa tatgacagat agtgaacaag aatttgttat tgatgagttt
     2101 aaaaaaatat tcaagtaggt gtgatatttt atgaacatag gaattattgg tttaggttcc
     2161 atggggaaaa gaagacttag actcattcaa gaacacttta atgatatttt actatgtggt
     2221 gttgatacta atgaggcgag acgaaaggaa gttgaaaagg aatttggaat accaacttat
     2281 aatagtctaa cagaggcaaa aaatttgttt gaaataaatt cagtttttgt ttgtacatcc
     2341 cctataaccc atgaatttat tataaaagag gctt
//
LOCUS       DAZKMB010000196         2297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000196 DAZKMB010000000
VERSION     DAZKMB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.202"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG8V3_002806"
     CDS             <1..375
                     /locus_tag="ACG8V3_002806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301263.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG8V3_002807"
     CDS             complement(324..893)
                     /locus_tag="ACG8V3_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0301264.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2257)
                     /locus_tag="ACG8V3_002808"
                     /pseudo
     CDS             complement(967..2257)
                     /locus_tag="ACG8V3_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          638 a          467 c          403 g          789 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttt gatcttcgtt atggtctagt ggaaaaaaat
     1081 gatcggtgat cactagatct gcataccgag gcggcttatt tcgagtacat acatgtatag
     1141 agatccgttt tcgatgtctt agcgttttgt tcagtacccc agcaagtctt cttgcttctt
     1201 ggtgttcata gcgaaaaaaa aagacgattt tcacttcagg agggtggaca tctaaatacc
     1261 ctcgttcctg gagtgttttc agtaaaagca tcccccactc tactgcgggg tgtgcaaatg
     1321 cttgttggat cgctagccga aattgatata gtttatcttt ggaattggga aatttttcta
     1381 atgtttcctg ataataacga acttcttcta tcagccggtc atggatacca attacgtaac
     1441 gaagccattc ttggtaaagt acttttgtca gctttgattt gttttcgctc tcaaattcgc
     1501 tattcagcca aaacagcgaa tcaagctttt cagtcacaat cgtcaatttc cgatagtctt
     1561 gtgtctcttc tatccgtttc caccgctttt cagtaatctg gttacagctc aagcgcaaaa
     1621 ttgctagata atttttttct tttattggca aaatctgctc ttctctaaat agcaattcag
     1681 gaaacaattc aaagaatagt gttcgactgt ctcctaaaat ctcgatttct gccggatcct
     1741 ttctttgctt caataattgg atgaacatct cagaaaagga ctgctctcca tcatctgcaa
     1801 aaaaatgcgg aaaatctttc aaaaagcaaa acagcagata tcttactttt ctttctgaac
     1861 caaagatctg tgctttttta taagaaaaag aaaggtggta ctcgccaaac cactttctca
     1921 atcgctcttg cgtagaacga tcgtacttct gctcgtaagg ttcattcaat agcagatttt
     1981 tcagtaaaca aaacgggggt gattggatca gaaaaacaaa caagagataa ggttcgctat
     2041 atccttgatc ataaactaac tgaacggttt tcttatgaag aatcagctgg atgggacagt
     2101 cgctttccgc caaacgtttc agatgccgat ccactgtttt aacatcgatt ttcagttctt
     2161 cagctaaatc agcttttcgc cagatcaaat ctttcattag attgattagt gaaaataaat
     2221 tctgtgcatt ttttgataat agtttttgcc atctcatcga tcatttctca cctgatttct
     2281 aaacatattg tttgatc
//
LOCUS       DAZKMB010000197         2256 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000197 DAZKMB010000000
VERSION     DAZKMB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.204"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1125
                     /locus_tag="ACG8V3_002809"
     CDS             <1..1125
                     /locus_tag="ACG8V3_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0301265.1"
                     /translation="SSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTE
                     GQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDL
                     YASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQS
                     KFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQY
                     YLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAA
                     TQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQI
                     IGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="ACG8V3_002810"
     CDS             1474..2106
                     /locus_tag="ACG8V3_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0301266.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          839 a          307 c          454 g          656 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggata
//
LOCUS       DAZKMB010000198         2205 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000198 DAZKMB010000000
VERSION     DAZKMB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.205"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8V3_002811"
     CDS             complement(<1..914)
                     /locus_tag="ACG8V3_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0301267.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8V3_002812"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8V3_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0301268.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACG8V3_002813"
     CDS             2006..2176
                     /locus_tag="ACG8V3_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301269.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          747 a          384 c          421 g          653 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtata
//
LOCUS       DAZKMB010000199         2189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000199 DAZKMB010000000
VERSION     DAZKMB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.206"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..356)
                     /locus_tag="ACG8V3_002814"
     CDS             complement(57..356)
                     /locus_tag="ACG8V3_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0301270.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            707..1339
                     /locus_tag="ACG8V3_002815"
     CDS             707..1339
                     /locus_tag="ACG8V3_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0301271.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1462..2100
                     /locus_tag="ACG8V3_002816"
     CDS             1462..2100
                     /locus_tag="ACG8V3_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0301272.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          721 a          352 c          323 g          793 t
ORIGIN      
        1 gcctgcatta tgagattgac tatacttttt aattcattga gatgaataac tcgtttttat
       61 aaatgcccaa aagtaatcgg tgccgaagcc attcctcgac ctaattcttt ttcaaatttt
      121 cctattgttg atatctccat ataaaacggt gatactggtt tagacaattc tcccctgatt
      181 gctaacggac gtaaactacg ttccaaatta tttatgactt taggataata ctgacctttt
      241 tctaagcctg tctttgcgtc tatcaaaagt atacgttcct tttctttgat ctctggattt
      301 aaaacgagat catataattt tagtaccatg ttttctattt cttcactttt agacatcacg
      361 atttcctccc aaaaatatat ttcttcccac tacattctat aaacaaagta tacgctacat
      421 ctagattttt ttaaatcatt actggattgt taaaaccttt caatacactg ttatggcaac
      481 gatttttatt ttaaaaacat tctaaattaa atcagtaaaa atttttattc taagattttt
      541 attagaatta cgcctaattt gtgtctctca gggcaattct aagaacgttt caagagatag
      601 cgacactatc gttatcgaaa aaggcgaaaa cctatctcat tttctgctaa tgtaagttgg
      661 tttagctttt gttaatatga ctttatgaaa atggaggtaa ttaaatatga tttttcctaa
      721 agttaatgaa gcaagcttga ataaaggatc cttttttcat atctatgaaa acgaagattt
      781 tgaactgtat aatatccttt cacaaaaaga ggtcatcgaa aaccaaataa tctgccatcc
      841 aattatttac aaatctattt tcaagtatgc attttttgct tccaatgatt ttcaaagaat
      901 ggagattacc gactgtatat tcgaaaactg tgatttttca ggctgtatat tgacaaaaag
      961 catgttgcat cgcgtgaaat ttgtcaattg taaattaaca ggtactaaat tgatggaaag
     1021 ttatattggg aacacgttat tcgagaattg taaaatggac tatgtaaatt tatctggttc
     1081 aaacataaaa gaaagtacct ttgagcactc tgtcttatca agcgcagatt ttgtagactg
     1141 ttccctcact aaaataatgt tttctactaa tgatttagaa aatgctagct tttttgatac
     1201 tgatttaaaa aatatcgatt taagtagcaa ctcctttgaa atgattgaag tacgctcgga
     1261 acatctaaaa aattgtacgc ttaatcagtt gcaagcactt ggttttgcta gaaaattttt
     1321 acaaataaaa gtagtataat caaaatgaat atataagata aatttttagc aatacaaata
     1381 tccataaaaa aacattgcct attttaactc ttcatgtata ctgactctat cttcaaaaac
     1441 tgaatagtag gagttattat catgaataac attttaggaa aaaaaataga acttaaaaaa
     1501 gggatattgg gcactattgt cgttgtactc ggtttgttag ggttgctatt tgcgccatcc
     1561 attattagca ttttttcttt aactatcact ggatttatag catttacact tgtttttggt
     1621 aaaaaagata cgaaaaaaat gttctcgaaa cctgtcgctc ctgtcaaaaa tatagccaaa
     1681 tattttttta tcaatgtagt aatcagtgcc gcagtctcgg tatttttgca acaaattttg
     1741 aaatggggtt taacaggcaa tccaataaat gaggctttca gcccgttatt atttattatt
     1801 ttgcctatca tgatacttgg agaagaattg ttctcagtgt attttctcgc tattttttct
     1861 agtaagttca gcattcctgt cgctagtttc ttgtcagcaa tcatctttgg atttatccat
     1921 tacagtactt atgataatgg aaatatttta cacactgtag cacacatcct tttgatacaa
     1981 ggggtggcaa gattgttatt caaccaagct gccattaaaa gcaactcgat cattacaagt
     2041 tgggcagttc atgtgatatt tgatttcaca gctatattgc tagtcgtttt attctcctaa
     2101 atttttatag aaataataca tgctctattg ctaagaaagc atactttatt tcctttaaac
     2161 aaaaagacag atgtatcaat ggattaaaa
//
LOCUS       DAZKMB010000200         2044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000200 DAZKMB010000000
VERSION     DAZKMB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.207"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..1056
                     /locus_tag="ACG8V3_002817"
     CDS             148..1056
                     /locus_tag="ACG8V3_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0301273.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1056..1673
                     /locus_tag="ACG8V3_002818"
     CDS             1056..1673
                     /locus_tag="ACG8V3_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301274.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1677..>2044
                     /locus_tag="ACG8V3_002819"
     CDS             1677..>2044
                     /locus_tag="ACG8V3_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301275.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTT"
BASE COUNT          739 a          294 c          438 g          573 t
ORIGIN      
        1 gttatccgag gcttagctaa tggtcgtatg caaacttatg aacaaaacac agagttaggt
       61 atgatttgtg gaaagttaga tcgccagtat tatagtgcaa gtgcaatttc ttccattgtc
      121 ataccaccaa aatagggagg tgatttaatg gcaaagtgga atgtcatatt aagtacaaca
      181 gaaccctata attacgtggg gatgattcaa gttcgacaag gcaataaaaa cactgaagtt
      241 atggaagcta caattgtcga aaatggtctt ccatatgatt tatcagaatg taaggtatat
      301 tttgaatcag ttgttggcgg aaaatatcca gtccaattag aaacaagaat tgtggatgct
      361 aaaaaaggga aaatcaaata tatttttgat aaatattcca tgcagtgttt gcatcgacaa
      421 acagccaatt tcattatatt taaaggagaa gacttgattg gaacaactca agacttctcc
      481 tattttgtca tcaatgctgt ttcaaaaaca gaaggagaaa tgggttctta ttggcaatca
      541 attgaagatt taattgctga tatgacagat tttattaatg aaaataaagg cgattttacg
      601 gactggatga atgaaaggaa agaagaattt gatcgctgga gagaagaaca ggagaatagt
      661 ttccaagatt ggagagaagg acaagaatca gattatttgt catggtttga atcaatcaag
      721 gatattttga agtctatcga tccaggtgga gtaatgttag ccgaattaat ggatgcacgt
      781 gtagatcttc aaggtgtacg ccatgaatcg atttctgaac gtttcctatc cgatctaaat
      841 tatttgtatc aaaaaatgaa agcagcactt ttcacgattg aatacggtga aattgaagtg
      901 actgatattc ttcaagacga tctcttttca gataatcacg aagtcgaaaa aattgaaaca
      961 gtagaatttc caattgaaga aggagctttg attattgcaa ctgttgatga tccaaaacag
     1021 aatgttttca ctcttgagaa agttggggtg atttgatggt taaagtaaag agaatgatgg
     1081 agactgatga atctggtgtt aaacgtcaat ttatgccaat tacacatgtt tctgcagttt
     1141 taggcttaga aaaaatgata tcaggacaat caaaagttct ttcggttaat ggtaaatatg
     1201 gtgcagtcat tttaacgaaa gctgatttag gattagagaa tgcaattact gaactacctt
     1261 atgcaagcga gacaagcgat ggtattctta ctgctgaaat gtttcaaaag atcgtaaatg
     1321 gagaaggtgg aacatatatt ttgccgattg ctactcctga acaattagga ggtataaaag
     1381 ttggggaact cttagaagtt actgaagaag gtgttttatc tgcaactaag caaacagact
     1441 tcaatttctc tgaagaatta aagggaaaat tagaatctct aaaaatatta aaagcaggtg
     1501 caaatatatc aattgcagaa gatggaacaa ttagttctac aggtggtagt gggactggtg
     1561 gtgttaatca gtcatatgta gatcaaaaat tccaagaagc tgtaaatcaa gcagaaaatt
     1621 acacaaacga aagaattcca aattttactt ttgaaaaaat cggggaggta tagaaaatga
     1681 cagatatcgt aaaaataaaa caaagcggtg tacaggttta tccacaaact cattggaatg
     1741 ctatagaagg caaaccaaca acggtgaagg gagataaagg cgatcctggc caagcagcaa
     1801 caattaccat aggaaccgtt tctagtggtt caaccgcttc tgttacgaat gtaggaacat
     1861 catctgcagc tagatttaat tttgttttac caaagggaga taaaggtgat cctggaataa
     1921 atgccacaac tacagctgta gctacaacta cagcaaatgg gttgatgtcg tccacagata
     1981 aaactaagtt ggatggaatt gcagctggag cacagaaaaa tccaggtaat gctacaacta
     2041 cgac
//
LOCUS       DAZKMB010000201         2040 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000201 DAZKMB010000000
VERSION     DAZKMB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.208"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8V3_002820"
     CDS             200..742
                     /locus_tag="ACG8V3_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301276.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="ACG8V3_002821"
     CDS             898..1347
                     /locus_tag="ACG8V3_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0301277.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2040)
                     /locus_tag="ACG8V3_002822"
     CDS             complement(1377..>2040)
                     /locus_tag="ACG8V3_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFQ0301278.1"
                     /translation="RRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQE
                     QPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSL
                     TDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQ
                     YQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGE
                     AA"
BASE COUNT          733 a          322 c          391 g          594 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatccg cggcgtttca tgattcgacc gattcggcgc
//
LOCUS       DAZKMB010000202         2025 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000202 DAZKMB010000000
VERSION     DAZKMB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.209"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..1212
                     /locus_tag="ACG8V3_002823"
     CDS             43..1212
                     /locus_tag="ACG8V3_002823"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0301279.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1479..>2025)
                     /locus_tag="ACG8V3_002824"
     CDS             complement(1479..>2025)
                     /locus_tag="ACG8V3_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301280.1"
                     /translation="KFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQGTYSNEQM
                     ENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKT
                     IVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFIS
                     IITLIVLLFYCIKLKIRTSHE"
BASE COUNT          720 a          339 c          304 g          662 t
ORIGIN      
        1 ttattctttc tgtaaaaaag aaaggagact aaataaacta ttatgaaaaa aggaaaacat
       61 gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg tgttgtttag ccttagtgcg
      121 ttctctacag gaactattct tggatggcaa ttttacaata atgaacactc tcagatagtt
      181 gcccatgctc aatcagctca aaaaaagttg atactgacta ccaatgaaca acagctcgtt
      241 gaaaaaatcg atcaactatt aagaaaaaat gattatattg gctcaattta cgtaaagaaa
      301 aataatcgca tcatcatgga aaaaggctat ggctatgcga ataaactcac gcaacaacca
      361 aatgatcctt ctctttatta tcagattggt tcggttcaaa aagcattgac tactcttttg
      421 attttaaaac aagtcgagcg tgggcacctt tctttagata ctaagctggc tcaattctat
      481 ccacaaattg ctggcagcca gcaaattact atccgtgact tgctgtatat gcgctcaggc
      541 ttgcaccgaa ctgcttctcc aaccataccg atgactgatg aagaagtcgt tcaatttgcg
      601 attcagcact taaaatttac agattatcag acctctcatt atgattcatt gaactttacc
      661 atattgactg gaattttgat caaattaaca aatcaaccgt atgaaaaatt attgaccgat
      721 gatatcatcg aacctttagg attacagcat acaaaatttt atgaccaagt caaaaattca
      781 tccaatcatg cactatccta tcaaatgtct aaagaggatg attactttaa accactgaca
      841 gaatctgaaa cagagatcca taacgaatta gggactggta atatcagtat gtccgtctat
      901 gatttaaata cgctgtttac acaagtttta tctggaaaac tagtttccaa agacttactc
      961 ttctcattat ggcgtgaaaa tgcgaatgga catccatata gtggtggggt atatagcgga
     1021 gataattata tcctgtcgca aggaaatatc gactcttttg atacatctgc tatatttagc
     1081 aaagattcac aggatgctat catcatggaa agcaatataa aagctgacaa aactatcaaa
     1141 ctgcctactg ctgatctaag aaaccaaatc tatgacctga tggaaggaac tactaagttg
     1201 gcaaatagct gatatgatga aaagatagca aatactactt ctttgaatag catttgctta
     1261 aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca aatctaaaaa tcaatttttg
     1321 ctacagactt taattccaaa taaacagaac agaaagaggt gttcttcttt ctgttctgtc
     1381 caaattattc taagatttct ctttaccaac attatttgac aaagggccta ttaaatctaa
     1441 aatagtatct ttagattgct aaaatatgtg ctagcaaatc attcatggct tgttcttatt
     1501 tttaatttta tgcaataaaa aagaagaaca attagcgtga ttatagaaat aaataatgag
     1561 attttagaaa aaaatgtgta tgaatacctg attgttactt tatttattcc actttcactt
     1621 tttactgtaa caaaaggacc tccttgtttt agtactgtag cattttttga attaacagta
     1681 accgttaatc ctttataccg gaatacaggt aaaactattg ttttgccttt tgaagattta
     1741 aacgtaaatg taatttcatc tttatttgga gaaaagctac tataaataac ttgaccatct
     1801 tctgtaactt gatggttttt caaaagcaat tgatcttttt tatagcctat aggtacataa
     1861 tcaaaattct caaattcttc aagtttattt tccatttgtt cattagaata agttccttga
     1921 aaattcattt ctttcaccat gttaactact gatgaataat ttaaaattga aatagctaat
     1981 attaataata gtataaaatt tcttctcctt gtataagaaa atttt
//
LOCUS       DAZKMB010000203         2019 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000203 DAZKMB010000000
VERSION     DAZKMB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.210"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..570
                     /locus_tag="ACG8V3_002825"
     CDS             100..570
                     /locus_tag="ACG8V3_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0301281.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="ACG8V3_002826"
     CDS             792..1895
                     /locus_tag="ACG8V3_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0301282.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DAZKMB010000204         2003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000204 DAZKMB010000000
VERSION     DAZKMB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.211"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG8V3_002827"
     CDS             <1..378
                     /locus_tag="ACG8V3_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0301283.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG8V3_002828"
     CDS             426..1826
                     /locus_tag="ACG8V3_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0301284.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZKMB010000205         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000205 DAZKMB010000000
VERSION     DAZKMB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.212"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8V3_002829"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8V3_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG8V3_002830"
     CDS             complement(728..1744)
                     /locus_tag="ACG8V3_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0301285.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKMB010000206         1946 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000206 DAZKMB010000000
VERSION     DAZKMB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.213"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(856..1281)
                     /locus_tag="ACG8V3_002831"
     CDS             complement(856..1281)
                     /locus_tag="ACG8V3_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFQ0301286.1"
                     /translation="MHRKVRITRKSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISI
                     EGILRIQGPAFIAQGAHIDIRKNAVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDT
                     LFMDTDSHTIFELNTHRILNKNNKIVIGKHCWIGAKSVI"
     gene            complement(1383..>1946)
                     /locus_tag="ACG8V3_002832"
     CDS             complement(1383..>1946)
                     /locus_tag="ACG8V3_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0301287.1"
                     /translation="PFGFAVLLTILGLVYFMYATDGIAAGEGYKRILMELYLHLKKDR
                     KYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIYKYNTTTGTKELSTACLEN
                     IILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSAIIEFLQLFLRLGTFQISD
                     FVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          628 a          381 c          223 g          714 t
ORIGIN      
        1 aatttaaaat taacattgat tctcctatga aaatatcttt tacctttctc ctgtgaaaag
       61 gaaatttcct tttttatagt gttattgata taatcagaat attcatcaaa ctctgtatct
      121 ggagttaaaa atctacagta acatctataa gctttgctag taatcttctt gggttctctc
      181 tccattgtca agcaacatca aaacacctac ttactattcg attcttttct gccaaaaacg
      241 agtcagcatc actcacaact tatgtgttgc tgactccgac tagtcctttg ttcaatatca
      301 acttattaaa cagatataat atttttcttt atttcgtata ctccaagact tccatctttc
      361 tttcatctta tctctgaaaa aaacacagca aagtgccctt aataaatata cattagaatt
      421 tgcaaatttt ttcatcttaa gatatatcct attcatattc tttacaatcc taatgggtga
      481 ttacaaatct atttagttca agttctacat tataaacacc tttcttgata gtatcatctg
      541 ctgcacaagc gatgtcgatc cacttattag gggatttacg tgctcaaaag tggctattcc
      601 ttttgggaag tccatccata ataatggtcc atggattttt ttagggtctt gtcgttcaaa
      661 tttctcaaaa actttattat acttccatag ttttataatt atttggtttc actttggctc
      721 gttcaaacac caacttgtat ttcttttcaa cgttcgaact ggagcccccc ctataatctc
      781 attattattt tcatgacttt ttactaccgt ggaattacaa gcaacaacaa tattatctgc
      841 taaatgaaca cctttctata tcacgctttt tgctccaatc caacaatgtt tacctataac
      901 tattttatta tttttattca aaatcctatg agtattcaat tcaaaaatcg tatgtgaatc
      961 ggtatccata aataatgtat cccacgatac tgtacagcca tctttgaatt gaatttcctt
     1021 aaagcaaatt atactcattt ttcctgtcga attaaacttt gacccaatat ctaaaactgc
     1081 atttttcctt atgtcaatat gagccccttg tgcgataaac gctggccctt gtattcttag
     1141 tattccttca atacttataa tcgtttcttc atttcccgca gtatttaata ctggaaagcc
     1201 caaataaact atttttccag attgtaatac tatctcaatt ttccctctcg atttgcgtgt
     1261 aatccgtact ttacgatgca ccaaaatagg taatctccac gcatctgaaa tatgaagaaa
     1321 tttataattg aagtatatcg ttttaggtaa atttaatata ttctttatta tcaatccgag
     1381 tctcatcttt caccatctct ttttgtaatc aaaattagtt tataaaggat tacacccaat
     1441 ataccgccca acgtattata tacaaaatcc gaaatttgaa acgttcctag cctcaaaaaa
     1501 agttgtaaaa attcaattat tgctgaaaaa taaaatgcaa tttttcctcc atatactatt
     1561 gaggaccata ttttgggttt ctttatattt tcatatttaa tagacattac catataaaca
     1621 tatggaataa aaagaataat attttctaaa cacgctgtag ataactcttt tgttccagtt
     1681 gttgtattat acttataaat ccaccagtct cccataacat cagataaagg atttaaccaa
     1741 attgttcgat tgattaatgt tgtaaataaa atcatcgcag tatagaatat caaagcacaa
     1801 acttttctat attttctatc cttctttaaa tgaagataaa gttccattag tatacgttta
     1861 tagccctcac ctgcagcaat gccatctgtt gcatacataa aatagaccag tcctaaaatt
     1921 gtcaataaaa cagcaaaccc aaaggg
//
LOCUS       DAZKMB010000207         1910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000207 DAZKMB010000000
VERSION     DAZKMB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.214"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACG8V3_002833"
     CDS             14..1141
                     /locus_tag="ACG8V3_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0301288.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..>1910)
                     /locus_tag="ACG8V3_002834"
     CDS             complement(1359..>1910)
                     /locus_tag="ACG8V3_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFQ0301289.1"
                     /translation="GDFYEEFYKRLMRFIFVTGVRSEELLALQWKDFDLINGRCSVNK
                     SIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLKTWKKTQQSIGKNIELVFSFDGLP
                     PSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFLIEHNKNIYAISKRLGHASIKTTL
                     DKYGHLYPETNQALANEITRFDI"
BASE COUNT          653 a          340 c          338 g          579 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc
//
LOCUS       DAZKMB010000208         1894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000208 DAZKMB010000000
VERSION     DAZKMB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.215"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            789..1262
                     /locus_tag="ACG8V3_002835"
     CDS             789..1262
                     /locus_tag="ACG8V3_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0301290.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1271..1498
                     /locus_tag="ACG8V3_002836"
     CDS             1271..1498
                     /locus_tag="ACG8V3_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301291.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          614 a          314 c          289 g          677 t
ORIGIN      
        1 tgatcataac gattggctat ttgattgtat caaatctagg aaatatggta tttggtatca
       61 gtaaagaatt cagttggact actacattat gggaatctct attctttttc atcttcgtat
      121 ttttgttaca aaattatcga aaaaaataaa catgtgccat ctatctgttg gcacatgttt
      181 taggatttat ggtacttttt attcggcaat ccattttatt tttctacttc ttcttaatac
      241 ctctaacact tttgatttat aaactaatcc aatgcatcta cttgcgctta ttaagctatt
      301 tcagctagat agtaccaccc catgcgtcaa tgcttgattt tccttttgta taacattgta
      361 taagcaataa aggacaaata agtggtgatt acaccaaacc attgaataag aaaaattctg
      421 atacgtaaat aaacatgaac ttgaaaaaat taatgaatcg gttagctact acgtagttgt
      481 cttttttctc atacagttct gttaacttac acttctcttc gctagataat aaaaaatcct
      541 ccaactgtat tgtccaatat aatcccaggc atctaaacta gcattataga agagacacac
      601 atagaatcta aaataactgg ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc
      661 actccacacc ttcatactct atcatttttt ataaacgata tatttttatt gtttttcgat
      721 aaaaaatagt tgttttacaa taattattct gctatatttt agctatcaaa gataaaagag
      781 ggataaaaat gaaatttaaa acattgctat taaaatttgt cgttttaggt ataagtgccg
      841 tatttatttt gttcggcgta cttttttata ctcagatcgc tacaagcgaa aaaggattca
      901 attttgattg gcagaccaca ttatttgttt ctgtcattta ctttgccctt ctcctaggga
      961 tcgttattgc ttatttctta ttgaaactat tgaaaaaaat cgaagataac caagcttttt
     1021 ccactcaaag cttaaacata ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg
     1081 ccttttttgg cttacttcct aaatttatga aagtagcgga tttagacgat gctccaggtt
     1141 tgatgttggt aggaatcgct gtcgtattca ttccttttgc agtatacaca ttaacaagcg
     1201 tgctggaaaa actgcttgaa catgcgattt tacttaagac agatcatgat ttgacagtgt
     1261 gaggtgcgac atggcaatta tcgttaattt agatgtagta ttagctcaaa aaaaaatgag
     1321 tgtgactgaa ttgagtgaga aagtgggcat tacgatggca aacatctcta ttctaaaaaa
     1381 tggaaaagca aaagccatcc gtttttccac tctcgaaaaa atctgccaag ttttggagtg
     1441 tcaaccaggt gatttgttga cctataaaaa tgactcgaat actataccag aagaataaaa
     1501 taaagtagaa caaacagtaa ataaaaaata atctgaatga tagatgtgtg agacttttag
     1561 aagttattta cgtctattat tcagattttt tatttcgatt gaataccgtt ttatctataa
     1621 ataagatatg tactgactca tacaaagggg gccttttctt ctccatgtac tatcactttt
     1681 cctgatacaa ctggtttcct gttacgttgt agttggatct gcttttagta taatacttta
     1741 ttcattccac cagactgata gcagtttgag cagtctaaat aatcatgtag tattacaggc
     1801 cattctgttt cataccttga agtgcttaaa acttcagcta atggcccatt gtgttaactc
     1861 ttactccatt cttttttagt aagctcccat atat
//
LOCUS       DAZKMB010000209         1834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000209 DAZKMB010000000
VERSION     DAZKMB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.216"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..259
                     /locus_tag="ACG8V3_002837"
     CDS             14..259
                     /locus_tag="ACG8V3_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0301292.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            335..700
                     /locus_tag="ACG8V3_002838"
     CDS             335..700
                     /locus_tag="ACG8V3_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0301293.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(761..1324)
                     /locus_tag="ACG8V3_002839"
     CDS             complement(761..1324)
                     /locus_tag="ACG8V3_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301294.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          565 a          348 c          284 g          637 t
ORIGIN      
        1 gaaaggtgat taaatgagta tcgaagaagc tattttactg gatactttac gttgtcgtct
       61 gaatttctta agaactggcc gatctttatc tcaagtagct gtttctagcg cgttaagtat
      121 tcctttattc aaatatcgta agtacgaaga aggagaagaa attccaagca tcattgaatt
      181 aatctatatc gctgactttt acgatatttc cgttgattac ctaattggtc gctcagaaac
      241 agcaagcaaa ctgaagtgat tttttccagc cttggtgctg gttttttcat ttaacgtcta
      301 aggttaacaa tctatatata aaggagacat ttctatgcga ttatggcatg aaaaattgat
      361 ttcaaagctt cctcgtcaac agcttttagg acaacatcgg gaatgctgtg cattaagagg
      421 aaatggctgg ggaaagaaac atgctactgt ggattatgtt tttcactatt ctccttatag
      481 attattccaa tatcatcagc ttgtcatgga tgaaatggaa aaacgtggtt acagcccagc
      541 accagagtgg aaaaatccta tgtatcgtgg caaatcatgc gaagcatttt ttgatttacc
      601 tactgtagag cgcaacgtgc cgatttatcc ggaacacgat gaagtttatc tagcagaatg
      661 tttagcaaat ttggagcaaa aaggaattta tcttacctaa tataactaga aaagccttct
      721 atctcctctt acttcattaa aaagctttaa ccatcttaga tcaagctaaa atgtcgtaag
      781 ttttagggtt atcttcatcc tcacgtgcaa aactaaaagc gtactctagt tttggttcat
      841 cgccaacttc cctagacata aatgaacatc tcctgacgtg gtttaaaagt accgcatctt
      901 cgacgtctgt atacgatgca cagcacagat tgcttctttt gatctttctt ttgatttttc
      961 tgttgcttct tctacacctt cttcgattac tacatcgatg cttacaattg cttctgccta
     1021 atattcccat gcatcgtcga tttaagcctc tgtagtcact gtgatcgttt ccacatttta
     1081 actcctctgt cgtggcttca gttgattttg tgatcagttc agttgctaag ccagagtcag
     1141 aacaacctgc cattgctagc gttaatattg atagtaaaaa tagttttttt atcataaggt
     1201 tccccccctg aaaatagact aaaataataa cgctttcttt ttttgttttc agacgaaaat
     1261 taataatttc taaatattct ttaaaaaagc ttataactat tatgcctttt ttaatatata
     1321 acacagttat ttttaaaaat aagaatgcta aatcactcta caataacggc gaaagcacaa
     1381 aaaataaaaa aattattacg ttattaaaaa aacagatgaa agatcaggtt ttattagatc
     1441 tcattctttc atcacttgaa gggatttaat gaactacttt ttcctctttg tcgtttttat
     1501 atattcgtat tccttcaaac agtaggatag cctctttcaa tgcttgatat ttgaactgtc
     1561 cgttacttaa aaatttagat gtgccgccct cttcctagaa catattcttt tttacttttt
     1621 cattaattga cagaccaatc gaacgaaact gtaaatttcg ataggataat gattggtcaa
     1681 actcattcat tttcaagaca tctcgattca tattcaagat aggaatcttt ttatcaacat
     1741 cactttattc gtattttcct ctaccataaa agcttcacgc tccacaaaat tgttttatac
     1801 actactattc agcccattca acgaccaatg aatt
//
LOCUS       DAZKMB010000210         1833 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000210 DAZKMB010000000
VERSION     DAZKMB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.217"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..385
                     /locus_tag="ACG8V3_002840"
     CDS             188..385
                     /locus_tag="ACG8V3_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301295.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            491..973
                     /locus_tag="ACG8V3_002841"
     CDS             491..973
                     /locus_tag="ACG8V3_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301296.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1031..1627)
                     /locus_tag="ACG8V3_002842"
     CDS             complement(1031..1627)
                     /locus_tag="ACG8V3_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0301297.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          566 a          275 c          366 g          626 t
ORIGIN      
        1 tatgttttaa ttttttgaaa aaaattcttc taaatacaac agctgtttta attgtgaaaa
       61 cgaaaacagt acgctataat gcctgtaagg ttgattttta actgtaaata aaaatcaatg
      121 agttaaatct ccaaagaaat agcagtggta atgtggagaa aagttccagc agtttaggag
      181 gattttgatg acggaagaaa tgattaattt aggagaacaa tatgcatgca aaccaattgg
      241 ttttacaaaa acggtcattg gtgaagttgt atcaaaaatg acaaactgcg ccgtggtcaa
      301 agttgcccaa tgtgctgcag aggaccaaga attattagat gaaaaagcaa gcatggttgt
      361 tgcaaaatat gatacatttg aataaagaaa acaattggac gtgcggcgtg ctgcaagtcc
      421 aatttctttg agaaggtaaa tcagtattct tttcctattt tctggagaag aaaaagcgag
      481 gcgagttgtc atggtaacat tctttacggt tctattgttt ttattcgttg ttgtgatgtt
      541 tttatttctt tggctggttc gaaaagaaat tatttatcgg actgtgcgaa atcgatgggt
      601 ctacttggtg atacccttct tagtgtcact agtcatttgg tatacactca tcagtcatcc
      661 tacagcagat gaattggcaa aagggatctt gtcagcaatg atttttatta gttttttgtt
      721 ggacagtaga ggaatcacag aagaaggact tgttttgaat agctttgaca aaaaaggtgt
      781 tcctttttct gagatcaata aaattgtatt atatcagcca aaaggttcta aaatcgtaaa
      841 aatgaatttt ttccgaaacg gatggcgagg accgatgctg aaattttctg catcattaga
      901 agaattagtt ccatttttgt cccaacgctt aaacgatgag gcagagattg atattatgat
      961 tgatccagaa taaaaacaga ggttgcgaca agatgtttgt cacaacctct gttttttatt
     1021 tcaaataaaa ttatccaaat acgcggaagc gatcggcatc gttcatgtat tctttttcct
     1081 cagccggtgt ttctggtgaa ccgaatacta attgcgaacg tagtttccag tttgaaggga
     1141 tattccattc ttttgcaact gcttcgtcaa taactgggtt gtaatgttgc aagtttgcgc
     1201 ccaatccttt atcaactaat gctacccaag tattagctgt tgcgattccg tttgattgtt
     1261 ctgaccaatc tgggaagttg tctgcatata attcaaattg ttcttgtaga ccttttacca
     1321 cgtcagtatc tttgaagaat aatactgtac cataaccatt tttgaaacca gctaatttgt
     1381 tttgtgtatt tgggaaagct tctgctggag tcaatgggcg aagtgcttct tcagtgattt
     1441 cccaaagttt ttcatgtgca tctccgaaaa ggataactgc gcgtgttgat tgagcattga
     1501 aagcagttgg gctttcttta attgcttctt tgatcaaagt tgttaattct tcattagaaa
     1561 gtgaaacatt acgtcctaga tgatagattg aacggcggtt tttcaaagtg tcagtaaaag
     1621 ttgtcataat aaaatttctc ctttgatttg cgttttgcat ttttttaatt gattacgaaa
     1681 tgaagtatag caccttacaa aaagtaagta aacagatatg ctcacaaaaa ataagcgaag
     1741 ttcaaaaaac aattttaata agaaaaatcg ttatgtaagg attttcttag ttatcgcttt
     1801 cattgttgtg ttttaggaat ttttgcttat ttg
//
LOCUS       DAZKMB010000211         1819 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000211 DAZKMB010000000
VERSION     DAZKMB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.218"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..233
                     /locus_tag="ACG8V3_002843"
     CDS             <1..233
                     /locus_tag="ACG8V3_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301298.1"
                     /translation="GDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIFMIVIWFLNE
                     RSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(485..1057)
                     /locus_tag="ACG8V3_002844"
     CDS             complement(485..1057)
                     /locus_tag="ACG8V3_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0301299.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1073..1678)
                     /gene="fic"
                     /locus_tag="ACG8V3_002845"
     CDS             complement(1073..1678)
                     /gene="fic"
                     /locus_tag="ACG8V3_002845"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0301300.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          536 a          367 c          271 g          645 t
ORIGIN      
        1 aaggagactt gaatgccatt attactttaa tcgtagttat ggtaaaatca ataggtaata
       61 tcacaatagg aatgctgttt attatattag cagcagtgct gatttttatg atagttattt
      121 ggttcttaaa tgaaagatct aaagacaatc aacaccgtag ggatatggag ataaaatcaa
      181 tagagtcaat agattattta tcgataataa cactaaaaaa gccacgttcc taaacgtgac
      241 ttacgctgaa cgatatgtgt tgtttggtag acgaacaaat cgttcattat aaatatatga
      301 tggtatccta atatagtaag cgtggctagc atacctttaa ttactttacc ttttacagtt
      361 accatacaca ttttttgagc agaaatcaag taaataaaaa aatattatat ttcacaacat
      421 gctaatagac tcatatctag aacgcattaa gtatttacct gtctggttcg ctgagacttc
      481 ttttttaact ttcttcaatt ttcttcttta ttctaaaaag cgtactgcga ctgaaaccgg
      541 ttatagcttc aacttcctta tacgatttac ctgaagtaag cagttcatat gcgtgcctct
      601 tcttcggtgt gattgttgct ttcggacgtc cttctctgaa attaggatta ttctttcttg
      661 caaacatttt tccttcttgg gtacgttcca caatcatatc acgttccatt tctgctacac
      721 tcaacaacgt ccgtgtcacc attcgcccca tggacgtatt ttcaatcgtt cctagattca
      781 aaaccttgat cgttacgtta tcatcgagta aaggttcaat gatctctaat gcttctctcg
      841 tgttacgagc aaatcgatca agcttcgtaa caactatttc atctccaggc ttcagaccag
      901 ccaacagctg attgaatttt ggccgatccc gttttgtacc cgtaaatttt tctgaaaaaa
      961 tattcttctt ctctacgccg ttctcaagca actgttgaat ttgttctttt gtactttgag
     1021 aagcggttga gacacgtgca tatccatatt tcgtcatttg gtccacctac ttttacaaat
     1081 catctatcgt atactcagaa tacccttcat agaaatagct gacatcgatt cctttcatat
     1141 ataactcgcg atcgtcaatt ttatcagtca gagcattgga aattagatag cgtatttcca
     1201 aatcgtttat gggacttcgc tccattgcag aaagatagtc tgctttatcg attaagttcc
     1261 aatctacaac tttttgcaat tcttccttta aaatgagatc cagccaaatt ctagtacttc
     1321 ggccatttcc ttctcgaaat gggtgagcga tattcatctc cacatatttc ttgataatct
     1381 cttcaaaaga agtttgtggc atctgatcaa tatgctgtaa cgaatgttct aaatacataa
     1441 ctggtgcaaa tcgaaaattt ccttttgcaa tattgaccga tcggattttt ccggcaaaat
     1501 cgtaaatatc cgaaaataga aattcatgaa tttcagataa acctttaaat gtaccaactt
     1561 cgatttcatt tatttttcca caatcgtaca attctttcgc tttttgttta ctgagttttt
     1621 cttcaacctt agccaactca acttgattgg ttaatcccaa tttattctct aaaatcatgc
     1681 gaaatctcct taactttatg acataagttt atgaaacact ctcaacccca ttatatcgcg
     1741 attttgttta tgtgtcaaaa actattaagt ttctgacccc cgatagattt ttcaagtgtc
     1801 tctattttgc acagtctag
//
LOCUS       DAZKMB010000212         1819 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000212 DAZKMB010000000
VERSION     DAZKMB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.219"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1753
                     /locus_tag="ACG8V3_002846"
     CDS             275..1753
                     /locus_tag="ACG8V3_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301301.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          661 a          253 c          325 g          580 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaagg
//
LOCUS       DAZKMB010000213         1814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000213 DAZKMB010000000
VERSION     DAZKMB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.220"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(188..1207)
                     /locus_tag="ACG8V3_002847"
     CDS             complement(188..1207)
                     /locus_tag="ACG8V3_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0301302.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1204..1428)
                     /locus_tag="ACG8V3_002848"
     CDS             complement(1204..1428)
                     /locus_tag="ACG8V3_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0301303.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1425..1718)
                     /locus_tag="ACG8V3_002849"
     CDS             complement(1425..1718)
                     /locus_tag="ACG8V3_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301304.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          513 a          371 c          310 g          620 t
ORIGIN      
        1 ggttgttgcc acataaagcc ataaacaatc aaccagtaga atgtgtggca acaaacctgt
       61 tatcgcatag cttggagtgt gactattatg ggtgatagtg aagatatgcg ataacatcac
      121 tattttatcg aatgattttt atagttgtca atatagttat gtaatgctcc tcaacgagga
      181 gctattttta tcgtttagga atatttaaat accaacgttt gtcatggaaa tcttgcgcac
      241 cgcctttagt gtttccctct ggatcattcg ttgcccgcat catgacatag actttcttat
      301 tagggaagtt acgcatgtta aaagatacat gatagccaac atttccagaa gtattataag
      361 cttgatttac atctgatcta taaattccat cagctcttac tcgagctaat tctttcccag
      421 tattgtaatc cataatgaag atatactcgt atttatagtt agcaatgtgc catccagcta
      481 catgcaaatt tgcattttcg atttctccaa actgatcaat gtgggcgtaa ttcgttccat
      541 ctgtcagtgt aggattagct gcaccagctc gtgttggatc aatgacaggc ttgttttcag
      601 aagttgttgg attttcatcg gtaaatccat gagctaaatc atatgctaat ttttctttac
      661 ttacgcccat ttcagaaaga taaccgtaag gatctgtatg atcgccccag atgttttgtg
      721 ttacccataa atgcgatttg attcccggtt ggttataagg agtgtccaac gttaatggaa
      781 taccatattt cattgctgaa tctctagcca attcaacgta tgccttgtag tttttctcaa
      841 acgttgcttt atcatgtgtg tgttgtaact caatctgcac aggactgttg gcattagcat
      901 acgaaccagc accgtactgt acataaccag gttgaccgac ttgataaaca attccgccgt
      961 ctcccacaat ataagcagta taagcgctag tccatgaacg ttgcatatac tgcgcttcat
     1021 tgcgtcctgt tgctgtttca ttagccgttt catgcagtaa aatatactga ttatttgcta
     1081 cttgtgagct accttcattt gcgcccaaat taaattcatt gttgatagta taggcaaatc
     1141 cgttaattgg caataaaaaa agagccgtta acaggctcat cgcagtaata gtaattttct
     1201 ttttcatttg tttcctcctt cttcgctttc agccgagaac attttgtagg ttcgatttga
     1261 tacacccaac acactcccta aaaacgcgcc aaaaccagta atgatgacaa cacagatatc
     1321 tgtgtactgc caattgagcg ctttaccaac taaccccacg aaagtagcta gtgcgggaat
     1381 aattaccagt gcgaaccatt ttagtacttc gaacgtttta ttattcattt tcttctctcc
     1441 ctaaacaaag ttttgatttg ttgcgtgtgt tccaccaatt tttctgtatg tgtatctaat
     1501 ctttcatcgt gtttctttag ttcttcatga atcatcaatc gatctgattt gctcgattct
     1561 aaatctttag tcagcaaatc taaattgtgg cttacttttg aaagagtctc agtaattttc
     1621 gagaaagatg cagcaatcgg tctaattact aataaaatca aagaaacgat agcggttatt
     1681 gatcctgcta tcattcccca ttcccctaaa ttaatcatgt gacaactcct ttaccttaaa
     1741 taaaaacgca tcaattaaga tgcgctctta tctttattaa tgattttatc tgcttcttcg
     1801 tctgtaatgc ataa
//
LOCUS       DAZKMB010000214         1802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000214 DAZKMB010000000
VERSION     DAZKMB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.221"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..898
                     /gene="erm(B)"
                     /locus_tag="ACG8V3_002850"
     CDS             161..898
                     /gene="erm(B)"
                     /locus_tag="ACG8V3_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0301305.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1068..1328
                     /locus_tag="ACG8V3_002851"
                     /pseudo
     CDS             1068..1328
                     /locus_tag="ACG8V3_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1462..1701
                     /locus_tag="ACG8V3_002852"
     CDS             1462..1701
                     /locus_tag="ACG8V3_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0301306.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT          707 a          273 c          311 g          511 t
ORIGIN      
        1 aaacagacta aaaacagtga ttacgcagat aaataaatac gttagattaa ttcctaccag
       61 tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc gtttaacttc
      121 tgtatttgtt tatagatgta atcacttcag gagagattac atgaacaaaa atataaaata
      181 ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac aattgaattt
      241 aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa cgacgaaact
      301 ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat tcaacttatc
      361 gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata ttctacagtt
      421 tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc atttaagcac
      481 acaaattatt aaaaaagtgg tttttgaaag ccgtgcgtct gacatctatc tgattgttga
      541 agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc tcttgcacac
      601 tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa
      661 agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag ataaatattg
      721 gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac
      781 taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccattac
      841 ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt
      901 ctatgagtcg cttttttaaa tttggaaagt tacacgttac taaagggaat ggagataaat
      961 tattagatat actactgaca gcttccaaga agctaaagag gtccctagcg cctacgggga
     1021 atttgtatcg ataaggggta caaattccca ctaagcgctc gggacccctt gtaggaaaat
     1081 gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt ttatggttgt tcaaattatc
     1141 ctgaatgtaa gtttacttta gctgaacatt ttagaaagaa aaaactaacc aaaacgaatg
     1201 taaaagaatt actggaggga aaagaaaccc tggtaaaagg aatcaaaaac aaagagaaaa
     1261 agccctacaa tgccgttgta aaaattgggg aaaagggata tattgatttt atatctttct
     1321 caaaataaac ataaaagccc tttaaagagg gcttttatat attaatcaca aatcacttat
     1381 cacaaatcac ttatcacaaa tcacaagtga tttgtgattg ttgatgataa aataagaata
     1441 agaagaaata gaaagaagtg agtgattgtg ggaaatttag gcgcacaaaa agaaaaacga
     1501 aatgatacac caatcagtgc aaaaaaagat ataatgggag ataagacggt tcgtgttcgt
     1561 gctgacttgc accatatcat aaaaatcgaa acagcaaaga atggcggaaa cgtaaaagaa
     1621 gttatggaaa taagacttag aagcaaactt aagagtgtgt tgatagtgca ttatcttaaa
     1681 attttgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
     1741 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
     1801 ca
//
LOCUS       DAZKMB010000215         1779 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000215 DAZKMB010000000
VERSION     DAZKMB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.222"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..560)
                     /locus_tag="ACG8V3_002853"
     CDS             complement(<1..560)
                     /locus_tag="ACG8V3_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFQ0301307.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVD"
     gene            complement(565..750)
                     /locus_tag="ACG8V3_002854"
     CDS             complement(565..750)
                     /locus_tag="ACG8V3_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0301308.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(1104..1349)
                     /locus_tag="ACG8V3_002855"
     CDS             complement(1104..1349)
                     /locus_tag="ACG8V3_002855"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301309.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(1343..1744)
                     /locus_tag="ACG8V3_002856"
     CDS             complement(1343..1744)
                     /locus_tag="ACG8V3_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0301310.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT          548 a          328 c          232 g          671 t
ORIGIN      
        1 tcatcaactg tccaaaattc tacttgagga cgctgagttt ttattttacc tatagtatct
       61 acaggatttt cttcaataat ttcaagtaca attgcccttt taaaaactac tctcaactta
      121 ttgaatatct gtttaatata atttggtgat aatggttgct caatgccctt ttttacaaca
      181 tggattttcg ataaaaactg ttgaaattcc tgtatatgaa ttggtcgaat ctttacaacc
      241 tgcatttctc caaaaaaatc caatgcacgt tgtaaaatat gatctgtttt catatatgtt
      301 ttttctacag ttcccaactt ataccaagga aaaaaatact ctcgataaaa atcatgaaac
      361 gttattcctt gcagataaat tccttgtgaa tcagaaaaac tttgttttaa ctcttcgagt
      421 ccccttctag cttctccttg agttttgaag cctcttctgg tcacttgaat tctttttcca
      481 aagcgatctt gtcctaaagt tgcagaaaaa aaccaagtgc ctctttttct gtctttataa
      541 acattcgcaa ttttcctcat tttactattc ctcccaagaa atatggagaa cttcttctaa
      601 aattgaaata ggaattcttt ttatttttga attacgataa aaaaagtatc ctttctcaac
      661 taaaagttct ttacattcct tgaagatttt attagcatag tactgagaaa aacctagatt
      721 aatcaaatct tttcgagtag ctgttttcat tatagcacct tccttacttt ttttcattta
      781 tttctatcag ctgcttttgt caaaagcaca taggaatttc acctctcccc aagtttacgg
      841 cgagccttac ggaagtatca ttatcccaac tttaaatcat cgctttttat tgactataaa
      901 aattatttta gaatatttat cgctccttct ttacagcaag aaatcatggc gcaactaaaa
      961 tatgctcctt aaagtgttaa cgtactgata gaatctattt aattttcaat gaacaatagg
     1021 ttttctacct aaaagaatct actaattcct taactattag aagattcttt taaacagaaa
     1081 aagtggaaag gggatttttt taattagtta attttaaatt ttaagatcgc ttctattaat
     1141 ttaatttcta gctgacttat catatattca tctacatagg taacgaattg acctacctcg
     1201 ttaaatccat ttgtcaaaca taacttagcc atataagaat gataatgttt gagtatcatc
     1261 ttgatcgaaa ttggatctcc ttctagagca gataagataa tggatagtgg aactaatagg
     1321 tactgccgct ttttagattt atctaccata ggtcataccc cattttcaat tgttcaactg
     1381 ccttctttcg ttgataccag ataccacttg tgctcatatt gagaatttta ccgatttcag
     1441 catcattaaa acctgcaaaa taataaagta ggataatctt tcttcttatc tccgataact
     1501 gatcaattgc tgctgataac ttttgatctg aaattaatat ttccattcca aataagaaat
     1561 agacatcttg ttcatcaaaa tgagtttggg aatacctgcc ttcatttgaa tctgccaatg
     1621 gttcctcatt atcctttatg ttagcgtttc gttttcgaat agacttagct tcgtttcgta
     1681 atactttttt acagtaggca tcaaattggt gttgtataga tatgtcattg taatgttgtt
     1741 gcatgataat atgctccttt ttcccccttt ccactaata
//
LOCUS       DAZKMB010000216         1770 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000216 DAZKMB010000000
VERSION     DAZKMB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.223"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..598
                     /locus_tag="ACG8V3_002857"
     CDS             <1..598
                     /locus_tag="ACG8V3_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301311.1"
                     /translation="YFVYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVG
                     DKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSS
                     LPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKE
                     TLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            585..>1770
                     /locus_tag="ACG8V3_002858"
     CDS             585..>1770
                     /locus_tag="ACG8V3_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0301312.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFT"
BASE COUNT          582 a          229 c          284 g          675 t
ORIGIN      
        1 ttattttgtc tattgtccgg aattgttact ttatgatgta aaaaatataa aaaaaattcc
       61 caaaatagat gaaaacagta aattgatgaa aaacatacaa tcaatctttg ggatagaaga
      121 agtttttgtc ggagataaat gtatcttttt tgatcaacct ttattatctg atggttatgg
      181 catagatgaa acagatattt tcgctaaagt aaatagtata ttttctggaa aaaaagaaac
      241 tttaattaag cttcatccta gagcaaggac aaatcgttat ggtgaaaaaa acatattgaa
      301 ttccagttta ccgtttgaag tcttgctact tgaaaatgac ttctcttcaa cacttttgat
      361 tagtcctata tctacaattt ctttttcacc atctatgatg tttgaaaaga cgatcaactc
      421 tattatgttg gctaaattaa ttttaaatga atatacagca ttatctgaag ataaaaaaga
      481 aacattgaca aatattgttc ttttctgtga acattataac cgtctatcag aagaaaaaat
      541 tttactccca gaaaactggg cagaattaag aaagttggta gagtatggga ttatttaaca
      601 cactttccaa gtcaaaatta gttaagtcca gtttctggta tactataggg aattttttta
      661 ttaagggaat ttcattcata actattccta tttttgtaaa tattactact caagcagaat
      721 atggtttgat taataatttc acttcatttg tatcaatttt ttcgattctt gttgggctaa
      781 gcctaaattc ggcaattaat aatgcaagtt ttgactataa agatgacata aatgaattta
      841 tgtcatcggt actttctcta gctacacttt cgttaattgt gttcttatta atagggaaca
      901 tatatttttt gttttcaaat gctttttttg atatgaacca acttgtattt aatttgatgt
      961 tattacaaag ttttgctagt tttgtaatta gttatatcca gtcttatttt acgattaact
     1021 ttaaccattt taaatatttg attttatctt ttatatctac ttttttgaat attgcaatct
     1081 ctgtagcttt gatgcagaca ttatattttg atgataaata tgttggcaga gtagtcggcg
     1141 gaacactgtc atttgtgttg ataggtttag taatttattt aagaataatg ataaagggtg
     1201 ctcatttttt tgataaaaaa cagtggagat acgctttaaa aatctctttg ccgctaatcc
     1261 cacattcctt agcaaatata gttttagcac aattcgatag aattatgatt aattcttatt
     1321 taggtcctgc tgcgggggga atatattcct acattagtaa cataggtatt attctaagcg
     1381 taatatggat gtctactaat aacgcctggg ttccatggtt ctatggtgag atgagtaaaa
     1441 ataatgaaaa aaaagtgaag aaagttagta actattattt agtagtcttt actttgatta
     1501 cgatgattgt aatggtagtt tcgattgatt tagcaaaatt tatggctccg aaagactatt
     1561 attctgggtt atctttagtg gttccgataa ccttaggcta tttctttcag tttttatata
     1621 gtttaccagt taatgctgaa ttttatgaga aaaaaacatc gtttattgcg attggtacag
     1681 tagcaagtgc tatagttaat atactattaa acatgatttt catacctagg ttaggaataa
     1741 ttgctgctgg atacacaacc gtatttacgt
//
LOCUS       DAZKMB010000217         1742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000217 DAZKMB010000000
VERSION     DAZKMB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.224"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1021
                     /locus_tag="ACG8V3_002859"
     CDS             <1..1021
                     /locus_tag="ACG8V3_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0301313.1"
                     /translation="FVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYT
                     PVGELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIIS
                     AIMSIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPF
                     MIIWVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIV
                     LNIILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIA
                     FVASYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTS
                     VLVKR"
     gene            1199..1420
                     /locus_tag="ACG8V3_002860"
     CDS             1199..1420
                     /locus_tag="ACG8V3_002860"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0301314.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          585 a          178 c          342 g          637 t
ORIGIN      
        1 atttgtaatg caggcatatg ttctgatttt cttaaaaaat tactatttgt atttattggc
       61 ttctattttg ggacaaatag ttcttaactt aatgtgtgct gttgtttctc aaaagatgta
      121 tcctgattat actcctgttg gtgagttaga gaaagaaact attgacgata ttataaaaaa
      181 agtaaaagga ttagttacaa acaaggtagg cggtacaata ttacgatctt cagattcaat
      241 tattatttca tctttcttag gtctttcagt acttgcgatt tatcagaatt attactttat
      301 tatttctgcc ataatgagta tatttgcaat aatttatgaa tcatgtatag caggtatagg
      361 taacagtctt attgtagaaa gtcaagaaaa aaattatata gatttttgtg atattacaac
      421 aattataggt tggtttctgg caattagttg tgcatgcttt attacattat ttcagccgtt
      481 catgattatc tgggtgggaa gtgataatct tatgagtatt tcatttgtta tatttatgat
      541 agttcatttt ttcttatatg aaattgatca gttaattggg ttatacaaag atgctgctgg
      601 aatatggtac gcagacaggt ttagaccatt aattacagca atgataaaca ttgtattaaa
      661 tatcatcctc gttaattggt tcggattata tggagttttg gcggcaacca ttgtttcagt
      721 attgttggtt gatttaccct ggttattata taatgttttc caaaatatat ttaaatttga
      781 atcggcaggt aaatatgttt ggaagctcat aagtttgtgt gtaatagctt ttgttgcttc
      841 ttatttaagt tattttgctt gttcgtatgt taatatcgga aatttcaaag tgataagttt
      901 agtgataaat ttagttatat cggttgtcat atcgatttta gttattcttc tgtttaatat
      961 ccgtaataag aagtttaagt atattttgaa agttcttacg tcggtattgg taaaaagatg
     1021 agaagagaca tcagaatgat atataaagta actacaattt ttaggctgcc gaataggcga
     1081 acgatgtata gtttttgttc caacgaagac tcttgttgaa attggagatg atatgaagga
     1141 gttacaattt tgactcatgt ttatgattga tcggttttaa aagtagtatg gagaagcttt
     1201 gggatcatcg gagaggatca aaataggtaa taatgttttt ataggaatga agtctagaat
     1261 actaaagggt gtacatatag gaaataatgt agttataggt gcaaatagtt tagtaaacaa
     1321 ggacgtatca gacgattgtg ttgttgcagg aaatctagca aaagtaatta tgtccctaga
     1381 tgaatattat aaaaagataa acaatatgaa gaatccgtaa aacttgtgaa actataccga
     1441 gaataatatg ggaaaaatat tgatgaaaaa gcattatatg agtttttagg gctgtttaca
     1501 gatggagatg acaatttgtc gaagagttgg gagcaatgca aaatttagta ggggattata
     1561 aacagactga caaggtattt aaagaacatg cacaagtatt tcataatatt gaagaatttt
     1621 tgaatactgt gagataggag ttgctacatt gagaaaagta tgtatttttc caatgtaaaa
     1681 gattacgagt ttgaaaacga caccataata ttatttttta agaagatatt atagagctta
     1741 gg
//
LOCUS       DAZKMB010000218         1738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000218 DAZKMB010000000
VERSION     DAZKMB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.225"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1508)
                     /locus_tag="ACG8V3_002861"
     CDS             complement(27..1508)
                     /locus_tag="ACG8V3_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301315.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          540 a          233 c          301 g          664 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
     1321 tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt
     1381 cggatcagtt ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa
     1441 gaacaattat ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca
     1501 attagcataa atacctcctt tttattctgt ttttgtatat agttagacaa aaactgtgag
     1561 tggaaagtca tttcagtctg tgttattctc acagagtgtc gccttctttt ttatataacg
     1621 ttatttaatt tttttgttac cttgtttatt ctattgttta tttcctaggg tattttgtca
     1681 agatattctt gtttttaagg cggaaaaatt gatttttaat ggatataact aaatatgt
//
LOCUS       DAZKMB010000219         1646 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000219 DAZKMB010000000
VERSION     DAZKMB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.226"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..183)
                     /locus_tag="ACG8V3_002862"
     CDS             complement(<1..183)
                     /locus_tag="ACG8V3_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFQ0301316.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFF"
     gene            complement(197..1447)
                     /locus_tag="ACG8V3_002863"
     CDS             complement(197..1447)
                     /locus_tag="ACG8V3_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0301317.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
BASE COUNT          491 a          368 c          267 g          520 t
ORIGIN      
        1 aaaaaacttt tcttttctat ttacttgtct catgtagtac gttttttcgc cttttgactc
       61 agctgaatat tctgtctgtt ctgttataac aaaactctca tacttctcca tcagtggttt
      121 taacatcatt aactgttcat aatgcccccc agaggaagcc gcaaaacata tctttttgtt
      181 catattaacc tctctatcag tcacgctgaa aaatatctct agtgtaaact ttttctttaa
      241 tatcatccaa gcaggtatca tatcgattgg caataataga atcacacatc gccttaaact
      301 gctctaaatc gttgataacc ttacttccga aaaaagtctc tccatctttc agtgcaggct
      361 cataaatcac cacagtcgca cctttagctt ttacgcgctt cataactccc tggattgaac
      421 tctgacgaaa attatcactg ttgctcttca tggtcagccg gtatataccg attaaaactg
      481 gattttccct gttgacatca tactgattat attcagcgta gttgtaatag cccgccatct
      541 gaagaacacg atctgcgatg aaatccttac gggtacgatt agattcaacg atagcctcaa
      601 tcaaatttcc aggaacatct gcatagtttg ccaacaactg cttggtatct tttggcaagc
      661 aataacctcc atatccaaag gatggattgt tgtagtgagt tccaatacga ggatcgagac
      721 aaacaccatt gattatctgc tgagtattca gccccttcat ttcagcataa gtgtctaatt
      781 cattgaaata gctgactcgc agtgctaaat aggtatttgc aaacagcttt actgcttcgg
      841 cttcagtgaa tcccataaaa agagtatcca cgttctcctt aattgcactt ttctgcagaa
      901 gtgttgcaaa aacatgagca gcatttaaca agcgctcatc agtcatatca gtaccaacaa
      961 taatacgaga tggatacaga ttatcatata aagccttgct ctcgcgcaga aactctggac
     1021 taaaaataat gttcttactc cccgttgtct cacggactga ttcagtgtag ccaacaggaa
     1081 ttgtagactt gatgaccatg atggtatcag gattcacgtc catcacaacc tcaataactg
     1141 actcaacagc agaagtatcg aaatgctgag tacgactgtc atagttagtc ggtgtagcaa
     1201 taacaacgaa atctgcggat ttataagcgc tccttgcatc caaagtagca gttaagtcaa
     1261 gttctttttc tgtaagatac ttttcaatat attcatcctg aattggagac ttccgtttat
     1321 taatcagttc aatcttttca gggataatgt ctaccgcggt tacctggtga tgctgcgcca
     1381 atagtgtggc gatactcaaa ccaacataac cggtgccggc tactgcgatg ttgtactttt
     1441 tcttcacaat atctcctctt tcctctctta agtatggaga atgttactcc tacggctatt
     1501 catcatgtca aaataacaca gcttagaatg ttgataaaat aattttccag aatgtgataa
     1561 aatctctttc atttataaac cacacatcta attttatcta ttctccacaa gacatgtaga
     1621 gtattcttta ataacaagat acgacc
//
LOCUS       DAZKMB010000220         1642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000220 DAZKMB010000000
VERSION     DAZKMB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.227"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..193)
                     /locus_tag="ACG8V3_002864"
     CDS             complement(<1..193)
                     /locus_tag="ACG8V3_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0301318.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTG"
     gene            887..1273
                     /locus_tag="ACG8V3_002865"
     CDS             887..1273
                     /locus_tag="ACG8V3_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0301319.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1367..1558
                     /locus_tag="ACG8V3_002866"
     CDS             1367..1558
                     /locus_tag="ACG8V3_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301320.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          612 a          248 c          224 g          558 t
ORIGIN      
        1 caccagtaaa gaaatggaaa tctttagttt ttttaatatt atcagttagt aactttttat
       61 aatcataaaa aaaataagta gttctatctg agcgatactc ttttataata ctgaattcgg
      121 aattaaaaac cacgataggt aactttaaac aaacatgaag agactttaat aagacttctt
      181 tatctgaaaa cacttttgtt tctctctttc ctttctacat cattaaatag tattttatac
      241 gttatctaaa atactattcc tctgtcaaaa taagctaact tacttcatta taaaatatcc
      301 acttatcgct ttcatgtata ttccaccgta aaaagtacat tttatcaccg agaactcatc
      361 atttttcatc atgatgaata ctatttacat atatctagtt caaagacaat aattattatg
      421 atccgttatt atctaagcac tatacagata ataggaattt ttcgtttcta aatattccaa
      481 tttgggaatc caataaaaat tttcgttcaa atacgtgtct attcgaactc ttttctagtt
      541 gttaattatg aagggggaag aaataaaaaa tacaatctat acacgaataa gaatattatc
      601 caaaaggagg ttttagaaaa gatttattat acgtaaaaag ctcaagacat tttcttagcc
      661 ataaggacat gtttgatatt taagaagata tatgaatttt cttttctaag tataaagata
      721 actatccatt atcaactaaa aaaacatttt tgaaaataaa catcagcagt cctatcaatg
      781 catgtgagct tatagaagtt attcgcagcg cagcataatt gcattagctt cttttcgtgt
      841 tttttgcctc ttttcccttt aaatcttaat ggaggaggtg tgcaatatgt cagataacga
      901 cgaacgcgta aatgcccagt tgattcgatt ctttgaaaag gtcattttga acaccgctaa
      961 tacttattat aaaaaacaag aagaaattca aaaacatgaa caacttgatc aactcccacc
     1021 atatttgttt actaaagata ctataaaatt taaacaaccg attaatgttt taaatgtgac
     1081 tctccttgtt gaagatataa aattagcaaa aatccttcct ttgttgaaag aacctgaaca
     1141 aatttttctt atcgaaaaat ttatctttga taaaactgat aaagaaattg gaaaggttct
     1201 ggggattaca agacagggtg cgacaaattt aaaacatcga ctttacaaaa aaattcaaaa
     1261 aatgctggtt taatatctgc ataataacta attttgtatg cttctttgct cgttctcctc
     1321 cttcccaaag aaatagatta taagtccatg ggaagaaggt agatttatga attctttaaa
     1381 agaagatttc attttagcaa aggctggaaa tgaagaagct gttgaggcaa ttctcaaaag
     1441 atttagctct ttgattcata agcaatcctg gagaactgga aaatatgatc aagattgtta
     1501 tcaagaatgt atgcttgcaa tatatctagc tatctcgaaa ttcgaaatta aagaataact
     1561 caactaaggg agtctgtgac ataagtaaaa agatagtttc aaatttcgaa caataaattt
     1621 gaaactatct tctttataat ta
//
LOCUS       DAZKMB010000221         1616 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000221 DAZKMB010000000
VERSION     DAZKMB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.228"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..476
                     /locus_tag="ACG8V3_002867"
     CDS             54..476
                     /locus_tag="ACG8V3_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301321.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            486..689
                     /locus_tag="ACG8V3_002868"
     CDS             486..689
                     /locus_tag="ACG8V3_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301322.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            900..1484
                     /locus_tag="ACG8V3_002869"
     CDS             900..1484
                     /locus_tag="ACG8V3_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0301323.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          570 a          227 c          297 g          522 t
ORIGIN      
        1 ttaagattta ttaagaaaac gttttcttat ttttgatttc gaggtgatag acaatgaaaa
       61 ataagtttat tttatcttta ggtattttaa ctattggaat tagtgtaact ggaactacaa
      121 ttcctacatt tgctgatagt acaaatgaac caactactgt agtagataat aaccaagaat
      181 taactccttt agataggaac ggaatagcca ttggtaaccc aactaacgac ttaactgtta
      241 ctgaaaatta tttaatagtt tctccattac ttgctcgagc agcttctaaa acgatacgag
      301 tgacaggaac gatgattttt gataaagttg gttggccttc tagacctcct caatcacgga
      361 attggaaaga aaaaagaaat ggttattggt actatggaaa attgtattta caaagttata
      421 aatcagctgg aaacaattgg attgcgactt attcaggaac cttaactaga aagtagggat
      481 tttttatgcc agaatttgtg aacacaccaa atttttatca agatagcgat attctaagta
      541 aaattaattt accgaaagaa gaatcagctt tacaagttca gcgaacttta caggagacac
      601 aaaaaattca aaaatcaatt tctaatctag aagattctga agaaaaaaga aaaaaaagag
      661 accaaaaaac atatcaaaaa gaaaaataat gattttaaaa aaagagtgtc tagtcgacat
      721 tctttttttc taattgaaag tgttcataaa atcacccatt tctggacact ttcttttttt
      781 gctttatttt tcaaaaaaac aattcatttc atgttcataa acccataaca atatctatgt
      841 tcagttaaac tatatggata aagttatgtt acaataaaat taatgaagga gtgatttttt
      901 tgaaaatagg atacgcccgg gtatctactg gtttacaaaa tttggattta caagaagaca
      961 gcctaaataa atttggttgt gaaaaaattt ttactgatca tatgagtggg gcaaaaagta
     1021 atcgaccagg attagaaatg gccattgatt ttgttcgctc tggcgataca cttgtcgttt
     1081 ggcgcttaga tcgtttaggt agaaatatgg aagatttgat ttcaatcgtc aatcgactga
     1141 atgagcgtgg tgtgagtttt catagtattc aagaaaatat tacgatggac aaatcaagtt
     1201 ctacaggaca gctcatgttt catttgtttg cggcatttgc agaatttgag cgtaatttga
     1261 tcctggaacg atccgcagca ggacgagcag ctgctcgtgc aagaggacgg tttggtggtc
     1321 gtcctgaaaa attgtctgaa caagatttgg aattactgaa gactttagtg gatagcggta
     1381 cacctattaa gactattgcg gaacggtgga acgtctcaag aaccaccatt tatcgttatt
     1441 tggcaaaaat ggaggagaaa aatggaaaga caaattctca ttagtttttg tcatttaatg
     1501 ctgaaaattt ttgccattta tctagaaaag tgacaatagt atgagaattt tttgtaagtg
     1561 tctactgata acttattctc aaatgttgta aggattttat tcatttgtta atatta
//
LOCUS       DAZKMB010000222         1577 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000222 DAZKMB010000000
VERSION     DAZKMB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.229"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACG8V3_002870"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACG8V3_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACG8V3_002871"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACG8V3_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          555 a          238 c          210 g          574 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa ttttttt
//
LOCUS       DAZKMB010000223         1566 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000223 DAZKMB010000000
VERSION     DAZKMB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.230"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..710
                     /locus_tag="ACG8V3_002872"
     CDS             39..710
                     /locus_tag="ACG8V3_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0301324.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(870..1061)
                     /locus_tag="ACG8V3_002873"
                     /pseudo
     CDS             complement(870..1061)
                     /locus_tag="ACG8V3_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          570 a          243 c          213 g          540 t
ORIGIN      
        1 aaaatatcag ttaaaataaa agttaaggag cattgtgtat gacaattaaa actagtctcc
       61 aaaatcaagc ttaccaatct attcgccgac aaattatcta tgctgactta gaacctggaa
      121 aaaaagtatc agaaaaaaaa ttagaagaaa cactttctat tggtcgtaca ccaatacgtg
      181 aagcactgat tcaactccga caacaaggtc ttgttgatac aattcctcaa agcggaaatt
      241 atatctcatt aattgattta gattcggcca aaaatgctcg atttgtcaga gaacatttgg
      301 aacgacaaat tatgttagag tgttgtgcca aaatgaataa ccaaaaaaaa cggattttag
      361 aaactataat ttcagaacaa gaaaaagctg ttagtcaaaa aaatgctcgt agtttttttc
      421 ataatgataa tttattccat aaagcttgtt ttgaaattgc agagcgagaa gaagtatggc
      481 aatggttaga aaataacaat acacacttag aacgatttcg atggctacga gtaaaaacgg
      541 aaggattaaa gtgggaaaaa attatggaac aacattatca actatttgag gcattaacaa
      601 aacaagatcc agaagaagct aactttctaa catcgttgca tcttcattta atgcttaatg
      661 agcaaaatat tgttttagct actcatccaa aatatttcaa aaatatataa cttttaactc
      721 tttttaaatt aaatggttgt tctgatcaga aaaatatcgc ttgttttctt attatgtaat
      781 atatttcata aactattctg cataacaact attgtttgta ctttttacat tggataacta
      841 attatgactg ctgataactt ttgatattaa actagtatct ccaatacaaa taaaaaatag
      901 atatcttgtt catcaaaatt agttcgtgga tacttacctt catttacatc attcagcggt
      961 ttcccatttt cttttattct attatttcat tttctaatag acttggcttc atttcgcaat
     1021 actttttttc aataggcatc aaattggtgt tgtttagata tttagttgta gttttgtgac
     1081 atgataagta tgctcctttt tctcttttcc actaatacaa cgatcaaagg gtgctttttt
     1141 caaaaaacta gaaaaatcaa aataagattc gtattttgat tctatatatc ctgtttcaac
     1201 tacatcttat atttcacaat agaataaata gccttttgca tagctgctta acttataaga
     1261 aattgcaatt tacttcttca aagtgaaaac gaatatgagt aaaataaaat tattcttctt
     1321 acagataatt aaaaatagcg cgtaacaaaa atcggcattc aatcacataa ttaggattga
     1381 tgccgattac ttatattcta cgtatagttt tagtattctt attttttatc accagtaacc
     1441 aattgagaac agccctaaag cagaatagtc ttttatctca aaacgcttaa tttcaggctt
     1501 aggaatatga ataatctttt gagtatattc tttctctttt aatcttactg aagaaatacc
     1561 actgcc
//
LOCUS       DAZKMB010000224         1547 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000224 DAZKMB010000000
VERSION     DAZKMB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.231"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            451..1491
                     /locus_tag="ACG8V3_002874"
     CDS             451..1491
                     /locus_tag="ACG8V3_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0301325.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          601 a          203 c          244 g          499 t
ORIGIN      
        1 atatctgaca ttggtagtac ttattgtatt ttttagtagt atataatcta ttgttttcta
       61 gcctaaagcc ttgtcttata agaataatat aatatatact actgaaataa ggtagtatat
      121 attactagta atggtagtat gttttatatg cataaggtag ttcttaattt attttttgtt
      181 ttgacactgg tagtacttaa ttatctttaa ttttaaaact aaattttatt catacgtttt
      241 tctggtagta tttaattata aataatattt atttaaagta gttcttaatg actttaaagg
      301 tagtacataa ttcattttat ttctgatttt aggtagtatt taattaaaaa acagttttaa
      361 tgcgtttgca ttttttattt atctttgcta ttttcgaaaa gagtaggtaa ttttacataa
      421 ataaaacaac gtgaaagtag aggatctaca atgactgatt ttaaattttt taaggcggat
      481 agagtttaca acgaattatt ttatcaattt ccaaaagtct ttattgtttc tgacgaatac
      541 aaaaaaatga aagattcaac taagattgcc tatatgcttt taaaagcaag attagagatc
      601 gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt attttactta tacgacaaat
      661 gaactatgta gagtattaaa ctgccaaaaa caaaaagcga tagcaatcaa aaaagagttg
      721 gaatcctttg gtttattatt acaaaagcag atgggattta acaaacagtt agggaaaaat
      781 aatcctaata gactatatct agcagaatta aaagtctcag aaaatgatat ctacttactc
      841 gaaaaatttg atagagagaa tagggaaaac gttgataaat cagagggtat gaaaatcata
      901 cccaccctcg acgaaaaatc agacgctgaa tcccttgggg ctcaagaggg tatgaaaatc
      961 ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa tcataccaga acttaataat
     1021 aatatattag acactaatag acacaatata gacactgaaa aagaccgcct acaagatcaa
     1081 ttgttgttag acaattttga gacaattatg acaaacgaca gcattgctac gtttgtccct
     1141 gaacgagtat taaatttgat aaaaacattt tcttcaagtt acagtgaagc tcaaaaaacc
     1201 gtccagacta ttcataatgc aaagaaaaaa gctgaaatag aaagtgatat ttcgatagtt
     1261 tttgaagaac tcgatagtta ttatgtcaat gcagaacaag aattatacac gacactgtta
     1321 aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata tccagaacct gatttttgtc
     1381 tatgtaaaaa attggtttat cgaaaaacca atagctgcta aagtatcaag tgaaaaacgt
     1441 ttgaattatg caagctcccc aagcactatt acgaaagact ggttagagtg aaactttact
     1501 gacaaagtga ttgttgcttc atggagtaac attaaaatta aaaattt
//
LOCUS       DAZKMB010000225         1536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000225 DAZKMB010000000
VERSION     DAZKMB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.232"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..1491
                     /locus_tag="ACG8V3_002875"
     CDS             25..1491
                     /locus_tag="ACG8V3_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0301326.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          423 a          289 c          326 g          498 t
ORIGIN      
        1 atttaaagaa ttggggtata gattatggaa aaacagcaag acaagggctt cttcgggcag
       61 ccgaagggat tgtcaacttt gttcttcact gagatgtggg aacgatttag ttattacggt
      121 atgcgtgcat tacttatcta ttatatgtac gatacagttg ccaacggggg acttggatta
      181 ccacgagcaa cagcattagc tatcatgtcg atctacggat ctttgatcta catgtctagt
      241 atcgtcggcg gatgggtatc tgaccgagtg ttaggagcaa aacgcactgt attcttcggt
      301 ggagttttca ttatgcttgg ccacatcgtc ttggctttac catttggtgt aagtacattg
      361 tttgtttcga tggcattgat tattatcgga accgcctttt tgaaaccaaa tgtttctggc
      421 atggtcggac atctttattc aaagactgac ttgcgaagag atgcaggatt ttcgattttt
      481 tacatgggtg tcaacttagg ggcattatta gcaccactaa tcgttggtac aatcggtcaa
      541 gaatataact atcatttagg gttctcttta gctgctattg ggatgttctt tggattatta
      601 caatacatca tccaaggacg caagacatta gacggagtgg gtacaacacc gccgaatcct
      661 ttgacaactg aagaacgtaa aggattcatc cgtaacttga tcatcgcact tgttgttatc
      721 gctgcagtat tcggcggggc acaactaact ggtcatctga caattgactt ttttgtcaat
      781 acaatcagtg tattggggat tatcttaccg ttgtattact ttatcaaaat gctgacttca
      841 aaagatgtaa ctgctgaaga aaaaccaaga gtatgggctt atatcccatt attccttgca
      901 gcaatcattt tctggtcatt acaagaacaa ggttcatcta tcttagcttt atttgctagt
      961 gaacgtacac aatctacgct atttggtgta acgatccaag caagctggta tcaatcattg
     1021 aatccaattt tcatctttat tctgacacca gtattcgtaa cgatgtggac gaaacttggt
     1081 gaacgtcaac catctacagt agtgaaattc tctcttggac ttttatttgc aggattatcg
     1141 tacttgctat tgatgttgcc gggtatgctt tatggtacaa gtggaaaagt aagtccattg
     1201 tggttgttgg gaagtttctt catcgtggtt attgctgaat tgtgtctttc accagttggt
     1261 ttgtctgtga caacaaaatt agcaccacga gcatttgaat cacaaacaat cgctatttgg
     1321 ttcttagctg atgcttcttc acaagcaatc aatgcacaaa ttgcacgctt ctacacaccg
     1381 ggaacagaat ctgcttactt cggtattatc ggtgcagtag caatcgtcgg tggtatcttg
     1441 ctatttactg tgaaagaacc aatcaaaaaa ctaatgggct ctattcatta atcaatgaac
     1501 ataaaaacag gaattgctta tttgcagcag ttcctg
//
LOCUS       DAZKMB010000226         1536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000226 DAZKMB010000000
VERSION     DAZKMB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.233"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG8V3_002876"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG8V3_002876"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZKMB010000227         1508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000227 DAZKMB010000000
VERSION     DAZKMB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.234"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1214
                     /locus_tag="ACG8V3_002877"
     CDS             <1..1214
                     /locus_tag="ACG8V3_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0301327.1"
                     /translation="VPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPE
                     GTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQ
                     KVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEV
                     EVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVS
                     TAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEV
                     TVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKD
                     SGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
     gene            1348..>1508
                     /locus_tag="ACG8V3_002878"
     CDS             1348..>1508
                     /locus_tag="ACG8V3_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0301328.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEV"
BASE COUNT          601 a          216 c          320 g          371 t
ORIGIN      
        1 aagttccagt agaagtgaca gacaatcgtt ctgacgccga taaatatgaa ccaacagtag
       61 aaggcgaaaa agtagaaatt ggaggcacag tagatttaac agataatgtt actaacttgc
      121 cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca attgatacca
      181 acacaccagg caattatgaa ggtgtcattg aagtaacgta tccagacggt acgaaagata
      241 cggtgaaagt tccagtagaa gtgacagaca atcgttctaa tgcagataaa tacaatcctg
      301 aaggacaaaa agtgactaca gacttaaata aagagccaga tgcatctgag ggaattaaga
      361 acaaagaaga tttaccaaaa ggaactactt atgcttggaa agagaaagta gatgttagca
      421 cagctggaaa taaaaaaggt actattgtag tgacatatcc agatggatca tctgatgaag
      481 ttgaagtaga cgttacagtg acagatactc gttccgatgc agataaatat aatcctgaag
      541 gacaaaaagt gactacagac ttaaataaag agccagatgc atctgaggga attaagaaca
      601 aagaagatct accaaaagga actacttata cttggaaaga aaaagtagat gttagtacag
      661 ctggaaacaa aaaaggtact gtagtagtaa catatccaga tggatctaaa gaagaagtag
      721 aagttactat ttctgtagta gataaaaaag caccgaataa acctcaagtt gatcctatta
      781 cagagggtga ccaaattgtt actggtaaaa ctgaaccaaa tgcagaggtg acagttacat
      841 taccagacgg aagtcaacac catgggacag ctgataaaaa tggtaatttt acagttaaag
      901 ttcctaaatt agaggcaggg acaaaagtga tagtaactgc aactgatgaa tctggtaata
      961 ctagtgaacc tactaatgta gttgtttctt ccaacaaaaa agacagtgga aaaaacggta
     1021 gtaaaggtag taagactgac aatcaaggta gcaacagcaa tcaagacaaa aatagaggca
     1081 aaagccagtc ctcaaaagtt tttcctaaga cgggagagag tgacagtaat atctttacta
     1141 ttagcggtgg tttaatactg ttaggaacgt taggattatt agggtataaa aatcgaaaaa
     1201 aagagaatga atagaaaatc aatttcttaa gtcttctctg agaagaggaa gaatcttttt
     1261 cttcctcttt ttgctttttt atgatgggaa ataagtgtta gttaaagatg aatatacttt
     1321 ttatagaaag gaaaagaatg taattaagtg gaaaaaatag taattattgg ttcaaaccat
     1381 tcagggctag ccgcagctcg atttctaaac ttaagcgatt caaattatga agttattcta
     1441 atcgataaaa aatcagaaat tagttacttg gaatgtggga catcgttatt tataaaagga
     1501 gaagtaag
//
LOCUS       DAZKMB010000228         1482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000228 DAZKMB010000000
VERSION     DAZKMB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.235"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACG8V3_002879"
     CDS             <1..828
                     /locus_tag="ACG8V3_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0301329.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETKIVDA
                     KKGKINYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFERWRQEQEHSFQDWREGQESDYLAWF
                     ESIKDILKTIDPGGVMLAELTDARVDIQGVRHASISERLLADMDYLYQKLRATLFTIE
                     YGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1448
                     /locus_tag="ACG8V3_002880"
     CDS             828..1448
                     /locus_tag="ACG8V3_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301330.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          529 a          212 c          337 g          404 t
ORIGIN      
        1 aatacagagg ttatggaagc gactatagtt gaaaatggtc ttccctacga tttatcagaa
       61 tgtaaggtat attttgaatc agttgtaggt gggaaatatc cagtccaatt agaaacaaaa
      121 attgtggatg ctaaaaaagg gaaaattaac tatatttttg ataaatattc catgcagtgt
      181 ttacatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cttattttgt cattaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caatcgaaga tttaattgcg gatatgacgg actttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa
      421 caggaacaca gtttccaaga ttggagagaa ggacaagaat cagactattt ggcatggttt
      481 gaatcaatca aggatatttt aaagacgatt gatccaggtg gagtcatgtt agccgaacta
      541 acggatgcgc gagtagatat tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg
      601 gcagatatgg actatttata tcagaaattg cgagcaacgc ttttcacgat tgaatacggg
      661 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca tgaagtcgaa
      721 aaagttggaa ctgtagcatt cccgattgaa gaaggagcct tgatcatcgc aaccgttgat
      781 gatccaaagc aaaatgtgtt cactcttgag aaagttgggg tgatctaatg gctaaaacta
      841 aacgaatgat ggaaacggat gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac
      901 atgcttccgc agttcttggg ttagaagaaa taatagcagg agaagcaaca gttttatctg
      961 ttaatggaaa aattggagcc gtcgtcatta ctaaagaaga tttgggatta gagaatgttc
     1021 tcacagaatt accctatgca agtgaagaag atgacggcat tatcacggca gaaatgtatc
     1081 aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc agttgccact atcgaccgtt
     1141 tgggtggcat aaaagttggt gaattattga cgattgatga gacagggaaa gtctctgcag
     1201 tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa
     1261 attatactgc tggagaaaac atcactattg atgaagatgg aacaattagt tctacaggtg
     1321 gtagtgggac tggtggtgtc aatcagtcat atgtagatca aaaattccaa gaagctgtaa
     1381 atcaagcaga aaattacaca aacgaaagaa ttccaaattt tacttttgaa aaaattgggg
     1441 aggtatagag aatgacagat atcgtaaaaa taaaacaaag ca
//
LOCUS       DAZKMB010000229         1473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000229 DAZKMB010000000
VERSION     DAZKMB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.236"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACG8V3_002881"
     CDS             5..403
                     /locus_tag="ACG8V3_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0301331.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="ACG8V3_002882"
     CDS             387..1082
                     /locus_tag="ACG8V3_002882"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0301332.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          557 a          213 c          296 g          407 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atg
//
LOCUS       DAZKMB010000230         1459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000230 DAZKMB010000000
VERSION     DAZKMB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.237"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8V3_002883"
     CDS             131..505
                     /locus_tag="ACG8V3_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0301333.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8V3_002884"
     CDS             complement(619..1248)
                     /locus_tag="ACG8V3_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0301334.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8V3_002885"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8V3_002885"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          489 a          223 c          258 g          489 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattat
//
LOCUS       DAZKMB010000231         1450 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000231 DAZKMB010000000
VERSION     DAZKMB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.238"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..144)
                     /locus_tag="ACG8V3_002886"
                     /pseudo
     CDS             complement(1..144)
                     /locus_tag="ACG8V3_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(186..1312)
                     /locus_tag="ACG8V3_002887"
     CDS             complement(join(186..1084,1084..1312))
                     /locus_tag="ACG8V3_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0301335.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          421 a          259 c          237 g          533 t
ORIGIN      
        1 ctccagataa gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc
       61 ccaacgaatg atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta
      121 cagaagagtt cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga
      181 gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga
      241 atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga
      301 tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca
      361 tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt
      421 attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca
      481 gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga
      541 atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt
      601 aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg
      661 tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct
      721 tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt
      781 aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt
      841 aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt
      901 gaaatcttct ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta
      961 taaaaataat agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa
     1021 tccttacacc atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg
     1081 ctgcggcttt ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt
     1141 cagctacttc ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt
     1201 tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag
     1261 tttgattcag ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc
     1321 atcctctcgt agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga
     1381 tactaacacc aagttatctt tagaaccatt tcagatacat atgaaacaat tacatatgat
     1441 tttttcataa
//
LOCUS       DAZKMB010000232         1421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000232 DAZKMB010000000
VERSION     DAZKMB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.239"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..449
                     /locus_tag="ACG8V3_002888"
     CDS             186..449
                     /locus_tag="ACG8V3_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0301336.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            449..721
                     /locus_tag="ACG8V3_002889"
     CDS             449..721
                     /locus_tag="ACG8V3_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0301337.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            798..1127
                     /locus_tag="ACG8V3_002890"
     CDS             798..1127
                     /locus_tag="ACG8V3_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0301338.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1117..1404
                     /locus_tag="ACG8V3_002891"
     CDS             1117..1404
                     /locus_tag="ACG8V3_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0301339.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          534 a          174 c          281 g          432 t
ORIGIN      
        1 ttgttgattt accaacatgt ttagacattt tcctctagga aaaaattaac tagcaccacg
       61 ggtataaagg aaacggataa cactttacaa tgctgttttg gtactccaat ttaaacaaaa
      121 caatcgaatt tacgtactta attaagtacg ttagaatgga gatataaatt agagggggag
      181 tagatatggc aaaaacagtc ttaaatccta gctcagcacg gaaaaatttt tataagttgt
      241 tgaaagatgt aaatgagaat catacggaaa ttgaaatcat tagtgagcgc agtgaaaata
      301 atgcggtgct tattggtttg gatgattgga aagcaatcaa agaaacgcta ttacttgagc
      361 aaataggcac tttgggtgtt gttcgtaagc gagaaaaaga tgatagtgga tttactaatg
      421 ttgatgaaat tgattgggat aacttataat gaataactat tctgtgatga ttaaaaactc
      481 ggctaaagct gatttaaaga aaatcaaaca gtctaatttg aaacctcaat ttgagaaggt
      541 tattcagact ttaaaggaag atccctatct ccctacccaa tcatttgaga agttaaaacc
      601 tacacatgaa ggtaggtatt ctagaagatt gaatagacaa caccgggttg tttataaggt
      661 agatgaagat gaacacgtgg ttgaaattta ttcagcaagg acgcattacg agtcacagta
      721 attacttgta aatacattat ctcgtggtat aatgtattta ctaagtaatt acatactgtt
      781 agaaatgagg gatttaaatg attacaaaaa caagaaaaca aggcaattca atcatgttga
      841 cagtacctaa agattttaat gttcctaatg gagtcgaagt tgaagcaaag ttggtagaga
      901 atggtattct ttatgaattt gttgaaccac aaaaagaatt ttatgatttt agcgaagata
      961 ttttgtctga tataattgca gaaggctacg ataaagatga gattcttgta gaatttaaga
     1021 atagaaaaaa caaaatgcat tcttccttta gagatattgc tgaagataca cttactaatt
     1081 ctaaagtaat gactaaagag gagttggcaa cagaaattgg cttatgaaat cgttccttca
     1141 aaacatgtca ttaagtattt aaaaaagcta aaagagaagc cattgaaaga aaaatttttg
     1201 aatttaattt atgatgaaat tgcgattgat ccatataagg gtgaacaaaa aactggggat
     1261 ttatcaggta tttggtctag tgggttcaaa tatgcgggca caacatatag agtagcttac
     1321 gagattatag aaaacaaggt tattccagta ttgttatgtg gaacgcatga aaacttttat
     1381 gaacagttga aaaaattaat ctagtgattt gttgtcgttc t
//
LOCUS       DAZKMB010000233         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000233 DAZKMB010000000
VERSION     DAZKMB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.240"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8V3_002892"
     CDS             complement(150..1337)
                     /locus_tag="ACG8V3_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0301340.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKMB010000234         1413 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000234 DAZKMB010000000
VERSION     DAZKMB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.241"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..224
                     /locus_tag="ACG8V3_002893"
     CDS             <1..224
                     /locus_tag="ACG8V3_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0301341.1"
                     /translation="VFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDSEE
                     DFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            229..1176
                     /locus_tag="ACG8V3_002894"
     CDS             229..1176
                     /locus_tag="ACG8V3_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0301342.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          465 a          195 c          294 g          459 t
ORIGIN      
        1 aagtttttga aatgaatgcc tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta
       61 attatatttt tgatgggcaa tgtgggataa taaaaatgag agattatcta attttagata
      121 ttgatagtga agaagatttt aaatggttag aatttttgtt tcctaaattt ttagaagatt
      181 cttcggagct tcaagatatc tataattatt tatatcggag gtaaaaggat gagctgttat
      241 cttatcaccg gtggggctgg cttcattggt tctaccttag ccaatcattt aggagaagaa
      301 catgtggtaa tcgtcgtcga tgacttatca atgggaaaag tagaaaatct tgatatgacg
      361 aaaaatatca cttttattga gggagatgtc gctgagaaat ccttgatgga agagatcatg
      421 aaagctaata aatttgatta tatctttcat ttagcagcag ttgctagtgt ggcagattca
      481 gttgcccgac cagtagaaac acatcgtgtg aattttgaaa gtgtattaat gattttagaa
      541 ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt
      601 tatggggatg aaccgacttt accaaaaaaa gaagagtccg tgattcgccc attgacgcct
      661 tatgcggttg ataaattcgc tgcagaacaa tatgtgctag attattgtca tttatatgat
      721 gtacccggta gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa tccgaattct
      781 ccatacagtg gtgtgatttc tattctcgta gatcgctata agaaacaaat cgtaggagaa
      841 gcaacttcat ttacattatt tggtgatgga agccagtcta gagactttgt ttatatagat
      901 gatgtaatcc aagcattact tttagttgcg aatgaggaag ctgcactggg gaaacaattc
      961 aatgttggaa cgggtaaagc aaccacactt ttggaattga ttcatgcaat caatgaaatt
     1021 ctcggtgtag aattgacact tgaatacaaa gaagagcgtt cgggtgatat ccatgactct
     1081 ctagcagata ttactaagat ccagtcaatt ggctatcaac cgaaatatgg tattttaagt
     1141 ggaatgacta attatttgaa aactgaaatc aattaaaaca tactaagata agattagagg
     1201 gtaaattcag gctctttgtc aataaggact gatgagtttt gtaaaatcaa atctgggata
     1261 gaagggctct ttgtcaaatg atgttggtaa agagaaatct tagaataatt tggacagaac
     1321 agaaagaaga aaacctcttt ctgttctgtt tatttggtta aaaatgtttt gccatgacga
     1381 atcataattc gtagtttgaa attgtaaaag ttg
//
LOCUS       DAZKMB010000235         1393 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000235 DAZKMB010000000
VERSION     DAZKMB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.242"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            386..718
                     /locus_tag="ACG8V3_002895"
     CDS             386..718
                     /locus_tag="ACG8V3_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0301343.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            628..822
                     /locus_tag="ACG8V3_002896"
     CDS             628..822
                     /locus_tag="ACG8V3_002896"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0301344.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            861..1007
                     /locus_tag="ACG8V3_002897"
     CDS             861..1007
                     /locus_tag="ACG8V3_002897"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301345.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
BASE COUNT          492 a          246 c          245 g          410 t
ORIGIN      
        1 aaaacgaatg attttaatta tcaacgtttt ttaattcgtt tttagcttaa aaataacttt
       61 tgtcacagtc tcactcctaa aattacttta tttacgtatg aagtgggcag gaggtcgaat
      121 ccagaactgg gctatggtac ttcaacgagg acttctcctc tagaattgaa aagcatgcga
      181 tttaccttcc gtaacgacgg tagatttatt caactatgtg tccaatcaaa atttgtctga
      241 tttcaaaaat agtcaagaga attttggttg ttaaaaactc ttatctatca acttccacag
      301 tttagttgac aaaccctgat actaagttgg gctatgatac acttttcttg actatcagtt
      361 acgttaccgg aaggaatgaa ctctattgag aaaaacatat gatcgtaaat ttaaactaaa
      421 gatttcacag gatatccttg agaaaaaagt aactaccaag aaaattgctg aagaatacaa
      481 tatctctcgt ccaacaattt cccgatgggt ttcagaatat cgtcgatacg ggaaaaatgc
      541 ttttgctgga caaggaaaaa gattacctga taaagcagat ttctacattc ttgaacagga
      601 aaataaacga ctaaaagagg aagacgagtg ttctacgaac acaaagatcg atacggatct
      661 ccagggatca ctcaaaaact ttacgatgaa ggaatcgaaa tcaataaacg tgttgtagct
      721 gttcttatgc gtgaaatgaa tttatgcaca agaggatttc atcgacgcaa atcttctcat
      781 ggtagagaaa aaacaatcaa agaaatggta aaaaaatgtt gaatcgataa tttgaacaga
      841 accaaattga tgctgtttgg gtgatggata ttacttacat tccatgtaat gacggtcgcc
      901 tctatttgtc cacctatatt gacttagcaa ctcgtattct acgttactat atggtagatt
      961 cacacatgaa aaaagagatt gtcgtccgtc cattacaaat ttattaagga gaattgccta
     1021 cggtaattca ttccgataga ggtagccagc atcggtcttt tatttatcaa gagctgttga
     1081 acgaccacca catcacacat tcagtgagcc aaactgggac tcctgttgac aatgcagtaa
     1141 ttgaagcgtt tcatcgatca atcaaatgag aattaatcga ctccaacaaa tataaaagta
     1201 aaatagagat gaaggtgtta atccaagatt atttagaaga ctactaccca aacaaaagaa
     1261 tccatacaaa atttatgatg actccttgac aatttgaagc gaagaaaata agggatctat
     1321 aatatctagt gttggtgacg ccttctgttg ccaatacttt ttcttttttc taataaacgc
     1381 aaacagacac ctt
//
LOCUS       DAZKMB010000236         1364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000236 DAZKMB010000000
VERSION     DAZKMB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.243"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACG8V3_002898"
     CDS             82..>1364
                     /locus_tag="ACG8V3_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0301346.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAZKMB010000237         1352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000237 DAZKMB010000000
VERSION     DAZKMB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.244"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG8V3_002899"
     CDS             <1..459
                     /locus_tag="ACG8V3_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301347.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG8V3_002900"
     CDS             473..904
                     /locus_tag="ACG8V3_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301348.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..1043
                     /locus_tag="ACG8V3_002901"
     CDS             906..1043
                     /locus_tag="ACG8V3_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0301349.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            1081..>1352
                     /locus_tag="ACG8V3_002902"
     CDS             1081..>1352
                     /locus_tag="ACG8V3_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301350.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          506 a          196 c          278 g          372 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga agaagcagat aaaatcatta
     1021 gcaaagaaga gagcgcatct taattgatgt gctttttatt ttgattcaag gagttgtcac
     1081 atgattaatt taggggaatg gggagcgata gcaggatcaa taaccgctat cgtttctttg
     1141 attttattag taataaaacc aattactgca tctttctcga agattactga gactctttca
     1201 aaagtaagtc acaatttaga tttgctgact aaagatttag aatcgagcaa atcagatcga
     1261 ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca tacagaaaaa
     1321 ttggtggaac acacgcaaca aattaaaact tt
//
LOCUS       DAZKMB010000238         1344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000238 DAZKMB010000000
VERSION     DAZKMB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.245"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACG8V3_002903"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACG8V3_002903"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0301351.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          461 a          180 c          224 g          479 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaa
//
LOCUS       DAZKMB010000239         1294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000239 DAZKMB010000000
VERSION     DAZKMB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.246"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG8V3_002904"
     CDS             <1..1244
                     /locus_tag="ACG8V3_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0301352.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZKMB010000240         1266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000240 DAZKMB010000000
VERSION     DAZKMB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.247"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..689)
                     /locus_tag="ACG8V3_002905"
     CDS             complement(21..689)
                     /locus_tag="ACG8V3_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0301353.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            823..1110
                     /locus_tag="ACG8V3_002906"
     CDS             823..1110
                     /locus_tag="ACG8V3_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301354.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT          472 a          214 c          214 g          366 t
ORIGIN      
        1 aaagtagtga aaaataagaa tcagtgctct aactgttcaa gaggatcaag tggttcaaat
       61 gtttctgtag tctgttttgc ttccaattgg ggcaaaacag atccaatttc cagatacttc
      121 actttattca atgtataaga tagaatagaa cagctaagta atgtatacag aaaatgtgtg
      181 agatccaaat aaagcaagga gacaactaaa ataccaaaag taatcaagaa aagacttttg
      241 ccaatcttaa tatgaaaata tttttcaatg gcttgataaa tgatatctgt tccgcctgtg
      301 cttccgccga atcgaaagac gagaccgcaa ccaatgccag aaagaatacc tgtcatcaaa
      361 ctattgatca gtagactatc tacagcaaaa tgtatatgaa ggctctccca taaatgtaaa
      421 aagaaaacta gcgcaaatgt tccgacgatc gtccggagaa aagcgtgttt tccaaaaaga
      481 aacaatgaga taagcaataa aggtaaatta aaaagcagtg aagtgacaga aggagcgaat
      541 ccaaaaagat tatgcaaaag caaagcaata ccagtaaccc caccatctgc cagcttagat
      601 ggaatattga ttcctgtcac agctaatccg tagattcctg cgcctaaaat gattccgcac
      661 aattctgtaa ataaatttaa ttttttcatt tctagcctct tttcttttga aaattttcag
      721 caacttcttt attatactga cttatactct tacttcaaga ggaaaatgaa aaaaaattat
      781 ttttggctgt ttctttttat ttatcaaata aggagtggaa aaatgcgagc agcagactta
      841 ttgacaattc ttacaaaatc acccaaaaaa acagagtatc aaggagtttt tttggctcaa
      901 gaacaaaaac tggtgcctgt gacaatggtc cagataaata aagcaaatca atttatcttt
      961 ttatttgaac acggcaaagc accacttgct atgaaagaca tctttctttt tttgatgaag
     1021 aaccgtgaga aagctgttta ttatcagtca aaagaagaac tcattcctct ttatggagtg
     1081 aaagagatag aaaataaagt ggtgatataa gtaaaaataa gaaaacaaga cgtttatgat
     1141 gtcaaaacaa ccatttttta catttttggc aaaaaagtgt catgatacag tataatattc
     1201 gttgaacagg ccaataaagg tgttctcccc cacgagattt ctactttatt ggctgtttct
     1261 ttttat
//
LOCUS       DAZKMB010000241         1260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000241 DAZKMB010000000
VERSION     DAZKMB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.248"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(173..1030)
                     /locus_tag="ACG8V3_002907"
     CDS             complement(173..1030)
                     /locus_tag="ACG8V3_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0301355.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          358 a          253 c          184 g          465 t
ORIGIN      
        1 tctgtctttc aacattggag gaagttttta tttcttgaac ttggtgcact tctaattgct
       61 tattttcaga tggtgtaatt gcttgctgct ttttctttgc ttctgcttct ttttttattt
      121 tggcgagtcc tttgttgttc ccaaaaatat caagtccttt gatgtcagcc aattactcca
      181 cctccgcata ccaattctcg tgttcttcca attctttcaa aatactgata aataattcgt
      241 ggcttttgcg atcccattgt tttaagagac ctttctttgt cttctccatc gtaatacctg
      301 atccgtcgta tcgttttaag cgcgcatttt tgagtacaac atttttaatc aaatgttccc
      361 cataaatctc ttgggcttgt ttatacattt cctgatctac tgcgtcaccg ctttccaaca
      421 tgaaaggaat aacaccagca acatataaat ttgtatcaaa ttctttcaca aaaaagtcca
      481 tatactcaag atattgcatc gcatttctca tcgattttac ttgggtctgt aagacgacga
      541 ttgcccagtc cgcaatgtac atcgcggatt tactatatac agaaatagaa ggtgggacat
      601 ctaaatagat gacgtcaaac tcatcaaata atggttctag cttttctttc atatatttga
      661 attgggataa tgaatcatta tattggtctt tgatccaatc atcatacatt tcaaaggaca
      721 tgtctgctgg aagtatatag aggttattga ttactttata gacagcctct ttaggtggaa
      781 gattgtttat caacacgtca gtcacagtat tttcgtaaac aatcggttca ccttctccgc
      841 ctccttcttc gttagtgaag ttcatatgcg ttaagttcat cacatcagaa gtatttcctt
      901 gcatatccaa gtcaattatc aaggttcttt ttccttcatt aactgctgat tcaaacccta
      961 acatttgcgc gcacgtactt ttacctacgc cacctttaaa attaccgatt acatatttct
     1021 tcgctaccac gttcctacct cttttctttt agcaatattc atttgatact attataacat
     1081 atttattttt agttactacc aaaaaaaaga tatatatgta ctaccttata attaataagt
     1141 aaaaggtagt acataaataa ggaaaagctg aaatatattg atttatagct ttcctcaaag
     1201 taatgacgtt atttaattta tcgagaagca gagaatactc gcgactttag tcatgtagac
//
LOCUS       DAZKMB010000242         1257 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000242 DAZKMB010000000
VERSION     DAZKMB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.249"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1192
                     /locus_tag="ACG8V3_002908"
     CDS             <1..1192
                     /locus_tag="ACG8V3_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0301356.1"
                     /translation="KGVQAQFIKGRLDDSPSHCEHCGSIRIIRNGSYTTRTQMLKVKE
                     KLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKK
                     YFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTNQLF
                     TILEDRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIV
                     QHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFK
                     RPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRDTEGFQETLKTISSTLPQSFKK
                     KFTIFHRYQSGISNAFKVPHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGL
                     IFEN"
BASE COUNT          385 a          273 c          247 g          352 t
ORIGIN      
        1 taaaggcgtt caagcgcagt ttattaaagg gagattggat gactcacctt cccattgtga
       61 acactgtgga tcgattcgta ttatccgcaa cggttcctat acgacacgca ctcagatgtt
      121 gaaagtcaaa gaaaagttga ctattttaga attaaaacgt acccgttttc tctgccatga
      181 ttgtggacgg accttttctg cgaaaacgga cttagttgat gaacatcatc aactaacaaa
      241 ggaactgaag caggcgattc tcatggaact gtacgaaaat caatcacgta aactaatcgc
      301 taagaagtac ttcgtctcgg atgggacggt gacacggatc ttgcgtgaag caacgaagca
      361 ttaccagcca cgtatgaact tccttccgac ggtgttgtgt atggatgagt tcaaatcaat
      421 gaagtctgtt tctggttcca tgagttttat ttgtgttgat gggacaacca atcagctatt
      481 tacgatcctt gaagatcgca gactttacaa actaactcaa tacttcctgc gctttcctag
      541 aaaggcccgt ttaaaggtca agtacctagt gatggacatg aatgccagct attgtcagtt
      601 gcttaaaaca gtctttccaa atgcggagat cgtaactgac cgctttcata ttgtccaaca
      661 catcaatcgc agcttcaatc aattacgtgt ccagatcatg aatcgttttc gaacctcaca
      721 ctctgaaaat cagaagaagt accgtcggct caagcgatat tggaaactct tactcaaaga
      781 ttccacgaca ttggagccac tcaaacggca ttatcatcga ctgtttaaac gaccaattag
      841 tcaaacagaa atcgttgatg agctgttaag ctacaacgaa gaattgcgaa ctgcgtatca
      901 tttttgtcaa ttattacgct attatttcgt taaacgagat acggaaggct ttcaagaaac
      961 ccttaaaaca atttcatcta cgttacctca gtcgtttaaa aagaaattca cgatcttcca
     1021 tcgctaccaa tcgggtattt caaatgcctt taaggtgccc cactcaaacg gcgtcactga
     1081 ggggctgaat aacaagatca agctgattaa acgaattgcg tttggttatc gcaactttta
     1141 taactttcgg gcacggatct atttacaaca aggtctgatc tttgaaaact aaaaaaatat
     1201 gcggcgcaca gaacgcgccg catatcggac taagttttat tcaaatttgt cttcgcc
//
LOCUS       DAZKMB010000243         1249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000243 DAZKMB010000000
VERSION     DAZKMB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.250"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..607)
                     /locus_tag="ACG8V3_002909"
     CDS             complement(<1..607)
                     /locus_tag="ACG8V3_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0301357.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINS"
     gene            complement(607..>1249)
                     /locus_tag="ACG8V3_002910"
     CDS             complement(607..>1249)
                     /locus_tag="ACG8V3_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0301358.1"
                     /translation="LYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWED
                     YRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIE
                     HETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDE
                     VMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          428 a          212 c          141 g          468 t
ORIGIN      
        1 tactgttaat ttcaagattc gaatatttac caaactgttt ttttactgat ttagggaaac
       61 caaataggtt tataatataa tccaattcat gtatacagtc taattctact cctcccccca
      121 tagatttaaa ggcagaataa ttatttctat aatcagtacc ttttctccaa tcaggaagat
      181 aagtagaaca aatagctctt acagaatata cttttttact ttgaatacta ttacgaacaa
      241 cttccatgat atttttatac ctcagtggag cagcaacata aatatttttg taatcataat
      301 tatattgagt aagatcaata ctctcagaaa aagcaggttt ttctaaaaat atgtaatcaa
      361 ctttttcaac aattacattt agtgtttcca gatgcttaaa agtcggattt gtaataaaag
      421 ctatatcata atgtgtatcc aaatcgtctc tattttgaat atacgttatt tcttcaaact
      481 ctttttttcc actgctacta taagctgtta tatcaaaatt aatagaattc ttttttccat
      541 agcttattag attttttaga tgtctctttc caattgaccc taacccaaaa aaaattactt
      601 tcatcattaa tttacctcaa tgtcgttaat taagttaata atatacttat ctttttcaaa
      661 agtatattgg ggttccattt tcttttccaa aagcgtgaaa aattctttaa tctcctctaa
      721 atatgcatct tcaataatag tatgtgaata attattatca tgcataacct catcgtacaa
      781 ttgaatatct gtcatactag aagtttcttc attccatgaa aatagagaat ccgggttacc
      841 ttcccattct attaaaatct tttcaccaat tatagttaaa tttcttttag ctactcttga
      901 aacaacatct acacttaaag ttccaactat accagtttca tgctcaataa taagagtgaa
      961 actgtccgaa taatcaatat caagattgga taatttctgg gtcattacag taaacgattt
     1021 aactttccca aatgtatcta taatccaagg taattcaata gcgaatattt ctctacaccc
     1081 attagttttt ttgtttgaaa caaaaaaatt gcgataatct tcccaaggat gccagtcggg
     1141 taaatactgt cctacatggt attgataaga aattttttta ttatgcgtca ctttagactt
     1201 aatatattga atttcttttc tcttcaaaaa tgtagaagat agataaagt
//
LOCUS       DAZKMB010000244         1241 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000244 DAZKMB010000000
VERSION     DAZKMB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.251"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            394..984
                     /locus_tag="ACG8V3_002911"
     CDS             394..984
                     /locus_tag="ACG8V3_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFQ0301359.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            1011..>1241
                     /locus_tag="ACG8V3_002912"
     CDS             1011..>1241
                     /locus_tag="ACG8V3_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0301360.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVS"
BASE COUNT          448 a          137 c          248 g          408 t
ORIGIN      
        1 tttactaaac caatccatta ttggggttaa aacattagca tatttaagcg gaatgggtag
       61 aaataggaga ttttaatcat aaaaaactgt actatagtga atttttggag atttatattc
      121 cagttatata ttaatgattg tgatacttgt actattgtaa aaattcgtta ggtttaaata
      181 actggaaata tcggaaaatg ggcattatac atcacggaca tcccttcttt tggaaaaaat
      241 gttacgattt ttataagtaa tattgtatcc ggtggaatca acgatgaaga taacatatcc
      301 atcgttgtag attcagtagc attgaatgat gctcttgttg caagaaatcc gaataaaatt
      361 gttaagtata aattgaatta ataggagata tgaatggaaa ttagcagtaa agaatattta
      421 aaagcggttc ttcgtatgga aaagaatcta tatatagaat ctaaaggaca acacacaaaa
      481 ttactaaaaa taatgaaatc acatccggac tacaacgctt ggcggtatac tcgtcgaatg
      541 agaattacat cttattatta ttcaaagaga aagaaaaata tcatttatgc gattatgtat
      601 attgtgtctt gcagaagaat gaatagtttg ggaagaaaat gtggtataga gtgtggtgaa
      661 aatgtatttg atgaaggtgt aagattttac catacacaag gaattgttat taatggtaac
      721 gccataatag gaaaaaattg ttacttatat gggaataact gtattggaaa tgatggaatc
      781 gatcctaagt gtcctgtaat tggaaataat gtacgtgtat gcgtgggtgc aaagattatt
      841 ggtaatgtaa aaatagctaa caatgtagtt gttgcggcag gagctattgt tatcaaagat
      901 tgtctagaga atggtgcaat tcttgcaggt gtaccggcaa aaataatagg gtatgttaat
      961 ttaacaaaag tatttttaga ataattttta gcgaaagaag gtgaataacg atgcgtattt
     1021 caataaaaag atggtcagga gcagattgga cattgttcac gttggcattt tttgcaatct
     1081 ccatagttct tgtatatact tttaacgccc caagagcaat tttatatttg ggagatataa
     1141 ttaatatctt tgtgttatta aatgcactta gctttagacg cagaaaagga atatatgcta
     1201 gcaaaaatga gatgctgata gttttgtttt tactagtttc t
//
LOCUS       DAZKMB010000245         1227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000245 DAZKMB010000000
VERSION     DAZKMB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.252"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            426..959
                     /locus_tag="ACG8V3_002913"
     CDS             426..959
                     /locus_tag="ACG8V3_002913"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0301361.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          390 a          227 c          230 g          380 t
ORIGIN      
        1 cgtttggttg gacttattac cgcagatgca agcttttgaa caccgtttat tgagaagttg
       61 tgaaatcagc gtcctgacct gagtattaga gaaacaaatg gcaaaatagt tcctcatatt
      121 ttgctatttt ttgagctaat accgagggtc agctgattga acaccgttta gtggagaagt
      181 tgtgaaaaag tcgcctactt tcgagtcgta agaaattttt ttgaaaatgg ttcctcacaa
      241 ctttgcataa atttgactta ataccgagaa gtcatgatcc ccatctaact ccaaatcaca
      301 aggaatgagt tcaaaaacta gcttttctca acaaatatta cataaaaaat tctatgtttt
      361 ttgttaaatt tatttgcttt tttcaaaagt atgttacagt aatttcccaa ggaggtttta
      421 cagaaatgaa ttttgttgcc atggattttg aaactgccaa tcatcagcct tatagtgctt
      481 gttctcttgc gctagtcatg gtaaaaaata gccagatcgt tgatgaattc tatacattga
      541 tacagcctga gactcctttt ttctggcgaa atgtccaaat tcacggaatc catcaagaag
      601 acgtacgcaa tgcgccgaaa ttcccagaag tctggcaaac tatccaacca tattttcaaa
      661 aaaatcgttt agtcgttgca cataatgctg cttttgatac aaaggtctta gcgggatgct
      721 tagattatta tcatcttgaa cagcctaatt atctttcttt atgtacagtg aaaagtagcc
      781 gtcgcctgtt tcctgaattt ccaaatcatc gattgaacac tgtgtgcgaa aatttgaaca
      841 tccaattaga tcatcaccat gatgcattgg aagatagtcg tgcatgtgcg gaaatcctgc
      901 tttatcaaga aaaaaaattc gggacagatc cattgaaaaa acttgtgact ataaagtaaa
      961 agaatactta tgaaacacac aaagaggtcg tgagcctgcc gtttcactcc gagtcacaag
     1021 gagcaatttc aaaaaatagc ttctcatgtt tattaaatat tgtgactggt gaccgaggag
     1081 ctggcaggag aacaccgttt attggagagg ttgtgaaaaa gctgttctgc atcaagtaat
     1141 aagaacagcc aaacaaaaat agcttctcat atttttcatt tggttggact tattaccgca
     1201 gatgcaagct tttggacacc gtttacc
//
LOCUS       DAZKMB010000246         1215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000246 DAZKMB010000000
VERSION     DAZKMB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.253"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..>1215)
                     /locus_tag="ACG8V3_002914"
     CDS             complement(552..>1215)
                     /locus_tag="ACG8V3_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0301362.1"
                     /translation="KVFKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIEL
                     AEVIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQ
                     ERIDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRIS
                     ELEKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKES
                     TV"
BASE COUNT          401 a          206 c          167 g          441 t
ORIGIN      
        1 ttaaagtcag ataaaaattc tttggaaatg taatggtaga gatattttta caaaaggact
       61 tgcagcgctg tagttggtgc tgcaggagga cctatttcgg ccgttacagg aggtattggt
      121 aatgtacttg tcggatgtgc attaaagcct gttcctgcaa gttaaattta taaaaacagg
      181 aaagtaatta tctcaaatcc tatcaagtac aatcatagga ttgcacttga caggacaaat
      241 tttttaacta aaaaatgaaa aacactttct aaaaattagt agaatttgcc aaatctttcg
      301 catcttccac catacattta attttaattc ccctttatgg tacttaataa aaacaagaaa
      361 agaactattc atttggccct tttttggttc cagaccatag aaatattcct ccagtaacta
      421 atattgctgt tatgcacgta actaataaca gctataaaaa ttggtacaac tttaatagga
      481 ttcagaaaaa tttgcactag tgaaggaatc tattttatta gaaaaatgca atctccctaa
      541 agaaaaaacc actaaacagt gctttctttc aacttctcct taattttttg tgctttccct
      601 aaagtaaagt ttctggccaa ctctgaatca aaatatagtg aacgtttttt aaaatctatt
      661 tctttaacgt taaagatatt tactaaacac gctcgactaa ttcttgttaa cataggatat
      721 ttcttttcaa gctcactaat tcttccataa aattcgtact gcccatcttt agtacacaat
      781 gataatctat gtggtaattt tgaagcttct acaaaataaa tgtcatttat atcaaaagta
      841 aatgtttgag aacctatgga aaaaacaaat gcttgattcg taacctgttt tgttgcatct
      901 atcctttctt gcgctaataa caataactca acaatctcgt ttcgatactc atctagtgtt
      961 tgatcttttg ttacaaatcc taacgtttca actcttcttt ttattgttac gggtaacatc
     1021 tcatcatgag tagttgtaaa aatgattttt gcttgaacat catattttct gattacttct
     1081 gctaattcta taccgttaac ttcatgattt aaatcaatat ctaagaaata tattccttgc
     1141 tttggtcgga attgtttgag gtatttttta acttctaacg gactttgtgt tttcaatact
     1201 atcttaaata cttta
//
LOCUS       DAZKMB010000247         1209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000247 DAZKMB010000000
VERSION     DAZKMB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.254"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..412
                     /locus_tag="ACG8V3_002915"
     CDS             2..412
                     /locus_tag="ACG8V3_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0301363.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            426..782
                     /locus_tag="ACG8V3_002916"
     CDS             426..782
                     /locus_tag="ACG8V3_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0301364.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          433 a          178 c          226 g          372 t
ORIGIN      
        1 aatgataaaa gtatatactt cgcctagctg tacttcctgt agaaaagcaa gagcatggtt
       61 gcaagcgaat caacttgaat ttgaagagaa gaatattttt gccgaaccgt taacagaaag
      121 tgagatcaaa aagatcttgg cagcgactga aggaggagtt caagatatta tttccactcg
      181 gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa ttaactttaa agcaaatgat
      241 cgtcttattc aaagaatatc cttcattatt gagacgacct attttgattg atgaaaaaag
      301 aatactaatc ggatttaatg aagatgagat ccggtcattt gtccctcgtg aaattcgatg
      361 tgttgccaga aatctgatct atttacaaaa caataacatc ttaaattcat agggagggag
      421 ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt aacaagtctg aaggcgaaac
      481 aatggttaga acaccatcaa ttgccttatc aatttgttga cttagaagaa cgcgatctaa
      541 gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa cagctatttg
      601 cgacttggtc tgaggctttt cacaacattg cgattgacct ttcctgtgta ggaaaaaaac
      661 aactgatttt tttatgcaca caacatacca atctcctcag acgtcctttg attattatcg
      721 atgaagttct atttattgga tacaatgagc agttgatgga acagcttatc ttaaataaat
      781 agactttaac tgttttaatc aagtcactga gcatttcata cagttgtaac agtcgaacac
      841 ttagcatcaa gcgtgttatg tgttcttttt ttgactttat ttttctacta tagagacatg
      901 ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa aatatattga
      961 catgtgtaaa tttacatgat atattcattt ctgttgtttt aagtagcaaa gcaataaaat
     1021 agtattttaa aaaacatata aaatagttgt tgacatgtta gaaatgacat gataagataa
     1081 ctgagttgtt aaaaaagaac cacttgtgaa gcttgattct atcgaaaaaa acttcaaaaa
     1141 agttcttgac aaataacaaa tgataagtta tgataaatga gttgctgtaa gatttcgaaa
     1201 aacgaaatc
//
LOCUS       DAZKMB010000248         1181 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000248 DAZKMB010000000
VERSION     DAZKMB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.255"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(199..1014)
                     /gene="thiD"
                     /locus_tag="ACG8V3_002917"
     CDS             complement(199..1014)
                     /gene="thiD"
                     /locus_tag="ACG8V3_002917"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0301365.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          311 a          256 c          196 g          418 t
ORIGIN      
        1 aaacatgaga agctgttttt tgaacttgtt ccttgtgact cggagctgga cggcaggctc
       61 acgacctctt tcttaaacgg tgttcaaaag cttgcatctg cggtaataag tccaaccaag
      121 ttaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcaactt
      181 tttcacaact tctttctttt actctaattc accagctgcc caatgattga tggggccata
      241 tttgtgccct acatttattt cttcacgaat tgcacggttt acatagtctt ttgccaatcg
      301 gatcgcttgt tcaactgttt ttcctttcgc taactcagct gcaatacatg cagatagcga
      361 atcgccagtt ccgttgatcc ggtcggtagg ataaaatggt gcatttaacc aaaatgattt
      421 tcctgattcg aacaatacgt agtcattgac tgtttcttgc tgcgcatttt tagcatgatg
      481 tcctttgatc ataacgtttt ttgcacccat atcttgcagt tcttttgctg cttgcacaaa
      541 atcttcttca gctgctaatg tccgtccagt cagcttttct gcttcataaa aattaggtgt
      601 cagtacttcg gccaaaggga caagcttttc tttcaagctt tgcaaagcag attcttctag
      661 taaaagattt ccatgtttcg ttacgattac gggatccacg actaatggtc caaaatcata
      721 tttttggtaa ttttttacaa ctgtctcaat caactttgaa tctgctagca tacctgtttt
      781 agctgcacgt atttgaaagt ccttcgcaat cagttcaaat tctttgtcga taaaatctgt
      841 tggcaatgtc acacttgctc cgattccgta agaattacct gcaacacatg cggtcatgac
      901 agaaacaccg taaacatttc ttgtaaagaa tgtatgcata tctgcttgca tccctgcgct
      961 tccatcgcta tctgatcctg cgatcgtcat tgcttgtaca tactgatctt ccatcaaatc
     1021 gattcctcca tttttataaa aagatttttc ataatactgt tactgtttct atcatatgtt
     1081 tttcaaagta tccctcagta aatataccac taccttttct tggtaatcaa atttatttga
     1141 aaaaacattt gcaataaatc agaaaatagt ggtcgtttat t
//
LOCUS       DAZKMB010000249         1165 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000249 DAZKMB010000000
VERSION     DAZKMB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.256"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..1157)
                     /locus_tag="ACG8V3_002918"
     CDS             complement(3..1157)
                     /locus_tag="ACG8V3_002918"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0301366.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT          321 a          253 c          219 g          372 t
ORIGIN      
        1 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
       61 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
      121 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
      181 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
      241 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
      301 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
      361 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
      421 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
      481 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
      541 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
      601 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
      661 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
      721 gccaagctgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
      781 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
      841 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
      901 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
      961 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     1021 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     1081 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     1141 tcaatcccta ttttcatttc aacac
//
LOCUS       DAZKMB010000250         1158 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000250 DAZKMB010000000
VERSION     DAZKMB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.257"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..879
                     /locus_tag="ACG8V3_002919"
     CDS             <1..879
                     /locus_tag="ACG8V3_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0301367.1"
                     /translation="AHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLV
                     LPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRK
                     PNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKV
                     IITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFA
                     DPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPL
                     EVFLSYVSIDDLSNLI"
BASE COUNT          398 a          178 c          217 g          365 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      781 aaacgaaata atattcctag aaaatcactg gattatcgaa cacctttgga agtatttttg
      841 agttacgtaa gtattgatga tctgtctaac ttaatttgac aattaagaca tttaaaaaga
      901 aaagtgacag ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt
      961 ctaatactag gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt
     1021 gtcagctatt ctatattaac attgattgct atggttttag gtacttcatt ttataatgct
     1081 tttgtaagta agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca
     1141 agtaattaat atgagaac
//
LOCUS       DAZKMB010000251         1158 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000251 DAZKMB010000000
VERSION     DAZKMB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.258"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..208)
                     /locus_tag="ACG8V3_002920"
     CDS             complement(<1..208)
                     /locus_tag="ACG8V3_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301368.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
     gene            complement(305..757)
                     /locus_tag="ACG8V3_002921"
     CDS             complement(305..757)
                     /locus_tag="ACG8V3_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301369.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(764..>1158)
                     /locus_tag="ACG8V3_002922"
     CDS             complement(764..>1158)
                     /locus_tag="ACG8V3_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0301370.1"
                     /translation="FDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIV
                     PRMEKVDWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQ
                     LGKAAAKELAKVNIVAGIAAAAASCALE"
BASE COUNT          412 a          228 c          121 g          397 t
ORIGIN      
        1 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
       61 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
      121 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
      181 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
      241 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
      301 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
      361 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
      421 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
      481 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
      541 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
      601 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
      661 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
      721 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
      781 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
      841 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
      901 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
      961 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
     1021 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
     1081 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
     1141 aaccaactgt atcaaaat
//
LOCUS       DAZKMB010000252         1113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000252 DAZKMB010000000
VERSION     DAZKMB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.260"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..851
                     /locus_tag="ACG8V3_002923"
     CDS             159..851
                     /locus_tag="ACG8V3_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0301371.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          487 a          153 c          185 g          288 t
ORIGIN      
        1 tataaaaaag attttttaaa tagataaaaa aggaggatta aatgagtatt tgatagtatt
       61 tatattaaaa ttacatatgt aatttaaaaa cgcttgacat tgtgtttgag atcacttact
      121 tttagcatgt aaaagtattg gattaaggag ctatgcaaat gaaaaagaaa tttattactg
      181 gtgggattgt cgttgcattt ctatgtctaa ttggctacac aggcacgcgg tacgcaaatg
      241 aaattatttt gtggggtgga gagacagata ttgatacaat caatgagaat ctgacacagc
      301 ttgatcatac attgacagat aaagagcaag cgattacaga tcttaccgct cgactagcac
      361 aaaaagaaca gacattgcaa gattcaaagg cagatgtcca agaataccaa acaaaagtaa
      421 atgaattaga aaaccaaaaa aatcaatcgg cagcagaaaa acaaaactta gaaagtcaat
      481 tagtcagcaa ggatgcacaa ctacaaagca aacaaaatga aatcaatgtc aaactagatg
      541 agatcaacca aaaaaatgat gaaatcaatc atctgaatgc agattggaca gcaagactac
      601 aagaagcaca agcaaaactt tcagaattac aaaatactgt aaatggtttg aatcaacaaa
      661 tcgcaacact gacacaagaa aaaacaaatg tagaaactca gttaacagat acacaaaaga
      721 aattagacga agcaactgct gaaaactcaa gcttgaaaca atatattcaa aagttagaaa
      781 aagcaaaaga tgaagtggaa gatacagctg ataaatctca gcaaattgtt gaagaacatt
      841 tgcgtaaata atctttctta acaagtaaac ctaaattagt aaagaagcaa cagtaatcta
      901 tactaagtag ttcagttttg gagtacaaaa tgacaaaatg tacttcaaaa tggactactt
      961 ttttcatttt aacttacata ccaaattaca tacaaaatga tttataaaat gcaattgaaa
     1021 atgataattg aatgaaaatt tgtgattttg tggtattatg ataatataac ttatagaaaa
     1081 aattgcttat tcaactaacg ggcaggataa tga
//
LOCUS       DAZKMB010000253         1111 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000253 DAZKMB010000000
VERSION     DAZKMB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.261"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(266..970)
                     /locus_tag="ACG8V3_002924"
     CDS             complement(266..970)
                     /locus_tag="ACG8V3_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301372.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          321 a          193 c          199 g          398 t
ORIGIN      
        1 ttttttctct atcagaaaat atgtttatta ttagaatttt ttatagttaa ggttaggcat
       61 cttaagaaat caggcaactc caagatatct tcaggtagaa gagaagtcgc ctaacattgt
      121 aggatcatag gtgatttgtt ggacaggtca agctgatatt tgagcctgat gttagaagga
      181 atttcttttt acttgctgta gaaattatta tatgactaaa aaggctggga ttttaaatcc
      241 cagcctttta tgacaaatag aatttttatc gatcagtaat cgctgtcgct gcaaattgtt
      301 cgatcaaagg aatcaaagtc aatttgactt tgtatttccg ttcgagtgct ttttcgataa
      361 cgaactcatt ttcagtagtc atagaaatag ttgctaattt acgaggttca tcagcatttt
      421 tttcattaat aactacttca tgttcatttt gttctgtgta aaatttagga tactcagcaa
      481 cggcttttga aaaatcagca aaacttaaag cggcattcgt atcagttgag cgtgtatttt
      541 cagttgtgtc cgcttttggt ttttccgttt tttcttctgg cacactgact gtttcttctg
      601 taactggtgt cgcaacagct tgcgcagttg gttcatcgtt tttcaatcgt tgatcattcc
      661 agtaagacac gatttcaata agaagattgt cacggaatcg ttcgaaaatc ccgttttctc
      721 ctgaacgaat tctctttaat acagtattga agttgtcatc gattggcacg ttgatccgtg
      781 tatcttcatt tgttttccca gctaatccga tcgttaagat ttgacggttt ttgggatcaa
      841 gcatgacttg taacggcaaa ccataacgtc gcaagctctc ttcttctaga gaacggaata
      901 attgtcctct tacaccatcc aatgaaggac ggaattgatt taataatttt tgtttctgtt
      961 ttttcttcat atgttctctc cttcgctcat accctttcga ttataagaaa aaaacagcca
     1021 aatgaaaagg agtaatgcaa aaaaacttgc gtatacgcaa attacaacag aaaaatttgc
     1081 gtattttccc gttttttgaa agaaaaataa a
//
LOCUS       DAZKMB010000254         1110 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000254 DAZKMB010000000
VERSION     DAZKMB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.262"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..>1110
                     /locus_tag="ACG8V3_002925"
     CDS             142..>1110
                     /locus_tag="ACG8V3_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0301373.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFY"
BASE COUNT          434 a          152 c          190 g          334 t
ORIGIN      
        1 gtgatataaa tattgcaggc ttttttattg atttgaaata atcaagaata ttagaaactt
       61 ctacattaat caagaacttt cttctgtatg aaaatagaga taataattgt acacttaagc
      121 tgtattcagg aggtgaagat aatgggcatc gaatttagcg aacggcaaat gaaaatacta
      181 tactttttga ttgaaaatga gcacacaact tacaaagaat tatcagaaca attatatgta
      241 tctacgaaaa caattagcaa agatatttta attttgaaag aaataatcca aaaaatgcca
      301 gatgtacaaa tgttggttca acctaaaaaa ggcgtttcgc ttattggaaa aaaagaaaca
      361 cttcgaaatt tattatccag tcataaaatc aatatcgcag actctccgga ggaccgtcta
      421 acttatgtgt ttgctaaatt attgaatact aatgattctc taaagattca aaatcttgct
      481 gacgaattat acgtgagcag gtcgacggta gaaagcacaa tgaaagatgt tagaaaaaga
      541 ctaaacaagc atgggatgtc cttagtttca gatcgaaatg gcgtgcgcat caaagctgat
      601 gaaaaaagta aaagaaggtt gatgtcagaa gttgtgaact attattgggg tggctttaca
      661 gcatctaaca gtaaaacaga tctcttagac ttgaatatca atttcattgg tgaagcgaat
      721 aatttcttaa ataaggacat attgaaaaaa gtagccgaat tgcttaatcg atacatgaat
      781 aagacacaat tgaaatttac agattatgaa tttcaaactt tagctatcca ccttgctatt
      841 gcgatagaaa ggattgaaac aagcaatttc ttatcacgta gtaataattc tgaagaagaa
      901 atctcaaaga atacacttat tttaatcaaa atgattgaaa aagcattttc aattattgtg
      961 ccaatagatg aacaagctta tgttgacatt cacattactt cgattgaaaa aaatgctatg
     1021 aatcacgact tagatcaaac gaagctttcc tatttagata tgggtgttga gctacaagag
     1081 ttagtaagaa ttaaattaaa tgaattttat
//
LOCUS       DAZKMB010000255         1093 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000255 DAZKMB010000000
VERSION     DAZKMB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.263"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..942
                     /locus_tag="ACG8V3_002926"
     CDS             127..942
                     /locus_tag="ACG8V3_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301374.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
BASE COUNT          418 a          161 c          181 g          333 t
ORIGIN      
        1 agaattaaat aaaaaataat tactatctag tacgaaatta attacatagg ataatatatt
       61 aaggtgctgg actacaagta aaaaatactg tatgatcatg acagattcaa gaaaggagca
      121 ataagattgg ttaagatagc aggactttct aaaggtggaa aaaccgtttc acaactttat
      181 aaacaaaact acagagactg gagacagttc agaaaaaaca ttaaggattc ttactttatt
      241 ctaccaacag aacttgaaaa atatttaccg aataacaaag ctattaattt atatctgtat
      301 tactgtttta aggcaaagaa tgaaggcggg gattcctggc attcggtcaa taccatagca
      361 gagtctctag gagtgacaac taaaactata aacacctgga ataaaacctt agttgattta
      421 ggtatgattg cacgtataga cgataaccat ctcagtaaat cgacaatttt acttcctctc
      481 tctagttact ataaattagc ccttgatagt tctttaaagg atgttaccga gagcactgtc
      541 catgaaattg atggcaactt ggttgcagtt tatcatttgt ttgaatggag aaaaaatgag
      601 gctgatgaaa attataacgt tccttataac acactctgtt gtgtttttaa aagaaaaaca
      661 caaaaagata ctatctataa atttatcttg gtccaagatg agaaaataag taatttcgaa
      721 ttaagcactc cttctagtaa gatttatgac gatgcatata gatttgaatg tcaagatcta
      781 gaagccctcc tttccgcaaa taatctaaac aatatagaac atgaaaactt tgttgtaaac
      841 actagattta atttagtttc tgaaaaggac tcagatttgt tagatttgct gatagagctt
      901 actaaaaatc tagataagaa agaaaacatt actgtactat aactagcatc ggtccatcat
      961 taagtttgga ctattagaac acttgctatt acagaaaaag gtatgctagc cacgcttact
     1021 atttattggt atcaaacaag tatcttaatt tgaaaggaag atgatttatg gctaatttat
     1081 atatagagaa caa
//
LOCUS       DAZKMB010000256         1084 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000256 DAZKMB010000000
VERSION     DAZKMB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.264"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..564)
                     /locus_tag="ACG8V3_002927"
     CDS             complement(<1..564)
                     /locus_tag="ACG8V3_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFQ0301375.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYT"
     gene            complement(557..>1084)
                     /locus_tag="ACG8V3_002928"
     CDS             complement(557..>1084)
                     /locus_tag="ACG8V3_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0301376.1"
                     /translation="PRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQW
                     WALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLP
                     LELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLL
                     NRIDKEILKVGNIDE"
BASE COUNT          396 a          171 c          131 g          386 t
ORIGIN      
        1 agtatatcca aacatagctc caacatttgg acttgtatag acaccaaata accgatgttc
       61 aataaaacct tgtcttgcaa gtaatcctgg cgttcctgtt gggacatgat aagaaatcaa
      121 aaaaataaat tgatagatag atattagcgc agctaaaaaa attatgataa tgaaaacatt
      181 attaaaatac accatttttt tctttttttc ctcttcactt tgtgaaaagt ctgcaggaaa
      241 aattactatc aaagtaatca gcaaatatcc aaaattataa atagatggaa ttaaaaaatt
      301 ttctctgtta atgattatag ttaagataaa actaatcatt gctccaatca gtagccaaga
      361 aaaacgcatt ttaaaaaaat ttttctttat aaaaacctca tacaaaacta taattccacc
      421 ccaagcaaaa gctattttca tcgggatatt agttacatta aaagtactag gcataaaata
      481 aagcagacaa atgattaaat ttattactcg aaacgcatct cgacttaata aactcttctc
      541 taaaacaggt actattctat tcatctatat ttccaacttt caaaatttct ttatcaattc
      601 tatttaatag tttatcagag taatccgatt taacttttct tacaaataat ttttcacttt
      661 cttttatttg atctatatcc ctttcatcaa accatttgtt catggaagga tcaataatat
      721 gaatatttgc taatttcgaa gtttgtaaag tagtttcttc aatatttttc aattcaagtg
      781 gtaaatctgg tcctccaata acatttttat ttttgaacga ggaattaaaa aaaagtgtat
      841 gaaaaaattt ttcatctggg gcaaacatac ctattttaaa attcaggtag tcttgttgat
      901 tttgttcata catttgtatt aattctgtta ggcattcttt cgacaaagcc caccattgag
      961 aaccatgata caaatcaaat ttatggtgtc taatttttgg gggaagttta atcatcctca
     1021 gacaacgatt aattatagct ctaaaaattt taaatgcagt tgtgttattc ctattaattc
     1081 tagg
//
LOCUS       DAZKMB010000257         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000257 DAZKMB010000000
VERSION     DAZKMB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.265"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG8V3_002929"
     rRNA            <1..>1073
                     /locus_tag="ACG8V3_002929"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZKMB010000258         1063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000258 DAZKMB010000000
VERSION     DAZKMB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.266"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..894
                     /locus_tag="ACG8V3_002930"
     CDS             <1..894
                     /locus_tag="ACG8V3_002930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0301377.1"
                     /translation="AKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
BASE COUNT          393 a          115 c          225 g          330 t
ORIGIN      
        1 gctaaaatta taggtatctc tttattgagt aaacctaata ttattcatat gcacatgtcg
       61 tacagaggga gttttagtag gaaatatgtg ctgaataagt ggtttagatt attaaatatt
      121 cctgtaatca tccatcttca tggatcggaa tttaaaaaat ggtatgatag tgtttctgta
      181 aaaaaacaaa atcagattcg cttgctgtta agagagtctg attccacgat tgtacttgga
      241 gaaaaatgga agaagataat taaagaaatt gagccaaatt gtaaaactgt tgttgtagaa
      301 aattctattg tagttccaga atataccata aagtggaatt caaaaatgtg ccgatttctg
      361 tttatgggag ttttgataaa gcgaaaaggt gtacacgatt tggtagaagc agttaaaaac
      421 attgcagaac aatatccaga aaaaaagttt atttttgaga ttgctggagc aggtgatgaa
      481 gaaatatcgc tcaaagaatt gatagaagaa tataatttgc aatcgtattt tgaatttaca
      541 ggttgggttg tgggagaaaa aaaggaggag tgctatagaa gatcaaatgt gatgatttta
      601 ccgtcctata acgagggatt gcctatatct attctagagg cgatttctta tggaatgcct
      661 gtaattgcta cagatgtagg agatatttct tcagcagtac gtgatggaga aaatggatat
      721 ttgtttcaac ctggtgacgt atcagcgttg acatctgcaa tactaaaaat caatgataaa
      781 gataaatacg taaaattttc acgttgttcg aaggatttgg caataacgaa atttaacgat
      841 ggaaatttct ttgatagaat tttaaatctg tatcaaaaag ggagcaagga gtgatgacaa
      901 ggaaaataaa aaatgtttcg attatgtaat gaacttatta agtgaataaa atagagagaa
      961 aaaatattga aatagacgat gtaaattttt atccgtgtaa gaattcaaat gaatagtagt
     1021 gttttagtgc ttgtaataga gcaagagtac aagcacggta cat
//
LOCUS       DAZKMB010000259         1062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000259 DAZKMB010000000
VERSION     DAZKMB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.267"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>1062)
                     /locus_tag="ACG8V3_002931"
     CDS             complement(37..>1062)
                     /locus_tag="ACG8V3_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0301378.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAZKMB010000260         1033 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000260 DAZKMB010000000
VERSION     DAZKMB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.268"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1033
                     /locus_tag="ACG8V3_002932"
     CDS             <1..>1033
                     /locus_tag="ACG8V3_002932"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0301379.1"
                     /translation="EIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQEYDKVILATGS
                     KQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKR
                     GKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTT
                     SGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTF
                     FTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLK
                     TARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLS
                     MFSLAIEEQI"
BASE COUNT          400 a          122 c          181 g          330 t
ORIGIN      
        1 tgagataaaa aagttatttc tagagacaga agttgacaaa attgatattc aaaataaaaa
       61 gattagttgt gtaaaaaaag atggtacaag attctctcaa gaatatgaca aagttatatt
      121 agctacaggt tcaaagcaaa ttatgctaga tataccgggg aataatttag ctaatatttt
      181 ttcgataaag aattttccaa atgcaaaaga gatttttaaa gctttggatg atagtactat
      241 taaaaaagta ggaattattg gagcaggata tattggagtt gaaattgctg aagccataag
      301 aaaaagaggg aaagaagtat atcttttcga tgttgcagat agagtgttgt cgacttatta
      361 cgaccgtagt ttttcagata aagttgaaga aatactatca aaaaatggaa tacatctttg
      421 tctatcagaa agtcctaaaa aatatttagg aagaaaaaag attgaaaaaa tagtcactac
      481 aagtggtacc caatactcta tggatatggt cgtacaagct ataggatttc tatctaatag
      541 tccaattggg gaaaaggaac tcttgagaga tgttagtgga gcatatctaa ctaatgaata
      601 ccagcaaaca aatataaaag atatatatgc tattggggat tgtgctacaa ctttttttac
      661 gtccattaat agaagtagtg ttgatttttc tatttctaat gctctaagaa cagcttatat
      721 tgcaactcaa catattaaca atcaagattt tacaccttct ggttcccaat ttacaaatgg
      781 cttttctata tttaattaca atttttacgc agcaggtttg aatttaaaaa cagcacgatt
      841 attaagtgag tctatagatt atatagaaat agaagatttc attagacctg cttttttgag
      901 aaaaaatagt cgtgtaaaat taagaattag ctttgaaaaa aatacaaaga gaatagtagg
      961 tgtacaactt tgtagtcaag aagatttatc tggactatta tcaatgtttt ctcttgctat
     1021 agaagaacaa att
//
LOCUS       DAZKMB010000261         1002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000261 DAZKMB010000000
VERSION     DAZKMB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.269"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1002
                     /locus_tag="ACG8V3_002933"
                     /pseudo
     CDS             369..1002
                     /locus_tag="ACG8V3_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DAZKMB010000262          988 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000262 DAZKMB010000000
VERSION     DAZKMB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.270"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..988
                     /locus_tag="ACG8V3_002934"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACG8V3_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          351 a          175 c          197 g          265 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaa
//
LOCUS       DAZKMB010000263          957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000263 DAZKMB010000000
VERSION     DAZKMB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.271"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG8V3_002935"
     CDS             <1..878
                     /locus_tag="ACG8V3_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0301380.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          300 a          212 c          191 g          254 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcggctctat actttttgat gttggtgaac ttcaccaaca tcttttt
//
LOCUS       DAZKMB010000264          924 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000264 DAZKMB010000000
VERSION     DAZKMB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.273"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>924
                     /locus_tag="ACG8V3_002936"
     CDS             <1..>924
                     /locus_tag="ACG8V3_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0301381.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQ"
BASE COUNT          314 a          159 c          195 g          256 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaa
//
LOCUS       DAZKMB010000265          910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000265 DAZKMB010000000
VERSION     DAZKMB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.274"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG8V3_002937"
     CDS             <1..355
                     /locus_tag="ACG8V3_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301382.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACG8V3_002938"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACG8V3_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DAZKMB010000266          898 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000266 DAZKMB010000000
VERSION     DAZKMB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.275"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..879
                     /locus_tag="ACG8V3_002939"
     CDS             256..879
                     /locus_tag="ACG8V3_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0301383.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          332 a          129 c          167 g          270 t
ORIGIN      
        1 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
       61 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
      121 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
      181 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
      241 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
      301 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
      361 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
      421 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
      481 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
      541 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
      601 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
      661 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
      721 aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga tgaaattgtt
      781 gatggtctga agaaaaaaga aactatttct aaaatagcaa agaacgcagg agttacaaga
      841 aaaaccgttt atcgagtaaa aagagattta ggaatgtaat atgttccagt tagaaaga
//
LOCUS       DAZKMB010000267          894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000267 DAZKMB010000000
VERSION     DAZKMB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.276"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..838
                     /locus_tag="ACG8V3_002940"
     CDS             98..838
                     /locus_tag="ACG8V3_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301384.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          328 a          164 c          184 g          218 t
ORIGIN      
        1 atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat ctgtgctatt
       61 attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca gacgaaaaca
      121 tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg acaagaagcg
      181 agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc cgtttgaaca
      241 gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca agcgtcgtgc
      301 aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata aaggaaaatt
      361 accggattat accggccatt accaatcaga aatcacatcg acaggacgaa aaaaactttt
      421 tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca agcatactac
      481 acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc ctaaatatgt
      541 acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc atttaatcga
      601 atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga cgccgtatca
      661 agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa cgttagagga
      721 aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag aacgctcatt
      781 acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt ttaaataagt
      841 gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt ttcc
//
LOCUS       DAZKMB010000268          879 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000268 DAZKMB010000000
VERSION     DAZKMB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.278"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..655
                     /locus_tag="ACG8V3_002941"
     CDS             <1..655
                     /locus_tag="ACG8V3_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301385.1"
                     /translation="EGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVIAGYGRMDIFPK
                     LYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTVVQGVDPQITNY
                     LSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEETAIIAETLLNLT
                     SFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRLRKERANDYNN"
     gene            639..809
                     /locus_tag="ACG8V3_002942"
     CDS             639..809
                     /locus_tag="ACG8V3_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301386.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT          325 a          119 c          146 g          289 t
ORIGIN      
        1 ggaaggtgtt tcagaagcta tatcagagga aattcaaagt tacgtttatg aaattataat
       61 tagagacgat gtttattctt cccctacagg aatagttata gcaggatatg gaagaatgga
      121 tattttccct aaactatatt cttataatat gtttggattt gttatgaata ttctgaaata
      181 ctcagaatat gaaagtgctc aaataggaaa tgacaatggt tcattaagat ctactatatt
      241 accatttgct cagtctgatg tggtaaatac tgtagttcaa ggggtagatc cacaaattac
      301 taattatctg tcgagtcaag tcgatagttt tgacgataac ggaaaaaata cctatattaa
      361 tattataaag aatatttcag agtttcaaca aaatcaattc atatttccgt tactaaacat
      421 gattgcttta ctcccagttg aagaaacagc aattatcgca gagactttat taaatttaac
      481 cagttttaaa cgaaaatata ctacttctgt agaaacagta ggtggcccaa ttgatgtttt
      541 agctattaca cctaatgatg gccctatttg gattaaaaga aaacattatt ttgatattga
      601 taataatatt ggatacagat tgcgaaagga gagagcgaat gattacaata attaaacctc
      661 aggaaaaatc ccctgttttg aaatttaatg agagcaatct agcccataat aaaatgagta
      721 taactgatac agtatttgat gtaatggttg agcaacaaaa aaaaattaat aataacaata
      781 gacaaagaaa agaaattaac caatattaat gttttttctg ttgcgttttt tgcagtcata
      841 atttaaaacc tcgctcaatc ttgggcgagg tcttttttt
//
LOCUS       DAZKMB010000269          874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000269 DAZKMB010000000
VERSION     DAZKMB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.279"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..133)
                     /locus_tag="ACG8V3_002943"
     tRNA            complement(61..133)
                     /locus_tag="ACG8V3_002943"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(98..100),aa:His,seq:gtg)
     gene            complement(136..209)
                     /locus_tag="ACG8V3_002944"
     tRNA            complement(136..209)
                     /locus_tag="ACG8V3_002944"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(175..177),aa:Trp,seq:cca)
     gene            complement(216..296)
                     /locus_tag="ACG8V3_002945"
     tRNA            complement(216..296)
                     /locus_tag="ACG8V3_002945"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Tyr,seq:gta)
     gene            complement(298..373)
                     /locus_tag="ACG8V3_002946"
     tRNA            complement(298..373)
                     /locus_tag="ACG8V3_002946"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Phe,seq:gaa)
     gene            complement(409..481)
                     /locus_tag="ACG8V3_002947"
     tRNA            complement(409..481)
                     /locus_tag="ACG8V3_002947"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(446..448),aa:Asp,seq:gtc)
     gene            complement(491..563)
                     /locus_tag="ACG8V3_002948"
     tRNA            complement(491..563)
                     /locus_tag="ACG8V3_002948"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(528..530),aa:Val,seq:tac)
     gene            complement(579..650)
                     /locus_tag="ACG8V3_002949"
     tRNA            complement(579..650)
                     /locus_tag="ACG8V3_002949"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Glu,seq:ttc)
     gene            complement(665..754)
                     /locus_tag="ACG8V3_002950"
     tRNA            complement(665..754)
                     /locus_tag="ACG8V3_002950"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(718..720),aa:Ser,seq:gga)
     gene            complement(794..867)
                     /locus_tag="ACG8V3_002951"
     tRNA            complement(794..867)
                     /locus_tag="ACG8V3_002951"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(831..833),aa:Asn,seq:gtt)
BASE COUNT          244 a          240 c          200 g          190 t
ORIGIN      
        1 acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa gaataaaaag
       61 ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc gttaaccact
      121 tcgccacatt cgccatggca ggggcagcag gaattgaacc cacactaacg gttttggaga
      181 ccgctgttct acctttaaac tatgccccta taaaatggag gagagtggat tcgaaccact
      241 gaaccctaag gaacggattt acagtccgtc gcgtttagcc acttcgctac tcctccatgg
      301 tggcgcggga cagaatcgaa ctgccgacac acggagcttc aatccgttgc tctaccaact
      361 gagctaccgc gccatatttt aaaattatta attgaataac aagaacgacg gttccgacgg
      421 gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt taacccctac accacggaac
      481 cagttcatta tggaggttaa cgggatcgaa ccgctgaccc tctgcttgta aggcagatgc
      541 tctcccagct gagctaaacc tccaacattc aattaaaatg acccgtacgg gattcgaacc
      601 cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga ccaacgggcc aggtaaattt
      661 gctacggaga gtaagggatt cgaacccttg agacagtttg gcaccatcta catgatttcc
      721 aatcatgctc cttcggcctc tcggacaact ctccagagtg aaagaagctc aatactcttt
      781 ctcaaaatga gaactccggc agtaggactc gaacctacga catcatgatt aacagtcatg
      841 cgctactacc aactgagcta tgccggaata acat
//
LOCUS       DAZKMB010000270          873 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000270 DAZKMB010000000
VERSION     DAZKMB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.280"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>873
                     /locus_tag="ACG8V3_002952"
     CDS             <1..>873
                     /locus_tag="ACG8V3_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0301387.1"
                     /translation="KSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQ"
BASE COUNT          263 a          174 c          176 g          260 t
ORIGIN      
        1 gtaagtcttg ccaaagaaca ttcgtagcgg atacctctgt tgctgaaaaa cactgtttca
       61 ttagtcaaaa agttcgttgg tctgtcattg ctcgcttaaa agagaatact tccatgacgg
      121 aaatcgctcg tcagaaaaat atttccactt cttcggtcta tcgtgtcatg aagcgattct
      181 accgcccatt gaatcctttc aaacagactt tgccaaaggt cttatgcttt gatgaattca
      241 aatcggttcg aagtgttgcc agtgcaatga gttttatcat gatggacgga caaagccatg
      301 cattattgga tattgtcgaa aatcgacgct tgccttattt agaacgctat ttttctcggt
      361 tttcattagc gactcgtgaa gccgttcaat tgattgttat tgatatgtat gcgccttatg
      421 tttcgttggt gaaaaagctt ttcccgaatg ctcagttgat catcgatcgt ttccacatcg
      481 tccaacatat tggcagaact ttcttgaatc atcgagtgaa agaaaccacg gtacgactta
      541 aaggaaattc ccactctcaa cgagggttag ggaaaaaatt gaagcgctat tggaaactcc
      601 tacaaaaaga agaagcaaag ttaaactatg aaaaacgggt ttggcgtgcc agttttaaag
      661 attatttgac tgaaagtgag attgccgatc gactgttagc tgcttgtccc aacttacgcc
      721 aaggctatca gttataccaa gacattcttt atgcggtaaa gaaaagagat cagtcactat
      781 ttgaggattg tttaactaga gacattagcg gtctgccaga aacctataca accactttac
      841 gaacgtttaa aaagtttctg ccacagatcc aaa
//
LOCUS       DAZKMB010000271          863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000271 DAZKMB010000000
VERSION     DAZKMB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.281"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            345..758
                     /locus_tag="ACG8V3_002953"
     CDS             345..758
                     /locus_tag="ACG8V3_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0301388.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT          311 a          134 c          184 g          234 t
ORIGIN      
        1 caatttgtac aataaaaaat actataggta aaaaagataa catgaggagc ttatttgttc
       61 tagagtgttt gacaaagtaa caaaatgcga cgataccaat acaaacggga atccaaaata
      121 acagcatgct ccacatagtc actcaaccat ctcctatacg ctttattatc aatagataat
      181 atcatttttc gtaataaaga taaaaaaata agtaaggctt ctcattaaaa tataaaaaag
      241 acagccgacc actgactgcc tatataagag tattgaaata aaaagctgct gatataataa
      301 attccacaag tttattatat cacataaagg agcggtttga cttgatgcaa ttgttacgag
      361 aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac tttcggcgtt
      421 tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt acggatatgc
      481 cgaaggcgcc gaagcacggc aataaggcag aagacgcaat cattcagatg atggatatag
      541 aagcggagag agacgcgatt ctagcagcct tgatggctct tagtctgatt agtcgtcaga
      601 tactctacta tagcttttgt gtgccagata gcttctcaaa ctacagaatt agccgtgaag
      661 tgggttattc agaaagaagt atacaacgga tgaagtcgga agctctaata gagtttgcag
      721 aagcatataa acacggaaga ataattgctt ataaataatt tggcggcttt ttggcggaat
      781 gatggcggtt tttagctatt taccagtgat attatggtag tgtcgaaaga ttagtgatag
      841 gtctaagaca aaataataat aaa
//
LOCUS       DAZKMB010000272          862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000272 DAZKMB010000000
VERSION     DAZKMB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.282"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..220
                     /gene="pssD"
                     /locus_tag="ACG8V3_002954"
     CDS             <1..220
                     /gene="pssD"
                     /locus_tag="ACG8V3_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0301389.1"
                     /translation="DLVICTGVLAMIPICLITKMMGKKLIYIESFAKVTSPTQTGKLM
                     YHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            237..716
                     /gene="pssE"
                     /locus_tag="ACG8V3_002955"
     CDS             237..716
                     /gene="pssE"
                     /locus_tag="ACG8V3_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0301390.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
BASE COUNT          325 a           99 c          169 g          269 t
ORIGIN      
        1 ggatctcgtg atttgtacag gtgttttagc tatgatccct atctgcttaa taacaaaaat
       61 gatgggaaaa aaactgattt atattgaatc gtttgcaaaa gtaaccagcc ctactcagac
      121 aggaaaattg atgtatcatt ttgcagatca attttatgtt cagtgggaaa ctatgctaca
      181 attttatcct aatgctattt atttgggtgg gatatattaa ataataggag aaaaaaatga
      241 tatttataac attaggttca caaaaatttc aatttgatag attgttaaaa gctattgatg
      301 atttaattga aaaaggagta ataatcgaaa gtacatttgc acagagtggt tatagtgatt
      361 ataaaccact aaattattcc tataaagcat ttctaaatag ggatgagttt actgaaacta
      421 tgcaaaaagc agaaatggtg attactcatg gaggtacagg tgctataatt ggggccgtaa
      481 aaaaaggtaa aagggtcatt gctgttcctc gattggctaa gtatggtgag catgtcgatg
      541 atcaccaact gcagttaatt agacaatttg atgagatgaa tattatctat ccttgttatg
      601 aattagaaaa tttagaagat gccgttaaag aaacaagagt tcggatgttt aacaagtatg
      661 tatctaacac ggaaacgatt attaattcga tagaggtatt aatagaaaaa atataaatat
      721 aaggagtaaa gcgttatgca aagagtactt atgattggta atgacagctc ggtaaaaggt
      781 ggaattacaa gtataattac acaaatgctc aattttaatt gggaaaaaaa gaatatcgag
      841 atgactttta taccgagtta ta
//
LOCUS       DAZKMB010000273          858 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000273 DAZKMB010000000
VERSION     DAZKMB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.283"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..307
                     /locus_tag="ACG8V3_002956"
     CDS             8..307
                     /locus_tag="ACG8V3_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301391.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(404..838)
                     /locus_tag="ACG8V3_002957"
     CDS             complement(404..838)
                     /locus_tag="ACG8V3_002957"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0301392.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT          282 a          150 c          127 g          299 t
ORIGIN      
        1 ataaaacatg gattatactg ttgaatttga cagtgaacta cttaagcaat ttccacaaga
       61 agaacatttc atgtataaag aagcaatttc ccacaaagtg acagagtatt tggagcataa
      121 tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa ggaatatacg agatgaaagt
      181 caaaattaat aaaaaaaatt atcgcatagc atttgcttta aaaaaaaatc atgtgttcat
      241 tttttatatt agttcgaatt tgcaaaaaat tgtatttgat aaagaggtga gtaaaaaaat
      301 aaaatagctg agcattggag gatagattga gtgatactaa gtctatcctc ttgtttttct
      361 aaagaactaa gctagaattt tttcttttgt acgttattat atgttatttg tcattaccaa
      421 aaagcacctt ttttcttcta gtatctatga cataagaaat accacccatt tcctttactt
      481 gaacaaatcc tactgattca taaaattttt ttgttttctc agtatcatct gtcaataatt
      541 cgatttggac tggtccataa ctagtgatct tctccaaagc ttcattcaaa agcactttgc
      601 ctattccttg gcgctgatac ctcggatgaa tgaggatatc ttgaataaat gcgatcgtcg
      661 caaaatcagt gatccaacga acgattccga taatcttccc ttccccatta cgctttatca
      721 gagattcaga ttgttcaaat gctctagcta accttgcagt gtcttttgtg taattcgtcc
      781 aaccaacgct cttatataat tcagaaatct catcgatcat aagggaatca atattcattt
      841 ttttcttcct ttcttata
//
LOCUS       DAZKMB010000274          849 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000274 DAZKMB010000000
VERSION     DAZKMB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.284"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..410
                     /locus_tag="ACG8V3_002958"
     CDS             87..410
                     /locus_tag="ACG8V3_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301393.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            574..828
                     /locus_tag="ACG8V3_002959"
     CDS             574..828
                     /locus_tag="ACG8V3_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301394.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
BASE COUNT          218 a          125 c          139 g          367 t
ORIGIN      
        1 ttcttttact tgtgtcataa ctattttgtt actttaattt ttatttgctt attttagaaa
       61 aaatgttatt cttttattta ttctcaatgt taggagtgat tattttgcca tttattgcga
      121 ttttattcga tttagttgcg gcagcagctt attttctcca attaaattat caaacctcag
      181 gaattctgtt tattggaatg atttttcaag gtgtgattac ccttttgctt cttgtcatga
      241 tgctcagcta taaaggaaaa agatatgccc gtattcaaac aaaagtattt gttaaatatg
      301 tttccattcg ctatgggatc atcattcttt cctttttagt gaatgcgatc gtcctctttt
      361 tatatgtatt gaattactta gatatcaatc cattgatttt ttctcgatag ataagacaca
      421 ttcagcggaa cagttcctgc ttatcagcag aaaactgttc tgctttttca tttttcattg
      481 atctattctt cttttatttt cctagcattt catttgtgat gtatgcaata acatgttatc
      541 ttttattata tgaatcgttg aggagtccgt tacatgatta cgttggtgag agttttgttt
      601 tgggtgcctg cggtcgcact ggtcgcaagt attgtttatc ttatgaattg gaacaaagaa
      661 cggttttatt tagctatttt gactcttccg gctatctact ttatgtggaa agtttttaat
      721 tacaactact ttgaacccga tagtgttttt atagaagaat tatcaggact ggttttgtct
      781 ttattgatcg tcattcttta tttgatacgt ttaaataaaa aacattgagt actattgatt
      841 gcatgtgta
//
LOCUS       DAZKMB010000275          840 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000275 DAZKMB010000000
VERSION     DAZKMB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.285"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..380
                     /locus_tag="ACG8V3_002960"
     CDS             <1..380
                     /locus_tag="ACG8V3_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301395.1"
                     /translation="EDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSEIE
                     DTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVV
                     KFNGTEIGRQIFDIKVNKPVEKN"
     gene            394..795
                     /locus_tag="ACG8V3_002961"
     CDS             394..795
                     /locus_tag="ACG8V3_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301396.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          306 a          112 c          152 g          270 t
ORIGIN      
        1 cagaagatgt agaaagtcag caatttggca ctaatgttat taataaagta attaatccag
       61 ctctttctat taatgcgcct ttaattccta ctgctttatc attttctgta acagctgtgg
      121 tgtctgagat agaggataca gaaaagataa aaatagtaga aattgaagtt ttaaacaaga
      181 atgaaaaaca aattttttct acgggtgaag tatcggtaag cttgcctcct caagttaacg
      241 atataaactt taatataaac gccagaaatg tcttggttga agaagcagga gagcattatg
      301 ctgttgttaa atttaatgga actgagattg gaaggcaaat ctttgatatc aaggtcaaca
      361 aaccagtgga aaaaaattaa ggagacgata cggatgcttg atatagtacc atcaaataca
      421 catcggagtg gtagcttaat aaattttgct gcgatttttg cttgcgcatc ggtaattcta
      481 gcatctccag ttgttgtgga tgcaagttct actccgaaaa ctaaaaatga taatcaactt
      541 gtaattacta caaaaaatga gattaacaca atttcaagta atgatggcaa aatttttgat
      601 gtgataaata cggttttaaa aagaaaagct gaaatcaata taaaatatga tgaagattta
      661 aacttgtatt tctttgttat caaaacaaca tctgaattgt tttcctcaga ttacgatgtt
      721 ttggatactc tcgataatgt attatctgac tataaatata tggggaaatc tgtagtagca
      781 actttggggg aataaaatgt ttgactttaa tgaatattta tctatttcca aacaaattga
//
LOCUS       DAZKMB010000276          831 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000276 DAZKMB010000000
VERSION     DAZKMB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.286"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>831
                     /locus_tag="ACG8V3_002962"
     CDS             <1..>831
                     /locus_tag="ACG8V3_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0301397.1"
                     /translation="SFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIV
                     MNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNK
                     RKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKA
                     KAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADT
                     ISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSR
                     "
BASE COUNT          319 a          101 c          161 g          250 t
ORIGIN      
        1 aatctttttc tatagttcac aatcaggttt attttggcgg gggaattaat ttatggttag
       61 ccaaaaagaa tggaatcaga cagagaattg ctcatagcca tgcaactgaa gatggaaaat
      121 ctcaaaatat tgtaatgaat gtattgcgta aatttttaac caaattgttg ttacaaaatg
      181 caacagacta ccttgccgtt agtcaagaag ctggtgaaag tctgtttcaa aaccacccct
      241 ttgagatagt tcataatggt attgatttag agctttatag taaaaattca gaagctaagg
      301 ttaataagag aaaagaattg gatatctcaa tgagtacttt tgttgtggga aacattggac
      361 gattagagaa acaaaaaaat caatcttatt tgttagagat ttttttagaa atactaaaaa
      421 tgaattctga ttcttacttg attttagtag gtgaagggtc acttagaaat aaactgaaag
      481 ccaaagcagt agaattggat atagagaaaa gagtaaaatt tttaggagaa agaaacgata
      541 ttcccgagtt attatctgtg atggatgttt ttgtaatgcc ttctctttat gaaggtctgc
      601 caatggttgg attagaagca caagcagcaa aattaaaatt agtcttagca gatactattt
      661 ccaaagatac aaagttaaca aataatgtgg attttgtatc attagatgaa acaccacaag
      721 aatgggcaaa aaaagtaata aaagagccgt atgctaataa aataacacaa gaactaaaga
      781 gttatgatgt ttcgtacact actgaacaaa tggagagtat atatagtaga g
//
LOCUS       DAZKMB010000277          825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000277 DAZKMB010000000
VERSION     DAZKMB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.287"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>825
                     /locus_tag="ACG8V3_002963"
     CDS             <1..>825
                     /locus_tag="ACG8V3_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301398.1"
                     /translation="YLFPAFKNMEIFGFYDTAFHINRALSLESIFSSPINFETFRSYG
                     MQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSS
                     FFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTL
                     LIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIE
                     QTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNFKK"
BASE COUNT          261 a          115 c          118 g          331 t
ORIGIN      
        1 tatttatttc cagcatttaa aaacatggaa atatttggct tttatgatac ggcatttcac
       61 attaatcgtg cgttaagttt agaatctatt tttagctccc ctattaattt tgaaacgttt
      121 agatcatatg gcatgcaagt aaataatttt tacccatggt taactctata tcctttattt
      181 ttattgatca agtttactaa tttagcaata ggctataacc tttttcttta tattgttacg
      241 ctcattactc tatttatatg tcattatgta atgtatgaaa ttaccaaaaa acatgttact
      301 agctcctttt tcgctattat atatacaaca tcttctttta ggagtgtaga aatatttctt
      361 cgaggggcaa tgggggaatt actcgcaatg tcaatacttc ctcttatttt actcgggttt
      421 ataaagcttt acgactcaaa aaaagaatct tgggttatgt tggcgattag catgacttta
      481 ttaatttaca ctcatgttct ttctgtagct atgactatgg taatgattat aattgcttta
      541 attattcaat tttatcggaa aaagaaaata gaaatcttct taattattaa attaataaaa
      601 gctgctatag ttactttact tctttcacta gcgtttatag gtcctatgat tgagcagaca
      661 ttgtatcaag atctgaatag accttatgtg gatatattac aaaaaagagc aatttatctg
      721 ggtaaagagt ttttgattgg aagtatagat tcagaattac tatcatttgg tattggctta
      781 actttgctta tttgtttaat tatactaaca tttaacttta aaaaa
//
LOCUS       DAZKMB010000278          815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000278 DAZKMB010000000
VERSION     DAZKMB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.288"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(494..718)
                     /locus_tag="ACG8V3_002964"
     CDS             complement(494..718)
                     /locus_tag="ACG8V3_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301399.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          293 a          111 c          146 g          265 t
ORIGIN      
        1 ttctttttta atttgttctt gcaatctttt ttgctacgta atttttgtaa aaaagtgggt
       61 tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa atgagaaaaa tatttattcg
      121 tttattgtgg gtactatctt agaggaataa ataatagtgg agaaaactat ggtttggcgc
      181 agattctatc aggtttgatg gaacgaagac aacctcccga catcaagtca aaagctactt
      241 gagattttga tctgaaaata aaaaaagaaa acaaggtagg aacatcaatg tttctacctt
      301 gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg taatcaataa aaaggatggt
      361 gacaacagta cctctttact ctctgaaagc atcataactc gtttttcctc attatgccaa
      421 taaattgttg ttataccaca tataaggatt tgtaatagct aaaaacagac aggaaagttg
      481 aaatctgtga atcttatttt gtatggaagg atttatagaa cgcaaaaatc aagataaaga
      541 tgaataaaaa tcctgtttcg gctatatatt gcgtatctac gccaataaaa cgagtttgtt
      601 ctgtcatagc catataaagc aaaggcaaaa atacaactac agcgagataa gatccgatga
      661 tttctcgaaa agttttccag gaagtatgtt tcctgttttt atatttatcc gatttcattg
      721 aatagtcata gcctccttat tctttcatat gttgaaattc cagtataaaa actggaagga
      781 actgtacgtt ttttagaata aaaattataa gggat
//
LOCUS       DAZKMB010000279          814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000279 DAZKMB010000000
VERSION     DAZKMB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.289"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..528)
                     /locus_tag="ACG8V3_002965"
     CDS             complement(16..528)
                     /locus_tag="ACG8V3_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301400.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            complement(535..>814)
                     /locus_tag="ACG8V3_002966"
     CDS             complement(535..>814)
                     /locus_tag="ACG8V3_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0301401.1"
                     /translation="INSIDNYYIQFKGFDFNSSTEFKLALYAQFFIEKLNRFQEIVED
                     SDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGNLSYEMISKVLSL"
BASE COUNT          283 a          115 c           76 g          340 t
ORIGIN      
        1 ttaaaactaa catccttaat atcttaattt atccatattt tgagaaataa tacttttcca
       61 ttcagaaact aatttagtaa ggaatcttgc aatgttttgt ctcattaatt cattatttct
      121 attatctata agatctgtta gatatttttc aaattctaaa aagctatccg aattttcttc
      181 gacgattaaa tacttaagag aatttatatc ggaaaagata gattcaagtt tactaatctc
      241 attttctaaa tctatatttt cacgtgtgat tatctcatca ctttttctta aaaagctaaa
      301 aattgcatca gttgctttac acttttccat taaagaagta tattcttcaa tttttcttct
      361 ttctttatgg tcatttttta aatcagtttc aatgtctttt ttcatttgtt ctatactgag
      421 tttgttcttt ttgttctttt gagtaaatct aaaattcaaa cacgaaaaaa gtatgttagc
      481 aataacacct ataatagcaa ataaatcatt tatactattt aatttcattt atcatcacaa
      541 acttagaact ttacttatca tctcataaga caagtttcca aattgataat tattggtaga
      601 ttttaaatca ttataatttt cgagaaattt tatataagta cctctcatcg gtttatggct
      661 attaaaagtg ttaaaatcac tatcttcaac tatttcttga aacctattta atttttcaat
      721 aaaaaattgt gcataaagtg ctaacttaaa ttcggtagag ctattaaagt caaaaccttt
      781 aaattgtata taataattat caattgaatt tata
//
LOCUS       DAZKMB010000280          813 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000280 DAZKMB010000000
VERSION     DAZKMB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.290"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..>813
                     /gene="mltG"
                     /locus_tag="ACG8V3_002967"
     CDS             96..>813
                     /gene="mltG"
                     /locus_tag="ACG8V3_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0301402.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIE"
BASE COUNT          288 a          121 c          175 g          229 t
ORIGIN      
        1 tttttgatag aatatttaaa taaaatcatt cacgccaatt caaatatcat gtataatgaa
       61 taaagttacg aacttgatag taaggaggag ttgttttgtc aggaaacaaa caacacccaa
      121 gtaaagataa aaaagaagcc agtaaggcag ctacagcaga tataatcgac aagcggagaa
      181 atctgcgtaa aaaagaagat aagattgtga ggaagatcat tttagtgatt gctcttacat
      241 tattgattat tggcggattt cttggattta ccgtgtatcg ttatgtagat agcgggttga
      301 agcctttaga taaatcagat gaccaattag tccaagtcga gatcccgagt ggctcttcta
      361 acaagcagat tggtgagatt ttggagaagg acaatattat taaaagcggg attgttttca
      421 attattatac aaaatttaaa aatttaactg gctttcaagc tggctattat cagttagcgc
      481 ctaatatgac tcttgacgag attggcaaac agttgcaaga aggcggtact tctgagccaa
      541 caaaagttgc agatgggaaa attgccattc cagaagggta cgatatcgat cagattgctg
      601 aacgggtagc gaaggtaact ggtaaagaca agaaagaatt tttagattta gtgaatgatg
      661 agactttctt taacgagttg caccaaaaat tcccagaact gttggaaagt gcatctaaag
      721 ctgagaacac aaaataccgt ttggaaggtt acttattccc agccacatat gattattaca
      781 cgagtacttc gcttaaggat ctagtgattg aaa
//
LOCUS       DAZKMB010000281          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000281 DAZKMB010000000
VERSION     DAZKMB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.291"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG8V3_002968"
     CDS             <1..672
                     /locus_tag="ACG8V3_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0301403.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZKMB010000282          802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000282 DAZKMB010000000
VERSION     DAZKMB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.292"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..>802)
                     /locus_tag="ACG8V3_002969"
     CDS             complement(151..>802)
                     /locus_tag="ACG8V3_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0301404.1"
                     /translation="TIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDP
                     FLIQNMQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKD
                     FAKLSVTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVV
                     QIPWTTPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT          275 a          138 c          122 g          267 t
ORIGIN      
        1 ttgtcttgtg aaaaataatg attatcctta ccaaaaaatg tttgtttttc tacatgttcg
       61 tacttatcaa aaaatatgat ccttattgaa attactggct atttatattc caacgacttc
      121 ttgtgttaat ttttgttatc tcatagtaaa ttatgtttca ttttcactta atttagttgt
      181 cacgtgttcg tcaattcgta aaaacggtaa atatataaac acacttgcgg ctataattaa
      241 tatttgtaaa acagctgctc gccagtctcc ggctgttgct aaaaatcccg atataactgg
      301 tggcgtagtc caaggaattt gcactactac atgattaatt aatcccacag cagttgcaaa
      361 atatgctact gtcaaagaaa aaatcggtgc aagcacaaag ggaataatca gactgatatt
      421 aaatacaata ggcaatccaa aaataatagg ttcgcttata ttgaatatag cgggagttac
      481 cgataattta gcaaagtcct tataatcttt tctacgactg aagataaata ttgcaatcaa
      541 taaagcaatc gtatttcccg atccacccat taccgcaaag gtatctttaa aggccatatt
      601 gataatatgt ggaatttgct ctttgtttgc ataagctgcc atattttctt gcatattctg
      661 aatcaaaaac ggatctaaca atgaacctga tattactgct tgatggatac caaatccaaa
      721 aaatagatta gcaatagatg taattaacaa aaatcctggt aaaccagttg taattttact
      781 taatggctct gtaactatcg tg
//
LOCUS       DAZKMB010000283          792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000283 DAZKMB010000000
VERSION     DAZKMB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.293"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..511)
                     /locus_tag="ACG8V3_002970"
     CDS             complement(<1..511)
                     /locus_tag="ACG8V3_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0301405.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVL"
     gene            complement(587..733)
                     /locus_tag="ACG8V3_002971"
     CDS             complement(587..733)
                     /locus_tag="ACG8V3_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFQ0301406.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT          312 a          130 c           87 g          263 t
ORIGIN      
        1 ttaagacacc aaacacaaaa tgatcaaata cttgatattg atgagcaaca taagaaacaa
       61 ataaaccaat tgtaagtaaa taaatcatga atatagcagt aagtttagaa ctggactgtt
      121 caacaatcct atttatagct aattttctat ataaccaaat aaacatcagc aaaaacaaag
      181 aatcaagcat tatctggaat actacataac catacccttt tatcccttca aatgaaaaca
      241 tatacgtcat aaccatggaa gtaataatag atctgaaatc atcaataagc atacataata
      301 gtaatttatt taaaagcact atattagttt tcttcttttc ctttaatata aaataacttc
      361 cgattatagg tatagctgcg gaaacatcag taaataattc aacaaaaaaa gtgagtggta
      421 tcaataaaca tacaaaaaga tattttcttt tgttaagatc agtattagtc atataaaata
      481 agagaaaaaa gaaaaagttt cccacgaaca ttcttaatag ataagctttt gtatcgatag
      541 taatcaagag atcccctcct gattttagga tatcataaaa aaaattttag ttacactttt
      601 taaataatga aaaaacacat tccactaaat ttgatgccgg ttttccttga ggttttgttc
      661 cagcacctcc tttaattttt ttaagctctt gatctgaaca ttgtttagca tttagtctat
      721 ttttttcttc cattacgatt ccccctacaa actataaata cttttatgtc actgattcaa
      781 tatacctatt tt
//
LOCUS       DAZKMB010000284          777 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000284 DAZKMB010000000
VERSION     DAZKMB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.294"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(242..391)
                     /locus_tag="ACG8V3_002972"
     CDS             complement(242..391)
                     /locus_tag="ACG8V3_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301407.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          280 a          119 c          112 g          266 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacg
//
LOCUS       DAZKMB010000285          772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000285 DAZKMB010000000
VERSION     DAZKMB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.295"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..>772)
                     /locus_tag="ACG8V3_002973"
     CDS             complement(24..>772)
                     /locus_tag="ACG8V3_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0301408.1"
                     /translation="GAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVL
                     LVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAG
                     RFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGF
                     LQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEA
                     HVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          292 a          140 c           92 g          248 t
ORIGIN      
        1 aagcaatgaa agctgtcttc atattaattc tcctttttta aatacaagta agaaatgctt
       61 cccaaataat accagaaaac aattgaaatg acatcgcgct tattgaaaag tacatgagcc
      121 tccaccattt catctataaa aatagatagt ataaaaatta aaacaatacc aagaaacgca
      181 taattcactt tacttctaaa aaatgttttg agtaaactaa agatatttaa cacatagaaa
      241 gcatagatac atataaaagc aattatacca cttgatacaa gtacttgaag aaatccatta
      301 tgcatgtttc catgtgctct ttttagttca ttcacatctg tagtatcaag atctactttt
      361 ttcaaaaatt ttgcatcgct attcccatag aaatcggtct ctccataacc aaataacaaa
      421 gttggtctta taactttaag cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg
      481 gagttatcac tagaatgctg taatattact tctttttctg gaagtggagt tttatctgat
      541 tcatttgact ctataacttc tacttttcta tcaatcgata caggaattgt tgctaaaaaa
      601 tccttagcat aattagtacc aaaagaaaaa tataccaata gaactaataa tatgcttacc
      661 atccatttga tttttacagg tagtacttct ctaatgctct ttctaaattt tgaattggca
      721 aacaacaaaa gaaataatac taaaaaagaa caaattacta cctgggcacc tc
//
LOCUS       DAZKMB010000286          763 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000286 DAZKMB010000000
VERSION     DAZKMB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.296"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..421
                     /locus_tag="ACG8V3_002974"
     CDS             185..421
                     /locus_tag="ACG8V3_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0301409.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            440..655
                     /locus_tag="ACG8V3_002975"
     CDS             440..655
                     /locus_tag="ACG8V3_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFQ0301410.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT          247 a          106 c          182 g          228 t
ORIGIN      
        1 tgtctcataa ttccacaaaa actttgaaaa cagttcttga acactaactt tttcattcct
       61 gaatcaaatt tattttggaa attttactat tcaagaataa acttgataag tttcaggaag
      121 atttttactg ttaatcaaat acgtatgtta cagtagcacc gtaaaaaata ttatttaaag
      181 aagcgtgttt aaacatgcaa aatgtaaagg aactaagtat tgtggaaatg cacaaactgt
      241 tggtggaggc aataatgctt ggggaaaatt ggggcaagtt gtaggtggac ttaccacagg
      301 ggcagtaggt ggtgcaggtt taggaacagc aatttgcgga ccagcttgcg gtgttgttgg
      361 agggttatat ggagctgtcg ctggaggtgc tgcagctggc tgggatgctc gaaaaaaata
      421 aaaaaggaga ataagtagta tgcatattaa aaacacaaaa acaacattta tattatcttc
      481 agaagagttg aaaaatattc aaggtgggag tgccgtggga gttttaggca ctactttttc
      541 tggggcaaca gcaggagtaa aactttgttc agccggagga ccttatgcaa tagctgcttg
      601 tggtgtaggt ggagctttat taggtgctgg attcagcatg tggaccggtg cgtaaacaat
      661 taaaaaatat caaacctcaa gattggtttg tgtcgattac gaacataggt atgttcttta
      721 tcttttcaaa agtttggaat ggacagaact ttttactaaa gct
//
LOCUS       DAZKMB010000287          755 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000287 DAZKMB010000000
VERSION     DAZKMB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.297"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..652)
                     /locus_tag="ACG8V3_002976"
     CDS             complement(<1..652)
                     /locus_tag="ACG8V3_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0301411.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIK"
BASE COUNT          248 a          145 c           85 g          277 t
ORIGIN      
        1 ctttaataaa acttgttatt tttgaatgtt gcaaatatga aatatggaat atgatatctt
       61 cagatatata atccctttct gacggaaact tgatattgtg ctgttgaatt atctcagatg
      121 aatacagcgc atgccaaact gacattggca atataaaatc gactttataa ctcggctcag
      181 tgccaatcat tccaagcaac actttttcaa ttatagcgtt atttgtaaat atcgtactat
      241 catatcttgc tggctttgaa ataatattat tatcatcata atattcataa tatccagcat
      301 aacatgtgtc tgcattactc ctttttatac catttaacat tgcttcaata taattagctg
      361 caacaaaatc atcagaatca ataaaagcaa catattttcc ttttacatgt tcaagtcctg
      421 tatttcttgc caatcccaag ccagcattat ttttatgaat aatacgaatc cttgaatcct
      481 ttaatgcata gtcttcacat attttattag atgaatcctt agatccatca tccacaatta
      541 taatatctag gttcttatat gtttgattac acactgattc caaacaactt cctacatact
      601 tttcaacatt atatacaggt atgattacac taattagatc actcgaattc atccttctat
      661 cctttcactc acgttttctc aattagtcaa tgacggcatc aacgattttc ttaaattctc
      721 tgaaaaaaat agactctgat cttgatgatg tgaat
//
LOCUS       DAZKMB010000288          731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000288 DAZKMB010000000
VERSION     DAZKMB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.298"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(333..446)
                     /locus_tag="ACG8V3_002977"
                     /pseudo
     CDS             complement(333..446)
                     /locus_tag="ACG8V3_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          268 a          115 c          112 g          236 t
ORIGIN      
        1 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
       61 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
      121 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
      181 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
      241 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      301 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      361 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      421 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      481 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      541 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      601 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      661 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      721 atataaaaaa a
//
LOCUS       DAZKMB010000289          725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000289 DAZKMB010000000
VERSION     DAZKMB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.299"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACG8V3_002978"
     CDS             <1..>725
                     /locus_tag="ACG8V3_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0301412.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DAZKMB010000290          712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000290 DAZKMB010000000
VERSION     DAZKMB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.300"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..479)
                     /locus_tag="ACG8V3_002979"
     CDS             complement(<1..479)
                     /locus_tag="ACG8V3_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HFQ0301413.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     "
BASE COUNT          219 a          133 c          109 g          251 t
ORIGIN      
        1 gcaagcaatc ctagctcttc tttaatattc tcctggatca tttgagccag cttctttctc
       61 cgttgacttc ttgttccttc agtcgtaaaa agattcattg atctggtcac ttttaagatc
      121 gattcatcga tcgagtaaat caataaatct tcgtctgcta cataccttct gaaaatattg
      181 tttacctgca tatttcgctt aatatagaga ttcatgcgtg gtggaacgac atgtaacgtt
      241 ttaggaaaag gttgcggtag atctcgtggt cgactgacat tagaaatacc gtacttcttc
      301 tttgcagctg gtgaagaagc taaaatcaac cctgatcctg tattatccgc tcgactcatg
      361 acgactaatt cagtcgttaa tggatctaaa ttcctttcta tacattcgac acttgcgtaa
      421 aagcttttca tatcgatcaa aaagtaatca tttactggct cttttgaata atccatcata
      481 aataacactc cattcttcca cattatacaa acatacgttc gtttttgtaa agaagaatcg
      541 attaaatgta caaaaaaaga agcataggta agttatttcc ctacttcttc tgttgatttg
      601 taaaatcaac acattgtatc actttattaa atggatattt actattagtc ttgtgaaaat
      661 ttgaaaataa gtgtcgtatt atccagtttt gcttagttat attagaaaaa aa
//
LOCUS       DAZKMB010000291          700 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000291 DAZKMB010000000
VERSION     DAZKMB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.301"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..>700
                     /locus_tag="ACG8V3_002980"
     CDS             81..>700
                     /locus_tag="ACG8V3_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0301414.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITS"
BASE COUNT          225 a          100 c          138 g          237 t
ORIGIN      
        1 gtatttactt tttttgataa gtgatgtatt atttgggtgg aaaaagtaat cgattacaat
       61 aagcataaag gagaaaaaat atgggcaaaa gtagcaggaa ggacattgta tttgaaaaat
      121 ttggtatagt tgcgactaag ttggggaatc agatccattt aagaactttg cgagatgcgt
      181 ttgctacatt tatgccattt atgatgttag caggatttgt gacacttatt aattcagtga
      241 tcttagaacc aacaggattc atgggggaaa ttgtcgatac tagtacattg accacgttac
      301 aacaaattgg tacttcaatt gccaatggta cattaagcat aactacatta ttagttgtag
      361 cagcagtatc ttatcatatg tgtgcaagca gaaattatac aaatcatatt gcagcggtat
      421 tagttgctat ttcgacattt gttgtattaa cacctatgca gatgatgttt actcctgaaa
      481 atgcggataa agctgttgaa gtgacgggtg tgatacctat atcctataca ggagcttcag
      541 gaatgtttgt aggtatattt gttggattag cggctacaga attatttatt aaactttcta
      601 ataataagaa actacagatt cagctatccg gaaatattcc tcccgctgtt ttaaagtctt
      661 ttaatgtatt aatcccaatc attattacga ttacctcgtt
//
LOCUS       DAZKMB010000292          693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000292 DAZKMB010000000
VERSION     DAZKMB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.302"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8V3_002981"
     CDS             132..344
                     /locus_tag="ACG8V3_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301415.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>693
                     /locus_tag="ACG8V3_002982"
     CDS             506..>693
                     /locus_tag="ACG8V3_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0301416.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVL"
BASE COUNT          247 a          103 c          122 g          221 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct aga
//
LOCUS       DAZKMB010000293          656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000293 DAZKMB010000000
VERSION     DAZKMB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.303"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8V3_002983"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8V3_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0301417.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAZKMB010000294          646 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000294 DAZKMB010000000
VERSION     DAZKMB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.304"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>646)
                     /locus_tag="ACG8V3_002984"
     CDS             complement(<1..>646)
                     /locus_tag="ACG8V3_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0301418.1"
                     /translation="LLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREID
                     REKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVL
                     QRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIF
                     SEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLA"
BASE COUNT          183 a          115 c           68 g          280 t
ORIGIN      
        1 gctaattttg taaatgttac tgttccatta taattttcta aaacaacttt ctctatttcg
       61 tctagtgaaa ctttgaaaaa ttcttttctt tgattaattt tattaacttc ttgttttcta
      121 aaatgattat gcaatttagt ttccaaagac ggagcgtcct ctgaaaaaat catcgcatga
      181 acgtcaaatt caaatggaac cgatgcacta ctcaattctt ttattctatc cataggttct
      241 aatcttctag tcataccaat tttatatatg tcttcaccaa aagacccaat attactaatt
      301 acatacacat aaccggctct agtatttaat tctctttgta atacattttc acgtataact
      361 tctaattctt ttaatttttc ttccagttct tgaattttat ttgcatataa ttctttttcc
      421 acatctgcat tagctttttg catatatgcg attaatttgg aaatttcaga attgaattga
      481 cgttgttctt tatcaatacg tttcttttca cgatcaattt ctctacgaac tttttcctct
      541 tctatcatct gttcttttat tgctttttgt tgttcttttt cttcggtaat tttcactaaa
      601 taagagtaat gaagttctag ttcttgaagt ttaaactcta aaagac
//
LOCUS       DAZKMB010000295          621 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000295 DAZKMB010000000
VERSION     DAZKMB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.305"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..162
                     /locus_tag="ACG8V3_002985"
     CDS             <1..162
                     /locus_tag="ACG8V3_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0301419.1"
                     /translation="ISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHAN
                     EVLKIAEEK"
     gene            163..>621
                     /locus_tag="ACG8V3_002986"
     CDS             163..>621
                     /locus_tag="ACG8V3_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0301420.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNM"
BASE COUNT          235 a           74 c          116 g          196 t
ORIGIN      
        1 ataagttttg aagaaaacat tgttaaagaa atgcctgaat ctgctaatga tatgtatgag
       61 aaggagttga aatatttctt tgaaaacgta gtaacaggca aagaaaactg gaatgattta
      121 tatcatgcaa acgaagtatt gaaaatagct gaggagaaat aaatggaaat tttatttact
      181 atttgtggta gagctgggtc taaggggttt aaaaataaaa atttgaaata tttggcagga
      241 aagcccctag tccatttcgc tttagcaatg atagatatct atcaacaaaa acatcctgaa
      301 cataattgca cgacttgttt gaatacggac agtaatgagt tagtagaatt gacgactaag
      361 ttaaatccgt ctgtgaaaat tgtagaacgt aaacctgaat tagctacaga tactgtaggg
      421 aaaattgaag tgatacgtga ttcattctta caactagaaa gagaagataa tatatttgat
      481 ttaattatag atttagattt gacttcacct ttacgtgaag tttctaatct tgaagaatta
      541 atagatgaac atctaaaaaa tttagataaa gacgtctact tttcagttgt ttctgcaaga
      601 cgtaatccct attttaatat g
//
LOCUS       DAZKMB010000296          616 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000296 DAZKMB010000000
VERSION     DAZKMB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.306"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..166)
                     /locus_tag="ACG8V3_002987"
     tRNA            complement(82..166)
                     /locus_tag="ACG8V3_002987"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Leu,seq:caa)
     gene            complement(184..255)
                     /locus_tag="ACG8V3_002988"
     tRNA            complement(184..255)
                     /locus_tag="ACG8V3_002988"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Gln,seq:ttg)
     gene            complement(265..337)
                     /locus_tag="ACG8V3_002989"
     tRNA            complement(265..337)
                     /locus_tag="ACG8V3_002989"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(302..304),aa:His,seq:gtg)
     gene            complement(399..483)
                     /locus_tag="ACG8V3_002990"
     tRNA            complement(399..483)
                     /locus_tag="ACG8V3_002990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(447..449),aa:Leu,seq:caa)
     gene            complement(504..574)
                     /locus_tag="ACG8V3_002991"
     tRNA            complement(504..574)
                     /locus_tag="ACG8V3_002991"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(540..542),aa:Cys,seq:gca)
BASE COUNT          190 a          146 c          133 g          147 t
ORIGIN      
        1 tcgaattgat ttggaggtaa ttacataaaa acaaaaaaaa tatagcaact ctctataaga
       61 aagttgctat attgataatt ataccggcgg ccggggtcga accggcacgt cctcaacgga
      121 cactggattt tgagtccagc gcgtctgcca attccgccac gccggcaaat ttaaaaataa
      181 taactggggt agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg
      241 cttggctata ccccaagaat aaaaggcgaa tgatgggaat cgaacccacg aatgccggag
      301 ccacaatccg gtgcgttaac cacttcgcca cattcgccat cataaaagat gttatttaat
      361 ttctcaataa aaaactaaga tgaagacatc ttagtcaata ccggcggccg gggtcgaacc
      421 ggcacgccct caacgggcac tggattttga gtccagcgcg tctgccaatt ccgccacgcc
      481 ggcaagttga tttattagta ctaaggcggt aaccggattt gaaccggtga tgaaggtttt
      541 gcagacctct gccttaccac ttggctatac cgccattata ctattatcaa tctataaaat
      601 aaagaataaa ttatag
//
LOCUS       DAZKMB010000297          601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000297 DAZKMB010000000
VERSION     DAZKMB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.307"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..586
                     /locus_tag="ACG8V3_002992"
     CDS             <1..586
                     /locus_tag="ACG8V3_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0301421.1"
                     /translation="STGIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFT
                     DDNPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWH
                     IANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKK
                     VYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          206 a          109 c          122 g          164 t
ORIGIN      
        1 atccacagga atcaaatcgc atttatgggt aacacaaaat atttggggcg atcatacaga
       61 tccttgcggt tatctttctg aaatgggtgt aagtaaagaa aaactagcct atgatttggc
      121 tcatggtttt acggatgata atccaactac ttcggatgat aatccaacta cttcggagga
      181 taaaccagta attgatccaa ctcgagcagg tgctgcaaat tctacgctga cagatggaac
      241 aaattacgcc cacgttgatc agttcggaga aatcgaaaac gcaaacttgc atgtagctgg
      301 atggcacatt gctaattata aatacgagta tattttcatt atggactaca atactggaaa
      361 agaactagct agagtaagag ctgatgggat ttatagacca gacgtaaatc aagcttataa
      421 tactttagga aatgttggct atcatgtatc tttcaatatg cgtgatttcc ctaataagaa
      481 agtatacgtc atgatgcgtg cgacaaacga tccagaagga aatactaagg gtggagcaca
      541 agattttcat gacaagcgtt ggtatttaaa tattccacaa cgataaaaaa ttcccccctc
      601 t
//
LOCUS       DAZKMB010000298          584 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000298 DAZKMB010000000
VERSION     DAZKMB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.308"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..325
                     /locus_tag="ACG8V3_002993"
     CDS             <1..325
                     /locus_tag="ACG8V3_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0301422.1"
                     /translation="NKTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGI
                     CTAAFRANNLSCMIYGIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEH
                     LVLQH"
BASE COUNT          241 a           77 c           87 g          179 t
ORIGIN      
        1 gaataaaaca ttaggacaaa cgatacacaa taaaagatgt tatgtgatat taattaatca
       61 aaacctatca ccttctatga aaagatttgt attatggcat gaaatagccc acgttagact
      121 ccataaaggt atttgcacag cagcttttag agcaaacaac cttagttgta tgatttatgg
      181 tatagaagct gaagctaata tgtttgcaat tgaaatgtta actagaacaa tagatgaaca
      241 agatttatct gatatgagtc agttccaaat tattgaatat ttagggcttc ctcataatct
      301 tgaacattta gtattacaac attaaaaata aaaaagccca tgctttcaca tggactcttt
      361 cctcatttct gagtaaactc tacaataatt atatcaaaga aatgaggagt tttgaatgtt
      421 attattgatt ggacttattt tattaattat aggaatcact aaactgtcga aagctaacac
      481 aataaaaaaa gaagcttatg aattagaaca aaaaaatgat gaaaaactaa ataaattaca
      541 tgatagagaa agatttatat taagtaagga agaaacacta tcag
//
LOCUS       DAZKMB010000299          583 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000299 DAZKMB010000000
VERSION     DAZKMB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.309"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..>583)
                     /locus_tag="ACG8V3_002994"
     CDS             complement(38..>583)
                     /locus_tag="ACG8V3_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0301423.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          167 a          110 c          100 g          206 t
ORIGIN      
        1 aaatccactc cttttttcat gctctaaaat gatattttta cacaactgtt acggttgttt
       61 tacgaaattg gacgggactt tgccagttta atttctgttt tatccgttca ttattgtagt
      121 aatagatata gctatcgatg gctgatttta attcctcgaa acagttataa actttcccgt
      181 gaaaaatctc ttgttttaat aagccaaaaa aattctccat aagtgaatta tctaagcagt
      241 tccctttccg agacatgctt tgaaagattt tgtactcctt caatttgttt ctataagctt
      301 tcatttgata cgcccaacct tgatctgagt gaaacgtacg acgataggga caatcgttag
      361 tggtctggat agcttcctcc aaaccttcca ttaccgctaa tgcattgggc ttttcagaga
      421 tacgatagga taaaatctca ctattgaaca tatccataaa aggatctaga tacaatttct
      481 tttgacggat aatgcctttg gcatccactt catggtattt aaattcagtt gtatccgtcg
      541 tgattttctg atggcagata ctggtgtaga aacgacgatg gat
//
LOCUS       DAZKMB010000300          551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000300 DAZKMB010000000
VERSION     DAZKMB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.310"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..524
                     /gene="spxA"
                     /locus_tag="ACG8V3_002995"
     CDS             126..524
                     /gene="spxA"
                     /locus_tag="ACG8V3_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0301424.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          196 a           87 c          110 g          158 t
ORIGIN      
        1 taaaaaaatt atgaaaaaaa ggtagctatt taaaagttga ttatgtataa tagaaacaga
       61 tgggagatta ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt
      121 gcacgttgtt aacattatat acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt
      181 ggttgcaaga acatcagatt ccatttgttg aacgtaatat tttttcagaa ccattgaaca
      241 gttctgaatt aaaagcgatt ttacaaatga cagaagacgg aacagaagaa atcatctcga
      301 cacgttccaa agtattccaa aaactgaaca tggatttaga tgatctgccg ttacaagaat
      361 tattggaact tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg attgatgata
      421 aacgtctgca agttggtttt aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac
      481 gacaattaga attacgtcaa gcacaattga tggctggatt atagaaggat aatacgaaac
      541 ttggaaaaga c
//
LOCUS       DAZKMB010000301          540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000301 DAZKMB010000000
VERSION     DAZKMB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.311"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..147)
                     /locus_tag="ACG8V3_002996"
     tRNA            complement(74..147)
                     /locus_tag="ACG8V3_002996"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Ile,seq:cat)
     gene            complement(314..>540)
                     /locus_tag="ACG8V3_002997"
     CDS             complement(314..>540)
                     /locus_tag="ACG8V3_002997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301425.1"
                     /translation="GTCLNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEYVLGIS
                     KCLNVFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT          185 a           87 c          102 g          166 t
ORIGIN      
        1 tgaagaaaag ctttagcttg tataatactc ttcaaaaaat tctaacacag aacgattcaa
       61 ttggctacat taatgtaccc tgtaggactc gaacctacga ccggacggtt atgagccgtc
      121 tgctctgacc aactgagcta agggtagtgg ttgttgccac ataaagccat aaacaatcaa
      181 ccagtagaat gcgtggcaac aaacctgtta tcgcatatct tggagtgtga ctatttatga
      241 gtgatagtga agatatgcgt ctccatatat gtatggttga gacagataaa catgtatgaa
      301 aaacgttgtt aaatcaacgt ttttttattt tggtcaagga gagaaaagta gagaaataca
      361 aaattttttg cattgatttt gcattcaaaa aaacattcaa acatttagaa atccccaata
      421 cgtattcagg aaaatctatt ttaaggttta caaaaagaac ttttgttcgt acactctttt
      481 tgaggtgaac ttttatgctg atggaaggaa aaacacaatt atggtttaag catgttcctt
//
LOCUS       DAZKMB010000302          537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000302 DAZKMB010000000
VERSION     DAZKMB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.312"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..400
                     /locus_tag="ACG8V3_002998"
     CDS             143..400
                     /locus_tag="ACG8V3_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301426.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          231 a           67 c           75 g          164 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa attagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgacaaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagat
//
LOCUS       DAZKMB010000303          496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000303 DAZKMB010000000
VERSION     DAZKMB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.313"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..327
                     /locus_tag="ACG8V3_002999"
     CDS             <1..327
                     /locus_tag="ACG8V3_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301427.1"
                     /translation="KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQ
                     REGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRK
                     LSEVNN"
BASE COUNT          199 a           66 c           90 g          141 t
ORIGIN      
        1 aaaaaggcaa gtttaaaatc actaaaagat acatggcttg atttatcaga agataatcca
       61 tacagccaat tcttaattac tgtaatggct ggtgttaacc aattagagcg agatcttatt
      121 cggatgagac aacgtgaagg gattgaattg gctaagaaag aaggaaagtt taaaggtcga
      181 ttaaagaagt atcataaaaa tcacgcagga atgaattatg cggtaaagct atataaagaa
      241 ggaaatatga ctgtaaatca aatttgtgaa attactaatg tatctagggc ttcattatac
      301 aggaaattat cagaagtgaa taattagcca ttctgtattc cgctaatggg caatattttt
      361 aaagaagaaa aggaaactat aaaatattaa cagcctccta gcgatgccga aaagcccttt
      421 gataaaaaaa gaatcatcat cttaagaaat tcttagtcat ttattatgta aatgcttata
      481 aattcggccc tataat
//
LOCUS       DAZKMB010000304          495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000304 DAZKMB010000000
VERSION     DAZKMB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.314"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..165
                     /locus_tag="ACG8V3_003000"
     CDS             43..165
                     /locus_tag="ACG8V3_003000"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301428.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            138..269
                     /locus_tag="ACG8V3_003001"
     CDS             138..269
                     /locus_tag="ACG8V3_003001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301429.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
BASE COUNT          185 a           73 c           87 g          150 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catcgccgat tgtaaaatta
      361 agctagacaa ctaaaaaagt catttgtgct acactcaaaa tagttccgac caaagaatta
      421 taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg taataataga
      481 gtcttatttc aaaat
//
LOCUS       DAZKMB010000305          490 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000305 DAZKMB010000000
VERSION     DAZKMB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.315"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>490
                     /locus_tag="ACG8V3_003002"
     CDS             <1..>490
                     /locus_tag="ACG8V3_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0301430.1"
                     /translation="WTPDVIIGRKERSVSCGMRTLYRLFSKGIFDIDTLPMKGKRKPN
                     GHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVII
                     TIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADP
                     GTP"
BASE COUNT          159 a           94 c           92 g          145 t
ORIGIN      
        1 gttggacgcc tgacgtcatt atcgggcgaa aagaaaggtc tgtttcatgc ggtatgagaa
       61 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
      121 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      181 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      241 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      301 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      361 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      421 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      481 ctggaacacc
//
LOCUS       DAZKMB010000306          473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000306 DAZKMB010000000
VERSION     DAZKMB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.316"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>473
                     /locus_tag="ACG8V3_003003"
     CDS             <1..>473
                     /locus_tag="ACG8V3_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0301431.1"
                     /translation="TAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPE
                     EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPK
                     DTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          192 a           71 c          112 g           98 t
ORIGIN      
        1 accgcaggag aaggaacaag tattgaaagt ggaacaacag tctttgcagt gaaagacggc
       61 gtaagcttac cagaagataa acttccagtg ttgaaagcaa aagatggtta tacagatgcg
      121 aaatggccag aggaagcaac tcaaccaatt acagcagatg atacagaatt tgtatcaagt
      181 gcaacaaaac tggatgataa atctgacgct gacaaatata atcctgaagg tcaaaaagtg
      241 actacagaat tgaataagga accagacgca tttgagggaa ttaagaacaa agaagattta
      301 ccaaaagata ctaagtatac ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa
      361 aaagggactg ttgtagtgac atatccagat ggatcatctg atgaagttga agtagatgtt
      421 acagtgacag acaatcgttc tgacgccgat aaatatgagc caacagtaga agg
//
LOCUS       DAZKMB010000307          471 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000307 DAZKMB010000000
VERSION     DAZKMB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.317"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..233
                     /locus_tag="ACG8V3_003004"
     CDS             39..233
                     /locus_tag="ACG8V3_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301432.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          201 a           59 c           84 g          127 t
ORIGIN      
        1 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
       61 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      121 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      181 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      241 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      301 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      361 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      421 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt a
//
LOCUS       DAZKMB010000308          466 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000308 DAZKMB010000000
VERSION     DAZKMB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.318"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..287)
                     /locus_tag="ACG8V3_003005"
     CDS             complement(<1..287)
                     /locus_tag="ACG8V3_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0301433.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDP"
BASE COUNT          170 a           77 c           63 g          156 t
ORIGIN      
        1 tctggatcaa cgaaagtata ctttatatag tcataacgcc gatgatcgct tcgagcgtct
       61 agcacatcag tcacgatatc aaacaaaaaa tatacgtctt tcttcattct agttttcgca
      121 gcagggattt taaagtagtt cttattagaa tagtagagat taattaataa gctatcttcg
      181 attgctaaaa agaaaacttc tgaatcccac accttataaa aatctttgac aaatctattc
      241 gaaggatcaa atttaaacca taattgtgtt tttccttcca tcaacataat gttcacctca
      301 aaaagagtat acgaactaat gttctttttg taaagtagga atatcattga taatatggat
      361 ggaacatata tattaaaaag tgttcttttt gaatgctatt ttgaatgcca ttttcattca
      421 tttaacgaaa atctgcgaaa ataaaaaatg ctcaaaccct tataaa
//
LOCUS       DAZKMB010000309          449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000309 DAZKMB010000000
VERSION     DAZKMB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.319"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..270)
                     /locus_tag="ACG8V3_003006"
                     /pseudo
     CDS             complement(34..270)
                     /locus_tag="ACG8V3_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          146 a           76 c           77 g          150 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaataacgc cgtttgtata attaagcgag acaaataaaa
      301 aagccattta tgttacactc caattgtaat acccgctaaa gcatacaagg agagtgaaca
      361 taaatgactt atacccatct tacaccaaac gagcttgtaa tgatagaagc atattttcat
      421 caagaaactc cggttgctat cgttgcgaa
//
LOCUS       DAZKMB010000310          443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000310 DAZKMB010000000
VERSION     DAZKMB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.320"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>443
                     /locus_tag="ACG8V3_003007"
     CDS             22..>443
                     /locus_tag="ACG8V3_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0301434.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQA"
BASE COUNT          160 a           84 c           91 g          108 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggc
//
LOCUS       DAZKMB010000311          434 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000311 DAZKMB010000000
VERSION     DAZKMB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.321"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..416
                     /locus_tag="ACG8V3_003008"
     CDS             <1..416
                     /locus_tag="ACG8V3_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0301435.1"
                     /translation="LKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGII
                     LDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQG
                     LGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT          161 a           55 c           83 g          135 t
ORIGIN      
        1 atttgaaaag tttgttgata gcgaagctat accaagcaaa taacaataag atttgttgta
       61 aattcgaatg tccaagtact attattgaag ttcctgttcc aatatttgac tgtattaggg
      121 tgcttggaat tattctcgat aatgcaattg aagcatctat agagagtcaa gatagagaac
      181 tgtctctagc tatttaccga gatgataaac agatagaaat ttcgatatcg aatagttgta
      241 gagagatact actctctata gatactctaa tgaaaaaagg aataacaaca aaaaagggac
      301 atcaagggtt aggtttatct agtatagagg aaatcaataa aaagaatccc aatatgtttg
      361 tgaactatca aaacgatccg tatcaattta ctactcaagt tattttgttg ttttgaggga
      421 aggaggattt atga
//
LOCUS       DAZKMB010000312          425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000312 DAZKMB010000000
VERSION     DAZKMB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.322"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          123 a           75 c           55 g          172 t
ORIGIN      
        1 ttatttcttc acatttatta ggttatagtt taagaaagtt taaacattgc gcaatttaat
       61 tattttagct gtttcttttc ataaaaaaac atgttgtgac tctgagacaa atcatagtcc
      121 atagtgatgt ctctagagta actacgccta atattacgag gcatcatatt aatctatcag
      181 cactagtacc taaactagca tccctttcga atattttttc ttatataata aagtttgctt
      241 tcactcctct aatctcacca aatcgactgg cttgcgttgt gtgatactag gaactgattg
      301 gtttagaacg acttttctat tgtaatcata tttttcgcca tcctcgttgt cttctaatcc
      361 ttcaatgaat gatccctaat gtgtgtatta aataatttaa tttttttgtt cctatttatt
      421 taaca
//
LOCUS       DAZKMB010000313          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000313 DAZKMB010000000
VERSION     DAZKMB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.323"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..355)
                     /locus_tag="ACG8V3_003009"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACG8V3_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAZKMB010000314          418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000314 DAZKMB010000000
VERSION     DAZKMB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.324"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>418)
                     /locus_tag="ACG8V3_003010"
     CDS             complement(<1..>418)
                     /locus_tag="ACG8V3_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFQ0301436.1"
                     /translation="KQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAGLNNLF
                     TSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVFTLGLL
                     CIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLF"
BASE COUNT          160 a           75 c           46 g          137 t
ORIGIN      
        1 aaataaccaa tacgacaaaa cagtatttat caaatacatt aaatacaaaa ctgtcatatt
       61 taaatttcca ggaatcgaac ctttaactaa aaatctcaaa aaaggaatgc ataacaatcc
      121 taatgtaaac actatccatc caataattga aaagtacttt ttatatgcgt tcagtaaagc
      181 acaaatctct tttctattgt cttctgctat aggtttatac atacttacag ttagtgcagc
      241 acctattccc aattccgcta aatttaacat ttgaaatata gatgtgaaca ggttgttcaa
      301 tccagcatac tccattccca tctgataaat cataagtgta cgcataaaaa agggaatgat
      361 aatctgatat aacttaaaaa ttgttccagt aattatattc cttttagcat tttgttta
//
LOCUS       DAZKMB010000315          418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000315 DAZKMB010000000
VERSION     DAZKMB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.325"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>418
                     /locus_tag="ACG8V3_003011"
     CDS             <1..>418
                     /locus_tag="ACG8V3_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0301437.1"
                     /translation="YRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTK
                     GELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPI
                     TYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          140 a           77 c           71 g          130 t
ORIGIN      
        1 tatagagcgg tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc
       61 actcgcttaa gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag
      121 aagctcacca aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa
      181 ccagttagaa atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca
      241 acaaaaaaat cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt
      301 ccgattactt actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta
      361 tcggaagaag cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZKMB010000316          415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000316 DAZKMB010000000
VERSION     DAZKMB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.326"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG8V3_003012"
     CDS             complement(<1..339)
                     /locus_tag="ACG8V3_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301438.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAZKMB010000317          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000317 DAZKMB010000000
VERSION     DAZKMB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.327"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACG8V3_003013"
     rRNA            <1..>411
                     /locus_tag="ACG8V3_003013"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAZKMB010000318          403 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000318 DAZKMB010000000
VERSION     DAZKMB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.328"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..323
                     /locus_tag="ACG8V3_003014"
     CDS             9..323
                     /locus_tag="ACG8V3_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301439.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          128 a           52 c           62 g          161 t
ORIGIN      
        1 gatagctttt ggatttttac agtaatttta tcttaataat agcaatatta ttacttctaa
       61 atatttggtt ttttgataag agtagaaatg cagggatagg ttttagaacc aaacgaagta
      121 cgtcttctga aaaaaagtgg gtatattcac agactatttt ttacggaggg gttatttcaa
      181 tcagtttact ttcttcaaca ctttattctt ttaatgttat agacgtttcc atgtcaaatt
      241 ttatatctat tattggaatc cttatttctg caattattac acaactactt cttgtgtttg
      301 aagagaagtc aaaaaataat taaagcaatt atgcctttta gtacaggaat tattacttct
      361 tacaaatgta tagcgaatgt gaagcctgct gttttaattg ttc
//
LOCUS       DAZKMB010000319          401 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000319 DAZKMB010000000
VERSION     DAZKMB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.329"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..>401)
                     /locus_tag="ACG8V3_003015"
     CDS             complement(112..>401)
                     /locus_tag="ACG8V3_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301440.1"
                     /translation="TAVSRAYYSAFKKSDEYLKENYDIIYNGSSGKGSHQTVWNLFST
                     VKELNTLGIHNSGYRMLEKRKCADYIPNETITKTDMALMNREAEKIINKLT"
BASE COUNT          125 a           75 c           54 g          147 t
ORIGIN      
        1 agacgctcga agcgatcatc ggcgttatga ctatataaag tatactttcg ttgatccaga
       61 aagatacaaa gattaaataa aaaaataaca aacaaactcg cttgaaattc attaggtgag
      121 tttgtttatt attttttctg cttctcgatt catcagcgcc atatctgttt tcgtaatggt
      181 ttcattagga atatagtcag cacattttct tttctctaac attctatacc cactattatg
      241 tattcctaag gtattcaatt ccttaactgt tgaaaacaaa ttccaaacgg tttggtggct
      301 tccttttccg ctacttccat tataaataat atcataattt tctttcaaat actcatcaga
      361 ttttttaaaa gcactgtagt atgctcttga aacagcagtt c
//
LOCUS       DAZKMB010000320          398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000320 DAZKMB010000000
VERSION     DAZKMB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.330"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..>398
                     /locus_tag="ACG8V3_003016"
     CDS             65..>398
                     /locus_tag="ACG8V3_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301441.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSN"
BASE COUNT          175 a           56 c           60 g          107 t
ORIGIN      
        1 aaaagccttc gggctttttc ttttaacaat taataggaac gtatattcga aaggaataga
       61 aataatgtgg ttagaaaaaa taaatgacaa aaaatttaag tacaccgaac gttatacgga
      121 tcccttaact gaaaaaaaga gaaaagtgtc cgttacttta tctagcaatt cacgccaagc
      181 ttggaatcaa gctaacttac ttttgaatga aaaaatcgca gaaaaaataa aacgaaatga
      241 agaattacca ctaacttttg gagaattaaa aactaaatgg gatgaaaaat acaagccaac
      301 tgtaaaagaa agttcctatc gaacaactca ggtttatcta tcattaattt caaaatatat
      361 aaaagatgat gtacttgtaa aaaacgttaa tagtaatc
//
LOCUS       DAZKMB010000321          397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000321 DAZKMB010000000
VERSION     DAZKMB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.331"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>397
                     /locus_tag="ACG8V3_003017"
     CDS             <1..>397
                     /locus_tag="ACG8V3_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0301442.1"
                     /translation="TKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKK
                     ITVNNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNN
                     KISNMDKIFDVKFDLNKENSNGLGLYIVS"
BASE COUNT          178 a           23 c           60 g          136 t
ORIGIN      
        1 taacaaaaaa tatttctcaa atattatcag ttcattctat agaaaatttg aataatgatg
       61 aagaatattt gaaaataaat gatacattag aagacatatt aaaaaagtat gaaatattag
      121 caaatcaaaa gaaaataact gtcaataatt atatattaga cgagaatgtt tatataggaa
      181 aaacagcttt aaagattatt ctatctaatt tgattagtaa tgcggtaaaa tatactgatg
      241 taaatggggt aattaatatt gggatagtta atgattggtt atatatagag aattcatacg
      301 gtaacaataa aatttcgaat atggataaaa tatttgatgt taaatttgat ttaaataagg
      361 aaaatagtaa tggattaggt ttatatattg taagtaa
//
LOCUS       DAZKMB010000322          392 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000322 DAZKMB010000000
VERSION     DAZKMB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.332"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>392
                     /locus_tag="ACG8V3_003018"
     CDS             <1..>392
                     /locus_tag="ACG8V3_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0301443.1"
                     /translation="TYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDM
                     IGGVFWNYSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKV
                     FIPELFIIFVAIVFTLKVDFKVVIKILS"
BASE COUNT          125 a           43 c           65 g          159 t
ORIGIN      
        1 aacatattta acaattaaag attactataa agtattcgct ataattgaaa aattattctg
       61 gatttcactg ccactttgtt taattgaaag attcatggtc acctatccaa ctggaacaat
      121 tgtaggagat atgataggtg gagttttttg gaattattca ggttgtaata tgccattgaa
      181 tttaattttg tgtgtgacag ttactgttgt tacaatcgga tatttcaagg gagaaaaaaa
      241 tttcatccat tttattttat gtgtttgtgc agcacttttt atgtctgcat tagctgaatt
      301 gaaagttttt attcctgaat tatttataat atttgttgca atagtattca ctttaaaagt
      361 tgattttaaa gtagtgatca agatattatc at
//
LOCUS       DAZKMB010000323          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000323 DAZKMB010000000
VERSION     DAZKMB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.333"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8V3_003019"
     CDS             <1..>386
                     /locus_tag="ACG8V3_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0301444.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKMB010000324          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000324 DAZKMB010000000
VERSION     DAZKMB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.334"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8V3_003020"
     CDS             <1..>386
                     /locus_tag="ACG8V3_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0301445.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKMB010000325          383 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000325 DAZKMB010000000
VERSION     DAZKMB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.335"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..278
                     /locus_tag="ACG8V3_003021"
     CDS             <1..278
                     /locus_tag="ACG8V3_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFQ0301446.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
BASE COUNT          133 a           66 c           79 g          105 t
ORIGIN      
        1 aagaactcat caagctggct aataagggtc aagattatga acatctagca gatgagattg
       61 atgagctgag agacaagcga cagctccttt tagtagaaga tgcctccctc agtggcgaga
      121 acgagcgaat caatgagctg attgaattta tccgcaagaa caaattccgt accttagagt
      181 acgatgataa gcttgtaagg aagataatcc agagcgttac agtctatgaa gaacacttcg
      241 tcatatcctt caaatctggc atcgaaatgg aaatatgaaa accagaagta aatagcccat
      301 gattctacag tagagtcgtg ggttttcttt tgcttgctga ttataataat attttaataa
      361 ctattgttat tataaaccaa atg
//
LOCUS       DAZKMB010000326          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000326 DAZKMB010000000
VERSION     DAZKMB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.336"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACG8V3_003022"
     rRNA            complement(<1..>362)
                     /locus_tag="ACG8V3_003022"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAZKMB010000327          353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000327 DAZKMB010000000
VERSION     DAZKMB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.337"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8V3_003023"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8V3_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0301447.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          114 a           52 c           56 g          131 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atggaacaaa ata
//
LOCUS       DAZKMB010000328          351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000328 DAZKMB010000000
VERSION     DAZKMB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.338"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>351
                     /locus_tag="ACG8V3_003024"
     CDS             <1..>351
                     /locus_tag="ACG8V3_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFQ0301448.1"
                     /translation="LDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFR
                     KLQSVGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYK
                     KNRRNGTLFGFSVST"
BASE COUNT          118 a           72 c           71 g           90 t
ORIGIN      
        1 ttagatatct ggttacgaaa gaaacgggat acgcaagcag cctatgcttt cttaaaacga
       61 ctccataaac agtttggtga gccgaaagca attgtgaccg ataaagcacc ttctcttggc
      121 tccgccttta gaaagttaca gagtgtgggt ttatatacta agacagagca ccgaactgtg
      181 aagtatctta acaatttaat agaacaagac catcgaccta ttaaacgacg aaataaattt
      241 tatcaaagtc tccgtacagc ctcttccacg attaagggca tggagaccat tcgaggaata
      301 tataaaaaga accgaagaaa tggaacgctc ttcggctttt cggtgtctac t
//
LOCUS       DAZKMB010000329          344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000329 DAZKMB010000000
VERSION     DAZKMB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.339"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>344
                     /locus_tag="ACG8V3_003025"
     CDS             <1..>344
                     /locus_tag="ACG8V3_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFQ0301449.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DAZKMB010000330          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000330 DAZKMB010000000
VERSION     DAZKMB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.340"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..>321
                     /locus_tag="ACG8V3_003026"
     CDS             73..>321
                     /locus_tag="ACG8V3_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0301450.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLE"
BASE COUNT          106 a           59 c           60 g           96 t
ORIGIN      
        1 aaaaaaatat agaaaagaca ccctgaaatc ctttagaatt aatttggcta ccaattcata
       61 ggaggattca agatgtctca tactcagctt atcaaagata ccctaaatat tctagaccta
      121 aatatctatt tcgaggaaaa ttgtttaact aaagaaaaat acaaagggca aatttgcttg
      181 atttacaaag gaactcttct gtacaagcca gaagagtgtt cccattgtct ttgtgtcgtc
      241 ccctctagga tcattcgttg ggggacgaca accgttcggt tgttactgaa tgatgtgtct
      301 gagtatcgca cttatctgga g
//
LOCUS       DAZKMB010000331          312 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000331 DAZKMB010000000
VERSION     DAZKMB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.341"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..>312
                     /locus_tag="ACG8V3_003027"
     CDS             11..>312
                     /locus_tag="ACG8V3_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0301451.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYV"
BASE COUNT          110 a           34 c           58 g          110 t
ORIGIN      
        1 gagtataaaa atgaacaaac ataatagaaa aataacgaat aacaaagaac tgatcttgtt
       61 actaataggg atttggggta tctttttact ctttaatatg tatatgccga ctatgagagc
      121 ggatgacgta gtttatgcaa gtagattaga tgaacttggc tatttgggtg cttcgataga
      181 acattataag acatggagtt caagaattat tatagaacta ttcttaatgt tcttttctaa
      241 acactttatg ctatggaaat tattaaattc tacgattatg ttaggaattg tagtattact
      301 ttgcaagtat gt
//
LOCUS       DAZKMB010000332          299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000332 DAZKMB010000000
VERSION     DAZKMB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.342"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>299
                     /locus_tag="ACG8V3_003028"
     CDS             <1..>299
                     /locus_tag="ACG8V3_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0301452.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAZKMB010000333          295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000333 DAZKMB010000000
VERSION     DAZKMB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.343"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          128 a           48 c           48 g           71 t
ORIGIN      
        1 aattgtttgt aaaagaaacg gataaataat aaaaaagact gctcttccga gcagccagtt
       61 aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct tataaagcgc taccaaaaat
      121 ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg actggtaaaa attcacaaag
      181 aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa gagcttttta cctagaccaa
      241 ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa gggctacttg gcata
//
LOCUS       DAZKMB010000334          293 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000334 DAZKMB010000000
VERSION     DAZKMB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.344"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>293
                     /locus_tag="ACG8V3_003029"
     CDS             <1..>293
                     /locus_tag="ACG8V3_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301453.1"
                     /translation="YRESKGDLFIYSEFENARRYSERIKELGLFNDVVFVNHRKIGSS
                     FKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTKILFSNVYKKNPDVKLNYI"
BASE COUNT          110 a           36 c           44 g          103 t
ORIGIN      
        1 taccgagaat caaaaggaga tttatttatt tattctgaat ttgaaaacgc tagaagatat
       61 agtgagcgta taaaagaatt agggctattt aatgatgttg tttttgtgaa tcaccggaaa
      121 atcggttctt cctttaaacg taaaattaac ttgttaacta accataatca atttttctcg
      181 gatgatttat ccgtttatga tgaaattttt atacaggggg aaaattattt cacaaaaata
      241 cttttctcca atgtttacaa aaaaaatcca gatgtaaaac taaattatat aga
//
LOCUS       DAZKMB010000335          283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000335 DAZKMB010000000
VERSION     DAZKMB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.345"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..283
                     /locus_tag="ACG8V3_003030"
                     /pseudo
     CDS             1..283
                     /locus_tag="ACG8V3_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          109 a           57 c           56 g           61 t
ORIGIN      
        1 aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt cgagagtatg
       61 agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg ggctcattca
      121 aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga aagaaaaata
      181 agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc ggacgaaaag
      241 acaagtaatt gatgccaaca agcataaata ttccatatca gcg
//
LOCUS       DAZKMB010000336          272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000336 DAZKMB010000000
VERSION     DAZKMB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.346"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          113 a           30 c           49 g           80 t
ORIGIN      
        1 cctttaaggt gtcactaaaa attataaaaa atagtgttga cgcacttttg atgtcatgtt
       61 ataataacga agtcgtttgg atgagatagc taataaagct tgattttatc gaaaaaaact
      121 tcgaaaaagt tattgacaaa taacaataaa gatggtatga taatcaagtc gctgctaatg
      181 agtggcaaag acgtttagaa aaaataaaca aagaagttgt tgacattctc ttctgaatct
      241 gataagatta aagagttgtt aaaacaaaaa ga
//
LOCUS       DAZKMB010000337          248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000337 DAZKMB010000000
VERSION     DAZKMB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.347"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          117 a           30 c           37 g           64 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaa
//
LOCUS       DAZKMB010000338          238 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000338 DAZKMB010000000
VERSION     DAZKMB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.348"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..>238
                     /locus_tag="ACG8V3_003031"
     CDS             66..>238
                     /locus_tag="ACG8V3_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0301454.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT          103 a           36 c           37 g           62 t
ORIGIN      
        1 tgaaaatatt tatcataatt tttaatagaa aacactatta aagagaaaaa agagaggtaa
       61 atcaaatgaa aaaatcttta aaacttgcat cagcagttat attaatgtcc caactcctta
      121 tagcaacacc tttccatgtg ttggcaagcg aaaattcaac atacaaaatt gcaaaagaaa
      181 gaaccgagac gactgaaatg agtaattccg atgtaacagc agagagtagt gaactttt
//
LOCUS       DAZKMB010000339          232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000339 DAZKMB010000000
VERSION     DAZKMB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.349"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>232)
                     /locus_tag="ACG8V3_003032"
     CDS             complement(<1..>232)
                     /locus_tag="ACG8V3_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301455.1"
                     /translation="LLNDVSNYKTILRINKQRFKCKHCGKTFLAEDSVSDRRCSIARR
                     VKQAILELLSEPLSMSLIARMKHISPTTVIRIL"
BASE COUNT           70 a           42 c           53 g           67 t
ORIGIN      
        1 aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcgatca aagacataga
       61 aagaggttcg cttaatagct caagaatggc ttgtttgact ctgcgagcaa tggagcagcg
      121 acgatcacta actgaatctt ctgctaaaaa agtctttcca cagtgtttac atttgaagcg
      181 ctgtttgtta atccgcagaa tggtcttgta gttactgaca tcattcaata ag
//
LOCUS       DAZKMB010000340          231 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000340 DAZKMB010000000
VERSION     DAZKMB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.350"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           89 a           20 c           44 g           78 t
ORIGIN      
        1 aatatgtctt taaaaatgga atggttgcaa ttcttagcga tatatacgat gtaattgtcc
       61 tcttaaaaaa gtatggaaaa ataattgtat tgggtgttat gtataattca tggtgaactg
      121 agactaatga gaagaaaaat tcatattgtt atgagtgaga ttgtatcaga tgagaatgat
      181 aaattaaata ggtgcataaa agcattacac ttaaaataca ttgttgctta c
//
LOCUS       DAZKMB010000341          231 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000341 DAZKMB010000000
VERSION     DAZKMB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.351"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>231
                     /locus_tag="ACG8V3_003033"
     CDS             <1..>231
                     /locus_tag="ACG8V3_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301456.1"
                     /translation="SLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLG
                     RCRTCIQKAVRYASFNKEFDLNSYLSKILMND"
BASE COUNT           86 a           22 c           42 g           81 t
ORIGIN      
        1 ttcattgaca cataatttta gagataatga gttcgattgt aatattgaat tagttaaaaa
       61 aattgaaggc gatttaagct atatattaga aaatgagata tttgtaaaca aaacaaggag
      121 acttttgttg ggacgctgtc gtacttgtat tcaaaaggca gttagatatg ctagttttaa
      181 taaagaattt gatttaaact cctatttgtc taagatactt atgaacgatg a
//
LOCUS       DAZKMB010000342          226 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000342 DAZKMB010000000
VERSION     DAZKMB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.352"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..>226)
                     /locus_tag="ACG8V3_003034"
     CDS             complement(50..>226)
                     /locus_tag="ACG8V3_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0301457.1"
                     /translation="NLYYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYD
                     YLKYTFVDPERYKD"
BASE COUNT           65 a           44 c           39 g           78 t
ORIGIN      
        1 agtatggtga taaaatccgt tccgggctac cttttttagg tagcctactt taatctttat
       61 acctttctgg atcaacgaaa gtatacttca aatagtcata acgccgatga tcgctccgtg
      121 cgtctggcac atcagtcacg atatcaaaca aaaaatacac atctttcttc attctggttt
      181 ttgttgccgg aattttaaag tagttcttat tagagtaata gagatt
//
LOCUS       DAZKMB010000343          224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000343 DAZKMB010000000
VERSION     DAZKMB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.denovo.353"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACG8V3_003035"
     CDS             <1..>224
                     /locus_tag="ACG8V3_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFQ0301458.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZKMB010000344         9993 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.guided.105, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000344 DAZKMB010000000
VERSION     DAZKMB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.guided.105"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..1025)
                     /locus_tag="ACG8V3_003036"
     CDS             complement(153..1025)
                     /locus_tag="ACG8V3_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0301459.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1153..1722
                     /locus_tag="ACG8V3_003037"
     CDS             1153..1722
                     /locus_tag="ACG8V3_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0301460.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1789..2526
                     /locus_tag="ACG8V3_003038"
     CDS             1789..2526
                     /locus_tag="ACG8V3_003038"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0301461.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2757..4025
                     /locus_tag="ACG8V3_003039"
     CDS             2757..4025
                     /locus_tag="ACG8V3_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0301462.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4512..4976
                     /locus_tag="ACG8V3_003040"
     CDS             4512..4976
                     /locus_tag="ACG8V3_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0301463.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4960..5589
                     /locus_tag="ACG8V3_003041"
     CDS             4960..5589
                     /locus_tag="ACG8V3_003041"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0301464.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5943..7421
                     /gene="msr(C)"
                     /locus_tag="ACG8V3_003042"
     CDS             5943..7421
                     /gene="msr(C)"
                     /locus_tag="ACG8V3_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0301465.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7468..7914
                     /locus_tag="ACG8V3_003043"
     CDS             7468..7914
                     /locus_tag="ACG8V3_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0301466.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8113..8628)
                     /locus_tag="ACG8V3_003044"
     CDS             complement(8113..8628)
                     /locus_tag="ACG8V3_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0301467.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8745..9947
                     /locus_tag="ACG8V3_003045"
     CDS             8745..9947
                     /locus_tag="ACG8V3_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0301468.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3189 a         1750 c         2074 g         2980 t
ORIGIN      
        1 gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata aaaggaagga
       61 acaaattaag aaacttatgt tatttaatca ttggatcttt tgcttaactt attcgttgtt
      121 aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt taggacatct
      181 aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag attcagtgaa
      241 gcttgtaatg gcggatcgag tgggatgaat actttttccg tcccataagt attgatgatc
      301 ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc attgtacaat
      361 aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca ttcagctagt
      421 tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga
      481 tggatgactg tttgctttgc taaaggatga ttccgatgta tcaaaagtcc ccaacgatct
      541 tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat tacgagtcca
      601 aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc gctatataaa
      661 tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga aatgaaagag
      721 attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc gtcatttgtt
      781 aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga ataaagatat
      841 cgaccagaat ccgtcaactg tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt
      901 tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg cagagatttt
      961 gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag tatacgaata
     1021 tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta aaaggaatag
     1081 ttaagtatgt tttataagta ttagacatca taagccgtcc gttataaagt aaggataaaa
     1141 ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga gattcgagta
     1201 acggatccgc tctttgaagt gatccatcag atccaagcgg aaaacgaaga gcctttagca
     1261 gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga aaatatcatt
     1321 agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa
     1381 catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt
     1441 ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat cacggtgaac
     1501 catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca catcaagaaa
     1561 aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg cgagaatgcc
     1621 attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat cgttgcagga
     1681 atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag atgaaataag
     1741 agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat gaatgattta
     1801 tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc aacaacgaag
     1861 ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga tcgcctaaaa
     1921 gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga tgtaagagat
     1981 tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg aatcgatgta
     2041 ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca ccgtgatgaa
     2101 tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc cgccgtactt
     2161 ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt ggcgggacat
     2221 gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg
     2281 gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat ctctccagga
     2341 gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct tgaacttcat
     2401 gaagcgcaaa aagaatgggg attaacatct gaagatatag cttctgctgt agcatttgcg
     2461 atagaaacgc cagaccggat gtctgtcagc aacatgatta tccgtccgac gacacaggaa
     2521 gtataatcca taacggataa tatccagaga gaattacttg ttaaatagtt attcaaacga
     2581 ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt ttatgcagaa
     2641 aaagcaaatt tattaaaaag aaacaaatgt tatttaaata tggacattga cttgctttta
     2701 cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga aaaaagatga
     2761 aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt gccaacacaa
     2821 cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt tcttcagaaa
     2881 caggaattga aaacgataca gaaagtactt tgcagtcagc tactgtctca gaaaatacaa
     2941 tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa gacagtacat
     3001 cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa ttgacagatg
     3061 ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac tatactggct
     3121 atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat agtattgcct
     3181 caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg attgctcaag
     3241 ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat tacaatctat
     3301 ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg gaatatgtga
     3361 atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac aaagaatcat
     3421 tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt tactactatt
     3481 ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg ctaacaggtc
     3541 gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa acctataatc
     3601 tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg tataaccgtc
     3661 atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg ccaactgtag
     3721 gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct gtctaccgtt
     3781 tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag atcaactctt
     3841 taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt tccaagccaa
     3901 tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta
     3961 aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga ttttatggtt
     4021 attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag ccaaacaaaa
     4081 acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag tttttgaaca
     4141 ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt actgtttaag
     4201 catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt tcggaaattt
     4261 ttggcggtgt aaaattatct actgcagaag agctatcttc tatagtttga taagaatcaa
     4321 taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc tacaaaaaat
     4381 ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct gattctttga
     4441 cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca attattagaa
     4501 aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc aaattagccg
     4561 atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact agatacgaga
     4621 tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc
     4681 agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt
     4741 cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc
     4801 agaagaaaat caagcattgt gaaatggacc ataaagatac aggttccgtg ctatccccgg
     4861 ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg aatacccaac
     4921 tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa aaatgatttt
     4981 tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata caaaatccca
     5041 cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc tgtcaatatg
     5101 aaaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg
     5161 atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat ttttggcgat
     5221 atgcaatgtt acgaatatgg cgaagaaatc tacaaccaag cttacgaatc agggaagatg
     5281 ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa gagtttttca
     5341 agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat gcaattcatg
     5401 ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga agcagatcag
     5461 ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat tttatcaata
     5521 ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa gatcactatg
     5581 ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga atcgtgtgtg
     5641 gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac
     5701 ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt catggatgga
     5761 gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga tttggtgatt
     5821 tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt tgcaaacaga
     5881 tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg agtgtaaaac
     5941 atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc aaccagctag
     6001 aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc ataggagaaa
     6061 atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt cccgatcatg
     6121 ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat ctcgctgaag
     6181 caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa gaaacttcag
     6241 agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt ctttcttctt
     6301 atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa agtattgatc
     6361 gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt catgatcgct
     6421 attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa atccgagagt
     6481 tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag actcagctac
     6541 gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc caagaaaaga
     6601 aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac
     6661 tggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc caaaagaatg
     6721 cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg cagcagatga
     6781 aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat cccatcatgg
     6841 gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt gattttcagt
     6901 tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat
     6961 tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta agctttcaag
     7021 tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt tttgtcatga
     7081 gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta gggtttgctc
     7141 aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc cgtctgacga
     7201 ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg actaatttta
     7261 ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg ggaacgattt
     7321 tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt tatgaaataa
     7381 aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac gatgattttc
     7441 gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg aataattttt
     7501 acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt atggctgcat
     7561 caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt ggtggctttc
     7621 attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta
     7681 aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt tttaggaatg
     7741 gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga tcaattattc
     7801 tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc
     7861 atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga atgatggaac
     7921 actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga atgcagttct
     7981 agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct ttttcttttc
     8041 caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt ctagaaaggt
     8101 tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct gacaccattg
     8161 gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc caaattcttg
     8221 tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga ttttgttcag
     8281 ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc
     8341 atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct ctttgatcca
     8401 ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt gagagtgctc
     8461 gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg ttgtatagat
     8521 ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat acccagaacg
     8581 tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa aaatcctacc
     8641 tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca tgtgcatatt
     8701 gacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa atattggaga
     8761 atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag
     8821 ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac ccaatcttaa
     8881 aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta tttactagtt
     8941 acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt cggatcggtt
     9001 tgaaaaaaac attgatgaca ggattgttta tcatcgttgt attcgctggt cttggcggat
     9061 tatctgggaa tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt
     9121 tatttgtctc tactgcttta gcaacgatcg tcagtatttt agttggtaac acagaaaaag
     9181 caatcatgat gtatgaagca gcacttggct gcggaatggc tgttggccct ctagtcggtg
     9241 gggtcttggg cagtggttca tggcgttatc cattttttgg ggtagcattt ttgatgtttt
     9301 gcggttttat agccctagcg gtacttttac ctgaaactga gaaaccaaag agaaaagtag
     9361 gcctttggga aggtgtcaaa gcgttaagca atcataaact tcgttcagtt ggactaatcg
     9421 cattgttata taatttcgga ttctttactt tacttgcgta tgcgccattt ttattgattg
     9481 gctattctga attagaagtc ggttttgtat tttttggctg gggtgtgctt ctggctattg
     9541 cttcgatttt cgttgcaccg gctcttgagc ggaagtttac gacttatcgg acaattttag
     9601 cagcatttgt cttattcatt atttgtttgc tgttattagg agctggaact tctatgccag
     9661 tccttgtacc gatcagcatt gtgttagcag gatttttcca aggaattatc aatacactgt
     9721 tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg tccagctata
     9781 gttttgtccg ctttttcggt ggagctttag caccatttat tgcaggtgtg attggtgaac
     9841 atattggtga aagctatgcg ttttattttg caggtgccat tgttcttatc agtgtatgtt
     9901 gtatcattta tcaccgtaat tattttgcta caaatgaaga agaataagaa atcagaggtt
     9961 gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DAZKMB010000345         8326 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.guided.116, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000345 DAZKMB010000000
VERSION     DAZKMB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.guided.116"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..1170
                     /locus_tag="ACG8V3_003046"
     CDS             49..1170
                     /locus_tag="ACG8V3_003046"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0301469.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1428..2930
                     /gene="eat(A)"
                     /locus_tag="ACG8V3_003047"
     CDS             1428..2930
                     /gene="eat(A)"
                     /locus_tag="ACG8V3_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0301470.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2997..3524
                     /locus_tag="ACG8V3_003048"
     CDS             2997..3524
                     /locus_tag="ACG8V3_003048"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0301471.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3735..4796
                     /locus_tag="ACG8V3_003049"
     CDS             3735..4796
                     /locus_tag="ACG8V3_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0301472.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4799..5695
                     /gene="murQ"
                     /locus_tag="ACG8V3_003050"
     CDS             4799..5695
                     /gene="murQ"
                     /locus_tag="ACG8V3_003050"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0301473.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5709..7691
                     /locus_tag="ACG8V3_003051"
     CDS             5709..7691
                     /locus_tag="ACG8V3_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0301474.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7756..8202)
                     /gene="nrdI"
                     /locus_tag="ACG8V3_003052"
     CDS             complement(7756..8202)
                     /gene="nrdI"
                     /locus_tag="ACG8V3_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0301475.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         2763 a         1451 c         1864 g         2248 t
ORIGIN      
        1 aaaaaatgaa aaaaagtgtc ttacttaaga agaaagtgag ggatacagat ggatattttg
       61 gcggcaattg attcatttaa aggaagtgct tcttcatttg aactaaatca ggcagttcta
      121 aatggctggg aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa
      181 aatactgcag aagcaatcta ttacgcgctc aggggaacat ggcgtaccat tgatgggatg
      241 gatctcttat tccgtcagcg aaaaatcagc tatttattga caacttggca aggaaaaaac
      301 accgcagtca tcgaatctgc acaagcattg gggttagatc tacttgataa accaacggat
      361 caaactatcc gacttgcttc cagttatgcc ttaggagaac ttattaaaga tgcactgctt
      421 caagaagtag aacaaattat tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta
      481 ggattactcc aatccttggg tgcttcttta gaagggtaca cagaaggaaa tcctttatta
      541 tctactaaaa aacttcattt tgatcgaaga aacgttcttg tttctaaagt aaaattgatt
      601 atcgctgcag atgttaccaa tccatataca ggtcaacaag gagctgcttg gcaatttgga
      661 agacaaaaag gaggaataga tacgacgcta gctttcttag ataaacaagc aggaaagatt
      721 gctcagcaaa taaaagattt ctgggaggtg gatttagatg atattccagg aagtggagca
      781 gcaggcggta ttggtggagc cctttttttg ttgggagcga agatggtttc tggtttcgat
      841 ttagtcagcc aaatcactgg attggaaaaa aagatcgaac aagcagatgt catcattaca
      901 ggagaagggc ggatcgatca gcaaacgatc cacggaaaag ttccatacgg tgtagcagtg
      961 cttgcaaaaa aatatcgaaa accaataatc gctatttgcg gaagtagaga ccaagagcta
     1021 gaaggacttt ctgattttct cccaattgtc ttaagtattc atttgggtcc aataggttta
     1081 gaagaagcaa tggaacacag gcaaacatta gaaaaaacaa aaatcttagg acagacactt
     1141 agcagatttt tcaccatcct cccgaagtag ttgacaaaat cattttcttt atgctatcgt
     1201 aatttcaaat ataggacaaa gtgagggatc cgtgcattga aaaattaagt cgattgatga
     1261 aagctgcatt cagactgtac cttgtggaga caaggaagca gtgtgcagat tttcgggatt
     1321 ggcttggttt tttcgatgta tgggtctttt tgtgtgtatc caacccttgc atcctcttta
     1381 gccagttctg aaaataggac tggtcttttt ttacgaggtg atggaaaatg tctaaaatcg
     1441 aaataaaaaa tctgacattc ggctacgaca gccaaggcac attattattt gaacaagcaa
     1501 atctaaattt tgacacacaa tggaaactag gacttatcgg acgaaacggt cgaggaaaga
     1561 caactttact gaatattcta caaaacaaac taccttatca agggcaagta atccatcagc
     1621 aagaatttgc ctatttcccg caacagacaa aagataaaga acgtttaacc tattacgtgt
     1681 taaatgatat tacggatttt gagatatggg aaatcgaaag agagctccaa ttgatgcaaa
     1741 cagatcctga aatcttatgg agagaattca gcacactatc ggggggagag aagacaaaag
     1801 tcctactggc acttttattt gtggatgaca ctcatttccc gttaatcgat gaaccaacga
     1861 atcatttgga tatctctggt agaaaacaag tagcggctta tttgaaaaag aaaaaacaag
     1921 gcttcatcgt ggtcagccat gaccggggat ttatcgatga agtagtggac catgttttag
     1981 caatcgaaaa aagtcaactg gaactttatc aagggaattt ctctatctat gaagaacaga
     2041 aaaaacttcg tgatgaattt gaaatggctc aaaatgaaaa attgaaaaaa gaagtcagta
     2101 ggctaaagaa aacagcagct gaaaaagccg aatggtctcg ttcccgagaa ggagataaaa
     2161 caaagaaaca agtcggattc atcgatactg aatctagacg agtgaataaa ggagcagtgg
     2221 gtgctgatgc tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg atggagaccc
     2281 agatcagcga gaaagaaaaa ctattaaaag atatcgaata tatcgattct ttgacgatga
     2341 atagccaagc gtctcaccat aagcgacttt taagcgtaga agatcttcaa ttagggtatg
     2401 aaaatctgtt attcgagcca attcatttta caatcgagcc tcatcagcgg gtggcgattt
     2461 caggtcctaa cggtgcagga aagtcatcca ttatccatta tcttctgggg gcattcaacg
     2521 gcaaggttat aggagaaaaa taccagccaa aacatctgag cattagttat gcaagccaaa
     2581 attatgaaga caatcgagga acgttggcgg aatttgcaga gaaaaaccaa gtagactacc
     2641 aagcattttt gaacaacctc cgaaagcttg ggatggaaag agatgttttt cataacaaga
     2701 tcgagcagat gagtatgggc caacggaaaa aagtggaatt ggctaaatct ttatcacagc
     2761 cagctgaact atatacatgg gatgaaccat tgaattattt ggatgtcttc aatcaagaac
     2821 aattagaaca actgatcttg aacgtgaaac ctgccatgtt actagtggaa catgatcaaa
     2881 ccttcctgga taaagtatct actgagatta tttctcttga gagaatctaa cccttgtaat
     2941 ctcaagaaca acaaggtatg gttaagggga actttgccaa cgaaggagag aatcacatgc
     3001 tttatgtaac gagacacgga gaaacaactt ggaacgcaca aggattagtt tgcggacggg
     3061 cagatgtgcc gctgactgaa aaaggacaga tgcaggcaca gaaactagca gaaaaggtag
     3121 tagatttgcc tgttccaatc acgaaaatca tccattcccc tcttcaacga gcaagagata
     3181 cagcacaagc agttgctgat cgtctcagct tgccgctgac cgttgatgaa cgattggtag
     3241 aaatggattt tggggattat gatggactgc caagcaaaga tgagaacttt caaaaagcac
     3301 gtttagcgtt cgctgtacga ttcccaaatg gggagtcagt cttagatgtg tatgcacgga
     3361 tcgttccttt attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg ttagtttgcc
     3421 ataatgcctt gataagagtg atcaatgctt attttcatcc gatgcctaat gaggggttct
     3481 ttacattcat ggtagataat acagaattgg tttcttatga gtaattatgt ttttgtgtta
     3541 tttaaataaa atatttcaag gcactgcttt tgcttagcag tgcctttttt aggttatgac
     3601 aattcttttg ataatataaa ctcaaccttt agaaatgaaa tttaattatt ttaataaaag
     3661 tattgaaatt ttgtttcttt agatatatac tctagtcata gaaatgaaga cggatacaga
     3721 aaaagaggga taatatgttt ggcttttcaa tatttatgaa cgattctttg tcagaagaga
     3781 agaaacagta tatccgccaa atggcatcca atggctttgt cggtattttc acttccatgc
     3841 atataccaga agatgatgtt tcggcatatc aaaagcgatt gatcgaacta ggaagcgaag
     3901 cgaaaaagaa taaactagaa ttgatggtcg atatttcagg agaggcattg gaacgagcgg
     3961 gtttttcatt agaagatcta acgcctttga aagcaattgg tgtaacaggt ttaagaatgg
     4021 attatcatat ttccaatcag cagattgccg actggtcaca tcaattgaaa atcagtttga
     4081 atgctagcac gatcacgcct aaagatatcg atgaattgaa agaagcagaa gctgatttct
     4141 cacagattga agcttggcat aattattatc cacgtcccga aacagggctg gacaaagaat
     4201 ggtatcagaa aaaaaatcaa tggctcaaaa ttcaaggtct ccttgtccaa ggttttgtac
     4261 caggagatac agagttgaga ggcccactat atcaaggatt gccaactttg gaagaacatc
     4321 gagggatcca tcctcttgca ggcgctctag atctattagc aagtaacacg gatattgttt
     4381 acatcggaga tgcaggtctg tcagaaaacg ttcaggagca atttgcttct ttccaaaaag
     4441 aacagacagt cttgctccat acggaacctg tagatgaaga attttacgaa tatatattag
     4501 ggaagcatac gaatcggcaa gacgatgcaa gagatgtcat acgaagcgta gatgcacgtt
     4561 ttcgagagat tccgcctatc ccagcaagaa atacagctac gcgtatgaaa gggtctatta
     4621 ctttagataa cgaaaagtat ttgcgctata tgggtgagat ccaattaacc aaatatgatt
     4681 tgccagcgga tgaaaaagtg aatgtagtag ctaaagttat caaagaagag ctaccattga
     4741 tcaaccaaat caaagctggt atgaactatc aatttataag aaaagaagga agataaaaat
     4801 gagcaaaatc aatttagaga acctaacaac ggaacgcaga aaccaagaaa cttttggatt
     4861 agatgagatg agtatttctc aagccgttca actaatgaac aaagaagatc agaaagtggc
     4921 cgctgcggtt gaaaaagaac tgacaagtat cgaaccagtg attgctaaca cgattgagtc
     4981 ctttaaaaaa ggtggacgtc tgatttatat gggagccgga acaagtggaa gactaggtgt
     5041 gttagatgct gctgaatgcg taccgacatt cggtgtagaa ccggaaatgg tcataggtct
     5101 aattgctggt ggagaagaag caatgactgt agctgtcgaa ggggccgaag attcttatga
     5161 gctagggcaa gaagatctaa tcaagctgaa tttgacaaaa aatgatatag ttattggtat
     5221 cgcagctagc ggtcgtacac catatgtgat cggtggatta gattacgcaa aatcgatcgg
     5281 tgctgtcaca ggaacgattt cttgcaataa acaagccgaa atcagtaaac acgcggattt
     5341 cccaatcgaa gtcgatgcag gtccagaatt tttgacaggc tctactcgat tgaagtcagg
     5401 aacagcacaa aaacttatct tgaatatgat ttctactatc agtatgattg gtataggtaa
     5461 agtattcaat aacttgatgg tcgatgtcaa accaacaaat gaaaaactaa tcgaacgctc
     5521 aaaacgaatc atcatgcaag cgacagaagc tgattacgaa acagctgaaa agtactttga
     5581 agaagcagat caaaatgtta agctggctat cgtcatgatt ttgacaaata gtgataaaga
     5641 ggaagctcaa gcaaaattag ctgaagcaaa cggatttgta aaagggacct tatgaggggg
     5701 aaaagaccat ggcaaaaaac gaactgactt tggaagaact ggcaaaacaa atttataaag
     5761 gggcaggcgg catgggcaat gtagaaagca ttgtccactg catgactcgt gtgcgtatga
     5821 agatcatcaa tgatgatctt gtaaatgtct ctgaattaaa agcgattcca ggagtgcttg
     5881 gtgtagtaga agatgaacag cttcaagtca tcatcggacc tgggaaagtg aacaaagtag
     5941 cacagcaaat ggttgatttg gcaggcgtga aattaggaga aaaactttct tctgtaagta
     6001 cagctacagc aaattcatca gcatctggaa aagaccgtgt aaacgaacgg gcacaagaaa
     6061 tgaaagcggc acaaaaatcg aaacaaaaac catctaaaat caaatcggta ttaaaagaca
     6121 tttctaatat ctttgtgcca atgattcctg catttgtcgg tactgggatc gtggcaggga
     6181 ttgccgcagt tttagcaaac ttagtcacag ccggaacact tgatgcagca acatggcaac
     6241 aatatatcga tgtgatgaac atcttgaaaa acgggatgtt tagctaccta gttatctata
     6301 caggtatcaa tgctgcacaa gtttttggtg caacaccaac tcttggtgga gttatcgggg
     6361 ccgttgtgat gctgacaggg atgaatccgg aagcaccact gaaaaactta tttactggtg
     6421 aagctcttgc agctggacaa ggtgggatcc taggtgtcat cttcgctgtt tggctattgt
     6481 caatcgttga gaaaaaactg cataaagtag tacctgatgc ggtagatatc atcgttacac
     6541 caactctttc attgattgcc atcggtctga ttgaaatttt cttcatcatg cctttagcag
     6601 gtttcatttc tgatggcatg gtcggtggga tcaactgggt acttggcgtt ggtggagcat
     6661 ttgccggctt cgttctaggc acattattct tgccgatggt tatgttcgga ctgcatcaaa
     6721 ttttgactcc aatccatgtg caaatgattg atgaaactgg tcgtacgcta ttgcttccaa
     6781 ttcttgcgat ggctggtgca ggacaagtgg gtgcagcatt ggcattatgg attcgctgca
     6841 aaaaagataa agaattaaca gaaatgatca aaggagcttt accagtcggg atcctaggaa
     6901 tcggcgaacc attgatttac ggagtaacat tacctctagg acgcccattt attactgctt
     6961 gtatcggtgg aggtatcggc ggggcagtga tcggaatgat cggaaatgtc ggggctatcg
     7021 ctattggacc ttcaggagcc gctttgatcc cattgatctc tgacggtaaa tggttaggct
     7081 acatccttgg attattggca gcttatgctg gtggcttcgt tgctacattc ttcttcggta
     7141 ttcctaaaga ccgtttagaa aaagaagaat tagcgactga aacagccaca gtaaatgaag
     7201 tgcctgaaaa agtagtacct caaacttcag ctgaaagtgt gactatcctc tcatcagttg
     7261 ctgacggaac agttgtttct ttagataaag caagtgatcc agtttttgca caaaagatga
     7321 tgggtgaagg ctactttgta gaaccagaaa acggacatat ctattctcca gttactgggg
     7381 tagtcagctc tgtcttccca acgaaacacg ctattgggat cacaacgcca aatggtttag
     7441 aaatcttgct tcatatggga atcaacacag ttgacttagg cggtacgcca tttgatgtga
     7501 aagtaacaga agggcagcaa gtgacacctg atacgcttgt agcagaagct gacttagaag
     7561 cgatcaaagc agcaggcaaa gaaacatcca tgatcgtctt ggtaaccaat atggatcgtg
     7621 taaaaaactt tgtcttggaa aaaacaggta aagcaaaagc aaaagcacct gtgatggatg
     7681 tcaaagctta atatgataga aacaatagaa acgaaagaag aaactgaaat ctatttttca
     7741 gcttcttctt ttttattatt tattgttttc tgctaaaatc tgataaattc gtgtcacatc
     7801 agatggtgtt ccccgtaatt cataatctgc caagaaagga aagccaaatg tttgtgcata
     7861 ttgtttggct gttagacaat actgattatt gaagttttta tttccgctac cgactacacc
     7921 aagacaaagt ttatgattgt cttcatgttc cagatattca cgcatcgttt cggtcaagat
     7981 ttctgtatca ccgttatcta aaccatttcc gccgtctaaa taagtaggaa cgaaagtaaa
     8041 aaatggttct gtttctttcg taaaatcact attttcatgg atttctttta aagtaacttc
     8101 aggattgaca ggatcttctg catgcatttt ttcagcatat tctgctagat gtttggcaaa
     8161 ggcacgcgta tttcctgaaa tcgatatata taaaatgttc atggatcatc ctccaattct
     8221 ttccttttag tatagcatct tttttaagtt ttttggtggt gtaatttgga aagggactag
     8281 gcgctttgct tgtataatga gaaaaaagcc ggtagaatta tatttc
//
LOCUS       DAZKMB010000346         7829 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.guided.120, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000346 DAZKMB010000000
VERSION     DAZKMB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.guided.120"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..596
                     /locus_tag="ACG8V3_003053"
     CDS             <1..596
                     /locus_tag="ACG8V3_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0301476.1"
                     /translation="WRTILLTLISFALAIVIGVIFGLFSVAPIRALRTIASIYVDIIR
                     GIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIVRGGINAVPVGQME
                     ASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISAIGVVELLQTGKII
                     VARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            598..1335
                     /locus_tag="ACG8V3_003054"
     CDS             598..1335
                     /locus_tag="ACG8V3_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0301477.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(1506..2699)
                     /locus_tag="ACG8V3_003055"
     CDS             complement(1506..2699)
                     /locus_tag="ACG8V3_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0301478.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(2778..2984)
                     /locus_tag="ACG8V3_003056"
     CDS             complement(2778..2984)
                     /locus_tag="ACG8V3_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0301479.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(2977..4119)
                     /locus_tag="ACG8V3_003057"
     CDS             complement(2977..4119)
                     /locus_tag="ACG8V3_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0301480.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            4631..5266
                     /locus_tag="ACG8V3_003058"
     CDS             4631..5266
                     /locus_tag="ACG8V3_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0301481.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            5420..5497
                     /locus_tag="ACG8V3_003059"
                     /pseudo
     CDS             5420..5497
                     /locus_tag="ACG8V3_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            5811..5858
                     /locus_tag="ACG8V3_003060"
                     /pseudo
     CDS             5811..5858
                     /locus_tag="ACG8V3_003060"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5874..7793
                     /gene="tet(M)"
                     /locus_tag="ACG8V3_003061"
     CDS             5874..7793
                     /gene="tet(M)"
                     /locus_tag="ACG8V3_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0301482.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         2333 a         1442 c         1556 g         2498 t
ORIGIN      
        1 tttggagaac aattctccta actttgatct cctttgcttt ggctatagta atcggtgtga
       61 tttttggatt attcagtgta gcaccaatac gcgctcttcg cacaattgct agtatttatg
      121 tcgatatcat ccgaggaatt ccaatgatgg ttcttgcgtt ctttattttc tttgggttgc
      181 ctggtatcgt tggatttacg attcctgact tccttgctgg tgttatcacc ttgactctta
      241 acgcaagtgc ttatatcgca gagattgttc gaggcgggat caacgccgtt cctgtcggac
      301 aaatggaagc ttccagaagt ttaggtcttt cttataatcg gacaatgcaa aaaatcattt
      361 tgccgcaagc gatacgcatc atgatcccat cattcgtcaa tcagttcgtt atctcattaa
      421 aagacacaac gatcatttcc gcaatcggtg tagtcgaatt gctgcaaact ggtaagatca
      481 ttgttgcacg tacaacacaa agtacgtatg tttacttgat cattgcgatc atgtacttga
      541 ttttgatcac agcattgaca aaattagcaa aagttttaga aaagaaggtg aagtaagatg
      601 gcagaaaaaa tcttagttga gcatttagtc aaaaaatacg gcgataacac agtattaaac
      661 gacatcaatg tatccatcaa cgaaggagat gtcgtctgta tcattggccc ttcaggttca
      721 ggaaaaagta cattcttgcg ttgcttgaat cgtttagaag aaccttctga cggtgaaatt
      781 attattgacg gcgcccactt gatggataaa agcacagata tcaacttggt gagacaacat
      841 atcggaatgg tattccaaca ctttaaccta ttccctcatc tttctatttt ggaaaacatc
      901 attttagcac cgatggacct gaagaaagaa tctagagaag aagcagagaa aaaagcaatc
      961 aaattactgc agacagttgg tctagaagat aaaaaagata tgtatcctga aatgctttct
     1021 ggtggacaaa aacagcgtgt ggctattgct cgtgctctag cgatgaaccc tgacatcatg
     1081 ctttttgatg agcctacttc tgcactcgat cctgaaatgg tcggtgatgt cttgaacgtc
     1141 atgaaaaagt tagcagaaca aggtatgacg atggttatcg ttacgcacga aatggggttc
     1201 gctaaagaag tggccaatcg cgtcatgttc attgacgacg gaaacttttt agaagatgga
     1261 acgcctcaac aaatctttga aaatccgcaa aatgaacgga caaaagattt cttgaataaa
     1321 gtattaaata tttaaaataa attatatatg tctttttgac tatcttttta tatcttctta
     1381 aatgtccgca aagccttatt ttataggctc tacgggcatt ttgcttttgt ggtaaacctc
     1441 acatatctag gtctatcttc ttatattttc actatcaagc gtggttaaaa tcgtggtaaa
     1501 tacttctatg caattagacg ctgaacctct gattttgcgg tatctatgga cgcatgagca
     1561 taccagttca ttgtgatact gatatttgaa tgccccatga tatactgtaa atcttttggg
     1621 ttcatgttct tgcttgccag tcttgtgcag aatgtatggc gtagcgtatg cggtgtgata
     1681 tgtggcaatg gattgtcctt gtggtgcttg ttgtatttct ttaccatacg gataaataag
     1741 gcgttataat caatcgcaac tttgggcttg cccttatgat tgacaaatag gaagttgccc
     1801 cgtccgtcta tcacaaatgg ttctgccttt ggacgttttt tcataacctg ttgaaatgcc
     1861 tgtatcgttt ctttgcttaa tggtacttgc cttattccgc tttttgtttt aggcgtttca
     1921 atataatagc cctgttcctt gctctttagt aactggtggt cgataatcac aacttcattc
     1981 ttgaaatcaa tgtcggctac tgtcagtccg cacagttccg aaatacgaag tcctgtcttt
     2041 aacagtatca gcacatcatc ataatgcttg tgatacacat tgtccgtctt gatgaatgag
     2101 agtaaggctt gttcctgttc ctctgtcaat gcgactttct ttttggtatc attttctagg
     2161 acttcactca acttgaaatc aaagggattt ttcctcacat aatcatcttg aatggctatg
     2221 tagaatgacg cttttaacga gcgtttatgg ttgttaatgg tattgtagga aaagcctttg
     2281 tctttcatgc gtaacgccca ttccttagcg tcagagggtt tgattgtatc aatgctccta
     2341 gcacctaact tgtcctcttt caataaccgc atgagttgtt cccgttgttt ctgtgtgctt
     2401 ttcttcacat ttgccctctg tgcgttctgt ttggcataaa gttggcaaag cgtcattttc
     2461 ttgccagtgc tgtcgatacc gtcctcaata tcccgtctta tctgctgttc cagttctcga
     2521 agtgagggtt tttcccgttt tccctttggt gtcgggtctg tgggtgtcaa tctccaagca
     2581 tacacatatt ttgtgtttcc aaatgcgtct acatatttat ataagtattt tctgtctgtt
     2641 cgttggctct ctccagtatg caggatacga cctttgttat cccgtctttt ttctttcatg
     2701 gtgtctgctc ctttccttgt tggaaagagc cttgatatga cttgtgttca tcataacaca
     2761 tacaaggctc atttgcatta gatagcgtcc aatttatcaa tgacctgttc aaactgtcgg
     2821 cgtttaatct gtatgcggtt gccattcata atgagccagc cagcgtcctt gttttcctct
     2881 gccaatcgtc ttaacttgtt ttctccgata cggaaatact ttgacgcttc ttcaatggta
     2941 agggtgtatt tttcccatac gggaatatcc tgtgcattac tcataaaaca ccccctcact
     3001 ttcgattacg tctgctatgt ccgtacacat ctgttccaca agcatttcct gcttttcaga
     3061 gaggtgggta cattcatcaa tcattttcat gtagtttgag cctgtcagcc tgtcgatttc
     3121 ctctatcatc acgattgacg gcatgacctg tctttccagc cattgcagag tgcgttccat
     3181 attgacaggt acggggttca tttcaaaagg taaactgtca tgggaaatga atatctccca
     3241 cagcggatag tcgggaaatt gcacaaaatc agtaataagg taaaacacca gcccatgagg
     3301 gttatatgtc aataacaatt cctcaactgc ctgtactgct tttgtaccac gcagacgaat
     3361 ttcaaaacga ttgataatat ccgtatgttt ctttttggtt gcctgttcct tttgtttttc
     3421 atacaggcag aagtattttg tgcttgcctt tgaaccgata taaagggtgc ttgctaaatt
     3481 acggtttttt ccgcttagtt ttccaccctg cacattttca taatttttac agcgacaatc
     3541 cgcaccgcca tttctgtatt tttcagacaa aacgggaata tccaacagac cacacatatc
     3601 attgattgcc aagtcaaaac gtcggataac accgtcacag tcaagacagc ggtttaaaaa
     3661 tgaataccaa tctctgttct gtgcctgcaa gacatattcc atgttgcgtg agcctgcccc
     3721 ttttaattcc aagaatacgc ccatgtgttc gtcatcagaa gccatgacct tgatttctcc
     3781 gtaggcgtaa tgctctttat agccataata cccatagtca taatggataa aataatctgc
     3841 tttcatgcca agcacctttc ggataatttc caatgcgtca gtcgttggat aacggacaga
     3901 cagataatca acgagcagat aaaaaggctt atcgcagata taataattca gacaacgcag
     3961 gataatattt tgtgtttcct tgctggcgtt ggcttttccg ttctcaaagc ggttgatact
     4021 atatcttgat acatgggtaa gacttgcaag ttctccttgt gaaagaccgc ttgcaagacg
     4081 tttgttcttc atttcaagtg caaattctaa attgttcatt gtattcctcc aatcccacaa
     4141 tagccttgaa atacctgtaa acctctattc aaaaagtgtg atatgcacta tcttcttaaa
     4201 acttctcatt actactcaca actttgggat tgtatggttt ttgacttgtc ttttgtgaaa
     4261 tagtgccccc ctgttagata acgggggcac agacaaggct cgcaggctcg ccagccgaca
     4321 aagcatttct tccccttgac acaaggtgtc atggcggtaa gcctgagagg cttccgccac
     4381 cgtgtcagaa atactttgtc agcaagttct gttttgcctt ttctaacaag ttccatttca
     4441 accgatattc atatctacgg atttaatcag ataggtattt ctttttgaat gtttttatat
     4501 ttcattctat tgtgcttatt cgattacaag aacatagtaa ttcttatatg cttatttgtc
     4561 aatatctttt tttgccttta caaatcatat taagcatgat agacttataa tagcgaagat
     4621 ataacgagat atggagggtt atgcaatgaa aataagccat ttaggaaata atatacagac
     4681 cataagaaaa tttaggggaa tgaaacaaca ggaacttgcg gacaaaatcg gtatcaatat
     4741 gcagagcctt tccaagattg aaagagggtt gaactatcct gcctatgaaa cgctggaaaa
     4801 gataatggaa gtgctggacg ttacgcccaa tgaattgttg tcgggagaat ggaagtatgt
     4861 caatcaatct gaaaaggaag tctgtcagtt tttaagagcc gaagaacgtc taaatgcaga
     4921 actgaaacat ggacactatg ataacttctt tgacagcgag gaagaatggc tggaatatga
     4981 gttagaaaag ttacgggaat atattacaga ctatatcaac gggaaaagca ttgaagcgtc
     5041 cgacctttac ccaatcaaag aatttatcca gcatttgaag tttcaaaaat tattagaccg
     5101 ctatgatgat ttatacagta tggatatgtt tggggaaagc atagagggac ataagtataa
     5161 gacaccttat caagtcgtga aaatgataaa cccgaactca aaagaagata tggaactgtt
     5221 acgagaagta ttaaggaata acaattttga tgatgaggac gagtaacttt tttcctcttg
     5281 cgagaagata cagcaaacaa aaagcccgat caagagtgca aagcaccacc aatggtggca
     5341 aagctcttga cagggctttt tcttttctgt tgtgggcaat caagaggaag aaagggataa
     5401 aatctgattt gtggtaaatc aggcaaaggc aacgcaggta tctcaatttg atttggtact
     5461 tgaaaagaac gggagtaatt ggaagattat agaataacaa atattggtac attattacag
     5521 ctattttgta atcacgtact ctctttgata aaaaattgga gattccttta caaatatgct
     5581 cttacgtgct attatttaag tatctattta aaaggagtta ataaatatgc ggcaaggtat
     5641 tcttaaataa actgtcaatt tgatagtggg aacaaataat tggatgtcct tttttaggag
     5701 ggcttagttt tttgtaccca gtttaagaat acctttatca tgtgattcta aagtatccgg
     5761 agaatatctg tatgctttgt atgcctatgg ttatgcataa aaatcccagt gataaaagta
     5821 tttatcactg ggatttttat gcccttttgg gtttttgaat ggaggaaaat cacatgaaaa
     5881 ttattaatat tggagtttta gctcatgttg atgcaggaaa aactacctta acagaaagct
     5941 tattatataa cagtggagcg attacagaat taggaagcgt ggacaaaggt acaacgagga
     6001 cggataatac gcttttagaa cgtcagagag gaattacaat tcagacagga ataacctctt
     6061 ttcagtggga aaatacgaag gtgaacatca tagacacgcc aggacatatg gatttcttag
     6121 cagaagtata tcgttcatta tcagttttag atggggcaat tctactgatt tctgcaaaag
     6181 atggcgtaca agcacaaact cgtatattat ttcatgcact taggaaaatg gggattccca
     6241 caatcttttt tatcaataag attgaccaaa atggaattga tttatcaacg gtttatcagg
     6301 atattaaaga gaaactttct gccgaaattg taatcaaaca gaaggtagaa ctgtatccta
     6361 atatgtgtgt gacgaacttt accgaatctg aacaatggga tacggtaata gagggaaacg
     6421 atgacctttt agagaaatat atgtccggta aatcattaga agcattggaa ctcgaacaag
     6481 aggaaagcat aagatttcag aattgttctc tgttccctct ttatcatgga agtgcaaaaa
     6541 gtaatatagg gattgataac cttatagaag ttattactaa taaattttat tcatcaacac
     6601 atcgaggtcc gtctgaactt tgcggaaatg ttttcaaaat tgaatataca aaaaaaagac
     6661 aacgtcttgc atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag
     6721 tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat
     6781 gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt
     6841 taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc
     6901 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
     6961 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
     7021 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     7081 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     7141 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     7201 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     7261 cggtttctct tggatactta aatcaatcgt ttcaaaatgc agttatggag gggatacgct
     7321 atggctgtga acaaggattg tatggttgga atgtgacgga ctgtaaaatc tgttttaagt
     7381 atggcttata ctatagccct gttagtaccc cagcagattt tcggatgctt gctcctattg
     7441 tattggaaca agtcttaaaa aaagctggaa cagaattgtt agagccatat cttagtttta
     7501 aaatttatgc gccacaggaa tatctttcac gagcatacaa cgatgctcct aaatattgtg
     7561 cgaacatcgt agacactcaa ttgaaaaata atgaggtcat tcttagtgga gaaatccctg
     7621 ctcggtgtat tcaagaatat cgtagtgatt taactttctt tacaaatgga cgtagtgttt
     7681 gtttaacaga gttaaaaggg taccatgtta ctaccggtga acctgtttgc cagccccgtc
     7741 gtccaaatag tcggatagat aaagtacgat atatgttcaa taaaataact tagtgtattt
     7801 tatgttgtta tataaatatg gtttcttgt
//
LOCUS       DAZKMB010000347         4814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.guided.156, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000347 DAZKMB010000000
VERSION     DAZKMB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.guided.156"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8V3_003062"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8V3_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0301483.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="ACG8V3_003063"
     CDS             763..1917
                     /locus_tag="ACG8V3_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0301484.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="ACG8V3_003064"
     CDS             2132..3100
                     /locus_tag="ACG8V3_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0301485.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8V3_003065"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8V3_003065"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0301486.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="ACG8V3_003066"
     CDS             4130..4738
                     /locus_tag="ACG8V3_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0301487.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1478 a          924 c         1123 g         1289 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagcc gtattctttt agtttcttaa
     4801 aggcacttgt aata
//
LOCUS       DAZKMB010000348         4160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.guided.168, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000348 DAZKMB010000000
VERSION     DAZKMB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.guided.168"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..607
                     /gene="dfrG"
                     /locus_tag="ACG8V3_003067"
     CDS             110..607
                     /gene="dfrG"
                     /locus_tag="ACG8V3_003067"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0301488.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(679..2631)
                     /locus_tag="ACG8V3_003068"
     CDS             complement(679..2631)
                     /locus_tag="ACG8V3_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0301489.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2638..2874)
                     /locus_tag="ACG8V3_003069"
     CDS             complement(2638..2874)
                     /locus_tag="ACG8V3_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301490.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3276..3566
                     /locus_tag="ACG8V3_003070"
     CDS             3276..3566
                     /locus_tag="ACG8V3_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0301491.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(3838..4160)
                     /locus_tag="ACG8V3_003071"
                     /pseudo
     CDS             complement(3838..4160)
                     /locus_tag="ACG8V3_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1294 a          714 c          730 g         1422 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcat aatgagaaaa ggttttttga
       61 tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa tgaaagtttc
      121 tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca ttccttggag
      181 gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga taatattagg
      241 taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata ttattctgac
      301 gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag aagatgtttt
      361 tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga tttataattt
      421 gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat tcgaaggaga
      481 tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc aaaaagggat
      541 aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa aaaacttatt
      601 gagttaacgg gtgcgttaat tgaagaagaa cagtaaagat aaagcacaag agcttgggat
      661 tactccaagc tctttttgtc atttgaaata aattatatct ttccaatcaa attgtttatc
      721 tgtaatccct aatctttgag caggtgtcaa aaccttttta tccgctgatt taaaagggcg
      781 acaaaagttg tagtaagttc ttagaattgt aatcgcaaat tgagcatact tcggattgaa
      841 gttagcgtaa atatagctct tcttatcacc acgagctgtt gttaatggtc tttcaagaga
      901 tgatatataa cgtcttatct gttgaataaa gctattggtc gaatggtcat taacatttaa
      961 tagcatcttt gcgatttcgt ttggttccag agcagataaa tccgttctac aatcaactga
     1021 ataaaatcct ttatccttag tggctaatgg atgtttgata gggttatcgg catatttgcg
     1081 atatctttct ccatctttac taatagcctc ttcgtggaat tgatgacttg ttagtaattc
     1141 agttagataa tgagatgcca aagtccgtag aaactttgtt ttgaatcctc tgatatctcc
     1201 ccaatcgagc agttctgctt ttgcttgtcc gaattctttt aagcattgtt ttctgctttt
     1261 cgtcttgtta acttggcaaa taaaatgatg tgcatcgctc aaccttaatt ctttcgtgaa
     1321 tactctataa aaggcgtttc taatagagaa gtcatcatca ctaatcattc gccattcgtc
     1381 ggcattaacc atttgtttaa ttaaccaata gtgagcagtt gtagtataag gagcatcaac
     1441 atttaaacca tcgacaaatc tactcctatt agtaattttc cctaattcgt gaagataagc
     1501 attcctattt tcgctatcgt tagcggatgg ttcttgtgga taataagacc aatctaacct
     1561 atcattttta cgactaaaag tattgagatg gtcttctttg aattttcttg tatcttcatt
     1621 taattcatcc attgaaatat tccaatcata ggcaacatca gaacggaaca catatcttga
     1681 taagacctca gcagatacaa ctatatacgt ttgcatatta aggtcttcaa accccgtata
     1741 tttctttgaa ccttgtcctt tcttacggac gttatttagg tagtagtgca tcttgtccgt
     1801 attaagccac atctcgttaa atttctttgt ttgaaggggt tgtgtctcat atcgttctaa
     1861 aaactccaaa caacgcctat acagccactc cagcttatga tagtaggttc caactccaat
     1921 tcctaaaata tcacaagtac gactgactga aacccttcca acaaccatct tagcgaacat
     1981 tggaagaatg gtgttctttt gttggtgata agtagtggtt tcttctcttt ttggtagaac
     2041 attagtgaac ttcttacacg ccttacattg atagcgttga gatttgcccc tacttttccc
     2101 ccttttgtaa aattgcttcg gctcattgaa tggggttgat tcttcttccg agcagccttc
     2161 tttatgaaat tgataatctg gctcaacatc ttggatgcta ttgatttcta taaggcgttt
     2221 aatttcttca acaacagacc aattagataa aggggttact gtacagtttt gcgatacacc
     2281 tctgccaata ggattgtcat tgcagtacat acctttatga cctttatctt tggaactacc
     2341 agtcatttta tatcgacttg gtttcccttt tacatctttg aatttgtgct gttctttccc
     2401 aaaattacaa caaaacggat tgatgcagta attatattga atgggatatt cagaaccgtt
     2461 ccatttaaaa gacgttggca agaacaacat tttgttatac cttgttgcaa atcgttttgg
     2521 agttaataaa tcatattgtt tagccctatc ttttaattct tcgtttgaaa tcggaatttt
     2581 aacatctaca atttcatctt ccttcgtggc taatctttta agcttggtca ttgtctatca
     2641 acccactcta tctttgatac ccattttttc aacaatccgc ttattacttc gcttgttggc
     2701 aatccattca ataaacttct tatcgtctaa aatgttcaat aagaattcat ccactgcttc
     2761 gctttcagta cgctctgcat attcagcgta ttgcttaact atcaccctta cttgctctga
     2821 tatttcccaa tccacagact tagcgttctt attttttggc tcaagaaatt gcataggaaa
     2881 atgcacccct ttttgcttag taaattgtaa ccattttaac acaccaattt gcattcctac
     2941 aataattatt tcacgttcgc atttttatat acattttaac ttacattttg tggtgtaaat
     3001 taacaacaaa cgttgattta acaataaaaa acgagtatca aattcagttg caatttgata
     3061 ctcatttagg tttacatttt cactttttgt actccaaaac tgaactactt aaatctatac
     3121 tgctgcctct tttttttagt acatgtcctt catcttcgca ggaagtgcca atatgatttt
     3181 aagaaaggta atagacaaaa agtcaaaaag ataaaacaga gtttcttctt tacaaaggga
     3241 atgtatgttc gtataatggt attgaggtga aggttatgga gaaaaaatat ggaacagtag
     3301 ataagatccg tactttgcaa gttagtgcca gaccgttgat ccgtttcagt ttaaatgggg
     3361 tcaattgttt gattgcttcc catagtttga acttcctagc tgaagtagac gaagggatga
     3421 agttagtcgt tacaggttat tataataata gaaagcaatt tgttgtgaga tcctatcatg
     3481 tgttagggaa acccaaaatt gtggtagaat atgagaaaag tctataccca agaaaaaagg
     3541 tccgacaaga tgaaagaata aactagaaaa caagcggctg agacaaaaat gtcctagccg
     3601 cttgttttgg aaagatggat gcagacgatg ggatttgaac ccacacgagc atacgctcac
     3661 aagaacctca atctatatat aactacatgt atttaagtat ttgtgatgaa tcactaaact
     3721 ttccaaactc atttgaggga tattgcttaa agattgaaca gatcaattcg aagccctatt
     3781 agtattcgct caatgggggg atcagaggcc ataaaaagag actagtcatt actagtctca
     3841 ggtagtggaa ttgtaggatt ttaaacgttc ctggagagta ccggtatgta actcgaagag
     3901 gtgattatca tagtcgtaaa agtaaattga agaagcctcg cctttcacac gagaacgtcc
     3961 tggttcgaca gttaaaccta acatttggat ttttgagaca aacgaatcta tatcttgttc
     4021 gtcaatttta aaagcaatat gattgtatgt ctttggcagt ttattcgacg aattctgcat
     4081 tactgcaatc cataggtcct taactaagaa aaatttttct ggatataatg aaaatttttt
     4141 tgttgactat cgtagatttc
//
LOCUS       DAZKMB010000349         3278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.guided.182, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000349 DAZKMB010000000
VERSION     DAZKMB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.guided.182"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..188
                     /locus_tag="ACG8V3_003072"
     CDS             3..188
                     /locus_tag="ACG8V3_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0301492.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="ACG8V3_003073"
     CDS             203..1072
                     /locus_tag="ACG8V3_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0301493.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="ACG8V3_003074"
     CDS             1053..1787
                     /locus_tag="ACG8V3_003074"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0301494.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="ACG8V3_003075"
     CDS             1820..2728
                     /locus_tag="ACG8V3_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0301495.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="ACG8V3_003076"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="ACG8V3_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAZKMB010000350         2275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.guided.203, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000350 DAZKMB010000000
VERSION     DAZKMB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.guided.203"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..1170
                     /locus_tag="ACG8V3_003077"
     CDS             259..1170
                     /locus_tag="ACG8V3_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0301496.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1323..1808
                     /gene="vanZ-A"
                     /locus_tag="ACG8V3_003078"
     CDS             1323..1808
                     /gene="vanZ-A"
                     /locus_tag="ACG8V3_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0301497.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          800 a          331 c          428 g          716 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattc aaggaggtaa ggatggcgga atgaaaccaa cgaaattaat
     1201 gaacagcatt attgtactag cacttttggg gtaacgttag ctttttaatt taaaacccac
     1261 gttaactagg acattgctat actaatgata caacttaaac aaaagaatta gaggaaatta
     1321 tattgggaaa aatattatct agaggattgc tagctttata tttagtgaca ctaatctggt
     1381 tagtgttatt caaattacaa tacaatattt tatcagtatt taattatcat caaagaagtc
     1441 ttaacttgac tccatttact gctactggga atttcagaga gatgatagat aatgttataa
     1501 tctttattcc atttggcttg cttttgaatg tcaattttaa agaaatcgga tttttaccta
     1561 agtttgcttt tgtactggtt ttaagtctta cttttgaaat aattcaattt atcttcgcta
     1621 ttggagcgac agacataaca gatgtaatta caaatactgt tggaggcttt cttggactga
     1681 aattatatgg tttaagcaat aagcatatga atcaaaaaaa attagacaga gttattattt
     1741 ttgtaggtat acttttgctc gtattattgc tcgtttaccg tacccattta agaataaatt
     1801 acgtgtaaga tgtctaaatc aagcaatctg atctttcata cacataaaga tattgaatga
     1861 attggattag atggaaaacg ggatgtgggg aaactcgccc gtaggtgtga agtgagggga
     1921 aaaccggtga taaagtaaaa agcttaccta acactatagt aacaaagaaa gcccaattat
     1981 caattttagt gctgaggaat tggtctcttt aataaatttc cttaacgttg taaatccgca
     2041 ttttcctgac ggtaccccca ctagcagcca tgcttttttt gaatctttgt aggacgtttt
     2101 aaagagaatt acccacttta aaattaaaca tgcgcttaga atcgctttag gaaacacgat
     2161 ccagtccaat aatcgtcgat aaaaactttt gaaaaaggtt ggtgaaatta cctacttttg
     2221 gaataatcac aaatcacaag tgattaatca cttgtttatt aagatattaa aagct
//
LOCUS       DAZKMB010000351         1148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.guided.259, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000351 DAZKMB010000000
VERSION     DAZKMB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.guided.259"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..76
                     /locus_tag="ACG8V3_003079"
     CDS             26..76
                     /locus_tag="ACG8V3_003079"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0301498.1"
                     /translation="MWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8V3_003080"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8V3_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0301499.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          199 c          185 g          343 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
     1081 caaattccca ctaagcgctc gggacccctt gtaggaaaat gtcctaagtg tggcaacaat
     1141 attgtatt
//
LOCUS       DAZKMB010000352          944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.guided.272, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000352 DAZKMB010000000
VERSION     DAZKMB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.guided.272"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            228..776
                     /gene="aac(6')"
                     /locus_tag="ACG8V3_003081"
     CDS             228..776
                     /gene="aac(6')"
                     /locus_tag="ACG8V3_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0301500.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          333 a          141 c          201 g          269 t
ORIGIN      
        1 aagataatct atatcatgat gaacaaacag agttttttcc tccaattgat ggctttcctg
       61 atctttccta ttttgaataa agtgtttctg tataattttt ttattttata agtaagataa
      121 ataatgaaag aatagagata ctgattggta gctaaagatt tttgagcgag aacatttcgt
      181 tttttcaaaa ggactgttag aataagcaag aacaaggggg aagacgtatg ataatcagtg
      241 aatttgaccg taataatcca gtattgaaag atcagctttc tgatttactg agactgactt
      301 ggccggaaga atatggagac agctcggcag aagaagtaga agaaatgatg aatccagaac
      361 gaatcgcggt agcagcggta gaccaagatg agttagtagg atttattggt gcaatccctc
      421 aatacggtat cacaggttgg gaattgcatc cattagttgt agaaagctcc cgacgaaaga
      481 accaaatagg tactcgatta gtcaattact tagaaaaaga agtagcttcc agaggaggaa
      541 tcacgattta tttaggtacg gatgatttag accatggaac aacgttaagt caaacggacc
      601 tgtatgagca tacatttgat aaagtggctt ccatccagaa ccttcgtgaa catccgtatg
      661 aattctatga aaaattaggt tataaaatcg taggtgtctt accaaatgca aatggctggg
      721 acaaaccgga tatttggatg gcaaaaacga ttattcctcg accaaattct caataataaa
      781 aagaggttgt gacagaagta atcagcttca agaaataaga aggaatttac gaaaattact
      841 tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag ctacttttgg aacaccgtgt
      901 accagaggtt gtgaaaaagt cgcttagttt cgagtagtaa gaaa
//
LOCUS       DAZKMB010000353          890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3700
            SAMEA115706879-rid22579913.guided.277, whole genome shotgun
            sequence.
ACCESSION   DAZKMB010000353 DAZKMB010000000
VERSION     DAZKMB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706879
            Sequence Read Archive: ERR13196613
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287808.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287808.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:35:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,082
            CDSs (total)                      :: 3,020
            Genes (coding)                    :: 2,923
            CDSs (with protein)               :: 2,923
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706879-rid22579913.guided.277"
                     /isolate="VRE3700"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..861
                     /locus_tag="ACG8V3_003082"
     CDS             214..861
                     /locus_tag="ACG8V3_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0301501.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
BASE COUNT          351 a           86 c          133 g          320 t
ORIGIN      
        1 atgaaatcga ctttaaaagc gaacgaaaaa caattgcaaa agcagattga tttttggcaa
       61 cgtgaattta ggttttggaa gtaaaataag ttttatttga taaaaattgc taattcagta
      121 taattaatat ttacgaggtg acataacgta tgaaaaaatc agaggattat tcctcctaaa
      181 tataaaaatt taaaatttag gaggaagtta tatatgactt ttaatattat tgaattagaa
      241 aattgggata gaaaagaata ttttgaacac tattttaatc agcaaactac ttatagcatt
      301 actaaagaaa ttgatattac tttgtttaaa gatatgataa aaaagaaagg atatgaaatt
      361 tatccctctt taatttatgc aattatggaa gttgtaaata aaaataaagt gtttagaaca
      421 ggaattaata gtgagaataa attaggttat tgggataagt taaatccttt gtatacagtt
      481 tttaataagc aaactgaaaa atttactaac atttggactg aatctgataa aaacttcatt
      541 tctttttata ataattataa aaatgacttg cttgaatata aagataaaga agaaatgttt
      601 cctaaaaaac cgatacctga aaacaccata ccgatttcaa tgattccttg gattgatttt
      661 agttcattta atttaaatat tggtaacaat agcagctttt tattgcctat tattacgata
      721 ggtaaatttt atagtgagaa taataaaatt tatataccag ttgctctgca acttcatcat
      781 tctgtatgtg atggttacca tgcttcacta tttatgaatg aatttcaaga tataattcat
      841 agggtagatg attggattta gtttttagat tttgaaagtg aatttaattt
//
